LOCUS       DAYYME010000001        71211 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000001 DAYYME010000000
VERSION     DAYYME010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 71211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.001"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            189..419
                     /locus_tag="ACHBFW_000001"
     CDS             189..419
                     /locus_tag="ACHBFW_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFM9316353.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            516..1817
                     /gene="murA"
                     /locus_tag="ACHBFW_000002"
     CDS             516..1817
                     /gene="murA"
                     /locus_tag="ACHBFW_000002"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM9316354.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1830..2006
                     /locus_tag="ACHBFW_000003"
     CDS             1830..2006
                     /locus_tag="ACHBFW_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM9316355.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2141..2437
                     /gene="yidD"
                     /locus_tag="ACHBFW_000004"
     CDS             2141..2437
                     /gene="yidD"
                     /locus_tag="ACHBFW_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFM9316356.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2622..3089
                     /locus_tag="ACHBFW_000005"
     CDS             2622..3089
                     /locus_tag="ACHBFW_000005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9316357.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3174..4058
                     /locus_tag="ACHBFW_000006"
     CDS             3174..4058
                     /locus_tag="ACHBFW_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9316358.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4172..4408
                     /locus_tag="ACHBFW_000007"
     CDS             4172..4408
                     /locus_tag="ACHBFW_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFM9316359.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4496..4576
                     /locus_tag="ACHBFW_000008"
     tRNA            4496..4576
                     /locus_tag="ACHBFW_000008"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4530..4532,aa:Tyr,seq:gta)
     gene            4583..4654
                     /locus_tag="ACHBFW_000009"
     tRNA            4583..4654
                     /locus_tag="ACHBFW_000009"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4615..4617,aa:Gln,seq:ttg)
     gene            4779..4949
                     /locus_tag="ACHBFW_000010"
     CDS             4779..4949
                     /locus_tag="ACHBFW_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316360.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5169..5876
                     /locus_tag="ACHBFW_000011"
     CDS             5169..5876
                     /locus_tag="ACHBFW_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9316361.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5935..6579)
                     /locus_tag="ACHBFW_000012"
     CDS             complement(5935..6579)
                     /locus_tag="ACHBFW_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFM9316362.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6705..6992
                     /locus_tag="ACHBFW_000013"
     CDS             6705..6992
                     /locus_tag="ACHBFW_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316363.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7100..7420
                     /locus_tag="ACHBFW_000014"
     CDS             7100..7420
                     /locus_tag="ACHBFW_000014"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9316364.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7501..8814
                     /locus_tag="ACHBFW_000015"
     CDS             7501..8814
                     /locus_tag="ACHBFW_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM9316365.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8811..9530
                     /locus_tag="ACHBFW_000016"
     CDS             8811..9530
                     /locus_tag="ACHBFW_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFM9316366.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9658..10209
                     /gene="raiA"
                     /locus_tag="ACHBFW_000017"
     CDS             9658..10209
                     /gene="raiA"
                     /locus_tag="ACHBFW_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFM9316367.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10431..12965
                     /gene="secA"
                     /locus_tag="ACHBFW_000018"
     CDS             10431..12965
                     /gene="secA"
                     /locus_tag="ACHBFW_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFM9316368.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13091..14192
                     /gene="prfB"
                     /locus_tag="ACHBFW_000019"
     CDS             join(13091..13162,13164..14192)
                     /gene="prfB"
                     /locus_tag="ACHBFW_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFM9316369.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14297..14983
                     /gene="ftsE"
                     /locus_tag="ACHBFW_000020"
     CDS             14297..14983
                     /gene="ftsE"
                     /locus_tag="ACHBFW_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFM9316370.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14976..15860
                     /gene="ftsX"
                     /locus_tag="ACHBFW_000021"
     CDS             14976..15860
                     /gene="ftsX"
                     /locus_tag="ACHBFW_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFM9316371.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            16012..17460
                     /gene="cls"
                     /locus_tag="ACHBFW_000022"
     CDS             16012..17460
                     /gene="cls"
                     /locus_tag="ACHBFW_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM9316372.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17797..18654
                     /locus_tag="ACHBFW_000023"
     CDS             17797..18654
                     /locus_tag="ACHBFW_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFM9316373.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18769..19689
                     /gene="pstC"
                     /locus_tag="ACHBFW_000024"
     CDS             18769..19689
                     /gene="pstC"
                     /locus_tag="ACHBFW_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFM9316374.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTITTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19689..20573
                     /gene="pstA"
                     /locus_tag="ACHBFW_000025"
     CDS             19689..20573
                     /gene="pstA"
                     /locus_tag="ACHBFW_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFM9316375.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20588..21394
                     /gene="pstB"
                     /locus_tag="ACHBFW_000026"
     CDS             20588..21394
                     /gene="pstB"
                     /locus_tag="ACHBFW_000026"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM9316376.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21410..22168
                     /gene="pstB"
                     /locus_tag="ACHBFW_000027"
     CDS             21410..22168
                     /gene="pstB"
                     /locus_tag="ACHBFW_000027"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM9316377.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22181..22858
                     /gene="phoU"
                     /locus_tag="ACHBFW_000028"
     CDS             22181..22858
                     /gene="phoU"
                     /locus_tag="ACHBFW_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFM9316378.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23435..24127
                     /locus_tag="ACHBFW_000029"
     CDS             23435..24127
                     /locus_tag="ACHBFW_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM9316379.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            24124..24891
                     /locus_tag="ACHBFW_000030"
     CDS             24124..24891
                     /locus_tag="ACHBFW_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM9316380.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24991..26559
                     /gene="liaX"
                     /locus_tag="ACHBFW_000031"
     CDS             24991..26559
                     /gene="liaX"
                     /locus_tag="ACHBFW_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFM9316381.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26856..27176
                     /locus_tag="ACHBFW_000032"
     CDS             26856..27176
                     /locus_tag="ACHBFW_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFM9316382.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPFARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27176..27526
                     /locus_tag="ACHBFW_000033"
     CDS             27176..27526
                     /locus_tag="ACHBFW_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFM9316383.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27693..28631
                     /gene="hprK"
                     /locus_tag="ACHBFW_000034"
     CDS             27693..28631
                     /gene="hprK"
                     /locus_tag="ACHBFW_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFM9316384.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANKNGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28646..29476
                     /gene="lgt"
                     /locus_tag="ACHBFW_000035"
     CDS             28646..29476
                     /gene="lgt"
                     /locus_tag="ACHBFW_000035"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFM9316385.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29520..30545
                     /locus_tag="ACHBFW_000036"
     CDS             29520..30545
                     /locus_tag="ACHBFW_000036"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM9316386.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30560..31498
                     /gene="galU"
                     /locus_tag="ACHBFW_000037"
     CDS             30560..31498
                     /gene="galU"
                     /locus_tag="ACHBFW_000037"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM9316387.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31623..32063
                     /locus_tag="ACHBFW_000038"
     CDS             31623..32063
                     /locus_tag="ACHBFW_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFM9316388.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32066..32668
                     /locus_tag="ACHBFW_000039"
     CDS             32066..32668
                     /locus_tag="ACHBFW_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM9316389.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32727..33833)
                     /locus_tag="ACHBFW_000040"
     CDS             complement(32727..33833)
                     /locus_tag="ACHBFW_000040"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFM9316390.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34043..35044
                     /gene="ccpA"
                     /locus_tag="ACHBFW_000041"
     CDS             34043..35044
                     /gene="ccpA"
                     /locus_tag="ACHBFW_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFM9316391.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35381..37726)
                     /locus_tag="ACHBFW_000042"
     CDS             complement(35381..37726)
                     /locus_tag="ACHBFW_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFM9316392.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            38005..38907
                     /locus_tag="ACHBFW_000043"
     CDS             38005..38907
                     /locus_tag="ACHBFW_000043"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFM9316393.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            39120..40895
                     /locus_tag="ACHBFW_000044"
     CDS             39120..40895
                     /locus_tag="ACHBFW_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFM9316394.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40961..42547)
                     /locus_tag="ACHBFW_000045"
     CDS             complement(40961..42547)
                     /locus_tag="ACHBFW_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFM9316395.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            42798..43022
                     /locus_tag="ACHBFW_000046"
     CDS             42798..43022
                     /locus_tag="ACHBFW_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFM9316396.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43163..44914
                     /locus_tag="ACHBFW_000047"
     CDS             43163..44914
                     /locus_tag="ACHBFW_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM9316397.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTK
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIVMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPVKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44914..46698
                     /locus_tag="ACHBFW_000048"
     CDS             44914..46698
                     /locus_tag="ACHBFW_000048"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9316398.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRTTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVLVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            47024..47875
                     /gene="licT"
                     /locus_tag="ACHBFW_000049"
     CDS             47024..47875
                     /gene="licT"
                     /locus_tag="ACHBFW_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM9316399.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47971..49851
                     /locus_tag="ACHBFW_000050"
     CDS             47971..49851
                     /locus_tag="ACHBFW_000050"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM9316400.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVSFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYIMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVRQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(49891..50793)
                     /locus_tag="ACHBFW_000051"
     CDS             complement(49891..50793)
                     /locus_tag="ACHBFW_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9316401.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            51024..52427
                     /locus_tag="ACHBFW_000052"
     CDS             51024..52427
                     /locus_tag="ACHBFW_000052"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM9316402.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52427..53860
                     /locus_tag="ACHBFW_000053"
     CDS             52427..53860
                     /locus_tag="ACHBFW_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM9316403.1"
                     /translation="MGFRKDFLWGGATAANQCEGAYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSNN"
     gene            54005..54931
                     /gene="trxB"
                     /locus_tag="ACHBFW_000054"
     CDS             54005..54931
                     /gene="trxB"
                     /locus_tag="ACHBFW_000054"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFM9316404.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55145..56872
                     /locus_tag="ACHBFW_000055"
     CDS             55145..56872
                     /locus_tag="ACHBFW_000055"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFM9316405.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56887..57237
                     /locus_tag="ACHBFW_000056"
     CDS             56887..57237
                     /locus_tag="ACHBFW_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFM9316406.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(57329..58189)
                     /locus_tag="ACHBFW_000057"
     CDS             complement(57329..58189)
                     /locus_tag="ACHBFW_000057"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFM9316407.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            58281..58709
                     /locus_tag="ACHBFW_000058"
     CDS             58281..58709
                     /locus_tag="ACHBFW_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM9316408.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58871..59047
                     /gene="rpsU"
                     /locus_tag="ACHBFW_000059"
     CDS             58871..59047
                     /gene="rpsU"
                     /locus_tag="ACHBFW_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFM9316409.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            59074..59520
                     /locus_tag="ACHBFW_000060"
     CDS             59074..59520
                     /locus_tag="ACHBFW_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFM9316410.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(59674..61053)
                     /gene="brnQ"
                     /locus_tag="ACHBFW_000061"
     CDS             complement(59674..61053)
                     /gene="brnQ"
                     /locus_tag="ACHBFW_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM9316411.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            61759..62730
                     /locus_tag="ACHBFW_000062"
     CDS             61759..62730
                     /locus_tag="ACHBFW_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFM9316412.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            62754..64946
                     /locus_tag="ACHBFW_000063"
     CDS             62754..64946
                     /locus_tag="ACHBFW_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFM9316413.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            64963..65439
                     /gene="ybeY"
                     /locus_tag="ACHBFW_000064"
     CDS             64963..65439
                     /gene="ybeY"
                     /locus_tag="ACHBFW_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFM9316414.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            65423..65818
                     /locus_tag="ACHBFW_000065"
     CDS             65423..65818
                     /locus_tag="ACHBFW_000065"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM9316415.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            65833..66732
                     /gene="era"
                     /locus_tag="ACHBFW_000066"
     CDS             65833..66732
                     /gene="era"
                     /locus_tag="ACHBFW_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFM9316416.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            66875..67687
                     /gene="recO"
                     /locus_tag="ACHBFW_000067"
     CDS             66875..67687
                     /gene="recO"
                     /locus_tag="ACHBFW_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFM9316417.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            68071..68988
                     /gene="glyQ"
                     /locus_tag="ACHBFW_000068"
     CDS             68071..68988
                     /gene="glyQ"
                     /locus_tag="ACHBFW_000068"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFM9316418.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            68990..71065
                     /gene="glyS"
                     /locus_tag="ACHBFW_000069"
     CDS             68990..71065
                     /gene="glyS"
                     /locus_tag="ACHBFW_000069"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFM9316419.1"
                     /translation="MAKDLLLEIGLEEIPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKQYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        23285 a        12469 c        15245 g        20212 t
ORIGIN      
        1 atttggaata agggactgcg acaagacttt tgtctcagtc cctttttttg catattttta
       61 gcatttattt cctaaaaaat gtaaggggat tcctatattt ttaagtatac ttgtttttcc
      121 ttttatacat tttatggtat tatcatttta ttagttatta taaaacaatc gttagaaagg
      181 acgtcattat gcaagtatac ggaattgatg cggtgcttcg aatcgtgagt catttggcgt
      241 ttatctactt agccttctgg tctttacgtt cgttacgcat tgaaactttt ttcagaccaa
      301 tgcataatac gcaaattaga atggcaatcg tacttttttc aattttttta gggtacactg
      361 caagtaattt tttcttagaa gttatcgctt tatgccgaaa tcttttcgtt agcctgtaaa
      421 ttttaaaaaa aggtactatt ttaaaagcaa ggatgttata atagaaaaga ttttgagtaa
      481 gtgtatgctc attggaattt tttggaggga acaacatgga agagatcatc gtaagaggtg
      541 gcaatcaatt aaatggaaca gtaagaattg aaggagctaa aaatgctgtt ttgcctattt
      601 tagctgctag tttgttagca gaagaaggta ttacgacatt agacaacgtt ccgattcttt
      661 ctgatgtctt cactatgaat caagtgattc gtcacttaaa cgtagatgta gattttgatg
      721 aacaaaagaa tcaagtaaca atcgacgctt caagacaatt agaaatcgaa gcgccttatg
      781 agtatgttag tcaaatgcgt gcttcaatcg ttgtgatggg tccgctactt gccagaaatg
      841 gacatgcaaa agtagctatg cctggtggat gtgcgattgg taaacgacca atcgatttgc
      901 atttaaaagg atttcaagct ttaggagcaa aaattattca aaaaaacggc tatatcgaag
      961 caattgcgga tgaattgatc ggtaacacga tttatttaga tttccctagt gtaggtgcta
     1021 ctcaaaatat catgatggct gccgtaaaag caaaaggaac aaccatcatt gaaaacgtag
     1081 ctcgcgaacc agaaatcgtt gatttagcaa acattttgaa taaaatgggc gctcaagtct
     1141 atggagctgg tactgaaaca atgcgtattg aaggcgtgga tcatttacat gcagtcaacc
     1201 actcaatcgt acaagatcgt atcgaagcag gtacctttat ggttgctgct gcaatgacac
     1261 aaggcaatgt gctgattgca gatgctatct ctgaacataa ccgcccattg atttctaaat
     1321 tgatcgaaat gggtgcagaa atcattgaag aagaaggcgg agtccgtgtc atcggaccaa
     1381 aacatatctt acctacagat gtaaaaacga tgcctcatcc tggtttccct acagatatgc
     1441 aagctcaaat gacagctatc caattagttg cagaaggtac aagtgtagtg actgaaacag
     1501 tgtttgaaaa tcgtttccaa catttagaag agatgcgccg tatgaacgct catgtcaaaa
     1561 tcgacggaaa tgtagcaatc atggatggaa accatgaatt gcaaggtgca gaagtatatg
     1621 caacagactt gcgggcagca gcagcattag ttttagctgg cttgaaagca aatggaatca
     1681 cacgtgtgcg taacttgaat tatctagacc gtggatatta taatttccat atcaaactac
     1741 agcaattagg tgcagacgtt gaacgtgtag acatggatca aacttctgct gaaaaaacag
     1801 cacaaacgat tgcttaagga agtggctgaa tgagtaacca acgatatatc ttattaactt
     1861 tgataaagat tttaatggtg atcttattgc ttatccttct ttttgtggcc ggaacgatga
     1921 tcggatatgg tgtcatcggt ggcggcaatc catttaaagt atttcagcca agtttgtgga
     1981 ttcatattcg agactttttt cattgataaa taagaatcac agtggttcta gtcgatatga
     2041 agtgagccat gaagcagaac atgataaaag aaaacagaag ggaacgttga cgttagtcgc
     2101 gatccctttt tttagtaaaa gaaaagaagg aatctaattg atgaagaaga tattgattta
     2161 tttagttaga ggatatcagc gctttatttc gcctgcattt ccacctagct gccgttatta
     2221 cccaacctgt tccaattata tgataaaagc aattcaaacc catggcgcgc taaaagggac
     2281 ggtaatgggg actgcacgga tcttacgctg tcatcctttt gtcaaaggcg gtatcgatta
     2341 tgtaccattg acattccgat taacgcgaaa taagaaagat aaaaaagaac cagattataa
     2401 aaaggttgtg aaaaatacgg gtagcactga gcgataaaga aacaatttgg aaaatagctt
     2461 cttatatttt gcaaaattgt gagttattgc cgaagtgttg gattttgaac accgtcaaaa
     2521 aaaaggttgt gaaaaatacg gctagcactg agcgataaag aaaaagagga aatttattgt
     2581 caaagtaaca actccgcaac tacttatatg ggggacaaaa aatgatcgaa gaactaaaca
     2641 agaaagattt accatggtcc ttgttattag atgcagatcc tgataaagaa aaagttcaag
     2701 tatatgtttc cagagggagt ggctggatat ggaaagaaaa agaaaaaaca ataggtgtac
     2761 tgatctatgt ggcaagggaa aaagaatacg aaatcgtcaa tgtagcagta accccaacgt
     2821 atcaaggaaa ggggattggc ggaaaattac ttgagacagc ttttcagaag ctttcacaac
     2881 tcgcttccag tcaaactcag atcatcatac gaaccggcag tactagcagt gcggcgttac
     2941 atctctatca aaaaatgggc tatgtggaga ttggtcgagt caaagactat tttatccatc
     3001 attactctga gcccattttt gaaaatggag atcaattaag ggatcaagtg attttagcaa
     3061 ttcacttacc taaaaatagc tttaactaat aaaatttcaa ttcaagaaaa tgctttcaaa
     3121 tgctaaatag atacattaca atagctttag caaacaaaaa ggagaatatt tttatgggaa
     3181 tattagtttt tgattttggt ggatcggctg tcaaatatgg ttgttgggat ggcaaagaga
     3241 ttaaaggtaa gggacaattt gcaacgcctg aatcttggga agaaatgaaa gcgcaattat
     3301 ttcaagttta taaaaaaata ctcgtttctt ttgaagtcga aggaattgga atcagctctc
     3361 ctggagtagt gaataatgag aaacaggtga ttgagggaat cagtgcgatt ccttatattc
     3421 atggatttaa tattttccga gatttggaaa ctctgtttca acttccagta acgattgaaa
     3481 atgacgccaa ctgtgccgga atggctgaat tttatgaagg tgctgcccgc gattatcaag
     3541 atgttgcttt tgttgtggca ggaactggga ttggcggggc gttgttcacg aaaggacaaa
     3601 tcaataaagg ggcacatctt tacggtggag aatttgggtt gatgttctta aatggcgata
     3661 agacattcag taaacttggt acagcggtac agatggcttg gcgttattgt gaacgcaaag
     3721 gtctcgataa aaatgcttat accggaaaag atgtctttga attagcagaa actggagatg
     3781 caatagcaaa agaagaagtt gagagttttt atacgtatct tacaaaggga ttattcagta
     3841 ttcaattttc gtttgatcca gaagtgatcg tattaggcgg cggggtttct gcaaaagaag
     3901 ggctcattga tgagatcaaa agccgaatga agaagctgac agaacaattc gatctgcatg
     3961 atttcgaacc gcagatttta ttatgtgaat acagaaacga tgctaatctt gtaggtgcag
     4021 cagcaaacta tctcgctgta cgtcaagggg agaattaaga gctttagaga aggtgcagct
     4081 tgcataaaag aagtgaatga aatgaatagg agatttttac ctaaggaatg ttttatttgc
     4141 tgcagtatga taaaatatct gagaggtgaa aatgatgtca aaaaaaaata aagatattga
     4201 agtgcgtata gaagaagtga aaaaagatat caaaggtaag aattatgaag tcactcagtt
     4261 atttataggg aaaaaaatga ttggtgagat cttagcctat ggaccaaaag aatatgaaat
     4321 tttctttggt gaagaagatt ttggaaaaga aaaaagtctt gaaaatgcga tagaaacagt
     4381 gattcgacac tggaatctgc acgaataaaa aaatatcaaa aaaactttca ataaagggtt
     4441 gcaaaaatct ggaaaattag gtattataac taagtcaggt tgatacaaac ctttcggagg
     4501 agtagcgaag tggctaaacg cgacggactg taaatccgtt ccttagggtt cagtggttcg
     4561 aatccactct cctccatttc attggggtat agccaagcgg taaggcaagg gactttgact
     4621 ccctcatgcg ttggttcgaa tccagctacc ccagtatacc acaatcgtaa gctctcgtta
     4681 gttcgagagc tctttttttt gctttgctac cttgttcctt gaagattctg ctgatttaag
     4741 atagagttaa gttaactaca agaattggag ggatccacat gaaatggaaa gaaaaagtag
     4801 acgaagcagc tgaaaagtta tatgatttgg tcaaatcaga aaaatacaat attgaagtga
     4861 atattccgaa aaaaggcgga aaagcggtcc gggtaaaatc aaaacgccca acgaatcata
     4921 caaaaaaatg gatggctaaa aaccgctaat aataacaaaa gatccataag tcggcatttt
     4981 catcgatttt agggtctttt gttattttct aggttggaca tatctaaaaa attgtttctg
     5041 acaataggag taggaagaac gatgtgtgtc taaatcctct gttgatggat agaaaccttt
     5101 atctttttca gttgtgaatc aggtatatac cagttataat agaagaaacg aaaggacggg
     5161 tgtacgttat gtcgaatcaa ttacctgtat atatacagat tcatgaccaa ttaaaaaatg
     5221 aaatcgaaca aggtatatgg aaagtcggag accgtcttcc ttcagaaaga gaattagcag
     5281 tgaaattcaa tgtcagccgt atgacgttga gacaagcaat tcaaaacttg gcagatgaag
     5341 gaatcttaga aagaaagatc ggttcgggta catatgttgc acgagaaaaa gtacaagaaa
     5401 aaatgtctgg tgcaacaagt tttacagaaa ttatggaatc acaaaatcga gtaccgtcta
     5461 gtcggacaat ctcttatttc ttgacttcgc caagctcaag tgaaatggaa aaactaaact
     5521 tgagtaaaga tgacacgatc ttgcgaatgg aacgtattcg tttcgcagat gatgtcccta
     5581 tttgttttga agttgccagt gtgccacaaa agctgatcga tggctacagc aaagcagaga
     5641 ttactgaatc gttgtatcgc acgctagaag aaaaaggcgg gaaaaagatc ggtccagcga
     5701 atcaaacgat tactgctgtg ttggcttctg agaaaattgc tgactattta gaggtaaaac
     5761 gaggagatgc agttcttcgt ttgcgccaga tttcttattt tgaagacggt acgccgtttg
     5821 agtatgtgcg tacgcaatac gtaggaaatc gttttgagtt ttatttggaa aaataaaaaa
     5881 caggtgatcc ctgattccgt attagaaata aaagagaatc agaatcacct gtttttatat
     5941 atgatcagtt accagttgct cgtgataaga aacaggaccc tcttcaaatt ggacttgtcc
     6001 gttcagcagc tcaatgatgg attctttaaa gtggtcagca cttttttcat ctacagcaca
     6061 gatgacgcta acttgatcag tatattggat ttcttttata aaaaaatgtt ggagttcaag
     6121 ataattctgt aattttccta gttgcggata cgtgatcatc acagagatct cctgttgcaa
     6181 ggtaccttct acgattccaa tgtcagacaa tgcttgagag accgcatgag tgtatgctcg
     6241 tattaatccg cctgtgccga gtttcgttcc tccaaaatat cgagtgacta ctgcagctac
     6301 attgatcagt tgattttttt tgaggacttc caacatcggt atgcccgccg ttccacttgg
     6361 ttctccatca tcgctgcttc gctggatttc atttttttct ccaatgatga aggcgctgca
     6421 gttatggttc gctttccaat gttctttttt tatttcttga atgaaacttt ttgcttcgtc
     6481 ctctgattct attcgtctaa gagaacagat aaagcgtgat tttttgatct cgatctcact
     6541 ttgtccattt tcttttattg tacgatagcg ctcgatcatc ttgtcacctc ttttttcatt
     6601 ttctttattc agtataaata aagttctagg ttgtttcaac actgtaacgg ttgcgcaatt
     6661 gctgtagtat gcgataataa agacaaaggc aggtggatct ggggatgcag gaacgaatga
     6721 aaaagtacga tgcaattacg cattatttga agaacaatgg cggctctcaa gtaacgttga
     6781 cctttaccca atttgacgaa ctgctgtttc ccagtaacgg acttcccaaa acagcaaggg
     6841 aaagtacaga ttggtgggca aatgattata aacaccctga aaaaggtgcc tatggctgga
     6901 taaatgcagg ctatgaagta gttgtcatca atttggacaa agaatatgtc gtgttcaata
     6961 aattagtgaa atctagttgg ctatttgatt aaataaaccg cgtttagaaa acgttgacaa
     7021 atttctttct ttttatataa tagagtaaca gacgcgaaga aaacgtttgc gtagtttgtt
     7081 ttaaaaagga gagaaaagta tggctaaaaa aacaatcatg cttgtatgtt cggctggtat
     7141 gagcacaagt ttactagtaa caaaaatgca aaaagctgct gaagcaaaag gaatcgactc
     7201 tgatattttc gctgtgtccg cttctgacgc agataacaat cttgcaaata aagacgtgga
     7261 tgtgttgtta ttaggcccac aagttcgctt tatgaaagct gattttgaga aacgtctgga
     7321 accaaaaggc attcctttag atgtcatcaa tatggctgat tatggtatga tgaatggcga
     7381 aaaagtgtta gatcaagcaa tcacgctcat aggtgaataa atagaacagt ataactatcg
     7441 aacaagcgat ccaaaaaata gatcgcttgt tttttcgtat atttttttag ggcgaataat
     7501 atgaatcatt tttggggtag aaggcttctg acgagagata ttaaagcaat agactgtgag
     7561 gaggtaaatt taccaaatta tgagaagatc gcagcagtgg aacgtttgag caactggatt
     7621 ctttgtcatc gctgtggaat gagaacgtcc gtcattgatg gacagcttgc agattacggt
     7681 tatttttgta cacattgtct atatttaggg cgatgcgaca ctcaacaaga tttatatttg
     7741 tttgatcagc ctgaagcaaa acctcgagaa gtcgtctttt tttggacagg acagcttact
     7801 gaaaaacaaa cggaaatctc aaagagaata ctttgccatt cagaaaaaag acatcatctt
     7861 atttgggctg taacaggtgc agggaaaaca gagatgcttt atcctatttt agtggaaact
     7921 ttaaaagctg gaggaagagt agcgatttgt acaccgcgag tcgatgtttg taatgaattg
     7981 tttttacgct atcgttatgt ttttccagaa gaaaaaatca cgttgctcca tgggaataca
     8041 gcaacggctt atacctttac ttcttttgtt atctgtacga ttcatcagct ataccgtttt
     8101 tatcggtcct ttgatttaat agtgatcgat gagatcgatg cgtttcctta taacggggat
     8161 gcaggattag ctcatgctgt gcaaacagcg attaaaccag acggacgatt catctactta
     8221 agtgccacac ctgataaaaa gttactaaag gagatcaagc agacgtttga catccacaaa
     8281 cttgctttac gctttcatcg gcgtttgctt cctgaacccg tcttggtttt catgaataat
     8341 tggcgacaaa aatgcaagaa caagaagaag ataaaatctt tggtgcggat cattcatcaa
     8401 ttaattaaag aaaaccatat ccttttattt tgtccaagta tcacgatgat gcatcgtttg
     8461 aaagccacac ttcaagaggc tcttccgaga tatgagatca cagatgtttc ttcaagagac
     8521 aatcagcggg cggaaaaagt ccagaaaatg cgagaagcaa aatatgacat actgttaacc
     8581 acgatgatcc ttgaacgtgg agtcacattt gaaagaatat ccgttatcgt tttaggagcg
     8641 gatcatccgg tttttactaa atcttctttg gtacaaatcg caggaagagc cgatcgtaaa
     8701 gggccttata cgaacagtca agtatatttt ttctatgaag aaaaaactgc agctattcga
     8761 aaagcttgtc aggaaatcaa agaaatgaat gaagaaggga agaagatact gtgaggtgca
     8821 gttgctgcag taaagatatc gtccaaaatt taacattcag cgagattatt ggtgtttcga
     8881 aatacagatt acctcagact tgttcagttt gtcgcgcaca atttcattta ttggaaacaa
     8941 gaggctgttc tggctgtcaa aaaccaatcc atttcaatct agagagtacg aaaaagaaag
     9001 taaattctat ccccgtttac tgcagtgact gtcaagaatg gaagaagaaa tatccgacct
     9061 atgattttca gcaccgtgcc ttttttgtgt acgatgaaaa tatgaaggcc tggctcaatc
     9121 aatacaagtt tatgggagat atccgactag caggcacctt tgcatataaa tggacgcaga
     9181 taaaaaaaga atacccgaca tacatctatt gtccaatacc tttatctgaa aaaaggcgaa
     9241 cagaaagagg attcaatcaa gtaagcgaga tgttgaaagc agcaagaatt ccttttcaga
     9301 tgctactcaa aagagagaag catctgctgg ctcaagcaaa aaacacgcga aaggaaagat
     9361 tggagatgcc tcaacctttt gaacttgcag tcaccttttc taagatacaa aataaaaaaa
     9421 tattgattat tgatgatgtc tatacaacag gacagactct tttccacgca gcagattgtt
     9481 tgcagatcgc acagccgaaa atcatccgaa ctatttcttt ggcgagatga attagatgtg
     9541 gaaatttgat agcgatttaa tttgcaaata acctagagtt cgatataata gaattaagaa
     9601 gagggaaaga gtggtccttc tccttcttga ttgtggtaga cgaaaggggt aatttttatg
     9661 tttagatata atgtacgcgg cgaaaatatc gaagtaactg aagcgattcg cgactacgtt
     9721 gagaaaaaag taggcaaact agaacgctat ttcagtgacg cacccgatgc aacggcgcat
     9781 gtgaacttaa aagtttatac agaaaaaaca gcaaaagtgg aagtaacgat cccattacct
     9841 tatcttgtat tacgtgccga agaaacttct ccagatttat acgcaagtat cgatttggtt
     9901 gttgacaaat tggaacgcca aatccgcaaa ttcaaaacaa aaattaatcg taaatcccgt
     9961 gaaacgggca tgaatactgc aaatgctgct gctttattca atgaagagga cgaaaacgaa
    10021 aatgattctg aattagatat cgtacgtacg aaacgccttt cattgaaacc aatggatagt
    10081 gaagaagcag tcttgcaaat gaacatgctg ggacataact tctttatttt tgaagatgca
    10141 gaaacaaatg gtacaagtat cgtttaccgc cgtaaagatg gaaaatacgg actgattgaa
    10201 acagactgat cagccttact taaataacaa cgaaaacttg cagcagctac tgacagctgc
    10261 cgcaagtttt tttgagtttc ctcctctttt agatgaaata tgaataaatt atgaagttct
    10321 tactttaata cgcttaagat atttcaatta tctataacta atgataaaat gaaaagtaaa
    10381 gtctacatag tctggataat acacagaagg aaaaggagat tacacaacgc atggccaatt
    10441 tgattcgttc acttatcgaa aacgataaga aagaactaaa acgtttagat agcattgcaa
    10501 aaaaagttga agcatacgct gaccaaatgg cagcattaac tgatgaacaa ttacaggcaa
    10561 aaactgctga attcaagggc agataccaaa aaggagaaac attagatcaa ttacttccgg
    10621 aagcttttgc agtcgtccga gaagcagcaa aacgtgtatt aggtttatat ccataccacg
    10681 tacaattgat gggtggaatc gtgcttcacg atggaaatat ccctgaaatg cgtactggtg
    10741 aaggtaaaac cttgaccgca acgatgccag tatatctaaa tgctctgact ggcgaagggg
    10801 tacacgtagt taccgtcaat gaatatttag caacccgtga ctcgacggaa atgggcgaac
    10861 tttataattt cttaggatta acagtggggt tgaatatcaa ctcaaaatct tcggatgaaa
    10921 aacgcgaggc ttataattgt gatatcactt atagtacgaa taatgaatta ggatttgact
    10981 acttgagaga caatatggtc gtttaccgcc atcaaatggt acaacgtccg ctgaattatg
    11041 ccattgtcga tgaagttgac tcaatcttga ttgatgaagc gagaacgccg ctgatcattt
    11101 cagggcaagc agaaaaatcc acagctttat atacaagagc agacaacttc gtgaaacgtt
    11161 tgaaagaaga agaagattat aaaatcgatg tacaatcaaa aacaatcagt ttaaccgaag
    11221 cagggatcga aaaagcagaa gaaacattcg gtttggataa cttatacgac attgaaaata
    11281 ctgcattgac ccatcacttg gatcaagcat tgagagccaa ctatatcatg cttttagata
    11341 tcgattatgt tgtccaagac ggaaaagtac tgattgttga ccagttcact ggtcggatca
    11401 tggatggacg ccgttattca gatggacttc accaagcaat cgaagctaaa gaaggcgtag
    11461 agatcgaaga cgaaacgaaa accatggcga cgatcacttt ccagaactat ttccgtatgt
    11521 acaagaaact tgcgggaatg actggtacag ctaaaacaga agaagaagaa ttcagagaaa
    11581 tttataacat ccaagtatac cagattccga caaatcgtcc ggttatccgt gatgatcgtc
    11641 cagatttgct ttacccaaca ttacaaagca agttccacgc tgtcgtgcaa gatatcaaag
    11701 atcgctaccg caaaggtcaa cctgtgttag tcggaactgt tgcggttgaa acatctgagt
    11761 tattatcaga tatgctgaat caagaacgta ttcctcatga agtgctgaat gcgaaaaatc
    11821 actttaaaga agcagagatc atcatgaatg ctggtcaaaa aggtgctgtg acgattgcta
    11881 ccaacatggc tggtcgggga acagatatca agttaggact tggggtaaga gaacttggtg
    11941 gtctagcagt tatcgggaca gaacgacatg aatctcgtcg tatcgataac cagcttcgag
    12001 gacgtgcagg acgtcaagga gaccctggta tgtcacagtt ctacttgtca ttggaagatg
    12061 atttgatgaa acgattcggt tcagaacgaa tcaaatcttt cttagatcgg atgaagatcg
    12121 gtgaggaaga tgctgttatc caaagcaaga tgctgacgaa acaagtagaa tctgcacaaa
    12181 aacgtgtaga aggaaataac tatgacacac gtaaaaacgt cttacaatac gatgatgtga
    12241 tgcgtgaaca acgtgaagta atctataaac agcgacaaga agtaatcatg gaagaaaaaa
    12301 gcttatccaa gccactaatg aacatggtca aacggacaat cagtcgtgta gtagatgcgc
    12361 atacacaatt agagaaagaa aattggaatt tggaaagtat catcgatttt gccggcagta
    12421 ctcttgtcca tgaagatagt attactcttg cagatatcga aggaaaaaca gctcaggaga
    12481 tcaaagagta tctaaacgaa cgagcacaag aagtatttaa taccaaagcg gctcaactaa
    12541 atggtccaga gcaactgttg gaatttgaaa aagtcgtgat cttgcgtgtt gtggactcta
    12601 aatggacaga ccatattgat gcaatggatc aattacgtca gtcaatcggc ttacgtgctt
    12661 atggacagaa caatccatta gtcgaatatc aaacagaagg ctataagatg tttgaagata
    12721 tggtaggcgc catcgagtat gaagtcactc gaatctttat gaaagctgag atccgtcaaa
    12781 acgtacagcg tgaacaggtt tctcaaggaa cagcttctca ctcagaagac ggagatgcca
    12841 aagaaaatag taatactagc gcgaaaaaac agccaatcca tgtgaacaag gtcggaagaa
    12901 atgatccttg tccttgtgga agcgggaaaa aatataagaa ctgtcatgga aaaaatgcaa
    12961 actaatttct gacagcagga ttggtgagac aaattctttt tttaagaatt cgtctcacct
    13021 tctttctgtt atttgctgat tctgttagac tagatataat gaaaaaagta tagtgaaaga
    13081 aggaaaaaac atggaaatat ccgaaattcg aaatctcctg acagaaatgc aggaaaaggt
    13141 cacaagcttc aggaggtctc tttgacttag accagctgga agaagacatt gcggaagcag
    13201 aagcaaaaat ggctgaacct ggtttttggg atgatagcga agctgctcaa aaagtgatca
    13261 gtgaaaacaa tgcaaataaa gaaacttatg atcaattcca ttctcttgct gaagaactgg
    13321 aagaaatcga attgatggtg gagatgttgc aggaagaacc tgatgcagcc atgcaagaag
    13381 aagcagaaac acgtatcaat gcgctagatg aaaaaatgaa gacatatgaa ttatctatgt
    13441 tgttagatgg tccttatgat cgaaacaacg ccatcgttga attgcatcca ggggctggcg
    13501 gaacggagtc ccaagattgg ggcagtatgc ttttacgtat gtatacacgc tgggcagagc
    13561 agcatgggta tcaagtagag acactagatt atcaagcagg tgatgaagca ggaatcaaga
    13621 gtgtgaccct actgatcaaa ggacacaacg catttggtta tctgaaatca gaaaaaggtg
    13681 ttcatcgcct tgtacgtatc tcgccttttg actcagcgaa acgacgtcat acttcttttt
    13741 gttcggtaga tgtgatgcca gagcttgatg aaacgatcga tatagaaatc aatccagatg
    13801 atttgaaaat cgatacgtac cgagcaagtg gtgcaggtgg tcagcacatc aataaaacag
    13861 aatctgctgt acggatcact cacttgccta caggaacagt agtagctagt caggcacaac
    13921 gttcacaatt gaaaaaccgt gagcaagcga tggggatgtt aaaagctaag ctttatcaat
    13981 tagaagtgga aaagaaagaa caagaagcag ctgcacttcg aggagaacaa aaagaaatcg
    14041 gctggggttc acagatccgt tcttatgttt tccatccata ttctatggta aaagaccatc
    14101 ggacaaacgt agagacagga aatgtccaag cagttatgga cggtgacctt gatttattta
    14161 ttgacggcta tttaaaaatg catctacaat aagaacccat tgttaaattg aaataaaatt
    14221 gtaagatttt gcaagcatgt tgaaacattg ccatgctata atagctaaag actgaaaaag
    14281 atatggagag aacgccatga ttgaaatgaa ggatgtaatg aagaagtact caaatggtac
    14341 gaccgctatc cgcaatctct caattgtcat cgaacaagga gagtttgtct atgtggttgg
    14401 tccgtctgga gccggtaaat ctacattcat caaattgatg tatcgagaag aaaaagcttc
    14461 aaaaggaact ttgaacgtcg caggtcatga tctaatagca atcaaaaacc gcgaagttcc
    14521 ttatctacgc agagaaatcg gcgtggtgtt ccaagactat aaattattac caagaaaaac
    14581 agtatatgaa aatgttgcgt acgctatgca agttatcggc agaaaacctc gggatatcaa
    14641 aaaacgagta atggaagtgc tggaccttgt tggtttgaaa cacaaagttc gagtctttcc
    14701 tagcgaatta tccggtgggg aacagcaacg tgtctctatt gctcgggcaa tagtgaatac
    14761 gcctaaagta cttatcgcgg atgaaccgac aggaaatctg gatccagaaa actcatggga
    14821 aatcatgaaa ttattagatc ggatcaatgc ccaaggaact actgtcgtga tggctacaca
    14881 taacagtacg atcgtaaata cgattcggca ccgagtaatt gctatcgaaa acggccgcat
    14941 tattcgagat caagcggaag gggaatatgg atacgatgat tagaaccttt ttccgccatt
    15001 tattagaaag tatcaagagt ttgaaacgta acggctggat gacagttgct tcagtcagtg
    15061 cggtaacgat cacattgact ttagtcggtg tattcatggc agttatcatg aacgcaacta
    15121 aattagctca agatatagaa ggaaacgttg atgtctctgt attcgtagat atcggtacaa
    15181 aagacgaaga gatgaaaaag ctagaaaaag aattgaatga cttagatcat gttaaaaaag
    15241 ttgaattttc aagcaaagaa caggaattga aaaaaatcca aaacgaaatg ggtgattcct
    15301 ggaaattgtt tgaaggtgac aataacccgt tatatgatgt gtatgtagta agtgctactg
    15361 atccttctta tacaaaacaa gtagcagaag aagcagcaga tttgaagaat gtttctcgtt
    15421 ctgattacgg tggagcttcc tcagatcgga ttttccaaat cgctggtgca gttcggacat
    15481 ggggattagt tgcagcggca ttgctgctgt tcgtagcgat gtttctcatt tcgaacacga
    15541 ttcggatcac gattttgtct agacagcgtg aaattcaaat catgcgttta gtcggtgcaa
    15601 aaaatggata tattcgctgg ccgtttttcc ttgaaggtgg ctggatcggt ctgatcggtg
    15661 cgattcttcc gattttgatc atcacatttg gttatgcatg gtttttccaa ttgatcaatc
    15721 catcattatt acgttcccat tattcattga ttcatccaca aaatatcgtt tggaaaatca
    15781 atcttttaat ggtcggcatt ggtgtgatca ttggttcact tggctcgatc atttctatga
    15841 gacgtttctt gaaattctaa taccacaata taaataaaat gagaaagttg gacagtttcg
    15901 ttccagcttt ctttttttat tggctaggag aatagaagga agcggctttt ttccctttac
    15961 tttaaaaaac aacttgggta ttcttaagtt ggtagaagaa gaggtgaagc cgtgaaaatt
    16021 atcgttgaca atttttttac gatccttttg attatgaata ttttgctttc ctttatcatc
    16081 gtttttcgag aaagaaagga aactgctcaa acatgggcat gggtgctcgt tttgatgttc
    16141 attccagttg taggatttat tttatatatt tttttaggaa gagggatttc caaagagaaa
    16201 atctttgatt tgaagatcca agggaaaatc ggtaagaacc tggaaattga agaagacaaa
    16261 caggcattga tgcgagggct ttatcctcat ccgcctactg gacaagtaga tgtgaaacaa
    16321 ttgatctata tgctcacggt atttgaaagt acgctctata cgactaaaaa tgaaatcact
    16381 ttatttacag atggtcgtga aaaattcgat gcgctgattg aagatatcaa gcaggcagaa
    16441 gaccatatcc acttccagta ttatatttat cgcagtgatg cattaggtga agaagttcga
    16501 gatgcattga cagaggcggc aagacgaggg gtaaaagtca gagtccttct tgatgcatgg
    16561 ggttccacac aagtttcttc cagctttttt cagaatttga aaaaagcagg cggagaagtc
    16621 gcatttttct ttcctctatt tgttccgtat attaatcctc ggatcaatta tcgcaatcat
    16681 cgtaaaatcg tagttattga tgggaaaatc ggatatacag gtgggttcaa tgttggaaat
    16741 gaatatctag gattggtaaa gaaatttggt tattggcgag acaatcattt gcgtatttac
    16801 ggagaagcgg tgtatacctt gcaaaaccgt tttttgatgg actggaattc tcagcataca
    16861 aaagaagtag gctattctcc caaatttttt ccgtccattc actctactgg tgatatagct
    16921 gttcaaatcg tgacaagcgg accagatacc gaacatgaac aaatcaaaat gacttatttg
    16981 aaaatgattt cgttagccaa acgggagata ttgatccaaa cgccttatta cataccggat
    17041 ggatcgattc atgaagcact gaagttggct cttctttcag gcgtaaaagt tcatatccaa
    17101 atacctaaca aaccagatca tcttctcgtt tactgggcaa cgtattcttt cgctgcagag
    17161 ctaatcgagt atggagcaag gatcgaaaca tatgagaatg gatttattca tgcaaagacg
    17221 atgattatcg atggtggcat tgtttcagtt ggctcagcaa atatcgatgt tcgttcattt
    17281 cgcttggact ttgaggtcaa tacactggtt tatgatgaac gaatggcttc acgtgtaaga
    17341 aaagcttttt ttgaagactc gaaaatttct acgcatctca caaaagaaat gtatgaaaac
    17401 cgaggaataa tcatcaagat gaaagaagga ttagcaagat tgatttcgcc gctcctttaa
    17461 aacataaaaa ggacatgaga accatcaaca gatggagatc tcgtgtcctt ttctaacggg
    17521 gccatttctg agagtacgtc agaaatggag aaagcgtctc gcatgagcga aattaacttt
    17581 ggggtatctc ttgttttttg gggaaagcaa gaaataagct aatatatgct attaaaagag
    17641 acacatttca aagccagtat agcatagaaa cttttaatga gatagttttt tttacaatta
    17701 tgagaattta ttttgttatt taatgatttt gattcgtttt catcaagtaa tgaaaatgat
    17761 aaaataaaca tactttagga ttaaaggaga tttctaatga aaaaacttaa attattctta
    17821 ccaataatag gattagctat tcttttatca ggctgttcaa agtggattga tcgaggagaa
    17881 tccattacgg ctgttggctc ttctgcttta cagcctttag tagaaacagt tgctgaaact
    17941 taccagacag aaaatcctgg aaaattcatc aatgtacaag ggggcggaag cggaactggt
    18001 ttgtctcaag tacagtcagg agcagtcgac atcggaaact ctgatttgtt tgcggaagaa
    18061 aaatcgggga tcgatgcctc agcattagtc gatcatcgag tagcagttgt tgggatcact
    18121 cctattgtga acaaggatat tggcgtaaaa gacatcagta tggaaaacct gaaaaaacta
    18181 tttacaggcg agatcaaaaa ctggagtgaa gtcggtggaa aagatcagcc agtagttata
    18241 ttgaaccgcg cttcaggaag cggaacccgt agcacgtttg aaaaatgggt attggacgga
    18301 gaaacagctg ttcaggcaca agaacaagat tcaagcggaa tggtgcgcca gatcgttgcc
    18361 gacacacccg gtgcaatcag ctatgttgct ttttcttatg tttccgatga tgtagatacg
    18421 ttgaatgtta acggtgtaga accgacagat gaaaatgtga caacgaacaa atggacgatt
    18481 tgggcatatg agcatatgta cacaagagat catccaaaaa aactgacaaa agaattttta
    18541 gactatatct tatctgatga agtacaagat aacatcgttg gagaactagg ttatatccct
    18601 gtttctcata tgaaagtcga acgtgattgg caaggaaata tcgttaaaga gtaatcttta
    18661 cacaaaattt acaaacttag ctaaactttt acatgttatt aaaaccaaat tgatgttcaa
    18721 tttgtacact gagtgagtat gttaaacctg attaaggggg agtcatctgt ggaagatgtg
    18781 aaaaaagtat tattaacaaa atcaaaacgt tcaaaaatgg aacagcgtgg aaaattcatc
    18841 agttttttat gtatcgcgct gatcgtgttg gttgtattat ctatttttta ttttgtagct
    18901 agtaaaggat tggccacctt ttttactgat aagatcaatg tatttgattt tctgtttggt
    18961 acagattgga atccaagcca aatcggagca aatgggaaac caatggtagg ggcattgcct
    19021 atgatcacag gttcgtttat tgtgaccttt ttatctgcta tcgttgccac gccatttgcc
    19081 attggtgcag cagtattcat gacggagatc tctcctaaaa acggaacgaa gatcttacag
    19141 ccggtcatcg agctgcttgt aggtattcct tctgtcgttt atggttttat cggtttatca
    19201 gttatcgtgc cttttgtcag aagtattttt ggcgggactg ggtttggtat cttagctggt
    19261 acatttgtgt tgttcgttat gatcttgccg actatcacaa cgatgactgt tgatgcatta
    19321 aaggcagtgc cacggcacta tcgggaagct tcattagcgc ttggtgccac aagatggcaa
    19381 acaatctaca aagtcgtgct acgagcagcc gtgcccggga ttttaacagc agtcgttttt
    19441 ggtatggctc gtgcgtttgg tgaagcctta gctatccaaa tggtcatcgg taatgcagca
    19501 ttgatgccaa caggtcttac tactcctgct tctacattga cgtccatctt aacaatggga
    19561 atcggaaata caatcatggg aacagttgaa aataatgtgt tatggtcatt agcgctgatc
    19621 ttgctattga tgtcattatt ctttaatatc gtgatccgta tgattgggaa gaaaggagag
    19681 ctgaaataat gaacgcaaaa cgctcagata aaattgctac aggtgtttta tatatagttt
    19741 cggggatcat cgtattgatt ttagcagctt tactacttta tatcttagtt cggggaatac
    19801 cgcacatttc atggtccttc ttgaccgaac cttccaaagc ctaccaagaa ggcggtggaa
    19861 tcgggatcca gctatttaac tcgatttatt tgcttttgat cacgatgatc atcagttttc
    19921 ctatttcttt aggtgcgggt atctatcttt cagaatatgc tggaaaaaac tggctaacag
    19981 atgtgattcg cacgtcgatt gaaatcttga gctcgttgcc ttctgtcgtt gtcggtttat
    20041 tcggtttctt ggttttcgtt attcaattcc agtatggttt ctcgattatt tctggtgcat
    20101 tggctttgac tgtattcaac ttgcctcttt tgactagaac agttgaggag tctttacaag
    20161 cggtccatta cacacaacga gaagcaggat tggccttagg tttgtcacgc tgggaaacag
    20221 tgataaaagt ggtggttcca gaagcgacac cagggatttt gactggtgtg atcctaagtt
    20281 ctggaagaat ttttggtgaa gcagctgcat tgatctatac ggctggacaa agtgcacctg
    20341 cgttagattt tacgaactgg aatccattaa gtgtttcaag cccgatcagc attttccgtc
    20401 aagctgaaac attagctgtc catatttgga agatcaatac agaaggcacg atgcctgatg
    20461 gtgtttctgt ttcggctggc gcttctgccg tgttgattat tgctgtttta ctgttcaact
    20521 tcggcgctcg tgcgattggc aaacgattat accgaaaaat gacttcagcc taaggagaag
    20581 aagcaaaatg aaaaaatata actggaatga aacaaatgtc atcaccatgc caaaagagaa
    20641 agaagtagca ctatatacgg atgatctaca tgtctggtat ggcgacaatg aagcaatcaa
    20701 aggagtggat ctggaatttg aaaaaaataa gatcactgca ctgattggtc cttccggctg
    20761 cggaaaatct acgtatcttc gttcgttgaa tcgaatgaat gatgggatcg ctaatacgaa
    20821 aatcactggg aaaatcatgt atcaaggcgt agatattaat gcgcctcaag tggatgttta
    20881 tgagatgcgc aaacggattg gcatggtttt tcaacgtcct aatccgttca gcaagtcgat
    20941 ttatgacaat attacatttg ctttgaaaca gcacggcgaa aaaaacaaaa agaaattaga
    21001 tgaaatcgtt gaaacaagct tgaaacaagc tgccttgtgg gaccaagtaa aagacagctt
    21061 ggacaaaagt gccttggctt tatctggcgg gcaacagcaa cgtttatgta ttgctcgtgc
    21121 gatcgcgatg aaacctgata tcttgctgct agacgagccg gcaagtgctt tggaccctat
    21181 ttcgacagga acggttgaag aaacacttgt aaacttgaaa gaagactata cgatcatcat
    21241 cgtgacgcat aacatgcaac aagcagctcg tatcagcgac tatactgcct ttttctattt
    21301 aggaaaagta atggaatatg acaaaactaa caagatattt accagaccaa aaattcaggc
    21361 aaccgaagat tatgtgtccg gtcattttgg ttaagacgga ggcctcaaca tgacaaaaga
    21421 gattatttca tctaaagatc ttcatctgta ttatggaaag aaagaagcac taaaagggat
    21481 cgatctaagc ttcaatcaag gggaaatcac tgcgatgatc ggtccttctg gttgcggaaa
    21541 atccacttat ctgcgttctt tgaaccggat gaatgacctg attccgaatg taacgatcac
    21601 cggaagtgtt ttatataaag acaaagacat ctatggtcca aaaacagatg ttgttgaact
    21661 aagaaaagaa atcggcatgg tattccagca gccaaaccca tttccttttt ctatctatga
    21721 aaatgtcatc tatggattga aattaaaagg ggaaaaagat aaaaaagtcc ttgatcaagt
    21781 agttgaagaa agcctgaagg cagcgtctgt ttgggatgat gtgaaagaca aattgcataa
    21841 aagtgcttta tctttatccg gtgggcagca gcagcgtgta tgtatcgcac gtgttttggc
    21901 agttaatccg gaaatcatct tattggatga accaacaagt gcgcttgatc ctgtttctac
    21961 tgggaaaatc gaaagtatgc tgttagaatt aagagagcgg tatacgatga tcatcgtgac
    22021 ccataatatg tctcaagctt cacgtatttc agataagacg gcattctttt tagatggtca
    22081 cttgatcgaa tacaatgata cgaagaagat attcatgaat ccagaaaaac aagagacaga
    22141 agattatatt tcaggtcgat ttggataagg aggaaacgga atgctacgta cacaatttga
    22201 agaagaattg ctgaatctcc ataatcagtt ctacgaaatg gggatgatgg tgagcagcgc
    22261 cgtgcacaag tcagttcgtg catacatcaa acacgataaa atcttagccc aagaagttat
    22321 cgaaaacgat ataaatatca ataacatgga aacacgcttg gagaagaaaa gttttgaaat
    22381 gatcgcattg caacagccag ttacaactga tttgcgtatg atcattacgg tcatgaaagc
    22441 tagctcggat ttagaacgta tggccgatca tgcggtgtct gttgctaaat ccacgattcg
    22501 cgtaaaaggg cagacgagaa taccggaaat cgaaaaagag atctccgata tgtccgatta
    22561 tgtgaaaaaa atggtggaca acgtcctagt tgcttatgtc aaaacagacg aaaaagacgc
    22621 acgtacgatc gccaatatgg atcaaagagt caatgactat ttcaatcgaa tctataatgc
    22681 aacaatcaaa aatatgcagg aaaatcctga gacagtcatc agtggaacag actacttgaa
    22741 tgttgccagt tatctagaac ggatcggaga ctatgtgaca aacatctgtg aatggatcgt
    22801 ctacttggct acagggaaaa tcactgagtt aaatacgaac cacaatgaga ttttctaaac
    22861 tgaagctgtg attatgccgt ttgtttagga aaagaggttg tgaaatcagc ggttgtgaaa
    22921 aagctgttct gcatcaagta ataagaacag tcaaacaaaa aatagctcct catatttttc
    22981 gtttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt cgggaaaggg
    23041 tttgtgacaa aacatgtcac aaaccctttt catcatttca tagaaaggat aaagaatgca
    23101 aaaaaaaatt agcatcatca ctatattttt tatttgtgtg cttacttcat gggttattca
    23161 gcatcaagcc gctttatatg aagtacctgt catcgagata gaaaaagtac gaataaaaga
    23221 ggatcatcaa ttgattacag gaaaactgat gaacacggat caaaaaggag aatcgattta
    23281 tttatcggtt ccttatcaaa aaaatcaaat agaaacagca aatataaaag cgagacaaat
    23341 gtatctagtc caaatgcatg gagatgaatg gcagctagta gcaaaaaaaa atgatggctg
    23401 gcttttcttc ttcacaagta tcttcattgg cgccatgttg cttattggtg gaaaacaggg
    23461 tctatttgct ttaggaagtc tcatcatcaa tatcgtttta cttttacttt tgttgctcat
    23521 ctttactaaa acagaaggga tttctctatt aaatatatct atcctgttcg tcgttatagg
    23581 gatgattatt agcatattgg ctttagatgg atggaataga agcagtataa tcaaaatttc
    23641 agctgcagtt acgagtgttt ttttagcatt ttttatttgt cttttgacaa tgaatcattg
    23701 gaaagaccaa ggactgcgct atgaggaact gaattatctg acacgaccgt atcgttctgt
    23761 ttttctttct agcgtactga ttggaacagc tggaggaaca ctcgataccg ttatcacagt
    23821 cattgcgaca ttagaagaat tgactaagcg acaaccagaa ataacagcga aacagttatg
    23881 gctttcagga aggcaagtgg gtcgagatgt gattggctcg atgaccaata tccttttgtt
    23941 cgtctatatc agtgggtcag ttccatcgct tcttttatat ttaggaaatc aatggtcgtt
    24001 caaagaaacg atagaacagc atttatcatt ggaattttta cgcgtcattg ctggcagctt
    24061 aggaatagta ttatcgatcc ctatctcggt cctctttttt ctgactgtaa ggaggctgaa
    24121 acgatgaatg tacttattat acttgttggg attttagcaa tcagcgtgct acttattggc
    24181 ggtacgcaag gtatgtatat tcttttaggc ttattcatca atttagggat ctttttcctg
    24241 ttgttattcg gttatcatca gaagtggccg atactggcat tgtcggtcat aggattttta
    24301 ttgattgctg tggttatctt attttttatc aacggttata atctaaaaat gcgtgcagct
    24361 tttgtgagta tactgatctt tttattttgt ttcctactat tgcttcctat cactgatttt
    24421 ttagcaatcc aaggttttac atcggtagaa ctagaagaat tatctggctt agataagact
    24481 ttagcgattg attttagatt acttacgaga tcgttgcttc tgattagtct tagtggtgct
    24541 gttttagatg cgagtgtagc aatttctagt gggacattcg aagtatatca ggcaaatccg
    24601 caattatctt tttaccaatt gcgccattct agtttcgcta ttgctaaaaa aatattggcg
    24661 tcaacagtaa tgacacttct ttttgcattc atgggaagtt ctttagcgtt gatcctctgg
    24721 tttattgatt tagacattcc ttttcagcaa gtgatcaatg aaaaatcatt tgttctagaa
    24781 tatacgatgg ctattcttac cacattgtcc gcactacttg ttcttccttt aacctgcgtg
    24841 actacagcat atctctttac aaaaaagaaa gaacaactca agatggagta aaagttacaa
    24901 cttaagtcct atgacatttt tactgaatca gtgcataata tggatgtaag gaaaagcttg
    24961 caaacaattt tgcttaagga ggcaatttcc atgaaagaaa gagaacgtat tttagattta
    25021 gtgaaaaaag gaatcctttc gactgaagaa ggcttagatt tattagaaag catggcaact
    25081 gaaaaagacg aaaagcaaat caaaaaagaa gcagacaagg taactgctag tcataaagaa
    25141 aaagatcagg caagtcaatt gatcgataaa cttgaaaacg gcgaagaaga aatttctgag
    25201 ccagttgatc caaaggaaag agaaagacaa gatcaagaaa acttagaaaa gatcttagat
    25261 gaactagcaa ctgaagcgaa taaaacttcg gcgcgtctag atgaagtaaa tgctcaattc
    25321 gctgacaata aggcagcacg caatgagaaa caggaagcat tgatgcagct gaatacaaaa
    25381 gaagaattag gcgagttgac agaagaagaa ttagcacagc gtcagacgtt agaagcagaa
    25441 atcaaagaat tagaagctag tggcgataca ctgttagaag aacaattgaa attagaagca
    25501 gaattaaaag atatccgtaa gaatcaatgg tctgagaaaa aagaaacgtt tactgataag
    25561 tttgaattgc cagatgattg gaaagatcaa gcaacagata cactgaatca agtaggcgaa
    25621 aaaatgtcag aagctggatc acagttaggc aaattcttga agaaaacatt ccaaactgtt
    25681 tcagaaacag tgaatgacaa catggaatgg aaagatgtca gcttacgtgt ccctgggatt
    25741 gcgacaacga aatttgagca tgaattttac tatgaagctc ctgcagcaag tatccttgat
    25801 atcaaggcag caaacggaaa cgtgacgtta aaaacgtggg acagcaacga tgtgaaagtt
    25861 gaagctaaga tcaaacttta cggaaaaatg ggtgctgaac catttgaagc attctctgaa
    25921 cggagccaga ttgaggttaa tgaagatcat atctccttcc aaattccaaa taaacgtgtg
    25981 cgtgcagacc ttgttttcta cttacctaaa cgagtatacg accacgcagc tattaaactt
    26041 ctaaatggca acatcatgat tgaaacattg gaagctaaag atatttatac aaaatctacg
    26101 aatggtaaca tcatcgtgga ccaattaact gcaacgatgc tggaagtaga aggtgtaaac
    26161 ggcaatatcg atattcgaaa cgggaacatc ttagattcta ttatcgaaac agtaaatggt
    26221 acagtgacat ttggtgcgac agcagaaaac ctttctgtgt ctttggtaaa tggtgatgta
    26281 cgtcttacga ttaaagaaga taatttgaaa aaagtagaag caagttcagt gaacggaaat
    26341 gtcaaagtcg ctttaccaga tggcatcggt ttggaaggac atgctaaaac aagtcttgga
    26401 agtatcaaca gccgtctgtc caattacgaa gtagttcgtg agaaaaaaga acggacaaac
    26461 caaatgttgc aattccgtcg tgtttcagat ggtgaaatcg cgcaagtgca actttcaacg
    26521 acaacaggaa gtatctattt aaaagataca gataaataaa gaggttgtga aagagatgtt
    26581 cagccttgag caataagaag cgattttgta aaatagcttc ccctatttta caaaattgtg
    26641 ccttattgcc gatagtctat ctcttgaaca ccgtttacca agagattatg aacctgccaa
    26701 ttatcgccga gtcacaagga ataatttcag aaaaagctcg tcacgtttta tgaatatggt
    26761 aatttgtgac cgagaatttt gcagaaagac accgattaag aagaaggttg tcacaacgat
    26821 tttcttcatt taaaatagta gaacagaggt gagacatgat gaaaaaaaag ttgacaaaat
    26881 ctaataaaaa tgttgttttg acagggactt tagcaggaat aggagaatac atcgggattg
    26941 atccgactgt tatccgtgtg ctttatgtat tcctgagctt agttgctttt ggaagtccaa
    27001 ttattttata cattttatta gctttgatta tcccatttgc gcgttctgca cgaacaaact
    27061 atggacataa caatgcatat tatgaaaaaa acaattatcg tgaacaaact cgtgaacaaa
    27121 caaaaaaacg caaagaagcg gaaaaaatcg atgatgacga atggagtgac ttttaatgtc
    27181 gtactttcaa cgtattttag ttaatacgtt aacattcata tcattggctg ttctttttcc
    27241 tgcgatggtc catgtgagca gtatttggac ggccgttctt gccagcttcg tattgtctgt
    27301 gttgaatatg tttgtcaaac cgattttgac gatactgtcc ctgcccttta cattagtcac
    27361 attgggattg tttacgtttg ttatcaatgc agcgattctt aaaatgacct ccttttttgt
    27421 gggagcagaa cattttagtt tttcttcatt cggtgcagcg ttgttagtag ctatcttgat
    27481 gtcgcttgtc aatgtaattg tcagcgaaca taacagtaga aaataaaaga aagctgtgcc
    27541 gaactatcgg cgcagttttt ttatttagaa tcaggaatgc gttttctatt acattggtaa
    27601 ttaaataaaa acgttttttt taattggtag tacttttggc tagacaatga taaaatgata
    27661 gagaacactc taatagaaat gaggtagcat gaatgtcaga agttgtaaaa gttagccaat
    27721 tagtgaaaaa attatctttg gaaatcgtga caggcgacga gaaaagctta gaccgtgtca
    27781 tcgtaactgg ggacatctca cgcccaggac tagagttgac tggttatttt aattattatt
    27841 cccataatcg tttacagcta tttggcagta aagaaatcag ttttgcggaa cggatgatcc
    27901 cagaagagcg tttgatcatc atgagaagat tatgtagtga agatatgccc gcatttatta
    27961 tttcaagagg attaccagct ccagaagaaa tgatccaggc agcaaataaa aatgggatag
    28021 ctgtactgcg ttctcccatc tcaacttctc gtttattagg agaattatcc agttatttag
    28081 atggtcgcct tgcccctcga acaagtgtcc acggtgtttt agtggatgtt tacggattag
    28141 gtgtactgat tcaaggtgac agtgggatag gaaaaagtga aacagcgttg gagttgatca
    28201 aacgcggcca tcgattgatc gcagacgatc gagtagatgt ttatcagcaa gatgaattaa
    28261 cggtgatcgg agaaccgcct aaaatcctgg aacatctgat tgaaatccga ggaatcggga
    28321 tcattgatgt aatgaatctt tttggtgcga gtgcggtacg tggctctatg caagtccagt
    28381 tggctgttta tctggaagca tgggcaaaag ataagaaata cgaccgtctg ggctctgaag
    28441 acacgaccgt tgaaatcgcg gaagtgggca ttccacaaat aaaaatccct gtcaaaacag
    28501 gacgaaacgt tgcaatcatc attgaagtag ccgcgatgaa tttccgtgca cgtacaatgg
    28561 gctttgacgc aacgaaaaca tttgaagaaa gattaagtcg cttgattgaa gaaaactcag
    28621 gcaacgacta gcaaggagtg acaatatgtt agcacaaatc aatcgagttg cttttgagct
    28681 tgtagggatt cctatctact ggtatgcatt gatcatcgta tcaggtatca ttatcgctat
    28741 gtggcttagc agcagagagg ctgttcgggt aggattaaaa gcagatgatg tgacagattt
    28801 tatgttgtgg ggattgccta tctccattat tggcgctcgc ttatattatg ttttatttga
    28861 tcttccccag tatatagctg atccaatcca aatattcaat atccgttcag gaggcttagc
    28921 tatttatggc ggactgattg caggcgcgat cacgctgtat ttttttacta aatatcattt
    28981 tatttctttg tggacttttt tagatatcgc tgcaccaagt gtattattag cacaggcaat
    29041 cggtcgttgg ggaaatttca tgaaccacga agcatatgga ccagacacga cgcgaagctt
    29101 cttagaaagt ctgcatttac cgcaattcat cattgataat atgtatatcg aagggatcta
    29161 tcgtcaacca acatttcttt atgaatcact ttggagtttg attggattta ttttgctttt
    29221 gtttttacga aagaaaccac accttttgaa aaaaggcgaa gtccttttat gttatctaat
    29281 gtggtatagt ttcgggcgtt tctttattga aggattacgt acagacagtc tttacttttt
    29341 aggtgtgatc cgtatttctc aattattatc tgctgtgtta tttgtcggta cattgattct
    29401 atttatttgg cgcagaaaaa aaggaaattt accggattat gaccgctcat taggaaaaaa
    29461 tcaacaaatt atctgatttt tagagcgtta aaaatgaaaa aaatttcagg agtggtaaaa
    29521 tgaattatag agtagcggtt ttaggaccag gctcttgggg tactgctttg gcacaagtcc
    29581 tcgccgaaaa cggacatgac gttcggattt ggggacatcg tgcttcacaa gtagaagaaa
    29641 tcaacaccca acacacgaat caacgttatt taccagatca ttatctaccc gattccatcc
    29701 aagcattcac caatatggaa gatgcagtaa aagacgcaga tgcggtgcta ttcgtcattc
    29761 caacgaaagc aatacgttct gttgcaaaag aactagtgcc taaattgaat acaaagccag
    29821 tgatcatcca tgcaagtaaa ggattagaac agggaacaca taaaaggatc tctgacatct
    29881 tgttagagga aatcccagca gaaaaaagaa aagctgttgt tgttctttct ggaccaagcc
    29941 atgcagaaga agtagcaaaa catgacatca ctacgatcac agctgcaagt acagatgcaa
    30001 aagcagcaaa atatgtacaa gagctgttca tgaataatta ttttcggatc tataccaatc
    30061 ctgatgtgat cggtgtagag atgggggcag ctttgaaaaa tattattgct attggtgctg
    30121 gcgctattca cggcttaggg tttggcgata atgcaaaggc agcaatcatg acgcgcggat
    30181 tagctgagat cagccgttta ggtgtagcaa tgggtgccaa cccgctgacc tttatcggat
    30241 taagcggaat aggcgatcta gtcgttactt gcacaagtgt tcattctagg aactggcgcg
    30301 caggcaactt gttaggacaa gggcagcccc tagaagaggt gctagccaat atgggaatgg
    30361 tcgttgaagg tgtgaatact acccaagcag cgatggaact agcccaaatg atggaaatcg
    30421 aaatgcccat cactcaaaca atctatgaag tgctgtatga acaaaaagaa atcaaaacag
    30481 ccgcaaaaga aatcatgtta cgtgatggaa aaaaagaaaa tgaattctca ttaacaattt
    30541 tttgaaggag cgaatgtata tgaaagttaa aaaagcagtt attccagcag caggattagg
    30601 aacaagattt ttaccagcaa caaaagctat ggcaaaggaa atgttgccaa ttgttgataa
    30661 accaacaatc cagttcatcg ttgaagaagc gttgaaatca ggtatcgaag acatcttgat
    30721 cgttacagga aaagctaaac gaccaatcga agatcacttt gattcaaata ttgaattaga
    30781 aacaaaccta agtgaaaaag gaaagaccga attattgaaa ttagtagaag aaacgacaga
    30841 tgtcaacttg catttcatcc gtcaatctca tccaaagggt ttaggacatg ccgttcttca
    30901 agcaagagcc tttattggaa atgaaccatt tgtcgttatg ctaggcgatg atataatgga
    30961 agacgaagta ccattgacac agcaattaat cgatgattac gaacgtactc atgcttctac
    31021 gatcgccgtt atgcaagttc ctcatgatga aacttcaaaa tacggaatta tcaatccagg
    31081 agaagtattg gaagatgggt tatacaatgt caaaaacttt gttgaaaaac cagatccaag
    31141 caaagcacca agtgatttag ctattattgg acgttactta ttgactccag aaatttttga
    31201 tgtattgtcc gagcaacaac caggagccgg aaatgaaatc caactaactg acgcaatcga
    31261 tacattgaat aaaacacaac gcgtatttgc ccgtaaattc acaggtaaac gttacgatgt
    31321 cggtgacaaa tttggtttta tgaaaacaag tattgaatat ggtctaacac atccagaagt
    31381 aggtgcacct ttacgtgagt acatcctgtc tttaggtaaa acattagaag cagaaggaag
    31441 caagatcgat cataatccaa aaggaataac agaaatcaaa gaagaaaaga aaaaataacc
    31501 cttttttcta acgagcttca gctgctaatt gaataaaaag tagttgaagc tcgtttttat
    31561 ttgtttaaaa aacacgaaaa tgctatcctt atagaaaaga gagacaagaa gggagtttga
    31621 tcatgtcagg aggagaaatt gcaggattga tcgcagcaat cgcctttgcg atacttgtcg
    31681 tttttatcgt tcgtgtgttg ttgcaagttt ctaaaacttt agaaaaagta gatcagactg
    31741 tagcagaagc aaatacgacg atcgaaatcg tcacaaaaga cgtagatctt ttgtctagac
    31801 aagtagaagg cttactggtc aaaagtaatg aactgctctt ggatatcaat ggaaaagtcg
    31861 caacgatcga tccattgttc acagcagttg ctgatttaag tgaaagtgta tcagaactta
    31921 atcattctag taaaaacatc gcgacgaaag tcggcggaat cggtaaaacg acagctaaag
    31981 cgacggttgc cggaaaagtc ggtgggacag cgatgaaatt ttttaaaaac aaaaaacata
    32041 ctgaaacgac aggaggaaaa taaaaatggc taaaaaaggt ggatttttat taggtgcagt
    32101 agtcggaggc acagccgcag caatcgccgc gttattgtta gcaccaaaat ctggtaaaga
    32161 acttcgggat gaattagctg cccaagcgga tgatttgatg gatatggctt cagattacac
    32221 agatatggcg aaagaaaaat cgactgagtt aacaggtatt gcaaaagaaa aagcgcaaga
    32281 tctttctcaa caagcaggtg atctagcagg tagtgtcaaa caaaaagtaa gtgaatcagc
    32341 agatgaagca ggaagtgaat ctgacgatat cttgttaaat cttaaagaac aatcagctga
    32401 cttatctaat cgtttcaaaa aaacggcaga agatatgaaa aaacctgcag atgaattagg
    32461 aagtattgct aaagatactt cagaagatat catgattgat gtaaaagacg ctgctggtaa
    32521 agcaggagat actgtcaatg aaggaatgtc agaagcaaaa ccagttgcag atgactcaaa
    32581 agaaatgatg aaaatggcaa aagaagatgc gaaagaagct aaaaaagaaa taaaagatac
    32641 aatgacagat tcatctacaa atcactaaaa tcacaaattt atagaataaa aaggtgggag
    32701 cggactagct ctcacctttt ttactcttat ttttctaaaa caagtaattc ttttggtgta
    32761 gtcgtgaatg gcacacagcc atcgcttgtc acatatacac agtcttcgat acgtacccct
    32821 actttttcag gaatatagat tcctggttca agagagaagc acatcccttc ttcgattact
    32881 aagtcatttc cttctacaag ggaagggaat tcgtggacgg ttgttccgat cccgtgacct
    32941 aaacggtgat tgaaatattc gccgtagcca tagctgttga tcacgtttcg tgcgatttga
    33001 tcaagttcac ctgctgtcac acctggacgg acggcttctt gtgcggcaag ctgtgcttca
    33061 agagtgatgt tgtagatttt ttcttggaag tcggttggtt tttgataagc aattgtacga
    33121 gtagcatcac tgcaatagcc attccaaaca acgccaagat cgaataacac aagttcattc
    33181 ggtgaaatct tcgtatttcc tggtgtacca tgagggcttg cagcatttgc tcctgcaagg
    33241 actaatgtgt cgaaagacat ttgtgaaaca ccttgttttt tcaattggta ttcaatttct
    33301 gcaatgatcg cttgttcagt cacgccttct ttcactgctt taaagccaat ctcaaatgca
    33361 acatcggccc atttaccagc ttcgatcagt ttgtcttttt cctctagagt tttgatcagt
    33421 tgtagacgtt ggatgatgga agttagatca gtgtaagaat ccgttcccgt caagatatca
    33481 gagatagcat cgaagcgctc gacagtcaag gcgttctttt caatccctac attgccaggc
    33541 tgaccatagc gagcacgaag ttcttttccg atgatctccc aagggttttc gctgtctaaa
    33601 tagccacgga catcatacgc ccaactactg ttttgagcat cttcaacttc caaagcagga
    33661 gtgaaaagga aaggatcttg ttcaagaggg ataaaaagag ctagtacacg ctcatgagga
    33721 tcactttcgt atccggaaaa gtaagcgatg tggccagggt cgctgatata agccaattcg
    33781 acttggtgtt gtgccatcca ttgacgtagt tcattgattt ttacgttatt catttggcaa
    33841 cctactttct aaattaattt tgtattcaat aattgtacca ctgatttatt aaaagaccaa
    33901 cgttctaaaa actgaaaaaa gtaaaaattt tcatgaaaac agagataaaa cggttgcaat
    33961 tcttgagatt atttgatatt ctattttaga aatgtaagac gtattttcag aaaacatata
    34021 aaatatgaat aggagaacac aaatggaaaa gcaaacaatc actatctatg atgtagcgag
    34081 agaggcaaat gtttcgatgg ctactgtttc acgtgtcgta aatggcaatc caaacgttaa
    34141 accagcaaca agaaaaaaag tcttggaagt tatcgatcgc ttggattatc gtccaaacgc
    34201 agttgcgcgt ggcttggcaa gtaagaaaac aacaacagtt ggtgtaatca tccctgatgt
    34261 cagcaatatg ttctttgctt ctttagcacg tggtatcgat gatgtggcaa caatgtacaa
    34321 atacaatatt atcttagcaa attcagatgg caacgaccaa aaagaagtca atgtattgaa
    34381 taacttactg gctaagcaag tggacggcgt gatcttcatg ggtcaccata tcacagacga
    34441 aatccgtgga gaattttcac gttcaaaaac tcctgttgta ttagctggct ctattgatcc
    34501 agatgaacaa gtaggtagcg taaacattga ttatgtatcc gcaacaaaag atgcagtaaa
    34561 caaattggca aaaagcggca ataaaaaaat cgcttttgtc agtggtgcgt tgatcgatcc
    34621 catcaatgga caaaatcgtc tgaaaggcta taaagaagca ttaagcgaaa ataacctacc
    34681 atatagcgaa ggattgatct ttgaagcaca atacaacttc aaagatggtc tatctctagt
    34741 cgatcggatc cataatagcg gtgcaacagc tgtctatgtt tcagatgatg aattagccat
    34801 cggtattttg gatggtttat tagaccgtgg cgtgaaagta ccggaagaat tcgaaatcat
    34861 cacaagtaac aattcactac tgacagaagt agctcgtcct cgtttaacaa gtatcacaca
    34921 accattatac gatttagggg cggtatctat gcgcctattg acgaaaatga tgaataaaga
    34981 agaagtcgaa gaaaaaacga ttatcttgcc ttatggaatc gaagaaaaag gatctacaaa
    35041 ataataaaag gacaaccatt cagaggttgt gtgcctgccg tttaactctg cgttacaagg
    35101 agcaatttca aaaaacagca tctcatgttt gatgaatatt gtgactcgtg attcggagtt
    35161 ggcaggagaa caccgtttat ttgggaaaag gggttatgac aaaattttgt tataatcctt
    35221 tttttgtgtc ttaaaaaaga gagaaagtgg ttgaaattac atttatcgta tataaaacaa
    35281 tccgtgtata gatgctgcat tttgttcacc accatcaaaa aaataaggga ctgtgacaat
    35341 actattgtca caatccctta ttactgtgta ttctattaaa tcaatcatct ttctcatctg
    35401 attctttagt tgcttgattc gctcttgcat aagctgaagc ggtgttccag taatctgtat
    35461 agttttcatc ggtaccgcca acgccaaatt taaaggcgct tttcttcggt ccatttttcg
    35521 cccatagcga ttcaactgtt tttcctggtg tttggatgga ttttttattg acagtaactt
    35581 ttccaggtgt ttgaccagta aatccagata cttttttctt catcacatca gatgataatt
    35641 cgaatttctc atcagtgcca aaaatgtcag gttttgcttg gtaagcttga ttgatcagat
    35701 tggcaagata attagccgaa cgtatccttg ctttgctgtc cattccagta ttgtcatcgt
    35761 gccctgccca gctactgatg gtaatagacg gagtagagac aattagccat gaatctcggt
    35821 attcattggt agaaccagtt tttccgaccc aatctgcttt tcctagattt ccgtttaagc
    35881 ttgagatggc atctttgaat ggtgtagtga tttttgcatt gatcacacta cgcatcatgt
    35941 cattcatgat cgatgccgtt gcttccgaat agatccggac agagttgctt tcgtgtttat
    36001 aaattacatt tcctgcgtta tctgtgatgg aatcgatgat atagccttct tcatagacgc
    36061 cgccgtttgc taaagcctga aatccgttgg tctgttgaag cgtcgtgaca tctactgtac
    36121 ctaaaggagc agattcaacg ccccaattgt catttgccgg gtagttcatc tttgccaagt
    36181 agtgctcgta agcatattgt ttcgatccac cggaattcaa cacatcttga tataagtgat
    36241 aagccgggat attgttggac cattctagtg actcacggac ggtttggaag gtgttcgatc
    36301 cttcgttagt agcattgacg attttctcgc cagcattttc tccttcttgc caagttgttg
    36361 cataatctga tacacgggat tctgatccga taagccccat gtcgattgca ggtccataaa
    36421 caagaactgg tttgattgat gagccagctt gacgttctgt atcgaaggca tggttatttt
    36481 gattctgact gtaattccgt ccgccaacga atccgtagat gcggccagtt ctgttatcca
    36541 tcaatacatt tccggtttct acttgactgc cagcaccatc atctaacatg tatccataat
    36601 tttcaacgcc attttgcaat gcagcataga catctttatc gattgtcgaa tggattgtat
    36661 atcctttatt ttggatcgtt tgctgtgcct tttgataata agcgtcactg accgatgaat
    36721 cagacatatc agcgttgtct ttttcagcca attgttgtgc aatgattctt gttgcttcat
    36781 tcatcacagt ataatacaag aattctctgt cagtctgttc tgcgatttgt tgtggtagaa
    36841 agtcttttgt aaggtcatag gcttttgctt cttcgtattg ttctttcgta atttggcctt
    36901 cacgatacat actgaataaa acaaaatctt ttctggcaag gccggcagaa agatcttctt
    36961 ttagcgcgcc tgtgttcgtg tatggactat acgtgatcgg gctttggggt aatccagcga
    37021 tataagcagc ttgcggcagc gtcacatctt ttgcatctac accaaagatt ccttttgcag
    37081 cttcctggac tccagcaatg ttttgtccct tattattacg accaaatggt gagacattca
    37141 aatacgtagc aatgatctca tcttttgaaa agtatttttc tacttgtgca gaaagaatga
    37201 tctcgtttgc tttacgtttg aatgttgttt catctgtaag gatctgctgt ttgactaatt
    37261 gctgtgtcaa agttgatcca ccagaagagc caataccagt agcttcagat actaatgctc
    37321 gtaaaacagc tttaggaacg taaccttggt gtttatcgaa gtattcatcc tctgtgctga
    37381 taattgcagt tttcaataaa ggtgaaatct tatcagatga aatagtcgtg cgcatcagat
    37441 ctgtctggat ttttgatatc tctgtattat ctgcataaac aatctttgac gtttctgtaa
    37501 tgtttcctag ttctgtctgc agtgcttttt tagtcggcaa ttgtgtgtct tcgacgagat
    37561 aggcaaaata gccagcgcct attccaatac ctagagctgc gaacatcaaa agaagaacag
    37621 taataaaaac agtcaatgat tgaaaaactc taaaaataat cttaggaaca aaccattttt
    37681 ctttttttct tctattctgt ttttttcgag tatttttgcg tggcaaaagg gtcacctcaa
    37741 ctaatggttt tatttcgcta attatagcaa ataatagaac aaaaaaacag acaaggctgt
    37801 ttttggcagg aatttctttt ttaaaagagt taaaacgtga gaaaatgatc tttattataa
    37861 aagagttctt aagcttttct ttagagaaaa tttcagttac tatggagaac agggcaaaga
    37921 aaaatcaatc attttctttg tatatcatct taccaatata taggaattgt tagtaacaat
    37981 tgcctttcaa ggaggaatcc atccatgtta ttaggctcac acgtcagcat gagcggaaag
    38041 aaaatgctgt taggagcagc agcagaagca gccagttatg gatcttcgac ttttatgatc
    38101 tatacaggcg cccctcaaaa cactagaaga aaagccatcg aagagatgaa tattgatgcc
    38161 ggaaaagcgt acatggcaga aaacggtcta acaaatatcg tcgttcatgc cccttatatc
    38221 attaatttgg ggaatacgat caaaccagaa atgtttcctt ttgccgtcca atttctgaga
    38281 gaagagattg ttcgtgcgga agctttaggc gctactcaaa ttacacttca cccaggtgct
    38341 catgtagggg caggaacaga agccggaatt gctagaatta ttgaaggctt gaatgaagtt
    38401 ttgacaaaag aacagacggc acaaatcgct ttggagacaa tggcaggtaa aggtacggaa
    38461 atcggtcgaa catttgagga attagcgcaa atcatcgatg gagtaaaatt aaatgaaaaa
    38521 ttatccgtta cttttgacac ctgtcatacg aatgatgccg gttataatat taaagaagac
    38581 ttttcaggtg taatggaaga gtttgataag atcatcgggt tagatcggct taaagtactc
    38641 catatcaatg attctaaaaa tccgcaaggt tcgcataaag accggcacgc caatattggt
    38701 tttggaacaa tcggattcga cacgctgaat aagatcgtcc atgatccaat gtttgacaat
    38761 atttcgaaaa tcttggaaac tccctatgtc ggcaaagata aaaagaaagc ttacgcacca
    38821 tatggaaaag aaatcgccat gttcaaagca caaatattcg atccagaagt gtttcctgag
    38881 ttattagcag aagaagtgat actctaagat tttctatctg acagcagcaa aaaaggacca
    38941 ggcacattgc cttgtccttt tttgtttaag aaacaaagga gtactttttg cagtcttttt
    39001 catcatgcat cttctccccc ctctttttaa atttagacaa acaaggtata ctgttagaaa
    39061 agtaaaaaaa cgaaaagttt cacaagcaag tggcaaaacg tgattggaag gacgaaaaga
    39121 tgctgaacac tttaaaaccc ttgatcaacc aactaaaaaa gaaacataca tactttattc
    39181 ttggcatagc cctattattt acaattattc gtttgtattt atatagtaaa gcaatctatg
    39241 gaacagacat gtcggcaacc tatgatgacc agctgctgat tcgctatgct caaaatattt
    39301 tggacggtaa atggctaggg gagtatacga ctacaacact ttccaaagga atctcctaca
    39361 gcttattttt agtacttgca cataaactgc atctttctta tgctgttttg ttcggcatgt
    39421 tcagtatttt tgccagtata ttggttgcac tcgcgtttaa accgatcgtg aagaacaagt
    39481 atcttctttt aagtatctat ttttattttt tgttttctcc gattagtttt acacatgaat
    39541 attccactcg aacatatcgt aatgccctcg tgattccggc tgttgagatc attatcgctt
    39601 gtttactagc tatttattac cgcaaatacg gttctagcag aaaattgctg ccatggtttt
    39661 tagggctttc tgtcgctttt cctttctttt ggttcattcg cgaagattct atctggcttc
    39721 ttccttttac tgtagtagct ttgggtatca gtttcgtcca gatcttgcta gggcaagact
    39781 tttctttcaa agaagtaaag aagttaaaaa aacgactctt tcttctctcg aaacgggaat
    39841 ggctgaaact ttcattgttt ttacttccta tagttggctt ttccatcgtc tatcagtgga
    39901 tcgaatataa aaatgagaca acatacggta ttgcaaccat caatgaccgt tccagtggag
    39961 aatttggacg tttgatgaaa caactgatcc gaatagatga tgaaacgaac ttgaataaag
    40021 aaaacagtga gatatgggtc tcacatgctg cactaaataa agcattagcc gcatctccta
    40081 cattccatac tgtttctacc cgtattgacg aattatatac gactcacgcg tggactcaag
    40141 gtggaaaagt aaaagaatta cccggagata tcatcttttg ggcattgcga gatgtggtaa
    40201 gcgaatctgg ttattaccaa aacaatgcaa aagaaaccaa tgatttttgg aaaaaagtca
    40261 accaagaatt agaagaagcc tatcacaagg gaaccatcaa aaagaaaaaa gagttctatc
    40321 tgatgggatc aggagatgga aagacgatga gtgatctgcc aatcgtattc gatttccttc
    40381 aatcaggata cgcttatgca tggcgatata aagatttcca tcaaggagga aattatagtt
    40441 tcggaacgca agaacaagtc aaacaggctc aagaattatt aaaccttccg ctaatggaga
    40501 attggatcga tagtaatgaa ccgagaacac ctaatttcca attaacaaaa tcagcaaaag
    40561 tcgcgaatgt catcataaaa atttaccaag ttctggctat tcccgtgctg attgcttcaa
    40621 tagcagggat attacttatg atactaggaa ccgtatttgc aaaagctgat cgtgcctatt
    40681 atagttcctc tttgattgtg gtcaccggat taatactgac acaattggtt tttcttttcg
    40741 gtgtctcgtg gttttgttct tatgcgccag aaaaaaagga ttatttttta agtgtctaca
    40801 ctggcgcagg cgtgccatta acgcaattca tcgtagtaac aagcatgcta gttttcagta
    40861 aattaaatct gaaaaaggct ttatcttcta aataaaaaga cccttggtag ctagattttt
    40921 aacctaacta tcaagggtct ttgtgtggaa aaatcagttt ctattcgatt tcttctaccg
    40981 tttcctgcaa acgctgttca tattttttgt ttcctttata taaatcacca atcgtccatg
    41041 ccaacaggag aaggacaaga acaatgatta cataagcgat gtgatcagcc aatgctagtt
    41101 gagcagaagt ggcttgttca ccaaagaaac tttcgatctg tcctggtaat cctcgtaagt
    41161 tcaagtaagt tagactaatc acagagaacc agccaagaag ttttacgatg aatgaatttt
    41221 taaatcggct acccatctcc gcttggctgt tggtcatcat cagtaaaggg atcatcgaaa
    41281 acggcaacgc aaaagccaag aacacctggg attcattcat caagttattc aaagcggtat
    41341 gttcttcgat cgttcctttt gtacttgtaa acaaaacaca aatcaataca ggaatcacag
    41401 aaagcaatcg tgtgaccagt cttcttagcc aaatcgggat acgcatatgg atgaagcctt
    41461 ccatgatgac ttgaccggtc aacgtaccag taatagtaga gttctggcca gaagcaagaa
    41521 gtgctacagc aaataaagtg gataagatac cagattttgc tacagcaatc aagataccat
    41581 tgctcatagc tgatggatca gataaggctt catacagacc aaaaaaggaa ggatcttgga
    41641 cagaaccggt tttgaagaca gcgacaccca tgatcaatag cagtgcattg actagaaaag
    41701 ccatcgacaa ttggatatta gagtcccaag tagaaaaacg tacagcttgt gctacatctt
    41761 cttcgtcttg gtgatcaatc ttccgagttt gagaaactgc tgaatgaaga tataagttat
    41821 gcggcatgac tgttgcacca ataatcccta aagctccagt cagcggagtt tcgccgccga
    41881 tgaccggtga gtctgagaat gtatgactgt tagggataaa gccagcaaat aaactgtgcc
    41941 aatctgggtt agacaagcca acttgataga caaaaacgaa taaaatgacg aaaattaagc
    42001 agacaaccag tgcttcgatc ttacgaaacc cgacttttgt cagtagtaag agaacgaaaa
    42061 catctaatac agtaataaag acggcaatca ccaatggaat atcaaaaagt aagtagaggg
    42121 cgattgctgc accaatgact tctgcgatgt ctgttgccat aatggctaat tcagtcaaga
    42181 tccataaaat gacacccaat gtcttacttg tgcgtgctcg gattgcttgg gccaaatcca
    42241 tttgggtcac gattcctaat tttgctgcca tatactgtaa aagcatagca atcagactag
    42301 acatcaaaat aactgacatc aataaatatt ggaaattttg tccaccagtg atcgaagtgg
    42361 accagttacc tggatccata taaccaactg ctactaatgc acctggtcca gagtaagcaa
    42421 gaagtgtttt aaaaaaactt ttcccctttg gtacatcaac tgttccattt acttcagcta
    42481 aagataaacc atttgtatgt tcaataagtc gatgcttatg aaattgtttg ttggattgat
    42541 tattcaagaa aaaaccacct ttcctattta tctacgatta cattctacac ctcaaaaaga
    42601 aaaaattaaa gaaaaatatt cggtatgcct aatttatttt cgtggcgcgt cttgttagat
    42661 gacgaaagaa attttgttct cctctaaaaa aaccactatt ttttgcatga aactaaagaa
    42721 tacgcctttc ttttttcaat aaactttagt aaaataagtc attgagagca attcggaaat
    42781 agaaaggaga gatttatatg ttatcaacag gtattgtcgt acttattgct attattgctt
    42841 tgctaatcgg cgcagtaggt ggctttttcc tagcgcgtaa atatatgaaa gactatttag
    42901 aaaaaaatcc tcccgttaat gaggacatgt tgcgatcaat gatgatgtca atgggacaaa
    42961 aaccatctga gaagaaaatt cgccagatga tgcagcaaat gaagaaccaa gggaaaaaat
    43021 aggttctgac ccaacgactt tttacgggct gattttttca gtccgttttt tattttgtaa
    43081 tttgttagac tattcagatt attcagcaaa aagacaagca cagacaatcc taggctcttg
    43141 ataaagacaa aggaggagta aaatgtcgat ttttagaaag ttaggctggt tttttcgtca
    43201 ggaaaagaaa cattatttaa ttggggtttt ctcgctattc gcagtggcct tggtacagtt
    43261 agttcctcca aaagtgattg ggatcatcat tgatgagatt gccgataaaa atttatcagt
    43321 aaagatcatt ttcgggtgga tcttcctatt gattatcgct gctttcttac aatatctttt
    43381 tcgttatatc tggcggacga atatttgggg aagtgcagct cggttggaga aagaacttcg
    43441 gcagcagtta ttcaaacatt ttacaaaaat ggacaatctc ttttaccaaa aatatcggac
    43501 tggggatttg atggctcatg caacgaatga tttgaatgcg atccagaatg tagcaggagc
    43561 tggtatcttg acctttgcag attcagtgat tacgggcgga acaacgatca ttgtgatgat
    43621 tttgtttatt gattggcgct taacactgat tgcgctgatt cctctgcctt tgctggccgt
    43681 cacttcacga gtgctggggt cgaaactcca tgatgctttt cgtgattcgc aagaagcttt
    43741 ttctagtatc aatgataaga cacaagaaag tatcactggg atcaaagtca ttaaaacatt
    43801 tggacaagaa aaagaagatc tagcggattt tgaagaaaag attgaagatg caattattaa
    43861 aaacaaacga gtcaattttc tagactcctt atttgatcca ttcattacct tgattatcgg
    43921 tctgtcttat gttgcaacaa ttattttagg tggaaagtat gtcttggaag gcacgatctc
    43981 gatcgggcag atggtctcat ttattagtta tatcggtatg cttgtttggc cgatgtttgc
    44041 gattggtcga ttgttcaacg tattggaaag aggaaatgct tcttatgatc gcgtcaatga
    44101 acttttgcat gaaaaaacgc atatcgtgga acaactgggt gcagtgcaga ccccggtaaa
    44161 aggcgagctg aatgtagcta tttcctcttt taattatccg aaagaagaat caccagcatt
    44221 agaacaaatc aagtttgcaa tccaagaagg agaaacatta ggtattgttg ggaaaactgg
    44281 tgcgggaaaa acaacgatca tcaaattact gatgcgtgaa tacgatgatt atcaagggaa
    44341 aatccaattt ggcggacgtt cgatcaaaga atattcattg aatgctttat taggatcaat
    44401 cggctatgtg cctcaggatc acttcttgtt ttcgatgacc attcgagata atatccgttt
    44461 ttctaatcca aaactttcag aagaagaagt agagcatgcg gcttctttag catttatcaa
    44521 caatgaaatc aaggaattac caaaaggtta tgatacctta gttggagaac gtggagtctc
    44581 tttatctggc ggacagaaac aaaggatttc gattgctcga gcattgatca aaaatcctga
    44641 attattaata ttagatgatg cactttctgc agtagatgcc aaaacagaag aagctatctt
    44701 gcataattta aaagaaataa gaaaagagaa aacaactatc attgcagctc accgtttaag
    44761 tagcgtgatg catgcaaaag aaatcattgt tgtcgatcaa gggaaaatca ttgaacgagg
    44821 aacccatcat gagctgcttg cactaggcgg ctggtatgaa caaatgtggg agaaacagca
    44881 attagaagcg aagatagaag gaggcgaggc ctgatggatg aaaattatca atcggaatgg
    44941 acaaaatcga ttccacttaa agaacagaaa aagatcatca aacgactgat gaagttcgcc
    45001 aaacctttta gaacaacgtt catcgttgct atcttatttg cgtttgctct gtctgtgatc
    45061 aatgtcctat tgccaaggat catccaaact tttatggatg atcatctggc taaacagtcg
    45121 gctacgacac aagttatttt gttttttgct ggtatgtact tatttggagt cctagtaaaa
    45181 agtatcattt ggtttttcca atggtatttg tattccatgg cttcattgaa gacctatcag
    45241 tatattaggg taaaattatt tgaaaagctg catacactag gtatgcgtta ttttgatcag
    45301 acaccggcag gatcaaccgt ttctcgagtg acgaatgaca cagaaacgct atttgaattt
    45361 tggtacgtct ttttgatggt attgactggg atctttgcag tcgtttcttc attcattgcc
    45421 atgtttcaaa tcaatggaga aattgccttg tggactttgg tgttcttgcc gattttagga
    45481 attgtcatct ggtattacca gaaatttagt tctcgtattt accgaaatat gcgggaaaaa
    45541 ttaagtcagc tgaatacaaa attgaatgaa tcgatttccg gcatgcgtat tatccagcag
    45601 tttcgccaag aagctcgttt agctaaagaa tttgaacaag tgaacaatga gtatttagac
    45661 acgcgctatg cgatgatcaa aacgaattcg attctgttaa gtccgatcat caattttcta
    45721 tatgcattgg cgattgcgtt ggcgttgact ttatttggga tcgatgcgtt gcattctccc
    45781 gtagaagttg gactgatcta tgcatttacg acttatgtcc agggattttt taatccaatg
    45841 tctcaaatga tggacttttt gagcatcttc acagatggga tggttgctgg tagtcgtatc
    45901 ttgaagatta tggatacgga agaatatgca ccacaacaaa accctggagc agatcaaatg
    45961 attacttcag gtaagattga gtttcgcaat gtaagctttt cttatgatgg agagcacaat
    46021 gtattaaaca atatctcttt tgttgctaat ccgggagaaa ccgttgcatt agtgggacac
    46081 accggaagtg ggaaaagctc gatcatcaat gtgttgatgc ggttttacga attttatgaa
    46141 ggtcagatat tgattgatga tcatgacatt cgagaatatc caatggaaga gttacgtaag
    46201 aaaatggggc tggtcttgca agatgctttc ttgttttatg gggatatcgc aggaaatatt
    46261 cggatgatga atccagctat cacagacgaa cagatacgcc atgcggctga gtttgttcaa
    46321 gcaaacaaat tcatcgatga attaccaaaa ggatatcact caaaagtgat cgaacatggt
    46381 gccagctatt ctagtggaca acgacagctg atcacttttg cgcgaacaat cgtgactgat
    46441 cctaaaattc tagtattgga tgaagcaaca gcaaatatcg atactgaaac agaaggattg
    46501 atccaagaag gattggcgaa aatgcgacaa ggacggacaa cgattgcgat tgcccatcgg
    46561 ctgtcaacga ttcgagatgc agaattgatt ttagttttag acaaaggtca tatagtagaa
    46621 cgcgggaatc atgaaacact tctagcgcaa aatggtttgt atgcagatat gtacaaactg
    46681 caaagtagtg gtatataaaa gatgttgtga cagtagcatt ttgacctaag cactagagtg
    46741 aaaataaaac atagcttccc atatttttca tctgtgagag ttagtgtcgt agttaccaga
    46801 tttaaaaaag acgacgtcag ctagtcagaa gtgcctaata gcccctaaga aagcgttgac
    46861 aaaatcgttt gctttcgata atataaagaa aacgatacaa cttggatagt gattgcctga
    46921 tgcaagctaa acctgatgag tctcccatgg tttcatggac ggctttttgg gtttttcttt
    46981 ttgttttgga tacagcccta tatatcatgg aaaggagcaa aacatgctga ttgagaaagt
    47041 actgaataac aacgtcgtaa taacaaggga tgaaagagat cgggaaatga ttgtgatggg
    47101 aaaaggattg gcattcaaaa agaaggtggg tgacccgatt cttgactcat gcatcgacaa
    47161 aaaatttcag ttgacaaatg acggactttc acaacagttc caagaattgg cacaagcgat
    47221 tcctcttgag tacttagaaa tttcttgtga aatcattgaa tatacaaaag aggctctgca
    47281 agtccattta aatgattctg tgtatatttc attgacggat cacttgtata cagctatcga
    47341 acgtcaaaaa atggggatct ttgtgccaaa tgtattgctg tatgatatca aacaactgtt
    47401 tcctaaagag tatgccattg ggaaaaaaac agtgaagaaa atctcggata aataccacat
    47461 caagttatca gaagacgaag ccggatttat cgcattgcat ttagtcaatg cacaaggcat
    47521 tcaaagccag caagacaata cctacctgtt taccacaaca atacaggata ttttaaatat
    47581 cgtccgctat tactataaga ttacatttga tgaagattca gtccactttt ttcgattcgt
    47641 cacacacctg cgtttttttc tgacacgtgt cctagatgga gcgaaatcag aagatggaga
    47701 agtttctgag gaattgttcc aagttattca aaaaaaatat aaggatacgt ttggttgcgt
    47761 tgagaaaata gcagactttt taaccggggc tttcgactac cagatgtcca cggatgaaaa
    47821 aatgtattta acgatccata ctgcgcgttt agtgcatgag aatcgttgta aataaataaa
    47881 aaaggaggac aaattaacaa gaacgtatga aataagggta gtgacattca gttggctaaa
    47941 ccttccacta attaattgga gggaatagaa atgggaaaat accaggaatt agcagaaaac
    48001 atcgtaaaga atatcggagg aaaagaaaat atccgtagct tgacacactg tgtgacgcga
    48061 cttcgtttca aactaaagga ggaaaataaa gcaaaggatg atgttttgaa aaagatggaa
    48121 gggatagtca ccatcatgaa aagcggcggg caatatcaag ttgtaatcgg gaatcatgtg
    48181 ccagatgtct atgcagaagt actgacagta gccgggattt ccgatgaagc agaagaagag
    48241 gtttcttcaa gtagtaatct gttcaatcga ttaatcgaca tattaagtgg ctgtttccag
    48301 ccttttttag gtgctttggc tgcagcggga atggtcaaag gattaaatgc tttgttcttg
    48361 tttctcaaat tttatactga aggatcgggt acgtacatca tgctgaatgg gataggggat
    48421 gcaatttttt atttcatgcc agtgatctta gggtacacag cagcgaaaaa atttcatctg
    48481 aatccgatgg tggggctcgt gattggtgca gcactttgtt atccgactgt acaaggagca
    48541 gcactacaaa gtggtttccc tgatggcagt cctgcgcctt atagcttgct gggtcttccg
    48601 gcttatgaaa catttttggg tatcccatgg gtgggcagca gttatactag tagcgtcgta
    48661 cctgttatct tcattatcgc atttgccgga caagtccaaa aaattgccaa gaaatggatt
    48721 cctgcagtcg tccagacctt tttggtttct ttttctgtgt tattaatcgc acttcctgct
    48781 ggctttttga ttatcggtcc gattgtcagt atactgacag atctattaag cacgggattc
    48841 caagcattga tggacttttc tccagctgtg tatggtgcag ttctagggtt tctttggcaa
    48901 gtactggtga ttttcggact tcattggagt attatccctc ttgcgattat gcagctagcg
    48961 caaaatgggt ggagtcagat gttagtaggc gtttatggcg cgagttttgc acagacagca
    49021 gtggtattgg cgatgtattt caagttcaag gatcaaaaaa tgaaggcgct ttgcatgcca
    49081 gctgtgattt caggaatctt cggcgtaaca gaaccggcta tttatggaat caccttaccg
    49141 aagaagaagc catttattta ctcgatgatt ggtggtgctg tttcaggaat ctatttgatg
    49201 gcatctggaa caatcagcta cataatgggc ggattaggga tttttggggt ggtaaactac
    49261 atcacggaaa cagacgccag tggtatgttt caagcgttta tcggcattgc tattgcttcg
    49321 gtaatcgggt tttcgctgac ttttttcttt tggaaggatg aagcaggtga aacagatgct
    49381 atacagaaaa aatcaggtaa agaaattgta aaaacaccga tacaaggaca aatcgctcca
    49441 ctaaatgcag cgaaggatgc agcttttgca caaggaacac taggtcgagg aatcttaatc
    49501 taccctgaaa aaggagaagt acgtgcccct tttgacggaa cagttatgac cctgttcccg
    49561 acgaaacatg caatcggaat ggtttctgaa acagggctgg aattgctgat tcatgtcgga
    49621 ttggatactg tgcagttgga agggaaatat tttgaaagct tagtgcagca aggagaaaga
    49681 gtgagacaag gcgatctgtt gctacgcttc gacaaaaaga aaatcgaaaa ggaaggatac
    49741 agtttagaaa cacctgtgat cgtcaccaat tacacggatt atttggatat tttggaaaac
    49801 caaggggaga cggtcgggcc agacgatgca cttatcaccg cactgacctg aacggttagg
    49861 tgaagaatgg actaggctta actattcttt ttattccact tctttatttt caaaattgaa
    49921 aggctctttc atgatggctg agctgatagg gcgtttatcg aatttttgac cgacagaaat
    49981 tagatggact aaattctgtt gataattttc tgcaaaaaca caactatata acacgtctag
    50041 aagataacaa attgatgtat tgatcgtaaa attaccaatt tttgaataca gtcgctcgcg
    50101 tgtcgtaagt cgcaaagcgg ctgtactcat tgcagatagc gttgaatcac cgatactggt
    50161 caaagctaaa attggaacat cattttgttt caatatttct gcggtacgaa ggatcacatt
    50221 gttttcaccc gtataagaaa tcagaatccc gcacatatcg ctcgtacaat tgtacgcttc
    50281 atagaatgat tcaccaaaag tggtgacaat tgtgacatcc attttgatgc gcttcatttt
    50341 cagcatgaaa tcttgagaaa tcaaagtatt cgagttactg gcgaagatca cgattttttt
    50401 tgcctggagc agtaaatatt tagctttttg cagttggtca tgatgaatga gagaaagtgt
    50461 atcatgaatc gtcatctgtt ctaaagaagc cattttattt gcgattgtca taataccgtc
    50521 tgtggtggaa aaaggaagat tcgcatctac gtcttgaaaa ttcccttcaa gataggctgt
    50581 ttcagctaaa aaagcattac gaaaactgac ccagccatca aaacccattt tttttgctac
    50641 acggatgagt gtagacgggt ggacataaca tcgctcagct atcgcttgga tggtcatttc
    50701 gccaagtgct ggagaatcgc ttaagagaaa ctggatcact tcattttctg aaggagaaaa
    50761 ggagatttct ttgatttttt cacgtaataa catgtagata ccttcttctt atttttttac
    50821 gtaaattata acttttttga aaatgttttg taaacggatt tatactatat aacttaaaat
    50881 aggctttttt tgacaagata tttacaaaaa tccactagaa aacagtaact tttgtaaatg
    50941 ttactggaaa gaaacggatt tctattgttt gttttgttta caaaaaagat aatgaaagtg
    51001 taaaaaagta aaggagtgag aacatggcaa aaggaatcaa aatcgctacg attggtggag
    51061 gcagcagtta cacccctgag ttgatggaag gatttatcaa aagatatgag gaattaccga
    51121 tacgagaaat ctggctcgta gatgtagaag aaggaaaaga aaagcttgag atcgttagtc
    51181 agatgtctca gcgtatgtgg gatgcttctc catatgaagt caagatctat gcaactctgg
    51241 atcgggaaga agcattgaaa gatgcggatt ttgttacgac tcagtttcgt gtcggtcaat
    51301 tagatgcacg aatcaaagac gagcggatcc ctagttatta tgggatgatt gggcaagaaa
    51361 caaatggtgc tggcgggata ttcaaagctt ttcgtaccgt tccagtcgtt ttggcaatcg
    51421 ttgaggacat gaagcgttta tgtccaaatg cttggctgat caatttcgcg aacccaagtg
    51481 ggatggtgac agaagcagtt atcagatatg gcaaatggga taaagtgatt ggattatgca
    51541 atgtgccagt gatggcgatg atgacagaac cagcaatgct tggcaagaaa aaagaagagc
    51601 tgatctacaa gtttgccggt ctaaatcatt tccattggca caaagtagca gataataaag
    51661 gaaatgaccg cacggcagaa ttgattgatc gtttatacga tgaagataat ggattaccga
    51721 aaaatatctt tgatatcccg tttttcaaag aacaattaaa acaaatgaac atgatccctt
    51781 gtggctacca ccgctattac tatcgtgaag aagaaatgct ggaacatgct ttagaagaat
    51841 atcaaacgat tggtacaaga gcgcagcaag tgaaagaaac agaagcagag ctatttgaat
    51901 tatacaaaga tccagaatta gaccataaac ctgaacaatt gcaaaaacga ggtggcgctt
    51961 attattcaga cgctgcctgt gaaacgattg cttcaatcta tggaaataaa gaaacacaga
    52021 tcgttgtatc tacaaagaat gatggagctg ttcctgacct gtcggcagat tgtgttgtag
    52081 aagtcactgc ctatgttggc gcacaaggag cgagaaatgt agcgtttggt tctcttcctg
    52141 ctgctgaaag aggctggttg caagtaatga agaatatgga attgctgacg atcgaagcag
    52201 ctgtgactgg tgattatggg acagctttac aggcgtttac gatcaatcca cttatacgat
    52261 caggaaagac agcaaaacgg atcatggatg agttgtttat tgcacaccag cagtatttac
    52321 cgcaattcaa ggaaacaatc agccgattaa aagaagaagg aatcgaagtt caagatgaac
    52381 tggcaagaga attatcaggt aatacacata taatggagga agtataatgg ggttcagaaa
    52441 agatttttta tggggcggtg ctacagcagc caatcaatgt gagggcgctt atgatgaagg
    52501 tggacgcgga ctagcaaacg tagatgttgt gccaatcgga aaagaccgtt ttcctgttat
    52561 caccggaaag aaaaaaatgt ttgaatttga tgaggatcat ttttatccag caaaaattgc
    52621 aatcgatatg tatcatcgtt ataaagaaga tattgctttg tttgcagaaa tggggttcaa
    52681 gacttatcga atgtctatcg cctggagtcg tatttttcca atgggggatg agaaagagcc
    52741 gaatgaagct ggcttgaaat tttatgagga tgtctttaaa gaatgccaaa agcacggaat
    52801 tgagccactt gtgacgatta cgcattttga ttgtccaatg catttgatca aagaatatgg
    52861 tgcgtggcgt tccagagaac tcgttggttt ttatgaaaat ctgtgtcgag tcatcttcaa
    52921 tcgctataaa ggacttgtca aatattggct gacattcaat gagatcaata tgatccttca
    52981 tgcaccattt atgggcgcag ggctgtattt tgaagaagga gaaaatgaag aacaaatcaa
    53041 atatcaagcg gcacatcatg agttggttgc aagtgccatc gcaacgaaga ttgctcatga
    53101 agtagatccg gagaacaaag taggctgtat gctggcagca ggagcatatt atccatacac
    53161 ttgcaatcct gaagatgttt gggcttctag aaaatcagat cgagaaaatt atttcttcat
    53221 tgatgtccag tcacgaggag aatatcccgc ttatgcattg aaagaaatgg agcgaaatgg
    53281 cattcagatc gaaatggaac caggggatgc cgcattgctg aaagaacata ctgttgattt
    53341 tatctctttt tcttattatt cttcacgagt ttcgactacc gacaacgaac tcttggaaca
    53401 gactgcagga aatatctttg cttctgtcaa aaatccatat ctgcaagcaa gtgaatgggg
    53461 gtggcaaatt gatccattag gcttgagaat aacgatgaat gatatttatg atcgttatca
    53521 aaaaccatta tttattgtag aaaacggctt aggtgcagtc gatacgccag atgaaaatgg
    53581 ttatgtggaa gatgattacc gaatcgatta cctggcagcg catatccagg cgatgaagga
    53641 tgcagtagaa ctagacggag tcgatctatt aggctatacg acttggggat gtatcgattt
    53701 agtatcagct ggtaccggtg agatgaagaa aaggtacgga ttcatttatg tcgatcgtga
    53761 taaccaagga aatggcacat tagcccgttc aaagaaaaaa tcattcaact ggtataaaaa
    53821 agtaattgat tcaaatggag aaaatctttc gaacaattga tcaatgattt ataaaagtct
    53881 attgtttttc agcggatttt tcgagtataa taaagctatt cgagaaacgc aagcagaaca
    53941 atagactttt tttcgcattg gctgtaaagt atgcttactt gattaagtaa aggagaatct
    54001 acatatgtat gatgtaatcg ttattggcgc aggtcctgct gggatgactg ctgctttgta
    54061 tgcttcacgt tcaaacttat ccgtattgat gatcgaacaa ggtgcccctg gcggccaaat
    54121 gaacaatacg gcagaaatcg aaaattatcc tggattcgat tcgatcatgg gccccgatct
    54181 agcttataag atgtatgaaa gtgtctcaag atttgggaca gaaaatgctt atggaatcgt
    54241 tcaagatatc aaggatcacg gcagccataa agaagtcatc tgtgaagata aaagttacga
    54301 aggaaaaaca gtcattatcg caacaggctg tatccaccgt aaattaggcg tacctggcga
    54361 ggaagaattt gctggacgag gcgtttctta ctgtgcagtc tgtgatggtg cgtttttccg
    54421 taacaaacgt ctagtagtca tcgggggtgg ggactcagca gtagaagaag ccatctattt
    54481 gacacaattt gcttctgaag tagtgatcgt ccaccgtaga gatgaattac gtgcacaaaa
    54541 gatcatccaa gaccgtgcat ttgcaaatga aaaaatttcc tttatgtggg atacagtcgt
    54601 tgacgaaatc gttggaaacg aaatggtagt gacaggtgtg aaagcacgta atgtcaaaac
    54661 cggcaatgaa tttgatttag cagcagacgg agtattcatc tatgttggac tggatccatt
    54721 aactgcacct tttagagaag caggtttgac gaacgaagct ggctggattc caacagacaa
    54781 cgaaatgaaa acgaatatgc caggcgtttt tgccatcgga gatgttcgtg aaaaagattt
    54841 gcgacaaatc actacagctg ttggtgacgg cggtattgct ggacaacaag tgtataaata
    54901 cttggagtct ttagaagaaa caacagcata agtttaaaaa gagtgaaaga acggtttcaa
    54961 ttgttctttt actctttttt ttcgcgtgaa atccactgaa tccgatttat gtgaatactt
    55021 gcaatcatgc ttgataaaag aatcctttct tgatttaagc gttttttggt cctcatactt
    55081 tcatatctaa taagtgaatg ctatacttat taagaaccat atttcagaaa gaggtgttat
    55141 tttcatgtct tgggaacaag tgtatgaaca atgggtacat gaagaaaatt tagaagaaaa
    55201 cttaaaaaaa caattaaatg atctgggtga agatcctgaa aaacttgaag atgcttttta
    55261 tgcaccgttg gaattcggta ctgcaggcat gcggggaatc ttaggacctg ggatcaatcg
    55321 aatgaacatc tatacggtaa gacaagcaac agaaggacta gcacgtttca tggatacaca
    55381 aggtgaagaa acaaaacgcc gaggtgtcgc tatcgcttat gactcacgcc atcagtcacc
    55441 agaatttgca atggaagcag ccaaaacact ggcacatcac aatatcccgt cttatgtatt
    55501 cgaaagtctg cgtccaacac cagagctttc ttttgcagtt cgtcatttga agacgtttgc
    55561 gggaatcatg gtaaccgcat ctcacaaccc agcggcttac aatggatata aagtctacgg
    55621 agaagatggc ggacaaatgc ctcctgcaga cgcagatgca ttgacgaaat atgtccgtga
    55681 agtcagcaat ccgctaaaag tcgaagtatt atctgacgaa gaagctaaac atagcgggtt
    55741 gatcacgatc atcggagaag aagtggacgc tgcttatcta gaagaaatca aagcagtgac
    55801 gattgatcgt gaattggttg aaacaatggg gaaagatttg aaacttgtgt acacgccact
    55861 tcatggaaca gggaaaatgt tgggcgaacg tgcgttgaaa caagctggat tcgaacaatt
    55921 cgtactagtt cccgaacaag ctgtggcaga tccagatttc actacggtca aatcgccaaa
    55981 tccagaagaa cattcagctt ttgaatatgc tatacgactt ggagaaaaag agggagctga
    56041 cttgctgatt gcaacagatc ctgatgcaga ccgtttaggc gcagcggttc gtttgccaga
    56101 tggttcttat gaagtattga ctgggaacca aatcggagca ttgtttgctc aatatatttt
    56161 ggaagcacat caatcaagag gcactttacc agaaaacgca gcggtattga aatcaatcgt
    56221 ttccagcgaa ttgcctacag caattgcgga acactacggt gctactatgt tcaatgtgtt
    56281 gacagggttt aaattcattg ctgaaaaaat ccaacaattt gaagaagacc attcacatac
    56341 ttttatgttt ggattcgaag aaagttatgg ttatttagtt aaaccttttg ttcgcgataa
    56401 agacgcgatc caagcattgc ttttattagc agaagtagca gcttactata aaaaacaagg
    56461 caaaacgttg tatgacggct tgcaaacgat ttttgaaacc tatggctatt tcgcagaaaa
    56521 aacgatttct gtgacaatga gcggacaaga aggttctgcg aaaatctctg ctttgatgga
    56581 aaaattccgt aaagaagcac cgacagaatt tgccggagca aaagttattc aaacagaaga
    56641 ctttaaagaa ttgacgagaa cagaccgtga aggaaacgta gaaaaactaa caacaccgcc
    56701 atccgacgta ttgaagtata cattggaaga aaatggctgg atcgctatcc gcccttcagg
    56761 aactgagccg aaaatcaagt tttatattgg tgtaaaagga agctctacag aagacgcagc
    56821 cgaaaaagta gcctcttatg aagcgattat caaaaagatg acagaagaat aaaaaacttg
    56881 agactgatgg aaaaagtggt aaactttaga agtcagtctt ttttcaagtt ggaggtgaat
    56941 ggattgccag acacgcaaaa atcaaaagta gagaaagtca gtcaattatt caaaatgttg
    57001 agtgacccca ctcgtcttaa aattctgcta tatctgaaag acggggaaca aaatgtgaca
    57061 gcaatcaccc aagcagtgga aatggagcag tctgctgtat cgcatcaact acgtttatta
    57121 agagaaaatc atgtcgtcaa gtctcatcgc gagggaaaag cgattttgta tagtcttgat
    57181 gattctcatg tactggatat tttgaaccag acactcaaac atgtggaaca tcaataacga
    57241 taggtgcaaa tagataaaca ctggagtgtg acaacgaaat ttcttcgttg tcacactcca
    57301 gtgtttttta agaaaaagac aaaaatgatt aatcgctttt ttctgttgtt tcagttccat
    57361 aataactatg atcttctagg tctaaagcat tcaaaataag cgaagccgtt tcttcaatag
    57421 ataaagatgc cacattgatc actacacatc ctagtttttc ataaagatca ttcgcaaaat
    57481 caagttcagc tctaattttt tcgatatcgg aataagcagt atcagggttt aatccgtaag
    57541 agcgcatccg ttctttacgg atattattta aaatatctgg gttattcgtt agtcccacga
    57601 tttttttagg atcgatctcc caaagctgtt tgggaatatg tgcttgtggg atcaaaggta
    57661 aattggcaac ttttaaattt ttgttagcta aaaacaagct caaaggcgtt ttggaagttc
    57721 gggatacgcc tagcaaaacg acatctgctt ctaaaaaacc acgaggatct ttcccatcat
    57781 cgtattttac agcaaattcc attgctttga tccgtttgaa atagttctca ttcaataaat
    57841 ggagggcacc aggttgccga attggcttga taccagttaa acgttcaatt tctgatatcg
    57901 gtggcgtcat cacatcaaaa ctaaataatt gatgttcttc acaaaaagaa cgtgttatct
    57961 cgatcagttc ttgattgatt aatgtatgca ggacaagtgc tttttcttct aatgcatctt
    58021 gcaatgcctg ccttaatgtt ttttcatctg tcacgaacgt tctgcggaat aggttgaact
    58081 caacggtagg atactgagcc atagtagctt gcgctagttt tgtcgcagtt tctccagcag
    58141 agtcggagat gacaaaaaaa gtaacgattt ctgtagaatc ggaagacatg gataaaacct
    58201 cacttgcatt ttttttatgt ttattatacc atactctaga atgcgtactt cgtaatcatt
    58261 ttgatttgga ggaataagtc atgaaaaaac agacattgat cgaacaatca ttagaaacat
    58321 tgaaaaataa cggttttaaa tatactaaaa aaagagaagc attgttaact tatttagtga
    58381 agcgtaaccg ctacgtatct gctaaagaag tttttgacta tatgaatcag gaattcaaag
    58441 gggtaagtta tgatacgatt taccggaatc tccatgattt tgcagagatc gatttactgg
    58501 aagaaaccga tttaaatgga gaaatgaagt ttcgttttca ttgctgtcag gggaaaatcg
    58561 gccatcacca tcattttatc tgtacagtgt gcggaacaac gaaagaaatc catatgtgtc
    58621 cgatgaatta tttcgaagaa cagctaggtg gatgcacgat cgaagggcat agattcgaga
    58681 ttttcggaag atgtgaaaat tgtcaataag gatttaaata aaataacatt tgataatttt
    58741 aaaaaaaagc aagatttcct tgacggtcaa aacagcatta gatataatat ttacaggaca
    58801 gtcttgtttt ttgagatata tttcaataac aattacggtt actttttaag ctcggaggga
    58861 gggaattaac atgtcaaaaa cagtcgttcg caaaaacgaa tcacttgacg atgctcttcg
    58921 tcgcttcaaa cgttccgtgt caaaagcggg tactttacaa gaatctcgta aacgtgaatt
    58981 ctacgaaaaa ccaagtgtaa gacgcaagaa aaaatctgaa gcagctagaa aacgcaaaaa
    59041 attctagttc tgtgatgaat tggagttggt agtgtgtcac tactaagtag attaaacgat
    59101 gacatgaaag cagcgatgaa agcgaaagat aaggaatctt tacaagtcat ccgaatgatc
    59161 aagtcatcta tacaaaacga gcagattaaa gaaggtcacg acttgacaga agaagaagaa
    59221 ctaacggtgt tgtctcgtga aatgaaacag cgacgtgatt ctttgcatga gtttgaagaa
    59281 gcaggacgtg acgatttagc tgaaaaagta aaaagtgaaa ttgtaatagt cgaaaaatat
    59341 atgccggaac aactttccga cgaagaaatt cgacaacttg ttcaagaagc aatcactcaa
    59401 actggtgctt catcaatgaa agaatttggc aaagttatgg gtgcaattat gcctaaagtc
    59461 aaaggaaaag cagatggaaa ccaagtgaat gcgattgtga aagaactatt acaatcttaa
    59521 ttttatcatt taaactgcgg acagtgatta tccgcagttt tttgtgttcg taaaaaaaga
    59581 actaaaaaac agctagaaga aagaatctta ttctttcttc tagctgtttt tttcctgttt
    59641 aatatctctt atgttaaatt gtgtttgatt tggttattct tctggtaact cacttgcttc
    59701 ttgtttatat cctgatacgc gatatacata ataaaagagc aagctgccag cgtaaccaag
    59761 caacgttggg acgacccagc caagacctaa tgcagcaaaa ggaacataat gatgataaaa
    59821 tcctagtaag cttgctacga cagtttgctg actcactaat gcaggcgaat ttgccaacat
    59881 atccaatacg gctggaacgg cagaaaagaa catcgttact tgatagacaa atggactctt
    59941 ttggaagaat tttgctgtta aggaaagtaa gattaacgca agtgacaatg gatacaaaag
    60001 catcaatact ggtaaagacc attggatgat attatccaat ccggcattcg caacgacaaa
    60061 agagacaaaa gtcgttaacc gcagccatgt catataactg actttaggga acagtttatg
    60121 gaaatcttgt gcaaaagaaa caacgagtcc catagcagta gtgaagacac ctaatgtgac
    60181 cattacccct aagaagctag caccgaagtt acctaaataa aaattagtga tctgagaaag
    60241 tgcagttccg ccattttctg ataaagacat cttattcaaa ctcatcgcac cgagaaggac
    60301 cagaccaaaa taaatcatga cttctaacgc aacgctcagg ctaccagctt ttgctgttaa
    60361 ttctgtgact tttttatctt taaaaccaag atcacgaact gcatggacaa aagtgacact
    60421 taaagcaagt aaagctactg catcaaccgt gttgtatcct tctaaaacgc catttgtcaa
    60481 agcgttcgtc tgataagaat ctgcagcaga ttggtttaga ttccccatgg gttggaataa
    60541 agaaacgaca aaaatgatag ctaataatgc aagaaacaag gagtttaaat actttccgac
    60601 atatttgacc aaacgtgttg gatgtacagt aaggaaataa gcaagtaaaa agaaaagagc
    60661 agagaataaa aacataccta atgaatcata tttttcaggc aaaaagggtt tagcagccat
    60721 ttcaaaagca gttgctgctg tccgcggtgt accaaagaat ggtccgatcg ttaaatgaac
    60781 aaggatcaag aacaaggcag cataccattt tccgacaggt ttagctaagt catacagacc
    60841 atcacttttt gtcactacta cagctaagat agctaataat gggaaaagag cgcctgagat
    60901 ggcaaatcct acaccagcgc tgatccagtt tgatccagcc atttgaccta agtgtacagg
    60961 aaagatcaga ttacctgacc cgaagaacat cccgaagata agtgaactga ccatcaataa
    61021 ttgcttccat gaataagttt ttgtttcttc cataaatttt tcctccaata attatcattt
    61081 tgagtaacaa aataaacgtt gcagaaacta gaaataccac taatatcaga ttccatgaaa
    61141 accaccacct taaaatataa aaaaagccct tttccacttg ggaaaagggc tctagattct
    61201 agaacggtac cacctttttt cgcttaaaaa agcttcttca ttcacggacg cttttcgata
    61261 aagttatccg ctagcctgat aatggaggca gccacaatga tctacgtttt cgacattgac
    61321 tcggaaatga tgttcgtcta ttcgatttat tgtcatttct cacttacatg actcactggg
    61381 aataaatcac tgaatagcct actgttttcc tcgacgcttt gctgtatatt tatttgttga
    61441 cgttataata cacctgtttt ttttgaaatg caagaatatt tcaaatattt tgttttcttt
    61501 ttttgaaaca agaaaaatac ccagagaata cttgctaagt tagacggaat acttgtcctc
    61561 tatcttatgc atagcttaca tgatcagctt tttttattca aacgagacct ttagagaatg
    61621 gaagcggcta gttttttaac gtttcttggt acttatggtt aagcaactct tttagaacct
    61681 tttcttattt gctatatagt ttcttctgag tatggtaaaa taggaaagaa tgaatcgaaa
    61741 aaaaggggat gtctattttt gacggaagag tctagttctt tagagattag attgacggct
    61801 gctgatgatg tcagcatgct tttaggtgca cacgacaagc atataaaact gattgaggaa
    61861 acaacggaaa caaccatcca tacacgtgga gaaatcattc agatcattgg tgagcaatcc
    61921 cagatttcct tggctgcctc agtcattcgg acattacagg aattgatcaa acgaggaatc
    61981 cacgtaagta cgcccgatgt tgtgacggca ttgaaaatgg ggcaaaaagg tacattagat
    62041 tattttgttg agatgtatga ggaagagatc gtcaaagacc gtaatggaaa acctatacgg
    62101 gtaaaaaaca gcggccaaaa gaaatatgtc gaatcggtag ccaaacatga tatcgtattt
    62161 ggcgtaggac cagccggtac aggaaaaact tttcttgcag tagtattagc aatcgctgcc
    62221 ttgaaaaaag gtgaagtgca aaaaatcatt ttgacgcggc ctgctgtgga agcaggtgaa
    62281 aatctaggct ttcttcccgg ggatctaaaa gaaaaagtgg atccttatct gcggcctgtt
    62341 tatgatgctt tataccaaat ttttggattg gatcatacga accgattgat ggaacgaggc
    62401 gtaatcgaaa tcgcaccttt agcttacatg cgggggcgaa cattggatga tgcattcgtt
    62461 atcttggacg aagcacaaaa cacgacagtc gctcagatga aaatgttctt gacccgtctc
    62521 ggttatcaat caaaaatgat tgtcaatggg gatacaagtc agatagactt gcctcgaggg
    62581 acgacaagtg gtcttgttca tgcagaacga acattaaagc aaatcaagaa aatcgatttt
    62641 gttaattttg aagcaagtga cgttgttcgg caccctgtag ttgccgaaat catcaaagct
    62701 tatgaaaagg cagatctcca tcaagaataa gctttgctta ggaggataag cacatgttga
    62761 ataaaacact gcagaaacta caagaaaaac ttggaaaggc agcattgcct ttcctgctgt
    62821 tcttgttttt tatcattatc tgcggcgtta catttagtag tgtcaaacaa aaggccaatg
    62881 atttcagaga aggccaagta gcagaagaaa gtatccgtgc gaacaaaacg atcgagaaca
    62941 cggaagaaac cgaacaaaaa agaaaactgg cagcagaagc agtcactcca gaatacactt
    63001 accaaaaaga tcttgcagat gaccagaaca atcggatcaa gcaactattt gaattgatcg
    63061 ataagacgaa tgatgccatc aacaaatctt atgacgaaaa gattgataag gcaaaggaag
    63121 atgaaaatat cccaaagcct actacagaag agcggatcgc cgcgctgaag aaaaattttg
    63181 aaaatgtgaa tgcagaaaat gttgtgtttt atcaaaaact accgaacaat ttttattcga
    63241 cgatatttga aatgactgaa actgagattc atactgtgag ggatgagagt ctgaagctca
    63301 ttgacgaaca gatgagcaaa caagttcgtg aaagtgaatt ggaagctttt aaacaagaag
    63361 cagaagagca aatccaatat ttgaatgtca caccagcaca acaacaaatg attcgctact
    63421 tagtggatca aggtatcgta gtgaatgacg tattgaatga gaaaaagaca gaagaattga
    63481 aacaatccgc acgagaagct gtacaaccag tgatgatctt ccaaggagaa atcattgttc
    63541 gtgaaggtaa tcaaatcgat gcttcagcta tgaaaaaatt ggaattacta ggactgacta
    63601 atcaaacaac ttcgattttt ccgctaattg ccatggtttt agctgttttg cttcaaatcg
    63661 gcgtactgat ttataattca cttcaattcc aaaatgcagg agaaagaaca aaatacgtgc
    63721 ttttctatgt gacagctatg tcagtcagca tagtgctgat gaaatttttc caattattcc
    63781 aaacggaaca agctacttat atcccgctgt tttatcccgc tgcttttgca ccacttgttt
    63841 taagcttttt cttgaatcga agagcaggga tcatggctgg tattttccaa gcagtatccg
    63901 cacttttcat tttttatgat tcgattggca cgaactttct aacgattatc ttgatgtctt
    63961 atgttttctc tgggctcatg gcaacgattg tcagacgcaa acggatctca gaacaaggat
    64021 tatctgctgc catgtgggtc attatttttc ctgtgatgat ggatgtcatt ttgatcgtct
    64081 atcaaggaat gagctttggt gatgcaacga cttggttaat gctcgtctgc gggttcactg
    64141 gtgcactctt ctcattcttg ctgacgatcg gtctacaccc atatattgaa ttgatggtca
    64201 atgatgacag tgtaattgta ttaaatgaac tcagcaatcc aaatcatcct ttattgaaac
    64261 agttgctgga agaagcgcca ggtacttatc atcatagtat gatggtagct aatctaagtg
    64321 ctaacgctgt agcagaaatc ggcggacgtt ctttgctaac tcgagtagct tgctattatc
    64381 atgatatcgg caagatcaaa catgctagct ttttcgttga aaacttgcct tcaggagcgg
    64441 aaaacccgca caatttctta ttgccagaag acagtaaaca tattatcttt ggacatgtga
    64501 cagatggagc caaaatattg gaagattata atatgccgca aatggtgatc gatatttgca
    64561 aacagcatca tggaaccacg ctaatgcgtt atttctacat caaagcgaaa gaacgcaatc
    64621 cggaagtaac agaagagcaa ttccgttatc ctggaccaaa accgcaaagt cgtgaagcag
    64681 ggatcgtcaa tattgctgac agttgtgagg ctgcagtacg ggcaatggat catccgacga
    64741 gcgaaaaaat cgaaagtttt gtccataatc ttatcgaaga gcgtatttcc gatggtcaat
    64801 tagacgatag tggactgacc ttgaaagaaa tccgaaaagt ggaaaaatca ttaatcagcg
    64861 gtttaagtag caccttccat tctcggatca aatatcctaa aatgaaaaca gaagttgaga
    64921 agataaagga agaacaagaa gaatagaaag aagcgatgta taatggatat tacctttatg
    64981 gatgaaacaa accaagtctc agaagagaaa atgaaagaaa tcgatgactt gctgcaattt
    65041 gcagcatctt atttaaagct gccagaggaa acagaaatgt ctgttacttt catggacaat
    65101 gcggcaatcc aagtaatcaa tcgtgattac cgtggaaaag atgcaccgac agacgtcatc
    65161 agttttgctc tggaagaaga aggcgaagac gaaatcccag tgatttttga agatggagag
    65221 aatccattac ctcgtgtact aggagacatc atgatctcta ttgaacgtgc tgctgaacaa
    65281 gcagaagctt acggccattc atatgatcgg gaacttggct ttttagcggt acatggattc
    65341 ttgcatatca acgggtatga ccatatgaca ccagaagatg agaaagaaat gtttggttta
    65401 cagaaagaga tcttggatgc ctatggactc aaaagataaa aacgtagaga aaaacaaaca
    65461 tttcctgatg tctgtggaat ttgctgtcca aggagtcaaa accgtttttg aggaagaacg
    65521 caatatgagg aaacatgttg cgttcggtat ccttgcttta tttatgggcg tgattttcca
    65581 gcttgatcgg atagagtggt tatggctgct tttagctgtt tttctcgttt ggatagtaga
    65641 gatcatcaat actgtttttg aaaatgtagt ggacatgttt acggattttc attttcatcc
    65701 gattgggaaa aaaatcaaag atatggcagc tggagcagtt ttagtgaccg cttgttttgc
    65761 tgtcatcatt gggttgattt tatttgtccc taaaatatat catttgttgt ttaattaaaa
    65821 aggagaacgt atatgccaga acataaatca gggtttgtcg caatcgtcgg ccgcccgaat
    65881 gtaggaaaat ccacattatt gaaccgaatc gtgggtcaaa aaattgccat tatgagtgac
    65941 aaggcacaga caactagaaa taaaatccaa ggagtatata ctacaccaga agctcagctg
    66001 atttttatcg atacaccagg aatccataaa ccaaagcatc gtctaggaga ttttatggta
    66061 gaagcagctt acagtgcgtt gaaagaagtg gatgcagtct tatttatgat cagtgctgat
    66121 caaaaacgtg gccggggaga cgattttatt attgaacgtc taaaaaacgt gcattctcct
    66181 gtttatctga ttatcaataa aatcgataag atccatccag acgagttgct gggaatcatc
    66241 gaagattatt cgacgcagat gccttttgca caagtgattc cgatctctgc tacagaagga
    66301 aacaatgtcg aacgcttaat ggatgtatta gtcagtgaaa tgccagaggg cccacaatat
    66361 tttccagatg atcaagtgac tgatcatcct gaatacttca tcgtttctga attgatccgt
    66421 gagaaagtct tgtttttaac acgagacgaa gtgcctcatt ctgtcgcagt cgttgttgat
    66481 tcgatgaagc gaaacgagaa tgataaaatc catatccagg ccacgatcat cgtcgaaaga
    66541 gatagccaaa aaggaatcat tatcggcaaa ggcggaaaaa tgctgaagca agtaggaacg
    66601 aaagcaagaa aggatatcga aaatctttta ggagacaaag tatttctaga actttgggtc
    66661 aaagtccaaa aagactggcg agacaaaaaa gtctatctgc aagacttcgg ctaccgaaaa
    66721 gacgagtact gatattctgg acttcttata ggttaggtat agaaataaac tggataaaaa
    66781 tccgtacgtt gagaaaaaca gcggaagatc gaaacataaa taatctggat cggcagaatc
    66841 tgcttttcca gatttatttt ttaggaggga gaatatgagc cagcttgcag aatcgaaagg
    66901 cttgatttta tctagccgta attacaaaga aaaagataaa ttagtcaaaa tctttacgga
    66961 atcttctggc aaaatgatgt tttatatcaa aggaatccat cggcaaaatc agccattagc
    67021 tccggctttg ctccccttta ccgaagcagt ctatatcggt aattttcgaa gtgaaggttt
    67081 gtcgttttta agcagtgtca aagacgtcca tgcgtttcgg aacatccaag aagatatctt
    67141 tttgagcggt tatggtacgt atttgctaaa tctagtagat gcagcaatcg aagatcatca
    67201 atatgacccg catttattcc aatttacgca acaagctctg cagcggatgg accaaggaga
    67261 tgatgcagaa atcatcacga atatctttga agtgcaaata ctgcaacgct tcggaatcgc
    67321 gccaatttgg acgcactgtg tcgtatgcgg ggaaacaaaa ggaaaatttg actattcttc
    67381 caaatatggc ggtgtgatat gcgagaaaca ctggccaatg gacgaacacc gttatcatgc
    67441 agatcctcgc gctgtttatt tcgttcgaat gttttcggct atttcttatg ataagatcag
    67501 tggaatccac ttgaaagaag aaacgaagca ggcaatccgt gacctcattg accagttgta
    67561 tgatgaatat gtcggtattc atctaaaaag caaaaagttc attgatcaaa tgaagtcttg
    67621 ggcacacgta ttaaagacgc cagagaaaaa tatgcctgaa aaagagatac ctgaaaaaga
    67681 agaataaatg aaagatacaa caaaaaaatc aaaactattt gacaaataca gggacttccg
    67741 tctattatta accgtagatt aaatagatcg ttcactgaga ggatctatta tacaaaccag
    67801 aaaactgcaa aatgaatacg tacattgacc aagatcagta aaaaacacgt ggtttagcga
    67861 gttcgggaga gtggaagccg gacccaaacg atttttgaag aggcacttgc gagtacgata
    67921 aatgaagctg ccgaatcttt attcggaagt agggtggaac cgcgaaatta ttcgtcccta
    67981 tgtctgtaat cagacatagg gactttttgt cgttttcttt cctgtattct tattgacagc
    68041 aacgcttaaa aattgaaagg ggcaaaacag atgagtaaaa aattgaccgt ccaagatatg
    68101 attttaacat tacaaaaatt ttggtcatca aacggctgca tgctgatgca agcttatgat
    68161 acagaaaaag gagcaggtac gatgagccca tatacattct tacgggcaat cggaccggaa
    68221 ccatggaatg ctgcttatgt agagccatcc cgccgaccag ctgacgggcg ttatggagaa
    68281 aacccaaacc gtttgtatca gcatcatcaa tttcaagtcg taatgaaacc ttcacctgaa
    68341 aatatccaag agctttattt agaaagcttg cgtcttttag gaattgatcc attagaacac
    68401 gatattcgct ttgttgaaga caactgggag aacccatcga tgggttgtgc cggtgtgggc
    68461 tgggaagtat ggcttgatgg gatggagatc acacaattca catacttcca gcaagtcgga
    68521 ggactacctt gtaaaccagt tacttctgag atcacttatg gactagaacg tcttgcttct
    68581 tatatccaag aagttgaaag tgtttatgat ctggaatggg ctgatggcgt gaaatatggt
    68641 gagatcttca aacaaccaga atatgaacat tctaaatatt cttttgaagt aagcgaccaa
    68701 gacctgctac taagcaattt tgatcgtttt gaaaaagaag ctaaacgctg tatcgatgaa
    68761 aatcttgttc atccagcgta tgattatatt ttgaaatgta gccatacatt caatttatta
    68821 gatgctcgcg gtgcagtatc cgtaacagag cgtgcaggtt atctttcccg tatccgcaac
    68881 atggctcgtg ctgtagccaa aatctttgtg gcagaacgcg aaaaattagg attcccttta
    68941 ttgaataaaa acacagcaaa tccagaaaac gtgaaggagg ctgaataaga tggcaaaaga
    69001 cttattactt gaaatcgggc tggaagaaat acctgctcat gtagtcacac caagcatgaa
    69061 acaattagaa gaaaaaacag caaaattttt agatgaaaac caattgactt atgatacgat
    69121 cgaaacgttt tctacaccgc gccgcttagc aatcaaagta acaaacattc cagaacgtca
    69181 agcagacagc gaagaagaag tcaaaggacc agccaaaaaa attgctttgg acgcagatgg
    69241 aaactggacg aaagcagctg aaggatttgt ccgcggacaa ggtctgacga cagatgctat
    69301 cacttttaga gaattaaaag gcgtggaata tgtctatgta acgaaataca ctcacggcaa
    69361 agaagcaaaa gaagtattga caggtttagc agatgtcatt aaaagcctca ctttcccagt
    69421 aacgatgcac tgggcagaat acgattttga atatattcgc cctattcact ggatcgtggc
    69481 attgttagat gacgaaataa tcccgtttga gatcttggat gtcaaaacag acagagcttc
    69541 aagaggtcat cgtttcttag gcgaagatgt gacatttacg catgcaaaac aatacgaaga
    69601 aaaattgaat gaacaatttg ttattgcttc accgcaaaaa agaaaagaaa tgatcatcac
    69661 acaagcaaac gaattagcag aaaaaaatca ctggatcatt gatctagatg aagatttact
    69721 agaagaagtc aacaatttag tcgaatatcc aactgtcttt tctggcgact tcgaagaaaa
    69781 atatctatct gttcctgaag aagtactagt cacttctatg aaagaacatc aacgttattt
    69841 cgaagtacgg aacgatcaag gtatgttgct tcctcatttt atttctgctc gtaatggcga
    69901 tcgggtaaaa ttggaaaatg tggtacgagg caatcaaaaa gtattgactg ctcgtttaga
    69961 agatgcggaa ttcttttata atgaagacaa gaaagcttca atcgaccaat acgttgaaaa
    70021 attaaaaact gttactttcc atgaaaaaat cggtagcatc tatgagaaaa tgcaacgggt
    70081 acaagtcatc gctcgcatca taggcgaaca agtaggacta tctgaggaag aattgatgga
    70141 tctgcaacgc gctgctgaaa tctataaatt tgatctagta accaacatgg taggagaatt
    70201 ccctgaacta caagggatca tgggcgaaaa atatgctctg cttaaaggcg agaaaccagc
    70261 cgtagcaaca gcgatccgtg aacattacat gcctatatcc agtgaaggag atttacctga
    70321 gactacagta ggttctgttt tagcgattgc tgataaatta gatagtatct tttctttctt
    70381 tgcagttggg atgatcccaa gtggctcaaa cgatccatat gctttgcgcc gtcaagcata
    70441 tggtgtcgtt cgaatcatcg aatcgaaaaa atgggccttt cctttgtctg ttctgcaaga
    70501 aacgattagt gaagttatca gtaaagatac ggatcgtttt ggcattggct tgtcagcggg
    70561 acaacaacag gtgatcgact ttatcaaagg ccgtctgcgc caattattga caacgaagaa
    70621 tatccgtcat gatgtcatcg aagctgtttt gaatgcagaa caaaaagatc tgacaaaagt
    70681 atttgcagca gcacaattgt tcaagcagca tcttgcagat gaagacttca aaccatcgat
    70741 ggaagctttg acacgtgtcg tgaacttagc gaaaaaagcg gagcttgaaa aacaatcaga
    70801 agtagaccct gaactgttcg aaaacgaagc ggagaaagaa ctgcacaaag cagtagaggc
    70861 aatgaaaaca agctttgctg accaaacgat caacgaaaat tatgatcagc ttgtaagcct
    70921 tcgcccactg atcgaaaatt attttgatca gacgatggtc atggcagaag aaccagcagt
    70981 acgtaagaac cgattaacgc aattaagtca aattgctgcc atggctttgt cactagcaag
    71041 tttagatcaa atcatcacaa aataatttct aatatgtaaa taaaccagag aacaggcaca
    71101 aaataggcta tttctctggt ttatttgtta taaatataaa taactattat taattcaatg
    71161 ttaatttttt tgttatttta ttttttgtgt aacttttaaa aacacctatg a
//
LOCUS       DAYYME010000002        60427 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000002 DAYYME010000000
VERSION     DAYYME010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.002"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1421)
                     /locus_tag="ACHBFW_000070"
     CDS             complement(<1..1421)
                     /locus_tag="ACHBFW_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFM9316420.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAG"
     gene            1673..2854
                     /locus_tag="ACHBFW_000071"
     CDS             1673..2854
                     /locus_tag="ACHBFW_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9316421.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(2914..4542)
                     /gene="treC"
                     /locus_tag="ACHBFW_000072"
     CDS             complement(2914..4542)
                     /gene="treC"
                     /locus_tag="ACHBFW_000072"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFM9316422.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(4993..7704)
                     /locus_tag="ACHBFW_000073"
     CDS             complement(4993..7704)
                     /locus_tag="ACHBFW_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM9316423.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7714..8415)
                     /locus_tag="ACHBFW_000074"
     CDS             complement(7714..8415)
                     /locus_tag="ACHBFW_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9316424.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8601..9200
                     /locus_tag="ACHBFW_000075"
     CDS             8601..9200
                     /locus_tag="ACHBFW_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM9316425.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9281..10294
                     /locus_tag="ACHBFW_000076"
     CDS             9281..10294
                     /locus_tag="ACHBFW_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFM9316426.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10402..11319
                     /gene="rbsK"
                     /locus_tag="ACHBFW_000077"
     CDS             10402..11319
                     /gene="rbsK"
                     /locus_tag="ACHBFW_000077"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFM9316427.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11347..11742
                     /gene="rbsD"
                     /locus_tag="ACHBFW_000078"
     CDS             11347..11742
                     /gene="rbsD"
                     /locus_tag="ACHBFW_000078"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFM9316428.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11742..13115
                     /locus_tag="ACHBFW_000079"
     CDS             11742..13115
                     /locus_tag="ACHBFW_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9316429.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13472..15616
                     /locus_tag="ACHBFW_000080"
     CDS             13472..15616
                     /locus_tag="ACHBFW_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM9316430.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLKK"
     gene            complement(15668..16708)
                     /locus_tag="ACHBFW_000081"
     CDS             complement(15668..16708)
                     /locus_tag="ACHBFW_000081"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM9316431.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(16985..18787)
                     /locus_tag="ACHBFW_000082"
     CDS             complement(16985..18787)
                     /locus_tag="ACHBFW_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9316432.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18801..19553)
                     /locus_tag="ACHBFW_000083"
     CDS             complement(18801..19553)
                     /locus_tag="ACHBFW_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9316433.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19562..19924)
                     /locus_tag="ACHBFW_000084"
     CDS             complement(19562..19924)
                     /locus_tag="ACHBFW_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM9316434.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20100..20972)
                     /locus_tag="ACHBFW_000085"
     CDS             complement(20100..20972)
                     /locus_tag="ACHBFW_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM9316435.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21023..21511)
                     /locus_tag="ACHBFW_000086"
     CDS             complement(21023..21511)
                     /locus_tag="ACHBFW_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM9316436.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(21649..22440)
                     /locus_tag="ACHBFW_000087"
     CDS             complement(21649..22440)
                     /locus_tag="ACHBFW_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFM9316437.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22495..23103)
                     /locus_tag="ACHBFW_000088"
     CDS             complement(22495..23103)
                     /locus_tag="ACHBFW_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFM9316438.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23096..23413)
                     /locus_tag="ACHBFW_000089"
     CDS             complement(23096..23413)
                     /locus_tag="ACHBFW_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9316439.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23569..24297)
                     /locus_tag="ACHBFW_000090"
     CDS             complement(23569..24297)
                     /locus_tag="ACHBFW_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFM9316440.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24456..25337
                     /locus_tag="ACHBFW_000091"
     CDS             24456..25337
                     /locus_tag="ACHBFW_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9316441.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25574..26617)
                     /locus_tag="ACHBFW_000092"
     CDS             complement(25574..26617)
                     /locus_tag="ACHBFW_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9316442.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26620..27288)
                     /locus_tag="ACHBFW_000093"
     CDS             complement(26620..27288)
                     /locus_tag="ACHBFW_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9316443.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27430..29766
                     /locus_tag="ACHBFW_000094"
     CDS             27430..29766
                     /locus_tag="ACHBFW_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFM9316444.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29881..30192)
                     /locus_tag="ACHBFW_000095"
     CDS             complement(29881..30192)
                     /locus_tag="ACHBFW_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316445.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30373..31848
                     /locus_tag="ACHBFW_000096"
     CDS             30373..31848
                     /locus_tag="ACHBFW_000096"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFM9316446.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31895..32479
                     /locus_tag="ACHBFW_000097"
     CDS             31895..32479
                     /locus_tag="ACHBFW_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFM9316447.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            32481..32864
                     /locus_tag="ACHBFW_000098"
     CDS             32481..32864
                     /locus_tag="ACHBFW_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFM9316448.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(33181..33465)
                     /locus_tag="ACHBFW_000099"
     CDS             complement(33181..33465)
                     /locus_tag="ACHBFW_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFM9316449.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33817..34527)
                     /locus_tag="ACHBFW_000100"
     CDS             complement(33817..34527)
                     /locus_tag="ACHBFW_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFM9316450.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34524..35996)
                     /locus_tag="ACHBFW_000101"
     CDS             complement(34524..35996)
                     /locus_tag="ACHBFW_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM9316451.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36134..36607
                     /locus_tag="ACHBFW_000102"
     CDS             36134..36607
                     /locus_tag="ACHBFW_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9316452.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36760..37311)
                     /locus_tag="ACHBFW_000103"
     CDS             complement(36760..37311)
                     /locus_tag="ACHBFW_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9316453.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37416..37946
                     /locus_tag="ACHBFW_000104"
     CDS             37416..37946
                     /locus_tag="ACHBFW_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFM9316454.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38429..38872)
                     /locus_tag="ACHBFW_000105"
     CDS             complement(38429..38872)
                     /locus_tag="ACHBFW_000105"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9316455.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38888..40048)
                     /locus_tag="ACHBFW_000106"
     CDS             complement(38888..40048)
                     /locus_tag="ACHBFW_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9316456.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40555..42255
                     /locus_tag="ACHBFW_000107"
     CDS             40555..42255
                     /locus_tag="ACHBFW_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316457.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42357..43190)
                     /locus_tag="ACHBFW_000108"
     CDS             complement(42357..43190)
                     /locus_tag="ACHBFW_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFM9316458.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43494..45659)
                     /locus_tag="ACHBFW_000109"
     CDS             complement(43494..45659)
                     /locus_tag="ACHBFW_000109"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFM9316459.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45919..48213
                     /locus_tag="ACHBFW_000110"
     CDS             45919..48213
                     /locus_tag="ACHBFW_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFM9316460.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48206..48871
                     /gene="pgmB"
                     /locus_tag="ACHBFW_000111"
     CDS             48206..48871
                     /gene="pgmB"
                     /locus_tag="ACHBFW_000111"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM9316461.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            48864..49871
                     /locus_tag="ACHBFW_000112"
     CDS             48864..49871
                     /locus_tag="ACHBFW_000112"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM9316462.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49893..50912
                     /locus_tag="ACHBFW_000113"
     CDS             49893..50912
                     /locus_tag="ACHBFW_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9316463.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51193..52560)
                     /gene="nhaC"
                     /locus_tag="ACHBFW_000114"
     CDS             complement(51193..52560)
                     /gene="nhaC"
                     /locus_tag="ACHBFW_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM9316464.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52669..54090)
                     /gene="tyrP"
                     /locus_tag="ACHBFW_000115"
     CDS             complement(52669..54090)
                     /gene="tyrP"
                     /locus_tag="ACHBFW_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFM9316465.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54333..56210)
                     /gene="tdc"
                     /locus_tag="ACHBFW_000116"
     CDS             complement(54333..56210)
                     /gene="tdc"
                     /locus_tag="ACHBFW_000116"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM9316466.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56501..57757)
                     /gene="tyrS"
                     /locus_tag="ACHBFW_000117"
     CDS             complement(56501..57757)
                     /gene="tyrS"
                     /locus_tag="ACHBFW_000117"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFM9316467.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57869..58074)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58337..59638
                     /locus_tag="ACHBFW_000118"
     CDS             58337..59638
                     /locus_tag="ACHBFW_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM9316468.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59707..60306)
                     /locus_tag="ACHBFW_000119"
     CDS             complement(59707..60306)
                     /locus_tag="ACHBFW_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9316469.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        18243 a        12359 c        10828 g        18997 t
ORIGIN      
        1 tttcctgcat ataaatgaaa atgttcggag tcagccggtg caatcgtgtg atcactgaat
       61 tgaatatatc taaaagcgcc actttcctta tcttctgctt cgaatagata ccttacgcca
      121 cggtttcctg cctcgtaggt caagatttct ttccctacat aacgataatt tgaacgtttc
      181 gttgttccat tcttaaacgt aaatgccatc tctttctcac tgatcttgat tgaagcaata
      241 tctgtttgat agcccttcgt ataatattct ttatattctt gtgctgtctt atcctgattc
      301 aatttttctt tatattcaaa aacaggatca aaagtcccat cttctacata cgtatctact
      361 gtttgccaag ttcctgccca atctgaaaga gagcgatctt gtacatcctc gtcttcaaaa
      421 taaccgttgt agacgctctt ttcttttttg ggggtcaaag tatcttctag cgggttttct
      481 gtctctgtcg tttttttcaa cgcttcgagg tttgcttcca tgatagaaag atagttttct
      541 ccattttcta tttgttcatt cgtcaatcct tccaaaggat tcaatacttc taaagataca
      601 cctgcttcgt ttgctaacgt tctggcaata ctatctttgg cgttttcttc aaaataaata
      661 tagttgattc catgttcttt aacaaagtgt ttcagttcag caattctcgc cgcagacggt
      721 tcttcactag aagaaagacc agcaatcgat acttgattca atccatattc taacgctaga
      781 tagttgaatg cagcatgttg cgtgacaaaa gatctttgtt ttgcttgctt aaaggtttct
      841 tgataaagct gatggagtgc ttgaagtttc tcggtatagg ctgctgcatt ttttgtccat
      901 acttcctgtt tttctgggta agcttcaatc agctgatcac gtatgtttgc tacctgcttg
      961 attgctaaac ttggagctag ccatacatgt gggtctagct catgtgtatg gccttcttcc
     1021 ccgtgttcat ggtcatgatc atgtccttct tcacttcccg gaagtaaaag cataccttct
     1081 gtcgctttta tcgtatggac attgccttct tgtaatgttt tttggattgc ggggacccat
     1141 gtttccatgt tttcatcatt ataaacaaag atatctgtat cttggatctt tgccatgtct
     1201 ttagcagaag gttcataatc gtgggaatca gtccctgctg gaatcaataa ctctacttct
     1261 ccttcatccc cgacgattgc ttttgtaaaa tcatacatgg gataaaaggt cgtcatcacg
     1321 ctcaattttt ctttactgtc ctcggctttc ccacttgccc cgcaagcagt taacatacct
     1381 gctaaaacca gcatggccaa tatacctatc aatcgtttca tttatgacac tcctcttctc
     1441 atcttctttc atttgaatgt tccgattcgc ttttcgcaaa ccgtaatagt tacgttttag
     1501 caacaggatt taatttatca tatggttaaa agaactgtca agcgttttct tattttaatt
     1561 gatgagaaag tttatatgaa tgattgacga tatttttgca atcaacctct atcattttcc
     1621 ttttataccc gcttttgtct ataatgaagt tatagataaa ggaggaaaaa gagtgaaaaa
     1681 agaaacagat acaacgaatc gtttgatccg ctttttagga ggaaaaacct cttactatgt
     1741 tttaggaata gttatcttac ttgcgctgat catttatttt ttccatcaga tctcgtttat
     1801 attcaagcct gtcggaatca ttatcgctac tatcttgccc ccattattgt ttgcactgat
     1861 cttgtattat cttttggaac cagtcatcaa acgtttgagc aagcaccttt cgaggaactt
     1921 gtcagtcatt atcgtttatg tgattatttt atttttattg gggcttggaa gcatatggct
     1981 tattcctttt cttgaagaac aggcaaagac attgatcagt agtttgccag atttgtttca
     2041 agattttcaa gagagtctcc gacaattttt ggagaaaaca ccttttgccg attcatttaa
     2101 tcaatttttc gcttctattg acgacgtgac gtctgatata gctggattta ttggaaatta
     2161 ttgggaaagt ggcgcacaac ggatcagcaa cattttttca actgttacag ctatttttat
     2221 tactttattt actggtccaa ttgtcgcttt tttccttttg agagatcctc aaaaatttta
     2281 tcagtcagtt ttagcgattg tcccaccagc atttcgtaca gattttaaaa atttgacaaa
     2341 gattgctaat caacaattgg gttcatttct aaaggggcaa atcattgctt catttatcct
     2401 tggagcagtc tactgggttt gtttcttact gatcggatta gaatttgcta gtgttttagc
     2461 tatttctgct gggttgttat gtatcattcc atatattggg ccgtttatcg ccttctttcc
     2521 aggtttgatc attgcattcc aagactctac ttttatggct gtcaaatttg tcattgtctg
     2581 gtttgccgta caacttctcc acggtgactt ggtgattcct cgagtaatgg gtgatcgttt
     2641 acagatccat ccgattacga tcttaatcgt tttacttgtc atgggtgatc taatgggaat
     2701 tgtgggcgtt atttttggta ttcctatcta tacgttagtc aaattactcg ttacttttat
     2761 tttccgtaaa ttcaaacaac gctacaataa attttatggc gataaaggag agtatgaaaa
     2821 ctcttcattt tctgaagaag attattttaa ataaaacaca aaacgtcgat cttacgagaa
     2881 tagctcgtag gaacgacgtt ttgtgttttt gttttaattt ttcagcaaag caattgcctg
     2941 gtatggtttt agctccagac tttgatgaag aacagtatcg gaataattat cgataagaat
     3001 attggcttct gtgaatcctt ctgggatggc gatccttgta tctttatcat agaaatttgt
     3061 taatacaagc agctgctgtc cttcaaattg gcgaagaaag ccataaactt ccggatgatt
     3121 tgcttcataa gcatgataat ctccttgtgc aacgattgga tatgtttttc tcagccgaat
     3181 caatttcttg taataggaca aaatagaatc ttcagaagat agttcttttt ctacattaat
     3241 ctcttctagc ttgcctccaa cttttaacca aggtttccct gtagtaaatc ctgcttgttc
     3301 tgtgtctgac cattgcatgg gtgttcgaga gttatcgcga gattttgctt tgatccgacg
     3361 aaacgcttct ttttctgtga atccttgttc aaggagtaac ttataagcat tcaaactttc
     3421 tacgtcaata tagtcggaaa tcgatgaata gtctgggtcg atcatgccga tttcttctcc
     3481 catataaata tagggcgtcc cacggttcaa atggatcatg gttgctagca tctctgctcc
     3541 ttgttttcga tactgatcga tagctacaaa tcgattgagc gcacgtggct gatcatgatt
     3601 attaaaaaat agagcattcc agccgttacc ctcactcatc ttttctcccc atgtatgaaa
     3661 tagtcgcttt aattctgcaa aatcaaatgg aggagtagac catttattcc cattttcata
     3721 atcgactttc aaatgatgga aattaaagac cattgataat tcttctctac taggttctgt
     3781 atacaagata caattttcga tcgtcgtaga gctcatttca ccgactgtga tgatagtgga
     3841 atccgtccca aacgttgctt ggttcaattc ttttagatac gtatgggtga ttggtttgtc
     3901 cgtatattca ggtttgccat cattttcttt attatttttc aattgttcgt ctttaccgat
     3961 aacattgata acatcgaaac ggaatccttt tacccctttt tccatccaaa aacgaacaac
     4021 tttgaatagt tcctctctta ctgcttggtt ccgccagttc aagtcagctt gtgtccgatc
     4081 atataagtgc aaataatatt tccccgtatc tccaaatggc gaccaagcac ttccaccaaa
     4141 ttttgatacc caatccgttg gctcttctct taaaatgaag aagtcctgat aatgctggtc
     4201 ccctgctagt gcttttttga accattcatg ttctgtcgaa acgtgattca gcaccatatc
     4261 gagcatgatt tctatccggt atttctttgc ttcggctatt agctgttcga attcctccat
     4321 cgtaccaaag ataggatcga tggctgtgta atcagagata tcataaccgt tgtctttttg
     4381 agggcttgga taaatagggt ttagccaaat catgtcgatg cctagatcat tcagatacgg
     4441 gagtttttct ttgattcctt ttaaatctcc aattccatca ccattactgt ctttgtaaga
     4501 tttgggatag atttggtaaa tgactttgtc tttaaatgtc atggatattt acctccattc
     4561 aactgattta ttacctacca caacttatat atacaagtta tcatttatta ttataactga
     4621 accgaaagcg tttgtaaaat ttatctttat acattctaag aggtctgttg cccaataaaa
     4681 aacaagagat cagacatgta tttgtctatc ctcttgttta aaatagcaaa atattcttgt
     4741 ttcttctata aatggtgttc aaaagctttt atctacggca atcagtcttc tcaaatgtaa
     4801 aacatgagga gctaatttta tttgagtatt ctttttgctc gatgcaggac ggctttttta
     4861 caacctctga ttcatggtgt tcaatcagct gtccttcggt attagctcaa aaaacggcaa
     4921 aatatgaaaa actattttgc cgtttgtttc tctaatactc aggtcaaacc gctgatttca
     4981 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5041 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5101 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5161 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5221 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5281 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5341 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5401 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5461 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5521 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5581 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5641 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5701 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5761 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5821 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5881 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     5941 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6001 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6061 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6121 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6181 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6241 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6301 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6361 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6421 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6481 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6541 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6601 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6661 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6721 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6781 ttgagataat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6841 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6901 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     6961 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7021 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7081 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7141 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7201 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7261 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7321 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7381 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7441 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7501 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7561 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7621 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7681 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7741 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7801 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7861 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7921 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     7981 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8041 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8101 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8161 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8221 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8281 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8341 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8401 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8461 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8521 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8581 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8641 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8701 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8761 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8821 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8881 aggattcttt gaaagataca gataatatgt tgactatcac aaaatacaat ccaagtgctt
     8941 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9001 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9061 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9121 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9181 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9241 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9301 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9361 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9421 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9481 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9541 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9601 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9661 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9721 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9781 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9841 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9901 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
     9961 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10021 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10081 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10141 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10201 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10261 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10321 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10381 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10441 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10501 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10561 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10621 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10681 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10741 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10801 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10861 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10921 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    10981 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11041 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11101 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11161 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11221 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11281 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11341 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11401 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11461 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11521 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11581 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11641 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11701 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11761 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11821 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11881 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    11941 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12001 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12061 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12121 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12181 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12241 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12301 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12361 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12421 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12481 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12541 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12601 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12661 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12721 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12781 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12841 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12901 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    12961 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13021 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13081 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13141 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13201 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13261 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13321 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13381 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13441 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13501 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13561 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13621 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13681 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13741 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13801 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13861 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13921 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    13981 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14041 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14101 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14161 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14221 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14281 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14341 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14401 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14461 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14521 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14581 ccaacctggc gacctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14641 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14701 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14761 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14821 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14881 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    14941 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15001 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15061 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15121 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15181 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15241 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15301 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15361 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15421 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15481 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15541 agctgcaact gttcctgtac taaccttgtt aaggttagac aaatcattct ggcttgatct
    15601 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15661 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15721 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15781 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15841 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15901 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    15961 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16021 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16081 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16141 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16201 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16261 gcgtataccg tacttctaca tctaaagttc caggaaatcc gttttccata tctgggctag
    16321 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16381 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16441 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16501 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16561 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16621 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16681 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16741 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16801 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16861 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16921 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    16981 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17041 agatgaaata caatacgaag aatatcgtaa acggaaccca aatatctttg tatggatttt
    17101 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17161 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17221 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17281 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17341 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17401 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17461 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17521 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17581 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17641 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17701 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17761 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17821 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17881 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    17941 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18001 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18061 tgaaataagt tcctaatacg atcgtaacta aggctacccc tataccaaag acttgatagg
    18121 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18181 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18241 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18301 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18361 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18421 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18481 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18541 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18601 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18661 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18721 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18781 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18841 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18901 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    18961 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19021 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19081 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19141 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19201 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19261 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19321 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19381 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19441 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19501 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19561 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19621 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19681 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19741 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19801 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19861 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19921 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    19981 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20041 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20101 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20161 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20221 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20281 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20341 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20401 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20461 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20521 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20581 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20641 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20701 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20761 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20821 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20881 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    20941 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21001 ataaatatct aatgttactt ttttaatttt tttttcgaat acgcgccatt acaccttcta
    21061 gttctaactc tttgtcttca gtgatgtacc aaccgcttgc ctgtgtccct ccggctgcct
    21121 gcggtaaata atccaatttt gttcgaaccc agtgcttttc ttctgtaaaa tagtcttcta
    21181 ccaagacctc tagatccatc cctgctgaaa gaggcaaatc ttcccgaaca gatttaaaga
    21241 taaatcgttc cttcttccca tcatacttca atattcctgt atcgataatc ggtaaagaat
    21301 aataatccag ctctttcttc accgtcaata aatgtttgaa aacttcatgg tacacttgct
    21361 ctaattggcg accttctgaa ttgttcgggt aattaaccat cactttttct ttgtctggat
    21421 catagaaggt ttggcttagt tgagtgatat aagtatgtaa tctttccatt tctgttttta
    21481 acttttctct ttgaccttct tctaccttca tatactcttt caccctgttt tctttttaga
    21541 tattttcttt cttattttac tatagttatg tgcgttaagc gatgaaaaac agcaaagatt
    21601 ggaaggatct ttgctgtttt ttcttaaaat atccagttga gtacaggatc aatctgtttc
    21661 attgtcctct tgatccgtcg taatattgat cgatgtgtct ttagcagtga tctttagctt
    21721 tttctgtcct tgttctaaca aagtatttgt tattggctgg tagtcttttt caatataaga
    21781 ttcaccagac gtttggatag tatagccact gttttccatc tcatgcaaag ataaattgcc
    21841 accgtttagg atgacttttc cttctttgct gatcaaactg tcttctagat ggaagttgct
    21901 gttttcacct gtgacgatca agttatccac attggcctca tctaatcgaa gtgagcctaa
    21961 ttcatctttc gcagttattg ttcctcctgt aaattcattg aacgataccc agccatcatg
    22021 tgtcttagta ttcacttttt tgattgatac ttcgttgata tcaattgcac tattattccc
    22081 ttcgattgtc aattcagata attcatgatc tgcagggaca acaatagtga tttgtgcact
    22141 tgtgtcttct aaactaatga ttgcacctgt tgattttttg actgcattca cagtcaacac
    22201 gccatttttc tcctttattt ctggcttttc tgtcttatca taagtcgttt ttatctcaaa
    22261 atccaatccg cgtatgaccg aaactgtttg attttctgca tggatgacga ttttgtttgc
    22321 atccttgatt ttttttgtct cagtaaccat atcgatcaac cgaggaccat tatcccaagt
    22381 cagactcttt tgagcgccta atgtataagt tgcacctgct aggaggccac cagtaagcat
    22441 cagaccaata ccggtaatca aaaaatgttt caatctcaat ttatgtgtcc tccttcaaac
    22501 gcccgctgaa tgcagatctc tttttgtaat aaaacgtcgt cccatccatt taacaaagtt
    22561 catcatgaca taaaaaagcc cttttgttcc tttcagcaac agtggcgaaa taaatactgc
    22621 gcttcctgtg gcgaacagac cgacaccgat aaagaataat ccactcagaa tcgattgtcc
    22681 aaacacactg attcctgaga tgatcatgaa taggccaaca cctaagatac tgatacataa
    22741 aacatagata ctaaaaatga gcgctagtaa agcaaccaat agtcccccaa taaccaatat
    22801 aaaccccagt gctagtggaa tgaggacagg agaagcaaat aaaccgataa tgattaacca
    22861 aatcaggcga atttgtcttt ttggattttt cactaactcg ttttctgctt gttcttcttc
    22921 tgaaaagtaa ctgattttta attttaaggc aaactgcttt ggtgtaccat attcatcaat
    22981 gatctgatct aatgtttttc cagaatctaa aaaatattcc tcataatact ggatcaaatc
    23041 aaaacgatcg tcttcaggaa tttcttgaag atgatccaca acttcattca aatattcatt
    23101 catttgtgct ctcctccaat aaccggtcta aagaatctcg aaaaacaatc cattcatcaa
    23161 taatttcttc taaatgacga cttcccattg gtgtcaacga gtaatatcga cggtttctgc
    23221 cttcaaaagg ttcatcatag gtctcaagat atccttcttt tttcaatctt ctcaaaaccg
    23281 gatatagtgt agattcactg acagagagac tttcttgtac gatttttgtt aaggcatacc
    23341 catacatatc ttccttgctt aatgctgcta gcacagtacc atcaagaaga acagttggaa
    23401 tctggatgtg cattacgtca ccttctattc tcaagtgtaa tattttattt caatctatat
    23461 tatacaacat ataatatagt ttcaaaatca gtttgtcaaa tacaaaaaaa ctgactaaaa
    23521 gatttgatcc tttttagtca gcaaaaaatg gcttctcttt gatttttatt ataacagtgg
    23581 cataatccaa tttgtccaca tccagctcag tggcatgata acgatcattg caggtatcat
    23641 gtcagctact gggaacattt tcacgttgat cattctaaaa ccggtagcga ccatcaagaa
    23701 tccgccgcaa gctttaaaat ctaagatcat gtctggtgta gtcaatggat aaataaattt
    23761 tgccaataag aacaaaatat aaaaaataat gaattgggga attgctatca cagaaacgac
    23821 ataccctaaa ttacacgcaa aaatagctgc tgtgaaaaaa tcaaggacag atttagaaat
    23881 caaaatcgta ctgtctcctg tcatcccaga atctaaactt ccatagatcc ccgtaccact
    23941 tgcacaaaat agaacaatga ccgtcactaa tgtgctgata aattcatcgt gggtcatacc
    24001 aagcttttca ctagggaaga ttttagaaat aggtacttgc atcagcgcag cccccttatt
    24061 tatcccatta cctaaatgaa caatcaaacc aatcgctgtt cctatcacta aagcaaaaat
    24121 aacagccggc atatttttca tcggcgcaat ggaatagatt cccatcccca tcgaacacac
    24181 accaaaaacc atcgtcagct gtgctttaaa atgttcactt aatttatttc ctactaatcc
    24241 gccaattagt ccgccaaaca aaacggaacc tacattgatc aaaactccag ttggcatcaa
    24301 aaaactcctc tctttttcta cgaagcgaaa aaaatcagaa aaatttctgt aattattgct
    24361 ttcgctgcaa gaaagattgt aacagacaga gaagattaca ccaattcatt ttttgtgtta
    24421 aagtattctt tattggaata agtaggagga atatgatgga tatccataaa ttcgaagttt
    24481 ttcttgattt agccgaaaca atgaatttca ccaaaactgc tgatcgacaa ttcaccaccc
    24541 aaggaaatat ttctaaacag attctttcgt tagaaacaga attagacgtc aaattattcg
    24601 aacgcgccca tagaaaaatc gaattgaccg aagctggaag cttattgctc ccatatgtaa
    24661 aaaatgtcgt agaacaatac catgctatgc aagaagtgct tgaaacctat acgaaagaca
    24721 acaatctttc attaaatatc ttaacgatac cgacaatgat caattataaa ggtttttcca
    24781 aaatcaccga gtttttgaaa cgccatcctg aatttacagt ccagttgaaa gaagtggaaa
    24841 gtgtcgaatt aacttacaat gacgccaatg aacatagtga cacgatccac tttgctagaa
    24901 gtttccaggc cccaccaagt tcgattgagt ggttgcctac tgaaacagac gattttgtag
    24961 cagttctacc taaaaatcat cctttagcta gtaaaaagaa attggatcta agcgaactga
    25021 aaaaggagaa ctttttgctc cttggcccca ctacgaactt gtatcaacct gtactagatc
    25081 tgtgtcgcga agctggattt gaaccaaaaa tctcttataa aggagcaaga atcgacttga
    25141 tcatcaatat gattgcaaat gacatgggga ttgctattgt tatggaaaaa acagtcaaga
    25201 atctattaaa agaaaatact gtgattctcc ctatttctcc aacaacagag agttatcttg
    25261 catttactcg taaagtcggt gagcattctt tggcttcaga tacattttgg gagtatttaa
    25321 atcaagaaga aaactaagcc ctacattttc tgtccatcta tccacccaaa aaaagagagc
    25381 gacgcatgac tttcgctctc tttcttgcat catgtccttg tttcaatgac acctctaaca
    25441 tttctcgtag ttcaataaga aagcagcgat ttttatgcca aaagaaccgt aagaacattt
    25501 ccctaatcac aaaaaaacga ctaaaaaccc ctaaaaacct tcaatagaat ttttaggggt
    25561 tttattcttt ttcctattgt ttacttaacc gtatcattgc tgtcgttcct actccttcga
    25621 tactgctata atcaatacgt ccatcatgca gttctagaat ttttttaacc agtgaaagtc
    25681 ctagcccatt cccagaaatt tgacgacttg tatctccttg ataaaatttt tcaaacattc
    25741 gatctctggt ctcttcattc attcctacac ctgaatcggt cacttccagt acaatctctg
    25801 tggctgtctc aaatagtttg atatgaatct gtccattgat ttgattgtac tttatggcgt
    25861 tatccatgat attcagccac acttggtaca aaaactcttc attgcctgtg taatttactg
    25921 cagcaagttc gatatccaat tctagctgtt ctttctccca tttaggctga aaaaaaacga
    25981 ttacttctct gatttgttca tctaatcgat actctttctt ttccagttgg atacgctggt
    26041 tttccagttt gttcaatttt aagatgttct ctgttaattg tgaaagctgc gtgatacttt
    26101 cgataatccg atataaaaat atttggcgtt cttcgtcaga gagatttggg gcttgtaaca
    26161 attgaacata tccttggatt gttgctagtg gtgttttgaa ttcatgggat acactggaca
    26221 caaagtcatt cctcagtgtc tcgatactat tgagttcttg gaccataacg ttgaaatctt
    26281 tatacagttg ctgtacttct gctactttct gctgttcatc cattcgaatc gaaaaatctc
    26341 ccgttgctac cagactcata tttgtcccta gcgcactgat tggatgcagg atacgccgcc
    26401 caacaaaaat cgatattcct gtacccacga ccatactgaa ccccgcaaag acgaataacg
    26461 ggtataacgt tggttttcca gccgattcag caggcgagat agaatgtcga gacaataaga
    26521 aaataaaacc gagaaaacac agcagtgtca ctaagatcga aatgaagaca atactagcaa
    26581 aatagatcca taattgtgaa aatagctttt ttttcatctt taaataaccg ccttatagcc
    26641 tagtccccgt actgtgatga tttgaaaatc ttccgtcttt tcaaaacggg tccttaaacg
    26701 tttgatatgt acatcgatcg ttcgttcttc tgtattcgta tctaatcccc aaatatcatc
    26761 catcaactgt tggcgagtga agatcttgtt gggataagac agtaatttat acagaagcag
    26821 aaattctttt tgaggcagaa tcaacgaatg ctgatcttgt aacactgtat aagcatctag
    26881 ctctaatttt gtttgtccga ttatcaagct actgctgtaa ttgatctttg aacgtcgaag
    26941 caatgcttcc acacgtagga tcatctcatt gacatcgatg ggttttgtca tataatcatc
    27001 tgctcctaat ttgaacccgc gtttcttatc ctcaaaatcg gcctttgccg taatcattag
    27061 aacaggtgtg tcaaatctgg tgctacgtaa tgagtctagc aagtcatagc catccatttt
    27121 cggcatcatg atatctgtaa tgattaattc tatacgtgtc gcttctagtt ttttcagcgc
    27181 atcctctcca ttcaatgcgg tgatgactga aaagcccgct cgttccaaca cagaacggta
    27241 aagcaactgt aattgcggat catcttctac tactaaaata cgattcattg atctccctct
    27301 gttttttgaa aaatttctag attttttctt gtagttcatc ttcagttaac atttatttcg
    27361 tatactacga tcattgttct gatatactta ttagtatacc gaatatttca atacgggaga
    27421 agggtgatta tgcttcaatt aaaagatata aaaaaatatt ataaagtcgg cgaaaccacc
    27481 accaaagcat tggatggtgt gtctgtcgct ttcagacaaa aagaattcgt agcgatttta
    27541 ggtcctagtg gttctggtaa aacgactatg ctgaatgtga ttggtggact agataattat
    27601 gattccggtg atatggtaat caacggaaag tctactaaag actttaagga cagtgactgg
    27661 gatgcctacc gaaacaactc tatcggtttc atttttcaaa gttacaactt gattggtcat
    27721 ctaggcatta ttgaaaacgt tgaacttggg atgacattaa gtggtgtttc taaagacgaa
    27781 aaacgaaaac gggcagaaga tgcattgcac cgagtcggct tgacaaatca tatgcataaa
    27841 aagccaaatc agttatctgg tggacaaatg cagcgagtgg ccatcgctcg agcactagcc
    27901 aatgacccag atatcctttt gtgcgatgaa ccaaccggag cgttagatac agaaacaagt
    27961 atccagatca tggaattgat ccaagagtta tcgaaagaaa aattagtgat catggtcaca
    28021 cataatcctg aactggccca tcaatacgca gatcgaatca ttgaattttc tgatggtaag
    28081 atcttaacag attcccatcc tcacatcgaa cgaccaaaag atgatcaatt taacttacgc
    28141 agaacaaaga tgagtttttg gacagcgtta aagctttcct tcaacaatat ccgaacaaaa
    28201 aaaggacgta ctttcttgac ctcctttgct tccagtattg ggatcatcgg aatcgcgatc
    28261 gttctgtccc tttcttctgg attccaaaaa caaatcgata acacgcaagc tgaaacgatg
    28321 gcgaaatttc cgattactat ttctaaggta acgactaacc agactcgtga cgatgccgga
    28381 ttgggtgcaa gcaaagcgga ttatccagac agcaaaacga ttactgcaaa agtcagcgaa
    28441 gaagataaag cacagcacac gaataagatt gatcaaacat acgtcgatta cgtaacagac
    28501 atcgatccta acttaagtaa taatatcgga tttacccgta ctacaggaat caatctttta
    28561 cgtgatgtaa atggcaaagt acagcctgtc agcttctcga atcaaaatcc tgatacggaa
    28621 tctctttctt tatctagtac gatgtcagca atgacaggtg tgggagtgtc aagtttccct
    28681 acacagcttg acactagcaa agaaaacttt ttaaaagaca attattcttt acttgccgga
    28741 agttatccag cctcagcaac agatgtcgtg ctgatcgtag acggcaataa taatactaat
    28801 atcaacgcat tgaagaatct tggatttgat gtgaaagaag acgaaaaact tgattttgat
    28861 gatatcgttg gtacaacatt caaattagta aacaacaata cttactatac aaaacttcca
    28921 actgggaatt ttatccctaa tacagattat gatgcgatgt atcagaatgc ttctgatgaa
    28981 ttgaagatct cggggatcct acgcgtgaaa tcttcttcta caatgaatct cttatctcca
    29041 ggaatcgctt atagcgatca attaacgact caaatcgtca acgaaaacaa agaatcagaa
    29101 atcgtcaaag ctcaaagaga cagtggtgta aatgtcttaa ctactgaaaa agttgacgaa
    29161 aatgccaaac aaacattgct tagttattta ggtggagaca gcttgccttc aagtatcatg
    29221 atttatccga acaattttga agacaaagaa aagatcttgg attatctaga tgattacaat
    29281 aaaggcaaat cagatgaaga taaaatcatt tatactgatt tagctggaac gatgactgaa
    29341 ctgactggcg gattgatgga tgctatcact tatgtcctta ttgcttttgc agggatctct
    29401 ttagtaacaa gtatgatcat gatcagtatc attacttata catctgtgat tgaacggaca
    29461 aaagaaatcg gtgtactaaa agctttaggt gcacgtaaaa aagacatcac tcgcgtattt
    29521 gacgcagaaa cgtgtatttt aggtatttct tctgggatat taggtgtgtt tattgcatgg
    29581 ctagctacgt tccctatcaa tagcatctta tacaacatga cggatctaaa aaatgttgct
    29641 caactaaatc ctgtccatgc gattattcta gtgatcgtct ctactatatt gacgatgctt
    29701 ggcggtcatc ttcctgcacg aatggcagcg aaaaaagacg ccgctatcgc tttgcgtgca
    29761 gaataaggtt gtgacaacca catttagatt tgagtcataa gagcgctgaa atagtagaaa
    29821 acagcaagaa acgaggaagt aatatgttcc ttgtttcgcg ctgttttttt taattatttt
    29881 ttataattct ggtatacttt tctttccttt gtaccacaac tcttctggcg tatacctgtc
    29941 ttttccattt atgaatactg ttccatctgc ttcaatcgtt acttcagtcc caatccacat
    30001 attacgagaa agtgcgagaa gattttttct ttttcctctt cctttttttc caaattgcaa
    30061 ggcatcggtt atctcttctg tccaatgaga tttcacgtat tttacttctc cgttttcaaa
    30121 tgtaactttc aaaatattcg ctatattttc cacttttact gctttaggaa atgactcctc
    30181 catttttttc actttccttc ctccaacact aagtatgcgc tttcttttag tgataagtat
    30241 atcatagttt attctattat caaaaaaacg catccattcc attgtagaat actttaccga
    30301 acaagttgaa ttgttttcct ctttttcacc ttttactata gataggtaaa gacaactaag
    30361 gaggaatctc tcatgacaga tcaaccgtat gatttaagaa aagtcatcga agaactaaaa
    30421 acacttccag gtcaatatca cgaaacagat attgaaatcg atcctgaagc agaattatct
    30481 ggtgtttacc gttatatcgg tgctggcggt acagtaaaac gtccgactca agaaggacca
    30541 gctatgactt tcaacaatat caaaggtttc cccggcacac gtgtgaacat cggaacaatg
    30601 gctagtcgta aacgtgtagg tgaaattttg caccatgatt atcgaacact tggtcgtttc
    30661 ttgaaagacg ctgtagaaaa tccaatcaaa cctgtgaaag tagacaaagc agacgctcca
    30721 gcacaagaag tcgttcatct agctaccgat gcagacttcg atattcgcaa cattttgcct
    30781 gcacctacaa atactcctga agatgctggt ccatacatca caatgggtgt agtcttagga
    30841 tcagatccag aaaaaacaat gagcgatgta acgatccatc gtatggttct agaagataaa
    30901 gatacgatcg gtatgtacat catgcctggc ggacgccaca ttggtcactt ccaaaaacaa
    30961 ttcgaagcct tgaataaacc aatgcctatc actatcaata ttggtttaga tccagcaatt
    31021 gccattggca cgactttcga accacctacc actccacttg gctacaatga actatggtgt
    31081 gcaggagctt tgcgtaacga acctattcaa atcgttgatg gtatagcagt cgatgaagtg
    31141 ggtattgctc gtgctgaatt catcattgaa gcagaaatca tgccaaatga aacaatgcaa
    31201 gaagatatca acacgaactc aggaaaagca atgccagaat ttcctggcta tactggggat
    31261 gctaatccag ctgtacaagt agtgaaagtc aaagcagtta ctcaccgaaa agacaatcca
    31321 atcatgcaaa caacgattgg tcccagtgaa gaacacgtat ctatggccgg tattcctaca
    31381 gaagcaagta ttcttgcctt ggtcgataaa gctattccag gaaaagtagt aaatgtctac
    31441 aatcctcctg caggtggtgg taaattgatg accatcatgc aaatccgtaa agaaaacgaa
    31501 gcggatgaag gtattcagcg tcaagcagct attcttgctt tatcagcctt caaagaattg
    31561 aagacagtca ttttagtcga tgaagatgtt gatattttcg acatgaacga tgtagtttgg
    31621 actttcaaca ctcgtttcca aggagataaa gatatcatcg tgatcccagg aatgaggaac
    31681 catccattgg acccatcaga attaccagaa tataatcctg gctctattcg tttccgtggg
    31741 ataagtgcaa aaactatctt tgacggtaca gttcctttcg atatgaaaga acattttgta
    31801 cgtgcaaaat tccaagaagt agaagattgg gaaaaatatt tgaaataatg aatgatctgc
    31861 aactgttttg acatcataaa aaggagtgcg gaaaatggaa caattggcac aacggaagaa
    31921 aaaaatcgtg attggtgtaa gtggcgcttc aggcaccatt tatgccatca acctagtgaa
    31981 gaaactaaaa gaatatccga tgatagaaac acacggaatc atcagtccat gggcaaaaca
    32041 gaacttaaag ctggaatccg acttatcact aactgaattc gaaagcctgt ttgattacca
    32101 ttattcaaac aaagatttag gtgctgctgt cgccagcgga tcatttttga ccgatggaat
    32161 gatcatcgta ccggcaagta tgaaaacagt cgcaggtatc tctgttggtt taggcgacaa
    32221 cctgattgca cgtgctgctg atgtaacaat gaaggagcaa cgaaagttag tcattgttcc
    32281 tcgtgaaacg ccattaaatg ccattcactt ggaaaacttg actaaattag cacgactagg
    32341 tgtgcaaatc ataccaccaa ttcctgcttt ttataatcag cctcaaacga tccaagatct
    32401 tgttaatcac catacaatga aacttttgga tcaatttgaa attgtaaatg agcattcaaa
    32461 acgttggcag ggtgattaat atggaaaaag aaacgatagt tgaagtaacc aaactaggtt
    32521 tcacgatcca agctgctgtt aaagtaatcg gtaaagactt attgatcact ttaacaggcg
    32581 gagatacacc acatatcgga acagtcacta cattttctcg tgatacagag atgcaaactg
    32641 tacgtttccc tagccacgat ggtcggttcc ataaagacga tgtcttagcc gaaaaaatag
    32701 ctgtgatcat caaaactgcc gttccgggaa gttgtacgat cacttccggt atccatgttg
    32761 accatatctc caatgcacaa atcaacgcct cttttccgat gtcagaagag ttaggttaca
    32821 agattttaga ttggttggaa aaaatagatt tccatatcaa ataaccagtt tattataaag
    32881 atggtgaaaa accattatga aagaagaggt tgtgaaatca gcgttatgac ctgagtatga
    32941 gagaaaaaaa tggaaaaata gcttctcata ttttgccgtt tttgagctaa taccgaggga
    33001 cagctgattg aacgccgttt atcaagaggt tgtgataaag ccgcttcgtt tcgagtagta
    33061 agaaaaattt tgtaaacatg ggtttccaca tttttgcata aatttgactt actaccgaga
    33121 aactggcttt tgaacaccgt ttatcaagag gttgtgacac gtcttttgtc acaacctctt
    33181 ttatttttgc ttcactgtaa ctgctacaat atcaccgatt tcttttccga tgtttttacg
    33241 aatatctttt cgtagtccaa ggatgtagca gactgaacca tctggatttt tcacgcccat
    33301 attcacgata ctgccttcat acggcacatt gtcaaaagtt gcttggactt taactcttcc
    33361 agcaccgaat tcttttctta tatcatacgg aaaaataata tatgccccgc ccttgccgac
    33421 ttcggaagca tagatttttg attcaaaaga ataaatttcc ctcattttat ttctcctatt
    33481 ttttacatct agtatagcat agcaaaaaaa agaaggaact gtgacaaaag tcttgtcgca
    33541 gttccttttt tcgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatag
    33601 tcaacaaaca tgagaagctg ttttttgaaa ttgctccttg tgactcagag ttaaacggca
    33661 ggctcacaac ctcttcctta aacggtgttc aaaagcttgc atctacggca ctaagcccaa
    33721 tcagatgaaa aatatggagt gctattttta tctgattgtt cttactgctt gatgcagagc
    33781 agctttttca caacctccta catggtgttc aaaatattat tcgccttttg cttctttcac
    33841 taattttgcg aatgcttgtt gttggaattc agcagctaag gctttcgtac gttgttcttg
    33901 ctctgctttg ctcatttttg gattttcagt cattgctttt accataccgt cttgaacata
    33961 agcttttgct tcactttcca tcgtactttg ctgtttcttc actaattcag ctaacttggc
    34021 tgcttggtcc gtacttaatt ctacccatgt ctctgggatt ttgaatgtat tcactcggct
    34081 gtcatattcg tgattgttat cagcaatatt gaaccagaat ttatacatgt cgtttttgta
    34141 ttcccaataa gtagttttcg tcactttttc agcatcgccg tctgtctttt ctactttatt
    34201 tgtttcgtta tccgtaccag ttgcttttgg tttttcttgt ttttcatttg ttcggtaaag
    34261 atacactttt tcttttccat tccctaaagc tttatacaaa agcatatctg ctccttggga
    34321 atcagcactg gaaacaattt tttctgtttt tgtttccgtt actttttcca ttccaaagtg
    34381 attggacaaa ttagcgacga tcaaacttag tgaagcgacg aagattagtc cgaaaatcaa
    34441 agataaaaag gtttgccatg tttttttagc gaacacgttc gttaaagcaa aagcaagcaa
    34501 gctgacaatc aaaactccaa taatcatgca ttatcccccg ctttcattag aacaggttct
    34561 tttttcttca ataaaaaggc aataataaag ccaagaatcc caaagatgat cgctacgata
    34621 aacgcggcat ggtaacctga cagtgtggca ttgattgcct gatctttata agctaatggc
    34681 gtattatcca atacagattt gcttgggata tttgcttttg tcacattcgt caacacactg
    34741 atcaaaatag ctgtaccaat cgagcttgcg atttgacgga atgtattatt taccgctgtc
    34801 ccatgactga tcaagttatt tggcaatgca ttcataccag aagtcgttac cggcatcatg
    34861 accatggaga tcccaaacat acggatcgca taaaggatca cgatatataa aactggagtc
    34921 gtttttgtca agaaagcaaa tggtgcagtt gccgctgtca aaatgaacat ccctgtgatg
    34981 gctaaacgtt tcgcgccgta tttatcgaaa atcgcacccg tcacaggcat catagccccc
    35041 atgatcaatg cacccggcaa caacatcaag ccagaatgga atgctgattc tccacgaata
    35101 ttttgaatat acaatggtac gaccatttct gcccctacca tcgccatatt tgttacacca
    35161 gctaaaacag ctgcaatcgt gaatgtagat gatttgaata cacgaagttc caagaatgga
    35221 tgttccaaat gtaattgacg ccatgagaac aaggcgatga aaatcacacc aacaactaag
    35281 aaaccaatta ctttcaagct tccccagccg tcatctccta cactagaaaa tccgtaaagt
    35341 aaactaccga atccaatcgt tgataaaata gcggataaaa tatcaatgct aggatttgac
    35401 aatggaataa cacttttcat taggaagaat gctaaaacca agacaagaac aacgattggc
    35461 agtaccatac caaacaagtc acgccaatgg tagttatcaa tgatataacc agataatgta
    35521 gggcctaaag caggagcaag tccaataacg atccctgaca tccccattgc tgatcctctt
    35581 ttttctggcg ggaaaataga tagcatgata ttttgcaata atggcatcga tactcccaca
    35641 ccgcaagctt ggatcaaacg accgattagt aatgtactaa aattaggtgc tgtaaagcaa
    35701 gtaatcgttc cgatcaaaaa gatcgtcatg gcagaaagat ataaatttct tgaactgaat
    35761 ttattgatca gccatgctgt tattgggatc atgatcccgt tgactagcaa gaaaccagtc
    35821 gttaaccatt gcacatcaga cgctgaaata tcgaaagctt tcatcaatgt aggaaaagct
    35881 gtcgtcaata aggtctgatt caaaacggta caaaatgtac caattaataa gacaacaact
    35941 aaaagtgtcc gattatatgg ttttccgtaa atatctacgg gttggttgtt actcatattt
    36001 ctccttcttt ctgaattttt tctgaaacta ctagaatact ttagtcctaa attcgctatt
    36061 tgtcaaccta cttgacacat aagttaatac ggatataatt aagggaagca agaggacaaa
    36121 aggagtgaca aaaatggttg gggcttccct taaacgtttg ttggaaaatg atcaattggt
    36181 tgttggcatc agtgaattaa gtgatatcgt aggtgtgtct ccacgtcaat taaggtattg
    36241 ggaacaaaag ggattcattc gttcgatcga agctgatgcg aattgtccta gaaaatatcg
    36301 tttacctaca gtcataaaag tagaactgat caaaaaatat ttagatgacg gatttacttt
    36361 gtcaaaagct gcagaaaagg cagaaatccg ccaaaagaaa atgcaccatg tccgtaaagt
    36421 tttttcaaaa gcaatcaaag atatcgaatt gatcgacgaa cgctatacga ttttacaaat
    36481 cggtaacttc aaagaagaga atgctaagat gtacatcatt catgacaacg agacagacga
    36541 attaacttac aagctccttc ctgtcgatga aaaagctgac tacgaacagt tgttaaaaga
    36601 actctaaacg ccactaatct cctaagtacc aagcttttta ataagtagat gctacataaa
    36661 tacttgaaaa aaggcctgtt tgaacataag aatatccgaa cggtgatctg cgtttctttc
    36721 atatagaaag gaattctcca tcactgttcg gatatttttt tatttttttc ggttactttc
    36781 ttctaataca tctgttagcc aatttgcata ttctgtttcc cgattgatcg catgcgctaa
    36841 aatcaggtag tgtccgtaat tttcatgttt ttctttttca gtaggaaaga tcgttttttt
    36901 tcgctctaat agatgaagtc gtttttcttc atgaaggtca cgctgctgct gaataatttc
    36961 tactaatgcc ggatcattca cgtctttgac gaaatacaat ttcagaacaa attcgtctcg
    37021 attaaccggt agttcattgg tgggtgtatg aatccactct gccaattgtt ccttcccttc
    37081 ttctgttaat tcataaactt tcttttccaa tttttcacct gtgatctcta cttgatgctg
    37141 aatgattcct tgctcttcca tttttgcaag ttcaggataa atctgactat gttttgcttg
    37201 ccaaaattct cctatttcat ttttgaacga ttgattcaat tcatatcctg tcatcttctt
    37261 cttacttaat aaccctaaca aaatatatcg aagtgtattt tttcttgcca tcagttttcc
    37321 ctccttgaca aatctttaaa tccattctat cataaatgag taatcgaaaa catgtagttt
    37381 attacatgtt ttaatttata aggagatgct taaacatgaa agaatttaaa gaattggaag
    37441 attttattgg aactcacttt atctatacgt atgacaatgg ttgggaatat gaatggtatg
    37501 ccaaaaatga ccatactgtt gattaccgta tccatggcgg aatggtcaaa ggccgttggg
    37561 taaaagacca agaagctgat atcgttaaat tgacggacgg cgtattcaaa atcacttgga
    37621 cagagccaac agggacagac gtggcattgg actttatgcc aaatgaaaag aaacttcacg
    37681 gaatgatctt tttccctaaa tgggttgaag aacatccaga aatcactgtt actttccaaa
    37741 acgaacatat cgcagaaatg gaagcagctc gtgaaaaata tgaaacttat ccaaaattac
    37801 ttgttcctga atttgctacg atcacttata tgggtgatgc aggtatagac aatgaagatg
    37861 ttattagcga agaaccatac gatggaatgc cagatgatat ccgtagcgga aaatacttcg
    37921 atgaaaatta taaacgagta aaataaggtt gtgaaagaga ggttgtgaaa tcagcgttct
    37981 gacctgagta ttagagaaag aaacggaaaa atagttcctc atgttttgcc gttttttgag
    38041 ctaataccga gggtcagctg attgaacacc gtttatcaag aggttgtgaa atcagcgtta
    38101 tgctttgagt aataagaacg ctagttagaa aatagctttt catattttct aggctagtgg
    38161 gcttattacc gagaagcagc cgtttgaaca ccgtttatca agaggttgtg aaatcagcgt
    38221 tttgacctga gtattagaga aacaaatgga aaaatagttc ctcatatttt gccgtttttt
    38281 gagctaatac cgagggtcag ctgattgaac accgtttatc aaagattgta acaactgcat
    38341 attcacacag ttaaattagt gacttcaaaa tgaaataatg ctcccagcaa aaaattaggc
    38401 tgggagcatt atttcgttat tctgttcatc aaaaatcagc gttttgattc catttcatca
    38461 caagttcctc ctcatttact gcttcatctt tttgctgtgt ttgaattgta aaaccttgtg
    38521 aaagatagaa atgaatagct gattgatttt tttgatagac tgtcaaagtc agtgttttat
    38581 tggtttcttt tatttttttc attagttgcg taccgattct ttgttgccgg tattcccttt
    38641 tcacaaagat tcctgcgata aactgatcaa tcaccccagc aaaaccaata atttcttgat
    38701 ctatttctgc taagtaaatc actgcatctg gtaaagcttt cctaacagat tctacttgat
    38761 tctcccaata agttgatgga atgaattcat gagaatccaa gttagcatgc agccaaatcg
    38821 atagaacaga ttccagttct ttggcagaca attttgttgt tgtgcggatc attttttctc
    38881 tccttactta ttcattgatt ttaaaataaa aaccattttt ttgaagctct tttacaagtt
    38941 ttcgcccctc aaaaaagtat atcttctctt gttttacttt tctaatacca aaagaaacag
    39001 caactttagg atcatcagct gaaacgattc gataagttcc accaggcaat tgatccagct
    39061 tgactttccc atgatggtcg ctttgtactg aaaacggcac accatttcta gtaagtgttt
    39121 ttttcttatg tttcaaaatg aatgaactac catttaccgg tttccctgac gaatcactaa
    39181 taataaatga aaaatcaagc acttttttac ttaatctata ctgatagatt agtcgtccga
    39241 taaacactaa cagtatgact acagctatta tcgttcctgc aagaataaac cattgccgca
    39301 tggttctttc ctgatttcct tttccttctt ctttttttac cgattcgtta taaggaatcc
    39361 gatgtcctgt cactagcaaa cgatgcgtgt tgatcatata cggcgtacat gtaagcaagg
    39421 taactaagtc ttctccttct gaaatcgtta gaacagacgt gtcatctggc agtaccactt
    39481 gtatattttt aacttgataa gccaatttct cccccaaaac agtcaaaata aacgtgtctc
    39541 cgttttctaa acgatctaat tcggtaaata accgtcgatt aggtaaaccg ctgtgagcag
    39601 agatcactga atgcgtcgaa tttccaccaa gcgggaaaga ggttccttgc aagacgcctg
    39661 ctccattttc aaggatccta ccattgagcc gatcataaag tgaaatattt aattggatct
    39721 taggtatatt gatacttccg atcagccatt cctctaattg gctggatgtt gtactttcat
    39781 ttctgttctt ttcattgaaa ggatcagtac caggattgaa tccttggtca gttaatcgtt
    39841 ggtttttctc ctccattttc tttcgctgca cttcaatttc agctgcggtc cgtttttgta
    39901 cttgttcgat acgtttttga tcaataaaac tatttaaaga atccacataa aatggatata
    39961 aagcgatcag taaccctacg acgatacata ttaataaaaa caaacgaaac aaaagtcgac
    40021 gagactgttt ttgcttactc tgcttcacat gtgtcctccc ttgcaagttt ttttgttctt
    40081 tcttttatta tatgggttca ggaaaaaaat agaaaatgaa agccgatgag gttgtgaaaa
    40141 agctgtcctg catcaagtaa taagtacagc ccaaaaaaat agctactcat attttttgtt
    40201 gggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa gaggttgtga
    40261 aaactgcgtt atgctttgag taataagagc gctagtcaga aaatagcttc tcatattttc
    40321 taggctagtg agcttattac cgagaagcag ctgattgaac accgtttacc gagaggtcat
    40381 gaaaaagcta ctttgtttcg agtattaaga aaaattctgt ttcaaaacaa ggagatgcaa
    40441 caaaacttat gtcgcatcct ctctccttag aactttcact tatgtttttc ttgtaaaatt
    40501 cataaatatt ttcatgaatt ttctaatttc ttgcctacct ctcgcatagg atatatgatg
    40561 aatttatcta aagtttgcaa aggagttcga caattgaaaa aatatggccc atttttagca
    40621 atttttttca ttgctttggc atttctcatg cctcatttat tcacacataa actaattttg
    40681 ggtgctgatg ccccattcca ttacaatcgg ttctatgatg ccgcgcaaca aattaaatat
    40741 ggggatttcc attattttcc ttccctttat ggattccagc aaagtggcag gatcgtgaat
    40801 gccgtttacg gcccattttt cgcttattta caaggattga tcgtgttgct tgcaggtagc
    40861 tggtttcatt atcagattct ttcaaatttc attctttatc ttatttcagg attttctatg
    40921 ctgattttac tgagatatgt caatgttcga aattgggtaa gtgtaccagt agccgtttta
    40981 ttcatgagta cgtattctat tcaatactgg tctatcaacc aaggattcac tagctggggg
    41041 acttgttttt atcctttatg tatgatccct attgctgatt tcgtccttca taaaaagttc
    41101 cctgttatca aagtcgctgt ctcagtggga cttatgacac agatccattt actgtcttcg
    41161 atcatgttag ctttgatgta tatccctttt tatctttatt acttttttaa ccagactgaa
    41221 aaaggaaaag cactgcttca actgattaag tcggtcttgc tttattttgt tctgacagca
    41281 aatatttggg caggattagc tattgtttac agtgggaata agattcttcc tccctttgtt
    41341 aacagagaaa tgagtcaaaa ggcgatagat ctggatggca gttattggct ccattaccct
    41401 aaggcctttc ctatattgat attatcaggt tttttgcttt ttattttctt tcaacaaaag
    41461 aacaccttat tccaaaaaat cgtgttgtac acaagtcttg ttttcttttt tctttctact
    41521 agcttgattc catggacgac attgatcgaa aaaaatgtgc cttttattag tttgatccag
    41581 tttccatttc gatttttcgt gccatttacg ctattgcttc tactttacct tgccctttca
    41641 ctgaaccagt ggtctgaaca aaagtggttc cgtcttttaa gtatatttgg cattcttatt
    41701 ttttcagcac agacgatcca gaacttatac cagcatctgg aaaagtggga aaatgaaaca
    41761 tttgtcagtc gacatactta tctattcgat acgccggaag aagcaaggaa aacattcttc
    41821 agcaaagata tgtctaccag cttgttcgtt ttccaaaaaa ccacacctga ctatctgcca
    41881 atctataagg agacaaaaga caataaatac gaccggtata acgaagaaat actagaaaac
    41941 gaaacaactt ttataaaaac acatccaggc ggtcttttga caatcaagtg gaaaaacact
    42001 ggggacaaaa ctgttcatat ccctgttatc gtctacgatc ggactgtgct acagcaaaat
    42061 ggaaaaacat tgactgatta tgaagtaaca gatatcggga ccccaatcgt gaagcagcaa
    42121 aaaggaatca acgagctcac gcttcattat caaacgccaa tctatttcta tttcattctt
    42181 tctcttactt tgattggctg gtttaccttg ctatgtttat ttatttatca tagatacaag
    42241 cttttacgtg cttaggcaaa agggataaat acatccgatc aaaaaactct ccaaacggta
    42301 taatcgtttg gagagtttta ttttaatcag tttttttatg ttatatgtta tattttttat
    42361 tcgttcacat ctaattcgat ttccaccccg tagtgatcac tgatgattgg actatcgatc
    42421 ccatcaaaaa tccgttgata ttttttaacc tgcattcctt cagggacgaa aataaaatct
    42481 atacgcaatt tttctgtatt ttgttcccaa ccatctattt tcttttccac agtagctgct
    42541 tttccttttt cattcgcgac aacatacgaa tcttgaagaa gaaggcaact ttctcttaca
    42601 gtataataac cttgagagtc tactggatta ttgaaatcac ctaggaaaaa aagtggtacg
    42661 cgagtttcca aaaagtagtt ttccaagttt ttccactcaa aataaaagcc acttttttta
    42721 tctgtccacc aagaaaagtg gcagctcgag acaaaaatgt cctgatcatc tatctttgtt
    42781 ttcccaatca agatcttgcg tgtctgataa tcttctttct tttgaaattg ggagacggta
    42841 aggacctgac tttccaatgt ttttttcgta agcagtgcat ttccttcttc atatttatca
    42901 taaccgatgt ggctcatttc ccaactccaa aaatactctt ttccgtgttc ctttaaaagc
    42961 tgaactaaac ggtaggcaaa attatcttta cggataggta cttgattctt taccggacag
    43021 aacttcatta gcttcgcagg ttctacttct tcagattcca gtaattggtt gatttcttgt
    43081 aaagcaatca cttcataatc ttctaacagg atcttatctg ctaattcttg caattttttc
    43141 tctggttctg gctcaagcca gctatgcgtg ttgagtgtca tgaatttcat tggttttctc
    43201 cttgtcttca tttcgaaaca aaagggttgt gacaaaattt ttgtcacaac ctctccagca
    43261 aatagtgttc tcctgctaac tcctcagtaa taagctcaat ggaccaggag ctattttttg
    43321 tttggctgtt tttatcactt ggtgcagggc agctttttca cgacctctcc actaaacagt
    43381 attcaaactg ctgcttctcg gtaataagcc caaccaaacg aaaaatagga ggagctattt
    43441 ttgtttggct gttcttatga cttgatgcag gacagctttt tcacaacctc tccttacatt
    43501 ttaatggagc cgatcacttt accagctgtc actgtaccta attgcttgat cactacttct
    43561 tctactttat cgtttgtaaa tacgatcatg atcgttggat ctttttcttc gttttggatc
    43621 acatcaagat ccatctcagc taatttcgtt cctgttccta cttcttggcc ttctttgaca
    43681 gcaatctcaa aagctggctg attcatttgg actgtatcga ttcccatatg gatcaaaact
    43741 tcgataccat ctttcgtttc cagcccaatt gcatgtttgc tagggaaaat acttgtcact
    43801 tttccagcga tcggcgcaac aacttctctt gatgttggaa taacagcaaa gccgtcaccc
    43861 atcatttttt gtgagaatac cggatctttt acttcagata aaggaatcac ttctccagaa
    43921 gctgcggaaa ctagttcttg ttctatacct aagtattgct tactacctgc ttgtccattt
    43981 cctgtagaaa ccatttctgg ctctggaatc tctgctcctg attgcagcag atcttcgata
    44041 tctgatttca agacatctgc ttttggtcca taaactgctt ggactccatt gtctttgacg
    44101 atcaatccca tcgcccctgc tcgtttccag gcttcttcag tccctacttt ttctcgatca
    44161 tttacactta cgcgtaaccg agtcatacaa gcatcgacat ctttgatatt ttcttttcct
    44221 cccagcaaat gaatgatctg gaccacttgc tgatctgctg ttcctgttcc gctgttagat
    44281 acaacggttt cttcactgtc attgtcgtaa tttccgttac gtcccggtgt agcaaagttg
    44341 aattttttga tcaagaaatt tgctaagaaa tacgtaacaa caccaaaagc gatcacacac
    44401 aacacgaagt tgataagatc tccacctaga cccgctttga tagctaacgg tgtccgagtc
    44461 agcaattcga tattcccaaa tgaatggacc cgcaatggca agatatctgc catcgcaaat
    44521 gcagcccctt ggatcactga ataaatgaca tataatggca cagcagcaaa cataaacatg
    44581 aactccaatg gttctgttac acctgttaga aagacagcca acgctgctga aaaatacatt
    44641 gatttatatc ttgctttctt atcaggatcg acattacgat acatcgcaaa agccattccc
    44701 atcaagatac ctgaagaacc aatcatttgt cctactttga aacgagccgg cgtccagtta
    44761 tgcaacacaa actcatattg cgacatatcc cctgcacctt taagattcac taaatcagtg
    44821 gcccaagcca accataacgg atcttgccca aatacttgtg tccccgcctg tgcgcctgat
    44881 aaaatctcat acgatccgcc aagctgtgta tagttgatag gaatcgtcag catatgatgc
    44941 aatccaaatg gcaatagtaa acgttctaat gttccaaata aaaacggtgc tagaatcggt
    45001 gcgctttctt gtgattctgc aatccataaa ccaaaattat tgattccagc ctgtacgttt
    45061 ggccacacca cagcaagaat caatgccacg attgttgacc ataagataac aacaaacgga
    45121 acaaagcgct ttccattgaa aaatgacaaa gcatccggca attttctata attatagtac
    45181 ttattataag ccattgcccc gacaaatcca gcgatgatcc ctacgaatac tcccatattc
    45241 aaagctggtg cttccaatac acttgtaaaa aatcctttaa ccatgatctt cgtaccgaac
    45301 agagtgtggg taaatgcatc ttcattcgca agcatgtcac ttgtgacacc aaagatagcc
    45361 ccagtgatcc gattgattaa aataaatgag atcccagctg caaatgctcc gcctgctctt
    45421 tcttttgccc aacttcctcc tattgctaaa gcaaataaaa tatgaagatt accaataatt
    45481 gcccaaccaa tattttcaat tactccgccc gttgtgacaa gcggcgtcca atcaggattg
    45541 atcaagggaa ttgatttccc tatactgatc atcaacccag ctgccggcat aactgctacc
    45601 acaaccatta aagcctttcc aaatttttgc caaaactcaa aactaaatat ctttttcatt
    45661 ccgtttagct cctttacgca atcggtttcg ttaaacgtat tatagcgtgc gttttctttt
    45721 ttgtaaatgc tttatttttt atttttctgt ctataccatt ctataaaacc tataatacgt
    45781 gtgttccctt ctataatttt ctcctaaaaa tttatttttt taagcttttt tctatgaaat
    45841 attttctttt gtctattgac ttgtgcaatc ggttgcgttt attatgaacg tataaaacag
    45901 ctcattagga ggagaaaaat gaaacagatc aaacgactat ttgctattga tccatggaaa
    45961 atcagcacga cacaattaga taaaaaaaat cttcgactgc aagaatcttt aacaagtatt
    46021 ggaaacggtt atatggggat gcgcgggaac tttgaagaaa cttactcagg ggaccaccat
    46081 caaggtactt atttggcggg tgtctggtat cccgataaga cacgtgtcgg ctggtggaaa
    46141 aatggttatc cagaatactt tggaaaagtg atcaacgcct tgaactttat cgcgatagat
    46201 atttcgatca acgatcagct gatcgacttg gcttctttgg aaccagaaga cttttactgg
    46261 gaattagata tgaaaaatgg tgtattatct cgtacattca caatcactac ttctactaat
    46321 aaagtacgca tttccttcga acgttttctg agcattgtca aaaaagaatc agcttatatc
    46381 tatttaacta tagaaatgct tgaaggtagc ggaacagtca aactcgtatc aaaattggat
    46441 ggaaatgttc aaaacgaaga cagcaactat gatgaacatt tttggaatga gattgatcgt
    46501 ggaactatag aagaaatcgg ctatttgacg actaaaacta ttccaaacaa ctttgggatt
    46561 gaacaattta ctgttactgc agcaatgcgc catttcgtta atggaaaatc agtcatccct
    46621 gactatcacg aagatacttt agcattacat ggttgcttgt ctgcagaatt atctcaaggt
    46681 gagacatggt cagtggaaaa agaaattatc gttcttacta gtagagatat tccagaagcg
    46741 cagcaagcag aaaaagctgt ctcagaatta acgaaccaga caaacaatta tatccaagca
    46801 aaagaagagc aaacagcggc ttgggcagaa cgatggaagc ttgccgatgt ggtcatcgaa
    46861 ggagacgaag aagcacaaca aggtattcga ttcaatttgt tccaactatt ctctacttac
    46921 tacggcgaag atgaacgatt gaatatcggc cccaaaggat tcacaggaga aaaatacggc
    46981 ggagccactt actgggatac agaagcgtat gctgtccctc tttatcttgc tttagccaaa
    47041 cctgaagtaa ctaagaatct gctgaaatat cggcataatc aactgccgca agcaatccac
    47101 aacgctcagc aacaaggatt aaaaggcgcg ctttatccta tggtcacctt cactggtgtc
    47161 gaatgccata acgagtggga aatcacattc gaggagattc accgaaatgg cgcgattgcc
    47221 tatgcaatct acaattatac gaattacacc ggcgatacgg aatatttgaa aaatgaagga
    47281 ctggaagttt tagcagaaat cgcacgcttc tgggcagacc gtgtccattt ttcaaaacga
    47341 gcaaataaat acatgatcca cggtgtgacc ggcccaaacg aatatgagaa caatatcaat
    47401 aacaattggt atacaaatac gatcgcagct tgggtcttgc gttacacgcg agaaaattat
    47461 ttaacttatc aaaaagaagc tgctatcact atcagcgaag aagaactagc caaatggaaa
    47521 gatatcgcag agaacatgta cttcccagtt gacgaagaat taggaatctt tgtccagcat
    47581 gacacattct tagataaaga tctcatgcct gtatccgata ttcccacaag cgatcttcct
    47641 ctgaatcagc attggtcttg ggacaaaatc cttagatctt gcttcatcaa gcaggcagat
    47701 gtactacagg ggatttattt cttcaatgat acttttacaa aagaagaaaa gcaaaaaaac
    47761 tttgatttct atgaaccaat gactgtccat gagtcttccc tttcgccaag tatccattcg
    47821 attttagcag cagaattagg aatggaaagc aaagctgtag aaatgtacca gcgaacagct
    47881 cgcttggatt tggataatta caataacgac acagaagacg gcttgcacat cacttccatg
    47941 actggtagct ggttgtcgat tgtccaagga tttgctcaaa tgaaaactgc ccatgatcag
    48001 ttgagattcg caccatttct gccaaaaact tggaaagctt attctttcca tatcaattat
    48061 cgcggacgtt tattgttcat ctcagtcaca gatgaacaag ttactttcga gttattagaa
    48121 ggttctcctc tagaaattta cgtctatgaa cagtcttatt ctttagcgga tcgtctagaa
    48181 ataacgctaa gaaaggagga agcaaatgtt taaaggggta ttgtttgatt tagacggtgt
    48241 cattaccgat actgctgaat tccattatcg tgcttggcga aagcttggtc aggaaatcgg
    48301 cattacgatc gaccacacat tcaacgaaca gctaaaaggc gtcagccgag aagactcact
    48361 ctctttgctt ttagcttatg gcggaaaaga acactctttt tcaaaggaag aatttgcaga
    48421 attagcgaaa agaaaaaacg attattattt agagatgatc cagacgatcg aaccaaaaga
    48481 tgtcttcccc ggggtcgtgc ctttactgga ttctttaaaa gaagaaaaaa tcaagattgc
    48541 tcttgcttct gcaagtaaaa acgggccatt cctacttgaa aaaatgggtc ttacacctta
    48601 ctttgatgct attgccgacc cggcagaagc aacaaacgga aaacctgcgc cagatatctt
    48661 cctccttgca gcaaaagctg tcggactaaa agcagaagaa tgtatcggga tcgaagacgc
    48721 gcaagccggt atccaagcca tcctctcaag tggcgctcaa ccaattggcg tgggacgtgc
    48781 tgaagacctt ggagaggaga tcccgcttgt ccctgatacg acttttctta cgatcgatta
    48841 cttgaaagaa aagtggcatg accatggata atccagagat ctccgtcgta ccagttccag
    48901 agactgaaat aaagaaaatc acattgaaga acgactactt agaagtcgtt cttctcaatt
    48961 acggtgcccg cctgcaccaa atcttcgcac cagacaaaga aggaaaatca gaaaatgttt
    49021 tattgtctta cgatcgtttc gaagatgttc taacagacaa aagctttttt ggagcaactg
    49081 tcggacctgt agccggaagg atccgggatg cttcttgggg tgagcataga ttggagaaaa
    49141 actgcggatc acaccatatc cacggtggca cgaaaggctg gtctttccaa ttttgggaag
    49201 tagaagtttt caaaactcca tcatcaatag gtgtggtctt ttacttaaaa gatgaatttt
    49261 ctacctatcc aggaccaatc actgctacga tcacttaccg tttaacaaaa aacgaattag
    49321 agatgacaac ctctgctagt agccttgttg aaaccatctg caatccgacc aaccatgcct
    49381 acttcaacct ttcaggaaat ggaacaagag acatctatga acatcagtta acggtctttt
    49441 tagatggtgt gcttgaatta gatcaagaaa aactgcctac tggaaattgg acaaaaaaag
    49501 aaaaactgcc aatcgacttt agaaaatccc caacactgca agagatcctt gcatgttatc
    49561 cagatggact agatgatgta tttcttttgc atcatcctag gctttccaaa acttccttac
    49621 aattattcga gaagcattct ggtcgccaaa tgactatagc aacttctaat aaaagtatgg
    49681 tattattttc tacgactgga ttcgaagctg atttctctgt caatggaaaa cagatgcatt
    49741 caaactacgg attagcaatc gaaccgcaag aaatccctga tatcgttcat ttccccaaat
    49801 ttggatcgat caacctccat cctggtcaag aacggatcag tcagacgatt tatcggttct
    49861 ctgcacaata acaagagaaa ggtgttttta tcatggctat taccgtaaaa gatgtggcga
    49921 aaaaagccgg tgttgccacc tcgaccgtct ctcgtgtcat caacgatcat cctagtattt
    49981 ccgagacaac aaagaagaaa gtccgaaaaa ttatggatga actaggttat gttcctaaca
    50041 tgactgcccg caatctagga aaacgcattt ccagtgccat cggcgtaatc ttaccccctc
    50101 ttgattcaaa agagcggatc ggtaacccat tttatcttga gatcatggaa gcaatcaacg
    50161 aagaagcgcg gaattatgat atgactacag caatcgctac tgctaaaaat tttgaattgc
    50221 tgttagagaa cgtccagcgt atgcatttac aaaaacaagt agatggtttt attctagtgt
    50281 attcagatag tcaagatact gtcattgatt atttatacca aaaccacatc cctttcactt
    50341 tgatcggaca gccttatcat catgagaacg aaatcgttta tgtagataat gataaccagc
    50401 ttctaggtaa acaagcaaca gaatttttga tccagaatgg acataagaat atcctgtttg
    50461 tgacaaacac gactcatgaa agtttgtatt tcgaacgata tttcggttat caaaaagcaa
    50521 tgatgatggc tggtcttcct gcgttatcca gtgtagtact agagcatccg gaggattatc
    50581 tgtcctttga gaaaacgttg aaagaaaacg aagccacagc attggtggtc attgacgata
    50641 tcttcgccct tcgcacgatg cagttggcgc aaatgtatgg ctatgtcatt ccagagacac
    50701 tctccgtcat tagcttcaac aactcgatct tctcaacatt aacacaccct taccttacaa
    50761 gtatcgatat cgatatctcc gaattaggac gtgtcgctat gcaaaagctg cgcgaattga
    50821 tccatgaaga attgagcagt ggcgtacgtt tggtcatccc tcatcgcttg atcaaaagag
    50881 agacagtcgt tcctttagaa aacccatact aaatagctga ctcactaaat acgctgacca
    50941 tttatcctat ggtcagcttt ttatgtaaaa gaagatacta cttctaacac attgtttatc
    51001 tgtaaaaaag agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta
    51061 taaacggcgg aagcagaagc aactccttcg gaaataagct gaaattcaca aaaatttaaa
    51121 aagcaatttt cttgagtttc ttcttatttc ttggagttaa acgcttctgt cccagcctct
    51181 tttttccttc cttcaatttt ttacacgtcg acgattcaat aaaaaaccgc ctaatattgt
    51241 aaagacaggt gcaagaatcg agaaaaaggc gaatggcaag taatgcaacg cgctaacttt
    51301 catcgttccc ataatgaatg tgccgctgac tccccacggt acgagagaat tgacggtcgc
    51361 acctgaatcc gctagtgttc gtgtcaaata ggtttttgat aagccaacgt catcaaaaga
    51421 acgcttgaat gtttctcctg gaagaatgat ggacagatac tgttctccaa cgatcagatt
    51481 gattccgata cagctcaagg cagtaaaccc gactaaacga gaaggtcggt ccatcttttc
    51541 ttttaattct tgaacgatgg tatcaacgat cagatatttg atcagtaacc cacctaatgc
    51601 caatgcaagt aaaatcaaac tagcagaacc taacatactc atgatcccgc ctcgagaaag
    51661 cagttcatct agctttggat caggtgtatt tgcgacataa ccattcatca agatgtctgc
    51721 tgcttttccg accgtcaaac ttttctcatg gataaaagaa agaacgagag cagctgtcga
    51781 tcccgctagt aatgtaggga tagccggaat ttttttgatt gcccccccaa ataaaataac
    51841 tagtggaatc aatgtccaag gagagatcca aaagctcgct ttcatgtttt ggatcatttc
    51901 attgatacta gctagatcgg ctgaatgact gttatgtcct aatatcgtat aaaaaatcaa
    51961 cgaaaggacg aatgctggga aatccgtata acgcatacca gcgatatgtt cgaatagatt
    52021 aactcctcca atacccgctg ccaagttggt cgtatctgat aaaggcgagc agttattccc
    52081 taaaaaggca ccggaaacga ctgcgcctgt agtcattgca tcattgaatc caagtaaatg
    52141 accgatcccc aaaaaagcaa ttccaatcgt cgaaatcgtt gtaaatgaac tgccgacagt
    52201 cactccgaca attgcacaaa caagaaatgc tgtcggtaaa aagaaacgca cagaaacaag
    52261 tttaaatcca taaaccataa tcgtagggat ggttccagat aaaatccatg aagcaactaa
    52321 gacaccaatc aaaagaaaaa tcaggattgg aatgatccct ggagtgattc ctttgatgat
    52381 cccgtcatgg atctcatccc atgtgaactt tttaaatctg ccataaaaca acaaacacat
    52441 gaaagctgca aggatcggga tatgtggcga gagctggaat ccgatgatca gtactcctag
    52501 gagtgccaac aacacgatca atacagcgat actttctttt aaagaaatat ttttattcat
    52561 cgtcgatctc ctatttcaaa tgaaaaggcc ctgccatgaa aaaactattc acttggcagg
    52621 acctcctact aaatcagcga caaaacgtcg gctgaaagac ttattttttt atttttcttc
    52681 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52741 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52801 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52861 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52921 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    52981 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53041 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53101 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53161 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53221 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53281 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53341 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53401 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53461 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53521 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53581 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53641 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53701 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53761 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53821 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53881 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    53941 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54001 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54061 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54121 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54181 gggattcttt gagtttattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54241 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54301 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54361 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54421 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54481 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54541 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54601 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54661 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54721 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54781 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54841 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54901 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    54961 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55021 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55081 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55141 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55201 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55261 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55321 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55381 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55441 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55501 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55561 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55621 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55681 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55741 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55801 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55861 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55921 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    55981 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56041 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56101 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56161 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56221 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56281 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56341 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56401 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56461 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56521 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56581 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56641 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56701 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56761 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56821 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56881 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    56941 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57001 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57061 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57121 cttctgcttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57181 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57241 cgatagattg caggatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57301 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57361 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57421 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57481 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57541 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57601 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57661 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57721 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57781 tttatgaaag caggaacctg ctagcatatg taattaatta tttcttcagg ataggatagg
    57841 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57901 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    57961 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58021 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58081 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58141 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58201 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58261 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58321 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58381 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58441 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58501 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58561 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58621 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58681 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58741 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58801 tgattttgtt ttagttaatt tgaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58861 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58921 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    58981 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59041 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59101 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59161 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59221 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59281 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59341 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59401 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59461 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59521 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59581 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59641 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59701 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59761 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59821 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59881 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    59941 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60001 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60061 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60121 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60181 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60241 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60301 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60361 tctgaggttt attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg
    60421 aattcat
//
LOCUS       DAYYME010000003        43568 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000003 DAYYME010000000
VERSION     DAYYME010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.003"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..818
                     /locus_tag="ACHBFW_000120"
     CDS             138..818
                     /locus_tag="ACHBFW_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM9316470.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            908..1942
                     /locus_tag="ACHBFW_000121"
     CDS             908..1942
                     /locus_tag="ACHBFW_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM9316471.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1956..2291
                     /locus_tag="ACHBFW_000122"
     CDS             1956..2291
                     /locus_tag="ACHBFW_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316472.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2275..4755
                     /locus_tag="ACHBFW_000123"
     CDS             2275..4755
                     /locus_tag="ACHBFW_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316473.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4811..6370
                     /locus_tag="ACHBFW_000124"
     CDS             4811..6370
                     /locus_tag="ACHBFW_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9316474.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6367..7731
                     /locus_tag="ACHBFW_000125"
     CDS             6367..7731
                     /locus_tag="ACHBFW_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM9316475.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7902..8972
                     /locus_tag="ACHBFW_000126"
     CDS             7902..8972
                     /locus_tag="ACHBFW_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9316476.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            8987..9655
                     /locus_tag="ACHBFW_000127"
     CDS             8987..9655
                     /locus_tag="ACHBFW_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9316477.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9675..10253
                     /locus_tag="ACHBFW_000128"
     CDS             9675..10253
                     /locus_tag="ACHBFW_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9316478.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10654..11565
                     /locus_tag="ACHBFW_000129"
     CDS             10654..11565
                     /locus_tag="ACHBFW_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM9316479.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11943..12860)
                     /gene="rnhC"
                     /locus_tag="ACHBFW_000130"
     CDS             complement(11943..12860)
                     /gene="rnhC"
                     /locus_tag="ACHBFW_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFM9316480.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13062..13499
                     /gene="zapA"
                     /locus_tag="ACHBFW_000131"
     CDS             13062..13499
                     /gene="zapA"
                     /locus_tag="ACHBFW_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFM9316481.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13557..14105
                     /locus_tag="ACHBFW_000132"
     CDS             13557..14105
                     /locus_tag="ACHBFW_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFM9316482.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14220..16580
                     /locus_tag="ACHBFW_000133"
     CDS             14220..16580
                     /locus_tag="ACHBFW_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM9316483.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16667..16981
                     /gene="trxA"
                     /locus_tag="ACHBFW_000134"
     CDS             16667..16981
                     /gene="trxA"
                     /locus_tag="ACHBFW_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFM9316484.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17163..18941
                     /gene="uvrC"
                     /locus_tag="ACHBFW_000135"
     CDS             17163..18941
                     /gene="uvrC"
                     /locus_tag="ACHBFW_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFM9316485.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19086..19985
                     /locus_tag="ACHBFW_000136"
     CDS             19086..19985
                     /locus_tag="ACHBFW_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9316486.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            19978..21240
                     /locus_tag="ACHBFW_000137"
     CDS             19978..21240
                     /locus_tag="ACHBFW_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9316487.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21401..23299
                     /locus_tag="ACHBFW_000138"
     CDS             21401..23299
                     /locus_tag="ACHBFW_000138"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFM9316488.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23629..24348)
                     /locus_tag="ACHBFW_000139"
     CDS             complement(23629..24348)
                     /locus_tag="ACHBFW_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFM9316489.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    24538..24585
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            24685..25017
                     /locus_tag="ACHBFW_000140"
     CDS             24685..25017
                     /locus_tag="ACHBFW_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316490.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25004..27007
                     /locus_tag="ACHBFW_000141"
     CDS             25004..27007
                     /locus_tag="ACHBFW_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFM9316491.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27026..27496
                     /locus_tag="ACHBFW_000142"
     CDS             27026..27496
                     /locus_tag="ACHBFW_000142"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFM9316492.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27517..28101
                     /locus_tag="ACHBFW_000143"
     CDS             27517..28101
                     /locus_tag="ACHBFW_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFM9316493.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28116..29102
                     /locus_tag="ACHBFW_000144"
     CDS             28116..29102
                     /locus_tag="ACHBFW_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFM9316494.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29092..29403
                     /locus_tag="ACHBFW_000145"
     CDS             29092..29403
                     /locus_tag="ACHBFW_000145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFM9316495.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29425..31206
                     /locus_tag="ACHBFW_000146"
     CDS             29425..31206
                     /locus_tag="ACHBFW_000146"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFM9316496.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31199..32575
                     /locus_tag="ACHBFW_000147"
     CDS             31199..32575
                     /locus_tag="ACHBFW_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFM9316497.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32582..33217
                     /locus_tag="ACHBFW_000148"
     CDS             32582..33217
                     /locus_tag="ACHBFW_000148"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFM9316498.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33247..34602
                     /locus_tag="ACHBFW_000149"
     CDS             33247..34602
                     /locus_tag="ACHBFW_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM9316499.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34794..36188)
                     /locus_tag="ACHBFW_000150"
     CDS             complement(34794..36188)
                     /locus_tag="ACHBFW_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM9316500.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36280..37251
                     /locus_tag="ACHBFW_000151"
     CDS             36280..37251
                     /locus_tag="ACHBFW_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM9316501.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37248..38030
                     /locus_tag="ACHBFW_000152"
     CDS             37248..38030
                     /locus_tag="ACHBFW_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFM9316502.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(38199..38570)
                     /locus_tag="ACHBFW_000153"
     CDS             complement(38199..38570)
                     /locus_tag="ACHBFW_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9316503.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38704..39147
                     /locus_tag="ACHBFW_000154"
     CDS             38704..39147
                     /locus_tag="ACHBFW_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM9316504.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39190..39999)
                     /gene="yidA"
                     /locus_tag="ACHBFW_000155"
     CDS             complement(39190..39999)
                     /gene="yidA"
                     /locus_tag="ACHBFW_000155"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFM9316505.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(40018..41385)
                     /locus_tag="ACHBFW_000156"
     CDS             complement(40018..41385)
                     /locus_tag="ACHBFW_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM9316506.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41369..42199)
                     /locus_tag="ACHBFW_000157"
     CDS             complement(41369..42199)
                     /locus_tag="ACHBFW_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFM9316507.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42362..42799
                     /locus_tag="ACHBFW_000158"
     CDS             42362..42799
                     /locus_tag="ACHBFW_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFM9316508.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42875..43495
                     /gene="rpoE"
                     /locus_tag="ACHBFW_000159"
     CDS             42875..43495
                     /gene="rpoE"
                     /locus_tag="ACHBFW_000159"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFM9316509.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        14265 a         7488 c         9247 g        12568 t
ORIGIN      
        1 catgtaaacg attttattgt ttttttctct tttatacaaa aagggtcata atttcaagta
       61 agcggatgaa ttcatgagga ttttttgcat actgtctcta ctatacttag gtcactaaga
      121 aaaaggagtg atttattatg aaaaagaaca tgattgggac attattactt tcaacacttc
      181 ttctaggcgg cttggcaaca ccagcttttg ctgagggtca agcgacttca aaaggagata
      241 ttacctttac tgaaccaaca aataccgtgg agcctcttaa ccctactgat ccttcaaaac
      301 cagtagagcc agcagatccg gaaaatccag caactggcca gacaggtcct ttaacactgg
      361 atgtagttcc cgaattgcca tttggtacac acgagattga aagcggaaca aaaacgtatc
      421 aagtagacgc ttcaaaaaac gatacacctt atctccaagt atcagatcgt cgaggtgtag
      481 gtgcagatgg ccaagcacaa gggtggaacg tgacagtttc ggtttcagat tttgtaaacg
      541 gttcacaagt tttgcaaggc gctgaattag attttggaac atccactgtt aaaagtacgt
      601 cagataacga atccactgga ccaactagtc aaactgtaac tgggttgagt aaagcaagtg
      661 cagcgacacc aatatttaca gcagcaaaag atcaaggtct gggaacatgg ctttctgttt
      721 atgatccagc aaatatcaca ctgaaagtgc cgaaagcagc agctggaaca ttcacggctg
      781 atttgacttg gaacctcgtg gctggcccag tagcatagag tatgaaataa attcaacaaa
      841 ctgcggtgaa aatactatag gtgaaggatt ttttaccgca gtttttgttg aaaggatgtg
      901 gtggacaatg aatcaaagta aacgactgtt tgtttcgttt ttttctattt taagtctatt
      961 tttcattttt ccgtcaatca gccaagcaaa ggattcagca ggagactttg gaatcaaacc
     1021 ggtatttcca gagaaccaaa tagataaagc aatcggttat tttgatttat tagtggcacc
     1081 ggaacaaaat cagacactag aagtaattat cagcaattct tcagatgaag aacgaacatt
     1141 tgaggtctct gtcaatccgg cggtaacgag tgatggtgga acgatcgatt atagtcaaaa
     1201 aaacccaacg ttagatgaga cattgccgtt tgatgtccgt gacgttttgc tcattgcgaa
     1261 aaaggaaatc aatgtatcag cacatgcaga aacaactgta cctatagaag tgaaaatccc
     1321 agcaaaaagc tttaagggac gtgtattagc gggtattcat gtttcaccta aagaagaagc
     1381 tgaaacagaa aatgctacag aaggagcaca aatcaaaaat cgtattgctt ataatctcgc
     1441 agtcgtttta caagaaagtc aagagacgat agaaccggat ctgaaacttt tatcaggaga
     1501 tttggacgag gtcaacgcaa agccaactgt tcagcttcgt ttccaaaatc ctcagcctag
     1561 gattatcagc aatttgatat ttacgagcaa gatcttttat gaaaatcagc tttatattga
     1621 aaatacgtct aatgcatttt tagttgcacc aaattcgaat tttcacttga atcttgattt
     1681 agctggtgat aaagcaaaag ccggcgatta ccgcgcagaa atcatagcaa aatcaggcga
     1741 cagtaatgaa tggcgattta cacaaaactt tacgatcaaa aaagaaaaag cacaaaaagt
     1801 aaatgaaaat tccgtttttg cagtacaaga acaatctttc ccatggcttt atgcaggatt
     1861 ggttgctgca gttctagtgt tatggttgat tttgctgttt ttgtgggtcc acaagaaaaa
     1921 gaaaaaagga gaaacaacat gatgaggtga tttcgttgaa aacatcgatc ttattgggag
     1981 ggattattgc aagtttgctg ttcttcagct ggcaagagcc agtccaagca gataccaaaa
     2041 gttcaggtac gattacattt tatgatctaa gtccaattcc gcatccaagt ggttcagtca
     2101 atggcgcatc aaaacagcag acacagctac cgatttataa agaatctaat caacatggcg
     2161 tattcccgga gacgaatgaa caaaagatcc gttttcttta tttgatcatt ggtatagaat
     2221 tatggcttct tgcgtggttg ttgttatgga gaagaaagga aaaggaggat gaacatgttg
     2281 ctacaaaata aattttcatt ttttcgctcc ttctttttta cttcgctttt tgttttaagt
     2341 atatttatct tttcaccttc ggcagaagct gttggagcaa cgcctcctgc catttcttat
     2401 gatccataca cgtatgaaaa tatcgtacct ccaaatggtg gacgtagtgc agtcaatggc
     2461 gggaagcttg tccaactaac tggggctaac caattgccta aattgactgc tacttatttg
     2521 aacgatggtt caaatgatcc ttggaaaggt ggcacggtcc gttttcattg ggaaagccat
     2581 gtgaaactaa caaatgtact tgatttggtg aatatcactg cacaaggatt tgcatttgtg
     2641 gcgatcatca atttgccgga acatgtatca agcagtgatg tattaaaagc aattgactgg
     2701 aacaacgcct acttgtatat tgccggtgcc cccatcaagg ttactagcgg aagtttatta
     2761 tcagcaggaa gccatacctt gcgttttggg atggggtcgt gggatacaaa tactctattg
     2821 acagatatta ttgctattat tacgcgaggc gaattcaacg tggacgatat cccgcttcaa
     2881 tttgatgtta cagctaatgt cgcgaagatg acagaaaacg gagataaata tgatacgagt
     2941 ccaaacaaaa ttttgaccaa gggtaaatta caaccttcta tgtctaaaaa ggccgttttt
     3001 tctgttgatt tttatgattc gaataatatt atccaagatc gtatgttcaa ttcgatttta
     3061 gctccagctg caggtagatt ttatcctaaa ttaactggcg gaaacttgaa tccaggagca
     3121 tatgcatttt ctgcaagtgc agagataaat acgtggaata gttacatctc accaagtgat
     3181 tcaacaggaa aatataatac atttgctgat accacagaat acgtggatgg aaataaccag
     3241 aaccttccag gctctaccaa tagtcggaca ttgacgatgg ggctgactga aggacgtttt
     3301 gatcagttgc catttagtcg ctttgatcgc gtagtcaact attttacagg aaaaaacgtt
     3361 acacaaggag caatactgac tcatactcca gcaagcacaa gtggattgaa tcagacagcc
     3421 aatatcgtgt acagtgggaa agatgcgcaa ggcggattgc tttctcctgt tgctttaaag
     3481 gtattggagc agtacaagct agacggaacg gttttaccaa caaacttaac agccgatagt
     3541 cagtggggaa agctcctttc ttctgcatcg taccaagtca cggccaattg gtcagcaact
     3601 gaactaaaaa agggttcgat ccattacagg ctttattcga aaggaaccca aaaattagcg
     3661 acaggatgga cagatcaact tttccaaacg atccaatcaa ataacggggg tgtaaataca
     3721 gctacttcta cactacctgc attggcttca gggcaatatt attttgatta tcgcttggtg
     3781 gacgatgtac ttactaacat gtatccgtct tttgcgtaca aatggcagtc tgaacaagct
     3841 ggaatcacca ctcttcctca aataacagtg gctaattttc catctgtgag tgcagatagt
     3901 cagctaaaaa accttagcag acaacctgag acaggagatc ccctgtctgc tctatcagga
     3961 gatacgattc aagaaaatgt gacattcacg ttaacaaaat ctggggatgc gataaccgac
     4021 aaaaaaatca cgatctcact tcctgaaaat actacttata tcaatggttc cttaaaatta
     4081 aatggtacaa ctatcgcaga taccggcatt caacaaggag tttctgtccc agcagatcta
     4141 ttgagcaaaa taggagatac cattcatctt acttacaact atcaattaaa tactgtagat
     4201 acttctttac aatccgtcag catactgaca aaggcagctg tactttccag caatattaca
     4261 ttagcagatg gtgctaagct tcctaaccca gttgtccaaa catctgctaa aacaattctt
     4321 gtccccaaac aagaattaac attagttaat gtaccagatg attttacatt tggtaacgat
     4381 ctccccaaac cgttgaagac aagttattat gaatcaaaag gcgatttttc ttttgatgtg
     4441 cgggatactc ggcttccaag cacgtctcct tggcaactga caggtacatt gacttcttta
     4501 tttaagaatg atcaaggtca agaattaagc ggaacaaaat tatacttcaa tcattctgga
     4561 tcaaagcaat tgattcagca aggtcaaaac acgttgattt atgagagtga tggaacagct
     4621 aaaggagagg ttctcgttga ctttcccgat accgatggtt tactcttaga ggtaaatagc
     4681 agtacgaatg ctcaacctgg cgcaacgtat caaggcatgg tcacatggga actgacagct
     4741 gggccaacca gctaagtgat tagaatttgt atggatctgt gataactgtc acagatcctc
     4801 tttttcaagg atgagaagga aacttctgct gttaaaatca gaaatgcttg aaggagtgga
     4861 cttggaactt ttcagtttgc ttgaaaaaaa tgaacaacgg caaatcaaac ttttccaact
     4921 tttattagag gaaaaaggag aggtttccgt taataacgta cttacccgct tatcagttga
     4981 tcgggctacc ttgaaagaag atctgtttta tttacaagct aacttggaaa atatcaaaca
     5041 attggaaatt gttatccaag aagggaatat tttattaatc agacatggaa atatagcagc
     5101 agtcgatgtt tattatcatt gttacttgcg ctattctaca aaatatcaga tacttcttta
     5161 tctgttggaa aatggctcat ttgatcggca gaaacttgtc atggaactta atatcagttc
     5221 ggctactttg tctcgcagag tgaaggaatt aaatgattct ttaaatgagt ttcatctgaa
     5281 aataaaaagc gggcggctga ccggtccaga aagtcaaatt cgttattttt atttccaatt
     5341 attttggttt ggacgtccct atcttgaaaa caaaaaagaa tacagtgatt tatcaggaaa
     5401 cactttttta gatctgctgc aaaaagagtt tagttttgct attacagaag aaggacgatt
     5461 gaaatttgaa atatggcttt ccattatgaa gaagcgaatg cgctgtacga aaaaatccaa
     5521 accagattat agcaatcttc ctgttggatt gtttcaaacg acacaatttt tattggacct
     5581 ccatacattt ttgacccgtt acttctttca atatgccttt ccttggaatc aaaatgaaac
     5641 gttggttttt tatcttttca tgatcagtaa ctttacatta gatacacata actcgcatgt
     5701 ccaggagttt ttgataagcg aaaaatacaa agacgacgta gtagctcaat tgaatcgaac
     5761 gtttaaacaa tcgatagaag aatatttctc tttttatgtt tttccagaag agatttcgca
     5821 aaagattgcg ttgacagtga tgcaaaatca cgctcatttt gcctatttta gaggatggat
     5881 gaatattttt ggccaagaag gattaaaaca gcggttaact gaagtagaag ataaacaatt
     5941 cctggtttta tgtgaaagat tggtcagaca aacatttgct attgttgatt ttactgaagt
     6001 agacgaggaa aatgcccgct ttgaattatt tgctcgctat gcatctattt tacggacgct
     6061 gatcaagcag ttagaattac gtctgcaaat tgcttatgat cttccttatg aaaggctaat
     6121 ggcggatatc gtgaaagaaa tcatccagga acaattgctg aatacgatca aatatgattt
     6181 agtcgaatac gagaaagaaa agcagtatga tatcattttg actagccaat tgaaagagta
     6241 cccgtcacag aagtccgcta aagtttttgt attcactagc aacgaatata attatgattt
     6301 cccctatttg aaccaatttt taaaagaatg ttatttagaa aaattgaatt taaggatgaa
     6361 taaaacatga aaatcgtgat cattggtgct tcccatgaag gcatcacagc tgccctaaat
     6421 ctaagaaaac ttcaaccgaa agcagaagtt ttactgatcg atgaagatca taaagatgga
     6481 ctaggctatg tttcaaatgg aatcaacctt tatttaaaag gaaagatacg tagtttagct
     6541 gaagttgccc ataacatgcg gactcttcaa gaaagtgggg caaaactgat caccgaatgg
     6601 agagtaactg aacttgatcc agacaaacat cagctcatcc tcgcatccaa agaaggaaaa
     6661 aacgagacca taacctatga taaattaatc gtagcgacag gttcctctcc tgtcaccttg
     6721 tataaacaaa ttgaagctga aaatgtctac acctataaaa atctagtgca atctaagcaa
     6781 gtacttgccg ctttaaaaga agcaaaagaa gtagtgatct tcggtgctgg ttacattgga
     6841 ttggaattag cagatgcttt gagaaataaa ggatacatta ttcatctagt tgattatatg
     6901 ccgaatgttt tatcaagata tttcgataag gatatgatca acagttttca aaatcagctg
     6961 caaactaagc aaataaattt ttatccaaat gaattcttga tcgattggaa aaaatcagaa
     7021 gagaaagtag tatccgtaca acttctgtct caaacaataa aggcagatat ggtcattttt
     7081 tctgcccaaa ctcgtcctaa tacgacattg ttaaaagaaa aagtggcttt gtatgaggat
     7141 gagactgtga tagtcaatga atatctgcag acaagtgatc cggacatcta tgcgattgga
     7201 gatatcgtcc cagtcagttt tgacaaaaat aaacggcatc tctttttacc tttagttact
     7261 cgagctgtac atatggcaag agcagtagcg ctaactttga gtggtcagcc aaccgcttac
     7321 gacctcagac aaaagataac agcagcagtc attatagact actttttagg aactgtaggg
     7381 ctgacagaag atgaagcgcc attcttggaa caaaataccg gttcgtgttc aggagaattt
     7441 gatctatttc cacaatacga cgaagaaaac aaaacagtaa atgcaaagct gatctaccat
     7501 ccggatacat tagaaataat tggcggacag ctgataagcc aagaattctt attatcagat
     7561 ttgaacctgc ttgcggatat cgtgaaacac aaaactacga ttccacagct agcagttgag
     7621 gattttggat ttctgtctga atacacctct cgttttcact acctcaatga gttagctttt
     7681 aaagtactag ctaagagagc aaatagcaat agagtagata aaagactgtg agaaatatca
     7741 cggtctttta ttgtgtgggg atataataaa tattaatcaa tcgagaataa aaaattcata
     7801 taatgaaata gcggaagaat tttttgagca taatattcgc caaaaattta tcaagtgata
     7861 aaattgttgt ataaaatgat tatttcagga ggaggagtta aatgttttta gcattaaatg
     7921 aaattatgca ttcaaagtta cgttatgcgt tagttgcagg ggtgatgttt ttgatcgcct
     7981 acctggtatt ttttttgact gggttagctt atggcttagc tcaagataat cgaacagcag
     8041 tcgacaagtg ggaggcagat tcgattgtgc tttcgaaaga tgcaaattca aatctaggga
     8101 tgtccatgat tactaagaag atagcagaag aagtcgaagg tgggaaagtg gcgtatcttg
     8161 cccaaacacc tggagtcgtg actagtaaag acagtacaga agaaggaaaa atcaatgtta
     8221 gcttcttcgg tatcgataaa aatcaattca tcatgcccaa tcttgttgaa ggaaaagctt
     8281 ttgataacga tgatgaggca gtaggcgata ttagtttgaa agaagaatat ggtctagcca
     8341 taggagatac agttaagtta tctggtagtg acaagacgtt taaactaact ggctttacgg
     8401 atcatgccaa attcaatgtc tcaccagttc tctatacaac catcaatgct taccaagaaa
     8461 tccgcttcga aaaagaagat acaagtgaaa atgcacggat taacgcaata gttgtcagag
     8521 gtaaaatcag taatttacct gaagatttag aacagatcaa aatttctaaa ttcatcaatg
     8581 aattacctgg atataatgcg caagtattga cttttggatt tatgatcgga tttttgatcg
     8641 ttattgcggc tattgtgatc gggattttca tttatgtact gacgatgcaa aagattaata
     8701 tctttggtgt aatgaaagca caagggatca caggaggatt tattgcgcgt tctgtagtgg
     8761 cacaaacatt cattttatca tttgttggta ttcttcttgg tctattagga acagtgggaa
     8821 catcccttgt tctacctgat gcggttccat tccaaagcaa ttggctgttt tttggcgtca
     8881 ttagcttgtt gatgttagta gtagctgtgt taggcgcact attttctgtt cggacaatcg
     8941 taaaaatcga tccgttgaag gcaattggat aagaaggagg aagaacatga aagcaataga
     9001 atttaaagaa gtagaaaaaa gtttccaaga tggcgaccaa gtagttaaag cattgaaaga
     9061 aacaaccatg agtatcgata aaggggaatt tgttgcaatc atcggtcctt ctggttcagg
     9121 taaaagtact tttttgactt tggcaggcgg attgcagacg cctagcagtg gctctgtatt
     9181 tatcaatgat caagaattca gtaatcaaaa agaaagcgaa cgcgttaaac tacgttttaa
     9241 agaaattggc tttattttgc aagcttccaa tctggttcct tttctgacag taaaaaagca
     9301 gttattatta gtagataaga ttaaacatga acatcgtcaa aaagaagcaa acgaattatt
     9361 tgagcagctt ggcgtggata agttgatcaa caagtatcca gaagaactat ctggtggaga
     9421 acggcaaagg gtcgcaatcg ctcgtgcatt gtacaatgat ccgtccatta ttctagcaga
     9481 tgaaccaact gctagtttgg attcagaaaa ggcacgagaa gtagtcgata tcttggcaaa
     9541 agaatcaaaa gacaaaaata aagcaacgat catggttact cacgatacac ggctgattga
     9601 caaatgcgat agaatattta tgatggaaga tggtgtattg aaagaaaaag aataaccaaa
     9661 aagcaggtgg aaaaatggtc catcgataca acaaagatac acagcaaaaa attttgaaag
     9721 aagcgactga cttgtttatg tccaagggat atctcggcac ttcaacaaga gaaatcgcac
     9781 aaaaagttgg gatcacccag cccaatctct atcattattt cggtgataaa gaaaaactat
     9841 atacggcagt tttagaaaac cacttaaaag atgttggaaa agctttgaga gaaatcgttc
     9901 aaacaagcga aacgggattt caagtaacct tgacaaaaat ggcgcagttt ttgattgaga
     9961 cacatctggt cgatttgttt ctgatgcttc acgatcttga aagcaatctt tcaaaggaaa
    10021 caagagatca tcttttcttt ctatggaaga aaaattatcg tgaacctttt gaagaaatct
    10081 tttcagcaaa ccaatctgtg ctaagaaatg ggatttcaaa agaaatcgca gcaaggcatt
    10141 ttttcttagt gttagcacct tacatcactc ggtcgaaaag acctgtggaa gaaccgctaa
    10201 atgtggcgca attgatcgat ttatatttga atggtgtatt aggagaagcg taaagctgtt
    10261 atatagggag aaaatagtta ggtgaagaag caagatatga cgaagatcgt tatatcttgc
    10321 ttctttatat aggtagtttt tcataaatac aaatttgtta ttctctcatg attatcaggg
    10381 tatattttta tttattatgt cggtgttcca ggaagtgttt taatttaata acgaagaaat
    10441 taattgtttg tagaatgaag gaaactattt attgggtaga tgaatcacca cgaatagtca
    10501 aaaggagatt tcaggatatg ccaaaaatga aaaaaatcat tttagcagta atgttatttt
    10561 ctacggttat atcttttaca gccccttttt tgcttactta agctgatttt aagttttggc
    10621 ccttagtctg gatctacgag gtgagagtca agaatgaaaa aaataattgg agcttttgta
    10681 tttgcaatca ttatcattat tatctgtttt caaatagatg tttttggtca aaaaaagata
    10741 gttggacaaa cggtcagcag tacgaacagt acggttacaa agacaaagga atcatttgat
    10801 gatacagaac aagagacatt aatcaaaatc ggaaaaaatg accgtacact atatacagat
    10861 gactcgctaa ctaccccact tgcaactata aacggcggag agctggcgga gtttctttct
    10921 gaaacaaaaa atgcttatca gatcaagaca aatgatggct atactggata tttagccctc
    10981 gaagatggaa ccaaagtcac aaagaatata caaacaaaac cggaagagct atctgatgca
    11041 gtgattgtat tagatcctgg gcatggcgga aatgatactg gggcattaag caatgatgaa
    11101 caaacagaag aaaaagatat aacattaagt acagccataa aagtaaaaaa ggcgctagag
    11161 gatgctggag caacggttta tttgacccat actacagatg aattagtaca gcttggagat
    11221 atatgcgact acagtgaaga aaaaaaggca gatgtcttta tcagtttaca cgcagactct
    11281 acggaatatg cgaatgaagc aactggaatc acgacatatt attactatgg acaagaggaa
    11341 acattagcac agacaattgc tgactccttc actgatctgc ctctggattc aagaggaatc
    11401 tccactggga actatcaagt tcttcgagaa aatcttcaac cttctatttt aatcgagatg
    11461 ggttatatga ataatgatag tgatttaaag gaattagtaa cagacagtta tcagcagcag
    11521 attgctgcaa gtttgacaca agcattgacc acttattttc agtaataaat aagcccagcc
    11581 aaacaaaaat agctcctcat atttttcgtt tggccgggct tattatcgca gatgcaagct
    11641 tttaaacacc gtttaaggaa gaggtcgtga gcctgccgtt caactctgag tcacaaggag
    11701 caatttcaaa aaacagcttc tcatgtttga tgactattgt gacttgtgac cgaggagttg
    11761 gcatgagaac accgtttaag aaaaaggttg tgaaatcaac gttttgttct gagtattaga
    11821 gaaacaaacg gaaaaatagt tcctcatatt ttgccgtttg ttgagctaat accgaggatc
    11881 agatgattga acaccgttta ttcggaacaa gggactgtga catgactttt gtcacagttc
    11941 cattattttt ttagtagctt caaagctttt tctgtattgg caaaatgaag tttggcatat
    12001 ttttcaagca taggcatgcc gcctttctcc aatagttttg ccgctacttg gtcagatttc
    12061 gttccagctc ctgacggaag ggtgattcca gcttcagctg atgctttgtc taactcttcc
    12121 aaaaagctgg cacgactgat gatagaagca gcagcaacag ctaaatgata ttgttctcct
    12181 ttagtaacga agtacagttt ttcttgtaat tgattttttt ctaaacgcgc atatttttta
    12241 aaatttgctt ccggcgtaaa ctgatcgatc aaaatcgctt ctggtttttc tggctcgatt
    12301 ttttttagca gcaaatggat cgcttgattg tgcaaagcga ctttcatccg aacggcattg
    12361 tattttggtt ggatctcatt gtatttttct ggagataaga caagcagttt ataggggatg
    12421 gtttttttta agatgtcagc aagctggatg atttggctat cttttaactc ttttgaatct
    12481 ctaacaccaa gtttacgtaa ttctgcaagc tgatcacgtt tcacataagc agcacatact
    12541 gttagcggac cgaaatagct gccatttccg acctcatcac tacctaaaac ggataattga
    12601 ctgatatttt ttggcaaaga agaggctgtt tttgctggct tggcttgttt atcctgtgcc
    12661 tccccccatt tatcagcttc tattgctgca ttcgtacctt gaaacatcac ttttccggat
    12721 gtataagcgg taatggtcgt cccgtctttt tttgcagcaa agctagtata aggaatctgt
    12781 ttatttaaca aataagagcg atagtatgct ttcatttgac taagttggtc gtttgttgct
    12841 ttgataatga cgttgctcat aaaattctcc tcgtttttat acataaaact ataccgcaac
    12901 tcacgacttt tcgttttcta agagattgcg agacatagca tttttttaaa gtttatcata
    12961 taatagttaa gtatgtgaaa aatatttgtg gagaacggtg aaagaaccaa cttcatggta
    13021 cactaaataa aactttcatc aaataggagg aaatgattcg tatggcgcac gaaaaaacac
    13081 gctacaaagc ggtgattgcc aaccaaacat atactattat cggacgggaa accaagcatc
    13141 atatggatat cgtaacgaag ctgatcaatg aacagttagc agaattgaaa caattatcac
    13201 cacaaatgga taatgaacaa gcagcaatct tgatggctgt caatgcgctt tcggatcaat
    13261 tgaagaaaca agaaagaata ttggagctgg aagaagaaac agcagaatta aagaaaaaaa
    13321 tgatcaaatt tacagagctt gaaaatcgtg tgaaacgcat tgaagcaatc gagaacgaag
    13381 ctcgggaagt attgaaagaa aatggtcaag cagatcatac cattaaaaat catgtggaag
    13441 cacaacagat tttaaacgag aaacgaaaag gacaaattaa acaaaaatct tctaattaag
    13501 atccttttat caaaataaga aaaatttaaa aaagttagaa gagaaaggaa cgtagcatgc
    13561 tgagtttgct gattttattt ttattgctaa ttgcgttttt ttctggagca cgtcgcgggt
    13621 ttgctttgca aatcatattc gcgattggat atgtattgtc atttattgct gcccagcatt
    13681 tttacaaacc attagcaagt catttggaat tatacattcc atacccagct gtcaccccaa
    13741 catcaaagtt agcatttttt gatcaggtat ttgcctttca cctagatgaa gctttttatg
    13801 caggaactgc ttttctgatt attttgctaa ttggctggct gcttacccga tttgtggggg
    13861 tttttgtcca tggattgacg tatgtgccga tcttgagaca ggtagactgg attgcaggag
    13921 gcatcctgtc actaatcatg gcttacgtta ctatattttt gattttgcag ttacttgcct
    13981 ttgttccatt agatgtggtc caaaatcaat tcggcgagca tagtttcgct cgttttatcg
    14041 ttgagagaac gccatttctt accaacaaga tgtatgagct ttgggtaacg aatattttga
    14101 attaaggaaa atagtgctgg aaaatatttc gttttttgta cagagtagtt tatttaagaa
    14161 ggctgcgtag cgtatgtccg gcacagctct tttttcatag tgaaagataa ggtgataaaa
    14221 tgaataaccg aatccttgaa acattggaat ttgaaaaagt caaacagatg gtcagacaat
    14281 ttgtagtgac tgcacaagga aaagaagaac tggcagaatt ggtaccagtg agtgagaaac
    14341 aaacgatcac aaactggctt caagaaactg aggatggatt gaaagtgcaa cgtctgcgag
    14401 gcgggatccc tatacctaaa attgaaaata tccgtcccca tatgaaacgg attgagatcg
    14461 gagcagactt gaatggagtg gagcttgcac aagtttccag agtattgtct acgacaagcg
    14521 aattgaatcg tttcattgat gatctttctg acagtgaaat cgaatttgca cgtttgtata
    14581 tgtgggcaga tcagttagtc acgatccctg ttttgagccg ccgtttaaag gaagcaatcg
    14641 atgaagacgg acgcgtgact gatgacgctt ctcctgagtt aaagagcata agacagaaca
    14701 ttcggcgaag tgaacaagct gttcgtgaac agttagatgg gatcgtccgt gggaaaaatg
    14761 caaagtattt aagtgatgcg atcatcacca tgcgaaatga ccgctacgtt atcccagtca
    14821 aacaagaata ccgcggtgta tttggcggtg tagtccacga tcaaagtgct tcaggtcaga
    14881 ctttatttat tgaaccaaaa caagtggtgg atttgaataa tcgtttgcgt caataccaaa
    14941 tcgctgaacg aaatgagatt caacgtattt taagtgaact ttctgctgaa cttgttcccc
    15001 atcgtcaaga aatcatccac aatacttatg tgattggtaa aatggatctg atgaatgcaa
    15061 aagcacgatt tggtaaagaa gtaaaagcca tcgtgccggg aatcagtgaa gacaatcatg
    15121 tcgtcttgaa acaagcaaga catccactga tcgatcagga aaaagtagtt tctaatgaca
    15181 tcacaatcgg aaaagactat caagccatcg tcattacagg acctaacaca ggtgggaaaa
    15241 cgatcacgtt gaagacttta ggtctgttac aactgatggg gcaagcagga ttaccgattc
    15301 ctgcaggtga agaaagtcaa atcggtattt ttgaggaagt atttgcggat ataggagacg
    15361 aacagtcgat tgagcaaagt ctatcgacct tttcttctca tatgacgaac actgtagata
    15421 ttttatcaaa agtcaatgaa aaaagtctgg tattatttga cgagctcggt gcaggaacag
    15481 atccgcaaga aggtgctgct ctagcaatcg caatcttaga tgatcttggt aaaaagtctg
    15541 cttatgtgat ggcaacaacg cattatcctg aattgaaagt ttatggatat aatcgggcaa
    15601 atacgattaa tgccagcatg gagtttgatg tggatacatt aagtccaacg tatcgtttgc
    15661 tgattggcgt tcctggacga agcaatgcgt ttgaaatctc tagccgatta ggtcttgata
    15721 cagaagttat tgatgaagcg aagcagttga tgaatgatga gagccaagat ttaaacgaaa
    15781 tgatcacaga tttagagaat cgccggaaaa tggcagagac cgaatatctg gaaatgcgtc
    15841 attttgtttc tgaggcacaa gaacttcacg atgatttgaa agaagcttac agctatttct
    15901 ttgaagaacg tgaaaaagaa atggaaaaag caaagaaaaa agcaaatgaa gtcgtgtcag
    15961 aggcggaaga aaaagcagaa aaaatcattg ctgatattcg taaaatgcaa cagcagattg
    16021 ggcaaggaaa tgtgaaagag catcagttga tcgatgcaaa gacccaatta gcaaatctac
    16081 atcaagaaga aactttgaag aaaaataaag tattgaagaa ggcgaaagaa caaaaaacgt
    16141 tgaaaccggg agacgaagtg ttggttacga cttacggcca aagagggacg cttttacgta
    16201 aaaacgggaa tcagtggcaa gtagaaatcg gtatattaaa aatgaatgtg tccgaagacg
    16261 agctgacgcc tgttgctcct caaaaagagc cgacacaacg agttatccat gctgtacgat
    16321 ctgaatctag tagtcacgtg ccgaatcaat tggaccttcg aggaaaacga tacgaagaag
    16381 cgctaagtga ggtagatcaa tatttggatt ctgccattct agctggttat cctcaagtga
    16441 ctattgtcca cggaaaagga acaggcgcac ttcgcaaggg aattactgat tatcttaaaa
    16501 accatcggag cgtcaaaagc tttgagtttg caccggcaaa ccaaggcgga aatggtgcga
    16561 caattgttaa atttaagtaa agcttactaa aggtaagcag atggttcgct atccaagtat
    16621 agaaatgcta tactagtcat agattttaaa aatggaggtc tttgatatgt cacaagtaat
    16681 tacagataag acatttaatg aagaaacaga taaaggactt gtattgattg atttttgggc
    16741 aacttggtgt ggtccatgtc gtatgcaagc accgattttg gatcagttgg aacaagaata
    16801 tgatgaagaa gaattcagaa ttgctaaaat ggatgtggat gaaaatccag aaactcctca
    16861 acaattcggt atcatgagca ttccaacatt aatgttgaaa aaagatggtc aagtagtaga
    16921 aaaagctgtt ggtgttcatt caaaagaaca actacgtcaa atgatcgatc aatatttata
    16981 aaaacttaca gcaaaaataa gctgagggtg ggactgaatc cagttccgtc ctcggttttt
    17041 ttgcggttga gttaaggtta ttcaaattaa tattttttga gtataattac gttgtcaggc
    17101 tggattgttt ttttgacaga catgctataa taactttaat tgaatatgaa ggggagtacc
    17161 agatgaacga acgaatcaaa aacaaattag ctttacttcc tgatcagccc ggctgttact
    17221 tgatgaaaga taaaaatgga acgatcattt atgtcggcaa agccaaaata ttaaaaaatc
    17281 gtgtccgctc atattttact ggtagccata atacgaaaac ggaacggctt gttagtgaaa
    17341 tcgaagactt tgaatacatc gttacagaat cgaatatcga agcattgctc ttagagatca
    17401 atctgataaa gaaaaatgat cctaaataca acattatgtt aaaggatgat aaaacctatc
    17461 ctttccttaa gataacgaat gaaaaatatc cccgtttggt gattactcgt aaggtactaa
    17521 aagacaaagc actgtatttt ggaccatatc ccgatgtcgg tgcggcgaat gagacaaaga
    17581 agatcctcga tcgattattt cctctgagaa aatgcaaacc tagtcagacg aaggagcctt
    17641 gtctttacta tcatttggga caatgcctgt gtccttatta tttcgacgta gatccgaaag
    17701 tctatacgaa aatcgttgat gaagtgaaaa actttttaaa cggaggacac gaaaaaatcg
    17761 agtcagagat ccatcagaaa atggaaaaag ctgctgaaaa catggagttt gaaaaagctg
    17821 cagaataccg agaccagatc cgtgccattg agacaatcat gactcgccaa aaaatgacaa
    17881 atactgattt actggatcga gatgtatttg gttatgcgat tgataaagga tggatgtgtg
    17941 tccaagtgtt tttcgtaaga caaggaaagc taattgaacg agatgtttca atgtttcctt
    18001 tctataacga agcagaagaa gatttcttga cttacctcgg tcagttttat caggaaaatg
    18061 agcatttcat tcctaaagaa gtcttgattc ctgatacgat cgacaagccc agtgtagaag
    18121 ctttgttagc tacgaaagtg ctgcaaccaa aacgaggaga aaagaaaaaa ttagttgaat
    18181 tggcgagtaa gaatgcctca gttgctttga atgaacggtt tgatctgatt gccagaaaac
    18241 aggaacggac gatcggagca gttgaacgtt taggaaacgc gatgaatatc ccgaccccaa
    18301 tacgtatcga gtcttttgat aactctaata tcatggggac gaatcctgtg gctgcgatgg
    18361 tcgtattcat tgatggaaaa ccggcaagaa aagaatatcg gaaatataaa ataaaaacag
    18421 tcaacggacc ggatgattat gcatcaatga gagaagtcat ctatcgcaga tattcgcgag
    18481 tgattcgcga agggttgcct ttacctgatt tgatcttgat cgatggagga aaaggacaag
    18541 tagatgtggc aaaagatgtc ttagaaaatc aattaggaat cgatattcct attgctggca
    18601 tggtaaaaaa cgataaacat aaaacaaatg agttgctatt cggtacagat ttggctgtag
    18661 taccgcttga gcgaaattca ccagagtttt tcttactcca gcgaatccaa gatgaagtcc
    18721 atcgattcgc aatcactttc catcggcagc tacgcagtaa aaacagtttt gcttcccgat
    18781 tagatgaaat tgaagggtta ggaccaaaac gaaaaaaaat gctgcttaaa gagttcaaat
    18841 cgttgaaaaa tatcacagca gccacgacag aagaattaca agaaatcgga ctgccgaaaa
    18901 atgtcgcaca aaacgtattt gataaattac atcaggaatg aggaaagggc actttacaaa
    18961 gaaagtaaag tgtcctatca ttttatgtac aacaaaaaga gcgtttacgt tttgttggta
    19021 aataataaat cgcttacgtc tggtgtctaa ggatatatgt gatttcttga agaaggagtg
    19081 aagaaatgtt agaagtcaag gatcttgtta aaacttttgg gaactacaca gctgtcgatc
    19141 atgtctcttt ccaaatcccc gatggaaaaa tcatggggct aattggccaa aacggggctg
    19201 ggaagacaac gacttttcgt ttgatcttgg attttttgac acaagaccaa ggagaagtgt
    19261 tatggaatgg ccatcatctt ggggagaaag attatgatgt catcggttat cttccggagg
    19321 aacggggact ttatccgaaa gtaacgattc aagaccaact gctttatttt gcagaactta
    19381 gagggaaatc aagaaaagag attgaaccaa aaatcgactt ttggatggaa aaatttcagg
    19441 taaaagggaa aaagacggat aaagtaaaat ctttgtctaa aggaaatcag caaaaagtcc
    19501 agctgatcgc cacgttaatc catgaaccta aattgatcat tttggatgag ccattcagcg
    19561 ggttagatcc ggtaaatgct gaattgctaa aagatggaat attggaattg aaaaaacatg
    19621 gttcatgtgt cattttttct agccacaata tggataatgt agaaaaaatc tgcgatcatt
    19681 tgatcatgtt gcgaaacgga aaaatggttt tgaatggaaa agtacatgag attcgtgaat
    19741 catttggccg tacgaagtta ttcttggaat ctggtttaag ccaacaggaa gtagaagaaa
    19801 tcgaaggcgt gaaaaaagtg atccttcatg aagacaatac gttagagatt accttagaag
    19861 atcctgaagc tgggaaagaa atctttactc gtgcgactca gttcggctac attcctatgt
    19921 ttaaccagca accgccaact ttggaagaga ttttcaaatt gaaagcaggt gaaatcgatg
    19981 cgtaagtttt ggattattac caaagatgtc tatttaaaaa atgttaagtc gatttctttc
    20041 ttgatcatga tattagtccc ttttatcatg ctgggtatca tttatgtagc aggaaacttt
    20101 gctcaacaaa atagtgatgt caataaaatc ggcgtattgg ctgataatga gcaaatcagt
    20161 cagcaaatga gccaagtcaa aacagatgat tttgagttca aagcagttga ttctgaagaa
    20221 aaagcacaaa aagagctttc ggaagaaaaa atcgatgcgt atttagtagt ctcaactgag
    20281 aatgataaga taaatgggat gctttatagc gaaaattcat taggacaaag tacacaacta
    20341 ctgatacaac aacaactgac tggcttgcaa tcgatgcttc gagcgagtca attaggcatt
    20401 tcaccagaag aagtagctag cttgagccaa caagcaagtt tgactcgaca aaaagtcaat
    20461 tttgattcaa aaggaaaaat gacagtcgga gaagataact cgacagtcca atacgtcgta
    20521 agttatgtag caacgattgt cttgttcatt attattttga cgtatgcgca gatcattgca
    20581 caagagatcg cttctgaaaa aggcacgcgg atcatggaag tgatcctgtc cagtacgact
    20641 gcacaaaagc atttttacgg aaaattaaca ggtgttttac ttgttgcatt aacgcaaatg
    20701 gcattatacg gtattatttt tggtatagga tacagtcaat tcaaagatat ggaaatggtc
    20761 aaaaatatat tagacggtat ctcattggat aacttgattg gaccattctt gtggtattct
    20821 cttctattca tgatctttgg cattttgatt ttttctgttt tagcagcttt atgtggctct
    20881 ttggtcaaca aagcagaaga tacagcaaaa gcaattttgc cagtgactta tctatctcta
    20941 ggcggataca tgcttggatt gatactagga gcttcagatc caaacaatat cgttattcgg
    21001 atcacttcgt atattccatt cttatcttca tatattatgc cgattcgctt agcaaatgag
    21061 acagtcggta tgggtggagc attgatttcc ttgttggtac ttgtagccgt aacattctta
    21121 ttgatgatct tgtctgcaaa catgtacaaa tcgaatgtac tcgtttatag cgaaggcgga
    21181 ttatggagtt cattgaaaca atcgatttca attatgagga atgaacgaaa aaaagcataa
    21241 aacaatccta aaatacagaa ggaagctgga catctattgt tcccagcttc tttttgtata
    21301 gacctatttt atttatttta ctatattgga tatatattca ttgaaaatag tgtataacaa
    21361 ctttataata ttgagtatac caatttttgg aggcgtttta atggaccgtt atttagattt
    21421 actttcagaa aaattcccct caacggagga tgtcatcacg gaaatcatca atctcgaagc
    21481 aattttgcag cttccaaaag gaacagagtt atttttgagt gatatacatg gcgaatttcc
    21541 tgcttttgat catattctgc gtatcggttc aggaaatctt aaagaaaaga tcaaagacct
    21601 tttttctgaa caaatgacgg aaacggatat cagccaattc actattttta cggcttatcc
    21661 agaatatgca ttaacgactg attggtacaa agaacaagat aaatcgcagc tgatccatca
    21721 tttgatcgat cttttacggt ttacgacagt caaatatacc cggtcaaaag ttcgaaaagg
    21781 attgccgaaa gaatatagct atatcattga ggaactcttg tatatcgatg accgtatcaa
    21841 tggaaaaaaa gactatgtaa aaaaaatcat tgaacaactt gttttgatga aggaagaaga
    21901 aaaattttta acgaaacttg cacagacgat ccaaaaatct gtcatcgatc atttgcatat
    21961 cgttggagat atttttgacc gaggaactca agcagccaaa gtgatggatc gaatcatgga
    22021 tttttcttct gtagatatcc aatggggaaa tcatgatatt ttgtggattg gtgcgtattg
    22081 cggctctgag gcatgtttac tgacactttt acggattgca gcacgctata attatttatt
    22141 tgaattagag aaagaatatg ggttgaattt acgtccattg ttttcttttg ctcatcaaac
    22201 ttatcagaag aatccagtat ttgcgcctaa aaacagtaaa aatctatccg aaagagagca
    22261 agaagaatta gaaaaaatcc atcaggcact ttctatcctt caattcaaat cagaacatca
    22321 gttattggat cgtcgacctg aatttaaaat ggacgatcgt aaattgctgg aaaaaatcga
    22381 ctatgaagca tcaacgattg atttagaagg tcagctctat gagttgaaag gaacttgttt
    22441 tcaaacgatc cagccagatg atccaaaaaa attcttggaa gaggaacaaa aagtgattga
    22501 tagtttgatg tattcattcc agcactcttt gagaatgcaa aaacatatga cgtttttgat
    22561 cgaaaaaggg ggaatgtatc tcacgaacaa tcagcatgtg ctgtttcatg gctgtatacc
    22621 tgtagacgag aagggacagc tcttagcatt tgaattagac caatcttatc gcggaaaaga
    22681 actgctgggc ttttttgaaa aacagataac tgaaagcgca aaagatctga ctgcaaaaga
    22741 agatttagca accgatttga tttggtatgc gtggagtggg ccattttcgc ctttattcgg
    22801 caagagtaaa atggcgactt ttgaacgtta ttttctcaca gagcctcata cgcacgtcga
    22861 gaaaagcaat ccttattatc acttgcgaga tgaagagtgg ttcagtgaaa aaattttagc
    22921 agaattcgac acaaaagaag aagggtcagc aatcattaat gggcacacgc cagtaaaagt
    22981 aaaaaaaggg gagtcaccga tcaaagcaaa tggaaaagta tttgtcattg acggaggttt
    23041 atccaaagct tatcaaaaaa cgacaggaat tgccggttat tcactattat gcaattctta
    23101 tggtttccaa atcgtgacac attatccgtt tttgcctatt gaacaacaat ttgcaaaaaa
    23161 atccgaccaa acaaatgtga aaaaagtaat cgaacagcaa ctaccacgca aactcaatcg
    23221 tgatacaaca aaaggtatgg aactgcagca gcagattacc cagctcaaaa ggctgttgga
    23281 ctatcgaaag gacgattgag aagaattcca gtgaacattg ttccatagac tggagactga
    23341 gacaaaagtc gtgtcgcagt ctctttttcc ggataaacgg tgttcttctg ccaactcctc
    23401 ggtcacaagt cacactattc aacaaacatg aaaagctgtt ttttgaaatt gctccttgtg
    23461 actcagagtt gaacggcagg ctcacgacct cttggtataa acggtgttca aaagccagtt
    23521 tctcggtatt aagccaaatt tatgcaaaaa tgtgaaaagc cattttcaca aaatttttct
    23581 taatactcga aactaaacag ctttttcaca accccctcag aaagtctatt aaaattcatt
    23641 cacaaagaaa tcgaataagc gctgttcaga tggatgtaca cggtgggtaa gttccggatg
    23701 ccattgaaca ccgaggattt tatgcatatc atctttgctt tgaaccgctt caacgagtcc
    23761 atcgttagaa aaagcgatgg cttttaataa aggtgaaaga tctttgactg cctgatgatg
    23821 gtaagaattc acaaaatatg gatcggggag aaggttgctt aacaaactgt ctgaaacaat
    23881 cttgacttcg tgagtcgtaa attgcggggc agtgggctgt tgttcatgtt ttactgacca
    23941 ctttggatat aatgaaagat cttgataaag tgtaccttcc aaaacgacat tcaatagttg
    24001 gagtccccgg cagactgcaa aaattggttt tctttgtttc aatgcttcca agatcagtgc
    24061 ctgttcaaat tggtctcgtt gaatatttgt ttccgttagc tttggatgag gatcttccat
    24121 gtaaagttgt ggactgacat cttgaccacc tgcaagaata agtttgtcga tttgtccaat
    24181 cagttgagga gctgaatctg gagacatgac tggcagaata agaggcaatc cttgagcatc
    24241 taaaactgca tccacgaatc cttgcggtgt atagctcact tgatttcctt ggaaagtatc
    24301 tgttgctcga atcagttgat ttccggcaat gccgatgatt ggtttcattt gtattccccc
    24361 taaagtagac ttattgtaac aaggatttaa aaaaaattaa actatgtaag acttgcccct
    24421 atacttatct gttcttgctg tgttactatc tgtaaagaga aatttcgtgt gaaattcatg
    24481 acgatagttg aaaatattgc ttaatttgtt ataataaaaa gtaagcagaa ataaaaaggt
    24541 tgggcgtaag cttcaaggat ctagttcttc aaggggtgag aaggacattt tattgaaggg
    24601 gcctgcctat actaagttat ctttagaggt acatactttc aatgaaaaaa gaaagcatgt
    24661 acctcttttt taaatcgaaa ggaagtgaag caagtgttag aagctttgga acaaatccga
    24721 aaagcagaag aagaaaatga aaagcgtgct caaacactgc ggcagcagct gaagatctat
    24781 gcaacagaaa aagagcagga attggaaacg atccaaacaa actatcgaca acaactaaaa
    24841 cagttggtgg aagaaaaaga acaatttgaa atacaagaat tagaaaaaga aaaaatgaaa
    24901 ttgaaagcag aagcagacaa ggtgcaagca actttgaaag atcaatatca aaagcatcag
    24961 agtcaagcga tcggtgcgat cattgaaagg gtgaaagaaa cttatggccg tcactaagat
    25021 ggaaaaagtg accctcattt cagataaaag aaatcaggag aggattttac aagcagtaca
    25081 aggaatccaa aacgttgaaa tccgggatct gtatcaaaat agaacaaaca accagtgggt
    25141 ggagaagtat tttccagata ccacgattat tgataaagaa gcaaaattaa gcgctttagg
    25201 taatcggttg aaagacatcc aagaagctat ccaattcata caacaccacg gcgatagccg
    25261 tcaaaagaaa ctccatttga aacgtcaaga actttcctta caagatttgg aagccaatta
    25321 ttccgaagaa aatttcataa ataaattgaa agaagtcctt gatttaaaag aaaagtggga
    25381 acaactaact gaagaacgtg atcgtttaga agaagaggaa gaatggcttc ttcattggca
    25441 acagctggat attgctccaa gtatgtatgc aagtaagacg accatttttg agatggggac
    25501 tgtgagtgca gcaaattttg aaagtttcaa ggaagaactg agtaagcttg atgacgttta
    25561 tatggaagaa atagattata cgccaaaaca agtttatctt tcgtatgttg ctttaaaaaa
    25621 atcagttgct gttatagaag aaattgctgg gaaatacggg ttcaataaag aaacgtatcc
    25681 ttatgaacaa aatcctaaag aaaaactgca agagaccaag cagcaattat ccattgttca
    25741 cgagcagcaa aagaagataa cgacagcgtt aggtcagtgc agtggctata tccaagaatt
    25801 tgaatgggta gaagaagtca cacttgccgt tgcagaacga gaaaaaataa aagaacggtt
    25861 tatccatgcg acttatctga tcgttttgca aggttgggta gatgcagaag aaaaacagaa
    25921 tttgctgcat gtgttaaacg aaaccgtatc agaagaagat atatacgtca gtttcgaaac
    25981 accaaactca gaagagattc agcaggaagt ccctactaaa ttgaaaaacc atccgcttgt
    26041 cgaacctttt gaattgctga ctgaaatgta cagtctgcca aaatatgaag aagtagatcc
    26101 aacaccttgg atgacaccat tttatcttgt cttcttcggg atgatggtcg cagatgttgg
    26161 ttatggtttg ctgatgctca ttgggacaat actagctcaa aaactacttg ttctaccacg
    26221 cgggatgaaa cgtttcgtaa aattctttga gattctctcg ataccatcga tcatctgggg
    26281 attgatctac agttcatttt tcggacagtc attaccaaaa gaactgtttg gcatccgctt
    26341 gcctttccca atcctatcta cgacggatga cgtcaatacg attttgattt tatccgtgat
    26401 tttcggtctg atacagattt tggttggatt atttgtggca gcaaaggaaa atatcaggag
    26461 aaaagattac ttgagtgctg tcagtgatgg atttgcctgg caagggatcc taattgggat
    26521 cgttattgcg ttggttggtg ccatgttgat aaaaaataca gcatttgttt accttggcgc
    26581 tgggctatcg atcattgctg ccttatgtat cttgatcgtt ccgattatcc aaacgccgtc
    26641 taaagtcaaa ggtgctgcca aaggtgcgta taatctttac ggactgaccg gctatatcgg
    26701 tgatttagtc agttatacac gtttgatggc attgggtatt tcaggcggaa gtattggagc
    26761 ggcattcaat atgctagttg ctttcatgcc gccagctgct cgattcagtg tagggatcct
    26821 tttgatcatc gctttacacg cactgaatat gtttttgaca ctgctaagtg cctatgtcca
    26881 tggcgcacga ttgcagtatg tcgaattttt tgggaagttt tatacaggag gcggacgtgc
    26941 ctttgatccg ctgaaaacgg cggaaaaata cgtcaacatc aatcacaaga aaaaaaacaa
    27001 taaataattg gaggattttt atataatgat ggattactta attagtcaaa atggtggaat
    27061 ggtatttgcg gtattagcga tggcaacagc gacgattttt tcagggatcg gttcagctaa
    27121 aggtgttgga atgacaggtg aggcagcagc agcattgaca acaagtcaac ccgaaaaatt
    27181 cgggcaggct ctgattctgc aattgcttcc aggcacgcaa ggtttgtatg gattcgttat
    27241 tgcctttttg atttttatca acttaggaaa tgacatgtca gttgtacaag gactgaactt
    27301 tctaggtgct tctctgccga ttgcttttac aggcttgttt tcaggtattg cccaaggaaa
    27361 agtcgcagcg gcaggtattc aaattttagc gaaaaaacct gaacatgcta caaaaggaat
    27421 catttttgct gcgatggttg aaacatacgc gattttaggt ttcgttattt ccttcctatt
    27481 agtgttgaat gcctaagcat aaaggaggaa aagcagatga acgccattga aaaaatcatc
    27541 tctcagatga acgaagcagc agagcaggaa agagcggcgt tagaacaaga agagcgaatg
    27601 aagatcgatc aaaactttga acaaaaacgt acgcaagttg aaacagaaca tcaaaaacaa
    27661 aaagaaaaac aaatcgaatt actcgaaaaa aaataccgtc agctaaggaa tcgccagcaa
    27721 gtagaagtac gacaagaaaa tttgaatgcg aagcaggaat ttttaagacg attgtttgca
    27781 gatgcggtaa ctgaaatgga aaattgggat gaatctgaac aaatccagtt tatcaaaaat
    27841 gcattgtatt cattaccttt gacaggaaaa gtagcattca ttgctggaga aaaatcagca
    27901 gcctatcttt ctcagacgtt gttggatgaa tggaacaatg aacttccctt tatgatggtt
    27961 ttaagtgatg aaactgttgc tgatcaagcg ggttttctga taaacgatca aggagttcag
    28021 tataattttc ttttcagcag ccttgtacaa gatatccaag gaacgatgag ttttgagatt
    28081 gccaatcaat tattcgaata aggagggatc agtgtatgga ataccacgaa ctaaatccct
    28141 tgattcgcgg aagagagctt gaacttttgt ccaatgagac atttgaacgg atgattcaaa
    28201 caaattctgt cgaagcactt ggtgaaatat tgaaatccac gatttacagt ccatatattt
    28261 atgacggata tgaatatgac ttcgaacaga atcttttaaa ggagcagagc cgtctatttc
    28321 gctggttaaa ggaatcagca ccggagcctg aaatcgtttg gatctatacg atgcgttata
    28381 ctttccataa tctgaaggtt ttgacaaaag ctgagatgac aaatcaaaat ctggaccatt
    28441 tgtatatcga cgatggattt tattcattag aaaccttgaa ggatgccatt catacgcaag
    28501 tttcaaatga acttcctgaa agcattatgg acagtatacg agaagtacgt gattattttg
    28561 aagagtctac cattcttcag ggaattgacg tgatttatga tcgacagttt ttgacagagc
    28621 agcgacggct tgccgataag ctaggatatc aagaattgct gacagaagtc attgctttta
    28681 ttgacttgac caatatcaca acgatggctc gaggaatatt gcagaaaaga acatatgggt
    28741 tcatgacgac ggtactttcc agctcgggaa gtatcgaaaa agaagtattt ttgaactttg
    28801 tagataaaga tatggaagat tacacgaatt ttctattaga gacagattat gctgattttc
    28861 tgcgccctgt catccgtgag ggaaatattg atctcgtaaa gcttgaacag ttgaaagatg
    28921 attatctaag ttcattgtat caagaagcac aaacccaggc tttcggacca ctgccactac
    28981 tagccttttt gaacgcaaaa gaggtagaaa gcaaaaacct tcgtttactg gttatcggca
    29041 aaagaagcca tttcgaatca gaaacaatca gaaagcgggt gagacaagtc tatgacgcat
    29101 aagatcggtg tagttggcga caaagattct gttttgccat tcaaattgtt tggctttgat
    29161 gttcgttatg gtacaaccaa gcaaaccgtt cgtcaggcaa ttgaagaaat ggcaaagaat
    29221 gagtatggcg tgatttatgt cacagagcaa tgtgcagcat tgagcccaga aacgatcgaa
    29281 cgttacaagg aacaaatgat acctgctatc gtattgattc ccaatcatca aggatcatta
    29341 gggatagggc tggaagaaat tcaaaaaaac gtagaaaaag cagtaggtca aaatatctta
    29401 tagtatgaaa aggagataga aattttgcaa attggaaaaa tcataaaagt ctccggacct
    29461 cttgtaatgg ctgagaacat gtctgatgcc agtatccaag acatgtgtct agtgggagat
    29521 ctaggagtta tcggagaaat tatcgagatg cgtggcgatg tggcgtctat tcaagtatat
    29581 gaagagactt ctggaattgg tccaggcgaa ccggttcgct ctactggtga agctttatct
    29641 gttgaattag gaccagggat catttcacaa atgtttgacg gaatccaacg gccattagat
    29701 acctttatgg aaatcacaca aagtaatttc ttagggcgcg gagttcaatt gcccgcattg
    29761 gaccatgaga aaaaatggtg gttcgaacca acagtagaag ccggagaaac ggtgagtgct
    29821 ggagatgtta tcgggatcgt agaagaaaca aaagttataa aacataaaat catggtgcca
    29881 aatgggataa aaggaacgat caaaacaatc gaaaaaggtt cattcactat tgatgaaaca
    29941 gtatgtgtca ttgaaaccga aaatggtgac aaaaatttga cgatgcttca aaaatggcca
    30001 gtaagacgta gtcggccaat caaagaaaaa ttaaacccgg atgcacccat gatcactgga
    30061 cagcgagtga ttgatacatt cttcccagtg acaaaaggag gagcagcagc tgtacctggt
    30121 ccttttggtg cagggaaaac agtcgttcag catcaaattg caaaatgggc cgatgtggac
    30181 atggttgtct atgtgggatg tggcgagcgt ggaaatgaga tgacggatgt tttaaatgaa
    30241 ttccctgaat tgattgaccc taatacagga gaatcgttga tggaacgtac ggtattgatt
    30301 gctaatacat caaacatgcc agtagcagct cgagaagctt ctatctatac gggaatcaca
    30361 attgcggaat atttccgtga catgggttat gacgttgcta tcatggcaga ctccacttca
    30421 cgatgggcag aagcacttcg tgagatgagc ggacgtttgg aagaaatgcc tggtgatgaa
    30481 ggttatccag cttatcttgg ttcacggctt gcggaatact atgaacgatc tggaagagtc
    30541 attgcattag gatcagaagg tcgcgaagga agtataaccg caatcagtgc tgtatccccg
    30601 tctggagggg atatttcgga accagtcaca caaaatacat tgcgggtcgt aaaagtattc
    30661 tggggtcttg attccagttt agcccaaaaa agacattttc cttcgatcga ttggatacaa
    30721 agctattctc tttattcaac agaagtaggg aagtatatgg atcatatcct tcaagctgac
    30781 tgggcatcca tggtcaccga gggcatgcgt attttgcagg aagaacagca gctgaatgaa
    30841 atcgtgcgtt tggtagggat tgattccctt tcagataatg accgtctgac attagaagtg
    30901 gcaaaatcca ttcgtgaaga ttatttgcag caaaatgctt ttgatgatgt cgatacattc
    30961 acttcaagag agaaacaatt caaaatgttg aagatgatct tgacctttgg tgaagaagga
    31021 cgccgtgcgt tgtcgttagg tgcttattta agtgagatca tggacggtac cgtatcgatt
    31081 cgtgaaagga tcggacgaag caaatatatc ccggaaacag aattagaaaa actagatcaa
    31141 attattggtg aaatcaagca aacgatgcaa acgatcgttt cagaaggagg catgagcaat
    31201 gattaaagaa tatcgtacga tcggtgaagt cgtcggtcca ttgatggcgg ttgaaaaagt
    31261 tgctggtgtc aaatatgaag aattaatcga agtccgtatg caaaatggcg agattcgtcg
    31321 tggacaagta ttggaagtcc aagaagataa agcaatggtc caaatattcg aaggaacaag
    31381 tggtatcaat ttacgtgact cttctgtacg atttttaggc catcctttag aactaggtgt
    31441 atcggaagac atgatcggtc gagtattcga tggtttggga cgaccgaaag ataacggacc
    31501 agagatcctg cctgaaaagc atttggatat taacggagaa gtaatcaatc cagtagctcg
    31561 ggattatcca gatgagttta tccaaacagg tatctctgca atcgatcatt tgaatacatt
    31621 agttcgtggg caaaaattac cagtattttc tggctctggt cttcctcata aagaattagc
    31681 tgctcagatc gcgcgacaag caacggtact cgattcatca gatgatttcg ccgtagtctt
    31741 tgctgctatt ggaatcactt ttgaagaagc tgaatttttc atggaagatt ttcgtcaaac
    31801 gggcgcaatc gaccgttctg tcatgtttat gaatttagca aacgaccctg caatcgaacg
    31861 tatcgctact cctcgtatgg ctttgactgc tgcagaatat cttgcttatg aaaagggaat
    31921 gcatgttttg gttatcatga cagacatgac caactatgca gaggcattgc gagagatttc
    31981 tgcagcacgg cgagaagttc caggaagaag aggatatcct gggtatcttt acacgaactt
    32041 agctacttta ttcgaacgag caggaagaat ccgcggacta aaaggatcgg ttactcaaat
    32101 tccgattcta acaatgccag aagacgataa aactcatcca atccctgact tgactggtta
    32161 tattacagaa ggacagatca tcttgactag agagctttac aagagtggga ttcagccacc
    32221 aattgatgta ctcccgtctc tttcgcggtt aaaagataag ggaactggac ccggaaagac
    32281 aagagaagac catgcagcta cgatgaatca gctttttgca gcctatgcac aaggaaaaca
    32341 ggcaaaagaa ttagccgtag ttttaggtga atctgcttta tctgatgtag acaagatcta
    32401 tgcgaagttt gctgagcggt ttgaaaaaga atatgtcaat caaggattct acacgaaccg
    32461 ttcgattact gaaacacttg atctagggtg ggaactgcta tcgatgttgc caagaaccga
    32521 attaaaacga atcaaagacg acatgctgga caaatatctg ccagaaggga agtgagttct
    32581 gatggcacga ttgaatgtga atccgacccg catggaattg actcgattga aaaaacagtt
    32641 gacaactgcc acaagagggc ataaattatt aaaagataag caagatgaac tgatgcgtca
    32701 attcatcctg ttgattcgaa agaacaacca gctgcgtcaa gaaatggaag agtctatgac
    32761 agaagcaatg tctgattttg tattggcaaa tgcttcgatc acggaacctt tcatcgaaga
    32821 attatttgtt ttgccggcgg aaaacgttga tttgtctatc gtagacaaaa atatcatgag
    32881 tgtaaaagta cctgtgatga attttgaata tgatgaacag ctgacagaca cacctttgga
    32941 atacgggtat ttgaattcca gtgccgaact ggatcggtca atcgatcggt ttactaccct
    33001 gcttccaaaa cttttggaac tgacagaaat cgaaaaaact tgtcaactga tggcgagtga
    33061 aatcgaaaag acacgtcggc gagtcaatgc attagaatat atgacgatcc ctcaattaga
    33121 agaaacgatc tattatattc gtatgaagct ggaagaaaac gagcgcgcag aagtaacaag
    33181 gttgatcaaa gtaaaaaata tgggtaccac tgactaagtg gtatcttttt ttaaaagagg
    33241 aaaacgatga ttaaacaaag caaacaaata aagaaacgtt tgccggctgt tcagcttctc
    33301 gcattaggat tttttctcct gattttagtt ggcggcggcc tacttaccct accatttttt
    33361 agtaatagtg ggcatgggac aaattttatc gatgcgcttt tcactgcaac ttctgctgtc
    33421 tgtgtaacgg gattgacaac attgaataca gcagagcatt ggaacagtgc tggtcaattt
    33481 ttgatcatgc ttttgattga gatcggcggt cttggtttta tgatggttcc gattctatgc
    33541 tttgcattga taaagaagaa agtcagtctc agcacacgta ttatattaaa agaagcgctg
    33601 aatctagaag aagtatcagg tgtgatgaag ctcatgcttt acatcctaaa attggccttt
    33661 gtgatccaat tgattggtac ggttgcttta tcttttgtat ttgtgcctga atttggctgg
    33721 ttgaaaggta tctggtacag tttgttccat gcaatctcta gtttttgtaa tgcagggttt
    33781 gatttgttag gagacagttt agccaatcac cagcaaaatg tttatttgat catggtggta
    33841 tctgctttga tcatttctgg tgggttaggc ttcatcgtat ggcgtgattt actggaatat
    33901 catcagaaaa agaagatgac gattcattca aaaatcgcat taaccgttac aggtaccctt
    33961 ttattaggcg gattcgttgt gttctttttc acagagcgca atggggcaac gctagtggaa
    34021 gggaatatgt ttcaacgatt ggcaaacacc ttttttatga gtgtgacacc tagaacggct
    34081 ggatattatt cagttgatta cctccatatg agccatgcag gtctgatttt aacgatgttt
    34141 ttgatgtaca tcggcggaac atctgggtcg acggccggcg gattgaaaac aactacgtta
    34201 ggtgtgttgc tgatccagat gcgttcgatg ttgaaaggac gtacgagagc ggaagcattt
    34261 ggccgtacca tccgacaagg cgcagtgttg cgggcactta ctttattttt tgttacgttg
    34321 acgttgtgtg tagtcgccat catgatttta tcggtgactg aaacgatacc taaaacttct
    34381 ggcatcgaat atattgcttt tgaggtcttc tctgcgtttg gtacagtagg tcttacgatg
    34441 ggattgacgc cagacttgac tgcaataggg aaattgatca ttatagcgct tatgtacatt
    34501 ggacgagttg gtgtattgac tgtggtcttc tctctgatga caaaagcaca aagaacacaa
    34561 tcaaaataca aatatccaga agaaagtgtc atgattggat aatttcatct tctgacagaa
    34621 acctagtgat ttagtaaaat aagaaaataa aataattttt cttgcttctt ggatacttta
    34681 agtgcaaaat gttcgtttat tttttgaggc actacccgtt gttgaacctc gggtagtgcc
    34741 tcttttttat tgaaaggaat caataagatt tgattccttt atttatcaat aggttataat
    34801 tcatattttt ttattttttc ttttacgata cttagtccag aagctaaaat ttcaaggtta
    34861 ttagaggata gggaaattcg aacgaatgca tctggttgcg cacgtgtagt aaagcgtctg
    34921 gaatgataaa gtcggatacc gttagcaaga aagtcattct caattatctt agggtcaatc
    34981 tcaggatcaa ctggaagaat tcgatagtaa gggacacgat tcgttggttt ttctactttg
    35041 aaaagagaat caaaaagctg attggctctt tttgtaagac ttaatttagt ttctaagatc
    35101 ctatttgctt catttgaatg gaggatttga gtgatgactt ccacatctaa tccagaagtt
    35161 ttcacattta tattaaaaat tgcttgttct atcttgcttt ggacatgtac tggaactaca
    35221 agataagcaa ctcgcaatcc agaacaaata aattttgtca ttccagctaa gtagattgtt
    35281 tggttaggta ataaattttg gaaaggagga atgatcgtat gttgatagta agttgttaaa
    35341 aatgaatgga tatcatcttc aataacccat agaccttctt tttcaatcac ttcggctaat
    35401 tgctctcgtc ttttgtgaga gatttgaaac cctataggat tattacaaga aggcataaga
    35461 aagacaccat gaatcttctt ttttctacat tcctgaatca atatatcagg tcgcattcct
    35521 tgctcgtcaa aatcaattgg aacgatctct aaatgatata actgagccaa ttcaatgaaa
    35581 ttagcgtatg tatatcggtc tgttgctatt ctatcacctg gagagaaaag tgcagaaagt
    35641 gtgatagcca atccattttg aacaccggaa acaatagata gagattctaa ggacggaatg
    35701 actccttgag tttttaacca atttgctgca gtagtcaatt gaaaatttgt gcctttggga
    35761 tcttggtaat taagcaaacc aacaagattt gggttattac tagttgattg aataaacggg
    35821 acaacgaatt tattacattc ttcaaaagaa ctaaccagac caaaatcaat gatatgttcg
    35881 gttacttcat tggtagagat ggtgatggac tcaaagacat tgggagaaat aaatgtgcca
    35941 cttccaatat tcgtatacag caaacctttc tcaattccat atttataagc cttgccaacg
    36001 gtagtaaaat ttaaatctaa ataatcagct agctctcttt gggagggtaa ctgagtgttt
    36061 ttctgtaatt tattctttaa aatatcttgt tctaattgtt gaatcaacga tcgataataa
    36121 ggacgcttca atttttgttt gtctggtcgc caagttaacg ggtaattgtc ataagaatta
    36181 ataggcattt tctccactcc ttaaaattgt ataccataca attttaccat tgattgtatt
    36241 gattttagaa gttaatcttt tgtgaggagg gaattgtcta tgaaaattat taattttgag
    36301 gaagtagtag cttcttttag ttttgaagaa gcgattcaag taatgaaaaa atgttttttt
    36361 gattatgaaa gtggtaaaat cagccaaaat cctcgattgg tggaaatttt acctgacgga
    36421 aaaggcaaaa atatgtttgc gatgatgcct gcttatttgg gggagaacag atattttggc
    36481 acaaaagtca ttaccgcttt tccggataat catcagtatc atttaccttc tcatttagga
    36541 gagattttct tgtttgatgc tcaaaacgga ctacccaaag cattgataaa cgcaaatgca
    36601 gtcacctgga ttcgaacagc tgctgtatct gcgctggcaa cagattactt agcaaatccg
    36661 catgcagaaa cacttgcttt aattggagct ggtcaacaag cattgtcgca tttgactgcg
    36721 atttctacca ttcgtcccat cactacagtg tttgtttacg atattttaga agaaagacga
    36781 aatactttta tcgctaaagc taaaaaacag tatcctaaaa taacgtttat aaatgcaata
    36841 tcagtagaag atgcagtacg tccagcggaa atcatttgta cgttgacctc tagtaaagag
    36901 gcatttcttc aaaaagagtg ggtaagggaa gacgcacata tcaatgctgt gggcacattt
    36961 actcctgata caagagagat aagtacatcg ttgatggaaa cttctcaaat ctatgtagat
    37021 gattactcag ctgccttaac agaaagcggg gacctgctta tagccatggc agaaggagat
    37081 ttagaggaat cttgtattgt tggtacatta ggcgaattag tttctgagaa aaaaacattt
    37141 tataaaggag aatcgaggcc gacgattttc gacgcagttg gtctagctat tgaagatctt
    37201 tgttgtgcag aatatattta taacaaatta gagaaagggg ctttaaaatg aacttagatt
    37261 attacgtagt tgatgcattt gcagatgagg tgttcaaagg aaatccagca gcggtttatg
    37321 tgctagaaga atggctgcca gaaggtacga tgcaaaaaat tgccattgaa aacaatttat
    37381 ctgaaacagc atttacagtg aagaaaaacc aagaatttga gcttagatgg tttacgccag
    37441 atcgagaaat cgatctttgt ggtcacgcga cattagcaac agcatttgtt ttatttaact
    37501 attacaaaat acctgatgaa acgattaaat tttctactca aagcggtaat ctatttgtga
    37561 caagaattaa agattattat tacatggatt ttcctagtat tatgccgaaa aaagtgccga
    37621 tattgtctga atatgaagaa gcgattgggg ctaagattaa agaagcctac ttagcacgtg
    37681 atttattttt cgtattagaa gatgaaaaaa cggtggctaa attgcagcca gattttacag
    37741 ctatcaaaaa ttttgaatta ggaataggag tcatcgtgac agcagaaagt gtgcaaaaag
    37801 actttgtttc tagaaccttc tttccaaaat taacaatcaa tgaagatcca gtatgtggat
    37861 cagcacattc taatcttatt ccttattggg cagaaagaat gaataaaaca aaattgtcag
    37921 cttatcaaat gtcatcaagg ggcggggatc tagaatgtga attaaaagaa gatcgtgtga
    37981 taataggagg aaaagcagtt ttgttttcta aaggtacctc gtttatttga tttctttcaa
    38041 atgaattaaa aataaattgg tggagagtgg caaccttata gctacaatgg ttgttaaaaa
    38101 aagacgcaaa ctttttcaat ttaatgaaaa gtttgcgtct cattttaatt attggtgttt
    38161 gttttttatt caatcaagag taaaatgccc catactaact atcctcggat gacttcgatt
    38221 ttataaccat cagggtcaat gacaaagtag taagaaggcg cagtgccagg taatcctttt
    38281 aagtcagtaa tctcaagacc agcagcttta tgttgctcgt gtaatttttc tagatcatca
    38341 gctgctatag cgatatgacc atagccgtct cctagatcat acgcaccatg atcatagttg
    38401 taagtcaact ctaattcata gtcatcccca ggaagtgtca aataaacaag tgtaaatttg
    38461 ttttctggaa aatcacggcg gcggctttct tcaaaaccaa atgccttttg ataaaattcg
    38521 attgatgctt caagatcttt cactcgaaca catgtgtgag ccattttcat agattcatct
    38581 ctccttttcc ttttactcaa tcataacaaa aagaagtaag gaaatagttg atttgtgctt
    38641 gttcatgaga aatttctcat gtaaaatgga tagaaaataa aactgttaag gtggaaacta
    38701 ttcatggata aaccaatttt tggtgaaaaa aagcaatttg ctgattatca aacccgttat
    38761 gcagcatata ttgtcataga aagagatcag cagattgcag taatcgaggc tccaaatggt
    38821 gctttttttc ttcctggcgg ggaaattgaa ggagaagaga cgaaagaaga agcgattgtc
    38881 cgtgagctga tagaagaaat gggaattgtg gcagaaattt cttcttatct gggagaagct
    38941 gatgagtatt tttactcgaa ttatcgtgca acttattatt ataatccagg atatttcttt
    39001 gttgctgatt cttggaagcg tataggagaa ccaactgaaa agaccaataa aatttggtgg
    39061 gtgagccctc aagaagcgct tgtgaaattg aaacgtggta gccaccgatg ggcagtcgag
    39121 agatggatcg aaaaaaataa aaactgaaag aaagcgagta gatcagcttt ctctcagttt
    39181 ttttatttat tacagtacat attttttgat tgcttcagct acaccgtgct cgtcgtttga
    39241 tgaagtgatg acatttgctg cttctttgac aagagaaacg gcatttccca ttgctactcc
    39301 tagaccagca tattcaatca tggataagtc gttctcgtta tctccgatag ccatgatttc
    39361 atcttgatca ataccaaggt gctgtgcgag attcgcaact gccgcacctt tacttgcttc
    39421 tttatttagt acttcgaagt aatagggcgc acttttgacc gttgtgtatt tatcacggaa
    39481 agtttgcggt aaacgcgcaa ttgctgcatc taaaatttct ggttcgtcga tcatcatcat
    39541 tttgacgatg ttcatgtctg gtgtcatttc ttcaggtgtt cgatatttta gcggcatctt
    39601 gactaaactt gcttcgtgga tcgtataagc gctgatatct cgattaggcg tgtagatcgc
    39661 tttgtcatcg atcgaatgga gatgactgcc gacacggcgt gccattactt cgatctctag
    39721 gaaatcttca tgtgtcagtc cgtgacgggc gatgatttta cctgatttcg tttgttgtac
    39781 gagtgccccg ttataagtaa taacgtaatc attatcttga aaaaggttca actcgtttaa
    39841 ctgttcctgg acacctggta agggacggcc agtgcataaa acgatcgaaa caccttttgc
    39901 aacagcagcg gcaatcgctt cttttacttc ttttgttact tctcttttct catttaataa
    39961 agtaccatcg atgtcgatag cgactagttt gatagacata atttcctcca atatatttta
    40021 atttatttca atcaatgcgc cattacggat atggctggca aattcttgat aagtttcgtc
    40081 aaataaatca taatgatcac gcaaggacgg atcaaccatt tcttttggaa agaaaaatcg
    40141 ctcatctcct tgttcttgtc ccgctaatgc agcaacaagc tgactaacct gtgataattc
    40201 tatcaaagtt ccgtcatttc gcaaaatctc gatttgtgtg cgatgtctgc cttgttctgg
    40261 gcgataaaaa tcataaggta gatcgtagct agaattcaca gcagtgtaat aaacaggatt
    40321 atatccgacc ttttctacta acaaagtgag ttcttggact aattttgaat cccggttgcc
    40381 agaaaaagta gcagatttca gtggtttacg attcaaaaaa cgtttagcta aatcatttaa
    40441 aataggatca gcagattcag accattgagt aaagtaagtg cccaagaccc catcatcaag
    40501 tttgaggtat tcttctaaag taaagcttcc gtcaagaaat ggcagaagta attgagaatg
    40561 gagttcaaaa ctgctggcat cttcttgata caactcgtgt gcgcgatgaa gcagatgctc
    40621 caaaattact tccataccac gggaaacagg atggaagtac acttgcacat acatttgata
    40681 tcggctaacg atataatctt ccactgcatg catgccactc atggaaaaag cgatcccgcc
    40741 ttcataagga cgaatcacac gtaatattcg ggtaagatca aatgttccat attctgttcc
    40801 agtgaaataa gcatcccgaa gcaaatagtc cattcgatct gcatcgatct gactagaaat
    40861 catttggaca acttgtgggt tggggtaagt tttttggatc acactagcta ccttttcagg
    40921 aaaaccttct tctactcggt taagaatctg atacacttct gtttctggag aagtgatgat
    40981 ctctacagtg atggcttcat gattcgtatg gaagatatgt tcaaatgtat gagagtaagg
    41041 accatgaccg acatcatgca acagtgccgc acataaagtc accagccgtt cctgatcatc
    41101 ccagcctccg ttgcctattt tttcttttga aaaatttcga gaaaagatat cgcagattcg
    41161 tcttgaaatc tcataaacgc ctaatgaatg agtaaaacgg ctatgttctg ctccgtgaaa
    41221 agtgaaagaa gaagtgccta attgtttaat gcgtcgtaga cgttggactt ctttcgaatt
    41281 gatcaaatct aagattactt gatgctggac atggatatag ttatggactg gatcacgaaa
    41341 aactttttcc ataggcagca tttgatattt ataaggggtt gtcataatgt agctccttta
    41401 tgttattacg ttgttccatt ctctggattt ggttgttcca ttgctccagc tggatttctt
    41461 tttttagcca gtcggtagaa tcctgtaaat cacttgcaga atacagcttt tcaaaagctt
    41521 ttgcaaatgc ttctttgact tcttttacaa aaaggggttg gtcaagcagt tcttcaagtg
    41581 ttgccattgt agcaggatcc actgcaggat agccatttgt accaaagtct tcttttaaag
    41641 cagattgata aaatcgcttc atgacttgcc cacgtttttc ctgattaccg ttgacactta
    41701 agtacatcat aacagaaatc ccatttttga tccgacgctg agcgataccg gcaaattttt
    41761 tgccatggat gcttaaatca taagttccag gacaataaga atgggtgatt tcgaatgctt
    41821 gtatcggtgc ctttggaaaa gcgtatttag tcaataggag catttgctcg taagcgtcat
    41881 ctatggttat tttttttgtg tcactttggg ggaaaatcaa agaaatattc aaaattcctt
    41941 catctgatac gatccctaat ccacctgaat ttctgatcac tggagcgtaa cctgcatctt
    42001 ccagtgtttg gatcccgtca cttagaaaag gagttcgaac atccttcata cctaagatga
    42061 ctgtgcagtc atattgccag aaatgtaaaa agatttgctg gtgttcttta gaatattccg
    42121 ttaatatatc tgttaatgca aaaggggaaa agtcgttttt ttgataaatg tgctgatcta
    42181 gtcgaatcgc agggttcatt caaccggctc ctttgtataa attcatattt tctacaatat
    42241 tatactctaa aatcaaagag ttgttttgga aaaaaggttt tctgtaaaaa tggacaagtt
    42301 ggttgacaaa acaaaagaaa ctaggaacaa tgtagctatg agaagtaaag gagaatgaat
    42361 aatggaatta tcacaaggaa caccgatttc aatcaaattg cgtacaaaag taaaacagca
    42421 aggggaagtg caagattttt attttgattt gaaagggcaa atggtgacaa ttggcgatac
    42481 gctgtacata cgttataaag agattcagga ggagacgggc gatgagattc ctgtaacgat
    42541 caaattagtt ccagatggtc atgtacagct gatccgtgca ggagaaatgc ggatgcgttt
    42601 gaaatttggc tataaagaac ggttagaaac atcctatcga acaccatacg gtatgatcca
    42661 gattgctact tttacgaaag aactgcatgt tagtttgaaa gaccgtccca cagcagggaa
    42721 ggttcgaatc gattatgatc tgttcatggg accggaaaaa atcggcgaat attatttgac
    42781 cttggatttt acagcgtaaa tataaaagaa aagtttgatt ttttgtgtct gattttgtat
    42841 gataaggagt agactgtgaa aggacgtgtc ctgattggaa attagtgtat ttgaaggtgc
    42901 aaacaaaagc gaattatcaa tgatcgaagt agcacacgcg atcttagaac aacgtggcga
    42961 tgtcatggat ttttctgatt tagctaacca gatccaaaac tatcttggga aatcagacag
    43021 tgaaattcgt gattcactcg cacaatttta tactgacttg aatattgacg gcagctttat
    43081 ctcattaggg gataatcgtt ggggcttgcg ttcatggtat gctatcgatt cgatcgatga
    43141 agaagtaaac catggaatgg acgaagagga tgaagatact ccacgtcgcc gtaaacgcaa
    43201 gaaagtcaac gcctttatca atgatgatga agacgcgatc gattacaatg acgatgaccc
    43261 agaagataca gatctgacag aagaagatga cgatgatctg tttgatgacg atgatgatga
    43321 agatgaagaa atcgctgctt ataattctga cttgcaagaa atcggagcag atgacccgac
    43381 agatgatgaa gaagacttgc caggaaatat cgaagaagat ttaagtatca tagacgaaga
    43441 cgatgatgat gaagattatg aagaggaaga attttctgac tctgaaaaag aataaacatt
    43501 attttcttac gagaggttgt gacagagacg atcagcctat cagaataagg aggtgtgaca
    43561 cgattttt
//
LOCUS       DAYYME010000004        42984 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000004 DAYYME010000000
VERSION     DAYYME010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.004"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..913
                     /locus_tag="ACHBFW_000160"
     CDS             140..913
                     /locus_tag="ACHBFW_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316510.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(994..1563)
                     /gene="thiT"
                     /locus_tag="ACHBFW_000161"
     CDS             complement(994..1563)
                     /gene="thiT"
                     /locus_tag="ACHBFW_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFM9316511.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(1629..1731)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1737..2708)
                     /locus_tag="ACHBFW_000162"
     CDS             complement(1737..2708)
                     /locus_tag="ACHBFW_000162"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM9316512.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(2802..4175)
                     /gene="glmU"
                     /locus_tag="ACHBFW_000163"
     CDS             complement(2802..4175)
                     /gene="glmU"
                     /locus_tag="ACHBFW_000163"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFM9316513.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(4678..5496)
                     /gene="purR"
                     /locus_tag="ACHBFW_000164"
     CDS             complement(4678..5496)
                     /gene="purR"
                     /locus_tag="ACHBFW_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFM9316514.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(5663..6487)
                     /locus_tag="ACHBFW_000165"
     CDS             complement(5663..6487)
                     /locus_tag="ACHBFW_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM9316515.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(6460..7140)
                     /locus_tag="ACHBFW_000166"
     CDS             complement(6460..7140)
                     /locus_tag="ACHBFW_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM9316516.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(7163..8101)
                     /locus_tag="ACHBFW_000167"
     CDS             complement(7163..8101)
                     /locus_tag="ACHBFW_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM9316517.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(8346..9197)
                     /gene="ispE"
                     /locus_tag="ACHBFW_000168"
     CDS             complement(8346..9197)
                     /gene="ispE"
                     /locus_tag="ACHBFW_000168"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFM9316518.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(9351..11924)
                     /gene="mprF"
                     /locus_tag="ACHBFW_000169"
     CDS             complement(9351..11924)
                     /gene="mprF"
                     /locus_tag="ACHBFW_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM9316519.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            12065..12871
                     /gene="proC"
                     /locus_tag="ACHBFW_000170"
     CDS             12065..12871
                     /gene="proC"
                     /locus_tag="ACHBFW_000170"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFM9316520.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(12950..14506)
                     /gene="rny"
                     /locus_tag="ACHBFW_000171"
     CDS             complement(12950..14506)
                     /gene="rny"
                     /locus_tag="ACHBFW_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFM9316521.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(14917..15966)
                     /gene="recA"
                     /locus_tag="ACHBFW_000172"
     CDS             complement(14917..15966)
                     /gene="recA"
                     /locus_tag="ACHBFW_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFM9316522.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(16075..17316)
                     /locus_tag="ACHBFW_000173"
     CDS             complement(16075..17316)
                     /locus_tag="ACHBFW_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFM9316523.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(17556..18134)
                     /gene="pgsA"
                     /locus_tag="ACHBFW_000174"
     CDS             complement(17556..18134)
                     /gene="pgsA"
                     /locus_tag="ACHBFW_000174"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFM9316524.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(18343..19266)
                     /locus_tag="ACHBFW_000175"
     CDS             complement(18343..19266)
                     /locus_tag="ACHBFW_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFM9316525.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(20005..21291)
                     /locus_tag="ACHBFW_000176"
     CDS             complement(20005..21291)
                     /locus_tag="ACHBFW_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM9316526.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(21284..22552)
                     /locus_tag="ACHBFW_000177"
     CDS             complement(21284..22552)
                     /locus_tag="ACHBFW_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM9316527.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            22804..23265
                     /gene="mscL"
                     /locus_tag="ACHBFW_000178"
     CDS             22804..23265
                     /gene="mscL"
                     /locus_tag="ACHBFW_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFM9316528.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(23324..23770)
                     /locus_tag="ACHBFW_000179"
     CDS             complement(23324..23770)
                     /locus_tag="ACHBFW_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9316529.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(23863..24108)
                     /locus_tag="ACHBFW_000180"
     CDS             complement(23863..24108)
                     /locus_tag="ACHBFW_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFM9316530.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(24223..24801)
                     /locus_tag="ACHBFW_000181"
     CDS             complement(24223..24801)
                     /locus_tag="ACHBFW_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9316531.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(24878..25408)
                     /locus_tag="ACHBFW_000182"
     CDS             complement(24878..25408)
                     /locus_tag="ACHBFW_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFM9316532.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(25412..26272)
                     /gene="rlmB"
                     /locus_tag="ACHBFW_000183"
     CDS             complement(25412..26272)
                     /gene="rlmB"
                     /locus_tag="ACHBFW_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFM9316533.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(26265..26660)
                     /locus_tag="ACHBFW_000184"
     CDS             complement(26265..26660)
                     /locus_tag="ACHBFW_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFM9316534.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(26657..28069)
                     /gene="cysS"
                     /locus_tag="ACHBFW_000185"
     CDS             complement(26657..28069)
                     /gene="cysS"
                     /locus_tag="ACHBFW_000185"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFM9316535.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(28066..28602)
                     /gene="epsC"
                     /locus_tag="ACHBFW_000186"
     CDS             complement(28066..28602)
                     /gene="epsC"
                     /locus_tag="ACHBFW_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFM9316536.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(28688..28927)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(28924..30384)
                     /gene="gltX"
                     /locus_tag="ACHBFW_000187"
     CDS             complement(28924..30384)
                     /gene="gltX"
                     /locus_tag="ACHBFW_000187"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFM9316537.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(30554..31711)
                     /locus_tag="ACHBFW_000188"
     CDS             complement(30554..31711)
                     /locus_tag="ACHBFW_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFM9316538.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(31745..33115)
                     /gene="radA"
                     /locus_tag="ACHBFW_000189"
     CDS             complement(31745..33115)
                     /gene="radA"
                     /locus_tag="ACHBFW_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFM9316539.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(33178..33705)
                     /locus_tag="ACHBFW_000190"
     CDS             complement(33178..33705)
                     /locus_tag="ACHBFW_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFM9316540.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            33935..34741
                     /gene="proB"
                     /locus_tag="ACHBFW_000191"
     CDS             33935..34741
                     /gene="proB"
                     /locus_tag="ACHBFW_000191"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFM9316541.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            34738..35985
                     /locus_tag="ACHBFW_000192"
     CDS             34738..35985
                     /locus_tag="ACHBFW_000192"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFM9316542.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            36061..36405
                     /locus_tag="ACHBFW_000193"
     CDS             36061..36405
                     /locus_tag="ACHBFW_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316543.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(36522..36848)
                     /locus_tag="ACHBFW_000194"
     CDS             complement(36522..36848)
                     /locus_tag="ACHBFW_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316544.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(37463..38665)
                     /locus_tag="ACHBFW_000195"
     CDS             complement(37463..38665)
                     /locus_tag="ACHBFW_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9316545.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            38782..39297
                     /locus_tag="ACHBFW_000196"
     CDS             38782..39297
                     /locus_tag="ACHBFW_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9316546.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(39496..39942)
                     /locus_tag="ACHBFW_000197"
     CDS             complement(39496..39942)
                     /locus_tag="ACHBFW_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM9316547.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(39989..41467)
                     /gene="msr(C)"
                     /locus_tag="ACHBFW_000198"
     CDS             complement(39989..41467)
                     /gene="msr(C)"
                     /locus_tag="ACHBFW_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM9316548.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(41821..42450)
                     /locus_tag="ACHBFW_000199"
     CDS             complement(41821..42450)
                     /locus_tag="ACHBFW_000199"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM9316549.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(42434..42898)
                     /locus_tag="ACHBFW_000200"
     CDS             complement(42434..42898)
                     /locus_tag="ACHBFW_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9316550.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
BASE COUNT        12192 a         9295 c         7608 g        13889 t
ORIGIN      
        1 acaaaaatta attgaaaatg agagtgtttt caatgaaaac gtgattttgt aatggaattt
       61 taaaataaaa gaaacaagct tttttaatgc aaatgttgta aaataaaaga gattaagagg
      121 aaataagagg aggaacatga tgactgagtc agtttatgta catattgata ccacaagtaa
      181 tgctgtttta acaaaagggc tgactgctac tgactttgca aacagtatcg tgcattttcc
      241 tcaaaacttg ctcttgcttg atccatctgc ttctgctgga gaatatgaat cccatacagg
      301 gctgaaagta atccgtggta cagaaaatat tcagcgtttc ttttcctctt ctcgtaacag
      361 gcgtggattc gatgaattga agtggatcga ctttactgat ttaacgatgc tgaaagaatt
      421 gacaccttta gaaatttcag aattattata ttttggtcat atgaaaacac atcttcattc
      481 gccgtttttt tataaactac agaataattt tgtatacttc gatcttaatg atcagctgaa
      541 tcggatttat tatcgctatt tagatgaatt ttataggatt tttgctagta aattaagttc
      601 gatcttgtca gataagatca atgtaaaaaa atcttttttc aaaaaagaaa cacctgttga
      661 aaaaatcagt gtggaattat tgaaagaaat gcgtaatttg atgcaggaag ggatcatttt
      721 ttcttttgat caagttggtt tgatcgatgg taaatatcgt atcccaatct atgtgattga
      781 agattatatt tggaaattaa aaggcacccg gtatcgagaa gaggaaatca tcgctacttt
      841 aatctactct gtcaccgatc gacactggca attggtagaa gatgccgatc tttcttatct
      901 gccattgaag tagatctatt tcttatcaaa aaataaaaag gaaacaagaa actaggacat
      961 gattctgtct caagtttctt gcttcttttt tatttacagc tcctgtcgtt tatctggcac
     1021 aaaaatcgaa ggagtcgttt gttggatccc taataaaacg atgatcgtta caatagctgt
     1081 actcaacccg ctggcaccat tcattacgaa agaaaagagc caaggattca tcccccatag
     1141 tgcatacgct ccccagaaaa ccactcctgc aataaaatgc caaaaatatc tggaaagtgc
     1201 gccaatcaat gcaccaaaca cgatgctttt tctaatttgt ttcgtattat cgtctacata
     1261 gcctttttgg acatttgaag ccatgagccc tgctgcgcct gcaaaagtat aggcaatcgg
     1321 gtactcaatc aacacttgaa cgacacttaa ataataaact tgtcccaacg gaaaatgtag
     1381 gatgccccaa atgaaacctg ccagcatccc tgcctttgtc cctcttctta acgcaaaaag
     1441 agtcaatgga atctgcccta atgaaataga aaaactgctg ccgatctgga tcggaatcaa
     1501 cgacaacacc atcgctaacg ctgcaacaat cgttccttct acccaaactc tattttttcc
     1561 cataaaaaaa cttcctcctt tgaataaaaa agtcacaaag gaggaagcag ctttcgaaaa
     1621 tagtcaaaca gctgtttatt cacaattcct acgctcgcat aaccgataca ggtacgaagg
     1681 gtttagaaat agattcaatc tcagcccaat tggactcccc tttgtgagtt tatttattat
     1741 ttattttttg tttcaaacaa tggactaact ggtttgtttt catggatacg tttgattgca
     1801 tctcccatta gtccgccaac acttacttca tcgattttgt cgattttacg atcttctgga
     1861 agatagatcg agtctgtaac aactagacgc tcgatagctg aatcttctat tctttgtaaa
     1921 gcagggccgg ataatactgg atgtgtacat gaagcataca cactagttgc accagcttct
     1981 ttcaaagcat tggcagccaa tgtgatcgta cctgctgtat cgatcatatc atcgatcagt
     2041 acacaagttt tgcctttgac ttgtccaatg atattcatta cttctgcaac atttgctttt
     2101 ggacggcgtt tgtctatgat cgcaatcgga gatttcaaaa attccgctaa tttacgtgcc
     2161 cgtgtgacac caccatggtc tggtgaaaca acgacaacat catcgccttt gattcctttt
     2221 tctaagaaat aattggcaat aagtggcgca cccatcaaat gatcgactgg aatatcaaag
     2281 aaaccttgga tttggactgc atgaaggtcc aaagtcagca ttcttgttgc accagctttt
     2341 tgaatcatgt ttgctactaa tttcgctgtg attggttccc gtgcacgtgc tttacggtct
     2401 tgacgcgcat agccataata aggcatgact acgttgattg tttttgcact tgctcgtttc
     2461 agtgcgtcaa tcatgatcag caactccatc aagttatcat tgactgggct gctcgttgat
     2521 tgaataacat agacatgcga tccacgaata ctttcttcaa tgtttacttg aatttctcca
     2581 tcactaaatt gagtaacaga tgattttcct aactcgacgc cgactgctgc tgcaattttc
     2641 tctgctaatg ggcgattgga atttaacgca aaaattttta atcttgggtc aaagtaatgt
     2701 tttgacatgg ggacctccac tttctttttc acaactctag aattctacct aataaatagt
     2761 agtcatattt tttaaataaa tcaagtgatt ttgcaaaagt cttagtttag ataagggagt
     2821 ttttttgcat atccttcttt attgacttgt cttgctctcg caattgccag cgcatattct
     2881 ggaatattgt ctgtaattgt tgacccggca gcaatcgaac tgtttttagc tacctcgaca
     2941 ggtccgatga tattcgtgct agaaccaata aagctgtggt cgccgacagt cgtatgatgt
     3001 ttgttctttc catcatagtt gacgaagaca acaccacatc cgacattgat ttcttctccc
     3061 agcgtagcat caccaacata agtcaagtga ccgactttag ttcctttgcc gatttgagca
     3121 tttttcactt ccacaaagtt tccgatatgg acgttttcgc caatttcagc ttttggtcgt
     3181 aaatgtgcat aaggtcccac atctgcatgt ttcttgacat ggctgctttc aatgactgag
     3241 ttttcaatga cgacgtgatc ctcaatccta ctgtctacga tttttgaatg tgacccgatg
     3301 acacaatcag aaccaatgac tgtatttcct tggatttgta cacctgcttc gatcaatgtg
     3361 tctggaccga tttctacgcc tgcatcgatg tatgtcgttg ctgaatcgac aaagcttacg
     3421 ccattgacca tatgcatctg gttaattcgc tttctcatga tctcatttgc tttagctaac
     3481 gcgatccgat cattgactcc cattgattct tcaaaatcat ccgtttgata agcagcaact
     3541 gtatggcctt cttcttttaa gatttcgatg atatctgtta gatagtattc accttgagca
     3601 ttgtttgttc caactttgct taacgcatcg aacaaagctt cgttgtcaaa acagtaagtt
     3661 cctgtattga tttcttgtac aagagcttct tctggtgttg catctttttg ttccacgatt
     3721 ttttctacga tcccaatatg gtcacggatg attcgtccat atcctgttgg gttttctgct
     3781 tgtgccgtca agatcgttgc acttgcattt tttccttggt gatattcaaa aagattgttc
     3841 agcgtttcag tagtcaataa tggtgtatcg ccactgataa ctaacgttgt tccttttttt
     3901 ccttccaaga aagaagcagc ttgcaaaaca gcatgtcctg ttcccaattg ttctgcttgt
     3961 aacgcatatt tactgcgttc tcctagttgt gctttaactt gttctgctcc gtgtccaacg
     4021 atcgtaatca cttcatctgg tttcgtttca ctgacccgat taatgatatg ttctaccata
     4081 ggtttaccag aaacgggatg taaaacttta tataattttg acttcatgcg ggtacctttc
     4141 cctgccgcca aaataattgc ataacgtgcg tccaactgcg tcactcctaa atatagtttg
     4201 ctgatttcag tttatcccag tatacccatt ttttcaacaa aacaagtgat tttcttttat
     4261 cccagcgttt tctggataaa aaaatagata gaccataacg ggcctatcta tttggtaacc
     4321 ggtgctctcc tgccaactcc tcggtcacaa gtcacactac tcataaaaca tgaaaagctg
     4381 ttttttgaaa tcgtttcttg tgacttagag ttaaacggta ggcttacaac ctcttcatta
     4441 aacgtttttc aatcagctga ccttcggtat tagctcaata aacggcaaaa tatgaggagc
     4501 aatttttccg tttattgagc taatactcag gacaggacgc tgatttcaca acctctagat
     4561 aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgaaaa aatgtggaaa
     4621 gccattttca caaaattttt cttactactc gaaactaagc ggctttttca caacctctta
     4681 ttctgaaaaa taatttcctg gcacaaccgt aatatttttc gtttgagtat ctacatcttt
     4741 gacataaagc agtgacgtat aatcatcgat ggcgcgatga ccgttgaatt ttgattcggc
     4801 aaatacagtg atgccaacaa gttctgattc gaattcttca atcaagctct tcattccgtt
     4861 gactgttccg ccgcctttca tgaaatcatc cactacaagg acacgtgaac cacgtttcaa
     4921 acttctctta gacagttcca tcttttcaat tctttcagaa gatccagaca catagttgac
     4981 acttactgtt gatccttctg ttatttttga gtcccggcgt acaataacga atggcgcatt
     5041 cagataatag gacacagctt gggcaatagg tacgcctttt gtggctacag tcattacagc
     5101 atcgattttt tcaccaaggt atttagaagc aatgattctt ccaacttgac gcagcagttc
     5161 aggttctcct aacaaatcag agagataaac atatcctcct ggaagcagac ggtcttgttc
     5221 tgatagacgt tctgctagag attcaatata ttttttcgcc tcttcatatg aaatttcagg
     5281 gatgaacaat acaccacctg cagcacccgg gattgtttcc aaggttccat accctcgttc
     5341 tttaaatgtt ttcttgatga ttcccaaatc ttcactgata gaagatttag cagattcata
     5401 acgttcggca aagcttgtta aggaaatcaa ttcatgcggg tgattcaata aatactgtgt
     5461 catgtcaact agacgttcac tgcgacgtac tttcacctat tccaactcct tttaagaaat
     5521 tttatcgttt tacattatat aagtttttct tcaaaaattc gagcatttta tgaaaaaata
     5581 agaaaatagt tcggtattta ttaaaataaa aagaagccta taaaaaattt ttcgttttta
     5641 taaggcttct tttctttata atttagttat tttttcttct gaaggcaacc aatattcgtt
     5701 tgatgatatt tacaatcagg aacaaagcaa taaagactaa tgtgattgtt gctcctggag
     5761 gtgtatctag ataaaacgag ctagttagtc ctgacagcat ccctagaaat cctacacaaa
     5821 cactgatagc aatcacgaca ttgaagcttt tgcctaatcg cataccgatt gctgcgggaa
     5881 gaaccatgat tgcggatatc aaaagtgcac cggcaatcgg gatcatcaca gcaatagcca
     5941 caccagtaat cacattgaac atcatcgaca tcaaatgaac gggtagccca tccacgtggg
     6001 cggtatcttc atcaaaagtc agaacataca tcggccgttt gaataaaaag aacaatatac
     6061 cgatggcaac gaataagatt cctaaaaaga tgacttgctc ccatgtaatg gtcacgatcg
     6121 aaccaaacag ataggattgg atacttgttg ctgaattccc tccgctcaaa ttcatcaata
     6181 ctaacgctag cgccaaacca ccagccatta aaatggcaat ggagatttct gaataggttc
     6241 ggtagatcgt tcgcagatac tccaatatca cagctgctaa tacaacgact agcatggtcg
     6301 taagtgtagg attaaggttc aaaaagaatc ctaacgctac ccctgctaaa gagacatgag
     6361 aaagcgtatc agccatcagc gattgtcttc tgatcactaa aaacacacct agcatcgggg
     6421 cgattccggc aatgaagata gcggcaagaa acgctcttct catgaattca tatgaaagca
     6481 acgccattga gaatcctcct ttctcaccag tcggatctgt ctgtccgcat actgtttgat
     6541 atcttcatga tcatgggtga tcattagaac tgatttccca tggacatgcg cactatgtcg
     6601 gagaagacga taaaattcat ttctggattc ttcatccata cctgttgttg gttcatcaag
     6661 aacaaataag tcagggtcag tagcaaaaat acgcgctaaa ctgattcgct gcttttgtcc
     6721 gccagacaat tcacctattc gccgatttcg catatcccac atccctacag cttctaaagc
     6781 acgctgtaca tgttgatgat ctttttcaga gagacgcttg aaccagcggc ctcttggata
     6841 tcttcctgaa cggactaatt cgatcaccgt actaggaaaa cctgcattga aagatgcaac
     6901 ttgttgagga atataaccga tacttaattt ttttccttct tgatttttct cagcaatctt
     6961 tatcattcct tttgaaggtt tcagtaatcc taacgatgct ttcaccaagg ttgacttggc
     7021 tgctccattt tctccagtta aaatgacaaa ttcacccgca tctacatgat aagagacatc
     7081 ttctagtaca ggttcatcat catagtaaaa ggtcaaatca gatatttcga tataacgcac
     7141 cttacaacct ccattctaaa acttaattga tactttcttt taaagctttc agattttttt
     7201 ccatgacaga aatataattt tcgcctgctt tgatttgttc attagtcaag ctttctaaag
     7261 gattcaacac ttctagcttc actcctgttt cattcgacaa tgtctcagct accttcgaag
     7321 aggcattttc ttcaaaataa atcactttga tgttgttttt cttcacaaat tcttttagct
     7381 cagctaagcg acttggtgtg ggttcctgat ctggtgaaac gcccgaaatc gctacttgtt
     7441 ttaagtcgta ttgattcgct aaataagcaa aagcagcatg ctgagtgaca aatgtgcggt
     7501 ttgttgcatc ttttagctca tcggagtatt tttgatccag tgcttcaagc tttttgatat
     7561 aagcatcggc attcttcgta aaagtttcct ttttatctgg atatttttcg cctaattctt
     7621 cagcaatcgt tctgacttct tttatagcta ataccggatc gagccaaaca tgtggatcgt
     7681 attcatgtga atgaccatgc ccttcttctg attcttcatg ttcgtctgct tcttgcgatt
     7741 ctaaacggtc gattccttta gctgcttcga tcatcaaggt ctgtttgcta tccactgaat
     7801 cctttaacga atccacaaaa aattccatgt ctgaactatt atacacgaaa acatctgcat
     7861 cggaaatttc tgctctttct tttgcacttg gttcgaaatc atgcggctcc gttccagcag
     7921 ggatcaaaag tttcacgttt ccttcatccc ctacgatctc ttttgtaaaa tcatacatcg
     7981 gataaaaagt cgtaacgatc gtcaaatcct cttttttgtc tgcttccttg ttcgtatttc
     8041 cacatgctgc aaacagtaaa gcacctatca ttaccgtagc tgtcaaaaga tattttttca
     8101 tttatatgct cctttcaatg aacaaatcgg aattttacga tttattaacg tgaactaatg
     8161 tatcaaaata aaaaaacaca gtcaatagaa atccatcaat ttctgattta agcatgtata
     8221 ttcttgatta aatcgtaaaa tggatgagtt gctgctcata tctagccatt tcactacaat
     8281 aaaaaactga agaataccat tttaggattc ctcagttttt catcacctat tttacttctt
     8341 atattttatt gcagggttcg tactaaataa acttcctcac aaaaaccttt taacccatta
     8401 tatactctct gcgctcttga ataatgttga cacaaggcaa aaacggttgg tccgcttcca
     8461 ctcatcaatg cagcatctgc tccgtacttg atcatacgct ctttgatttg atggatgact
     8521 ggatgccgct ttcccgttat atcttctaaa ctgtttccca tttttttgat catctgctga
     8581 taatcttgct ctcggatggc ttcagcaagt ccgtggatgt cttgatgttg gatggcttgc
     8641 gtatctactt ctctaaacac tgtacgtgta gagacactca ttcttggttt gaccaacaca
     8701 acccagcact gtggcataga aggtaatact tggatttctt cccctttgcc accgacaaat
     8761 gccgtacttc cgtagacaca gtaaggaaca tctgtaccga cttccaaacc gatttctgcc
     8821 agttcttcta acgaacagcc cagattccat aattggttga tccctcttag agcagctgca
     8881 cagtctgtac ttcctccgcc taatccagct gctactggaa tattcttctt aacaaagatc
     8941 ttgacaccgg ttttgatatt gtatttattt tttagcaata aagcagcttg ataaacattg
     9001 ttccgttcat ctaccggtaa aaatgcttta tttgtttcta caatgatttt gtcttctgtt
     9061 atttcttcaa aaaatagatg atctgccaag tctacgctcg acatcaccat ttccaactca
     9121 tgatacccat cttgccgtct atataataca tccagtccta aatttatctt ggctggcgct
     9181 ttttcaatca attccatcct ctcacccatt cgaaaagtct aagtcgcaag tattctatca
     9241 tagggaaaaa taaaagtata gcttatgtct gcctagtggt tgattttgtc atcgtttcaa
     9301 cagctttcac agaattttct cttcataatc caaacaagtt ctagtgattc ttataattct
     9361 gtgagcactt cttctgccaa actcggaaca tttccttttt cttttcctat cagactgagt
     9421 agctgcatgg tagcaaatcc gaaattactt cctttcctat aagcaaaata acgtccttcc
     9481 caatgatcga catattttaa tttcttttca taagcagact ggaaggaata gaaaaaatta
     9541 ccataattat atacgatatg catgactttt tcttgaaaga aagaaaaatc tgtttctcca
     9601 acattagcta atggagctaa gcctagattg gcttccgagt agcccatttt ctcgtataca
     9661 ataaacagat tggctaacag atagtcagct aattcctcag attctttagg taagacccgc
     9721 aacaaatcat atcccgcctg cttcttcgtc atcggctgag cggtgatgaa gccaacgact
     9781 ttttgatctt tacggacaat accgacaccg ctagataata catatgattt atcaaatcgt
     9841 cccacagaga agtatttttc tcgtgcacct tgaagccatt tttgtgaaac ttcatttaca
     9901 gcttgatgta attcttccgg cagttgatcg taccaagtaa aatggtatcc ttctttcatc
     9961 aaacgttgat attcaagttg ctgactcagc cgagacaagt cttctgttgc tagatcaaca
    10021 atccctgctt cacctacttt cgtgaagtga aaacccaatt catgaagcat cactacgtag
    10081 ctttctgata tcttataaaa cgcaagctga tagcctaaac aatctgcttg tttcataaac
    10141 tgattcgttg ctgcccgcat cattttagga ttgccaactg gatctcctaa aacaaagcat
    10201 ttgtttcctt tgatctgata accaaaaacg acttgatctt ggtcgtcttc ttggtaataa
    10261 aaataactgt aacctgacaa gcgaagctga tggcttgtag ctgtgcctcc gtatgtttcc
    10321 aataaacggt caaaacggtc tttttcgtac tcactgccaa gcttcgtagt atcttcagct
    10381 aaatattgat aaagcgccac taaagctagc aatgagatgc ccaaaccaat aagccctgag
    10441 aaccacacat catctgaagg aaacaaaata aaatgatttc ctgttattcg accgactaaa
    10501 aagcgtccgc tgtgatagcc tgcaattgcg taaacgataa acaaaaagcc gaacaagcta
    10561 gcatcaaaga ttatagctcc ccaagaataa acgaattttg ttcgataaaa ttcttttctg
    10621 gataaccaca cagctaacag aatcaagaca tataccaata tcaactgcca agataacgta
    10681 cgtgtgattg tatttaaaat accaaaagat agtaaagcaa tcgtaggcag aaatgccttt
    10741 ttgactttta ctgaaagtgc tctagcaagg ccgagtaata aaaagccaac caatagattc
    10801 actgtctgat ctaaaaaatt gaatgagaac ggcaataata cttgaaatag ccgactgatt
    10861 gcagacagat tagtgattgt tgatagaagt accatcatga tccccgcaaa atagacagca
    10921 cttacgagca cgatatgcgc aaccttttgg aaaaacagtc gcggaatgtt atctaaaaat
    10981 cgattgattt tgactcctgt atgtgtgatg aacaatagaa gtcccgtcat aaaaggtaga
    11041 acataataaa acaaccgata atataatagc caaactactg ctacatcttg ccgaacagac
    11101 aattgactca ttcctaagat catcaaaacg tcaaaagtcc ccattccacc tgggaccatc
    11161 gtcagcattc ctatcaatgt agcaatcatg aacataggat agatcgaaag catcgaaata
    11221 tctattctca tcaacttgcc gatgatcaaa aataccgcaa gggctcccgt ccattgtcca
    11281 aaggatgcca gtagcagacc aagcaagcct tttggataaa actcgaagaa caatgtcttt
    11341 ctctttagat aagcaaaaaa gaaaatgact ggtgctaaaa aactaccgcc gaaaagccag
    11401 atccaatagc ttcgaaaaat actttctgac tgaataaaaa agacatcaat aaatgtaaga
    11461 aacgaccata cagacaaacc agtcagcata aatagtgcga cttttgatac agtggctaaa
    11521 accatttttc tatttgtctg ctttccataa aaactagccc gcagacttgc tcctaccaca
    11581 ccaccaaaac cggcgagatt gttgatcgtg tttgttaccc acgcagcaag aagaaactct
    11641 tttctttcca tttttggttt tccttgtttt tctaagactc tgatcgtcac ccaatcatac
    11701 aacaacatcg gagtgacacc cagcagtcca gccagaatca tgagcaggat cgtcatgcgg
    11761 ctttgttgtc ccatcctttg ccaaatatct tgccaactca tgccattagc aatatttgcg
    11821 acttgatttg ccacaaagac taacacggag ccgaaaaaaa tcagttttaa aatcagacta
    11881 tgttccttta tccattgcag catcaagtat cgtttatttt tcacattttc ccattcctaa
    11941 cttgtatact tttgtgtcat accccaatgt tatcttattt attttaattt ttccacttta
    12001 gacctgttct ttgcttgcga tgatagaaaa agaaagatat cctaatgcta aaggagagga
    12061 taacatgaaa gtaggattta tcggcacagg gaatatggca caagctatta ttgatggttt
    12121 attaaaaaaa ggaacagtct cgaaagaaga ggtgttcgtt tccagtggac attttgaaaa
    12181 tgcacaaaaa tttgctgctg aaactggcgt ccatgcatgc aaaagtaatc aggaagttgc
    12241 atcacaatca gatatcgttc ttttagccgt gaaaccaaaa atcattgaaa aagtattgga
    12301 agaactaaga gaatatagcc agaaagtctt attcatttct attgcttcag gaaaatcatt
    12361 aatagattta gaaacagcgc ttgaagatcc tcaggcctcc atcgtacgtg tgatgccgaa
    12421 tgtcaatgtc agcgtaggag caggcgtttc tgcgatttgc aaaaatgaag ctgccacgga
    12481 ggaacagctt cagcaagcta cgatgatttt tgaagcaatc ggcagcgtct atcctttagc
    12541 agaatcagat ttcagtacat tcattggcct tgctggcagc tcacccgcat tcgtttacat
    12601 gctgatcgat gcgatgggac ggactggtgt actgaatggt ttgtccaaac agacagccac
    12661 tgagattgct gcaaaagcaa ttatgggttc ttgtcaaaaa ttcctttcaa gtgataaaaa
    12721 tccatgggaa cttgtagacc aagtttcttc tcctggcggt acaactgttg caggtgtagt
    12781 tgccttagaa gaagcaggat tcatcccagc aatcatcaaa ggaatcaata cgacgatcga
    12841 gaaagatctt gagatgttga ataaaaaata atgatagcaa tgttttttcg tgccttacaa
    12901 aaaaagaaga gctgtggctt ttgccgcggc tcttcttttg tattttttat tatttagcat
    12961 aatcagttgc acgagtttcc cgaataacga ttactttgat gtgtccagga taatccagtt
    13021 cttcttcgat tcgtttacga atatcacgta ctaaacgaac agcgtctaaa tccgagattt
    13081 cttctggctt gaccattacc cgaacttcgc gtccagcttg aactgcgtaa cttgattcta
    13141 caccggcgaa gctgttcgaa atattttcca gacttcttaa acgacggatg tagttttcta
    13201 atgattcact tcgcgcacca ggtcttgctg ctgagagcgc atctgctgca gctacaagga
    13261 cagagataac agaagtagct tccacatctc catgatgaga agcgatcgcg ttgattacta
    13321 ctgggttttc tttgtacttg gcagctaatt ccgcaccgat ctcaacgtga gatccttcaa
    13381 tctcatggtc aagtgctttc ccaatatcat ggagcaaccc ggcacgtttt gctaattgga
    13441 tatcttcgcc aagctctgct gctaagatac cagctaattt tgcaacctcg accgaatgct
    13501 tcaagacgtt ttgtccataa ctagttctga atcgcagacg tccgatgatc ttgattagat
    13561 ctggatgaag tgtatgagca ccgacttcaa acgctgcctc ttccccgtat tcgcgaatac
    13621 gttcatccat ttctttacga gatttctcga ccatttcttc gatacgcgct ggatggattc
    13681 gtccatcttg tatcagtttt tccaaggtca tccgtgcgat ttctcttcgg attggatcga
    13741 atccggaaag aacaactgct tccggtgtat catcgatgat taaatcgata ccagtcagcg
    13801 tctcaagcgt tcgaatatta cgaccttctc gtccaatgat acgacctttc atttcatcat
    13861 tcggaagggt aacgactgaa acagtcgttt ctgaaacttg atctgctgcg cagcgttgaa
    13921 tcgctaatga taataagttt ttcgctttgc gatctgcttc ttctttggca cgctgttcag
    13981 attcttttac cattagagtt aactcatgat tcagctcttc ttcagtcgat ttcatgatga
    14041 catctcgtgc gtcctctttt gataaaccag caattttttc tagttctttt tgttgatctt
    14101 caatcatttt ttcaacgtct ttttctcgct cttcaattaa ttgctgtttt gaactaagtg
    14161 cttcttcttt actttcaagt gagttctcgc gtttttctaa cgagtcatct ttacgattca
    14221 gcgtttgttc gcgttgaatc aaacgattct cttgagattt taattctaac ttactttctc
    14281 tcagctcact ttcaacttca gaacggtatt tctgattttc ttccttagct tctaacaatg
    14341 cttctttttt tagagtttct gcttcttttt ttgcactatc gatgattcca gcagctgatg
    14401 attgtgcacc agctatttct ttctcgtgac gtgactttgc gaccataaac cctaaaccaa
    14461 gaccgacaat taaaccgatg atagcgagga gaatgttgaa taccataaaa tccacctcca
    14521 tactatcttt ttcattttta tcatctatac agcttctatg ataaattatt ctaaactcgt
    14581 caatatgcat cttcgcatat atgtgtacaa aaaacatgca caactttatt ctaatgtttg
    14641 taccccgtag tgtcaacttt aaataaacaa agaaacggct ttctacttat tcattaaacg
    14701 atggttttta aaataaatga cactagtttc atataggtaa acctttaaat tgagaaactt
    14761 ttctattaaa ttattctttg tagatgaaca gatattttta ttttatgtac ccactcgtat
    14821 aattaaataa ctcacgattt ctttattcaa taaaaaaacg gcaggtcaag gagagtacct
    14881 actcttcaga ccagccgttt ttgtttatca taatttttat tcttctacta aaggcaattc
    14941 ttcttgtcct ttttcatctt ctgggacagc gacttcttca ccaataccat aagctgctct
    15001 aactagagca gaaacttctg ccatcatttc aggatgatta gccatataga ttttagcgtt
    15061 ttcccgtcct tgaccaatcc gatcttcttt ataagaatac caagcaccgc ttttatcaac
    15121 aatgtctttt tctactgcca tgtctagaag ttctccttct tgtgagatcc ctaaaccata
    15181 catgacatct acttcagcga ttttgaaagg aggtgctact ttgtttttta cgactttgat
    15241 ctttgtacgg ttaccaacaa tatctgtacc ttgtttcaat tgttctgcac gacgtacttc
    15301 caaacggatc gttgcgtaga atttcaatgc gcgtcctcca ggagtgattt ctggattccc
    15361 aaacatcacg cctacttttt cacggatttg gttgatgaaa atagcgattg tttttgtttt
    15421 gttgatcgaa ccagagagtt tacgcaatgc ttgagacatc aaacgtgctt gtaacccgac
    15481 atgtgagtca cccatttcgc cgtcgatttc tgctcgtgga actaaagcag caactgaatc
    15541 gactactacg atatctacag ccccacttga tactaaagca tcagcgatct ctagaccttg
    15601 ttctcctgtg tcaggctgtg aaagaagtag ttcatcgata ttcacaccta atttttgtgc
    15661 atattgcgga tctaacgcat gctcagcatc aatgaaagcg gccgttccgc catttttttg
    15721 tacttctgca atagcgtgta gtgcaactgt tgttttacct gaactttcag gtccatatac
    15781 ttcgatgata cgtccacggg ggtaaccgcc gacacctaaa gcgacatcta acgcaagaga
    15841 gccacttgga attgtagaga tttgttggtc gattttttcg cccaatttca taatagagcc
    15901 tttaccatag ctcttttcaa tttttttcag tgcagcatct aaggctgctt tacgatcatc
    15961 tgccaatacg ataatcctcc ttatatttac aaatcagagc aacatttcat tatccttatc
    16021 atatcttttt tagtgaaaaa aagcaagaaa aaaccgaaca aatattcgca tttatcattt
    16081 tcttaaaagc tgttttctca aaagatcaaa tccttgcata accgcattat atctgatagc
    16141 agcacgatct ctggcaaagt gatatttttc cgaaaccacg ccgtctttag atgaaatagc
    16201 gatccatact gttcctgcag attgaccttc taacgtgtcc ggtccagcca ctccggtgaa
    16261 agctacactg taatctgttc ttgctatctc acgtgcttga atggccatct gctccacaca
    16321 ttctttgctg actgttccat attctgctaa tagctccgga tcaatctcca gaaaaccagc
    16381 ttttgtttgg agcgagtagg taacgaatcc gcctggaaat acttcagata caccagcaat
    16441 gtcgcctaat gcggcttgga aggcgccggc tgtcaaactt tccgctgctg ttacagtctg
    16501 cttgttttct ttcagtagtt ccactacgac ttttgctagc gaattatcat cgccgtatcc
    16561 ataaaaatat tcccctactc gttcttggat tttttcttct aatgctaaaa gagcttgatt
    16621 ccctgcctga acatcatttg ttttcactgt cagtcgtaat gtcacttcat ttggtttggc
    16681 atatggtgcg atcgttggat tgatctgtgt ctcaatcaga tcttttagct cagtgaccag
    16741 tcgcgattca ccaatcccat aaaagcgcaa aactttagaa attagttttt cttcagaagg
    16801 aaaacgttcc tctagtaaag gacgtacttg ttccacaaac attggtttca actcattagg
    16861 cggaccggga agaaggatat acgcatgtgt atctgtttga taaaaagtcc ctaaagcaag
    16921 ccctgtacga tttggtagag gaaccccgcc ttcgataatc tgggactgtt gcagattgtt
    16981 tttggtcatt tttcgatggg ttgtttcaaa atatgccaat aattttttgt atccttccgt
    17041 attttgtatg agagacttac cgaggtgagc cgcagttgct tcttttgtca ggtcatcttc
    17101 tgtcggtcct aatccgccac ataagagtat gagttcactt cttgtttcag ctagagcaat
    17161 caactcttct aaacgctgct gattatctcc tacaactgtt tgataataaa cttcgatacc
    17221 tagatcagct agctgttctg acaagaaagt ggcattcgta ttcacgactt gtcctagtaa
    17281 aagttctgtt ccgacagcta ttatttctgc tttcattact tcccgctccc ttatatagta
    17341 tacgctgtat ttttatattt ttttatttaa aaagagggca aggagctgtg acaaaaatct
    17401 tgtcacagct ccttgttcca aataaacagt gttctcctgc caaaacctcg gtcataagtc
    17461 acaatataca taaaacatga aaagctgttt tttgaaatta tcccttgtga ctcagagtta
    17521 gacggcaggc tcacaacctc tccactaaac gatacttaca ttgatccttt gaacacaccg
    17581 ctgtttttga tgaagtaatc cacacctgaa tagatagtaa agatcagaca agcataaagc
    17641 atgatttgat ctactggaat accgatcagg ctgaatggga tattattgat aaacagcaag
    17701 ataatagcaa ccatttgtgt agccgtcttg acttttcccg gccaagctgc agccatgact
    17761 tctccatgtt cgacaagtag taaacgcaag cctgtgacgg ctaattcacg acagacaatg
    17821 atcgctacta cccaaccagg ggcctttcct tggtcaacca acatgataaa agcagtcata
    17881 actagcattt tatctgccaa cgggtccgca aacttgccaa aattagtgac aaggcctcgc
    17941 gcacgggcaa tttttccatc taaccagtct gtgatactag ctaaagcaaa aataactgca
    18001 cctactagct gtgtgacagc cagtgtggtg tctcctacgt taaattgtcc ccaatctaaa
    18061 ggtaaaccta caaccaaaat gaaaatcggg atcatacaaa ttctaagtac agttaattta
    18121 tttggtaaat tcacactatc gactcctttc ttccctattc tacgtgacta tcagcgaagt
    18181 ttccaccgat attatttact tttccaaatg aatttattca aaggagtatt tttattgaaa
    18241 ttgatctttt tgaattaacg aagaaggacc gcgacacaga tttgtcacga tccttctttt
    18301 ttatacctac aagtaaacag atgcgtaaac taatttatac gatcattctg cgtattgaag
    18361 agtaaggttc acattttttg gactctgatt gttttcccct ggattaactg gtacggattc
    18421 tccattcgct ttgatcgttg cattgcttgc tgcaccaatc gtgatggtcg catttgttgc
    18481 tgtttccggt aacacggtac tctgtgtttc atttgctgcc agtgtccctt gataaacata
    18541 ggcattgtct actagcacac ctaaccatac acgattgaca gcagtaaatt caagggttac
    18601 aggcttggta gcgtctgtga tatttactgc gatggcactg ccagaatctt ctcctgtagc
    18661 tattgccatt ttcttttctt cacttgattt tgttgttgat tctactgtag aagactcttt
    18721 ggtctgcgtg ctttgctctg aggaagagga ctcctcgact gatccttcta ctttaagcga
    18781 agaagctgtg tctccgatta ttggatcagc ttgacgatct tgccaagtca tatagcccac
    18841 gacaacgaca atcgccaaag caaccaatcc taataaaatg acaggaagac ttgtccaaaa
    18901 gcgacttgga tgcgattctt cctgatgcat tgcttttcta gaagtactta ctgtttcagg
    18961 ctgcggacgt tcagggaccg atcctgtcaa tttgcttttc ccatcaaaaa catcgactaa
    19021 atggtctcca tcttctccta cagcttcagc gtattgccga ataaatgccc gcacgtaaaa
    19081 tttaccaggc aattggtcaa aatcatcatt gtcgattgct tccaaataac gtttttgaat
    19141 ttttgtaatc tgctgtaatt catctaaaga tattttttta ttcaggcgtg cttgccgtaa
    19201 tttttctccg atggtttcaa aagccactcg ttcatcttct ccagtctgtt tgattctccg
    19261 aagcatctgc tgttgcaacg aaagacgtta tgtttgggtg ctgtgacaaa agtcatgtca
    19321 cagctccttt tttccgaata aacattgttc tcctgccaac tcctcggtca caagtcacaa
    19381 tatccaataa atataagaag ctgttttttg aacttgttcc ttgtgactcg gagttaaacg
    19441 gcaggctcac gacctctttc ttaaacggtg ttcaatcagc tgttcctcgg tattagctca
    19501 aaaaccggca aaatataagg agatattttt ccgtatgttt ctctaatact caggtcaaaa
    19561 cgctgatttc acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag
    19621 tccaaccaag ttaaaaacat taagaagctg tttttacttg gctgttctta ttacttgatg
    19681 cagggcagct ttttcacaac ctcttggtac acggtgttcc agaagtagct tcttcggcaa
    19741 taagccgaaa tatcacaaaa atttgagaag caattttcgt aaattccttc ttatttcttg
    19801 aagctgacca cttctgtcac aacctctttg attacttcgt ttgattttat tttaatataa
    19861 gttgattaaa agagagcatg atttgtttta ttttctcact aatttttcca acacaactaa
    19921 gaataccata aaagctgatt ttggaggagg ttcctactac aagtctaaaa cgaaatttaa
    19981 gacttttcct cataaaatac tggtttattt tggtctcatg taaaactcgc tgaaggcctc
    20041 ttcacttata aatgcttccc cagctgccag tacgtctttc atttggatcg aatcgatgat
    20101 ttcaggaaga tcaaataaag ttcgatcgcc aaataaactt tgcgtaaatt gattggcaat
    20161 atattcgatc gaatttaatg attggaaata ttgtccaagc attttcttct tcagcaaatc
    20221 gagatttgtt tctgacactt ccggatcatc agcgaatcca agaatgattt ctttgacttt
    20281 ttctgctgct ttctctggtt cgtccgtatc tccgctgaag tccgcaaagt gaaattcacg
    20341 atccaagctg aattcaaaac caaagctgtc atcgataatt ccttgattgt acatcgctag
    20401 atagtttcga gaagtattgc ctaaaatcat ttggaacaac agattgattg ctgttttata
    20461 gcgtaacagt tctgttcctt cttgcggcaa tgtgtctaat cctttaatac caagtacaaa
    20521 tttatctcgt gtaatggctg cttccaatgc actttgtttg atgatttctt ttgtgttttc
    20581 agggaaataa cgtacaattt cttgctttgg agggaaattt tttgcttctt ggttttcccg
    20641 aataagtttc atcaatttct caggctccat cttaccaact acaaataaaa ccatattgct
    20701 tggctgataa aacgtccgat agcacgtata caagtcttga gctgtgattt tgtcgatact
    20761 ttcaaccgtg cccgcaatat cgatatgcaa tgggtgtgta ggatacaagt tatttagtat
    20821 gccaaaaaac atccgccagt tagggtcgtc ttcatacatt tggatttcct gaccgatgat
    20881 tcctttttcc ttgtttacag tttcttctgt gaagtaaggt gcttgtacaa aatcgatcag
    20941 agtcgtcaga ttcttttcta cctgatccgt cgtagaaaac agatagctgg ttttcgtaaa
    21001 acttgtgaaa gcattggcgg aagctccttg tttcccaaat aattgaaaga catcaccatc
    21061 ttctttttca aacaacttat gttctaaaaa atgggcaatt ccatctggaa ctttcacctt
    21121 ttctttttca ccatagggaa taaattcgtt atcgatcgaa ccataattcg tactgaacaa
    21181 accatatgtc ttgtgatagt catttttagg caacaaatac acggtcaaac cgtttggcag
    21241 tacttcatga taaagtgttt cattgatttg ctcatattct gttttattca tttgcatttc
    21301 ctcctgctaa aaagaagatt gcctgcaatt cgatttgctc agctacttgt tgtatctctt
    21361 tgatcgttac gctctccata cgtttcaacc attcttcatc agataatttt gtttccggca
    21421 gccagctgtc caaaaaagaa gcttcgattg ccgcttgcgg agagtctaga gaaagcagat
    21481 attggtttct cagcattgct tttgtctgag acagttccaa ctctgttatt tcaccatttc
    21541 gcaaactttc taattgctcg tgaatcagat gaagtacttg atttcggttc ttttcatcga
    21601 taccggtttg gacactcatg aatcctcgga acgtatccac gctactagag gcataataag
    21661 ctagactttc tttctctctg acattcatga aaagtttaga gtgaggaaaa ccgccaaata
    21721 aaccgttaaa gaccattagc gcaaaacgtt ctggttcatc gtaatacaca tttgtctgat
    21781 aagctaggtt cagctttgct tgaacgattg gctcacgtac ttgtcgttcc ttcacgatat
    21841 tcacttgagg ttgggtataa aacagatctg gataaactgt ttcccgcgac tcaaatggta
    21901 attgcccaac tgcttcagct gctttgtccg gatcaacatc gccgacaaca aaaatgtcga
    21961 tttgatcatt tgccatcatt tcttgatagt aggacgctag cgaactagca gtgatcttgt
    22021 ccaaatcttc gactacgccc aaccctggga tcttttgttc cgggctgttt tcaaaataaa
    22081 gttcttgcaa ggcataggaa gcaaaagttt gtttatcttc cttcaaactt tccaaatata
    22141 gacgcagatt gttcttttcc aagtcaaacg tcaactgatc aaactgatga tttttaatat
    22201 ttggataaaa taaaacctct tttaaaaaat ctaccgcttg agaaaaaagg tttggatcat
    22261 tgacatattc accattcaca aaactgatcc ctgcgtttag ccaatgtaaa tttccttttt
    22321 ttctaactga taatccaaag cttgcgccat aaagctctgc aagttgcgca cttaactttg
    22381 tctgatccga ataattcaaa ctatttgttt ccaacatgct gctgagaagt gaacgtttcg
    22441 tgatgacttc ttgttgcaga cgcgcagtaa aacggataaa aatccggatc gttttgtatt
    22501 gttttgttgg taagacatga agattcacgc cttctgccaa ttgatacacc atatttatat
    22561 gctccttttc acagttgatt gcccgcttac tcttttaatg ggtcaacctg ccttttttac
    22621 ttattcctct gcttgaaatt tataagttca ggcaattcgc cgttttttct aaatgatgtt
    22681 acactttata gtatagcata gatgtttttt atcaaaagaa acagacggct aaaagcgtat
    22741 attgtttttt tattttttat ttttcgatat actaaagaca aataaaagaa gggagggtca
    22801 ttcatgatca aagaattcaa agagttcatc atgcgtggag atgtcttgga tcttgctgtc
    22861 ggcgttgtta ttggtagtgc gtttacaagc atcgtaaacc aagtggtgca aggcttgatc
    22921 acaccactaa taggttggtt tgtcgcaatg atcacaggta cttctgattt agaaggagca
    22981 ctttcaattc ttgactggtc gccgacaaaa ggcgtcacat ttgcgtttgg taacgttatt
    23041 agtgctatca ttacatttct gatcactggt tttgtcttat tcttagttgt caaagcggca
    23101 aaccgtgcga agaatttacg ctccaaagaa gagcaagtgg aagtggaaga aactcctact
    23161 gcagaagatt atcttagtga catacgcgat cttcttgcca aacaagtggc agaagacaat
    23221 acacatccag tagagcaaac aaatctttcc gatacttcta aataataaag aatctaaaaa
    23281 aaagaaagcc gattttcagt gtcggctttc tttttttaga ttcttaactt gctacttgtt
    23341 cctttttcat gtcgatatgt ggaatatcat cttccaaata cacatctgag attgccttga
    23401 atccaaacga ctcataaaaa gcttgtaggt attcttgtgc gccgatcacg attggttttt
    23461 ctggaaaatt tttctggatc atgttaatcg tttcttgaac aatctttctt cctaaaccat
    23521 ctttacggtt gtcctgatga acaaggacac gtccaaaatg gatactctcc gcttcttcat
    23581 atactcgcgt gtaggcaagg atcttcccat cagtatcttg aaaaaacgta tgccaagctt
    23641 ctccatcaat gtcatcaact tcttgataag gacactgttg ttccacaaca aaaactgcga
    23701 ttcgttcttt gacgatcgcg taaaactctg ctgttgacaa ttcttcaaat ttctttacct
    23761 gatattccac taagcaaacg ccccctgctt ttttattctt agcataccga ttcgcacaca
    23821 aaaaagcaaa cgaaccttac gaatctcagg ctcgtttgct ttttaaactg cttcagttaa
    23881 aaattcgatc tcaactgtcc gagtaagtac atctgaatag ctgtaagata ctcgttcaaa
    23941 agaattttct tcaggatcca aatcaacaac gaatactgaa ggataggttt ctgttaaaac
    24001 gccgcggcgt tctgtttgac gctttcttcc tgtctgagca accaacatga ttgggctgcc
    24061 gatacgacct tctaaatctt tcttgattga agctaaagtt gttggcatac agttcacctc
    24121 tactatcaac tataccacat atttttaaaa ttttcaagtt taccataaaa aaaaaagaaa
    24181 tgcaataaaa ataaataaga agctcattaa gatttttcta aattattcct cattataaaa
    24241 tttcaagtgt ttttttattt tgagttttac tctcccgtaa gcgctcatta cttgcttgtg
    24301 atccattgct aaacattcag ccatgtctac aaaatcaacg ccttgcatat aataatgaaa
    24361 aattttagct tctatttttg aaaactggat ttcgcaattt tcgattgctt cttttaataa
    24421 aagatgctga attgctaaag gttcttgcaa tgtgatctct gctaaaaagt ctggtccttc
    24481 tgtctctatt ttttggtcga ttgagacttc atctttttga tcacgacgct tcagcgcgtt
    24541 ctgcttcctc aacagagaat agatttgatt gcttataacg cttttataat aacttccaaa
    24601 gctaacacct ttgctttttt cgtaagttct caatgcctga tataaagcga tccttgcttc
    24661 ttgttcccaa tcctcagaaa cataatttct taaataatag gtgtttttca aacgtagtaa
    24721 aagtggaaaa tatttttgat acagggcagc aaatgcttct tcgtcaccat tacgtgcagc
    24781 gcgcaataac tcctcagaca taaaataatt cctccataaa ttcgtttatg gaggaatctt
    24841 atcatcaatg attcatcgaa ctgattagaa cgatatgtta tttatttttt ctcattaaat
    24901 cgtccaattt ttgggacaat ttttttaact gctcttcatt ccatggagaa ttacgtcgga
    24961 aatcaataaa atgcatatct ttattttctt gggctattgt tttttccgtt ttttttatcg
    25021 tcttatataa ttcataagca gaagttcgta atgctccttg ggaaaagatc atccactgtt
    25081 ctgctaaatc gcttgtcgcc actgtcactt gtgtcaaacg gtcattcagc tgaccagcga
    25141 ttctctcgat ataactgtca gccgtttcgt cttctttcgt aaaaacaaca tccaactggt
    25201 atttcttata attttgttgg atgcccggaa ctagttgggc atcgaagaca acgatgactt
    25261 ccattccttc atatttcgca taatttgata aacgatgtaa aagagcttct cttgcatccg
    25321 ccattttgtc ctgcttttta agctggacaa gttccggcca cgaaccaatc atgttgtagc
    25381 cgtctacaat caacagctgt tttttcatgg tctactcccc ctgacgttgg gaaaaagcct
    25441 gatacataag caaaccagca gcaacactgg catttaagct ctggacgtgc ccggtcatcg
    25501 ggatcgtcag caattcatcc atttcttttt tcaatcctgg actcattcct ttgccttcat
    25561 taccaatgac taaagcaatt gatcctttgg cattccattt ttggaagttt gtgccggtca
    25621 tatctgtgcc aaatacccaa aatcctgttt ctttgagctg ttgcacagct tgtaccaaat
    25681 tcgtcacgcg tgcaacaggg acatgttcga ctgccccagt ggaagtcttt gttacgatag
    25741 gtgtgattcc aactgctcgg tgttttggta tgatcacacc atcgacgcca cttgcgtctg
    25801 ctgtccgtaa aatcgaccca aaattgtgtg gatcttcgat gctatccaaa atcaaaaaga
    25861 aggggtcttc ttttttctct tttgttcgtt caatcagttg gtcaagggat aaatattcat
    25921 aaggtgtaat cgcaagaacg actccttgat gcacgccgtg atcgctcaaa gtttctaatt
    25981 tttgtttagg aacccatttg actggcacag cttgttcacg tgcgatttct ttcagttctt
    26041 ctactttttc tcctcgtgaa tcttcttgaa gaaagagttt attccctcgc ccttgttgta
    26101 gcgcttctac tgcggcatgg tgcccaaaga cgaaattatc ttctaatgct tcttcatttt
    26161 gatcagaaac tgactgattt cgtccagttt gctttttatt tgaatatcta ttttgttttt
    26221 tgtcttcaaa tccacgtttc gattgatgtt tgtttgttct ttctttaccc atttttttca
    26281 ccaactttct gaatacacca gcggatcaat tcttccatcc gctcggtttg ttttgtcaaa
    26341 tgcagatagc ccatgagcgc ttcgaaccct gttgcgaccc gatacgtcgt aacatcagca
    26401 ttttttgcgc tcgtatggct tttagcattt cttcctctgc gataataaag ttcttcttct
    26461 tctgtcaaaa gatgttcttc cagcatttgc tgaatcaaaa aggcttgtgc attcgcagaa
    26521 acataattcg ttgccgcacg atgcagctta ttgggttttg tttctccttg atcaatcagg
    26581 tggtcacgaa catagatttc gtaaatcgca tctccaacat aagccaaggc taaaccgttc
    26641 aattgtgtgt aatctctcat gcttctcttc tccatcgggt tccttgcggt gtatcttcta
    26701 ataagatccc tttttctttc aacaaatcac gaatttcgtc gcttcggaca aaatcacgat
    26761 tgcttcttgc ttgatttcgt tcttcgatca attgttcaat ctcttcatct aataactctt
    26821 ctgctgtaaa taaaataccg aagatcgaaa gccattgcga gaataaagct tctccttgtg
    26881 ccaagacagc ggcagaaact ttcggctggt ctagataagt gttcagccac ttagcaagct
    26941 catagaccac tgtgatccca tttgctgcat taaaatcatc gtccatttct tcgatgaatc
    27001 gctgttctaa attttctaac aaagcgattt tatccgcatc atctgccaaa ttctctgagg
    27061 cagtttcttg gcggaatttc aaattgtcaa acgtattctt cagtcgttgt aaattggtgg
    27121 ctgcttcttt catcgtcgtt tcactgtagc ggatcggacg acggtattgt gtggtcgcca
    27181 tgaagaaacg caagacttgt ggatcaacgt ttttgatcat ttcatgtaca gtgatgaaat
    27241 tccctagaga tttactcatt ttttcatcgt cttcgccaat cgttacatag ccattgtgca
    27301 tccagtaatt agcgaatttt ttccctgttt tcgcttcact ttgtgcgatt tcattttcat
    27361 gatgaggaaa ttctaaatct tgcccgccac catggatatc gatcgtttct cctaaatgtt
    27421 ttgttgccat aacggaacat tcaatatgcc aaccgggacg tcctttgccc catggtgaat
    27481 cccaagaaat ctcgccaggt tttgcttgtt tccataaagc gaaatctaat gggtcttctt
    27541 tttgctgctg ttcttcccct gtacgctgac ttgctccgac ttccagttca tcgatggatt
    27601 ggtcacttaa tttcccataa ttttcaaatt tacgggtacg gtaatacaca tcacctgaag
    27661 attcataagc atagcctttt tcaaccaaca cttgaataaa tgctaaaata tctggcatat
    27721 gatccatgac tcttggatgc agtgtagcag gttcgacatt caacgctttc gtatcttctt
    27781 caaatgctcg gatgaaacga tcagcaactt ctggtgctgt aatacctaat tcgtttgctg
    27841 tacggatgat tttatcgtct acatccgtga aattcgatac gaaatcgact ttatagcctc
    27901 gatattcaaa atagcgtcga attgtatcga aagcaatggt gctacgagca tttccgatat
    27961 ggatataatt atacacagtc ggtccgcaga catacatacc gacatgtccc ttttgtatag
    28021 ggatgaattc ttcttttttt ctggtcaatg tattgtagat ttttatcatt ctgatctcct
    28081 cactttttct cctttgattc gaacgacttt tgctggtact cctaccgctg tagcgtcgtc
    28141 tggaacatct ttcaacacaa cggcacctgc accgattttg gcgttttttc cgatcgttac
    28201 cggtcctaat atctgcgcat tcgcagagag catggctcct tgtttaacag ttgggtgtct
    28261 tttacccgtt tcttttcctg tgccgcctaa tgttacgcca tggaataaaa tcacatcgtc
    28321 ctcgatttct gcggtttcgc caatgacgat tcccattcca tggtcgatga acacgccagt
    28381 tccaatcgtt gctcctggat ggatctcaac accagttagg aaacgccaga attgagcatt
    28441 gatcttcgcc aataaaaaga gccggtgctg atataaaaaa tgagagaaac gatgccaaaa
    28501 caaggcatga aaaccagggt atgtcaaaac gatctcagct gtcgaacgtg cagccggatc
    28561 gtttctcttt gctgcatcaa tcgctcgggt aaaccaactc atgactttcc actcctttcg
    28621 taagaatttt atgtaggaaa tcatcttctt ataaagaaaa gaagcgcctt ttaaataagt
    28681 ttccttatca aaagacgctt tccgcgtggt tccacttttc ttcataatca atgattatct
    28741 ctgacagcta acgcctgtta tacgttcttg gctactttgt ttcctataaa acagtttttc
    28801 ctgttttctg tgtttcgcca agaccactcc cagaggcatt tcggcaacca ttcattgata
    28861 ccttgcacca cccggtatct ctcttgtaat gatcagctgc ttactttttt ctgttcatcg
    28921 tgtttagttc agtacttgat ttaaatgtgc taatgatttt tcacgaccaa gcaattcgat
    28981 cgtttcaggc aattctggac catgcatttg tcctgataca gctacacgga ttggcatgaa
    29041 aagatttttt ccttttacgc cagtttcttt ttgaacagct ttgatggcag ctttgacagt
    29101 tggagcatct actacttcca tttgttctag ctgtgctttg aatgcgtgga gaacagtagg
    29161 aactgtttca ccagcaagta cttcttttgc tgcttcattc aatacaggat gttcattgaa
    29221 aaatagcgta gacagatcaa cgatttctgc agcatagctc atttgaggtt gatacaggct
    29281 aacgatcttc ttcacccatt cgattgtctt ttcatctggg ttttctgcta cacgtccatc
    29341 agcgattaga taaggcagac acatgtcagt aaatgcatct agatccattg ctttgatgta
    29401 atggttgttt acccattgca gttttttcgc atcgaaagct gcgggtgatt tacttaggcg
    29461 ttttggatcg aacattttga tcaattcttg gtgttcgaag atttcgtctt cgcccacagg
    29521 tgaccagccc aataatgcaa taaagttgaa cattgcatct ggtagatagc ctaattcgcg
    29581 atattgttcg ataaattgca agattgattc gtcacgtttg cttagttttt tacctgtttc
    29641 actgttgatg atcaatgtca tatgtccgaa ggttggtggt gtccagccaa atgcttcata
    29701 gatcatcagt tgttttggtg tattggcaat atggtcatct ccacgtaaaa cgtgagtgat
    29761 tttcatcaaa tgatcatcga ccgcaacagc aaaattataa gttggcatac catcgcgttt
    29821 ttggatgaca aagtcgccac cgatattatc ggattcgaac gaaatagcgc ctttcaccat
    29881 atctgtaaat gcgtattctg tattgcgagg cacacggaaa cgtacaacag attctagacc
    29941 ttgtgcttct ttttctgctt gttcttcagg tgtcaagttc gcacatgttc ctgcgtaatg
    30001 aggcatttct cctcttgcgc gttgtgcttc ccgttctgct tctaattctt cttctgtgca
    30061 atagcatttg tatgctcgat tgctagcaag cagctgatca atcagcggtt gatagatttc
    30121 tttacgttca gattgacggt aagggccgta ttctccagga ttttcaggag attcatccca
    30181 attcattccc aaccaagcaa ggttttccaa ctggcttttt tcgccatctt cgatattacg
    30241 tttttggtca gtatcctcaa tacgaataat aaagtctcca tcattgtggc gggcaaacaa
    30301 ataattgaat aatgcagttc ttgcatttcc aatatgcagg tgcccagttg gacttggtgc
    30361 atagcgtacg cgtactttcg tcattttctt cctcttcttt caaactagac tttgactaac
    30421 agcaaaaatt ccgtcagtta cggcttctca aatatcattt atttaaagcc actcagcaca
    30481 aattttacct tttctaaggt aaatagtaaa gccaactggt tgaatttcct taccagttgg
    30541 ctgaatttct tttttatttt gattgtttag tctttttctc tgtctctttt ttattgtcga
    30601 ttcctcgtcc tgcatgtgca ggtttagcaa agatcatgcg tccagcagct gtttgaaggg
    30661 cacttgttac aacgacttcg atacgttcat tcatgtaatg ctgtccatct tctacgacaa
    30721 ccatcgtgcc gtcatcaagg taggctactc cttgttggcg ttctgtcccg gcttttacta
    30781 ctagaacatt catcgtttca ccagggatga cgactggttt tacggcattt gccaacgcat
    30841 tgatattaag aacaggtaca ttttggaatt cagagacttt attcaagtta tagtcattgg
    30901 ttacaatgac accatctaaa agcttagcca atttgatcaa tttgctgtct acttcactga
    30961 tatcttcaaa gtctccatcg tacatttcga cagatatacc ctcttctttt tgcaaggcat
    31021 ttaggatatc taatccgcga cgaccacgca cacgtttcaa actatcccct gagtccgcga
    31081 tgtattgtag ttcgtaaaga acaaaattag ggatcatcaa ggttccttct aagaatccag
    31141 tctttgtaat atcataaatc cgaccgtcaa tgatcacgct tgtatccaag attttatatt
    31201 tatggaaatt ttctcctgat tttcgttcta acagttgact ctcaacttga ctagctgttt
    31261 cttgctcgat ttttttgctt cgtgaaccaa aaactttctt ccattcttcg attcgtgtcg
    31321 ttcccattct caatcccaga tagcctaaga tgatcatcag taaaatcggc aatacactat
    31381 tgacgaatgg aatttccata ttgtaaagag gggtcgagat cacaacacct aaagtcaatc
    31441 cgatgatcgc accgatcgca ccgaagagaa gataagtcaa gctgacctca cttagttttt
    31501 gttctacttg tttgataata gctgagatat gtttggccaa cagcaaagat aataaaaaga
    31561 aaataagtgc gcctaacagg ctgttggtaa atgtattatt cagccatccg tttgactgtt
    31621 gaccgattgc ctgccaagct gagggtaaaa atgaaatacc caagcttgcg ccgacaatga
    31681 tcatcagcaa agtaatgaca cgtttttgca tcgtttttcc tccgtttcga caatcattta
    31741 ttagttagcg gaacacacgc ttcaacgttt cagccaaagt tgctactcca actatctcga
    31801 ttccttttgg cgcttcccag ttacctaagt tgtttttagg taggtatact tttgtgaagc
    31861 cgagtttttg gacttctttg actcgttgtt caatatgact gacacgtctg atttctccag
    31921 tcagtccaat ttcaccgatg aaacattccg ttggctgtgt tcctttttct ttatagcttg
    31981 aagcaatact gacagcgata gctaaatcaa tcgctggttc atttaatttt accccacctg
    32041 cagcttttaa gtaagcatct tgattctgaa gaagcaaccc agctcttttt tccagcactg
    32101 ccatgatcag agatacccga ttgaaatcaa gacctgttgt tgttcgtttg gcatttccga
    32161 acatagtagg tgtgactaat gcttggatct ctaccaaaat tggacgtgtc ccttccattg
    32221 ccacaacgat agcagagcca gttgcaccgt ctaggcgttc ttctaagaaa atctgtgaag
    32281 gattagcgac ttcttccaat ccatgctcat gcatttcaaa aatcccaatc tcattcgttg
    32341 agccaaatcg gttttttact gctcgcaaaa tccgaaaact atgatgttgt tcaccttcaa
    32401 aatataacac cgtatccacc atatgttcta acattcttgg gccggcgatc gagccttctt
    32461 tggtaacatg accgacgata aaaatggcta tcccatttgt tttagcggtt ttgagtagtt
    32521 ctgcagtggt ttctcttact tggctgacac ttcctgccac acttgtgatg tctggctgcg
    32581 tcatcgtttg gatggaatca atgataacat aatcagggga gatatgttcg atggcacgac
    32641 tgatttcgtt catatctgtt tcagcgtata gataaaattc tgtgttgata cttccgaggc
    32701 gttccgcgcg taattttatc tgctctgcgc tttcttcgcc ggatacatat aagacttttc
    32761 cgcctatggc tgctaattgc tgagaaactt gtaacagcaa agtggatttc ccgatacctg
    32821 gatcgccacc aatcaagacc atagaccctg gcaccacacc tccgcctaac acacggttca
    32881 gttcttctaa ttttgttttg atccgaggtt cttttttagg gacgacgtct gcgatttttg
    32941 ttggttttgc tttttctcct gtcaaacttg tccgagttct gcggtccgtt gtatcttgtt
    33001 cgatttcttc gaccatcgta ttccattttc cgcaatttgg gcagcgtccc aagaacttag
    33061 gcgacacata cccgcaattt tggcaaacaa attgtacttt tgattttttt gccattgtac
    33121 cgcctccttt tatcctaatc atgagacatt aaatcaagct taagtatctt taacgtttta
    33181 tttactggaa gaaccaaatc cgccttgacg ttgagcagtc gtttgatctt gatctgcttt
    33241 taaaaacggc aaaaagatcc cttggccgat acgctcgcct tttttgatca ctacatcctt
    33301 gaaaccaaag ttggtgaatt ggaacatgat atggccttca tttgcctcat tattgtaata
    33361 atcactgtca atcacaccta ctccgtttgt caacactaaa aaccgcttca acggattgct
    33421 tgaacggtta gcaagctgca aaaattcatc ttctcccatg taagctttga ttccagtagg
    33481 cacgagggtt ggtttcaatt gtttttcatt ttctgataca tcttttttca atccatctgc
    33541 gactgtttcc aacccctttt tccagcctgc tttccagata cttggaatca cgacctcttc
    33601 tgctgcttca aaatcatagc cagcagcatg atgtgtagcg cgctgcggta gattgatatt
    33661 ttgatcttta tatttagaaa taacttcaaa tcctcttttt ctcaccggtt ttcctcccta
    33721 aataaactac tcctattcta acatatccga ggctgtaaaa gactttcttt acaaattctt
    33781 caagaaattc tgctatctta aagtaaacca ttaattcgtt ctttttccaa acgactgcaa
    33841 gttgtacaaa aaaactattt tttacatgac tttactcgtc aaaacaagta gaatagaacg
    33901 aaattaatga gtggatattt gattgaggtg tcacatgcgt aaagaaatta aaaatgcaaa
    33961 acgaattatt gtcaaagtag gaacaagtac attgatttat cccaatggaa atatcaatct
    34021 tgcggcaatc gatcagttag cttttgtcct ttctgatctt agaaatcggg gaaaggaagt
    34081 gatcttagtc tcatcaggag ctgtaggcgt tggtatgcat aaattacatt tgtcagaacg
    34141 tccgactttg attcctgaac agcaagctgc ggcggctgtt ggtcaagccg aactgatgaa
    34201 tatctataat caaagattca gtgtatataa tcaacagacg gcacagatcc tcgtgacaag
    34261 agacgtggta gattatcctg aaagtcgtaa aaatgtgagg aatacattgg aacagctttt
    34321 gattatggac acgatcccta tcgtgaacga aaacgatact gtagcagtgg atgaattaga
    34381 tcatctgaca aaatttggcg ataatgatca gctgtcagcc atcatggcaa aacttgtcgc
    34441 ggcagacctt ttgatcgtcc tttctgatat cgacgggttt tattcagata atccgactac
    34501 aaatcccgag gctcagatgt atcgctatat cgataaaatc gatgaaaaga ttctggcaca
    34561 agcaggtggt gctggtagca catttggaac aggcggtatg cagagtaaat taaaagccgc
    34621 ttctaggata tttgaagcaa acagcgcaat gattttagca aatggaaaaa atccggcaat
    34681 tatttttgat attcttgcag gaaaagaagt aggtacttta tttaaggagg acttgaaatg
    34741 actgacttga tgcaattagg acaacaggca aaagatagcg cgcaacaact agcacttatg
    34801 cctacaaacc gcaaaaatga gctacttgtt caaatggcac aaaccatcaa aaagaatcaa
    34861 caagcgatca ttgaagcaaa tcataaagac ttagaaaaag cagtcgaaaa caatatcagt
    34921 gaaacgatgc aagaccgctt gcagctgaca cctgagcgaa tcaatgccat ggctacagag
    34981 atcgaaaaaa ttgcttcgtt agacgatcct attggaaaag tagatgagat gtggacgaac
    35041 accgacggat tgagaatcgg caaaaagcgc gtgccactag gcgtgatcgg catcatttac
    35101 gaatctcgtc caaatgtcac gacagatgca gctagtctag cattcaaatc agggaatgcc
    35161 gttatccttc gcggtggcaa agaagcattc ttctcgaatc aattattagt ccaattattg
    35221 cagcaagtac tggtcagtgc tggcgaatct ccttatgcca tccagtttgt cgatgatact
    35281 tctcatgaaa ctgcaggaca gttgatgcga ttgacagaat acttagatgt gctgattcct
    35341 cgaggcggag caaaattaat ccaacgagtc aaagaacagg caacggtacc tatcattgaa
    35401 acaggtacag gaaataatca cgtatatatc gataaagaag cacagttatc tatggcagtg
    35461 aacatcatcg tcaatgcaaa agcttcccgt ccatctgtct gtaacgcggc agaaacattg
    35521 ctgatccatt ctgagatcgc accttttttc ttgccagcta ttgaaaaaga actggtagaa
    35581 cacggggtct ctttacgtgc ggatgctcgt gcgttagaat atctagagac ggctgcttta
    35641 gcagaagaca cagattggga tactgaatat ctagattata tccttgctgt caaagtggtc
    35701 gattctttgg atgaagcaat tgcacatatc aatcggcata atactaaaca ttcagaaacg
    35761 atcgtgacag atagttatgc ggctagtcaa cgtttcttaa atgaagtgga cgcggcggct
    35821 gtctatgtca atgcctccac tcgttttaca gacggatcgg tatttggttt tggtgcagag
    35881 attggtatct ctactcaaaa gttgcatgcc cgtggtccaa tggggttaaa tgaattaact
    35941 tccacaaaat atatcattta tggcgatgga caaatccgcg aataatgttt cgtgtgaaac
    36001 attattcccg ccttttgcct tttttctgta aaggttctat ccaactagaa aggtgtcaat
    36061 atgtttgaaa ttttaatgat tttttttatt taccttattt ctctgaatat cgctgctttt
    36121 ctgggagtcg gtattttatc ccttttcttt caattcaaaa aacgaagcat agggagtcaa
    36181 cgggaaaaat ggtcgcagta ttttgacaaa atcgggcaaa aagggctggt tacacgcttg
    36241 cacattagtt acatggttgc attatgctta ttagcgacaa tcaattacta ttcttttttc
    36301 gatcattcaa tcgcttatac gattacgcta ctgattgctg ggatttttca cttatcttat
    36361 aaatatcagc tgaataaaaa ccacttgaat cggactttcc gctaaaaaat atccgtacga
    36421 taagtagagt gccttgaaga tcggcaaaaa actgccggac tttttagagg ctgtgacaaa
    36481 gattttgtca cagcctcttt ttccatataa acggcgttcg cctaccagct gcctggtaaa
    36541 tctgtttcct cttcgttaag aagcgaaaat ccgtcaaatg tttcgccatc ataataacga
    36601 agcagctcat ccatggacaa gttcatcgtt ttttcttttt cttcttccgc tgattcagcc
    36661 agtagctgtt cccgatacac ctgatacata ccagctattt tttgctgcat cgcttttcta
    36721 gtttcacctt ccgcattgat tattggcaat tctttgatat agccacgata aatcaaccga
    36781 tcatctgaaa attcaatggg taattcaacg agtgtcaacc cgacttcctg aaaatattct
    36841 tccagcatct attatccccc cattcttttt cttaaatgaa ttttagctta cctactgaaa
    36901 attttccagt ttcattattt tgagaaaaaa agggaatgtg acaaaaatcg tgtcacatcc
    36961 cctttttccg aataaatggt gttcttctgc caactcctca atcataagtc acaatattca
    37021 acaaacatga gaaactgttt tttgaaattg ttccttgtga ttcagagtta aacgacagtc
    37081 tcacaacctc ttggtaaacg gcgttcaatc agctgaccct cggtattagc tcaaaaaacg
    37141 gtaaaatatg agaagctatt tttccgtttt gtttctctaa tacttaggat aaaacgctga
    37201 ttttacaacc tctgttaaac ggtgttcaat cagctgtcct tcggtattag ctcaaaaaac
    37261 ggcaaaatat gaggaactat ttttccgttt gtttctctaa tactcaggac aaaacgctga
    37321 ttttacaacc tcctgataaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc
    37381 aaacgaaaaa tatgagaagc tatttttgtt tggctgttct tatgacttga tgcaggacag
    37441 ctttttcaca acctctgatt tcttattctt cttcatttgt agcaaaataa ttacggtgat
    37501 aaatgataca acatacactg ataagaacaa tggcacctgc aaaataaaac gcatagcttt
    37561 caccaatatg ttcaccaatc acacctgcaa taaatggtgc taaagctcca ccgaaaaagc
    37621 ggacaaaact atagctggac gaagcaacgt ttcgttgcag acctggtatt tccatagcaa
    37681 tcgtcgtcaa cagtgtattg ataattcctt ggaaaaatcc tgctaacaca atgctgatcg
    37741 gtacaaggac tggcatagaa gttccagctc ctaataacag caaacaaata atgaataaga
    37801 caaatgctgc taaaattgtc cgataagtcg taaacttccg ctcaagagcc ggtgcaacga
    37861 aaatcgaagc aatagccaga agcacacccc agccaaaaaa tacaaaaccg acttctaatt
    37921 cagaatagcc aatcaataaa aatggcgcat acgcaagtaa agtaaagaat ccgaaattat
    37981 ataacaatgc gattagtcca actgaacgaa gtttatgatt gcttaacgct ttgacacctt
    38041 cccaaaggcc tacttttctc tttggtttct cagtttcagg taaaagtacc gctagggcta
    38101 taaaaccgca aaacatcaaa aatgctaccc caaaaaatgg ataacgccat gaaccactgc
    38161 ccaagacccc accgactaga gggccaacag ccattccgca gccaagtgct gcttcataca
    38221 tcatgattgc tttttctgtg ttaccaacta aaatactgac gatcgttgct aaagcagtag
    38281 agacaaataa agcattcccg acaccccagc cggcacgtaa cccaatcagc attccgatat
    38341 tcccagataa tccgccaaga ccagcgaata caacgatgat aaacaatcct gtcatcaatg
    38401 tttttttcaa accgatccga ctagataaga aacctgtaaa tagcatcacc acaccagtga
    38461 ctaacatgta actagtaaat aacagggtcg tttcagctgg cgtagcgttt aactgtgcag
    38521 caatcgtttt taagattggg tctactagac ccactcccat aaaagcgatc atacaggtag
    38581 ctgctacaac tagcgctgaa cttttcccct ttgtttcttc atttgtttgt tcttgtatat
    38641 ctatttgatt ctccaatatt tccatccctt tctctctatt agcatcatac atgatttatt
    38701 ttacatgtca atatgcacat gtaaaacgtg gaaactttac tgtgatataa taaagaaaat
    38761 aagacgagag gtaggatttt tatgaatact ttagcctata tattattatg cgtgctttta
    38821 tccaaaccac gttctgggta tgaattgaag cagctgatca caattttttg ggaagcccat
    38881 cacagtcaga tctatacaac gcttgccaag ttagaccagc agggttatgt gaccgttttg
    38941 gacaaagacg agcactctca aaaaaagatc taccacttga ctgatgaagg acgtgctctt
    39001 gtagaggaat ggatcaaaga ggaaactccc gctcctacgc aaaaagatga atttctagca
    39061 aaaatctatg ctttttcgac gctcgataaa acaacagcta ctggactgct attcactaga
    39121 gaacaacagc tgaacaaaat catgcaaaaa aatcaaaaaa aattgaatga cctctcgcta
    39181 gacaagaaac aagaatttgg acgttatgtt gtgatccagc gcagaattat gctttgtcag
    39241 caggaactcc aatggtgtca gcaagtaaga gaacaaatgc agcagttttt tgagtaaggg
    39301 ataaacagaa cctttctaga aatatctatt gaagagcaaa ttatcttcaa taaaagagca
    39361 atacagttgg aaaagaaaaa gcgatccgaa cgacaggcca aaacctatcg ttcggatcac
    39421 tatttttcta gaactgcatt caagagacta tgacaaaaag catgccaagg ccttttttca
    39481 aataacagtg ttccatcatt cattactttg aattgctttt agcacacttc ttgaaagcgg
    39541 gccaacgatg atcaactgca acggtaaagc ggcaatcaca ttcattttcc aagcattcat
    39601 ataagcagag aataattgat caggtattcc ttgttccatg atcacaccga ataaagacat
    39661 acatgtcacc attcctaaaa ccatcaagca tgaaatagtt aagatcattt ggaccttgct
    39721 ttctggattt actggtagtt taaaggcaat ttttttcgcc agcacaccga tgatgaagac
    39781 atccaaaatg aaagccacca cgaatccagg cactagacca atcaacaaac ttgataaatg
    39841 aagatcttga tgcagccata aattatacgc gctcatcccc aagaccatca aaaaacacat
    39901 cattgttgta aaaattattc cttctttttt atttgtaggc aatctgtacc actcctttaa
    39961 atttgtcacg aaaatcatcg tagcacattt aaaaatttct cgtaagtact ttttttattt
    40021 tctgcccttt tatttcataa acttcatcag ccgtacgctc gacaaagtaa gaatcatgag
    40081 aagtaaacaa aatcgttccc ggatatattt gcattagctt ctctaaagct tcgatcgttg
    40141 ccatatcaat aaaattagtc ggttcatcta acagcaacac attacttggc ttagtaaaaa
    40201 gcaaagcaat cgtcagacgg gtcgcttctc ccccacttaa tgtacataac ggtttcgtca
    40261 aagtttcctg agcaaaccct aagtgattca gcaaactacg gacaagcgat tctgaaaact
    40321 ctgtttgtct catgacaaaa cgaatgatgg attcttcaga tgtcatttga taaccctttt
    40381 gctgatatac ttgaaagctt actttcggag agagaaggat tccttctcct tgtttgcgga
    40441 tatgttctaa taaggttgtc ttacctgaac cattttcacc gacaatcgcg atttttttac
    40501 ctaaagaaaa ctgaaaatca ccatttacca gtaatgttct cccacttctt tccaattgga
    40561 cattttgtcc catgatggga tagcggctgt ggagttcaag agattttggt actggaaaac
    40621 ggatttgttt catctgctgc ggcttggttg tttctcctat tttttggagt cttctctcta
    40681 atgttttcgc attcttttgg atggcttttt gtacactgtc tttttgttta gaggaagaca
    40741 accgatccag tctgatttgt tgctttttct ttttactgga cactttttcc aacttttccg
    40801 cttgtttttt cttttcttgg atcgattttt ccaatcgttt tttctctttc atgatcgact
    40861 ctgcttctcg tagctgagtc tttttctcca attccttttg agtgagatag gcactataat
    40921 tccccgaaaa ctctcggatt tctccgtctt ttacttccca gattttcgat gctagctcat
    40981 ctagaaaata gcgatcatga ctaacaaaaa ccagcgtacc ataataataa cgaagctctt
    41041 caatcagtcg atcaatactt tttctatcta gatgggttgt tggctcgtct agaagcatcc
    41101 cttgttcata agaagaaaga agacgtatca actcgatctt tcgttcttcc cctccgctcc
    41161 atttccgctc tgaagtttct tccagtctta actggaaatg actggctaat tccaaattta
    41221 ggtcctttgc ttcagcgaga taggttaatt gaggcaggta gttgaaggtg atctctgttt
    41281 ggattttccc atgatcggga aaaagttcac cggctattag tttgagcaaa gtcgatttac
    41341 cgacaccatt ttctcctatg atccctatac gatcttgctg atagacacga agagaatcaa
    41401 tagatagttc tagctggttg ccaaagctga cttgtagatt tgttatattt actgctaaat
    41461 tttccatatg ttttacactc ctatttcatg taaaacaaca attatcgata ctaaaaaaga
    41521 gcagaaaaat ctgtttgcaa aaaaactgca tcacatcttt tcctactctg aacgaaataa
    41581 ctatgactaa atcaccaaat ctgtcatagc tttcacctat tctactgtcg ttcgaaaaaa
    41641 tggacatact ccatccatga aattgttgtt tatacatgtc ttttgaattt atttagttgt
    41701 tattcaaaag ttcgaaacgt aatttcatcg atgcagtcat ttttccgacc tcctttttta
    41761 ttaggttacc acacacgatt caaaaaaagc aatagctact gaatagtaac tgaaaacctt
    41821 ttattcgaac atagtgatct tttctggtgc taaacgaaca gactcgtaac cttcttctac
    41881 cgcttttcct attgataaaa taataactgg aacataacgg tctttatcca aaccaaatgc
    41941 ctctgccaac tgatctgctt cgaatccacc aattggattt gtttcatagc catgatcacg
    42001 cgcaactaac atgaattgca tagccgctaa gctagaatcg actttgacta catcgttcat
    42061 ttcttctctt gaaaaactct tgtaataagg aatgatcgca gctaattgtt gatctctgac
    42121 ttcttttggc atcttccctg attcgtaagc ttggttgtag atttcttcgc catattcgta
    42181 acattgcata tcgccaaaaa tcatgaccat tgcagaagaa gtatcatttt gtctcgtatt
    42241 gaaacggatc aaaggacgca agatttcttt tgcttcctcg ctttcagcga ccacaaagcg
    42301 ccatggtttc atattgacag aagaaggtgc agtcgtcgct tctttgatca tttccagcat
    42361 ttcttcgtgt gggattttgt atgtttcatc atatacgcga acggatcttc taccagtcat
    42421 aattgttgaa aaatcatttt tttgttccat tattattaac ctctttcgtt tctgttgtta
    42481 tattgcaaag ttgggtattc aatttatcta aaagtgttag aaattgtttc atttctacat
    42541 cgctgaaagc cggggatagc acggaacctg tatctttatg gtccatttca caatgcttga
    42601 ttttcttctg ggctttttct gttagcgcta catacacttc ccggttgttc tgaggatttc
    42661 tctgacgcga aacatagcca tttgcttcca ataccttcag atgacgagta actgctcctt
    42721 gatcaatctg caacttttct tgcaaactgc tttgtagcaa tggattttct tctttcagtc
    42781 tcatcatgat ctcgtatcta gtaagactta taccgatatt ttcttcaaag atctggacga
    42841 ttttttgatc ggctaatttg atttgatata aaaggtgact aatctcttct aaagacataa
    42901 tttcctcctt tctaataatt gactcgtcaa cgattgactt gtcaataata gaataaaaaa
    42961 agaaaaaagt caaagaatca gctg
//
LOCUS       DAYYME010000005        42372 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000005 DAYYME010000000
VERSION     DAYYME010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.005"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(58..1161)
                     /locus_tag="ACHBFW_000201"
     CDS             complement(58..1161)
                     /locus_tag="ACHBFW_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFM9316551.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(1383..1853)
                     /locus_tag="ACHBFW_000202"
     CDS             complement(1383..1853)
                     /locus_tag="ACHBFW_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFM9316552.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            2186..3826
                     /locus_tag="ACHBFW_000203"
     CDS             2186..3826
                     /locus_tag="ACHBFW_000203"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFM9316553.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            3980..5302
                     /locus_tag="ACHBFW_000204"
     CDS             3980..5302
                     /locus_tag="ACHBFW_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFM9316554.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(5364..5795)
                     /locus_tag="ACHBFW_000205"
     CDS             complement(5364..5795)
                     /locus_tag="ACHBFW_000205"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9316555.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(5859..6695)
                     /locus_tag="ACHBFW_000206"
     CDS             complement(5859..6695)
                     /locus_tag="ACHBFW_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFM9316556.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(7437..8063)
                     /locus_tag="ACHBFW_000207"
     CDS             complement(7437..8063)
                     /locus_tag="ACHBFW_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316557.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(8358..8693)
                     /locus_tag="ACHBFW_000208"
     CDS             complement(8358..8693)
                     /locus_tag="ACHBFW_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM9316558.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(8680..8964)
                     /locus_tag="ACHBFW_000209"
     CDS             complement(8680..8964)
                     /locus_tag="ACHBFW_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM9316559.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(9020..10543)
                     /locus_tag="ACHBFW_000210"
     CDS             complement(9020..10543)
                     /locus_tag="ACHBFW_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM9316560.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(10536..11711)
                     /locus_tag="ACHBFW_000211"
     CDS             complement(10536..11711)
                     /locus_tag="ACHBFW_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM9316561.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(11715..11900)
                     /locus_tag="ACHBFW_000212"
     CDS             complement(11715..11900)
                     /locus_tag="ACHBFW_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316562.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(11866..13560)
                     /locus_tag="ACHBFW_000213"
     CDS             complement(11866..13560)
                     /locus_tag="ACHBFW_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM9316563.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(13557..14030)
                     /locus_tag="ACHBFW_000214"
     CDS             complement(13557..14030)
                     /locus_tag="ACHBFW_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM9316564.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(14099..14254)
                     /locus_tag="ACHBFW_000215"
     CDS             complement(14099..14254)
                     /locus_tag="ACHBFW_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFM9316565.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(14388..14768)
                     /locus_tag="ACHBFW_000216"
     CDS             complement(14388..14768)
                     /locus_tag="ACHBFW_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM9316566.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(14772..14987)
                     /locus_tag="ACHBFW_000217"
     CDS             complement(14772..14987)
                     /locus_tag="ACHBFW_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316567.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(14990..15397)
                     /locus_tag="ACHBFW_000218"
     CDS             complement(14990..15397)
                     /locus_tag="ACHBFW_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFM9316568.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(15663..17138)
                     /locus_tag="ACHBFW_000219"
     CDS             complement(15663..17138)
                     /locus_tag="ACHBFW_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFM9316569.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(17128..17976)
                     /locus_tag="ACHBFW_000220"
     CDS             complement(17128..17976)
                     /locus_tag="ACHBFW_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFM9316570.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(18013..18375)
                     /locus_tag="ACHBFW_000221"
     CDS             complement(18013..18375)
                     /locus_tag="ACHBFW_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316571.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(18376..18534)
                     /locus_tag="ACHBFW_000222"
     CDS             complement(18376..18534)
                     /locus_tag="ACHBFW_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316572.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(18675..19299)
                     /locus_tag="ACHBFW_000223"
                     /pseudo
     CDS             complement(18675..19299)
                     /locus_tag="ACHBFW_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(19296..19607)
                     /locus_tag="ACHBFW_000224"
     CDS             complement(19296..19607)
                     /locus_tag="ACHBFW_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316573.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(19651..19944)
                     /locus_tag="ACHBFW_000225"
     CDS             complement(19651..19944)
                     /locus_tag="ACHBFW_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316574.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            20138..20785
                     /locus_tag="ACHBFW_000226"
     CDS             20138..20785
                     /locus_tag="ACHBFW_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9316575.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            20846..21991
                     /locus_tag="ACHBFW_000227"
     CDS             20846..21991
                     /locus_tag="ACHBFW_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9316576.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(22072..22377)
                     /locus_tag="ACHBFW_000228"
     CDS             complement(22072..22377)
                     /locus_tag="ACHBFW_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFM9316577.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(22402..22833)
                     /gene="ruvX"
                     /locus_tag="ACHBFW_000229"
     CDS             complement(22402..22833)
                     /gene="ruvX"
                     /locus_tag="ACHBFW_000229"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFM9316578.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(22830..23096)
                     /locus_tag="ACHBFW_000230"
     CDS             complement(22830..23096)
                     /locus_tag="ACHBFW_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFM9316579.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(23351..24076)
                     /locus_tag="ACHBFW_000231"
     CDS             complement(23351..24076)
                     /locus_tag="ACHBFW_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM9316580.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(24437..24507)
                     /locus_tag="ACHBFW_000232"
     tRNA            complement(24437..24507)
                     /locus_tag="ACHBFW_000232"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24473..24475),aa:Gly,seq:ccc)
     gene            24624..25376
                     /locus_tag="ACHBFW_000233"
     CDS             24624..25376
                     /locus_tag="ACHBFW_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM9316581.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            25392..25871
                     /locus_tag="ACHBFW_000234"
     CDS             25392..25871
                     /locus_tag="ACHBFW_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM9316582.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(26122..27702)
                     /locus_tag="ACHBFW_000235"
     CDS             complement(26122..27702)
                     /locus_tag="ACHBFW_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFM9316583.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKKPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(27695..28438)
                     /locus_tag="ACHBFW_000236"
     CDS             complement(27695..28438)
                     /locus_tag="ACHBFW_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9316584.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(28614..29390)
                     /locus_tag="ACHBFW_000237"
     CDS             complement(28614..29390)
                     /locus_tag="ACHBFW_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM9316585.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(29638..30474)
                     /locus_tag="ACHBFW_000238"
     CDS             complement(29638..30474)
                     /locus_tag="ACHBFW_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFM9316586.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(30485..31414)
                     /locus_tag="ACHBFW_000239"
     CDS             complement(30485..31414)
                     /locus_tag="ACHBFW_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFM9316587.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            31606..31959
                     /locus_tag="ACHBFW_000240"
     CDS             31606..31959
                     /locus_tag="ACHBFW_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM9316588.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(32033..32689)
                     /locus_tag="ACHBFW_000241"
     CDS             complement(32033..32689)
                     /locus_tag="ACHBFW_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM9316589.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            32915..34849
                     /locus_tag="ACHBFW_000242"
     CDS             32915..34849
                     /locus_tag="ACHBFW_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9316590.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(34935..36158)
                     /locus_tag="ACHBFW_000243"
     CDS             complement(34935..36158)
                     /locus_tag="ACHBFW_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM9316591.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(36152..38023)
                     /locus_tag="ACHBFW_000244"
     CDS             complement(36152..38023)
                     /locus_tag="ACHBFW_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFM9316592.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(38044..39162)
                     /locus_tag="ACHBFW_000245"
     CDS             complement(38044..39162)
                     /locus_tag="ACHBFW_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM9316593.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(39245..40078)
                     /locus_tag="ACHBFW_000246"
     CDS             complement(39245..40078)
                     /locus_tag="ACHBFW_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9316594.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(40071..40988)
                     /locus_tag="ACHBFW_000247"
     CDS             complement(40071..40988)
                     /locus_tag="ACHBFW_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9316595.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(41215..42339)
                     /locus_tag="ACHBFW_000248"
     CDS             complement(41215..42339)
                     /locus_tag="ACHBFW_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9316596.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT        12693 a         8582 c         7057 g        14040 t
ORIGIN      
        1 taaaaatagg agtacggcat catctgccgc actcctattt ttttcgtcat ttgtttctta
       61 aataaaacga ttctcacgca attttatttt tttgatatct tctactgttt gtgttccagc
      121 taattgcatg accatttcca attctgtttt aaagaaatca aacacttgat ggactcctgt
      181 acttcctcct aaagacaatc cataaataac gggtctgcca atagcaacaa gatctgcacc
      241 tgatgcgatt gctttgaaaa catgctgtcc gcgtcgtaca ccgctgtcaa acacgattgg
      301 tacacgttta tctactgctt ctgccacata ttgcaacgaa tcaaacgctg ctggaccacc
      361 atctagttga cggccaccat gattagatac ccagattccg ccagcaccag attctaatga
      421 acgatagaca tcttcttctg actggacacc tttcacatag actggcaaat cagaatgagc
      481 agcaataaat tctacatctt ttggactaag cttctgtttg gaagatttgt acacagcatc
      541 catcgtttgg cctactcctg attgataagc ctgaacgatc ggcattggca gtgggaaggt
      601 aaacccattt ctgcggtctg tttcacggtt tccgccaact gttgcatccg ctgtcaatac
      661 gattgctttt gctccattac gcttggccac ttcaagaata tccaaattga tcccatcatc
      721 tttgctcata taaaattgaa accattgcgg tgctttctcc ccgcctgctt ctcttatctc
      781 ttctaatgta caagaagcgt aagaacttgc tgtatagatc gtcccaaaat ctgctacacc
      841 ttttgcagaa gctttctccg ccttcacatg agctaagcca tgagcagcaa ctggtgccat
      901 gatgattggt gcagtcagcg tttctttgtc aaaatgtgta gttgtatcgg gtaattctac
      961 atcttttaat acatgaggaa taatcagctg atgattgaat gcccgttcat tttcttgata
     1021 agtaaataag tcgccagcac cactgctgat gtaaccataa ccaccagctg gaatcacttg
     1081 tgaagcggct atttctaaat cttccatatt gataaaatcc actattcctt catttgtacc
     1141 tgcttgatac gttttttcca tttcaatgcc ttccctctat aaaaaattga atgaataata
     1201 tgatagcgtt tacacacttt atattactct tccgtattga tacaggcaaa taatctgttt
     1261 acatttcagt cattccagtt gttactactc tgaaagaaat gtttagaaca caaatagata
     1321 aaaaacgatg ggactttgac aaaagtcgtg tcaaagtccc ttttacgaaa tactgtggat
     1381 gcctattgaa ataagtgcgg gttgtttctt tgtatttcaa tcgtttcttg ccattcttcg
     1441 tttaattttg gattaccgtt ttcttccttg aacgcataag gtcccaatcc aaaaagctca
     1501 gttagtattt gattcacgat tccatatagc tcggtgtgat cggacacatg attattgaag
     1561 atcatctcat tatcaccacg tttgaatctc acattcgggc tctcatgatg aacctctatg
     1621 cagttttccc ctttttttaa ctgacggatt tctgaaaaca tctcataaat atcttctcca
     1681 ataaaacctt cagcaagcgc cagactgtcc acaagaccgc ctactgtcaa ctcagagatt
     1741 atcgctttgt tggcaccaag ctttaatgta tagcttgttg cttctactac gccgcatgca
     1801 ttgcaaccac cttctgatag ttcgcttttt ctgatcaatt gaacttcttt cattttctgt
     1861 tcctcctctt agttttctac ttatgtattt tttatcacct gacgtaagtg tattttatcg
     1921 tcacgatcac gtgatttatt cttcagagat tctatttatc tatctaacat atcctttatt
     1981 cttctacttt tctttcttta gcatataaga gatacgcata gtaatcaagc aaggattgtt
     2041 gatataaaaa gaacaaatac cgctattttg atttcttttg actctattac tcatataaat
     2101 aaaatttatt atacctgctc tttttgacta tatcctcatc tgactcctat acttcgtttg
     2161 tattaaaact taggaggaat cattcatgct tacagggcta aacttattaa ataatccttt
     2221 tctaaataaa ggaacagctt ttacaaaaga agaacgggca aaatacggaa tcactggtat
     2281 gcttccaagt actgtccaaa cattggaaca acaatctgtc caagcttacg gtcaatactt
     2341 aagcaaacag accgatttgg aaaaacgtat cttcttaatg aatctattca atacgaaccg
     2401 gacattgttt tataaattga tgggcgaaca tttagttgaa ttcatgcctg tcgtttatga
     2461 tcctgtcgtt gcggattcaa tcgaacaata caacgaaatt ttcttgaaac cacaagatgc
     2521 tgcatttctt tcaatcgacg aaccagaaaa tatcaaagca agtttgaaaa acgcagcaga
     2581 tggacgcgac attcgtttga tcgttgtgac agatgcagaa ggaatcttag gtatgggtga
     2641 ctggggtgtg aacggtgttg acatcgctat cggtaaattg atggtctaca ctgctgcggc
     2701 tggtatcaat cctgcacaag tcttgcctgt atcaatcgac gctggtacta ataacgaaga
     2761 attattgaat aatccattat acttagggaa tcgtcatgct cgtgtagaag gcgaaactta
     2821 ctacgaattc attgatcaat tcgttcaatc agctactgaa ttattcccag aattgttatt
     2881 acattgggaa gattttggac gaggaaatgc tgcaacgatc ttagaaaaat acgaagataa
     2941 aatcactact ttcaatgatg atatccaagg cacaggaatc gttgtactag ctggtgtatt
     3001 aggtggattg aatatatcag gtgagcaatt gaaagatcaa acgatcttga catttggtgc
     3061 tggtactgct ggtgtaggta tcgcaaacat ccttttagat gaaatgatcc gtcaaggtgt
     3121 acctgaagaa gaagcacgcc aacatttcta tcaagtagat aaacagggtt tattgtttga
     3181 agatacaaaa ggattgactc ctggtcaaat cccattcgct cgtaaacgct cagaattcac
     3241 taatgctgat gaattgacaa acttagaagc agttgtaaaa gcaatccatc caacgatcat
     3301 gatcggtact tctacccaac ctggagcatt cactgaagaa atcgtgaaag aaatggcggc
     3361 tcatacccca cgtccaatca tcatgccttt gtctaatcca acaaaattgg cagaagcaaa
     3421 agcaaaagat ttgattgaat ggacggacgg aaaagcttta gttggaacag gtatccctgc
     3481 tgctgatgtg gaatataacg gtgtaactta ccaaatcggt caagcaaata atgcgttgat
     3541 gtacccaggt ttagggcttg gtttgattgc ttcaacagct acacgagtaa atgctgaaat
     3601 catttctcaa gctagtcgag cattaggcgg aatcgttgat gtaacaaaac caggagcagc
     3661 tatcttgcct ccagtagcaa aaatcactga attctctcaa acgattgctg aaactgtcgc
     3721 aaaatcagtt gttgcacaaa aattgaatcg tgaagaaatc acggatatca aagaagctgt
     3781 tgaatctgct aaatgggttc ctgaatataa atctttagaa gattaagatt gttatagtaa
     3841 aagaacatac agaaactttg tatgacgtct aaaaagaaaa aagcgactgc ggcaaaacag
     3901 cagccagcca actactgctc ttttgccgct tctctttggc ttttttcata cgatacagaa
     3961 taagaaagga tctgtaaaaa tggcacaaaa aacaacaacc gcaactgcag ttgaggaaaa
     4021 gaaaaaagaa gggttttggt caacaaaaat taccggcgta agtttgcccc tttatttgat
     4081 catggtcctt gtcctgatca ttgcaatggc tttaggaaaa ttaccgacga atatgattgg
     4141 cccaatcgct gttttggtca ttttcggtaa tttatttcat tttatcggaa caaaaatccc
     4201 tatcgtcaaa agttatttag gtggtggctc cgtttttgcg atctttgcct ctgccgcgat
     4261 tgctacattt gggatcttac ctgagtatgt agtaaaatcc acagaaaact ttgtcagcaa
     4321 tatgggcttt ttagactttt atattgctgc cttgattact ggtagcatcc ttggtatgaa
     4381 ccgtaatcta ttgatgaaag cttctgttcg ttttattccc gttgcactta tttcaatggt
     4441 cgtctccttt tttgccgtag gactcgtagg tatgcttatc ggaaatggtt ttgcaaattc
     4501 cgttttatat atctcattgc ccgcaatggc tggtggtgtt ggagccggag ctgtaccttt
     4561 atcaacgatt tatgcaaatg ttttaggtac ggacgcttct tctatcattt cccgcctgat
     4621 tcctgcgaca gcaatgacaa atgtcctagc tatcattggt gcagcgttag ttgctcgtgc
     4681 gggacaatca atgccaaaat tcaacggtaa tggtaaatta atggaaaaag gcgaccttgg
     4741 cgatggaaaa cctagaaatg taaaaccttc cattccacaa ttaggtgtcg gtttgatggt
     4801 atcattgact ttcttcatct taggacaaat ctgtaactat tttgttccta aagtccatgc
     4861 ttacgcattc atgatcatta tcgttgttat ctgtaaaatc gcaaacctat taccagaata
     4921 ttacgaagat gcagctatca tgttcaacaa cttgattgtc aaaaacttga cagcagctgt
     4981 tcttgccggt atcggtattg cgttattgaa tctaaatgta ttggcttctg ctttgacatg
     5041 gcaatttgtt gtcttatgtt tgacaagtgt tatcgcgatt tcagtagttt caggatttgt
     5101 cggtcgtttg tttggtttat atccaatcga atcaactatt actgctggct tatgcaacaa
     5161 ctcgatgggt ggtacaggaa atgtagctgt cttatctgca gctaatcgga tggaacttat
     5221 tgcttttgct caaatgggca atcggcttgg tggtgccatc gttttgatca tctcaggttt
     5281 cttgatgcaa ttactttctt agtcaaaaaa aatacctgat catcaagtat cggcttgatg
     5341 accaggtatt ttttgataaa aatttacagg attttcaaaa atcttttttg cattttatcg
     5401 gaatgataac cgatgcgttc ataaaatcgg tgtgcttcta ctctgtattc tgcagaatta
     5461 agtcttattg ctgatattcc tcgtcttttc gcttcttcta cgccagtcat caaggccttt
     5521 ccgattcctc ttttttcagc atgtttagac acagcaagtg ccataatatt gaacatcgga
     5581 tcgctgaaaa tcgattcata ggactccgct tgtacatagc cagctgcttt tttagaagta
     5641 ggatcttcat agaccaagaa aaaatttttt tctggctcct tcaacagttt cttcatttgt
     5701 ctcaccgtaa cggttaatgg tacatcatat cctaattgtt ccgcattgat ttctcgtagt
     5761 gcttctgcat ctcctaaacg catttcccgc aacatccaaa tcaccagcca atttttattt
     5821 ttttaaatga atttttttat cacagtatac tccttttttc atttagaaca atatgtttca
     5881 gtgattttga cttgcgttag tctagattct cctaatgttt gtaagatgac gaaagcattg
     5941 taaagattga tcaaaagaac cacgaggaca attacttgaa aaataaaggt cacttttttt
     6001 ggagacaaaa ccctgctaaa tcttgctccc cacaaacctc caataacagc agcggggata
     6061 acataaaaca acatagataa atcaaagcgc ataaagctac ttgttaaagc aatcgttacc
     6121 aattttgcca gttgagaaaa gaaaatcgta gatagagaat atagtgttgc ttcttttatt
     6181 ggcaatgcaa acatcagcat caacaaagaa acattgactg gtccaccacc aatacctaaa
     6241 aaactagcta aaaagcccag aaccccccca catataaggt accaagtcca tgacgtcaaa
     6301 tgaaattttg gctgatgatg cttggtgtag aagaacgcaa aaaccaaggt caataccgtc
     6361 aaaaaaattt ggatcagctg cacatgcttc tcgttttcaa ataactgtaa aaaaacttcg
     6421 aatacaatgt tgccgactac tcctccaaca acagctccac tagataccca accgacaatc
     6481 tgccaattca gtgatttccc gcttgatact tgtcgcacag tcgaaacaat ggacatcgtg
     6541 aataccgcaa ctgttgagta aaaagaaatc cctgcaacag aatgggcgcc gataagatca
     6601 agaatcggtt tgatcaacac gcctccaccc atcccggaaa tagcgccaac tgtgttcgcc
     6661 agaacgatca ccacaaaata aatcagacca atcatcttgt atttcctttc tcttgtttat
     6721 atcttttagc gattgttact cttttctttt attatacagt aaaagaaaca aacgaggctt
     6781 cgaaagcttt tctacttttt aaggaaattt agtgctaaac gaaaagaggt tgtgaaaaag
     6841 ctgtcctgca tcaagtaata agaacagcca aacaaaaata gctcctcata attttcgttt
     6901 ggttgaactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag aggtcgtgag
     6961 cctgccgttc agctccgagt cacaaggaac aatttcaaaa aacagcttct catgtttgtt
     7021 gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttaagg aagagactgt
     7081 gaagtcaacg ttttgacctg attattagag aaacaaatgg caaaatagtt cttcatattt
     7141 cgccgttttt cgagcgaatg ccgaggggca gctgattgaa catcgtttat ttggaaaaag
     7201 ggattgtgac aagacgtttg tcacaatccc tttttttata tcttattaat tcattctttt
     7261 gttctattct tcttcttccg ccatgaatga gaaacccata tcaaagaact ataaaggtta
     7321 tcaaaacagc gtttatgact aaatgaatta gagttatcta tcactatttt tcaagcgatt
     7381 taaaccaaat ggcatacaat atggcataca aaaacttcaa taagccgtgg gggtttttaa
     7441 atttcttctt gttctttaaa atcaatgagt ggagctacat aatttgaatc cactttttca
     7501 aacgtcactt tttgcatcga cgaatgtttt ctttcccaag ataacgtaaa tagatttgac
     7561 tctggactta taaagtctac ttcaccttct agtattactt tcttatgcaa atattcctct
     7621 aataaatata gacttgctaa agcatacaac acattttcca aatttgcttc cttataattt
     7681 gaagaacgac catgttttac tctgttatat attttccacc aatctggtgt atcttcaggc
     7741 cacccatctc caaaaggttt tagtgaagtc aatgttaaag aattcataac cctaacacca
     7801 ttatccaaac acattaattc gctagattta ataacagctt taaaatcagt tatgctttta
     7861 ttgctactgc tatcaaatcc gcaaagttct ttgaaaataa tctcacactc cgaaccaatc
     7921 gctagtagta gtttttgata ttcaatagag aatgttttga aattattctc atcgagcatc
     7981 acaaaatctg tggtattaat aaactcattt tctaaatgta gataatactt ccaataaaaa
     8041 tttgtaaact ctgctttgtc cattaaatca cctcttttga attataacaa aaaaaacacg
     8101 tccacaacag acgtgttctt tgatttctac acaatcagct tgccaaaacc atccaatcag
     8161 tcctagcatg cctgtattaa aattgaacgt tcatcatcta tcatctttct tagtccagca
     8221 tgtttgcaac cacacctaca attttcagtc caacaataga tttcgactag ctaagtcttg
     8281 caaaattcag gcagctaacc cttcatttac taccttaatt atactaaata cccacaacgt
     8341 ttacaagtgt aatcattcta agaaatctgt ttagctatga tcgttgtata atctttaaac
     8401 gccgttcctt cattgattga aataatttta taactcttgt ttttccaagc tactttcata
     8461 tcgtttgtta gttcataatc ttgatcgtat cgaataataa acgtcaacgt tccttcaaga
     8521 atggtaccta cactagcaag cacgtctttt agcatttgtg aacgcacatt agcccaacat
     8581 tcaaatacag tttcttcact atccaccatc tgcccatctt catcttttac ccgttttgtc
     8641 tttttaaact tgactctctc agtcaattca tttacattct taatgattgg cataataagc
     8701 acctctcatt tgttgtacta atgcttgtac cgttactggt atccgttcgc tgctggcttc
     8761 ttgacggttc aaataccaat gttgagtaag taatgacacc gccaaatcaa attgctgata
     8821 attatccata tctttatccg ttgcgctact atcaatagca ctttttatat attcgcctgc
     8881 tgtatcaatc aattgttgaa taaagtcatc atcaatagtg tgatcgatcc gcatactttt
     8941 ttttatgcta tccaaaatta ccattttatc tctcctaatg aaaaaaaggc agtgggataa
     9001 accgccgccc aatatttttc tatacttttg gtgttacaac tggcgtaaac tcaatataca
     9061 ctgaagcatt ttgatcaatt ttcttataat cgttgcgaac gatcactgca agtccttgtg
     9121 agtagtaatc gaatttttcc cattgagtcg ttacttgatt tctacgagca acaaagatag
     9181 attgtgcaat atcccccata atcataggga atgttccagc tttaggattg gcaaacaatg
     9241 tatctgcaat caatactact ggcataccga ataatgattt accactaggt gctgtcacat
     9301 cgggttgtaa aatgtaacgt ccatctgaat ctttcaatgt atctaggtgg taatatccgc
     9361 tttggtttag aattaccatt ttatttaatg ctgggtctaa tgccacattg tatagttgtt
     9421 tcaaatcgtc caacgtagca gccgttttct tagtgaatgt ttttaataaa tccatgatat
     9481 gcttattgtc agtgttttct accaatttcg ctaactgatc tttgacctct tgtacaatat
     9541 tcactgctga atcttccaca acctcatttg ataaggcaat cttaccagca cgagtttcta
     9601 ctttataatc aactgaagta aacatttcag cgtcaatatc accaatttca gcaagttcag
     9661 cttttgttgc taacactgct tgttggttag ttgctactgg atatttacct tgtccatttg
     9721 atactgtttt tactgttgca tattgagcta agttataatt tgaacgtttc aaatcaaaga
     9781 cttcaccgat tacttcttcg ggaacaactg ccgctgcatt tacagtagtg actccatcac
     9841 gcacttcgcc ttgtgaacgg atatattctt catacccacg caattctgtt ttttcgttat
     9901 cgattaaagt tttttccatg ttattactct ccttttgtgc tttagaagtg gattctaagc
     9961 tattgtttgt ttgttttgct tggttgttac tcataaacga ttcgtaagaa cgcttattta
    10021 cttggacatt tgatgaatca taagcgggaa cggtcacgac actaatttca ttaagtgctt
    10081 tgattttatc tattgatcgt tcaattgtgc catcttcttt tttgtcgaat gaatctaatc
    10141 caagcacgaa cccaaaggac atagaatcaa caaccccttt tgagatattc tcatacacat
    10201 catttgaata ggtcgtgtcg gttagctctg cttcaaaatg taatcctgta tcatcgacat
    10261 tcaatttcaa cgtacctgct ttgacactag ctagtggctt tgaataatcg tgtccatata
    10321 ataagaacac atttgataaa tccacctctt ttaatgcttc gggtgtaatc acttctacaa
    10381 agccgcctaa atcttttgac ggttgcccga atttcaacgc atagcctgaa atagttttgc
    10441 cgtctgttgg ttgtttttca ttttctttct ctactttggg agaatcggct gttagctcag
    10501 cttcttctgt cagccgtttt tccttttctt cttcattatt caactggtgt aactccttcc
    10561 tgttctttta attgatattt ctccaacgta tctaaatacg taaagttcaa acttgctagt
    10621 aaacgatcgc caccatccat ggggggtagc cccattttcg accgcccctc attaatcgtg
    10681 agaagtgacc ctttgacctg atccaataca ttttttactt ttgtttcagg atcagtctct
    10741 agtaactgat cggaattaaa gcggatattg gtttcaagtt tcgtatctaa ttcactcaca
    10801 aacacattaa agtaatgaat cagcgtactt tggatatact ggagattact ttgaacggtg
    10861 ctagaatgtt cattttcaac gcctaaacgc tcaatcggta caccaaacgc cttggcaatt
    10921 tgttttgtgt tccaatcatt ggaattgata agcttcaaaa catcggtgtt tatttctaat
    10981 gttttgtagt ccattgtttc atcaagaatg atcgttctaa gagcattatc tccactactt
    11041 gaaccgttgg cttcttcaaa cttttcacga attgcgttct ttgctgatcc gtctaaatct
    11101 gacttatgaa ccttcaaaat acctgaccca ctgacaccac gagtaaagaa attgtgcaat
    11161 gtgcggttgc cagcttgttg aattttcagt tcatcacgta acgcataaag tggtggtaat
    11221 cctgttaaac catcttgggt gaaatatttg aagtgcaaaa tatcgctaga cttcacacgt
    11281 ctttttttat caccaatctg ataagacaac gtaccattat ctaattgagt aaccgttaca
    11341 cttgagttag gtaataagtg aatttcttct accttttcac cgttgcgttt aatctctgca
    11401 aagctattac cgtttagcaa catattgacc gccaaagcaa actttaaatg ccaaccatcc
    11461 atttctgaat taggtttctc gtttagtaac ttcaccagtt cattatcagc ttgtggcata
    11521 ttgtttttga ccaattgaat cggacttgaa gcaatatcac tagcaataat tcgaacggct
    11581 gcaaacacat cactatttct taatgcacga acgcttgtaa agctggtggt gtaatcatcc
    11641 gattggattg ataccacatg ttgtaggaaa ggatctcccg ttgtattttg tctcgcttta
    11701 aaaaatgcca tcttttaacc tcccttctgt tctgtattta gcatgaccgc taccccaata
    11761 agcactatgc ccagcgctaa caagccatag tagacatttg ttagtaaggt gatagcaacc
    11821 aacacacaca ccaatccaag aagtaaaaga acggtatgaa tgtaattaga aagaaaattc
    11881 ttcacttgta taaaattcat tgtctgcttt cgctccttcc gttgtggtga agtaatccat
    11941 tgcaaacaca taggcattga ttaatgcagc tattggatca attttgttac tgttctttga
    12001 tttattaatc tgaatgccgt tgttatccgt ttttaaaata gcgttgttca ctgcatgggt
    12061 gagaatcgta tttttattgt gaataacgtt gccttcataa acttgttctc taaaagttcg
    12121 ggtcggaacg tttagagtaa tcgttccttg tcttacttct aacattgggt agttggcttt
    12181 ttcagcttta ctgataagcg aattagcgtt atacgggtca taacaaatcc ccatacattc
    12241 caaatcattt tcttgaatca aatcttgaat aaactgaaat acttggtcat agtccacgat
    12301 tccactttct aattgagtaa tggaacactc accagctttt tctagttctc gataaggcaa
    12361 accgtcgcgc ttttctttgt cttgaagccc atatttggtg gctacaaagc tatgtgaatc
    12421 acaataaagt tcgccgttat ctagcggtac gatccatgaa acgctagtta aatcatctgt
    12481 tttagataaa tccactccga tataaacggg cttgcctgta atatcttgtg gttctacttc
    12541 ggttgcctgc cagtcttcag caatcatata gctgtcctca ctcgcttgtc tccataaatt
    12601 gaagtttttc accaatacag aattaaggtt attttgtttc aaagcaaggt tcacatcatc
    12661 ttgaatggtt ggaatcatta cttttttgat ttcttcactt tcaaaaatcg gattggcttt
    12721 gatccatttc tcttgatcgt gaatttcttc gggatcgtcc aattcccata tcgctataaa
    12781 gtacctgtcg gcttgttctt cgccttttag cacacgatct aacaataagt attcctcgta
    12841 cataggaacg tttaaatcaa gcccagcggt actaataaca cacaataagc cgttcttttg
    12901 ttgggtcatt cctgacttaa tgacgttgta cgttttacga gtttttgctt cgtgccactc
    12961 gtcaagaatt gcggttgttg gtgcaaaccc atctagcgtg ctggtttcac tagccagtgc
    13021 catagcgaac gaattagagg gcaagtgctg aatcttagag ttcataattt tcagttgtgg
    13081 tcttaaaaac ttactagact tgaccacgtt tctaagtgaa ttagacagca tatcatagcc
    13141 caatttagct tgtttcaaag cgttacttac aaacaatact tgccttgctt ctgctggttc
    13201 ttgttccata atcaacgcat tggcagccat gccacttgcg atatacgttt tcccattctt
    13261 acggctcata ctggtaaacg ctcgattgaa tcgcctgtaa ttcccttctt tagtacgcca
    13321 gccataaaga ctgcctagaa tgaatttttg aaatcctaac atcttgattg ctttaccgtc
    13381 cgtagatggt agcaactcaa tgaatttaat cgctttggtg gcttgttctt catcgaacac
    13441 ataagggaag ctatctgact tagaacgctc caaatcgctt atatgacgct cacacgcttg
    13501 gataattttc ttgggtgcaa tcagcttacc tgataggact ttctcaatgt aatcattcat
    13561 gaattcatca actctttgaa cgggtcttcg ggttcatcat ttttaacctt gttcaaagct
    13621 agttttgatc gtgattctaa ggtcatacct agtgaggtgg cttgtttttt caaatcactc
    13681 atagcttgtg attgaacccg aaccgctgga ttggctttct ttactccgtt ttcgcatgtg
    13741 ataaacgtac ctgattttct tatctcggct tcggctgttt tcattcgagc aaaggctaaa
    13801 caataggcta ccaatgaacc ataatctgct tcactcaatg gataatcttt ttttaatgtt
    13861 ggtacgatac gattccattc acttctagcg ctcgctgcca tccagtcagg gggcgtgata
    13921 tttattaatg gttgctgctt gaacaattct tctctagcgt ccacacggat tgctttttct
    13981 tcacttgata tattgccttt tgtatcgtgt aatattttag ggtgtcccat gttgtcctcc
    14041 tttcagacaa aagaaaaagg aacgtaagca attaagctca cattccttcg tttttcgatc
    14101 agaatttttc tttatctaat ttatctatat aaatgatttt tccgttctgc atttgaatgg
    14161 tcacttgacc aaaatgaggt aactcaatgg tgcgctgttt ccctgtttta tcagtaataa
    14221 caattgtatt tttcaataaa tctcctactt tcattatata ctttttcact tgtgattaca
    14281 agtgtaatcg ttatttgtaa ttacccttat gccttttcac aacggttatt tgtaccaaaa
    14341 tgagtaggca tcgattgtca gtaagtttca cactatgccc ccttaactca aagggtactc
    14401 ttttctcgtc tttgtctttc attggctgtc ttggaattat ggtgtgcatg gcataaagaa
    14461 cgaaaattag tttcatctaa ccgtctattc caatcatctt ttagttcaat tttgtgatct
    14521 acaacatcag ctttccttat caagccttct tctaggcaat cctcacatac tggatgattt
    14581 aaccgataca agtatgaagc ctttcgccat gttctacttt gatagaattg aaaatattta
    14641 ccttcgcttt gctttctttc ttcacgctta attgccgttt gctttgcttt atgcttccca
    14701 caatacttct gcctataatc aatcaatgtt ttacatccag catgattaca ttgtttttta
    14761 actgccatag attaccaacc aaagcgatct cgttcgtcag aatcatcttg ttcccatttt
    14821 gatttgtatt caatataagg cgaaactatc ttcaagcacg ttccgtcttt aaaattaact
    14881 ttagtgattt tatcttcctc aaaactaata aagtctacat cttcttgagt gtttgaatac
    14941 tcaatgtgtt tctcaccatc gaagtatcta atactttcca tatgcataat tattctacct
    15001 ccaataaatt agttatcaat tcatcatctg ttagtagcaa agccaagaac ttatcatgga
    15061 tgtttaaaaa caaattatcc tcattcgatg taagctctcg ttgtctcgct cgatcatata
    15121 ggttaagcag acaattaaat tcatccatta gctcacctgt atcatcatag ttgccactat
    15181 ctaccaatga tcccactact agcgattgac accacgctac tgactcctgc agccaatcag
    15241 gttcttcttc cattatcttt aatacttctg gatcaaatgt ggaataatct tctgttacca
    15301 atcccacact aaatgccagc gtgttcatta gaaaccgatc catttcatct gtatattgaa
    15361 agttcggtgc tttgtttgtt gaaataatat tgagcattta ctttcctcct tgattcgtaa
    15421 cacgtggtaa cacctacgta acaggtaccc gttaccactc aatcccaacg gggacaaggg
    15481 ttctagcgtt ttggtaacag gtaacgggta aaatccagta acattattcc ccagcatatt
    15541 agctaactat ttcaaaagtt ttaaggaaat aaggtgttac cccgttacca aattgctgta
    15601 tcccttgggg cagtaagggt aaactagtaa cgggctaggt gttactgacc cgttactacc
    15661 cgttactttt tgtactttcc agctcgattt ttattttcat aggcatttga cttattgtaa
    15721 aatcctcgtc gtttatttct ttttggcagt tgcttccgtt cccaaccagg aatactgcta
    15781 ataatcaagg caatcttttt agtttctgca tctgatcgac cattcaataa atcctttgct
    15841 tgtttatcaa aaacagcttc caaaatatca gcggtgagta cgctatcaac taaataaaac
    15901 tcttgttgca tagtagctcc attatagaaa tgctgtaaat ttttctcatt acatttactg
    15961 tcaggatagt tctgataata agaacgtctc gcccacgctg gagcttcttc ccatttatac
    16021 gggatttcca tttccaaata tttagtgatt gcttctttaa ttggattctc catcttggca
    16081 tcctcttgat agtcttttgc aatttctaag acttctttat cttcatcggg actaaaatag
    16141 attcgttgtc ctttatcaaa aagcaccttt gcttcagcta acacttgaag aaaatattca
    16201 tcctttgttt taaacggatt catctttggc ttattcttac aaggcaaagg ataaaaacgg
    16261 cggtttcctg tatcatcttt caaatactgc ccatcattcg acgttccaat aaatacacat
    16321 tgccttgccc aagctatgac attccgacca taaggaggtc taattttatc gatcttggca
    16381 ctgatgaagt tttttatctg ttcaatttta gttttgttca ttgaagataa ttctccaagt
    16441 tcaataatta cattgccttg aagctgcata taatcatctt tcttttcacc tagcgaatct
    16501 aatgaatcgg tgaaaaaatc atccgtgtaa agtaatgctg gaacggtact cttaccaatc
    16561 ccttgcggtc catccaaaac tggaacgatc tcaaacttta ttcctggttt atagatacgt
    16621 gcgactgatc cgactatcca acgttttgtt acttctcgtg tgtaaaggtt gtcgtctacc
    16681 cctaaaaggt caataaacag tgtttccagt ctaggctgtt gatcccattt aactttttcg
    16741 atacgttctt ttactgggtt gaaaacattc tttctagcca catttactac tgctttgtgt
    16801 attttgttgt catcaaaaac tacattgtag ttttcttcaa tatatgccgt taaggcatcc
    16861 tcaaaactgt cttcccattc tccagcggta cgattgatcc aaggagaatc ttttaatagg
    16921 tgaatgtatc cgctaaattc gttataagca attgttccag ctaaattgct atcattggtt
    16981 aaaatcaatt ctgcattcac aacactgtta cttttaatct gatcgtcttt attgcatctt
    17041 aatcgtgaca tccaggtttt caattcaatg atattgtctt cacgctcgct ggcttcggga
    17101 aagtctaaaa tactatctaa tttatgctta tgagccatga gctttccccc tcaaatcttt
    17161 tttaatcatc gatcgaataa tttgattcac ttctttatct tttagcggtg gttcattgta
    17221 cgcatttgct gtataaatca attctgctaa agtttgatta tctacaccgt gccatagtaa
    17281 ccaaccgcat aacttagtta aatgatcgtt tctactacct tcgccagcgc cttgaacaat
    17341 catatctaat ttttttgctg tccctgtttt tgctacgggt cggtattgag tctttatttc
    17401 ttgcggtttt tttagcagta ggttcactag ccaatcggga gctggtgcaa tattatttag
    17461 agatacgttt tcaataggtt catatgctct cccaacactt cttgaaataa tcatagttgg
    17521 cgctattgga actcctttag tgataatatc aatcccgctt aaacttttac ctttatcatc
    17581 acgcccgaag atatcagcaa atgcacctgt ttcttgttgg ataattacac catcaggaat
    17641 tttaaagaag aaatggattc ctttacctgg tgtgatttcc gtataagtag aaggaaatgg
    17701 atcatacatt gaagcaagtt ttttataatt ctcaacgccg tttcgtgttt cagaatgttg
    17761 atctaggtca actaccacca tttgatgatc ttcaagcatt aacccaatat ttctagttgc
    17821 atcttccgtc caccattcat taatttgatt agaatcgtta gttgcatcat tccaaccact
    17881 agagcctttt attggaattt tagttccagg agataaggga taaattgacc atcccgactt
    17941 ttgtaattcc gttgctgctt gataattatt tgccaataaa cctctcctcc tttttcaaaa
    18001 tactggtata aatcattcag acacctccat gagcttcaac tgctcaagtt cgtatttcaa
    18061 cccatcccga acaaattcag caccttttaa ttgattctga tactttaccc atgtattaaa
    18121 tgttttcacc cttgcttctt gcatatcagt aatgtttaac catgcggtta aaatctgatt
    18181 ttttagctcg ttgatttctc gacaaattat tttgtgttct gcatgttttt tgagtacata
    18241 caaaactgtt agtttttcat tgaatgaatt tccaaagtag gttttcccga tattattttc
    18301 tagttcagcg atcccttgat taatttcctc taattcaagg ctatcgagtt ggtttaatag
    18361 ttttgttttg tacatctact tatgccctcc agttcttaat tgatagtcgt actcgtcata
    18421 agcagtgaag agatataacg ctaatcctgc aaagatagct atttttgcaa ttaatggtaa
    18481 taagccgaaa attccgccga ttaaaatgct tcctataatt aagtttcgtt tcataatggt
    18541 tccctccgat tttttaatat ggtataatcg gaacaaagaa tatcgggttt tatcccaatt
    18601 aagttgcctt tatcctgtcc gccaagactg ttatggataa ggctttttct ttgttctcga
    18661 tcatgttaat gctcctaagt tgtctgcaat tcagcagaac gattataaat tttctcactc
    18721 actgaaaact gcaacccata tttttcttta acagtcatta attctactgt ctcatctaaa
    18781 ataggttcac gatcaaccaa catttcagga gtcatatctt tcttttttac cattttggaa
    18841 tgaccataaa tagttgatac agctttattg gctatcacat tcgctttaat gtagtctact
    18901 ggttttggtt cagtaatagc gtggcttaat ttagtcattg cttctttttg gtgttctttg
    18961 tctagcatgc ggaatacttg aaagccttca agtcctgtgg actggcgtag ttctttgatg
    19021 atgtcgaaaa tccagtcttg aaatgcctcg gcttcttttt tacggctttt gaatgccatg
    19081 cggtaaatat tttttttcgt tgatgatgtt tacatcttgt gttctgccca atgaatcgat
    19141 gaccttacta gtagtaaccc catctttagg caaagagtga atggctctag taacctgttt
    19201 aagacctaac gcttccgcaa catctttcgc cactgcccac cattcatcat taatatcgac
    19261 aaatcttata atgtgtccgt tccaaatttc agttttcatt caacgtcctc cagttcaagc
    19321 cctagcaagc tcgctaacaa ctcaacttgc tgatacaaaa tttcttgata ctcttcaata
    19381 gaaagtcgtt ctttaaactc tgcttgtact aaatcgttca agccacgcaa tactccgctt
    19441 accgttggtt cttgtggtag caatttttga tagtccattt gttcgtacaa acattcaaac
    19501 gtttcttcta gcgctttgcc ttccaagttt gctaattctg ccgctgtgtc ttctgtaaga
    19561 attgttgata atactttttc gattgcttta ttttgtgtcg attccattat tatttcctcc
    19621 tattaatccc tttgccccca cggcttgcgt ttatttttgg tattcttcca taaagcgatc
    19681 acattcatct tgtgaaattc ttacgactcc atcaactata atcaacttta aaccgttagg
    19741 aacatatttc tgcatcatag tattgtatga aacgttcata tacttagcag cttgtgcctt
    19801 gttcatatat tttggaaact ggtacttttt ttctaataat gaaagtaacg ttccttgagt
    19861 ttgctctact tgttgaacaa ctcgatcgtc aataatttta aaagctggat catcaagcgt
    19921 gagtttgtca atttttaatt ccatatttat cccccctcgt tagcatacat ttctatacaa
    19981 agattttaaa aaaatgcaag aggctcttga atacatttct atacctctta taaactcaat
    20041 ataccatcat accttttcat ttgcaagaat tttaacatac ttttttgaac ttttatgttt
    20101 cacacatatt taaatcgtga tatagtagag aaaatatatg aatggtggtg aaaatgtggc
    20161 tagaaaaaaa taccccgaag cagtggagac aggacaaaga attagactgg ctagaattga
    20221 aaagggactc tcaatggaac aacttggagg taaattatcc ccaccggcca gtaaaggtgc
    20281 cgttagtaat tgggaaaatg gctataatct tcccaataac accaggctta aacaactttc
    20341 aaaggtatta gatgtatcca ctacctatct attaaacgga tcttatacat taagagatat
    20401 gcatatgatg cccgaaatta agcgaaatgt gcttgaaaag caattgaaaa aaactattga
    20461 aaaaaataac tctataattg attctaatct ttctaatgct atcaataaca tcgaagaaga
    20521 taacctgact gtctcacaga agatttattt aacaaacgca ataaaatatc ttaaaacagg
    20581 atctcgaatt gaatttcctg acgggagtac cccaattgct tatctcgcag ctcttctaaa
    20641 tacgctttta cgatatgact cattaattaa tgattcagaa aaaaatacag atgagaaaaa
    20701 agagtatcaa gaattttatg aaaatgatct tctaaaagag ttcacagctc ttgttactga
    20761 gctaaataaa cactattcaa aataataata aactgcctcc tccaaccgcc cccacggctt
    20821 gctacactat ggagggaaaa ctattatggc aacattcaag caatatgaaa ctaaaaaagg
    20881 taaattttgg ctctatgaag cctatcttgg aatgaacaaa atgactggca aacctgataa
    20941 ggttcgtaga cgaggttaca aaacaaaaaa agaagcccag cttgccttgt cacgcttgca
    21001 agtggactac gataaaaacg gtattaaaaa agcgaacaac gaaacgttta aagaagttta
    21061 tgacttgtgg ctagaaacat ataaaaccac tgtccgagaa gccaccttca tgaaaactga
    21121 aatcaagttt agaaagtgga ttcttccaaa atatggaaat cttcgtgtga atgaagtcac
    21181 ggtaaaaaaa gcccaacaaa ttgtaaatag ctgggctgaa actactgacc aatacaaggt
    21241 acttcattca acttctaagc gtatttttag ctacgctatc aatcttggga ttattgagac
    21301 taatccacta gacaacgtat taatgcctaa aagaaccacc actaagcata aagaaaaggt
    21361 caaagtttac agtaaagaac aactgatcac cttgtttgat tacctaaaca gcaaacctga
    21421 aacttatcga aacgactttg ataaaacgtt actacgcttt ctcttttata gcggggttag
    21481 gatcagtgaa gcccttgcat tgaattggtc agacattgat tttcaagaaa atacggtcac
    21541 catcaataaa acactaagcc aatccaaaaa tggctataaa atcagtgatc ctaaaactga
    21601 ttgtagtgct ggggttcttc cactagatga aagaacaatt tctatattaa aaaaatggca
    21661 gattaatcag cggaaatata tgctgacact tggtgtaact gatccgacta tgattttttg
    21721 tggtatctat aaagaactaa tcacacacca cgctatttat gcaagattga ttactatttg
    21781 tgaaaaagct atggttccat ttctcggtgt gcatgtagca agacacactc atgcttcaat
    21841 gctcttggac gctggagcta ctatggtaga agttcaaact agattacggc atacaaaaat
    21901 tagtcagacg atcgattgtt acggtcactt ggcaaaagaa acgaaagaaa agactgttga
    21961 aaaattagta caacacttaa atattgacta gatggcatac aatatggcat acagtaaaaa
    22021 ataaaaaaag gagaaaccct atcatatcaa ggtttctcct agctttatag tctattcttc
    22081 ttcttccgcc atgaatgtat tgaatacttc ttcaatcatg tcccattctg cgtctgtttc
    22141 gatttgttct aactcgcctt ctgttccatc ttcgttttcg atgtatgcat aagcttgtaa
    22201 ttctacatct tcatcttctg aaacaccagc aggatataaa agaacatagt tacgtccaaa
    22261 ttcttcttgt ccgtcaactg ttagtaggat ttcatataat gtttcatttc cttggtcatc
    22321 caccaatgtg atgtgttcgt gtccttcatg gtcgtgggaa tggttatgtt ctgtcataag
    22381 attacctctt ttcttttatt attaaaataa ttgcttacct gagccgtcta aataattttg
    22441 taagatcatc acagctgcta atttatcgat gacttttttt cgtttggctc ttgaagtatt
    22501 tgcctgctct actagcatac gttctgcctg aacagtggtt aatcgttcat cttgataatc
    22561 aactggcaag gagaatagtt ctttgatctt ttctccgtat gacatggaag cttctgccct
    22621 tggtccgatg gaattgttca tatttttagg taatccaact acaaaccggt ctacttcata
    22681 ttgtgctact aattccttca accgatcaaa gccaaactct tcttctgctt cattgatccg
    22741 aatgatctct actccttgag ctgtccagcc aaaggggtca ctgatagcaa cacctactgt
    22801 cttagaaccg acatccaagc ccattactct cataaattca ctccatgatt tgagaggtag
    22861 tccttgacaa ttacttccat gatctcatcg cgttcgtgac ggcgaatcaa atttcgagca
    22921 tcacgataac gaggaatgta ggacgggtct ccagaaagca agtagccgac aatctgattg
    22981 attggattgt agcctttttc ttccaaagcc cgataaacaa tcgctaatgt ttcgctcact
    23041 tcttttttgc gactatcgtc aaaaccaaaa cgtacggttt catctgtaaa acccatgatt
    23101 ctacacctct ttcccgcaag gtaattccag cttaccttct acaaccttat tttactcgaa
    23161 gaaagggaaa actacaaaca aatattccaa acttccgaat aaattaagaa acactatttg
    23221 tatgtttttt tccttttttc tgtagcttta tcaaaagtac tcttttcata aagtatacgc
    23281 tatatttctt tgaatgtatg tttttacaca attttttcat aatattcata aaaaattcga
    23341 atagctgcta ctaatttttt gtttttgttg cttgtcctgt ctcataattg atcgtgtagc
    23401 catcttgaac attgtagata aagacattga attcaatttt tttgtcttca atgctttctg
    23461 cttccaattg cactcccctg gctaccagtt cattcccctc aaaatgagga gtgacacggt
    23521 agcgtacatg atgatttgtt tctttgatat atgccgcaat atcattttcg tgagcaagca
    23581 tgtatggagc attcaacgaa cgtgtccctg tcatcaagtt tttaatgttg ttattttgac
    23641 cagtcagctg gaaaccaatt aaatggctgc gattatataa ccattgcccg tcacttaatt
    23701 tcttttgttt ccaacctgtc gggtcaatat acaaaggctc tctcttttca gtagggaaca
    23761 aatcttttcc aagcatagca ttggcttgtc ctactctgtt caatttatca atatttgaat
    23821 agctttccca tgttcctttg gaaaggctaa gatcctcttt tgtaaattcc ggctgattcc
    23881 cattgatttc tatttcttga tgttttccat catattctgg cacatccgct acttgagaag
    23941 cttgtggtgt ggaatcaaaa atagttgtaa tctgctgaaa gatatctgaa tctttttgtg
    24001 tggtggaaac accgaatgcc cctgcaatca cagcagcgac gatccccacg atcgccttgg
    24061 ttgttttttt gctcatttct ttactccttt actaacagct ccctaaatct taacatatgt
    24121 tcccctataa ataaatacta gaaagaaata gaaacagcaa aaaataaaac ctgttttaag
    24181 atggcttaaa aatacaaaaa agagacggat tcctcgtctc taatcagtca ccactctatc
    24241 tgtaaggtat ttattattat tcaaaaattt tcttcatact caagacaata cactgatttc
    24301 acaaccgctt ggtaaacggt gcgcaaaagc ttgcatctgc ggtaataagt ccatccaagt
    24361 taaaaacata agaagttgtt tttgcttggc tgttcttatt acttgttgca gagcagcttt
    24421 ttcacaacct attttaagcg ggtgacggga atcgaacccg cgacatcagc ttgggaagct
    24481 gggattttac cactaaacta cacccgcaat acaaaaacag tatacgcctc ttttattcct
    24541 tcctcaagtt tttttattct ttacgcttct ggtatgtttt gtagtaaaat aaaactaatc
    24601 aatcaattat tggagaagat gctatgacgg gaaaagaatt agatctgttg ctggacactt
    24661 gtttactcgc tgggaaaatc atgatggaaa gcaatgcgga gatgtatcgg gtagaagata
    24721 cgatgagtcg aattgcttta gcttctggaa atttccgttt ggtcagctat gtcactcaaa
    24781 ccggtctctt tattggtctt gatcgtacct cgaccatccg aatggaacaa attaccaacc
    24841 gttcgattaa tttagaaaaa gtagtaaaag taaatgatct ttcacgtaaa tatgttgctt
    24901 cagagctaac attagaagaa ctttactgcg aactgcaagc aatcgaccga gaccgttctt
    24961 tctttcctgt ctggttgcaa atcgttagcg cggcagtgat cagtggaaca attatggtgt
    25021 tatttggcgg aaagatgacc gatttacctg tgactttagt cattggagga ttgggatacg
    25081 cagcttatct atatagtttg aaatttttta gaatcaaatt tttatcggaa tttttatctt
    25141 ctttattgat tggcatagcc gctatctaca gcgtacgttt tggcttagga agcaatcagg
    25201 atctaatcat tatcggatgt gtgatgcctt tagttcctgg agttcaaatt acgaacgcta
    25261 tccgtgattt gctagctgga cattatgtct caggagtttc tcgcggaaca gaagcgatga
    25321 tgactgctac gatgatcggt tttgcgattg cttttatttt ccagcttttt tattaggaaa
    25381 ggaacacaag tatggggacg atatttattc agttttcttt tagtttttta gcgactgcgg
    25441 catttgccgt tattacaaat gtacctaggc gttcattgat ttgttgtggt ctttctggtg
    25501 cttttggctg gatgatctac tggatctgtg ttcaaatagg cggatctgct gcttttggtt
    25561 cattgcttgg ttctttagga gtagcattca tcagcgatct tttctctaaa cgtttgaaaa
    25621 tgcccgtaac gattttcaat attcccggga tggttcctct agtccctggt ggtttagctt
    25681 atcaagcggt tcgaaatctc gttactgaat cctatcgaga agcggctcat tatgcggtcc
    25741 aagcaattat gattgctgga gcaatcgctt taggtcttgt attatcagaa gtctttaacc
    25801 ataacatccg taatttcaaa gtaaagcgtg acgcaatcat gatgaaacga aaaaaacggc
    25861 tttttaaata aacgaagtgt aacaaagaaa aggttgtgaa agagacgttc agccttgagc
    25921 gatgacaaaa tatctcacaa acattgcgac aagtaaccgc aggagcaata agaagtaatt
    25981 atgagtatta aaaaaagttt tgtaaaaata gcttctcaca tttttacata aaattgactt
    26041 aatactgaga aactgacttt tgaacaccct ctatctgtaa aaaaggggct gcgacatgat
    26101 ttttgtcacg gcccctcttc tttattcaaa ttttttccaa aacgttcgtt gataataaac
    26161 gatccataga gcggaaagta ttagaactaa accgatcaca ggaatattca ctaaccaaaa
    26221 tggataaaac aaagccaaca ttccgtataa gacgataaga ataatactga tgatcaatga
    26281 acccatcata gttgcgacta aacccaaact tcctgaacct ctgttaaata attggttaat
    26341 attggtccag ttcagcagca gcaaccgata atctctagcg aaataacgta gtgacaataa
    26401 gtagcagccg aataaagaac caaatagcaa gctagggata aaaaacaaag gcagctgaaa
    26461 agacagtccg cttaataatg cgatcccacc agttaaaaaa ctctgcaata tccatccgaa
    26521 tctgaacttt tcttttaaat attggttcat agagattggc aaagaacgga taaacagaaa
    26581 attttcttga tccaaagaaa taagattgct gataaaagaa gtctgattaa ctgttaagaa
    26641 agccaatgcg atccctacca ggaaaacaac accgattaac ttattatcta aatcagttaa
    26701 ttttatttcg cctcctaggg caaaagcaag aataaaaatg attggagtta acaaggaaga
    26761 agaaagaact tgcataatca aattaggttt ttttactaat tgagcattgt aactaaataa
    26821 aagctgatgc acattttggt tctttttatg ggttcttttt attttgaact cactgtttga
    26881 atcagtcagt tgctctgtaa gctttggtac tatcagctgt tttacagcaa ggaaaaatcc
    26941 cagagccgtg atagctagta ttccccaacc caataaacct tgttgagaaa gtggctgact
    27001 ggcagcatca tagaatggca ataatatcga aatcggatgc cgatctgtta actcattttc
    27061 aatagtcccc gtttgatgat tcatccacat gattccagca aatgcgatca gcatcgagct
    27121 gccaagcaag atactagaaa ctgtttttct atgtttttta aacactgctg ttcgagccaa
    27181 accaaaaaca atcaaactag ccagacaaaa aagaataatc aaaaatagag aaaacaaaag
    27241 gattgagata atgatagtaa gcaaaatccc tagatcagct cgccatccgg ttaaaataaa
    27301 catgactaga agcggaaaaa caaaaggaat gatggtcaaa gcaactacca aaatctttgc
    27361 tgaaaaaacc tgttcttgtc caaaaggcag aggcaaaaat acttgcaggt catttccttc
    27421 aaaaaagata ttataaatga ctgatatact ttgagttaaa cctaacatac caaaaagtgc
    27481 cacgtaataa gtaaagaaac ctggcaattt aacaaagttc atagcaaaca tagcaaatcc
    27541 gtaaatcagt aaaaaaatca gccctgaaaa aagattttga gacaaaagat agatagttaa
    27601 tcgctttccc ttttttcctt tctctcttgc tttattcgtg agctgtggat taacatataa
    27661 aagattaact cgaattaatt ctaataattg tcgcttatac atacggtcca tctcctgtcg
    27721 cttgccttcc agccattttc aaataaatct tttctagcgt ttcatcaggt gagccattca
    27781 ataattcact aactgccccc tgataaatca attcaccttt cttcaaaata gccagtttat
    27841 cgcataactg ttgtgcggta tccaatacat gtgtagaaaa aatgactgtt tttccttttg
    27901 cggcatgagc cttcatcatt tctttcaaat caaatgctgc ttgaggatca agtccttgca
    27961 aaggttcatc caatacccaa atatctggat ctggcagcaa cgtgccgatc aaaatcgttt
    28021 tttgacgcat tccatgtgaa aaacttgcga ttgtttcatt ttgatgacta tacatatcaa
    28081 aaagcgctgt caattctgct aaacgctttt cttttttttg tgtgtctaat tcataggcag
    28141 ctgaaatcaa gtcccaatat tctccagccg tcaattgtaa aaaaatatct ggggaatcag
    28201 gcacataacc gattttttgt ttgatactca atcgattttc cgtcaatttc attccatcta
    28261 ctaaaatcgt tcccgatgat ggttcaataa tactaactaa acttttaatc gtagttgatt
    28321 ttccggcacc attatgtcct aaaaatccaa aaatttcccc ttgtcttatc gagagagaca
    28381 agtcttttaa tgcttgtttt ttcccgtaac ttttacttac attgatgaat tcaatcattt
    28441 ctgtactcct tttgtttgtt attacttttt attgtaccct tcgttattgg tggagtaaat
    28501 gtcaaaaaga gtgcataaca aaaaaggata gaaacgacta ggctgctact tgggttgttc
    28561 tcttctacag gaaaacactc ttctaacagg taagatatga gtctttgttt tctttaaaat
    28621 gattcatttt ctatcttttc ttgcataaaa ttctttagtt tatcaatact tctctttccc
    28681 atctctagtt gttctccatt tgcaaatgtt actatttcgt ttactctgct aatagatgta
    28741 atgttttcag tattaatgat ataagctttt aaatctttac ataaatactc atattcgagt
    28801 gattttttat attttttaat tgtgccgttt acgagaatct cttcatcttt caatactagg
    28861 cctattttat ttttataacc tggcatgctc attagataaa ggatttcatc gtaatggata
    28921 tagtgatcgc tattaccata acggacagtt gtccaccttt ttttcgtagc atgttgaaca
    28981 ggatagtttt ctttttcttt caataagtaa ctttggatgc tttttttatt tttttcataa
    29041 gtgaattctt ttatcaaata accactaggc gtaatctttc gattaataac agttattgct
    29101 ttattcggat gagacgttag ataaacaatt ttacactttt cattcttttc tctaagcatt
    29161 tcgcctagtt cgataccgct ataataggcg ttcaaatcga tgtctaggaa aaaaatatct
    29221 gaggaataga tttcattatt tttaatgaat tcaacaaatt gtataacgtt caatccctga
    29281 acgatctcaa atttccaagg acttaataaa attctggatt cttccaaaat ttctactagc
    29341 attttttcaa aaaccaaact atcatccaaa ataaatatac gcgttaccat aaaaagtacc
    29401 tccactcgat attttactat caaaatcatt tcatacaact tttcaacata catgttagga
    29461 ataaattgta attctcttca caatcttttc caacaccttt gaaaccgata tcaaatactt
    29521 tcaggatgct gcttctaaaa taaatatcaa acaaatgaat acatcatgca aaaaaagcca
    29581 gacaaccgac gtccttgcgt gtcagctgtc tgagctatgt ttatttattt tgttctttta
    29641 aaaagaacga tacacatact gatttttcgg agcagagact ttctcagcgt ttgtaacaac
    29701 tacagatggg atttcacgct tgaatggttg ataatagtct gattggatgg ttccttctat
    29761 tttataccat tcatcatttt taaccgttac tccttccggc atatgcgtca acaaaccaaa
    29821 gactcctgaa tctgccacac aatggataat gccgaatcgg aacaagaaga tatctgtatt
    29881 gctatcgttc ggtgtttggt aaacaaatcc ttcatagctg atttttttgc caatgaattc
    29941 gctagggtaa ttataaatta gctccataac ttctaaatag ttttcatctg tgatcttgat
    30001 agtttgctta tccccatact tttctttcaa ctcattcatt tggtctaagt agtctgattt
    30061 gttgaaataa atactggtat ccggctttaa gtattgtgtc tgcatctccg gatcccctac
    30121 tgattcttta cttactggga aattaaatcc tttggcttct acgatcgtag tgtcgagact
    30181 aactgttgga aacaacgtcc ccacgattac tggcaaagct agcagtacat aagcgattcc
    30241 acgttgataa ggttttgaca acccatggtc atgatcgtgc tcgtgtgcct tttcttctgc
    30301 ttttccgttt ttgacccaca aatacagctg aactaaagcc aaaagagcag ataaaatcat
    30361 tgaaagaata gctaagtagc gataatggac attgatatat tgatcgagtt tgccagaaac
    30421 ttgcaagtac atcatcaatt cgaaataccc agataaaatc aaaaaccgca gcatattttc
    30481 cctcctaaat aaataatgtg aatccaataa cgctaagggc aatcagtccg atcagcgata
    30541 aaataaagcg tcctgtaaaa tatcgtttca tcatcagtag atttttgata tcgaccattg
    30601 ggccaaagac caaaaaggca gctacaggtg ctgtgccgaa cagacttaac aaagaagatc
    30661 ctataaacgc atctgcttct gagcaaagag acatcgtaaa ggccaataac agcatgatga
    30721 taatggctaa aatctttgtt gagccgagcg ttagcataat tctagttggt aaataagtct
    30781 gcatcgcagc tgcgattaat ccgccaatga tcaaataacg acctgtatca aaaaattcgt
    30841 cgattgaatg tgtcaacaca tgcccagttt gatggatcag ctgatttttt ggttcattct
    30901 tctcactttg ttcttcttca tggacatgat gatggtgatt ttctgctact ttagacgtga
    30961 ttttttcttt caatacagcg tctttttgaa aataggcaag ccagacacca atcacaaggg
    31021 caacaaaaaa gctgcctatc acccgccagc ttgccatctg ccaagaattg ccaaatgcga
    31081 taaaagtgga aaaaatcacg attggattga tgatcggagc agtcagcata aaagcaaacg
    31141 ctgtgtgaac tggtacgcct tttttaacaa attgatgaac gatcggtacg atgccgcatt
    31201 cacatgaagg aaagaaaaat cctaagatcg aaccagtaaa aatagagaag atacgatttt
    31261 caggaagcca tctcttcaca cgttctgggg tcaaaaatac ttgtaaggcg cctgagataa
    31321 gacagcctaa taatacgaaa ggtaatgctt caataacgat cgataagaaa atcgtactca
    31381 tctgcaaaac cgaatttgga ataaaagaaa acatgagttt cctccttttt atttctagtt
    31441 cattcgcttt gcaaatatac gcttttattc aaaaaaaaca agttgctctg taaattttta
    31501 cacaactctt attttttacc aaagcacttc aattgacttt ttctgttatt cccattaaat
    31561 taaggtctca tacatatcac ttctatcata aaagggagaa taaaaatgaa acaaaccact
    31621 aacttgactg aagttcaaga catactgcaa cagtcatccg ttgccgtcat ttatctttcg
    31681 atgcctaatt gcagtgtttg tcacgctgtt cgtccacgac tagaggaact tctcacacat
    31741 tatgatattc cggcacttca tttggatgca tttgaaacac cagaagtagc tagtaaattt
    31801 gaagtgctga ctgcgccagt tgtcctgatt tttcatcaag gaaaagaagt ctttcggcaa
    31861 gcacggttca tcgactttaa aaaaatccgt tatctactag atgaactgac agcagaagat
    31921 gattcccttt cttatgaaga aatcttccga acaaaataat cgttgtccaa acagaaaaaa
    31981 gcgattgaac aagtgaaagt tttgccttca tttgttcaat cgctttttat ttttattctg
    32041 ctgtattttc ggaatgcgca tttgcgtcag aatgcaagaa atagatcaag gtttgcaatt
    32101 cattcgtcaa atcaacattt tggatcaata catctgacgg agcattcaat cgagcagcgg
    32161 taaagttcaa gattcctttg attcctgctt ccgttaactg attgactact tcttgtgctc
    32221 tttcagcggg tatcgttaaa attgctactt cgatttgttg gatcttgatt tgttctgaca
    32281 tatcttccat tggataaacg gggataccgt ctacgatgcg cccaacgatt tcctcattca
    32341 catcaaaagc acaactgata cgtatgctat tactttgatg gaatttgtat tttagtaaag
    32401 cacttcccaa gttccctaca ccgatcagcg caacatttgt caattgatcg tcgtttaatg
    32461 tctttgcaaa aaagtgcata agatcttcta catcataacc atacccacgt tttcctaatt
    32521 cgccaaaata agaaaaatct cgacggatcg ttgcactatc tacttgaacg gcttcactta
    32581 attcagttga agacactttt ttctttccag cattatttaa aatccttaaa tagcgataat
    32641 acaaaggcag tcttttagct gtcgcctttg gaataatatg atctttcacg aaatgacctc
    32701 cacgtattgt ctaattacat ctttgatttc tccatcatag cagtatttat aggaaaaaat
    32761 caattccatt gctcagatat tgtgaaattt tattctttat tcatttattt atcatttctg
    32821 caaatctcgc agtataatgg aatttatggt agactactaa ttgaaaaata gttttttatt
    32881 tgaggagaat tcacaaagta gaaaagagga ttccatgata ttattacaag ccaatcagat
    32941 tgctcgccat tttggtgcag aagtcttatt tgaaaacata catttagaaa tcagtacagg
    33001 cgctcgtgtc gctttagttg gcagaaatgg cgctggaaaa tcgactttat taaagatcat
    33061 tgctggaatt gaagcacctg atgaaggaaa gatcgctaaa aataaaacag cgactctcgg
    33121 atacctcgct caggacacag gacttgattc caatgaaaca gtttggaacg agatgctgaa
    33181 agcttttgaa caagttagac aaatggaaga gcggatgcgc gagctagaga ttttcattag
    33241 cgaaggtacg ccagacacac ctgcttatag ttcttattta aaagaatacg accggctgca
    33301 acacgatttt tcagatcgaa acggctatgg ttatgaaaat gaaattcgtt ccgtattgca
    33361 tgggtttaaa ttcgattctt cattttatga tcagaaaatc gatactttat caggcggaca
    33421 gcgcacacgt ttagctttag ctcgcatgct tttgcaacac cctgacattt tgattctaga
    33481 tgaaccaacg aaccatttag atatcgagac actttcctgg cttgagaatt atctgcaaag
    33541 ctatactggt gcattattga ttgtttccca tgaccgttat tttttagata aagtagtcaa
    33601 tgaagtctat gaattaagca gaagaaaaat gcgccattac aaaggtaact attcgaaata
    33661 tctagatttg aaagcagaac agttagcaag tgaatggaag gcttatgaaa agcagcaaac
    33721 agaaatccat aaattagaag acttcgttgc tcggaacctt gttcgcgcct ctaccacaaa
    33781 acgagcgcaa agccgcagaa agacattaga aaaaatggac cggctggatc gtcctcaagg
    33841 ggatgaaaaa caagcaaact ttctattcag tatagaaaag ccttctggaa atgtagtctt
    33901 gcaaatcgaa gatgccgcta tcggatatga taatgtcacc ttatctgaac caatcaatct
    33961 agatatccgt cgtcaagaag cgattgctct agtaggtccg aatggtatcg ggaaatcgac
    34021 gttgctgaaa tcaatcattc accagatccc ttttatccgt ggaaaagaaa cgctagggac
    34081 aaatgtcacc atcggctatt acgatcaagg tcaagctgat ctgcatacaa acaagacgat
    34141 cctagcagaa ttgtgggatg agcatcctac cactccagaa aaagagatcc gaagtgcttt
    34201 aggtgctttc ttattttctg gcgaagatgt agaaaaacca atccctctgc taagtggcgg
    34261 tgaaaaagca cgggttgctt tagccaagtt agcgatgaac aaagaaaatt ttttgatttt
    34321 ggatgaacca acaaaccatt tagatattga caacaaagaa gtattagaaa atgcattgat
    34381 cgattatgac ggcacactgc tttttgtttc ccatgaccga tatttcatca atcggatcgc
    34441 gacaaaagtc gtcgaactct ctgaaaatgg cagcaaagtc tatctaggag actatgatta
    34501 ttatttagag aaaaagcagg aagaagaaga aatcgccgaa ttgttggcac aagatgagcc
    34561 agaatctgct cctgctccca cgaacaaatc tgattattac caaagcaaag aacagcaaaa
    34621 gcttatccgt agccttcaac gaaaaattac tcaggttgaa gaagaaatgg cgcggatcga
    34681 tgaatggatc gatcagttgg aacaagaaat ggtcgcgcca gaaaacctgg aagatcacgt
    34741 aaaactcaat gagctgaacc aagatttaga agctgctaga caagatcaag aaacaaaatt
    34801 gacggaatgg gaagaactaa gcttggaatt agaagaatat gaaaattaac gaaaaaacca
    34861 aaacagaaac cattcatgct gttttggttt ttcttttttt attttatcaa acaaaaattc
    34921 catatttacc tttttcacca ataagaagac caattactag tcattctcac taaagcttct
    34981 aaataatagt aatcacccca aatacaaggt gtatttaccc cattattttt gggcttatta
    35041 taaacagact gtgttaaaaa gccatcttct tcttccgttg tagcatatct ttctttcaat
    35101 tgtcgcataa ttttccaaac agcagactgg tataagttct tttctgtttt gtctaataga
    35161 cctgattcta ttaattctaa taatccacaa accatgatca ccatactaga tgtatctttt
    35221 tcttctctgc tagaatcatc aaagatcaaa tcccaataac aaatggtatc ttctggcaac
    35281 cgattcataa aataattggc taatattttg cttaaatcaa aatatttttg ttctttcaaa
    35341 taacgataac ttaatgcaaa accgtaaatc ccccatgctt ggcctctagc ccaacaggaa
    35401 ttatcactat aaccttgatg cgtacttcca tatcttggtt ttccatcttt gacatccata
    35461 taaaatgtat gataagttga accatcttct cttactaaat aatctgctgc attttgaata
    35521 tgagcctgag cagctttttt atatttttga tttccagttt cttcactcgc ccaatacaat
    35581 aatggaagat tcatgcaaca atccatgatc attctacctt gttgtgaagg atcactagga
    35641 tctccccaag cttgtataac tcctgccttc tcccaaaaac gctgaagtaa tgtttctgca
    35701 gcatcaattg aagcttcctt catttttcga tttcccgtta ttttataatc tgctacggtt
    35761 gataaagaat acaaaaaacc aagatcgtga tgattagtga taatttgaca gtcaatcctg
    35821 tggataaaat ctatatctgc tttttcagct gtttgttgaa ataaaggatc acccgtcttc
    35881 tcaaataaca accaattaat tcctgtccaa aatccttctg tccaaccaat attgtctctt
    35941 aatggataac gtccttttat aggacactcg cttgggaatt gatactgact gttactgata
    36001 cgtttgatta acctctccgt gtgtctgcga caacgctcga tctcattatt ccattctttt
    36061 tccattaatt ctttataaca ttcatttatc catttttctg gatgtattat ctcttcaaac
    36121 atcactttat caagcatttc cccaccagcc tttagcataa ttttagtctt tttttattgt
    36181 ttttattttg gatagttagc ccggttgtcc catcaaaatc aattttggga cgattgttcc
    36241 atacttctaa actataatcc cgatcttgat gagcaaccac tcctgttccg agccaatggg
    36301 ttccttttcc aaatgtaccc tctaatccag gaataaaagt gttttcccat gtcatcaagt
    36361 ttgtattggg caaacccata atagctgtgg attttcgttt aatagtatcg tcacttaaac
    36421 agataatacc tgaaaagcca gcttcactca ccctatagtt ttccttgtct tcttctatga
    36481 ttattccttc tttctcagga gaacattcaa tagcaaaccc tgtctcatat agaacatatt
    36541 ctgtatctaa ttctactttg tgtacgcgaa tctgccagcc ttcaaaagga ataagccaag
    36601 ttctgatttt tgcctgtaaa gtaggttccc atgtagaggc aagtacctct ttcttacata
    36661 caatgtctcg tacattattt cgaacgatga actgtccata ggttttgatg ctctcattct
    36721 taaaactggt cgtgatatat gtgtccgctc gctgaatccc tagcattgaa tccattgcca
    36781 tttctgcttt caattgattc gctcgcggta tcgaaaaacc aaattgacta gaataagcaa
    36841 attttaaata tttttcattg gtcaatgtat ggaaatttgg ccctaattgt cccgcattca
    36901 aaaaaaatgt atgtccatta tcatgttgta tgagcaaatt ggcttctgaa agaagtttag
    36961 ttccttcacg aataggcata ggcatttctt tagccgtcca atactcatgg tcttcaggta
    37021 gcgccaacaa taaaaaaatc ttattcagcc ataatggaga taacgtacta ttatagggtt
    37081 cagtcaatgt caattgaggg taggtataac caatattcag gatacctttt tcatcaaata
    37141 tttctttttg catccaccat cttatattcc gattgactaa ccccttgatt tcacctaatt
    37201 taaaaggcaa aagatcggcc cacaacatag ctgaccaaaa gcttgaaact gcaaaacgat
    37261 aaatcatgct tcgaccaaaa ggcacatttg caccatcttc cgcaaaaaaa tgaatatagt
    37321 ctctagaaaa atcaatagct cggttcttat atatctggct aatgggcttt tctggttcta
    37381 gtacgctata aatcaaacca taataatgaa atgcaaaagg attataataa tcttggcgac
    37441 ctcttgaact gtctcgatac cagccatttt gaagataaca attttctata ttatcaaatg
    37501 cttctttcag tgctgattct ttaactggat aaccaaattt ttggataatt gttccgacaa
    37561 ttatcttaaa aaattgccaa ttaccatctg cacattcata ttcaaaaatt tgcgagaacc
    37621 aatgaataat ctggcttttt tctcctgtat ctaatttttt ccataataat tgttcatgat
    37681 gatataacgc taataacatt gcgggcattt ctaccatacg ctggtctctt tcatgtattt
    37741 ctccccaata atcgggatgg gaaggatctg ttccagcttt tattcctgat agaattaatt
    37801 tcaaccatcg ttcatctaat tggtcaattg cgggccccgc tccccataaa acccgagaga
    37861 atccttccat tcctgcaatt ttatttccat atcctacccc ttgatgtata taatcaattt
    37921 gtgcgccact tgctcgataa aacggctgga gtggttgatt tatcttttta aataagtcaa
    37981 agtaatcctg tctggtcctt aatagtggta ctggatgttt cattgtattt tctcctcttt
    38041 ttactaccaa atagctaatt ccgactgttc taatcgaagt aagccttcaa taaaaaaata
    38101 atcagaatag atcagtggca cattataagt cagttctcta tggtatgctt ctgctccgcc
    38161 tccaatgatc ccatcttcat ccatattcca atttgcatag tgcttttctg tcttttggag
    38221 tatattgata gcagcttcat gatacatttt tgatcgtaca gaggaaacat atcctgctaa
    38281 ttccagaaga ccgcatgcag cgcatactcc agcactggta tcaatattcg aatcttttac
    38341 agcaaaatca atgagaggaa tataaccagt tttttcgatt ttctgaataa aatttactgc
    38401 tatcttttct gctatagcta gatagtacgg attttttgtg taacgataac ttaacgcgaa
    38461 tccatataaa gcccatgcat ttcctctgct ccaacaagaa ttctcattgt atccttgccc
    38521 gccaagtaac cgaatatatt tccctgattc tgcatcaaat tctgcgatat gcccggttga
    38581 tccatctttc ctcactagat acttagctgt tgtatctgca tgctgccgag caattttaga
    38641 gaacctcgga tcaccagttt ccctcgaagc ccaatacagt aatggtaaat tcatcatact
    38701 atcaataatc atccaaccag gtttatggtc attccatgct actaaatagt tccctgaaat
    38761 attatagcgt ccagcaagta ccgtagctgc atgtaaccct agttgtcgac tatgtttgtt
    38821 ttgagactgt tgataatgtg caatagccga tggtaagaat aaaaaaccta catcatgatg
    38881 aacgtctgaa aaatggtaca gggcagattc taataattgt tcttgttgta ttgctgcctc
    38941 tttgaacttt tctcgttttg tcttgtgaaa gagctgccat aaaataccac tccaaaaacc
    39001 attggtccac cagtcgattc ctctgtcctt tattacgtct gtatattttc cgttgtaaga
    39061 aatatatgga attttattcc ccattctgtc taattccgca tctatctttt cagaagctac
    39121 attcaaaatt tttcttgacc attccttcgt ctgttcattc atggatatcc cgccattctt
    39181 ttaattttta tgaatgtgga gttgctccta gaacgaacta gatggttaac attcgttatt
    39241 ttttttactt atttgcagct ccaatgtcat tctccttaaa tttagactgc gcccacacat
    39301 aaatcaagac cattgggatc actgaagttg ctaaaattag cataattgcg ccataattaa
    39361 ttggctcacc tgtactagaa accgtcatat ttttgataat taagggaacc gtatatttat
    39421 ctttatctga aagcatgact aatggaaata gatattgatt ccaacttccc ataaagataa
    39481 gtaaaccact agtaataatc gtaggtgata aaatcggtac agcgattttc gtaaaaatca
    39541 cccactcatt ggcaccatcg attctggcag cctccatcag tgctgtagga aattgactgc
    39601 tatattgtcg catcataaac actgaagttg tactggcaat cgaagggatg atcacacctg
    39661 cataagtatt cgataatcct aaacgactca taatgtaaaa taaaggaatc aatgtggtaa
    39721 aaaaaggcac catcctagat agcataactg tattgaacaa cagtttattc cccataaaac
    39781 gatactttgc taatgcataa cccgccattg tagaactggc tgtagctaaa actgtactga
    39841 ttacagcaat aacaatcgaa ttccaaataa tcaagggata attatatctc gccatgatgg
    39901 cttgccagtt ttttaatagc atatccccaa acttgaaatt gattccgcca cttaaaattt
    39961 cttgattact ttgcgttgaa gctacaatca tatataaata tggcatcaaa aatattaagg
    40021 caccaaacac aataatgacg tacgtaaaaa ttctgctttg tatcttttgt ttagtcattt
    40081 ttatcacctc cgaatttaaa ttgaatagcc gataatataa cagatacaaa caccatcgtc
    40141 caggcaatag cagaggcttt tccaaaatca accatttcaa aacttgtttt atataaattg
    40201 actcctacga ccaaagctga attttctggt ccattggaag tcccaaagaa tatatttggg
    40261 atattgaaat tacccaagcc attgatagtg gctaaaactg tggccattaa aatgattggc
    40321 tttagcatgg ggatcgttaa taaaaagaat tgccgaaatt tcgatgctcc atctatttca
    40381 gcactttcgt atatctcttt agaaatccct tgaagaccag ctaaaaataa aatagtgtta
    40441 tatcctacac taagccaaat catcgtgatg atcattgata ttcgtatcca aaaaggattt
    40501 gttaaccact gtacaccgtt aattcctagc gcattcaaaa atccattgaa taacccagtc
    40561 ggattgaaaa aagtcatgaa tacggaagct acagccaccg ttgatgtaat ggttggaata
    40621 taatagacag ttctaaaaaa tccttttcct tttaactgtg ctgaattcaa taaaacagca
    40681 aagtaaatag ctaatagcat cgttattgga attgatcctg ccatgataat caaaacattc
    40741 gtgtaagatt taataaacaa tggatctcgg aagatagcta gataattgtc aaatccaaca
    40801 aattcaaatg agtagccaat tttcataaaa ctaaaagcaa acgagataat gatgggaatc
    40861 aaaatcgtca caataaagaa aataacaaaa ggtgtgacca atccataagc acttttttta
    40921 tgagacgtat ctgttgcttt tttcttgctg ttctttattg tcttgcttgg taacgaagat
    40981 tgcatcatcg ttaagtcctc ctaatatttt ttaatcacca gttaatttta cgccgtactt
    41041 atgagcaaaa ttttctgctt cttttagata tgaattttca aaatcacctg tttgccaata
    41101 gttatatgca gcggtagtca aataagacgt agcatctgaa taagtggctg gtaaaacagt
    41161 atttgatgta ttttcattga ttttattcaa tagttcatag tatttttgtc ctccttagta
    41221 gtcaaacttt ttctcagctg cttctgtttg ataggccaat gagttagatg tagccacgcc
    41281 cagctccagt gcgttggcta agcattcttc atctgtcagc ataaatgtaa gaaattgttg
    41341 tgccacttgt ggattttttg catctttagg gacataaaaa cttgatcctc ctgatactgc
    41401 accttttcct ggattttctt ttgaaaaagg aatcagtgga gccatgcgcc atttgtcaga
    41461 tgcatcagga aaattcaaag acatattcgt agcataccag ccgccagcaa caaacattgc
    41521 agattcttga aatgctttct cagcatcttg tgcaccgtag aaatgtacaa tatctgagtc
    41581 gatcatttct ttgattaact ttgcagcatg tttcgcttct ttagtattca gacggatatt
    41641 tccttcttca tcaagcaaag attgattctg ctgggctagc atgttgataa acaattcttg
    41701 gtccccagat tctggcaagg caattccaaa actaccagtt ttttctttta atgcttttgc
    41761 cacctggata tattcttccc aagaactgat ttcttcaaaa tctacaccta tttcagcaaa
    41821 tttttttggt tgataataac aaagtgctgg ggtaaaatca ctagcccaag gaataatcgt
    41881 ttcattactc gtttgttgac gcaaaatatt caatttttga tcataaaact gatcgccata
    41941 tttgtcataa aatccatatt catcagcact ataaaaagca tcttgaaatt tttccagcac
    42001 acctgctgct gaagtgtctg ccatggagat aatatccggc atatcttcgt gtgttaccaa
    42061 ttttggggtg atcatttgta ctgttgctgt tcccgaagct acattcacaa tttttaagtc
    42121 aatatttgct ccagttttgc ggttatagat ttttgcagca ttttcaacat agtttgtcag
    42181 tttgccgttt gcgacatata cttctaatgt agtggctttt tttatcattg gatctgcgat
    42241 gtttgctgtt acttcttcgt tagtttttcc acatgcactt aagctgaata atagggtaat
    42301 gtttagcaaa gctatcacca atttgtatct ttttttcatg tctatccctt ctttcttttc
    42361 aagttcttat at
//
LOCUS       DAYYME010000006        39843 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000006 DAYYME010000000
VERSION     DAYYME010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.006"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            71..940
                     /locus_tag="ACHBFW_000249"
     CDS             71..940
                     /locus_tag="ACHBFW_000249"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFM9316597.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            1105..2376
                     /locus_tag="ACHBFW_000250"
     CDS             1105..2376
                     /locus_tag="ACHBFW_000250"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM9316598.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            2373..3668
                     /gene="rho"
                     /locus_tag="ACHBFW_000251"
     CDS             2373..3668
                     /gene="rho"
                     /locus_tag="ACHBFW_000251"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFM9316599.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            3767..4030
                     /locus_tag="ACHBFW_000252"
     CDS             3767..4030
                     /locus_tag="ACHBFW_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFM9316600.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            4475..4924
                     /locus_tag="ACHBFW_000253"
     CDS             4475..4924
                     /locus_tag="ACHBFW_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9316601.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            4981..6399
                     /locus_tag="ACHBFW_000254"
     CDS             4981..6399
                     /locus_tag="ACHBFW_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM9316602.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            6399..7259
                     /locus_tag="ACHBFW_000255"
     CDS             6399..7259
                     /locus_tag="ACHBFW_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM9316603.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            7433..8206
                     /locus_tag="ACHBFW_000256"
     CDS             7433..8206
                     /locus_tag="ACHBFW_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9316604.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            8353..8718
                     /locus_tag="ACHBFW_000257"
     CDS             8353..8718
                     /locus_tag="ACHBFW_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFM9316605.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(8866..10074)
                     /locus_tag="ACHBFW_000258"
     CDS             complement(8866..10074)
                     /locus_tag="ACHBFW_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9316606.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(10093..10371)
                     /locus_tag="ACHBFW_000259"
     CDS             complement(10093..10371)
                     /locus_tag="ACHBFW_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316607.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            10564..11220
                     /locus_tag="ACHBFW_000260"
     CDS             10564..11220
                     /locus_tag="ACHBFW_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM9316608.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(11662..12459)
                     /locus_tag="ACHBFW_000261"
     CDS             complement(11662..12459)
                     /locus_tag="ACHBFW_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFM9316609.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(12456..13640)
                     /locus_tag="ACHBFW_000262"
     CDS             complement(12456..13640)
                     /locus_tag="ACHBFW_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFM9316610.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(13665..14912)
                     /locus_tag="ACHBFW_000263"
     CDS             complement(13665..14912)
                     /locus_tag="ACHBFW_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM9316611.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            15127..16872
                     /gene="dnaX"
                     /locus_tag="ACHBFW_000264"
     CDS             15127..16872
                     /gene="dnaX"
                     /locus_tag="ACHBFW_000264"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFM9316612.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            16892..17206
                     /locus_tag="ACHBFW_000265"
     CDS             16892..17206
                     /locus_tag="ACHBFW_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFM9316613.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            17231..17827
                     /gene="recR"
                     /locus_tag="ACHBFW_000266"
     CDS             17231..17827
                     /gene="recR"
                     /locus_tag="ACHBFW_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFM9316614.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            17891..18535
                     /gene="tmk"
                     /locus_tag="ACHBFW_000267"
     CDS             17891..18535
                     /gene="tmk"
                     /locus_tag="ACHBFW_000267"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFM9316615.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            18550..18879
                     /locus_tag="ACHBFW_000268"
     CDS             18550..18879
                     /locus_tag="ACHBFW_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFM9316616.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            18893..19831
                     /gene="holB"
                     /locus_tag="ACHBFW_000269"
     CDS             18893..19831
                     /gene="holB"
                     /locus_tag="ACHBFW_000269"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFM9316617.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            19867..20691
                     /locus_tag="ACHBFW_000270"
     CDS             19867..20691
                     /locus_tag="ACHBFW_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFM9316618.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            20684..21031
                     /locus_tag="ACHBFW_000271"
     CDS             20684..21031
                     /locus_tag="ACHBFW_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFM9316619.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            21100..21972
                     /gene="rsmI"
                     /locus_tag="ACHBFW_000272"
     CDS             21100..21972
                     /gene="rsmI"
                     /locus_tag="ACHBFW_000272"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFM9316620.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            22081..23202
                     /gene="dinB"
                     /locus_tag="ACHBFW_000273"
     CDS             22081..23202
                     /gene="dinB"
                     /locus_tag="ACHBFW_000273"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFM9316621.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(23256..23858)
                     /gene="nrdG"
                     /locus_tag="ACHBFW_000274"
     CDS             complement(23256..23858)
                     /gene="nrdG"
                     /locus_tag="ACHBFW_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFM9316622.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(24173..26362)
                     /gene="nrdD"
                     /locus_tag="ACHBFW_000275"
     CDS             complement(24173..26362)
                     /gene="nrdD"
                     /locus_tag="ACHBFW_000275"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFM9316623.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            26688..27455
                     /locus_tag="ACHBFW_000276"
     CDS             26688..27455
                     /locus_tag="ACHBFW_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9316624.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            27445..29487
                     /locus_tag="ACHBFW_000277"
     CDS             27445..29487
                     /locus_tag="ACHBFW_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9316625.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            29768..29917
                     /locus_tag="ACHBFW_000278"
     CDS             29768..29917
                     /locus_tag="ACHBFW_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFM9316626.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            29928..31442
                     /gene="dltA"
                     /locus_tag="ACHBFW_000279"
     CDS             29928..31442
                     /gene="dltA"
                     /locus_tag="ACHBFW_000279"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFM9316627.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            31439..32644
                     /gene="dltB"
                     /locus_tag="ACHBFW_000280"
     CDS             31439..32644
                     /gene="dltB"
                     /locus_tag="ACHBFW_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFM9316628.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            32689..32922
                     /gene="dltC"
                     /locus_tag="ACHBFW_000281"
     CDS             32689..32922
                     /gene="dltC"
                     /locus_tag="ACHBFW_000281"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFM9316629.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            32925..34193
                     /gene="dltD"
                     /locus_tag="ACHBFW_000282"
     CDS             32925..34193
                     /gene="dltD"
                     /locus_tag="ACHBFW_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFM9316630.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            34334..35416
                     /locus_tag="ACHBFW_000283"
     CDS             34334..35416
                     /locus_tag="ACHBFW_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFM9316631.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            35416..36219
                     /locus_tag="ACHBFW_000284"
     CDS             35416..36219
                     /locus_tag="ACHBFW_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM9316632.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            36289..37029
                     /locus_tag="ACHBFW_000285"
     CDS             36289..37029
                     /locus_tag="ACHBFW_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM9316633.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            37040..38044
                     /locus_tag="ACHBFW_000286"
     CDS             37040..38044
                     /locus_tag="ACHBFW_000286"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFM9316634.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(38089..>39843)
                     /locus_tag="ACHBFW_000287"
     CDS             complement(38089..>39843)
                     /locus_tag="ACHBFW_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316635.1"
                     /translation="LTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDSKYT
                     RTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPG
                     LSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTM
                     IIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVH
                     SFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPEL
                     MPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQT
                     TINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQE
                     VNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILAR
                     YQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLS
                     IHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKD
                     TFIDRITVQLSRNYPNPV"
BASE COUNT        12774 a         7016 c         8273 g        11780 t
ORIGIN      
        1 taaaataggg tcgttggtta agaattgtct taacaaattt agttttacaa aacttaggag
       61 gaattttatt atgccagtag tatcaggagc tgaattttta aaagctgctc gtaaaggcgg
      121 ttacgctgtc ggcggattca acacaaacaa cttggaatgg actcaagcaa tccttgaagc
      181 ggctgaagca aaacaagcac cagtacttat ccaaacatca atgggtgcag ctaaatacat
      241 gggtggttac aaaatctgta aagatttaat cactgattta gttgaagcaa tgaacatcac
      301 tgttccagtt gctatccact tagaccatgg tgattacgaa gctgctttgg aatgtatcga
      361 attaggctat acttcagtaa tgttcgatgg ttcacatcta ccatttgaag aaaacttgaa
      421 attagctaaa gacgttgttg aaaaagcaca tgcaaaaggt atctcagttg aatgtgaagt
      481 tggttcaatc ggcggagaag aagacggaat catcggtaac ggcgaattag ctgatattga
      541 agaatgcaaa caaatggttg ctacaggtat cgactactta gcttgcggta tcggtaacat
      601 ccatggtcaa tatccagaaa actggaaagg attagctttt gaccacttac aagcaattgc
      661 tgatgctgta ggtccagaca tgcctttagt attacatggc ggatcaggta ttcctcaaga
      721 tcaaatcgaa aaagctatct caatgggtat ctcaaaagta aatgttaaca ctgaattcca
      781 attatcattt gctgcagcta ctcgtaaata tatcgaagct ggtaaagata aagaaggtaa
      841 aggattcgac cctcgtaaat tattagctcc aggtaaagca gcaatcgttg aagatgctaa
      901 atctcatatc gactggttcg gttcagctaa caaagcgtaa gcatccacat aacagatacg
      961 atatatagga atggagaacg acttttgaag tcgctctcca ttctttttta cttaaaacgg
     1021 cagaccatgc tcgtttcttt tttttatcta tgataaaata caagcatatc catgaaaaaa
     1081 gattaataaa caaggtggaa acagatgaaa aaaatcgtaa tcaaaggaaa ccgaccttta
     1141 gctggtgaag taacgatcag cggtgcaaaa aatagtgcag tcgcattgat tccagctgca
     1201 atcttagctg attccccagt gatcttggaa ggtgtgccgg acatacaaga tgttcattca
     1261 ttaattgaaa ttttagagat catgggcgca actgtccatt tttccaacaa catactagaa
     1321 atcgatcctc gagaagtcgt atcgattccg atgccgcacg gaaaaatcaa tagtttgcgt
     1381 gcttcttatt attttatggg aacactgctt ggtaaatttg gcgaagcggt agtaggcctg
     1441 ccaggtggct gttatttagg ccctagaccg attgacctac acgtcaaagg atttgaagct
     1501 ctaggtgctg aagtgacaaa tgaacatggt gccatgtatt tacggacaga aaataaagga
     1561 ttgcgtggca atcggatttt catggacgtg gtttcagtcg gagcaacgat caatgtgatg
     1621 ttagctgctg taaaagcaaa aggacagaca gtaattgaaa acgctgcacg agaaccggaa
     1681 atcatagatg tggcaacttt actgaataat atgggggcaa aagttcgagg agcgggtaca
     1741 gatgtgatcc gtatcgaagg agtcgaaaca ttacatggct gtcggcattt catgattcct
     1801 gaccgcatcg aagcaggaac ctatctagca ttagcagctg cagtaggaaa tggtatcaaa
     1861 gtgaaaaatg tgatctttga acatttggaa agctttatag caaaattgca ggaaattggt
     1921 gtccatatga aaatcagcga agatgaaatc gaagtttacc cctcgaaaga attaaaaccc
     1981 gcaaacatca tgacctatcc ttatccagga tttgccacag atctgcagca gccacttact
     2041 gcgttactat tgatgactac aggcacttca gaaatcatcg atacgatcta tgcgaaacgt
     2101 gtgaaccatg tgccggaact agcacgaatg ggcgctgata ttaccgtgga aggaaatatg
     2161 atcatcgtga atggtccaaa taaacttcac ggcacagaag tcgtggcttc tgatcttcgg
     2221 gcaggtgctt gtttagtgat tgctggacta ttggcagaag ggacaacgac gatttacaat
     2281 gtagattata tcttacgcgg ttatgatcat attatcgaga aactaacagc cttaggggca
     2341 tcaattgaaa tgatagagga agtgggagct gaatgagcga ttatttgaca atggcagaac
     2401 tagagaacaa aacgttaaaa gaagtttata gttatgccaa agaatttaaa attccttatt
     2461 acagtaagat gaataaaaag gaattatctc tagctgtgat tcgtgcacaa gcagagaaac
     2521 aaggatttta ttatatggaa ggaatattgg atatcgttgg tcaagatggt tacggttttt
     2581 tacggccaat caattatgga ccaagtgcag aagatatcta tatttctgcc tcacaaatca
     2641 gacgattcag tttgagaaat ggggacaaag tggcaggaaa agcccgtcca cctaaagaat
     2701 cggaacgtta ttatggtttg atgcacgtgg aaagcgttaa tggaaaagat ccggaagaag
     2761 caaaagaacg tccgcatttt ccagccttaa cacctcttta tccgcaaaga cagcttcaac
     2821 tggaaacaac accgcaaaga ctatctaccc gaatgatcga tatcttttct ccaatcggtt
     2881 ttggtcagcg tggattgatc gtcgcaccgc caaaagcagg gaaaacttct gtattaaaag
     2941 aaatcgcaaa tggtatcacc gaaaactatc cggaagtaga attaatcgtt ctactgattg
     3001 atgaacgacc ggaagaagta acggatctag aacgaagcgt gaaaggcgac gtggtatctt
     3061 ctacatttga tctgcagccg caaaaccata ctcgagtttc tgaattagta ttggaacgcg
     3121 caatgcgttt agtcgaagat aaacgagatg tcgttatctt gatggatagt attactagac
     3181 ttgctcgggc ctataactta gtcgttccac caagcggacg tacattgagt ggcgggattg
     3241 acccagccgc attgtataaa cctaagaaat ttttcggggc agcaagaaac atcgaagaag
     3301 gtggcagttt gacgatttta gcgaccgctt tagtcgatac aggaagccgt atggatgacg
     3361 tgatctatga agaattcaaa ggaactggaa atctagaatt acaactttca cgtgaattat
     3421 ctgaacgtcg gatcttccca gccatcgata tcaaaaaatc aggtacccga aaagaagaat
     3481 tattgatgcc aagtgaagaa ttagaagaaa tttggaaact tcgaaaaaat atgactggag
     3541 attcattaga atatacggaa caactgatcc gctttttgcg taaaacaaaa aacaatcagc
     3601 aatttttcaa agaatttcaa gatgtctcct ttggtaaaaa aaatccgacc agaaatctga
     3661 aaagataatt gcaaccttga ctgaaacatg ttaagatgtt aatgttgtaa atcgacaaat
     3721 caactctggt tcagcaaatg gagcagggca aaggagcgaa aaaatcatga aagaaaacat
     3781 ccatccagat taccacccag tagtattcat ggattcaaca actggtttca aattcttgtc
     3841 aggttcaaca aaaacttcac aagaaacagt tgaatgggaa gacggtaata catacccagt
     3901 catccgtgtc gaagtaactt ctgactcaca tccattctac actggacgtc aaaaattcac
     3961 acaagcagac ggacgtgtgg accgtttcaa caaaaaatac ggtctcaaag acgcaaacgc
     4021 tgcggaataa taaaaacgct gataactcaa cgtttagcag actatctgat agggatagtc
     4081 tgttttttta tgttttttgc caatttaaaa cttcgtttga tgctgaaaaa gtcgtaggat
     4141 cataatgcac aaaacaatga aaaactagta tatagtttta attatccact tgataaaaac
     4201 accttatatt aacgaaatga agaggggtta tttcgaagta aaaaaccatt tacctaaaat
     4261 tagaagctaa gctttccttt caaggaaggc tttttgtttt gttatagact tgcgattgtt
     4321 tgtgagctag atcgttcatt ttaatgactt atacacgcgg atgatgggtc tttttcctct
     4381 gttcattcat aatattgaac ggtacagcta gcgatgctat actgacctca gttccaagaa
     4441 aacgttaaca ttttttgtgg gggagaggtt ttttatgagg tttgatcaat tgatcgcaga
     4501 agaactgatt gttttcgatc catcgataga ttcaaaagga tcattatttg agcgaatagg
     4561 ggatgtgttg aaaaaaacaa ataaggtaag aaatgttcaa aaagtcgtta aggcactatt
     4621 caaacgagag gaggagatct ccacaggaat agaagatgga tttgggattc cccatgcaaa
     4681 aagcaaatat gtgacaaaac cagcactgtg tttttttcat acagccaaat taacggacta
     4741 tagcgggata gatgaaatgc caattgaata tatttttgcc atcgtagttc ctgaaaaatc
     4801 agcagatatg catctagaga ttctcagcac actctccaga cgattgatga atgcagattt
     4861 tagaaaggga ataaaagaag ccactacgcc agcagatatc atcagacaga ttagtcaaga
     4921 atgaaaatag tagagtcttt gtccgagtat cgatagtgaa gaaaaaggag gaaaataatc
     4981 atgaaaatcg ttggtgtaac tgcttgcccg actggaattg ctcatactta tatgtcggca
     5041 gaaaaattag aagtaacagc aagaaatcta aaccatcaag caaaattcga aacacaaggt
     5101 gtaaaaacag aaaatcagtt atctgaacaa gaaatcaaag aggcagatgc aatcattctt
     5161 gcagtagata aagaaatcga attggaccga tttgccggga aaaaagtaaa gcgtgtctct
     5221 acaagccgag caatcaaaga accgcaagta gtaatagacg aagcattgag agatattggc
     5281 gtattcgttg tgtctaatga aaaagaacca gctgcaaatg agaaaaagcc gacaatctat
     5341 aatcatttca tgaatggtgt gaattacatg cttccatttg tgatcgcagg cggaatcatc
     5401 atcgcactga gttttgcctt tgggattaca gcggctgatc ccaactcagc agactataat
     5461 gtgttagcag ctgcattttc aaggatcgga ggagatacag cgtttgccat gatggttcct
     5521 gcgttaggcg cggggattgc tacatcgatt gctggaaaag caggttttgc cccaggaatt
     5581 gttgccggat tactggcctc tacaggtggt tcaggttttc ttggcggaat gattggtggc
     5641 gtgctagcag ggtatatctg tgattttctg gcaaataaaa ttagcgtaaa aaaagaattt
     5701 tctgctattt atcagctgat tgtcgtcccc tttttatcga tcttaattat tgggcttttg
     5761 atggtatttg tgattgaaca accgatcaca tgggtgttgg atgctttgac aaattggctg
     5821 aatagcttgg gaaatacatc cgggctattg ttcggattac ttatcgggat catgatggca
     5881 gctgatatgg gtgggccgat caataaatcg acttcgactt tctcgatcgg tttgatgtca
     5941 gctggagtaa atgcaccgat tgctgcttgt atggctgcag gaatgacacc tcccctaggt
     6001 attgctttag ccactttatt attcaaacat aaatttacca aagaagaacg gacttcaggt
     6061 caatcatgct ggattttagg cgcttcgtat attacagaag gtgctatacc gtttgcggta
     6121 tctgacccga tccgcgtgat acctagtttg atgacaggtt cagcagtcgc tgcagggatt
     6181 tccatggcag taggagttac ttcaatggca cctcatggag ggatttgggt gatgctaatt
     6241 ccaaatgtta tgaacaattt acctatgtat gtcttggcaa ttgcatcagg tacagtcact
     6301 acagccttta tgcttggact attgaaaaaa acagtgaaag agaaaaaaca agacaagtgg
     6361 acgaaaaatg aaaatctaac aaatgaggtg gaagcgtaat gttatataca atgaaagagt
     6421 tattgaaagt ggctaaagag aatcaatttg cagtaccagc cttcaatatt tgcagctatg
     6481 atatgatgaa agcaatcatc gaagaagcag agagattaga agcaccagta atcttagaaa
     6541 ttcatcctga tgaaatcgcc tatttaagtg atgagtttgt tgcggcagta agagcctatg
     6601 cttatacaag taaactgccg atcgttatcc acttggatca tggcggtacg atagaagatg
     6661 tcttacgggc gattcgaaac ggctattctt ccgtgatgat cgatgcgtct ttgaaatcat
     6721 tcgacgacaa tgtacagatt acgaaaaaag tggttgcgtt agctcatgcg gtaaatgtct
     6781 cagtggaggc tgagcttgga acgattggga ataatggttc tgctgaagga ggtgctgaaa
     6841 caatcattta tacagatcct gatcaagcga agatttttgt gaaagaaaca ggaatcgata
     6901 cacttgctgt agcaattgga acagcgcatg gcttgtatcc gaaaaacaga actccaaaat
     6961 taaatatcga cttactgaaa gaattgaacc aacaaatcga tcttccattt gttttacatg
     7021 gcggttcagg taatccggac gcagaagtca gcgaagcagt aaaatacgga gttgccaaag
     7081 tgaatttgag ctcagatctt aaaagcgtct ttttcaatgc tttgagagaa atattgagtg
     7141 aaaatccatc gatgtacgag ccgaatgcaa tttatccagc agctaataaa aaggtacaag
     7201 aagtggtcaa acataaaatg gaaattttga acacagtagg caaggctagt ttatactaaa
     7261 aataaaaatg tgtggtcaag agggtatgac aatagtcttg tcataccctc ttaaccacac
     7321 acgatgttca gaagtagttt tttcgaaaaa tttcttctta tttctcgaag ccaatcactt
     7381 ctgtcacaac ctcttttttc ctgttactat ataaagtaaa tggggtgatt ttatgttgaa
     7441 tgaagtggag aatagacagc aggagattgt gaattatcta aaaaacaacc aatttgcaag
     7501 agtcaccgat ttgatcgagt tagtaaatta tagcgaagcc acgattaaac gtgaccttgt
     7561 ttttctggaa aaaagtggat tgatccgtag agttcgcggt ggcgccatgc ttattgacaa
     7621 tcgaaaaatt gacgtgcctt acctgatgaa gatcaccaaa ttagaagaag aaacagaaaa
     7681 acgtaagatt gcagatacag cttcttcttt gattcgagat gatatggtct tattcttgga
     7741 ttccagtaca acctctttac acttagtcag aaacctagca agatttgatg gtttgcagat
     7801 cattacgaat ggcatcgtca cagctgctat gttgagtgaa tatacaacgg caaaagtatc
     7861 tgtattggga ggggcaattg ttccgaaacg agcaacaatc aacggttcaa aagcctataa
     7921 cgatgcattg tcatattatg cggatttagc gataatgagt tgtcgcgggc tagatttcag
     7981 ccaaggagta acagaaactc atgaaggaga ggcaatggtc aaaaaagctt ttagaaaaca
     8041 atcagaccgg cttgccgttt tagttacaca gcaaaaaatt gcacataaat tcatgcatca
     8101 agccattgct tttcatgaaa tcgattatct ggtgacaagt aaagaactga caaaagaaca
     8161 gtatgcgaaa gtaaaggaac accatatcga atgtctgtac ggataaatta ttacgtgaat
     8221 gttctaagtg gcttcctcat ttttagatag gaccttatct ttgctcgctt ctctcagccg
     8281 atggtataat aatgaaaagg ttatcttgtt agataatctt acttttttat ccaggagaaa
     8341 ggagtgcaga aaatgaaaca tgtgaaggcg ttggtagtga aggcaatcat gatctgggcg
     8401 attctgtggg tcgtgctgac aggtttatac ggtgttagct ttatggattc aacgattgtt
     8461 ggggtaatta ttgtagtcat gatctatgtg ctaggggatc tgttgatctt acgtaaagta
     8521 gggaacatcg cagcgacgat cgcagatgct ggttcagctg cagttatctt gtggctctat
     8581 ctatatttta tgaatgatac agtcgatatt tggatgaaag tattgattcc tgctctattg
     8641 atcggtgtgg cagaatggtt cttccacaag tggttgctaa gtcaaggcat cgtaccagat
     8701 gaacggaaaa tggaataaat caacagttaa actattctta tcgataaatg aaagctttaa
     8761 aaataaatag atgtttaatt aagaataatc ataaactatt tttactcaaa aaaccgctca
     8821 gattattttc agatctgaac ggtttttttt atctattcat caagatcaaa tttcattttt
     8881 caatgattca atcaattcgc cctctgcttc atgactatgt tttcggccat taacaagata
     8941 gagtgctgta catatcaatg gaatgactcc ggcaatcaca tacaatcctc taaatgaagt
     9001 aagagatttt agcatcccca tcaaataagg accaacgcct aagcctaggt ctaaaccaat
     9061 aaagtaggta gataaagcaa tactgattcg atgttcgcgg acgattttta aacaaacagc
     9121 ctgtccattt gacataaacg taccataacc taggccgact aatgctccag agataagcaa
     9181 tgtcacacta ttcgtcgtta cactcagtaa aaataatcct gctgttaaga ataaatagct
     9241 aggatacatc acgtattgtt ctcctttgac atcgaaaatc cttccagtca atggacgtgt
     9301 aaatgtgatg accaaagcat atacgacgaa gaaaaatgac ccagcagcaa ctaagtggat
     9361 tacttttgta taagaagata aaaatgataa aacacttgaa taagaaagac ccatcaaaaa
     9421 ggcaattcct gtaatgaaaa gtgctttctt ttcaataaaa ctattgatat tccatgagcg
     9481 aagagatatt ttttgttctt cagataatgg aatatttttt acaggaaaga caaggcaggc
     9541 aatcgtaaca aagaagacca gtacaattga aaaccagata atgaaacgaa aatcagtcat
     9601 attcagcaaa atcattccta aaaatggccc aacagcggca gctaaactag tacttaatcc
     9661 ataataattg atgccttccc cttttttaga tgcagggata tatgcagtaa cgatagcatt
     9721 tgtagctgta gaaactgtac cgtagccgaa tccattcaag aatcgaacta aatacaatgc
     9781 cccgatagtt ggcgtataaa gatatgctaa tgttgaaagg agatagaaga tcgcgccgcc
     9841 tcttaaggta aacttccgac caaatagttc taactgtttc cccatgaata agcgtgccag
     9901 caatgttccg atgatataga tcccagaagc aaaacctgct tggctcaagc tggcgtgtaa
     9961 ggtgtcttgc gcaatgactg ccatgatgac catcaacaga taataaatca ggtagacaat
    10021 aaaattgatc aatgtgattc caataaaata tttattaaat aatttctttt ccatgtatgt
    10081 tatacattcc ccttaagctg aatatgcttt taatttttgt atttgaactt ctacatcgtg
    10141 ataattggat ggatagcgat caacaagttg gcgaataaat aaatctcttg tagacataaa
    10201 cagatttaaa aattcttcgc attcgtttgt tggattctca tccactgctt tgatcaattc
    10261 gatcgagttt tgatacgcat catttaaaaa gactaaagaa gctttcatat caaatcttgt
    10321 ttgatatgct gtttctaatc gattaatgat tgtgttcatc cagtaattca tgttttttcc
    10381 ctctattctt aaaaaatttt acttctgcag tataacgaat aaaaaaaagg aaaaaaatcg
    10441 cataagtgat ggaaaaaggt acttaacaaa tgttaatatc attagtaaaa tacctttgga
    10501 ctttaaaaag ttttaaagta ctgtatgatc tttttagatg aaagtttgga tgggggagca
    10561 tcattggata gtagagtctt aaaggaatat ttggataaaa atgattttcc gattatacgc
    10621 aaaggatatc ataaatattt gacattcgaa gggatggaag attcccatac gtatatcttg
    10681 aaagagggaa cggtaaaagc aagtgttatt tcaaatgatg ggagagaatt taaccttcgc
    10741 tatatcaaag atcttgaaat cgtctctctt ttaaaagacg aatattcaca attcatcgat
    10801 tcgccgtata atattcgagt tgaatcagaa tatgcagagt tttatcgaat caatcgtgtg
    10861 cgcttttggc aaaatatcaa ccaagaccca aagctacaac actatgtaaa agagtattac
    10921 cgctatcgtc tgctttattc gatgaaaaaa atgcagcaaa tgctattaaa cggaaagctg
    10981 ggagctgttt gtacgcaact ttacgaatta tatgatttat tcggtgtgcg gacagaagaa
    11041 gggatgctga tcgatttttc gatcacgaat gaagaaatcg ccaaattttg tggcatctca
    11101 actgctagca gtgtaaacag aattttaaaa caactgaaaa aagatggtat tatcaccgtt
    11161 aaagaccatc atatcgttat tttgaatatg gacttgttag aagacaatat tatcttttga
    11221 tgaacaaaga ggttgtgaaa tcagcgtttt gacctgagta ttagagaaac aaacggaaaa
    11281 atagttcctc atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc
    11341 gtttaccaag aggttgtgag cctgccgttt aactctgagt cacaaggaac aatttcaaaa
    11401 aacagctttt catgtttgat gaatattgtg acttgtgacc gaggagttgg taggagaaca
    11461 ccgtttacca agaggttgtg aaaaagctgt tctgcatcaa gtaataagaa gagccaagta
    11521 aaaacagtct cttatgtttt taacttggct ggacttatca ccgtagatgt aagcttttga
    11581 acgccgttta tttgcaacaa aaggggctgt gacacgattt ttgtcacagc cctttttttg
    11641 cgaacattca tttagcaatt tttagaaact tctgccgcca ccgccaaaag tcccgccacc
    11701 gctagaatgg gtagtacttc cgccaccgcc gcttccagga ggtgggcttt tggggattct
    11761 tcttgtcgtg acaaaagaat ttgtcagacg atcggttttt tcagttaatt tgatactgga
    11821 tttttctcgg aatggatatt tgtagatgcc tgatttcaat tgataacgcg aaacagtcac
    11881 aaaataaaag acaagactaa ggataagagc caaaacaaca gcaatgatga tttctactgt
    11941 cgtcaaaacc ttatagcgtg taattttgcc agtttcttcg tctacacgat aatgtcctcc
    12001 tggaactccg tcttccacat atgttgctga tttagacagg taagcttttg cagcattgta
    12061 ataggcagca tttgtcatct gatcataaac atcatctaag atcgaatcga ttctgctatc
    12121 agtcaaataa tcgatcatat taccagaagt cgaaatgtag atttcacgct gtcccatatc
    12181 taatagcaat actgagccat tttgatcatt tccgactgcg tctcttaagt aattatcagc
    12241 aaagtctctt ggttcttctg aattagatgt agtcgttacg atcaagactc gtcctttgat
    12301 tttttcattc agcttttctg cctgttgttc caaagattgg atttgatctg ttgtgaataa
    12361 cccagcctca tcatttaccg tagctgtatc tgcatgagta gccaccggcc agccaacact
    12421 tactagcaaa agaaagaata aaaaaaattt tttcatcata tcaagtaacc tccgatcaga
    12481 aagaggatcg ctagcccagc gaaaatccca agagcggtca gtcccaattt tttctggctg
    12541 atcggcagga caccgctgac ctttcctgtt tgtccattca ttgcgtagta gtagactttt
    12601 ttacttgagt cattgcttcg ataagtaaca agccataccg gcagtaatac gtaattattt
    12661 acttcgtttg tgattgaagc agaagttcgc acaccggtca atgttgtata tccatttgcg
    12721 gtatctttta acagcttctc tgaatagtct tttaattcag attgaatctg tgatttgatt
    12781 gcctcatatt cgatatctct tttttctgct tggaacccgg ctaaatactg gcttttaaaa
    12841 tcaattgctt tatctaacgg gaaaggttgc acgccttcga ccatcttttg ttgcgtattt
    12901 tttgataagg cattttttac tagttcttta aaagaaagct tgcctgctct tgaaacagca
    12961 aattgcttcg tttctgtgta ttcgatgtca cctatccgcc atatgcggat aactgtacca
    13021 ttcgcctgca tggcaccatc tacttcagca tctgtagccc aataagggaa atacacacct
    13081 gtcaacttat cgatttgatc cttattgaaa aaagctttgg gaacaaacca ttttttcttg
    13141 gtccaagcga gaaatctgtt gattgcttct tctttctcga cagcaaaagg aagtacttta
    13201 tttggcaaga attctccgct taaacgtccc gatagtacga cgggattatg gcagtagtaa
    13261 caataagtcg cagcagttgt cgcatcagtc acgatctctg caccacagtt gggacaaaga
    13321 aaaagatcta tcgctgcggt attatctgca gattcgtccg ctgtatctga aaaaccgcct
    13381 gcctcgtcaa ccgctttgcg ctcggcttca ttcatatcgg caagctgttc ttgggctgta
    13441 aaagtaaaat cttcggtagg tgaaggttct tctttgcttt cttcgccagc catacgcgct
    13501 tctttttgct tttgctcata ttggctgact tcttcttctg tatagatatt caggcagtat
    13561 tcgcaatgaa atttttggtc tttgggatca aaagtcaacg gaccgccgca attaggacat
    13621 ttatgtgtaa gaacatccat cagctgcaca cctttctaaa taaattaatt aacggggatt
    13681 cctttaaacg gtttaccaca gtttggacag aatttaggga tgccgttgga aagatccacg
    13741 atctcattgc attcactgca tcgcatttgt agcttaggct gaacgttttc tacaggtttg
    13801 gcagctccgc agttggaaca gaattttccg gaattttctg tcccgcaaac tggacatgtc
    13861 caagtatccg catttccttg tgcagtttgt tgattttgtt tttctgcttg ttgtttgatt
    13921 tgttcttggt tattttgagc tgcttgagaa agataagaac catttgcatt cattcccata
    13981 cctacaccca taaagccggt cattgctcct gcttcatttt ttccggctgc ttccatacca
    14041 cgtgcaatgg aaccttgcac gtagccttca cgtacgctag gatcacctag catcgcccct
    14101 tcgttacgca tattgatcag tttcacagaa tcatcggtgt aagaaatgct ggctacagcc
    14161 acagaaacaa tctccatccc acgtaattca cgccaagaat catccaatgc agtatccatg
    14221 tatttgctta attccaaact cttagatggt acataagaaa tccgttgacc atctgcagac
    14281 atctgattga ttgctgattg tagcgctgtt aggaattcag ctaaatactg ctcattgata
    14341 tcattgattt ccacttgtgt tttgttttta gggattgcgt ttgtataaaa taaaatcggg
    14401 tcaacgacat gaatagaata agtgccatgt gcccgtaaga ataattctgc gttatagaaa
    14461 ttgtcaaagt agttcaacgg cgcgcttgta ccgaatttga ttccctttat ttcttggaga
    14521 ttgatataaa aaacttgttg tttctgcggt gtgacgccgc caaatttgaa tcggtcaaaa
    14581 gtttcagcaa ttgcttcttt cagtgttcca ttgaacatgg aaggagctgc gtcattttta
    14641 atggtgtaat aaccttcctc agcggtataa tcgatgattt ttccaccatc gaccaaaagc
    14701 atcatcatgt ttggatagac atggacgact gttccgtctg ttaaaacatc ttctgttcct
    14761 ttacgattgg agccacgttt gtcatctttg cggactttga caccttttgt catgaccgtt
    14821 gtatcactca tattgtctgg ttcgatgact tctagccatt gatctgccag accgccgcca
    14881 accatacttg ttgctgcttt gataagaccc ataaaaaatt cctcctctga ttcagctagt
    14941 gccaaacaat tattgtttat ttattatacc ataggaagat gtgaaacttc tcggactaga
    15001 gacggaattg aaatggaaaa ttgcgtattg tttaaatacc tgaaaaagcc gagaaggata
    15061 gtagtttttt tacctaatat cacgtataat aagagatagt gaatttttta aaaaaggggg
    15121 atccttatgg catatcaagc actttatcga gtctggcgtt cgcagcgttt cgaagacatc
    15181 gttggacaaa aagctgtgac acagacacta aaaaatgcta ttgtttccca caaaacctca
    15241 catgcctact tgtttacagg acccagagga accggaaaga cgagtgcagc aaagattttc
    15301 gcaaaagcaa tcaactgtcc gaacagtaaa gacggtgagc catgcaacga atgcgaaatg
    15361 tgtcgatcaa ttactgctgg tacgcaagaa gatgtgatcg aaatcgatgc tgccagcaat
    15421 aacggagtcg aagaaatccg atttatccgt gatcgggcaa attatgcgcc tacaaaggca
    15481 gcgtacaaag tgtacattat tgatgaggta catatgcttt caacaggagc tttcaatgcc
    15541 ttgttgaaaa cattggaaga gccaaaagaa agtgtcatat tcatcttagc aacgaccgaa
    15601 cctcacaaaa taccagctac gatcatttca cggactcagc gatttgattt taaacggatc
    15661 aatacggcag atattgtcga acatcttgaa tttatcttga aagaatcgac tatcagctat
    15721 gaaccagctg cattgaatgt gattgcccgt tctgcagaag gcgggatgcg ggatgcattg
    15781 agtattcttg atcaggcaat ctcgtttagt gaaggaacaa ttactttaga tgatgccatg
    15841 caagtgacag gtagcttaac tgacgaaatg atggacagtt acttgagtgc ttgcgtgaaa
    15901 aaagaagtac cagaagcatt ggaaacattg aaccaaattc tcgcagccgg caaagaatca
    15961 cgtcgctttc tagaggatat gttacaatac tgccgtgact tgctgatgta tcagcaagca
    16021 cctaacctag ttgaggaaca atcgagtacg atcagaagta catttacaga attggcacaa
    16081 actgtttcag cggatcagct gttttcaatg ataaagattt tgagcgatac tcaaaatgaa
    16141 attcgtttta ccaatagtgc cgctatttat ttggaagtcg ctacagtcaa acttgcacag
    16201 ctaccgtctg cttctgttcc ttcagcagat gtagcatttg cagatgatca gatagtaaaa
    16261 cagctacaac aagagattaa ggaactaaga aacgaaatca aggaactacg cagtaataca
    16321 ggaattgaga atacaaaagt acagccggta cagcaaacaa atcataaaaa acagacaagt
    16381 acctatcgtg taccgacgga acgagtatat aaagttctgc gggaagcgac aagacagcat
    16441 ctgaaccaag taaaagaagt atgggaagac ttgctgatga gtctatctgt cactcagcga
    16501 gcaatgctta aagcaagcga accagttgca gctggaccta caggactagt gattgcgttt
    16561 gattatgaga ttgtttgtca aagagcagca aatgacgaag aattgcagct ttcggtacac
    16621 aacaacttaa gccgaatgat caaagattat gcgcctgatc ctgtttatat tacaagagaa
    16681 agctggccag aacttcgaag aaattatttg acgcaggcac aagaagaggg aacagaaaac
    16741 tcttttgctt ctgaagactc agaaggagaa atcgagttgc ttcctcaaga acaagaagta
    16801 ccagaagaaa cacttgtagt aaaagcagag gaactgtttg gctccgaagc tgttacagtg
    16861 gtagatgatt aataaaaagg agtagataat tatgatgcgt ggaatgggca atatgcaagg
    16921 catgatgaaa caagtacaaa aaatgcaaaa agaaatggca gaagcacaag agctgctaaa
    16981 taaaacagag tttacaggta aagccaccaa tgaattggtc aaagcggttt ttacaggcga
    17041 tcgtcgcatg aaagatatcc aaatcaatga agaagtcgtt gatcctgatg atatcgagat
    17101 gcttcaggat cttgtcgtaa tggcagtcaa tgatgcgatc gcacaaattg atgtggaaac
    17161 tgagaaaaaa ttagggaaat acgcaaaagg tttgccagga ttttagtttt aaactgaagg
    17221 agtaatgaat atgcaatatc cagaaccaat tgcgaaactg atcgacagct acatgaaact
    17281 tccaggaatc ggtcagaaaa cagcgacaag actcgccttt tatacgatcg atatgaaaga
    17341 tgaagtagta aatgaatttg ctaaatcttt gttaagtgta aaacgcgatt tgcatttttg
    17401 tagtatctgc ggaaacatca cagaagaaga tccctgcgaa atatgtagaa atccttcacg
    17461 tgatcgaagc gttatcctag tggtcgaaga gccgaaagat gtaatggcaa tggaaaaaat
    17521 gcgagaatat cacggacttt atcatgttct ccatggtgtg ttatcaccga tggaagggac
    17581 aggtccagaa gacatcaata tttcttctct gttgcagcgg ctgcatgatg cagatgtaaa
    17641 agaagtgatt atcgcaacaa atgcaacaac agagggagaa gcaacagcta tgtatctctc
    17701 acgtctgatc aaacccgcag gtatcaaagt gactcgtcta gcacatggtc tgtctgtagg
    17761 ctctgatatc gagtatgcag atgaagttac tttattgaaa gctgtcgaag gaagacgtga
    17821 gttataaatt tctaattaag tgttttttca gatacaatat aaaagcagca caagagatgg
    17881 gggcaacaat gtgaacggac tatttattac aatcgagggg cctgatggtg caggaaaaac
    17941 cagcatcttg aatgaacttt accctctttt gaaagaagta gcaaaaacag aaatcattca
    18001 aacaagagaa ccaggaggca ttccgattgc tgaagagatc cgagcagtga tccttgatcc
    18061 aaaaaatgat cggatggatg aacgtacgga agcactgctt tatgcagcag caagaagaca
    18121 acatctagtc gaaaaggttc tgcctgctct ggcaaaaggg aaaatcgttt tatgcgatag
    18181 atttgtcgac agttctcttg cttatcaagg tgcaggccga cgcatcggcg ttaccgagat
    18241 tgctcggttg aatgagtttg ctacagaagg tactacgccg gattttaccc tttatcttga
    18301 cgtagattca gacaccggat taagaagaat caagaaaaac agacaaaatc aaatcgaccg
    18361 tttagattcg gaaggtttgg aattccacca gcgtgtacgt catgcttact taaagctggc
    18421 agaagagaat ccggaaagaa tccacaaagt tgatgcaaga aaaagctttg aagaagtgct
    18481 gcaaacaagt tatcatacaa ttattgaaca gtatccgcaa ttttttgaaa actaggaagg
    18541 tgaacgttta tgaaaattat tttagcgatt atccaagaca aagacagcaa ccgtttatca
    18601 aatgaattga ttgatgcaaa tatccgtgcc acaaaactat catcgacagg cggcttcctt
    18661 aaggctggaa acagtacgtt tatcgtgggt attgaagatg accgtgtaga agaagcattg
    18721 gaaatcatca aaaaaacgtg tgaatcaaga aagcaatttg tatctacacc tgttacactg
    18781 gatatttcga tggatggggg cgtcccttat ccagtagaag tagaagtagg cggtgccaca
    18841 gtcttcgtct tacctgttga aggattccat caattttagg aggagctgag ctatgaatca
    18901 agaaataaac ttggctcaaa tgcaaccgat cgtgtatcag cagcttcaac ggagttttga
    18961 gcatgggcgt atcgctcatg cttatctttt tgaaggggag aaaggaacag gaaaacatga
    19021 agtaggtata tggcttgcac agcacctgtt ctgtacccaa atgaaagaca atctgccttg
    19081 tggaaaatgc aacaattgtc agcggatcga aaagaaagaa catccagatg ttttggttat
    19141 tgaaccggaa ggacaaacca tcaaagtcga tcagatcaga cgtttgcaga cggaattcag
    19201 tcgcagcggt tacgaatcaa gaaaaaaagt ttttttgatc aaagaagctg aaaaaatgaa
    19261 tagcagtgcg gcaaacagtt tgttgaaatt cttggaagag ccccctggag attttttagc
    19321 ggtgttagaa acagatgcag taggaagaat tctccctact attcaatctc gttgtcaagt
    19381 tctgcatttt caagaacttt cgaaagaagc actgatccat cgattgcaag aagagcagat
    19441 tccattggaa aaagcaaaac ttttggcttt cttaacaaac agtttaggaa aagcagttga
    19501 aatctctcaa gatgaatggt ttaatgatgc taaggacttg atttatcaat ggttcgttta
    19561 cttgcaaaag caagatactc aagcctttat ctatgtccaa aaaaaattgg taaaatcatt
    19621 taaagaaaaa acacaacagt ttacaggatt atctgtcttg atgttttatt atcaggaagc
    19681 acagcgtcaa gcagtagcaa acggcgaata tggcaaagtg aaacagataa accagataat
    19741 tgaacggata ttgatcgcag agcagaaact acgcagcaat gtcagttttc aggctgttgc
    19801 ggaacagttc gttttgcata cgatttttta gcttgccgca aaggatgatg atcaaggagg
    19861 atggaaatgg tagaagtagt aggtgtccga tatagagatg caggtcatat ctattatttt
    19921 gcaccaggag actctgaata tgtttatagt gacaaggtat tagtcgaatc tcagcaatcc
    19981 ttacatattg ctactgttgt ccttcctaaa aaagagattg atcccaatga ccttcctgaa
    20041 gctatccgtc cgatcttgca taaagcgtca caagctgaat tagaaaaaga caaaaaaaac
    20101 aaaaaagatg cttatgatgc attgaaaatc gcaaaagaaa aagtgcagtt gcgtgagttg
    20161 gaaatgaaaa tggtcaaagc cgaatacaca tttgatcgaa ataaattgat tttttctttc
    20221 acagcggacg ggcgaattga ctttagagaa ttggtaaaag atctggccgc tgtatttcgt
    20281 actcgtattg agcttcgaca aattggtgtc cgcgatgaag caaagataat aggcggaatt
    20341 ggtccatgtg ggcgtccatt atgctgttct tcttttttag gcgatttcgt gcctgtatca
    20401 atcaaaatgg caaaagatca aagtttatct ttgaatccaa caaaaatttc cggtttatgc
    20461 ggacgcttga tgtgctgtct gcaatatgag agcgaagaat acgagacagc gaaaaaagaa
    20521 ctccctgact ttggaaaagt aattgctaca cctgatggta aagggaaagt cgtagggttg
    20581 aatctgctta gccgtatcgt tagtattcgg ctgactggac gggaaaatcc tgtcgaatat
    20641 gaatgggatg aattgaaaga atttatccaa gtaggtgaga tgaatggata agagatcatt
    20701 atacgacgga ttgaatcaat tagaatcaga gttgagaaat acattaacag aacttgttga
    20761 aatgaagcat gctttgcatg agatcgtaga aaaaaataca actttggaga tggaaaatca
    20821 acgtctgcgg gaacacctgc gggaattgaa ccaagttgca caagctccag ctgaaaatgt
    20881 gaagcaggaa ctttctaagt ctcgtatgaa tcttgaaaaa atttatgaag aaggattcca
    20941 tgtctgctac gatttatatg gatcaagaag ggacaatgac gagccttgtg cgttttgtct
    21001 agaagtgatc tatcgcgaaa gcggtcgatg accaataaca agaggagact ggagcggaga
    21061 aatactgctc cagtcttttt attaaggaga ttgtgacaga tgtataatca aaaaagtttc
    21121 aagggaaatc cggtatatgg ggatttatat cttgtaccga cgccaattgg aaatttagaa
    21181 gatatgagct acagaagtgt tcgtatgctg caagaatccg atcttatcgc aagtgaggat
    21241 acgcgtaata cacaaaagtt attgaatcat tttgaaatcc atacacccca aaaaagttta
    21301 catgaacata attataaaga acgtgtccct caattgattg ctttgttaaa agaaggaaaa
    21361 acgattgctc aagtcagtga cgcaggaatg ccttccatca gcgatcctgg acatgagcta
    21421 gttttagcct gcataaaaga agggatttct gtgatctcta tcccaggacc tacagctggg
    21481 atgacggctt tgattgcttc agggctagtg cctcaaccat ttttatttta tggcttcctt
    21541 ccacgtaaga aaaaagaaca gaaagaaaca ttgaaaaagc taaaagaaca gacagttacg
    21601 ctgatttttt atgagtcacc ttatcggatc actgctacat tgacgaattt cttagaaatt
    21661 tttggaaatc gccaagtcgt tttgtgtcgg gaattgacaa aggtccatga agaatacctt
    21721 agaggaagta cggaagagct actcgcttat atcgaagaac atccagtaaa gggtgaatgt
    21781 tgtctgcttg tagaagggaa tttgttcgaa atgccagtga cggaagttga tgaagagttg
    21841 ggaacgttaa aggaacaagt agaagcaaag atcgcttcag gcgaaaaacc aaatgctgct
    21901 atcaaagcag tagcgctgaa aaatgggctg aaaaaacaag aagtctaccg tcagtaccat
    21961 gaattagact gagatagtat aagttgtcta taatgaaaga ctaatgagga aatcagctac
    22021 taacttttca aatctcttga acgcgaacga atgttcttgt ataatgaata agaggtgagg
    22081 atgatggagc tgcaatttcc tttacataat gatacatcaa gaaaaatcat tcatattgat
    22141 atggatgctt ttttcgcttc ggttgaagaa cgagatcatc ctgagtttcg cggacatcca
    22201 ttagtaattg ccagacaccc cagtgacact ggtgggcgtg gagtagtgac tacagcaaat
    22261 tatgaagcaa gaaaattcgg aattcattca gcaatgagtg cacaaaaagc gtatgagctt
    22321 tgtccgcaag ccatctttaa gcctggcgac catcataaat acagcgatat ttccaaacaa
    22381 gtacgggaag tattctatcg ttatacagat attgtggaac cagttagtat cgatgaagcg
    22441 tatcttgatg tgaccgaaaa taaaatcaat tgcaagtcag ccatcaaaat cgcccgaatg
    22501 atccaagcag atatttggga gagtgtccag ctaacatgtt ctgcgggagt aagttacaat
    22561 aagtttttgg ccaaattagc atcagactat caaaaaccta gaggaatcac ggtagttgcc
    22621 cctcaagacg ctgtgccatt tttaaaagct ttgccaattg aaaaatttca tggggtaggg
    22681 aagaaaactg tacctagaat gcatgagcta gggatttata caggaaaaga cctatatgaa
    22741 tgcacagaaa tgatgttgat tcgaaatttt ggaaaaatgg gctattctct ttatcgtaaa
    22801 gtcagaggaa tccatgattc tccagtaaac gttacaagag agcgaaaatc tgtgggaaaa
    22861 gagcatactt atgggcagcc gttgcaaacg gaagaagcag tgctgactca gttgcgccaa
    22921 ctagctgaaa aagtagaaga agcactgaga agagtccaaa aacatgggaa aacagtagtt
    22981 ttgaaggtca ggtacacgga ctattctact gtgaccaaaa gagtgacatt gccagaatat
    23041 atctataaaa aagaagcact cttttatcaa gcaagcctta tatgggagga aatcttggga
    23101 gtagaaaaag gaatccgatt gttagggatt actttgacga atctggaccc gatgacttat
    23161 gaaaacatcg tccttccatt atgggaaaat caagaaatat aaaaacagct ttgagagttg
    23221 aaaccggaaa tccgttttct ctcccaaagc tgtttttata tcaagtcttg ctttttgatt
    23281 tgttcatagg tttctgttgc atccgtacat ttttcccaaa tcacaacttg attggattct
    23341 aaagattttt ggacgtcaat gatccgctgg ttgctacttc cgcgaaattg tagttttagg
    23401 tcacgtttgc ttaattcaaa gcgtccgtca acaaggatat cgatttgcga aaggagttct
    23461 aatttatctt ctgaatcttg tagcaattca tcaaaggtat agcctgtcca tgaccagata
    23521 tcttttgtcg taccgaactc ttttcggata cgtcgtacga cttgcagaca aacatctgta
    23581 ttcaaaaaag gttcgccacc aagtaaagtt aatccttgca cgtaatcgtt gcgcaagtca
    23641 gcgatgattt gttcttctaa ttcaggtgtg aatggtttgc catatcgaaa attttgtgca
    23701 gctttgttga agcagccttc gcaggcaaat aagcagccac taacgtaaag actgttgcgg
    23761 acaccttcac catcgacaaa attaaacggt ttgtagtcag caatatagtt ttggctgtag
    23821 tcttttgcta accattcttt tggttttgga tttctcataa aaaaacttct ttcatttaag
    23881 gttcaaagat atagctattg aagataaaat ctactcgacc acataaataa gaacaaactg
    23941 acctaaatcc attgttcaag aaagcttgtt cttattttga tagagggcgt tcaaagatac
    24001 aaagccatcg aaagagggtg gtgaaaaaag acttgtcata gcctcttatt ctgaataaac
    24061 ggtgttcaaa agcttgcatc tgcagtaata agcccaacta aacgaaaaat atgaggaact
    24121 atttttgttt agctattctt attacttgat gcaggacagc tttttcacaa cctcatttca
    24181 tgtgtttcac acgtgaggaa atttctttat ggcggccatg taccatcgga cgagcttgcg
    24241 gatttcctaa atagccacaa gtacgtttga ctacatcaca tgtttttgga tcatgatttc
    24301 cacattctgg acattcgaac cctcgttctg tcggtttgaa atcaccttcg aaaccacatt
    24361 gatagcaatg atcgattggc gtattcgtac caagataccc gattttgtca tatgcaaaat
    24421 cccatacagc ttctaaagct ttcggatttt gttggagcat agggtattca cagtagtgga
    24481 tgaatccacc tgaacaatat tttggataat ccttctcaaa ttccaatttt tcaaatggcg
    24541 ttgggttttt acgcacatca tagtggaaac tgtttgtata atattcttta tctgtgatat
    24601 tttcgatgat gccgaatttt tctgtatcca aacggcagaa acggtcagtt agactttcac
    24661 ttggagttga atagacactg aaatgataac catattcatc cccccatgca tctgcattag
    24721 cttttaattc tttgacgata tcaagtgtga aagcttttgc ttctgggttt gtttcccatt
    24781 cgccaccgaa gaaagctgaa gcaacttcat acaatccaat gtagcctaac gatacagtag
    24841 ctcgacggtt tttgaataat tcattgacag aatcggtacg tgaaagacgt ttcccgaaag
    24901 caccatacat atataaaatt ggcgcatttg ctggaatggc ttctttacaa cgttctacac
    24961 gatagagaag cgcatctttc atgatttcta agcggtcaga cagtaactgc cagaatttat
    25021 tctgatctcc ttttgattcg atcgcgattc ttggcaaatt caatgtaaca acacctaaat
    25081 tcattcgtcc gacatttact tcttgaccat tttcgtcttt ccacccttgc aagaatgaac
    25141 gacagcccat aggtactttg aaacttccag ttaattcaac gattttatcg taattcagaa
    25201 tatcaggata cattcttttc gttgcacatt ccaatgctag ttgtttgatg tcgtaattag
    25261 gatcttcagg gtttaagttc acaccttttt tcaaagtgaa gatcagttta gggaagatag
    25321 ctgtccgctt ttcactccct aatccattga ttcgaatctg taaaattgct ttttgaattt
    25381 ctctttcaaa ccagttttct cctagtccaa atcctaatga agtgaaagga gtttgtccat
    25441 ttgaagtaaa taaggtattg atttcatatt ccaaactttg catagcatcg aagatatctt
    25501 ttttggtttt tgcttttgca tattcttttt ggcgttctgg cgaatcgatc cattcttccg
    25561 cgtcttttag atgtttttta tagttcaatt ctgcaaaagg agcaagcaat tcatctgttc
    25621 ggtcggcaga acagccacca tattgacttg acgctacatt ggcaataatc tgtgagattt
    25681 gtgctgtggc ggtctggata gattttggcg attcgacttc ggcattgccg attttgaacc
    25741 cattgttcag catgcctttg aaatcaatca agcagcaatt cgtcatcggt gtataaggat
    25801 gataatccaa atcatggtaa tggatatcgc ctttttgatg cgcatttgct atatgtggtg
    25861 gcaacatttt cagtccgatt gattttccaa cgatcccggc tgtcagatct cgctgtgtat
    25921 tgaaaacatt actgtcttta tttgcgtttt cgttcacgac agtttggtcc ttgttgatta
    25981 gcttttcaat cgaaaagttg atatctgttg ctttactgcg agcaaaatct cgtttggtac
    26041 gataattgat gtactcttct gctaattcat attcgttttt ttctagtaat atatgttcca
    26101 cgatgttttg aatctcatag attttgacat tttcagcaaa ccggcttttg atttcttgga
    26161 caaccaagtc tgcaatcatt tgaatttttt cataagtaag tggtgataat gagccgtgaa
    26221 ttttcttttc tgctttgatc agtgcttcat tgatcttttg ttcatcgaaa tcaacatggc
    26281 ggccatcgcg tttgatgact ttcatctggg atgtctctgg ctgctgagta acgacagttt
    26341 tttcttcata atttactttc attatcttca actcctgcta aattgttctt gtctatcata
    26401 aaacaaaatc ggggaaggag caactaaata tcggctatat atagtattca atgtgctgta
    26461 taattggatt tctatactat atgtagtgta tattttattg tgaaaaaagg tgaaaacttg
    26521 tagctggtgg atttgttttt tgcatattta attttgctga ataaaataat tgaataaaat
    26581 tttttattca gtccaagtaa aagtgacaaa aacataagag caaattattc gtagatatgc
    26641 tactatgcaa ataaatagaa atgaaaaatg aaagaggagg agaagaattg gaaaagatga
    26701 tcgaagtgaa acatctaagt aaaagctacg gcagcaaact ttctcccgtc aaagccttag
    26761 ataatatctc attcacagta gaagaaggcg agtttgttgg gattatgggc ccaagtggtg
    26821 caggaaagac aacgctgatg aatatcttgt ccacgatcaa tttgcctacg atgggcagtg
    26881 tctcgatcca aggaaaagaa atcacaaaaa tgaaaaacag tgagctcagt gactttcgtc
    26941 gaaaaaaact aggattcatt tttcaagagt tcaatttgat cgatacccta aatgcaaaag
    27001 acaatatcct attgccccta gcagttgaac ggatgactaa agaggaaatg gaaaagagag
    27061 taagacatgt agctcaattg ttgaatatag aagaactgct caaacggtat cccgatgaac
    27121 tttctgtcgg acagagacaa cgaattgcag ctgctagagc acttgttgtt cagccacaag
    27181 tgatttttgc agatgaacca actggctctt tggactcgaa gtcggctaca gaattgttga
    27241 actacttgaa gaccatgaat caaaaagaaa aagcaacgat actgttagtc acccatgatc
    27301 catatacagc aagctactgc gatagaattt tatttattaa agatggtgtg attttttctg
    27361 aagttgtcag aagagggaca agaagagagt tctttgagaa agtaatagac atgcaggcga
    27421 cgattggcgg aggaggaaaa atgaatgctg tataaactag ccgttaaaag tttttttcgg
    27481 cagtcacgag gctatcttgt ttattttttc agtttgactt tatccgtaat gatctattat
    27541 tccttcagcg caatgaccta cgatcaatct ttagtcagaa gagcaagtca agatacttcg
    27601 atagatggtg gattgagttt tggtggattt atcattactg ttgttttgct ttttttcgta
    27661 ttcagtgcca accgattttt cttgaataga aggcaaaagg aaataggcat ctatcagctt
    27721 tttggtatga ataaactgca gatatcaggt atatatgtgt tagaaatcat gataattgga
    27781 ttgttcgcct gtatttctgg gatattactc ggtattattt tttccaaact cttttcaatg
    27841 attttggttc gaatgatgga tatggatctt actagtccat tttttatctc cattccatca
    27901 gtagtagata ctttatttgc ttttttcatc atccttttag cagtatctgt atattcaata
    27961 tggaaaatat ggcgctatcc aatgattcga agttttggag aaaaagaaca agcagatagt
    28021 tcaacaatgc gcttcaggac aaggcaccga ttactcgcag tcatcgggtt actactttta
    28081 acgagcggat attttggagc tagtcatttt agggaaatca cttcatggat gatcagttat
    28141 cattatcttg atacattagt tttctttttt ccttttttaa tttttttcgt ttgtgtggtc
    28201 ggtacttatt tgtttttctg ttatacattt cggttatttt tgatcatatt gagtaaatgg
    28261 aagatttatt actatggtat caattttttg ataatgggta acacgcaagt tcatttgctc
    28321 aaaagttggc gtacaaattc tctgattaca gtcattctcg gaatttcttt aatgacaatt
    28381 ggcggcacgg ctagtatctc tactatttta tcctaccagg agaaagtttc ctccccgatg
    28441 gactatcagt ttgatgttga gacagagaaa aaaatcaagc cgattctagc agaggaaggg
    28501 cagaagataa ctaaagaaat caccttgcat tataaagtca ttgggagtat ctatagtcaa
    28561 aatctcgaag aacctatgtt tcaaactgct aatctgatca cggaaacaca atatcaagcc
    28621 ttcagaaaag ttaatccaga tttgccgaac atttctttga aatcacctgg tcatactgtt
    28681 ttgcttgatc aaatgcaaac tctttttcgg aatatttcat tatatggatc tagtattgga
    28741 ctgccagata atcaagaatt gaagattcaa caaatgcttc caagtgtatt aggtgacgaa
    28801 acaatggtat atctaagtcc tgtactgatt gtagatgata ccgtttttga aaaagcaaaa
    28861 ggtgtggatt atcaagtagt caatgtagat gtatcaggtg gaaataatga aaaaatagat
    28921 gaacgactga gcaatgacct gtcgataaag tggctcaatg ctgtttatta ttcttatgat
    28981 atcgtaaatg gaagtctaca aggagaaatt agcacaaaga aacttccgga tgaccaaatg
    29041 aaggaagcac aatttagcca agaaagctct cgtttgaatt tctcttccag ctattcgaaa
    29101 ttgcgtgcag ttaatcggcg aatcggtatc aatatttttg tcacactatt tgtcgggatg
    29161 atcgtgatta ttgctactgg tagtattttg acagtccggc aattagcaga agcagaggaa
    29221 gaaaaagaaa attatcacct attgactaag ctggggattc cacaaagaag gatcagacgg
    29281 atgatcttcg agcaaaacgc acttaccttc tttccaccca tgatattagg gatcactcac
    29341 gcagtatttg caatcaatgt cttcactcag tatataaaaa ctgccgatta ctggttggct
    29401 tatcttgtga gtggcttatt gatccttatc tatcttttgc tttatgtcat tacgagtcag
    29461 ttatattgtc ggattatcga ggaatgatat acggaataag acaagataat aaatagaact
    29521 aaattttgaa taccatttat ttggaataag aggttgtgac acgatttttg tcacaacctc
    29581 tttatttttt atatttttta aaagtacgag aaattataaa gaaaaaagca gtgatttgat
    29641 gtgctatttt tataaaagct atgtgacaaa actgttagta aaaaaacggt ttctgtaagc
    29701 tagacacatg tttctttttg tcaaaattag ttacactttg tgtgaggtta tatgaggagg
    29761 gaatccaatg aacaaatggc gttctaatcc aaaattgaat tattggggaa aattcatcat
    29821 tcgaacaatt ttttatagtg cagtactgct gttcctgatt tatctgtatc actataaaaa
    29881 tattcaagga ggaactttta tctataacga attttaggag gaagagtatg gaaatcaaaa
    29941 cgattattga agcaatcgat gattggggaa taaatgaacc tgatcgagtt gcttatcagg
    30001 cggatgagac gcatactttt ggtgaattga aacaagcatc agacgcactt gcttattatt
    30061 tagaaaaaaa ggtagaaggc gatggaccaa ttgtcgtttt tggcaatttg gagtttgaaa
    30121 tgattgtctc atttctaggg gttgtcaaat ctggccatgc ctatttgccg attgaagagc
    30181 atacacccaa ggagcgtatc ctatcgatct ttcgggtggc taaaccatct atggtcatca
    30241 gtatcgggga ctggatagaa gatattcctt cggttcctgt cataacaaaa aaagaatttc
    30301 aagaaatcaa gaagatacca gtaggatttt atcccagaaa tagcgtaaaa ggtacagaaa
    30361 atttttatat tatttttact tctggtacta ctggagaacc aaaaggcgta caaatcagtc
    30421 atgataatct agtcagcttt gttcaatgga cgatggaaga ctttgggatc aagcctggta
    30481 tgcatttctt agcccaagcg ccattttctt ttgatttatc ggtattttct atttatcctg
    30541 cattagtttc tggcggaatg ctgaagccgt taaataaagc agttgttcaa gactttcgtc
    30601 aattattcgc gacattgcca gccttgaagt tgaatgtgtg ggtttctact ccatcattta
    30661 tggatatttg cttgatggaa cctacattca atggagaaaa tgtgccggaa ttggacatgt
    30721 ttttattttg cggagaagaa ttgacgaaaa aaactgcaga aagtctactt gatcgattcc
    30781 caaatgcacg gatatataat acttacggtc caacagaagc aactgtagct atttcaagta
    30841 ttcagattga ccagcatgta ctagatagtt atgaccgact accgattggc tacgtaaaaa
    30901 aagacacgaa agtttctatt gtccaagaag gtaaacctgt ttcaaaagga gaaacgggtg
    30961 aaatcattat tgcaggacca agcgtatcaa aaggttacct gaacaatccc gaaaagacag
    31021 ctgctgcgtt cttttgtcaa aatgacttag caagttacca tacaggggat gcaggtagac
    31081 tagatgaata tgggctttta ttttatgaag gacgtatgga tcagcaggtc aaattacatg
    31141 gataccggat tgaactcggg gatatcgaac attatctgct ccaagacaac cgaatcaaac
    31201 aagctgtcgt cgtaccaaaa taccaaggaa caaaagtaca gcagctggtt gcttttgtcg
    31261 tattagaaaa caaaacgaaa cagccggatt ttcagttgac taaatctatc aaagaacaat
    31321 tactagaagt tgtaatggat tatatgattc ctcagaagtt ccattatgtc gatctgctgc
    31381 ctcagacagt taatggaaaa atcgatcgaa aaaaattaac agctgaggtg aatcctgaat
    31441 gatgttacct catatgatcc cttatgcaga gccgttttat ttttttcttt tagccattgc
    31501 gcttgtaccg atcgttctat ctttattgat atgggaaaaa agattaccag tttatcaatc
    31561 agcagtgaca cttttctttt tatttgtcag ctttggaggt aattattggc aacagggagc
    31621 ggcgctgatt gggtatgttt tgtggcagac gatacttgta tggggctatt ttgtttatcg
    31681 gcaaaggaaa aatcagacag gatggttttt tgccgccgtt attttagcga taattccact
    31741 gtttttaacg aaagtgactc ctttttttga acatggaaaa gcatcgttac ttggtttttt
    31801 aggaatctct tatttaacct ttaaatctgt acaaatgatc atggaaataa gagatggcat
    31861 gatcaaaaca tataagccat ttcgttatat ccagtttctg ctgttctttc caacaatctc
    31921 ttctggacca atcgatcgct ataggagatt tgaaaaagat cagctccatc cgcctgttcg
    31981 tgataaatat gttgatcttt tgggaaaagg cgtccaaaat atttttgtcg gttttttata
    32041 taaattcatt atcggttatt attttggtca agtactgctt cctgtagtgg gacgcattgc
    32101 cttacagcat ggtggtattt catgggcatt agtaggttat atgtacgtgt acagtatgta
    32161 tttgtttttt gattttgctg gctatagctt atttgcagtc ggtacaagct acatgatggg
    32221 atatcagaca ccaatcaatt tcaataaacc ttttttatca tggaatatca aagaattctg
    32281 gaatcgttgg catatgacgc tgtccttttg gttccgtgat tatgtatata tgcgattgat
    32341 gttcttcatg atgaagaaaa aattttttaa aagcaagatc gtaatgtcaa atatcggtta
    32401 ttttgctttg tttcttctaa tgggtatatg gcatggattg acgtggtact acatcgtata
    32461 tggactgtat catgcttgtc tgatctgtct gacagatgca tggatccgat ttaagaagaa
    32521 gcataaagag cagttgcctt ctaacaaatt tacgcatggc ttagccgtat ttttgacatt
    32581 tcaagcagta tgttttagct tcctgatttt ttcaggattt ttagataaac ttttttttag
    32641 ctaacgaata gtgacatatt aatttatata acaaaggaga aaaggaaaat ggaagaacaa
    32701 attttatcaa tcttagaaga aataacagga acagatgaag taagaaaaaa tcgtgatatt
    32761 gatctatttg atgaagggtt gcttgattct ttagcatctg ttcaattatt agtcgagcta
    32821 gacggagagc tggatatcca agtccctgta tctgaattcg agcgagagga ttggtgcaca
    32881 ccgaataaaa tcattcaaca agcaactgca ttgaagggtt gagtatgaaa aagaaacttt
    32941 ttgctatttt cggcccggtt gttttagctg ttggcttact gtttgtgttt tttacctcct
    33001 cctatcaagt agatatctct aatccagaaa cgattagacg tgcctcttct tccatgtcag
    33061 tgaacgtgtt gaaaggagat gcgataaaaa attatgcatt atctgaaaaa caatatattc
    33121 ctttttttgg ctcaagtgaa ttaagcagaa tcagtccatt tcatccgtct gttctggcag
    33181 aaaaatacca gcgaaactat cgtccatttt tgctgggagc acccgggaca caatcgttaa
    33241 gccaatatat gatgatgcgt tctgcaggag atgctatgaa aaacaagaaa gtcgtcttta
    33301 ttatttctcc tcagtggttc gttaaaaatg gtgtaaaaac tgattacttt aatacgtatt
    33361 attctgaact tcaaacatac gactggttgt tttcaatgaa gaaagtaacg cctgctgatc
    33421 gctatttagc tagaagatta ttaactttct caaaagtaaa agagaatgat acgttgacgg
    33481 cgattctgca aacgatcaaa aaagggaagc tacctttacc tgaatctttg aatcagctgc
    33541 gcagccaatg gaacatgctg aagagagagg atgaagtatt cagcaatatt gggttgaaag
    33601 atcgacagca aaaaatcgat catgagtcaa aacgattacc aaaacaatac caagaaacag
    33661 aattatccat tttagcaaat caaattggag aacgagaaac gacgaataat ccattcgggt
    33721 tgaaaaacga tttttatacg catcgtatcc gagcccacga gccagagctc aaacagtcac
    33781 aaaaaaattg ggattatcgt ttttcgccag aattttctga ttttcagctg gtcttggatc
    33841 aattagcaaa aaaccataac gaagtattgt tcatcatccc accggttaac gagaaatgga
    33901 gcgattatac cggactatct caagaaatgt tacaaggatt tgccaaaaag atcaaatttc
    33961 aattaaatag ccaaggattc aatcgaatag ctgattttgt caatcaagcg ggtaccaact
    34021 attttatgga agacacgatc catctaggct ggaaaggctg gttggctgca gatcaacaga
    34081 tcagaccgtt tttagaagaa aatcatatta ctgctagtaa gtatcatctt gacgatgctt
    34141 tttttagcaa atcttggcaa catcaaatac cagataaact acaattaaaa taaagaaaac
    34201 aggcgagtgt tgctcgcttg ttttctttat ttttgaatat tctgaataaa agataaaaat
    34261 accaagtttg cctgaacaaa aatcataagc aatggtataa tcacaatatg aatatagaaa
    34321 tgggggaaaa catttggata caaaagaaga acagctactt attgatttaa cttctgcaaa
    34381 aggagtacct ggaaatgaag aagaggtaag agaagtattc agagaatatg ccaaaccatt
    34441 cgctgatgat attttttatg acggtttagg cagtgtgatt gccaaacatg gtgctggcgg
    34501 aggccccaag gtctttattt ctggacatat ggacgaagtt ggttttatgg taacaaaaat
    34561 aaccgaaaaa ggattcttgg aattccaaac tcttggaggt tggtgggggc aagtcatgct
    34621 tgctcagcaa gttgaaatca aaacacgtga agggaaaatc gtccacggtg tgattggttc
    34681 caaaccacca catgttttaa cacctcaagt aagaaataaa ccttatgaaa taaaggatat
    34741 gttcattgat attggcgcat caagccaaga agaagccaaa gagtggggca tacgtcctgg
    34801 agatatggtc actccatata ttgaatacaa acgaatgaat ggcagtaaat atttactggc
    34861 aaaagcatgg gataatcgta tcgggacagc tgtttctctg cgagtattag agaatctatc
    34921 taaagaagca cacccaaatg ttttattcgc aggaagcgat gtgcaagaag aagtaggctt
    34981 gcgaggtgct cgaacaagta cacaccttgt aaatcccgat attgcttttg ctttagatac
    35041 tggtacagca ggagacactc caggaatgac accaaaagaa gcagattcga ttttaggaaa
    35101 aggcccacag attttgattt ttgatgcatc tatgatcccg cataaaaaat tattgaattt
    35161 tgttattgat gtagcagagg aaatggatat tccattccaa tatacagtga tttctggcgg
    35221 aggaactgat gcaggacaaa tgcatttgac aagagatggt gttccgtctg tagccattac
    35281 cgttcctgtt cgttatctgc attcccatac atcagtgatt catgaagatg attatttgaa
    35341 tacagtgaaa ctagtcacag aattagtgaa acggcttgat agtgaaacag ttgaaaaaat
    35401 tcggagttat gaataatgaa taatagaata cctatttctg aatatcggaa aaaagcacga
    35461 gtttctttgg aaaatcaatg gggaatcaat acttggcttg ttttcttatc tataatattt
    35521 acgagcgtca tccagaactt ttttgacggt ttcagtcctt ttccggaaga aagcatgcag
    35581 cagcacttat tgagttttct agtcgataat ttacttttat tcgcattcaa ctatgcattg
    35641 ttttacattg cgctagtagt tgtacggggt ggcagaccaa aaagtaacct tttattttca
    35701 gttttccaaa gaaagtattt tggttcgata cttggcatca atttgatcaa ttcagtcatc
    35761 aactggctac tcaacagttt aatctttctg cccatgtttt taatcagcgg agtgagtact
    35821 tatatgcagc tgcttttcag ttcaaatgcg gtacctacgg gtgtcatgcc agaagatttt
    35881 cttgatatca gctttgtttt atcgatgata gggatgattt ttgtttcagc actcatcatg
    35941 agtattgttg gcggtttatt ccaatttgct gcatggacaa agttcgacta tcctgatttg
    36001 tcggttattc aatgtttgaa atatggctgg tttttattga aagatagaat tggtacgtat
    36061 attttgttgc agctgtcttt tatcggctgg tatttgctag gagcaatcgc tttgttcatc
    36121 ggtcttttgt gggtcattgt ttatgtgaat gtgtcagttg cagaatttta tgaacaagca
    36181 cggattgaaa aaggaggtcc agacgcctat tttacttagc atgtaagcag tttattcatc
    36241 aaaaaaataa cttttgtggt aggataaatc tttgaggggg aatgtacgat gaaattaact
    36301 gttttaggat gcttaggagc gtatccttat aaaaatcaag ggacaacagg ttatttgctt
    36361 caatctgacg ggttcaattt gttgatcgat gcaggtagtg ctacgctgat caaattacaa
    36421 gaacaccttg atccacttga tctagatgct gtgatcctta gccattatca tcatgatcat
    36481 attgctgacc ttggtgtatt acagtattac tggcaacttc atcctaagag aaatccggag
    36541 acattgcttt caatttatgg acacacaaaa gattccattc actttgaaga attgacgttg
    36601 gaaaatgtct caaaaggaca tccttatttt gaagctgaag aattaaaaat tggtcctttt
    36661 ctgattactt ttatggaaac aatccatcca gtagtatgtt acgccatgcg tatcgtagaa
    36721 gaaacaacgg ggaaagtatt cgtatttaca ggtgattcag gttatttgga aagtttcgct
    36781 gattttgcga aagatgctga tttgtttcta gcagatacct atctttttga aggaaatgaa
    36841 catcatcatg cccatttcac ttcaaaagaa gctggagaac ttgcgaaatc agctaacgta
    36901 aagaaacttg tacttacaca cctacctcag tttggtgatt tagaacaatt gaaaaatgaa
    36961 gcgcaaaagg cagcaggcaa agggattgat atagaacttg ctgcagttga caaagtgttt
    37021 aacatttagg aggaagaaca tgatttttgt accaaatgaa aaccacgacc caagaatcaa
    37081 tttagcaatt gaacaatatt tgctgcaaga aatgccttta gatgaaccta ttttgctctt
    37141 ttatatcaat gaaccttcaa tcatcatcgg cagaaaccaa aatacgcttg aagaaatcaa
    37201 tcgagattat gtggaagaaa atggcattca tgttgtccgt cgcttaagtg gaggcggagc
    37261 tgtctaccat gattttggaa acctgaactt tagttttatc atgcctgatg atggggattc
    37321 attccgtgac tttgcgaaag taacacagcc aatcatccaa gcacttcatg aattgggtgt
    37381 tgctggggcg gaattgaaag gccgaaatga tctagtcatt gatggcatga aattttctgg
    37441 caacgcgatg tatgctacca acggtcggat gtttgctcat ggaacaatta tgttcgactc
    37501 tgatatcaat gaagtagtca atgcattaaa agtaaaaaaa gacaaaatcg aatcaaaagg
    37561 aatcaaatcg atccgttctc gtgtaacgaa tatcaaacca tttcttccag aagaaaagca
    37621 aaaaatgaca acagaagaat ttcgtcaaga aatcttgttg aaaattttcg gcgtagagtc
    37681 agtagaagaa gtaaaaacgt acgaattgac tgatgaagat tggaaaaaaa tcaatcgaat
    37741 ctctgaggaa tattatcgaa attgggattg gaattacggg aagtctccag atttcaattt
    37801 ctctcgccaa aaacgttttt ctatcggttc gattgaagtc catttgaatg tctcagaagg
    37861 aatgatcaaa gaagcaaaaa tctttggcga tttcttcgga ctaggagata tctctgacgt
    37921 ggaaaaaaaa ttggttggac agaaatatga taaagcatct ctatcaaaaa tagtcagcca
    37981 aattgatgtg aagaaatatt ttggtgcgat agaaccagaa gaattgctta ccttacttta
    38041 ttgaaaataa aaaatagcgg aatccacata tatggattcc gctatttttt atacaggatt
    38101 cgggtaattt cgagagagtt gaacagtgat ccgatcaatg aaagtatctt tttcctgcgt
    38161 tagtgttcct gtgtaacgat ccgctacgtc tttcatcatc attaagtcaa aacgttcctg
    38221 atcatctaat tcttctgtga tgcctcgact aaataggttt gtatcgtaat gtgcactatg
    38281 ttcgcaacaa atgacgaaat gttcctcttg ttcgtgtatc gacaaataaa tttttcgata
    38341 cagtggattt tcgatggcat gagtttctct aaaggaatgc tctaaagcgt ggatcaaaag
    38401 ttgagccaga tcgtcatcgt tgataggaag ttcttcggga agatctgctt ggaaggttat
    38461 ctgaatattc cgtttggcag ctaacttctg gtaacgcgct aagataagat ttgtcacctg
    38521 gtggttgcaa aaagaacaca gcatttcgaa attttttgct tcttctaata tcttttccac
    38581 atagttttta gcttttgttt ggtccgtatg cattaaatct tgcagattct ccatctcttg
    38641 gacaaattcc aggcgaagtg tatttacttc ttggatatgc tgtcgaagct gctcataatg
    38701 ttgctccaac aaatcatttt tgatttttaa gaaatctact tgtctccgat ctttgtcggt
    38761 atttaagatg tgatcgatta gattgtagct gataataacg attagaatga taatatataa
    38821 aatcgtagac caattgatcg ttgtttgata gatacctaat gccgtttgga tgtaaatcaa
    38881 gcaatggata atggctacca agacgatcca aggtgtacaa gctacataaa aacggttatt
    38941 cttatatgct tcgccaatgc atgcaagtga agtgactaaa gtgcttataa cactagcaat
    39001 attagcaatc ggcattaatt caggaagttc tgcaggagta aaagttgcat aaagcagaac
    39061 gccgataaaa atcaaaagtt ggaacgtaca aaaaattccg taccattttt tatagtaaaa
    39121 cattcggctc caatagtagc agatgatgaa agctgacgtt aagataataa ataaatgaga
    39181 taaaaaagaa tgaacgaatg gttcaaaaag aataccactt aaaatatctt ctgatacagc
    39241 ttcaaatcct aatgccaaag caaagcaact taagagaata agtgagtatt caagacgttt
    39301 ttgtttatac atcaagtaga tcgtatagaa aaaggtccct atagcgataa aaacagcgat
    39361 tatcatcgtc aatacttgcg gcactgattg tgaataaata aaactaatca atggtccagt
    39421 tgttcctaga taaactttcg gtggcagtcc agcatagttc tgataaggag atgagactgt
    39481 aagggtcaat ttttttccga tataatttgc tggtaaatct atcacagcca atgagagtcc
    39541 gggattgctt tcttcctgat aatctttccg ttggtataaa atcttttcgc ctagatatac
    39601 agtaatccat tgatggtttc ctttgattag taaatttgct tcgtccaacg gtttttcaag
    39661 tgtcgtttcc ataatgaatg tttcgtcttt atccacggta ggaatcgtac gagtgtactt
    39721 gctgtcaaac ttaaaatcag gatcatcttt tgtatagaaa acccacgaat cttttatttc
    39781 tttcatttga ttgctgtccg caggagaaaa aataaaataa aagaaataag caattcctgt
    39841 taa
//
LOCUS       DAYYME010000007        39084 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000007 DAYYME010000000
VERSION     DAYYME010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.007"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            10..231
                     /locus_tag="ACHBFW_000288"
     CDS             10..231
                     /locus_tag="ACHBFW_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9316636.1"
                     /translation="MSISYNKLWKLLIDLNMNKTQLREISGVSPNVIAKLGKNELVAM
                     ESLLKIAAALNVDVSEIISSDVIDAEDVK"
     gene            232..2949
                     /locus_tag="ACHBFW_000289"
     CDS             232..2949
                     /locus_tag="ACHBFW_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFM9316637.1"
                     /translation="MAKNKVLNITEIEDGVKKILSNRGDDFITQFLSLYDIPKTSITR
                     AKSKFDNGEPFVIKNKLHYEEIEGNVVIAIDAIAQSIKEQKSKPRYILVNDYLDIAAI
                     DTKTHETLNIPIEELPANADFFLAWNGIEKADYQAENPADRKAAERFAKLYDVVAKDN
                     PDANEHAFNLFLIRVLFLLFAEDTGIMNKGSFTNVLKTRTAEDGSNFNEVIKELFAIL
                     DINELARNGKDEWLLEFPYVNGKLFGEPHVDLTFSKVSRELLIEAGELLNWNEINPDI
                     LGAMIQSVASAEDRHVAGMHYTSVPNIMKVIKPLFLDDLTESFETLKLRSEENELKDI
                     TEKTRNDNKRTIINDLSALLTRISNIKFLDPASGSGNFLIIAYKEIRRLEIKILQLLG
                     DLQNNDSMPLSVIHLGNFNGIELDDFAHEVARLSLWIAEHQMNKELEEAIPGTIAELL
                     PLKDAGNIVCANSLRIDWNEVLRLKENHEIYIMGNPPYLGKSLQNAEQREDMEKLVGD
                     IRKYKLLDYIFGWFWKAKQYIATSHGSFAFVTTNSICQGEQVSILWPYLLKDEVRISF
                     AYQSFKWNNNAKNNAGVTVVIIGMTNNVNIHPKLYLENGSVMSVSNINAYLANGENVF
                     VNESRNSISNLPSLKFGSMANDGGNLVFSVAEYWNLIQKYPDIKHLFKKYLGSTGLIN
                     GDLRYTLWLTEEDATRYKQNSLIKETIKNVKSYREKSKRKETRALAEKPWSFGEVRYD
                     ETSENSILIPRVSSEKRDYVPMGIVGSDTIISDSAMAIYNAPMWLLGLLESRMHMVWL
                     RSVGGKLETRYRYSAGLVYNTFPIQDLSAQRKNEMTRVMTEILDLREYEGGTLADLYN
                     KDSMPESLRKKHQELDGIVDRAYQQRPFESDEDRLGTLLKLYQEMTIND"
     gene            2942..4897
                     /locus_tag="ACHBFW_000290"
     CDS             2942..4897
                     /locus_tag="ACHBFW_000290"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM9316638.1"
                     /translation="MTSNLFEINFDKNQSKTTNDLGMREMQERVYEKRASQYLLVKSP
                     PASGKSRALMFVGLDKLHNQGIKKVVIAVPERSIGKSFKDTELSKFGFYWDWEVKPSD
                     NLTIGGGEKSKVQHFVKFMNSTDPSDNILIATHATLRFAFEELDDSLFDNTLLAIDEF
                     HHVSRDDKSVLGNALRSIMSNSNAHILAMTGSYFRGDSVQILEPADEDKFDKVTYTYY
                     EQLEGYKYLKSFAMGYSFYRGKYTDALDKVIDVNKKSIIHIPNVNSGESTKDKYAEVD
                     EILDLLTDGGNITQNEDGIYEIERPDGKKLLVADLVNEEGREKVSAYLASITDDIKDL
                     DKLDIIIALGMAKEGFDWPFAEYALTIGYRNSLTEVIQIIGRVTRDSPNKTHAQFTNL
                     IAQPDAQDDEVLFAVNSIMKAISASLLMEQVLAPVYKFKPKDGKPDKPGDLSIRGLKP
                     ADTTERTKKIIENDMEDLKSSILQSQDIQNAIVSGADAKMINKTLIPRVIIDRYPDLT
                     NEELETVRQHTVADINIAAGRTTTSEDGSREIIKMADRFINIDELNIDLIDQVNPFQR
                     AYEVISRDIDAPTLRFIQDYLSSNKYDFTDEQLVAAYQAAKKFRAENGRNPDRNSKDD
                     GEKYLAFALLRLAEIKREREAAKEDGE"
     gene            4887..6023
                     /locus_tag="ACHBFW_000291"
     CDS             4887..6023
                     /locus_tag="ACHBFW_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM9316639.1"
                     /translation="MANSLDSIFEDDAFDDLTKQVEKKKVVKQDPEVAKFQEIIDFVN
                     ENGREPEKTPEWSSERALWARLSGFRGKPERSEKVKKYDTLGLLEKEFEPDGEFLADS
                     VEIDDELNLDDILGSDDMIDDFSDLLDVSRYRRTVNAADKIGRRKHASHFEKYKSLFD
                     QVHADIASGRRKIIPFEQYDIHENRFYIQNGVMMYIVSISKDTFIADNGKTNARMHVV
                     YENGTENKNLLLQSLASSLYSTERHGRMVTEPVDEETLAESFGEEFTTGYIYVLKSLS
                     TNPDISKWNNLYKIGFTRNDVESRIANAENDVTYLNAPVKVMLSAEVQNVNAQLLERT
                     LHHVFQDKQVVFQDGNFKKATEWYMVPLNDIESKINEVIAGLQK"
     gene            complement(6074..6277)
                     /locus_tag="ACHBFW_000292"
     CDS             complement(6074..6277)
                     /locus_tag="ACHBFW_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9316640.1"
                     /translation="MKFSYNPLWKLLIDRGLNKAQLRKMTDISSATLSKLNKGENVTT
                     DILLRICEALDCDLSDIVEIVRD"
     gene            complement(6293..8125)
                     /locus_tag="ACHBFW_000293"
     CDS             complement(6293..8125)
                     /locus_tag="ACHBFW_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9316641.1"
                     /translation="MQNYYIERVTIKGKDREDATVKFTNGLNIISGPSNTGKTSIVTA
                     IDFLYGAKKDAAPFSTDATGYSKVELIIQSTNGKYSLTREFDSNSILVSKITSSGEYV
                     DYEKYNARSGKNPISHFWLQLLGIDDDYQIIKNEDFKRQKLTWRTLAPSTLIPQDRVN
                     TSASVLFPGETTQRTAFLSSLLLLLYGSDFANSEENETKEHKKIRRNSVINYINSSLS
                     ELYENYEVAKSELPEAPSDDFQQIVDGMFAELETIEKQINDALASSKILIKEIYQKEQ
                     YLHETQLLLNRYKQLHYQYQSDIERLTLIVDGERLLNDHNEHDTSHSCPFCNGELSKP
                     VQTTYIDTARSELHSLLSQLSDLTEESHDTEVELQELMEQLDELKTRKKSLDAAINDE
                     LKPKAETLKDRINKIETYRDIKQQLISMEKMQNKLKSDLEKIENEPEDERILYKPKEK
                     FDAEFYKEMSELLDSMLRNMHYKPNEFRNAFFAKDIFDIKINGNLKWTENGHGYTAFI
                     NSVVVMAYRHLLYNHAKYTPSVFIIDSPLLGLDERKKQDGDDNMKEGLFKYFMDHQNE
                     GQLIIVENTTNLPALNYDDKQVNQIVFTEDKNNGRYGYLYDIYN"
     gene            complement(8128..8574)
                     /locus_tag="ACHBFW_000294"
     CDS             complement(8128..8574)
                     /locus_tag="ACHBFW_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system middle component 2"
                     /protein_id="HFM9316642.1"
                     /translation="MNSLFNTPFEISVRVMLLLSEFKEPVSADRILITDFITTYGRDF
                     EISDYNLNGDNSYRFSEFIARRELVKDAVKNLVLQHVIQPSQSKEGFKYALSEDGLKL
                     ISQFVSVYAEKYLQIADESVKYIQDKSDVELLRQINQRAKEFKGEQ"
     gene            complement(8567..9517)
                     /locus_tag="ACHBFW_000295"
     CDS             complement(8567..9517)
                     /locus_tag="ACHBFW_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system protein"
                     /protein_id="HFM9316643.1"
                     /translation="MEISEITQTLYPFMGSEYVKPQDFLIALLDSVSNDDEATIFGFS
                     DDNLRRFYNGTRPLTQEYAVRVAGKFNIDKCTTFIYERLIDDAPTVLKQQLIDKGVVI
                     PKDTDVSEIVAHLLLDEITSIASQTKVKTKKAKPKLSELELASLRINTNGDFSYNDEV
                     KKFFDDLPVPLDIRAEEMTYVIALFSAYADADGYEVYEHTNDLPNSRLKDFKRQRRNY
                     YKAESIRRGVRDNFTQEEGTEHFEALKEDMYDGIEEVYEEDYDDGVERLKAVLQQSSV
                     ITLNGSILSFIPGLLQNSAKKGICHMLVNDGKIKWVTEDE"
     gene            9912..10529
                     /locus_tag="ACHBFW_000296"
     CDS             9912..10529
                     /locus_tag="ACHBFW_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316644.1"
                     /translation="MSRFMKTHQNDRSTYKYVFERTDANGRIQKEVVEIRPGEDGVTE
                     LNIQELHRADDRAIYNFYKNARPERTEEEKEKIKAWKEKFREDFNNEHGYYPMDEYVD
                     GEANDYFKRNYNLSLNEFDDECDEGDNPIQSLVYDAYQDNEEDPRIDRLREVVTQLLP
                     YHQEIYHMLVTENMSQTEVADKLDKKKQSINRTFGIIKDKIKELF"
     gene            10626..10832
                     /locus_tag="ACHBFW_000297"
     CDS             10626..10832
                     /locus_tag="ACHBFW_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316645.1"
                     /translation="MNLKHKIRVNVTDDTGNKETVLQGGVRHLPRWFARWLFGKGNMD
                     KILVLTPGDTIESVEIREVNKKGA"
     gene            10837..11151
                     /locus_tag="ACHBFW_000298"
     CDS             10837..11151
                     /locus_tag="ACHBFW_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA biogenesis protein rrp5"
                     /protein_id="HFM9316646.1"
                     /translation="MSKTKLFLQVVEDVQALADSLQELAVAMKTNEPEPQVETPEPTM
                     ETKSEVKQPTLEEVRGLLARKSQEGKSTEVKALIEKYGASRLSDVPTENYAALVADAE
                     GL"
     gene            11152..12294
                     /locus_tag="ACHBFW_000299"
     CDS             11152..12294
                     /locus_tag="ACHBFW_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFM9316647.1"
                     /translation="MPTQHAVLSASSSNRWIHCPPSVRLSEHFENKSSPYAQQGTDAH
                     TLCEYKLNKFIGNDVTDPTSTLEFYDEEMEQCAESYATFVMEEVAKAKQTTVDSIVIV
                     EQRLDFSRYVPDGFGTGDCLVIADGTLSVIDMKYGLGILVDAYQNPQMMCYALGALEL
                     FDVIYDIQEVKMTIFQPRRENVSTYTLLKEELLQWAKDVLSPAADLAFKGEGEYQCGK
                     WCGFCPAKNSCRARADHNLKLAQYEFKPPELLSDDEIEEIIGKVDDLVSWSNDIKDCA
                     LKLALGGKQWTHHKLVEGRSTRKYSNDNDVAAAVIKAGYDPYDKKLLGVTAMTKALGK
                     EKFNEILGEYIIKPKGKLTLVDGSDKRQAVTVNNLNEEFKPLTEEQ"
     gene            12296..12838
                     /locus_tag="ACHBFW_000300"
     CDS             12296..12838
                     /locus_tag="ACHBFW_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFM9316648.1"
                     /translation="MSKTSKVITGTNTRLSYFHGWEPASINGGAEKYSTAVLIPKSDK
                     RTIDTIEKAIDAAIEEGLAKFGGKKPNKAEIKLPLRDGDIERDDEAYQGHYFINANST
                     TAPQIVDQHVQPILDRSQVYSGCYARVSINFYAFNANGNRGVAAGLGNIQFVRDGEPL
                     GSQVSASDEFETLADDDFLA"
     gene            12899..14836
                     /locus_tag="ACHBFW_000301"
     CDS             12899..14836
                     /locus_tag="ACHBFW_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFM9316649.1"
                     /translation="MKHLSLDLETYSSVDLSKCGVYKYSESPDFEILLFAYSVDGGPV
                     EVVDLKMDETIPAEILTSLSDETVIKHAFNASFERVCLSRFLGLDGFLNPRGWQCTMV
                     WGATLGLPLSLVGVGAVLGLDKQKLTEGKDLIRYFCVPCNPTKVNGGRTRNLPHHAPT
                     KWSAFKTYNSRDVEVEIAIQGKLSNFPVLDSVWKEYWLDQEINDRGIEIDKDFVEAAV
                     QLDELSKAGLNQQLQELTGLENPNSVLQMRGWLLDHGLETESLGKKAVAELLEQPIDE
                     NLKQVLVLRQQLAKSSVKKYQAMQNFACSDNRSRGLFQFYGANRTGRFAGRGIQLQNL
                     PQNHMSDLSEARSLVKQSNFEMLNILYGNVPNVLSELLRTAFVPREGMKFIVSDFSAI
                     EARVIAWLSGEKWRQEVFKNGGDIYCASASQMFGVPVEKHGVNGHLRQKGKIAELALG
                     YGGSVGALKAMDALEMGLTEDELQPLVNSWRDSNINITELWWAVDKAVKTCVKERRST
                     ATHGIKFEYKSGFLFIQLLSGRKLVYVKPKIGENKFGGEAVTYEGVGATKKWERLESY
                     GPKFVENIVQAIARDILCFAMENLRKYRIVGHVHDEVIIEVPMESKVEDICQLMSQTP
                     KWAEGLLLNADGYECEFYQKD"
     gene            15047..15817
                     /locus_tag="ACHBFW_000302"
     CDS             15047..15817
                     /locus_tag="ACHBFW_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFM9316650.1"
                     /translation="MNELKVFENAEFGSVRTVMIGDVPHFVGKDVASILGYTNPQKAL
                     RDHVDEEDKTVNESFSVNGTMAILINESGLYSLIISSKMPNAKKFKRWVTNEVLPSIR
                     QHGVYATEDFITKSIEDPAWAISVLKQLQEKKEMVAMQQQMILEMKPKVSYYDLILQN
                     NSVMSISKISKDYGMSPQAMNKLLHELGIQYKQGKMWLLYQKYANQGYTQSKTHAIDA
                     ERSVMHTYWTQKGRLFLYDLLKSKKNILPVIERGDNNA"
     gene            15810..16154
                     /locus_tag="ACHBFW_000303"
     CDS             15810..16154
                     /locus_tag="ACHBFW_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFM9316651.1"
                     /translation="MPRRKDYRTSVYVCSPFSGNVVVNHKNTKRYCRIALDRGYMPVA
                     AHLMYPQFMDDATERDVAMHMSLVLMGKCEEVWVVGDTISQGMAVEIEQAEYWKKHIR
                     YFDDEMKEVSNA"
     gene            16147..18390
                     /locus_tag="ACHBFW_000304"
     CDS             16147..18390
                     /locus_tag="ACHBFW_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFM9316652.1"
                     /translation="MLKMTLFTSNQVGNSKNGLFPTKQVVTDKETLVATVKLDHVMAE
                     YKGNYRKGDNFIQSDVIPLDCDNDHSDNPDDWVAPFEVAMQFPDVTFAVSYSRNNMKD
                     KGGKSARPRFHVYFPIPATTDKDEYVELKKQIQSEFPYFDNNAMDAARLLFGTPNSEV
                     EIYEGSKLITDFLEDDLFADWDNAQSEISEGSRNSSMSHIAGKLIKRYGATEDTYHKY
                     LEQSQKCNPPLPEEELKAIWNSAVNFGKKVKQQEGYIPPEQYNSDLSLEPTDYSDLGQ
                     ATVLAREYECKLHYSPSTGYLVYNGSYWEESKPKAQGVIHALTERQLEESETEIEKRT
                     KEMVSNGAFDVLASVGPKKAVTMFNTAQRHSFDLYQHAQEYKKFAVKRRDSKYLSSAL
                     TEAKPMLEIEQRLLDVNEFLLNTPTATFDLRTGKSQDHNSEDYITKQTECAPSDANQQ
                     IWLDALNTIFVGDQELIDYVQMIVGLAAIGKVYVEALIISYGEGRNGKSTFWNVISRV
                     LGNYSGSISADILTSQIRRNVKPELAEAKGKRLLIAAELEEGMRLNTSNIKQLCSTDE
                     IAAEKKYKDPFKYVPTHTLVLYTNHLPKVGAIDKGTWRRLIVIPFLATIDGNKDIKNY
                     ADYLFENAGGAILQWILDGAKKVIAADFHLTVPQVVANAISEYKTANDWLGHFLDECC
                     ETAVDFEEKSGELYAEYRAFCTRTGEYIRSSADFYTALEAGGFKRRRTNKGNKIVGLQ
                     LKSEFLE"
     gene            18528..18737
                     /locus_tag="ACHBFW_000305"
     CDS             18528..18737
                     /locus_tag="ACHBFW_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316653.1"
                     /translation="MKFKQWLQIGIGKRDLRFDEFYEFAMSDVTYPWKKSFDKQVAHL
                     MIHCAEKEYLEILRDAYFEYLTVWL"
     gene            18734..19018
                     /locus_tag="ACHBFW_000306"
     CDS             18734..19018
                     /locus_tag="ACHBFW_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFM9316654.1"
                     /translation="MREKQVEQALVKAVKRVGGICPKFTSPGLAGVPDRLVLMPNGKL
                     GFVEVKAPGKKPRSLQLFRMKQLTDLGFQCFVLDEIDQIPELLERIGGDV"
     gene            19018..20370
                     /locus_tag="ACHBFW_000307"
     CDS             19018..20370
                     /locus_tag="ACHBFW_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFM9316655.1"
                     /translation="MKFVPHNYQKYAIDFIKDHKTAALLLDMGLGKTVTTLTAIKDLM
                     HDDFTIQRVLIIAPLRVTQSTWPTEIQKWDHLKDLSYSVVLGTPSQRMAALQNNANIY
                     LINRENLDWLINKSGFTFDFDMVVIDELSSFKNLKAKRFTSFMKVRHKVDRIVGLTGT
                     PSSNGLMDLFAEFKVLDMGERLGYYISRYRDKYFLPDKQNGMQIYSWKPREDAEQEIY
                     DKISDITISMKSVDFLDMPELVMNEVPVDMGLKEKQKYDEIKADLVLQLKEATIDAAN
                     TAVLSNKLLQMANGAIYDEFNVSHHIHDQKLDALEDLIEGTNGKPILIAYWFQHDLER
                     IKERFNVRQIKTSQDIIDWNEGSIPIAVIHPASAGHGLNLQAGGSTLVWFGLTWSLEL
                     YQQTNARLWRQGQSDTVVIHHIIAKDTIDEDVMLALKRKDKTQSCLIDAVKARLEGSK
                     "
     gene            20367..20795
                     /locus_tag="ACHBFW_000308"
     CDS             20367..20795
                     /locus_tag="ACHBFW_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HFM9316656.1"
                     /translation="MTPKEYLKQAYRLNKRIDDRIKEKAELYQLAVTVSSTTFEEKIS
                     SGTRNTEAPFVRNLEKCYQLEAEIDAEIDKFVDLKRQMRGVIDELTNVDEKIVLINRY
                     LLEESWDKIAEDLHADVRTVFRWHSKALENLKLPDEPIVI"
     gene            20963..21328
                     /locus_tag="ACHBFW_000309"
     CDS             20963..21328
                     /locus_tag="ACHBFW_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM9316657.1"
                     /translation="MPMKPANPCKFPGCPNRTHDTYCDEHKTLARKRYEKYERDPAIN
                     KRYGRAWKKIRARYVAAHPLCEMCLKEGRFTSVEIVHHIKELSDGGTHDENNLMSVCK
                     SCHSKIHMTRRNTKDKTIM"
     gene            21462..22034
                     /locus_tag="ACHBFW_000310"
     CDS             21462..22034
                     /locus_tag="ACHBFW_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM9316658.1"
                     /translation="MAKDGTQRGGRRPRSGERSQPLVDKVAAGKKASRMEFDLPDVEL
                     LIGEDIGEGVELEGNDYPEPSAYLSAKQKDGTVLGADEIYNSTFQWLKDRGCETLIAP
                     RMVESYAQNFARFVQCENALSQYGLLGKHPTTKAVMASPFVAMSQNFQKQANVTWYEI
                     QQIVKENASVEYTGDPNTDAMERLLRSKNG"
     gene            22049..22831
                     /locus_tag="ACHBFW_000311"
     CDS             22049..22831
                     /locus_tag="ACHBFW_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HFM9316659.1"
                     /translation="MIEKVNPQHPDKVADRIAGAIVDLAYAEENNPKIAVEVLIGHGI
                     CHVIIETSTALNLNEISQAINRIAGKIKKDVVIVPQDKELADNQATEIRCGDNGIFRG
                     MPLTDEQLQLSKIARDLYQDHPFDGKYILDGERLIICQSHAKTNELKQKYPNATVNPL
                     GDWTGGPDVDTGATNRKLGSDMADGVTGGGIHGKDLSKADVSVNIYAFLKAQETGMPV
                     ELSCAIGDTEIDGVPYSEIVEVARDYVNELGGFEKLAEWGLF"
     gene            22831..24066
                     /locus_tag="ACHBFW_000312"
     CDS             22831..24066
                     /locus_tag="ACHBFW_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFM9316660.1"
                     /translation="MNTTERFEKVDIDKLVPYARNARTHSKDQVKQIQASMREFGFIN
                     PVIVDKDYNLIAGHGRILAAKEEGIKQVPCVFVEHLTEAQKRAYILADNRLAMNAGWD
                     DEMLAVELSDLQGEEFDLDLLGFETDELDKLLNGNDDAQDDDFDVDEELEKPAFSKTG
                     DLWLLGRHRLVCGDSTKPETYEKLMDGKLANLVVTDPPYGVSYQGKAGTIKNDNLKDE
                     AFYNFLYDAFSCMESVMANDASIYVFHADTEGLNFRKAFVDAGFYLSGTCIWKKQSLV
                     LGRSPYQWQHEPVLYGWKKKGKHQWYTGRKESTIWEFDKPKKNGDHPTMKPIPLLAYP
                     ITNSSLSNCIVLDSFGGSGSTLIACEQTDRICHTIELDEKFCDVIVKRYIEQAGSDAD
                     VSVIRDGKTIAFNDLENPE"
     gene            24140..24517
                     /locus_tag="ACHBFW_000313"
     CDS             24140..24517
                     /locus_tag="ACHBFW_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HFM9316661.1"
                     /translation="MFPTHEQIEGMKKRYPEGSRVELIKMNDPHAPAKGTQGTVTGVD
                     DIGSILIQWDNGSTLNAVYGEDVVHIIKPDFELVFQNGRREGFETFNDAWQYVTDMIL
                     NHDLVWVDLESKTEDTIYIRKGL"
     gene            24519..24713
                     /locus_tag="ACHBFW_000314"
     CDS             24519..24713
                     /locus_tag="ACHBFW_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316662.1"
                     /translation="MKVSKRKIYNIAKNYIVQLPERGDLKAHNSDREDFLDIAVWCLE
                     DALIAAYEAGRKDGQNERKD"
     gene            24697..24873
                     /locus_tag="ACHBFW_000315"
     CDS             24697..24873
                     /locus_tag="ACHBFW_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5049 domain-containing protein"
                     /protein_id="HFM9316663.1"
                     /translation="MNAKIKQQIIEIAESGLTNMFDAIAVQRIAHDRHFYELVIFIEE
                     HKDEYSRFILEGDK"
     gene            24870..25013
                     /locus_tag="ACHBFW_000316"
     CDS             24870..25013
                     /locus_tag="ACHBFW_000316"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316664.1"
                     /translation="MMDYQIYMPDNEDGIEEGYYDWQELVQLLRDNKNNPEAIQFIAD
                     MME"
     gene            25096..26661
                     /locus_tag="ACHBFW_000317"
     CDS             25096..26661
                     /locus_tag="ACHBFW_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM9316665.1"
                     /translation="MDKDSHYDKSRADYAVAFIELLKHTKGQWFGKPFKLMPWQEKII
                     RDLFGTIKANGYRQFNTAYVEIPKKNGKSELAAAVALLLTCGDGEERAEVYGCAADRA
                     QASIVFDVAVDMIRQNPALSKRVKILTSQKRIIYQPTNSFYQVLSAEAYSKHGLNSSA
                     VVFDELHAQPNRKLFDVMTKGAGDARTQPLFFLITTAGNDTNSICYEQHQKALDIIEG
                     RKHDKTFYPLIYGIKQDDDWTDPEVWKKANPSLGITIAEEKVVAACDSAKQNPAEENS
                     FRQLRLNQWVKQAVRWMPMDKWDACSFNADEESLRGRVCYGGLDLSSTTDLTSFVLVF
                     PPEDEDDKFVILPYYWVPEDTLDLRVKRDHVNYDLWAKQGYIQTTEGNVVHYGFIEKF
                     IEELGEKYNIREIAFDRWGAVQMVQNLEGMGFTVVPFGQGFKDMSPATKELMKLTLEQ
                     KLAHGGHPVLRWNMDNIFVRTDPAGNIKADKEKSTEKIDGAIATIMALDRAIRCGNDN
                     GASVYDDRGLLFL"
     gene            26717..27451
                     /locus_tag="ACHBFW_000318"
     CDS             26717..27451
                     /locus_tag="ACHBFW_000318"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316666.1"
                     /translation="MNEYLIQVAPDKISSDPDNTLISRLFYRKWWGKILTEDLTKYAE
                     GIGNYRGETIISFNTIAGATIRLLKCYNQKNFSMPDSHYGRLKVILYSDDITEEMKVK
                     FVKFYDLSHTLANFMPLYVSKPSLNGVKGKGPYHDFPDLFYSDVRDKRYGSSTNADKF
                     IVNNKDYFSKFKDWKSFIELNYLQDFFDDEDYMTFKKLAPSSSFFPYQRKSLTPDEKS
                     KCLEEVNTFLNNAIEIILSRGSRFYI"
     gene            27517..28845
                     /locus_tag="ACHBFW_000319"
     CDS             27517..28845
                     /locus_tag="ACHBFW_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM9316667.1"
                     /translation="MGIIKSLFKSRDKPTNQTIMNPYRFMFGGSSAGKSVTEQTAMQM
                     TAVYSCVRVLSEAVAGLPLHFYQYRDAGGKDKAMKHSLYFLLHDEPNSEMTSFVFRET
                     LMTHLLLWGNAYAQIIRNGKGEVVALYPLMPDKMTVNRDDNKEIYYLYQADVGQVRLS
                     KADVLHIPGLGFDGLVGYSPIAMAKNAIGLAIATEEYGSKFFANGANPGGVLEHPGTL
                     KDPGKLRESWNATFGGSSNAAKVAVLEEGMKYTPISMSPEQAQFLETRKFQLEEIARI
                     FRVPLHMVGDLDHATFSNVEHLSLDFVKFSLSPWLNRWEQALNKSLLSRDEKEKYFIK
                     FNVDGLLRGDYATRQQGYATGIQNGFLSPDDVRELEDLNPIPGGAGAKYYFNGNMLPI
                     EQAGAAYGVNKEESEEQDEEVLEVEESNGSESRQPRGNPRENPLPQRTNR"
     gene            28745..29500
                     /locus_tag="ACHBFW_000320"
     CDS             28745..29500
                     /locus_tag="ACHBFW_000320"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM9316668.1"
                     /translation="MKKFWKWKNQTVLNQDNQEEILERTLYLNGPIAEESWFDDEVTP
                     QIFKDELNSGGGNVTVWINSGGGDCVAAASIYNMLADYKGKVTVKIDGIAASAASVIA
                     MAGDKVLMSPVAMVMIHNPSAVAFGEKVDMEKAIAMLDEVKASIINAYERKTNLSRVQ
                     LAHMMDAETWMDANKAVHLGFADEIIGEIDPDIPVNNSMYSEVKVTNSLTSAIAKKCR
                     IEPPEPKEREEINNTIPADSLMSRLNLMKNWRN"
     gene            29504..30715
                     /locus_tag="ACHBFW_000321"
     CDS             29504..30715
                     /locus_tag="ACHBFW_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM9316669.1"
                     /translation="MNKIQELMEKRAQAWNGAKAFVEAKQDKDGLMSEEDAKTYAEME
                     TKVKNFTMEIERMQSMEAMDRELEKPMSQPLTSQPMTTENKMEPKKTGRASAEYKEGV
                     LEALRSNFRHVSNVLQEGVDTAGGYLVPEEYDSRLIDGMTSENIMRTLATTITTSGEH
                     KINIAGSKPAASWIEEGGELSFGDATFDQILLDAHKLHVAIKVTDELLYDNAFNLENY
                     IIDQFTKALANAEEDAFLNGDGVGKPLGIFAATGGGQVAITTNTQSAVTADDVVNLVY
                     ALKRPYRKNAKFIMNDQTISLLRKLKDNNGAYMWQPALVAGEPDRLLGYPVYTSEFVP
                     TVKAGAPVIAFGDFSYYNIGDRGVRSFDQLRELFAGNGMVGFLAKERVDGKLVLPEAV
                     QILKMKASASS"
     gene            30726..31037
                     /locus_tag="ACHBFW_000322"
     CDS             30726..31037
                     /locus_tag="ACHBFW_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM9316670.1"
                     /translation="MSDLLNKVKANLILEHNEDDELLERLILTALSYAESYQHIESGY
                     YSEHEMPPTTEQAVIVLASNFYESRDGSTGGFFADNVNASKQVWETVNLLLRLDRRWQ
                     V"
     gene            31034..31372
                     /locus_tag="ACHBFW_000323"
     CDS             31034..31372
                     /locus_tag="ACHBFW_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM9316671.1"
                     /translation="MSFGKMNQRIQIVKVTNQKDSAGFSVKQDEVLAAVHAYKEEKNA
                     TEKWVNMATFSSATCLFRIRYIPNLEVTTEMVVISDQKRYQIVSVENVRNRNMYLEML
                     TTEMEVSADG"
     gene            31365..31796
                     /locus_tag="ACHBFW_000324"
     CDS             31365..31796
                     /locus_tag="ACHBFW_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM9316672.1"
                     /translation="MAKALMKMPEDFLLKVSKLENKTDEIIPKVLESGAEVVEGKVRS
                     NLTAVVGSNTKVPSRSTGELESALGITQARQDKSGNWNIKVGFDEPRIDGSSNAKIAN
                     ILEYGKHGQPAKPFLKPAKSQSRKSCIETMKSKMESEVNGI"
     gene            31793..32134
                     /locus_tag="ACHBFW_000325"
     CDS             31793..32134
                     /locus_tag="ACHBFW_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316673.1"
                     /translation="MNILEELNSLFATLHIPVETGEFSDKAPDKYCVLTPLADRFDVY
                     GDNFPTVDVQEVRISLFVKGNYLNTQKQLVQNLLKHEFTITERLFVGFEKDTNYYHVA
                     IDVAKHYEMEV"
     gene            32137..32727
                     /locus_tag="ACHBFW_000326"
     CDS             32137..32727
                     /locus_tag="ACHBFW_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM9316674.1"
                     /translation="MATIGLDKLYYAPITESATTGEETYGTPAMLAKAISAELSVELA
                     EATLYADDGASEVVKEFKNGKLTLGVDDIGRAVAAVLVGATVDQNGVLISTTEDGGTP
                     VAIGFRARKSNGKYKYLWLYRVKFAVPSTSLATKGDDITFSTPTIEGTVMRRNKLDAS
                     GKHPWKAEVDENDTGVEQTTINGWYTSVYEPDFTAD"
     gene            32737..33117
                     /locus_tag="ACHBFW_000327"
     CDS             32737..33117
                     /locus_tag="ACHBFW_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316675.1"
                     /translation="MSEERSAKISLGGDEYELILTTKATKEIASHYGGLDNLGEKLLK
                     SENFELALDEIIWLITLLANQSIKIYNLKNTEKKDELTTEYVELMTSPLQLAEYKSAI
                     TEAMFKGTARNIESETDSKNKAGE"
     gene            33315..35885
                     /locus_tag="ACHBFW_000328"
     CDS             33315..35885
                     /locus_tag="ACHBFW_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM9316676.1"
                     /translation="MIYLADNFGLKIGVEGEKEFKNSLREINQSFKVLGSEMKLVSSE
                     FDKNDKSISSLSARNQVLNKSIDAQKEKITTLENALKNASDSFGENDKRTQNWTVQLN
                     NAKAQLNNMEREVANNEKAIDAMGTEEVDAAKKTDELGNELKDTGDEAEKSGSKFEKF
                     GGVLKGIGVAMGTVALAAGAAAVELGKQVVSAYADYEQLVGGVDTLFKDASSTVQKNA
                     DNAFKTAGMSANEYMETVTGFSAALISSLGGDTKKAAGVADQAVTDMSDNANKMGTDI
                     ESIQNAYQGFAKQNYGMLDNLKLGFGGTKSEMERLLQEAEKISGVKYDISNFADVTEA
                     IHVIQTEMGITGTTAKEASETISGSIDGMKSAISNLMAGLGNSSADIGMLIGNVVEQF
                     QNVIKNILPVIQNIVSALPAVVDAIIQALGQLLPTLLEAVTSLFSQVLETILGLLPTL
                     IPVVVQAVLTIVQTLIENLPLLIDAAFQLVTTLIQGLGEALPELIPITVQAIITIVNG
                     LIENLPLLLDAALQLIMGLAQGLITALPILIESLPQIITGIVNFIISAIPQIIQTGIQ
                     LLTSLVTALPDIINAIVAAIPVIIQNIINAVIGAIPQLIDAGIQLLVALIGALPQIIT
                     TIANAMPQIINGITGTLVNNIDKIINAGVKLLVALVQNLPAIISAIVKAVPQIITGLA
                     NAFGEYVSTMGTIGKNLITGLWNGIADAGSWLRNKISGFFGGVVDNIKDFFGIHSPST
                     LFRDEIGKYMAQGIGVGFDKEMTGVSKTMQKAIPTKLYMPSFDIDTDIHAAVDSTFSM
                     LDLGAKVDALTNITSEMLPTLLKAMDIKVVLNDGTLVGKLAPEIDKSLATLNRRRVAY
                     "
     gene            35956..36357
                     /locus_tag="ACHBFW_000329"
     CDS             35956..36357
                     /locus_tag="ACHBFW_000329"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316677.1"
                     /translation="MLKHKFKAKKPALIGYILLLAIGLFLTAVRWYSGINHGFVFINN
                     QINSHISNFALSFLFYLVIGYLWLLMGTNLRVLTAMGAGLIVANIVCETMMTFLNTPD
                     LIDAIYGISGVLVVYAYLAITKKIGLEETTE"
     gene            36434..37126
                     /locus_tag="ACHBFW_000330"
     CDS             36434..37126
                     /locus_tag="ACHBFW_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM9316678.1"
                     /translation="MNAFILDGTINSRTDLGLRITEVPVIPVTERVIDSIEIDGREGD
                     LTILKGWKDVTFGFKVVIWKSDVWTSWQIILPQILNAKTIAFSNEPNVFYPIKYAKAS
                     GLTQILSSMWEFEMELTCSPFRYKTNVATINRTTSGTVNNPGNIYSLPKIKVYGNGTR
                     TLTINGKPIVLNLTNGNLTLDSDLKECFYGDVAANQYMTGDFPEFRVGSNTVTLGTGI
                     TKVEIEPRWRYL"
     gene            37123..>39084
                     /locus_tag="ACHBFW_000331"
     CDS             37123..>39084
                     /locus_tag="ACHBFW_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM9316679.1"
                     /translation="MITLYNKTESNFNHNGLAVLDGNILNPVVSEELNGLFSFEFDYP
                     IHAPHSEELLPEMIVKAPVPELSDQLFRIVERDDALGGFLHIVAHHIFYDLATNFIED
                     TYIVNKNGSGALTQLLGATQFMHRFTGTSNISAVNNVRLVRLNPVEVLLDSDLENGFQ
                     SSYGGEIVRDNFTISMLTTRGSNNGVQIRDKKNLTGYKADVDYSGIVTRIMPQGYDGL
                     FLPEKYVDSPNINQYVQPKIKVIKYDNVKVGDEEDEFTQAEAYDELRRLAGLEYSANK
                     VDLPKATYDVEFAPLERTEEYKDFAQLETINLGDTVSVIHAEDGLNITARMVSYKYDP
                     FLKAYISVTLGNITPKFTDVAKDINRVDNKAEQAKDDANYALSSANGKNTNFYGPTTP
                     NNPKLGDVWYKENGDKFEMWVYEIRDGVTQWYPLMTDMTAEEVKQAVEQAQKDANTAT
                     EKANQAFNDAVNALNAAESVGASVETLTTSVEQNTSKISLVASGMSTLEGRVITAEST
                     LTVQAGQIASKASQSTVDNLTGRITTAESTITQNADRFELSLSKTNKAVNVLAGMSIA
                     DLQWAVGGLNTNDGKENTYGGYVRSGFTRVRNGEKYLLQTFVGGKLYSTYATAYLLYY
                     KEDKSFLSYTQFGNTTTPLTVPSSAAFIRI"
BASE COUNT        12169 a         7150 c         8983 g        10782 t
ORIGIN      
        1 aggtgtagta tgtcaatttc ttataataaa ttgtggaagt tattgattga tttgaatatg
       61 aacaaaactc aacttcgtga aatatctggc gtcagtccca atgtaatagc aaaactcggt
      121 aaaaatgagt tggttgcaat ggagtcatta ttgaaaattg ctgctgcctt aaatgttgat
      181 gttagtgaaa taattagttc agatgttata gatgcggagg atgttaaata aatggcaaaa
      241 aataaagttt taaacatcac tgaaattgaa gatggtgtaa aaaaaatact ttcaaatagg
      301 ggcgatgatt tcatcactca atttttgtcc ttgtacgata tccccaaaac gtcgattaca
      361 cgtgcaaaat caaaattcga taatggtgaa ccatttgtga ttaagaacaa acttcattat
      421 gaagaaattg aaggcaacgt tgtcattgcg attgatgcga ttgctcaatc aatcaaagag
      481 caaaaatcta aaccacgata tatcttggta aacgattatt tagatattgc tgcaattgat
      541 acgaaaactc atgaaacttt gaatattcca attgaagagc taccggctaa tgctgacttt
      601 ttcttagcgt ggaatggcat tgagaaggct gattatcaag ctgaaaatcc agctgaccga
      661 aaagctgctg aacgttttgc aaaattgtat gatgtggtgg ctaaagataa tcctgatgcc
      721 aacgagcatg catttaactt gttcttgatt cgagtgctgt tcttgttgtt tgcagaagat
      781 acaggaatta tgaataaggg ttcctttaca aatgttctaa aaacaagaac ggctgaagat
      841 ggttcgaatt ttaacgaagt aatcaaagaa ctctttgcaa tcttggatat taatgagtta
      901 gctcgcaatg gcaaggatga gtggctactt gaatttccat atgttaatgg aaaactgttt
      961 ggtgagccac atgtggattt gaccttttca aaagtttcaa gagagctgct tattgaagca
     1021 ggggaattat tgaattggaa tgaaatcaat cctgatatcc ttggcgcaat gattcaatca
     1081 gttgcttcag cagaagaccg tcacgttgcc ggtatgcatt atacaagcgt gccgaatatc
     1141 atgaaagtca tcaagccgtt attcttagat gatttgacag agtcatttga aactttaaag
     1201 cttcgcagtg aagaaaacga gctgaaggac attactgaaa aaacaagaaa cgataataag
     1261 agaacaatca tcaatgatct aagtgctttg ttgacgcgaa tttctaatat taagtttttg
     1321 gatcccgcca gtggttctgg taattttctg atcattgctt ataaggaaat ccgtagactt
     1381 gaaattaaga ttttgcaatt gttgggcgac ttacaaaata atgattccat gccactatca
     1441 gtgattcatc ttggtaattt taatgggatt gaattggatg attttgcgca tgaagtagca
     1501 agactttcat tatggattgc tgaacatcaa atgaataaag agcttgaaga agcaatacca
     1561 ggtaccattg ctgagttgtt accgttaaaa gatgctggga atatagtgtg tgccaattct
     1621 cttcgaattg attggaatga ggtgttgcga ctcaaagaaa atcatgaaat atatataatg
     1681 ggaaatccac cgtatctggg caaaagtctt caaaatgctg aacaacgtga agatatggaa
     1741 aaacttgttg gcgatattcg aaaatataaa ttgcttgatt atatttttgg gtggttttgg
     1801 aaagctaaac aatatattgc aacatctcat ggctcgttcg catttgttac tactaattca
     1861 atttgtcaag gtgaacaagt tagtatcctt tggccgtacc ttttaaaaga tgaggtaaga
     1921 atttcatttg catatcagag ctttaagtgg aacaacaatg caaagaataa tgccggtgtg
     1981 actgttgtca ttattggtat gacaaataat gtgaatattc atcctaagtt atatttagag
     2041 aatgggtcag taatgagtgt ttcaaatatt aatgcatatt tagctaatgg tgaaaatgta
     2101 tttgtcaacg aatcaagaaa tagtatttca aatttaccct ctttgaaatt tggcagtatg
     2161 gcaaatgatg gtggtaatct agttttttca gtggcagaat actggaattt gattcaaaaa
     2221 tatccagata ttaaacattt atttaagaaa tatttaggat caacaggatt aataaatgga
     2281 gacttaagat atactttatg gttgaccgag gaagatgcaa ctagatacaa acaaaattct
     2341 cttattaaag aaacaataaa aaatgtaaaa agttataggg aaaaaagcaa gagaaaagaa
     2401 actcgagcac tagctgagaa gccatggtca tttggtgaag ttcgttatga tgaaaccagt
     2461 gagaatagta ttctaattcc tcgtgtgtca tcagaaaaaa gagattatgt tcctatgggt
     2521 atagttggga gcgatactat aatttcagat tcggctatgg caatctataa cgctccaatg
     2581 tggctgcttg gtctgttaga atcccgaatg catatggtgt ggctgcgctc tgtcggtggg
     2641 aaattagaaa ctcggtaccg ttattcagcc gggttagtct ataatacttt tccgattcag
     2701 gatttatccg cccagcgtaa aaatgaaatg actcgcgtga tgacagaaat tcttgattta
     2761 cgagaatacg aaggcggcac gttagccgat ttgtataata aagacagtat gccagaatca
     2821 ttacgtaaga aacaccagga actggatggg attgttgata gagcttatca gcaacgcccg
     2881 tttgaatctg acgaagatcg acttggaaca ttactaaaac tctatcagga gatgacaatc
     2941 aatgactagt aatttatttg aaattaattt tgataaaaat caatcaaaaa caacaaatga
     3001 cctcggtatg cgtgaaatgc aagaacgtgt ttatgaaaag cgtgcgtcac agtatttgtt
     3061 ggtaaaatca ccaccagctt caggaaaatc acgtgcattg atgtttgttg gcttggataa
     3121 actccataat caaggaatta aaaaagtggt tattgcagtt cctgaacgct caattggtaa
     3181 atcatttaaa gatactgaat tatcgaagtt tggtttttat tgggattggg aagtaaagcc
     3241 aagtgacaac ttaactattg ggggcggtga aaaatcaaag gttcaacact ttgttaaatt
     3301 catgaactcg actgacccaa gtgataatat cctgattgcc acacatgcta cgttgcgatt
     3361 tgcttttgaa gagttggacg attcattatt tgacaatacg ctattagcga ttgatgagtt
     3421 ccatcatgtg agccgtgatg ataaatctgt gttaggtaat gccttacgta gcatcatgtc
     3481 aaactctaat gcgcatatct tagcaatgac tggttcatat ttccgtggcg actctgttca
     3541 gattctggaa ccagccgacg aagataaatt tgataaggtc acttacactt attatgagca
     3601 actggaagga tataaatatc tgaaatcctt tgcaatgggg tatagtttct atcgtggaaa
     3661 atatacagat gctttggata aagtaattga tgtcaacaaa aaatcaatta tccatattcc
     3721 taatgttaat tctggtgagt ctactaaaga taaatacgcg gaagttgatg aaatacttga
     3781 tttattgacg gacggtggta atatcacaca gaacgaggat ggtatttatg aaatcgaacg
     3841 tccagatggt aaaaaactat tagtggccga ccttgtgaat gaagaaggac gtgaaaaagt
     3901 ctcagcatat ctagcaagta ttacggatga cattaaagat cttgataagc ttgatatcat
     3961 cattgcactt ggaatggcaa aagaaggctt cgattggcca tttgcggaat atgcactgac
     4021 aatcggttat cgtaactcat taactgaagt gattcaaatc attggtcgcg taactcgtga
     4081 cagccccaat aagactcatg cacagtttac aaatcttatt gcgcagccag atgcacagga
     4141 tgacgaagta ttattcgcgg ttaacagtat tatgaaggca atttctgcat ctctacttat
     4201 ggaacaagtg ttggcgccag tttacaagtt taaaccaaaa gatggtaagc cagacaaacc
     4261 aggtgatctt tcaatcagag gactgaaacc tgctgatacg acggaacgta caaagaaaat
     4321 catcgaaaat gatatggaag atttgaaatc tagcatttta cagagccaag atattcaaaa
     4381 tgcgattgtt tcaggtgctg atgcgaagat gattaataag acgttgattc cacgtgtcat
     4441 cattgatcgt tatcctgatt tgacgaacga agagttagaa accgttcgtc aacatacagt
     4501 tgccgatata aatattgcgg ctggacggac aacaacttca gaagatggta gccgcgaaat
     4561 tattaagatg gccgatcgtt tcattaacat tgatgaattg aatattgatt taatcgatca
     4621 agtgaatccg ttccaacgtg cgtatgaagt gattagtcgt gatattgatg caccgactct
     4681 tcggtttatt caggattatt tgtcatcaaa taagtatgat tttactgacg aacaattggt
     4741 tgcggcctac caagctgcta agaagttccg tgcagaaaat ggtcgaaatc ctgatcgaaa
     4801 tagtaaagat gatggtgaaa aatacttggc ctttgctttg ctacgacttg ctgagataaa
     4861 acgtgaaaga gaggcagcga aagaagatgg cgaatagttt agatagcatc tttgaagatg
     4921 acgcttttga tgatttaacc aagcaagttg aaaaaaagaa agttgtaaaa caagacccag
     4981 aagttgctaa atttcaggag attatagact ttgtgaatga aaacggacga gagccagaaa
     5041 aaactcctga atggtctagt gaacgcgcat tgtgggctcg tcttagcgga tttcgaggaa
     5101 aacccgaaag gtctgagaag gttaagaagt atgacactct tggtttattg gagaaagaat
     5161 tcgaaccgga tggagagttt ctcgctgatt cagttgaaat cgatgacgag ttgaatttag
     5221 atgatatctt aggctcagat gatatgattg atgatttttc agacttgttg gatgtttccc
     5281 gctatcgtag aactgtgaac gctgcagata aaattggccg cagaaaacat gcaagtcatt
     5341 ttgaaaagta taagagcttg ttcgatcaag tgcatgcaga tatcgcatct ggcagacgaa
     5401 aaattatccc gtttgagcag tatgatattc atgaaaatag attctacatc caaaacggcg
     5461 taatgatgta tatcgtttcc atttcgaagg atacctttat cgctgataat gggaaaacaa
     5521 atgccagaat gcatgttgtt tatgagaatg ggacagagaa taagaatctg ttgttacaat
     5581 ctttagcttc gtctctatac tcgactgaac gtcatggaag gatggtgaca gagccggtag
     5641 atgaggaaac actggctgaa agctttggag aagagtttac gacaggttat atctacgtgc
     5701 tgaagtcatt aagcacgaat ccggatatta gcaagtggaa taacctgtat aagattggtt
     5761 tcactcgaaa tgatgttgaa agtagaattg ccaatgcgga aaacgacgtg acctacttaa
     5821 atgcaccagt gaaggtcatg ctttctgctg aagtacaaaa tgtcaacgct cagctacttg
     5881 agcgcactct ccatcatgta ttccaggaca aacaagttgt ctttcaggat ggaaacttca
     5941 aaaaagctac tgaatggtat atggttccat tgaatgatat tgaatcgaaa atcaatgaag
     6001 taatcgctgg attacaaaaa taaatatgaa aatgcactgt actcatgaat cgaatacagt
     6061 gcatttttga tatttaatcg cgaacgattt caacaatatc cgataaatca caatcaagtg
     6121 cttcacagat gcgaagtaag atatctgttg ttacattttc gcctttattt agttttgaga
     6181 gtgttgctga actgatatca gtcatttttc tgagttgtgc tttattcagt cctctgtcga
     6241 ttaggagttt ccaaagcgga ttgtagctaa acttcatgta atcattcctg ttctagttat
     6301 aaatatcgta tagatatcca tagcgtccat tgtttttatc ttcagtaaag acaatctgat
     6361 ttacttgttt atcatcatag tttagtgctg gcaagttcgt tgtattctca acgatgataa
     6421 gttgtccttc gttttgatga tccataaagt atttgaaaag tccttctttc atattgtcat
     6481 cgccatcttg ttttttgcgt tcatctaatc caagaagagg ggaatcgata atgaacacag
     6541 aaggtgtgta cttagcgtga ttatacaaga gatgtcgata tgccattacc actactgaat
     6601 tgataaacgc tgtatatcca tgtccgtttt cggtccattt tagatttcca ttgattttga
     6661 tgtcgaaaat atccttggcg aaaaatgcgt tacgaaattc attaggttta taatgcatat
     6721 tacgtaacat ggagtccagt aactcagaca tttctttgta gaattctgca tcaaatttct
     6781 ctttaggttt ataaaggata cgttcgtctt ctggctcatt ctcgattttt tcaagatcac
     6841 ttttaagctt attttgcatt ttttccatcg agattagctg ttgtttaata tccctgtaag
     6901 tttcgatttt attgattcga tcctttaacg tctcagcttt cggttttagt tcatcgttga
     6961 ttgctgcatc aagcgatttc tttcttgttt taagctcatc aagctgttcc attaactctt
     7021 gcagttcaac ttcggtatca tgagattctt ccgtcaaatc tgataattga gagagcaatg
     7081 aatgtaattc tgacctagca gtatcgatgt aggttgtttg gacaggttta cttagttcgc
     7141 cgttacagaa tggacagcta tgtgaagtat catgttcatt atgatcattt aataatcgct
     7201 caccatctac gattagtgtt agtcgttcaa tatccgattg atattgataa tgtaactgtt
     7261 tgtacctatt caaaagaagt tgagtttcat gaagatactg ttccttttga taaatctctt
     7321 ttatcaagat tttgctgctg gctaacgcat cattgatttg cttttcaatt gtttcaagtt
     7381 cagcgaacat tccatcgaca atttgttgaa aatcatcaga gggagcctca ggtaattctg
     7441 atttagccac ttcgtagttt tcatataatt cagaaaggct tgaattgatg tagttaatca
     7501 ccgagttcct tctaattttt ttatgttctt ttgtttcgtt ttcttccgag ttcgcaaaat
     7561 ctgaaccgta tagcagaagt aacaacgatg ataaaaaagc tgtgcgttgg gttgtttccc
     7621 caggaaaaag aacagaggcg ctggtattga ctctatcttg tggaataagt gtgctaggcg
     7681 ctaatgtccg ccaagttaat ttttgacgtt tgaaatcctc attcttgata atctgataat
     7741 catcatcgat gcctaaaagt tgtaaccaaa aatggctaat tggatttttc ccagatcgtg
     7801 cattatattt ttcataatct acatattcac ctgaactcgt tattttggat accaaaatgg
     7861 agttgctatc aaattctcta gttaatgaat atttgccatt agtcgattga ataatcagtt
     7921 caactttact gtatccggtt gcatcggttg aaaagggagc agcgtctttt ttcgcgccat
     7981 agagaaaatc gattgccgta acaatgctag tttttccggt atttgaaggg ccagaaatta
     8041 tgtttagtcc attggtgaac ttgacagttg catcttcacg atctttgcct tttatggtaa
     8101 cacgttcaat ataataattt tgcattatca ttgctctcct ttaaattcct ttgcgcgttg
     8161 gtttatttgt cgcagaagct caacgtcact tttatcttga atgtatttaa cagattcgtc
     8221 agctatttgt aagtattttt ctgcatatac cgatacgaat tgtgatatca actttaggcc
     8281 atcttctgat aaagcatact taaaaccttc tttgctttgc gatggttgga taacatgttg
     8341 cagtacaaga tttttgactg catctttaac taattcacgt cgcgcaataa attcactgaa
     8401 tctatagctg ttatcaccat ttaagttgta atcggaaatt tcaaagtcgc gaccatacgt
     8461 ggtgatgaag tctgtgatta aaattctatc agcgctgaca ggctctttaa attcagagag
     8521 cagtaacatt actcgaaccg aaatttcaaa tggagtatta aacaaactat tcatcttcag
     8581 tcacccattt gatctttccg tcattaacca gcatgtggca aatgcctttt ttagcagaat
     8641 tttgcagcaa tcccggtata aacgataaga ttgatccgtt taatgttata acagacgact
     8701 gctgtagaac ggctttgagt ctctcaacgc catcatcgta gtcttcttca tagacttcct
     8761 cgatgccgtc gtacatatct tcttttaaag cttcaaagtg ctcagttcct tcctcttgtg
     8821 taaagttatc acggacccca cgacgtatgc tttcagcttt atagtaattt cttcgctgtc
     8881 tcttgaaatc ctttagccgc gaatttggca ggtcgtttgt atgctcgtat acttcgtatc
     8941 catcggcatc tgcatatgct gaaaaaagag ctatgacata cgtcatctcc tcagctctaa
     9001 tatcaagggg aactggcaaa tcgtcaaaga attttttcac ttcatcatta taagagaaat
     9061 caccattagt gttgattctt aaacttgcga gctctaattc gctaagcttc ggcttggcct
     9121 tttttgtttt tacttttgtt tgagaagcaa ttgaggtgat ttcatcaagt aaaagatggg
     9181 ccactatttc agaaacgtct gtatctttgg ggattaccac acctttatca atcaattgtt
     9241 gcttaaggac ggttggagca tcatctataa gacgttcata aataaaagtc gtgcatttgt
     9301 cgatgttgaa ttttccagca acccgaactg catactcttg tgttaatggt cgagtaccat
     9361 tgtaaaatct tcgtaggtta tcatcagaaa aaccgaaaat ggtggcctcg tcatcatttg
     9421 aaacactatc cagtaaagca attaaaaaat cctgtggctt cacgtactcg cttcccataa
     9481 acggataaag cgtttgagta atttcactta tttccaaaat acaacctcct ttgttacgtc
     9541 cgccgttgac cgccgtttgc cgccgatgac tgacgaaaac aagggaggcc tttttctata
     9601 attaaaacat agtagtttaa ctatttaatt ttatcattta tggttcataa acactacatt
     9661 atgtttcaag aaagtgaacg aatttatctt aaaagcgaat agtcctacta ataaaatata
     9721 acaatgcctg agttgcaata gggcaaggtt acaaatactg aaaagaactg gttatccaga
     9781 aaatttttca gatattgtat gccttacttt attgcgctct tttttcgtga tgagggacgg
     9841 tgtacgtatt ggcacggtcc ctgtttgttt tccttgctca aaagctcttt caggcaagaa
     9901 agagggatct aatgagtaga tttatgaaaa cacaccaaaa cgaccgcagc acttacaagt
     9961 acgtgtttga gcgtactgat gccaatggac gtatccaaaa agaagtggtt gagattcgcc
    10021 caggcgaaga cggcgtcact gagcttaaca ttcaggagct gcatcgcgct gacgaccgtg
    10081 cgatttacaa cttttataaa aacgcacgtc ctgaacgcac agaagaagaa aaagaaaaga
    10141 tcaaggcttg gaaagagaag ttccgtgaag actttaataa tgagcacggc tattatccaa
    10201 tggacgaata cgtggatggt gaagccaacg attacttcaa acgtaactac aatttgtcgc
    10261 tcaatgaatt tgatgatgaa tgcgatgagg gggacaaccc catccaatca ttggtttacg
    10321 atgcttacca agataatgaa gaagatcctc gtatcgatcg tttgcgtgaa gtcgtgacgc
    10381 aattattacc gtaccaccaa gaaatctatc acatgctcgt cactgaaaac atgtctcaga
    10441 ctgaagtggc ggacaagctt gacaagaaaa aacagtcaat caatcgcacc tttggaatca
    10501 tcaaagacaa gatcaaagaa ttattttaga aaaaagttac gggggagttt actttccccg
    10561 ttttccttcg cctgtattgt gtaagggagt agatcctaaa caaataaaga tgaaggagga
    10621 ccatcatgaa cctaaaacac aaaatccgag taaacgtgac tgatgacacg ggtaacaaag
    10681 aaactgtcct acaaggtggt gtgcgtcatt tgcctcgctg gtttgcacgg tggctgttcg
    10741 gtaagggcaa catggacaaa attctcgtgc tcacaccagg ggacacaatc gaatcagtcg
    10801 aaatccgtga agttaacaag aagggagcgt aatgacgtga gtaaaacaaa actatttctt
    10861 caggtcgttg aggacgtcca agcgttagct gatagcttac aagagttggc tgtggcgatg
    10921 aaaaccaatg aacctgaacc acaagttgaa acaccagaac cgacaatgga aacaaaatca
    10981 gaagtcaaac aaccgacgct tgaagaagtc cgtggtttgt tggcacgtaa atctcaagaa
    11041 ggtaagtcaa ctgaagtgaa agcactgatt gagaaatatg gtgccagtcg cttgagtgat
    11101 gtgccgacag aaaactacgc ggctttggtt gcagatgcgg agggattgta gatgccaaca
    11161 caacacgcag tcctatccgc atcttcaagt aatcggtgga ttcactgtcc gccaagtgtt
    11221 cgcttgtcag agcactttga aaacaagtcg agcccgtatg cccaacaagg cactgatgct
    11281 cataccttat gtgagtacaa actgaataag ttcattggta atgacgtcac agacccaaca
    11341 tctacgcttg aattctacga cgaggagatg gagcagtgtg ctgaaagtta tgcgaccttc
    11401 gtcatggaag aagtcgctaa ggcgaaacag acaacagttg actctattgt aatagtagaa
    11461 cagcggctcg acttctcaag gtacgtccca gacggctttg ggaccggcga ctgtctcgtc
    11521 attgcggatg gaaccctatc tgttattgac atgaaatacg gtctaggaat tttggtagat
    11581 gcgtatcaga acccacagat gatgtgttat gccttgggag cgttggagct tttcgatgtt
    11641 atttatgaca ttcaagaagt caagatgacc attttccagc caaggcgtga gaatgtgagt
    11701 acgtacacac tgctgaaaga agaattactt cagtgggcaa aagatgtgtt gtcgcctgcc
    11761 gcagatttag ctttcaaagg tgagggtgag taccaatgcg ggaaatggtg tggattctgt
    11821 ccggcgaaga atagctgtcg tgcacgagca gaccataact tgaaactggc gcaatatgag
    11881 ttcaaaccac cagagcttct tagtgatgat gaaatcgaag aaatcatcgg taaagtggat
    11941 gacctggttt cttggagtaa cgatatcaag gactgtgctt tgaaacttgc actcggtggc
    12001 aaacaatgga ctcatcacaa attagtcgag ggtcgctcta ctcgtaagta ttctaatgac
    12061 aatgatgtcg ctgcggcggt catcaaggct ggatacgacc catatgataa aaagttactt
    12121 ggtgtcacag caatgacgaa ggcactcggt aaagagaaat tcaatgaaat cttgggcgaa
    12181 tacatcatca aaccaaaagg caagctgacg ctagtcgatg gttcagataa acgacaagcg
    12241 gtcacagtaa acaacttaaa cgaagaattt aaaccattaa cggaggaaca ataatatgtc
    12301 taaaacaagc aaagtaatca ctggtacaaa cacacgtctc tcatacttcc acggttggga
    12361 accagcatcc atcaacgggg gcgcagagaa atattcaaca gcagtcttga ttccaaaatc
    12421 agataagaga acgattgata ccattgaaaa agcaatcgat gcagcaatcg aagagggact
    12481 tgcgaaattc ggtgggaaga aaccaaacaa agcagaaatc aagctgccat tacgtgatgg
    12541 ggatatcgaa cgcgacgatg aagcatacca aggccattac ttcattaatg cgaactcaac
    12601 cactgcacca caaatcgtgg atcagcacgt tcaaccaatc ttggatcgtt cacaagtgta
    12661 tagtggttgc tacgctcgtg tgtcgattaa tttctatgcc tttaacgcaa atggaaatcg
    12721 cggggtagcg gcagggcttg ggaatatcca gtttgtacgt gacggcgaac cactcggctc
    12781 acaagtatct gcaagcgatg agtttgaaac cttggcggat gatgatttct tggcttaatc
    12841 attaaaacaa aaacggccat ggcagaagtg ctgtcgcctg tttgattgga ggaagaaaat
    12901 gaaacatttg tcattggatt tggaaacgta tagtagtgtc gatttatcga aatgtggtgt
    12961 ctacaaatat tcagagtccc ccgattttga aatactgcta tttgcataca gtgtagatgg
    13021 tggtcccgtc gaagtggttg atctgaagat ggacgaaact atcccggctg aaatattaac
    13081 ttcgctatca gatgaaacag tgattaaaca cgccttcaat gcgagttttg aacgtgtgtg
    13141 tttgtcacgc tttctggggt tggatggctt tttgaatcca cgagggtggc aatgcacaat
    13201 ggtgtggggt gcgacattag gtttgccgtt gtcacttgta ggagttggtg ctgttcttgg
    13261 tttggataaa caaaagctca cagaaggaaa agacctcatc cgatactttt gcgtgccatg
    13321 caatccgacc aaagttaatg gtggacgcac gaggaactta ccacaccatg cgccaactaa
    13381 atggtcagca tttaagacat acaatagtcg tgatgtggaa gttgaaatag ctatccaagg
    13441 aaaactttcc aattttccag tgcttgattc tgtttggaaa gaatactggc tggatcagga
    13501 aatcaacgac cgaggaattg agattgataa ggactttgtg gaagctgccg ttcagttgga
    13561 tgaattatca aaagctggat tgaatcaaca attgcaagaa ttaactggac tagaaaatcc
    13621 aaacagcgtg cttcaaatgc ggggatggtt actagaccac ggacttgaaa ccgagtcact
    13681 cggtaaaaag gcagtggccg aactactcga acaacctatt gatgaaaatt taaagcaagt
    13741 cctggtctta cgtcagcaac tcgccaagtc atccgtgaaa aagtatcaag ccatgcaaaa
    13801 ctttgcgtgt tctgataatc gttctcgtgg tttattccaa ttctatggag ccaaccgtac
    13861 cggacgcttt gcaggacgtg gcatccagct tcaaaacttg cctcagaacc atatgtcaga
    13921 tttatctgaa gcgcgtagtt tagtaaaaca aagcaatttt gaaatgctta acattctcta
    13981 cggcaatgtc ccgaacgtac tctcagaact cctccggaca gcgtttgtac cacgtgaagg
    14041 gatgaaattt atcgtatctg acttctcagc gattgaagca cgtgtgattg cttggctgtc
    14101 gggtgagaag tggcgtcaag aagtattcaa aaatggtggc gatatttact gtgcgtctgc
    14161 cagtcagatg ttcggtgtgc cagttgaaaa gcatggcgtg aatgggcatc tacgtcaaaa
    14221 agggaaaatt gctgagcttg ctctagggta cggaggatcg gtgggtgcat tgaaagcaat
    14281 ggatgctctt gaaatgggac taactgagga tgaattacag ccgcttgtta attcttggcg
    14341 agactcaaac attaacataa cggagctttg gtgggctgtt gataaggcag taaagacttg
    14401 tgtgaaagaa cgacggagca cagcgacgca tggcatcaag tttgaataca agtcgggttt
    14461 tctgttcatc caactcttgt caggacgtaa attagtttat gtaaaaccaa agattggtga
    14521 gaacaaattc ggcggtgaag ctgttaccta tgaaggtgtc ggtgctacca agaaatggga
    14581 acgtcttgaa agctatggtc ccaagtttgt cgaaaacatc gtgcaggcaa tagctagaga
    14641 cattctctgt ttcgcaatgg agaatcttcg taagtatcgc attgtcgggc acgtccacga
    14701 cgaagtgatt atcgaagtgc caatggaatc gaaggttgaa gacatctgtc agttgatgag
    14761 tcaaacgcct aagtgggctg aaggcttgct actgaacgct gatggatacg agtgtgaatt
    14821 ctatcaaaag gattagtcaa tgtggagtca gaaatggctc catatttttt tcaaattttt
    14881 ttatcaaaaa taaggttcga attgggttct ttcttcgctt atagacgaag gcaaaaaaat
    14941 caaaaataaa ttcgagtcag agtttacttt cgaccatttt cttcgcctgt aagttagaag
    15001 gggcaaaatg cctctcctga aaattctatt acaggaggtt cacaagatga acgaattgaa
    15061 agtttttgaa aatgcggagt ttggctctgt acgcacggtg atgattggcg atgtccctca
    15121 ctttgttggt aaagatgtag catcaattct tggctacaca aatccacaaa aagcattacg
    15181 agatcatgtt gatgaggaag ataagacggt gaacgaatcg ttcagcgtta atgggacaat
    15241 ggctatcctt atcaacgaaa gtggattgta ctcactcatc atttctagca agatgccgaa
    15301 cgcgaagaaa ttcaagcgtt gggtgacaaa tgaggtcttg ccgtctatcc gccaacacgg
    15361 tgtgtacgcg acagaagatt tcatcacaaa gtcaatcgaa gatccagcat gggccatttc
    15421 agtgttgaag cagctacaag agaaaaaaga aatggtggcg atgcaacaac aaatgatttt
    15481 ggagatgaaa ccaaaagtta gctactacga tttgattctt caaaacaatt ctgtgatgtc
    15541 gatttctaaa atctcgaagg actacggtat gtcaccacag gcaatgaaca agttgcttca
    15601 tgaactggga attcagtaca agcaaggcaa gatgtggttg ctttatcaga agtatgccaa
    15661 tcaaggctac acacaatcaa aaactcatgc gatagatgca gagcgcagcg tgatgcatac
    15721 ctactggacg cagaaaggtc gtttgttctt gtacgacttg ctgaaatcga agaagaacat
    15781 cttaccagtg attgagcgag gtgataataa tgcctagacg taaagattac agaacatcag
    15841 tctatgtctg ctcgccgttc tcaggaaatg ttgtcgtcaa ccataaaaac actaagcgtt
    15901 actgccgtat tgctcttgac cgtggctaca tgccagttgc ggcacatctg atgtatccac
    15961 aattcatgga cgacgcaacg gagcgtgatg tggcaatgca catgtcactt gttctcatgg
    16021 gcaaatgtga ggaagtctgg gtggtgggtg acaccatctc ccaaggcatg gcagttgaaa
    16081 ttgaacaagc ggagtattgg aagaaacata tcaggtactt cgatgatgag atgaaggagg
    16141 tctcaaatgc ttaagatgac gttattcaca tccaatcagg tgggtaattc caagaatgga
    16201 ttgttcccaa ccaagcaagt ggtgacagat aaagaaacac ttgttgccac ggtcaagctg
    16261 gatcatgtga tggctgagta caaaggcaac tatcgcaaag gtgacaactt catccagtct
    16321 gatgtgattc cacttgattg cgacaatgac cactcagaca atccagatga ttgggtagcc
    16381 cctttcgaag tggcgatgca gtttcctgat gtgacgtttg cggtcagcta ttcacgtaac
    16441 aacatgaaag acaaaggtgg caaatcagca cgtccacgtt ttcacgtata tttcccgatt
    16501 cctgccacaa ctgataaaga tgaatatgtg gaactcaaga agcagattca aagtgagttc
    16561 ccatactttg acaacaatgc gatggatgcg gcacgtttgc ttttcggaac accaaactct
    16621 gaagttgaaa tctatgaagg tagtaagttg atcactgatt ttctcgaaga tgatctgttt
    16681 gctgattggg ataatgcgca gtcagagatt agcgaaggca gccgtaattc aagcatgagt
    16741 cacatcgcag gaaagctcat caaacgctac ggtgcaacgg aagatacgta tcacaagtat
    16801 ctcgaacaat cccaaaaatg taatccgcca ctgccggagg aggagcttaa agctatctgg
    16861 aatagcgcgg tcaactttgg gaagaaagtg aaacaacaag aaggctacat tcctccagag
    16921 cagtacaact ctgatttatc acttgagcca accgactact cggacttagg tcaagcaacg
    16981 gtcttagctc gcgagtatga atgtaagctt cattactcac cgtcaactgg ttaccttgtc
    17041 tacaacggca gctattggga agagtcgaaa ccaaaagcgc aaggtgtgat ccacgcattg
    17101 acagaacgtc aactggaaga atctgaaacc gaaattgaga agcgtaccaa ggaaatggtt
    17161 agtaatggcg cgttcgatgt acttgctagt gttggtccga aaaaggcagt caccatgttc
    17221 aacacagcgc aacgtcatag ttttgatttg taccaacacg cacaagagta taagaagttt
    17281 gctgtcaaac gccgcgacag caagtatctc tcgtcagcat taaccgaggc aaagcccatg
    17341 cttgaaatcg agcaacggtt actagatgtg aacgagttct tgctgaacac accaacagca
    17401 acctttgatt tgcgaacagg caaatcacaa gaccacaact cagaagatta catcacgaag
    17461 caaacggaat gtgccccgag tgacgccaat cagcagattt ggttggacgc tctgaacacc
    17521 attttcgtag gtgaccaaga gttgattgat tatgtccaga tgattgtagg acttgcagct
    17581 attggtaaag tttacgttga agcgcttatc atctcgtatg gtgaagggcg taacgggaag
    17641 tccacgttct ggaatgtcat cagccgtgtg ctcggtaatt actctggcag catctcagct
    17701 gatattctta caagtcaaat ccgacgcaat gtgaaacctg aacttgcgga agcgaagggg
    17761 aaacgcctac tcatcgcagc cgagcttgaa gaggggatgc gactcaacac atctaacatt
    17821 aagcagctct gctcgacgga tgaaattgcg gctgagaaga aatacaaaga cccattcaag
    17881 tatgtcccaa cgcatacttt ggtgctctat accaaccacc tcccaaaagt tggagcgatt
    17941 gataaaggaa cctggcgtcg tttaattgtg attccattcc ttgctacgat tgacggcaat
    18001 aaggacatca agaattatgc ggactacctt tttgaaaatg ccggcggagc cattttgcaa
    18061 tggatccttg acggtgcgaa aaaagtcatc gccgcagact ttcatttaac ggttccacaa
    18121 gttgtcgcta atgcaatttc tgaatataag acggcgaatg attggcttgg ccatttctta
    18181 gacgagtgct gcgagactgc tgttgacttt gaagaaaaat ctggtgagtt gtatgcagag
    18241 tatcgtgcgt tttgtacgcg aactggtgag tacattcgta gttcagccga tttttacacg
    18301 gctctggagg cgggcggatt taagcgccgg cgaactaata aaggaaacaa gattgttggt
    18361 ttgcaactca aatccgagtt cttggaatag ttgattttaa aggaaagtgt aacctgtgta
    18421 ggtcttatat agaacttttc ttaagggata aaaaattacc acctatataa agttatgtat
    18481 tgcgtattca cgggttacac catacttgca gattggagaa tatctaaatg aaatttaaac
    18541 agtggttaca aattggaatc ggtaaacgtg atttacgatt tgatgagttt tacgagtttg
    18601 ccatgtcgga tgtcacgtat ccttggaaga aatcattcga taagcaagtc gcacatctga
    18661 tgattcactg cgcagaaaag gaatacttgg aaatcttgcg tgatgcgtat ttcgagtatt
    18721 tgacggtgtg gctatgagag aaaagcaggt tgagcaagca ctagtgaaag ccgtcaaaag
    18781 ggtaggtggt atttgcccga agtttacatc accggggctt gctggtgtgc ctgaccgatt
    18841 ggtacttatg ccaaatggga aacttggatt tgtcgaagtg aaggcgccag ggaagaagcc
    18901 acggtcttta cagctttttc gtatgaaaca actgacagat ttagggtttc agtgtttcgt
    18961 gcttgatgag attgatcaga tacctgagtt actagaaaga ataggaggtg atgtctgatg
    19021 aagttcgtac cacataacta ccagaaatat gcgattgatt ttatcaaaga tcataaaact
    19081 gccgcccttc tattggacat gggcttgggt aaaacggtga caacgctgac agctattaaa
    19141 gacttgatgc atgatgactt tacaatacaa cgtgtcttaa ttattgcacc gttgcgcgtg
    19201 acgcaatcaa catggccaac tgaaattcaa aagtgggatc atttgaaaga tttatcgtat
    19261 tccgtggttc ttggaacacc aagtcagcga atggcagcat tacaaaataa tgccaacatc
    19321 tatctgatca atcgtgagaa cttggactgg ctgattaaca aatcgggttt tacgtttgat
    19381 tttgatatgg tggtgattga cgaactgtcg tccttcaaaa acttaaaagc taaacggttc
    19441 actagcttta tgaaagtcag acacaaggtt gaccgaattg ttggattgac cggaacacct
    19501 tcaagtaatg gtttgatgga tttgtttgcg gagttcaagg ttctggatat gggcgagaga
    19561 ttgggctatt atatttcacg ttatcgagat aaatactttc tacctgacaa gcaaaatggc
    19621 atgcagattt attcttggaa accaagggaa gatgcagaac aagaaatcta cgacaagatt
    19681 tcagatatca cgatttcaat gaagtcagtc gatttcttgg atatgccaga gcttgtgatg
    19741 aatgaagtgc cagttgatat gggactcaaa gaaaagcaaa agtacgatga aattaaggca
    19801 gacttggtgc tacaattgaa agaagccacc attgatgcag ccaatacagc ggtgctttcg
    19861 aataaactgc tacaaatggc gaatggagca atctatgatg agtttaatgt gagtcatcac
    19921 atccatgacc agaagttaga tgctttggaa gatttgattg aaggtacaaa tggcaaaccg
    19981 attctgattg cctattggtt ccagcatgat ctggagcgta tcaaggaacg attcaacgtt
    20041 cgacagatca aaacgtctca ggacattatt gattggaacg aaggtagcat ccccatcgct
    20101 gtcattcatc cggctagtgc aggacatgga ttgaatttac aagctggtgg ttcaacactt
    20161 gtatggtttg gtcttacgtg gagtttggaa ctttaccaac aaaccaatgc aaggctgtgg
    20221 cgacaagggc aaagtgatac tgtggtcatt catcacatta ttgcgaaaga tacgattgat
    20281 gaagatgtga tgttggcttt gaaacgaaaa gataaaacac aatcatgttt gattgatgca
    20341 gtgaaagcta gattggaggg aagcaagtga caccaaaaga atacttgaaa caagcgtacc
    20401 gtttgaataa acgaattgac gaccggatta aagagaaggc ggagctctat caactggcgg
    20461 tgacagtttc ttccacaaca ttcgaagaaa agatttcaag tggcacacgg aatactgagg
    20521 caccatttgt tcgtaacctt gagaagtgtt accaattgga agctgaaatt gatgcagaga
    20581 ttgataagtt tgtagacctc aaacgacaaa tgcgtggcgt gattgatgaa ctcacaaacg
    20641 tggacgaaaa gattgtgtta atcaatcgat acctgttgga agagagctgg gacaagattg
    20701 ctgaagatct tcacgctgac gtgcggacag tctttcgttg gcatagcaaa gctcttgaga
    20761 acttgaagct accagatgaa cccatagtca tttgaaatgt gtcagtgaat gtcagtagcc
    20821 tttagtgata tcattaagat gtcaaaaaag acaaacgata ctcaagcaat accagcaata
    20881 gaagtatcga acaaatactc gaagcctttg tgggaaaaat ccttcaaggg cttttctttt
    20941 gcccgaaagg atgtgacccc acttgccaat gaaaccagct aacccctgta agttcccagg
    21001 ttgtccgaac cgtacgcatg atacttattg tgatgaacat aaaacacttg cgaggaaacg
    21061 ttacgagaaa tacgagcgtg atccggcaat caacaaacgt tacggcagag cgtggaagaa
    21121 aatacgggca aggtacgtgg cagcacatcc cctatgtgag atgtgtctga aggaaggacg
    21181 ctttacttcg gttgagattg tgcatcacat caaggaatta agtgatggtg gtacgcatga
    21241 tgaaaataat ttgatgagtg tctgcaaatc atgtcactcg aaaattcaca tgactcgacg
    21301 aaacacaaaa gataaaacaa tcatgtgaaa aataacaagg gggggagggg cggtaattat
    21361 ccctaaaccc ttctaaactg tacagcgcgc taggccctta cgcgaaaaaa gttcagttca
    21421 aacgggggat agccccaaac aagaaaaaga ggtgataaat tttggcaaaa gatggaacac
    21481 aacgcggtgg cagacgacct cgttcagggg aacgctcaca gccactcgtt gataaagttg
    21541 ctgctggaaa gaaagcatcg cggatggaat ttgacttacc ggatgttgaa ttattaattg
    21601 gtgaagatat tggtgagggt gtagagcttg agggcaatga ttatcctgaa ccaagtgctt
    21661 acctttctgc aaaacagaag gatggcactg tgctaggcgc tgatgaaatt tataactcaa
    21721 cgttccaatg gttgaaagac cgcggatgcg aaacgttgat agccccacga atggttgagt
    21781 cctacgctca gaactttgcg cgttttgttc aatgtgaaaa tgctctgagt caatatggtt
    21841 tgctcggaaa acacccgact accaaggcag tcatggcctc cccatttgtg gcgatgtcgc
    21901 agaacttcca aaagcaagca aatgtgacgt ggtatgagat tcaacagatt gtaaaagaga
    21961 atgcatcagt ggaatacaca ggagatccga atacggatgc gatggaacga ttgttacgtt
    22021 ctaagaatgg ttgaaaggaa cagaatttat gatagagaag gttaatccgc aacatccaga
    22081 taaagttgca gatcgtattg ccggagccat tgtggacttg gcatacgcag aagaaaataa
    22141 tccaaagata gcagttgagg ttttaattgg tcatggcatc tgtcatgtga ttattgaaac
    22201 ttcaactgct ttaaatttga atgaaatcag ccaagcgatt aatcgtatcg caggcaaaat
    22261 taaaaaagat gtggtgattg tgccccaaga taaagagttg gctgacaatc aagcgactga
    22321 aattcgttgt ggcgataatg gaatctttcg tgggatgcca ttgactgatg aacagttaca
    22381 gctctcaaaa attgcgcgtg acttatatca ggaccatcca tttgatggaa agtacattct
    22441 tgatggtgaa cgcttgataa tttgtcagag ccacgcaaag acaaatgagt taaaacaaaa
    22501 atatccaaac gctacagtca accctctagg tgattggact ggcggcccgg atgtggatac
    22561 aggtgcgact aatcgcaagc ttggttccga tatggcagac ggagtaacag gtggcggtat
    22621 ccatgggaaa gacttatcaa aagctgatgt gtcagtgaac atctacgcat tcttgaaagc
    22681 tcaagaaact ggtatgcccg ttgagttaag ctgtgcgatt ggtgacactg agattgatgg
    22741 cgtgccttat tctgaaatcg tggaagttgc gcgtgattat gtgaatgagc ttggcgggtt
    22801 tgagaaacta gcagaatggg ggttgttcta atgaacacaa cagaacgttt tgagaaagtt
    22861 gatattgata agctggtccc ttatgccaga aatgcccgta cccacagtaa ggatcaagtg
    22921 aaacagattc aagcttctat gcgtgagttt gggttcataa atccggtgat tgttgataag
    22981 gattataact tgattgcagg tcacggacgt attcttgcgg ctaaagaaga aggtatcaag
    23041 caggtaccgt gcgtgtttgt ggaacaccta accgaggcac aaaagcgtgc gtacatactt
    23101 gctgataacc gactagcaat gaacgccggt tgggatgatg aaatgttggc ggttgagtta
    23161 tcagacctgc aaggtgaaga gtttgatttg gatttgcttg gttttgaaac tgatgaactt
    23221 gataaattac tcaacggtaa tgatgatgcc caagatgatg actttgatgt agatgaggaa
    23281 cttgaaaagc cagccttcag taaaacaggg gacttgtggt tgcttggtcg tcaccgttta
    23341 gtttgtggtg actcaacaaa acctgaaact tatgagaaac tcatggacgg caaactagca
    23401 aatcttgtgg tgacagatcc accttatggg gtctcatatc aaggtaaagc gggaacgatt
    23461 aaaaatgata atctgaagga cgaggcattt tacaacttct tgtatgatgc cttttcttgt
    23521 atggaaagtg tgatggccaa cgacgccagt atttatgttt tccatgcgga tacggaaggc
    23581 ttgaacttca gaaaagcatt cgtggatgca gggttctatt tatcaggtac gtgcatctgg
    23641 aagaaacaga gtctagtgct tggtcgttcg ccttaccaat ggcagcatga gccggtgctc
    23701 tatggctgga agaagaaagg caaacatcaa tggtacaccg gacgtaagga atcgaccatc
    23761 tgggaatttg ataaaccaaa gaagaatggt gaccacccaa cgatgaaacc aattccgctg
    23821 ttggcatatc cgattaccaa ttcgtcactc agcaactgca tcgtgttgga tagttttggt
    23881 ggcagcggat caactttgat tgcttgcgaa cagaccgacc ggatttgcca tacgatcgaa
    23941 ctggatgaaa agttctgtga tgtgattgtt aagcgttata tcgagcaggc tggaagcgat
    24001 gcagacgttt cagtgattcg agatggaaag actatcgcat ttaatgacct agaaaatcct
    24061 gaataaataa cgtatcactt gctataaaat ccctttagag tgatatatag actaccaaaa
    24121 gaatacggaa gacaaagtta tgtttccaac tcatgaacaa atcgagggta tgaaaaaacg
    24181 ttaccctgaa ggtagtcgtg tagaactcat caagatgaac gacccacatg ccccagcgaa
    24241 gggcacacaa ggcacagtaa ccggtgttga cgacattggt tcaatcctta tccaatggga
    24301 caacggttca acactgaacg cggtatacgg cgaagatgtg gtgcacatca tcaaaccaga
    24361 cttcgaattg gtctttcaaa acggtagaag agaaggattt gaaaccttca atgatgcttg
    24421 gcaatacgtg acggacatga tattgaacca cgatttggtc tgggttgacc ttgagtcaaa
    24481 aacagaagat accatctaca tcagaaaggg gctttaagat gaaagtgagt aaacgtaaga
    24541 tttacaacat cgcaaagaat tacatcgttc agttgcctga acgtggcgac ctgaaggccc
    24601 acaacagcga ccgtgaagac ttcttggaca ttgcggtttg gtgtttggaa gatgccttaa
    24661 ttgcggccta tgaggcaggc agaaaggacg gtcaaaatga acgcaaagat taagcaacaa
    24721 atcattgaga ttgcagaaag tggtctgaca aatatgtttg atgcgattgc agtgcagcga
    24781 attgcacatg accggcactt ttacgagttg gtgattttca ttgaagaaca caaagatgag
    24841 tacagccgct ttatcttgga gggtgataaa tgatggatta tcaaatttac atgccagata
    24901 atgaagatgg cattgaagaa ggatactacg attggcaaga actggtgcaa ttgctgcggg
    24961 ataacaagaa caaccctgaa gccattcagt tcattgccga tatgatggaa taaagagatt
    25021 aaggcagctg acaacagctg ctttttgcgt agaagggagt gagttccaat cgataattac
    25081 aaaccaactg aatttatgga caaagactcc cattatgata agtcacgtgc agattatgcc
    25141 gtggccttta ttgagttgtt gaaacacacg aaaggtcagt ggttcgggaa accgttcaaa
    25201 ctgatgcctt ggcaagaaaa gattatccgt gacttattcg gaacgattaa ggcaaatggc
    25261 tacagacaat tcaatacggc ttatgttgaa atacctaaga agaatggtaa gagtgagtta
    25321 gcggctgccg tggctttgct tttgacttgc ggtgatgggg aagaacgcgc tgaggtatat
    25381 ggctgtgctg ctgatcgagc gcaggcttca atcgtatttg acgtagctgt tgatatgatt
    25441 cgccagaacc cagcgttaag caaacgagtg aaaatcctga cttcacaaaa acgaattatt
    25501 tatcagccaa cgaatagttt ctatcaagta ttatcagctg aagcatactc taaacacgga
    25561 ttaaacagtt ctgcggttgt atttgatgag cttcacgcac aacctaacag aaagctgttt
    25621 gatgtcatga cgaaaggggc aggggatgct agaacgcaac cgctattctt tttgattaca
    25681 acggcaggga atgataccaa ttcaatttgt tatgagcagc atcaaaaagc tctcgacatt
    25741 attgaaggtc gaaagcatga taaaacattc tatccgttaa tttacggtat caagcaagat
    25801 gatgattgga ctgatccaga agtttggaag aaagccaatc cgtcacttgg gataacgatt
    25861 gcagaagaaa aggtggtggc tgcttgtgat tcggctaaac aaaatccagc tgaagaaaac
    25921 tcattcagac agcttcgcct gaaccaatgg gtgaaacaag cagtgcgttg gatgccaatg
    25981 gataaatggg acgcttgttc attcaatgca gatgaagaaa gtttgagagg tcgagtttgt
    26041 tacggaggtc tcgacctttc cagcacgacc gaccttacat ccttcgtttt ggtgtttcca
    26101 cctgaagatg aggatgacaa gtttgtgata ctgccatatt actgggtgcc agaagatacc
    26161 ttggacttac gagttaaacg cgaccatgtg aattatgatt tgtgggccaa gcaggggtac
    26221 atacaaacca ctgagggaaa cgtagtccac tatggtttca ttgaaaaatt tattgaggaa
    26281 ctgggtgaga aatacaatat ccgggagatt gcctttgacc gttggggagc agttcaaatg
    26341 gttcaaaact tagaaggtat gggatttact gtagttcctt tcggtcaagg tttcaaggat
    26401 atgagcccgg ctactaaaga gttgatgaag ttgactcttg aacagaaatt agcccatggc
    26461 ggtcatccgg ttcttcgttg gaatatggac aacattttcg tccgaactga cccggcaggt
    26521 aatatcaaag ctgataaaga aaaatccact gagaagattg atggtgccat cgcgacaatt
    26581 atggctctgg atcgtgcaat tcgttgtgga aatgataatg gtgccagtgt ttatgatgat
    26641 aggggattgt tgtttctgtg aaatatgagc ttttattaca atccattgat ataattgtct
    26701 ttggaggtgt atgtgtatga atgaatattt gattcaagta gcaccagata aaatatcttc
    26761 agacccggac aacacattaa tttctagact gttttataga aaatggtggg gaaaaattct
    26821 aactgaagat ttgacaaagt atgctgaagg aattgggaat tataggggag aaacgattat
    26881 ttctttcaac acaattgctg gtgcaacgat acggttattg aaatgctata atcaaaagaa
    26941 cttttcaatg ccagatagtc attatgggcg tttgaaagtc attttatatt ccgatgatat
    27001 caccgaagag atgaaagtga aatttgtaaa attctatgat ttaagtcata ctcttgcaaa
    27061 ttttatgcca ctatatgtat caaaaccttc tctcaatggt gttaaaggta aagggccgta
    27121 tcatgacttt cctgatttat tttattctga tgtaagagat aaacgatatg gttcatcaac
    27181 taacgctgat aaatttatcg ttaataataa agactatttc tctaaattca aagattggaa
    27241 aagttttata gaactaaatt atttacaaga tttcttcgat gatgaagatt atatgacgtt
    27301 taaaaaactt gcaccaagta gttcattctt tccttatcaa agaaaatcct taacacctga
    27361 cgaaaaatcg aaatgtttag aggaagtaaa taccttttta aataatgcaa ttgagataat
    27421 tttgtcaaga ggaagtcggt tttatatcta atgtgaacac agcatctaca aatcgtaggt
    27481 gcttttttga tactcaaatt ttgaaaggag caatcaatgg gcataataaa atcactattc
    27541 aaatcacgcg ataaaccaac aaatcaaacc atcatgaatc cataccgatt tatgtttggc
    27601 ggctcatcag ctggtaaaag cgtgaccgaa caaacagcta tgcagatgac ggctgtgtac
    27661 tcatgtgtgc gcgtgctatc agaagccgtg gcaggattgc cattacactt ttaccaatac
    27721 cgagatgctg gcggcaaaga taaagcaatg aaacattcgt tgtatttctt gctgcatgat
    27781 gaaccaaatt cggaaatgac atcatttgta tttcgtgaaa ccttaatgac tcacctgcta
    27841 ctttggggta acgcctatgc gcaaattatc cgaaatggca aaggtgaagt tgtagctttg
    27901 tatcctttaa tgccagataa gatgacggtt aatcgcgatg acaacaagga aatctattat
    27961 ttatatcaag ctgatgtcgg gcaagtcaga ctttcaaaag ctgacgtgct tcacattcct
    28021 ggccttggct ttgatggctt ggttggttat tcaccaattg ccatggctaa gaatgctatc
    28081 gggcttgcga ttgcaacaga ggaatatggt tctaaatttt ttgccaatgg tgccaatcct
    28141 ggtggtgtcc tagaacatcc tggaactttg aaagacccag gaaagcttcg tgaaagttgg
    28201 aatgcgacat ttggtggcag ttcaaatgcg gccaaggttg cagtacttga agaaggcatg
    28261 aagtatacgc ctatttccat gtcaccagaa caagcccagt tccttgaaac acgtaagttt
    28321 caactggaag aaatcgcacg gatattccga gtgccccttc acatggttgg cgacttagac
    28381 cacgcgacct tctcaaacgt tgaacatttg agtttggatt ttgtgaagtt ctcattatca
    28441 ccatggctca atcgttggga acaagcactt aataaatcac tgttaagtcg cgatgaaaaa
    28501 gaaaagtatt tcataaagtt taatgtggat ggcttattgc gtggtgacta tgccacacgt
    28561 caacaaggct atgcaaccgg tattcaaaat gggttcctat ctcctgatga tgtgcgtgaa
    28621 cttgaggact tgaacccaat ccctggtggt gctggtgcga aatattactt caatggcaat
    28681 atgctgccaa ttgaacaagc cggggcagct tatggcgtaa acaaagaaga aagcgaggaa
    28741 caagatgaag aagttttgga agtggaagaa tcaaacggtt ctgaatcaag acaaccaaga
    28801 ggaaatccta gagagaaccc tctacctcaa cggaccaatc gctgaggaga gctggtttga
    28861 tgatgaagtc acaccgcaga ttttcaaaga tgagttaaat tcaggtggag gtaacgttac
    28921 agtctggatt aattcgggtg gtggcgattg tgtcgctgcc gcaagtattt acaacatgct
    28981 tgctgactac aaaggtaaag tcacagtcaa gattgacggc atcgccgctt cggccgcaag
    29041 tgttattgca atggcaggtg acaaggtact catgagtcct gtggcgatgg tgatgattca
    29101 caatccgtca gcggttgcgt ttggcgaaaa agttgatatg gagaaagcca ttgccatgct
    29161 tgatgaagtg aaagcaagta ttatcaacgc ctatgaacgt aaaacgaatt tatcgcgtgt
    29221 gcaactggca catatgatgg atgcggagac gtggatggac gcaaataaag ctgttcacct
    29281 aggatttgcc gatgaaatca tcggtgagat tgacccggat attccggtta ataattcgat
    29341 gtactcggaa gtcaaagtaa ctaattcact gacttcagcg attgctaaga agtgtcgtat
    29401 tgaaccacct gaaccaaagg aacgagaaga aattaataac actatcccag ccgattcgtt
    29461 aatgagtcgg cttaatttaa tgaaaaattg gaggaactaa cccatgaaca aaattcaaga
    29521 attgatggaa aaacgtgcac aagcttggaa cggagcaaag gcttttgtag aagcaaagca
    29581 agataaagat ggcttgatgt ctgaagaaga tgccaagact tatgcggaaa tggaaacaaa
    29641 agtgaagaac tttacgatgg aaattgaacg tatgcagtca atggaagcga tggatcgtga
    29701 acttgaaaag ccaatgagcc aaccattaac ttcgcaacct atgactactg aaaataaaat
    29761 ggaacctaag aaaacaggac gtgcttcagc tgaatataaa gaaggtgtac ttgaagcctt
    29821 gcgctcaaac ttccgacatg tttcaaatgt tcttcaagaa ggtgtggata cggcaggcgg
    29881 ttatttggtg ccagaagaat atgactcacg tttgattgac ggcatgactt ctgaaaacat
    29941 catgcgtaca cttgcgacaa caattacgac aagtggtgaa cacaagatta atattgcagg
    30001 ttctaaacca gccgcttcat ggattgaaga aggtggcgag ttgagctttg gggatgcaac
    30061 gttcgatcaa atcttgcttg atgctcacaa acttcatgtg gcaatcaagg tgactgatga
    30121 attgctttat gacaacgcct ttaaccttga aaactatatc atcgaccaat tcaccaaagc
    30181 cttagcaaac gcggaagaag atgcgttctt gaatggtgac ggtgttggta aaccactcgg
    30241 tatttttgca gcaacaggtg gcggacaagt tgccattaca actaatactc aaagtgcagt
    30301 tacggcagat gatgtggtga accttgtgta tgctttgaaa cgtccatatc gtaaaaacgc
    30361 gaagtttatc atgaacgacc aaaccatcag cttgcttcgt aagttgaaag acaacaatgg
    30421 tgcctacatg tggcagccag ctttggtagc aggtgaacct gaccgtctgc tcggttatcc
    30481 agtttatact tcggagtttg ttccaaccgt taaggcaggt gcgccagtca ttgcttttgg
    30541 tgatttcagc tactataaca ttggcgaccg tggcgtgcgt tcattcgacc agttgcgtga
    30601 actctttgca ggtaatggca tggttggttt cttagctaaa gaacgtgtgg acggtaaact
    30661 tgtgttacct gaagcagtgc aaatcttgaa gatgaaagca tctgcctcat cttaaggagt
    30721 agcctatgag tgacttacta aataaggtca aggcaaacct catacttgag cataacgaag
    30781 atgatgagct gttggaacga ttgattctga ccgctctttc ttatgctgag agctatcaac
    30841 atattgagtc tggctactat tcagaacatg aaatgccgcc aaccactgaa caggcggtta
    30901 ttgtgttagc cagtaacttt tacgaaagcc gtgatggttc aactggtggc ttctttgcgg
    30961 ataacgtcaa tgccagtaag caagtctggg aaacggtcaa tctgttactc aggctcgata
    31021 ggcggtggca agtatgagtt ttggaaagat gaatcaacga atacaaatcg ttaaagtgac
    31081 gaaccaaaag gatagcgctg gattttcagt gaaacaagat gaagttcttg cagctgtcca
    31141 tgcgtacaaa gaggaaaaga atgcgactga gaaatgggta aacatggcga cttttagttc
    31201 agcgacctgc ctgtttcgga ttcgttatat tcctaatcta gaggttacga cagagatggt
    31261 tgtcatttca gatcagaaaa ggtatcagat tgtgtcggtg gagaatgtcc gaaatcgcaa
    31321 tatgtacctt gaaatgctga ctactgaaat ggaggtgagt gcggatggct aaagcattga
    31381 tgaagatgcc agaagatttc ttgttaaagg tttcaaaact agaaaacaag acagatgaaa
    31441 tcatccccaa ggttttagaa agtggtgctg aagtggtgga aggcaaagtc cgaagtaact
    31501 taactgccgt tgttggctca aatacaaaag taccaagtcg ttcaactggt gaacttgaat
    31561 cagcccttgg tatcacacaa gctcgtcaag ataaaagcgg caactggaac atcaaggttg
    31621 gttttgatga gcctcgaatc gatggcagtt caaatgctaa gattgcgaac attctcgaat
    31681 atggaaaaca cggtcagcca gcaaagccat tcttgaaacc tgcaaaatca caaagtcgca
    31741 agtcatgcat tgaaacaatg aaatcaaaaa tggaaagtga ggtaaacggc atttgaacat
    31801 tttagaggaa ttgaacagtc tttttgccac acttcatatt cctgttgaaa cgggcgagtt
    31861 tagtgataaa gcgccagaca agtattgtgt gctgacacca ctcgctgaca ggttcgatgt
    31921 ttacggggat aactttccaa ccgttgatgt acaggaagtt cgcatttcct tgtttgtgaa
    31981 aggcaactat ctgaacacgc aaaaacaact ggttcagaac ttgttgaaac acgagtttac
    32041 gattactgaa cgattatttg taggatttga aaaagataca aattactatc atgtggcgat
    32101 tgatgtcgcc aagcattatg aaatggaggt ctgaaaatgg caacgattgg attggataaa
    32161 ttatattatg caccgattac agagtcggca actactggcg aagaaacata cggcacaccc
    32221 gctatgcttg cgaaagcaat ctcagctgag ctttcggtgg aacttgctga agcaacttta
    32281 tacgccgatg atggtgcatc agaagtagtg aaagaattta aaaatggaaa gttaacgctc
    32341 ggtgttgatg atattggacg tgctgtggcg gcagtgcttg ttggtgcaac cgtggatcaa
    32401 aacggtgtct taatttcgac aaccgaagat ggtggcacac cagttgcgat tggtttccgt
    32461 gctcgtaaat cgaatggcaa atacaaatat ttgtggttgt accgagtgaa gtttgccgtg
    32521 cccagcacaa gcctagcaac aaaaggtgac gacattacat tctcaacacc aacaattgag
    32581 ggtacagtca tgcgtcgtaa caagttagat gctagtggta agcatccatg gaaagcagaa
    32641 gttgatgaaa acgatacggg agttgaacaa acaactatca atggatggta cacgagtgtt
    32701 tatgaaccag atttcacagc agactaggag gcaacaatga gtgaagaacg cagtgccaag
    32761 attagccttg gcggagatga atatgaactg attttgacaa caaaagccac gaaagaaatc
    32821 gccagtcact acggtgggct tgataacctt ggtgagaaat tgttgaaatc cgagaacttt
    32881 gaattggcgc ttgatgaaat catctggttg attacgctgt tggcgaatca gtccatcaag
    32941 atttacaact tgaagaacac agaaaagaaa gatgaactca cgactgaata tgtggagttg
    33001 atgacgagtc cgttgcagtt ggctgaatat aaatcagcaa tcactgaagc aatgttcaag
    33061 ggcacagctc gcaatatcga aagtgaaaca gattcaaaaa acaaagcagg cgagtaagtg
    33121 atgaagaaac atttactcgc ctgtattact atgggacagt tcaaatgggt atgacgtcag
    33181 atgatttctg gtactgctcg cttggactgt ttttggattt atgggagtgt cataagcaat
    33241 ttactgggat tagcaagccg aaagttgagt tattcattga tgatgtgata ccgagtggaa
    33301 tttaagggaa ggtggtgatt tatttggcag ataattttgg tttaaagatt ggcgttgaag
    33361 gtgaaaagga gtttaagaac tcgttgcgtg aaatcaacca atcattcaag gtgctaggtt
    33421 cagaaatgaa actggtatca tccgaatttg ataaaaacga taaaagcatc agctcactgt
    33481 ctgctcgcaa ccaagtctta aacaaatcga ttgatgccca gaaagaaaaa atcaccaccc
    33541 ttgaaaatgc tttgaagaac gctagtgaca gttttggtga aaacgataag cgtactcaga
    33601 attggacagt gcaattaaac aatgcaaaag cccaactcaa taatatggag cgtgaagttg
    33661 ctaacaacga gaaggcaatt gacgccatgg gtactgaaga agttgatgct gctaagaaaa
    33721 ctgatgagct tggcaacgaa ctcaaggata caggtgacga ggctgaaaag tctggaagta
    33781 aatttgaaaa gttcggtggc gttctaaaag gtatcggggt agcaatgggt acagtggcac
    33841 ttgctgctgg cgctgctgcc gtggagctag gaaaacaagt ggtatctgct tacgcggact
    33901 atgaacaatt agtcggtggg gtggatactc ttttcaagga tgcgtcatcc accgttcaaa
    33961 agaatgccga caatgctttc aaaactgccg gaatgtcagc caatgaatac atggaaactg
    34021 tcacaggttt ctcagctgcc ttgatttcat ctctcggtgg tgataccaag aaagccgcag
    34081 gagttgcgga tcaagctgtt actgatatga gcgataatgc aaacaagatg gggacggata
    34141 ttgagtccat ccagaacgcc tatcaaggtt tcgccaagca aaattacggg atgctggaca
    34201 acttgaaatt aggttttggg ggcacgaagt ccgaaatgga acgtttgctc caagaggctg
    34261 agaagatttc tggtgtcaaa tatgatatct cgaacttcgc tgatgtgact gaagcgattc
    34321 acgttattca aaccgaaatg ggcatcactg gtacaaccgc caaggaagcg agtgagacga
    34381 taagtggttc gattgacggt atgaagtctg caatctcaaa cttgatggct ggacttggta
    34441 acagcagcgc tgacattggt atgttgattg gcaatgtggt ggaacaattt caaaacgtca
    34501 tcaagaatat tctaccggtc attcaaaata tcgtgtcggc tttaccggca gtggtggatg
    34561 ccattattca agcattgggg cagttattgc caacactctt agaagcagta acctcattat
    34621 tctcacaagt gcttgaaacg atacttggct tacttcccac acttattcca gtggtggttc
    34681 aagccgtttt aacgattgtt caaacactta ttgagaactt acctttactg attgacgctg
    34741 cctttcaact tgtgacaaca cttatccaag gactaggcga agcgctgcca gaacttattc
    34801 caattacagt acaggcaatc ataacgattg tgaacggctt gattgaaaac ttgccattac
    34861 tgttggatgc tgctttacaa cttatcatgg gacttgccca aggacttatc acagcacttc
    34921 caatactgat tgaaagtctg ccacagatta ttacaggaat cgtgaacttt attatcagtg
    34981 ctattccaca aatcattcag actggtattc aacttttgac atcacttgtg acagctttgc
    35041 cggatatcat caatgcgatt gttgcagcta ttccagtgat tattcaaaat atcatcaatg
    35101 ccgtgattgg ggcaatccct cagttgattg acgcaggtat tcagttactt gtggcattga
    35161 ttggtgcgtt accgcaaatt atcacaacga ttgcgaatgc catgccgcaa attatcaacg
    35221 gaataacagg aactttggta aacaacatcg acaaaatcat caatgctggt gtgaagttgc
    35281 ttgtggcatt agttcaaaac ttaccagcaa tcatttctgc cattgtcaaa gcagtaccgc
    35341 aaatcatcac agggcttgcg aatgctttcg gtgagtatgt ttcaactatg ggaactatcg
    35401 ggaagaactt gattacagga ttgtggaatg gtatcgctga tgctggaagt tggttacgca
    35461 acaagatttc aggattcttt ggcggtgtag tagacaacat caaggacttc tttggtattc
    35521 attcaccatc aacactgttt cgtgatgaaa tcgggaagta catggcacaa gggattggcg
    35581 taggttttga taaggaaatg actggtgttt ctaaaacgat gcagaaagct attccaacaa
    35641 aactttatat gccaagcttt gatattgata ccgatatcca cgctgccgtt gatagcacat
    35701 tctcgatgct tgatttaggt gctaaggttg atgccttaac aaacatcacg agtgaaatgt
    35761 tgccaacgct acttaaagca atggacatta aagtcgtctt gaatgatgga acattggtcg
    35821 gcaaacttgc tccagaaatt gataagagct tggcgacgtt gaatcgtaga cgtgtagcat
    35881 attagtgttg gattttcaca ttttgactgt atactataac taacgagtgt ttactgaaga
    35941 gatggtggta atcatatgtt gaagcataaa tttaaagcaa agaaacctgc actgatagga
    36001 tatattctac tgcttgctat tggattattt ttaacggcag taagatggta tagcggcata
    36061 aatcatggtt ttgtattcat caacaatcag attaattctc acatttcaaa cttcgcatta
    36121 agctttcttt tctatttggt gataggctat ctttggttgc ttatgggaac gaatttaagg
    36181 gttcttactg cgatgggagc tggtctgatt gttgcaaata ttgtatgtga aacgatgatg
    36241 acttttctga atacaccaga tctaatcgat gcaatatatg gaatttccgg cgtgctggtt
    36301 gtatacgcgt atttagcgat taccaagaaa atagggctgg aagaaacaac cgaataggat
    36361 gatagattat aaactttagg attagacatt gttgtctggt ccttttttag ttatttagat
    36421 gggcggtggt caattgaacg catttatttt agacggaaca attaattcac ggactgattt
    36481 aggtttgcga attacggaag tgccagttat tccagtgacg gaacgtgtca ttgatagtat
    36541 tgagattgat ggacgtgaag gagatttgac cattctgaaa ggctggaaag atgtgacttt
    36601 cggttttaag gtagtgattt ggaaatctga tgtttggact agctggcaga taattttgcc
    36661 acaaatccta aacgctaaaa ctatcgcatt ttcaaatgaa ccaaacgtgt tttacccaat
    36721 caagtatgct aaggcgagcg gactaactca gattttatct tccatgtggg agtttgaaat
    36781 ggagttaacc tgttcgcctt ttcgttataa gaccaatgta gcgactatca atcgaaccac
    36841 gtcaggaacg gtcaataatc ccggcaacat ttactcattg ccgaagatta aggtctacgg
    36901 caatggaact aggacactca cgataaatgg caagcctatc gtgttaaatc tgacgaatgg
    36961 caacctgaca ctagattcgg atttgaaaga atgcttttac ggggatgtgg cagccaatca
    37021 atacatgaca ggtgactttc ctgagtttcg agtcggcagt aatactgtga ctcttggtac
    37081 aggtataacc aaagtagaaa tagaaccgag gtggcgatac ttatgattac gctttacaac
    37141 aaaacagaat caaatttcaa tcataacggt ctcgctgtcc ttgatggcaa cattctgaac
    37201 cccgtggtga gtgaggaatt gaacggactc ttttcctttg aattcgatta tccaattcat
    37261 gctccacaca gtgaggaatt gttgccagaa atgattgtga aagcaccagt accagaactg
    37321 tccgaccagc ttttcaggat tgttgaacga gatgatgcac ttggtggttt tttgcacata
    37381 gtagctcatc atattttcta tgacctagcc acaaacttta ttgaagatac ttatatcgtc
    37441 aacaagaacg ggtcgggagc tttgacacaa ctgcttggtg caactcaatt catgcaccgt
    37501 tttaccggta catcaaacat cagtgcggtc aataatgtcc gcttggtgcg actaaatcct
    37561 gtagaggtgt tgcttgatag tgacctagag aatggttttc aaagcagtta tggtggtgaa
    37621 attgtacgcg ataacttcac gatttcaatg ttaacgactc gtggcagtaa caacggcgta
    37681 caaatccgag acaagaagaa tctgacagga tataaagctg atgttgatta ctctggaatt
    37741 gttactcgca ttatgccaca agggtatgat ggactatttc ttcccgaaaa atacgtggac
    37801 agtccaaaca tcaaccaata cgtccagcct aaaatcaaag tcatcaagta cgacaacgtc
    37861 aaagttggtg atgaagagga tgagtttact caagccgagg cctatgatga acttcgcaga
    37921 ttggctgggt tagaatactc agctaacaag gtcgatttgc caaaagccac ctatgatgtg
    37981 gagtttgccc cgcttgagcg aactgaagaa tataaagatt ttgcacagct tgaaacgatt
    38041 aatttgggtg atactgtttc agttattcat gccgaagatg gattgaacat cacagctaga
    38101 atggtctcat acaaatacga tccatttctg aaagcgtata tctcagtaac actcggaaac
    38161 atcacgccaa aattcacgga tgtggcgaag gacatcaacc gggtggacaa caaggcggag
    38221 caagccaaag acgatgccaa ttacgcattg agttcggcaa atggtaaaaa caccaacttc
    38281 tatggcccga caactccaaa taatccgaaa ctcggtgacg tttggtacaa agaaaatggt
    38341 gacaagtttg aaatgtgggt gtatgaaatc cgtgatggcg tcactcagtg gtatccactt
    38401 atgacagata tgacggcaga agaagtaaag caagccgtgg aacaagcaca aaaggatgcc
    38461 aatactgcca ctgaaaaggc caatcaagca tttaacgatg ctgtgaatgc tttgaatgca
    38521 gctgaaagtg taggtgcaag tgttgaaacg cttactacat ccgttgaaca gaatactagc
    38581 aagatttcat tagtggcgag tggtatgtca actcttgaag gacgagtaat cacagctgaa
    38641 tcaactctga cagtgcaagc tgggcagatt gctagtaagg caagtcaatc gacggttgat
    38701 aatctgacag ggcgtattac aactgcagaa agcaccatca cccaaaatgc tgatagattt
    38761 gagctatctc tttctaaaac aaataaagcg gtcaatgttt tagccggtat gagcattgca
    38821 gatttgcagt gggcagttgg tggtctgaat acaaatgatg gaaaagaaaa tacctatggt
    38881 ggatacgttc gctcaggttt tacaagagtt agaaatggcg aaaagtatct acttcaaaca
    38941 tttgtgggtg ggaaacttta ctctacttac gcaacggctt atctgcttta ttacaaagag
    39001 gataagtcct ttttatcgta cacccaattt ggaaacacca ccacaccgct tactgttcca
    39061 agcagtgccg cttttattcg catt
//
LOCUS       DAYYME010000008        38189 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000008 DAYYME010000000
VERSION     DAYYME010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.008"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            148..1164
                     /locus_tag="ACHBFW_000332"
     CDS             148..1164
                     /locus_tag="ACHBFW_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9316680.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            1305..2606
                     /locus_tag="ACHBFW_000333"
     CDS             1305..2606
                     /locus_tag="ACHBFW_000333"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFM9316681.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            2652..3314
                     /gene="deoC"
                     /locus_tag="ACHBFW_000334"
     CDS             2652..3314
                     /gene="deoC"
                     /locus_tag="ACHBFW_000334"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFM9316682.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            3335..3733
                     /locus_tag="ACHBFW_000335"
     CDS             3335..3733
                     /locus_tag="ACHBFW_000335"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFM9316683.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            3799..4881
                     /locus_tag="ACHBFW_000336"
     CDS             3799..4881
                     /locus_tag="ACHBFW_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9316684.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            5093..6655
                     /locus_tag="ACHBFW_000337"
     CDS             5093..6655
                     /locus_tag="ACHBFW_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9316685.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            6648..7781
                     /locus_tag="ACHBFW_000338"
     CDS             6648..7781
                     /locus_tag="ACHBFW_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9316686.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            7781..8737
                     /locus_tag="ACHBFW_000339"
     CDS             7781..8737
                     /locus_tag="ACHBFW_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9316687.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            8836..10002
                     /gene="deoB"
                     /locus_tag="ACHBFW_000340"
     CDS             8836..10002
                     /gene="deoB"
                     /locus_tag="ACHBFW_000340"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFM9316688.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            10026..10844
                     /locus_tag="ACHBFW_000341"
     CDS             10026..10844
                     /locus_tag="ACHBFW_000341"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM9316689.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            10861..11568
                     /gene="deoD"
                     /locus_tag="ACHBFW_000342"
     CDS             10861..11568
                     /gene="deoD"
                     /locus_tag="ACHBFW_000342"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM9316690.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(11866..12552)
                     /gene="gpmA"
                     /locus_tag="ACHBFW_000343"
     CDS             complement(11866..12552)
                     /gene="gpmA"
                     /locus_tag="ACHBFW_000343"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM9316691.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(12724..13305)
                     /locus_tag="ACHBFW_000344"
     CDS             complement(12724..13305)
                     /locus_tag="ACHBFW_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9316692.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(13438..14121)
                     /gene="rpiA"
                     /locus_tag="ACHBFW_000345"
     CDS             complement(13438..14121)
                     /gene="rpiA"
                     /locus_tag="ACHBFW_000345"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFM9316693.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            14440..14853
                     /gene="rpsL"
                     /locus_tag="ACHBFW_000346"
     CDS             14440..14853
                     /gene="rpsL"
                     /locus_tag="ACHBFW_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFM9316694.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            14937..15407
                     /gene="rpsG"
                     /locus_tag="ACHBFW_000347"
     CDS             14937..15407
                     /gene="rpsG"
                     /locus_tag="ACHBFW_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFM9316695.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            15501..17588
                     /gene="fusA"
                     /locus_tag="ACHBFW_000348"
     CDS             15501..17588
                     /gene="fusA"
                     /locus_tag="ACHBFW_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFM9316696.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            17760..18947
                     /gene="tuf"
                     /locus_tag="ACHBFW_000349"
     CDS             17760..18947
                     /gene="tuf"
                     /locus_tag="ACHBFW_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM9316697.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            19354..19662
                     /gene="rpsJ"
                     /locus_tag="ACHBFW_000350"
     CDS             19354..19662
                     /gene="rpsJ"
                     /locus_tag="ACHBFW_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFM9316698.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            19693..20322
                     /gene="rplC"
                     /locus_tag="ACHBFW_000351"
     CDS             19693..20322
                     /gene="rplC"
                     /locus_tag="ACHBFW_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFM9316699.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            20350..20973
                     /gene="rplD"
                     /locus_tag="ACHBFW_000352"
     CDS             20350..20973
                     /gene="rplD"
                     /locus_tag="ACHBFW_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFM9316700.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            20973..21263
                     /gene="rplW"
                     /locus_tag="ACHBFW_000353"
     CDS             20973..21263
                     /gene="rplW"
                     /locus_tag="ACHBFW_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFM9316701.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            21302..22135
                     /gene="rplB"
                     /locus_tag="ACHBFW_000354"
     CDS             21302..22135
                     /gene="rplB"
                     /locus_tag="ACHBFW_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFM9316702.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            22178..22456
                     /gene="rpsS"
                     /locus_tag="ACHBFW_000355"
     CDS             22178..22456
                     /gene="rpsS"
                     /locus_tag="ACHBFW_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFM9316703.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            22479..22826
                     /gene="rplV"
                     /locus_tag="ACHBFW_000356"
     CDS             22479..22826
                     /gene="rplV"
                     /locus_tag="ACHBFW_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFM9316704.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            22840..23496
                     /gene="rpsC"
                     /locus_tag="ACHBFW_000357"
     CDS             22840..23496
                     /gene="rpsC"
                     /locus_tag="ACHBFW_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFM9316705.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            23499..23933
                     /gene="rplP"
                     /locus_tag="ACHBFW_000358"
     CDS             23499..23933
                     /gene="rplP"
                     /locus_tag="ACHBFW_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFM9316706.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            23923..24111
                     /gene="rpmC"
                     /locus_tag="ACHBFW_000359"
     CDS             23923..24111
                     /gene="rpmC"
                     /locus_tag="ACHBFW_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFM9316707.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            24136..24402
                     /gene="rpsQ"
                     /locus_tag="ACHBFW_000360"
     CDS             24136..24402
                     /gene="rpsQ"
                     /locus_tag="ACHBFW_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFM9316708.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            24460..24828
                     /gene="rplN"
                     /locus_tag="ACHBFW_000361"
     CDS             24460..24828
                     /gene="rplN"
                     /locus_tag="ACHBFW_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFM9316709.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            24865..25173
                     /gene="rplX"
                     /locus_tag="ACHBFW_000362"
     CDS             24865..25173
                     /gene="rplX"
                     /locus_tag="ACHBFW_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFM9316710.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            25201..25740
                     /gene="rplE"
                     /locus_tag="ACHBFW_000363"
     CDS             25201..25740
                     /gene="rplE"
                     /locus_tag="ACHBFW_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFM9316711.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            25759..25944
                     /locus_tag="ACHBFW_000364"
     CDS             25759..25944
                     /locus_tag="ACHBFW_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFM9316712.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            25980..26378
                     /gene="rpsH"
                     /locus_tag="ACHBFW_000365"
     CDS             25980..26378
                     /gene="rpsH"
                     /locus_tag="ACHBFW_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFM9316713.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            26410..26946
                     /gene="rplF"
                     /locus_tag="ACHBFW_000366"
     CDS             26410..26946
                     /gene="rplF"
                     /locus_tag="ACHBFW_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFM9316714.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            27107..27463
                     /gene="rplR"
                     /locus_tag="ACHBFW_000367"
     CDS             27107..27463
                     /gene="rplR"
                     /locus_tag="ACHBFW_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFM9316715.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            27484..27984
                     /gene="rpsE"
                     /locus_tag="ACHBFW_000368"
     CDS             27484..27984
                     /gene="rpsE"
                     /locus_tag="ACHBFW_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFM9316716.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            27999..28178
                     /gene="rpmD"
                     /locus_tag="ACHBFW_000369"
     CDS             27999..28178
                     /gene="rpmD"
                     /locus_tag="ACHBFW_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFM9316717.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            28222..28662
                     /gene="rplO"
                     /locus_tag="ACHBFW_000370"
     CDS             28222..28662
                     /gene="rplO"
                     /locus_tag="ACHBFW_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFM9316718.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            28662..29957
                     /gene="secY"
                     /locus_tag="ACHBFW_000371"
     CDS             28662..29957
                     /gene="secY"
                     /locus_tag="ACHBFW_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFM9316719.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            30019..30666
                     /locus_tag="ACHBFW_000372"
     CDS             30019..30666
                     /locus_tag="ACHBFW_000372"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFM9316720.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            30857..31075
                     /gene="infA"
                     /locus_tag="ACHBFW_000373"
     CDS             30857..31075
                     /gene="infA"
                     /locus_tag="ACHBFW_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFM9316721.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            31108..31224
                     /gene="rpmJ"
                     /locus_tag="ACHBFW_000374"
     CDS             31108..31224
                     /gene="rpmJ"
                     /locus_tag="ACHBFW_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFM9316722.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            31242..31607
                     /gene="rpsM"
                     /locus_tag="ACHBFW_000375"
     CDS             31242..31607
                     /gene="rpsM"
                     /locus_tag="ACHBFW_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFM9316723.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            31635..32024
                     /gene="rpsK"
                     /locus_tag="ACHBFW_000376"
     CDS             31635..32024
                     /gene="rpsK"
                     /locus_tag="ACHBFW_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFM9316724.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            32104..33042
                     /locus_tag="ACHBFW_000377"
     CDS             32104..33042
                     /locus_tag="ACHBFW_000377"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFM9316725.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            33070..33450
                     /gene="rplQ"
                     /locus_tag="ACHBFW_000378"
     CDS             33070..33450
                     /gene="rplQ"
                     /locus_tag="ACHBFW_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFM9316726.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            33722..34561
                     /locus_tag="ACHBFW_000379"
     CDS             33722..34561
                     /locus_tag="ACHBFW_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9316727.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            34537..35406
                     /locus_tag="ACHBFW_000380"
     CDS             34537..35406
                     /locus_tag="ACHBFW_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9316728.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            35396..36193
                     /locus_tag="ACHBFW_000381"
     CDS             35396..36193
                     /locus_tag="ACHBFW_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM9316729.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            36396..37142
                     /gene="truA"
                     /locus_tag="ACHBFW_000382"
     CDS             36396..37142
                     /gene="truA"
                     /locus_tag="ACHBFW_000382"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM9316730.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            37806..38000
                     /locus_tag="ACHBFW_000383"
                     /pseudo
     CDS             37806..38000
                     /locus_tag="ACHBFW_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT        12298 a         6961 c         8399 g        10531 t
ORIGIN      
        1 aataagatac agaacttgta tagaaggctg ccaagtcggt agataaagga ggtttttatc
       61 aaaaaacata ctttcctttt ttctctattc ttttcttggg ggttttggta cactgatagg
      121 gaatttagat agaagggagc caataggatg aagctgacca tcagagatat cgcagaaatg
      181 gcaggcgtat cagtaacaac tgtctcacag attttgaata acaaaggaag cagatttagt
      241 gataagacaa gaaaaaaagt gttgtcgatc gtgaatgaac atcattataa gcccgattat
      301 ttcgcttcaa atgtgatcaa tcgacattca aagaccatcg gaatgatcgt ccctgatgtc
      361 actgatttct tcttctccaa actgatcgaa ggtgtagaga gctatttgaa tcctttgggc
      421 tatgtgatca tgttatgcaa ttctcgtcat agccaagaaa atgagatcaa gtatttacaa
      481 gagctttccc accgctcagt ggacggaatc ttgctggcca caccaaatat tttacctgag
      541 aaatatgcat tagacagcgg tttttatcaa aaaatgccaa tcattttaat cgatcgcgga
      601 ttgaatcgtc gggataacgg acggcttatc gtaaaggaat atgagggcgc ctatcaagct
      661 gtttcgctgt ttattgaaaa tggccatacg aagatcggca tgctgaagga aaccactgga
      721 tactatcaat tagaagaacg tttcaacgga tatcgtcatg cgctgaaaga tcatggcctg
      781 ccttttaatg ggaaatttgt tgaacatgga gagttgactg tccaaggtgg atatactgca
      841 tcaaaaaaat tactcaaaca taaagatatc acggctattt tttgcgggaa tgatgccatg
      901 gcgatcggat gctatcaggc gatcgatgaa ttgggcaaaa aaatcccaga agatatctca
      961 gtcataggat tcgatggatt aaagctgtca gaatacatga ttccgcaatt gacgacagtg
     1021 cagcagccaa gttttgatat tggattttat gctgcacgtt tcctgatcga tacgatcgaa
     1081 tttcctcaaa gaaaagttcc gaacaaagtg ttcgaaacaa aattaattat tcgtgagagc
     1141 gtaaaagtat tgacaaaggg ctaactcggc ggtatattgc ttatgtaagc atttacaaag
     1201 gctagttagt gcttttttta tttttagtaa accagtttac taaagtggtt tacctttcct
     1261 aaaaataatg tataataccg aatgaagaca aggaggatca ggcaatgcga atggtggatc
     1321 tgattgaaaa aaagagggat ggaaacgagc tgtcgaaaga ggaaatcgag tacatcgtca
     1381 cgaactatac gaatggaaag attccagatt atcaagtcag tgcattattg atggcaattt
     1441 tttatcaaga catgacaaat gaagaaatca cgaacttaac attggcgatt gctaattctg
     1501 gtgatgtcat cgatttgagt tctcttgaag gaataaaggt agataagcat tcgacaggtg
     1561 gcgttggtga cacgaccaca ttgattttag cccccctagt agctagtgtc ggagtgacag
     1621 tggctaaaat gtctggacgc ggtctaggtt acacaggtgg tacactagac aagctagaag
     1681 caattcccgg atttcagatc gagctttcgg atgaagaatt tgtacgcatt gttaatgaaa
     1741 gtaaagtagc ggtaattggt caatcaggaa acttggcacc ggcggacaaa aaattgtatg
     1801 ccttgcgtga cgtcacagcg accgttgatt ctcttccgct gattgccagt tcgattatga
     1861 gtaaaaaaat cgcggcagga gcagatgcaa tcgttttgga tgtgaccact ggagacggtg
     1921 ctttcatgaa gaacatcgaa gatgcaagac gtctggcaaa gaccatgacg agcattggga
     1981 aactggctaa ccgagaaaca gtggcggtta tctcagatat gtcagaacct cttggcgaag
     2041 ctatcggaaa tagtctagaa gttgtcgagg caatcgaaac attacaagga aatggtccag
     2101 aagatctagt agaaatgtgc tacgccttag gaagccaaat ggttgttctt gctggcaaag
     2161 ctaagacaac cgatgaagca agaacgttgc ttcaagaagc tctggaatca ggtaaagcgt
     2221 tagcaaaatt caaagaaatg atccaaaatc aagggggaga cccagcgatc gtggaacatc
     2281 cagaacgaat tttgactgca cgctacacaa tggagcttcc tgctaaacaa agcggagttg
     2341 tgtcaaaaat cgtggcaaat gaattaggaa ttgctgcaat gatgctaggt gctgggcgta
     2401 aaaccaaaga agaagatatt gatcacgctg ttggattgaa actgcataaa aaaattggtg
     2461 acactgtgac aaaaggtgag tcactattga cgatctatag taacgacgaa gaaatcactt
     2521 ctgtcattga attattatat aaaaatattg aaattggtga atcggcaatg aagccaacgt
     2581 tgattcatga catcatcaca gaataaagga aaaaagcgga ctttaccgtt ttaaagaaaa
     2641 ggggaaaaga aatggaacta aaccgaatga tcgaccatac tattttgaaa gcagacgcat
     2701 ctaaagaagc tgtgatgcaa attatcgaag aagcaaaaaa ataccatttt tattctgtct
     2761 gcatcaatcc agcatgggtt gcgctagcga aagaacaatt acaaggtgag ccagtagccg
     2821 tatgtacagt aatcgggttt cctctaggag caaacacacc cgagacaaaa gcatttgaaa
     2881 caacagatgc catcaataac ggtgcagatg aagtcgatat ggtcatcaat atcggtgctt
     2941 tgaaatcaaa ggatgatcaa caagtccaaa aagatatcga agcagttgtt gaagcagcaa
     3001 aagatcgtgc gcttgtcaaa gttattattg aaacatcgtt attagataga gaagaaatca
     3061 tccgagcttg cgaaatagca aaagctgcag gcgctgattt tgtcaaaaca tcaactggtt
     3121 tctcaacagg cggagcaaag gtagaggatg tccgtttgat gcgtgaaact gtaggaccag
     3181 aaatgggtgt caaagcttcc ggcggtatcc ataacgaaga agaagcaatg gcaatgatcg
     3241 aagctggtgc tacaagaatc ggaacaagcg caggagtagc aatcgtatct ggttcaactg
     3301 gtgaaggcta ttaatttttt caaaggagaa gctcatggca gaactcaaac aagaatggat
     3361 caacacagct attgaagcgt tagataaagc ctatgttcct tattcccatt ttccggtagg
     3421 cgcttgtctc gtaacggaat caggcaagat ttatcaaggc atcaacattg agaatgcatc
     3481 atttggatta acgaattgtg ccgagcgcac agcttttttc aaagcagtat ctgaaggaga
     3541 aaaaacattt acgcatctag tcgtagcggg gcacaccccc gatcctatct ctccttgtgg
     3601 tgcatgtcga caagtaatgg cagaattctg ttcccctgat atgccggtta cgctagtagg
     3661 agacaatggt gtgacgaagg caacgaccgt tcgtgcactg ctgccttatg catttactga
     3721 aaaagatcta taactcatgt tcattcagtc agctgactgt tgaaaaataa aacaattatt
     3781 ttaggaggct ttacagaaat gaaaaaagca aaattatttg gtttaggtgc tgtcgcacta
     3841 gcagcagggt tatttttagg tgcatgcggc aacaacggct ctactgattc atccggtggc
     3901 aaaagctcat cagacacaac aactgcagca ttgatcacag acacaggtgg tgtagacgac
     3961 cgttcattca accagtcagc ttgggaagga ctagaaaaat gggggaaaga tcaaggactt
     4021 tcacgaggaa atgatggctt ccaatatttc caatcttcaa atgaatcaga ttacatccca
     4081 aatattgacc aagcattgaa tgctggattc aaaacgattt ttggtattgg ttataaacta
     4141 aaaccagcga tccaagaaca agcaacaaac aatacaggta cgaactttgt tattatcgat
     4201 gatgtcatcg atggattaga taacgttgta tctgcaactt tcaaagacaa cgaagcttct
     4261 tatcttgcag gtgttgcagc agcttatacg actgaaacaa acgtagtcgg ctttatcggc
     4321 ggtgtaaaag gtgaagtaat cgatcgtttt gatgctggat tcaaagccgg tgttgatgca
     4381 ggtgcgaaag aattaggaaa agaaatcaaa gtattgaatc aatatgctgg tgacttctct
     4441 gctccagata aaggacgttc aattgctcaa ggtatgtatg cacaaaacgc agatatcatc
     4501 ttccatgctt caggcggtac aggtaacggt gtattccaag aagcaaaatc attgaacgaa
     4561 tcaggcgaca aaaaagtttg ggttatcggt gttgaccgcg accaatcaga cgaaggcgaa
     4621 tacacattaa acggcgaaaa gaaaaacttc acattgacat ctacactgaa agcagttgga
     4681 acagtagtag aagatttggc acaaaaatca gcggacggta aattccctgg cggcgaacac
     4741 actgtttatg gattgaaaga agacggtgtt ggtctaacag aaggacaact ttctgacgaa
     4801 gcgaaaaaag cagttgatga agcaaaagaa aaaatcattt caggtgacgt aaaagttcca
     4861 gaaactcctg aagaaaacta aaaccatttc tatagccttg ttagacacat gtttgacaag
     4921 gctatggttt gattagaaca agaattgttc atttatccaa gctgccttgt gacagtttgg
     4981 ataagggaac aactccctga agtaatgttt gtttcaaaag aagaacgctc actaagcgtg
     5041 aatcttagtc aatcgttgcc ctagatagaa atcgttagga tgtgaaaaaa aggtggttca
     5101 agaaaatatt gtcatagaaa tgcagaatat taccaaagaa tttggaacat tcaaggcaaa
     5161 tgacaacatc aatttacaag taaaggcagg agaaattcat gcgttgcttg gagaaaacgg
     5221 tgctggcaaa tctacattga tgaacgtgct ttccggatta ttagagccga catcagggaa
     5281 aattttgatg cgtgggaaag aagtaaagat cacaagtccg accaaagcca accaattagg
     5341 gattgggatg gtccatcagc actttatgct tgttgatgcc tttactgtaa cagaaaacat
     5401 cgtgttggga agcgagccta gtcgcgcagg gatgcttgac cataaaaaag cacgaaaaga
     5461 gatccaaaaa gtttctgaac agtatggatt gtcagtcaac ccagatgctt acgttcgtga
     5521 tatttcagtt ggaatggaac aacgggtaga aattttaaaa acactttacc gaggagcaga
     5581 tgtactgatt tttgatgagc cgacagctgt attgacccct caagaaattg atgaattaat
     5641 cgtgatcatg aaggaattag tcaaagaagg caagtcgatc attttgatta cgcataagtt
     5701 agatgaaatc aaagcagtag ctgaccgctg tacagttatc cgccgtggaa aaggaatcgg
     5761 tacagtcaac gttaaagacg taacctcaca acaattagcc gatatgatgg ttggaagaac
     5821 ggtttcattc aaaacgatga aaaaagaagc gaagcctcaa gaagtcgttt tgtctattga
     5881 aaatctagtg gtgaaagaaa atcgtggatt agaagccgtg aaaaacttga acttagaggt
     5941 tcgtgctggc gaagtacttg gtatcgctgg aatcgatgga aacgggcagt cggagttgat
     6001 ccaagctttg actggtttgc gaaaggcaga aagcggacat atcaagctaa aaggggaaga
     6061 catcactaat aaaaaacccc gaaaaatcac tgaacatggt gtaggacacg tgccagaaga
     6121 ccgtcataaa tacgggttag tcctagatat gacattatct gaaaatatcg cactacaaac
     6181 ctatcatcaa aaaccttaca gtaaaaacgg catgctgaat tattcagtaa tgaatgaaca
     6241 tgccagagaa ttgatcgaag aatatgatgt tcgaacaacg aatgaacttg ttcccgcaaa
     6301 agctttatca ggcggaaatc agcaaaaagc aattatcgct cgtgaagtcg accgagatcc
     6361 tgatctgttg attgttgcaa atccaactcg tggactggat gtaggtgcga tcgaatttat
     6421 tcataaacgt ctgatcgaac aaagggacaa atacaaagca gtgttactga tcagtttcga
     6481 attagaagaa attttaaatg tttcggatcg tattgctgtt atccatgaag gagaaattgt
     6541 cggtatcgtt gatccgaaag aaacatctga aaatgaatta ggtctattga tggcgggcta
     6601 taccttagaa gaagcacgaa aagagttaga aaagggggct ggcgcacatg aatgatcgca
     6661 gcgaaaaatt ccgtaattta ttagtgcctg tactttcagt tgttttagga ttgatcttag
     6721 gcgcgatatt gatggctgct tttggttttc agcccatcca aggttattct tctatgctag
     6781 atgcagccct tggttcgcaa agagggatag gtgaaacgtt aaggcaagca acgccgttga
     6841 tttttacagc attaggtttt tctgtagcta actcagctgg gtttttcaat attgggcttt
     6901 ctggacaagc actttgcggg tggatcgtca gtgtctgggt ggcacttgcg tttccagatc
     6961 tgccaaaagt tgttttgctt ccgttatgcg tgatcttagg tgccttagct ggtgcgatgg
     7021 cagcggctgt tccaggttta cttcgagcat tttttggaac aagtgaagtg atcgtaacaa
     7081 tcatgatgaa ctatatctta ttatacgtaa gcacatttgc tttacatgaa gtgatgcctg
     7141 aaagctatcg ctcaagtttg gactcttcca atatgatcag tcaaaatgcc tcactgcggt
     7201 taccatggtt aacacaagct tttggcggct ctcgcgtaaa ctcaggatta tttttagcaa
     7261 tcatcgcgct tattttcgtt tggctgctga tgaagaaaac gactttagga tttgaaatcc
     7321 gttctgttgg attgaatcct tttgcttctg agtatgcagg aatgagtagt aaacgtacga
     7381 tcgttttgtc gatggtcatt tctggcgctt tggcagggct tggcggtgtg gttgaaggat
     7441 taggaacgta tcaaaacttc tttgttcaaa cgacttcgct atctattgga tttgacggaa
     7501 tggctgtttc cttgcttggt tctggatcag caatcggtat tcttttatca gcgatcttgt
     7561 tcagtatttt gaagatcggt ggacttggca tgcagacagg agcaggtgtt ccgtttgaga
     7621 tcgtcaatgt atcgattgct ttgattatct tctttgtcgg gatcaacttc ctgattcgtt
     7681 tcgtcatggc taaagtcttc caagggaaaa aacaagaaga gatcgttgcg acaatagaat
     7741 cgaaaccaac gaatcaaaac catgaagggg gcgaactata atgtcgacta tcgaattaat
     7801 tgccttgatc cttacttcta cactggttta ttcaacaccg ctaattctaa cgtcactagg
     7861 cggaactttt tctgaacgaa gcgggatcgt aaacgtaggg ctagagggaa tcatggtcat
     7921 gggtgctttt agttccatcg ttttcaactt gacgatgagt ccatcacttg gttctatgac
     7981 accatggatc gctattttag tgggtggcgt cgtaggtatc ctattttctc tgattcacgc
     8041 agtagcaaca gtcagtttgc gtgctgacca tattatcagc gggacagtaa ttaatctgat
     8101 ggcacctgct ttaggtgttt tcttgatcaa agcatggtat ggtaaaggtc aaactgataa
     8161 catcacgcag aatctggggt atttttcttt cccaggatta gcagatattc cggtcattgg
     8221 accaattttc tttaaaaata cattcttacc tgcttattgt gctattttga ttgctgtcct
     8281 agcttgggtg atcatcttca agacgaaatt cggcttgcgc ctccgttcag ttggtgaaaa
     8341 tcctcaagct gccgacacat taggtatcaa tgtatatgct atgcgttatt caggcgtact
     8401 gatttctggc ttgttaggcg gtatgggcgg tgctgttttt gctcagtcga tttccggtaa
     8461 cttttcagct ggtacgatcg ttggacaagg gttcatctcc atggctgcta tgatctttgg
     8521 taagtggaat cctttagggg caatgggtgc ctcattgttc tttggttttg ctcaaagctt
     8581 gagtatcatt ggcgcacaat taccagttat ttcttcgatt ccgccagtat tgctgcaaat
     8641 tgctccatat ttgctgacaa tcattgtgct ggttgttttc ttagggaaag cttctggtcc
     8701 aaaagcaaac gggaagaact atatcaaatc aaaataatct tttagcgaat aaaaagggtg
     8761 ggttacggga agtgtttttc gtctcgccct ttttgtttta aaggcaataa aaataaaaag
     8821 aaagaaggaa taatcatgtt taaacgcgta catctgatcg tcatggattc agtaggaatt
     8881 ggagaagcac cagatgccga taagttcggt gatgttggtt ctgatacatt aggacacatt
     8941 gcgaaagaag caggattgaa cgttccacat ttagagcagc ttggtttagg aacgatccgt
     9001 cctcttgaag gtgtggaatc agtgaaagat catcaaggct atgcgacgaa attagaagaa
     9061 gtttctgtag ggaaagatac gatgacaggc cactgggaaa tcatgggatt gaatatcaaa
     9121 actccatttc gtgtgtttcc taatggattc cctgatgatc tgatcaaaca aatcgaagaa
     9181 ttttctggcc gtaaagtcgt atgtaacaaa ccttatagcg gaacagctgt tattgacgat
     9241 tacggtgaac accaaatgaa aacaggcgat ttaattgtat atacttctgc cgatcctgtc
     9301 cttcagatcg cggcacatga agaaatcatt cctttagaag aattatatcg gatttgccaa
     9361 ttcgttcgtg atataacaaa agatgatccg tatatgattg gccgaatcat tgcacgtccg
     9421 tacgtgggtg aaccaggaga cttcacacga acaagcaatc gccatgatta tgcgttgaat
     9481 ccattcggac acacagtcct tgattctttg aaagaagcag gaaaggacgt gattgctgtt
     9541 ggaaagatca acgatatttt caacggacaa ggaatcactg aatctgtccg cacgaaaagc
     9601 aatatggatg gcgttgacca gctgctgaaa gttatgaaaa acgatttttc aggaatcagc
     9661 tttaccaatt tagtagattt tgatgcatta tacggtcatc gtcgggatgt agtcggctat
     9721 gcgcatgcga ttgaagaatt tgacttacga attccagaat tgcttgaagc gatggaagaa
     9781 gacgacttgc taatgattac agccgatcac ggaaacgatc cgacgtttac tggtaccgat
     9841 catacgcgag aatatgtacc gttattggtt tacagtaaaa aaatgaaaga acaaggggca
     9901 ttgcctcaag gatattattc agatatttca gcaacgattg ctgagaactt tagtgtttct
     9961 tcgactgaaa atggcgaaag cttcttgaaa ttattaaaat aatcttttta aataggagaa
    10021 agcaaatgac caagttaagc gagatgttac aagaaacgac ccagtttatc cttgataaag
    10081 gagtaaaaga aattgatttt ggtttgattc taggttcagg attaggtgaa ttagcagatg
    10141 aaatcgaaga tgctgtaatg atttcttatg atgagatccc gttctttcct gtttcgacag
    10201 tagttgggca tgcaggaaag ctagtttatg ggtctctggc aggcaagaaa gtgttagcca
    10261 tgcaagggag attccactac tatgaaggtc attccatgca aacagtgacc tatccagtac
    10321 gtgtgatggc cggactaggg gcccattcag tcattgtgac taatgcatgt ggtggtgtca
    10381 acgaatcatt tgtaccagga gatttgatgc tgattaccga tcatatcaat tttactggtg
    10441 aaaatccttt gatcggtccg aatgaagatg caatgggccc tagatttcca gatatgagtc
    10501 aagcttatac gcctgactat caagcaacag caaaagcagc agcagaaaaa ctaggtttgg
    10561 atcttaaaga aggcgtatat atgggctatt ctggaccaac ttatgaaacg cctgcggaga
    10621 tccgaatgtc tcggacaata ggagccgatg cagtagggat gtcgactgtt ccagaagtca
    10681 tcgtagcagc tcacagcgga ttaaaagtgt taggaatctc ttgtattacc aacttagcag
    10741 caggtatgca agcgaatctg agccatgaag aagttgtaga aacaactcag agagtcaaac
    10801 aatcttttaa agcattggtt aaagaaacat tgacattatt gtaataggag gaacaataaa
    10861 atgagtgtgc atattgaagc gaaagaaggc gagatcgccg ataagatttt acttccggga
    10921 gatcctttgc gagctaaata tattgcggag acttttttgg aagatccagt ttgttacaat
    10981 caggtacgcg gtatgttagg ctttaccggc aaatataaag gagaacgtat ttctgttcaa
    11041 ggaacaggaa tgggaatgcc atcagccacc atctatgccc atgaattgat ccagtcgtat
    11101 ggtgtaaaga aattgattcg agtaggtaca tgcggtgcat tgtcaaaaga cgtccatgtt
    11161 cgtgatttag tactagcaca aggtgcagcg accagttctt cgatgatcga aaaaaatttc
    11221 caagcctttc attttcctcc aatcagtgat ttcaatctat tactgaaagc atatgagata
    11281 gctaaagaaa agggctatac ggttcatgta ggaaatgttc tttcggaaga ttctttctac
    11341 aaagatgatt tgacagagac gttccaatta gctgaacttg gcgttttagg agtggaaatg
    11401 gaagcagcgg ctctttatta cttaggcgcg aaataccatg tgcagacatt aagtttgatg
    11461 accgttagcg accatttgat caccggtgaa gaaacaacag ctgcagaacg tcaatctacg
    11521 ttcaatgaaa tgatcgaagt aggattggaa acagcgatag cagaataaga aggaaatgaa
    11581 aaagctgccc tgcattaagt aataagaaca tccaagcaaa aatagctcct catgtttttg
    11641 acttggctgg acttattatt gcaaatgtaa gcttttgaac accgtttacc aagaggttgt
    11701 gaatctgccg tttaactctg agtcacaagg aacaatttca aaaaacagct tttcatgttt
    11761 gatgaatatt gtgacttgtg accgagaagt tggcaggaga aaaccgttta tacgaaaaaa
    11821 gggactgtga caaatcgtgt cacagtccct tttttatagt gttttttata ggtagtattt
    11881 tttagcaact gttaagtcat catttaattc ataaacaagt ggttgacctg ttggaatttc
    11941 aagatccatg atgtcttcgt cagagatacc ttcgatatgt ttagctaggg cgcgtaatga
    12001 gttaccatgt gcagctacta aaacagtttt gttgtctagt aatgctggag caatttcatc
    12061 ttgccagaat ggcaaagcgc gttctaatgt cactttcaag ttttctccac caggaatatc
    12121 gcgtttgtct aacattgcgt aacgacggtc atttgctgca gaaccttcgt cagtttcttc
    12181 cattaatgga ggaagagtat cgtaagaacg acgccagatg tgcacttgtt cgtcgccgta
    12241 tttttcagct gtttcttttt tgttcaaacc ttgtaattta ccatagtgac gttcgtttaa
    12301 gcgccatgat ttgatttgtg gtacccaaag ttgatctgaa tattctaaga tcaagttaca
    12361 tgttttgatt gcacgagtta aaacagaagt gtaagcaaca tcaaattcga ttccagcttc
    12421 tttgattttg cgtccgcctt cttttgcttc ttcgattcct tctggtgcta gatttacatc
    12481 agcccaacca gtaaattggt tcaatgcatt ccattcacta agaccgtgac gagaaaaaac
    12541 taattttggc atgtttagct ctccttttaa gataggttta tatttctgaa attttcagtt
    12601 ctgttttatt gtacaatttt ttggaagaga tttccatcga aaaattagat aagattctat
    12661 gagaaaacaa gtagagaata taaaaaaacg aacagtgccc acgcaactgt tcgtttttag
    12721 gtattagatg cataattgct cgatttcttt ttcaggtaaa ttgatatatt tataacatgt
    12781 tccaactgaa acaccgcatt ttgaagaaat aaattggatc gtttcttttt tttcataata
    12841 gagacgtcgg atttttttga tcgtttttgc atcgattttt ggtcttccgc cgatttttcc
    12901 ttttttacga gcttcatcta atccaactaa tgtacgttct ttgatcaatg cgcactccat
    12961 gtttgctagg ttttccatta attggaaata gatttttccc atttcgcgcg tatcgattcc
    13021 ttcatcaaga ctaatcaagt gcagcccgcg atctttgaat aattgcgtga gttctgttaa
    13081 ttgccgcgta gatttgccta atcgatgaag ttggcaaatg actagtgcat cgccttgttg
    13141 catgtcagcg atcactgact ccaaatgaac agcagtagaa tcattttgtt ccacttcata
    13201 tgtctcatgg aaaatttctt gacaaccaaa gtcagataat tgatcaacct gagttgttaa
    13261 atcaacgtca tttatggttg tgcgtgcata accaataatt ttcatggtga ttcctttcta
    13321 gcagtgattt tctaactgga tataacagta aaaatgtagc ataaagtacg tcaaaaagat
    13381 agttttttcg caaattttcc gctgtctttt tttattggat gatcaagcag tttttcttta
    13441 tttacgacgg attgcgtcaa gaatctcaac gcctttttct gagccgacaa ctactttaga
    13501 aaccatgttt aggaaaagcc catgttctac taccccaaca agttcatcta aataggtagc
    13561 taagctttct ggctgttcga tgacttctag atgcagatca atgatgtaat gaccaccatc
    13621 tgtggttaag acttctccgt tttcgtctgt acgcaagacg ggttggaatc ttttttcttc
    13681 aaaaatatga agtagctgtt gggagccata agggatcact tcaactggga gtgggaactt
    13741 cccaagttta tcgactaatt tagaagaatc aacgatccaa atcgtttctt ttgagtacgt
    13801 agcgactatt ttctcgaaaa gcaaagcagc gccgccgcct ttgatccctt gaaaatcagc
    13861 actgatttca tcagcaccgt ctattgtcaa atcgactacg gggacttcat caatactctt
    13921 taacggtatt cctaattttt ccgcttgttc ttttgtcgca tttgaagtag taactcctgt
    13981 gatacgtaaa ccttcttcac gcatacggcg accaatctct tctaccatat aataggcggt
    14041 tgaaccggtc ccgagaccta cgatcatccc gtcctttacg aatttcgcgg cttcaatgcc
    14101 agctaattgt tttaaattca tctttgcgtc cacctttttt tagtttgagc atcgtgcttt
    14161 cctcactatt ttagcgtgat ttacggtaaa aagataccat tcttttttat tccaataaag
    14221 ctttttcaaa aaaaattgca tctcttgttg acatgttttt tcattcgtga tattctaaca
    14281 aaggtgcttt atctacaggt ttctgtgaag aaacgtgctg actgtttatc aaagcatatt
    14341 tgataaggat gcagacattg catttatttt ttatcacaaa aaaagaacca cctggatgtg
    14401 tggaactaga cgcaaatcgt gggaaggagg aaacaaggaa tgcctacaat taaccaatta
    14461 gtacgtaaac ctcgtaaatc aaaggtggaa aaatctaatt cacctgcttt gaacaaagga
    14521 tataacagct ttaagaaaac tcaaactaac gtaaactcac ctcaaaaacg tggggtagca
    14581 actcgtgtgg gtacaatgac acctaaaaaa ccgaactcag ctttacgtaa atatgcacgt
    14641 gttcgtttgt caaacttaat tgaagttact gcttatatcc cagggattgg tcataactta
    14701 caagaacaca gtgttgtatt attacgcggt ggtcgtgtaa aagacttacc aggggtacgt
    14761 taccatatcg ttcgcggtgc tttagatact gctggtgtta acgaccgtaa acaaagccgt
    14821 tctaaatacg gaactaaaaa acctaaagct taattttaat tattaaacaa atgaaacttg
    14881 cttctactat atagaagtaa ttactatgaa caatatttcg gaaggaggag ttacggatgc
    14941 cacgtaaagg tcctgttgca aaacgtgatg ttttaccaga tcctatttat aactcaaaat
    15001 tagtaactcg cttgatcaac cgtgtaatgg ttgacggaaa acgcggtatt gcagctaaca
    15061 ttatctataa tgcctttgac ttgatcaaag aatctacagg taacgatcca ttagaagtgt
    15121 ttgaacaagc aatgaaaaac gttcaacctg ttcttgaagt taaagcacgt cgtgttgggg
    15181 gttcaaacta ccaagtacca gttgaagttc gccctgaacg ccgcgttact ctaggtcttc
    15241 gttgggtagt aaactacgct cgcttgcgcg gtgaacacac aatggaagaa cgcttagcaa
    15301 aagaaatcat ggatgctgca aacaacacag gtgcttctgt taaaaaacgt gaagacactc
    15361 ataaaatggc tgatgccaac cgtgcgtttg ctcactatcg ctggtaagat tctctctgtt
    15421 tggtcggctt ttataagtcg acctcagata gtgtccttat ttttagcaac tagatagtaa
    15481 agagaggaga aacaaggaga atgaccagag aattttcttt acaaaacact cgtaatatcg
    15541 gtatcatggc ccacatcgat gctggtaaaa cgactgctac tgaacgtatc ttgtactata
    15601 ctggtaaaat ccataaaatt ggtgaaactc atgaaggagc ttcacaaatg gactggatgg
    15661 aacaagaaca agaacgtggt attacgatca cttctgctgc tactacagca caatggaagg
    15721 gtcaccgaat caacatcatc gacacaccag gacacgtaga cttcacagtt gaagttgaac
    15781 gttcattgcg tgtacttgat ggtgctgtaa ctgttcttga tgcacaatca ggtgttgaac
    15841 ctcaaactga aacagtttgg cgtcaagcaa caacttatgg tgtacctcgt atcgtatttg
    15901 caaacaaaat ggacaagatc ggtgctgact tcttatattc agtatcaact ttacatgacc
    15961 gtttgcaagc aaacgctcac ccaatccaat taccaattgg tgcggaagac gacttcacag
    16021 gaattatcga ccttgtaaca atgaaggctg aaatgtatac aaacgatctt ggaacagaga
    16081 tcgaagaaac agaaattcct gaagaatacc gcgaattagc tgaagaatgg cgcgaaaaat
    16141 tggttgaagc tgtagctgaa actgatgaag aacttacttt gaaatactta gaaggtgaag
    16201 aaatcactga agctgagtta aaagaaggta tccgccgcgc aacagttaac gtagaattct
    16261 acccagtact atgtggttca gcatttaaga acaaaggggt tcaattacta ttggacgctg
    16321 ttcttgatta cttgccatca ccgttagata tcccagctat caaagggatt gaccctaaaa
    16381 ctgatgaaga agtagaacgt cctgcagacg acagcgctcc tttctcagct ttagcattta
    16441 aagttatgac tgacccattc gtaggtcgtt taactttctt ccgtgtgtac tctggtgttt
    16501 tacaatcagg ttcatacgta caaaacgcaa caaaaggcaa acgtgaacgt gttggtcgta
    16561 tcctacagat gcatgccaac tctcgttcag aaatctctga agtttacgct ggagatatcg
    16621 ctgctgctgt tggtttgaaa gacacaacta ctggggacac actatgtgat gaaaagagtc
    16681 tagttattct tgaatccatg gaattcccag aaccagttat ccaagtcgct attgaaccta
    16741 aatcaaaagc tgaccaagat aaaatgggtg tggctttaca aaaactatct gaagaagatc
    16801 ctactttccg tgctgaaaca aacgtggaaa caggcgaaac aatcatcgct ggtatgggtg
    16861 aacttcactt agatatcatc gttgaccgta tgcgtcgtga attcaaagtt gaagctaacg
    16921 taggtgcgcc tcaagtatct taccgtgaaa ctttccgtgc tggcactcaa gccgaaggta
    16981 agtttgtacg tcagtctggt ggtaaaggtc aatacggtca cgtatggatt gaatttacac
    17041 caaatgaaga aggtgctggt ttcgaatttg aaaatgcgat cgtcggtggg gttgttcctc
    17101 gtgaatacat tccagctgtt gaaactggtt tgaaagatgc tatggaaaat ggtgtcttag
    17161 ctggctatcc attagtagat atcaaagcta aactttacga tggttcatac catgatgtcg
    17221 attcaaatga aactgccttc cgtgttgctg cttctatggc attacgtgca gcagctaaga
    17281 aagctaatcc agtaatcctt gaaccaatca tggctgtaga agttgttatc cctgaagatt
    17341 atttaggtga tgttatgggt cacgtaactg ctcgtcgtgg acgtgttgaa ggtatggaag
    17401 ctcgcgctgg cggccaacaa gttgttcgtg cgatggttcc acttgctgaa atgtttggtt
    17461 atgctacaac tcttcgttct gctactcaag gtcgtggtac atttacaatg acatttgacc
    17521 actacgaaga tgtaccaaaa tcagtacaag aagaaatcat taagaaaaac ggcggaaaag
    17581 ctgaataatt tcttgaaacc ggatagtcct gcaaagagat tatccaaaga gactatattt
    17641 ttaatatagt ttcgtgtaaa ataattatcg atgatatggg catgtggtgt accttgtgta
    17701 catcatatac ctatcagaca aataaaaaat atattgtatt tttaggagga cattttaaaa
    17761 tggcaaaaga aaaatttgac cgttctaaac cacacgttaa cattggtact atcggacacg
    17821 ttgaccatgg taaaactaca ttaacagctg caatcacaac tgtactatct aagaaaaacg
    17881 gcggccaagc tatggcttac gatcaaatcg atggtgctcc agaagaacgc gaacgcggaa
    17941 tcacaatctc aacagctcac gttgagtatg aaacagatac acgccactac gctcacgttg
    18001 actgcccagg acacgcggat tacgttaaaa acatgatcac tggtgctgct caaatggacg
    18061 gagctatctt ggtagtttct gctgctgacg gcccaatgcc tcaaactcgt gaacacatcc
    18121 tattgtctcg tcaagttggt gttccttaca tcgttgtatt cttgaacaaa gtagacatgg
    18181 ttgatgacga agaattacta gaattagttg aaatggaagt tcgtgaccta ttaacagaat
    18241 acgaattccc tggtgacgat gttcctgtag ttgctggatc agctttgaaa gctctagaag
    18301 gcgacgcttc atacgaagaa aaaattcttg aattaatggc tgcagttgac gaatacatcc
    18361 caactccaga acgtgacaac gacaaaccat tcatgatgcc agttgaagac gtgttctcaa
    18421 ttactggacg tggtactgtt gctacaggtc gtgttgaacg tggacaagtt cgcgttggtg
    18481 acgaagttga agttgttggt attgctgaag aaacttcaaa aacaacagtt actggtgttg
    18541 aaatgttccg taaattgtta gactacgctg aagctggaga caacattggt gctttactac
    18601 gtggtgttgc acgtgaagac atccaacgtg gacaagtttt agctaaacca ggtacaatca
    18661 cacctcatac aaaattctct gcagaagtat acgtgttgac aaaagaagaa ggtggacgtc
    18721 atactccatt cttcactaac taccgtccac aattctactt ccgtacaact gacgtaacag
    18781 gtgttgttga attaccagaa ggaactgaaa tggttatgcc tggcgacaac gtaacaatgg
    18841 aagttgaatt aatccaccca atcgctatcg aaaacggtac taaattctca atccgtgaag
    18901 gcggacgtac agttggtgcc ggtgtcgtta cagaaatcaa agcttaattc acttaagttt
    18961 taaagtaaag caacatttat tcggacgtaa tcgctcggaa cgaaggtctt aggatctttg
    19021 ttccgagctt ttttatttat tgcaggatca ggaaaaaaga caggagtatt ataaatagaa
    19081 gaaacacgaa ataaaagagt cagtattttc taaatgaaac tttatgaaaa caaaatgttt
    19141 tttacaagaa aaagccttgc tttgtgagtt tatatatatt ataatgacaa aggtgcgttt
    19201 actttagtga attgcacaga cgtaatcaga atgatttcgt cggctaagaa acgagaggtt
    19261 gcgacacgcc cggacgcttt gccacgaacg agcgtgacgg aaattttcgt ggagctatgt
    19321 ctacttttca aaataggcga aggagggaac aagatggcaa aacaaaaaat tcgtatccgt
    19381 ttaaaagcgt atgaacaccg tattttagat caatcagcgg ataaaatcgt ggaaactgca
    19441 aaaagaactg gagctagcgt gtctggtcca attccattac caactgaacg cagtctttac
    19501 acaatcatcc gtgcgactca taaatacaaa gattcacgcg aacaattcga aatgcgtaca
    19561 cacaaacgtc ttatcgacat tgtgaaccca acaccaaaaa ctgttgatgc tttgatgaag
    19621 ctagacttac catctggtgt aaacatcgaa atcaaattat aaaactaaaa caaaaataat
    19681 ggaggtgtac tcatgaccaa aggaatctta gggaaaaaag tgggaatgac acaaatcttc
    19741 actgaatctg gtgaattaat tccagtaact gttgtagaag ctactccaaa cgtagtacta
    19801 caagtgaaaa caatggaaac ggatggctac gaagctattc aagttggtta ccaagatatg
    19861 cgtgaagttt tatcaaacaa acctgcgaaa ggtcatgttg caaaagcaaa cacggctcct
    19921 aagcgcttca ttcgtgaatt caaaaatgtt gagctaggag aatatgaagt aggaaaagaa
    19981 attacagtag atgttttcca agcaggagac attattgatg taacaggtac tacgaaaggt
    20041 aaaggattcc aaggggttat caaacgccac ggacaaagcc gcggaccaat ggctcacggt
    20101 tctcgttacc atcgtcgtcc tgggtcaatg ggtccagttg cacctaaccg tgtatttaaa
    20161 aacaaacgtc ttgctggccg tatgggtggc aaccgcgtaa ctatccaaaa cttggaagtt
    20221 gtacgtgtcg atgcagaaag aaatgttatc ttgatcaaag gtaatgttcc tggagctaaa
    20281 aaatctttaa ttactatcaa atcagctgtg aaagctaaat aattgtagga agaaaggagg
    20341 aactaaggaa tgccgaatgt agcattattc aaacaagatg gaagccaaaa cggtgaaatc
    20401 acacttaacg aagaaatctt cggaattgaa cctaatgaaa gtgttgttta cgatgcaatc
    20461 gtaatgcaac gtgcgtcatt aagacaagga acacacgcgg ttaaaaaccg tagcgctgtt
    20521 cgcggcggcg gacgtaaacc atggcgtcaa aaaggaacag gtcgtgcacg tcaaggatct
    20581 atccgctcac cacaatggcg tggaggtggc gtagtctttg gaccaacacc acgttcttac
    20641 agctacaaac ttcctaaaaa agttcgtcgt ttagcaatga aatctgtatt gtcagaaaaa
    20701 gttgctgaaa acaacttggt agcaatcgaa ggcttgaact tcgacgcacc aaaaacaaaa
    20761 gaattcaaac aagttcttgc taacttatct atcgactcta aagtattggt tgttctagaa
    20821 aaaggtaacg actttgctgc tttatcagca cgtaacttac caaacgtttc tgtagtaact
    20881 tctgataatg tgagtgtgct agacgttgta tcaaatacaa aagtgttagc aacacaaact
    20941 gctcttactc aaattgagga ggtgcttgca taatgaactt actagacgta atcaaacgcc
    21001 cagtgatcac tgaaaaatca atgcttgcca tggatgacaa gaaatacact tttgaagtgg
    21061 acactcgcgc aaacaaaact ttagtaaaac aagcggtaga agctgctttt gacgttaaag
    21121 tcaaaaacgt gaacatcgtg aacgtacgtg cgaaattcaa acgtatgggc aaatatgcag
    21181 gatatacaaa aaaacgtcgc aaagcgatcg ttacattgac tgaagattca aaagaaatcc
    21241 aattattcga agctgctgaa taagcactaa tagataactc attaaattag gagggaaaaa
    21301 cgtggcgatt aaaaagtaca aacctacctc aaatggtcgt cgtaacatga caggttctga
    21361 ttttgctgaa atcacaacat caacacctga aaaatcattg ttacaaccat tgaaaaacca
    21421 tgctggacgt aacaacaacg gccgcatcac tgtacgtcat caaggtggcg gacacaaacg
    21481 ccaatatcgc gtgatcgact tcaaacgtaa caaagataac gttgttgcaa ctgttcaaac
    21541 gatcgaatat gatccaaacc gttcagctaa catcgcgtta gtacactatg aagatggagt
    21601 gaaagcttac atcttagcac ctaaaggact agaagtgggt atgaaacttg tttcaggtcc
    21661 agaagcagat atcaaagtcg gaaatgctct tccattagaa aacattccag ttggtactgt
    21721 tatccacaac attgaaatga aacctggtaa aggtggacaa ttgatccgtt ctgctggtac
    21781 aagtgctcaa gtacttggta aagaaggcaa atacgtattg atccgcttga actctggtga
    21841 agttcgtatg atcttagctg catgccgtgc tacaatcggt tctgtaggta acgaacaaca
    21901 cgaattaatc aacatcggta aagctggacg ctctcgttgg atgcgtaaac gcccaactgt
    21961 acgtggtagc gtaatgaacc ctaatgatca cccacacggt ggtggtgaag gtaaagctcc
    22021 tatcggacgt aaagcaccag tttctccttg gggtcaacca gctcttggtt acaaaactcg
    22081 taacaaaaaa gctaaatcag acaaacttat tgttcgtcgt cgcaaaaata aataattact
    22141 ttgaccatgt gtcgcacatt ttgagaggag gttcaccatg ggtcgtagtt taaagaaagg
    22201 accttttgtc gatgagcatt taatgaaaaa agttgaagct caagcagggg ttgaaaagaa
    22261 aaaagttatc aaaacttggt cacgccgttc aacagttttc ccaagttttg ttggatatac
    22321 catcgcagtt tatgatggac gtaaacatgt accagtttac atccaagaag acatggtagg
    22381 acacaaatta ggtgaatttg cacctacaag aacttatcgt ggacatgctg ctgacgataa
    22441 gaaaacgaaa cgctaatttg aggggaggat aaatccaaat ggcagaacaa atcacatcag
    22501 ctaaagcaac tgcaaaaaca gttcgcactt cacctcgaaa agcccgttta gtaattgacc
    22561 ttatcagagg taaaagtgtc gcagatgcaa tttcaatctt gaaattcaca ccaaacaaat
    22621 ctgctggaat cattgaaaaa gtattgatgt cagcagttgc gaatgcagaa aataactttg
    22681 acttagatgt tgaaaacttg gtagtaagcg aagcttttgt taacgaagga cctacaatga
    22741 aacgtttccg tcctcgtgca aaaggatcag cttctccaat caacaaacgt acaagtcata
    22801 tcacggtagt cgtatcagaa aaataaggag ggataatcag tgggtcaaaa agtacatcca
    22861 attggaatgc gtgtaggcat catccgcgac tgggatgcta aatggtatgc tgaaaaagag
    22921 tatgctgagt tcttacacga agatttaaga atccgtaaat ttatttcaac taaacttgct
    22981 gatgctgctg tatcaacagt tgaaatcgaa cgcgcaacag gtcgcgtaaa catttcaatt
    23041 cacacagcta aaccaggtat ggttattggt aaaggcggtt ctgaagtcga aagcctaaga
    23101 aaagaattaa acaaattaac tggtaaaaga atccatatca atatcgtgga aatcaaaaaa
    23161 ccagatctag atgcaaaatt agtaggtgaa ggaatcgcac gtcaattaga aaaccgtgtt
    23221 gctttccgtc gcgctcaaaa acaagccatc caacgctcaa tgcgtgctgg cgctcaaggg
    23281 atcaaaactc aagtatctgg tcgtctaaac ggagcagata tcgctcgttc agaaggatac
    23341 tcagaaggaa ctgttcctct tcatacttta cgtgcggaca ttgattacgc atgggaagaa
    23401 gcagatacaa catacggaaa actaggagtt aaagtgtgga tctatcgtgg agaaattctt
    23461 ccaactaaaa aaaacactga gaaaggaggg aaataatcat gttagtacct aaacgtgtaa
    23521 aacaccgtcg tgaattccgc ggaaaaatgc gcggggaagc taaaggcgga aaagaagtag
    23581 cattcggtga atacggtttg caagctgttg attcacattg gatcacaaac cgccaaatcg
    23641 aagctgctcg tatcgcaatg actcgttaca tgaaacgtgg tgggaaagta tggattaaaa
    23701 ttttccctca caaatcttat actgccaaag caattggggt acgtatgggt tctggtaaag
    23761 gggcacctga aggatgggtt gcaccagtaa aacgtggtaa aatcatgttt gaaatcgcag
    23821 gcgttcctga agaagtagct cgtgaagcgt tacgtctagc ttctcacaaa ttaccaatga
    23881 aaactaagat cgtaaaacgt gaggaaatgg gtggtgaatc gaatgaaggt taaagaaatc
    23941 agagaattaa ccactgccga aatgcttgat caagaaaaac aattaaaaga agaattgttt
    24001 aatcttagat tccaattagc aacaggtcaa ttagaaaaca ctgcacgtat taaagaagta
    24061 cgtaaatcga ttgcacgcat caaaactgtg ttgcgtgaac aagctaagta attatggaag
    24121 gaggccatta agcgtatgac tgaaggaaga aatcaacgca aagtttatca aggtcgcgtg
    24181 gtttcagaca aaatggataa aacaattacc gttgctgtcg aaacaaagaa aaaccaccct
    24241 atctatggca aacgcatgaa ctattctaag aaatacaaag tgcatgatga aaacaacaca
    24301 gcaaaagttg gcgacatcgt gagaattatg gaaactcgtc cattatcagc tacaaaacgt
    24361 ttccgtttgt tggaagtagt cgaagaagca gttattatct aataacacga gattttccta
    24421 tatagattga atgcaaaatc tgaaaggagg atacacaacg tgatccaagc agaaagtcgt
    24481 ttaaaagtcg ctgataactc aggcgcacgt gaaattttga cgatcaaagt ccttggtgga
    24541 tctggtcgta aaactgctaa catcggtgat gtgatcgttg ctacggttaa acaagcaacg
    24601 ccaggtgggg ttgtcaaaaa aggtgacgtc gttaaagccg ttatcgttcg aacaaaatca
    24661 ggagctcgtc gtacagacgg ttcatatatt aaatttgatg aaaatgctgc agtgattatt
    24721 cgtgatgata aaagccctcg tggaacacgt atctttgggc cagttgcacg tgaactacgt
    24781 gaaaacaact tcatgaagat cgtttcacta gcaccagaag ttttataatc ttgagacact
    24841 tatcaaagga ggtgcgaaac agttatgttt gttaaaaaag gcgataaagt taaagttatc
    24901 actggaaaag acaaaaacaa agaaggcgtt gtactagcag cgttcccgaa aaaagataaa
    24961 gttattgtcg aaggtgtaaa tgtcgttaaa aaacaccaaa aaccttctca agctgctcct
    25021 caaggtggaa ttgttgagat ggaagcgcca attcatgttt ctaacgtgat ggtggttgac
    25081 tctactggtg tagctggccg agttggctac aaagaagtag acggcaaaaa agtccgtgtt
    25141 tctaaaaaaa ccggtgaagt tttagataaa taaaagttta aggaaggagg gtcttactga
    25201 atgaaccgcc taaaagaaaa atatcttaaa gaagtaactc catcattgat ggaaaaattt
    25261 aactatagct ctgttatgca aacacctaaa gttgaaaaaa tcgtcatcaa catgggtgtt
    25321 ggtgatgcgg tatcaaacgc taaaaactta gataaagcag ttgaagaatt aactttgatc
    25381 actggtcaaa aaccaatgat tacaaaagct aaaaaatcaa tcgctggatt ccgtttacgt
    25441 gaaggaatgc caattggtgc aaaagttaca ttgcgcggag aaagaatgta cgaattctta
    25501 gacaaattgg tttctgtttc actaccacgt gtacgtgact tccatggtgt aagtaaaaaa
    25561 gcctttgacg gtcgtggaaa ctatacttta ggtattaaag aacaattgat cttcccagaa
    25621 gtagattatg atttagtaga taaagtacgt ggtatggaca tcgttattgt aacaacagca
    25681 aactctgatg aagaatctcg tgagttgttg acacaattag gcatgccatt ccaaaaataa
    25741 ttaaaggagg cgaactacgt ggctaaaaaa tcaatgattg ctaaaaacaa acgtcctgca
    25801 aaattttcaa cacaagctta tactcgttgc gaacgttgcg gacgtccaca ttcagtttat
    25861 cgtaagttcc atctttgccg tatttgcttc cgcgaacttg cctataaagg tcaaattccc
    25921 ggcgtgaaga aagctagctg gtaaaaagta aattcgcata agaaggaggt aaaagatcaa
    25981 tggtcatgac agatccaatt gcagattttc taactcgtat ccgtaacgca aacatggtta
    26041 aacatgagtc tttagaagta cctgcatcaa aaattaaacg tgacatcgct gaaatcttga
    26101 aacgtgaagg tttcgttcgt gatgttgaat acatcgaaga tgacaaacaa ggcgtgatcc
    26161 gtgttttcct aaaatttggt aaaaacggcg aacgtgttat cacaaactta aaacgtatct
    26221 ctaaaccagg tttgcgtgct tatgtcaaag ctgacgaagt accaaaagta ttgaacggtt
    26281 taggtatcgc aatcatttcg acttctgaag gtgttgtcac tgataaagaa gctcgtgaaa
    26341 aaaatatcgg cggcgaagtt atcgcttacg tatggtaatc aagtagaatc tacaaggagg
    26401 tgtctttaag tgagccgtat cggaaataaa gtcgttgtta tccctgaagg agtaacagtt
    26461 actcaagacg gaaacaacgt tacagttaaa ggaccaaaag gtgaattgac tcgtactttt
    26521 tcagcagata tcaaaatgaa tatcgaagga aacgaagtaa ctttcactcg tccaaacgac
    26581 agtaaagaaa tgaaaacaat tcatggaaca actcgtgcga acttcaacaa catggttgta
    26641 ggtgtcagcg aaggtttcca aaaagcgtta gaactaatcg gggttgggta ccgtgcacaa
    26701 ttacaaggat caaaacttgt attgaacgtt ggttactcac atccagtaga aatcacacca
    26761 ccagctggag taactgttga agtaccagct aacacacaag taattgttaa aggtgcgaac
    26821 aaagaagaag ttggcgaatt agctgcaaac attcgtggaa ctcgtcctcc agaaccttat
    26881 aaaggtaaag gtattcgtta tgttggcgaa tatgtacgcc gtaaagaagg taaaactggt
    26941 aaataatcgt ttagaaaaat tgcatagaag ccagcagcag gaaaaaggca tttggctgaa
    27001 gtttaccttc aaaaaaatac atttttactt gtgccgcagc ttctattttt cgaaacgtat
    27061 ttactgcttg aaatagtagt acaataaaaa taaagaggtg acaattgtga ttacaaaacc
    27121 agataagaac aaaacacgtc aaaagagaca tcgtcgtgta cgtaacaata tctctggtac
    27181 tgctgagcgc ccacgcttga acgtttttcg ttctaacaaa aacatctacg ctcaaattat
    27241 tgatgacgta gcgggtgtaa cgctagcaag tgcctctacc ttggataaag aaatttcagg
    27301 tggaacaaaa acagaagcag cacaagctgt cggtaaacta gttgctgaac gtgcagctga
    27361 aaaaggcatt aaagtagtag tctttgaccg tggtggatac ctttaccatg gccgtgtgca
    27421 agctttagct gaagctgctc gcgaaaatgg actagaattt tagaagaagg aggaatacca
    27481 ttcatggttt atatcgatcc aaaacatttg gaattagaag accgcgttgt tgcaattaac
    27541 cgtgtaacaa aagttgttaa aggtggacgt cgtctacgtt ttgctgcttt agttgttgtc
    27601 ggtgacaaaa acggacacgt agggtttggt acaggtaaag ctcaagaagt tcctgaagca
    27661 atccgtaaag caattgaaga tgcgaagaaa aacttagttg aagtacctat ggttggttca
    27721 acaatcccac acgaagtaat cggtgctttt agtggtggta gaatcctaat gaaacctgct
    27781 gtagaaggtt ctggggttgc tgctggtgga ccagttcgtg ccgtattaga attagctggg
    27841 gtagcagata tcacttctaa atctttaggc tcaaatacac caatcaacgt tgttcgcgca
    27901 acagttgaag gtttgaaaca attgaaacgt gcagaagaag ttgcagaact tcgcggcaaa
    27961 tcagttgaag aattaatcgg ataaggagga caacaataat ggctgaatta aaagttactt
    28021 taaaacgcag tattatcgga cgtcctcaaa accaacgcga tacagttaaa gcgttaggtc
    28081 ttacaaagat taacagttca gtggttaagc cttctaatga agcaattaaa ggcatgatca
    28141 atactgtttc tcacctagtg gacgttgaag aaatttaatt tagaaaaatc ataactagaa
    28201 cattaaggag gtgccaaacc tatgaaactt catgaattaa aacctgctga aggttcacgc
    28261 caagtacgta accgtgttgg tcgtggtact tcatctggta acggaaaaac tgccggacgc
    28321 ggacaaaaag gacaaaaagc tcgttcaggc ggcggtgtgc gtttaggatt tgaaggggga
    28381 caaacaccat tgttccgtcg tttaccaaaa cgaggattca caaacgtgaa ccgtaaagac
    28441 tatgcagtca ttaatcttga tgtattgaac cgctttgaag acggagctga agtaactcct
    28501 gttgctttag ttgaagctgg aatcgtgaaa aacgaaaaag ctggaatcaa agtattagct
    28561 aatggggaac taaacaagaa attaactgtg aaagcagcaa aattctctaa agcagctcaa
    28621 gaagcaattg aagcggctgg tggatcaatc gaggtgatct aatgtttaga ctattaaaag
    28681 atgcttttaa agtaaaagac attagatcga aaatcctatt tactgtattt gtgttgtttg
    28741 tatttcgcct aggtgcacat attactgtac caggcgtaaa tgctgcttca ttgcaaaacc
    28801 tttcttcttt gccatttttc aacatgttga acttggtaag tggtagtgcg atgcaaaact
    28861 tttccatttt ctcaatgggg gtttcaccat acattactgc ttctatcgtc attcagcttc
    28921 ttcaaatgga catcgtgcct cgatttgttg aatggtcgaa acaaggggaa gttggtcgta
    28981 aaaaactgaa tcaagcgact cgatatctga cattagtatt ggcatttatc caatctgtcg
    29041 gtatcactgc tggattcaat caatacgctc aattaggttt cgtgaataat cctaacgtag
    29101 caacttacgt aacgatcggt ttgattctaa cagccggtac gatgtttgtt acttggctag
    29161 gagaacagat cacagaaaaa ggtatcggaa atggcgtatc aatgatcatt tttgcaggta
    29221 tcatttctag attgcctgaa agtatcagag aaatttatga agattacttt ataaatattg
    29281 atccttcaga catatggaaa tctgctattt ttgtggccat tttactagta gcggtcttgt
    29341 tgatcgtgac attcgtaaca tttttccaac aagctgaacg caagatcccg atccaatata
    29401 cgaaacgcgt agctggcgcg cctacaagca gctatctgcc gttgaaagta aatgcagctg
    29461 gagttattcc agttatcttt gctagttcgt ttattgctac gccaaatgct gttttacaag
    29521 cattgagcgg tagattcggt tctgaaaact ggtacaaagt gatgatggag atctttaact
    29581 ataatacagc acctggtgct acgatttata cgattctgat cgttgccttc actttcttct
    29641 acgcctttgt tcaagtaaac cctgagaaat tagcggaaaa cttgcaaaag caaggaagtt
    29701 atataccaag cgtacgccct ggtaagggga ctgaagagta tgtctcaaga ttactgatgc
    29761 gtttaagtac agttggtgca gtcttccttg gtttagtggc attgcttcca atcattgctc
    29821 aaatggtttg ggatcttcca caatcaatcg gattaggcgg tacaagctta ctgatcgtta
    29881 tcggtgtagc tttagaaact gcgaaacagt tagaaggctt aatgctgaaa cgtaaatatg
    29941 ttggattcat tgagtaagaa agcgtgttga ggtttcccct caacacacgt tctcacacat
    30001 ttattaggag ggaaaacaat gaacctcatt ttaatggggc tgccaggcgc aggtaaagga
    30061 acacaagctg aaaaaatcat tgatgcttat ggtattccgc acatctcaac aggagatatg
    30121 ttccgcgcag cgatgcaaaa cgaaacagct cttggcctgg aagcaaagtc ttatatggat
    30181 aaaggtgcat tagttcctga tgaagtaaca aatggaatcg tgaaagagcg actagctgaa
    30241 ccagatacag agaaaggctt cttattagat ggctttccac gtacgctaga tcaagcaaaa
    30301 gctctggacg caatgctaaa agatttgaat aaaaaaattg atgctgtcat cgatatccat
    30361 gttggtgaag aaatcttggt cgaacgtcta gccggccgat ttatctgcag caattgtgga
    30421 gcaacttacc ataaaatttt caaccctaca aaagttgaag acacatgtga tcgttgcggt
    30481 gggcatgaat tctatcaaag agaagatgat aaacctgaga cggttaaaaa tcgtctggct
    30541 atcaacatca aaaatagtga accaatcttg gcttattata aagaacaagg tttgctaaac
    30601 actattgatg gtgctcgtga aatcgatgct gtatttgcag atgtacaaaa aatcattgaa
    30661 aaatagcacg attgcccgta gccgacttgc attatgcagt tactcttgtc aagttcaata
    30721 aattatgata gaatatgtca gtccgacttc tagagaatcc tctagatgat ccattttgag
    30781 aattttgagg tgggaactgc agtttccgcc attttatcgt gtgaaaatgc gaaacaagta
    30841 caaggaggta ctgtgcgtgg caaaagaaga tatgattgaa gtcgaaggta cagtcgtcga
    30901 aactttgccg aatgcaatgt ttaaagttga actagaaaac ggacaccaag ttcttgctac
    30961 tgtttcaggt aaaattcgta tgcattacat ccgtatctta cctggtgaca aagtaacggt
    31021 tgaattgtct ccatacgatc ttaaccgtgg ccgtattact tatcgcttta aataattgta
    31081 ctccgtaaaa ctacaggagg tattatcatg aaagtaagac catcagtaaa accaatgtgc
    31141 gaacattgta aagtaattcg tcgtaaagga cgcgttatgg tgatttgccc agcaaatcca
    31201 aaacataaac aacgtcaagg ataattggag gtgctataaa tatggctcgt attgcaggag
    31261 tagatattcc tcgtgataaa cgtgtagtag tttcacttac ttatatctat ggtattggta
    31321 acactactgc taaaaagatc ttagctgatg ctggtgtatc tgaagatgtt cgcgttcgtg
    31381 aattaacgaa tgaacaaaca gatgctatcc gtgcagaagt agataaatta aaagttgaag
    31441 gggatcttcg tcgtgaagtg aacttgaaca tcaaacgttt gatggaaatc ggttcttacc
    31501 gtggaatccg tcatcgccgt ggattgcctg ttcgtggaca aaacacgaaa aacaatgcac
    31561 gtactcgtaa aggcccagct cgcgctatcg ctggcaagaa aaaataattc ttaagtgaag
    31621 gaggttaaaa cttcatggca gcaaaaaaag tgaatcgtaa acgccgtgtg aagaagaata
    31681 tagaagcagg tattgcacat atccattcta cattcaataa cactatcatc atgatcactg
    31741 atactcacgg aaatgcatta gcgtggtcat cagctggttc attaggtttc aaaggaagca
    31801 aaaaatcaac tccttttgcc gctcaaatgg cagcagaaac tgctacaaaa gcagcaatgg
    31861 aacacggatt aaagactgtt gaagtaacag ttaaaggacc tggttctgga cgtgaagcag
    31921 caattcgttc attacaagct acaggtttag aagtgactgc aattcgtgac gtgactccag
    31981 ttcctcataa tggtgcccgc cctccaaaac gccgtcgtgt ttaatgagtg ccgctcattt
    32041 tgagttttat cagtaacgtc attgaacaag acacttttcg ttttgaaagg ggtaaagata
    32101 agaatgattg agttcgaaaa accaagaatc gcaaaaattg atgaagaaaa agattatggc
    32161 aagttcatcg ttgaacctct tgaaagaggg tatgggacta ctttagggaa ttccctacgt
    32221 cgtattttat tatcttcttt gccaggtgct gccatcacta atattcaaat cgatggtgta
    32281 ctgcatgaat tctctaccat caaaggtgtt agagaagacg tcactcaaat catcttgaac
    32341 attaaaggct tagcgctgaa aatgtatggc caagaagaaa aaacccttga aatcgatatt
    32401 accggacctg ctacagtaac tgctggcgat attatcgttg atagtgatgt agaaattctt
    32461 aacaaagata tgtacatctg tagtgtctct gaaggagcta cgttccatgc tcgcttaaca
    32521 gtgaaacctg gacgtggtta tgttcaagca gatgaaaata aaaaagaaga tatgccaatt
    32581 ggcgttcttc cagtcgattc aatctacaca cctgttcgtc gtgtgaacta tcaagttgag
    32641 aacacacgtg ttggacaccg tgaagatttt gataaattga cgatggagat ttggacagat
    32701 ggttcaattg aaccgttgga agcaatgagt ttgtctgcga aaatcatgac tgaacatttg
    32761 gatatttttg tgaacttaac tgatgaagca aaaaatgctg aaatcatggt cgaaaaagaa
    32821 gaaacacaaa aagaaaaaat gttggaaatg acgatcgaag agttagactt gtctgttcgc
    32881 tcttacaatt gtttgaaacg tgcaggaatc aatactgtac aagaacttac aaataaatct
    32941 gagcctgaaa tgatcaaagt ccgtaactta ggacgtaaat cattagaaga agttaaagca
    33001 aaattgcacg acttaggttt aggtttacgt aaagaagatt aaaacctttt acgaaggagg
    33061 gaaaccaacg tgagttatcg taaactagga cgcacatcta gccaacgtaa agcgatgttg
    33121 cgtgatttaa caactgattt attaatcaac gaacgtattg tgactactga agctcgtgcg
    33181 aaagaagttc gctcaactgc agaaaaaatg atcactttag gtaaacgcgg agatttgcat
    33241 gcacgtcgtc aagctgcggc tttcgtacgt aatgaagttg ccagcgtgcg tgaagaaaat
    33301 gaggacatcg tcattgaatc agctttacaa aaactgttca acgatattgc acctcgttac
    33361 gcagaacgtc aaggcggata cacacgcatt ttgaagacag aaccaagacg cggtgatggt
    33421 gcaccaatgg ttgtcttaga acttgtctaa aatcacacta ataacatcac tttatagatg
    33481 attcttttag agtgttatga tgttggaaga tatttttccg agtctagctc agcgctgctc
    33541 ctcaagcgaa tatcgcttgg ggagtaagca ctcatcataa agtgtttttt tataccttca
    33601 attacctgtc atgacagata tgtttgaagg cataaaaaaa cattttaagc gagaaataat
    33661 tgaaaatttt gtcttttttt gatagtgtta gtaggaactt taggatgaag aaggagtaat
    33721 catggagcca attattgaat tagaaaagat aaattataaa taccagccag atgatcttcg
    33781 ccccgcattg aaagatgtct cctttacaat tgataaaggt gagtggatcg ctattattgg
    33841 acacaatggt tcaggaaaat cgacacttgc caaaacgatc aatggattgc ttctgccaga
    33901 atcagggatc gtgaaagtgg gaaatcaaat attggatgaa gaaaatattt ggacgatccg
    33961 acagatggtt ggaatggttt ttcaaaatcc agacaatcag tttgttggtt cgacagtaga
    34021 agatgacgtt gcttttggtc ttgaaaatca agggattccc agagaagaaa tgctagtgcg
    34081 tgtgaaagat gcacttgaga aagttagaat ggctgagttt gcttcacgcg agcctgctcg
    34141 tttatcagga gggcaaaaac aacgtgttgc cattgccggc gtggttgcct tacgacctga
    34201 tatcattatt ttagatgaag caaccagtat gctagatcca gaaggtcgtg aagaagtgat
    34261 ctctacgatc aagaagatca aagaagaaag tcaattgacc gttatatcta tcactcatga
    34321 tattgatgaa gcagctaatg ccaatcggat actagtgatg agacaaggcg aacttgtccg
    34381 tgaaggtaca ccaaaagaaa ttttttctgc tggtcctgaa ctgatcgact taggtctaga
    34441 tctaccattt cctgaaaaac tgaagagcgc actgaaagaa cgtggggttg atgtgcccag
    34501 tgaatatatg acagaagaaa ggatggtgga ttggttatgg acatccgttt tgaacaagta
    34561 gacttcactt atcagccgaa tacaccattt gaacaaagag ccttatttga catcaatatg
    34621 acgatcaaag aaaacagtta tactgcatta gtagggcata ccggaagcgg gaaatcaacc
    34681 ttacttcagc atttgaatgc gcttgttaag ccaacaagcg gtacagtcca tattggagaa
    34741 cgagatattc agccggatac ggataataag aatttaaagc ccatccgaaa aaaagttggc
    34801 atcgtctttc agtttccaga agcacagcta ttcgaagaaa cggtagcaaa agacattgct
    34861 ttcggtccta aaaactttgg tgtcagcgaa gaagaagcac tagtcctagc aaaagaaaca
    34921 ttagaacaag ttgggctgga tgaaagctat ttggaacgtt cgccatttga actttcagga
    34981 gggcaaatgc gtagagtagc aatcgctggt gtgcttgcca tgaggccgga agtacttgta
    35041 ttggatgaac caacggcagg acttgatccc cagggtagaa aagaaatgat ggagatgttt
    35101 tggcgtcttc ataaggaaca gcagatcact attgtattag ttacgcattt aatggatgat
    35161 gtagccaatt ttgcagatta tgtctatgtg ttggaaaagg gacgaatcgt taatagcggg
    35221 gagccgcaag aggtctttca aaacattgaa tggctaaaag aaaaacaact aggcgtacct
    35281 actgccaccg agtttgcaga agaattaatg gctaaaggga tgaactttgc taccttgcct
    35341 ttgacagctg aagaattagc agacgctatc gtccattatg ctggaggaaa tgcccatgat
    35401 gaataaattg atatttggtc gttatatgcc tggagattcc ttgatccatc gattggatcc
    35461 ccgagctaaa ttattagcca gtttttattt tatcggaatc atttttttag caaataattg
    35521 ggaaacgtac cttttgttgg catttttcac attactttct gtcttcttgt caaaagtaga
    35581 tttgaaattt tttatccgag gcattcgccc attgatctgg ctaatcctct tcactgtagc
    35641 tttgcaggtc ttgtttacaa gtggcggaga aacttattgg agttggggtg ttttccatat
    35701 cactgaattt ggtgtgatga acggcctctt tattttctgt cgattcgtgt tgatcatttt
    35761 tatgtcgact ctactcacgc ttactacacc gcctttggaa ttatcggatg cgattgagta
    35821 cttgcttcgt ccattgaaag ccgtacggtt cccagtccac gaaatttcat tgatgctgtc
    35881 gattgctctt cgttttgtgc ctacattgat ggacgaaaca gaaaagatca tgaacgctca
    35941 gcgcgctcgc ggtgttgatt ttggtgaagg aaacctagtt cagaaaatga aagcagtcat
    36001 tcctttgctg attcctttat ttgtcagcag cttcaaccgt gcggaagatc ttgcgactgc
    36061 tatggaagca agaggctaca aaggtgggga aggaagaacg aaatatcgtg tattgcattg
    36121 gcataataga gacacgatgg ttatcattgc ttttgtatta ttaactgtgc tactgatctt
    36181 tttgagagga taaacgttca gtgggccgct atctattctt ttcttgctga tctgtttgca
    36241 agaaaggatg cgagcagtgt atgacaagaa atattagggt ctgtggcaga ctaaggtcac
    36301 aggcctcttt cttttatctg aaaagaaaga acgaaaaaat tttataaaaa aatcattatt
    36361 ccagataacc ttttaggaac aaaggagaga aaaacatggt acgttacaaa gcaatcattg
    36421 catacgacgg aacaaatttt aatggattcc aaaaacagcc aaatgggcgt actgttcaag
    36481 aagaagtaga aaaaacatta caaaaaatgg caaatggaaa agaaatcacg gtctttggtt
    36541 cgggaaggac agatgccgga gtacatgcga ttgggcaagt aatccatttt gattatccag
    36601 aggagcgtcc gttagaacga atgcgtttcg cgctagatac ccagtcacca gaggatattg
    36661 ctgtaagaca agtagaaatt gtttcagaag acttccacgc aagatatttg gtcaaagaaa
    36721 agacgtatca atttcgagtg gatataggga aaccacgcag tccttttcga cgccattatg
    36781 caagttattt tccttaccct cttgatttgg aaaaaattca gcgggcgctt cctgatttat
    36841 tagggactca cgatttcacc tctttttgtg catcaggaag ctcgatagag gataaagtac
    36901 gtacgatcta cgaggctaag atggaggtaa atgaaacaaa agacgaattg ttgtttacat
    36961 ttaggggaaa tggcttcttg tataagatga tacggatctt agtaggaacg cttttgaaaa
    37021 ttggcaatgg ccgattgcct gaagatagta tcccagatat tattgcgaaa aaagatcgta
    37081 atgcagcagg acctacagca catcctgaag gactttattt gtatgaagtg aaatatgaat
    37141 aagaggttgt gacagaagtg gtcagcttca agaaataaga aggaatttac gaaaattact
    37201 tctcaaaatt ttgcaaaatt tcggcttatt ttcgaaggag ctacttctgg aacaccgtga
    37261 atcagaggtt gtgaaaaagc tgccctgcat caagtaataa gaacaaccaa acaaaaatag
    37321 ctcgtcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    37381 ttaaggaaga ggttgtgaaa tcagcgtttt gacctgagta ttagagaaac aaacggaaaa
    37441 atagttcctc atattttgcc gtttgttgag ctaataccga aggtcagctg attgaacacc
    37501 gtttaagaaa gaggtcgtga gcctgtcatt taactctgag tcacaagtaa caatttcaaa
    37561 aaacagcttc tcatctttga tgaatatcgt gacttgtgga cgaggcgttg ataagtgaat
    37621 catctttttg ttacctattt tttctgtaac caaaaaacga tggaacgttg ttttgattgc
    37681 ccttaatgag gacagtcgga acaggtttgt catttttata tttaaatggc acaaattttt
    37741 ctaattattt gtcatttagc gctgaaaatt tttgctattt atctaaaaaa gtgataacta
    37801 cttaagatga agcagaatat ttacgccata cggcatacaa ccgcgaaaaa tataaaaaaa
    37861 agaaaacgat tgaacgcgtt tttgcagtcg caaaggaaaa gcatggaatg cgctacatca
    37921 agtatcgaga cttggaaaac aatcaggtgt atacgctgat tgtttttgct gcaatgaatc
    37981 tgaaaaagtt gtccacttgg cagttggaac atatgtcttc atatctttta ttatacaaaa
    38041 atagcaaaag caccagtcta cactcttagt aaagaggaaa ctgatccttt tgtctacatt
    38101 cttaaagtac ttaaactaag tactttattt tactctttag gattgataca gtagcatcta
    38161 tccctttatt aataatcttt tattttttt
//
LOCUS       DAYYME010000009        37995 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000009 DAYYME010000000
VERSION     DAYYME010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.009"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(159..1469)
                     /locus_tag="ACHBFW_000384"
     CDS             complement(159..1469)
                     /locus_tag="ACHBFW_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFM9316731.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="ACHBFW_000385"
     CDS             complement(1481..2899)
                     /locus_tag="ACHBFW_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9316732.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3139..4707)
                     /locus_tag="ACHBFW_000386"
     CDS             complement(3139..4707)
                     /locus_tag="ACHBFW_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFM9316733.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4711..5121)
                     /locus_tag="ACHBFW_000387"
     CDS             complement(4711..5121)
                     /locus_tag="ACHBFW_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM9316734.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5118..6041)
                     /locus_tag="ACHBFW_000388"
     CDS             complement(5118..6041)
                     /locus_tag="ACHBFW_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFM9316735.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6038..6916)
                     /locus_tag="ACHBFW_000389"
     CDS             complement(6038..6916)
                     /locus_tag="ACHBFW_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFM9316736.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6903..8240)
                     /locus_tag="ACHBFW_000390"
     CDS             complement(6903..8240)
                     /locus_tag="ACHBFW_000390"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFM9316737.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8243..9346)
                     /locus_tag="ACHBFW_000391"
     CDS             complement(8243..9346)
                     /locus_tag="ACHBFW_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM9316738.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9420..10928)
                     /locus_tag="ACHBFW_000392"
     CDS             complement(9420..10928)
                     /locus_tag="ACHBFW_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9316739.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10894..11586)
                     /locus_tag="ACHBFW_000393"
     CDS             complement(10894..11586)
                     /locus_tag="ACHBFW_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM9316740.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11608..12582)
                     /locus_tag="ACHBFW_000394"
     CDS             complement(11608..12582)
                     /locus_tag="ACHBFW_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9316741.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12882..13754
                     /locus_tag="ACHBFW_000395"
     CDS             12882..13754
                     /locus_tag="ACHBFW_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9316742.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14212..16410)
                     /locus_tag="ACHBFW_000396"
     CDS             complement(14212..16410)
                     /locus_tag="ACHBFW_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9316743.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16642..17634
                     /locus_tag="ACHBFW_000397"
     CDS             16642..17634
                     /locus_tag="ACHBFW_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9316744.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17694..19115
                     /locus_tag="ACHBFW_000398"
     CDS             17694..19115
                     /locus_tag="ACHBFW_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFM9316745.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19108..20607
                     /locus_tag="ACHBFW_000399"
     CDS             19108..20607
                     /locus_tag="ACHBFW_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFM9316746.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKLIVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20646..22040
                     /gene="uxaC"
                     /locus_tag="ACHBFW_000400"
     CDS             20646..22040
                     /gene="uxaC"
                     /locus_tag="ACHBFW_000400"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM9316747.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22199..22882
                     /locus_tag="ACHBFW_000401"
     CDS             22199..22882
                     /locus_tag="ACHBFW_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFM9316748.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22884..23720
                     /locus_tag="ACHBFW_000402"
     CDS             22884..23720
                     /locus_tag="ACHBFW_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM9316749.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23731..25485
                     /locus_tag="ACHBFW_000403"
     CDS             23731..25485
                     /locus_tag="ACHBFW_000403"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9316750.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25761..26102)
                     /locus_tag="ACHBFW_000404"
     CDS             complement(25761..26102)
                     /locus_tag="ACHBFW_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM9316751.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26170..28341)
                     /locus_tag="ACHBFW_000405"
     CDS             complement(26170..28341)
                     /locus_tag="ACHBFW_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM9316752.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28552..29409
                     /locus_tag="ACHBFW_000406"
     CDS             28552..29409
                     /locus_tag="ACHBFW_000406"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM9316753.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29480..30337)
                     /locus_tag="ACHBFW_000407"
     CDS             complement(29480..30337)
                     /locus_tag="ACHBFW_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9316754.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30322..33024)
                     /locus_tag="ACHBFW_000408"
     CDS             complement(30322..33024)
                     /locus_tag="ACHBFW_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM9316755.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33037..34326)
                     /locus_tag="ACHBFW_000409"
     CDS             complement(33037..34326)
                     /locus_tag="ACHBFW_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFM9316756.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34476..35522
                     /locus_tag="ACHBFW_000410"
     CDS             34476..35522
                     /locus_tag="ACHBFW_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9316757.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35524..37677
                     /locus_tag="ACHBFW_000411"
     CDS             35524..37677
                     /locus_tag="ACHBFW_000411"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFM9316758.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11229 a         7815 c         6764 g        12187 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc
     3121 atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc
     3181 agcataactt cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt
     3241 aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt
     3301 tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt
     3361 cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc
     3421 tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt
     3481 ggattcattt aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc
     3541 aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc
     3601 gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt
     3661 atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc
     3721 ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat
     3781 cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt
     3841 ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct
     3901 ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc
     3961 tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc
     4021 aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc
     4081 tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc
     4141 ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt
     4201 cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg
     4261 ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga
     4321 aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga
     4381 tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt
     4441 gtaaacaaga taaaacaatc catcagcata actgatttgt ggcgcccata tacttccatt
     4501 tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt
     4561 gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat
     4621 aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat
     4681 aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata
     4741 taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt
     4801 gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt
     4861 tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac
     4921 ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga
     4981 atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa
     5041 agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa
     5101 tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt
     5161 cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt
     5221 ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct
     5281 tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca
     5341 ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta
     5401 gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga
     5461 aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca
     5521 taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct
     5581 ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag
     5641 ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct
     5701 cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag
     5761 aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca
     5821 aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg
     5881 ctttctatat attcatcata tcttccttct tttactatca agacctttgg atggctatcc
     5941 ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct
     6001 atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt
     6061 gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag
     6121 aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca
     6181 gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg
     6241 gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt
     6301 aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg
     6361 ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata
     6421 agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct
     6481 ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg
     6541 ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc
     6601 aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag
     6661 aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt
     6721 gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg
     6781 ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt
     6841 gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa
     6901 aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat
     6961 tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt
     7021 ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa
     7081 aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca
     7141 tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct
     7201 aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg
     7261 atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac
     7321 ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca
     7381 ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta
     7441 gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta
     7501 cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg
     7561 caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc
     7621 ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg
     7681 catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag
     7741 ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc
     7801 aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga
     7861 ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca
     7921 tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa
     7981 gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga
     8041 tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa
     8101 aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt
     8161 gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca
     8221 aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg
     8281 caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac
     8341 catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc
     8401 agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt
     8461 gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa
     8521 caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta
     8581 cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt
     8641 ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg
     8701 ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt
     8761 ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt
     8821 ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt
     8881 tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat
     8941 ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg
     9001 gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg
     9061 tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt
     9121 caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta
     9181 tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta
     9241 cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg
     9301 ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt
     9361 taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat
     9421 tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt
     9481 gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat
     9541 tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt
     9601 gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga
     9661 ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga
     9721 gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa
     9781 ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca
     9841 tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt
     9901 acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga
     9961 taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga
    10021 ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt
    10081 cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc
    10141 aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta
    10201 tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa
    10261 taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa
    10321 ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact
    10381 tcataaagct catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt
    10441 cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg
    10501 tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt
    10561 aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt
    10621 ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt
    10681 gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt
    10741 ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt
    10801 aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg
    10861 ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct
    10921 tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc
    10981 tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc
    11041 ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat
    11101 cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc
    11161 gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat
    11221 tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct
    11281 aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata
    11341 tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt
    11401 cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata
    11461 cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa
    11521 attcaaataa accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg
    11581 cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc
    11641 aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc
    11701 tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat
    11761 ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc
    11821 taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa
    11881 tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa
    11941 ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt
    12001 ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat
    12061 tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa
    12121 tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt
    12181 ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat
    12241 tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa
    12301 tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact
    12361 tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg
    12421 aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt
    12481 attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga
    12541 tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt
    12601 gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg tatataatca
    12661 tttttaccgt aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa
    12721 agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca
    12781 cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga
    12841 aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta
    12901 taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct
    12961 gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg
    13021 cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt
    13081 ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga
    13141 tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg
    13201 gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc
    13261 caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc
    13321 ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg
    13381 atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa
    13441 ttatgctgcc attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt
    13501 actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac
    13561 agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg
    13621 ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc
    13681 ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt
    13741 ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct
    13801 tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt
    13861 atttttcgaa gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca
    13921 gccttgagcg atgataaaaa ctctcacaaa cattgcgacg agtaacccac aggagcaata
    13981 agaagcgatt ttgaaaaata gcttcccata tttcacaaaa ttatgcctta ttgtcgatag
    14041 cctgtctctt gaacgccatt tattcgtaaa aaagaactgt gacaagactt ttgtcacagt
    14101 tctttttgcg tgcttagcct tgaaatttaa ctatccataa atacaaatac tagggttatt
    14161 ctaacttctt ttattgcttg aatcagtcca caagaaaaac agcctcttga ttcacaaata
    14221 tttttttcga tattctcccg gcgtaatcga ttccttcttt ttaaagaaac gaataaaatt
    14281 ttgcggattt cgatattgca atctttctgc aatctctttt acggagaggt tagtctcttt
    14341 caaccatttt tttgctacct ctaaacgatg attttgaaca aaatcaccaa atttttctcc
    14401 tgtttccttt ttaaatacat tacttaaata gttagggtta taatgcaacc tatcagcgat
    14461 cacatctaaa gacagatcac gatcatattc tgtgtagacg atatgcataa tattctccga
    14521 caaattttta aattcatgat cagtgattgc ttgagagcat tctgtgattg gcagaactaa
    14581 ttgttctaaa acaagttttt ctagttcttt gggatgataa ttgttcagtg cttttacata
    14641 aatttgtttc atgttatcaa agttttttga atctactccc aataactgtc ctaattgaac
    14701 gagttcattg atcaagcgga ccgaaactac ttcccgactc aaaggatttt tattttgtgc
    14761 aaacaatgca tcaatcaatt catgtactaa attcttcact tcctgatttc cagaacgaat
    14821 ggcttcaaac aatcgatttt gttcttctat cggatatcta ataagtgttt tttcatgagc
    14881 agcaggaacg atttcctgat aaaaaataat tgaacttgga ccagtattta cacgataata
    14941 cagggcttct tttgcccgat caaccgattg cttgctttca ttcagcgttt cgaaccggtt
    15001 actgatgcct atactgatag ttaaattcaa gtattctttt acacgttttt gaatgagtcg
    15061 gcaatactcc atgacttttt tgcttgcttc tgcatcatct ttatccattc gataaatagt
    15121 ggcttgcatt tcttcattca aaacgatcgg cagcattcga ttttcctttg gcacaatttc
    15181 cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt gactatcttc
    15241 taacagatcg atttgaacca aagctgtata atagcagtca ttttgagaac tataaccaaa
    15301 ttgctgcaac cgctgatcta gttctcgcct ttctactcga tttctaaata aactaagtac
    15361 aaatagcgtt tccagctgtg gcttttgggt aactaagtgt gcacttaacg cttctttttc
    15421 tcctatgatc cgactgactg attgagcgac caaatctagt tctttcccac tgaatccttt
    15481 tgatttaaga ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc tgtccgaaaa
    15541 taagtaggaa agaagaccaa tgagtcctac cagtgttaaa gacactgcaa ctagcccgat
    15601 tctcagattg cggatcattg cgccaattgc actagagtca acttctaatc tgtaaatcca
    15661 attattataa tctgatttca gtactatata cgttttatct ttttctctta tcactttcag
    15721 ctgattgtaa tctagatcca cgtccgttaa tacagttggt tgctctttat tttttactgt
    15781 ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt ctccactttc
    15841 accaacaact tgttctattg tatgtttctc tatcgtagca attcccaatg catatttttc
    15901 tttttcaaac attggcaaag aaatcaccat ttcgattcct ttgtttttct tttgccaaaa
    15961 taaattattt ttgttattta tgtacttctg ttgatacttt tcaacttcct cttcggtcag
    16021 ttgactcaac gatccattga ttactcccca tttaccagcc aaactgacta aatcataatt
    16081 attatcatcc ataacaatcg tttctataaa atttagttct ttcctgattt cagaataagt
    16141 ggagtaatct gtatatgtca gcggatgctt gaatatgtcc gagaaatttt tggtcgaacc
    16201 atacgtagaa aacgaatatt caatcgttct aattttccat tcaacgttag ttttaacttg
    16261 cctgagcaaa ttctttttgt ctgttaaata actcgattcg atcgattcat aggtattctg
    16321 gtaaatgaat aaactaaatc ctatgacaag tacggctcct aataaaaaca tcggtaaaaa
    16381 tattttataa aatactgtac ttttcttcat tttcttcctc tcctcctttt aaaataaaag
    16441 gctttcattt ttctgtaaaa ttgatactca tctaattaaa tgagcattcc ccgctttcaa
    16501 gaatagcata attaaaaata aaaatccaag taatttttaa cgtgtgcaat cttttcatat
    16561 caaagatcta ttgacttatc gtagttatcg taatatgatg aatgcgttaa caaaaagtga
    16621 aaaagtaagg aaaatgatgg aatggtaacg atcaaagata tcgctaaagc agctggcgtt
    16681 tctcatacga cagtttctcg tgctttgaat aacagcagtt taataaaaaa tgaaacaaaa
    16741 caaaaaatac gacaactagc taaggaaatg aattattcgc caaactatag tgcaaaaggt
    16801 ttagtgaatc aaaaaaaata tctaatcgga ctatttttct caagtttgca taaaggaact
    16861 tcatcaaatt tcctagcaga ttccattgca ggcatccatt cagtattaga cacaaattac
    16921 agtttatctg ttgaaagtat cgatgttgtt gatttaacag aaatcaacct gcaacgctac
    16981 gatggactga tcgttatgag tcaaagtgat gatgatcagc ctttcatcga ctatctcaaa
    17041 gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca atgatccagc ggttatcaac
    17101 gtcactgccg atgatgcggc cggtgttatg caggcagtag attacggtat tggatgcgga
    17161 catacgaaga tcgcttatat tggcggaaag aaaaattttc gttcgacaaa agaacgggaa
    17221 aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg tcaatccagc ctatttttta
    17281 acaggagatt acagcttaga aagcggcctc caaaacatgg aatacctatt atcactttct
    17341 gacctgccta ctctggtttt ttgcggcaac gacgacatag cgattggtgc attgagagct
    17401 gtacatttag ctggcctccg tgttccccag aacatttctc tgatcgggtt cgatgattct
    17461 ccagtcgtct cctatctcaa tccgccgctt accaccgtac gtaagccttt gaaagaaatg
    17521 tgtcaagaag gaacaaagct tttactagat ttgatcgaag gcaggacgat cgaacaatct
    17581 aaaattgagc tctccacctt gttgatcctt cgtgagtcga tcaattactt gtaaactaca
    17641 agtaatttta tataaaaatg ttaacgtgtg cataacacag aaaggaagtt tatatgaaaa
    17701 aactgacaaa agaattagtg aaaaaaaacg aagcacctgt aaaagttctt cagtttggtg
    17761 aaggcaattt tatgcgtgga tttattgatt ggcagattca gcagctgaat aaccaacaat
    17821 tgtttaaagg taatgttgca atcattcaac ctttagaaca aggtcttgga aatatgatga
    17881 accagcaaga taatttatat acggtcattt tacaaggact cagcaatggc gaaatcgttg
    17941 atacttccga gattatcacc gtagtagaac aaatgatcaa tccttatgat gaatgggatg
    18001 cttatctatc tctagcagaa aatgacgact tggcctatat cgtctccaat acaacagagg
    18061 caggaatcca attcgtggag actgataatt taacagatac tcccccacag agcttcccgg
    18121 gcaaactgac agccttttta taccgacgtt tcaaattaaa caaagctggc tttaccatca
    18181 ttccttgcga actgattgac agaaacggcg aacgtctaaa agaaatcatt ttacgctacg
    18241 cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa aatgttttct
    18301 gttgtagttt agttgatcgc atcgtcccag gttatcctcg cgaagatgca ggtgttttca
    18361 atcaacggca taactatgaa gatcaattga tggtcaaagc agaaccattc atgctttggg
    18421 tgatcgaagg gcctaaagaa ttggagaatc aactaccatt caaacaggct ggcttgaatg
    18481 tcatcatcac agatgatatg actccttatc gtgaacgtaa ggtgcatttg cttaatggac
    18541 cgcatacagc gatggtccct cttgctttgt tggcggaatt ggaaactgta gaagaagtaa
    18601 tgaaagatcc tttattctcc gcatatgttg atcagttatt caatctagaa ttgattccta
    18661 tgctttcttt acctaaagat gaattagcca tttatgctga ccaaatcaaa gaacgtttct
    18721 tgaatccttt tgcccatcac aaattagaag cgatttcatt aaatagtgtt tcaaagttca
    18781 gcacccgtct gcttccagtg ttcaagaagt atatcgaaga acaaaatcag gtaccaccgt
    18841 taatcactgt ttcactagct gcgctacttc ttatgtatcg aggcgatcaa gtaaagccac
    18901 atgatgatga aaagacgata agcgaattta ctgatgcttg gtctgataat ggaacagcta
    18961 ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa ataccgaatg
    19021 tgacagatac agttcaagaa atagctgcaa agatcgatca aaaaggtgtc cggtcagtat
    19081 tacaagaaat gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac aatgaaatta
    19141 aatcgacgag acagtgtggt cgtcgcatta acccctattc caaaacaaac acaactgacg
    19201 attgatcagc aaacgatcac cactcttgaa gatatcccgc aaggtcataa gattgcattg
    19261 ggtgatttga aagctggaga caacgtgatc aaatatggct atccaatcgg tcatgtgacg
    19321 acagacgtaa ctgccggtca atggctgcac actcataatg taaaaaccaa tctttctggt
    19381 gaattggatt atcgatatga aaaagatgtc catccaagtc attacctttt tgagaaccga
    19441 acatttcaag gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga tttgttttta
    19501 gtacctaccg ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca gttcaaggaa
    19561 aaacatccag atctgggaca gtttgaccat attacgattt taaagcatcc ttatggctgt
    19621 tcgcagcttg gaaaagatca tcaaaatact cgagaaattt tagcggacgc agtaaatcat
    19681 cccaatgccg gaggagtttt agtatttgga ttaggttgtg agaacaatac agttcctgaa
    19741 ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga aattaatagt ggctcaagat
    19801 gtttacgatg agatcgaaca aggtgtggct ctattagaag agcttttaac agcggcggaa
    19861 aatgaccagc gcatatcagt cccgctctct aagttgaatg tcggtttgaa atgcggcggt
    19921 tctgatggtc tatcgggaat aactgctaat cctttacttg gtgcattttc tgactacttg
    19981 attgctcaag gaggcagcac gatcttaaca gaagtgccag aaatgtttgg cgcagaacaa
    20041 gtattgatgg cacgagcgga aaatgaagaa gtttttgact caatcgttga cttgatcaat
    20101 gacttcaaac agtatttctt gtcttacggg gaaccagttt acgaaaaccc atctcctggt
    20161 aataaagcag gtgggatcac tacattagaa gataagtctt taggctgcac gcaaaaatca
    20221 ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa accagggctt
    20281 agtttattgc aagctcccgg gaatgatctt gtagctgcct ctgctcttgc ttcatccgat
    20341 tgccagttgg tcttgtttac cacaggaaga gggactccgt ttggctcata tgtcccaacg
    20401 gtcaaggtat ctacgaatac aactcttttt gaacgcaaag gtcattggat ggattttaat
    20461 gcaggcgtct tattagaaca accaatggaa gtaatcttag aatcgtttgt tcaaaaaatc
    20521 atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg agaaattgcc
    20581 atttttaaaa acggagtaac actttgattt tcataaaaca gtataaaaag aaagaaggaa
    20641 gatttatgtt tttatctgat gattttttat taaaagacga ttgggcgaaa aagctttacc
    20701 attcctacgc aaaaaacatg cccattattg attatcactg tcacttagat ccaaaagaaa
    20761 tttacgaaaa caacaatttc agcaatctga cagaagcttg gttaggaggc gatcattaca
    20821 aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga aatgcttctg
    20881 attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa aattatcgga aatccgcttt
    20941 acagttggtc acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg ataaatgaag
    21001 aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt ggcagctcct gattttcgcc
    21061 gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat gatcccgcag
    21121 acgacttgca ctatcatcag ttgcttgctc atgaagaacc agatttgaag gttctacctt
    21181 cattccgtcc agataaagca ctcaatattg aaaaagaagg atttaatgca tgggtcacac
    21241 tactggaaga agcagctgac caaacgatca ccacctatca agaactgata gaagtcttag
    21301 ggcagcggat agaatacttc catcaacatg gatgtcgtgt ttccgaccac gctttagaca
    21361 tcttacgcta taaagaagcg gatgaaacag tattagaaga gatttttcaa aaagcacgag
    21421 ctaacaaact attatcttca gatgaaatcg atgcttatcg tacagaaaca ctgatacatt
    21481 tgacccactt ctatcattct cataattgga cgatgcagct gcacatccat gcttatcgta
    21541 actgtaatac acaaatgttg gaaaaattag ggcccgatac cggctatgat ggaatgaatg
    21601 atctttcttt gactattcct ctgcaaaaat tattgaaccg tgcagaagaa acggatcagt
    21661 tgccaaagac gatcctttat tcattgaatc cgaatgacta tcctgcgatt ttagctttga
    21721 tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt aggttctgga tggtggtaca
    21781 acgatactcg agctggtatg cgcgatcaat tgacacaatt tgctgatggt agtgctttgg
    21841 ggaattttgt cggcatgctg acagattcaa gaagtttcct gtcctacact agacatgaat
    21901 atttccgtcg tgttctttgt gaatttttag gcgaaatggt catgcgagga gaagcacctg
    21961 aagatgaaca acttttagga agtctagttc aagcgattag ttatacaaat gctaaaaatt
    22021 actttggctt ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa acaggatagt
    22081 gaaataacgg aatccgttta tccacgtttt agagaagaag acggaccaaa tttgctccgt
    22141 cttcttctct tgctgttctg cttcattgcc tataaacctt tatgaaagaa gagataacat
    22201 gcctactatc catattgccg gtgattctac tgcggcagaa aaagatgaaa acaagcgtcc
    22261 cgagacaggt tggggagaaa aaatctctgc ttatttttca cccgagatcc agatcaataa
    22321 ccaagcaaag aatggacgca gcaccaaatc atttatccag gaaggaaggt tagcccgtct
    22381 agcttcacag tttcagccga atgatttctt atttgtacaa tttggacata acgaccagaa
    22441 aatccaagaa gaaagaggta cgcttccctt cggtagctat ttaaaaaatc tgcagatatt
    22501 ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg tgacgcgacg
    22561 agattactta gaaaacggaa cattgaatcc agatatactt ggagactatc caaaagcgat
    22621 gcgtgcattt gctgagaaac accatattcc tcttttagat gtgttctcaa gatcacaaga
    22681 attgttccag accttctcaa aagaagagac aaaaaaattc tacctgcatc ttatacctga
    22741 tacttgtaaa aattacccag aaggactaaa agacaatacc cactttagtc cagaaggcgc
    22801 tgataaagtt gcccgaataa ttgttgaatt gataaaagaa tcccgtctgc ctcttgctaa
    22861 ttatttacaa aaaggagttt gagatgtttc cattaaattt aggcatccgt gcccacgatt
    22921 tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac ttgtcccata
    22981 tccaatttgc accacaaaaa gcatttcctg aacaacttaa attaactgac atgacacttg
    23041 ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa tatcgaactt tccattttag
    23101 gatgctatat caacctatcc agcaaaaatc aagaagttcg tgcacaagca ttggcaactt
    23161 tcaaacagca aattgctctg tgctctgcct atcaagccaa gatgattgct acggaaaccg
    23221 gtagtgtctc tgtcggttac acaaaagaaa attttactga agaagcttat cagatcgcca
    23281 gaaattctat cattgaatta gtcgctcatg cagaaaattt tggcgtaact gttgctgttg
    23341 aagcagggat caatcatcca atctataact atcagttagc taaacgttta atcgatgaag
    23401 tagcttcacc aaatttaaaa atcattttag attgtgcgaa tttgatgcat aaagaaaatc
    23461 atgctgatca ggaaaaagtg atccgaggtg ccttggacaa tttacatggt tatattacag
    23521 cgcttcatct caaagactat atcatggaaa atggaaagat ccgcatggta cctgttggca
    23581 agggctgttt agatttccgc ccggtactgc attatatcaa atatgagcgt cctctaatgt
    23641 acgccactct tgaagctact cctgagattt atgttcctga agcaatagaa cacttgcagt
    23701 ttatttacca agattgttaa ggagaagatt atggaggaac gaatacagaa aagttatcaa
    23761 aaatatcatg caaatgcttt tcagacattg ctgcatcttt atcagggaaa ttattggcgt
    23821 tttgccatgt caggactctt ttttattatc aaacatcttc catcttggtt aatgccgatc
    23881 gctattgcca atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt gcaggccatt
    23941 tatttcaacg cttttttgat ggcttgtctg attggtcaaa atatattgac gaattatttt
    24001 catgtaaaat accatagcac ttcgatccgt caagtagaag ctggactaag agcgaccctt
    24061 atcaagaaaa tccaagaact atctattcct tatcaaaaag aattgcaatc tggacgaatc
    24121 cagtcaaaga tcatccgtga tgtagaagct atccaaacgt tgtcctctca agtatttgtc
    24181 agcggattaa atatcgctgt caatttattc gtagctttag gtatcacgct ttataaaagt
    24241 cctgttgttt tcatgttttt cttattgact gttcctctcg ccgcattcgt tactctcttt
    24301 ttccgtaaaa ggatcagaca gtccaactca gacttcaggc aagaaatgga agagacttct
    24361 gcaaaagtga tcgaagtgat cgaattagca ccgattgctc gagctcatgg gttagaaaaa
    24421 cacgaaacaa ctcgcttaaa taagcatttt actcaaatct atgaaaaagg attccactta
    24481 gatatcctgc aatctgtttt cggatcagtc agctgggcta gttttcagat atttcagctg
    24541 atttgtttga ttttcactgg gatattagct ttacaagggc gcatccaacc cggcgatgtc
    24601 gtgatgtatc agacgtattt cacgacgatc gtcaattccg tttctggaat gatcacgctg
    24661 attcctacga tttcaaaagg catggaatct atcacttcta ttggcgaact gttgattgct
    24721 ggcgatgtag aaacttatca cggtaagaaa aaaatccaac atttggatgg aaattttcgt
    24781 tttgaggatg tttcttttct ttatccggac tcactcaaac cgattatcga tcatttttct
    24841 ctagaggtga aagctggaga aacaattgct tttgttgggc catctggctc tggaaaatct
    24901 acactcctta acttattgat tggatttatc cagccgactt caggaaaaat ttttctagat
    24961 gatcagcctt tggatgaact ggatatgcga agtgtgcgaa attatttagc cgttgttcct
    25021 caaaccactt tgttgttctc cgcttcgatc aaagagaaca tcacttatgg cttgaaaaac
    25081 gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc aactttcttc actgattgac
    25141 cagcttcctg atggtctgga taccttagta ggcgaacatg gcaacaagct gtctggtggc
    25201 caaaagcaac gtatctccat tgcacgagcg ttgatacgtc agccaaaaat cattttgcta
    25261 gatgaagcaa cctcagcttt agataaccag tcagagaaaa aaatccagca agccctgaat
    25321 tacctgacag aaacacccac tacttttatt gtggctcatc ggctttcaac gatcaaagag
    25381 gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg aaataggtac ttacgaaaaa
    25441 ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa aataggagct
    25501 atgacaagac ttttgtcgta gctcctattt ctgaataaac ggtgttctcc tgccaactcc
    25561 tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg ttttttgaaa ttgttccttg
    25621 taactcagag ttaaacggta ggctcacgac ctcttggtaa atggtgttca agagacagcc
    25681 tatcggcaat aagaagcaat tttgtcatcg ctcaaggccg aacgtctctt tcacaccctc
    25741 tgttgtttca taaaatagtt ttattcgctg taaaggtcgc gaactggtgt catgatgata
    25801 cggttcaagt cattgatgat tgtactgaag ccttgctctt tttgcatcaa gtcattgatt
    25861 acttcttctg attggatttt cacagccatt tcttgtgctt tcattgcatc ttcgtcgctg
    25921 aactcttgtc cactcatttg tttttcttgt aattcgattt gcatcgcttg gaagtctttg
    25981 aacagtttgt gtgcttcttc gtttgcttgt actttctcgt acgcttcttt caacgctttg
    26041 aattctggta attcacggat ctcacgttcg atttgattcg cgctatcgta gatgtttgac
    26101 atgttatgcc ctcttttctt ttagtagtta ctctctattg taacatgggg ttattcgttt
    26161 tcaaaggggc tactctcctt gatatttccg ccagaggtct ttagccgctt caccaattcc
    26221 ctgaacgcct tctttcacca tatcgccgaa gtttttcagc ccttcttttg cttgttcacc
    26281 atattgattc aagttatctt gccaatttcc agactctcca ttagaatttc ctgtgtttcc
    26341 aacttgatct gcagctacca cttgtcctcc tgtggcatag gcatctgcta cactaaatcc
    26401 tgtttttgaa gtatacggaa ggatgccttc tgccgctgat ttgaagactt tcccgacaac
    26461 atttccactt gtcccttcca aatagtgcag cttgctggat tcttcgtaac ccatccatgt
    26521 cgaaatcact acgtctggcg tgtaaccgat catccattgg tcatttgctt ttgtggcatc
    26581 gaaattagtt tctgtggttc ctgttttccc tgctatcgta taacctgcag gattcgcagc
    26641 agccgctgtt ccatttgaaa atgtaccaag aagcatgctg gtcatttcat cggctgtttc
    26701 ctttgatatg acctgttttg gttttttgtt tgtgttatcg acgatcaccg ctccagtcga
    26761 atcaacgatt ttcgtgataa gatgcggcgt atacatctga ccatcattgg caaatgcact
    26821 gtaagcaccc gccatgatca atggagatac tcctttttcc agtccaccaa gggctaagcc
    26881 ataatactta tcagattttg ctaaaggcag gccgaattct tctgctttat catatccttt
    26941 ttgtaaaccg atctcatgca aaagccatac agtaggcaaa ttcaaacttt gtgcaacagc
    27001 ttgatacatc ggtacttctc cttggtatgt cccgtcaaaa ttatgcgcat cgtaataact
    27061 ttgcggttcg tctttcaaga tactggaagg cttgtatcca gcttccagtg ctggtgtata
    27121 tacgcttaat ggtttgatcg ttgatcctgg tgaacgacgc atctgtgtag caaagctgaa
    27181 tcctctaaag acatgatctc ctcttcgtcc tactagtgct tgtacaccac ctgtattcgg
    27241 atctaaagct actgaagcag attggaccat ggcaccatct gaagcatttg ctgggaataa
    27301 tgcactgttt tgataagtag cttctaattg cttttgataa tcctgatcta aagctgtata
    27361 gattttatag cctttattca tgacatcttc ttcatcaaga ccatactcgt taactgcttc
    27421 atcgatcact gcatcaaaat aataaggata acgataacca gaatctgaat tatcgtatgt
    27481 gtcattaaga tacgcgctga tatctgtctg gctttctgct tgttcttgtt cttttgtgat
    27541 ttttccgttt tcttccatta cacttagtac tgtgtttttc cgatcattcg cattttttgg
    27601 gtgatcgatc ggattataaa taccaggacc tttcagcatt cctgccaaag tagcagcctc
    27661 agacagcgta acattcgttg catctactcc aaaatacttt ctggaagcgt cttggacacc
    27721 ccataccccg ttgccaaaat aggcattgtt aagatacatg gtcaagattt cttctttgct
    27781 gtacttcttt tcgatctcga ttgccaaaaa taattccttc gcttttcgat caaacgtttg
    27841 gtctaatgtc agatacgcat ttttggcaag ctgctgagtg atcgtacttc ctccgccccc
    27901 tgatgttccg ccagacaaga ccattcggac tgctgcacgg gcaatccctt tgatatcaaa
    27961 cccgtgatgc tcgtaaaaat tcctatcttc tgtcgagatt actgcatctt ggatataggg
    28021 agaaatcgcg tttaattcta catatgttcc tttttgggcg tacagtgtgc cagcttcttc
    28081 gtttttctta tcataaatga ctgtcgattg gcttaatcct gattttaacg tttcgacatt
    28141 tgcttgtttt gctaaaataa agaggtagat acttgtcact aagacacaga tcaaaccaat
    28201 cagcaaaatc aatttgttca catgatattt tttccagatg cgctttcgcc atcgatggaa
    28261 ttgtccgaga tatggtctga tccaacgcca aaaactgatc aaagcagctt ttatcttttc
    28321 taaaatgctt tgaaaatcca tttgcttctt ctcctgtcta tttgattcaa tcgtcttatt
    28381 ttaccatagt gaaagagtgt tttcttcctg cttaagttgt agttcaatat gtttttttac
    28441 gttagtgcaa gtagagtaac ataagaatga acagaaaaat gtaagttttt tcttaaatat
    28501 gttttaaaaa tggtacaatt agtctgcgat tgcactgaaa ggagtacact tgtgaaaatc
    28561 acgatcactt tacctatgaa ccatcctgaa acaacaattc gcgacttgct tgaaaaggaa
    28621 tggttagttc ctagaaaggt tcgacacttt ttgcgtatac gtaaaaacgt ttggatcaat
    28681 caacaacctg ctttgttcca tcatgaagta aagggcaatg accagatcac attgttattc
    28741 gaagaaacag attataatta tcaagaagtt caattaggag atgcgaagca catccgtgtt
    28801 ttatatgaag atgagcattt gattatagtg gataagcctg caggaatgaa gactcatcca
    28861 aatgaaccga cagaaaacag taccttactc aatcatttag ctgcttatct cgacactaaa
    28921 cagcaacgac cttacgttgt ccaccgcttg gacaaagaaa caagcggtgc cgttttattt
    28981 gcaaaaaatc catttgtctt gccgatactt ggaagaatgt tggaacaaaa aatgatctat
    29041 cggagatatc aagccatcgt atggggagaa attgccaaag atatgaccat caccaataaa
    29101 attggacgag atcgccatga tcggagaaaa agggtcgtag atgataaaaa tggtcagagc
    29161 gccttgacac atgtcactgt caacaaagta ctttctgaac aaacacaagt ctattgtgtt
    29221 ttggaaaccg gacgtacaca ccagatcaga gttcatttag ctcataaggg acatccgatc
    29281 gttggagacc ctctctacca aaatagaaca gctgaacgac tgatgctcca tgcattagaa
    29341 ctgcacatga tccatccatt tactcaagaa aaaatagtcg gtaaagcttt acctggatta
    29401 tgggaataga aataaaaaag aacagagtct tatcagcaga taatgataag actctgttct
    29461 ttttgtgtaa tttgtctgct tacttttgat gttcgtgttc tgctaacagc aaactaccga
    29521 taatcccact ttcattcggc agtccccaac gaacgatata ttcacttact tgaggtgttt
    29581 ccatataacc acccatcaat tcaacaaatt gctgacggat tttttgaagg agatgatcct
    29641 gattcattac cccaccgcct aaaataatct tttcaggtgc taacgtcaac gtatagttga
    29701 tcagtgcttg agcaatataa taagcctgaa tagcccaaac aggatgatct tgcggcagat
    29761 cttgcccttt gatccctgtg cgtgcctcaa tagacggtcc tgctgctaat ccttctaaac
    29821 aatctttatg ataaggacaa gtaccttcat aatggtcatc tggatggcgt ttcacccaaa
    29881 tatgtcccat ttcaggatgt gcgatccctg aaaaaatttc ttcattgatc acgacacctg
    29941 caccgattcc tgttcccacg gtaaggtaaa tacaactatt ttttccttgt gctgctcctt
    30001 gcttcaattc cccatatgca gcagcattga catccgttgt ccaagcagca ggaatattgt
    30061 agcgttcctt gattgtgcca agaaaatcga attgtgccca cccttttttg ggagttgcca
    30121 aaacgtatcc gtaattatct tttgttttgt cgataccaat cgggccgaat gagccaatcc
    30181 cgattccctt caattcataa cgatcaaaaa attcaaatac ctgatccaat gtttcttctg
    30241 gtgttgtggt agggatactt aatttttcta aaatttctcc accatctgaa accgcacaca
    30301 caaattttgt tccgcctgct tctattccgc catacattcg ttttcctcct cgaatccgat
    30361 tgtccgaatc tcataaggtt ggattattgg gacaaccgct tgcttcgttg gttcttcaag
    30421 cagattcaat atgacagttt ttccattgtc tttagttatt tccaattttt ctaaatgact
    30481 agacatattg aaacctctta ctactacttt atcactaaac ttgctgcgtt tcaaagctgt
    30541 aattgcaaac gtttcacttt ttatcgttaa atattgttgt tttgaaatca atgttccttc
    30601 atgatgtttg atttgttgag tggaaaaagg aatctgtgct gcgtatgcat gcaaataagt
    30661 agagaatttt tcttctggtc catgtagttc aatgctgtaa ttgaaccgat gctcacctag
    30721 acactgtgct tctggtgttg gaaaatatcc ccaatcgcct agttctccca cactgcgaag
    30781 tagcgtcact gcgatttgtc cgtcttctgt tacttcatat tcattcaaac caaagtttcc
    30841 gactgttaca ccgtactctt catcatgaat attgacaaat gcttgctgat gttgcggatt
    30901 cgtggggttt tcccaacttt tagacacatg gtttgggcgc ttcaccactt caaaaatact
    30961 atctgcttcg tgatgttcca catgtaactt tgtaggaaac aagacacgta agcgatgatc
    31021 tttcatctga ttgtcgagag tcgtttcaaa gtcgattttt ttactatctt ttctgattgt
    31081 catgattgtt tttatttgaa ggattcgttt ttctttggat cgttctgctt ttcgataacg
    31141 aaattccaca accatctgct gttctttttc taaacgctcg tctgcagaaa cagggatttc
    31201 catttcctgt agaatactta tttttgctat ttccggtgta tctactaata cttttacttc
    31261 agagttttct ttgttactgg ataggattgg ctgatcacag aaaggttgtt taaagatata
    31321 ctcatttcct atatcgccga tatcttcaaa aatatgcaga ttattcaact ttcgtttgtt
    31381 tttgcgatct tccattgtaa tggtaccatt tttttcaatc gtcaaatgta gagggccgtt
    31441 ttcgatgatt cgtccactct gatgaatcat tgtttccttt tcttctgctt tcgcttcccc
    31501 ttctacaagc gcgaatgttt cccaagaaaa tgctggcatg ttttctaatg gtaaagtcac
    31561 tttgatcatt cttgccatat atggcacacg gaaacggtct ttcggcaaat cataaccaaa
    31621 ttggacagtt tcttcagata aaatagctgg tatagtggcc ccactttttg tttctacatg
    31681 atacttacgt ttaggcaaat tttctaactc ttgatatagt tgttcaggta ttccttcagc
    31741 gaatcttttt ctttctaatt cgatggttat ttctgcttca ccagttttat ccattcctgc
    31801 agtatttaca atgacaaatg gaaagctgtc ttttgggaat ccggtcgtat cgattgctgc
    31861 ggtcagctgt tctaaggaat cttgtgcaag atactttcca acttcatctg ctttttcaaa
    31921 acgaggtatc atttcccgat ggacagaatc gactgaacaa ccgcaaatac tgtcgtgagg
    31981 atgattttgc attaatgtct tccaagcata atctaattga tcatggggat agtttccact
    32041 aacttcataa gccatcgtcg caagtggttc tgtaatattt tctagctgtc gctggacctt
    32101 ggtattccat tgttttaagt aaacccgagc agatgctgta ttggctaaag tataccagcc
    32161 atccgtttct tgacttgtca attctccttc tactgagcct aagtcttctg gcacatcttt
    32221 ttgaactgct tccagatagt cagtaaaatt agaatgaata aactcgtaat ctggaaacag
    32281 ttcatttgcg agatggattg cttttgatat atctttttgt accggttgat gatcaacacc
    32341 attcatcatc aataagtggt tcgtagaggc atattgttct gcatccgcta atttttgctt
    32401 ccaaaaggca agagctgctt ctttttctac tggaatctca ttcccgttgc tataccaatt
    32461 ggcaaatagc agaccaaaga ttgctgtttg atcagggcct ttccaccaca tctctgagta
    32521 ttgggaagaa tagttttctg cttccaaaac ttgattatca aagccaattg gttttacacc
    32581 acggccaaac gctgctgctg aaataccagc ttgtttcatt aattgaggcg tctgtcccat
    32641 atttccaaat gtatcgggaa aatagcctaa catgacaggg gttccccatt tacggctttc
    32701 ttccatgcca atcagcatat ttctcacgtt cgattccgag ctgataagaa aatcatcttg
    32761 caagatataa aatggtccaa ttctcaattt tccttcattg attgcttgtt ggattgcctg
    32821 tcttttttct ggccttactt gaagatagtc atctaaaata atcgtttgcc catccaaatg
    32881 aaaactgttg aaggacggat caacttcaaa tagctctagt agatcgtcta tcaattcaat
    32941 cagtcgcatg tgatgctgtt cgtaagccat gtaccattca cgatcccagt ggctatgaga
    33001 aatgatatat actttctttt tcatttacga tacctcctac ttttctactc taatatcaaa
    33061 gtaatccatg actaattcgc aaaacatcat atttgcccaa gaaaaccatt ctctcgtata
    33121 attttcagga ttgttcacat cgaatccttc gtgcataagg tgtgttcctg cgtcagttgc
    33181 tacaagtaaa ttcaatatcc gctctttttc agatttgtct gacgttgtca ttccttccat
    33241 agctaatgca atcggccaaa cgtatttttc tggcgtatgg gaggatccaa ttccttttgc
    33301 gtactttcct tcatagaaat atggattttc tttactcaac aatgttcttc ttgtttgcag
    33361 ataatcttcc tcttgttcag aacaatatcc caagtaaggt gcggcgatca gattgggcac
    33421 attactgtca tccatgactg tggcgtttcc cattccatct acttcatatg cataaatcgt
    33481 ttgccctttt tgattaaccg ttcgtccgtg attctgaatc ccctcttgga tctctttctt
    33541 caatttctgt gtttgctgta caatttcttt atcatcgagt atcgtttgaa aaatttcttc
    33601 cacataacct aacacgacca ccgcaaacat attggatggt accagatatc cataacggca
    33661 agcatcatcg ctaggacgga aaccagacca tgtcattcct gtaaaagctg tttttgcacc
    33721 tcgcccgtca ttgatcagcg tatcttcttt tcttgtcgtg tctcgtacaa atgtataagg
    33781 agagtgttca tgatcttgct ctgttttgaa cacgtccaag atttttttga taccttcaat
    33841 aaaagattga ttgaattgat cagtttttcc tgtatttttg taaagaagat aggccaactg
    33901 tattgggtag cataaagaat caatttcata ttttctttcc cagatccaat cgttcatttt
    33961 tgtatgatct gtttggtggc cagcaccgtt agcttcttcg ttgaaggcat tcgcatatgg
    34021 atcgatattg atataaaaaa attgcttttt gactaacccg ctgatcatgt cagctaattc
    34081 ctcatcttct tttgcaatca ctaaataagg acgaacttgt gcagtagagt ctcttaacca
    34141 catcgctgga atgtctcccg tcaataaaaa ggtggttccg tcattttgct ttttgactgt
    34201 tgtcaacaga gtattggcaa atgctgctgt aaaattcttt gcccagtccg catggatatc
    34261 cccacatttt tgtgtgatat cttccataaa tttctgcacc gattttggta tatctttata
    34321 aaccactaat aaaaccttct ttctatttga tatatcttta aagattatac ttcatacagc
    34381 caattatgtc tacgctttca aaaaatatct ttttatttac tttttcatcc attatggtat
    34441 tatttgaata tgatatatca ttaagaggtg tttatatgac tcaaccattg taccagacaa
    34501 tatttaatga tttgcaaacg gctattttaa ctggtgaatt acctgtagac agccaactac
    34561 caactgaaaa agaactttca cgtacctata aagtaagcag aatcacttcc aaacgtgcat
    34621 tgactgaatt agagcaatta ggacttattt atcgcgttcg cgggaaaggc agtttcgtta
    34681 aatccgcttc tccggaacag attcttgctt ctcctacaaa aacacgacgg attctgtttc
    34741 ttcttccata tgtcgctgat ttgtctgttg gtgattttag caagggattg aaccctgtga
    34801 tgcaagataa acagattgat gtcatgatga cgacgcttga ctttctacaa aataaaacag
    34861 caaaagagct aatcaatgaa tttgacggat tgatttatta tgctcttgat acagaacaac
    34921 atcttgactt actttttgag ctttcattga aagaattccc tgtcgtcata ttagataaaa
    34981 aaatctatga gttacctttt cctaccgttc tttccgataa tttccaaggc ggtgcgcttg
    35041 ctgcctctta tttgatcgaa cagggacatt caaaaatcgc ttatttgttt ggtacgcaga
    35101 ctcatccaca atccgttcga cagcgttacc ttggttacct tgaagcattg gacaaagctg
    35161 gtttgacttt ccacacttca ctggatgaca aaagcgcgac attagatgaa ttgctttctt
    35221 atatcactaa gtatgaagta actgcttttg tttgtgaaaa cgatgttgtc gctatccaga
    35281 ccatgaatct cttgagacag catggttatc atatccctga agatttttca attatcggat
    35341 ttgatgatat ccaagcagca gctcttgttg acccgccact gacaaccatc gcacaagatt
    35401 ttgaacagtt aggtaagctt gcgggagatg ctttggtcac ttggttagag acaaaagatc
    35461 gaccatccga tagtaaattt ccagtttcat tcatcaaaag acaatcaaca aaggagaaat
    35521 aaaatgaata tcggtcaaat agatactaga catgcaacag cgaatcagcc aaatttctct
    35581 aatgggaact gccagcctta tactggcgtt ccttttggga tgaattattt tgcaccgcaa
    35641 actacggatc aaaacggcag ctggtggttt catcctgatg accgcgtttt tcaaggctac
    35701 cgtcttaccc atcaaccaag tccttggatg ggtgatttca gtcatatgct gtttacgccg
    35761 atcaatggaa aactacagga gaacaccctt ttccatgctc aaagttctta ccgcccagaa
    35821 gaaagtatat tttgcccgac tcatctgtcg atctgtcaat tacgttatgg catctgttct
    35881 acattgatac ctagcatgta tggcggaatc ttatctattg attacagcaa aaatgacagc
    35941 ggtttacttt tatcctttcc gggacgccat cagctctttg ttatagatcc ttacacagtg
    36001 gaaggaaaaa tcacacattt tgctggttca gaagatcctg atttttcttt ttattttata
    36061 ttgagatttg aatatccttt aacaattgat gagttagctg tttccaacaa aaatagcgac
    36121 tcgatcccgt tgtattttga acagacaaaa aaacaaacta tccgatttgg gacatctttt
    36181 atcagctcgg aacaagcagc ttataacctg gaactagaac aaaaccaaag caaagagact
    36241 taccttgctc acagtcaaga acaatggcag tcctattttg atcgtataca gctcacacac
    36301 catgatcaac aacaattgcg gacgttttat cataatttat atcgggtgtt tttatttcca
    36361 caaacatttt atgaaattga tcctagtggg caaaaaatcc actatgatac gactagtcga
    36421 tctataaaac ctggtgttct ctatacaaat aatgggtttt gggatacgta taaaacggtt
    36481 tatcctcttt attctttgat tgctgtagaa aaatacgaag aaatgctgga aggttttttg
    36541 aacagctata gagaaagtgg ctatttgcca aaatggctgt ctcctgatga acgtggactt
    36601 atgcctggga cattgatcga tgcggtcatt gcagatgctg ctgtaaaaga tatccgacga
    36661 gacttgatgc ctgaatttct aacggcaatg gaaaaaggtg ctacggttca aagcaaacat
    36721 ccaaattatg gacgccaagg aacagaagat tacttgacat atggctatgt tcctaatcac
    36781 taccacgaat cagtcaatca cacattagac tatgcttata gcgatttttg tattagccaa
    36841 gttgccaagg tactagaaga agaggaaatg acgaagaaat atcagcagca atcattgaac
    36901 tatcaaaata tctttgacta tgagtcagga tttatgcgtt caaaagataa agatggcaag
    36961 ttcagagaac cattttcagc taatcgttgg ggacaagatt atgcagaagg aagtgcttgg
    37021 caaagcagct ttgctgttta tcacgatttt tcaggactga tcggaaaata tggtggcagt
    37081 gagagatata aagaaaaact cattgagctg tgcaatcagc ccccaacgtt tgacccagga
    37141 ggatatggtt ttgagatcca cgaaatgagt gagatggccg cgatagaatt tggacaatta
    37201 gctatttcaa accagccaag ttttcactat ccttacttgt tcagttatat aggacaacct
    37261 gaaatggcac agccactgct taaacagttg atgactcaga ccttcgacga ttctccaatg
    37321 ggttaccctg gagatgagga caatggaagt atggccgctt ggtatatctt caatagtctg
    37381 ggattttatc ctgtcacgcc tggaaccggt gaatatgtta tcggtatgcc attaattgac
    37441 aaggctgttt tgcttttgtc aaatggaaag gaattgacga ttgaagcaac gcctaatcgg
    37501 ccgcagcaac aatttatcca taaaatcaaa cggatggaca gagaatatac gaaattatat
    37561 tttacccatc aagatttgct gaaaggcggc acgatctctt accaattagg gatcatccct
    37621 caccctaaga aatatcaagc aaaagatttg cctttttcct tatcaaacta taattaacaa
    37681 gaggttgtga aaaagctgtc ctgcatcaag taataagaac agccaagcaa aaatagatcc
    37741 tcatattttt cgtttggttg gtcttattac cgcagatgca agcttttgaa caccgtttat
    37801 tgagaggttg tgaaatcagc gttctgacct gagtattaga gagacaaatg gcaaaatagt
    37861 tcctcatatt ttgccatttt ttgagctaat accgagggtc agctgattga acaccattta
    37921 gtggagaggt cgtgaaaaag ctgtttagtt tcgagtatta agaaaaattt tgtgaaaatg
    37981 gctttccaca ttttt
//
LOCUS       DAYYME010000010        37357 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000010 DAYYME010000000
VERSION     DAYYME010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.010"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..541
                     /locus_tag="ACHBFW_000412"
     CDS             20..541
                     /locus_tag="ACHBFW_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9316759.1"
                     /translation="MHIGDKIHKLRRLNNETMNDLVKQTGLSKSWVSDIESGKKQRAD
                     FNKICAIAKHYKVSLEYFQDDYLDSDEHEDIIPLTDETRVKSVKSLEQSLKYLNNKQE
                     PNLLEIKLKDTDSVPEVYYKGQKLEGLIDVSYHYHTTTDVVAGSTNDINMEYLGEVTK
                     YPDIKTIGHKRDV"
     gene            638..859
                     /locus_tag="ACHBFW_000413"
     CDS             638..859
                     /locus_tag="ACHBFW_000413"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM9316760.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(1016..1180)
                     /locus_tag="ACHBFW_000414"
     CDS             complement(1016..1180)
                     /locus_tag="ACHBFW_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFM9316761.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGREVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            1283..1804
                     /locus_tag="ACHBFW_000415"
     CDS             1283..1804
                     /locus_tag="ACHBFW_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM9316762.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            1791..2231
                     /locus_tag="ACHBFW_000416"
                     /pseudo
     CDS             1791..2231
                     /locus_tag="ACHBFW_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(2271..2429)
                     /locus_tag="ACHBFW_000417"
     CDS             complement(2271..2429)
                     /locus_tag="ACHBFW_000417"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFM9316763.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            2522..3283
                     /locus_tag="ACHBFW_000418"
     CDS             2522..3283
                     /locus_tag="ACHBFW_000418"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM9316764.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            3470..4822
                     /locus_tag="ACHBFW_000419"
                     /pseudo
     CDS             3470..4822
                     /locus_tag="ACHBFW_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            4838..6058
                     /locus_tag="ACHBFW_000420"
     CDS             4838..6058
                     /locus_tag="ACHBFW_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM9316765.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDAEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALIKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGGNEGLKVKFASNVDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGSNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTNSSKSVAENG"
     gene            6093..6770
                     /locus_tag="ACHBFW_000421"
     CDS             6093..6770
                     /locus_tag="ACHBFW_000421"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM9316766.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGIDCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAETPQEETLLQKKLKA
                     LNGGKNE"
     gene            6767..7963
                     /locus_tag="ACHBFW_000422"
     CDS             6767..7963
                     /locus_tag="ACHBFW_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010905933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM9316767.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRQFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     INPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSNWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIINVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTSIANE
                     VPVTNG"
     gene            7978..8301
                     /locus_tag="ACHBFW_000423"
     CDS             7978..8301
                     /locus_tag="ACHBFW_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM9316768.1"
                     /translation="MILDPKMDLDEIKNALKIDTDDDDVEVRRAVQAAIAYIRGAIGN
                     DKPSFYKQENDTVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKARYL
                     LFVEE"
     gene            8305..8640
                     /locus_tag="ACHBFW_000424"
     CDS             8305..8640
                     /locus_tag="ACHBFW_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM9316769.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVRTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            8637..9032
                     /locus_tag="ACHBFW_000425"
     CDS             8637..9032
                     /locus_tag="ACHBFW_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM9316770.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEKLQQNT
                     PYWDGTKSNGKRGSYMQEHAKDHVVTSSVKNGLIEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            9029..9400
                     /locus_tag="ACHBFW_000426"
     CDS             9029..9400
                     /locus_tag="ACHBFW_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316771.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EIFGNYEDFASNNPLTIQFNVQVDVWVSTLKEVDAFYFALDEIMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            9416..9976
                     /locus_tag="ACHBFW_000427"
     CDS             9416..9976
                     /locus_tag="ACHBFW_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM9316772.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGVNVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLAKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEEMTLQKFKDLVNNKAGE"
     gene            10103..10414
                     /locus_tag="ACHBFW_000428"
     CDS             10103..10414
                     /locus_tag="ACHBFW_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316773.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     R"
     gene            10438..10629
                     /locus_tag="ACHBFW_000429"
     CDS             10438..10629
                     /locus_tag="ACHBFW_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316774.1"
                     /translation="MDQIYKDLIESGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            10629..14474
                     /locus_tag="ACHBFW_000430"
     CDS             10629..14474
                     /locus_tag="ACHBFW_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFM9316775.1"
                     /translation="MAGKGQPAGNIKLGISLDSTNFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKKIEDFGDKSTRSARKLDGLKDKLGSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIDKVGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NKVSDAIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGADKLEEWKVPQYAKGTPNGGHPGGPMMVNDGRGAE
                     AVITPNGQAFIPRGRNVVLNAPKGTHVLTAEETAYMTGNKAPRYRYAKGTGFFGNLWN
                     NVKGFAGDVGNKLKDVVGDVWDFVTDPGALARKVLNGLGVLEGLVKYPLDVGKGILSK
                     ATEALTNKITELFSSGSLDTSMGMQGVYKYLADVAVAVMKKFPGFVATSGYRPGDPYS
                     HGKRNAIDIALPGVTGGSPRYTEAANYAFEKFANKIGYVITNGKVRDRSGQSGTGIHN
                     DWRPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTAN
                     LNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFDP
                     LSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAKR
                     SRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKGN
                     SRTDRDLALDIQKILVRRM"
     gene            14475..15347
                     /locus_tag="ACHBFW_000431"
     CDS             14475..15347
                     /locus_tag="ACHBFW_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFM9316776.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSENVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            15344..16393
                     /locus_tag="ACHBFW_000432"
     CDS             15344..16393
                     /locus_tag="ACHBFW_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316777.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGADEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            16397..17221
                     /locus_tag="ACHBFW_000433"
     CDS             16397..17221
                     /locus_tag="ACHBFW_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316778.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVTSTGSTVSISKISDSYELTLS
                     KGEILWSGTALMHDTQTVQPSKKMWQTVSGFLLVWLPYENGQAIGDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            17239..19371
                     /locus_tag="ACHBFW_000434"
     CDS             17239..19371
                     /locus_tag="ACHBFW_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM9316779.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKTNDRIDQTNQQIGDLGKLKKMYSNSIDFGDYD
                     YSGNPNLSAKLNASSFSLGTGATVADDNDEIVFTLDGTNQLSKYASKAQVPVAEGKQY
                     TISCEIMLEDGFTGDPSGIRLQHAYLPGGVVVLQTETVPKNELNTWQTLIGTRTVKYT
                     SDVPNEWYPLFRDIRTLKPTGKVRLRKIKIEEGSTATPYQPNLLDDPYWLGKAPLGEN
                     IANKSVTFPIKSSAYSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRY
                     GNLEPVEGLVDTWGLTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGNTRTP
                     NISEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEI
                     NSQLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSA
                     KLYREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQL
                     SIGADGAIKWRRNSSYGSDYTFAITYTI"
     gene            19388..19537
                     /locus_tag="ACHBFW_000435"
     CDS             19388..19537
                     /locus_tag="ACHBFW_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316780.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPILNWKPKFNY
                     WEGNK"
     gene            19534..19980
                     /locus_tag="ACHBFW_000436"
     CDS             19534..19980
                     /locus_tag="ACHBFW_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316781.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            19982..20119
                     /locus_tag="ACHBFW_000437"
     CDS             19982..20119
                     /locus_tag="ACHBFW_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM9316782.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            20156..20449
                     /locus_tag="ACHBFW_000438"
     CDS             20156..20449
                     /locus_tag="ACHBFW_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316783.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERSR"
     gene            20472..20669
                     /locus_tag="ACHBFW_000439"
     CDS             20472..20669
                     /locus_tag="ACHBFW_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM9316784.1"
                     /translation="MILPDKYYQVIKWTVLTVLPALSVLVATLGKAYGWNGTDMAVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            20680..21699
                     /locus_tag="ACHBFW_000440"
     CDS             20680..21699
                     /locus_tag="ACHBFW_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM9316785.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGVNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            21956..22603
                     /locus_tag="ACHBFW_000441"
     CDS             21956..22603
                     /locus_tag="ACHBFW_000441"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM9316786.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            complement(22928..23074)
                     /locus_tag="ACHBFW_000442"
                     /pseudo
     CDS             complement(22928..23074)
                     /locus_tag="ACHBFW_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            23313..24005
                     /locus_tag="ACHBFW_000443"
     CDS             23313..24005
                     /locus_tag="ACHBFW_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM9316787.1"
                     /translation="MNKKIVVSGLIIGGLVLAGIAGKNFADDILLWGGEDNIEQINHN
                     LESLDKALENKEQNISSLNSRLSSNNQELEQAKANVEEYKQKVLTLENDKNQLTIEKT
                     NLENQLSGKNAELQGKQNEINAKIDEINQKIAEINQLNTDWNNRLQTEQNKFNDAQNT
                     VNSLNQQINQLSNEKNTIKNQLQSTEQKLEKAQSENSNLNNYIEKLEKAKSDVEDTAN
                     RSQEIVTEHTGK"
     gene            complement(24287..24359)
                     /locus_tag="ACHBFW_000444"
     tRNA            complement(24287..24359)
                     /locus_tag="ACHBFW_000444"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24324..24326),aa:Lys,seq:ctt)
     gene            24740..25309
                     /locus_tag="ACHBFW_000445"
     CDS             24740..25309
                     /locus_tag="ACHBFW_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFM9316788.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            25296..26156
                     /locus_tag="ACHBFW_000446"
     CDS             25296..26156
                     /locus_tag="ACHBFW_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9316789.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            26346..27050
                     /gene="yycF"
                     /locus_tag="ACHBFW_000447"
     CDS             26346..27050
                     /gene="yycF"
                     /locus_tag="ACHBFW_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFM9316790.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            27055..28890
                     /gene="walK"
                     /locus_tag="ACHBFW_000448"
     CDS             27055..28890
                     /gene="walK"
                     /locus_tag="ACHBFW_000448"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFM9316791.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            28887..30203
                     /gene="yycH"
                     /locus_tag="ACHBFW_000449"
     CDS             28887..30203
                     /gene="yycH"
                     /locus_tag="ACHBFW_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFM9316792.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            30204..31073
                     /locus_tag="ACHBFW_000450"
     CDS             30204..31073
                     /locus_tag="ACHBFW_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFM9316793.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            31128..31937
                     /locus_tag="ACHBFW_000451"
     CDS             31128..31937
                     /locus_tag="ACHBFW_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM9316794.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(32040..32669)
                     /locus_tag="ACHBFW_000452"
     CDS             complement(32040..32669)
                     /locus_tag="ACHBFW_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM9316795.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            32839..34131
                     /locus_tag="ACHBFW_000453"
     CDS             32839..34131
                     /locus_tag="ACHBFW_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9316796.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(34207..34869)
                     /locus_tag="ACHBFW_000454"
     CDS             complement(34207..34869)
                     /locus_tag="ACHBFW_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM9316797.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            35094..35378
                     /gene="groES"
                     /locus_tag="ACHBFW_000455"
     CDS             35094..35378
                     /gene="groES"
                     /locus_tag="ACHBFW_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFM9316798.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            35429..37054
                     /gene="groL"
                     /locus_tag="ACHBFW_000456"
     CDS             35429..37054
                     /gene="groL"
                     /locus_tag="ACHBFW_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFM9316799.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT        12894 a         6308 c         8099 g        10056 t
ORIGIN      
        1 ggattaagga ggaagagtaa tgcatatagg agataagata cataaattga gacggcttaa
       61 taacgaaaca atgaacgact tagttaaaca aaccggactg tcaaaatcat gggtatctga
      121 tattgagtct ggaaaaaagc aacgcgctga ctttaataag atatgtgcaa tagccaaaca
      181 ttataaagtt agcttagaat actttcagga tgattatttg gatagtgatg aacacgagga
      241 tattattcct ttgacagacg aaacaagagt taaatcagtg aaatcgcttg aacaaagcct
      301 aaagtatctg aataacaaac aagaacctaa ccttctagag atcaaactaa aagatactga
      361 ctcagtacca gaggtttatt acaaaggtca gaagttagag ggattgatag atgtaagcta
      421 tcactaccat acaacaactg atgtggtagc tggttcaaca aacgacatca atatggagta
      481 cctaggtgaa gtaactaagt atccagatat aaaaacaatc ggacacaaga gagatgtgta
      541 aatgaaagaa gctagaccta gagacgagat agacaaacta tacaagacca aacgatggcg
      601 agacctaagg caagtagtaa tagctaggga cttcggcatg tgccaagagt gcaagcgtcg
      661 agggcggaac acaaggggca cgatcatcca tcacatagtc gaggcgaggg aagacctgtc
      721 actgttctgg tccgtagata accttgaatg tatctgtgta gcttgtcaca acagagagca
      781 tccagagaga tcaggcggga agaagaaacc aaaacctaaa tcacatatcg ttaaaatgta
      841 ttcaactcct gaaagataag tttgcagcga aatgaaggta gcccccctac tctaaaagat
      901 taaagagtaa ggcttgagaa gaacggtgct gtccttcctt cgtaaaaaga ccgcttttca
      961 agttttttgg agaaaaagga aaaagccgac caatttaagc cggctttgga cgaagctatt
     1021 tcttagtcca tttgtttcct ttttgagaag taggaggtaa tcggtcgcct ggatcaattt
     1081 ttacttctcg tccgccttgg acatttccac cacgaggtcc cacttcttta taggttcctt
     1141 ttggtttatt gtcttcgccg ggtttataga gttctcccat aggaatccct ccctaaaaaa
     1201 atttcggcac agcactgccg ataacttaat tataaggatt gtgataacga tttcaatata
     1261 tcttttgaaa gaaggtgata ttatgccgca accagcgaag agtgcaaaat tacaattatt
     1321 aaacggaaac ccaaataaga agaataccga agaactccgc aagcgagcgg ccgcagaaga
     1381 caaattaaaa atggctactg acaaaatcaa accgccgtta tggctagatt cgctaggaaa
     1441 ggataccttt gagtttatcg ctgatgaatt gctgtctgtg gatttaatca gtaatccgga
     1501 cgtccataca atggctctct actccaattg gtattcgcaa tacgtttctt tagaaaaaca
     1561 gcttcgaaaa ctacaacgag agtacaagtt gaactatgcg cttgcgaaaa aggaggcaga
     1621 ggcgagaggt gagccgttta atgaacctaa tgaattaatt ggtaatccgc tctctcggca
     1681 gatggataca gcgtcgcgga atctccgttc ttttggcgct gatttaggac tatcaccagc
     1741 agccagagct aagttagcta ttaagatggc tgatgatggt ggtgatgacg atgacgactt
     1801 ctaatatttt ggatatgtcc tacacagaac gtgtggacta ttggcaaagc tatcttgagg
     1861 agcaagcttc ttggggtggc tttttaaaat gtccatatcc ggaattgtta actacttggt
     1921 atgcggaacg attaatcgat ggaagcatac cagccagcaa agaaaatatt caagctgcta
     1981 aacggcatat gcgtgatttg cagcgccaag gaacagatga ttttccttgg atctttgacg
     2041 aagaaaaagg tcaccggcct attagatata tcgaaaaaaa atgtaaacca actgaaggcg
     2101 actttggttc gtttgttttg caaccttggc agcatttcat aattggatcc atgtacggat
     2161 gggtacatcg tgatacagga gagcgtcgct tccgcgaggc tcttattttt gttggacgta
     2221 aaaacggtaa gttttgccgt ttaaaaatcg ggcaaaatcg gtgaaaacct ttatttttgg
     2281 ctttctttta gtaataattc gatagcttta tcaagcaatt tagacatagg aaccatagtt
     2341 tcttccgaca ttttttttaa tccttcgtat aaatctttat caattgcgtt agaaattcta
     2401 attctgtttt ttagtccgta tttgtccatg tgtgacactc ctttttattt tactataaca
     2461 tccgattcca ccgcttgcaa ctaccatcaa ttgatggtag tatataaaag aggtgagtag
     2521 attgaaaaaa tacattattt acaaacactt aaataaaata aatggcaaaa tttatatcgg
     2581 tgtcacaaat gatattggca gaaggtggag gagcggtggg attgaatata aaccaccaaa
     2641 gaacgaaacc caacaccaca gaagtttttg gaatgcaata caaaaatacg ggtgggaaaa
     2701 ttttgatcat ttgattattg aagaagactt aacgatgaaa gaagcttttg agaaagaaaa
     2761 gttttatatt gaactttatg actcaactaa taagaaaaaa ggttataaca tcgcaaaagg
     2821 cggaaatggc ggaataatat ataaagttca ccccaaagga atgctaggga aaaaacaatc
     2881 taaagaattt tcttcaaatc acagtaaatg ggcaaagaac cataagaata attgtatgac
     2941 aaacggtgat gttgtatggg gtgttactca cgaacaccca aaaggcatgt tgggaaagca
     3001 ccacagcaaa gaatcaatta tgaaaaagaa agcatatagt ggtgaaaatg ctgtaacctc
     3061 aaaaccaatt gtcgcaattg aacgggatgg aactaaaaga gaatttcaca gcgctaagct
     3121 ttgcatggct tactattcga ttagtacatg tgttttttac cgacttttaa aagatggcgc
     3181 cccttatgtg ataaatccta aagcgaacta cagaaataaa gaaaaaatat tagctatcga
     3241 aggaattatg ttcaaacata aagaagatac cgaggtaagt tagtagattg cgaaaggtta
     3301 ctaactaccg taacgcgtag gagttgaata aatataatgc tcccaagagt gtccgacagc
     3361 agataatttg ctgaaaatgt acgctaaact gggccagaac tgactgaccg atgaaaatga
     3421 gggtgacctc cagagtgcga gataaaaagc tcgcagataa taacaatcga aaacatcact
     3481 aatttcaggc ctttccacat acatggtcgc ttatgatgat gaacaaggcg ccaacgttta
     3541 cgtattggca aatgctcgtg atcaagcaag cttgttgttt gataaggccg cagaaatggt
     3601 caaacaatcg ccggcgctct ttaagaaatt tggtaagcct aaacgatcaa gtattaatta
     3661 tgctcccgcc ttttctaaaa tggaaccacg cgcctcagat agccggaaat tggatgggct
     3721 aaacactcac tttggtattt ttgacgagat ccacgagttt acgaattaca atctgatcaa
     3781 cgttatcaag aaatcaagag gaaccagaaa acagcctctg atagtttata tcacaactgc
     3841 tggatatgta ttagatggtc cgttgatgtc ttattttgag caaggtgtgg actgtttgga
     3901 acatttggaa gatgacatcg atgaacggac tttctattat ctggcaaaac ttgacagtgc
     3961 ggaagaggct gatgacccaa gattatggat caaagccaat ccgaatattt gtctaatgaa
     4021 ttttgttggc atgctagatg actatgttaa ggataaaaaa gatccaaaag aatatgctga
     4081 ctggattacc aagcaattta acttgttttc cgatatcgat gagctgtcat ttgtcgatat
     4141 gcctaccatt aaacgaaaca ataaaaccat cgatattgaa acgctcaaag gtaagaagtg
     4201 tgtcggtggt tttgacttgt ccgaaacgga agactttacc gcagccgttt tagaatttcc
     4261 gcttgaaaca ggcgaggtat tcattttgca acacacatgg atcccacaag ctagatttga
     4321 tcgagataac aatcaagagc gtatcaaagc gtgggagaag gtgggagatc taacgattat
     4381 tcctggtgat tacgtcaatt atgaatacgt cttaaattgg tttgtagaaa attcgaaaat
     4441 ctatgacatt gtaaaaatca attatgacaa ggccaaggcg ctacgattaa ataaagaact
     4501 agaaaatgca ggatttgaaa ccaacgagat tcggcaaggg tttctatcat taggtgggcc
     4561 aatgcaaaac ttcaaggaaa tgctattgga cggtaaggtg attttcaaca attccaagct
     4621 ttaccgatgg tatctatcca acgtcaagct ggtgatggat cgcaactcaa actggatgcc
     4681 gtctaagcag tccaagagta gaaaaataga tggttttgca gcaagtttga acagccacgc
     4741 cgaagtgttg aatatgttgg ttaatcctgt cggaaccggg aaagtaacct attactcgat
     4801 ttccgattta atgaatatgt aagaaaggtg tggaggaatg agtattttag atcgtttgcg
     4861 ttcttttggc cgagcgaagc cgaaagcgag caaacaagag tattttttga atgacccggg
     4921 attgataccg tatttagtcg gaaaagatga aatatcagaa gggatttttt ccgtaattag
     4981 ccgtgtatcg aacgtttttg cgtctctccc tctcaaaatg atagatgcgg agtttggcca
     5041 accggacgac tgtcctgcat acaacttgtt gagcgaaggc cctcgatatt ttacaaagtt
     5101 tgattttttc cgggacgtgg aagttttgag aaactaccaa gggaatgcgt atgtgcagat
     5161 tttccgaaat atcaatggag aagtagcaga tatggcgtta ataaaacctg gtgcttgcca
     5221 tccagtgatt gatatggata gcggggagct ttactaccaa gtaactgcga ctgacaaagg
     5281 cagttataag caagttattt atgtacatta catggaaatg ctccacttta aacaaccgag
     5341 gtttggtggc ttggaaggta cagaccccac aaaagtatta acgaataccc tcggatatga
     5401 tcgagaagtc cgaaaaatct ctttaagtca gcttaaagga ggtaatgaag ggctaaaagt
     5461 taagtttgct agcaatgtgg atgaagaagc taaaaaagct acagttaaaa acattgctga
     5521 tttttatcga caaaacggtg gactacttgt ggaagaaaac ggtgtagaaa tcgaacgttt
     5581 acaacgagag ctggtagaca gcaagctttt agatactgat aaagtatctc gctccagaat
     5641 cgcgatggtc tacaacgtgc cggaacattt cattgggagt aaccagtcga gttactcttc
     5701 acaggaacag ctcaatatgg agtttttgac atacaatcta gtaccgaccg ttaatcaata
     5761 tgaagcggaa ctaaataaga aaacactatc gagaaccgaa aaagcaaaag gttatcgata
     5821 taagtttaat gtcgcaagtt tgctaagagc tgacactcag gcaagggggc aattctatca
     5881 aatcatgcga cgaggcggag catactcagc gaatgatgtc cgaagatttg aggacttgca
     5941 accgataaat aaaaccggta tggatgatta tcatatttcc ggagacctat atccaatcga
     6001 tatggatcca acattaagaa aaacaaactc gtctaaaagc gtagccgaaa acggttaggc
     6061 ttttttagtt tgcaccgaag ggaggtgaaa ggatgaaaaa agtgacgtta agcggcgatg
     6121 tcgtggataa cgataccgcg tggttatatg actggtttgg gattgattgt atctcaccag
     6181 ggaaaatttc tgccgctctt acagaagcag cgggggatga agtagaactt gatatctcat
     6241 ctaacggtgg ggatgtccta gcggcaagcg aaatatatac cgctatccgt gcctatccgg
     6301 gaaaggtatc cggaaatgtt gtgagtattg cagcaagcgc tgcaagtgta atcgcttgtg
     6361 cttgcgaacc gcttagaatc tcgccaacag cacacatcat gattcacaac gcatgggtga
     6421 ccactagcgg caacgccgag gaattaaaag ccaatgcgga aatgttaagc agtgtggatg
     6481 agtctatcgt taatgcttac gagatcaaga caggactcga ccggaaaaag cttgctgatt
     6541 taatggcaaa agatacttgg ttaaacgcac aaacagcagt ggcggaaggt tttgcggatg
     6601 aaattatgtt tgcagaagca ccagtaacgg tactcaatgc ctctcaaccg gttattccaa
     6661 aaaacgcggt aactaagttg aaaaatctaa tactcaaagc ggaaacaccg caagaagaaa
     6721 cgctcttgca gaaaaaacta aaagccttaa atggagggaa aaacgaatga atttagaaca
     6781 attaaaaaat gcgtgggtcg aggcgggaag taaagtctct gatttaaacg cgcaactcaa
     6841 tgcagcattg gttgacgatg aaaaaacaga agaagatgta gtaagtttgc aagcacaagt
     6901 aaaagcagca cgggctaaac gggacggatt gaaagagcaa gtggcaaata tggaagccga
     6961 acaagtctta aacgtcaaaa aagaaccatt agataaaaaa gatgaaaact tgaaaaacaa
     7021 gtttatcaaa gactttaaag cgatggtcaa tggtgatcct gctattatgg ctactttgac
     7081 atctaatacg gatgaatctg gtaatgctat cggattgact attcctgtag atgtgcaaac
     7141 gactattcat actttggttc gtcagtttga ctctttgcaa gaatacgtaa acgttgaaaa
     7201 agtgaccact actagcggtt ctcgggttta tgaaaaatgg tctgatatta acccactgac
     7261 cgctttggat actgaagacg gtgaaatccc agcaaatgat gatcctgcac tttacttgat
     7321 caaatacttg atcaaacgct acgcaggtat ttctacagta actaacagct tgctaaaaga
     7381 tactgccgaa aacattttgg catggttgtc taactggatc gcgaaaaaag tagttgttac
     7441 tcgcaataca aaaatcttgg cagctattga tggaatcaaa gcagcgcaaa agaaagatgt
     7501 tacagatgtt gatgggatta aagatatcat aaacgtccaa cttgatccag ctatcgaagc
     7561 tacatctatg tttattacaa accaagatgg cttcaatgtt ttagataaag tgaaacgtgc
     7621 tgatggatct tacttgttac aaaaagacgt agcttctgca actggatata ccttcttagg
     7681 taaaccaatc aagaaaattg cttctcgttt cttgccaaac aaaggtacgc aagctactcc
     7741 taaatatcca ctgtatattg gtgatctgaa agaagctgtt acattgtatg atcgcgaaaa
     7801 catgagcttg ctgacaacga atattggcgg gggggctttt gaaacagaca ccaccaaagt
     7861 acgggttatc gatcgatttg atgtgcagtt agtcgatgat gaagcagttg ttttagctac
     7921 atttacatct atcgcaaacg aagtgccagt aacaaacggt taaggagctg atttcttatg
     7981 attcttgatc ctaaaatgga tttagacgaa atcaaaaacg cactaaagat tgataccgat
     8041 gatgacgatg tggaagtaag acgtgcggtg caagctgcaa ttgcatacat tagaggggct
     8101 atcgggaatg ataagccctc tttttataag caagaaaatg acacggttga tctgattaat
     8161 ttagctattc tgcaattagc agatcattat tacaacgctc gatcagcaac cgtgagtggg
     8221 aacttgcgag agtacgattt aggttttaca agcctaatct tgcaactcaa agcaagatat
     8281 ttgctttttg tggaggagta gcgtatgccc cttattcaaa caggaaattt aaatcaacgc
     8341 atcaagtttg tccgagatac gacggtcaag gacgaggacg ggcaagttgt ccggacttct
     8401 acaaccattc ttacttgctg ggcaagtgtg cagacacaac gcctgaacga tattaagacg
     8461 tccattggta cggctttgga aggaacactg acgttcatta tccgctatca acaaaaatca
     8521 gagctaacca atgatatgaa agtgcgttgg aatggaaaaa cgtttgaaat cattacgatt
     8581 acgaaaggcg agtttgcgaa ggacttcacg acaatcattg caaaagaggt ttcaaaatga
     8641 gtgtagaagt cgatgcaacc gaagtgtaca aagcgcttag ggaagtaaaa gcaaacgttc
     8701 aacgagtgga aagcccagca cttagaaaag ctggggagta cgctcaagaa aagttacaac
     8761 aaaacacacc ttactgggat ggaacgaagt caaacggtaa acgtggttcg tatatgcaag
     8821 aacatgctaa ggaccatgtg gttacaagct cggtaaaaaa cggattgata gaagtcggct
     8881 atgataaaga tgtttcttgg cggatgcact ttatcgagtt cggaacaatc aaacaacgtc
     8941 caaaaggttt cgtacaaaaa acacaaaagc aaatcgaaaa acaagtaaca caaatcattg
     9001 ctgacgaagt aaaaaggagg ctaggacttt gaaaacggca gtatcacaag tctattcaat
     9061 tctgaatagc aatgaaaaaa caaagaacat tgatttttac accaatagtg ttccggaatc
     9121 ggctcaaaca gtacctagcc ttccagttgg cagaattaca gagatattcg gcaactatga
     9181 agatttcgca agcaacaatc ctttgaccat tcaatttaac gtacaggtag atgtatgggt
     9241 atcgacctta aaagaggttg atgcctttta ttttgccctt gatgagatta tgagggggaa
     9301 tggttggcaa tgcgcataca cggaacaaac agatgatgag gatttagaag gtgcaaagcg
     9361 gattatcaaa cgatatgtag caaatatttc actaaattaa aaggagagaa aatagatggc
     9421 aacagtagga tttgagagcg tcatttttgg cgtaaaaaca ggtgcaggcg gcactctaaa
     9481 agaattagta gcagataagt cgaaaggcgg agcgatcgaa gctaaaatta ctggattagg
     9541 cgcaacttct aacacaacat acgcttcaaa cgtaccattc ttcattgcaa gtaaaggggt
     9601 ttcgtcgcca aaagttacgc ttgacgtggc agacttaatg gataacggca tttacagcga
     9661 aatcattggc gctaaaaccg tggatggtgt aaatgtaatt ggttcagaaa ctgaagcgcc
     9721 ttacgtgtcg gtagtcatgg ttacagcgaa caaagaagga aaacgcttat tcatgggatt
     9781 ggcaaaagga aaattcagtc atccagatat cgacatgaaa acagctgaag acaaaggagt
     9841 tgaattgcaa accgattcta tcgaagggga attcatttct gatgaacgtg gctatgtata
     9901 cttaacagcc gtagaatcag aagaaatgac cttacaaaaa ttcaaggact tggtaaataa
     9961 caaagcgggg gagtagttaa ccctgcatct acaccaacga cagatacagg gacaccaaaa
    10021 gaaccagaac caaaaattga tacacaaggt tagccatttt ttggctagcc ttattttttt
    10081 gtaaaaacaa ggaggaaaac aaatgattga attgcaattg aaacttgacg gaaagaaaaa
    10141 aacattcaaa caacaagata tttccgcacg tgcaatgcgt gagtgtatca aattttacga
    10201 gaaagcggaa aaagcagacc taactgattt agaagcaatt gattcaatga ttgcaattac
    10261 agcagatatt ttccaagatc cagcagttac atttgatgct attttagacg gtttgactgc
    10321 gagcgagtta gtaccggcat tagaaagtgt ttttgaacaa atcaatgaac tgggaaacaa
    10381 tgaaaaaaag cagacggcga gcaaaaagag ataagttttt ctgaagctag gaaagcaatg
    10441 gatcaaatct acaaagattt aatcgaatca ggttggacga tgagagatgt ggacgaagcc
    10501 gactatcatt atttgttaca cctttttgga gaagtggaga gtggcgaaga atatgtagat
    10561 ggtgctgatt tcatcaaaca atttttatcg gctgaagact tggtaaaact tgaggaagga
    10621 ggtaaataat ggcaggaaaa ggacaaccgg caggaaatat caagctaggg attagtttag
    10681 atagcactaa ttttggtaac acgctggacg aaatcaatgc gaaagtcaaa caagctgagt
    10741 cgaatatgcg tgccaatcta aaggcttatg attcagcagg acgttcatac gaagcactta
    10801 gtcaaaagac gaaagacttg tctacggtta tggaagggca aaacgccaaa gtaagagaat
    10861 taacaaagcg ccgtgatgaa gcgattagca agtatggcga ggaatcgaaa caagttgcta
    10921 accttaacac acagataaac aatgctaccg caaaatataa tgcttacagt cgccagttga
    10981 atgacacaaa aaaagaattg gtgtattcca aaacagccgt caatgattta tctaacgaaa
    11041 tcaaagaaaa tgaacgacaa atgaacgccg aagtaaaagc gttgaaagcc gctggtgatg
    11101 aatctggtgc gtttgaagca aaacaaaaag ggctagccaa acaaacggaa ttatccgaga
    11161 aagctatcga agaacagcgc aaagttgtga aactgatggc tgatgagttt ggcgattcag
    11221 caaatgaaac cgaagatgca aaaagggcat tagaaaagtt agaacgacaa agccaaatat
    11281 ctagcaggca attagaagca ctcaaaagct ccagcgatca atcaggaaaa aaaatagaag
    11341 attttggcga caagtccaca aggtcagcta ggaaactgga tgggctaaaa gacaaattag
    11401 gctcgctaaa aagcgcattt tcgtttggtg cagttgctgg attagcgcat aacgctatta
    11461 gcagtgtagt aagtggcgtg caaggcttgg ttggcgaagc agtaaacgca tcggattcat
    11521 tgatgaagtt ttccaaaacc atggagtttg ctaactttgg gaagtcacag atagaaagct
    11581 cgaaaaaaga aatgaaagac tacgccgata agacggttta tggtttagaa gaaattctga
    11641 acacaaccgc acaattggca tctaacggga ttcctaacta tacagaacta accaaggcgg
    11701 caggtaactt gaatgccgtt gcaggcggtt ctagtgatac attcaaatcc gttgccatga
    11761 tgctaacgca gacggcagga gctgggaaac taacaactga aaactggaat caattagcag
    11821 atgcgatacc gggcgcttca gggctgttac aagacgctat gttgaaaaac ggagcttata
    11881 caggaaactt ccgtgatgca atggcgcaag gtcaaatcac ttctgatgag tttaaccaag
    11941 cgattgtaca gttaggtatg aatgacggag cagttaaagc agctacttcc acagatacat
    12001 tgagtggttc ttgggaacag atgaaatcta ctgtaataaa tgggttacaa agtatcatag
    12061 acaaagtagg cgttgaaaat atcactggtt tcatcaacag agtaacaaaa gggattgaaa
    12121 attctattcc taaaattact caatttatag gttggttgag agatattgga acgtggatcg
    12181 ttgaaaatag agagccacta acatggattg tcggaatcat aggcggaatt acattagcag
    12241 taaaagcatt gaacgtagca agtatgttgc tggcaattac tggcggaaca ttggcagccc
    12301 cttttgtggc gattggtgta gcattaggcg cactagcagg tgctttggtg atagcttata
    12361 caaaatctga aacatttaga aatatagtca atgcggcttt tacagctgtg aaaaacgtag
    12421 ttatgagcgt tatcaataat ttggtggaat actacaaaat gttgtggagc gtgttgcagt
    12481 ggctttggga aaaaataaaa gaatgggctt catggattgg caataaattc attgaaatga
    12541 agaacagcgt tgtgaatacg gttcagaacc tatggaatgg tgtgaaaaac ttcttcagta
    12601 atggcgttgg agacacttgg aataaggtag ttggctgggt aaaaaacatt ttcaacaaag
    12661 caactgaatt gaagaacaaa gtttctgatg caattggtaa cctgtggaac ggtatcaaag
    12721 acacattccg tagaggcatt gatacggtat tcaattggtt ttcagaacta ccgacgaaga
    12781 tgaagaatgc cattattggc ggtaaaaatg ccattgttga tgcgttcaaa agtattttca
    12841 acgcagcact taaagcgata ggtaaaccag ttaacgcaat catccatgga gcttcatggg
    12901 tactagaaaa actgggtgct gacaaactcg aagaatggaa agtgccacaa tacgcaaaag
    12961 gaacaccaaa cggaggtcat ccgggcgggc ctatgatggt aaatgacggt agaggtgctg
    13021 aagcggtaat cacacctaac ggacaagcat ttatcccacg agggcgaaat gtagtgttga
    13081 atgcaccaaa aggcacacac gttctaacag ctgaagaaac agcttatatg actggaaaca
    13141 aagcaccaag atatagatac gccaaaggta caggcttttt cggaaatcta tggaacaacg
    13201 tcaaaggatt tgctggagat gttggaaaca agctgaaaga tgtagtcggc gatgtatggg
    13261 attttgtaac agacccggga gcgttggcta ggaaagtgtt aaatggtctt ggcgtactgg
    13321 aagggcttgt caaatatcct ttagatgttg gtaaaggtat tctaagcaag gctaccgaag
    13381 cattgacgaa caaaatcaca gaactattca gcagtggcag tttagacact tcaatgggca
    13441 tgcaaggcgt ttacaaatac ttggcggacg ttgcagttgc agtaatgaag aagtttccag
    13501 gctttgtggc aactagtggg tatagaccag gtgaccccta ttcgcatggt aaacgtaatg
    13561 ccattgatat tgcactacct ggtgtcacag gaggctcgcc acgctacaca gaagcagcca
    13621 attacgcatt tgagaagttt gcaaacaaaa tcggctatgt tatcacaaat ggcaaggttc
    13681 gtgaccgttc aggacaatca ggtacaggta ttcacaatga ttggagacca tggcccgatg
    13741 gagatcacta tgatcatgtg catttaaacg gtgtgaaaga cccacaaaac actcaaattt
    13801 caggagatag cgtgggaggc agtggggtag aaagatggcg caatgtagca attagagcgt
    13861 tgaaaatgac cggtcaatac agtactgcaa acttaaatgc attactaaat caaatgcgta
    13921 cagagtcaaa tggtaatcct aatgcagtta acaattggga tattaacgcc aaaaatggaa
    13981 caccatcaaa agggttgctc caagtgattg acccaacatt cagacagtat gcaatgccag
    14041 gattcaacag caatattttt gacccactat ctaacatctt agcttcaatc agatacgcac
    14101 tatcaagata tggctcacta acaaatgcct atcgtggagt tggttacgca aacggtggaa
    14161 ttgtaaacca acatcaaatt gcggaaatcg cagaaggaaa caagccagaa attattattc
    14221 ctttagataa ggctaaacga tcaagagcga tgcaattatt gtctatcgca atggacaaac
    14281 taggagtttc tcctaaaact tatggaaatg cgacaactgt ttccagtgat tacgaaatcc
    14341 tagaagccat tgaaaaacag gcggcaacaa cgaatcagct gttatcgtta ttgctggcat
    14401 ttttcaaagg aaatagccga actgatagag atttagcttt agatattcag aagatcttgg
    14461 ttaggaggat gtagatgcga actgtattac tgagaaatag aataaacgaa gaaattgatt
    14521 tgtctacgga ggattatttt gctactgggt tgagtaatat ggggtttgaa gtaaaaaaag
    14581 aacatgtggg acagtggggg aatttcagag aaagcagtga gaacgttgaa atatctgaat
    14641 ttcagtcatc tgtaatcatt tctgtgcatg ggtttcgaga aaaagaactg tacaattcac
    14701 ttgtgcagtt cctatcggaa ggtccgtttg aactggaatt tgcttttgac ggcgaaacaa
    14761 tggtaagaag atgcagtcta aaatctttaa gtaagactga aatcgatccg aaaacatcgt
    14821 tactaacaga cactttagaa ctatatttca catcaaactg gtattccgta aaacgcgaaa
    14881 aattaattca gcgaccgaat gtagtgaaaa cacgtggtaa ggtttttccg tataaaagat
    14941 cttatatcta cactcaaaac ttgtgggaga aaaaaggtgt attcaaattc aataataatt
    15001 ctgtatacct aacgaatagc aaggaacgga tgtccccgct aaaaattcgt gtgattggga
    15061 agtgttcaaa tccgtattgg gaagtaatcc aaaactcaca aatcatcgca acggatggat
    15121 acttcataga tatgactgaa acacagactc tagaagtatc aagccttttt gaagatacaa
    15181 cggcgatttt aaaagatatt gcaggggtag aatcttctgt ctaccaacaa caggattata
    15241 caaaaactaa ttttgttcag gccccgactg gagaattcag cattgtgttt catgttgggg
    15301 gggcagacgt agagattgag ttatacgagg agcgtgatct gttttgattt tagcagtcac
    15361 gctttttcat cgtgatttaa tgctttataa cgaacattta ttctctaaca gttttgaatt
    15421 tggtgcggac gagatcaacg aagaggctag tagctttacg atggataagt acgttcctgt
    15481 aaaaacaggc gattttctac tagcaaaata tattcctagt ggaaaatttg catattttgg
    15541 tgtaattacg tcgcaggaag acgaaaaaat cagttgtaaa agcttactta gtttagctga
    15601 tagtgagata ccgactgcac gtgtgtcagg agataactac gaagagcata tccggcggtt
    15661 gattgaatac tatttgctga atgatccaac gaaacaacta aaagatattt tagacgtcaa
    15721 agcagaaagt gcgacctctc attcgtatca agctaccgat acgaataaac acaagttaag
    15781 tgcgtatatt ctcaatggtt tcaaaaaata caacgtaaaa tggtatttca agggaattca
    15841 aaacagaaaa atctatacag gcatacgtgc tgtaaatgaa tcaatttaca ttaaagacaa
    15901 ttcttctgaa tttagcgatt gggatgtgtt tgttcaggcg ccgggcgctg gaaacgagaa
    15961 caagctatta atcgttgata aagcaatgaa agatatagag aaaccgataa tactgtcaac
    16021 atggtatttg gacgaagaaa acaatttgac acaagatgga tcgaaagaaa atatcacgaa
    16081 accaactgtc aatttggtta atatctacga tcaaaccgca gaagataaag catcctacga
    16141 agatgtggca aaatcagagt tgaaaggcaa tacgtattcg catgaaatca aagtgaacgt
    16201 tgtaagaaat gcaaaaaatt tgaatgtcga aacgattgaa acagggatgt ttgccacgat
    16261 ttcttataaa ggaaagatat acaagtcggt tttgacagct tggcgaatat caagtgataa
    16321 ggagtttgtg gaattgactt tcggaaacat aagaagtcgt tttatggatt attttgaaga
    16381 taatggggga taaaaaatgg ttagcaatgt ggatggatat caatttgaaa acgtgaaagt
    16441 aagcgcagaa aatgatgcta gactttatca cgttttatat aatcggaaaa atcaggttat
    16501 tgatggttac gatcagtcta tgaatttatc ttcaagcgga ttaacagtaa aagttgctgc
    16561 aggagcagcg attattcaag gtcgtatggt cgttgttcga caagaagaaa gtataacagt
    16621 tccagcaaac tcaagtggtt atatagcatt aacagtggat ttgacacaag aagttatacc
    16681 tggatctatt cttccagaat cggaagaata tgaatggact aataatcaag tcaagctaga
    16741 atttataaca aaagttataa aaggtaattt aaacaatggt gataaggtct ataatttacc
    16801 actatgctca gttacttcta ctggatcaac tgtttcaatc tcaaagatat cggatagtta
    16861 cgagctgact ctctctaaag gagaaatttt gtggagtggg actgcgttaa tgcatgatac
    16921 tcaaactgtc caaccttcaa aaaaaatgtg gcagacagtt tcaggttttt tattagtatg
    16981 gctcccatac gaaaacgggc aagcaattgg ggatagatat gtaactacgc ctttttataa
    17041 agagcgtgta acttttacta atgttttagg agaaattgtt tcaggatttg atgactatca
    17101 caaaaagtgg tttagtaaac gaataaacta taattcgaat actaatatat ttacaggtgc
    17161 agcaagcaat gcaagtggag ataacgcaaa tatggtgctt aggtatatag tttcttttta
    17221 gttaggaggt atggaagagt ggcaaattta gaaattaaat tatctgcaaa taagagacag
    17281 ccttatctac gtcaccgtgt tgttggtaga gttggtgatg gggggctcac aacaatcaat
    17341 gtacaacttc ttgaggaaga tgaaattaca ccttttgtaa ttaatctaaa cggtactttg
    17401 aaatttgtgg gcgaagtttc aaacggtaac tataccgagg gagaaccaga aataatcgat
    17461 tcgactaatg ggttaattag ttacacgttc actaagtcaa atttcagcac gagtcatcaa
    17521 ttcaaacaag catattttga atatgtagat cctaacggca aaaaagtaac ttttcagaac
    17581 ttcatcatag acgtgttaga acgagtagat attaattcgg agcaagcgaa atactatatt
    17641 tcttcattgg aaaaattaca gagtgaaatg caaaccactt tcaatcagtt cattagtgat
    17701 aaacaggtcc aatacgatca aatctactcg aaatataacg aattagtaag attgataaat
    17761 gaatcagata agcaagtaaa tgatcgtatt gataagacaa atgatcgtat tgatcaaacc
    17821 aatcagcaaa tcggcgatct cggcaagctg aaaaagatgt acagtaacag catcgacttc
    17881 ggggactatg attatagtgg gaatccgaac cttagcgcta aacttaatgc ttcaagtttt
    17941 tctttaggta ctggcgcaac agtcgcggat gataatgatg agattgtgtt taccttagat
    18001 ggcacaaatc aactatcgaa gtacgctagc aaagcgcaag tgccggtagc agaagggaaa
    18061 caatatacga ttagttgtga aattatgtta gaagatgggt tcactggcga cccttcagga
    18121 atacgtctgc aacacgcata tttgccaggc ggggttgttg ttttacagac agagacagta
    18181 cctaaaaatg aattgaatac gtggcagacg cttatcggaa cgcgaacggt aaaatacacc
    18241 tctgacgtac caaatgaatg gtaccctttg tttagagaca tccgaacact taaaccgacc
    18301 ggtaaagtta gactaagaaa aattaagatt gaagaaggct caacagccac cccatatcag
    18361 ccaaacttac ttgatgaccc ttactggcta ggtaaagcgc ctttgggtga gaatattgct
    18421 aataagtctg ttacgtttcc aatcaaatct agcgcctaca gcctatacca agctaacatg
    18481 gaagaagaat ttgttttagg tcaaacctat acaattacca tgaaggcaac caaaccacct
    18541 atccaaacgt tcattgttta caacgaggat agtggagatt atagatatgg taacttagag
    18601 ccagtagaag ggttagttga tacatggggt ctgactttta caccgcagaa ggttggagtt
    18661 aactacccca aacggttaac tattatccag tatccccaat caacaacagg tgcatgtcaa
    18721 attgactggc tcaagatcga aaaaggcaat actcgaaccc cgaatattag tgagtataaa
    18781 tatcgtggta ctggtatgcg tgattcaaac aatccaaaag attatgtttg ggatctagca
    18841 ccagaatatg tcgaagataa tttggccaca gatattaaaa tttctgaaat tactggtaaa
    18901 gcaaacaatt ataccgatgg gaaagtatcg gagattaatt cgcagttgac tgcttcaatt
    18961 aatgaagtag acaccacagc taaggatgct caaacaaaag cgaatgctaa tgcgactgct
    19021 atagatgaat tagacaataa gatcgatgaa cgcattaatg atacagctac taccacatta
    19081 acagttacaa acgggaatac cggatcagca aagctttatc gtgaaggaaa aacagtttct
    19141 atatattttg tggctttaaa cggaaaaagc agtggtggaa atgattcaac gatactaaca
    19201 attccagaag gctatcggcc accaattagt tttgagcaac tggttggctc gatagaccgt
    19261 tctactttga acagtgctca gttatctatt ggtgcagatg gagccattaa atggcgaaga
    19321 aactcaagtt atggatcgga ttataccttt gcaattactt acacgattta gaaaagcgtg
    19381 aatcgatatg aaggcagcat atagaccaat tgaaccttac ggattcgagc aaatcattgt
    19441 gaatgatgaa gaacatttac cggaagaatg cacagaagtc gaaccaccga ttctaaattg
    19501 gaaaccgaaa ttcaattact gggagggaaa taaatgaaaa acatttggaa atatggacgt
    19561 actggcggag agtacgcagg aaaagtattg gacgacatgc ttgtatccgt tccttacaca
    19621 gatcagccac cgcttgaagg aattcgtgct gatggcgaac cgttaacaat cgctgatcaa
    19681 atgtttgatc ctaaattgaa tcaatggatt attttagcga acgcactaga tcacaacgat
    19741 ttaaacaatc tcaaagcaat gtatgagtcg ttagaaaatg agaacggcga tttaaaacag
    19801 atcaatgcca aactcatgct aagcgatgta gcaattaaac aggaaaatac tgcattgaaa
    19861 gaaaaagcgg atagtttagc acaaatcaat tcaaaaatga tgcttgcttc gttacaaaat
    19921 agcaaagaca tttcagaaat taaagagcaa ctaaatccag cttcaaaggg aggtgagtag
    19981 tatgtttagt tttagcgatg tgaaaatgat gtatgattgg ggctgtttta ctgacgatca
    20041 agttcgacta ttcgttccac tatgcattac agacgaagaa gcagataaaa tcattagcaa
    20101 agaagaaagc gcatcttaag tgatgcgttt ttatttaagg taaaggagtt gtcacatgat
    20161 caatttaggg gaatggggaa caatcgcagg atcaattact gcgattgttt ctttgatttt
    20221 attagtaata agaccagtca ctgcatcttt ctcgaagatt actgaaactc tttcaaaagt
    20281 aagtcacaat ttagatttgc tgactaaaga tttagaatcg agcaaatcag atcgattgat
    20341 gattcatgaa gaactaaaga aacacgatga aagattagat acacatgcag aaaaattggt
    20401 agaacacaca caacaaatta aaactttatt tagagaaagg tctcggtaaa aaagaaagga
    20461 gttaagaaga aatgatttta cccgataagt attatcaagt cattaaatgg acagttttaa
    20521 cagtattgcc agctttatct gtattagtag ccacattagg caaagcatat ggatggaatg
    20581 gaacagatat ggcagttctg actatcaatg cagcagcaac atttttaggt gttatcactg
    20641 gtgtgtcggc ttataatttg aaaaaatagg aggaaacaaa tgaaaaagaa aattactatt
    20701 actgcgatga gcctattaac ggctcttttt ttattgccga ttaatgggtt tgcctatacg
    20761 attaacaatg aatttaattt aggtgtaaac gaaggtagtt ctcaagtagc aaataatcag
    20821 tacattttac tgcatgaaac ggctaatgaa acagcaacag gacgcaatga agcgcagtat
    20881 atgcaacgtt catggactag cgcttatact gcttatattg tgggagacgg cggaattgtt
    20941 tatcaagtcg gtcaacctgg ttatgtacag tacggtgctg gttcgtatgc taatgcaaat
    21001 agtcctgtgc agattgagtt acaacacaca catgataaag caacgtttga gaaaaactac
    21061 aaggcatacg ttgaattagc tagagattca gcaatgaaat atggtattcc acttacacta
    21121 gacacaccgt ataatcaacc agggatcaaa tcgcatttat gggtaacaca aaatatttgg
    21181 ggcgatcata cagatcctta cggttatctt tctgaaatgg gcgtaagtaa agaaaaatta
    21241 gcctatgatt tggctcatgg atttaccgat gaaaatccga caacttcaga tgataaacca
    21301 gtcattgatc caactcgagc aggtgctgca aatcctaccc tgacagatgg aacaaattac
    21361 gcccacattg atcagttcgg agaaatcgaa aacgcaaact tacatgtggc tggttggcac
    21421 atcgctaact ataaatacga gtatatcttc attatggatt acaatactgg gaaagaatta
    21481 gctcgagtaa aagctgatgg aatatataga ccagatgtaa atcaagctta taatacttta
    21541 ggaaatgttg gctatcatgt atcttttaac atgcgcaact tccctaataa gaaagtctat
    21601 gtaatgatgc gggcaacgaa tgatccagaa gggaacacta aaggcggagc acaagatttt
    21661 catgataaac gctggtattt aaatattccg caacgataaa aatagcccct cgtttttgag
    21721 gggaagtaca taatgcaaat ttgtccataa cttattgatt atcttgtaaa aatgcggtaa
    21781 tttaatagtt agtgagaaga ggtgcttaat tatagttaaa aaagaggaaa tagaagcagc
    21841 atgtaaccaa gcaaatgtta ggtttattgg agaagcttta tctggatatc ctaagtataa
    21901 atacttacac aactggttac aaagtaaaag tttttatctt aataaagaaa gatgcatgcc
    21961 taataaaaat tatagggttt ataaaagagg aactattgta tatgttgatt ttggggtgaa
    22021 cgtgggttat gagttatctg gtaatcattt tgctattgtt ttaaataata aagataattc
    22081 gaaaaatggt gttgtatgtg tagtaccaat ctcttcaaaa aagaaaagta attatgtatc
    22141 tgtaggaaaa atattagaag ttgctagtat taaacaattt ataagccaag ctgataaatt
    22201 aaaagagaaa cttaggataa ttattttact ttcattaaat agaggcatca ttgatgaaag
    22261 cgatttgaat agttttatta aagatattga agctaaaggt gaaaagcttt cagtggaaga
    22321 agtagaaaaa cgagctgtaa aacataattt ggtttgcgag acaagggaac aagtaattaa
    22381 agaaataaaa tcattatctg aagatatggt aaaatatcag aaggtgttta attgctacaa
    22441 gaaatataca caagaatcct atgtaatgcc acttaatatc caaacgatta gtaaaaatag
    22501 aattagaaga attaacaagt ttgatccttc tggaaatatg aaggcacctg gaaatgtcct
    22561 caatgaagta gatgaaagtt taaaaaaata ttttacaaag taaacgatat ttttgttgac
    22621 atttttctgt tcgttagtta ccatgtagtt atagaaggac gtaagcgcct atttttaatg
    22681 tacacatcgg gacgtatgcg cccacataga aacatagttg agagacatct atatggtgtc
    22741 tcttttttta ttaatatgca aaaaagtcta gttttttcaa taatatttat gtaataataa
    22801 attagccacc acaacaaaga atgaaatcca ttattatcta gtctagtcca ttctttttgt
    22861 ttgcagtatt tgtgatggct ttccgtaccc ttagctcagt tggtcagagc agacggctca
    22921 taaccgtccg gtcgtacttc tcgtaatcca ggaaggccgt cagctccgtc cgcagtagcg
    22981 tgtttacggc cagctccaga tgggagcgga ataactcgtt caaatcacct ttggtggcta
    23041 gagtctgcag gagttctgta ataaaatcgt tcatagggaa gtcctctttt ctgtgaatgg
    23101 gtgtggtaac tttattctac agaagggact tccttttttc tatttacaca aaatatttta
    23161 tactctcaaa tgtttgtatg cagttacatc tgactttatt cgaattaagg aaaagattta
    23221 taaaaataaa ataaaaaaat atatatttaa attgacttat tgtgaaatca ttaactataa
    23281 ttagttcagt ttatgttaag gagaatgatg atatgaataa gaaaattgta gtaagtggat
    23341 taattatagg tggattagtg ttagcaggga tcgcagggaa aaactttgcc gatgacattt
    23401 tgttatgggg tggagaagat aatattgaac aaatcaatca taatttggag tcattagata
    23461 aagcactaga aaataaagag caaaatatta gtagtttaaa ttcacgatta tcttcaaata
    23521 atcaagaact tgagcaagca aaggctaacg ttgaagaata taaacaaaaa gtgttgactt
    23581 tggaaaatga taaaaatcaa ttaactatag agaaaacaaa tttagagaat caattatcag
    23641 gaaaaaatgc tgaattgcaa ggtaaacaaa atgaaatcaa tgctaaaatt gatgaaataa
    23701 atcaaaaaat agctgaaata aatcaattaa acactgattg gaataatcgt ttacaaacag
    23761 aacaaaataa atttaatgat gctcaaaata ctgttaatag tttaaatcaa caaattaatc
    23821 aattgtcaaa tgaaaaaaat actattaaaa accaattgca aagtactgaa caaaaactag
    23881 aaaaagcgca atcagaaaac agtaatttga ataattatat tgaaaaactg gaaaaagcca
    23941 aaagcgatgt agaagacaca gcaaacagat cacaagaaat cgtgactgaa catacaggaa
    24001 aatagatgca ttttttagtt taaatttctt taacaaaata gtattttgtt gctaaataat
    24061 ttttatttga atattctaca atgatatctc ccctcaaaat ggaggggaag tacatactgt
    24121 gataatctac aggtttattg actgattagg atgttattaa aaaataaaat actaaaatgc
    24181 atacccgaat ttcatacccg aaaaataaaa aatgccacca cgtacactct tttcgaaact
    24241 acctaagtag ccaagaatgt actggtgacg tgggtttggc acccaatggg ccgtgagggg
    24301 ctcgaacccg cgacccgctg attaagagtc agctgctcta ccaactgagc taacggcccg
    24361 tacaaaaagt acttaataat agtagcacta agaattttaa aatgcaagac tacatttaaa
    24421 gaaaatcaga aaaatcttta cttccttata ggaaatcatt catagattat tcacatttgc
    24481 tcgctattat ataagcatac caacaaacaa agttcttgca agagaacaaa ccctaacact
    24541 tttttcattt tttactcctt tcccgccgtc tccccggcgg gtctcctgtt cttaacgata
    24601 aaagacaaga aggcttagcc ttcttgtttt ttgttttcat ggaatcccgc taatcaaatc
    24661 acctagttaa agcaactaga ttgaatgttt atcataagtc tgatatcatg tttatgaaat
    24721 gaaaaaagga gcggttatga tggaaaaaat gcgtcagcaa ttaatggagt gggggaagga
    24781 actagaaaca ttccgtctgc ctcactggga agaactccct gacttagaac tttatatgga
    24841 ccaagtcaga acgttagttg atcgttatct ttcacctgtg atacaaggag aaaagcatcc
    24901 attgcttacc tcttctatgg taaacaatta cgttaaacta ggtttgatac cagcaccagt
    24961 taaaaaaaga tataacaaag aacacgtagc atttttactt gcaatcacta cgttgaaaca
    25021 agtattgact attcctgaaa tcaaagaagg gatattgttc caaggaaaaa cagtaggaat
    25081 tcgtgaagcc tataatctat tttgcgatga acaagaagca gctgtgtgga tggtcagcca
    25141 attagcacaa ggtaaatctc atccgcaaaa attcgaccag gcaacccctg tagaatatat
    25201 tgctgtaaga gcggcaactt tatcgtttgc aatgaaactt cttgcggaaa aaacgattgt
    25261 tctagaaacc gaatatttaa aagaggagaa aacaaatgaa aaacaataaa atcgcaattt
    25321 tagtagattc aggtacggat gtgccagaag aattgataaa aaaatacgag atgtatatga
    25381 tcccattgaa aattatttat aaagatcagg tatatacaga taaaattgac attacctctg
    25441 aagagatcta tcaacgtttg ccagaggaaa tccctggtac atctttgccg gatggtgagt
    25501 cgatcacaga aatctttgag caaatcaaaa aagacggtta tgaaaaggta cttgcggtaa
    25561 caatttccag tggattgagc gggacatata atgtggttcg tttaattgca caacagcaag
    25621 aagaattgga agcatatgta ctagatacga aaaatattgg tattggtgca ggtttttctg
    25681 cgatccaagc agcaaaatgg ctggaagaag gagtagaatg ggatcaactg atcagtaatc
    25741 tgaatgagtt agtcgaaaga acaaaagttt tctttaatgt tgcgacattg gaatatcttc
    25801 aaaaaggtgg acggatcggt ttagttgctt ctattgtagg aaccgcttta aagcttaacc
    25861 caatcatctc atgtaatgaa gaagggattt attacacagt aggaaaagct aggggacgta
    25921 aaaaaagttt ggataaaacg gttgcttacg tcaaagaaaa aatcggtaat gcaaaggtct
    25981 tcaacttagc tgttgcccat ggggatgcga aagaagaagc aattgaaatg gaagcgagac
    26041 ttaaaagaga gttcccacaa gcaaaccaaa tctactttgg ccagatttct ccggcattag
    26101 tagtccacac tggtccggga ttattgggtg tcggtgtaca agttttagaa ggataaatca
    26161 aaaaaaggaa acactgaggt gaaaatcagt gttttccttt tttattgaag ttagaacaaa
    26221 aaataataaa gtattttgca ctttcatttt aattttctct tttgttttat aggcaaagat
    26281 gaaacaaaag cagaaatacg gtataatgag aaggtatgac aataatatat aagaggtggc
    26341 tctttatgaa aaaagtttta gttgtcgatg atgagaagcc gatttcggat atcgtcaaat
    26401 tcaatttagc caaagaaggc tatgatgttt atactgcata tgatggggaa gaagccttgg
    26461 aaaaagttgc tgaggtagaa cctgatttga ttttacttga tttgatgcta cctaaaatgg
    26521 acggcttaga agttgctcgt gaggtacgca aaacttatga tatgccgatc attatggtaa
    26581 cagccaaaga ttctgaaatt gataaagtcc taggcttaga gttaggcgcc gatgattacg
    26641 tgaccaagcc attttccaac cgagaattag ttgcacgtgt caaagcaaat cttcgtcgcg
    26701 gtgccacggc tgcgaaggaa ccagaagagg cagcacctgc ggaattgacg attggtgatt
    26761 tgacgatcca tccagaagct tacatggtga cgaaacgagg agaaacaatc gagttaacac
    26821 atcgtgaatt cgaactactc ttttacttgg cgaagcacct tggacaagtg atgacaagag
    26881 aacatttatt acaaacggtt tggggttatg attattttgg agatgtacga acagtggatg
    26941 taactgtacg tcgtttgcgt gaaaagatcg aagataatcc aagccatcca aattatttag
    27001 tgacacgtcg tggtgtcggt tactatttgc gtaacccaga acaggagtaa ttggatgaaa
    27061 agaaaagtcc gcttttttcg atcagtcaat tttaagatcg ctattacctt tattctgatt
    27121 ttattgatct cgatcgaaat tattggtgcc tactttatac gtggtttaga acgttccacg
    27181 atcaatacat ttattaaaga tatgaatcaa acggtggaat cattagcaac aacaatcagc
    27241 ccggaattga atcgaaaaga taatgcagat gatgaagagg taaatgcgaa tatcaaaaga
    27301 tttattgaaa acagtgcgac tagcgatatc atcgaaatac gggtcgttga cgaaaaaggg
    27361 atcatccgag gaacaaccga tgtcaatgaa caaagcgctg tgggaaagaa aaatgattat
    27421 gttgatatca atgatttcac gactaaacgt tatgttgctt tagataacga cgataaacgg
    27481 gtgaatatca atgttcaacc gatcctgtcc ccgaccggcg atgccgttat cggggctctt
    27541 tacgtcaaaa gtaatatcga acaaaaatat tcggaaatca ataacacagc agtcattttc
    27601 tttactgcct cactaattgc ggcatttatt tcaatgatcg tatctgtttt agttgctcgt
    27661 tcgattacac agccgatcgg tgaaatgaga gaacaagcca ttcgaatcgc tagaggagac
    27721 tacagtcgga aagtcaaagt gcatggacaa gatgaacttg gacagctggc tgataccttc
    27781 aatcagttag ctgaacgtat tgaagaagct caagatacaa tggaagctga acgcaatcgt
    27841 ttggatagcg tgctatccca tatgacagac ggcgtcattg cgacagatcg caggggtaaa
    27901 gtcattacga tcaatgatat ggctgtttcc ttgctagata tcaaaaacga ggaagcaatc
    27961 ggacaatcga ttttgacact tttagatatc gaagaagaat atacgttgcg gaaattatta
    28021 gaagatccaa acgaaatggt acttgaccgt tcaaccagta cattggaaag tgatcagatg
    28081 atcttgcgag tagacttttc tatgatccgt cgcgaatcag gttttatcag cggattagta
    28141 gctgtcttgc atgatgtgac agaacaagaa aaaacagaac gggaacgtcg tgaattcgtc
    28201 tctaatgttt cccatgaact tcgtacaccg ttgacaagta tgcgtagtta tatcgaagca
    28261 ttgagcgaag gtgcgtggaa agacgaggaa gtagcaccaa attttttgaa ggtaacgcta
    28321 gatgaaacag accgaatgat tcgaatgatc aatgacctct taaatctatc tcgcatggat
    28381 acaggtaata cccagcttca attagaatat gtcaatttca acgagttagt gaattttgtt
    28441 cttgaccggt ttgatatgat ggtgggaaat caggaaaaaa attataagat ccgtcgtgaa
    28501 tttacgcaaa gagatctttg ggtcgaagta gatacagata aaatcatcca agtagtcgat
    28561 aatatcatga ataacgcgat caagtattca ccagatgggg gaacgattac ttgtcgctta
    28621 ttagaaacgc ataataacgt ggttttaagt atcaccgatc aaggattggg gattcctaaa
    28681 aaagatctga accgagtgtt cgagcggttc tatcgagtag ataaagcacg tgcaagagca
    28741 caaggtggga caggacttgg attagctatt tctagagaag tgattaaggc gcatcgaggc
    28801 gctatttggg cagagagcaa ggaaggaaaa gggtcgactt tttatatctc gctgccatat
    28861 gaaccttatg aggaggaatg gtgggaatga gaatctctga aaaagtcgtt cgtataggac
    28921 tcattttgat ggttgcgtta agcatatact tctcttatgc aatctggctg agcccagcag
    28981 gaaaaagttc aatcaacgta gatgagacaa acactcaaat gatcgagtct caaagcaatc
    29041 gaaaaacttc cgaagtcttt cttccgcttc atgttacatg gcaccattcg ggaatgatcc
    29101 aagaaaccaa cagtgaaaat ctcgtgtacc gattgcaatt gatcatcgat aaagcaagat
    29161 ttggaaagct ttctgaagta gtttcaggag acaaatcaca gtttgaacag gtgaaaaatc
    29221 tgaactctgg ttttgagttg gcctataatg cgcctttttt gctaagagaa tacaaggaag
    29281 catttgattt agatttagat gtgtcgaact tggcaaaaga aagcgaaaat atgtatttta
    29341 ctagaataca attcgatcaa ggaaataaaa aaatccgatt tttgaattat tcgaaccatc
    29401 tcgtatatga agcaaatatt tcgttgaatt ggacagatat caataaagaa ttaacagcaa
    29461 aagacacaaa atggacagaa atgacaacag aaccatcgac gatcgacacg caatatgcta
    29521 cgaaacattc gatcaaactg aaaaagtata gttatatcct gtcacagcaa ccctatacac
    29581 tttttagaaa tgcttttttc cagaaaccag atagcgtgaa aaataatgat gaaagcaatg
    29641 aattgatttt ttacggtggg aatgaaacaa tgtctatcca ttcggaaacg caagtagttg
    29701 attttcgtgg agagctgcca gagaaaaaag aaaatgctag tgtattttct gaaagtttcc
    29761 cctatattag caaattaggc agttctttag ggaacctcag atattttgac cgcagtgaca
    29821 atacgatcga ttatcggata ttcgtggaag gcttcccggt ttttgggaca gacagcagag
    29881 gacagattgg tgtggaagtt ggcggtgaaa cgaatggttc tgtgcaagtg aatatccaaa
    29941 ccagtttgaa tacgattcaa gtgccgatac cttcagacga agaagtagaa cttgcaagta
    30001 cgaatgaaat catccagcag ttaaaagcta agggagcaga tagcaacaag attggttcag
    30061 tgatcgttgg ctatacatgg caaaatgtca aagaaacgaa tcgtgtcgtt gatctcttac
    30121 ctgaatggta tgtgaaatat aaagataact ggtattcagc taacgacctt ctggaaaaat
    30181 tacctggatt ggaggcgaat taattggatt tcaaaaaaat cgaatggata tttttcgtcg
    30241 cttttttagg gttgaatgtg tttttattca gcagttatca tgacgcggta taccaagaaa
    30301 acaatgtcat tcgctctaat cagactgaat caatccaaca acgtctggct agcgacgata
    30361 tcacttacac ggggaagatt tcgtctgaga acaagcaagg ctattatctt agcgcggagc
    30421 agaccaatct tagcacttca ttagcgaatt atcgcaaggc tggtaaaagc ggcttgctgg
    30481 caagtggtgt gtttttagac ggtgatgttt tgacgaaatc tttatccagt tcagatgctg
    30541 aaaagaacgt gatcgatcca gataaaatta gtagttcctt ggaagatttt ttatctaaca
    30601 aagacaatat tttattcggt aatgattatg tgtacacgaa aaacttttct catttagaag
    30661 aagaaccact agaaatccaa gcaagccaag agtatgaagg catcccattc aatgatgata
    30721 cagcaaaaat cgttctttct ttagaaaaaa acgataatga ttatagcatc acgaaataca
    30781 tgcagacgca tttatctgat atcgagcagc tgcgcgaaaa gacggaactg tatacggaag
    30841 aggaagcaat caatacattg tacatcaata acaaaatacc aagaggatca aaaattttat
    30901 ggcgtcagct agcttattcc tgtattctga aagtccggga aaaaaatgtg tatgttcctg
    30961 tttggcatgt agcgatcgaa acaagcgata aagtggtcca aattgagagt gtcaacgctt
    31021 tcagcaatac gatcgtgaca aacaatactg taccaaaagt ggaagatcaa taaaaaagcg
    31081 agtataatga aaaaagttta caaagtaaga agggaagtag gagtcaaatg gctgaagaaa
    31141 aaccagcttt taaaatcagt atcctagcaa gtggaagttc gggtaattca ctttatatcg
    31201 aaagtgaaaa aaaacggctt ttggtcgatg caggacttag cggtaaaaaa atcacttcct
    31261 tgatggctca aattgatcgg acaccggacc aattagatgg aatacttgtg acgcatgaac
    31321 atcgagatca tatacatggc gtgggtgtct tggctcgtaa gtatcatttg gatgtttttg
    31381 ccaatcagca gacttgggaa gcgatggaac cactgattgg aaatgtacca gtggaacaaa
    31441 aacatctatt tgagatgggg aaagtcagaa cattcggcga tatcgatatt gagagtttcg
    31501 gcgtctccca tgacgcagcg gctccgcaat tttatcgttt ccataaagaa aatcggtcat
    31561 tcgtcatgtt gacggacacc ggatattgca gtgattatat gcgaggtgtc attgaaaatg
    31621 ccgatggtta tttgatggaa agtaatcatg atcttgagat gttacgcatg ggaccttatc
    31681 cgtggaattt aaaacagcgt attttaggag atagaggaca tctttccaac gaagacggtg
    31741 cacttgtcat gactgatgtg atcggtaatc ggaccaaacg aatctatctt ggccatctta
    31801 gcaaggaaaa caatatgaaa gaacttgctc atttaacaat ggaaaatgtt ttgaaagaaa
    31861 aagattttgg cgttggacat gattttgaaa tctatgatac agatccagat gaagcaactg
    31921 agctattttc gatttaaaca gttcaatcaa taaaaatata aaaagcagaa gataaaatca
    31981 atcagggttg gttttatctt ctatttttat ttttttagca tattaaaaaa ataaccgtat
    32041 tatcgactga ctacactgat gtgctgcttt tcatgtttct catccagtat ctcatctact
    32101 aatgcaatcg cgtaatctgc catgctgatc gtactgtcgc cagcagcgtt tctttgtaaa
    32161 taatcatcac ttaacaaata agtacctgta cgtttgccat gtggatcaaa taaagaagct
    32221 ggactgatat aagtccaatg tagattttta gattgtttca attttaacaa tccttcgtac
    32281 atattgaaag ctgttggata gaagggtgca tttttaggta cttggtcaat catacgagtc
    32341 gtcttttctg tatctaagaa taacgaagag gcacccccaa cgacaattaa gcgtgtatcg
    32401 gttccttcaa gcatctcaat caaatgggac aaagaagttt gatgcatttc ttcttttcct
    32461 ttgggagcgt tgaaagcatc aacgacaaca tcaaaagatg caagatcagt agttgtcaac
    32521 tcaaataaat ccttttgtat aaacggaaca tctgatggca atttttctgg atgacgcacg
    32581 attgccgtca cgtctaaatc tctggacaat gcttcgtcta aaataaatga acctgcatgg
    32641 ccagtagctc caatgagtgc aattttcata aaaaacctcc ttttttattc ctgtccattg
    32701 taagcagttg ctgaatattc gtcaaagaat ccgatttttg aaaagctcag tgcgcattcc
    32761 tttcatggtt cgtgatcatt gggtatagtt aaattaagaa aactgattga taaaaataga
    32821 agttgaagga gataagaaat gaaagagtgg ttagatcgtg cctttttacc aatgataaca
    32881 tctgttgtga atcttagacc cttaactgct ttgcgtaatg gaatgactta cacgatccca
    32941 ataattattg ttggctcgct gtttttctta tttgctcatt ttcctgtgga ttacatcgct
    33001 cagtggatag acaaaacagg attttctatg tattttacac aagtatacca ggctacattt
    33061 gatgtgatag ccttttggat cgtttttgcc attgcatacg cttatgcaaa agaagaagac
    33121 atcgatcgct taccagtcgg cataacagcc ttgtctgctt atctactgct tattcagcca
    33181 ctaaatactc agatagatac tgtctggaca ggtgctgaag ggatactagg agcagttaca
    33241 actgggctca tcatcggatg gggctatgca cgtttagtaa gacttgcgaa agggtggaag
    33301 caaagagaga agatacctga aaatgtttgg cagtctttta cttcattgat tccaatcatt
    33361 gttatagtca cttttacgct tatcggttca gcggtttttc aacagttgtt cagaatgact
    33421 gttgttcaag gagtatggac atatgtccag cttcccattc aatcgttcat agacacgttg
    33481 tttggtgtgc ttttattagg tttttttgtt ccgtttttat ggttatttgg tcttcatgga
    33541 tctgcgatgg tcaatgggtt ggttagtccc gtcttgcaag cgaattcatt agcgaatgca
    33601 gaaatcttag cctcaggaca agagttgacg gtggcaaatg gcggacatat cgttactcag
    33661 caatttctcg atcagttcat gacggttacg ggtgctggat tgacgttagg ggctgtattt
    33721 tttatgacgg tttttgcgaa atcgaagaaa tacagagaat taggaaatct tgctctttta
    33781 ccagcctttt tcaacatcaa tgaatcaatt attttttcca caccgatcgt gatgaacccg
    33841 atgatggctg ttccttttat tttcgcaccg gtcgtttcag gattgattac ctattccgct
    33901 ttatattttg gattcgttcc tttatttaca gcagtccaag tcccttggac aacaccgccg
    33961 atcctctcgg gtttcttagt aggcggagtg cctgcagcga ttttacaagg tatcgtgttg
    34021 tcaattagtt ttttcattta tttcccattt ttgaaaaaaa tagattctgc aaactgcaaa
    34081 aaggaaagac agacaaaaaa tgatggagca gcagaaaaga tgaacgacta aaaaacaaaa
    34141 atactcctgt cgctttagat tgggaaaatc accaatctag gatgacagga gtatttatag
    34201 tcatgtttat actagccctt tttggacagc taattgaaca agaacaacta atgtgttcat
    34261 tcctacgtgt gtgatcatcg atgtccagat ccgaccagtt gctttgtact ctaggctgaa
    34321 gaagaaacct aggaaaaagt agattagcag atgaccatca ttatgagcaa acgcaaacag
    34381 caaagagctg atcactgctc cgacccaaaa atttgaatat cgactgatga tccccagtat
    34441 actgcggcgg aatacgaact cttccatgat gggtccagca acagttgtcg ccaaaataaa
    34501 agcgggtgct tccatgatca tctggatgat attttgtgta ttctcggaag gggttgcttt
    34561 gccaaacaat acagattcta acatgacagc gatgctttgc agaatcaatg cgacaaagat
    34621 tccaaccaat ccataaataa caatctttgg agcagaagct ttttttaccg ctgattcgat
    34681 cgctaagggt tcacgttgct taaaataaaa aatagctagg acaacggcgc ctagtatata
    34741 catgactgca gttgctgtga tcaattcaga cgtagatttt acaagtccta tcgaagctag
    34801 gatcaagggt gagataaaaa ttagaccata gcaaaaaatg ctgaggaagc tatatttttt
    34861 taaagacatg taattcctcc ttttaaggga taacaagcat gcaaggcaag cttatatcga
    34921 ttgatttcaa ttatatcata atttcaataa gcagctgtca gaataaaatt tcttttcttt
    34981 gatttaaaag cttgcaaaaa agagggagaa tgagtattat taaaaatgta ggttagcact
    35041 cgaaggttaa gagtgctaac agattaaaaa aagagttatc ggagggattt atcgtgttaa
    35101 aaccattagg tgatcgcgtc attatcgaag tcgcaaaaga agaagaaaaa actgtcggag
    35161 gaatcgtttt agcttctgct gctaaagaga aaccccaaac tgggacagtt gttgcagtcg
    35221 gtgaaggccg tttgttagaa aacggtgaaa aagttcctgc cgctgtaaaa gcaggcgacc
    35281 aagttatgtt cgaaaaatac gccggtacag aagtaaaata cgaaggaaaa gaatatctga
    35341 tcgtcgcagg aaaagatatc atggcgattg ttgagtaaat ttggaagata aaaaaataat
    35401 caaaaataat caatgaggtg aatcaatcat ggcaaaagaa ttgaaatttg cagaagacgc
    35461 acgcgcagct atgttacgcg gggtagataa attagcagat acagtgaaag taacattagg
    35521 acctaaaggc cgaaatgtcg tactagaaaa atcttacggc tctccattga tcacaaatga
    35581 tggtgtgacg atcgcaaaag aaatcgaatt agaagatcat tttgaaaaca tgggtgcaaa
    35641 acttgtatca gaagtagcat caaaaacaaa tgatattgca ggagacggta caacgacagc
    35701 aactgttctg acacaagcaa tcgttcgcga aggtttaaaa aacgtaactg caggtgctaa
    35761 tcctctaggc attcgtcgcg ggatcgaatt ggcaacaaaa gcagcagtcg aagaattgca
    35821 taatatctca actgttgttg attcaaaaga agccattgca caagtagcag ctgtttcttc
    35881 gggttctgat aaagttggac atttgattgc tgatgcaatg gaaaaagttg gaaacgacgg
    35941 tgtcatcaca atcgaagaat caaaagggat cgaaacagaa ctagatgttg tagaaggtat
    36001 gcagtttgat cgtggttact tatcacaata tatggtaaca gataacgaca aaatggaagc
    36061 tgttctagaa aacccatata tcttgatcac agacaaaaaa atctctaaca tccaagatat
    36121 tctgccattg ttagaacaaa tcttgcaaca atcacgtcca ttgttgatta ttgctgacga
    36181 tgtagatggt gaagcattgc caactcttgt attgaacaaa atccgtggaa cattcaacgt
    36241 agtagctgta aaagctcctg gtttcggcga tcgtcgtaaa gcaatgcttg aagatatcgc
    36301 tattttgaca ggtggtacag taattactga cgatctaggt ttagaattga aagatgtaac
    36361 aatcgaaaac ttaggaaatg cttctaaagt tgttgtagat aaagacaata caacgatcgt
    36421 agaaggttct ggagaaaaag aagcaatcga agctcgtgtg caattgatca aaaaccaaat
    36481 tgctgaaaca acttctgatt ttgaccgcga aaaattacaa gaacgcttag ctaaattagc
    36541 aggtggtgta gctgttgtta aagttggtgc agctactgaa acagaattga aagaattaaa
    36601 attacgcatt gaagatgcat tgaatgctac acgtgccgca gtagaagaag gaatggtctc
    36661 aggtggtggt acagctctag tgaacgttat cagcaaagtt tctgctgtag aagcagaagg
    36721 agacgtggca acaggtatca agatcgttgt tcgtgcttta gaagaaccaa ttcgtcaaat
    36781 cgctgaaaac gctggttatg aaggatcagt gattgttgat aaattgaaga acgtggaact
    36841 aggcactggt ttcaatgctg ctactggaga atgggtaaac atggttgaag caggtatcgt
    36901 agacccaact aaagtaacac gttctgcctt gcaaaatgcc gcttccgttt ctgccttgtt
    36961 attaacgaca gaagcagtcg ttgcagataa accagaacca gcagcgccag cagctcctgc
    37021 aatggatcca tcaatgatgg gcggtatgat gtaagaaaaa gcaacttgct tatctacaac
    37081 tgatcattag tgaaagacag ataaaaagag gctggaacag aagcgtttaa ctccaagaaa
    37141 taagaagaaa ttcacggaaa ttgcttttca aatttttgtg aatttcagct tatttccgaa
    37201 ggagttgctt ctgcttccgc tgtttatacg tgtttagagc gtgagacaaa agtgttcttt
    37261 acttttgtcc cacgctcttt tttgtgtgta attataatga aaatagttat ttttaatagt
    37321 tataatattt tatctattaa aagtaaatat ccattca
//
LOCUS       DAYYME010000011        36498 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000011 DAYYME010000000
VERSION     DAYYME010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.011"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..563)
                     /locus_tag="ACHBFW_000457"
     CDS             complement(<1..563)
                     /locus_tag="ACHBFW_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316800.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHR"
     gene            complement(798..1073)
                     /locus_tag="ACHBFW_000458"
     CDS             complement(798..1073)
                     /locus_tag="ACHBFW_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFM9316801.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(1316..2626)
                     /gene="der"
                     /locus_tag="ACHBFW_000459"
     CDS             complement(1316..2626)
                     /gene="der"
                     /locus_tag="ACHBFW_000459"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFM9316802.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2808..4037)
                     /gene="rpsA"
                     /locus_tag="ACHBFW_000460"
     CDS             complement(2808..4037)
                     /gene="rpsA"
                     /locus_tag="ACHBFW_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFM9316803.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(4172..4852)
                     /gene="cmk"
                     /locus_tag="ACHBFW_000461"
     CDS             complement(4172..4852)
                     /gene="cmk"
                     /locus_tag="ACHBFW_000461"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFM9316804.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4901..5536)
                     /locus_tag="ACHBFW_000462"
     CDS             complement(4901..5536)
                     /locus_tag="ACHBFW_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFM9316805.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(5602..7005)
                     /locus_tag="ACHBFW_000463"
     CDS             complement(5602..7005)
                     /locus_tag="ACHBFW_000463"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFM9316806.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKPSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(6992..8023)
                     /locus_tag="ACHBFW_000464"
     CDS             complement(6992..8023)
                     /locus_tag="ACHBFW_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9316807.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            8054..8275
                     /locus_tag="ACHBFW_000465"
     CDS             8054..8275
                     /locus_tag="ACHBFW_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFM9316808.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(8440..9177)
                     /locus_tag="ACHBFW_000466"
     CDS             complement(8440..9177)
                     /locus_tag="ACHBFW_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFM9316809.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(9330..10670)
                     /locus_tag="ACHBFW_000467"
     CDS             complement(9330..10670)
                     /locus_tag="ACHBFW_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM9316810.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10907..12430)
                     /locus_tag="ACHBFW_000468"
     CDS             complement(10907..12430)
                     /locus_tag="ACHBFW_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM9316811.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12808..13419)
                     /locus_tag="ACHBFW_000469"
     CDS             complement(12808..13419)
                     /locus_tag="ACHBFW_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9316812.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(13542..13667)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13749..14465)
                     /locus_tag="ACHBFW_000470"
     CDS             complement(13749..14465)
                     /locus_tag="ACHBFW_000470"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM9316813.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(14471..15040)
                     /gene="scpB"
                     /locus_tag="ACHBFW_000471"
     CDS             complement(14471..15040)
                     /gene="scpB"
                     /locus_tag="ACHBFW_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFM9316814.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(15024..15794)
                     /locus_tag="ACHBFW_000472"
     CDS             complement(15024..15794)
                     /locus_tag="ACHBFW_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFM9316815.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15791..16162)
                     /locus_tag="ACHBFW_000473"
     CDS             complement(15791..16162)
                     /locus_tag="ACHBFW_000473"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9316816.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(16181..17068)
                     /gene="xerD"
                     /locus_tag="ACHBFW_000474"
     CDS             complement(16181..17068)
                     /gene="xerD"
                     /locus_tag="ACHBFW_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFM9316817.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(17164..17637)
                     /locus_tag="ACHBFW_000475"
     CDS             complement(17164..17637)
                     /locus_tag="ACHBFW_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM9316818.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(17742..18605)
                     /locus_tag="ACHBFW_000476"
     CDS             complement(17742..18605)
                     /locus_tag="ACHBFW_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFM9316819.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(18801..20900)
                     /locus_tag="ACHBFW_000477"
     CDS             complement(18801..20900)
                     /locus_tag="ACHBFW_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFM9316820.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(21120..22229)
                     /gene="rpoD"
                     /locus_tag="ACHBFW_000478"
     CDS             complement(21120..22229)
                     /gene="rpoD"
                     /locus_tag="ACHBFW_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFM9316821.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(22253..24094)
                     /gene="dnaG"
                     /locus_tag="ACHBFW_000479"
     CDS             complement(22253..24094)
                     /gene="dnaG"
                     /locus_tag="ACHBFW_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFM9316822.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(24276..26591)
                     /locus_tag="ACHBFW_000480"
     CDS             complement(24276..26591)
                     /locus_tag="ACHBFW_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9316823.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(26776..27636)
                     /locus_tag="ACHBFW_000481"
     CDS             complement(26776..27636)
                     /locus_tag="ACHBFW_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFM9316824.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(27695..28327)
                     /locus_tag="ACHBFW_000482"
                     /pseudo
     CDS             complement(27695..28327)
                     /locus_tag="ACHBFW_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            28494..29255
                     /locus_tag="ACHBFW_000483"
     CDS             28494..29255
                     /locus_tag="ACHBFW_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9316825.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            29281..31872
                     /gene="mprF"
                     /locus_tag="ACHBFW_000484"
     CDS             29281..31872
                     /gene="mprF"
                     /locus_tag="ACHBFW_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM9316826.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(32060..33247)
                     /gene="tuf"
                     /locus_tag="ACHBFW_000485"
     CDS             complement(32060..33247)
                     /gene="tuf"
                     /locus_tag="ACHBFW_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM9316827.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            33615..33971
                     /locus_tag="ACHBFW_000486"
     CDS             33615..33971
                     /locus_tag="ACHBFW_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM9316828.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(34212..35216)
                     /locus_tag="ACHBFW_000487"
     CDS             complement(34212..35216)
                     /locus_tag="ACHBFW_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFM9316829.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            35382..36233
                     /locus_tag="ACHBFW_000488"
     CDS             35382..36233
                     /locus_tag="ACHBFW_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM9316830.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            36250..36495
                     /locus_tag="ACHBFW_000489"
     CDS             36250..36495
                     /locus_tag="ACHBFW_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316831.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT        10233 a         7701 c         6297 g        12267 t
ORIGIN      
        1 aaacgatgat cacaaatcat tccgcaacag ataaaagtca aaaagtcaat ctgctttccg
       61 tccacctgcc gctcgctgat tgtttgatca taaacgatgc gttctggcag attgatggtt
      121 tgcagcacta cacgatttgg tagctgcaaa atcgtccgct gcggtgttaa tgctcggctt
      181 agagaacgga tcgatacagg gatcatccat agtgatgaat caagggttgt atgtttttga
      241 aaaaaacctg ccagatccat gatcccttca tttccaaatc cgatgataaa catggattga
      301 ttttcgggat agcgtttgac gaaatcaagc aggttcttta attctgttaa atgattgcat
      361 tgagtgttgg cacaaatata ccagtcattg tttgcttgat ttgcaaataa cgggttgatc
      421 ttatctgcaa acaaatcata atagcgctga ttcgttaaaa ataagattgg ctgatcaatg
      481 gaaagttctc ttagctgact gactagcgtt tctccgtaaa acactttcgt caacgcatct
      541 tctacttgat aatcaagttc catacgcatc cctcctcttc ttatttttat tttaccatta
      601 gtaagcaaat tctagaaaag ataagagcaa agctcacctc ataacttgca aataaataaa
      661 aattaaataa aatacatgtg ttttctatgt atgacatacg gattcggaat ctctgtctct
      721 tatttattcc ttttctgcta ggaaataaaa agaaaagcac ttgtagccct agagccacaa
      781 gcgctttttc aactgtctta tttaacagcg tcttttaatg ctttacctgg tttgaatgca
      841 ggtactttgc tagctggaat ttcgatttct tcaccagttt gtgggttacg acctttgcga
      901 gctgcgcgtt cgcgaacttc aaagttacca aaaccgatta attgaacttt ttcgccgtta
      961 gctaaagcat cttgaatagt tgaaaataca gcatctactg ctgcagttgc atctttttta
     1021 gttaaatcag tagctgaagc aactttttcg attaattctg ctttgtttgc catggataga
     1081 ttcacctcct ccacaagaga tatgatagaa atatcattta gttaaaactt tctgagtggt
     1141 ccagaagttt aaaaagaaat attgattaac ttcaatattc tgttatcacg atatcatatg
     1201 aaccgcttaa tagcaaggtt tttaccaatt ttcacatgct ttttttagtc atagaaacgt
     1261 aacaaacgaa actgtctttt tcttttgaaa aaaaaacgtc gatatggtaa aatgcctatt
     1321 ttctcctacg agggataatt cggatggacg tcccttcaaa agtaaatgct tttctgattt
     1381 gattttctaa gaaacgttca taagaaaaat gcatcaattc ctcttcattg acaaaaacga
     1441 caaatgtagg tggtttgaca gcgacttgtg tagcataaaa aatcttcagg cgttttcctt
     1501 tatctgttgg tgttggattg attgctacag catccataat gatatcattc aacacagccg
     1561 aagagatacg aagattctgg ttcatactta cttgttcgat catttcaggc agacgttcca
     1621 accgttgttt agtcactgca gaaacaaaaa cgattggagc gtaatcaaga taccggaatt
     1681 catcacgaat ctcttgttcg aaatcacgca ttgtattggt ttctttcttt acaagatccc
     1741 atttattcac gacgataata attccacgac cagcttcatg ggcgtaaccc gctactcgct
     1801 tgtcttgttc ccgaatccct tcttcagcat tcagtaccat caaaacgata tcagaacggt
     1861 caatggcacg cattgcacgc atcacgctgt atttttcagt attttcgtat acttttccgc
     1921 gtttacgcat accagctgta tctatcatta agaatttctg accactttca gaaacgaagt
     1981 gggtgtcgat tgcgtctcgg gtcgttcctt caatatccga aacaattaca cgttcttctc
     2041 ctaaaatcgc attgatcaaa gaggatttac caacatttgg gcgaccaatc aaactaaact
     2101 taatcgtgtc gtcgtcctct tcttcagtct cttcagagaa atacttcact gcttcatcta
     2161 aaatatcacc aatccctagt ccatggcttc ctgaaacagg ataaggatcg cctagcccta
     2221 aggaataaaa ttcatagata tcgtttctca tttctggatt atctacttta ttgactgcta
     2281 aaatcactgg tttattactt ctgtataaaa ttttcgcaac catctcatct gcgtctgtca
     2341 cgccttcgcg tccgctgact acacaaatga ttacatctgc ttcttcaatc gcgatttctg
     2401 cttgatgctt gatctgatcc ataaaaggtt catcgccaag atcaattccg cctgtgtcga
     2461 tcacgctgaa ttctcgacct aaccattcac cttttgcata aatacgatca cgagttactc
     2521 caggagtatc ttcgacaatc gagatacgct caccagcgat gcggttaaaa atcgtagact
     2581 taccgacgtt cgggcggcca acgattgcaa ttgttggatt tgccatatta tttcctcctc
     2641 acaagtttcc aatctcactt agtttaacga atgcattgcc aactttcaag catagattgt
     2701 caattaaacg aagtattttt attccttatt gaaatactaa tcgcttttca aaagaaaaac
     2761 tgagacaaaa gcagctgctt tcatctcagt cacagataca aaatcaatta tttttcagaa
     2821 tcatcggttg ctttttcttc tacttcttct ttcaacgcat cacctaaaat gtctcccata
     2881 gtaaatccag tattttcttc tggtaattca taagactcaa cattttttgg ttcttcttct
     2941 gaagctggtt tttcttctaa agctttgata ctcaatgcaa tacgatgttc ttctggatgg
     3001 atctctaata ctttcacttt cacttcgtca ccttcatgaa gcacttcatg tggtgtcgca
     3061 atatgtttat gtgaaatttg agagatatgg actagccctt ctactccagg gaacacttca
     3121 acaaaggcac cgaagcttgt caaacgttta actgttccgt ctaatactgt gccaactgga
     3181 gcttttgtat cgatttctga ccaaggtcca ggctgtgtgt ctttgattga caaggaaaca
     3241 cgttcttgtt caggattgat agacaacact ttgacattta catcatcatt gacagccaat
     3301 acatcacttg gttttgctac atgactatgt gaaatctctg agacatggac tagaccgtca
     3361 attccgccta ggtcgacaaa tgcgccaaaa tcagtaagac gtgcaacacg tccactgaga
     3421 acatctccct catggatagt tgaaagaatt tcttttttct tttcttctct ttcgttttcc
     3481 actaccgctt tatgcgataa gatcaaacga ttttctgatg gttcgatttc gatgatttta
     3541 aagctcaatg ttttgccttt atagtctgaa aagtctgcaa caaaatgatc ttccaccatt
     3601 gatgcaggga caaaaccacg tacaccgacg tcaacgacta aaccgccttt gacaacattt
     3661 gtaacaggtg cttcgatgac cttgccctct tggaaatctc tttcgatatc ttcccaaact
     3721 tttttagcat ctaaacggcg ttttgaaagt agatagctgc cgttttcttt atctttgccg
     3781 atcgtactga taacgactaa atctaataca tcaccgactt ttactacttc gctgatatct
     3841 tctaccggca aagttgataa ttcttttgcc ggaacgactc cttcaactcc agccccttcg
     3901 attccgacaa tcacttgttt gtcttcaatg actaaaactt cgcccttaac aacatcgcca
     3961 acttttactt cttgtacgct tttcattgcg tcttccattg actggttgtt ctcttgattt
     4021 tgatcaaatt ctgtcataac acttgtcctc ctaaccaaca ttctgccgta agtacggtac
     4081 tgtttttagt ttaattgatt attcaaaaaa aataaagcga tttcgttcgt tttccttgaa
     4141 aaaacaattg tttttcattt tttttcttaa tttaaaaaag aagataacct tttttcaaaa
     4201 caacagcttt tattgctgca acgacttctg gaatcgattt gccagtcgta tcgatacgaa
     4261 cagcatcctc tgcttgcttc agcggagaga cgtcccttgt tgaatcataa tggtctcttt
     4321 cctgtatctc tttcgtcagt gtttcaagat cagtcggaat tcctttttct tggttctctt
     4381 tgaaacgtct ttctgcacgc tcacttacgc ttgctactaa aaagattttc acttctgcat
     4441 tcggtaatac agctgtcccg atgtctcttc cgtccatgac aacgccaccg cgttccccta
     4501 tttttcgttg gacagcaact aattcttctc tgacttttcc atgtgcagag acttcagaaa
     4561 cagcttttgt gacatctggt tgacggattt ctaaagtcac atcttttgat gctatgaata
     4621 catgctgtcc atcttcttgt tgcgaaaaag agataggata ctttttgatc agcgaaacta
     4681 gcccttcttc atctgaaaaa gatacattgt tcgtaatagc taaataagtt actgcgcgat
     4741 acatggcacc ggtatctgta taaatataat tcaattcttt tgctaatatc ttggcaacgg
     4801 tacttttccc agatgaagca ggcccgtcga ttgcaatact gatttgtgtc atgatataac
     4861 tcctttttct cagaaaaaaa gccgcaaagc gcggctatta ttatttaatt cttaattctt
     4921 gaccagggta aagcataaag ttgtttggat ctaagccatt caattcaaac aattgatcag
     4981 tggtgatccc tgcacgctca gccacttgtt ttggaccttc gcctgcttga acagtcgttg
     5041 ttgaaccggc tgcagctgat gaagaaggtg ttgtttctgc tgcattgctg cttgaatccc
     5101 ctgcttgata agttgcctca cttgaggaag tcggtgttgt ctctgctgca atactgcttt
     5161 ctgacgttac acctgtatct tgagacacag tagattcttc cgcgattgta ctagattcta
     5221 ctgttgaagt ctcagttgac tcctcttttg ttgtacttgt agaactttct actactgatg
     5281 tcgaagtttc agaagaagtt gctgctgttg cattgttgct tgatccattt ctgatccaga
     5341 aataagcacc tgctggaata gcgatacata agaccagtaa aatcaaaaat agcgaaagaa
     5401 ataatgtatt tcctttcttt tgttgtctct gagaacttct tgatgaaact tcatcatctg
     5461 tatcgtagat tggttgttcc catggttctt gtgtttcgtt attttctgta gattgatgtc
     5521 tatcttttct gctcactgtt ttacctccta aactctatcg tgtgtattat accataggaa
     5581 aaagtgtctt gtcctaaaaa atcaatgttc catgtttttt ttaaataaat ttagtaaaat
     5641 cgttccccaa tgaagttgtt cagtcttaga aaaaagcttc ctctttggca gaagttctgc
     5701 ttttgctcca tcaatatcac aacaattctg cggcttttcg gagggttttt cgccaaaata
     5761 agctaagatg aattttctgc ggcagttttc ttcattaata tagcgaagca tctgctgcag
     5821 gcggaattct ttttcttttt cctgacgttt gagccgttcc acccaatttt ctggtttttc
     5881 agactgctgg atcagctcca tccatttttt ttgcagctca tcaaagggag cttgctctgt
     5941 ggcatattcc aaataaagct caaaactttg tcgctgttct ctagaaagtt gattaaagaa
     6001 ataatggata cgttcatccc cactttgata aaaaagaatt gctgcacttt cttcctgatc
     6061 tctgcctgct cgaccgattt cttgtacata gttttctaga ctgtctggca aatcataatg
     6121 gaccacataa cggatatcag atttatcgat ccccatacca aatgcattcg tagcaatgag
     6181 aaactgcagc tgatttttta caaactgctg ttgaagttgt cttctttgag ctgcatcaag
     6241 accaccgtga tagtatccaa ctgtaaaacg ttccctgaat aaatgataca atcgttcgac
     6301 ttcttttttt gttgcacaat aaatgattgc cgctccttgt gctctttcca tgaagtgttc
     6361 aagttcttgt tctttttgct gcgttttccg aacaaaaagc gagatatttt gccgattgac
     6421 tgattgtctg atctcttttg gtggtcgatc aaatagtaca tgacgtatat ctcttgctac
     6481 taaatctgtt gctgtcgccg ttagtgccag tgtgaccggg tttcctaaaa gctttcggat
     6541 ttttcctaac tgttgatatt ctggacggaa atcaactccc cattgagaca cgcaatgagc
     6601 ctcatctact acatataacg caatattttg ttttttcaga tgctctaaaa gagaaggatt
     6661 agtcaacatt tcgggactca aaaacaaaaa tttatattgc gatagatgac ggaggacgta
     6721 tcttctttca gattgcgaaa gtaaactatt gaaagcagct actcgtttct cccctcgttt
     6781 ttgtaattgc gtcacttgat cttccattag agaaagcagc ggtgacacga tgatgaccag
     6841 accttccatt aaataccccg tcaattgata gcaaaggctt tttcccgttc ctgtaggcaa
     6901 aatagcgaga gtatcttctc catcaagcaa cgcctgaata atttcttctt ggccgggacg
     6961 aaatgaatca aatccaaaat atttttttaa ttcatttatt aacattctgt atcgcttcct
     7021 ttctcaatag atagatttga tacaaacgaa attctccata atctaattgt ccatctacat
     7081 tccattcgct aaagcgccac tctcgcacgg aatccttatt tggttccaaa cgttcgattg
     7141 tttctgcaga gagaatccac tcgtatggaa aatcatcaaa aaacaaggcc cactcaataa
     7201 gatgatctgt tactgttcct ctttttaaat gacggattgc catgatctgg tcaattgtct
     7261 gaccattcat aaacatctgc ttggtgatca tcatgctttg attgaaattt tgctgtaata
     7321 atgaatcgat caaagcgtgc caaagcgaat ctggccgttt ttcgatttgt gctaacaaaa
     7381 ggtggataca tctgctttgt tgcagataaa ccgctgactc atcattgttt tttgccagct
     7441 gataaggaag cagccctgtt cgctcatttc cagataattg gttagctaaa aaatctgctg
     7501 cttcttctgg tatctccgaa aacagctgca ccaaactctc atacgcagta tcaatcaaaa
     7561 tttcacgtgg caattggctt tcactaagcc atttttttag ttggaaagta taaaaaggac
     7621 ttgtctctgc tggaagatag tcttgaatac ccgttgccag attagaaatt acttgaacag
     7681 caaatttaat gagccgccaa ctagtttccc ctgttctgcc atagcggtca aaacgcaaac
     7741 cagtatgttc tatttgatgt tcggacaaca tatcagcccc tgcaggcgtt agctttgctt
     7801 cattttcatt tatttcgatc caaccttgct gcgcaatttt ttgcaaaatt tgataaaact
     7861 tatcctgctt caattctgga aaactgttat ggataaacaa aagttcgtgg gtaaatccat
     7921 aaataagtac tgaagtcgtt cgttttccca caaggagttg gtaaagagaa ctaatccgca
     7981 gcttgtgtcc tgatgaaaat agcgataaaa taaaaacaat catattattc tccttaagtt
     8041 aggatggtta gaaatgtcac tactatgtaa acttgtacct gaacgctgta ttgcatgcgg
     8101 tctttgtcaa atcgaagcgc cagatatttt cgattatgat gaagaaggaa tcgttttatt
     8161 tgcccaagaa ccagaggctc atcaacagtt cgtgatgccg gaagaagaaa catatgtaaa
     8221 aaaagcgtat caaaaatgtc cagttcgagc gattttatta gaaaataaaa tataaatata
     8281 aacgaaactt taaaaacgta cactaagttc tctaaacttg aaaataagcc gggatctgtt
     8341 tctcttgttc cgtgaaatcc cgacttattt tgaaagattc atttgctatc tatagttaat
     8401 tgactagttt tttcacggac cctataggac ccatttatat tatttcaact ttttcagcca
     8461 gttttccaat gtattgacga tcacagtttg ctggtctgcg ttagaaattt ctgcctctcc
     8521 atctcctttt tgctcaccat agctgccaaa acctccatga tttccgccat tgattgatac
     8581 atactctgtt ctttccggta aatactgctt agcttcttga taagcttcct gattcaatac
     8641 tttatctttt gtcgccgtga tcgataaaac aggaacagct gattctgtca atgttccttt
     8701 tttgtcggga taacttgcca tgaagaatac cccttttaaa tgtttgtctg aatgattctt
     8761 attgacataa cgacttgcca ttacgccacc taatgaatgg ccaccgatca cataatcgga
     8821 atttggaaaa tcttctttga ccttatccgc tttgtttccg cccatcactg ctagatcaag
     8881 tggcattttg acaatagcga ctgaaaagcc gttttttgct aaatcttgcg cccaaatact
     8941 ataactttct ggctctacga gagcaccagg ataaaaaata agtaatggag actttccatg
     9001 attctttttc tctccttcaa aaaataagta atcttctttg ttttttcctt cttttaccaa
     9061 cacttgcgct tctgacgatg gttcgtacgt attttgctgt atatagtaat aaccggctcc
     9121 accgagtaat cctaaaatga tgactaagcc aattagtatt cgtctaaata ttttcacttt
     9181 ctcccacctt ttctatctga gtgacccctt tttcttaatc aagtatgatt ataccttaaa
     9241 aaaataagag agaaatgcaa actttccagc atcaaataaa aaaagacgga atatccattt
     9301 gaatatccgc cctttttgta tgtttatttt tatttagcac ataaacgata cacagcttct
     9361 gcataaattt ccattgcttt atacatacta cttaattccc atcgttcatt cgcctggtgc
     9421 ataaagtcag gagtatcagg gaacatcgca ccgaatgcca cacactggtt catcgtacga
     9481 gcaaaagtag cgccgcctga tacaaacggt tcagtcatgt ctccagtaat atcgcgatat
     9541 gtccccaata gatttttcac taactcactg tctagtggca cgtataatga atcaaggaag
     9601 tcaaattctt cataagtcaa cccatatttt tctgctgttt tagttaattt agctactaaa
     9661 tcatctttct tgaatgtaac agggattctc atatctacac cgatttttgt ttgttctggt
     9721 gtgatttcca aacttgcaaa gttcatcgtt agctcgccgg attgttcatc ttctgttttg
     9781 cccacgacag cttcaccagt tgcattttct tgaactaatt ttcctaagaa attgattggg
     9841 ccaaaatcaa atacttctga aagagcaatt gccagacgag aaatagcatt cactccttgt
     9901 gctgcatctt tcgcgtggat acttttacca agaacgacga tgccttcacc ttgttcttcg
     9961 aaatcgaatt cgtgtttttt caatgcttct tttacttcag aaagcttttc tccagagtaa
    10021 ggagctgcat caggaacaac attcaacgct cctccagctt ttacagaaaa ctcgttcgtt
    10081 cctggtcctg tcaagtaagc ttgcaataag cctttttctg cataaattaa aggaaattca
    10141 gcatctggcg caaatccttg cgtaattcct tcttcttttt cgttatattt ttctaaacaa
    10201 cgccataaat tttcttcatc tgtaccaaag ataaaacgaa tacgtgtctt gaattccacg
    10261 ccagcatcaa tcaaagcttt gacggcatac attgccgcaa tagaaggacc tttgtcatct
    10321 tgggatccgc gaccaactaa ccagccgtct tttatagttg gatcgaatgg ttttgtttcc
    10381 caattatttt cgtcccctgc tggaacaacg tccatatgac acaagatacc gaataattca
    10441 tctccagagc caatctctga ataaccgtaa tagccttctg gatctttaaa tgttctaaat
    10501 cctagctttt cactgatttc caatacttta tccaaagctt caatcacttt tttaccgaag
    10561 ggatgtcctt gtcctgaatc tgcttcatct agtacggaag gaattcggat caattcgctc
    10621 aatgattcta atgcttgttc atgatgttgt tctgtcacaa attttttcat gatattatcc
    10681 tcctaatatg ttaaaaaggg tcatgtcaaa ttcctccgac acaacctctt cttctcaaat
    10741 aatgacgttt taagcctgct ccatcagcaa gcagaatgtt cttaacaaat gatgaaagaa
    10801 aaaacactca ctgaatggag ctattgactt atccacgtgc ttatcgatag ccgtttatcg
    10861 gtttcttcat tcttatgata gaccttgtac ctgtatttgt ctaggtttag aacattgctg
    10921 ctacacctag gatcacacag atggctacaa ataatatcgc aattaattta ccggtaaatt
    10981 tccaccacac tgaaacatcg aaacgagcaa ttgctacagc acccattacg atagcagatg
    11041 ttggtgtaat taagtttact aaaccagatg cagattgata agcagtgatt actaaatcct
    11101 taccgacgcc ggcaaattca cccataggtc ccatgattcc cattgttgca gcagctaagc
    11161 cggaagttga aggaatcaag aatgacatag ggatatagaa aataaatgta ataatgatga
    11221 atacacttga agataatgaa cttaatcctt gttcgcccca atgtaagatg gtgtctgtga
    11281 ttaatccatt gttcataacc acttggatac cacgagcaac agctactacg attgctacac
    11341 cgatcaaatc agaagcacca gctaaaaatt cagagacaaa ttcagactct ttcatgccat
    11401 aagtgattgc tactacgatg gacattacta agaataacat agtaatttct gggaagtacc
    11461 agtcacctaa tggaagcatg cttttaccta gaaccgtacc tagtaccgga aggtttgtta
    11521 accagtttgt caatgattca aaaatcgtga aattcgcatt caaggttgtc caaggaatca
    11581 aactgatgat catgatacca aaagtaataa agaacaatac attaacatgt ttttgttttt
    11641 tcgtgattac ttcttgacga ccgattgctt caatatcgaa atgcttcata tcttctttac
    11701 gttggttgta tactaatgat tttgttggat ctttttcgat ctttgatgca taacgataca
    11761 catatacaat agaaatagct aacaaaataa cgaacatcaa gaatcgaaga ccaatacctt
    11821 cacccatcga aatcccaaca gcttgtgaag ctacgcctgt tgcaaaagga tttaccgttg
    11881 aagctagaca gcctacttga gaccctacta atacgattgc tacagctact actgtatcaa
    11941 agcctacagc catcatgact gggatcagta aagcatagaa tggaatcgtt tcttctgcca
    12001 ttccgtaagt agagccgcct aatgcaaata acagcatcaa tactggaata agcatttttt
    12061 cttttccttt gaatttcttc acaactgaag caatcccggc atctagtgcc cctgttttgt
    12121 tgataatacc taaaaatcca ccaacaacca aaatgaagaa agagattggg attgcagcag
    12181 gtgtttcttt agttagctcc cccgctggta cacctagcat cccattgatt ggcgccatga
    12241 aaatatccca cagcccttga ggattttgtt gtactcgttc atatgatcca gcgacgatgt
    12301 tgcctgcatc gtctacacta tagtgcccag ctggtataaa ccaagtaagt acagcgatta
    12361 ctgcaataat aataaataag accgtatacg ctgaaggcat ttgaaatttt ttcttttttg
    12421 tttgttccat cttcttcttc ctttcttttc cgttagagac aacaattttg gctctaatgt
    12481 tattttatca aaaagagtat cagaacaaaa ataataacac gcattttgta aaacttttgt
    12541 tcaaaaagtt cgtttttccc tattccctcc tcttttttca ggtttttaaa gctttcttct
    12601 ttttcattgt agcgtttcct tttttttata aacaaacatt ttcgtctttt aaaaggagaa
    12661 gaatttcaca tttgcaaaga attgattgat ttttatgcat aaatcaaaaa aatactcaaa
    12721 acaaacaaat aaaacgttta cattattgca tgtcttgtat ttttttccaa aaaaaaagac
    12781 caagaaattt cttgatcttt ttgatattta tttgactgct gtatgacgtt gttcgaactg
    12841 atgttgtttt cgacttaacc atggaagaag ttgcgcatgc agcactaaaa agacagcact
    12901 cacgatgaat cctttgatca agttgaaagg aacgatccca atcagtacat agtttgctaa
    12961 agacatgccc agcatctgtg ttgcgttcag ccctaaaaac attaagtaaa gaggcgtaat
    13021 aacgaaataa tttgcgatac tcataaaaat agttagagcg atggttcctg taatgacacc
    13081 taaaaatttg tttcttcctt tatgctgttt ctttacacta aagaaataat aaatcgggaa
    13141 tgtaaagatc gttgttgata agaaactagc agtgtctcct accaaattat ccggagaaaa
    13201 acctgtagtc agcagatgta atactgaacg caagaacgct gttgcaactc cggccgctgg
    13261 accgaataag aacatgctga tcaataccga gatatcacta aaatcgatct tcaaaaaact
    13321 aaaagccggc atgatcggaa aagcaaaata ctgtaaaact aatgcgatcg cagcaaacat
    13381 cgctactgca accattttct gtacacgggt gttcttcatt tgaaatcctc ctgttaggac
    13441 tcctcttcag cgaacttttt gacgtttctc tccaaatgaa aaaactctat tttccaggga
    13501 aaatagagtt agagttattt gatcaagcgt cgtcaaataa gctctatctt ctttatccag
    13561 actatactgt cggtatcgga atttcaccga ttcagctatt tttttgcaaa ataggtcgtg
    13621 gactataacc accggtcggg aatttcaccc tgcccctgaa gacgaacctt atataatttt
    13681 tgtttacata ttcattatac atatctattc aaaaaacgca acatctaaca cttataaaaa
    13741 gtaagtgttc attttgagtg gttcagcaaa ctgctgactt cttttttgtt cagttcacgg
    13801 taatccccag gacgtaggcc ttgtaaagtc agatctccgt atttttcacg ttttagtttt
    13861 tgcacaggaa gaccaactgc ttgaagcata tttttcactt gatgattacg tccttcgtgg
    13921 atcgtcaatt ccacgatact gtggtttgtc ttaggatcca ctgataaaat ttggaattta
    13981 gctggagatg tttttcgtcc ttcgatcttt attccttttg tcaaaggtgc aagcatcgat
    14041 tttgtagcga tcccttttac ctttgcaacg taaactttat ccacttcatg ttttggatgt
    14101 gtcaggcgct gagcaaaatc tccatcattt gtcagtagca agatccccga agtatcgtaa
    14161 tccagtcgac ctacaggata gatacgttct tttaccatcg gcaaaagatc cgtaacaact
    14221 tttcttcctt tgtcatctgc aacagcagaa ataactcctt taggtttgta caataaatag
    14281 taaccgtatt cttcttgata aattgggact tcatccactt ccacagtgtc atgtttccca
    14341 acttttatcc ctaattccct tacaacttgt ccgtttactt ttacacgtcc tgcaaggatg
    14401 aattcttctg cttttcgacg agaagtgatg ccagcgtggg caattacttt ctgcaatctc
    14461 tccattacaa ttattctcct ttattcattc catcaaagaa tagatctggt gacacgtctt
    14521 cttcaagtga aatttccatt tcttgtgtat caggcagttc ttctaatgat ttcaaaccaa
    14581 aataatctaa gaagtagcca gttgttccat ataatatcgg ccgacctggt ccttctactc
    14641 tacctttttc ttcaatcagt ttatgggcag ctagacgctg taccgctcct gaaacctgaa
    14701 ctcctcgaat ctgctcgatt tcagctcttg tgattggctg tttataagcg atgattgcta
    14761 aggtttcgac ggcggcttga gataatgtgt ttgatgtccc ttgcgcatat cgcttaagta
    14821 agggagccag ctctttttta gtcgttaata cataatggct ttccgtttca aaaatatgca
    14881 aagctgagtg cgagttttga gtgtaagatt cttttaattc tttcactaaa cttgtgactt
    14941 ctgcttcgct gagatttaac agtccgctta attcttcatt actgatccct tcctcgccaa
    15001 caataaaaag aagggcttca agttcactta atttcgtcat gttcggtatc tcctactgct
    15061 ggataaagca taattgattc atagttttct ttttgttgcg caatgatctg cccttttttc
    15121 atcaattcta gcaaagccat aaaagtagtg acaacttcac ttttagaagg tgactcaaaa
    15181 aaagaatcga aagtacagcc atgcgcattt ctttttatac gctcttcgat aaataccatt
    15241 cgttcttcga tcgttgtgtc atcgggcgtg atcgtttttt ccactttttg agaaaatttc
    15301 ttacgttcta agattttctg catggcacgg atcaaatcga tttttttgac ttgtcctcgc
    15361 ttcaacggct tttcagcatc cgctaaccca tcgatgttca taggttcttt tgtgtaataa
    15421 agacttcgtt cctctgcttt ttctgtcagt ctttcagctg catatttata tttacggtat
    15481 tctaaaagct gagtgaccaa ttcttctcga ggatcttctt cccataattc ttcatcgtct
    15541 gtgacgaatt cttggacagg aagcaatgtc ttgcttttga ttgccatcaa tgtagcagcc
    15601 atcactaagt attctcctgc aacagctagc tcgagtgttt tcattgtatg gatataattc
    15661 atatattgct ccgtgatcgt agcgatcggt atatcatata tatcgatttc catttgctga
    15721 atcagatgaa gcagcagatc gagaggaccc tcgaatacat ctaatttaat attgatctcc
    15781 gtcaactcat tcactctttc ttctttttct ttgctagcca ttttccaatg taaggctctg
    15841 tgatttcagt tgcacggatg gagacatccg ggtacttttc ctgtaattca tctaacattt
    15901 gaaagcccat attctcaccg cgataagacg gattcaaaga aatgtcatgg atgatcagct
    15961 gatcgttcgt tgcccagtaa ataccaataa tcccttgaat gttggttgaa ccttctggcg
    16021 taaagaaaaa aagttcataa tcgtcttcag attcgtacgt atcaatctcc ttcaacaaag
    16081 aagttttacc tgtcagtttc ttatggaaac tcaatagtcc catagcaatt ttacggtttt
    16141 ctttccggta atgtgttaac atcttctcac cttcctatga ctaagccctt ggaaaatgct
    16201 gcttataaac atccgtcatt cttttcttcg tgatatgtgt ataaatctga gtcgtagaga
    16261 tatccgcgtg acctaacaat tcctgtactg tccgcaaatc agctccattt tctagcagat
    16321 gtgtggcaaa gctatgccgc agcgtatgtg gtgttacttc tttgtaaatc ccagcctctc
    16381 gtactaactg cttcagattt ttccagattc cttgccgaga aagacctttc ccatgattat
    16441 tgacaaatac atgcatttca gatgcgtcct ttacaagtaa tggacgtgct tcgtctaagt
    16501 agcgttccag ccattggatc gcataatcac ctaaaggaat gatccgctct ttatctcctt
    16561 ttccaactgt ctgtaccagt ccaagagaaa gatgaagatc tcccaatttc aatccaatca
    16621 attcactgac gcgcatccca gtagcataca tgacttccaa gattgcacga tctcttatcc
    16681 ctaacgtttt tgtcgtatca ggtgcttcaa tcagcttttc tacttctgtc agagtcaatg
    16741 tacttggtaa tttctgtgct ttcttcggcg tatcgatatg ttgcatcgga tcatgatcag
    16801 tgatacgctc ttgccgtaaa aactgatgga atcttctgag acttgagatc atccgagtaa
    16861 cagttgctgt cgcttttttt tcttcatgca acttctccag aaaacggatg actaaaaagc
    16921 gatcgacatc ctgccatgaa gcaattttct gctcctgtag aaaatgcaag tactgttcca
    16981 aatcacgttc atagctttgt cgtgtattgg cggacaatcc ccgttcaata ttcaagtagt
    17041 ggagataatc gttgatttgc tctttcataa taatccccct catgcctctc tatcatatca
    17101 gataccgctt cattctgata gtctttttcc ataatctgag caaagaaaaa gccgtctaga
    17161 cactcagacg gactggtttc ttttttcttt ttcatagcat tcctgacaga ttccatggaa
    17221 cgttagccga tgatctttta ctaaaaaatg ataacgttct tctactactt gctccacttc
    17281 aagtaaaaga tcctcttcta cttcctcgat cgtgccgcag ttcaagcata gtaaatgatg
    17341 atggaaatgt ttggcgcctt cttttctcaa atcataacgg gctaatccat cattaaaact
    17401 tactttatcg acaattttca attctgtcaa gatttcaagg gttcgataaa cagtagcaag
    17461 tccaatttcc ggactctttt ttttcactaa aaagaagatt tcttctgcag ataaatgctc
    17521 tttctcattt tccaaaagca caagcagagt agcttctctt tgcggtgtca gcttaaaccc
    17581 cgcttcatgt agctgctgtt ttgttttttt catcgccgaa atagcattca tgaccattta
    17641 ctcaacccca acaatttata ataattacaa ataaaaaaag atataacact atgttaatta
    17701 aaattattat aagctagtta agaaaaacat gcaagggatt ctcaattaga ttttttgact
    17761 agttttgtca caccgttttc ttgtgtaatc aatccttgtt tcaaaagatt acctaatgcg
    17821 cgcttaaatg ctcctttgct gataccaaaa gctttcatga tttcttctgg atccgattta
    17881 tctgtaaagt tgatttgctg atctgcagaa cgttctaaaa tggttaaaat catttgtgca
    17941 tcatctgata tagcttcata tgctcttggc ttcaatgaaa gattcagtac accgtctggt
    18001 cggacaccaa tcactcgacc ttttatctgc tcgcctagac gaggttcatt atatcgttca
    18061 gaaggatgga taaacccaag atagaaatct tccgttaaaa cgtacgtacc tgttaatttc
    18121 aagcggtatg ctgttcctat cacatctttg tttttcattt cttctgttcc atatttgctt
    18181 aaagatttga atattttttc atctgctaaa ctgccccaaa tacggtcttt tgcatcaact
    18241 ctaagggaaa tcatcaagcg atcccctttt ttaggccata gctctgtcat tgtcggcaat
    18301 tcatcaagag atacgacaaa gtctttatct ggaagaccga tatcgacaaa cgcacctaaa
    18361 tcttttcgtg tagcagtaac gattccaaaa gcgtaatgac cgatacggct tttaggaatt
    18421 tgtgtagtga tccgattttc atgtttttga ttttggtaac cgaatccttc aactgcttca
    18481 ccgattttgt gttcaccttc tgatttgctt aagccaaagg tttgtccgtt tttttggaca
    18541 aagtagtttt tttcattttc atcaataata atacctgtaa atatagttgc taataaatcg
    18601 ttcatttttt tctccctcta tagtgaaagg ttttcttaat acatttcttt gctatcataa
    18661 cataatgtta tctacttgaa tagctgagcc ttggtttgga aaggtgaaaa aaggagtatg
    18721 acaacagttg tgtcatactc ccctttttcg aataaacggt gttaaacggc aggctcacag
    18781 cctctttcat ttgcctaaaa ttaattgctc ccatttcctc cgaaccatcc agttttacag
    18841 ttgatggtta cgatgtaaaa tggcaaagca ctttctccaa tgcctttcat caatgcagat
    18901 tttgttgaag tggcatatag attataatag ccttccccat tttcgattct ggctttttct
    18961 aatctgtaac ctccttcaac gatatatcca cgttggatac atgtagcaat cactttgctt
    19021 tggacacctg gtgcccacgt ccatgctgga ttagatgcat cggcaatatc acttgctctg
    19081 gcagatttga ttggctgatt cgctgagttt gaagaggaag gggtcgagga tgccgcttct
    19141 tgctgtgctg cttgtgcgac cgcagcttgt tgagcggctt gagctgcagc tgctgcttga
    19201 gcggcttgtt ttgcttgttc ttcagctgct tttttcgctt cttctgcttt tttcttttcc
    19261 tcttcttctt ttttctcttt atcagtgagc atcttatcta cttgtttcaa ttgttcattc
    19321 aactgagcag atagatctgt atttttcact ttttctactg cttcttttgc cgtcttgtat
    19381 tggtcgcgtg ttgctgaatc cactactttt ccgtctttaa agactatagc tgttttttct
    19441 ttagctgttt gcaattcagc atattgattt ttagcttctg tttgtgcttg ttgaatcaat
    19501 tttccaaaat ctgtgttatc cgtttcgatt gcttggatct ctttatctgc tttcaacttg
    19561 atgtcatctg ctagatgatc atcgacgatg gcaggtgcta tgaaccactc attcactttt
    19621 tggatatctc ggatcttaga ttggatatct tggtaagttg tttctaactc tgaatatcct
    19681 ttttcattct taagactgga taaatcattt tttagttttg ataaatcttg atgaatcata
    19741 tctgcacgaa taaattgatg gtgatcatct gtataaaagg cagcaagttt ctcatgaagt
    19801 tcttccattt gttgctgtct ttttgcttct gctgctactt cggcttctac tttttgatga
    19861 tggtcataat aagcccagct tcctccaccg agtagaagta ctgctgctaa aacgagataa
    19921 ggtacttttt ttgatttttt cttttgcgct ttatctggat ctgttttttc taagtttgat
    19981 tcttgatgtg ttttttcagt atctggcacg attgttattg atttcgcttc tgcttcaaac
    20041 agcttaggct ggttctcttc tttttctatt tttgcttcaa gatcagtacc ctctttgctt
    20101 acagattgat tttcagcatc ggattcacta tttatttcat cgggctctgt tgactttttg
    20161 tctgtgtttt cgagtatttc ttttttctct gcttccggca gtttattcgt tttttccttt
    20221 tctaattgtt tttcagtaaa tacttcagtt ttttctgtga tatccgtttt tacgtctttt
    20281 atttttactg tttctttacc tgtggcgttt tctctatcag agtctttttc agtttcttgt
    20341 tccttttctg gctctacttt gttttcctgt gcaatttttt cactgatcat tttgagcagc
    20401 tctttcgcgt gctcttcttt tgtatcttca cgatgtgctt tgatataagc ggatagtatc
    20461 gaattttctt cctcattatc gacgatttct ttcagttcag ctactcttct tttttgtttt
    20521 gattcaagcg tttcttcttg attctttcct gattcttccg aagtctcatg aacagttgct
    20581 ttcttctgtt catttttctc tatattagtt acttgatcgt gagcaggtgg ttcttcgtct
    20641 tctggttctg ctttttcgaa taattccatt acagattcta gaggtaaatc tttcaattcc
    20701 gaccattcga ttggatcgtt taattctgta tcaggaaaga ctgcatcttc agttgattta
    20761 tcctgcatag ctgcatctgt atttgtttct tcaattgatt cttttatttg ctgatcttct
    20821 gcagttattt gatgaccgca gtctggacag atagtattct gctccatttt tttcgatccg
    20881 caatttggac attttttcat cacttagcga tcggctcctt tactaggttt acgctaccat
    20941 ttaatctatc atattctata cgtcacgcca atagcttatt tttaggattt acttaaaaat
    21001 aatgaatgaa tttccttcct atagaaaaaa aagcttgaga ctgcgtagtc tcaagctttt
    21061 tttcttttaa aataaaccaa acagttttac tgatttctct tctggtcaat agtccacgct
    21121 tattctaaga aatcttttaa ttgttttgaa cgagaaggat ggcgcaattt tcttaatgct
    21181 ttagcctcga tctgacgaat acgttcccga gtaacaccaa atactttccc tacttcttcc
    21241 aatgttctcg tccggccatc atctaaacca aaacgtaagc gaaggacatt ctcttcccga
    21301 tcagtcaatg tatctagtac atcttccaat tgttctttta gtaactcata agctgcgtgt
    21361 tctgctggac ttgttgcttc ctggtcttct atgaaatcac ctaagtgaga gtcatcttct
    21421 tcaccgattg gcgtttcaag ggaaacaggt tcttgcgcga ttttcaagat ttctcggact
    21481 ttttctgttg gaagatccat ttccgcaccg atttcttctg gcgtaggttc tcttccaagg
    21541 tcttgcagca attgacgttg gatacgtatc aatttattaa ttgtttcaac catatgaact
    21601 ggaatacgga tcgtacgtgc ttggtcagcg attgctcgag tgatggcttg acgaatccac
    21661 catgtagcat aggttgaaaa cttaaatcct ttgcggtagt caaatttttc aactgccttc
    21721 atcagtccca tgtttccttc ttggatcaag tcaaggaact gcatgcctcg tcctacataa
    21781 cgtttagcga tacttactac taaacgaagg tttgcttctg ccagacgctg tttcgcttct
    21841 tggtcgcctt cttcgatttt caatgctaac gctacttctt cttccgcagt taaaagggag
    21901 acacggccaa tttctttcag atacatacga accgggtcat tgatttttac accggttggc
    21961 gcagaaagat cttctgtttt tgcttgttca gcttttttct cagcactctt caagctgtga
    22021 attgatggat caccattttc atccaccaca ctgatacctg cgtcttcaat cttttggatc
    22081 aatttgtcca tttcatctgc attcaaagtg aatggtgtag caagctgatt ggataaatca
    22141 tcatatacaa cttgtcgttt gggtttgttt tctttgatga aagctgctac tgctgcttca
    22201 tacgtttttt tatctgttcc atttgccata aaagaaggcc ccccttgagt agctatttgg
    22261 cttgttttaa ctgtttcgtt aaattgatga tttcaatcgt caattcaagc tcaagctgtt
    22321 tgtttccagt tcgtctggct tcttgctgca tttgtttttt taatgttatt tcatcagcta
    22381 agccagattt gctgatcaca tctagcaagt cttggatttc tcgtggcgaa ctttcttctg
    22441 ccaccggtaa atattcgatt tccaccacca accgccttaa attttcatct tgcaaaaagt
    22501 ctaaaaactc tgcaaggttg aattcataat gttgtgagac atatgcatcg aataaaaaat
    22561 aaacttctgc atatgcatca tgaataaact gaaattctcg ttcttttaaa atattacgga
    22621 cagaaacttc tttaaatgca cgatataata gaatctgctc tgccttttct acttgcgtca
    22681 aaggtctctt tttccgtgca gtttcttgtc tcgtaacctg agtgtgttga ctttcctgag
    22741 gttcttgacg attttgctga tttgtccttc gaagatcgcg cagttgtttt tgtatcgtct
    22801 ctctgccgat ctgaaactct gttgacagtt gagatagata catatcttgt tcgattggtg
    22861 aaacaactcg gctaagttca actagtaatt cttcgaggta ttcgatcttg tctttttcat
    22921 tatctaaatt tttcccttgt tttaaataac gtgatttaaa agtaaagact gtttcgcgcc
    22981 catgagccac taattcttgg aaagatgctt ctccatactt acggagatat tcatcaggat
    23041 caagcttttc tggaacgact acgacagaaa gtgctaatcg actgttttgc cgaagcaatt
    23101 caatccctcg attggttgct tcaaaacctg cgttgtctcc gtcatagcaa aagaccaatt
    23161 ctttcgtcag cttttccatt tgttggatct gttgagctgt tagacttgtc cccatagaag
    23221 ctaatccagt tttgatacca gcctgccatg cagcaatcac atccataaac ccttcgaaca
    23281 acaaaatttc tccgtttttc cggatatcgc ttcttgcttt atcaagatta aataaaactt
    23341 gtcttttatt gaacagttct gtttctggac tattcaagta tttgggctga tctttttcct
    23401 gccctttttc aggtgcaagc cagcgtcctg aaaaaccgat cgtctggcct tgagaattac
    23461 gaagtggaaa catcactctt tgataaaaac gatcagacat acggccatca tcatgcgtga
    23521 caaacaatcc agagcgttct aacaattcat ttgaaattga ttcattcttg aatacttgct
    23581 gaagaaaaga acgctcatta ggtgcaaaac ctatgttgaa ttcttcaatt aattctaaag
    23641 acaacccacg actttgcaaa tattctaaag ctggctgtcc agcttttgta tgcaatagca
    23701 tgtgatgata gatttccgct gctttttcat gagcagcaat cagtttcccc gtagcagatt
    23761 caggattttg gacagctata gtacgctgac gccattgatc ttctaacggt atctgttcga
    23821 agtctgccac ttttaccact gcttctggaa aacttagacc ttctagttct tgaagaaaag
    23881 taaacacgtt tccgccttta ccacaaccaa agcaatgaaa aatctgcttg tcttctgcaa
    23941 cagaaaaaga cggtgtacgt tcttcatgaa aaggacacag ccccaagtaa tttttaccag
    24001 actttttcaa ctggacatat tgtccaatca cctcaacgat attcgtttga ctgcgtactg
    24061 tttcgataac ctcttgagga atccgttgag ccattttttc accccctgct ctattgatat
    24121 cttttgggtt taacagaaga tatttcagga gaataaccgg taaaaaatcg cactaactga
    24181 aatcagtgcg attttaaagg acacaattat acatgtttga ttgtatcata tttcattgct
    24241 ttttcaagta taacacattt ctatctcatt aagcatcaat ttgctactct tttcagtaac
    24301 tgattttgaa tatcagcctt ccagtacaat actcgaagaa caatatttac gagcatgaat
    24361 acaaagaagt atcctacgct ctctcccaac cctaagttta cttccaaaat tccatggaac
    24421 agcattgcag ctacatatat ccctacgatc gtcgttactg ctaggaagat gcgcaatggg
    24481 ttgaagggat aacaagcttt tacaactgcc atgcaactga tcccaatcag caagtaatac
    24541 atcaaagtag tagtttcagc atttccccat gcgaatgttt ttccaagtag atacaccgca
    24601 atgatattca ctactacaag caaggcattt ggtaaagcat taataagtgc tgtaggcaaa
    24661 taacggtaag tgatttttct tttatcttgt tcaaaagata aaaagaagga aggataccct
    24721 tcaatcgcta aatcaatcag cgtgatttgg atagggataa acggaaatcc aattgctgta
    24781 acggcacata taatcgataa aataaatgaa taaatcgttt tgataaaaaa gatgcctgag
    24841 actttcgtca cattgttgac cactcggcgt ccttcaaaca ggacttctgg caatgtagta
    24901 aaatcagagt ccaacaatac cagattcgca atctgacgtg ttgctccatc accttctgcc
    24961 atagcaatgc tgcaatctgc ttctcttaac gccaaaacat cattcacacc gtcgcctgtc
    25021 attgcaaccg ttctgccgct ttctttcaat tcatttacca gcaatttctt ttgctgcgga
    25081 ctcactcgtc caaaaaccgt atagcgatga gcagcttctc gcacttccat ttcttcagta
    25141 atttgagaaa gatcgacata cgcatcataa ttcggtaatc ctgcacgtct agcgatattg
    25201 gagacagtaa aaggattgtc tccagaaatc actttcagat ccacaccttc ctcatgaaga
    25261 taagctagtg tttcttgagc gttttttcgg attggatcat cgatttcaaa cagagcaacc
    25321 ggctcaacaa atgctggttt ttcttcgttc aattgctgat ttggagcgat ccccaacata
    25381 agtacgcgat agccattcgc ttgggctgtt tttagactgt ccggaagccg atcttcttcc
    25441 accaaacgtt caggcgctcc tacgtacacg actcccagtt ctggaaattc catcgcgccc
    25501 catttgcgat cagaagaaaa tgcgactttg tttgtcacat cataccgatt agaaagctca
    25561 aaataatctc tcatagcccg catagtgatg ttgttatcac tgctttctgc cagatacgtc
    25621 cccataattt ctgggatggc tttctcgtac agcggatgta gtgcttctac ccgctggata
    25681 ctcattttcc cttcagtaat ggtccctgtt ttatccaagc ataatgtatc gacatgggcc
    25741 agcgtttcaa ttgaatacat gtcttgaaca aggattcgtt tttttgccag tttggttacc
    25801 cctgtcgtta atgcaatact tattagcaat accaaaccct ttggaagcat gcctaacaaa
    25861 gctgctgcag atgcaataac agaaattttg atagaagcat cgcggatgaa cagtgcttcc
    25921 aaaaatagta tgatccccaa aggaataatc acgaaactag tgaatttgga tactttgcga
    25981 atcgaatcta caagttcaga acgaatagct ttgtgagtct ttgcctcgct agtcaagcgg
    26041 acagcataat tatctgcacc tactcggata acttcagcca tgacagaacc gcttgtcaaa
    26101 aagcttccag aaagcaattc agctccagtt tctttgacaa tcaaatcaga ttcgcctgtc
    26161 aaaagagctt catttgcttc tgcccgtcca gatataacct gtaaatcggc tggtatctgt
    26221 tctcctgctc cgatttttac caaatctcct aataccagct ctttagtaga aatcgattgt
    26281 tctttaccgt cccggatcac tagtacttct gattgggaaa caatcgataa tttccgtaca
    26341 agatttcttg catgtatttc ttgataaatt ccaataacga tattgacgac aataattgct
    26401 aaatacgcca tgttggaata cgcacctaca aataacagac aaatcccaat accaaagttc
    26461 agaaaattga ataacgtcaa tatattatcg ctgaatattt gttttgtact ttttgatgca
    26521 tcttcctggt agtcattttg ctggccagaa gccattttct gaactacctc ttgctgtgta
    26581 agtccattca ttttcttttt ctccattttc cgccaacttt caaaaataat cacgtgttta
    26641 agtctagcgt gttccaagat taaaaacaac ttgtttttat aaaatttata gaaaaacaca
    26701 cgaatgattt tacaaaaaag atgccaacct ttttgtttga atcacccgtg tgctgttttt
    26761 attatcaatc tatctttact tatattgttc ctccaaacgg ctcatccacc aacctagtgc
    26821 tgtaccggct ataaaaagaa caggacaagc tagtacccca gcaactccca atcccgcgta
    26881 atcggtagga atgatcataa tcgttgaagc aaaaactaca cctaaaatga aatggaatag
    26941 cttgctatat gcctttgaga aaatgtaatc catgattttt gacaatccaa gaacacaaag
    27001 aatcccacca attccgatcg gcacaatcac ttcaagtcgt acttctttaa atccgtcagc
    27061 catcgcttta tacaatccca tatacaccaa aaaattagaa gggctcaatc ctgggacgat
    27121 catacccaaa ccaatcaatc cgccggcaat cagccatgtt ccaaaattct gatcgacttg
    27181 cccgtcaaat aattgagcgc cgtataataa aaacaaataa gccagaacaa aagtaacaac
    27241 catgatgatc acatcagaag tatctcttcc ttttttccct gcttctttcc ataacgcagg
    27301 aacagttcca gcgatacatc cgacaaaaaa ccataatatc gttgtttcat acgcacctaa
    27361 taaaaaactg actaaaaaag aaagtagaaa gatgcctgtc actccgccta gaccaacagg
    27421 cagaaaaaac aaaacatttt ctttgaaatt ttttgtaagg tgggctaaaa aagaaatcag
    27481 ccgctcatac atgccaaaga cagctgctaa tgcaccaccg ctgactcctg gcaagataaa
    27541 tcctgaaccg ataaacattc ccttcacaaa ccgtaacatc cagtctttgg ttgtgtaccc
    27601 cacttcttga ttttccattt tttgcctttt ttccactatt tgcgtactcc ttctttcatt
    27661 taattttatt catgattgaa catttcctgc tattttactt aaagactttt tcataggtct
    27721 ctattatttt ttcattcatt ttgacaatat caggataaaa catgttacct ccattatgat
    27781 agagatagcc gccattgtaa agcaaggact ggatccgcca atatcggtat tgtttccctt
    27841 cctcatttcc taaaagtggg ctcagcacat ctttagaata cgcttctgct aattctactg
    27901 tgttttctcc gccatgattt tttacatagg caatataatc tttcccaaaa ttatatgctt
    27961 ggacagctgt ccataaatcg catccttgtt tctttgcttc tactatcatt tccgccaaat
    28021 atttgacacc ttgatcaata ctgtcagctg gattatcaat gatctcagct tctccatgca
    28081 gactttccga gctctgcatg agatctgtcc cttttccttt tgattcggtc aatattatcg
    28141 ccttgatcaa attctcgtag ttttccatat gataattttt tgcagctgtt gttatttcta
    28201 tttgatagct ttccactcgt tttaccgaac gataagttct tcctcataaa aacaacaccg
    28261 caccaataac cgtcagaatg atcatgtaaa aaagaatacg gataatagat aatcgttttc
    28321 gcttcatttc atcacctagt tattttgaca tcttttttta tcttactatt tctttatctt
    28381 cttggctatt tcttccttga tttctgtgta cgccttaaga aaaaatgaat tttcctgtat
    28441 tttttgacat cttacgaaaa tgatcgttgt ttttcagtaa aaacaattgc ccaatgactg
    28501 atataatcgt tatactttac acagtaggtg attatgtgaa aaaagaaaag aaatatgcaa
    28561 aaatgccaga tgggagcgaa atttactatg aaaagagcgg tcaaggcttc cctctctttt
    28621 tactgcatgg caatgacggc agcggccgct ttttttcaga acaggtccct gtactcgaac
    28681 gctactatac cgtgtatcta gtagatagcc gaggacatgg tcgttcgaca aacgaagcat
    28741 ctatgttgaa ttttcagtta atggccgagg atctgaatac gatcatgttg ctggaaaaaa
    28801 tcgatcaggc agattttctt ggatttagtg atggagcaaa tctagcactg gtatttgcca
    28861 gcagcttccc taaaaaagtt catcgattga ttttaaattc tggaaacacg cttgttaaag
    28921 gcgttcgttt ttctgccaga gtcatcagta atattcatta tgcatgggta tggttactct
    28981 ctttatttcg cccaagtctt cgcaaaaatc ttttagttat caaacttttg ctgcatgaca
    29041 tcgggcttac agaaaatgat ttaaaaaaaa tcaactcccc cactttgatc atcgtgggaa
    29101 aaaaagatgt aatcaagcta aaacattctt tgtatatagc taaaactata ccaaaagctt
    29161 cctttgtgct tgtgaaagaa caaggacatg aattagctag aaaagatcct gaacgcttca
    29221 atcgagaagt tcttcaattt ctttctgaaa cataactatt aggaatagaa ggtgatttat
    29281 ttgttaaaaa ataccataca atggctgaaa aaccacctag gactgttcaa aacaatcttt
    29341 ttgatttctg tcattgtcat tattgtcggt gaattgatgt ccatcggtaa aactctatct
    29401 atccagcaat taggagaaac gtttgccact atacctgtat ggaaaagcgg actaatgcta
    29461 ttgattggtc ttatctctgt gttacctatg gttggttatg atattatttt gaatcggatg
    29521 cttgaacaaa agcagaatcc tcgttacctg tttgagacaa gctggctgat caatacgatc
    29581 aacaatattg ccggtttcgg cgggtttgtt agtgtggggc ttcgctctga actatatgga
    29641 caaaagaaag agagcagaaa cgtgatccaa gcattatcga aagttttctt gtttttgatg
    29701 gctggacttt ctatttacag tctgctttct tttttacttg tagtcttcac gccagttgct
    29761 ccttttttga agcaatattg gatatggttg atcggcggcg gtctatattt tcctgtcgtc
    29821 tttttattta ctactttcaa aaagaaagga ttccttggtg gtacctcact taaaactcaa
    29881 ggacagctat tgctagtttc attgttggaa tggaccggtg tcctgtttag tttcctttct
    29941 gttggttatt taatggaaat ccggattgat ctgtggcaaa caattccatt attcattgct
    30001 gcttcagtga taggaatcgt gtctatgatt ccaggagaaa ttgggagttt cgatgtaatg
    30061 atgattatcg ggttgtccgc tattggtgtg ccaagagaaa tagtcgttgt gtggatcttg
    30121 ctctatcgcc tgttctacta catcattccg tttttgattg gaatcgtctt tttcttcaaa
    30181 aatatcggtt ccacttttga tcagcgttat tctggtatcc ctaaacaatt agcaacagaa
    30241 attgcgcata aaatcgtggt tgtcttatta tacttttctg gaatcatgct agtactttct
    30301 gccactatcc cgcaagcttt tacagagttt cgctggcttc atagtttgaa cccattgaaa
    30361 tttcatttta ttatccaatt tcctagtata ctattgggat ttcttttaat cattatgggc
    30421 agagggatcg cagcacgtgt gaaacgagca tatttgccta caatcttctt gattgttcta
    30481 gctttacttt atgttttatt aagtgatttt agtttcacac cggtggtttt cttgtctatt
    30541 ctgcttctga tcattcttgc ttctaaaaac gaattatttc gggaacaatt gatctattca
    30601 tgggagtggc gtacgatcga tggaatcatt attggtgcac tgtccttgct ttacatcgtt
    30661 attggggtat acaatctgcc ggatttccct catcggcgtc accattttat ttcctttttc
    30721 ttatttcctt ctgaaaagat ttggttttcg ggattgttgg ccatcatcgc tgttagtttc
    30781 atgatcgttc tatttgttca tttccttcaa ggagaaaaga aacagattgg cgaagccttt
    30841 aatgaagaaa aggccctgaa gatccttaca acttatggag gaaactccga tagtcaattg
    30901 atcttcttaa aagataagcg gatgtttgcc tatgaaaaag acggtgaacc aactgttctt
    30961 ctccaattcg cttgcttcaa taataaatgt attgtaatgg gtgatccttc tggtaagaaa
    31021 gaagattttc cagaagcaat cgaagctttt atcgaagaaa cagatcgact atgctattta
    31081 ccagttttct acgaaacaag cgaagagatt gttatgattt tgcatgaatt cggctacgat
    31141 ttcattaaga tgggggaaga agcttatgta gacctgaata gttttacaac ttcagggaaa
    31201 aaaatgaagg gcactcgtgc tgtcttgaat cggatcgaac gagaaggatt tacatttgat
    31261 gtcttacaac caccattttc tactgagcaa atgagtactt tcaaaaatat ctctgataat
    31321 tggttgggtt cacgaaagga aaaagggttt tctttaggct ttttctctga agattatcta
    31381 caaagagcgc ctattgcagt tgtcaaaaat acccaagaag aaattgtcgc ttttgcaacg
    31441 atcatgccta cttacacgaa caataaagta ggaacgattg atctgatgcg atatgattct
    31501 aaaaaagcac cttctggcag tatggatttc ttgttcttgc atttgtttgc ctatatgaaa
    31561 gaagaacata tccaatggtt caatttaggg atggcccctc tttcaaatgt cggaacttcc
    31621 agaaagagtt ttttacagga aaggattgca tacttggttt acgagtttgg ttctcatttt
    31681 tattctttcc atggattaag agaatataaa agcaagtacg ctacaaactg ggtctcacgt
    31741 tatactctat attctagaga tagttggatt gcatatgtta tgattgcttt gctgattatt
    31801 gataatgcgc cagttgagca gacacaaggt agaataagtg gtttgaaaaa atggatacga
    31861 agaaagtatt aaaaactgca taccgataga tgaacactct catgatgaat aaaagagact
    31921 gaagtaaaac gaaagttcat gcttctacaa tctcttctta tacaattgaa gaacaaagag
    31981 cggtccaaac agaaatcctg tttggaccgc tcttttattt taaacatttt tcattaccaa
    32041 aatattcgag tcgcgtttct tatgcttcga tctctgtaac gatacctgaa cctactgtcc
    32101 gtccgccttc acgaattgag aatgtcgttc cgttttcaac agcgattgga tggatcaagt
    32161 caacatcaat cgtcacgttg tcaccaggca tgaccatttc tgtatcttct ggcaatgtga
    32221 ttgttcctgt aacatcagtt gtgcggaagt agaactgtgg acgatagttg ttgaagaatg
    32281 gagtatgacg tccaccttct tcttttgtca acacgtacac ttctgctttg aattttgtat
    32341 gtggagtaat ggaacctggt ttagcaagta cttgtccacg ttcgatatcg tcacgttgga
    32401 tcccccgtaa caacacgcct acgttatccc cagcttcccc gtaatctaac gttttacgga
    32461 acatttctac acctgttaca actgcttttt gtgtttcagg tttgatccct acgatctcta
    32521 cctcatcacc gacacgaaca gcaccacgat caatacgacc tgaggcaaca gttcctcgac
    32581 ctgtaattga gaagacgtct tccactggca atagtaatgg tttatctgta tcacgttctg
    32641 gtgttgggat atattcatct actgtatcca tcaattccat aatagcagct tcagcatctg
    32701 gatcgccttg caatgctttt aatgcggaac ctttgatcac aggagtgtca tcgcctggga
    32761 aaccatattc gctcaataac tcgcgaactt ccatttctac caaatcgatc aattcttcat
    32821 catcgactaa atcaactttg ttcaagaaaa caatcaggta ttttacacca acttggcgtg
    32881 acaataaaat gtgttcacgt gtttgaggca tcggaccgtc agttgcagaa acaactaaga
    32941 ttgcaccatc catttgtgct gcccctgtaa tcatgttttt cacatagtcc gcgtgtcctg
    33001 gtgcatcaat atgcgcgtag tggcgttttt ctgtttcata ttctacgtgt gcggtattga
    33061 tcgtaatccc acgttcacgt tcttctggag ctgcatcaat actagcatag tcttgaggat
    33121 ttgctaggcc ttttttgcct aatactgtag tgatcgcagc agttaaagta gttttcccat
    33181 ggtcaacgtg tccaatcgta ccaatgttaa cgtgtggttt acttctgtca taatgttctt
    33241 ttgccataat ttacaaacct cctaaataat atagatcact gaaaaatttc agctctcacc
    33301 tttttattat agtctttttt tgactaaatc aaaaagaata cgtccgactt actttttgta
    33361 agattcgaac taagcttatt atacgtcaat attggtcaaa gtcaactaag agcactcgtc
    33421 tgtcttatac gcgaaaaaag taccttttat aaataaaaaa atgtgttttt tgagaaaaac
    33481 ttcaagatag tccttaactt tcttttgaag caataagaac aaaatgagga aaagtttaaa
    33541 attcagctgt tgccaacaac tttcaataga cgaaatcttc tatttcgtta ataataagga
    33601 aagaggtgaa gtacatgaaa aaattaattg gcgtacttgt cgttttaggc atatttctcg
    33661 gcggaagttt tgcagcttat cattattttt acggcggcga agcttattat acaaaaatca
    33721 cgacgaatgg cgaaatgtca gctacccaag cagataacgg agagaaattc acgacttata
    33781 cctacaagca agcggcttat aataaagatg gtgaaaaaaa acaggtgacg cttcgcgaag
    33841 aaagagaaca tccattaaag atgaatgcgt atcttaaact caaagtcaat cctcgtaaag
    33901 gtgtattaag ctgggaagaa ataaaggctt ctgaagtgcc taaaaatgcg gcagaaaaaa
    33961 tcgataatta gtaaatggtc ttaaaatttt attagataaa aagaggctgg gacagaagcg
    34021 tttaactcca agaaataaga aggagttgct tctgcttccg ccgtttatac gtgtttagag
    34081 cgtgagacaa aagtgttctt tacttttgtc tcacgctctt tttttgttat attcgtcttt
    34141 tccgatcctt ctcttgacac aattcattta actgatcact ggtacttgaa cacctaatat
    34201 tctagtcatt tttataaacg attcaatact tcatccacat ttttgatcat cacggatatc
    34261 gtaccatccg gattattcac aaattcaatc aaatctgggt tgcgatatac atctactggt
    34321 acgatcagtt cgatcccgtt tgatagcttg aatttttgtt ttccaaattt tttctcagaa
    34381 atttcttgta cttcttttac aggaggtgct tctgggacaa atccttgctc ttgtacttct
    34441 tctttaaaag ccatttttgc cgtgatgttt tccttgaaga ctttttccgc aatcatctca
    34501 tgatccaacc gacctttttc ttctatcgta tcatagacag cttctttcaa atcagccatg
    34561 atatcaaatt cttctgtctc aaattttttt ccaagatgct tggctacttt ttggatcact
    34621 tttacatttt cttctaaaga aggtgcagga acactttcga tcactcttcc agaaaaataa
    34681 agctcttttt tgccagaaaa ctcatatttt ttttcaatta attcgtaggt ccaatcagtc
    34741 aaattcacag cgaatgcttc atctgctttt tgtgttttcg cccctagaat tgctcgattc
    34801 aaaatcaagc ggttgtcgat ccctgcttcg tctgcttcaa caaagtgagt gtaagcctca
    34861 cgataattga ctttcaataa agataagtac atcactgtat ctaattcata caagacaaca
    34921 aatacatctg cactaggtgc ttcttcactt tcttggtaga tatcgaacca ccgctgaacg
    34981 attgcttcac ttgcttccac aaaattttct tgagcgattt taagtgattc aacgatcatg
    35041 ctttcttctg ctagttgtcc agtttttgtt tgagcagaag agattttttg gatcttcttt
    35101 tgaagaaaat cacgaatata ttcttttgtc agatccagct ctttttgtga aaagactgga
    35161 gaaccagttt ctctgtcaat aatatgcaga attgcttttt ttaaatagat atccattttc
    35221 tcattccctt ctgtcttagt ttacggaaaa aaaatcaaaa ataccagaat caaaatcatt
    35281 ttcttttaaa caaaaatttc aagagtagaa acaaaatgga ttcttttttt cgaaaaatgc
    35341 gctacactta tagttgaacg aataaaaaag gagctgaaag tatgaattca ttaacaagca
    35401 cttatcgtct atcaaatggt tacgaaatcc cggtagtagg attcggcaca tggcaaacac
    35461 ctgatgggga tgtcgctgtt tcctctgtta aagaagcgct ggctgcaggt tatcgccata
    35521 tcgatactgc ccaaggatac aagaatgaag aaagcgtcgg ccaagcgatt aaggaaagtg
    35581 gaattcctag agaagagata ttcttgacaa caaaattatg gaatgcaaac cacagctatg
    35641 aacttgtgat gtcttctttt gaagaatcat tgaagaagct ccaaacagat tatttagatc
    35701 tgtttttgat ccactggcca aaccctgttg cttttcgtga taattgggaa caagcaaatg
    35761 cagatacatg gcgagcattt gaagaactgt atgaagcagg taaaatcaaa gcaatcggtg
    35821 tgagcaattt cttaccccac catctagata cactagcaaa aacagcaaaa attatgccga
    35881 tggtcaatca agttttcctt gcaccaggcg aattacagac aactgtagtt gagtatgcaa
    35941 aaaaacatga tatgatcctc gaagcatata gtccattagg cactggtaaa atcttcgatg
    36001 tgcctgaaat gaaacaaatc gctgaagcac atgacaagac gattgcacaa gttgctttgc
    36061 gttggtcgct gcaacacgaa ttccttcctc ttcctaagtc agtgacacca agcagaatca
    36121 aagaaaatac tgaattattt gattttgaat taacagaaga agaaatgaaa caaatcgatc
    36181 agttagatgg tgtagtcggc aaagcaaaag acccagatac tacccaattt taagtcgaaa
    36241 ggtgatttta tggatccaat cgaacgcttg aattctctct cggaagaagt tacccagact
    36301 tttcattccg attttgtctt tttgatcgat gcagagaaaa tccaacattt tcctgcaaga
    36361 aactggacac atgatcaaat catcgaagaa ctgaaaaaac ggttcgatca ttcgctaatg
    36421 gtcaaaccct ggcacgaaca tgaagtcatt tattcacctg aactacctgt ttttgccttg
    36481 ataccgaaaa aatagaaa
//
LOCUS       DAYYME010000012        35000 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000012 DAYYME010000000
VERSION     DAYYME010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.012"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..723)
                     /locus_tag="ACHBFW_000490"
     CDS             complement(<1..723)
                     /locus_tag="ACHBFW_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316832.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFH"
     gene            828..1073
                     /locus_tag="ACHBFW_000491"
     CDS             828..1073
                     /locus_tag="ACHBFW_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9316833.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            1229..2080
                     /locus_tag="ACHBFW_000492"
     CDS             1229..2080
                     /locus_tag="ACHBFW_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFM9316834.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            2113..2790
                     /locus_tag="ACHBFW_000493"
     CDS             2113..2790
                     /locus_tag="ACHBFW_000493"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFM9316835.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPHY
                     CIMRKIL"
     gene            3278..4243
                     /locus_tag="ACHBFW_000494"
     CDS             3278..4243
                     /locus_tag="ACHBFW_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFM9316836.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            4265..4477
                     /locus_tag="ACHBFW_000495"
     CDS             4265..4477
                     /locus_tag="ACHBFW_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM9316837.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            4552..5028
                     /locus_tag="ACHBFW_000496"
     CDS             4552..5028
                     /locus_tag="ACHBFW_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFM9316838.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            5025..6833
                     /locus_tag="ACHBFW_000497"
     CDS             5025..6833
                     /locus_tag="ACHBFW_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM9316839.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            6910..7125
                     /locus_tag="ACHBFW_000498"
     CDS             6910..7125
                     /locus_tag="ACHBFW_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFM9316840.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            7217..8080
                     /locus_tag="ACHBFW_000499"
     CDS             7217..8080
                     /locus_tag="ACHBFW_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFM9316841.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            8117..8653
                     /locus_tag="ACHBFW_000500"
     CDS             8117..8653
                     /locus_tag="ACHBFW_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFM9316842.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            8667..9089
                     /locus_tag="ACHBFW_000501"
     CDS             8667..9089
                     /locus_tag="ACHBFW_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFM9316843.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            9019..11418
                     /locus_tag="ACHBFW_000502"
     CDS             9019..11418
                     /locus_tag="ACHBFW_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFM9316844.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            11450..13441
                     /locus_tag="ACHBFW_000503"
     CDS             11450..13441
                     /locus_tag="ACHBFW_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFM9316845.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            13464..13715
                     /locus_tag="ACHBFW_000504"
     CDS             13464..13715
                     /locus_tag="ACHBFW_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFM9316846.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            13705..14934
                     /locus_tag="ACHBFW_000505"
     CDS             13705..14934
                     /locus_tag="ACHBFW_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFM9316847.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            14931..17012
                     /locus_tag="ACHBFW_000506"
     CDS             14931..17012
                     /locus_tag="ACHBFW_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM9316848.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            17161..21081
                     /locus_tag="ACHBFW_000507"
     CDS             17161..21081
                     /locus_tag="ACHBFW_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFM9316849.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            21082..22026
                     /locus_tag="ACHBFW_000508"
     CDS             21082..22026
                     /locus_tag="ACHBFW_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316850.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            22030..22233
                     /locus_tag="ACHBFW_000509"
     CDS             22030..22233
                     /locus_tag="ACHBFW_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFM9316851.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(22533..22769)
                     /locus_tag="ACHBFW_000510"
     CDS             complement(22533..22769)
                     /locus_tag="ACHBFW_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9316852.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(22952..23323)
                     /locus_tag="ACHBFW_000511"
     CDS             complement(22952..23323)
                     /locus_tag="ACHBFW_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFM9316853.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(23320..24648)
                     /locus_tag="ACHBFW_000512"
     CDS             complement(23320..24648)
                     /locus_tag="ACHBFW_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM9316854.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(24609..24938)
                     /gene="mobC"
                     /locus_tag="ACHBFW_000513"
     CDS             complement(24609..24938)
                     /gene="mobC"
                     /locus_tag="ACHBFW_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM9316855.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(25196..25566)
                     /locus_tag="ACHBFW_000514"
                     /pseudo
     CDS             complement(25196..25566)
                     /locus_tag="ACHBFW_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            25711..25896
                     /locus_tag="ACHBFW_000515"
     CDS             25711..25896
                     /locus_tag="ACHBFW_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM9316856.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            26054..26221
                     /locus_tag="ACHBFW_000516"
     CDS             26054..26221
                     /locus_tag="ACHBFW_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316857.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            26839..27498
                     /gene="vanR-B"
                     /locus_tag="ACHBFW_000517"
     CDS             26839..27498
                     /gene="vanR-B"
                     /locus_tag="ACHBFW_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM9316858.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            27501..28862
                     /gene="vanS-B"
                     /locus_tag="ACHBFW_000518"
     CDS             27501..28862
                     /gene="vanS-B"
                     /locus_tag="ACHBFW_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM9316859.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(28953..30254)
                     /locus_tag="ACHBFW_000519"
     CDS             complement(28953..30254)
                     /locus_tag="ACHBFW_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9316860.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            30533..31339
                     /locus_tag="ACHBFW_000520"
     CDS             30533..31339
                     /locus_tag="ACHBFW_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFM9316861.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            31357..32064
                     /locus_tag="ACHBFW_000521"
     CDS             31357..32064
                     /locus_tag="ACHBFW_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFM9316862.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            32061..33032
                     /locus_tag="ACHBFW_000522"
     CDS             32061..33032
                     /locus_tag="ACHBFW_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM9316863.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            33025..34053
                     /gene="vanB"
                     /locus_tag="ACHBFW_000523"
     CDS             33025..34053
                     /gene="vanB"
                     /locus_tag="ACHBFW_000523"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM9316864.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            34059..34667
                     /locus_tag="ACHBFW_000524"
     CDS             34059..34667
                     /locus_tag="ACHBFW_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM9316865.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(34733..34906)
                     /locus_tag="ACHBFW_000525"
     CDS             complement(34733..34906)
                     /locus_tag="ACHBFW_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316866.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         9179 a         8584 c         9164 g         8073 t
ORIGIN      
        1 atgaaatccc gtatttatag tgctcaattg atcaactaac catgttttac tttttgaatt
       61 gtagataaat ctaaaattaa tatcgttatt atcgtgttga aggggcgtct ccaaataatc
      121 tgctgtatat tcatagtcaa cgtaacctgc taatacaaaa gagagtatcc ctttatttaa
      181 tgttagattt aatggataat catacgactt tatttttttc aacttcatat tgtacatatc
      241 ttcagccata aaagttgagt tcgtgatttc atcttgggtc tcattaaaaa agttatccga
      301 ataatcgctt atttttgaaa agtctttatt ggcaccagcc tcaaaaaatt gttcaacttt
      361 ttcagtataa agttgtgcta acgctccttt ttcaatatta actttaatat tttcatgtct
      421 atcaatttgt gtgctgttca gtaagggaag atcagctttt agttttatat cgtcttgatt
      481 tctttcaatt tcaattggca aaatcttttc agtagattca gtttctgata aaggaacatc
      541 tagtttttta tcatgataat aagcttcaaa tccatctgga accggaaaaa aatgtattgg
      601 gtaattcttt tctgaggttg ttgtactagt ttccgtactt gtagaacttg tgtcagaagt
      661 ggtacacccg cttatagttc cgatgagtaa agcgcaagtt agaatgattg caaatttctt
      721 caaaaaaatt cctccttaaa attgaatatg gactaatact agtcccattg tagcataccg
      781 aatctttaag attaataaga aaaaggacta attttagtcc aaaaggtgtg ctattcttga
      841 aagaggaaaa aattattgat aaagaaaatg taatcggtag caatattaga cgagtccgat
      901 tagaaaaagg aattggtcaa accgagctga ttagagacct acagcttcgt aaaattgcta
      961 taactaggga aacgttggtt aagattgaag gtggccgcca acacattaag ttggatcaat
     1021 tgaaagccat caaagatatt ctacaggtgt cttatgaaga tttgcttcaa tgactacttg
     1081 tagtcatttc gtttgaaatt attatgttac acccttatgt gtcacgaaga aatactggta
     1141 taataatgaa tgggatatgt aacgataggg tatactgtta tgttgcctaa taaattgtgg
     1201 atcaaaatac aagaatggag ggatatcctt gtataaggca tatgaattgg attttacaaa
     1261 tgctgactta aatgcattct cctcaagctc gcaatcatat acacatgtag taaatcaaat
     1321 agatcacaac caaaaatcta ttaacaagag aatagaaaat ctatttgaaa gtgaaaatga
     1381 tttagttgat tattattcta aggacagcaa aattctagat gcagacgaaa ttattaaaga
     1441 ttggtttcca actataaaag cagatatttt tatatctcac tctcatgctg atgaaaaatt
     1501 agctatacgt tttgcatcat ggctatttga aaattttggt cttactgcat ttattgattc
     1561 ttcggtttgg ggatacagtt ctgatttatt aaaaaaaata gaccaaaaat attgctataa
     1621 ggaacaaaca aaaacatatg attatgataa aagaaatgta acaactagtc atgttcatat
     1681 gatgctttct acagctttaa ataatatgat tgatagtaca gaatgtctgt tttttcttaa
     1741 tacacctaat tcaattagtt tgagcaatga aatcactaat gaacagaaat ttacgtattc
     1801 gccatggcta tatagcgaac tgacgacagc ttcaatagtg gaaaaaaaga atccacgatt
     1861 ggaaagtaat cctcaaatga gcaccgagga tgttagaagc attataaagc actattcttc
     1921 gcaagaaaat ctgcaaataa aatattcttt aaatttaagt gaattaactt atttacgaaa
     1981 agctgatttg aaaaaatggg ctttagatgc tgtggaatgc actaaaggaa gtaatacatt
     2041 agatatttta tataaaaaca ccattttcac taatctctaa tcaatttact gaatcgaaag
     2101 gaaccgggta gaatggataa gaaagtttta ataaagaggt tctctgaagc tattgaaaaa
     2161 ggaaatgcag caatatttgc aggtgcagga ttatcgatgt cacaaggata tgtaagttgg
     2221 ccggaactac ttaatgatcc tgcaacagaa atcgggttgg attctaaaaa agaaacagac
     2281 ttagttactc tggctcaata ttataaaaat gaaaatggtg gatcaagagg aattttgaat
     2341 cagatactta tggataattt tggtgaagaa ttagaaatat ctgaaaatca tcgaatacta
     2401 gcaagtttac ctattgaaac ttattggaca actaattatg atcatctaat agaaaaatca
     2461 atccgagaag cttataaaaa ccctcaggtt aagaaaaatt atactcagtt agcaacaact
     2521 aatccaaatg tggatacaat tgtttataaa atgcatggtg atatagatga tgtcagttcg
     2581 acagtaataa caagagatga ttatgaaaaa tatgatgatt cgtacgcgtt atttaaagaa
     2641 actttaaaag gagatttgtt aacaaaaact tttctctttt taggatttag ttttacagac
     2701 cctaatttag aaagaatttt atcagatata agatgggttt tgagagaaaa ccaaagacct
     2761 cattattgta taatgagaaa aatattatag aaaaaatttt aggttatcgc tgaaagcccc
     2821 ggaaatacgg gcatttcgca ggatatgaga actcaattta ctaccaattt actactttcg
     2881 gattgagccg catatatgct gaaccatact gctaataccc gccaccccaa atacggcaca
     2941 tcataaattg aatacgacac cgtaaacgcg gcgttcctct ttcccgtcat gggagaacag
     3001 gacgccgctt ttttatgccc aaatagagaa aggaggcgac gccatgctat ttacttccgg
     3061 ccctgaccgg gcgtttgaac ggctgatgca ggaaaagccc ggtttcgatc actacgacgg
     3121 cggcggggcg gacgcgccgg aggattgcgg cacctgccga ttttaccgcc cggattggaa
     3181 atatcaattc tgccagtttg cagagtgtcc ctatcagccg ggcaagctga ccgactatgc
     3241 agcggtcaaa ttcacagtga aaggagcgaa caacgctatg gcagttttcc gtgtggaaaa
     3301 gaacaaaggc tatacggtga tgtcaaatca ccacctgcgg aataagaacc tgaccttaaa
     3361 ggccaagggc ctgctgtcgc agatgttgtc cttgccggag gattgggact ataccctaaa
     3421 aggactgtcc tatatcaacc gggaaagcat cgacgcgatc aggacagcgg tatgggagct
     3481 tgaaaaagct ggatatatcc ggcgtgagca gggccgtgac gcaaaaggca aaatggccga
     3541 tatggtctat accatctacg aacagccggt attggaaaac ccggtattgg aaaatccaac
     3601 atcggataac ccggtgttgg aaaatccgac atcggataat ccggcgtcgg aaaatccaac
     3661 gcaattaaat aaagaagtac aaagaactga cttatcaaaa aaagaaaaaa taattacaga
     3721 tggacaaagt acccattcca ttcctatccc atccccgacc cctgccccta tggagggcga
     3781 ggcagcggct acgccgccgg aacggaaaag gacaggaaag gatgaggctg tacggattta
     3841 tcgggaaatc attttggaga acatcgagta tgcctatctc attcaggaca ggagcattga
     3901 ccgtgaacag cttgacgaaa tcgtagacct gatgctggaa accgtctgca ccgcccgaaa
     3961 gacaatccgt gtcgccgggg acgactaccc tgccgagctg gtgaaatcca agtacatgaa
     4021 gctgaccggc gaacatatcc gctttgtcct tgactgtctg cgggagaaca caacgaaagt
     4081 ccgcaatatc aaacaatacc tgcgggctat gctgttcaac gcccccagca ccatttccaa
     4141 ctactacacg gcccttgtcg cgcacgacat ggcacagccc gattggggaa agcctaaatc
     4201 cggcctgccg gactattcct gcggcgaggg cgaaagcctg taacgagaaa aggaggacga
     4261 ccctatgaag cagggcgcat tgatttttga cgagcgaaca gaccgttacg acatccgctt
     4321 tgatctggcc gactattacg gcggcctgca ttgcggggag tgtttcgacg tgatgatcgg
     4381 cggcaaatgg aggcccaccc gcattgaaat ggccgaaaac tggtatcttg tcggcatccg
     4441 ggccgacgac ctttccggcc tgcgggtgcg gatttgacct atatctacct gcggcgtctg
     4501 gtggttccgc cttagacgcc gcttttgact ttctgacaag gagggatgcg attgcaggaa
     4561 gaagttactc aaaagaccat tgccctgtcc atgaaaacgg gcaagctcac ggctcaagtg
     4621 ctgcaagcgg cgctgaaaaa gtacctgcaa caccgggcca agggcaaaaa cacgctgcac
     4681 cacggccagc agagcctaaa gcagcttaaa aagcatgggg ctgatctctc caacattgag
     4741 atcaccgagg ccaacattgg agcgttcaag ccatgcgcca agaaatacgg cgtggatttt
     4801 accttacgaa aagataagac cacccagccg ccccactacg ttgtgatttt caaggccaag
     4861 gatgcagata accttgatct ggcgtttcgg gagtttaccg ccaagacgct ttccaaggag
     4921 cagcggccct ctatccgcaa ggttttgtcc gctgtgaagc aaaaggcggc ggcccagacc
     4981 aagcagcggg ccaaggagaa aatcaaggaa agggggctgg aactgtgaag cctgacgtaa
     5041 agaaattgct gatcctgaac ctcccgtatc tgatttttgt gtacctgttt gcgaaatgcg
     5101 gggaggcgta ccggctggcc gctggtgctg acctgtcggg gaagctcctg cattttgcgg
     5161 atggttttgc ggcggctttt gccaaccctc tgccaagcct gcacctgttt gacctgtgta
     5221 tcggtatcgc cggtgccgtg atcgtgcggc tggtggtgta ctgcaagagc aagaacgtga
     5281 agaaataccg caaaggcatg gagtatggca gcgcccgctg ggggacggcc aaagacatag
     5341 cgccttacat cgaccccaag tttgaaaata atatcctgct cacccaaacg gaacggctta
     5401 ccatgaccgg caggcccaaa gaccccaaga cagcgcggaa taaaaacgtg ctggtgatcg
     5461 gtggcagcgg ttcagggaaa acgagatttt tcgtgaaacc caacctgatg caatgctttc
     5521 cgaccacgga ctatccgact tcgtttgtgg tgacagatcc gaaaggcact ttggttctgg
     5581 aaaccggcag gatgttccag caggcgggct accgcattaa aatcctgaat accattaact
     5641 tttccaagtc catgaagtac aacccctttg tctacatcca ttcggaaaag gacgtgctaa
     5701 agctggtaaa taccctgatt tccaacacca agggcgaggg ggaaaagtca gcggaggatt
     5761 tttgggtgaa gtcggaacgg ttgttttatt cggccctgat cggctatatc tggtacgagg
     5821 ccccagcgga ggaaatgaac ttctcaacgc tgctggaaat gataaacgcc agtgaagccc
     5881 gcgaggacga cccggagttt caaagccccg tcgatctcat gtttgagcgt ttggagcaga
     5941 aagaccccga ccattttgcg gtgcgccagt acaagaaatt cctgctgtcc gcaggcaaga
     6001 cccgttcttc catcctgatt tcatgcggtg ccagacttgc gccctttgac atccgggagg
     6061 tacgcgagct gatggaggac gacgaaatgg agctggacac catcggggac gaaaagacgg
     6121 tgctgtatct cattatgagc gatacggaca cgacctttaa tttcattctg gctatgctcc
     6181 aaagccagct aatcaacctg ctttgtgacc gggctgatga tgtgtacggc gggcggctgc
     6241 ctgtccatgt acgcctgatt ttggatgaat ttgccaacat tggccaaata cccaatttcg
     6301 ataaactcat tgccaccatt cgaagccgtg aaatatcggc ctctattatt ttgcagagcc
     6361 agtcgcagct aaaagcgatt tacaaggacg ccgccgagat catttcggat aactgcgata
     6421 gtgtgctttt ccttagtggg cgcggcaaaa atgccaagga gatttccgat gcgctgggga
     6481 aagaaacgat tgacagtttc aacaccagcg agaaccgggg aagccaaacg tcccacggat
     6541 tgaactatca aaaattagga aaggagttga tgtcccaaga cgaaattgcc gtgatggacg
     6601 gcggcaagtg tatcttgcag ctacggggtg tgcgcccgtt tttatcggag aaattcgata
     6661 ttaccaagca tccccggtac aaataccttg ccgacgccga caagaagaat acctttgacg
     6721 tggacagatt tttgactatc acgcgccgga aacggcagca agtggtaacg caggatgaag
     6781 tattcgacct gtatgaaatc gacctgtcgg acgaggacgc cgccgctgaa taaacagcgg
     6841 cttttttcat tccaacaaca aaatcaaaaa atcattatga gccggaaacc ggcagaaaga
     6901 ggtaaattta tggcattttt taattccgca gttactactt tgcagactat cgtgatcgga
     6961 ctgggcggcg ctctgtgcgt atggggcggc atcaatctgt tggagggata cgggcaggac
     7021 aatccggggg caaaatcgca gggtgtcaag cagcttgtcg ctggcggcgg cgtggccctg
     7081 atcggtgtca ctctggttcc cctgctgtcc gggctgctgg gctgatcctg gcctttgtcc
     7141 ctgtccaaac ccttaactaa tcgcctccgc gtcctcccgg ccagaccggg agggcgcggg
     7201 aaaggaggtg ctacccgtga ttagtgaaat cattgaagaa tggataaaag gcatcctcat
     7261 ggacggtatc acgggcaacc tgtccgggct gttcgatacg gtaaatgcca aagtcggaga
     7321 aatcgccgcc gacgtgggac gaaccccgca ggcatggaac agcggtattt tcaatatgct
     7381 gcacagcctg tcggaaaccg tgatcgtccc catagccggg gccatactcg cccttgtcat
     7441 gtgctatgag cttatccaga tgataacgga aaagaacaat atgcacgact ttgacagctc
     7501 catgttcttc cgctggatat tcaaatccgc gtttgcaatc ctgatcgtaa cgaacacatg
     7561 gaacatcgtc atgggcgtat tcgacgccac ccaaagcgtc gtcaatcaaa gcgccggtgt
     7621 gattatcggg cagacatcca ttgattttaa caccctgata ccagaccttg aaagccaatt
     7681 ggaggctatg agcgtgggcg ctcttttggg gctgtggttc caaacccttg ttgtgggcct
     7741 caccatgaat atcctttcca tctgcatttt ccttgtgacc tatggccgca tggtggaaat
     7801 ctatgttgtc acgtcgctgg ggcctatccc ccttgccaca atgggcaacg cggaatggcg
     7861 cggcatgggc caagggtatc tgaaatccct gctggcgctg ggcttccaag cctttttaat
     7921 catggttgtg gtgggtatct acgcggtgct gatacagaat atcggttccg cttccgacat
     7981 atccggcgca atctggggct gtatgggcta tacggtgctg ctgtgcttct gcctgttcaa
     8041 gaccggcagt atatcaaagg ccgtattcgg ggcgcattga tcccaaaatc caagcataca
     8101 caggaggaac ttgtttattt caacgtacaa catcatttac gctgacccgc cgtggcggta
     8161 tgactgcaaa aacgtacagg gcgcagcgga aaatcactat tccaccatga gcattgacga
     8221 gctgtgcgcc ctgccggtgg aacggctggc gtccaaagac tgcctgctgt ttttgtgggc
     8281 cacattcccc atgctgccgg aagctctgcg gttaatcaaa gcgtggggct tttcattcaa
     8341 gaccgtggct tttgtatggc tgaaacagaa ccgtaaaagc cttacatggt tttacgggct
     8401 gggccgctgg acaaggggaa acgcggaaat ctgcctgctg gcaacacacg gcaagccgaa
     8461 gcgtcgttct gcctctgtcc atcagtttat tatcagcccg attgaacaac acagcaaaaa
     8521 gccggatgtg acccgtgaaa aaatcgtaga gctggcgggc gatttgcccc gcgtggagct
     8581 gtttgccaga cagaaaaccc ccggctggga cgtgtggggg aacgaggtgg aatgtgacct
     8641 tatcctgacg tgaaaggagg tgccaaatgg cttatgtaac cgtccccaag gacttaaccc
     8701 atgtgaaaag caaggttgtg tttgggctta ccaaacggca actgatctgt ttttccgggg
     8761 cgctggtgat cggcgtcccc ttatattttc tcacccgtga ctatcttacc aacagcgcgg
     8821 cggccctgct catggtattt gccatgctgc cgggcttttt gcttgccatg tacgagcgtc
     8881 acggccagcc ccttgaagtg gtgattatgc agatcatcca gtgctgcttc atccagccaa
     8941 aggagcgtcc ctatcagacc gacaacacct acaccgccct tgtgcggcaa taccagatgg
     9001 aacaggaggt aaaggccatt gtccaaaaag gaaatcccaa gagaaaccga aaaaacaaag
     9061 ctgacccgcg cccagcgaaa agaaattgac gcggtaatcc gtaaatataa gggcgacggc
     9121 aggccccaca cggcgcagca gagcatcccc tacgaggtca tgtacccgga cggcgtgtgc
     9181 cgggtatcgc ccggcgtctt ttccaagtgc attgagtttg cggacatcag ctatcagctt
     9241 gcccagccgg acacgcagac ggctatcttt gaaaaactgt gcgacctgta taactatgtg
     9301 gacgcctcca ttcatatcca gttttctttc ctcaatcgga aagttgaccc ggtgcagtac
     9361 gccaagagct ttgagatcgc gccgcagggg gacgacttcg acgacatccg ggccgagtac
     9421 accggcattc tgcaaaagca gcttgcaaac ggcaacaacg gcatggtaaa gaccaaatac
     9481 ctgacattca ccatcgaggc cgaaagcgtg aaagcggcgc gagccagatt gaagcgcatc
     9541 ggctttgacc tgttaggcta tttcaagagc atgggggccg tagcccatgt gatggacggc
     9601 tgggaacggc tgaacctgct gcatggcgtg taccacccgg acggcgagat attcaatttt
     9661 gactggaagt ggctggcccc gtccggcctc tccaccaagg attttattgc cccgtcctcc
     9721 ctgtgcttcg gcaatgcaaa gacattcggc atgggcggca aatacggcgc ggtaagtttc
     9781 ctgcaaatcc tgtccccgga gctgtcggac gatatgctgg ccgacttcct caatacggaa
     9841 agcggtgttt tagtcaatct ccatgtgcag gccattgaac agaccaaggc gatcaagacc
     9901 atcaagcgca aaattaccga ccttgacgct atgaagattg cggagcagaa aaaggccgtc
     9961 cgcagcggtt acgatatgga cattcttccc agcgaccttg ccacctacgg cgaggacgcc
    10021 aagaagctgc tgacaaagtt gcagacccgc aatgaacggc tgttccagtt gacgttcctt
    10081 gtcctcaacg tggccgacac caagcaaaag ctgaataacg acgtgttcca agccgcaggc
    10141 gtggcccaaa agcataactg tccccttgtc cggctggact accagcagga acagggcctt
    10201 gcaagcagcc tgccgctggg cgtcaatcag atcaagatac agcgcagcct tacgacttcc
    10261 agcgtggccg tgttcgtccc ctttgtgacg caggagcttt ttcagggcgg cgcggccatg
    10321 tattacggca tcaacgccaa gtcccgcaac atgattatgc tggacaggaa gcaggcccgt
    10381 tgccccaatg ccttgaagct cggcaccccc ggcagcggca aatccatgag ctgtaaatcc
    10441 gagatcgtca gtgtgttctt aacgacgcct gacgatattt ttatttcaga cccggaggcc
    10501 gaatattacc cccttgtcaa acggctccac ggacaggtaa tccggctgtc gcctaccagc
    10561 aaggactttg tgaacccgct ggacatcaac ctgaattatt cggaggacga taacccgctg
    10621 gccctgaaat ccgactttgt gctgtcgttc tgtgagcttg tcatgggcgg caagaacggg
    10681 ctggaagcca ttgaaaagac cgtgatagac cgggctgtgc gcgtgatcta ccggccctat
    10741 ctggcagacc cccgcccgga gaatatgccg atcctgtccg acctccacaa agcgttgtta
    10801 gatcagcatg tcccggaggc tgaccgggtg gcgcaggccc ttgacctgta tgtgtccggc
    10861 tccctgaacg tatttaacca ccgtaccaac gtggacatcg gcaaccggct tgtgtccttt
    10921 gacattaagg agctgggcaa gcagctcaaa aagctgggga tgctcattgt ccaagaccag
    10981 atatgggggc gcgtcacggc caaccgaagc caaggaaagg ccacatggta ttttgccgac
    11041 gagttccatt tgcttttgaa agaggaacag acggcggcct attctgcgga gatttggaag
    11101 cggtttagga aatggggcgg cattccaaca ggtgccaccc agaacgtcaa agacctgctt
    11161 tcctccccgg aaattgaaaa tatccttgaa aacagcgact tcattacgct gctcaatcag
    11221 gcgtccgggg atagaaaaat ccttgcggaa cgcttgaatt tgtccaccga acagcagaag
    11281 tacatcgaca attccgaacc cggcgagggc cttttgatct ttgaaaacgt ggtgctgccc
    11341 tttaccaacc ccatcccgca caacacccag ctttacaaga ttatgacgac ccgcttaaac
    11401 gaggtggcgg gagtatgacc cggcgacgcc acaaaggagg tgaaccacca tgaaagagct
    11461 gaaagccaag gcgaaagtga cgcaggccat gacccgcgac gggctgacgc tggaaaatca
    11521 ggccaccggc gaggtggaga acatttccag ccgggaggcc gaacaggatt tgtccgcgcc
    11581 ggggccgggc ggcaccgccg ataagctgct ggaacgggcc gacgccatcc atgacggcca
    11641 taaggccaag aaagccgcga agcaggccgg ggctgtggcc gccgaggggg acgctgccct
    11701 gcaccggcca tccgcccggc tccaactgtc ggaggaagaa cgggccgacc cggatttaca
    11761 gccctatatc cgaaaatcgg acaaacgggc cgataagctg gataaggccc gcgccgccat
    11821 ccccaagaag cgcgtcccgg tcaaggaaac cgtctttgac gaggcaagcg gcaaagcaaa
    11881 gaccgtcctg cactttgaca aacaggacaa ggggcctccc tccctgaaac ccaatccggc
    11941 aggccgtcct gtttccgagg tttttctatt cggccacggg aaaatccatg aagtcgagca
    12001 tgaaaacgtg ggtgtggagg gcggccataa gggggaggaa cttgccgagc ggcaggccgg
    12061 aaaagccatc cgcagcacca tccggcacca caggctgaaa ccctactggg acgcggaaaa
    12121 ggccgagcgc aggctattgt ctgccaacgc ggagtatttc taccagaaat ctttgcggga
    12181 taacccggag atcgcacagg cggccagcaa ccccatttcc cggttctggc agaaacagca
    12241 gatcaagcgc cagtacgcca aggccgcccg gacagcgggc cagaccgcag gcgcggcggc
    12301 acagggcgcg gccaagacag ccggggcctc cacgaaagcg gccaaggccg cagccaaaga
    12361 aagtgaacgg gcggcctcct ttgtggcccg ccactggaaa ggggcgctgg ccgtccttgt
    12421 gttggggctt ttgatgctgc ttatcatcgg cggcttacag tcctgcaccg ccatgttcgg
    12481 cagcgccgga agcggcatca cggcgtcctc ttacttttcc gaggatagcg atatgttggc
    12541 cgccgaggac gcctacgcgg agctggaagc cgagctgcaa gactatctgg acaactacga
    12601 ggccacccat gactatgacg aataccattt tgaccttgac gagatcagcc atgaccccta
    12661 tgtgctgatt tccattctgt cggcgctcca tgacgggata tttaccatcg gggaggtaca
    12721 gggtgatttg tccatgctct ttgacaagca atatatcctg acagaaaacg tcgtggtaga
    12781 aacccgttac cgcaccgaaa ccgatacatg gacggacgcg gagggcaaca cccatacaga
    12841 cacctaccaa gtgccgtatg actattacat ctgctacgtc acgctggaaa acttcgacct
    12901 gtcccatgtg ccagtctata tcatggacga ggaaacgcta agtttctatt ccctgtatat
    12961 gtccacgcta ggaaaccggc ctgatctgtt ttccggcagc cagtacccca acgcctccac
    13021 cctgacgccg ccgacctact acgacgtacc cccggaggct ttggaggatg ccacctttgc
    13081 cgccatgctc aaagaggcgg aaaaatatat cggttatccc tatgtgtggg gcggcagctc
    13141 tcccagcacc agctttgatt gcagcggctt catttcgtgg gtaatcaatc atagtggatg
    13201 gaacgtggga cggctggggg cgcagggcct atataacatc tgcacccctg tttccagttc
    13261 gcaggccaag cccggcgacc ttgtattttt caagggaacc tacgacaccc ccggcgtgtc
    13321 ccacgtcggc ctgtatgtgg gcaacaatgt gatgctgcat tgcgggaacc cgatttccta
    13381 cacgaacctt acttcaaact actggcaatc ccatttttac agttacgggc gtttacctta
    13441 acgccagaaa ggagctttca attatggcaa ccaaacttga ccgcatcgaa aaggacatcc
    13501 agaaaaccaa gtctaagatc gcggagtttc agaaacagct tcgggagctg gaaacgcaga
    13561 aaaccgaaca ggagaatttg cagatcatcc agcttgtgcg cggcatgaac atgaagccgg
    13621 aggaatttgc cgcgttctta cgcagcggcg caatgcaggc cgcccctgcc gcaacccctt
    13681 accatgaaca ggaggacaat gcccatgaag aataagacca gacggatttc ccgtattctc
    13741 cccgcacttc tggccgtgat gctctgcgtc acggcctttc ccgttaccgc ccttgcaggc
    13801 ggcaatgacc cggcaccggc cccgctgccg gaggctacaa ccgaacccgc tacgggcggt
    13861 ttggagcctg aaacagacga aaccggcacc gccacggacg ggacagaaac cgaggccgaa
    13921 aaagataccg gccctctgac cggcaaggac atcaccgacc tgttttccgc gctgttcggt
    13981 tccaaggtga gcattgccgc cacggacgac ggcatccaga tcaccaccgg caccgacaaa
    14041 aaagaacctg aacagaccgg caccgtcacc accaacggcg gcaggctgaa tgtccgcacc
    14101 ggcgcggggc ttgacaagac cgcgttcacc cagcttccca atgggacgac ggttgaggta
    14161 gtcggcacag acggcgactg gattaaaatt ctcttgccgg agcgcatcgg ctatgtccat
    14221 tccgactata tgaccgtcag cgaaaaggaa gtagcggcta ccggcgaggg cggtttttcc
    14281 ctctccattg acccggagga aattgcttct ctgctggaac tgttcaatgg cggcggggcc
    14341 agcgccgccc tgtccccgga cgggaacctg tcactgattg acgacatcgg cagcgccgcg
    14401 aaaaatggca agcagtttat caccgtggaa accaaaaacg gcaatgtgtt ttatctgatt
    14461 attgaccggg acgacgaggg aaaagaaact gtccatttct taaatcaggt ggatgaagcc
    14521 gatctgctga cgctgatgga ggacggggaa accgtcaccc ccgctgctgt ctgctcctgt
    14581 accacgaaat gcaaggccgg ggctgtgaac acgaattgcc cggtatgtaa aaccaacctg
    14641 accgagtgca gcggcccgga gccgcaggaa ccccagccgg aggaacccga agccccggag
    14701 gaagaaccca agggcggcgc gggcggcctg atcgtgttcc tgctggtggc gctggcgggc
    14761 ggcggtgcgg ccctttacta tttcaagttc aaaaagccca aggctgaaac caccggcagc
    14821 gacgatttgg gcgagtatga ttttggggag gacgaggatt tggaggacga ggaacccgaa
    14881 accgagtttg acccggaagc tgattttgtg agtgaacagg aggacgagga atgacatata
    14941 aacttgtaat cgcggaaaaa cccagcgtgg cccgcagcat tgcgggcgtg atcggggcca
    15001 cggagaaaca cgacggctat ttggagggga acggctatct ggtgagctgg tgcatcggac
    15061 atctggtgtc ctttgccgac gccggacgct atgacgagcg tttcaaaaaa tggcgctatg
    15121 aggatttgcc cattctcccg gaaacatggc agtacatcgt ccccgacgac aagaaacagc
    15181 agtttgacgt tctctgttcg ctgatgcagc gccccgacgt gaccggcctt gtgtgcgcca
    15241 ccgacgccgg gcgcgagggg gaattgattt tccgtttcgt ctaccagatg gcgggatgcc
    15301 aaaagccctt tgagcgcctt tggatttcct ctatggagga cagtgctatt aaggacggct
    15361 ttgcccacct gaaaccgggc ggcgactatg atccgctgta tcaatcggcc ctgtgccggg
    15421 ccaaggccga ctggctgatc ggcattaacg ccacccggct tttttccgtc ctgtaccaca
    15481 agaacctgac tgtgggccgc gtccagacgc cgaccttaaa aatgctggtg gacagggacg
    15541 ccaagattac cgatttcaag aaagagaaat atcacatcgt ccatatcacg gcgggcggcg
    15601 cggacgcggt aagcagccgc ttttccgacg ctgccgaggc aaacaccgtc aaggcggctt
    15661 gtgcaaaagc gcaggccgtt tgcgtttccg tgacgcggga gaaaaagacg gaacagccgc
    15721 ccaagctcta tgaccttacc accttgcagc gggaggctaa ccggcttttc ggctataccg
    15781 ccaagcagac ccttgactat gcccaggcac tgtatctaaa gaagctgctg acctatccga
    15841 gaacggatag tcaatatctt accgacgata tgctgcccgc cgtggaaagc cttgtgtccg
    15901 gcctgtggga gctggtgccg tttgccaagg gcctgaacct ttccccgcag tttgaccgca
    15961 tcctgaacag caaaaaggtg tccgaccacc acgccattat ccccaccgct gaatttgtga
    16021 agcagggctt tggcactctg gccgagagtg aaaaaaagct gctgtccctg atctgctgca
    16081 agctcttatg tgccgtggcc gggccgcata tctatgaggc cgtcaccgcc acatttacct
    16141 gtgccgggca ggagtttacc gccaagggca agaccgtcct ttcccccggc tggaaagaga
    16201 ttgaacgccg gttccgttct tccttaaaga ccgacgccga cgaggacgcc gaggcggtgc
    16261 gggagctgcc ggagctttgc gagggccaga cctttgcaga cgtggccgcc tctgtcaccg
    16321 agcatttcac cacgccgccc aagccctaca ccgaagatac gcttttgtcc gctatggaac
    16381 gggccggggc cgaggatatg ccggaggatg ccgagcgcaa gggattaggc acaccggcca
    16441 cccgcgccgc cattctggaa aagctggtgc agatgggctt tgtccagaga aaaggcaagc
    16501 agctcattcc caccaaggac ggcatcaatc tggctgtggt gctgccggag gcgcttactt
    16561 ctccccagct tactgccgaa tgggaaaacc ggctgaccga gatcgccaag gggcaggccg
    16621 atcctgacga atttatggac ggcatcgaga cgcaggcgcg ggagctggtg cagacctatt
    16681 cctgtatcag tgaggaaaag cagaaattgt ttcaggccga gcgtgttcct atcgggacgt
    16741 gtccccgctg tggtgaggct gtctatgagg gcaagaaaaa ctactactgc ggaaaccggg
    16801 cctgccagtt cgtcatgtgg aagaatgacc gattttttga ggaacgcaag aaagcgttca
    16861 ccccgaagat cgccgctgcg ctgctcaaaa ccggcaaagt caaggtaaag ggcctctatt
    16921 ccgtaaagac cggcaaaacc tacgacggga cggtgctttt ggctgatacc gggggcaagt
    16981 acgtcaacta ccgcattgaa cacaggagct aaaggacagt aacacagcaa gcatagggag
    17041 tgtggctaca ctcccggtaa aatcccccgg ccgcgtccct gccgggccgg ggatttttgc
    17101 ttgttgggga agtccccaac cccctatttt ttgaaacaga acagagaaag gagtgaccgc
    17161 gtgaacgaag tattttctat ccaagtccat aaccggcagt ttttggaaca gggcaaacaa
    17221 ggcgtctggc tggatttgcc caccacaacc gaaacgctgc aagccgccct gcgggatgtt
    17281 ggcattactg ccgacaatcc gcaggatttt tttatcaacg ggtattcctg cccggaggac
    17341 agacaccttg ccattcccta tgacatggtg ctggccgccg acgtggacga gctgaatttc
    17401 cttgccgccc ggctgggcca gcttgacgcc gccgagatcg cagagctgaa cgccgccctg
    17461 caaaatcccc gtggcggctt tgagaatatc ggccagatca tcgactacac cgaaaatgtg
    17521 gattactatg tgcatcttcc cgacgttagg ggggatggac agttgggcga ctactacctg
    17581 aaccgttccg gcatggtgga tatgccggag gaatggaaag ccgggattga tacggcgcag
    17641 tttggacgcc acattgccca gcaggaacaa ggcgttttta cccagtacgg ctatctggtg
    17701 agaagcggcg acgaatggca gcgcgtccat gagggccagc cggtgccgga ggaataccgg
    17761 gtgatgcact acccccagcc ggaagctgac cgggaggaac cgcaggccca gcagccggag
    17821 gcatccgaaa gccccgacaa agcgccgccc ccggttatcc cgatcatgct ggacggcatg
    17881 aacagcaaag aacgcatgaa agtgattact gaccgtctgg aaaccggcgt acaggagctt
    17941 tttgaaagta gccgctatat ggcttactta tccgctatgt ccaagatgca cgattacagt
    18001 ctgaacaata cgctgctgat cgccatgcag ggcggttcga ttgtggcggg ctataacagg
    18061 tggcaggatg aatttgaccg ccatgtgaag cggggcgaga aaggcattaa gatactggcc
    18121 ccggccccgt tcaaggttaa gcagcagatg gagaaaaccg acccggccac ccagcagccc
    18181 gtgatcggcg cggacggcaa gcccgtgacc gaagaacagg aagtgacggt tccggccttt
    18241 aaggtggtga cagtctttga catcagccag accgagggca aggattttcc catgccctat
    18301 atcagtgaac ttaccggcga cgtgcagcag tatcaggatt tctttgccgc gctggaacgg
    18361 acatcaccct atcccataag cattgagccg atttcagacg gggccaaggg ccgctgcttc
    18421 tacgcaaaac agcgcattgc catcaacgag ggcatgagcg aattgcagac cctaaagacc
    18481 gccatccatg agattgccca tgccaagctc cacgccattg accgggaggc ccccgccgag
    18541 gaacaggcgg cccggccaga ccgcagcacc cgcgaggtac aggccgaaag cgtggcctat
    18601 accgtctgcc agtattacgg gctggacacg tcggactatt cttttggcta tgtggccgat
    18661 tggagcagcg gcagggagtt atccgagctg aaagcctccc ttgaaaccat ccgggccacg
    18721 gccagcgaat tgataaccga gatcgacggc cattttgccc agcttcagca ggagcgcgag
    18781 gcccggcagg aacaggaggc cgtcacggtt gacgttaccc cggaggcagc ggccccggcg
    18841 caggccgagg aagaacaggc cccggccctt gatccggcag ccgagccgct tgtgactgtg
    18901 ctatggagcg aaagccccca gctaaaggac ggccagcaaa tgccgttgca tgaggccgac
    18961 gcgctgtttg cccgtctgga tgcagcatat ccggcagagg caggctatga caaaaccgcg
    19021 ttccgcattg actgtatctt tcagggcgag ccggacagct acgaggggcg gcaggatttt
    19081 ggagataggg acggttccct gatcgaacat atccagagct accaccaatt ttacgcgcag
    19141 gacgaaaact ggaaaaagca tgtaatcgct acaaccggca tagagggctg ggagcaggac
    19201 aaggcccatc gggatatgct gttaggcgag tttgtcccct atatgaagct gcattgcagc
    19261 cttgcccaaa tggaacagac ggcgcagggc cgcttacagt ccggggaaac cctgacgccg
    19321 gaggaaacgg cctatttggg ggcgcttctc tcttatgtgc aggagtgccg ccccatgctc
    19381 aatcagggcc agtatcagtt gccggagccg ccgaaactgg ctgacttcga ccagagcttg
    19441 caggactaca aagcacaggt acaggccgag atcgcgcagg aggccgccgc agccggtatg
    19501 accgtggagg aatacgcggc ggctggctat gaagcacccc aacaggacac cttttccatc
    19561 taccagcttc gaggcgggga ggaaacccgc gactatcggt ttgagccata cgagcgttta
    19621 caggccgcag ggctggccgt ggacagtgcc aactatgacc ttgtttatac ggcaccgctg
    19681 gataaaagca cgtctttgga ggatatttac cgcacattca acatcgacca cccggcagat
    19741 tttaggggac acagcctttc cgtttccgac gtggtggtgc tgcaccagaa tggacaggac
    19801 accgcccact atgtagacag cttcggctat atggaagtgc cggagttttt gcgggaaaat
    19861 cccctgcgta ccgccgaact gtctacggag cagaacgaaa acatgattga cggcattatc
    19921 aacaatacgc cgcccgaacc gcagccggag gaacccgaac agctatcttt tgaggatgtg
    19981 gcagttgctt ccggccagac acaggaggaa cccccggagg ctgcgccccc gcaaacggct
    20041 gtccgctatt atcccatcaa tgagggagcc gcccgccgcg ccaaggaagc catgagcttt
    20101 tctgactacc ggcccggcag cgccacggcg gaataccggc actatgtcga tcaggccgtg
    20161 gaaattgcgg agcgccagaa aaagcgtgtg gagccagagt atcacgaaaa gatagacagc
    20221 ctccttgaca cctacgcccg caagctggcc gagaacatga ataagggcta tgagattacc
    20281 gcccgcgtcc cctctgtcct gattgccggg ccgtccaatt tcccggtacg ggcgaaagaa
    20341 aagcagaacg ccgccagtga ccgcaacatg gaggaatttc agtacattca gggcttgctt
    20401 gacaaaatcc gcagcaccgg catgggtggt atcagcgccg acgatccgca ggccgtttcc
    20461 aagctggaaa agaaactgga aaagctggaa gcgtcgcagg agttgatgaa ggccgtcaat
    20521 gcctactacc gcaagcatgg gacgctggac ggatgccccc atctgatcga aagaggcatt
    20581 gaaaacctga aagcggatat ggcgtcgggc tggcactatg agaaaaaacc gttccagtcc
    20641 tggcagctct ccaacaataa cgccgagata cgccggttaa agggccgcat tgaggaattg
    20701 acccgccaaa aggaggcggc ctatgtggga tgggagtttg acggcggcac tgtggagatc
    20761 aaccgggtgg ccaaccgcct gcaaatcttc tttgagggga agccggatgc tgctgtacgc
    20821 gacgagctga aaagcaacgg tttccgctgg tcgcccaagg ccggggcatg gcagcggcag
    20881 ttaaacgaca ccacaatccg cgtcgctgac cgtatcaagt gcatccagcc cctttccggc
    20941 gaaaagccca gcgccttaca gcttgcggcc cgcagggaga aagaaaagcc ctctatccgg
    21001 gcgcagctca acgcggccaa gaccgaacag gccaagcagc caccggcgcg ggaaaaatca
    21061 aagggattgg aggtagagta aatgcagaag tttgaaaatg tggacgttct ggccgcgctg
    21121 gaacagatca tgcggcagaa caccgcgttt tatcaaagtg actttggcat tgacaaagac
    21181 attatcaggc gggatgccgc cagcgatcag gccgcagaca agaccctgct ctggatgtcc
    21241 cggccctccg ggacgtactg tttccgggaa cgtgatgttt tcctgaaaga cacccgacag
    21301 tacaacacat ggaaatttta cggggagcag acccgtgaca agatactggc ctacgcggtg
    21361 gagctgaccg gcacacaggg cggcactatc cggggcaatc tctatgagct ggactatccg
    21421 cagcatttcc ggcacgtcat tgaaaccgcc cagccggtga gtgtgaaccg gctcttttat
    21481 gagcatggca cccgtgacat cccggagagc cagcgttttg acggaagccc tgaccgggta
    21541 ttgggaaact ttttgtacta tgaggcccag ccccatgacc cggctgtgct gcaggaggcg
    21601 ctacggacgg agcagcacag ccgggggcgg gccgtccccg gcgatttcaa agtccacgtc
    21661 accgctttgc atgacagccg gattgaaacc gaggcccggc gtattgtgga cagcctgaaa
    21721 gatctgtccg cgcctaacag ccccaataaa acgcatttca tggtggagat ctccccgcac
    21781 tttgcggcga ttgcttccac caaggataat gaccggctct ttgccatgct gccctataaa
    21841 tccctgtgtt ttaccgggat gaaaggcagg catggcgtgt atgccgtgat taaccgggac
    21901 gagcgccggg atgtgagtat tcgcaggccc cggccctcta tccgcaagca gctatccgac
    21961 agcaaacagg ccgccagccc aaaaaaggcg gcggccagaa ccaaaaagaa tgaattggag
    22021 gtataaggaa tgatgcactt taccgtggag gaagaaaacc tgatctgtat gtaccacaat
    22081 gcagaccggc gcagaacgat aaacaggctc accgccgtca tgccggttat ggatggggat
    22141 atgcgggcgc aggcccagca gaccctttcc aagctggaac gcatgacgga tgccgactat
    22201 gacggccaga agttccactt cacagacgaa taacgaacag cagacgccgg gcgcggcggc
    22261 catgtggtcg cttgcgtccg gcgtcttttc tttttgccta ttcctacgca tttagaaccc
    22321 ggtattgtcg gggataggat gaagtattcg cttgcccctg caaacatgcg ggagaagcct
    22381 tgcagcgggc ggttttcaat cccttaaatg cgtaggttgc agctataatt agacccctga
    22441 aacagatata gccgcgcagc gggtgtatct gtttcagggg tgtgcaggtg atagccgcaa
    22501 agcggcgtaa gagggtgcag ccctcttgcc gtctatctct gttccagcgg gaagctcggc
    22561 aatgcttcca gcagtttcag ggaaatgcgc tgccgtatat cttcgtcaat gactttcttc
    22621 tttttgccgt ttgcctgtct gatctccacc gtggaaagct cgttgatgta gtcagcataa
    22681 tgggccaaca ctttttccgt ggcccacctc tcaccggcaa cggcggcttt gatcgtttca
    22741 tagtccagca gcttttcttg tccgtccatc ctgatacccc cgtcagactg attttttcta
    22801 tgataccata ttcggaagca tggaaccaga gaaaacggcg cttttctgct ggtaaattct
    22861 acgcatttag aacagcactt cggcggggat aggataaaac acccacatac cccgctgacc
    22921 atgccggaaa agccttgaaa tataagggct tttacagctc taagtgcgta ggtttccagt
    22981 atcttttccg ctggcgctcg gcctctttgc ggcggcgcat ccgtttggag caggccgcgc
    23041 agtatttggc ccggttagac ttcggcgtga acgtggcccc gcactcacag cagcgtttca
    23101 cttcgtcccg gtctttggtg atctcggcat acagggccgc atccagcggg aggacagcgg
    23161 ccttaaacca gcggcacaga agcgaatagg aaatgctctg tacgcaaata cattcttcgc
    23221 cgtcgtccag caacaggcag ttgccgtcac aatagttgca gcactcatgc gtcagcttcc
    23281 gggccttgcg atattgaggg taattcattc gtggaatact catagctctt gcgtccattc
    23341 ctttcccggc gtcatacgca aaatgccgtc cacattctgc ttgatgatgc cgtattcctg
    23401 catctgcttt ttcgcttcgc cgtacttctc atacagccgt tccttttcct cggccaatcg
    23461 cttgtactcg gctttcaggg cgtacagatc ggggagcttt ttcacaccct gtccctttag
    23521 ggcctttgcc gcagcttcat acaaaatcaa cgtgctttca tgctcacgcc ggaattttgc
    23581 cttatccgct gcattgcggt attccatagc cacgggccag agctgcttgt aggtagaaat
    23641 attctttatc agcaccgcca tatccgcaag ccggtgttcc atagctttga gggtagcggc
    23701 ggcctcgtcg ctggcggcgg tgatctcggc cactttgctt tccaaatccg ggtaggtgtc
    23761 tatcccatgt tcagtcagga aattcaaagt ctttgcggcc tgcttcaaat tgtggagctt
    23821 cgcccacctc tcatagcctg ccgactgctg ggccttaatg ttgttttcca gatcaatccg
    23881 caaagaaatc cccttgcgct ctttgggcgc tctggtgata acgcggcctt tgatacgctc
    23941 tttgatggct tcctcggtgt aggcttcccc caatgtcttg cagcgggtaa agcgttcctg
    24001 tccgggagcg cggaaagaaa taaactttcc cggcttgatc tcatagccct gcgcctgcat
    24061 gagccgcagg aaatcatcaa aatcttttgc ctgcgggata gcggcgtcaa tggctgcttt
    24121 gagcttcgct ttccagctcg tccctttccg ctgggcgtcc cactcggcat agctcttgcc
    24181 cttactctgg ccgggcttca ccacggacaa tccgttttcc ttgcacagcc tgtcgctggt
    24241 gcgccggata taggcatagc tctgtttgtt ggaaatgtac ttgcgctgat ttaccatatc
    24301 cacggcacag aaaataatgt gattgtggac gtggccctta tcaatatggg tagtcagcac
    24361 atagggatat ttcccttgca gcacttcctc ggcaagctgc ttcccgatct catgggcctg
    24421 ttccggcgtg gcttcgccgg gggcaaagga ttgtatcagg tggtggccca gattattgcc
    24481 cttttggtat gcctgcgcca gcagcatttc aaattcaatg tccgctgttt cataagagca
    24541 ggcaaaggag gccaccagca gcttgccgtc agttttgtcc gggttctcga tgtagtcaag
    24601 ggcttttttc agcgttgact tgataggatg tatctttgta actgccatat ctgcgccagc
    24661 gcccccttta tatcctcgat gtcctgtgcg tacaccggcc ccataccgtt cagcctcttt
    24721 gcaatctgat tgacgttgac gccgatcttc tgtatctcgg cggtctgcgc ccggatgtcg
    24781 ctggtgtcca tgttgacgat atagccgtca atgagcatct tccgggcgta ggctgaaaag
    24841 ttcttcgtat gcagcagcgc cattttctgt tcaatcatag cccgttcctc cggcgtcaca
    24901 gggacgcgca gcacgatttt tcgctttctg tttgccatgc ttcaccgtcc tttccattca
    24961 ggggtttggg gatttcccca acaagcaaaa cggacgcggc ccggcaggga cggggcaggc
    25021 cgtttttccg ggagtggcta cactccctat gcttgctacg ttactgtttc tccactaaca
    25081 agacgtttcc aaagccccaa actacccgca aaatagccct ctggaaagaa aaaagtgcaa
    25141 aaaaatagaa gctacgctgt gcagctcccg ctaaaagtaa aacagaggat gccagttaca
    25201 catcctccgt ttcattcttc ttcggctttt caattagtat ggcgctgtca atcgcgccct
    25261 cgatgatcgg caggtattct tcggggcaga gtttcagctt gtggctgacc ctttgccgca
    25321 gttcgctttc ctcccgcatg atctccgggt taaagtagcg ttccacagga agcccgcagg
    25381 ctttaataag ctgtatgaca accggcagac tgggaatggc ccgtcgtttt cgatgttggc
    25441 aagatagcgc cagtcaatat ttattttctc tgccattgct ttgcgcgaaa cgcgctttgc
    25501 ttgccgggcc gctttcacat ccgcgccgaa agtttcaaag ccgggacaat cttcaatatt
    25561 cgccatatcg cacctcccac ttacattgta tatttcatac tctgcccgtg gaatgttggt
    25621 atatgcacct ttcttaaagt ttatatttca cattttgttt cttttattct tcgagcaaaa
    25681 gaattataat gtaccctgtg gaggtgtact atggactaca tgacgttgaa agaggcaggc
    25741 gagaaatggg gtgtgccccc acgatggata aactactatt gtgctgcggg acgcatcccc
    25801 ggcgctgtaa agatggcgac gatctggctc attcccaaaa ctgcggaaaa gccgattgac
    25861 aggcgtacaa agaaaggaaa ggcggttact gaatagtata atgaaaccgg cgcattttgg
    25921 atatggtttt gaagagagag aaagcatggt attttgtcac ggaaaaacta taagatgata
    25981 gatggcaggc tgctccagac cgataaaaag tattctcatc tgaaacagaa agaaaagatt
    26041 tacacatgga tgtatgaaga agcgaaactt tatcatgaca gaaatggaaa atgcccgaaa
    26101 caaagacgag gaagctgctg ctgtatcagc cgtatatggt agaattgaaa aggccggcat
    26161 ctggattctt tatggtgaag tctccagaca ttggataact gctggtctaa tgcgcctttg
    26221 agtatggggt gattgatttt aataccgggg aggtacggct ttttgaggaa aatattctgg
    26281 gatttacatt tggcgattat tacatttcct gtaactgcaa ggacacggat gatgaagata
    26341 tgaggaatat agacttgctg attttctact gtcttgaacg gtattaggac tgaatctgct
    26401 gcgcggagtg cggcaagagc gaccggaggg agtacacaca ggccctgcct gtgtgaaaag
    26461 ccataccgca aggtgcattc gcaaaaaaga ggccgcgcat tttgctgagg cttggggtgt
    26521 ccccaacaag caaaacgaga acgccgcagg cgggcaagtg gtggatcaga atatccgtaa
    26581 aggatattct gatccattgc ttgccagtat tatcgggatg ttttgacaac cgctgtaatc
    26641 gaaacatccc aaacattaag aaggtgttaa ttattattat cttgatttaa ttcgtttaaa
    26701 ccaataatat agatacatac accatgaaaa gaagcgagta tcccaatgac ggatgattaa
    26761 aatccgaaaa gaaatcgctt tgaaactaca gggaaactac agactgttat gttatcttct
    26821 taaaatggag ggatttttat gtcgatacga attctacttg tcgaggatga tgatcatatc
    26881 tgtaatacag taagggcgtt tctggctgag gcaggatatc aggtggatgc ctgcacagat
    26941 ggaaatgagg catacaccaa gttttacgaa aacacttatc aactggttat tcttgatatt
    27001 atgctgcccg gtatgaacgg gcatgaactt ttgcgtgaat ttcgtgcgaa aaatgatact
    27061 cccattctga tgatgacagc cctgtcggat gacgaaaacc aaatccgggc gtttgatgca
    27121 gaggcagacg actatgtaac aaagccattc aagatgcaga ttttactaaa gcgggtggaa
    27181 gccctgctgc ggcgcagcgg tgcgctggca aaggaaatcc gtgtcggcag gctgacactt
    27241 ctgccggagg atttcacggt actttgtgac ggtacagagc tgcccctgac acgaaaagaa
    27301 tttgaaatcc ttttgctgct ggtgcagaac aaaggcagaa ccttaacgca tgaaatcatt
    27361 ttgtcacgta tatggggata tgactttgag ggtgatggca gcacagtcca cactcatatc
    27421 aaaaatttgc gggccaagct gccggaaaat atcatcaaaa ccattcgcgg tgtaggatac
    27481 cgattggagg aatcataata atggaaagaa aagggatttt cattaaggtt ttttcctata
    27541 cgatcattgt cctgctactg cttgtcggtg taacagcaac actgtttgca cagcaatttg
    27601 tgtcttattt cagagtgatg gaattacagc aaacagtaaa atcctatcag ccattggtgg
    27661 aactgattca gaatagcgat aggcttgata ttcaagaggt ggcagggctg tttcactaca
    27721 ataaccaatc ctttgagttt tatattgaag ataaagaggg aagcgtactc tatgccacac
    27781 caaatgccaa tacatcaaat agttttagac ccaactttct ttatgtggta catagagatg
    27841 ataatatttc gattgttgct caaagcaagg caggtgtggg attgctttat caagggctga
    27901 caattcgggg aattgttatg attgcgataa tggttgtatt cagcctttta tgcgcgtata
    27961 tctttgcgcg gcaaatgaca acgccgatca aagccttagc ggacagtgcg aataaaatgg
    28021 caaacctgaa agatgtaccg ccgccgctgg agcgaaagga tgagcttggc gcactggctc
    28081 atgatatgca ttccatgtat gtcaggctga aagaaaccat cgcaaggctg gaggatgaaa
    28141 tcgcaaggga acatgagttg gaggaaacac agcgatattt ctttgcggca gcttctcatg
    28201 agctaaaaac gcccatcgcg gctacaagcg ttctgttgga gggaatgctt gaaaatatcg
    28261 gtgactacaa agatcattct aagtatctgc gcgaatgcat caaaatgatg gataggcagg
    28321 gcaaaatcat ttccgaaata ctggagcttg tcagcctgaa tgatgggaga atcgtaccca
    28381 tagctgaacc gttggacata gggcgcacgg ttgccgagtt gctgcccgat tttcaaacct
    28441 tggcagaggc aaacaaccag cggttcgtca cagatattcc agccgggcaa attgtcctgt
    28501 ccgatccgag gctgctccaa aaggcactat ccaatgtcat attgaatgca gttcagaaca
    28561 cgccgcaggg aggcgaggta cggatatgga gtgagcctgg tgctgaaaaa tgccgccttt
    28621 ttgttttgaa catgggcgtt cacattgatg atactgcgct tccaaggctg ttcaccccat
    28681 tctatcgcat tgatcaggcg cgaagcagaa aaagtgggcg aagcagaaaa agtgggcgaa
    28741 gcggtttagg acttgccatc gtacaaaaaa cgctggatgc catgagcctc cagtatgcgc
    28801 tggaaaacac ctcggatggc gttttgttct ggctggattt accgctcaca tcaacattgt
    28861 aaatatttaa aacttaaatg attttgggct ctttgtcaaa taggactgat agagtgagat
    28921 aagaacatct aaggtagaat tccttttcta ctttagatgt tttttgattc ataggtaatc
    28981 agattattta gtaagaaaat tctggtcttc attttccgaa agcttcgaaa gccataagcg
    29041 actcgtttca atgctttgat gtgggtatgc agattttcta ttttggcatt tgaataaggc
    29101 aattcgaggg ctaaacgaat gccggattca tagtttataa aagctttttt tatagaatgc
    29161 agttcttttg gaacggaatt gggcatcgtt cttacaagtt taaagaaagt gtccgcctct
    29221 ttgtcgtgaa acgcttgaag aatttcttga tagtaaaaat aggcgattcg taattcgtca
    29281 gagatcgaaa gcagacgatc caccacttca ctttcggtga gatacgccca accaaaattg
    29341 cgccatttgg tccatttgtc ataaacaagc ttgtcctgtc gtttgatcag aagccgagac
    29401 aacgccttta attgtcgata atatttggct tgtgttggat cgtttcgatc aaagcttttc
    29461 atgattcgta cgcgaatatc ctcaaattta cgaatgagat gtttgacaat gtgaaaacga
    29521 tccacaacga gttttgcctt tggaaaacat tcctttatca gagaggcata agaaacattc
    29581 atgtccgtca cgatgacctt taccttcaat cgtgcgcgtc gttcaaattg cataaaataa
    29641 gaaacgagag cctttttctt ccgtgaagga agaatatcca gtaattttcc agtatcgcca
    29701 tccatacaag aaaaggagta gggaccgact tgatttttca gtgaacgaaa ttcatccatt
    29761 aataaacaac gcggtaacca atttcttttt acttttgtca tggggatcag agaagcgagt
    29821 gtccgagaaa cggtcgacca agaaacgccg caatgcttag caattgtcgt cattgcttgt
    29881 ttttcagtta gctcttctaa aattttatag cgcacttgtt tagcgataaa gcagtttcta
    29941 tcgacaatgt aggtattcgc cgtaaaatag gtgtggcagc cacgacagtg gtagcgttgt
    30001 ttcgctaaag caagataagt cagttggttc gcacatttat taagttgaat gagcgatttt
    30061 ttctttccgt tcttaacgat catgccttca tgattcatct gtttacacaa aggacagcga
    30121 cgaatttctt tacttaacgt cgcagaaaca acaagactct tgatattctt aatgctcttc
    30181 gattccacat ttgtgatgaa aatgttttta tctttgattc caagtagtag tttgatatga
    30241 tgaagatcag gcatacagaa cgctcctttg attgtgttgt ggtgattcaa ttctaacgga
    30301 gcttctgtat gttttgtttt ttcatgctca aaagggaccg atagaatgat tttctatcag
    30361 tcctagaaat tatagagccg attttgaccg acaggtataa ccctgccggt ctttttttgc
    30421 tgctacagga aaactacaga ctggctacag ggaaagtaca gatgcgcttg ccataataac
    30481 aatcgtacca gccacaaatc gtagttttat tgcaaaggag gcattcgatc aaatggaaaa
    30541 aagcaactat cattccaatg cggatcatca caaacggcat atgaaacaat ccgtggaaaa
    30601 gcgggctttt ctatgtgcgt tcattatttc gttcacagtc tgcacgctgt ttttggggtg
    30661 gagactggct tccgtattgg aggcaacaca gataccgccc atccctgcaa ctcatacagg
    30721 cagcagcact gacgtagtgg agaatttgga ggaaaacgct cttgccaccg ccaaagaaca
    30781 gggagatgaa caggaatgga gcctgatttt agtgaacagg cagaacccca tccccgcaca
    30841 gtacgatgtg gagcttgagc aactatcaaa tggtgagcgg atagatattc ggatttctcc
    30901 ctatcttcaa gatttgtttg atgccgcaag aactgatgga gtttacccga ttgtcgcatc
    30961 cggataccga acaacagaaa aacagcaaga aattatggat gaaaaaattg ccgaatataa
    31021 ggcgaaaggc tacacctctg cacaggctaa agcggaagca gaaacttggg tggccgtgcc
    31081 gggaacgagc gagcatcagc ttggtcttgc tgtggatatc aatgcggacg gaattcattc
    31141 aacaggcaac gaggtttata gatggctgga tgaaaacagc tatcgttttg gttttattcg
    31201 tcgctaccca ccagacaaga cagagataac cggtgtgagc aacgagccgt ggcattaccg
    31261 atatgttggc atcgaagctg ccacagagat gtacaaccaa ggggtttgcc ttgaggaata
    31321 tttaaaacca gaaaaatgag aaaaggatgt tatgttatgg acagaaaaag attgacacag
    31381 cgtttcccgt tcctgcttcc aatgagacgg gcgcagagaa aaatgtgctt ttatgcggga
    31441 atgagatttg acggctgtcg ctatgcgcag acgatagggg aaaaatcgct ttcccatttg
    31501 ctctttgaaa cggattgcgc attatacaac cacaataccg gatttgacat gatataccag
    31561 gaaaacaagg tgttcaactt aaagctggca gcaaagacct taaacggctt attgataaga
    31621 ccgggggaaa ccttttcttt ctggttgttc ctgcatacgc cattgacaat tatccagcgc
    31681 agaggtcacg aagtaaagga gtttccagag ccaaacagtg acgagattaa aggggtggat
    31741 gcaaccattt cagagggctg gattgattta aaagtgcgaa acgataccga ctgcacctac
    31801 caaatatggg tgaccttaga tgatgagaaa atcatcggtc aggtgttcgc cgacaaagag
    31861 ccccaagcat tatacaaaat tacaaatggc agtatccagt atgtccgtga aagtggcggg
    31921 atttatgaat atgcccaggt taaacggatg caagttgcct taggtaccgg ggaaataata
    31981 gattgcaagc tgctttatac aaacaaatgc aaaatctgtt atcccctccc ggaaagtgtg
    32041 gatattcagg aggagaacca atgagaaaaa gtgtgggcat tactgttttt ggatgcgaac
    32101 aggatgaggc aaatgctttc cgcgctttat cgccggattt tcatattatc cctacgctga
    32161 ttagcgatgc gatatcggca gacaacgcaa aattggccgc tggcaatcaa tgcgttagcg
    32221 taagccataa gtccgaggtt tccgaggcga caattcttgc gctgagaaag gtcggggtaa
    32281 aatacatttc tacccgcagc atcggctgcg atcacattga tacgactgcc gccgagagaa
    32341 tgggaatctc ggttggcacg gttgcgtatt cgccggacag cgttgcggat tatgctttga
    32401 tgctgatgct gatggccata cggggtgcaa aacccaccat gcacgccgtg gcgcaacaag
    32461 atttcagatt ggatcgtatc cgggggaaag aactggggga tatgactgtg ggagttattg
    32521 gaaccggcca tatcgggcaa gcggtcgtca aaaggctgcg gggatttgga tgccatgtgc
    32581 tggcctatga taacagccga aaaatggatg cagattatgt ccagcttgat gagcttctaa
    32641 aaaacagcga tattgttacg ctccatgtgc cgctttgtgc ggatacccgc catctgatcg
    32701 gtcagaagca aattggagag atgaagcaag gcgcattttt aatcaacacc gggcgcggag
    32761 cgcttgtcga taccggggcg ctggtggagg cgttggaaag cggaaagctg ggtggtgcgg
    32821 cactggatgt gttggagggc gaggatcagt ttgtttatac cgattgctcg cagaaagtgc
    32881 ttgaccatcc ctttttgtcg cagcttttaa ggatgccgaa tgtgatcatc acaccccata
    32941 cggcgtacta cactgagcgt gtgctgcagg atactacaga aaaaacaatc aggaattgtc
    33001 tcaattttga aaggagttta cagcatgaat agaataaaag tcgcaatcat cttcggcggt
    33061 tgctcggagg aacatgatgt gtcggtaaaa gccgcaatag aaattgctgc gaacattgat
    33121 acggaaaaat tcgatccgca ctacatcgga attacaaaaa acggtgtatg gaagctatgc
    33181 aagaagccat gtacggaatg ggaagccgac agtctccccg ccatactctc cccggatagg
    33241 aaaacgcatg ggctgcttgt catgaaagaa agcgaatacg aaacacggcg tattgatgtg
    33301 gctttcccgg ttttgcatgg caaatgcggg gaggatggtg cgatacaggg gctgtttgta
    33361 ttgtctggta tcccctatgt gggctgtgat attcaaagct ccgcagcttg catggacaaa
    33421 tcactggcct acattcttac aaaaaatgcg ggcatcgccg ttcccgaatt tcaaatgatt
    33481 gataaaggtg acaagccgga ggcgggtgcg cttacctacc ctgtctttgt gaagccggca
    33541 cggtcaggtt cgtcctttgg cgtaaccaaa gtaaacggta cggaagaact taacgctgcg
    33601 atagaagcgg caggacaata tgatggaaaa atcttaattg agcaagcgat ttcgggctgt
    33661 gaggtcgggt gtgcggtcat ggggaacgag gatgatttga ttgtcggcga agtggatcaa
    33721 atccggctga gccacggtat cttccgcatc catcaggaaa acgagccgga aaaaggctca
    33781 gaaaatgcga tgattacagt tcccgcagac attccggtcg aggaacgaaa tcgggtgcag
    33841 gaaacggcaa agaaagtata tcgggtgctt ggatgcagag ggcttgcccg tgttgatctt
    33901 tttttgcagg aggatggcgg catcgttcta aatgaggtca ataccctgcc cggttttaca
    33961 tcgtacagcc gctacccacg tatgatggcc gccgcaggaa tcacgcttcc tgcactgatt
    34021 gacagcctga ttacattggc gttaaagagg tgataagtat ggaaaatggt tttttgtttt
    34081 tagatgaaat gttgcatggc gtccgttggg atgccaagta cgccacatgg gataacttca
    34141 caggaaaacc ggtggatggg tatgaggtga atcgcatcat cggcacaaag gccgtggcgt
    34201 ttgctctgcg cgaggcacaa atccatgcgg ctgcgcttgg ctatggcttg cttttatggg
    34261 atggatatcg gccaagaact gcggtggact gcttcctgcg ttgggcagcg caaccggagg
    34321 acaatctcac aaaagaaaaa ttttacccca atatagagcg agccgagttg attacaaagg
    34381 gttatgtggc ctcacaatcc agccatagcc gtggaagcgc aattgatctt acgctctacc
    34441 acctggatac aggggaactt gtttcaatgg gaagtaactt cgattttatg gacgaacggt
    34501 cgcatcatac agcaaaaggg ataggggatg cagaggcaca aaatcgaaga tgcttgcgta
    34561 aaatcatgga aagcagcgga tttcagtctt atcgctttga atggtggcac tataagttga
    34621 ttgatgagcc ataccccgat acctatttta attttgccgt ttcataatga aagtattggt
    34681 tttgtatcag attttggatt tgataaaatc tatgctggtc atggacaatc ttttactgga
    34741 aagtggttaa agcatctttt gtaatacatt gcaaattata gtattcaatc tgcccagcgg
    34801 cagaaaagaa aaaaaccgct gttgggcaga cctttcccac gcccattttg tattttgctg
    34861 gcggcggttt tgagaaatca aggccgccgt tcttttctgg cagcaaaagg tgtggcggcc
    34921 cataggagga tgccgctatg ctgttttgct attgccatgc tctaatccac ttccaccact
    34981 caaaaaaagc cttgcccctc
//
LOCUS       DAYYME010000013        34795 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000013 DAYYME010000000
VERSION     DAYYME010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.013"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACHBFW_000526"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACHBFW_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFM9316867.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACHBFW_000527"
     CDS             complement(2020..2922)
                     /locus_tag="ACHBFW_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9316868.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACHBFW_000528"
     CDS             complement(2919..3611)
                     /locus_tag="ACHBFW_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFM9316869.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACHBFW_000529"
     CDS             complement(3654..4295)
                     /locus_tag="ACHBFW_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFM9316870.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="ACHBFW_000530"
     CDS             complement(4333..4647)
                     /locus_tag="ACHBFW_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316871.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACHBFW_000531"
     CDS             complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACHBFW_000531"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFM9316872.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACHBFW_000532"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACHBFW_000532"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFM9316873.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="ACHBFW_000533"
     CDS             complement(5984..6916)
                     /locus_tag="ACHBFW_000533"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFM9316874.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="ACHBFW_000534"
     CDS             complement(6913..7689)
                     /locus_tag="ACHBFW_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFM9316875.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACHBFW_000535"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACHBFW_000535"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFM9316876.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKVIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /locus_tag="ACHBFW_000536"
     CDS             complement(10933..12009)
                     /locus_tag="ACHBFW_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFM9316877.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="ACHBFW_000537"
     CDS             complement(12014..13294)
                     /locus_tag="ACHBFW_000537"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFM9316878.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="ACHBFW_000538"
     CDS             complement(13313..14239)
                     /locus_tag="ACHBFW_000538"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFM9316879.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="ACHBFW_000539"
     CDS             complement(14252..15535)
                     /locus_tag="ACHBFW_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFM9316880.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACHBFW_000540"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACHBFW_000540"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFM9316881.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="ACHBFW_000541"
     CDS             complement(16323..17234)
                     /locus_tag="ACHBFW_000541"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM9316882.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACHBFW_000542"
     CDS             complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACHBFW_000542"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFM9316883.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="ACHBFW_000543"
     CDS             complement(17700..18398)
                     /locus_tag="ACHBFW_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFM9316884.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="ACHBFW_000544"
     CDS             complement(18385..18771)
                     /locus_tag="ACHBFW_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316885.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="ACHBFW_000545"
     CDS             complement(18918..20588)
                     /locus_tag="ACHBFW_000545"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFM9316886.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="ACHBFW_000546"
     CDS             20771..21004
                     /locus_tag="ACHBFW_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9316887.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="ACHBFW_000547"
     CDS             complement(21103..21534)
                     /locus_tag="ACHBFW_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFM9316888.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="ACHBFW_000548"
     CDS             21613..22026
                     /locus_tag="ACHBFW_000548"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFM9316889.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="ACHBFW_000549"
     CDS             complement(22064..23212)
                     /locus_tag="ACHBFW_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM9316890.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="ACHBFW_000550"
     CDS             complement(23334..23930)
                     /locus_tag="ACHBFW_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9316891.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACHBFW_000551"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACHBFW_000551"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFM9316892.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="ACHBFW_000552"
     CDS             27160..27591
                     /locus_tag="ACHBFW_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9316893.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="ACHBFW_000553"
     CDS             complement(27636..28985)
                     /locus_tag="ACHBFW_000553"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM9316894.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="ACHBFW_000554"
     CDS             complement(29055..30218)
                     /locus_tag="ACHBFW_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM9316895.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="ACHBFW_000555"
     CDS             30347..30670
                     /locus_tag="ACHBFW_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316896.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="ACHBFW_000556"
     CDS             complement(30853..32202)
                     /locus_tag="ACHBFW_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM9316897.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="ACHBFW_000557"
     CDS             complement(32271..33224)
                     /locus_tag="ACHBFW_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFM9316898.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="ACHBFW_000558"
     CDS             complement(33326..34516)
                     /locus_tag="ACHBFW_000558"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFM9316899.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9896 a         7615 c         6102 g        11182 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggatta ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatataacac aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaa
//
LOCUS       DAYYME010000014        34663 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000014 DAYYME010000000
VERSION     DAYYME010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.014"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            591..1280
                     /locus_tag="ACHBFW_000559"
     CDS             591..1280
                     /locus_tag="ACHBFW_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9316900.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ACHBFW_000560"
     CDS             1267..2475
                     /locus_tag="ACHBFW_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM9316901.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ACHBFW_000561"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ACHBFW_000561"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFM9316902.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACHBFW_000562"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACHBFW_000562"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFM9316903.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ACHBFW_000563"
     CDS             complement(6578..7273)
                     /locus_tag="ACHBFW_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFM9316904.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACHBFW_000564"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACHBFW_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFM9316905.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ACHBFW_000565"
     CDS             complement(8472..8663)
                     /locus_tag="ACHBFW_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFM9316906.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ACHBFW_000566"
     CDS             complement(8684..9574)
                     /locus_tag="ACHBFW_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFM9316907.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ACHBFW_000567"
     CDS             complement(9561..10328)
                     /locus_tag="ACHBFW_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM9316908.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACHBFW_000568"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACHBFW_000568"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFM9316909.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACHBFW_000569"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACHBFW_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFM9316910.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACHBFW_000570"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACHBFW_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFM9316911.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ACHBFW_000571"
     CDS             14899..15282
                     /locus_tag="ACHBFW_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316912.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACHBFW_000572"
     CDS             complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACHBFW_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFM9316913.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ACHBFW_000573"
     CDS             complement(16226..17044)
                     /locus_tag="ACHBFW_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFM9316914.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACHBFW_000574"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACHBFW_000574"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM9316915.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACHBFW_000575"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACHBFW_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFM9316916.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="ACHBFW_000576"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="ACHBFW_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFM9316917.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="ACHBFW_000577"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="ACHBFW_000577"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFM9316918.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="ACHBFW_000578"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="ACHBFW_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFM9316919.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="ACHBFW_000579"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="ACHBFW_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFM9316920.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="ACHBFW_000580"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="ACHBFW_000580"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFM9316921.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="ACHBFW_000581"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="ACHBFW_000581"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFM9316922.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /gene="rpsF"
                     /locus_tag="ACHBFW_000582"
     CDS             27086..27385
                     /gene="rpsF"
                     /locus_tag="ACHBFW_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFM9316923.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="ACHBFW_000583"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="ACHBFW_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM9316924.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="ACHBFW_000584"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="ACHBFW_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFM9316925.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="ACHBFW_000585"
     CDS             28352..30325
                     /locus_tag="ACHBFW_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFM9316926.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="ACHBFW_000586"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="ACHBFW_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFM9316927.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="ACHBFW_000587"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="ACHBFW_000587"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFM9316928.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="ACHBFW_000588"
     CDS             33204..34496
                     /locus_tag="ACHBFW_000588"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFM9316929.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10565 a         6735 c         6854 g        10509 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagat caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgtaaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggatcaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggtggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccggc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagacatt gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atggggaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatcc
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaatcct tgaaaactat
    25441 atcgaacatc aacgcgaagt catcacacga cggacggtct ttgaaaaaga aaaagcagaa
    25501 gcaagagcac acatcttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaggcaaaag caacaatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgtacagc caaaggaatc ccaatcatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggtcagagg gatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aaaatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaataa ccatcaagac taagtgtgta agctagcaag
    26881 aataaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataattag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattagatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaat gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atattgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaagagg tagtgaaaga gacgtttagc cttattgccg ataggctgtc
    34621 tcttgaacac tgtttattca gaataaggga ctgtgacaaa aac
//
LOCUS       DAYYME010000015        33876 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000015 DAYYME010000000
VERSION     DAYYME010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.015"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..1026
                     /locus_tag="ACHBFW_000589"
     CDS             61..1026
                     /locus_tag="ACHBFW_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM9316930.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(1083..1313)
                     /locus_tag="ACHBFW_000590"
     CDS             complement(1083..1313)
                     /locus_tag="ACHBFW_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFM9316931.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1399..2286)
                     /locus_tag="ACHBFW_000591"
     CDS             complement(1399..2286)
                     /locus_tag="ACHBFW_000591"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM9316932.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(2375..3598)
                     /locus_tag="ACHBFW_000592"
     CDS             complement(2375..3598)
                     /locus_tag="ACHBFW_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM9316933.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(3615..4661)
                     /locus_tag="ACHBFW_000593"
     CDS             complement(3615..4661)
                     /locus_tag="ACHBFW_000593"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM9316934.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(4825..6552)
                     /gene="ptsP"
                     /locus_tag="ACHBFW_000594"
     CDS             complement(4825..6552)
                     /gene="ptsP"
                     /locus_tag="ACHBFW_000594"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFM9316935.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(6552..6818)
                     /locus_tag="ACHBFW_000595"
     CDS             complement(6552..6818)
                     /locus_tag="ACHBFW_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFM9316936.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            7297..9531
                     /locus_tag="ACHBFW_000596"
     CDS             7297..9531
                     /locus_tag="ACHBFW_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9316937.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            9679..9999
                     /locus_tag="ACHBFW_000597"
     CDS             9679..9999
                     /locus_tag="ACHBFW_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM9316938.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(10204..10428)
                     /locus_tag="ACHBFW_000598"
     CDS             complement(10204..10428)
                     /locus_tag="ACHBFW_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFM9316939.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(10626..12206)
                     /locus_tag="ACHBFW_000599"
     CDS             complement(10626..12206)
                     /locus_tag="ACHBFW_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFM9316940.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(12607..13983)
                     /locus_tag="ACHBFW_000600"
     CDS             complement(12607..13983)
                     /locus_tag="ACHBFW_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM9316941.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(14111..15199)
                     /locus_tag="ACHBFW_000601"
     CDS             complement(14111..15199)
                     /locus_tag="ACHBFW_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9316942.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            15371..15805
                     /locus_tag="ACHBFW_000602"
     CDS             15371..15805
                     /locus_tag="ACHBFW_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9316943.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(15883..16416)
                     /locus_tag="ACHBFW_000603"
     CDS             complement(15883..16416)
                     /locus_tag="ACHBFW_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFM9316944.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(16510..17688)
                     /gene="mutY"
                     /locus_tag="ACHBFW_000604"
     CDS             complement(16510..17688)
                     /gene="mutY"
                     /locus_tag="ACHBFW_000604"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFM9316945.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(17681..18478)
                     /gene="recX"
                     /locus_tag="ACHBFW_000605"
     CDS             complement(17681..18478)
                     /gene="recX"
                     /locus_tag="ACHBFW_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFM9316946.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            18585..19967
                     /gene="rlmD"
                     /locus_tag="ACHBFW_000606"
     CDS             18585..19967
                     /gene="rlmD"
                     /locus_tag="ACHBFW_000606"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM9316947.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(20141..20719)
                     /locus_tag="ACHBFW_000607"
     CDS             complement(20141..20719)
                     /locus_tag="ACHBFW_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFM9316948.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            21041..21511
                     /locus_tag="ACHBFW_000608"
     CDS             21041..21511
                     /locus_tag="ACHBFW_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFM9316949.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(21619..22845)
                     /locus_tag="ACHBFW_000609"
     CDS             complement(21619..22845)
                     /locus_tag="ACHBFW_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM9316950.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(23599..23799)
                     /locus_tag="ACHBFW_000610"
     CDS             complement(23599..23799)
                     /locus_tag="ACHBFW_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9316951.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(23933..24541)
                     /locus_tag="ACHBFW_000611"
     CDS             complement(23933..24541)
                     /locus_tag="ACHBFW_000611"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFM9316952.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(24626..25096)
                     /locus_tag="ACHBFW_000612"
     CDS             complement(24626..25096)
                     /locus_tag="ACHBFW_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFM9316953.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(25144..26058)
                     /locus_tag="ACHBFW_000613"
     CDS             complement(25144..26058)
                     /locus_tag="ACHBFW_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM9316954.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            26204..27007
                     /locus_tag="ACHBFW_000614"
     CDS             26204..27007
                     /locus_tag="ACHBFW_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFM9316955.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            27185..28132
                     /locus_tag="ACHBFW_000615"
     CDS             27185..28132
                     /locus_tag="ACHBFW_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFM9316956.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(28200..28493)
                     /locus_tag="ACHBFW_000616"
     CDS             complement(28200..28493)
                     /locus_tag="ACHBFW_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFM9316957.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYNPNLEEPTYHFKKQ"
     gene            complement(28521..29186)
                     /locus_tag="ACHBFW_000617"
     CDS             complement(28521..29186)
                     /locus_tag="ACHBFW_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFM9316958.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(29326..29958)
                     /locus_tag="ACHBFW_000618"
     CDS             complement(29326..29958)
                     /locus_tag="ACHBFW_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9316959.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(29965..31032)
                     /gene="liaS"
                     /locus_tag="ACHBFW_000619"
     CDS             complement(29965..31032)
                     /gene="liaS"
                     /locus_tag="ACHBFW_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFM9316960.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(31029..31760)
                     /gene="liaF"
                     /locus_tag="ACHBFW_000620"
     CDS             complement(31029..31760)
                     /gene="liaF"
                     /locus_tag="ACHBFW_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFM9316961.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(31929..32408)
                     /gene="greA"
                     /locus_tag="ACHBFW_000621"
     CDS             complement(31929..32408)
                     /gene="greA"
                     /locus_tag="ACHBFW_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFM9316962.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(32544..33719)
                     /gene="mltG"
                     /locus_tag="ACHBFW_000622"
     CDS             complement(32544..33719)
                     /gene="mltG"
                     /locus_tag="ACHBFW_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFM9316963.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT        10115 a         6865 c         5611 g        11285 t
ORIGIN      
        1 cttgtccatt atttcaaaat ttgttatagt ctttttattc aaagcgtaaa aggagatcct
       61 atgttaaaag aaaaaatgaa aactctcccc atgctgccgg gtgtctatct tatgaaagac
      121 cgagatggta aggtcatcta tgttggtaaa gcaaaacggc tcaaaaatag agtaagcagc
      181 tattttcatc agaataaaca gcattcaaaa aaagtattac gtatgattca tcatataaca
      241 gattttgatt tcgtcgtagt agacacggaa ttagatgctt tattgctcga gtgtcaattg
      301 atccaacatt accgtccttt ttataaccga caaatgaatt atttcagtaa ttataattat
      361 gtacatatta ccaataaggg atttgtatta acagatactc ctacggctcg aacatacggg
      421 ccttttcgtc tctataaaaa aatgccgtct attttgcgta taatggaaga aacttatcaa
      481 atgccttggt tgtctgaaat tagtttgtta gctttgcgag tccagcttcc tgacctacag
      541 gaaatgactt ttgaacagaa aaaaaaggaa ctccaagggc tgtttcaagg ccgaaataaa
      601 aagctactga cttacttgaa aaaaagacaa caacatttca tctatcagtt gaatttcgaa
      661 aaagccggta tgcttcaaaa agatatcgaa ttagttactt attttattag acggatccag
      721 gaacaaaagc agtttttaag aacaccatcg ctcaccttca gcatgccatt agcagcagat
      781 gagagtcaga aaaagcacta cctcgtttgt tatggtcaac ttgcagaaac aatgattacc
      841 tctggtgacg tttctcctga cttctattat gaaaagaaag aagctcattt atctttgaaa
      901 cgtcaattgt caaaagaaga aatcgatcct gtacaaatct tgatcagtta ccgcaaaaaa
      961 ttagaaaaag aacagtttga aattgaactg ttaaacaaaa aagaagctga aaagcaacta
     1021 aattagttgc ttctcggctt cttttaggca tcttcaactt caaatgtcaa ttatcctaaa
     1081 atttattgga ttaagtcata gatttccatc gctacgatat cgatattatc aaattcgtat
     1141 tgctgatgag tatctgtttc agttaattca aatgtatcgg tcgctttatc ataagttact
     1201 gtacacttct ctacgccttc tttttcaaaa cggcgaactt gcagttcatt atctgctgac
     1261 tcgaccatag cctccagtcg ttttatgatt gcgactaatt gtgaatgtgt catcggaggt
     1321 cctccttttc ttatttctgt tatattgtat caaaagcata gcctacttgc attgattttc
     1381 tctaaaaaaa ctcgtttttt atgatttttt ttctggtatt tttccttcag gccaccaaag
     1441 tttgatcaaa gcttgggttc cgtcattctc aaaaccttta tctgccaact cttcatatag
     1501 tttcaatgct tgggtagttg ctggcaatac aagatccatt ttttctgctt cttctaatgc
     1561 aatctttaaa tctttgataa aatgtttgac aaaaaaacca ggtgtgtaat cttcttttaa
     1621 aatacgagga gaataattgc ttaaagacca gttagctgca cttccacctg aaagcgtctc
     1681 aatcactttc tttaaatcaa gtccgttttt ttgtgcatag acaagcattt ccgtcattcc
     1741 tgtcatcgta cctgcaatca tgatctgatt agccattttt gtatgctgac ctttcccagc
     1801 agaaccatgc aatgtaaatg tcgtaccaaa ttctttgaat aaaggcagta cttgatcata
     1861 aacggcttct tctccaccga ccatgatcgt cagtgtacca ttctttgctc ctaagtcacc
     1921 accggatacc ggtgcatcta acgcatctgc gcctttttct tttgctgttt ttgcaatttt
     1981 ttctgccagg cttggtgtac ttgtagtcaa atcaactaaa atatgtcctg agatatctgc
     2041 agaaaaaatc cccgactgtc catagtagat ttcttctaca tctttggggt aacctaccat
     2101 tgtaaagaga atattacttt gttctgctac cgcttttggt gtatctgccc agatagctcc
     2161 ttctgttact aaaggatcag cttttgcttt tgtccgatta tagacagcta cttcgtgtcc
     2221 tgcttctagt agatggcgtg cgacagcact tcccatgacg cctgttccga taaaaccaag
     2281 tttcattttt tcgacctcct aaaaatcaaa aatccttgct tctatgctag tatacgctag
     2341 aaacaaggat tttttcataa aaaatgtttg aattttatct tttaaatgaa tttaatttta
     2401 ttttgattcg ttcaatcgag tcttcttctt cttttcttcg catctcttct tcatgataaa
     2461 caaccatgtc ttgataaaat tcgatcatag cttcgccgaa atgtgtagat gaaatatcat
     2521 aaagtttagc ttctaaaact tttggatctt taggaacttc actggaaata taatcaataa
     2581 gtgctggtgc aaagtcttcg tcggtttcaa aagttttccc taatgaagga tgatcgaaca
     2641 atcggttcaa ataagcattt ccttccacga cacaaggtgt tccggctgcc atcgcttctg
     2701 tataagtaag cccttgtgtt tctgaagtag aagcactgac aaaataatct gccgctttat
     2761 aataaagtgc tacatcattg ttgtccactt ctcctgtgaa ctgcacaaat tcttctaact
     2821 gcagttctga gatcaattcc tctaactctt ctacataagg tccttttcct acgataacta
     2881 atctggcgtt aggataagcg gagatgacct gaggaaaccc attaatcaag gcttggatat
     2941 ttttctcata agaaatcctg cttaaagaaa gaatcatcaa atggttttcc tgaagaccta
     3001 gtgactccct taatttgctg atatcctgct gagtgatatc agaacgtttg aatttttcaa
     3061 tgtcgatacc agtaggaatc acgcgaagcg gtgctattac accatagctg cgtaatgtat
     3121 caataacacg ttcacttgga caaactacac ctgtcgtatg atttgcaaat aatctagaca
     3181 agtattttac atgggattgc ctgatcactt ttcctttacc gatataatga aggtagtctt
     3241 catacatcgt atggtaagta tggatcagag gtattttcaa cttcttagct accatcttcc
     3301 caagtatccc tgctccgaat tctgtatggg tatgaataag atctagttct agttctttgg
     3361 caaccaaata agcataccac atgcctctaa caacaactct tcgatccttg aaagaaataa
     3421 atggtacact tggcattcga atcacatctt tttccaactt atccgcatta ggatcggtcg
     3481 ttgtaaaaat atagacttca tgcccatgtc tttctaattc ttgttttaat gttttgatcg
     3541 atgtagcaac tccgctgact tgcggaaagt aggtatcggt aaaaaagccg attttcatgt
     3601 ttttcgcctc caaactagtc cttgacctga tttacttttt ttaatttgct agagactttt
     3661 tcattcagta cactttcgta tacattttta agttcttcac cgattttatt gatacttcgc
     3721 tcttttgctg tttgataacc ggcttgtgtc gtactcggta gttcttgatt ggtaatctgt
     3781 tggatcagtc ggataaattc ttcgtttgtc ttccccatat agcagttctt gctatcttct
     3841 aaccagccat tatacactgg aatatctcga acaagtacgt tttgtttgct tgctaaagct
     3901 tctaaaacaa cgatcccttc tgtctcttca taagaaggga aaaagaatag atctgcacct
     3961 gaatacgcac cttctatgat ctctccacga atgtaaccag gaaacaggac attttcagga
     4021 tgatcttctt ttacaatttt ccggatgttc cgaggaatcg agtacatggg aacatgacca
     4081 aaccaaataa atgtgtattc gggcatcttc ttagcaattt ctacgaaatc aatgattcct
     4141 ttacgttcga aaaacaaacc gacacaaatg attacttttt gatccggaga cagtttgaaa
     4201 tattctctga acttttgttc tttcattgga tcagggtgat atttctctaa gtcgatccca
     4261 ttggaaatag cttgaatcgg cacacggata ccatagcttt ccaacaactg ttttgaataa
     4321 ggtgttggcg tgatcagata gtccgctttt tggtacaatc ctaccagata tttttttacg
     4381 ataggagaaa gctggttcga tctgataaag gaatttcgga aatcttcctc tgtagaatgc
     4441 gcatgataga caactttttt tccttctctt ctggctttat tgaccatatt atgactattg
     4501 attccgtatg tattgatgtg caggatgtca taatcttctt tttcatctag tgtataagag
     4561 ataccgactt ccgtcagagc acgtttttga tgatcaagcg cacgcccgat tccagatttt
     4621 gcaagcatct tttcactttc aaaatataat aataccttca ccttacgtcc cccttcactt
     4681 atatagtata acatagccta caaactgtca ctcaaactta agccctattt ttgtctattc
     4741 ttactgacaa aaacgttttc ttaaaaaaga acaaaaaaac tggcgtagct cttttacaag
     4801 ccacaccagc ttttgatacg ttgtttattt tacatattca tgaacaagtt caacgacttc
     4861 ttccatcata tcgcattctt tcaatgcacg atcagcaagt tcagccattt tcgctgtgtc
     4921 caagcgtttc attaaactac gcgttttaag aatagatgtc gcactcattg agaactcatc
     4981 tagacccata cctactagca atggaacagc tgtttgatct ccagccattt ctccacacat
     5041 acctgcccat tttccttcag cgtgtgctgc atcaattaca tttttgatca aacgtaaaat
     5101 tgatgggtta tatggttggt acaagtaaga aacgcgttcg ttcatacggt ctgctgccat
     5161 tgtgtattga atcaaatcgt tcgtacctac agagaagaaa tctacttctt ttgcaaactt
     5221 gtctgctaag actgctgctg ctggaatctc gatcatgatg ccgacttgga tcgtatcaga
     5281 tacttccttg ccttcactta tcaatttttg tttttcttct tcaaagatcg cttttgctgc
     5341 gcggaattct ttgagtgtag ctaccatagg gaacatgata cgtaaattac catgaacaga
     5401 tgcacgaagc aatgcacgca tttgtgtacg gaacatttcg tcgccttgtt cagataaact
     5461 gatacgcaat gcacgatatc ctaagaatgg attcatttca tgaggtaatt gcagatatgg
     5521 tagttcttta tctccaccga tgtccattgt acgaacaact acaggttttc cttccatacc
     5581 ttctaataca gctttgtaag cttcatattg gtcatcttct gttgggaagt ctggagaatc
     5641 catatacagg aattcagtac ggtacagacc aactgcttct ccaccattat tatggacacc
     5701 aactagatct tttggtgttc cgatgtttgc agctaattca aaatgcttgc cgtcagctgt
     5761 gacagtttct gcatttttta atttttccca ttctgctttt tgtgctgcgt aatctgcacc
     5821 tgctttttca aattcagctt tttgttcatc tgtaggatcg atgattacat cgccttcgat
     5881 cccgttgaca gccaaaatat cgccttcttt gactttagca gtaatttctt ttgtcccaac
     5941 gattgctgga atttctaaag aacgtgccat gatagcagaa tgtgaagtac gtccaccaat
     6001 gtctgttaca aacgctttta caaaattgcg atctaattgt gctgtatcac ttggagtcaa
     6061 gtcatgtgca acaacgacca cttcttcgtt gatcattgat ggatttggta aagtaacgcc
     6121 taataagtgc gctaaaatac gtttagcaac gtcacgaata tctgcagcac gctcttgcat
     6181 ataagcatta tcttccattg cttcgaacat accgatatac atatccgtta cttcttttag
     6241 cgctgattca gcatttacgc tgttgtcttt aatattttgc ttgatttgac ctaccatttc
     6301 tggatctgaa agaaccatca aatgggcatc aaatacttgc gcttctgctt cacctaagct
     6361 ttgcgctgct ttttcacgaa tttgctgcaa ttcttcagta gatttagcta atgcagcatc
     6421 caaacgagct tcttcagccg cagtatcttc tactgttgcc ttactgaatg ttaaatccgg
     6481 ttgaactagc aggtaagctt ttgcaacggc tacaccgtca ctagcggcaa tcccttttag
     6541 catttcaacc attattcaga taagccttct ttcttcattg tttcaacgat tgctgccatt
     6601 gcatcagctt cgtcagcacc ttctgcagta atagtaacat cagaaccttg acctacacct
     6661 aaagacataa cgcccatgat tgatttcaag tttactgatt tacctttata ctctaagttt
     6721 acatcagagt taaatttgct tgctgtttgt actaatagtg tagctggacg agcgtggatc
     6781 ccagtttctg ctactacgtg aaattctttc ttttccatat taatcggtct cctttaagtg
     6841 agtgttgttt ttatgtgagg gttttctttt ttagtaattc agagaatcct aacaaatgcc
     6901 accctttcat ctgtaaagat taacattttt tttcgttaga tacaacagtt ttcattcaat
     6961 ttcagataaa agtattcaaa ttattccgga ttctttttca tccttttctt cacattttat
     7021 aacaaacgtg tttatttcct ttttcttgtc gctttctctt ctcctgacgt ttatcattct
     7081 ttcctttgac cctctttgtt ttcatatgaa acgaattact tgcttccctt tttattttta
     7141 gtataataag agtcaaaggt caggaatggt caatagagat ttcacctttt ctacctcata
     7201 aatctcttaa aaaactaggg gttattcttt tacctcatga aaaacgagat gtaaaatatg
     7261 gatgtaccca tttttgtgaa aggacgtgaa tgattcatgc tttgtcaaaa ctgcggcaag
     7321 aatgaggcaa cgattcactt gtatgccact gtcaatggtc agcggacaca actcgattac
     7381 tgtcagagct gttatcaaaa aatcaaaaat caacaaaatg gaggtctgac taatatggcc
     7441 caaaatgatc catttggatt tggcagtttg gacgatcttt tccgttccat gtctcgccaa
     7501 atgcaacagc aaggaaatta tgagcaaaca ccaccaacac aatttggcgg aaacaacaac
     7561 ttcaatggtg gtcagccacc tcaaggcaat acagaaggtt tattgggaga atacggaatc
     7621 aatatcacag aacaagcacg acaaggtgat atcgatcctg tgatcggacg cgatgacgaa
     7681 atcaaacgag tcattgaaat tttaaatcgc cgaaccaaga acaatcctgt cttgattgga
     7741 gaaccaggtg ttggtaaaac agcagttgtt gaaggtttag cacaaaaaat cgtagatggt
     7801 gatgtaccgc aaaaattaat ggacaaagaa gtcattcgtt tagatgtcgt atctttagtc
     7861 caaggtactg gtatacgagg acaatttgaa gaacgtatgc aaaaattgat tgaagagatc
     7921 cgtcaagcag aaaatgtgat cctattcata gatgaagttc acgaaatcgt cggtgctggt
     7981 tctgctggtg atggaaatat ggatgctggt aatattttaa aaccagcttt agctcgagga
     8041 gaattgcaaa tggttggtgc tactacctta aatgagtacc gcatcatcga aaaagacgct
     8101 gctttagaac gtcgtatgca acctgtacgg gtagatgaac caactgtcga agaaacgatc
     8161 agtattttga aaggtttgca aaaacgttat gaagactatc atcatgtaaa atatactgat
     8221 gaggccatcg aagcagccgc aactttatcc aatcgttacg tccaagatcg cttcttgccg
     8281 gataaagcca tcgatttgct agatgaaaca ggttctaaga aaaacttgac gatccaaatc
     8341 gttgatccta aaacaatcga gaaaaaactg caagaagcag aagaacaaaa agttttagct
     8401 tctcgagaag aagatttcga aaaagcagct tattatcgcg atcagatcaa taaacttcaa
     8461 aaaatgaaag aacgtcagct cactgaagag gaaacacctg tgattaccga aaaagatatg
     8521 gaaaaaatcg ttgaacaacg gacaggtatt ccagtgggtg aattaaaaga aaaagaacag
     8581 acacaactga aaaacttagc agatgacttg aaagctcatg tcatcggaca agataacgct
     8641 gtagatcgag tagcgaaagc gattcgccga aaccgtgttg gattaaacaa acagaatcgt
     8701 ccaatcggct ccttcttatt tgttggacca actggtgtag gtaaaacaga gcttgcgaaa
     8761 caacttgcct acgaactatt cggttcacaa gattcaatga ttcgtttcga tatgtctgaa
     8821 tatatggaaa aacatagtgt ctctaaattg attggttctc ctccaggtta tgtcggatac
     8881 gaagaagctg gtcaattaac agaaaaagta cggagaaatc cttacagtct tgtcttgtta
     8941 gatgaagtag aaaaagctca tccagatgtt ttgcatatgt tcttgcaaat cttagatgat
     9001 ggtcgattga cagatgcaca aggaagaacg gtcagcttta aagatacgat catcattatg
     9061 acaagtaatg ccggaactgg taaagtagaa gcaaacgttg gttttggtgc ggcacgcgaa
     9121 ggtgttactc gttctgtatt gaaccaatta aacaactact ttacaccaga atttctgaac
     9181 cgttttgacg ggatcatcga attcagtgca ttatcaaaag aaaacttgat gacaatcgtc
     9241 acactaatgc tagacgatgt caaccaaatg ctggcagcac agcaacttca catcgaagtt
     9301 cctacgaatg tcaaagaaaa attagttgac ttaggctatg atccatctat gggtgctcgt
     9361 cctcttcgac gcacgatcca agaacaaatc gaagatggta tcgctgaatt ttatcttgat
     9421 cacccttcta tccatgagtt gaaagcaaaa ctggataaag atggaaaaat catcgttact
     9481 tctaaaccag aacgtttggc taaagaatca gccgaagaaa cggctgaata aatccagttt
     9541 tatagcgtga gacggtattt ctttgagaaa tgccgtctca ttcttttttc agaaaagtca
     9601 aaatactgtt cattttataa aaaagaaatt ataataagag aaacaagcaa ttaaaaatac
     9661 ataaaagagg tgagcgccat gaaattggta aatgtaacaa acagccattc ccgtcttgta
     9721 ttaaaccagc ttgaaaacac tgatgcccat ctagtcaagg tctacacagc cggaaatacc
     9781 actattgtct acactgaagc ccctgaacat aatgaaattc tgctgatcaa tgacaaacgt
     9841 aaaatccagc cgaaagaaat cgaagatgct aaaaattatt tcttagataa aatcaaagac
     9901 gccgtttacc acgaggatga aatcaaagta atcgaattgg caggccttgt tgaaatctcg
     9961 attccaaaac atgaattagg cgctacatta gccgtgtaaa caaattttga acataataat
    10021 taaagagcgt gggacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac
    10081 ggcgaaagca gaagcaactc cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa
    10141 atttttgtga attccagctt atttcttgga gttaaacgct tctgtctcag cctctttttc
    10201 tttctagctt tttgattttt ctcttttctt ttgtactttt ctcttttcat tttcagttct
    10261 acgcgtgtct ttatttttaa ctttttcctc tttatggatg attgatcgta ctttttgtgc
    10321 aatttgctcg tttttgtccc agtcttttac atctaataat cgacataatc gtaacatctc
    10381 ttcttttttc tgtttttttc tttcacgtct ttgccattct gtttccatgt gtcacactct
    10441 cctttcttta atcatatgta cgcaaaataa tttttatatt tttttgatta gacatttcta
    10501 tgattttaat atagtaaaaa gagccgtcac ggaaaatccg tcacggctct aaacaaaata
    10561 tgagaggctg tttttgtttg gctattctta ttacttgatg cagggcagct tgtgtcaaga
    10621 cttctttaca tcaaactttt taattttaca ttgggatatt tatccgcaaa ccagcgttct
    10681 gcaaattggt tctcgaataa gaacaacggt tggtcgaaac ggtcacgagc caagatattg
    10741 cgacttgaac tcatcttctc atccaaatct tctggatcga tccagcgagc gattttgtgt
    10801 cccattggag acatgattac ttcggcgtta tactcattta acatacgata ttggaatact
    10861 tcgaattgta gctgaccaac agctccaatg atatattctt ccgttagata tgttttataa
    10921 agctgaatcg ctccttcttg aactaattga tagattcctt tgtggaagga tttttgtttc
    10981 atgacatttt ttgcagtcac tttcataaac agctctggag taaatgaagg gagttcttca
    11041 taagcaactt ttagttttcc ttcgaacaat gtatccccaa tctgataatt gcctgtatca
    11101 tatacaccga tgatatcgcc tgctactgct ttttcgacgt tttctctcga atctgccata
    11161 aattgtgtga cattgcttaa tttcagtttt ttatttgtac gttccaacca gacatccatt
    11221 cctcgttcga atgttccaga acagattcgg acaaaagcga ttcggtcacg atgggctgga
    11281 ttcatatttg cttggatctt gaatacgaaa cctgaaaatt cttcctcata aggacttacc
    11341 atctgatcat cttgtgtttt gtgtccataa ggagctggtg ccaattcaac gaaagtctct
    11401 aaaaaagtct gtacaccgaa gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg
    11461 ccacgagcaa ttttttcttg attaaatgca tctccagcct ctttgattag ttctacttct
    11521 tctaagactt gttcatataa ggaattttca gttagaggat gattttcggg aagcagtcca
    11581 tcttcatcca attctactaa tcgttcatcc tgataatttt ccggacgata aaactctaca
    11641 cgattatggt agatatcata aagaccttct agccctttac ccataccgat tggccaattc
    11701 atcggataag attcgatatc taatagctct tctaattctt ctaacaattc caatggttca
    11761 cgaccatcac gatctagttt gttgataaaa gtaaatatcg ggatgccacg tttttttaca
    11821 acttggaata atttctttgt ctgtgcttcg atcccttttg cactatcgat taccatgacc
    11881 gcactatcca cggccatcaa agtacgataa gtatcttcag aaaaatcttc atgtcctggt
    11941 gtatccaaga tatttactcg tttcccttga taatcaaact gcataacaga actagtaaca
    12001 gaaatcccac gttgtttctc gatttccatc cagtctgatt tggcaaaatt acctgttttt
    12061 ttgcctttta cggtcccggc ttgacggata gccccaccaa acaatagtaa ttgttctgta
    12121 atcgttgttt tcccagcatc cggatgggaa atgatcgcaa aagttctgcg attgtccact
    12181 tgttgcttta attttggatt attcataacc taactccatt tcttccattc aattatttat
    12241 attttctcta ggcacagatt gtccgatctc ttaaagaaag acaatttcat atagggctgt
    12301 gacaaaagcc gtgtagcagt ctctttttcc gagtaaacgg cgttctagag acagcctatc
    12361 agcaataagc ccacccaagc caaaaatacg aaaagctgtt tttgcttggc tgctcttatt
    12421 acttaatgca ggatagcttt ttcacaacct ttgtcaacta ctacaactga atcagtatag
    12481 catggattca gcgaatgaaa aagccaccaa accataagag tcaaatattt ttttgtcagc
    12541 tgactaaaag aaaagacgtg ttgcttgttt attcaagcaa cacgtctttc ataaccaagt
    12601 ttccaattat cttcttggct cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc
    12661 ttcaggttcc gcttctgttt cttccggatc atgaaaaatg acacggattt taaggattcg
    12721 agaaccttcc atttcttcag aaaccagcgt aatattatcg acatcaaacg ataacttttc
    12781 gccttcatcc ggaatcatgc ctaatgctgt aatcaaataa ccagccatcg tatctacatc
    12841 actcatatgc agatcactgt caaaagcttc attgaactca tcaatcagca tacgtccttg
    12901 gatgatatat tcgtgttcat cgattttttc ataaagattc tcaacttcat ctgattcatc
    12961 atcgatttct ccgacgattt cttctaaaag gtcttctagt gttgctaacc caacgacgcc
    13021 gccatattca tctaataaaa tagccatctg attctgcgtc tttttcattt catataacag
    13081 atcatcaata aagaccgttt caggaacgaa taaaggctcc tgcatgattt tcttgatatc
    13141 taaattatca aagccgaatt tatgggcagc ttttagtaaa ttctttgtat gaaggattcc
    13201 gactacttta tctttgtctt cgttataaac agggattctt gaatagtttt cggataaaac
    13261 ttcattcaca ttttcttcca cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg
    13321 gaccatgact tctcgggcaa ctttcgtatc aagagaaaaa accccttgga gcatttctaa
    13381 ttcttcatta ttcaatacgc cttcgttttc aagcatataa cgcatttcat ctctggtcat
    13441 ttttgaatct tcatcatcaa aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc
    13501 agaaagcagc cagacgaagg gcttagctac tacaccaatc aagcgtatca gccctgaagt
    13561 gaactttgcg acttcttctg atttatttaa agcaatacgt ttagggtaca attctccaaa
    13621 aacaatcgat acataggtca aaatagccag cacaatgata tttgcaatat tttttgccgc
    13681 tgtgccgcca cctaataccg gagctaaccg ttccgataaa gtattagcta gtgaagcacc
    13741 agacaaaatg ttgaccaaag tgattcctac ttggattgtc gataggaaat tatttggatt
    13801 ctgcaatatt tttaaaagct tttgagattt cacatctcct tcatcagctt tttgctcgat
    13861 tcgattctta ttgatcgaaa caacagcaat ttcggaagct gctaaaaaag cattgatcaa
    13921 tgtcaaaacg atcaataata aaatctgcgc taacagcgac tgactctcag ggtcagcatt
    13981 cataatcgca ttctctcctt ataaaattaa tttttagttt acgatagaaa aaaacgtggc
    14041 actatagcca cgttaaaatg gtatcacaaa gcaattttct ttgcaataaa aacaagtgta
    14101 ctaaaactac ctacgaggtc gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt
    14161 tgacgatttt gattatatag gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta
    14221 agaagatacc aagcaatccg gcaatatttc cagctactag aagaacgata atgatcgtta
    14281 atggatggat tttcagtgat ttcccaatta cattaggata aatgatattc ccatccaact
    14341 gttggacgat tagcacaacc acacaacaaa gtaatgcttt taccggatca ttgaagactg
    14401 ttactagaaa tgcaggtgct agtcctagat aaggtcctaa atatggaatt aggtttgtca
    14461 atcccgcaat gaccccaaat aagaaagcat aatccacacc aattaccata tatcctaaaa
    14521 atgtgaaggt accaacgaat aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg
    14581 ttttattcag ttgccccaat aactccacaa tttggtttct tcttttttct gggaaaaact
    14641 gttggatgtt tggcactagt ttttctccgt ctttcaacat atagaacaag atgaatggtg
    14701 ctgtaatgat tactacggtt gccgaagcga tcgttccaac gatcgaacct aaactattgg
    14761 atactccact aaggaattgc tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat
    14821 atttattgat atctacttcc ttgaacaaag gcaattcggc tacctctttt aaccagcttt
    14881 ctacctgttt gatgaaatca ggcatattag atgccaatga ggaaatttgc tgtactagac
    14941 ttgggatgac actaagtaaa atccaaataa ttgctgcaac taaaaggagc agcacaattg
    15001 cgacagccca tattcttttg atttttgctt tcatcaacag cttgacaata ggattcaata
    15061 aatagtaaag aaagcctgca atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga
    15121 tcggttcgaa aataaagttt atttctgatg aaatcatgat caatgcagct atgatcaata
    15181 attcaagcga ccaaaacatt agtttagact gtctaagttt ttcaaacaat agatttcctc
    15241 ctttctttct tactagtata tattatattc gttaaaaatt cttttattct ttttttattt
    15301 ttgaaaactt gccatacttt ttatttgttt tattttccag tatactatac gaaaggaagg
    15361 tggctagacc atgaaagttg tacaaacaaa agatacaatg agcaatattt acttagatgc
    15421 tgtacggatc aggcatcaag tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat
    15481 agacaaagat gaagcccatt gtatccactt cgtcctgtac tcagataaaa aagaaccgca
    15541 aggaactgta cgtcttttgc ctttagaaaa cggaaaaatg aaattacagc ggatggcgat
    15601 tctttctgaa tacagacacc aagggttagg aaaaatattg attgaagaag cagaaaactt
    15661 tgctaagaat caaggctata atacgatttt attaggggca cagtccacag ctgaaacttt
    15721 ttacgaaaaa ctaggatata cagcttacgg cgatcctttt gaagatgctg gcatgcctca
    15781 tatttatatg aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct
    15841 tttgagaaaa atctctcact ggcttactgt tcttttttgt gtttacttac gcgacaattg
    15901 cttgtaacgg ttataccaaa tatcaatata gccttccgag aacgggccat ctccattatt
    15961 gatccagtca accagaatct tcacattttc ttttaggatg aagtcaatat cgttcgggta
    16021 attcatcatt ttgctgtgca tctcatattc atcgacatcc aatagtcgtt tctcgccgtc
    16081 aggaaatacc ttgatatcca agtcatagtc gatatatttc aaggcctcat cgtccagcaa
    16141 aaaaggtgaa gctagattac aataatagga aacccccttt tctctgatca ttgctattac
    16201 attgaaccag tattttttat gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg
    16261 tccatcagat tcagtgacta atgtgtgatc attcacgccg atcatagagt gttcgcttgt
    16321 ttttaatacc atggtatctc gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg
    16381 gatcgtcaca aactctcctt cttttggaac tcccatagca acccttcctt aagcagcatt
    16441 ttgtgctttg tgtttgggtg ttcattacac cgacatcata attattatat caataaaaat
    16501 gagctttgcc tagaaaggat tcctattgtt tcgatttttc tctaactgat ctaccagttt
    16561 catttggacc ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt
    16621 tttgttctcg gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac
    16681 atgccatttc agatgactaa aaatatgagt aacttctcct aaatgtcgtg tctgccaaac
    16741 aaagagttcc tgcttttcaa atgggagatt ttgctcttct tctgctacta ggtcatcaaa
    16801 cagatcgatt tctggctttg tttcccattc ttttttcaat cgctcatact cctcttgtgt
    16861 cacttccatc attggaaatg tccacatatt cgccagcaat ttttggctat ctctttcttc
    16921 aaaataatac gctccagaat gattttgaag agctgcactg atataataaa cgtcttttgg
    16981 ctttgctttt tttgttttta caggaaaact cgtttggatt cctcgtttgt tagctaaaca
    17041 aaaggcttgg atcggacaag tctcacactt tggagaagtt ggtgtacaga tagctgaacc
    17101 tagatccatc attgcttggt tgaattcacc tggatgcttt tcatcaatga ttttccgcat
    17161 cgcttcatca aagattttcc tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa
    17221 tctgctgact actcgcatca cgttgccgtc aactgcaggt tctggaagtc caaatgcgat
    17281 acttgcaatc gcgcctgtcg tatatggtcc tattcccttc aatgagctaa tttcttcagg
    17341 cgtttgcggc atttccccat caaattctga catgatctgt ttagcagcag cttggatgtt
    17401 ccgggctctt gagtaataac caaggccctc ccatgctttt aaaagctttt cttctggagc
    17461 atttgctagc tcttcaattg taggaaacca ttccatgaaa cgataaaaat aatcgatcac
    17521 tgtgtctact ctggtttgct gaagcatgat ctcagagatc cagatacgat aaggatcacg
    17581 attatagcgc caaggcaggt ttcttttttc ttgttcatac cattgaatga actgatcttg
    17641 aaattctttt gtttctttat ctgtccattt ttcccattct ttattcactt tcctcttcct
    17701 tttccgtaat gaatcgctgg atcatatcca aatcaaatcc tttttgatat aagctttgct
    17761 ttacttttag atttcttttt ttaggttcaa accttaaatt tcttcgccat agtttatctc
    17821 cttgtttgac taaataatca tattctattt cttgatctgc ttcttgcgga agatcagcta
    17881 tcgcttgttg gatcacttct tgagaaaagc ctttcgtcat caatgtttgt tggatttttc
    17941 tcaataattc tttttggctt tttccgtgat tttttccaaa cgctttttcg gctgtctgat
    18001 gtgcattttg aacttggtct tcaaatgtat attggctgag cgcttgttca atgatttcag
    18061 gtttcaaacc tttttgctgc atctgctgcg ctaatttctt tggtcctttg tcacttaagc
    18121 gcatattcgt gcgcacataa ctttccccat agaccaaatc atcgacaagg tccaattctt
    18181 ttaagcgggc tacgatttta tttcgatctt cttgaaggat ttccttttct tttaagtatg
    18241 ttcggatttc tttttccgat cgcaactgat aacttaagta attcattgct aggtgaaaac
    18301 cgaaatcagc tccgctactc ttcttgatct cttggatagt ttcttcgtct aattcccgtc
    18361 cttttaataa tcgatagcgt actaatacat cttcagatac ttgtaaagct tctccttcag
    18421 ataaatctac gcgatagaaa gggcctttgc ctttactgat tctcgtaacg gttaacataa
    18481 acatcctcct cgaacatata ttctctatca taccacagaa aatccccgta atcaggtatt
    18541 atatgttaga ataaaccaca gaaaaaagaa aaagggagat acttatggca gaaacaattg
    18601 tcaaaactgg acaaacggtt cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa
    18661 tcggttattt caaacggttg atcatatttg ttccatatgc actaccaaaa gaagaagtac
    18721 tagtgaaaat cacgaaagca actcctcgct atgcagaagg agaattaata aaagtcaaaa
    18781 aaacaagcaa agatcgcgta gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc
    18841 aattacaaca cttagcttat caagctcaat tggattttaa aaaggatctg cttttgcaag
    18901 cattggaaaa attcaaacca gcaggctttc gatcttatca acttttgccg acgatcggga
    18961 tggatgagcc ttggcattat agaaacaaag cacaatttca gttacgaaaa aataagcaga
    19021 ctcaaaaaat tgaagctggt ttatacgaag caaactctca tcagttagtc cctataacag
    19081 actgcttggt tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt caactattga
    19141 ataaatttga tgcacccatc tatgatgaac ggacaaacag tggaatcttc cgcacaatca
    19201 tggttagaat cggtgttaaa actggggagt tacaagttgt ttttattact cgcagtaaaa
    19261 aattcccgca aaaaaacgca atgatccgtg agatcaatga acgactgccc gaagttgttt
    19321 caatcatgca aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc
    19381 acttatgggg aaaagaagcc attgaagaac aaatcaatga agtgactttc gatctttctc
    19441 ctcgtgcctt ctttcaactc aatcctcagc agaccgaatt gctttatggc gaaggaatca
    19501 aagcattaga tgtccagcct aatgaaacga tcgtggatgc ttattgcgga gttgggacga
    19561 tcggcttaag tgtagcaaaa caagccaaag aagtacgggg aatggatacc gttcctcaag
    19621 caatcgctga tgcaaaaaaa aatgcggaaa gattaggata tcacaacact cgatatgaag
    19681 tcgggacagc agagcaagtt ctgcctaaat ggttgaatga aggttttcag cctgatggta
    19741 tcatcgtaga tccaccacgt acgggtttgg ataacgcatt gatccaatca ttattgaaat
    19801 accctccaaa aaaacttgtt tatatctcct gtaatgtttc tacacttgcc agagatctgg
    19861 ttaatttaag taaaaagttc aagattgaat atttacagtc cgtcgacatg tttcctcaga
    19921 ctgcacgttg tgaagttgtc gtgaagatga cgagactcgc tgactaaatg aaagaggttg
    19981 tgaaaaagtc tgccctgtat caagtaataa gaacagccaa acaaaaatag ctcttcatgt
    20041 tttatgaata ctgtacttgt gaccgaagag ttaacaggag gacactattt atttaaaaaa
    20101 agggttgtga catagttttt gtcacagccc tttttgatta ttattggata ttcacgtcta
    20161 tcacttgata aaatgcattt gtcgtatcgc tgatattcca tactgctaaa atcacattat
    20221 atccttttcg atcctgcgga agtgtgatcg tttggttgac tgtttttggt ggaacagttg
    20281 ctttgtcgtc tatttttgtg atcaattcaa aatcactaaa tttcaaaggt tgattaggat
    20341 tccaagttgg tttggtaatg tagtaatccc aagtacttgt ccgatgttgt gctgttaaat
    20401 tccaagtaat atttaatgtt cctggattaa ccatgttttt atgccatctg ttagatgtct
    20461 gttcatctaa aggctcaaag ccgctgactc ctgcactagc aatttttcca tcaatgaatg
    20521 tatttttggt agcttcgata ctttgcggtt cccattgtgc tcttccgaca ttttgattta
    20581 agtttcctgc tgctgatgtt cctaggtatg cacggcttcc tggagaagta atataaccat
    20641 gcgcaaaacc tggaatgcta aaaccgaaca accctaaact aactaaccca ataaacaata
    20701 acgtactaat ctttttcatg tagaaacctc cttcatttga taaccacatt gtaaacgctt
    20761 tattgtatta acacaaacat tatgctttta taattttatg ttgaagtaaa aaaggtgaaa
    20821 aaagtaagat gcagattata acagaagtag acgagaaaag tacaaataga aaacatgaga
    20881 atcaaatcat atgatctttt tcaaaaacat cattcatata aacaaatgtt caacttttta
    20941 ataacaacaa ttatcaattt ttttcttact tcacaaacta aatacaggtc ttgctgtgag
    21001 ccttcatggt aaactaatat tatagaaagg agttagtaaa atgaagatga taaaagatat
    21061 tcgtttttgg gtttgtgtta taggaattgt catattaggc tttttatcag ggttgcttag
    21121 cggaaatccc ggaaaatatt actattcttt gcaacttcca ccatttgcac cacctagctg
    21181 gatatttggg ccgatgtgga ccttactgta tatattgatg ggtatttctc tttatttgtt
    21241 acttaatcac aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg tgattcagtt
    21301 catttttaat tttatatggt ctgctctatt ttttaattta agaaatattt tcattgcagc
    21361 aatcgatatt acacttttag ttattttctt gtcagtgctt atgtatcagt tgtggctgca
    21421 tcatcgcctg gcaatgtggc tcatgattcc ttattattta tgggtactat tcgcaactct
    21481 attgaattat tcaatttatt ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa
    21541 cacatagaaa aagtgccacc taagttagat tttttgtcta atttaggtgg cactctttat
    21601 taaccaaact atttttttct agtacccact tgttgttcca tactcattat tctggtcata
    21661 accataacca ttttgttcct gtccagcgta aggatcataa ctttgagtat cttgacttgg
    21721 tgtatagtac tgacttgtat cttctgtttg ctgataatag gtattcggat cttggtactg
    21781 atcatatgtg ttttctgaat aggaagaata atcggtgtac ccttcactat tcgatccatc
    21841 accgtttgtc gaatcgccat cttccgaatc atcaaagaac atcattgaag cattctcatt
    21901 tttcaaatta ggtaatgtat cgcttgtttt tatatttaat tgtttcttca gttcattttg
    21961 gatcgaaaga agatcatttt ttcctaaaat ttgatagtag atatcattga tcatttgtcc
    22021 ttcgccttgc aactggtctt gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac
    22081 atcaagcatg tcatcccaat ctaagtccgt cgtcacattg ttctcaactg cttttaaaat
    22141 ttttctataa ttgcttacgc catctaaact taaaacttta ttgacgattt tcgtcactac
    22201 ttcccgctga cgacgttgtc gaccgatatc gccttctgga tcttcgtgtc gcattcgaga
    22261 ataggctaaa cctttttctc cattcaatgt ttgttcacct actggtacat ggacaccatc
    22321 caaagtaaag tcaattttat tattcacttt gatgccaccg accgcattga tcaaatcttc
    22381 catgccatcc aaattgatcg ttacataatg atcaatagga atatcgagca gttgctcaat
    22441 tgaatccatg gccatttcga caccaccaaa tgcgtaggca tgatttaatt tatctaccgt
    22501 tccataacct acgatattcg tataaatatc tcggtccaaa ctgatgatcg ttgatttttt
    22561 ttgtttcgga ttaactgtta ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc
    22621 taatccaccg gtatctaaac ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct
    22681 tactctctca gaacgtttgg aggatattcg attagagttt ttagaaattt tgtttactgt
    22741 ttggctcgct tctcccataa atctgattcc gtatgctgat aatccggcaa tcagcaaaac
    22801 gaatacccct aacaaggtta atagaacttt ttgccatcgc ttcataatac acctctgttt
    22861 cacattttct aaaatattgt ctcatataac tgttcaatca gcaaattcac tttttaatca
    22921 taagaaaact ttaaaaaaca tttttgtgta acgattatta ttttttaaaa tttctcaaaa
    22981 ttcttaacta tcataccata ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca
    23041 atcgttttat ctttttttat gtattttttc cagaaaaagc gaacaacaag gtattttcca
    23101 aataatcgtg cagtaaaatg atcgcacata cctttcccat ctttttaaca gtctttgcct
    23161 accgttcaga ttatcattcg atttcacgac aatttcttcc tatgactggt ccttttgtta
    23221 aacccatctt ctattcgttt tatttccagc tcactgcatg gctcttaaaa tgactcgaac
    23281 tagtgattgc attaccatca tcttttaatg gatagatatg ctcctcttca aagtaaaacg
    23341 attttctttt aaagtaatag aattgacatg gtaatgagta tgtatactag tggtttcaca
    23401 aatggtcttt tattcaacat ccaaaaactg aaatttcttt aaaatccatt actaacgaag
    23461 aaaatgttcc ttgcttgata attagttagg acatgtcaag tgaacaaatg catttactta
    23521 gccaaaaaat ctaaaaactt cataaataga caaaaagaca cgactctcta agaagtcgtg
    23581 tctttattca acaaattatt ataatttagt cacttcagca gcttgaggtc cgcgtgcacc
    23641 ttcaacaatc gtgaattcta cttcttgacc ttcttctagg ctcttgaagc cgtctccttg
    23701 aattgctgag aaatgaacaa atacgtcatc tccgccgtct actgtaataa acccaaatcc
    23761 tttttcattg ttgaaccatt taaccgttcc gtgttccata aatgtatatc ctccaaaaaa
    23821 gttcttgcaa gtaaccttgc aagaacatta tacccaattt agaaaacgct agcaagcttt
    23881 cgctctttta ttttccaaat tatttttaag actgatatga cggaggaaat ggttattttg
    23941 ctgcttcgaa acgtttgttt acttcatccc aattaataac attccagaat gctgcaatat
    24001 aatctggtcg gacattttta tatttcaaat aataagcatg ttcccaaacg tccaaaccta
    24061 gaattggtgt ctttccttcc atcaatggag aatcttgatt tgcagtagag gtaatagcta
    24121 attttccatt ttccattaca agccaagccc atccagaacc aaatcgtcca gcggctgctt
    24181 ttttgaattc ttctttaaaa gaagaaaaat caccaaaagc ttcattaatc gcttctttta
    24241 tttctcctgt aggttcgcca ccagcatttg gtgccatgat ttcccagaaa aatgaatggt
    24301 tagcatgtcc gccaccattg ttacgtacag ctgtcttgat atctgttgga atagcgtcca
    24361 tatcagataa taattcttct attgtttttt cgcctaattc tgggtatttc tcaattgctg
    24421 aatttaaatt cgtcacataa gtattgtgat gcttatcatg atgcagatgc atcgtttctt
    24481 cgtcaatata aggttccaat gcgtcatatg cataaggtag atctggtaat gtgtaagtca
    24541 taaaaaaatt cctcctcaat gttttcttaa taatgtctac attacaaaga gtaacaaaga
    24601 gaaggaaatt cttcaattgt tatgcctagt cgtctttttc actttgacct acttcttctt
    24661 caaaaacctc agtttcttta aaaataaaca acttgctgaa aatatagttc gcaaggataa
    24721 caataatttg agtgatgatt ttcgccacat actcattgct gttcataaca ttgatcaaca
    24781 ataccattgc ccccatgtcc aatccgtaag aaaggatacg gtagaagaag aacttaccgc
    24841 attcaataag tgcatgtgta aaattcggtg attttgaatg gaatacccaa agtttgttcg
    24901 ttacaaaagc aaacaataca gaacaaaacc acgaaataga attggataag gccatactta
    24961 aatcaaaata ttggcgtgcg agagcaaaac taacaaaatt tacaatcgta gccaaaccac
    25021 caaaaaagag atagactagt atctcccaat atccttttgc ttctaaatat ccttttaact
    25081 gtcgatactg cttcatgagc caacctacct tattaaaaat tcttatttat catactctat
    25141 atttcaataa ctttcatctt tatttaaaaa aattaatttt ttttgatcct ttgcctttgt
    25201 ctctgcttgg atcgtcacat gtgtaatacc atattttaat tttaataaag aaattatacg
    25261 aggatatatc ttttcaatct cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa
    25321 aataattatc ttttcgctat actgccaaat atggacatga tgaacatcct ttaccccatc
    25381 gattgcttca atttcttgtt ccattttttc atagtcgata gcaggtgcac tttgtatcaa
    25441 aatagcggca gcttttttga tcaccattat cgcttctatc aatatatata tcgaaatgat
    25501 cagtgtaata actggatcga tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat
    25561 aatcacactt accgaagaca gtgtatcact taacaaatgc aaataactag attttatatt
    25621 caaactttca tctgcttctt gtcgtaataa taatactgag aacaaattcg cgagcaatcc
    25681 aataactgct actgttagca tcaaatgact atttatcttt tctggtgaag tgaaccgctt
    25741 gaatgcttca acggttaaaa acaaggataa tatgatcaat atgaatgagt tgatgaaagc
    25801 actcaatatt tctaaacgct ggtagccata cgtacgtttt ttatttgctt cttttcgggc
    25861 cattttttgt gccccgtaag aaaaaataat tgccaatgta tcactaaggt tatggaaagc
    25921 atctgaaaca agagacaaac tccctgacat aatccctcca ataaattctg ataaagtgat
    25981 caccgcattc aaaaccacta ctccaaatag tttttttgat gatctctgtg gctgtgtagg
    26041 cactattcct tcctccattt tatttatatt ggatagtctt tagacaagaa taacattttt
    26101 ttgatatttc accaatcatt cagctgtttc cttaaagcac ttgtatttca cttgatactt
    26161 cgctagaatt gcaagtgatg acactactag atgaggagta cttatgacaa caaaacaatt
    26221 aattatcagt tcctttatac gattcaaaga cctaaaggag gcgagaaatg tcccattttg
    26281 gaaagccatt gtctatcttt tagttttgag tataattatg gctttgccaa tctcttttca
    26341 agtttttcaa gtcttaaaca acattcaatt ggatgggcaa aagattgcta ctcgcattcc
    26401 tgattttaca atccaaaacg ggcagcttca aaccgaagaa aaagaaggat tcatttacca
    26461 aacaaactcc attatcttca catttgatcc agagggaaaa cgaacagaac aggacatctc
    26521 tactgatttg atggggaatt ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc
    26581 tcttcctaat acaggcacga cttctgcttt gttgaacaat aaccaattag aacttcccta
    26641 caccaatgaa tctttaaaaa acttaacagg taagcagctc cgttcttttt taagtgaagc
    26701 atctatacca ctttggataa aagtcctgac atttatattt tctatttatc ctagttttct
    26761 gaatctgctg atcactttgc tatttaccaa cattgctgct tttctctatg cacgctttcg
    26821 actaacaaaa gctacttttc ttgattgttt aaaaacattg atctattcaa tttcattgcc
    26881 tactataatt gcaacaatct tgatggtctt tcttccttct tttgacacta gcgccttcat
    26941 tgcttttgca ggaatcttta tttttgctca agctgtcaaa ggatgggcaa aaatatcgat
    27001 caattaagaa ataaagacaa ctcgttattt ctacttcaat agaccctttt agaggttgtg
    27061 acattttttt gtcacagccc cttttttatt tgaaaaatat ttctttctca aaaaacaagc
    27121 aaggcgcttc aaagttcttc ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt
    27181 aactatgtca gaaagaatca tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga
    27241 aatccatact atagcgcctg gttatatctt taaaacagaa ttacaaccag aggatctaca
    27301 aaatgtagag atcagtttgg gctggaactc agactaccaa gaaaaactat tagcctcttc
    27361 taccttgaaa tgggtccaat ctatctcagc tggtgttgat cagctacccc ttgagacttt
    27421 ttcaaagaaa catattttac tttcaaatgg tagtggcatc cacagtgaat cgattacgga
    27481 gcatgtctta ggaatcatcc ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca
    27541 gagtgacatg aaatggttag gttcatcggt ccattaccaa tctatccaag ggaaaaaaat
    27601 actaatcatt ggaactggcc atattggaaa aatgattgca aaaaaatgca aagcattaga
    27661 catgaactgc tatggaatca atacatccgg tcatccagca gaaggaatgg aaaagacttt
    27721 ttctttaaat caactaaagg aagttcttcc tgaagtagat ttcgtagtca atatcttacc
    27781 tctaacggag gaaacaactg ggctctttga tcagcatatg tttgaacact ttgatccaaa
    27841 atctgtattc atcaatgttg gccgtggtgc ttctgttaag acacaagatc tggtgcaggc
    27901 tttaaataac cagcaacttt cctttgcagc attagacgtt tttgaggaag agcctcttcc
    27961 acaagatcat cctctttgga aaatggacaa tgtactgatt acttcccaca ttgcaggttt
    28021 gacacctgat tttcaaaaga aattaatggc tattttttta accaatctga aaagctactt
    28081 tgccacccat gagcttcaaa ccaatcaagt aaaattgtct gcaggatatt aatagctgaa
    28141 aacaatattt tcaaaagaaa aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt
    28201 tattgttttt taaagtgata ggttggctcc tccaggttgg gattataatc aacaagtaaa
    28261 tcatatcctt taaagaacca ttcatctgct tcctcgataa aaaaaagtgt cccatcaatt
    28321 tccactttct ttacaggatg ttctggctgt tcaactgaca ttccaatcga aaaaccatca
    28381 tgaacctctg ttttgccgta gacttttcca aaaaaacgta ttccgtaagt ttctggcaga
    28441 tcaatttctg agtcaaacca cttttgtgct tttggcgtta ccgttaattt catcccaaca
    28501 acctactttc tcttttcttt ctattgattc atcttatcgt ctagaatatc cacatcgcct
    28561 gtctctccaa taaccagtag attatcattt tcacgaacat attcatctgc tgcaggagaa
    28621 gcgataaccg tttgatctac tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg
    28681 aaatttaatt cagctaatgt tttgttgaaa aatttaggat tggagactcg gatttctgct
    28741 aaagaatatt tatctgacaa ctctaggtaa tctaaaatat tctttgacac taaattatgc
    28801 gcaatcctta ttcccatgtc tcgctcggga tggacgacgc gatcagctcc gatcttttca
    28861 agtactctgg catgatactc attttgggct tttgctaaaa tatttggtac gcccatttcc
    28921 tttaccatca gagtaactaa gatactggct tgaatatctt ctccaatagc gacaattaca
    28981 tgatcgaaat ttcttatgcc taatgatcga agtgtcattt catcctgtgc atttgccaca
    29041 acagcatgag tggctatatt catatattca ttgacccgat cttcattact atcaatagcc
    29101 agtacttctt gaccagcttc gatcagtgtt cggcagatac ttccgccaaa tcgtccaaga
    29161 cctacaattg caaaattttg tttcaccgtt ttcctccttt agtatcttct tttctcagta
    29221 taacaaagcc tttacaaaaa caaaagcaag catcattatt gtaaaaaagc caagaaaaat
    29281 aatagttatt ttctcagctt ttgattatcc tctttatcat cgtttttatt tcaccaaacc
    29341 atgtttgaat gcatagatag cggcttgtgt acggtcttcc acttctagct tagccaatat
    29401 attggaaaca tgtgttttta ctgttttcag tgtaataaac aattcgtccg ctatttcctg
    29461 attacttttt ccttcagaaa tcagcattaa aatttcattt tcccgattcg ttagttcttc
    29521 atgtaataca ggctcatttc gccgactcat tttttccatc atttttgttg tcacttctgg
    29581 ttctaatact cgctcgccac gttgcgttgc ccttatagca tcagcaattt catgtgcggt
    29641 agaggtcttc aacaaatacc ctgctgcacc tgcttcaatg gcaggataaa ctttttcgtc
    29701 atcaataaaa ctggttacga taatgatttt agcttctggc caatctttta agattgcttt
    29761 tgttgattcg ataccatcca tttcttccat gactaagtcc attaaaatga cgtccggtcg
    29821 aagtgtcatc gccttctcat agccttgtcg tccattttca gcttctccaa ttacttcaat
    29881 gtcttcttga atagataaat aaaaagagac gcctaagcgc accatttcat ggtcatctac
    29941 taataaaact tttatcatcc ttatctatgc ctccttcatc aaagggacct tgatttctac
    30001 acttgtacct tgacctttaa aactaatgat ttttactgtt ccaccaatac cagctactcg
    30061 ttctttgata ttgttcaatc cataacttcc cgttttcgtt tcattcatat taaaccctgt
    30121 tccgtcgtca ataatacgta acaaaagatt attgtctatt ttgtgcaaat atacctctaa
    30181 ttcattggct ttagcatgtc ttaatgtatt tgataacaat tcttgaacga ttcggaacaa
    30241 gtgatcctca atggaactag atagttttac atcttctaca tcccatttca gcgaaatctg
    30301 gattttattt tgcaattcct tcaataactg ctcgatacct tgctttaaac ttttctcttc
    30361 taaattgact ggacgtagat gaagaagcag cgcacgcatc tcagactggg aagcgttgat
    30421 gatctctgct accattttca actgcttgcg aaacatttct ggtgtctcgc ttttttctgc
    30481 ttgctccgtc aatgccgaca tcatcatcat tgctgcaaac aattgttgcg aaactgaatc
    30541 atgcagctca cgggctaatc gatgtctttc taattctaaa atttcttctt tggtttgtcc
    30601 atctacatat tggggacggc tcgttacaat ttgtaattca ctagatactt caatcatttt
    30661 ttcttttatc ttagtgattt ccttatcaat atctttgatg tataaatcgt ccgatgcact
    30721 tgggatcgga agaagcaaca atttggattc ataattgcca gaagaaagtg cacgcagctt
    30781 ttcttcgatt ttcccgtatt ttgtcttttg aataagtgat aataatagaa agacaattaa
    30841 tcctacacct aaggaaatgg ctaacaaatg gaagataagc ggaacatata ataaacgtgc
    30901 acgcagtaat tctccccacc aatgactttg attgctggca taaaaataag tgaaaagtga
    30961 aaatagaata ataaggaaag ccgcaatccc cgaataaaca gctagcatcg ctctggatat
    31021 ttttcccatc atattcgtat cacctcgaca tctccaatca aggtattagt aataatcttc
    31081 aatctacgag ggttttcatc ataatcactg ctgtagattt tgatttgttc atttttcaaa
    31141 gaaaattgct cttcttcaaa caagacattt cctactaatg tcgcatgctc taatcggatg
    31201 gctacgccca aaggtactaa gatccgtgtt ctgcctatcc cttttcttac aatcacaata
    31261 ttatcatctt ttggcaaaag cgtattgccc aaatctatga tagtatcacc agaaatgacg
    31321 gcgatgttga tatcgtccca ttcatagacg tcgtttccaa tacgttggtt accgaataat
    31381 tgctgtcttt tccgctcatt attatgtgtt tttaattgct ctgtttggat catcatgatc
    31441 tgtttctttc gccaaaaagc atttttggtt aaatctatcc ctgaaatctc tacacctttc
    31501 agaccaataa ataaaacggc aaatacagcc atcatccata aagcaggact gttgactaag
    31561 ctaaaaaaaa tgaccaaact tcctaatatc aattgaaaat tttgaaattt agtacgagga
    31621 tgtttgcgca tagctagata tatattgaaa ataccgaata tcactaaaag caaaagtcca
    31681 gtattgttta cgatttgcca aacagcaaat aacagcagta gtgcttcaac taccacaaaa
    31741 aaacgccaag aactattcat cgctctttct ccctcctgac acattctcat ttcatcatac
    31801 ctattttttt aactgttggc tataggactt tagatgtaag atcgtttgtt gaaatctaaa
    31861 atacacaaag agaaaaagat ctagagtgta tcatcagtaa gatgaactct ctagatcttt
    31921 cagtcagact acgcttgttc tactttgacg attttaacta acatatcgcc acctggagta
    31981 gcaatagtta cttcatcatt tttacgtttg ccaatcaatg cttgcgcaat aggagaatca
    32041 ttggaaattt tgccagaaaa cggatctgct tcagcgcttc cgacaatcgt gtactcttct
    32101 tcttctccat ctggtagttc gatgaatgta actgttttac caattgatac ctcatctgca
    32161 tctacaccgt cgttatcgat gatttgagca aaacgaatca tattttcaat tgttgtgatt
    32221 cttccttcaa caaatgcttg ttcatctttc gctgattcat attcagagtt ttcggaaaga
    32281 tctccgaaac cgcgggcaat tttgattcgt tcaatgattt ctccgcgttt gactgttttt
    32341 agttcttcta attctttttc taatttttct ttaccttcta gagtcattgg ataaactttc
    32401 tctaccatgt gtaacgtctc cttttttcga taaaataccg caaaaggtaa tcttttgagg
    32461 aatatagtcc aaaaaagatt accacgcttt tcttcaaatg taaatgtttt tctaaaaatg
    32521 atcaatgtct agagcctgtt tttctaagaa ttatttacat atttttccac taattccata
    32581 tgttcttcat aagttttagc gaaataaaca tttccagtgc tcgtatctgc tacgaaataa
    32641 taatagtcat tttctgctgg ttctaatact gccttgattg cctcttcgct tggattgtca
    32701 aaaggtccag gtccatagcc ggtattggta tacaaattat atggtgaatc tacagctgta
    32761 tcttcatacg tcactaattc tttgtgttca cctaatgcgt ataaaacaga gatatctgac
    32821 tgaagtggca tatttgcttt gattcggtta aagaagactt gtgcaatgtt tttacgatca
    32881 ttttctttta cgccttcttt ttcaactaga gaagctagag ttaaaacttc ttgtacagtc
    32941 aaatttttct gcttgatagc tgaataatag ttttgcatga ccgtattcgt cttgttcacc
    33001 atttcaatca ctagatcctt aagcgaagta ctcgtgtaat aatcatatgt ggctgggaat
    33061 aagtaacctt ccaaacggta ttttgtgttc tcagctttag atgcactttc caacagttct
    33121 gggaattttt ggtgcaactc gttaaagaaa gtctcatcat tcactaaatc taaaaattct
    33181 ttcttgtctt taccagttac cttcgctacc cgttcagcaa tctgatcgat atcgtaccct
    33241 tctggaatgg caattttccc atctgcaact tttgttggct cagaagtacc gccttcttgc
    33301 aactgtttgc caatctcgtc aagagtcata ttaggcgcta actgataata gccagcttga
    33361 aagccagtta aatttttaaa ttttgtataa taattgaaaa caatcccgct tttaataata
    33421 ttgtccttct ccaaaatctc accaatctgc ttgttagaag agccactcgg gatctcgact
    33481 tggactaatt ggtcatctga tttatctaaa ggcttcaacc cgctatctac ataacgatac
    33541 acggtaaatc caagaaatcc gccaataatc aataatgtaa gagcaatcac taaaatgatc
    33601 ttcctcacaa tcttatcttc ttttttacgc agatttctcc gcttgtcgat tatatctgct
    33661 gtagctgcct tactggcttc ttttttatct ttacttgggt gttgtttgtt tcctgacaaa
    33721 acaactcctc cttactatca agttcgtaac tttattcatt atacatgata tttgaattgg
    33781 cgtgaatgat tttatttaaa tattctatca aaaatgtaag tagtaaatcc attactataa
    33841 gattaaaata aatgttatca agatacaagc tatttt
//
LOCUS       DAYYME010000016        32545 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000016 DAYYME010000000
VERSION     DAYYME010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.016"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(166..354)
                     /gene="rpmB"
                     /locus_tag="ACHBFW_000623"
     CDS             complement(166..354)
                     /gene="rpmB"
                     /locus_tag="ACHBFW_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFM9316964.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            624..986
                     /locus_tag="ACHBFW_000624"
     CDS             624..986
                     /locus_tag="ACHBFW_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9316965.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            1038..2720
                     /locus_tag="ACHBFW_000625"
     CDS             1038..2720
                     /locus_tag="ACHBFW_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFM9316966.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2837..4873
                     /gene="recG"
                     /locus_tag="ACHBFW_000626"
     CDS             2837..4873
                     /gene="recG"
                     /locus_tag="ACHBFW_000626"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFM9316967.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEGSEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4922..5923
                     /gene="plsX"
                     /locus_tag="ACHBFW_000627"
     CDS             4922..5923
                     /gene="plsX"
                     /locus_tag="ACHBFW_000627"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFM9316968.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            6045..6287
                     /gene="acpP"
                     /locus_tag="ACHBFW_000628"
     CDS             6045..6287
                     /gene="acpP"
                     /locus_tag="ACHBFW_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM9316969.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6555..7559
                     /locus_tag="ACHBFW_000629"
     CDS             6555..7559
                     /locus_tag="ACHBFW_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9316970.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7560..8504
                     /locus_tag="ACHBFW_000630"
     CDS             7560..8504
                     /locus_tag="ACHBFW_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9316971.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8504..9466
                     /gene="opp4B"
                     /locus_tag="ACHBFW_000631"
     CDS             8504..9466
                     /gene="opp4B"
                     /locus_tag="ACHBFW_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM9316972.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9493..10407
                     /locus_tag="ACHBFW_000632"
     CDS             9493..10407
                     /locus_tag="ACHBFW_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9316973.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10433..12214
                     /locus_tag="ACHBFW_000633"
     CDS             10433..12214
                     /locus_tag="ACHBFW_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9316974.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12562..13248
                     /gene="rnc"
                     /locus_tag="ACHBFW_000634"
     CDS             12562..13248
                     /gene="rnc"
                     /locus_tag="ACHBFW_000634"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFM9316975.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13268..16849
                     /gene="smc"
                     /locus_tag="ACHBFW_000635"
     CDS             13268..16849
                     /gene="smc"
                     /locus_tag="ACHBFW_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFM9316976.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16858..17667
                     /locus_tag="ACHBFW_000636"
     CDS             16858..17667
                     /locus_tag="ACHBFW_000636"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9316977.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17678..18676
                     /gene="ftsY"
                     /locus_tag="ACHBFW_000637"
     CDS             17678..18676
                     /gene="ftsY"
                     /locus_tag="ACHBFW_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFM9316978.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18863..21130
                     /gene="gshAB"
                     /locus_tag="ACHBFW_000638"
     CDS             18863..21130
                     /gene="gshAB"
                     /locus_tag="ACHBFW_000638"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFM9316979.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21219..21671)
                     /locus_tag="ACHBFW_000639"
     CDS             complement(21219..21671)
                     /locus_tag="ACHBFW_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFM9316980.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            21821..22771
                     /locus_tag="ACHBFW_000640"
     CDS             21821..22771
                     /locus_tag="ACHBFW_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM9316981.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22764..23723
                     /locus_tag="ACHBFW_000641"
     CDS             22764..23723
                     /locus_tag="ACHBFW_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM9316982.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23720..24475
                     /locus_tag="ACHBFW_000642"
     CDS             23720..24475
                     /locus_tag="ACHBFW_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9316983.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24498..25451
                     /locus_tag="ACHBFW_000643"
     CDS             24498..25451
                     /locus_tag="ACHBFW_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9316984.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(26095..26391)
                     /locus_tag="ACHBFW_000644"
     CDS             complement(26095..26391)
                     /locus_tag="ACHBFW_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFM9316985.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26746..27102
                     /locus_tag="ACHBFW_000645"
     CDS             26746..27102
                     /locus_tag="ACHBFW_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM9316986.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            27112..28011
                     /locus_tag="ACHBFW_000646"
     CDS             27112..28011
                     /locus_tag="ACHBFW_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9316987.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            28531..29259
                     /locus_tag="ACHBFW_000647"
     CDS             28531..29259
                     /locus_tag="ACHBFW_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM9316988.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKSVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            29404..31431
                     /locus_tag="ACHBFW_000648"
     CDS             29404..31431
                     /locus_tag="ACHBFW_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFM9316989.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            31539..>32545
                     /locus_tag="ACHBFW_000649"
     CDS             31539..>32545
                     /locus_tag="ACHBFW_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM9316990.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     W"
BASE COUNT        10851 a         5690 c         7004 g         9000 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaataaaaaa ccgttcacta tctgaacggt ccaagtatca ttcaattaaa cgcgttcaac
      181 ttttcctgat ttcaaagcac gagttgacac ccaaactttt ttaggttttc cgtcgaccat
      241 aacgcgaact ttttgtaagt taggtttaac agtacgtttt gttgagttca tagcgtgtga
      301 acggttgttt ccactttttg tcttgcgacc tgtaaagtaa caaacttttg ccatggtaat
      361 ttcctccttt gccttgattt ggagcaatag aataattcag ctcataccag actaacatat
      421 catatagaat ggaaatttgc aagtacattt tcatagatac aattgacaat tgagaagaat
      481 aattgtaaat taaacaaggt tatacctgaa attttgcaag gtttttgagt actttgggta
      541 actgcctttt atttcaagtt gcgctatgat aaaatatgat agaagaaaaa tgggagtttt
      601 ttacccgtta aggaggctca ttcatggctg taaaaattca aacgtcagct ggaaccattg
      661 aaattaccaa tgatgtaatt gcaacagttg ttggtggcgc cgcgacagat gtgttcggtg
      721 ttgtaggaat ggccagcaaa aatcaaatta aagataatat tactgagatt ttgcgtaaag
      781 aaaattactc aagaggagta gttgtccgcc aagaagataa tggtattgct gtagatgttt
      841 atacgattat cagctatggt acgaaaatct ctgaagtttc ccgcaacgtc caagaaaaag
      901 tgaaatacaa tctagaggca ttattaggcg taactgccaa ttcagttaat gtctttgtac
      961 aaggtgttcg tgtactgcca gactaggcag gagactggct ggtaactcag cagtcgatct
     1021 gaaggaggat tatctaggtg agtgtaacag aaataagtgc aagccagttc caggaaatgg
     1081 tccaggcagg tgcgaatcgt ctgcaagtaa atgcagaata cgtcaattca ttaaatgtat
     1141 tccctgtgcc agatggcgat acgggaacaa atatgaatct atctatgact agtggagcta
     1201 aggcagtagt agattcaacc tcagaaaaag taggagaatt agcagcaatg ctttcaaaag
     1261 gattgctgat gggtgcgcgc ggaaattccg gcgttatttt gtctcaattg tttcgtggat
     1321 tttctaagca aattccagat gttgtctcat taaatgcttc cgatcttgct gcagccttca
     1381 ctcatggagt tgaaactgcg tataaagcag taatgaaacc agtagaagga acgattttga
     1441 cagtagctag agaagcagcg aaagctggag aaaagaaagc aaaaagtact gatgatgtcg
     1501 ttgaagtaat gacagctgtt gtaaaagggg caaagcgtgc tttagctaaa actccagact
     1561 tgcttccagt cctaaaagaa gtcggtgtcg tagacagtgg agggcaagga ctgctgtttg
     1621 tttatgaggg ctttttaagt gcattgaatg gagaattcca agcggatgaa acatatgaac
     1681 cttctccagc agaaatggat gatatggtca atgcagaaca tcatcgcagt gtccaaggtc
     1741 aaatggcgac agaagatatc cattttggtt actgtacaga aatcatggta aaaatcggag
     1801 aaggacctac agtcgatagt acattcgatt acgaagaatt ccgtaactat ttagatggta
     1861 taggagattc tttacttgtg gtcaatgatg acgagatcat caaagtccac gtgcatacag
     1921 aaaacccagg agaagtcttg aattatggac aaaaattcgg ttcattagtc aaaatcaaag
     1981 tcgacaacat gcgcttgcag catgagacga ttttggaaca cgatgaagaa gtgaacgagt
     2041 ttgcgaaaaa agaaacacca cgtatcccgt atggtatcgt agcgatcgct gctggtaaag
     2101 gcgtccaaga gctgtttgaa agtttaggcg ctaattatgt tatcagtggt gggcaaacaa
     2161 tgaatccaag tacggaagac attgttaaag cgattcaaga agtaaacgca gataaagtga
     2221 ttgtcttgcc aaacaataag aacatcttca tggcagctga tcaagcagca gaagtagcag
     2281 atatcccagt agcagttgtt ccttcaagaa cgatttccca aggattgaca gctatgcttt
     2341 cattcaatga tcaagcttct ttagaagaaa acaaagaaac gatgacagaa atgttgtcta
     2401 gcgttgtgag cggtcaagtc actcacgcga tcagagatac ctcaattgac ggtgtagaga
     2461 tcaaagaagg agactttttg ggcatgatcg atggaaaaat cctgatctct aacccagata
     2521 tcacagcaac agcgttaagt acactagaac aaatgatcaa tgaggacaca gagatcgtaa
     2581 cgatcttggc aggtgatgaa ggaacagttg aacaagcaaa tgaatttgct gagaaactaa
     2641 cagaaaaaca tcctgatttg gaaatcgaga tccaccaagg agatcagcct gtctatcctt
     2701 atctattttc tgctgaataa tttctgtgtc agaatatctt tctaacacag tattcattca
     2761 taaaaggcct tcggttagtg atcaggtatg atcgctgacc aagtcctttt tttgttaagg
     2821 gagggaggaa aaatttatgc gttctttaga tgatccaatc actatgctgg caggagtcgg
     2881 agaaaaaaga gcagctagtc ttgccagctt gggcatccaa acgattgaag acttactgac
     2941 ttattatcct ttccgctacg aagatattca ggaaagaaat ctaaacgaga tccaagatca
     3001 agaaaaggtt actttgaaag gaatcgtagt ctcgcctcct gtgatgaatc gatttggtta
     3061 taaaaaaagc cgattgcaat ttcgtatgat gcaagaacat gatgtgttca acgtttcttt
     3121 ttttaatcaa ccttatctaa aagaaaaagt cattttatcg gaggaaatcg ccgtatacgg
     3181 taaatgggat gcaaagcgta aagcattgaa cgggatgaag attttaggtt ctcaaacaat
     3241 ggatgacttt tcgccgatat atcacgtcaa caaatcaatt cgtcaaacga cattagttga
     3301 tctaatcaaa aaagggtttc aagaatacgg cacattgatt gaagaaaatc tgccaaacaa
     3361 ccttgtggag aaatatcgac tgcttgaccg agcttctgct gtaagaagta tgcattttcc
     3421 aaaagaccac gaagaaaacc atcaagccaa acgacgtgtc gtttttgaag agttcttttt
     3481 atttcaaatg cgtcttcaag gattgaaaaa agcagaaaaa gcagaaacga atggtttgga
     3541 gatcctttat gatgtccaaa aattgaaaga atttactcaa aagctacctt ttgaactgac
     3601 aaatgcacaa aaacgtgtaa cgaatgaaat ctgtcgggat cttcgcagtc cgcagcatat
     3661 gcagcgactt ttgcagggag atgtaggtag cggaaaaaca gtggttgccg cgattgcgtt
     3721 atacgctacc gttacagcag gtttccaagg tgcgttgatg gtgcctacag aaatcttggc
     3781 acaacaacat atggaaagct tgcaacagct gtttgatccc aatgaagtaa ccatcgcatt
     3841 gcttacgggg tcaacgaaag caaaagaacg tcgtgagctc cttgaaaaac tagagcaagg
     3901 ggaaattaac atcgtgatcg gcacacatgc attgatccaa gatggagtcg aatttcagaa
     3961 tttaggtcta gttattacgg atgagcagca ccgttttggc gtgaaccagc ggaaagtact
     4021 tcgtgaaaaa ggctggcgac cagatgtctt atttatgaca gcgactccga ttcctagaac
     4081 attagctatt acagcatatg gtgagatgga tgtatctatc attgatgaac ttccagctgg
     4141 acgtatcccg atcgaaacac gctgggtaag aacacctcaa ttggacagtg tactggattg
     4201 gacgaaaaaa gaactggcaa gaggacatca aatgtatgtc atctgtccgc tgattgaagg
     4261 atcagaagca ttagatgtaa aaaatgcagt ggaaatctat gaaaaacttc gtgaactatt
     4321 tgcaccaaaa tatgaagttg gcttgttaca tggtaaaatg aagaatcagg aaaaagaagt
     4381 aatcatggaa gccttcaaag acaaccagat gcagatatta gtttcgacta ctgtaattga
     4441 agtaggggta aacgtgccga acgcgaccgt catgctgatc atggatgcgg atcgatttgg
     4501 attggcacag ctgcatcaac ttcgtggacg tgttggacgc ggaagtgatg cttcctactg
     4561 tatcttagtt gctaatccta aaaatgaact aggtgtggaa cggatgaaaa tcatgacaga
     4621 gaccaccaat ggttttgtac taagcgaaaa agatctggaa ttacgtggtc ctggtgaagt
     4681 atttggcttc cgtcaatctg gtctgcctca atttgcgatt gctgacttgg tgacggatgg
     4741 aaatgttttg gaggtagcca gagaagaggc tgcagccctt tggcagataa aggattggca
     4801 gatgcttcct gagtatcagc ctttagcgca aagcctccaa atagaaaatc aggataatcg
     4861 ttatttcgat tagcagactg tgctatactt tatactgatg acggaaaaag gagtggaaac
     4921 aatgaaaatt gccgtagatg caatgggcgg agatcacgca cctcaagcaa ttgtagaagg
     4981 cgtggcttta gcgcagcaag attttccaga agtagaattc ttactttacg gaaaagaagc
     5041 agaaatcaag aaatacttaa caaatgaaaa aaacatcacg attatccata cagacgaaaa
     5101 aatcaatagt gatgatgagc cagtaaaagc aattcgacga aaaaagactg catcaatggt
     5161 ccttgctgca caagcagtga aaaacggtga agcagatgct gtattttcag cagggaatac
     5221 aggcgcgcta ctagcagcag ggttgttcat cgtaggacgt atcaaaaata tcgaacgccc
     5281 tggcttgatg tctaccttgc cagtagtagg gaaagaaggt gccggtttcg atatgctgga
     5341 tcttggcgct aacgcagaaa acaaaccaga acatttgttg caatacggta ttttaggttc
     5401 tttttatgca cgtaaagtcc gaggtatcgg acgtcctcgt gtcgcactct taaacaacgg
     5461 aacagaagca acaaaaggta gtgaagtgac gaaaaaagcc tatgaactcc ttcagaatga
     5521 aacaagcttg aattttattg gaaacgtaga agctcgtgag ctactcaatg gtgtggctga
     5581 tgtagtggtc acagatggat ttacaggcaa cgctgttttg aaatctattg aaggaacagc
     5641 aatgaatatg atgaatctat taaaatctgc tattctaaat gaagggatca aaggaaaaat
     5701 gggcgctatg cttttaaaag acggcttacg ttctttgaaa gcagaaatgg attattccaa
     5761 acatggcggt gccgttttgt ttggcttgaa agcaccagtc attaaaactc atggagcaac
     5821 aggccccgat gctgttcgct atacgatccg ccaaatccac acaatgcttg agacagatgt
     5881 agttggacaa ttagtcgaac aatttgaaaa aagcgaagaa tagtatttgg atatctgaag
     5941 caatccgttc cattgaaaaa aaggtggaca ttcctgaaaa atgccagtaa aattagccat
     6001 ggtgtaggta attgacaagt gtatgattct tggaggtgaa aaagttgact cgcgaagaag
     6061 tatttaacaa agtagcgaaa attatttcaa atcattttga gatagatacc gataaagtga
     6121 cggatgaatt gaacatcaaa gatgatttaa aagctgattc aattagtatt atggaattcg
     6181 ttttagagct tgaagatgaa ttcggtacgg aaatttcaga tgaagacgct gaacaaattg
     6241 aaacagtcgg tggcgcagta gattatatca gcagccatct taaataattt caaataaaag
     6301 caaagaagga taaaaagctg catacagctt tttatccttc tttttttaat ctttttgaca
     6361 cgtaaaagtc atttttctga taattctggc gataaacaga aattatgttg caaagtaaag
     6421 cgattttcta tataattatt aataattcat tacacaaaga taagaataac ctttgttata
     6481 aaacgcttta ttctgtgtaa tgtccttttt tattaagaaa aacttccatt taggaagcag
     6541 agaggggtat tcgattgtcc gataaccaat tattagaagt tcagaaccta catacaggat
     6601 tccgactcaa agatgactat tacgatgcag tagatgacgt ttcatttact ttagacaaaa
     6661 acgagatttt agcaatcgtt ggggaatctg ggtgcgggaa aagtaccttg gctactacga
     6721 ttatgggatt acttgatcct aataacacaa aaattacagg tgagatttta tataacgatc
     6781 tgaatttaat cgacctgaac gaaacgctct ataataaaat ccgcggaaat gatatcggaa
     6841 tgattttcca agatccgttg tctgcattaa atcctctcat gagaatcgaa gaccagatca
     6901 aagaaggact ttcctatcac acaaagatga atgcagaaca gcgtcaagct cgtgcactag
     6961 aattgctaga acaagtaggg attcctaatc ctgcgagagt tggacgccaa tatccccatg
     7021 aactatcagg cggtatgaga caacgtgtca tcatcgcaat cgcaatcgcc tgcaaacctc
     7081 caatcgtcat tgcagatgaa ccgacgacag ccctagatgt aaccatccaa gcacagattc
     7141 ttgatttact gaaagacctt caggaagaaa caaaaacagg gatcatcttg atcactcatg
     7201 acttaggagt cgtagcagaa atggctgatc gagtggcagt gatgtatggc ggacagttcg
     7261 ttgaagtagc aggtgtaaaa gaactgtttg atcatcctaa gcatccttat acacgctctt
     7321 tactgaactc tataccacaa gaaggaaatc atgatgcaga actccatgtg atcgaaggtg
     7381 tcgttccttc tctgaaaaac atgcctcgta aaggttgtcg gtttgcacca cgtattccat
     7441 ggataccagc aagcgatcat gaagaagagc ctatccttca tgaagtcgca ccgaaccact
     7501 tggtacgttg ctcctgctat aagcatttcc actttagaga cgaaaaagga gaggtgtaga
     7561 tgacagaatt aatcacaatc aaagacctga aagttcatta ccctattcgc agtggattct
     7621 tcaatcgagt aacggatcat gtatatgctg tagatggtgt ggattttatt atcgaaaaag
     7681 gaaagacata tggtttagtg ggagagtctg gctctggaaa atctactacg ggaaaagccg
     7741 ttgtgggatt agaaaaggta acagccggac aaatcatgta tgaaggaaaa gatgtaacga
     7801 agcgaagcaa ccgcaaaaaa atgggttata acaaggatgt gcagatgatc ttccaagatt
     7861 caatgtccag tttgaatcct aaaaagcgag tgttggatat cattgctgaa cccatccgaa
     7921 attttgaacg gttaagcgat caagaagaaa agaaaaaggt aaaaggactg ctagatatcg
     7981 ttgggatgcc ggaagatgcg ctatataaat atcctcatga attttcaggc ggacaacgtc
     8041 aacgtctagg agtagcccgt gctgttgcta ccaatccaaa actgatcgta gccgatgaac
     8101 ctgtatctgc tctggatcta tctgttcaag cgcaagtatt gaatttcatg aaacgaatcc
     8161 agcaagaata cggcctcagc tatttattca tttcacatga tttaggtgta gtaaaacaca
     8221 tgtgcgacaa tatcgcaatt atgtacaaag gtcgatttgt agaaatcggt acaagagaag
     8281 acatttataa tgacccgcga catatctata cgaaacgtct gctttcagct attccgcaaa
     8341 tcgatgtaga aaatagggaa agccacaaag aaaatcgacg aagagtagaa caagagtata
     8401 tccaaaacca aaaagaatac tatgatgcga ctggtcgagt ctacgatttg cgtacgatca
     8461 caactacaca caaagtggcg ttgaaagatg gaggtgcaag ctaatgtgga aaacgattct
     8521 gcgacgtgtt ttattgatga tcccgcaagt tattatttta agtgttctga tcttcttatt
     8581 agcccaagcg atgccggggg atccatttac cggtttgatc aatccaaatc aggatccggc
     8641 agtgatcgaa cagatgcgac aagcagctgg cttgaatgac ccatggtatg tccagtactt
     8701 ccgttggatc ggaaatgctt tgcaaggcga ttttggtcaa agtttcttat acaaacgctc
     8761 tgttgctgac ttgatcggcg aacggatcat caacacttta tatctatcga tcttgacagt
     8821 tgtccttact tatttgattg ctgtgcctct ggggatgctt gctggccggt atcaaaattc
     8881 aatcttggat aagattgtcg taatctacaa cttctttagt tttgctgttc ctttatttat
     8941 cttcggcttg atcatgctat ttgtttttgg ttatcgttta ggctggttcc caacaagtgg
     9001 atcacaaaca ctaggtttgc aaggtggatt tatcactcaa tggatagacc gtttgaaata
     9061 tattctgtta ccatcaatta cccaagcatt tttggcgact gccgtcacaa tccaatatct
     9121 gcgaagtgaa gtaatcgatt cgaaatcgct cgattttgtc agaacagccc gttccaaagg
     9181 ggtaccgacg aataaagtat tcagtcgaca tattttccga aatgcagcgt tgccgatggc
     9241 ttctcaaatg gggtatgaaa tcacttcgtt gattgctggt tctgtagtta tcgaaaagat
     9301 ttttggttat ccgggagtcg gaaaactatt tattgattcc atcggccaac gggactatac
     9361 cgtgatcaca tccttagtat tgattttagg aatcgcgacg ctagtgggga cattactttc
     9421 agatatcatc atgagtatcg ttgacccgcg tattcggatt caataaaacc aatcagaaag
     9481 gagaatggat cgatgagtga aaaaaacaat acagcagttc aagaaagtat tcctcctatg
     9541 gggatacgaa tgattgctcg agaatttaaa aaagataaaa ttgctatttt ttcattaggt
     9601 ttgctagtgg ttttattatt agtgatcttt atcggtgcga tgctgaccga tcaagacaaa
     9661 gtgatgtatg ttagtatctt cgacaaatac gctgcaccag gaacagtttc gacacaagga
     9721 caaaagttca ttctgggagc agatgaaggc ggacgtgatg tattaggaca attgatcata
     9781 ggtgctagaa actctgttac gatcggattt gcgatcacga tccttacttc gatcatcggg
     9841 gttggattag ggatcatctc tggctactac ggagggatca ttgacaacat cttgatgcgg
     9901 atcgttgatt ttatcatgat tctaccgatc atgctgatca ttatcgtctt cgtatcgatt
     9961 atttccagat acaatgtctg gtcctttata ttcatcatga gtgcatttta ttgggtagct
    10021 aaagcccgat tattccgaag caagacatta tctgaagcaa gaagagatta tgtcagtgcc
    10081 tcaaaaacaa tgggaaccaa tgattttaaa atcatgttcc gcgaaatcat gccgaatcta
    10141 agttcattaa tcattactaa tttgacgatg aactttgcgg caaacatcgg tatcgagaca
    10201 actttgactt tcttaggttt cggcttgccg gcaaatgtac ctagtttagg tacattgatc
    10261 ggttatgcaa gtaacggaga agtcttagtg aatcggcaat ggatctggtt acccgcatca
    10321 atccttattt tagttttgat gttgagtatc aattatgttg gacaagcatt caagcgttct
    10381 gcagatgcgc gccaacggtt aggctagtat agaagcgaag ggggaaatag gaatgaagaa
    10441 aacgattttt ggatttgcag cgctgatgtc tgctgtcgtt cttgcagcat gcgggggcgg
    10501 aggtacagat acctctggaa acaactctgg aggcaacggc aataacaaag aggaattaac
    10561 ttttccaatt gcaacaaaaa ataatgatga tgcaatcgaa gacggcgaat tagaagctgc
    10621 ggtcgcaacg gacagtcagt ttaaaggatt gttccagtgg gagttttacc aagattcata
    10681 tgatgcagct tttatggctc cttctcatga accgctattt gcaaacgatg acgatttcag
    10741 aatcacagac gacggagctg caacgcttga tctagatgaa gatacgaaaa aagcgacaat
    10801 caaagtaaaa gacaacgtca aatggtcaga tggtgaagat gtaacggcgg acgatgtgat
    10861 tttcccttac gaaatcattg ggaataaaga ttacactggt atccgttatg acgacacttt
    10921 ccgtaacatc gtaggtatgg aagaatatca tgatggaagt gccgacacaa tctccggaat
    10981 caaaaaaata gacgacaaaa cagttgaaat cgaatataaa gaagtaaacc caagcatgct
    11041 tcaatcgggt ggtggtatct ggtcatatgc agcaccaaaa catacgttag aaggtgtagc
    11101 aatcaaagat atggaatcaa gcgatcaagt acgtaaaaat ccagtgactt ttggccctta
    11161 ttacatgagt aatatcgttg caggagaatc agtagaatac ttgccaaacg agcactactg
    11221 gaatggtacg cctaaattga aaaagatcac gatgaaatct gtaccgatgg caagtgcaac
    11281 tgaagcattg aactctaaat tatatgatat gattttccaa atgccaacag atacttacga
    11341 tacctataaa gaaattgctg gctatacaaa cttaggccgt gaacaaactt cttatactta
    11401 tctaggattc aagttaggta aatggaatgc agatacaaag agtgtggaat acaatccaga
    11461 ttcaaaaatg gctgataaat cattacgtca agcaatgggc tatgcattag ataataatgc
    11521 agtaggtgaa cgtttctatg caggtttgag aagcaatgcg acttcattga ttcctccagt
    11581 attccagtca taccatgatg atgagatgaa aggattcaca caagatctag acaaagcgaa
    11641 aaaactatta gatgatgctg gctacaaaga tgtggatgac gatggtttaa gagaagacaa
    11701 agatggaaat aaactaacaa tcaactttgc ttcgatgtcc ggtggggaaa cagcgcaacc
    11761 attggcagat tactatatcc aacaatggaa agaaattggt ttggatgtcc aatacacaac
    11821 aggtcgtctg atcgaattca attctttcta tgataaatta gaaaacgatg atcctgaaat
    11881 tgatgtttac caagctgctt ggaatacagg aactgatcca aatccaagtg gactatgggg
    11941 accaaatgct gcgttcaact acactcgtta tgaatcagaa gaaaatacaa aattgatcaa
    12001 cgatatcagt tcaagcaaag catttgatga agagtaccaa aaagaagcat atcaaaaatg
    12061 gcaacaatat gcatttgaag aagcatttgc gattccaacg ttgttccgta atgaagtatt
    12121 gccagtcaac gaccgtgtca aagactggga ttggtcttat gatgtaccaa acccatgggc
    12181 aaatgtttct gttacagcag attcacgttc ataatcacta aaaatgaccg aaaaagggcg
    12241 atgtcctttt tcggtttttt gatatttttc tgtttatttc tctaatattc aggtcaaggc
    12301 gctgatttca tgacttctcc aataaacggt gttcaaagtc cagtttctcg gtattaagtc
    12361 aaatttatgc aaaaatatgg aaagccattt tcacaaaatt ttttcttcat actcgaaact
    12421 aaacgaattt ttcacaactt ctcgtcaatc actaggatta ttaaagcttt acttaccttt
    12481 tgaccgaacc atttcaaatt tgacaaaaaa ccagtataat aaggaagtat cgaaaaaata
    12541 tccataaaag gaatgtggaa aatggacaac cagctgacaa aagaactaaa agaaaactat
    12601 aacatcgttt ttcacgatct aaatttgctt gaacaagctt ttacccattc atcctatgtg
    12661 aatgagcatc gcaatctgca attatcggat aatgagcgac ttgaattttt aggagatgcc
    12721 gttttagaac tgatggtttc tcagtattta ttccgtttat atcctcattt gccagaagga
    12781 aaattgacaa aaacacgagc tgcgatcgtt cgcgaagaca gcttgtcgaa atttgccaaa
    12841 gaatgtcact ttgatcaata tgttttgttg ggcaagggag aagagaattc aggaggacgt
    12901 acgcgaccag cattgctttg cgacctattc gaagcttttt taggcgcgtt atatcttgac
    12961 cagggattcg acacgaccca tgcattcatc gaaaaagtga tttttcctaa agtcaaagca
    13021 ggtgcttttt cacatgagat ggatcataaa acaaaactac aagaagtctt gcaaaaatca
    13081 ggtgatgtat caatcgaata ccgattgatc aatgaagaag gtccagctca tgaacgagta
    13141 ttttggatcg aagtttatgt agatgatcaa ttgattggca ctggacaagg gaaatcgaaa
    13201 aaattagcag aacaagctgc ggcggaaaat gcattggcag cactgtaaaa gtaagtgagg
    13261 gaaagctttg tatttgaaac gaattgaaat agccggtttt aaatcatttg cagacaagac
    13321 agtcattgat tttgaaaata gcgtcacagc agtggttgga ccaaatggca gcggaaaaag
    13381 caatattaca gaggctatcc gctgggtatt aggagaacaa tctgctaaaa gtctgcgagg
    13441 cggaaaaatg ccggatatca tctttgccgg ttccgatacc cgtaagcagc tgaatattgc
    13501 agaagtcaca gtgattttag acaatagtga tcattatctg ccattggagt acaatgagat
    13561 cagtgtaacc cgccgttatc gacggactgg ggaaagcgag tttttcatca ataaacaaag
    13621 ctgcagatta aaagatatcc aagaattgtt cttagattct ggattaggaa aagaatcgtt
    13681 ctcaatcatt tctcaaggaa aagtagaagc gattttcagt agtaaaccgg aagaccgtcg
    13741 tgggatcttt gaagaagcag ctggtgtctt gaaatacaag cagcggaaaa agaaagcaga
    13801 acagaagctt tttgaaacag aagacaacct cagccgcgtc caagacatca tccacgaatt
    13861 ggaagaacag ttaactcctt tggccgccca gagtgaggca gcaaaggagt ttttacggct
    13921 aaaagaaaca ctgactcaaa ccgatgtctc tttgatggtt gctgaaatca agacagcaaa
    13981 aaaagattgg gacaacaaac aagcacagct tgcgaaattc aatctagaat taggcaaact
    14041 atctgagtcg atccaagaac aagaatccat tttggcgaag cagcgtaaag aaaatgcgca
    14101 agccgatcgt ctgatcgaaa aaaatcagca agtattatta gatctttcgg aaaaactgaa
    14161 gcagacagaa ggacaaaaag atgttttgca ggagcggaca aagcatacac aaaaaagctc
    14221 gcaagaatat caaacttcat tagcagaagc acaaaaaaag gttaaacatt ttgaaaagct
    14281 tcaagaaagc ttgatgaaag cagcagcaga aaaagaaaca gagatccaaa aagctgaagc
    14341 aaatctcata aaaacacagc aagaattaga aaaataccaa aaatcaacga aagaattatt
    14401 ggcagaatta agagatcaat atgttgattt gatgcaagaa caagcagctg taggaaacga
    14461 actgaagtat ctagaacgtc aatatataca agaaacagca aaaagcaagc agacgttagc
    14521 caagcaatcg gaagtagaag catcagtaga ccgattgatc ttacaaaaag aagaattgac
    14581 acaaaagcag gcgcagctaa aatcatcttt aactgaaaca aaagagaagt tagaaatgat
    14641 ccaacaaaac gggaaaaagt tccaagaagc attagcaaaa gaacagccta aaatgtatca
    14701 attaatgaat caagtccagc agctacgtgc tcgtaagaaa agccttcaag aaatccaaga
    14761 aaactatttt ggtttttacc aaggggttcg attggtcttg cagcataaac agcagctttc
    14821 gggtattgtc ggagcagtgg ctgaactgat cgatgttcct gcagatttca cgttagctat
    14881 cgaagcagct ttaggtgggg cagcccagca tgtcatcgtt gaaaatgaaa aagatgctcg
    14941 gcaagccatc acgtatttga aacagcaaag aggaggaaga gcaacattcc ttccactgac
    15001 gacaatcaaa ccaagacaat taccggctca tatattgaca caagcagcag ctgtagaagg
    15061 gtttataggg attgccagtg aacaagtcag ttatcccgat caaatccaaa ctgtggttca
    15121 aaacctgcta ggaacgatcc ttttagccaa agacttaact agtgccaatg caatcgcgca
    15181 aacgatccgc tatcaatatc gagtggtttc cttagaaggt gatgtgatga atgccggagg
    15241 atccatgaca ggtggagcga ataaacgagg aaaccaaggc agtttgtttg tacaaaatca
    15301 agaattgaaa cagttgacta gtgaatttga agaagcagat aaacaactcc aagcacaaga
    15361 aaaaaaagtt caggagctgc aacaagaaac agctcgttta gccgaagaac aagaagtttt
    15421 gcgtacacga ggcgaacaat tacgttttga agaacaagaa gcgacaaatc agctacaaaa
    15481 catcataaat gaacttgaac gttttgaaaa agaaaagcaa atctcaactt ttgagacacg
    15541 tgaactgcag cagtttatcg aagattacga aaagcaaacg aacgaattga aagacaaaca
    15601 aacagatctt gaatcgcaac gacaaaaaat cgacgaagaa atcaaatcgt taagtcaaga
    15661 aagcgaccag atggaagcca gaagagcaca agtacaaagt caaaaagcac aagaacaagc
    15721 agatcttgct gtcttgaaag agcagtttaa tcatctgcag atccagctcc gcggtgcccg
    15781 agtccaaaaa gcagaggcaa ccgaacgtca agaagcgata gaaaagcagt tggcgacatt
    15841 gacagcagac ttttctgatc atgaagtcac agaagaaagt ttggaaaaac aaattaacga
    15901 attatctgct cagcgagaaa cattaaaagc cgagttggca aaagccaaag agcagcgaga
    15961 tagaaggcaa aaggagatcg ataagcttga agcagtcttg gcagagcgta atcgtgagca
    16021 aaaagcacga ctgtcggaac aatccaaatt agaagtccaa aaagaccgag ctgaaatgat
    16081 tttagataat catttgacct atctgcagtc agaatatcag ctgacatttg agaaagcttc
    16141 ccaagactat caagaaacaa ccgatatcga agacagtaga acaaaggttt catccttaaa
    16201 agaacagatt gaaaaactgg gaccagtaaa cttaaattca atcgaacaat acgaacaagt
    16261 cagtgaaaga catacattct tagcaacaca gcgagatgat ctgttagcag cgaaaaacca
    16321 attgtttgaa acgatggatg agatggatga tgaagtgcgg acaagattca aagaagtatt
    16381 tgaagctatc cgtcaagaat tcaaaattgt ctttccaaat atgtttggcg gcggtaaagc
    16441 tgaattagta ctgacggaac caactgattt gctgaaaaca ggtattgaga ttgaagtaca
    16501 acctcctggc aaaaaactgc aaagtctaag tttgctttct ggaggagaac gagcacttac
    16561 agccatcgcg ttgctctttt ccattatccg tgtctgtcct gttccgtttt gtatcttaga
    16621 tgaggtagaa gcagcgttag atgaagccaa cgtgaaacgt tttggtcgtt acttgagcga
    16681 tttccaagat gacacacaat ttatcgtagt cacacaccgc aaaggaacga tggtcgcagc
    16741 agatgtactg tacggagtga cgatgcagga atccggtgta tcaaaaatcg tttctgtacg
    16801 gatggaagat atcaatgaag aggcgaaaat caacgaaacg aaagtgtagg aacgaaaatg
    16861 atcaaattag tagcaatcga tttggacggt acattattaa atgaacaaaa gctgatttca
    16921 gatgagaata aacaagcatt agcacaagca aaacagcaag gggtcaagat cgttctatgt
    16981 acaggacgcc ccttagctgc gatggcgcat tatctgcaag aattaggact ggtagatgaa
    17041 ggtgactaca gcatcacttt caacggcggt ttggtacaaa aaaatgatac tggtgaaatc
    17101 atggaaaaga aagtgatgga agtatcagat atccaacgct tatacaaact ggctcagcaa
    17161 ttggatctgc cactagatgt tttgtctgac aatgtggttt tacagctgcc tagtgcccca
    17221 aggaagcaat ccttgtacaa tgtgttgaat aagctgctgc aattccaacc agcggatata
    17281 agcgatatta cagatgaatt tgtgcttaat aaggcagtaa tcgcttatga ccaagaagaa
    17341 ttagatccta aaatcaaaga aataccagca gaattccatg aaatgtatga gatcatcaaa
    17401 acaagaagta tgcttttgga atttatgcca aaaggtgtta caaaagctta tggtatttct
    17461 ttattggcaa aagatcttgg tttgaaacaa gaagaaatca tggcgatcgg tgatgaggaa
    17521 aatgatttac cgatgatcca atatgctgga ctaggcgtag cgatggagaa tgctgtgcct
    17581 ttcgtcaaag aagcagcaga tttcgtgaca gccagcaatg aagaaaatgg cgtagccaaa
    17641 gtgatccaaa aatttgtatt agagtagggg gagtcaaatg ggattttttg ataaaatcaa
    17701 gcgtgctttt actggcgaag agaaaacaga agaagttgaa gtacaggaga agtacgacaa
    17761 gggtttagaa aagacgcgta aaacatttgg tgaacggatg aatgagttgt ttgccaattt
    17821 tcgaacagtc gatgaagact tttttgaaga attagaagaa acacttatcg gtgcagatgt
    17881 aggttttgaa acagcgatac gaatcaccga agcattacgt caagaagtga aactcagaaa
    17941 tgcgaaaaaa tcggcagaag tccaaaatgc gatcatcgag aaacttgtgg atctctatga
    18001 agcggaagga atcaatgaag taaatgaact aaatatccaa gaagacggac taacagttat
    18061 tttgtttgtc ggtgttaatg gtgtagggaa aacaacaagt attggtaaac ttgcccatca
    18121 gttcaaaaac gaaggtaaaa aagtattgat ggcagcagca gataccttcc gcgctggagc
    18181 aatcgaccag ttagttgtct ggggcgaacg cgctggtgtt gaagttgttc gtggcaatgc
    18241 aggaggcgat ccagcagctg tcgtattcga tgctttggaa agagcaaaga cagagcaggc
    18301 agatgtttta ctagttgata ctgcaggccg actgcaaaat aaagtgaact tgatgaacga
    18361 attagaaaaa ataaaacgag taatccagcg ggaattacca gaagcacccc aagaaacact
    18421 tcttgtatta gatgcaacca ctggtcaaaa cgcgatggtt caagcaaaac aattcaaaga
    18481 aacaacagat gtgaccggtc ttgttttgac aaaattagat ggtacagcaa agggcggtat
    18541 cgtacttgct atccgtaatg aattgcattt accagtgaaa ttggtcggtc ttggtgaagg
    18601 aatcgatgac ttggagccgt ttgatccaaa tgattttgtc gttggattgt tcaaaggatt
    18661 actaaaagaa gaataaccca tgtctaaaaa gcaggagctg tgacaaaagt tgcagctcct
    18721 tgtttgaatg attgttttta acatagctgt gtttttgatg ccttcatttt tatacttatt
    18781 tgtaagcggt tctcaaacaa tttcgttctt tttcttagac tttttgatta taattagatt
    18841 catatgaatt ggaggtcact taatgatgaa ttttaagcaa ttattattgc atgtcaatgc
    18901 gcgccctttt atcgaccaag ctcgatttgg tatagaacgg gaaggacagc gggttgatct
    18961 tgcaggaaat ctagcaaaaa ccgatcatcc agcaatcttt ggcgatcgat cctatcaccc
    19021 ctatatccaa acagatttta gcgaaacaca aacagagatg atcacccctg ttaccgattc
    19081 tattcccgaa ttatttcagt atctcgctgc tgtttatgat gtgactgctc gttctatacc
    19141 gaaagaagag atgatctggc cattaagtat gccacctgcc ttaccggaaa aagacgaaga
    19201 gatcattatt gcaaaattaa aaaatttcga agatgtcttg tatcgacgtt atttagcaaa
    19261 aaaatacggg aagcgaaaac agatggtaag cggtattcat ttcaattttg aatttggcga
    19321 tgagttgcta agaacattgt tcagccatca ggaagaattt caagattttt ccgaattcaa
    19381 aacagaactt tatttgaaaa cagcgagaaa ctttatgcgc tatcgctgga tgatcactta
    19441 tttattcggt gcttcaccaa tgagtgagaa gaactacttt ttagatgaat cacatccgca
    19501 agaacctgtt cgcagtattc gcaacagtgc gctgggctat acgaatcatc caaatgtgaa
    19561 agtttcctat gcttctatga aacagtattt agcagatatc gagcgaatga tcgaagaagg
    19621 gaaactttcg gaggagaaag aattttatac accgcttcgt ttccgaggcg gaaagaaagt
    19681 cgcagattta gcaacaacag gtgttcgtta tattgaattg cggaatatcg atttgaatcc
    19741 ttatgcaaga ttgggtatca atccagagca agttcggttc ttacaattat tcctgatgta
    19801 catgttatgg acagaagaaa aagaagactg tgaccaatgg gtagcagaag gtacgactcg
    19861 aaataacaaa gtagcactag aacaaccatc agatcaaaca gaatttcatc aagaaggtag
    19921 agaaattctt gaaggaatga agcaaatgct ggttgaattg gattggttgg attctcttta
    19981 tttagtagag gaagcattga ctcaaatgga tcatcctgaa caaacgttag cagcgaaact
    20041 ctatcaagaa gcacagctat cgagtcagca agaagttgct gtggcattgg gacatcaata
    20101 ttataaagaa agccatgaac gtccctatca gttggccggt tttcgcgaaa tggaactttc
    20161 tactcagata tttatgttcg atgccatcca aaaaggtgtc caagtcaaag tattagatga
    20221 gtcggatcaa tttttgcgtc tgcaattcca agaccatgta gaatatgtga aaaatgcaaa
    20281 catgacaagt aaggacagct atatcgtccc acttatcatg gaaaacaaaa cagtaacgaa
    20341 aaaagtctta aaagaagctg ggttccgggt accaggaggc gcagaatttt catccatgga
    20401 agaagcggta aaagcttatc cgagatttgc cgagcaggca tttgtgatca aacctaaatc
    20461 aaccaattac gggttaggta tcacgatttt caaggaagga gctagtctgg aagattatca
    20521 agcaggttta gccattgctt ttcgtgaaga cagttcggtt ttagtagaag aatttatgcc
    20581 gggaacggaa tatcgtttct ttgtgatcga cggagaagtc caagccatca tgttgcgagt
    20641 ccctgctaat gttattggtg acagtatccg aacagtgaaa gaacttgttg aagaaaaaaa
    20701 tagtgaccct ttgcgtggaa cgaatcatcg tgcaccttta gaattgatcc aattaggtga
    20761 attagaacaa ttgatgttga aagaacaagg gttgacgatc gaatcagttc cccaagccaa
    20821 tcaaattgtt tatctaaggg agaactcaaa tatcagtaca ggtggtgatt cgatcgatat
    20881 gactgatgaa ttttctgaag cttacaaaaa aatcgccgtt tctgcggtag aagcactagg
    20941 agctaagatt agtggtatcg atttgattat accagataaa gaaatcgatc ctacaactga
    21001 taaaaaagca tatgggatca tcgaagcaaa tttcaatcca gccatgcata tgcatgttta
    21061 tccttttgca ggaaaaggca gaagattgac gatgaatgta ttgaaattgt tatatcctga
    21121 agtattttaa aagaaaaagc ttttgacaga ttgtcctatt tgatgtcaat ctgatcaaaa
    21181 gctttttttg catgtaaaaa tttatgtgtt ttttcgtttt aaaataaacg atcccagaag
    21241 ctttttttct tttttggttt ttctttttca gctggcgaag agttttcttc tgggtatttt
    21301 ttttcgatat caatcacagg ttcatttact gcttcttttg ctgttaagag caatccgaat
    21361 tcctcaggat ttgcagtgac gtaatcgttg actacagtga aacggctatt gtttttcgtt
    21421 gctagctgga tatattggtt ttggacagaa aggggtagag ccccattcaa gagtatggaa
    21481 gcatctggat gttttttgag ttcttttaaa aagttttctt tgttttttgg ttggatcatt
    21541 tcagcaacag tcatactaag atagcagcgt tccctgaatg tccctaaata tttatgctgt
    21601 tcttcagggt tcactaaagg tgtaccataa agtcccttat ccaaatggct ttttacatca
    21661 tcttgtgcca taaaaaatac cctccttctt ctctttgtct ttcttacgat tatagcatat
    21721 tcccaataat acctaaaaca tatgaaaaag cggttgcttt tgttcaacga ttcacttaaa
    21781 ataagaatga aaatcattct caattagaaa ggtggattgg atgcgctacg gacgactagt
    21841 agcattatta gttattttaa gtagtatttc attgttcatc ggagtatcga atgtacaatt
    21901 gtcagaactg ctccagttag atgggcaaag cttacaaatc ttgctagtga ctcgtttgcc
    21961 acgaacgatt tgtttgatcc ttgtgggagc gaccagtagt atttgtgggt tgatcatgca
    22021 acatttgacc caaaataaat ttgtttcacc aacgactgcg ggcacgatgg atagtgcgcg
    22081 tttaggtatt ttagtggcta tgattttcct acctggtgca tccttgattg tccgatcctt
    22141 gacagcattt tgctttgctt ttgcaggaac attgcttttt ttatccctta cgcgattgtt
    22201 cctccagaaa aatcaattga tgcttccgtt gattggagtc atgttaggga atatcattgg
    22261 ttcagttgct actttttttg cttatcaatt ccagttggta caaaatatga cttcatggct
    22321 tcaagggaat tttgcgactg tgatacgagg aaattatgag ttattgtatg ctgttgttcc
    22381 attgttgctc ttggtttggc tatttgctta ccgatttacg atcattggtc tgggcgaatc
    22441 gtttgcagta aatctgggaa tagattacca gaagatgcag tttttaggca ttggcttagt
    22501 atcattggct agtgcagtca tgttgatcat ggtaggaagc attcctttta tcggagtgat
    22561 cattccgaat cttgtctcta gaatatatgg agatcaagtg cacaaaacaa ttggcgtaac
    22621 tgctttagca ggtagtttgt tcttagtatt ttgcgatctt gtcgctcgcg tgttgatttt
    22681 tccttatgaa ataccagtca gtgtgatcgt tggaatcatc ggtggggcta tcttccttta
    22741 tcttctgata aggggacgac gatatgctta aaaaaaagcc atctattttt attggtatgc
    22801 ttgttcttgt tttatttgtt acaggaatgt acttgacgat ccatacatat ggaaactggc
    22861 attttgcatg tgcattgagg agtaagaaaa tttttgcatt tatccttgtc tcgctagctg
    22921 tcagcagtgc cacaatcagc tttcagacaa ttacgcataa ccagtttttg actccttcag
    22981 tattgggact tgagtcgtta tatgtccttg tccaaacggt ccttttcttc tttatcggag
    23041 gagtgacgat gcttcagcag acaggtacag tttttttctt attgaacgtt gggctgatgg
    23101 tcgggctgag tctattgtta tttttctttc ttttgaaaaa aatgagagcg aatctttttt
    23161 tattactgat gtcagggatg attttaggta cgctttttgg tagtgtcagt acctttttac
    23221 aagtcatcat ggatccgaat gaatatgatt tgcttcaagg cagattattt gcaagctttg
    23281 gcaatatcca gagtcagtat cttttgcctg cttctttgat cattatcggc tgctggtacc
    23341 tgttatttcg atatcgacat gtgctagacg ttttccatct cggaaatgaa caagcagtaa
    23401 acctcgggat agacacagat cgttttgaat ttttgatttt atgttgtgtc agtcttttga
    23461 cagggacatc aacggctctt gtgggtccag ttactttttt agggtttatt gtcgcaaata
    23521 tcagttatcg tgtcttttct acttatcgtc atggtcagct atttatcgga agctttctgg
    23581 tagctatgct gtttctgaca ggaggacagc tcgcagtaga gcagattttc ggctggaata
    23641 caacagtgag tgtcgtcgtt gaatttgtcg gaggaattta ttttgtcaca aaattgattc
    23701 gcgaaaggaa gagtatccta tgattgattt acaaaaggtc aataaactgt accatggaaa
    23761 atcagtggtt aaagatgtac agttaacgat tcctgaaaag aagttgactg cttttatcgg
    23821 tccaaatggt gctggaaagt caacggtgtt gtcgatgatc agtcgtttga taccaaaaga
    23881 tacaggtgaa atctatttag accataacga agtaaaggct tggaaatcca atgaattagc
    23941 caaaaaatta tcgatcctac gacaatcgaa tgaaataaat gtccaaatca ccgtgaaaga
    24001 attagtagaa tttggacgat ttccttatac gaaaggaaaa ttgacgacaa aagacgagga
    24061 aatcgttcag gaatcactgg aacaccttgg gttagaagaa atagcggatc aatcgattga
    24121 cactctttca ggcggtcaac tccaacgtgc gtatattgca atggtgctag cgcaagacac
    24181 ggattatatt ttactggatg aacctttgaa caatctagat atgaattttg cggttcaaat
    24241 catgcagatt ttaaaaaatt tagtacaaga aatcgggaaa acaattgtta ttgttcttca
    24301 tgatatcaac tttgctgcaa gttatgcaga cgaaattgtt gcaatgaaag atggacagtt
    24361 atttacgcaa ggaacgaccc aagaaatgat aaaaaaagat gtattggatg aattatacga
    24421 aatggacata cggatttgtg agattgaagg ccgcagattc tgtctttatt tctaaaacta
    24481 acattaggag agaagagatg aaaaaattag tagtaggtat cttgattcta gcatcatttg
    24541 gtttagcagc atgtggaaat agcaacaaca ccagtcaggc agacacaaaa aaaagcagca
    24601 cacaaactga aactacattg acgataacag atagtaatgg agaccaaatc gaggtgccaa
    24661 ataatccaga aaaagtcgtc gtattcgaca atggttcttt agatacgatg gatgctcttg
    24721 gtgttggaga taaggtagtt ggagcagcta caagcagcct tcctgagtat ctttcatcct
    24781 ataaaaaagt agaatcagca ggtggaataa aagaaccaga cttagaaaaa attaatcagc
    24841 ttcagccaga tctaatcatc atttctggcc gacaaagaga ttttcaaaaa gatctaagtc
    24901 aaattgctcc gaccattttc ctttctttag atgcgaaaaa tccatgggaa tctttccagc
    24961 aaaatgtcac tgctttaggg gagatctttg gtaagcaaga agaagctaaa actcaattag
    25021 aggaattatc tagtgcaatc gatcagacga aaaagaaagc agaagctact gacaaaaaag
    25081 cactagtcac acttgtgaat gaagggcaat tatcagccta cggatctggt tctagattcg
    25141 gtttcatcca tgatttattt ggctttgaac aagcagatga tcaaatcgaa gcatccactc
    25201 acggtcaaag tgtttcttat gaatatgtgc tggaaaaaaa tccggatatc ttatttgtag
    25261 tagacagaac aaaagctatt ggtggagacg acagcaaaga tgatatttct gccaatgaac
    25321 tggtagcaca aacgaatgct ggtaaaaatc agcagatcat ctcccttgaa ccagatgtct
    25381 ggtatttaag cgggggcggt ttagaatcaa tgaaactgat gatcgaagat gtgaatcaag
    25441 catttaaata gcataaataa cagaggatta aactctcata ctatgtaaca ataatgtttc
    25501 ttacattctt aaatagcaag agcctatcct caaagaagtt atcaaaaacc atccgtaaaa
    25561 tcgtattgat gattttacgg atggttttta tttatttgag aaatcttttt tcaacgtgaa
    25621 ttcattatct acatcatacg ggttgattct agaatgggga gtggcaaaaa atagtgtgat
    25681 ccattttttc agcatcaaga tcgcctcctt tgatttgaat caaaggtaca aagcgaaact
    25741 tagataaaac ttaaatttag atatttttat tttgtaccga tctcttcttt aaacgatgtt
    25801 ccatctgctg cttctcggca ataagtccac tagactagaa aatatgagaa gctattttct
    25861 gtccagcgtt cttattactc aaaacaaatc gcagttgtca caatctcttc taaacgatgt
    25921 tccatctgct gcttctcggc aataagccca ctagactaga aaatatgaga agctattttc
    25981 tgtccagcgt tcttattact caaagcaaat cgcagttgtc acaatctctt ctaaacaatg
    26041 ttcaatcagc tgaccttcgg tattagctca aagcactgat ttcacaacct ctatttaggc
    26101 atgtgtctca gcaagcggtt gatagttgat gatctgcatt tttcgataaa aatcggcggg
    26161 aagcttactc caccgtttta gtaattgata gaaataactc tcttctgctt tgattttata
    26221 ctcgcttaat atcaatccaa aactattcgt tttttttgaa gctaattttt tataaatatc
    26281 caatgctttg gttttatctt cattgacaaa gagtgttttt acttcaatat aagaaccctc
    26341 gatattttgt gtttcttcga taaaatagat gttttttata tattccttca tagtgattcc
    26401 tccaaagaag tcttttattt gataagttta ctctaccgaa gagttgttaa taatccgtga
    26461 acaaaaagta attctttttc caatagttgt taaaggtttt ttatttttta tagtattttt
    26521 tgttatttaa gaaaggaata aggttaaaca aataacaggt tgctttttgg gtctttttcc
    26581 gttcaagaaa ctgaacgaag aaaagggaaa gttgtctctt ttgtgataag agaaatcata
    26641 ataaacgaat gacttacttt ccaataaatt gagaacagtc agaaccagac aaattagttg
    26701 catctgatga ataaagtgat tattctatga catgaggtga tacagatgaa attagaagtg
    26761 acaaaagaag cacaagaagt attgaagaat aaattagaag cagacgatca acttttatta
    26821 gatatcgaaa acggtgacgg tccatttgca gaaagccaag tcagttgcca attggatacg
    26881 gcatttcgat tgatcattgt caaaaaagat acacaaacag atctaagtct ttattctgta
    26941 gtggctgata caccagttgg accaatcaaa atcaaagaca gtgcaatact ttacatggat
    27001 gatccatcca cacttgcttt agaaccaact tataatagtt tacaattaaa aggaccaagc
    27061 ggcttgagaa aaggcaactt gcaagttgtt cgaaaagact agagggataa aatgggaaaa
    27121 ttggcgatca tcagcgacct acatgtggac atcaataaat tcggcgaatc agagctgatg
    27181 cagctttggc aagttttgca aaagcacaag atcactcggc ttcatctagc cggagatacg
    27241 gctaataaag tagaccgttg cctagctgtt gtcgaatttt tcgtcaataa aggattatct
    27301 acgacatttc atttcggcaa tcacgagttg gcagatgtgc ccaatgaaca gatgatggac
    27361 cattttccag acccacattt tttaaatgaa cggtatattg ctttaaatga gcagaccgtt
    27421 cttttaggga tgaatggctg gtacgactat cagttttccg aattgaaaga ttacgaggca
    27481 atcgtacgaa tgaagcgttt gtattggtat gatcggttca tccagcgtgg gaaaacagat
    27541 ctcgaggtca atgcagaaat gcttgcagct gcaaaaaatt tgctagacca gctggaagca
    27601 aaaaaacttg aggtgatttt atccactcat ttcgttccta aacgggcatt tatcatgtat
    27661 caatctgctc cttatgagcg ttggaacaaa ttaaatgcat ttttaggatc agaatcattc
    27721 ggagaattac ttgaccgtta ttcaaatatc aaacaagtag tttttggcca tacgcatcgt
    27781 cgctttgaag ataaatccat cggtcacacg atttacagct gcagaccttt tggttattat
    27841 tatgaatggc agctgacccg agattttgtc ttgaaagaac agttgctaga cagctatgat
    27901 cctatgaaat tacgtgtcct cttgcgtgcc catcacccag cttttcaggc ctatcaaaca
    27961 caacatttgc aagaggaatt tgaacaagcg atgacaatcg ttccgtattg agacagcatt
    28021 agtaatgtgt aaaagaaaat accttgtaaa acataaggat aagtaagagc agtagatcca
    28081 tttatcgtgg atatgctgct ttttttgcgg aaaataatca gcgaatgttt atttgatcgg
    28141 atgatttgta actcgcagag tactagatgc aagcgtgtat tctttttttg aacgataatc
    28201 acaaaaaaaa atataatata cattttttat ttaattataa ttaatattga ttgaagtcta
    28261 ggcagtcaag tcacattttg atagatagga gggggactta cgaattattt taaaggctat
    28321 gacagctgta tttctagatt gtgtttgcat caagttgcaa taaaaactag aaatcaaatc
    28381 gggctgataa tcatttataa ctaatcgata cataggacca aatataggtc tatgttcttt
    28441 tatttcataa gtatcgtaaa acagaatttt gctatggatt aatccaatcg actgatatgt
    28501 ttagtaaata aagaaagagg aatgtcaatg atgaaaaaag aaatcgtatt aacagcagca
    28561 atgatgttat tcagcatggt agcaagtacg acttttgttt ctgcaactga agtataccca
    28621 aaggaatata acacagaagg aacgattaca tttgaagcag gagatgaggg tgtaactcct
    28681 cctgttgatc cagagaatcc tgatcccaat aaatctgtcg atccatcaga tcctccaagc
    28741 cctggaacag gaggggcatt atcgattgat tatggatcaa aattcaaatt tgggactcaa
    28801 aaaatttcta ctgcggataa aacgtattat gcagctgctg atgtgatgaa cgatgggtct
    28861 agaaaaccga catatgtcca agtgacagat aggagaagta ccttatctgg gtggaaatta
    28921 agtgtctctc aaccagaaca gtttaaaact gccagtgggg atgaactagt tggagcacag
    28981 ctgaaattta cgaaagggca ggctgtttct ttggttgatc caacatatac gccgcaaacg
    29041 gtgaattctg aattaacgtt gacaccggga ggaaacaata cgttagcaat taatgctaaa
    29101 tcaggaacag gtgtggggac ttgggtctat cgatttggtg caaatgaaaa tgaaaatcaa
    29161 gatgcggtac agctttcggt acccggtaaa tctgtcaagt tagcgcaaca atattccact
    29221 aaattagttt ggacattaga agatacacct aacaactagt ttactgaaaa gaactgttaa
    29281 aaataaatac gtagtcatat tgtcttgcga ggtaatcgag caaaaattgt caaaggctag
    29341 gctaaatttg tttggatacg aatagttcat ctaggaattt aacgatcagg aggaaaagat
    29401 atattgaaaa agaggaggat cgtgctcatt ttaagcagtc tcttagtgac taatatcttt
    29461 ccgatcatag ggtactcttt ggaattgaat cagttggagc atggaaatag aagagacgaa
    29521 gaaagcaaac tcacttcgac attcgacagt ttaatagaat ccaaccagac acaagaaagc
    29581 agtgaggatt taagtatttc gagtagtgga gagaccactg acaacgtaga agctgagggt
    29641 tcggacaaac aaggactaga agatgcacca atttctgcgg ttgagctgcc tgacgatccc
    29701 aatattattt ctatcgataa aatttttcaa acacctatcg gtactagcac gtccatattg
    29761 gaaaatggga agttactgca gttgaaccca gcagaaaggt ctcaaagagg agctatttgg
    29821 tcaaataaga agattagttt actctcggat tttacattta aatcttattt atacttagga
    29881 aatcaacgag atagagcggg agatggcatg acctttacat tgactggcga tcctagaatg
    29941 gctagtaatc ccagtcaagt tattggatcg ccaggtatgg gaattggctc atattctact
    30001 cgttctggcc aaccttacat tgcgaatgcg ctctcgattg agtttgatac gtataaaaat
    30061 agtggttctt ctgatcgaat ggatcgagag ataactgctg ataatagagg gcatggacat
    30121 gtcgcttttg tcacgcctaa accaaataat aataattatt ctggagagca tactggcgtg
    30181 acggtagctc ctacatttct ttctaatggc acatggcgct tgttaataat taaatgggac
    30241 gcggagcaaa gaagtcttac ttatgatttg gaaggtgtcg gtcgctcttc tcggattata
    30301 cctgatctca ttaggcagtt tggttctaca gaagtaaatt gggggtttac ttcttcaaca
    30361 ggtacttttt ttcaagaaaa tgctgtcgct gtcacgcgac ttccatcgaa tgtcacatca
    30421 acagctgatg taaaagtcaa cgatagtgat tatggggcca tcgctgaagc tggaggaaac
    30481 gataagataa ctatcagaaa tacattgaag gtcgaggggg attttcttga atataagaaa
    30541 atgcagacta gtatcgacct cccacaagaa ttaactattc ccgagcatat cttgatcaat
    30601 ggaaatcaaa ttgatgccaa agatatcttg ataaccggaa atcgagtcac gctaaatctg
    30661 acagattatt ttattgctgg agatacgtta ataatcgaat tggagacaac tttgcaggaa
    30721 gtaattcctg aaaaagtact cactatgcat tttgaatacc ttgaaaacgg gaatttgatt
    30781 gagcaatcga atgaaataaa cctaaagatc gcaaagctga aggagataga tatccatgtt
    30841 tattataaag ataaagacag tcagattgat attgctgaaa tgaaaacaat cactgggaaa
    30901 ataggagaga agtataaaga aactcctatt gatattgaag ggtatgtttt tgactcggat
    30961 tcagcgaata cagaaggcat cttctccgaa aatacagagg atatctattt ttattacaga
    31021 aaaggcaaac tttatctctc aactgctcct caatatgtca attttggaaa acataaaata
    31081 tccagtagac cattaagtga attcggacag ttttcaggtg ggttgaaaat tatagatgaa
    31141 cgggcttttg gaagttggaa attacaattg aaacaagaga aagttcttac aaatggagat
    31201 tttgagcttc cagaagctct ttcctttgtt acctctgaaa atacaacggt tatagggaca
    31261 tccgctgtta cgattttcga aagtgatcaa aagggtgaaa gcgatttgtc ctctctttta
    31321 gattctacag ctcatcgtgg aatcagaata aatattcctg tagaaaacca aagaatcgga
    31381 acctttgagg ggaaactatc ttggatactt gcagatgtcc caggaaatta acaggaagag
    31441 aagaatataa caaaattatt gagtttcaag aagggcacag gtaccatagg ctcttgtgtc
    31501 ccacagagac aagattttgt tatagtcttc tcttacatat gattaaagga gcgattttct
    31561 tgagaccaaa acaacaatta tttataacac tcctagttat tttaggggca ctttttaaca
    31621 atatatcagt gtcagctagt gcatttaatt tttcagtgac gccgataact tcagagaatc
    31681 aaatcgacaa gagaaaaact tacttcgatc ttcaacttga tccggatcag gaagtagaag
    31741 taaaagcgga attgagaaat gatacagaaa aagaagtgaa aatcgatatt tctgtgaata
    31801 gcgcaaccac caattcaaat gtgatggttg aatacggaaa aaacgagatt gaaaaagaca
    31861 tgagcctgat ctttgatctg gtagattatg tgagctatcc acagatagtg acgctaaaac
    31921 ctaaaagtgt ccaaaccgtt gcttttcatg tcaggatgcc gaatgagcga tttgatggtg
    31981 tattggctgg tgggatcaca ttccaagaaa tccagacgga aaaggaccaa acagaaacta
    32041 aggatcaaag cttatcgatt gagaatgaat acacctatat tattgctttg ttgatgcaac
    32101 agacactgaa cgaagttgct ccgaacctta ctcttcatga agttaaacca gatcaaatca
    32161 atgcaagaaa tgtcatctta gcaaacgttc aaaatgatca aaaaacctat atcaatcaag
    32221 tagttattga aacaaaaatt acaaaaaaag gacattctga agttttatat caagaggaaa
    32281 aagaaggatt gcagattgcg ccgaatacaa atttttcttt tccaactgct ttaaatggtc
    32341 aaccgctaac cccaggtgaa tatcacctta caatgactgt tttaggaaat gaaaatgaaa
    32401 atggaaaatt ttcgagaaaa aaaggaaata ctacgatcaa ctataccaat caatgggtat
    32461 ttgaaaaaga ttttacaata gacggaaaag tagccaagga attgaacacg acggatgtta
    32521 cgattaaaaa agacaataat tggat
//
LOCUS       DAYYME010000017        32272 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000017 DAYYME010000000
VERSION     DAYYME010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.017"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(477..2369)
                     /locus_tag="ACHBFW_000650"
     CDS             complement(477..2369)
                     /locus_tag="ACHBFW_000650"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9316991.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2366..4093)
                     /locus_tag="ACHBFW_000651"
     CDS             complement(2366..4093)
                     /locus_tag="ACHBFW_000651"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9316992.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(4109..4759)
                     /locus_tag="ACHBFW_000652"
     CDS             complement(4109..4759)
                     /locus_tag="ACHBFW_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFM9316993.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4963..5259
                     /locus_tag="ACHBFW_000653"
     CDS             4963..5259
                     /locus_tag="ACHBFW_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM9316994.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5451..6071
                     /locus_tag="ACHBFW_000654"
     CDS             5451..6071
                     /locus_tag="ACHBFW_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM9316995.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6163..7329)
                     /locus_tag="ACHBFW_000655"
     CDS             complement(6163..7329)
                     /locus_tag="ACHBFW_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9316996.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7341..7694)
                     /locus_tag="ACHBFW_000656"
     CDS             complement(7341..7694)
                     /locus_tag="ACHBFW_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM9316997.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7822..8844
                     /locus_tag="ACHBFW_000657"
     CDS             7822..8844
                     /locus_tag="ACHBFW_000657"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFM9316998.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8863..9024
                     /locus_tag="ACHBFW_000658"
     CDS             8863..9024
                     /locus_tag="ACHBFW_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9316999.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9283..9717)
                     /locus_tag="ACHBFW_000659"
     CDS             complement(9283..9717)
                     /locus_tag="ACHBFW_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317000.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9827..11686)
                     /locus_tag="ACHBFW_000660"
     CDS             complement(9827..11686)
                     /locus_tag="ACHBFW_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM9317001.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11687..11827
                     /locus_tag="ACHBFW_000661"
     CDS             11687..11827
                     /locus_tag="ACHBFW_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFM9317002.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11804..12968)
                     /locus_tag="ACHBFW_000662"
                     /pseudo
     CDS             complement(11804..12968)
                     /locus_tag="ACHBFW_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(13275..14699)
                     /gene="araA"
                     /locus_tag="ACHBFW_000663"
     CDS             complement(13275..14699)
                     /gene="araA"
                     /locus_tag="ACHBFW_000663"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFM9317003.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14715..15413)
                     /locus_tag="ACHBFW_000664"
     CDS             complement(14715..15413)
                     /locus_tag="ACHBFW_000664"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM9317004.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15370..17031)
                     /locus_tag="ACHBFW_000665"
     CDS             complement(15370..17031)
                     /locus_tag="ACHBFW_000665"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFM9317005.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17249..18649
                     /locus_tag="ACHBFW_000666"
     CDS             17249..18649
                     /locus_tag="ACHBFW_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFM9317006.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18901..19980)
                     /locus_tag="ACHBFW_000667"
     CDS             complement(18901..19980)
                     /locus_tag="ACHBFW_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9317007.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(20066..21025)
                     /locus_tag="ACHBFW_000668"
     CDS             complement(20066..21025)
                     /locus_tag="ACHBFW_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM9317008.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSKKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(21037..22548)
                     /locus_tag="ACHBFW_000669"
     CDS             complement(21037..22548)
                     /locus_tag="ACHBFW_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFM9317009.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22570..23133)
                     /locus_tag="ACHBFW_000670"
     CDS             complement(22570..23133)
                     /locus_tag="ACHBFW_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM9317010.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(23139..24002)
                     /locus_tag="ACHBFW_000671"
     CDS             complement(23139..24002)
                     /locus_tag="ACHBFW_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9317011.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(24016..24912)
                     /locus_tag="ACHBFW_000672"
     CDS             complement(24016..24912)
                     /locus_tag="ACHBFW_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9317012.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(25087..26376)
                     /locus_tag="ACHBFW_000673"
     CDS             complement(25087..26376)
                     /locus_tag="ACHBFW_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9317013.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26348..27865)
                     /locus_tag="ACHBFW_000674"
     CDS             complement(26348..27865)
                     /locus_tag="ACHBFW_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFM9317014.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            28063..28953
                     /locus_tag="ACHBFW_000675"
     CDS             28063..28953
                     /locus_tag="ACHBFW_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFM9317015.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(29026..29850)
                     /gene="nadE"
                     /locus_tag="ACHBFW_000676"
     CDS             complement(29026..29850)
                     /gene="nadE"
                     /locus_tag="ACHBFW_000676"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFM9317016.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29862..31334)
                     /locus_tag="ACHBFW_000677"
     CDS             complement(29862..31334)
                     /locus_tag="ACHBFW_000677"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFM9317017.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31971..>32272)
                     /locus_tag="ACHBFW_000678"
     CDS             complement(31971..>32272)
                     /locus_tag="ACHBFW_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM9317018.1"
                     /translation="VEFFFGNTAINYYKLFSLDRTQLLLVDTYDKKQVVMINTKKKIT
                     RQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFKRPNKDIVVIEQKNTKDSI"
BASE COUNT         9437 a         6744 c         5594 g        10497 t
ORIGIN      
        1 gggcttatta ccgcagatgc aagcttttga acaccgttta aggaagaggt tgtgaaaaag
       61 tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg gctttctaca tttttgcata
      121 aatttggctt aataccgaga aactggcttt tgaacaccgt ttaccaagag gttgtgagcc
      181 tgccgtttaa ctccgagtca caaggagcaa tttcaaaaaa cagcttctca ggtttgttga
      241 ctattgtgac ttgtgaccga ggagttggca ggagaacacc gtttaaggaa gaggttgtaa
      301 aatcagcggt ttgacctgag tattagagat acaaaacgga aaaatagctt ctcatatttt
      361 gccgtttttt gagctaatac cgaaggacag ctgattgaac accgtttatt cggaaaaagg
      421 gactgtgaca cgacttttgt cacagcccct aaaaaaaata ataaatttgt actattctat
      481 gaagcttctt cttcagaaaa ctgactgtta taaagatctg catagaatcc gtctttttcc
      541 atcaatgtat cgtggtttcc tgtttctacg atcgaaccat gattcatcac aataatgttg
      601 tctgcatcgc gtattgtaga caagcggtga gcaacaacga aacttgtccg gttttccaat
      661 aggcggttca tcgctttttg aatcagtatt tctgttcgtg tatctacact agatgttgct
      721 tcatcaagga tcaggacatc tggatttgct aagaatgccc gcgcaattgt gattagctga
      781 cgctgccctt gcgaaatatt gcttgcatcc tcgtttaata cagtgttata tccttctggc
      841 aatttacgaa caaaatcgtc tacgtgagca gctttagcag cctcaatgat ttgttcgtct
      901 gttgcatcgt cgttcccata tttgatattg tcatagatac ttcctgtaaa cagccaagta
      961 tcttgcagta ccattgaaaa ctgtgcgcgc aattcatcac gtgaaagatc acgggtatct
     1021 acaccatcgt aacgaatact acctccagaa acatcgtaga accgttcaag aaggttgatc
     1081 agtgtcgtct tccctgcacc agtcggacct acaatagcga ccatctcacc aggtttgaca
     1141 tccaagttaa aatcagtcat tagcaagtta tctggtgtgt agccgaattg aacattttca
     1201 aaacgaactt tgtatggtgt atctttttct tctggaaggc cagatttttc attggacatc
     1261 tcttcttcat ccaacacttc aaatacccgt tcggctgaag caactgttga ttggatagtg
     1321 ttcatcaaac ttgcaagttg agtgattggt tgagagaact gtgttgtata ttgcaagaac
     1381 gcttgaacat cccctaatgt catgttccca tttgcaactt ttactccgcc tagtacagcg
     1441 acgaacacat agcctagatt tttaacgaaa ttcatcaacg gcatgatgat tgccgaaata
     1501 aattgagctt tccaaccagc ggcatacaga ttctgatttt ctttttcaaa tacttcctgg
     1561 tctttttcag tatgattgaa tgttttaaca atggtatgac caccatatgt ttcttctact
     1621 tggttattca ataaacctaa acttttttgc tgatctgcga aaaacacttg tgatttcggc
     1681 gctacgatca tcacgatgat caagcttaaa gggatcatca ataacgcgac taacgtcaat
     1741 tgccagctga ttgaaagcat catcacgatg accccaatca atgtgactgc actagttaca
     1801 aattgagtca aattttgctg cagcgtgctt gcgatgttat ccatatcatt gattgctcga
     1861 gacatgatat ccccatttgt atgtgtatca taataggaaa tcggtacacg attcattttg
     1921 gcttccaaat catgacgtaa ttcataaact gttcgttggg aaacacgtgt cataataaat
     1981 tgctgtaaaa agttgaatac agcagaaatc aaatacatca cgattactgt cgcaatgatc
     2041 tgcgcgattt tatcaaagtc tatcggaaat tttgttactt gttggccttg ttgcatctgt
     2101 tgagcgccag ccattacacc tttgaatatt tctgtggttg ctttccccaa gacctttggt
     2161 gtttggattt ggaagatcac ggctgctatt gctagtacaa atacagcgat gatcgcaacc
     2221 atacgtttag acatatagcc aaacaatcgg cgtaccgttc cccagaaatt tttaggcttt
     2281 tcacctttta caccgagatt tctgccaggt ccaccatgtc ctggaccgcc tgctggtcca
     2341 tgagagggac gtgtgtttgt tttactcatg ctaagtcctc ctctcttagt tgtgaatgaa
     2401 cgatttcttg ataagtttcg tttgttgcta atagttcttc atgtgttcct ttgccaacca
     2461 gttttccttc atctagcaca aggatcatat ctgcatccat tactgtactt actcgttgag
     2521 caactaaaac ggtgatactt tctttcatat tcgttttcaa tgcttggcga agattggcat
     2581 ccgttttgaa atccaacgct gagaaggaat catcaaacac atagatatct gctgttttga
     2641 ctaatgcccg tgcaattgcc agacgctgtc tttgtccacc agagaagttt cctcctcctt
     2701 gttccacatg gctatctaag ccatcttcca tctctgaaac aaaatcttta gcttgtgcga
     2761 tttctaatgc tttccaaatc atttcatctg ttgcatccgg cttaccatac tgcatattgt
     2821 ctcggatcgt tccagagaac agaactgctt tttgaggaac aaagcccatt aattctcgca
     2881 aattatattg agtcatgtca ctaatgtccg ttccatttat ttggatcgaa ccagattcga
     2941 tgtcatataa tcgagggatc aaattgacta aggtcgtttt acctgaacca gtccccccta
     3001 tgattgcgac gatctcgccc gacttggctt ggaaatcaat atcttccaat gcaagttttt
     3061 ctgctccatg atatcgataa ttgacatgat tgaaggccaa tgttgcgttt tttccagcaa
     3121 aagaaactgt tttaggattt tcagggtcat ggataccgat tttttcatcc aacacttcat
     3181 taatacggtc agctgaagct tgtgcacgag gcaccatgat aaaaatagcg gaaagcatca
     3241 taaagctgat caagatctgc atcgcataag tcataaatgc aatcagatta ccgacttcca
     3301 aagtcatatc tgctatgtag tgtccaccaa accaagtgat tgctacgttt gtaccgctca
     3361 tgatcaatgt catcattggc atcatcaatg cgacgatcgt attaactttg attgccgtat
     3421 ttgtataatc tttattcgct aaatcaaaac gattttcttc aaagcgtgtc ttatcgaatg
     3481 ctcgaatcac tctgacccct gtcaatcctt ctcgaaatac caggtttaaa cggtcagttt
     3541 tcttttgcat acttttgaat aatggaacag caaagtacat gattccgccg atcaaaataa
     3601 tcattactgg gatgacaact aagaagattt ttgtcaactg atgatctttt tgataagcaa
     3661 gtatgctcgc gccaatcaac gtgattggtg cattgatcat catacgtaaa aacatttgtg
     3721 taaccatttg gatttgagtc acatcattgg tcgttcttgt gatcaaagag gccgttccat
     3781 atttatcaaa cgcatctttt gtcagactct ctgatttttt atagacatcc gtacgcaatt
     3841 gcttacccag tttctgtgat tcacgagtag caaaaaatgt atttgcgata gcagctaaga
     3901 tactgatcaa agaaaagccg atcatcacaa aacctgtatg ccaaatataa tcgacatctc
     3961 cttgcgctac ccctttatca ataatattgg aggtaagtgt cggcaaatat aaatctgcca
     4021 gtacttggat caccatgaaa actgcagctg caattgctga agtcaaggag atccgtttta
     4081 cgatttttag catctagttc ccttctttct atttatatac tccgtttttc agccaagaaa
     4141 gtttcaaatt gaattcactg attgcttgct ctactgagta atcttcagat gttgaaaata
     4201 agcgatacga atgcgcaatt gttgtaaata cagtattcat cactaattgg atcagtaatt
     4261 ccaattcatg atcatttttt actttcagct tcgtccgatc gatcaattca agcaactctt
     4321 ttgcatcttg tttgtgttct ttatgtggat cgtgaggcgt tttttggcga ctcgtatgag
     4381 gagcgacttt tcttgaggca cggtaatcca tgttcataaa caagttacga taaaaagaag
     4441 cattctcgcc ttccaaaatc tcataaatca ttttagaaaa ataggattcg aatcctttga
     4501 atagatcacc atcggattct ttcattagtt gtaacatatc tttagagctg tcacgtctca
     4561 ttgtctcaaa gtaataaaaa taaagatcct ctttatcttc aaaatactga tagaaactgc
     4621 ctctagggat atccgctaat ttgacgatat tagcaatcga agcttctttt aagggtactc
     4681 tagaaaactc gattctcgca gcctcgatta gtcttttttg tttttcttta ggcaaatgga
     4741 aaaatgtctg tgtcggcata tggattctcc tttctttcaa gatgacacat tgtcatatga
     4801 cgctatgtca ttataacacc tcctacctgt tttgcaagac atttgcatag tttttttcat
     4861 tatcatccga tacattaaaa aagtgataag aggaatcctt ccatttgaat taatgagagt
     4921 tgcccattaa aatagccaca tactaaacaa aggatgagat aaatggccca atcaattagt
     4981 gtccaagact tcaataaatt gcctctcgat gctgacagca tcgttttaga tacaagaaat
     5041 accttagatt acacaaaaaa ccacttaagc cactcgcttt ctatccccgc tgctatcttg
     5101 ccaagacaat taaaaaatct agatccaaag aaactttatt atattctaag ctatacaggc
     5161 aggcgatcag aagcattagc cgatcaactt actgctaatg gttttcgtgc tgtttatatc
     5221 attggtggga tggaagctct tcagtcgagt gttgcttgaa tattgcttgt tagattcaaa
     5281 agaggctgtg acaaaaaatc gtgtcacagc ctcttttata ccctgtcttt ttttagttat
     5341 ttattcttca accttatcaa tatgacttga tagccttttc tttttcgagt aaactgattt
     5401 tgttagtcat agttataaca atcatagcaa taaaaggaga aggtaagcat atggcatctg
     5461 ttttagtagt caaaggtcat cccctaactg cagaagaatc gcgcacagtt aaggcattga
     5521 cgtctttttt aacaagttat aaagaaaatc atccagatga tgaagtgact gttttggaac
     5581 tttatcgcga tgatattccg gaaatcgatg aagaattatt atcaggttgg gaagcattac
     5641 gtgctggtgc agaattcact tccttatcag aaagtcaaca acaaaaaatt gctcgtttca
     5701 atgaattgac tgatcagttc cttgctgctg ataaaatcgt tatcgcaaac gccttatgga
     5761 atctaaatat cccaacaaaa ttaaaagctt ggttcgatac cgtcaatgta gctggaaaaa
     5821 cgttccgcta tacagaaaac gggccagaag gattagtgac aggtaaaaaa gcactgcaca
     5881 tccaatctaa tggcggtgta tacaacggac aagactttgc ctctcaatat gtaaaaggta
     5941 ttttaaactt tgtaggcatc gatcaagtag atcagctatt catcgaagga atcgattatg
     6001 accctgaccg tgcagatgag ttgatgcaaa atgcattaga taaagctgct gctttaggta
     6061 aatcatttta aaaattgaga taaatgcata caaaagagac agaggaaaaa atcttcaaag
     6121 cagcatttgt gccttggata ttttcccatc tgtctctttt tgttattctt tctgcttttt
     6181 caacggattt tcttcataaa gagaagagat tgttgtgtac cattcaaaaa catgagtaat
     6241 cactacttta gctgctgcat aaacaggaat ccccaagatc acaccgatga ttccgaacaa
     6301 tttcccagaa gtcagcagaa ctaataaaat cgttactgga tggattgcta actgattccc
     6361 tagtactaat ggagagatca agcgtccttc tattgtctgc tctactgcaa aaacgatcag
     6421 tactttgatg atcatgaatg gacctgctac aatggctaaa aagatagccg gaatcatcgc
     6481 taaaaatgat cctaagtaag ggatcaggtt taaaaaaccc gcaataatcc ccaacgtgac
     6541 agcatactct aaaccaatga ccgcaaaacc aatcataaac atgattgcca cagcaaaagc
     6601 cacagttagt tgtcctctta tataagaaga aacttgacta ttcatttcac tcagcacatt
     6661 caacgtcggt ttttgcatgc gtgtcggtaa aaatttgaca aaataaggtg ctaattgttt
     6721 accgtctttc aatagataaa ataagataaa cggcatcgtc agcagagcca ctaaaatagt
     6781 cgctaccgcg ccgacaaaac ttccaagact ctgaactgta ctcttagata tatcttggat
     6841 catatcgcct gccgaattca tgaacttatc acctgctgtt tccaactgtt cgcgaaattg
     6901 gttgaacagc gggtcccgca aaatctcggt taatttatta tcaatcgtat ctacatattg
     6961 agggaagtta ctgatgaagc tcactgtttg ttcctgaatc ttagggatga tcactacgct
     7021 cccccatgcg attagtgcaa caaccaaaac aaacagccca atgatactgt aaagccttgg
     7081 tacttttcgc ttttcaaagt aatcaactac cggattcatc aagtaataca agattcctgc
     7141 taaaatgata ggcagaccta cgattgctaa aaattgccag attggtgcaa acaaatatga
     7201 aacttttgta aataaaaaga cgatcagcaa tattaacaat acaacaagta aggctgtgac
     7261 tacttgatta tttaaaaacc agcgccaaaa ccacgatacc ggtctgttct tcttattttt
     7321 tccttccata attcctcctg ttagttataa aaaatttggc ttcccaaacc aatagcaggc
     7381 agtctatgcg gatgaagata gattccattt gcaatttgtc cttcttctgg acaagcatca
     7441 aaaatcaatg atacgtgagc tactgtcttt aaattcccat tggccatcgg accgacataa
     7501 tcgatcgtat atacctgatc atcaaaagca attgttccgc ctgctttcaa agaaaaaggt
     7561 tcgtcttttg atacttcttg aatcaccgaa tattttcgta aggcagctgt tgctgtttta
     7621 tcgaacaaaa tcagcatggg ttcttccttg tttattgctt ctgtcccaat ttctgtaatc
     7681 tttgcctgaa tcattgcact ctctccttta attgctttca aaaacagtat aacacattct
     7741 cattctttag ccgtgtggaa taattatgtg atagaataaa aacgaaaact atttcattac
     7801 aattaggagg aatcagctca tatgatcgaa aagataattt taggaaccta tacgcgtcgc
     7861 gaaagtaaag gaatctatac gatcgacctt gatacagaaa aagaagaatt agccaacctt
     7921 tcattagctg ttgaagaaaa tagccctacc tatgtagcaa aaagtaaagc tggcaatctt
     7981 tacacagtta ctagtgtgga tggattaggt ggtgccggtg cgtacgataa ggattttcat
     8041 tttttgaatg cggtaacaga gtctggcgct cctttgtgtt atgtagcggt tgatgaagca
     8101 cgtcaattac tatacggggc gaactatcat aaaggtgaag taaatgtcta ccatatatta
     8161 ggagatggcg aactgaaagc agcagattca atattccatc aagaagcgac aggtcctcat
     8221 aaaaaccaag atcatgcgca tgtccactat acggatctga ctcctgacca acgattagtc
     8281 gtatgtgatc ttggtacaga tcgtgtgtat acttatgatg tgtccgaaaa cggcaaattg
     8341 aatcttgtaa ctaccttcac tgcagagcca ggaacaggcc ctcgccatct tgtctttcat
     8401 ccaaatggac aattcgctta tttatttggt gaattagaca gcacagtccg cgtgctttct
     8461 tataataaag aaacaggtgc ttttgaagaa ttacagaaaa tatcgacctt gccagaagaa
     8521 ttcgacggtg aaaatggagg ggcagctatc cgcatttcaa atgatggcaa gttcctttat
     8581 gcttctaacc gaggacataa ctcgattgct gtattcgcag tttttgaaga tggttcccac
     8641 attcaaaaca agcaaatcat ctcaacagaa ggggattttc cccgcgattt cgcactagat
     8701 ccaagcaata attttgtcgt gtgtgccaat cagaacaccg ataatctgac attatatcgc
     8761 cgtcatcctg aaacaggatt actggaatta ctgaaaaaag atgtctatgt accagaatgt
     8821 gtctgtgttt attttgatcg ttagtaagaa aaggagaatg aaatgaatca gcaagacgta
     8881 ttagacagac tacgggaaga actaaatatt cctttctttg atgggaaaat cgaagataaa
     8941 gagtatagcg aagaggaata tcagaaactc aaagccgatt tacagaacta ctttgaacaa
     9001 tatgtacaga atgtagaaaa ctaatcagct ttccatacta tctgaaaaga acatcaggac
     9061 aagacttttc cgtctatcct gatgttcttt tctacaaata aaaaaccgcc gagatggtta
     9121 gactcgacgg aaaaggagta tttactcgat tttgagtaag tacaaggttg ttgttggtta
     9181 taggtatgtc attatcataa aggattttga tcactctatc ataaaggtaa tattacattt
     9241 atattagata actctagttt ttattgttat caaagatttc ttttattttt tcaacaacaa
     9301 attagtcatt ttttggatgg cagctgtcaa ttggtctgtc gcttggtctt gttttgcttc
     9361 agatgtattg aatagatctt catctacccc tgattcaaga tcgatacttt ggatcatttc
     9421 ttcacagcct gctacatatg aaagataagc tttttcaaaa tttttatgaa tgcccattgc
     9481 ttttgctggt ggacgcaaag tactgacttt cataagcatt gcccgatatt tttttgttcc
     9541 ttcttcaaat aacaggatga tctcttcaag agtcgatttg tccagttcac ttgtttttcc
     9601 ttcatcaatt gcgtttctga tcacttcata tttcggatgc attccttctc cgattgcttc
     9661 tgtttccttg acgatttgat cgatggactg cacataatag ccaatattcg gtaacatgtt
     9721 cgttttccta ctttctccat tgttgttatt atttattcta cttgttttta tcctcaaatg
     9781 acaagtactt gaaaaaattt aatggtttct atacagctcg ccattcttat tcgtgctcaa
     9841 agaaaccatc tgctaaacta tattgtttcc ctgcgacaat gtcatattgg atcaattgat
     9901 attcgtcgta aatctcttgt tgcgagcgat tgaattgcgc ttctttatac cactgtccgt
     9961 tttgatagta cagttctcgt tcaaacgccg gaagttcctg ttctaatgat aaaagcagct
    10021 gataaaagcc atttgttgtc tgattcgtct gttccattag attcgctgca aaataaaacg
    10081 gactggtgat tgcatcgtga gtcgtttgct tttctaattc tcctttactg tcgaacataa
    10141 gaaattgtgt ttcatgcaat gtttgtttgt cattttttgc ttggatcgta tctgaataga
    10201 tccctggaag atgatccccc caaaaaacaa ctaaagtcct tctagaaaga ttcttcaatt
    10261 cttccgtgaa ctgcttcagt gccgtgctgc tataggaaat atcttgcaga taattctcta
    10321 agtcaagtgt cccacttcca tctgaagttt ttgcaggata gtctagatta tcatactttc
    10381 catcgtaggg catatgtgtc tgcatcgtga ctaaatgaat gaactgaggt gttttttctt
    10441 ccttgagaag tgtcattact tctttgtaag ctgactcatc agaaatatat ggattattct
    10501 ctatcgtatc cgtataggtc atcgtacgct cgctgatgaa ctggtcaaat cctaatgtct
    10561 gataaacatc ctctctttta tacatcgaag tattgtatgg atggatcgct gtcgtctggt
    10621 atgattgtgc gtttaacgct gaaacgatag aaggaagctg atccattttt ggtacaagca
    10681 tcgtgtaagg ggtggtgagt tgcgcattaa ataacgccat agaaaaacca gtcaatgctt
    10741 caaattcgat atttgctgtt ccaccgccat agttttgaga aagcatattg ccactatatg
    10801 tttgatcagc tacttcataa taatctgcca atggctcacc agatacttct acgccgttta
    10861 atcgactagg atctgagaaa ctttcactca tcacgaacac gatgttcggc gactcttcct
    10921 ctacagtttt atttttttca tcggctagct tctggtattt ttcagtgatt tctttgatct
    10981 tttctttcga atatccttca ggttcatcca ttggttctac cttcagattg tacaaaaacc
    11041 caccgataaa accagtgtta tagtagttca ttttctggct gtaaggtatc catagtgccg
    11101 ttttattata tgcttttcgc aacaaattat cgggattgtt gaaattactg atatacccta
    11161 gcagtccaat tgccacaagc agtgtcgtta atcggatcaa ttggaatcgc cttccttttt
    11221 tcaggcttcg aaacatgtac cagcaaaaca atcctatcac ggttcctgcg gcagctaaga
    11281 tcactgtaaa cagcatagtg ccggtcatat ctttcagcaa tccgatttca gtgatcatct
    11341 tcaaatcgtc tggataaatc ggttcttgcc gataactcat tttcatataa tttgcaaaac
    11401 ctaatacacc gatggctacg acatacgtaa gggaacctaa agggacagat cctgctagtg
    11461 caatcagaaa aataagaata atcagcaaga cgagacaggc taagaagaat ttttccgtat
    11521 gccaagaaaa agcaaatttc aacgcaaggt ctacggatag atcattttga caccactgca
    11581 aaaataaatt actaaaaaca acaagaaaaa agatactgat gattgctgct gttcctttta
    11641 aaatcttttt ctgtttggtc gaaagtctgc ttatatatct tttcaaatgc ttgctccttc
    11701 atttctatca ataaaagaat gttgcttctc ttattttagt tgtacgaaat tcgaaagtaa
    11761 agaacggaaa gaagaattaa tgaaatccaa atattctctt tctttatggc ctttctggtt
    11821 tctttaataa gcggaccttt cgtactccag aaattttttc tagctgttgt ttcacttgat
    11881 tgagctgttc aaaatcagta ctgtctatgt caatcaatat gtagacatgc tcattcttat
    11941 ggttacgcga tatatctgcg atcgctagct cgttctcatt cagcagagaa aatatctctg
    12001 ctaatatatt ggttttcttt tgataaaata tagtgaatcg aaacggcgct tggaaaaaca
    12061 ttcgtgcgga tggataattg accgcttctc taaccgtacc gaacaaaaga taatctttta
    12121 aggcttcaac tgccaaacgt ccgctgtctg ccaaagcttc ctgagtcgat ccccctagat
    12181 gaggcaacat aagtatacgg tcattttcta agaactcttc ttctggaaaa tcagtcaagt
    12241 atttagccag atgttgtttt tccaatgctt gcttgcataa cagcatattt atcaacaatt
    12301 tcacttcttc ctacattgat aagtacagca tcttttttca ttttttctat attcgcctga
    12361 tcgatcaatc cttttgtatc ttctgtcaac ggcagcatga ctacgatgta atccgaggta
    12421 gaaagaactg tttctaaatc tgcttgccga acataatcca actgggcatc gcggatagaa
    12481 taacccaaga catccatccc taagctatag caacttagcg ctacttttgt gcctatcgct
    12541 ccaagaccaa gaagaccgat ggttttgcct tgcaattctc gtcccacata agcacttctt
    12601 ttgttttctg cctgttctaa aatattagga cctgttagtg tttgtaccat acgacttgct
    12661 tcgattatcg gacgggaaga taagagcaaa cagcacaaaa caagctcttt tactgcatta
    12721 gcatttacac ctggtgtatt catgacgatc gtcccgtttt cagaagcttt ttcaacgttg
    12781 atcgtgttca ccccaacacc agctcttgaa attgctagca aatctggtgt aagccattca
    12841 tctttcactt tcgtactacg aataactatt ccttcggctg gcaaatcatc tattattttg
    12901 aaatattccc cttgtaactt ttctatctct tgttctggaa tagcatcaat caaatgaacg
    12961 tgatacataa aaccccctcc ttactaacaa gtatctcgaa atttctgcaa ttagacaact
    13021 gtttaccatt tgacttccta gaagcaaaaa gagcagttag agcagagtag gctgagtgat
    13081 aaaaataagg ctatgacaag ttactgccat aagccttatt ttcgagagca aaatattgag
    13141 ggtgagatat cttgataaat ggtgttccgt tagctgacat tgataataag actaaatttc
    13201 aaaaaagttg aaaagcaaat ttcgtcaatc tcttcttatt tcttaaaact aagtggcttt
    13261 ttcacaacct ctttttatct aataaccact gtttccaact caactaattt tgcccacgtt
    13321 tcaatttgat ctgttgttag atttagcgag acaacggtat gatgaccgcc acctgcctga
    13381 atccatgaat ggacaccttc atggaaattc ggtttagttt tccataacac acgtgcaaca
    13441 ggtaaattag gtgcttcagt cgttggttca aaggcttcta cttcattgat cagcagttta
    13501 taatgcgtac cgaaatctgc catcgatacg actacccctt ctcctgcttt cccatcaaat
    13561 acaagacgcg ccggatcttc tctatctccc attgataaag gagaaacaac gattcttggt
    13621 ttcgtcgcag caagcgtcgg atctacttcc atcatatggg attccaagat tgcttcttgg
    13681 ccactagcta gctcgtaagt ataatcttcc atgaatcctg tatctttccc tcgagccatg
    13741 attttcagca accgatcaat cgctgcagtt ttccagtcgc cttccccagc aaaaccgtat
    13801 ccttctgcca tcaaacgttg tacagctagt ccaggtagtt gtttcattcc atacaggtct
    13861 tcaaaattag tcgtaaaagc gttatagcct ccttcttcta agaaacggcg aataccgatt
    13921 tcctgctgag cttgtacttt tacatgttcc tcccatgtct tttcttcata atcaccatag
    13981 tcgaaggtat acaattcctt gtactcttca aataattgat cgacttcttc gtctgatacg
    14041 cgatcgatta cttgtacaag atctccgata ccaaaataat caacagtcca accaaattga
    14101 atctgtgctt ccactttgtc tccttctgtg acacccacgt ttctcatgtt gtcaccaaaa
    14161 cgagccactt ttattccaaa actttcatta taggctactg caacatccat ccaatcagca
    14221 atctcttttt ggatctcagg tcgtttccag tagccaacga cgattttatt ttgtttattt
    14281 aatcgagcat tgatgaaccc atattcgcgg tcaccatgag cggattgatt taaattcatg
    14341 aaatccatat cgatcgtttt ccaaggaatg ctttcgttat attgcgtagc taaatgaagc
    14401 aatggttttt gtagaagctt tgtcccgcgg atccacattt ttgctggtga gaaagtatgc
    14461 atccaagtga tcacacctgc tacttcttct cgataattga cctctttcat gatttttgtg
    14521 atcgtatcag cagtaactgc taattcttga aggcggattg gataaggcaa ttggccattt
    14581 tcattcagtt catcgaccat ttcttgtgca tgtttcttta cttctcgcag tgcttcttcc
    14641 ccatataaat gctgtgatcc tacaacaaac caaaattctt tttctccaat attcaacata
    14701 attagccact ccttttattt gttttgtccg taatacgcat tttttccgtg ttttcgtaaa
    14761 taatgtttat ctaaaatccg ttgaggcagc tcttctgaaa atgaattgag ctgtctggta
    14821 aataaattca tcttgcatac ttcatctagt acgactgcgt tcattaccgc actttgcgca
    14881 tctttccccc aagtaaatgg accgtgtccg tgtaaaagta cccctggaat tgctaaggga
    14941 tcaattttgc gttctttgaa tgtttctatg ataacttttc ctgtttcata ttcgtacccg
    15001 ctgtcgattt cctgttgtgt caaaaatcga gcacaaggta ccgatccata aaaagtatct
    15061 gcatgcgtcg tccccatcgc aggaacatcc aatcctgctt gtgcccagat cgtcgcccat
    15121 gtcgagtgag tatggacgat tcctccgatt tgaggaaatg aacgatataa aacagcatga
    15181 gtaggcgtat ccgaagaggg attgagttta ccttcaatga tatgattgtc caagtccaca
    15241 acgaccatgt cgtctgctgt catctcttca taactgactc cgctaggttt gataacgaaa
    15301 tatcgtgttt caggatcaaa agcgctcaca tttccccatg tatatttgac gagtccgtgt
    15361 ttaggcaagt caagatttgc ttggtatact tcttctttca gttgttccag cattttctat
    15421 cactcctttt tcttgcaatt gatgaatcgc tgttttttca atgtcgatcc cctcacggta
    15481 acgtctcata aatatctgat agcctgctat ttcttctgct gaaggtgtga tggtgattcc
    15541 ttggtcttgt ccaaacacgt cttctgccaa gaagttttct aaagacttcc catgtcgatc
    15601 catcatatag gctgctaata acgcgattcc ccacgctcca ccttctccgg ctgtgtccat
    15661 cactgtaact ggtgcctcca tagcagatgc tagaatccgc tgtccaactt ctggtgtttt
    15721 aaaaatcccc ccatgcccca ctaaacgatc aatttggaca ttctcagatt tcaatatatc
    15781 cattccgatt ctcattgcac caaaagcact ggctaaatgg atccgcatga aattcgctaa
    15841 atcaaaacgg ctacctggtg tacgtacaaa taatggcctt ccttcattta ctcctgtaat
    15901 gttttcaccg gaaaaataac cataagaaag taatcccccg caatccgtat cgccttctag
    15961 cgctttgttg aataataccg tgaataattt atctctgctt atctccatac ccaaagaatt
    16021 ggtaaattct tcaaaaatac tgacccaagc attgatatcc gaagagcagt tattcgtatg
    16081 aaccatgcct actagttctc ctgaaggtgt agtgaccatg tcgatttccg gatggacatt
    16141 ttctagttct ttttctaata cgatcatagc gaaggcggaa gtaccagccg aaatgttccc
    16201 tgtccctttg gctacactgt tcgtagcgac cataccggtt cctgcatccc cttctggcgg
    16261 acaaaccggg attcctggtc tcaaatttcc cgtagggtcg atcagataag cccctgattc
    16321 gcttaacttg ccagcttctt ctcctgctac tagaacttta ggcaaaatgt gatccaagtc
    16381 aatcgctacg tgcgcttttt cgattaactg accaaaaata tttttcattt cttgatggta
    16441 ggttttcgtt gaaggatcga taggaaacat gcctgatgca tctcctacgc ctagtacttt
    16501 ctcaccagta agctgccaat ggacataacc tgcaagcgtt gtcaaaaaat taatttcttt
    16561 cacatgttct tctttattca aaatagattg atacaaatga gcgatactcc atctctgcgg
    16621 aatattgtat tgaaattttt ccgttaatat tcgttctgct tcttcagtga ttgtatttcg
    16681 ccaagtacga aatggtacta gtaactgttc cttgctatca aatgctagat aaccgtgcat
    16741 cattgcactg aacccaatcg acccgattgt cgtaagattc tcgccataga tttcgaatac
    16801 ttctgctgcc atttttcggt aactgacgcg cagtcctttc caaatatctt ctaatgaata
    16861 ggtccagata ccattttcta gtttgttttc ccactcataa ctaccagcgg caataggagt
    16921 aaaagatgaa tcaatcaata cagctttgat cctagttgat ccaaactcaa tccctaaaga
    16981 tgtacgacca tctatgacat ctaacatact ttgcgcattt acttccttca taatgattta
    17041 actccctcct tctcgtcaaa cctgatcaaa ccgtcttttt agtagcgctt acaatgatta
    17101 ttgtatactt ttgtacgtac atgtcaagca gttatttaca agaatacaat aaaaaacact
    17161 tgttttgtac gtacataagt tataaaatga aattgcttac aaaatctgat caaccgcctt
    17221 ctcgaaattc tagaaggaga gaatacttat gaaaaaagaa aaaaaagttt cctcatcgtt
    17281 tatttatttt tttggcgcat ttggcggcat tctatttgga tatgatattg gtgttatgac
    17341 cggcgcctta ccttttttac agcatgattg gggtttggca ggtaaagctt ctttgatagg
    17401 atggatcacg tcttcggtca tgttaggtgc gatccttggc ggctctttat ctggtcttct
    17461 ttctgataag cttggacgac gaaaaatgat tcttctttct gcacttattt ttatggctgg
    17521 ttctgtcctt tctgcatctg cccctcacaa tggttcttat tttctgattg cagcaaggat
    17581 tcttctaggt ttagctgttg gcgctgcatc tgccctagtt cctgcttata tgtctgaaat
    17641 ggctcctgct agactaaggg gaagattgtc agggatcaat caagtgatga ttgcttctgg
    17701 catgctcctt tcctatgttg cagattattt gctaaaaggg ttgcctgaaa cgatggcttg
    17761 gcgcgtgatg ttagggctcg ctgctgtccc cgcgctgatt ttatttttcg gtgttttagc
    17821 tctaccagag tcaccgcgtt ttcttatgca atctggccgc ttggaagagg caaaaagagt
    17881 attgaactat attcggacac caaaagaagc tgaacaggaa ttcgaacaaa tccagctgaa
    17941 tgtcaaacaa gaaaagacaa ccggaacttc ttggcatact ttgtttttag aaaaatatcg
    18001 ttccttagtc attgccggta ttggtgtagc agtattccag caatttcaag gagctaatgc
    18061 tattttttac tatattccgc tgatcgtaga aaaagcgact gggcatgcag cgagcgatgc
    18121 attgatgtgg ccgatcatcc aaggaatcat tttagttgct ggttctttgc tctttttggt
    18181 tattgcagac aagttcaatc ggcgaacttt attgaaaata ggcggtagcg tgatgggact
    18241 ttctttcatc ctaccagctg ttttaggaac tgtactggat gctcatacaa attcgttgtt
    18301 gattctcttg tttttatgta tttatgtcgc attttattct tgcacatggg ctccactgac
    18361 atgggtaatc gtaggcgaaa tattcccact agctgtccgc gggcgagcat ctggcctagc
    18421 ctcttctttt aattggattg gttctttcct agttggcttg ctttttcctg tgatgactgc
    18481 ttctatgtct caagaaatcg ttttcggcat ttttgggatc atctgctttt taggggtttt
    18541 atttattcaa gagatcgtac cagaaactcg tgggaaatct ttagaggaaa tcgaacaaag
    18601 tgcatctaaa aaaacttatc ctaaacgtat ttctatccat cattcttaaa gaggttgtga
    18661 cagaagtggt cagcttcaag aaataagaag gaaccgctta gtttccagtc acaaggaaca
    18721 atttcaaaaa acagcttctc atgtttgatg aatattgtga cttgtgacca gttggtagga
    18781 gaacaccgtt tagtggagag gtggtgaaag aaacgttcag ccttgagcga tgacaaaagc
    18841 tctcacaaac attgcgacga gtaaccgtag gagcaataag aggcggtaaa aacccgtctc
    18901 ttatttttcg ttttctttca ctgtttcccg ttcaactagt acaggttcat agagaatatt
    18961 tggctgtgcc tgtccttttt caatcgttgt gacgatccac cgtgcggcat cttgtcccaa
    19021 cttttctttt ggatgcgtca atgttgtcaa ttttttattc cctaaatggc ttaaagaaga
    19081 atcgtctgtt cctacgatcg ataggtcatc tggaatatga tagccatttt ctgtcaactt
    19141 atctagcatt aaactagcta tttgatcgtt atagcaaatg atgccatcta aagcttgttc
    19201 ttctttcaac cgttgtactg ttttttcagc gattgtttct cgtgtttccg tggtgaaagt
    19261 aatgatatct tccgtcaaaa aacgcacatt atactgttca cacgcgctga taaaaccttt
    19321 catcctgtat tttccttgaa gatcgtcgat ttttgtgatg aacaacagac gctgatgtcc
    19381 tttttttatc aaatattctg ctgccataaa accgactttc acatcatcta aacaaatata
    19441 agaggcattt aactcttcat aagcagcatt gatcatcacc atcggaatgc cttgttctct
    19501 caaagcaaca taaagtgcta agtttgggtt atactgatta cttttcgtag gctcaacgat
    19561 caaaccatct accccataag caatcatttt cttcaaacac tctttctcca attcgtgatc
    19621 gttattcgta ctggctaaaa gcaatgaata cccagattct cgtaattcat tttcgattcc
    19681 tcggataatc gacggaaaaa tataatcaga gagatatgtg gtgatgacac cgatcgtttt
    19741 tgttctgttc aatggtgttt tttctttctt atagctgtca tctacatatg tgcccgaccc
    19801 tttttcttta cgcagatagc cctcatttac caacacagcg attgcttgcc gaatcgtatg
    19861 tctgctgacg tcatatattt cttgcagctt gaattctgag ggaatggttg tatttacttc
    19921 gtattcctca cgaaggatct tttgttttat ctcttctgca atatcttgat attttgccat
    19981 ttgccttgcc cctttttatt tgtacgtaca tatatcataa cgagtctgca aggtttattc
    20041 aagcaaaaat caaggtttct tgtgctcata aggcatacgg aacaggttca ccaaattctg
    20101 gaaatccttg ctcattccac gtaaagcatt gcgcgttcgc gtgccgatta ggattgtcta
    20161 acggatcccc tttttggttc ttctctggtc gagcatgata gatcaaaata tcaaagcacc
    20221 cgtcttctga aaccgtaaac gagttgtgac caggaccaaa ttgcttattt tttttagaag
    20281 aaacgaatac tggctccgat aatttatgcc atgattcttt taataaaaga ttcttgtgct
    20341 catcagccca cagcaggccc atggcatagt tttcatctgt tgcactacca gaatacgtaa
    20401 tgaatatttt cccattgcga gtaaggactg ctggaccttc atttactgaa aaaccccgtt
    20461 tttcccattc atactcaggt atagaaagga ggatctgctt cccctttaac gtccaaggat
    20521 tatccatttc ggatagataa agattagaat tcccagtaat ttcaggatct tgttgggccc
    20581 acacataata tagctttcct tgagaagaga aagttgttcc gtctaaactg aaagtttccc
    20641 ttttcgtttt tatttgtccc ttttctatcc aatctgtatt cattggatct atatgctgat
    20701 tttcaagtac gaacatgcgg tgatgatgag attcatcacg tatagtttta tgattgcttg
    20761 cagcaaagta aatataccat tttccattga tataatggac ttcaggtgcc cagatcaatt
    20821 cactcatcgg tccttcttca tgagcctgcc atacaatcag gcttgcagca tgttctaatt
    20881 cattcagtga ctttgccctt cttaattcaa tcacttggta gcctggaacc gatcctgtaa
    20941 aataataata accatctgta tgtttatata cccaaggatc tgcccgttgc cgaacgatag
    21001 gattattata aagtgtcttc atcataacaa atttcctcac attttcttta ttctaattac
    21061 attccaagag tatttttcta tccctacatt taattcgtca tgtttttcta ttgaaaaata
    21121 gtttatttca ggttttattt tttctggttc ttcacgtgta tttttgtcca acagatcgct
    21181 actggttaat gtgtagtgct ctccttgatc tagcaaattg aaatcatgtt caatcgttat
    21241 cttttgttgt tcatttgtgc gattgatcaa gaaaatagtc agctgattgc cgttatctac
    21301 gatcacactg tccacataag gaacattttt tatcttttca caatcataaa cagggcaaat
    21361 gatttgttgt ttcaatactt gacctttagc atatttcgat aagtgttgca tcacataata
    21421 agttggattc ctccatgctt gtcctttagc atctgtcagt attaaaggta ttgtgtttac
    21481 taacaatgat tgacaactaa tctttacgcg atctgcgtgt cgtaaaatag ccagccctaa
    21541 tgaaccaaat aataatgtat cttccatagt aaagtaacac caatcgatgg gactaccgac
    21601 ttcaaaatcc ctatgtcttt tttccggttg ggcatgtaca ttccattcat caaacgctag
    21661 atacattgtt ttctcacttc tcaaatatcc ttttacataa tcacaagtag caacgatttc
    21721 ttcaatttga cgatcgaatc gataagcttt tgcaaggtat gtcgctgtat catcacgctc
    21781 atttttagga ggtttagtca agtcttcttc atcatactta tcaatatagt tatgcaaagc
    21841 taaataatct acattctcat aagcttcttc taatgcgata cgatcccatt cagggtacga
    21901 tggtaaacgt ggcgtagagc ttccaactaa gattgtttca attgaatcat ccactaactt
    21961 catgactttt gaggcttcat ttgctaacct accatattca taagcactct tagcccccac
    22021 ttgccatgga ccgtctagct cattccctaa acaccataat ttaacgccaa aaggcttttt
    22081 ttgcccattt tttattctta aatcactcca gaaggttcct tttggaaaat tacaatattc
    22141 tactaaattt tttgcttcat caattccttt tgttcctaaa ttcaccgtga atatcggttt
    22201 cgcttgtaca tgactgatcc atttaaaaaa ttcgtggagt ccaaaagaat ttggttctat
    22261 acctcgccat gctaaatcaa ttttttttgg tcgttgttct ttcctaccta cagtatccac
    22321 ccaatcataa ccagatgtaa agtttcctcc aggatagcga atgacaccta attctaattc
    22381 ctttacataa gagaggacat cttgtctaaa cccgtcacca tcagctgttt catggcccgg
    22441 ttcataaatc cctgtataga cgacacttcc catatgctca acaaatgagc caaacagccg
    22501 agaactgatc tctcctaccg taaaatcctc tctgattttt actttcactt cttcgtccaa
    22561 cagctttccc tacctttcat ttcctatagt caagataata gatacaagtt tccatacgcc
    22621 accagcaaag ataaaaataa aaggagggaa aagaaatatg agcagtacaa atcctaataa
    22681 caatacgcaa caaagaatat ttctcaaaaa atcaaaaaat actttccatg cactttgttc
    22741 aaaaatttgt aaaaatgtga tatctttgtt ttcagaaatc acatatatcc aggataaaag
    22801 aaacatggaa taaataagta aaactacata tccactaatt gaaagtacga ttgtctgttc
    22861 ttctaataat tgattaacaa gaaaataaat aatgatcgtt aacagactaa ggaacaagtt
    22921 cttaccaaaa tgttgcttta ttccttgaaa gaaaaaataa atgatatttc ctgatccctc
    22981 acctttccat tgcgccatta cataaaaaac agcacatgta gcaggcacga tagtaataac
    23041 aggcaaacac acgattaccc agagaatacc taataagatg taatcagaaa ccacaatcaa
    23101 ctgtccataa agtttagtct caactatttt catcttcctc actctttcat tccagacata
    23161 gctgttcctt gaacaaaata tttctgtgca aaaatataaa ccactagtaa tggcagcatt
    23221 gaaataaccg ttccagccat tacaggtcca taaaaagaaa tgttctgccc tgtaaataaa
    23281 gctaaccctg ctggtaaagt tctcattttt gaactacttg tcaatatcaa tggataaagc
    23341 aagtcattcc aactattcat cagcgtgaaa atccctaatg ataaaagagt aggtttgctc
    23401 aatggcaaca tgattttcaa aaaaatatta aactcactta atccatcaat gcgtgctgcc
    23461 tcttccaaag aatctggtaa agtaataaaa aaggaacgca tcatgaagat accaaatgca
    23521 gttgtcatac gaggaatgat caaacctgca taagtatcaa gcatgccaag caaattgact
    23581 tcaataaata gaggaatcat gaagacttgg aatgggatca tcattgtcag caaaacgaaa
    23641 tagaacaaaa ctgattttcc tttgaaacgc atacgagcaa atgcataccc cgcaagagaa
    23701 tcaaagaata cagaagtgat cactgtccct ccagcaaaaa tcactgtatt tttgatgtaa
    23761 tctaataaag ggatcgttgt ccaaacctta atatagttat ctagggtata gctatctgca
    23821 aatattctag gcggaaatga gataatgtct ttttcgtgtt taaaagaaga aataatcagc
    23881 cacaaaaaag gaaacgcaat aaccaacaat ataactagca tactaatcgt tcgaatgatt
    23941 tttttaccac taactgcttt tcgactattc acagtaactg taatcgagct ttctggtgcc
    24001 atacaggttt ccctcctatt tcatttgttt ttctttccgt gacatgtaca cattagtgga
    24061 gatagctata gttgcaacaa tgataaacaa aaccgaagac agtgctgatg catagcccaa
    24121 ttcataaggt gctgtaaatc ctcgactgta aatatattga acagctgttt ctgtcttaaa
    24181 ttgtgggcca ccagcggttg caacatatac ttggtcaaac acttgcatcg aaccaatcag
    24241 attcgttaaa atacaaaaac ctaaggaggg aacgatctga ggtagcgtaa tgttaaaaaa
    24301 ctgctgtcga tttgttgcac catccatttg agcagattca tacaaagagg gagagattcc
    24361 ctgaatagca gtcaagagaa tcgtcatagt aacaccaaaa ttcttccaaa cagtcatgat
    24421 ggctaccgtc ggcatagcaa gggttggatc gcgaaaaaat tgagggatcg gcaagccgac
    24481 ttgtgaaaac aagtaaggaa tcgcccctac attcggatca agcaacatag aaaaaataat
    24541 cgcaattgac gtcaatgaac aaataactgg aatgtaaaat gtcgaacgac aaaaacgatt
    24601 gaatctggta tttttagata atgctactgc tgctaataaa ccaacgataa tttgtgttgg
    24661 cgtttcgagt acggtgaaat ataaagaatg aatcaacgaa ttaactgctc gttcatcact
    24721 aaatatacgt gtgaaatttt caaaacttac aaaatttctt gatgtaaaga aaatattcaa
    24781 atctgtaaaa ctaatagcaa atgttcctaa aagcggtatg acagtaaata ataataaaac
    24841 taaaaaagat ggagttaaaa acaaatacgc agcacgatca ggacgattcc aatatttggt
    24901 gaatccgttc atatagtcca cctcatcttt cattttgata aaatatacaa tcttggttta
    24961 aacgaatctg aataagatat ccgaacaatc gctgtgattt ttgcaataac acctatctgc
    25021 aaatactagg tcgatcattc agatatctgt atcttgccaa cagtctatcc tgtataaagt
    25081 tctgtactat ttttgcagta attgattgat ggcatcatct gcatcgttca tgagtgtctc
    25141 aggatcttct cctgctaata atttttcaat cagtggattg atgatatcgt tattgatcgc
    25201 agagatttgg gtaaaacctg gtaaatatgg ctctgcatag tccatttgtt tcgataattc
    25261 agatacaata ggatcatttt ttacttcttc atcttttgct actgaatcca agtaggcagg
    25321 aaaaccattt tcgatactcc attttttacc aatttcagtt gtattccaat atttcaaaaa
    25381 ctcatagaca gcttcttttt tattattatc tgtacttgcc ggaatggcat atcctacgcc
    25441 atctaaaata gctgtttttt tgtctgaatc actcccttgt ggtaaggtag tcacattata
    25501 atcaatctcg tttgctttca atccgttatt taaccatggc ccattgattt caatggctag
    25561 ttgaccagca ttcattaaat tgtccatttc ggcgccagaa atcgattctg gaccagtctt
    25621 gtctacatgg atcatttttt ggatcgcttt taacgtttta aggttctcag gagacgcaaa
    25681 ttcagactta gtataatcat cgttcactaa tttaccgcca tttgctaaca tccagttata
    25741 taacggtgca gttccatcct tattgaagac aaaacctgat acatttttac tactatcagt
    25801 taattttggt gccatttctg tcaattcttc ccaagtagta ggtggttgtt cagtatctaa
    25861 tccagcttgt ttaaacaaat cattattcca atacatatac atgccttgta cctgcatggg
    25921 gacaaaatac tgtttacctt ctacttcacc taattcaata gccgtttctt taaaatcaga
    25981 tttatctact ccatcatatt cccagaaatc gtctaatggt tgtaccgctc cgttttctac
    26041 atactgtgca aaatctggat aatttaatgc aataaagtca ggtgcttttt tagaagaaat
    26101 cgctgtagga agtttttcat tcaaattggc ccaagtcatg atatccattt caattttgat
    26161 gtttttatca tttgttttat taaattcatt aacgatttct tgcaatattt caccatcaga
    26221 tgcagtaaac ccattccaaa aagtcaaagt cacttcttta tcagaatcag attctgctga
    26281 acttccgcca ccgcaagctc ctaaaatacc tgttgcagct accaacaaag agccaattaa
    26341 ccatttttta cctttcattt tttgttcctc cttcatctga taaacgacat ctgaccacat
    26401 tccaagatag cggtttcaat aaacaattga ttttcccatc ttcagctttt acatttgttg
    26461 aatgacttgg ttttacttgt tcacttttag gaagattagt tgcttttgta tcaaatccac
    26521 tcatttcaga aaaatcaatc actccctcta atgataattc agacaagtga acagttagat
    26581 tcatttcttc ttctccacgg ttaactgcaa atatgacaag ttctttgtca tttattacgg
    26641 ctatactttc taaatacggc acttcttcaa aatcttccgt cgcatagttt gctgaacgaa
    26701 tttgtgggct taacacctct ccttgtccat acacagcagc ttgcataaat ggataataga
    26761 tcgtctgtct ccaagcttct ccgtttgttt ctgtcctgat tggtgcaata acatttacca
    26821 attgagcaag acaagcaatc tttactctat ctgcatgttt caataaagtg attaatagac
    26881 agcctaatag taatgcatct tcaaaattat agttatcttc tagtaaagga ggagcaatct
    26941 gccaaggcgc tgcttgctca tcttgttccg ctgaatgata ccaaatattc cattcatcaa
    27001 aggacaggtt gatctgcttc ttactatgtt tcttcgcctt gactgtatcg cacatcgctg
    27061 tcacttcaga aatgaaccga tccatgtcta aggaacgtgc aagataatga gaaagatcat
    27121 ttttttggtt gccataatat tgatgcagag acaaataatc tatttgatcg tacgcttgat
    27181 caagaactgt taactcccaa tcaccaaatg taggcatttg ccgattcgaa cttccgcata
    27241 aaaccacctc aattgaatca tctacgagtt tcatcgcctt agccgtttcc tctgctaacc
    27301 gtccatattc ttccgctgtt ttatgtccaa tctgccaagg accgtccatt tcattcccta
    27361 aacaccatgt cttaattcca aaagggctct cttgaccatt cgaacgcctt aaatcactcc
    27421 aataagttcc tttaggaaaa ttacaatatt cgactaaatt acgagctgca tctactccac
    27481 gagtacccag attgacagcc atattgattt cggcccccac atgctgacac cagttataaa
    27541 attcatgaat cccaacttca ttcgtttcaa ttgtccgcca agctaaatcc aaccttctag
    27601 gtctatttgc tatgggccca atactgtctt cccaacgaaa tcctgacaca aaatttccac
    27661 caggatatct aatcatggga atgttcaatt cttttaccaa gtgaatgacg tcttggcgaa
    27721 atccactttt attagctgat ggatgatctg gctgataaat cccttcatat actgctcgtc
    27781 ccatatgttc aataaatgaa ctgtacaacc gcggatcaac tttgcctaca cgcttgacac
    27841 tactaatcaa cattgtggct tccataactg acctccttta atttatacat gaataataca
    27901 cctcgttgaa agcgttgtca ataatataaa tgttaaactt acttattttt tatcgatttt
    27961 aattaaccaa aatattaata atgattaaaa caaataattt aatataactt ttattaacaa
    28021 atatgttaat atataagaaa aaccttttaa gaggtgatat atatgcaact acaaataaac
    28081 gaagaatcct taccggtata tgaagcatta gctagtaaaa caaggatcaa gatcatccag
    28141 ctactttcca aaaaaaagat gaatgtcaaa gatttggcaa aagaacttgg agtgagcagt
    28201 gctatcacga cgatgcacgt gaaaaagcta gaggaagcaa acatcatcaa aacagaaaaa
    28261 gtaggacaac aaaaaatttc gagccttcgt gtcgataaaa tcgatatcag ttttccagaa
    28321 aaaatattca atgcttttga tacgaaagaa acatcgatac ctattggtca ctatacaaat
    28381 tatgcaattg aaccaacatg cggtttagct acgatccatg attttatcgg gaaagtggat
    28441 gaaccacgct attttatgga tcctcgaagg atggatgccc gcattctctg gtttaccagc
    28501 ggatttgtcg agtatcaggc acctaatttt ttaaatacag aggatacatt ggagatgttg
    28561 gaagtttctg tggaaatttc ttctgaattt cctttttcaa atgacaattg gccttcagat
    28621 atcactttca gcctaaatgg tgtggagcta ggaacatgga cgagccctgg agattttgct
    28681 gatatccgcg gtaaatatac gcctgattgg tatccagata atcttaatca atacggcttg
    28741 ctgaagacga tccgaattac caaacacttg acgaatatga atggagaacc cttgtcaaac
    28801 atcacgatca acgacatccc taaagagcaa gacacttggc atttgcgaat cgaagtgaaa
    28861 gacgatgcca aacatgtggg gggctgtact ttatttggta aaggattcgg taattacgat
    28921 caggatatca aattaaaagt ttactacagc tgatattttt cttttctatt ttataaaaca
    28981 aaaacagcca atccctatga agggacggct gtttttgctt tattgttatt tccagaaatc
    29041 atccaaaacg gaaattggca aatgtcgttt atgttgtgtc ttgttccacc atgcttcgat
    29101 tttttcttgt gcttgagcgg gaacttcttt tccttctaga taatcatcaa tttcattata
    29161 tgtgacacct agtgcgactt catctgcaat cattggtttc ccatcttcaa gatctgcagt
    29221 cggaattttt gtgtataatt tttccggtgc attcaatgct tgaagcaatt gtttcccttg
    29281 tcgtttgtcc aaacggaata atggcaaaat gtcggctcca ccatcaccaa atttcgtgaa
    29341 gaatccagta atgttttctg cagcatgatc tgtccctaat acggctcctg ctctttctcc
    29401 agctacggca tattgcgtaa tcatacgttg acgtgctttg atatttcctt tgttaaaatc
    29461 gctgattccc acacctgctt cgcttaatac ttgtacttgt gcatcaacag ctggtttgat
    29521 atttactcgc agactaacat cgggctggat aaagtcaaga gcagcttttg catcttcttc
    29581 atctgcttgt tcaccataag gaagacgaac agcgataaat tgatagtttt catcttttgt
    29641 ttcttcacgc atttcttcca tcgttagttg cgccaaacgg cctgctaaag tagaatcttg
    29701 gccgccgctg atacctagta caaaagtttt aagaaacgga tattttttca aatatgcttt
    29761 cataaagtca atacttttac gtatttcttc ttttggatca attgttggtt tgacacctaa
    29821 ttcattgata atttctgtct gcaggctcat ttattctcct cctatgcttc tgtatgaagt
    29881 tctgctacgc tttttttcat tcgttccatg atagccattt tatggttcca gcattcagta
    29941 gacagatcga ctgggtattt ttgcggattc aagtctcgtt tatactcttc ccatagtgaa
    30001 tctagatttt cttttgtata ttctttgatt tcgtttagtg caggtaattc gtaaactctt
    30061 tttccttcga taaagatatc ttgtaaaact gggcgagctg taaagtctcg aacagtttta
    30121 ttgataaacg tatgcactgg atggaacatg aagatagctt cttcttctcg agggtcttca
    30181 tcccataatg tcacgtagtc tccttcagat ttcccatcaa aatttcgagt gatgcgccac
    30241 acttgttttt tccctggagt agttactttt tctgcattgc ttgataactt gatcgtatcc
    30301 atcatttttc cttcatcatc ttcgatcgat actagcttga atactgcacc aagggctggc
    30361 tgatcatagg ctgtgatcaa ttttgtccca acaccccata catcaatttt agctttttgc
    30421 atctttaaat tcaaaatcgt tgcttcatcc aagtcattcg aagcatagat ttttgcttcc
    30481 gtaaatccgg ctgcatctaa ttgttcccga acacgcttag atatataggc catatctcca
    30541 ctgtcgatgc gtacgccttg gaagttgatt ttgtcaccta attctttcgc cacgcgaatc
    30601 gcacttggca cacctgattt caacgtatca taagtgtcga ctaagaacac gcagtcttta
    30661 tgggttttag cgtaggccat aaatgcttca taatcatttc cataagattg gactaacgaa
    30721 tgagcatggg tcccgctagc tggtatccca aatatcttcc cagcacgtac attgcttgtt
    30781 gcatctgctc cgccgatata tgccgctcta gttccccaga ctgctgcatc cagttcttgt
    30841 gcccgtctag ttccaaattc caatagtgga tcttctccga tgacagattt gatacgagct
    30901 gcttttgtcg cgatcaacgt ttggaaattg accatattca acagtgctgt ttctactaat
    30961 tggcagtgcg ctaaaggccc ttccacttgg attaatggtt catttgcaaa aactagttct
    31021 ccttcacgag cagaacgaac tgttgcttta aattcaaaat tttgtaaata ctccaaaaaa
    31081 ccttcaggat agacttctaa tccacgcaag taagcaatat cggaatcatt gaacgtcaga
    31141 ttctctaaat aattgactaa ccgttccaat cctgcaaaaa ccgcgtatcc gtggttaaaa
    31201 ggcatttctc gaaagtaaca ttcaaaaaca gcatgtaaat cagctcgacc taattcccag
    31261 tatgtctgca tcatattgat ttgatataga tccgtatgca gcgtcaaact gtcgtctggg
    31321 taagtattat tcatcttgta ttactcctct tggcaagttt tcatttccta cccattataa
    31381 caaaaaaaat taataggaaa agaaatcttc cattttttct gaaaaagcac atgttttacg
    31441 gatatttctt gctaaaaatg aactccatct gctaaaacta tcctttctta ttggtattta
    31501 acctaaacga gaaagaatac ttattttatt caatgtatcc gagacacgaa gaaattagcg
    31561 ttttctattt ttcttacttg ataaaataag tagattttcc tactacaatc gtagccgtta
    31621 agataccgat gattgaacaa atataaccaa atgtctctac aacataatca atggcaaaag
    31681 tttttgaagc aataatacgt ctttcctttt ttgcttctta tccccaaaga aacggatata
    31741 acttattatg tattgaattt ttttggaaag gcgaattatg acaagatata ccttgcgttg
    31801 aaaaaactcg cggaaaaaat atcacacgtt tttaaaaaga tacaaagtaa gaaagcagct
    31861 gtcaatagaa tggaaacaaa aatggcgaga catcaatcag tctcgccttg ttttttattg
    31921 tatacattag atatggagca ggttaccata aagatgatta ttagtcgtta ttaaatagaa
    31981 tccttcgtat ttttttgttc tatcactaca atatctttat ttggacgttt gaattgtatt
    32041 ccggcacgtt ctagctcctg tttcactcct acatggactt tgacatcttc tcgtgtcatt
    32101 tttaaaacgt gatgaatcgc atagtcgatt tcttgccttg tgattttttt cttcgtattg
    32161 atcatcacga cttgcttttt atcatatgtg tctaccaata gcagttgtgt tcgatctaac
    32221 gaaaataatt tgtaataatt aatagccgta ttcccaaaaa agaattccac ta
//
LOCUS       DAYYME010000018        32207 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000018 DAYYME010000000
VERSION     DAYYME010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.018"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(145..525)
                     /gene="yajC"
                     /locus_tag="ACHBFW_000679"
     CDS             complement(145..525)
                     /gene="yajC"
                     /locus_tag="ACHBFW_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFM9317019.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(622..1767)
                     /gene="tgt"
                     /locus_tag="ACHBFW_000680"
     CDS             complement(622..1767)
                     /gene="tgt"
                     /locus_tag="ACHBFW_000680"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFM9317020.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1985..2683
                     /locus_tag="ACHBFW_000681"
     CDS             1985..2683
                     /locus_tag="ACHBFW_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM9317021.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2851..5490)
                     /locus_tag="ACHBFW_000682"
     CDS             complement(2851..5490)
                     /locus_tag="ACHBFW_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9317022.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6109..7140)
                     /gene="queA"
                     /locus_tag="ACHBFW_000683"
     CDS             complement(6109..7140)
                     /gene="queA"
                     /locus_tag="ACHBFW_000683"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFM9317023.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7333..8508
                     /locus_tag="ACHBFW_000684"
     CDS             7333..8508
                     /locus_tag="ACHBFW_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9317024.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8512..9153
                     /locus_tag="ACHBFW_000685"
     CDS             8512..9153
                     /locus_tag="ACHBFW_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9317025.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9150..10067
                     /locus_tag="ACHBFW_000686"
     CDS             9150..10067
                     /locus_tag="ACHBFW_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9317026.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10064..10735
                     /locus_tag="ACHBFW_000687"
     CDS             10064..10735
                     /locus_tag="ACHBFW_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9317027.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11276..11764)
                     /locus_tag="ACHBFW_000688"
     CDS             complement(11276..11764)
                     /locus_tag="ACHBFW_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFM9317028.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11965..12231)
                     /locus_tag="ACHBFW_000689"
     CDS             complement(11965..12231)
                     /locus_tag="ACHBFW_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM9317029.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12231..12494)
                     /locus_tag="ACHBFW_000690"
     CDS             complement(12231..12494)
                     /locus_tag="ACHBFW_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM9317030.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            13015..13155
                     /locus_tag="ACHBFW_000691"
                     /pseudo
     CDS             13015..13155
                     /locus_tag="ACHBFW_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(13297..15120)
                     /locus_tag="ACHBFW_000692"
     CDS             complement(13297..15120)
                     /locus_tag="ACHBFW_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM9317031.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15718..18423)
                     /locus_tag="ACHBFW_000693"
     CDS             complement(15718..18423)
                     /locus_tag="ACHBFW_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFM9317032.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18729..19676)
                     /locus_tag="ACHBFW_000694"
     CDS             complement(18729..19676)
                     /locus_tag="ACHBFW_000694"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFM9317033.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19866..20135
                     /gene="rpsN"
                     /locus_tag="ACHBFW_000695"
     CDS             19866..20135
                     /gene="rpsN"
                     /locus_tag="ACHBFW_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM9317034.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20311..20673)
                     /locus_tag="ACHBFW_000696"
     CDS             complement(20311..20673)
                     /locus_tag="ACHBFW_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM9317035.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20684..21805)
                     /gene="alr"
                     /locus_tag="ACHBFW_000697"
     CDS             complement(20684..21805)
                     /gene="alr"
                     /locus_tag="ACHBFW_000697"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFM9317036.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21821..22171)
                     /gene="acpS"
                     /locus_tag="ACHBFW_000698"
     CDS             complement(21821..22171)
                     /gene="acpS"
                     /locus_tag="ACHBFW_000698"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFM9317037.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22301..23812)
                     /locus_tag="ACHBFW_000699"
     CDS             complement(22301..23812)
                     /locus_tag="ACHBFW_000699"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM9317038.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24134..25486)
                     /gene="murF"
                     /locus_tag="ACHBFW_000700"
     CDS             complement(24134..25486)
                     /gene="murF"
                     /locus_tag="ACHBFW_000700"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFM9317039.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25639..26715)
                     /locus_tag="ACHBFW_000701"
     CDS             complement(25639..26715)
                     /locus_tag="ACHBFW_000701"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFM9317040.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26911..28656
                     /locus_tag="ACHBFW_000702"
     CDS             26911..28656
                     /locus_tag="ACHBFW_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFM9317041.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28936..29373)
                     /locus_tag="ACHBFW_000703"
     CDS             complement(28936..29373)
                     /locus_tag="ACHBFW_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFM9317042.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29357..30730)
                     /locus_tag="ACHBFW_000704"
     CDS             complement(29357..30730)
                     /locus_tag="ACHBFW_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317043.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31052..31540)
                     /locus_tag="ACHBFW_000705"
     CDS             complement(31052..31540)
                     /locus_tag="ACHBFW_000705"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9317044.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31562..32164)
                     /locus_tag="ACHBFW_000706"
     CDS             complement(31562..32164)
                     /locus_tag="ACHBFW_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM9317045.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9208 a         6884 c         5943 g        10172 t
ORIGIN      
        1 aagttattta ttattattta tatgacacac ccgattattc aatattgagc aatatatccc
       61 atgctcttat ctctttcttt tttgaataaa aaatgaggta ggacatcttt ctgccctacc
      121 tcgtctttaa ataagctttg atacttattt ttcttccttc tcttctgtct cttccagcgt
      181 ttctgttgtt tcagttttta cttcaactgt ctcagctggt tcttctgttg tttcaacagt
      241 agtcgttgca tcatttgtta cgacagtccc tggtttgacc gttttgattg ctgcacgatc
      301 gtactcaagg aagatccctt cgcagtctaa aacaactgtc cgtttgtcat tgtcgatttc
      361 tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa tcacctggtt tcatgctatc
      421 taacaagttt tgacgttctt gctgttgttt cttttgcgaa cgtgacataa aaaaccacat
      481 tgctaatagc gcgataaaca tcacaagcat tggtaatgtt cccatttttt tcacctcact
      541 aattctgtat ctacaaaaaa cactttagca gaaaaatgct gataacacta gttttttact
      601 taatttttaa aagattttcc tttagaagct ttttgcgttt tctttattga acccgtattc
      661 ttcaaaaaag gcttctctaa attcaagcaa attatcatcc atgattgctt gacgtacttg
      721 tttcattaga ttcaacaaga agtataaatt gtggtaagaa gtcaaacgaa tcccaaatgt
      781 ttcatcacat ttgatcaaat ggcggatata ggcacgtgta tagtttttac atgtataaca
      841 atcacatttc tcatcaattg gacggaaatc atgggcatat ttcgcatttt tcactactag
      901 acggccttga gaagtcatac atgtgccatt tcgagcgatt cgagtaggca agacacagtc
      961 aaacatgtct actccacgga taacaccatc gatcaatgaa tcagcagcac cgacacccat
     1021 caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg aattctaata cacgattcat
     1081 ttctgattta ggttccccga cagataaccc gccgattgag taaccaggga aatccatccc
     1141 gactagatct ttcgcgcttt gacgacgaag atcttcaaac cctgctcctt ggatgattcc
     1201 aaataatcct tgacggtctg gattagcatg agctttcaat cctcgttctg cccatcgaga
     1261 cgtacgttct actgattttt tcacgtagtc gtaactttca tcgaatggtg gacattcgtc
     1321 aaaactcatc atgatatcag agcccaattt attttggatg ttgattgctt tttctggtga
     1381 taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc acgccttctt cttcgatatt
     1441 ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa tctgttaaaa tcggttgatc
     1501 ccagttcata aatttatgca gtccgccagc ttcttctact aagtcttcac cagggcgtag
     1561 ccataaatga tatgtattac ttaaaataac gccagcaccc atttctttca actcttctgg
     1621 ggacattgtt tttactgtag ctaatgttcc tactggcatg aacattggtg tggggaaagt
     1681 tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta tgtttttctt tcttgattaa
     1741 acgatagcga atggcaggtt gcgtcattca tttccctact ttctatataa aatatcttga
     1801 caactcccac catcataatc gtttcaagtg ctttttgcaa gttccctcgg cttccttcta
     1861 ctttatttaa tattcttttt tttatccttt ttttatgaat aacaaaaaaa agaaaaacat
     1921 tatgacagaa atattttttg atacatggta tattggtgtt attaaagaaa aggagggaaa
     1981 aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa gagagtctaa aaggacgttg
     2041 gggacaagct gttttactaa atctaatccc tacattgatt ggtattgcta ctattcttct
     2101 tattatgatt cctattatct tgtttgccac caccatatat gatggatcag cgagcgtctc
     2161 agaagcgaca acttcaggag gcggtagttc aggaggcggt ggcggaatta tttccagcat
     2221 cattagcgct ttgttcatga gcggtatttc ttggacttat cttgatattt tacgtggaca
     2281 aaaagatcag atcgaaccat tcaaagatgc atttcgtgga ttcaaaggtt tgtttatcgg
     2341 cggagtgatc agcttagctg tgctgatctc gatctttaca acattatggg gtttgctatt
     2401 agttattcct ggaatcatta aaagctattc ttattctcaa agctatttta tttactacga
     2461 tatcgtccaa gaaacaggac aaacacctaa agtactggat acgatcactg caagtcgaaa
     2521 attgatggat ggttataaag gacaactttt ctggttagat ctttcattta tcggctggca
     2581 tatcttagca ttagcaactg ctggtattgg ttatctatgg ctcagcccat acatttctgc
     2641 aacaaaagca gcattttatg aacaattgcc aaaagatatt taaaagagag tgtgacttgt
     2701 gaccgagggg gtggcaggag aacaccgttt atcgagaggc agtaaaagaa acattcagcc
     2761 cattagcaaa aaggaggtgt gacaacattc atgtcacacc tcctttttgt atagttccag
     2821 taaaaagaaa atcaacttag aaacagttaa tcattcctct tcttctgctt ccggtaatcg
     2881 atggaacttg atcagttcat tcaccgcgat tggtacgata gataaaatcc ctaccaagaa
     2941 ccagtgattc aaactgatgt gtactaggcc gaatagttct tgcgtaaatg gcagcaaagc
     3001 aacagccgtt gtaatagcta atgccagtaa cgccatttct accaaagctt tattggtcgc
     3061 taaattgact ttgaagatcg attggctgct gcgtacattg aacacatgca gaatcgatga
     3121 ataagctaag atcaagaatg caacagtttg accgacatgc tgacttgcgt ctacccatgg
     3181 gctgatagta tcgacataac tacctaaata ataaccaaac aaggttacga tggtgaagac
     3241 ccctgcattt atggcaattt tttgcaataa acctcgagca aagatccctt catttttcgg
     3301 aatgggtggc tgatccatga tgccgtgttc cgctggctct cttcctaaag caaatcctgg
     3361 aagtccatca gcaactacat tgacaaacag tagttgcaca gctgtaaatg gtgctcccca
     3421 tcctaacagc atcgcaatca aaacaataaa gatctccgaa atattacagc ttagcaaaaa
     3481 gttgatagct ttacgaatat tctgataaac cgaacgtcct tgactgacag cgtctacgat
     3541 cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca ctttgcgcca catctgttcc
     3601 tgtgatcccc atggcacacc caacgttact tgctttcaac gctggtgcat cattcacacc
     3661 atccccagtc atcgctacga ctgcacccgt acgttgccaa gatttgacga tccggatctt
     3721 gtcttctggc gttacccgag catagacaga gagagctttg acacgcgcat ctaattcttc
     3781 gtctgataat tgatgcagtt ctgatcctga aagtgcttct gacggatcat tcaagatacc
     3841 taattctttc gctatggcac tggcagtcac cacatgatct ccagtgatca tgactgtttt
     3901 gatgccagcc tttttcgctc gcttgattgc tcctttactt tctggacgtg gtggatcgat
     3961 cattccgatc agaccaatca aacgtaagtc tttttccagt gcttcagaag aaatatcttg
     4021 cggttcttcg tcataagtag cataaccaac agcaatcact ctcagtgccc gtttaccaaa
     4081 gcgatcatta acgatcgccg cttgttctac atcgcctgaa ctaaagcgcg gagcaagaac
     4141 atcgaatgct cctttggtga ttgagatgta tttttcgccc atttggtgaa ctgttgtcat
     4201 catttttcgt tcagaatcaa aaggaagctc gtttactcgt ggatattttt cttctaattc
     4261 tgcctttgta tgataattct cttcaacagc ccttacgatg gctacttctg ttggattacc
     4321 tttgataaca aggtcgtcat cttcttgctc gaccgtcaca tctgtacata gtgcagccat
     4381 tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg atattcgtta cttcatcttc
     4441 tttgctccat acccggcgga cgcgcatttc attttgtgtc agcgttcctg ttttgtctga
     4501 gcagataaca ttagcggttc ctagcgtttc aactgctggc aactgtcgga tgatcgcatg
     4561 tttcctcgcc atcttctgca caccgtatgc aagtgtcaac gtaacaataa cagtcaaggt
     4621 ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg aacatctcaa ggagtggttc
     4681 tccttgcagt tcaccaatca caaatactaa agccgcagcc gctaaagcga tcatactgat
     4741 tcgtttgcct agctgattca atcggacctg taaaggtgtt ttttgcatag tattttcgtt
     4801 caaaagatcc gcgatcttac ccatttctgt agccatgcca atggcagtga cgatcgcttt
     4861 cccacgacct gcagcaaccg tacatccttt aaataccata ttaagttggt cacctaatga
     4921 ctcatcttct tcagcaataa atgctgcatc tttttcaact gcttcacttt ctccagtcaa
     4981 cgccgattct tctacacgta attgtgatgc ttcgatcaaa cgggcatcgg cagaaatagc
     5041 atcaccggac tctaaaacaa gaatatcacc cactaccagt tgctctgact caacagtggt
     5101 cactttgccg tctcgtatga cagttgccgt ttgtttattc atatcttgca aggattccag
     5161 tgctttttct gcgtttcctt cttggacaat cgccaatacc gagttgatca caacgatagc
     5221 gatgatcagc agtccttcaa ataaatcccc atgttctgta gcaaatgcgg tataaaagga
     5281 aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg gacaagcttc gaaagaattt
     5341 tttgatcatc gattctttcg gcgcctcatc gaatttattc aatccctgct tttttcgccg
     5401 ttcatccact tcttgtgaag acaacccagt atctggatct gcttctgtta ccttctttac
     5461 ttcttctatt gtctgcttgt attcttccat cctattcctc ctttaaaatc ttcgtacaaa
     5521 aaaaggtact tattttacta taacatcctt tttttattta cttcaaataa agggcttatc
     5581 gattatttgt taagaaagcg taaaatttaa agtgcttata tttgcaaacg ctaccaaagg
     5641 atgctattat atagatgtaa ggaaggagga taccatcaaa tagacagaag aatttctcga
     5701 tagaacagtg taagcaaaca aagttgttag aagtacaggt ttcacccaga catgcgtgta
     5761 gagtacaaac ctataaatag tgaatgcgta tggatgtttg gatcacgaag tatccgaaac
     5821 aagaagtaat gcgatacgta acaattgaat aacgtaaacg tctgaaaatt gaataggaac
     5881 aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa tttttgttta acatgaagaa
     5941 attcaacacc gtgctttgca tgtatgatgg aaagttacaa tcatttctgt ttatttgaat
     6001 ccttccgagg tacaagaaag ccgagcttat cgagagctcg gctttttctt tgtcataaaa
     6061 tctttgtaaa gcttttttgt tattcaaaac tagcggcttt tcataacctt atttcacgaa
     6121 catcgcatct ccaaaactaa agaaacgata gcgctcttca atcgcatgct ggtaagcaga
     6181 taaagtcaat tctcttcctg caaaggcact aactaacatg actaaagtcg attttggcaa
     6241 atggaaatta gttgagaaag cttctaccac tttgaattca tagccaggcg taatgaaaat
     6301 atcagtccat ccgctgtctg ctttgatttc tccattaaat ttcgttccaa tcgtttctaa
     6361 tgttcgaatt gaagttgttc cgacagccac gattttcccg ccatttttac gtacttggtt
     6421 caatcgctca gccgcttctt ccgtcaaacg gtagaattca ctatgcatat ggtgttcttc
     6481 aattttatct acgctgacag gtcggaatgt acctaaccct acatgtagtg tcagatagac
     6541 taattcaaca ccctttgctt cgatctcatc aagtaattct ttcgtaaaat gcaatccagc
     6601 tgttggcgct gcagcagaac cattttcttt cgcatagaca gtttgatagc gatctgggtc
     6661 ttccagacgt tctttgatat atggaggcaa tggcatttct cccaatgatt caagaatctc
     6721 tagaaaaatt ccttcatact caaagtcaat gatccgtccg ccgtgttcca attcttcttt
     6781 cactactgct tgcaatctgc catcaccaaa agagattctt gtcccaactt tggcacgttt
     6841 cgcaggtttg atcaatgtct cccacgtatc tccttgtgta ttcgttagaa gaaggacttc
     6901 taaatgcgca ccagtatctg gtttttctcc atataaacga gcaggcagaa ctcgtgtatt
     6961 attcataact agcgcatcac caggctgtaa ctcttcaatg atttcatgga aatgtttatc
     7021 ttccatctct ccagttttcc gatccaaaac aagtaaacga gaatgatcgc ggtcttttaa
     7081 tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca aaatcttctg ttgttaacat
     7141 atcgtcactc actttctatc atgtgcttca tcattctatc atgtttttcg ttctgttggc
     7201 aaaatcagca ctttttcact tctatttttt gttttcctaa ataaaaaaac taccctattt
     7261 gtattttttc attgttttag gcatactagt aatagatagt tcttcattga aaatagaaaa
     7321 agaggtggca ccatgattga atttcaaaat gtgtcaaaaa tttataaagg tggaaaagtc
     7381 gctgtagagg acgtcaatct ttcatttgaa aagggtgagt tcatttgttt tatcggtacg
     7441 agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc gaatgactga tccaacacaa
     7501 gggaaaattt tgattgatgg aaaatccatc caagaaattg atccagtgga attgcgacgt
     7561 cagatcggct atgttatcca aagtattgga ttgatgcctc acatgacgat ccgtgaaaat
     7621 atcactcttg tccaaaaatt gttgaaagtc agtcaagaag aacggaataa aacagctgaa
     7681 aaaatgattg atcttgtgga attgccccgt gagatgttag atcgctaccc acatgaatta
     7741 tctggtggtc agcagcagcg tattggagtc gttcgtgccc ttgcagcgaa tcaagatatc
     7801 atcttgatgg acgagccatt cggtgcgctt gatccaatca ctcgtgattc cttgcaagac
     7861 cttgtcaaag atttacaaga gcgtttggga aaaacgatcg tatttgtaac ccatgacatg
     7921 gatgaagcga ttaaactagc aagccgtatc gccatcatga gtgaaggccg tgtgatccaa
     7981 tttgatacac cagaaaatat tttacgtcat cctgcaaacg atttcgtaga agagttgatt
     8041 ggagaagatc gcctactaca agccaagcca aatgcgacaa gagtcggcga agttatgcta
     8101 aatacagcaa tcacgattac accggaaaaa tcattacaag aagcaatcaa actgatgagg
     8161 gaaaaacgtg tagatacgtt gttagttacc gataattcga atgtattaaa aggatttatc
     8221 gatgtagaaa cgttgaataa aaaacgagga aaagtatcca gtgtcggcga cattttgaat
     8281 aaacatgttt tttacgtaaa agaatccgcc tatcttcgtg ataccttgca acgcatttta
     8341 aaacgcgggc tgaaatacgt gccggtcgtt gatgaacaga acaaagtcgt aggtatcctg
     8401 actcgtgcat cattagtgga tatcgtctac gatgtgattt ggggagaaga tcccgcaact
     8461 actgatgttg cagagtcagc tactcctgaa gaatctaagg aggtctgacc tatgcagcag
     8521 ttttttcatc agcacggttc agaaattctc tcaaaaggtt ttgaacatct ttatatttca
     8581 ggacttgctt tgatcattgg tatcatcatt gctgtaccgc ttggtgtctt actgacacgt
     8641 tttccaaaag ctgccacgat tgtgattggc ttaaccagtg ccttgcagac aatcccttct
     8701 cttgctttat tggcattgat gatcccgcta tttggcgtag gtaaattacc tgcgattatc
     8761 gcactcttta tttattcatt attgccgatt ttacgtaata cgtacattgg gatgaaaaat
     8821 gtcgattgga attatcggga tgtggcaaaa ggcatgggga tgactgagtt ccaatccatt
     8881 ttttccgttg aattgcccat tgctatgcca acgatcatgg ctggtattcg tttagctgcc
     8941 gtttacgtga ttgcatgggc tacacttgcc tcttatatcg gcgctggtgg tttaggcgat
     9001 ttgattttta gtggactgaa taattaccaa ccagatctca ttttggcagg tacgatccct
     9061 gtaacaattt tagcactatt ggctgattgg cttctaggtc tcttggaaca ccggctgaca
     9121 ccgatcgcac ttagagagga gaatgaagaa tgaaacgtat actaaaaatg accatggttc
     9181 ttttctctct tcttatactt gcgagttgtt ccttccctgg tcttgccagc aatacagatg
     9241 aagacaccat ttcgatcaca ggtggaatca cttcagaagc acagatctta gcaagtctag
     9301 ttgccggaat ggttgaacat tatacagata aaaataccgc aatcatcaac aatctggcta
     9361 ctacaactat caaccatcaa gcgatgatga acggagatgc ttcaatttca gcggctcgct
     9421 acacaggaac agatttaacg actacattga accttcctcc tgaaaaagat ccgaaaaaag
     9481 cgtttgctac cgtgaaagat gaatttgaga aaagatacgg tcaaacgtgg ttcccgtcct
     9541 atagttttga aaatacgtat gtctttcttg ttcgaaaaga caccgcacaa aaataccatc
     9601 tatctaaagt cagcgatctg aaaaatgtag cggatgaatt agtagctggt gtagatactt
     9661 cttggatcaa tcgtaaagga gacggttatg acggcttcca agaaacttat ggcttctcct
     9721 tcaattcgat attacctatg cagattggac ttgtttatga cgcagtcgaa gcaggaaaaa
     9781 tggatattgt tcttggctac tctacggatg gacggatcgc cagttatgat ctagtcatgt
     9841 tggaagatga tcgtcaattc ttcccaccat atgatgctgc tccagttgta gacaacaaga
     9901 tattagctga tacgcctcat ttaaaagaag ccttaaataa gttaggtcat accatcagta
     9961 cggaaaaaat gcaggaattg aactacgaag cagataataa tctagtggaa ccatcagtag
    10021 tagcacaacg tttcttacaa gaaaaccact attttgaagg gaagtgaagc aattggataa
    10081 aatgaatgtc tttcaacaat ttttctatta tttccaggaa aatggctcct atatctttgc
    10141 acaatttatc cgtcacttcc tgatttctat ctatggtgtc ctctttgcag cagttgtcgg
    10201 gattccggtt gggatcatga tttctaggag acgtaaatta gcaaactggg tgattcgttt
    10261 ggcgaatatc atccagacga tcccttctct agccatgatc tccattttaa tcattggatt
    10321 aggcctggga gtcaatgtcg tgattgttac tgtatttttg tattccctct taccgatcat
    10381 caagaatacg tataccggga tgatccaagt agacaaaaac atccttgatg tcggcaaagg
    10441 catggggatg actgctagac aacgactatt catggtagag ttaccactct ctgtttctgt
    10501 tatcatggca ggcataagaa acgcgttagt tgttgccatc gggataaccg cgatcggtgc
    10561 attcgtcggt gctggtggtc ttggagatat cattatccga ggaaccaatg caacagaggg
    10621 cacatcgatt attttagcag gtgcattgcc aactgcatta atggcgatca tcacggactg
    10681 gcttttaggt attttggaaa gacggctaga tccagccagc cgtacatcac gctgatgtta
    10741 ttgtaaaaaa caaaagcttc tgcgaccatt tgttggtcgc aaaggctttt ttagttctgt
    10801 catttttaag gcaataatcc actctgattc ttccctcctt atgggcaaag agttgctttt
    10861 cttagctatt tatccattcc gtatactcca acttcatact gccaacgggt tcatatctgc
    10921 atccaccgac taagtttttt tcttatccgc taaggcattt tgccaatcca ttttcccatc
    10981 actcgtcatg gaattcatac cacatgatct ctaaatactc gtctgcttgt tctttgaatt
    11041 gggcatcaat tcttacatcc tttggcgtgg ctttcattta agccttttcc atcagtcctc
    11101 gattgttcga tgccttcagc tgcatcatca aaactggtga ctgccgtatc ggtaacttcg
    11161 atcatctttt atacaatcgg gaacttgcca tcctcatcct tggctaaaaa catatctagc
    11221 cattccagct tctcgccatg ggaggtttgc ggttcctaaa accggtacac gattcttatg
    11281 gactgcatcc gtcgcaacta gacttggcga tacgatgatc cttcaccaga agagcacccc
    11341 cgtaaaccaa ttgatcgata tattgccaat aagaaaagac attggcgtaa aagatatcga
    11401 tccctcgtga tgcattgccg ttgatactgc tattcatggc cgaaagcgcc acgactttaa
    11461 cttcttccgt ttgagtgaca ttcatgttcg gcaatgtcgc tttggatacc cgctctgcta
    11521 aaggaacgac actggtattg tatttcgtat ctgtatcttt tccaaaagac catgccaacg
    11581 aatcctctgg gaaccaataa gacgactccg gttgattgtc cattgctttt ttcaacatac
    11641 ttttggctgt ttctcgataa acgacatttc caattgtctt cgcctcactt tggcgataat
    11701 cgatggtcgt tgccactgcc aacactaaca acgatgctcc aattttactg attttccttg
    11761 tcacacttct tcctccttgt tttacataaa cccgtcaaca ttccattcct aaactcaaac
    11821 aatgaccctt caaaggtgga gatataggaa caaattaaag aaatcatgca ataaatccaa
    11881 gaaggttggt tttagaacaa aaaaagagtc tcctttttgg ggagactctt tgttaactat
    11941 ctaaaaaggt tggctcttct cacttcactc ataatgtgtc caggcagaat aaatttctac
    12001 aactttattt tcctcatcaa ctttgtaaac cactcgatgc tgtctattca gcctgcgtga
    12061 gtatctgcct tcatgtgtag gtctcagctt ttcaaaagac tgcgtgggca tatatggatc
    12121 ttccttcaat gtctgaatca cttcttcaaa ttgtttcttt aaattcgttt gcttgatttt
    12181 tcttaaatcc actttcgcag agtttttgat tgcgactgta tagttactca tcaaagagct
    12241 tcccaatcaa tatcatcgat attcgtaaag ctactgtcgt ctttttcgcg gtctcgtact
    12301 ttatctaaag tacctgtttg ctcaagaaaa agggtttctt gaatcgctct ccagtcttcc
    12361 agcccaatca atacagcatt gtttccacta cgatcactaa taatctcgat ttcagtatga
    12421 ttttcattta cctctttcaa taactgataa aaatttttac gagcagtgct aggattcatc
    12481 gttgtattag ccattgtctc tcctcctttt tctgatataa ttatagcgta cttgattaag
    12541 tacgtcaaaa aaatacaata aagcacttaa attcagcaaa cttttcctct tttttacata
    12601 gagaaaaaga ccccttttta gggaggatct ttgtccacgc tcacttctta ctcctttacg
    12661 actagcggta gccaaacgta aagtaattgg taacaacgta tcatctttag caaaggtcga
    12721 atccaaagcc tgtgagtcta tgcaacaaca ccaatacacg aaggttcctc gttgcagtgt
    12781 gacttgattg atatcttgaa taacctgagg atacgcacga aagatttgta acggttattc
    12841 aaaactgatt ttggccgtca agtcttctac tgcctcaaaa atgattgcct ctttcgtctc
    12901 ctgtcattca tgttcactat cgatttgata cttgcttaga ccgttgggtt tgtgaatcac
    12961 caattaaaaa tggcactgag ttatggtaac aaaaaatggt cacttgacga ggctgagatt
    13021 cctacttatg aagacacatg gcacttaaga gtcgaggtaa aggaagaagc tgaaaatata
    13081 ggaggctgta cattatttgg caaaggattc ggaaattatg atcaagacat caaaatcaaa
    13141 atgttttaca gctaaatagg aagaataata acatttcata cgctgttccc atctttaatg
    13201 gcttatagag ggcaccttta gctgagaata agacaaaaca gaccctctag cttcaattaa
    13261 gagctagagg gtctgttttt gattttaaaa cttggtttat ttcttcaaat ggtagctata
    13321 ggtgctgaca acgatatttt cacgccaaga caatcgcaat cttaaccgca aacttgtttg
    13381 gttatggaga atattctgcc agcgtctgtt tggtacaaat tgaggaatca acacagtagt
    13441 tgtatagttt tgtttcgctg catttttact tacgatatct acataacgga taattggatt
    13501 ttcaatcgag cgataagaag agtggacgat tgaaaaacgg acatctggga aatgcttttt
    13561 aaattctgct tcgatttctt tttctttttc gacatcttcg tctagcgaaa catgcatcgc
    13621 aacgacataa tcaccgatcg aacgtgcgta attcaacgca ccgatattta cccgtgtcac
    13681 attcccgact aatacaagaa cggtattccc gtcataatca tgtagttttg catcattttc
    13741 taaacgaagc tgttctgcta cttttttata atgatcatgg atcttataaa aaatgaacat
    13801 aacgattggc ataatgatga agaaaggcca aatgtcccct aaccgataga caaacaaaat
    13861 cgcaatgatt gcatatgaga tgaacgcacc cgtaatattg gcaatcgatt tgcttaacca
    13921 tctttttcct tcttttttcc atttgacgac catccctgtt tgagataaag caaatggaat
    13981 gaacacgccg atagagtata acggaatcaa gcgttctgtt gagccatgga aaataaacag
    14041 caatacgatc gaacctagcg ctagtgtgat aatcccattg gaatagccta gtcgatcccc
    14101 acgatcttga tacatgtgag gcatgaattt atcttttgcc agattgtatg caagaactgg
    14161 aaaagcagaa aaaccggtgt tagcagcaac tgctaaaatc agcgctgtag caaattgaag
    14221 gacgtaatac aaaataccat gtccaaagac agctttacca atttgagaca aaacagtgac
    14281 ttctttttca ggaacgatcc cataccaata gttgataaat gtgatcccca cgaagaagaa
    14341 acctaagatc aatgccatca tcgtcaatgt agcagcggca ttttttgctc ttggcttctt
    14401 aaagaatggt acagcattac taatcgcctc caccccagtt aaagaggaag aaccagaaga
    14461 aaatgctcgt aaaatcaaag cgatagaaac acccggcaca gccgctcctg gaagtgcagt
    14521 agcttgcaat ggcacagcac ctgtcacgat attatataaa ccaacaacga tcagtaacga
    14581 gatcacaagg ataaatgtgt acaccggaaa taataggaac gatgcagatt ccctcagccc
    14641 acgaagattc agcatcatca gtagcaaaac gattgtcaca gaaatagcga cttgatgacc
    14701 gtagagtgct ggcaccgctg atgtgatcgc ttcagcacct gctgagacag atactgctac
    14761 tgtcaacatg tagtcgatca ataaagagcc accggaaatc aagccagcat tctttccaag
    14821 attctcactg ctgacgacat atgctccccc gccatgagga taagcatgga tgatttgacg
    14881 atatgatagc gttaatgaaa tcaataaaat aataacaaaa gcggcaatgg gtagcgaata
    14941 ccagatggct gcagcagaca aagcgactaa aacgacaacg atttgttccg taccatatgc
    15001 aatagatgat aacgcatcag acgacaataa tgccaaagca gcaaatcggg ttaatttgtg
    15061 ctcatcattt tcagcagatt ttaaaggctt acctactaac aaccttttta agtaatccaa
    15121 tttctaaact cctttacaca caattttact tcaagtttta tagtcgaaaa tttatagtaa
    15181 acaagatgtt atagtacgct cactgacctt aaaagtcaat aaactttaag aaaataattg
    15241 gaaaaaaaca gaaaaatatc aagcgctttt tcaaacgctt taatacagca aaaatctaac
    15301 atttttttat gggctttttc tttgaattct ataataaaag gatagaactt gacttagaaa
    15361 atcaagttct atcctttttg atatcttaac ttttggaaga gagatgtaag gctgctttct
    15421 acccatcatt cttactactc gaagcatagc gcagttgtca caacctcttg ataaacggtg
    15481 tttaaaagct tgcatctgcg gtaataagcc caaccaaatt aaaaacataa gaagctgttt
    15541 ttatttggtc gttcttatta cttgatgcag agcagctttt ttacaacctc ttggtacacg
    15601 gtgttcaatc agctgaccct cggtattagc tcaaaaaaac ggcaaaatat gaggaactat
    15661 ttttccattt ttttctctaa tactcaggtc aaaacactga tttcacaacc tcttttttta
    15721 atgaaaaata tggctctcat gcgccgcttc tttgaatttc gtcatttttt cttcgatgaa
    15781 cggatagatt gccgttccta cgattccaaa gacaacaaac aaaccgatca tgatccagat
    15841 tgctttcgta gcatttggcc aataaattcc tcctgcagat tcccgtaaca agtttaccgc
    15901 atgagtaaat ggcaagaacg gattgatcat ttggaagaat ttccctgaga cttggatcgg
    15961 atagttcccg cctccgccag agatcgacag tactaagatg ataatagcga tccctttacc
    16021 gacattatcg aataatgcag ccaagacata gacgatcatc atgaacgcta aggcgatcaa
    16081 gacagcaaat gacacgctgt agaccggatt ttggacatct actcctagta agaaaatatt
    16141 ccctaatgta acgatcaagc tttgcgcaat cgccattgtc aaaaatgtca gcatacgagc
    16201 aacgaattgt tctcgtttcg taaaattgtt tcggtctttc tttcctaaat agtattctgt
    16261 cgtagcaaca cttgaaagaa gaacagcgcc gacccacaga catagtgcag tataaaacgg
    16321 ggtgctagct gaaccgttat ttgcgattgg ataaagcgca ttggtttgta gctcaacagg
    16381 ttgtgtaaag aagtcacttt cttttgttgc gtctagtttc aataatttga tgacttcgcc
    16441 tagatcgact tcttgttcgc cttttcggat cgcgtctgct gctttttgga taccagcttt
    16501 gatatttggc cagtcattgt tgatcaagtc cgcagcagcg ttcacagctg tttcgacttc
    16561 tggcattttt tcatttactg ttgtcaatgt accggtgatt tcatttttta gtgctggcca
    16621 atcattttgg ataaaatcag cggccaatcc taatttttgc tgaatgatcg gtaattcatt
    16681 gttgtatagg tctgcccctt cattgatgcc attgacgatg gtatccatat ttccattcag
    16741 cattgtattg gcatcatgga tttcttgtcc aattgcaggc atttcttttt gatatttttc
    16801 taaaattgcc actgcattcg ttacagttgc tttcgttgag ctgagaagcg aatcaaagtc
    16861 gatctggtcc gctttgctca gtgcgccttg tgcagtccca atcgtatcga tgacttggtc
    16921 tagtagagaa gtgacagtgg aactgatttg acctgtattg ataccagcaa tgatgccatt
    16981 gatattatta acaacggcat caatttgatc tagcaattca tcaatagcct tcgtgtcttc
    17041 attttgaaca gctgtgttca aagcagatag gcggttttgc agattgctta gcaagggttt
    17101 taattgattt agctgatcaa taattggctg taatttcgta tttccagcac ttgtttgcaa
    17161 tttagtcagg aactcgacca gtttatcgat tgcattttgt tgttgagata ttccgtcaat
    17221 aaagccgttc aatacattgc tgatgttttt cttttcatca tcagtcagct taccatcttc
    17281 aatagctgtt cttacagtat ctattaacga acctgtcgtt gttgccactt gtccaattga
    17341 cttcaatgta acatctacac tgtttgtgat gcttggtatc gcatctttca gctgagaggc
    17401 acctgatttt gtttggtttg ctagatcact tgcctgattt tctaaatttt tcacatctgg
    17461 caagatatct tgtacttgtt ggatgatcgt caagccttgt tttgcttcat cgattccttg
    17521 gttcattgtc tcttggatcg aagcaaaatc gccgtcgatc tctgctagtt gcttaccggc
    17581 attttggatc tctgggattt tttcttgcaa atcaaggatg atttttgctt gatctttcaa
    17641 ctcaggcatt ttgtcattca atgcaacgac tttttcaccc atctcatcta ctttagggat
    17701 gtaatccacg aattcatttg ctttattcag tttttccttg atttcaggta gttgactttg
    17761 taattcgagg acttgttttg tgtagccgtc gatcgtgtcc aaattttcat ctgttgataa
    17821 aatcatatct ttaactttat tgatacttac taggttcgta tcgatgtcgt aacctatctc
    17881 attgaatact ttcaataaag tcgaactggc tgttttgata aattcatttg tgatttgcga
    17941 ttggatcgaa cttgctcctt tatcggtgat tttcggtgca atcgcattga ttttttcatt
    18001 taccgtatat tcgatttttg gtttcttgat atcaccacta gtaaaactca gcagatcttc
    18061 tgagaaatcc tttggcaagt aaatacctgc gtaatacttt ccggaacgga ctccgtcttc
    18121 taattgttct tttgaatcaa cgaattgcca tcctaattgt ttatttttat gaagcgattc
    18181 gatgacttgc tccccaatgg ccacttcttt accttgaaac tctgctggct tgtctgcact
    18241 gtaaacagca attggcagct ctccagtatt tccatatgga tcccataatg ctttgatgtt
    18301 aaaccacgca tatagcgaag ggatgatcat gatcgcgatg atcagaagtg ttgcaatcgg
    18361 gtttttgaaa atccgtttcc agtcaagcaa atataatcga agcgtatttt tgattgactg
    18421 catagttctc ccccttcttc ttttttcagc tgaattactt taccatgtat ttcatacttt
    18481 tttcaatcgg cttttttcaa aaaagtcgaa tttttggaca tatcacggca attgtctcat
    18541 tgtaatctct tacatattga gaaataatta aggattacag cctttacttt atcatgaaat
    18601 aaaagggaaa gataatttta tgccaaagtt tttatctatt atacgtatac gcaaaaaagc
    18661 taaaacattt tttgtctttg cccctacaaa aatgtttcag cttttttcgt tttttattcc
    18721 tgtatttctt atttttcgaa tgacaggatg attttcccag caggaggatg tccttcaact
    18781 agctgatgtg cttggatgac atgcttaagt gaagcagaca gattcattga aataaaaaca
    18841 tgaaaggctc ctttttggta ggctttgatt atgccagcaa aggcttctgc atctagatat
    18901 tcctttcttg gaacaaacga ttcataaaag ccttcctttt tttgccgcaa atctagatct
    18961 gggaggtcat ttaatgcaac atacctgccg cctggtttca tgatctggat gccggtttct
    19021 cctttgatac ttcctttcgt cgcatcgatt acaaggtctg cctgatcagc aaattgcaga
    19081 cctggatttg ttttatcata agcagcaaat gctgaagctc ctaatttttt taccttttcc
    19141 tcatttttac tggatgcact ggttaagatt cggattccct tttcatggag aagctggatc
    19201 agacttgacc cgaccgcacc tgatgctcct agtatcatta cagtatctgt cggctggatt
    19261 tccgtcaaat ggttgattag attataagcc gtaattcctg gcgtgaccat tccggctgct
    19321 tcttcccaac tcattttatc tggaattttt gctacttttg ctgatggcaa cacgacttct
    19381 tctccgtaag tcccgccgac tgcatggaca gctactcgat cacctacatg aacagttgtg
    19441 acatctgaac caatctctgt cacaatacca gcgccatcat tacctggtac ataagggaat
    19501 ttcaatgttc gtatctcttt catttctcct aaacgcaagg caacatcata aggattcaca
    19561 ctgaatgctt ttatttctac acgaacatgt ccagaaccga cttctctggg atgagcatcg
    19621 atttcttcaa aaacatcttg tgcttctccg taaccgttaa ttgcaaatct tcgcatggtc
    19681 accatctccc tttttcttta agtttacgtt tatgccttta ttttgacaaa aaaaatgtct
    19741 tttcgttttg gcgcatgatt caaaacaaaa tagttgtctt ttttcgcgta acgtgttaaa
    19801 ttatttatct aaaagcgtaa agattacgat ttacgctaat aaaaaataat tggaggaatt
    19861 tgttgatggc taaaaaatca aagattgcca aagcaaaaaa acagcaagaa acgattgaac
    19921 gctatgctgc tttacgccag gaattaaaag cgcaaaaaga ttacgaaggc ttgcgtaaac
    19981 ttcctttaga tgctcgtcca gaacgattga aaaatcgtga tttaatcgat ggacgccctc
    20041 gcggatacat gcgtaaattc gggatgtccc gtataaaatt ccgtgaatta gcacataaag
    20101 gtttgattcc aggagtaaaa aaagccagct ggtaatccaa gtctggctgt aatgtaaagg
    20161 aggagggcgc atggaccact ccttggatca tcaaaaaaga tacgttcgct tcttctagca
    20221 gaaatgctgg tactagaagc agacgtatct ttttttacgc acagctggtc cttttttata
    20281 tgtaaaggct gcatggttac ttcgctatat ttatttagta acaggagtca gtgcttcgat
    20341 acccacacta attttcaatg ctcggtcaac ttctgccata atgggtcgat ccaaatggca
    20401 gaccttttct ttcaatcttg atttatcgat cgttctgatc tgctccaaca ggataacgga
    20461 gtctctttct attcctgttc cagtagcttt gatcccaatg tgagtcggaa gcttaggttt
    20521 agccattttt gccgttatgg ccgcaacaat gatcgtcggg ctaaaatggt tacctagatt
    20581 attttgaata accaaaactg ggcgtgttcc gccttgctcc gatcccacga ctggagaaag
    20641 atcagcaaaa taaatatctc ctctttttac cataggatcc tccttattct tgatattctc
    20701 ttggtatccg ctgtcctaat ccgcaagcca cttcatagtg gatcgtacct aattgatctg
    20761 ctatatcctg catagtgatt tctttgtctg catttttgcc gataagcgtc acttttgttc
    20821 cgacaggaaa ttcttgaggc aatcggatca tcagttgatc catacataca cgcccaatcg
    20881 tttcacagta gttcccttct accaatagag aaaaaccctg catctttcgt ggccagccat
    20941 ctgcataacc gatcggtatc gttccgatcc actctgcttc aggagtgata tatgtttctc
    21001 catagccgat tccttcacct gctgggagtt ttttgacttg aatcaattca gaaaccagct
    21061 caagtgctgg ctgcaatgga taaacttccg gtagtgcgtg cccagaggga ttaagtccgt
    21121 acattgctac tccataacga atcatattgc cgattgtttc atcatgccat aaagccgttg
    21181 cactattgct gacatgtaca tatcgaggaa gttcgggaag ttttttcagc acttctataa
    21241 agcgttcttt ttgcagattc caatatgtat catctgcctg atctgctgta gaaaaatggg
    21301 taaataaacc ttcccattct aatacacgac tttctttgat gaatgcagct gcctctttta
    21361 cttcttcagg tgtacaaaat ccgatacgac ccatcccagt gtctgctttt agatgaatcg
    21421 aaagaggcgt ctctagctta cttttctcta aaacagcagc tgctgcttgc aaccattccc
    21481 gtgtacctgc agttactgat aaatcatgag caacagctac agtcagtgcg tcgtaaggaa
    21541 ccatattcaa gataaggatg ggctgagtaa atccagcttc tctcaactcg actccttcat
    21601 ctaaatttga aacacaaaag cccgaagctc cacctgcaac tgctgcctca gctgtttcaa
    21661 tagctccatg accgtatccg ttcgctttta ctactgcaaa caattctttt ccttgcggaa
    21721 gtcttgcaac ttcatttgct acattttcag ttatagcttt tttatgtatc actgctttcg
    21781 ttgggcggtg ccaagcgaca accatcgttt ttccttcttt ctagtcttcc aaaataattt
    21841 gtgcaaaagc tgtttcattt gtatgagtaa tactgaccca tacattgcct tcatgaggtg
    21901 aacgcgtcac aacaggctga ccattttcat tttttaaaat ctcgacatct tgaaaggtaa
    21961 cttttccgat cccagttccc caagctttag agaaagcctc tttgcaagcg taacgcccgc
    22021 ctaaaaattc tacttggcga tgaaaaggca aggactggaa tagtttcatt tcatcagagg
    22081 tcaagattcg tgcaatgaat tttggttttt cttcgatcag ccgagtgatc ctaggaagtt
    22141 cgactgcatc gatcccaatt ccttttatca tatgcttttc tcttttcaat aaatttagaa
    22201 cactctcatt ctaccaaatt ataaccattt tgccacaaat aagtccacaa ataacaaaaa
    22261 tgaaagtttc tcttaaaaaa gagaaacttt cattttatgt ttaatcgtta tttgaacgga
    22321 ttacaaatcc gcgtttttta tcattgtgac gtttaccgcc atctttttgg ctgttattat
    22381 ttttgctata accgcctttt gatttgtttc cacgataatt accgccgtca cgacgattac
    22441 ggttgtttcc gccaccacgt ttgccatttt tattgtatcc ttttttctgc attggcaatg
    22501 ggcgttctgg tgtgatcttc actggcactg catcagctgg atcttttgct gtcgttttta
    22561 gcaaaagtgc aactaaatct tgtgcagaat attcttctaa taatttatct gctgtttgca
    22621 ggtatttatc tagaccattt tctgcaagtt ttgtttcgat ttgttcaact gcagcaccta
    22681 attgaccttt gaatgcttct ttttcggttg gtggacgtaa agtcgtcatc cgttttttcg
    22741 tcaagttttc aatcacatgc aagtagtcca tttcatttgg tgtaacgaaa gtcacagaca
    22801 taccgccttt tccggcacgg cctgtacgtc cgatacggtg aacgtagctt tctggatctt
    22861 gagggatatc atagttgtac acatgagtga cacctgaaat atctaatcca cgtgcagcaa
    22921 cgtctgtcgc tacaaggata tctaaatgac cgctcttaaa tgaacgcaat acgctcattc
    22981 gtttttgttg agacaaatcg ccatggattc cttctgcacg gtaaccgcgt gcttctaatc
    23041 cgcgtgccaa ttcgtctaca cggcgttttg tacgtccgaa gacgattgtt aattcaggtg
    23101 tttgaacatc aaacaagcga gtcatgatat cgaatttttc gtattctttt gcgcggacat
    23161 agtattgatc gatcaagtca gctgtcattt ctttcgcttt gatcttcaca tgatgtggat
    23221 ttttcatgaa tttaacgcca atattcttga tagctggcgg catcgttgct gagaaaagta
    23281 atgtttgtct ttgatcagga acttgtgaaa tgattttttc gatatcttcc aagaagccca
    23341 tattcaacat ttcatctgct tcatccaaaa ctaatgtttg aacggttcca agtttcaatg
    23401 tgtggcggtt aatgtggtca agcatacgtc ctggtgtacc cacaacgata tgtggacgat
    23461 ctttcaagcc acggatctga cggccaatat ctgctccgcc gtaaacggct tgtacacgta
    23521 cttttttgtc gcgccctaaa cgatataatt cttcttgtgt ttggatagct aattcacgtg
    23581 ttggtgcgat aactaaacct tgtaattcgt gacgatctgg atcgattttt tctaacattg
    23641 gtaatccaaa tgcagctgtt ttccctgtac ctgtctgtgc ttgtccaata acgtcttttc
    23701 cagctaatgc tagtggaatc gtttctgctt ggataggcgt tgcttcttca aaaccagcgc
    23761 gttcgactga tttcaacaat tctggtgata attctagttc tttaaatttc aaatgaatcc
    23821 tcctatatgg ttgttaactg aggatgtagc ttttttgcta ccccgtcgat ttgttcggtc
    23881 aaagtgtatt gtttcttttt gacagtggcc acgcacgttt ttatccattc ttagcccctg
    23941 tggcaccacc gaaaaatcac tttgcgaagt ttaggcatta gaaaagccaa acgggtcgcg
    24001 ttcagcttta tcattattat gacatctggc ttcaacaagt tgtttctatg gcaaaaccag
    24061 tccaatttct cttcatcttt cggcaatagg ttattctaac atagtttttc gttttctgca
    24121 agtttacaga tttttatttc atttgctgaa gttgttctac cacttcaaat aagcccatgc
    24181 tgttgctgcc ttttagcata atactatctg ttgcttcaag attttgtttg atagcatcaa
    24241 tcaatttatt cttttctttt tcaaaataga acacaggtag ttgggggtat ttttggttca
    24301 attcttcttt tagtgcatgc atttgttccc cataaaggaa aatcatggcg aatttctcag
    24361 gatcgatatg ttcactcatt tgcgcatgca tatttaaaga ctctttccct aattccagca
    24421 tatctgctaa cacagcgatt ctgcgtcctt ttgttggcag atttttaaaa gtatctaata
    24481 ccagtcccat ggctgttgga tttgcattgt agacatcgct caaaatagct gcaccatttc
    24541 ctgccgttag ccattcggtt ctgttttgag tgatagaaac atgggcgagc ccttggcgta
    24601 tctcaggtat ggacagtcca aagaagcgcc cgatcgtata cgcaatcaaa gcattcgtta
    24661 cattataact tccaggcagc gggatcgtaa atgtttctcc ttctactaca aaatcggttt
    24721 gtgcttttcc ttttgctgtc acttccgcat gaacatcgcc atttccaata ccgaatgttg
    24781 tgactgtttg tactaatgtt gccgttaaag gtgtcagcaa cggttcatct gcgggcacaa
    24841 cgagtaatcc gtctgataca agtccgtcag tgatttccat tttggcttta gcgattcctt
    24901 ctcgcgaccc aagattttct atgtgtgctt caccgatcat tgtgattgct gcagcttttg
    24961 gttgagccat aagtgaaagt tccgtgattt cattggcgtg atccatgccc atttccaaaa
    25021 cgatcttttc tgtatcttgg ggcatgtgca aaatcgtgta aggaagacca atattattgt
    25081 tgtaatttcc ttgcgtctta tatgttttgt atttttcaga caagacagac gcggttagat
    25141 ctttcgtggt ggttttacca ttgcttcctg taattgcaac gacttctgcg cctacttttt
    25201 ccaagtaata ggttgccaat tgctgcatgg ctttcaaagg atctgctaca ggaataactg
    25261 gaaaattttt tggagcttgt tcaacaggca agctccaaaa tgaagcagat gctccgtttg
    25321 tgatcgcttg ctggataaat tcatgtccat ctcgcgtacc agcaagagga acgaataaac
    25381 ttccttcggt tatcttccgg ctgtcaaatt cgacattggt aatctttgtc agtgcagagc
    25441 attcgctgcc cacggcttgt gcaatttctt gtattgttag attcattgag atctctccta
    25501 aaaaaggttg tgaaaaaagc gtttggccta aacggcgggt tcgcatgact ctcacttcca
    25561 agtaaatttc tctattcgta tttagaagct agaatgaacg cggttacgca atcactccag
    25621 cctcttcttt atttctcttt attcattttt ttcaaaaaaa gattgacgct gatggtatcg
    25681 attcattcct aactggatca gttcttcaat caaatcaccg tatttcaagc ccatattttc
    25741 ccataagagt gggtacatac tgaactccgt aaatcctggc atagagttta attcattcag
    25801 gaataattca tttttatttg tcaaaaagaa atcgcaccgg ctcaatccgc ttccacctaa
    25861 catcgtgtaa gctaacttcg cgtactcttg cgctttttga taaacttctt ctggcacttc
    25921 ggctggaatc tgcatttcga ttttattatt gatatatttt gcttcataat catagaatgc
    25981 tacgtctttt acgacttcac caggcaaagt cgtccgaacg tcttcatttc ctaatacagc
    26041 aacttcgatt tcgcgcgctt caattccttg ttcaacgatt gctcgagaat catactgata
    26101 ggctgttgct aaagcatttt gcagctcttc tcggttttct gcttttgtaa tgccgacact
    26161 agaacccata ttcgccggtt tgacaaacat cggataaagc aaagaacctt cacattgatc
    26221 aaatactttt ttaggatttt ctttccattg attcttaagt actggtacat aaggaacttg
    26281 cggcacacca gcagcttgta aaatatactt ggtcatgatt ttatccatgg cacatgcact
    26341 ggtcaatacg cctgcgccga cataaggcat attcaatgtc tctaagaagc cttggatcgt
    26401 tccatcttcc ccgtttggcc catgtaaaac tggaaaaacg atggctcctt cttctttgat
    26461 ttcgcccgga ttgatcactt ttcctgtaaa gccttcctct gtctgtccac ttgggtccca
    26521 tgaaagatgc aagacatctt tgctggcagg tttttctgtt agtaatggac ctttgaccca
    26581 ttgtccttct tttgtaataa atacgagttg aacttggtaa taattataat aaatggcatt
    26641 taaaactgaa aatgcggaaa gaatggacac ttcatgctct gcgctgcgtc cgccatatag
    26701 taaagtaatc ttcaaaagat tgtcctcctg tattctaaat cgttcagtga tttactctca
    26761 tattttagca caaaacaaaa ataaacgaat aacttattta taaaaaaacg agccagcttc
    26821 taaaagaacg agcgtcattt attttttgtc agctagatta tttgtcccgt tttttataaa
    26881 aagttatgat aaagaaaagg agggatgatg atggcgcaaa caaatccacg cagccgcttg
    26941 gcagaaatca ttcgagtgtt catcaattac aatgtagtgc caaactttgt acagcaaaaa
    27001 aatcccgaac aggtaaaaaa agcctttgaa gaactaggcc ccaccttcat caagatagga
    27061 cagatgcttt ctgttcgaga agatttgctt tcttctgcct tcactcaaac attcaaaaca
    27121 ttacaagata gtgtacctag cgacacattt tcaacggtca aaaagacgat cgaaacagaa
    27181 ctttctcttt cattatcaga tatattcgat gatttttcaa aatcgccttt tgcctctgct
    27241 tctatggggc aggctcatcg tgccaagctg aagaacggtg acgcggtagt cgttaagatc
    27301 caacacccta acatcgccga agagatccga cttgatcttc aactatttga acgagcgatc
    27361 cctttgatca aatacattcc tgaaactagc gtagttgatc taaaaggggt actccaagaa
    27421 gtaaaacgtt ccttgatcaa tgaaatggac ttcttaaaag aaagtcaaaa tggcgaacaa
    27481 ttctatcaaa agaacaatgg ctggaaagag atccgcagcc ccaagattta tgacgctttt
    27541 tgttcgaaaa aagtgatcgt gatggaagaa atgtctggta aaaacttgaa tcatttaatg
    27601 aacgccgaaa acaaaactga aacttttatc actggaattc aaaacaagca gctgaaacaa
    27661 gaagttgcaa agcttttagt tgagaacttt atgaaacagg tgtttgatga tggttttttc
    27721 catgctgatc ctcatccagg gaatctgcta ttccatgtac taacaaaaga agaacagaca
    27781 caagctagcc gaaaaactga aacagtccat gaaaaagagt tcggttcctt tgcttttcga
    27841 gcctccactt ctgcagaaga ccctgttgcg ccatatacca tcaactacat cgattttggt
    27901 atgatggggc atctttctgc aggacttagg caaaaactca cacaagccgt tttagcgctg
    27961 tatacgaaag atgcttatcg aatcgaaaaa gcggtccttc gactttgtca gcaagaaggc
    28021 tcgtttgatg aaagtcggtt ccaccaagaa ctgacaagtt ttcttgaaca gtattatgat
    28081 tctcctattg atgaaatcaa cttgcaagaa gtattcacac atgtcgtgac gatttgtcat
    28141 caaaataatc tgcagttcga tcgtgatatc acgcttttgc tgaaagcatt tgggacatta
    28201 gaaggggtga tccgcgtcct cgatcctgaa gtttccctca tggaagtcgc ttcgcctttt
    28261 gctcaacatt attttctcac tcatctggat gtagaagata cattgaaaca aagcggatta
    28321 gacttattgg aagggatgaa ggctgcgcct aagatccctc aacagctcca ccatctatta
    28381 gaaatgtgga cttcgggaca aggaaaagtc aatttggaat tgaaaaagca agacaaatta
    28441 ttgagcagga tcgaaagtat gatcaatcgg cttgttttcg gtatgatttt agctgcattg
    28501 atcgtagggt cctccctgct tgtccaagca gcacctgtag aaaacgcaga ggtcgtttcc
    28561 ttattaggga tatttacgta tgctattgcc gcattcgtca ttattttttt ggcaatcgat
    28621 gcattgatcc aaatgtataa gaagaggaaa aagtagaaaa aggttgtgac agaaagaatc
    28681 agcttcaaga aataagaagg ggttttacat aaattgtttc tcaaattttt gtgaaaattc
    28741 ggcttatttt tcgaagaggc tacttctgaa acaccgagaa taaaaaatgt gaaaaagctg
    28801 ttctgcaccc agtaataaga acaatcaaaa agggaacatg gaacacattt atcctgtttc
    28861 cctgttccct ttttgatgta ttcgagcata aataaaatac cttcgttacc tactatcttc
    28921 tgtacttggt aaaccttagt caattgcctt ttttaacgct tgacgaaaag acatatgatc
    28981 tttctccgta aagggttttg gaccctttgt ccttatgcca gcttttctta actttccact
    29041 atgataacgt tgttctagaa gcgtgccgat attcgcttcg tcaaattctt ttccagagtg
    29101 atggaacact cgcacaccct tcggcaatac aagtgaagct aaaccataat cttgcgtgat
    29161 caaaaagtca ttcttcttaa ccataccgac gattttataa tccgcactat cagctccgcg
    29221 gtctacgtag acgatttgga catgattagc gtattctttg gtcgtataat ggtccacact
    29281 ggtcacgata accacgtcaa gaccatagga ttctcctaat tttatgacat cttcttttac
    29341 tggagaacca tctccatcaa tgaatagcct cattcttttt cgccttcttc cataaatgat
    29401 aaacagttac taatccgctc agtaaaagac caacattgat ccattttgcc aagataagca
    29461 gttgtacact ttctatcggc actgattcat ggttcagcgt gatcaataat agatggattg
    29521 gctctcccat ttcttttgct aaaacaaaca aaagcgtcat caataaaaac tggtggatca
    29581 cactcccttt attttgagta gtatacttga caacgatccg gcgatccatt ggaatagctg
    29641 tatcagtttg ataatattcc ttatttagcc agctgcctgt tactacgtgc cagaaactaa
    29701 gcagaaagac acctatcgca aaaccatttg gaatcaagag gaccaataaa gaagaactca
    29761 gtccaagcaa ttgcatagcc aatggagacc atgttaaacg tttcaaaatc catggtccga
    29821 ctatttttgc tgcaatcaag cctgctgcat aaggcaaata cataaaaatc gaaagttttt
    29881 ctaatccgta cagtatgcca acataaaaac tgctaaataa aaaaaggaaa ttccctagca
    29941 ttccatttag aaaagtaaac agatttagcc agcttggcaa cttccattcc ttcttctggt
    30001 gtcctaaaac aaacacgaac aagatgaaca gcagcaagaa accataaata gcaagatcaa
    30061 aaagcctttc atcgaataac aatcgggcat ttctcaaaag aaacaataag ccaaagaata
    30121 tcaaaaataa aacaagctct cgcgtagcta ccagatttct tttgatgtct ttgaaatcca
    30181 gttcgtaacg cggataatga ctaaccgtat ggtaggccat gacgtaaaaa agtgtataaa
    30241 tagtaaagct tgctgcaatc tgagtcggtg caggcaactc aatcgcttga tacaggagaa
    30301 atagccagat agctgcaaat aagtactttt tcccatccat attgataaaa ccttgcgtct
    30361 tttcgtgaaa attgaccgtg gtattagcag gtacaagcca tgctgcactc agccctagca
    30421 aaacacttga aaaataaaat aaaggaaaat aaaatacgcc taatattcca agcaatgaac
    30481 cagcaccacc aatcagcaga gcgatcatca gcaacgtgta actatctaga ccaaatcgta
    30541 gactgttgac taaaaatagt ccagtcatcc gaaaggcata aaacagcgta aatggcaaaa
    30601 tcgtcagcca attatgcgag tctgttagtc ccaaaaataa aagataagga ataaaaataa
    30661 ccgtgttact taagataaag ggcgttgttt gtgtaaaatt acgcccaaat cgtttgagtt
    30721 tttcgaacat gacaatggct ccttaaacag tcttataact aatctgctct tcgattttag
    30781 cattaaaggg gaaataaacc aattttacag aatatattct tgtaaaaaaa aatacagaaa
    30841 gaagcataag cttctttctg catttttacg gtacttgatt actcaagcag acaagccctt
    30901 ctccacaaaa gtcttgcccg acacccctgc atccggcagg tgataaagat agaaatcaat
    30961 tatcctgtgc ctccaaaatc aattaggagc tctgtaaacc agagtccttc tcgacccgtc
    31021 gcataaattt actttaacac agttcctatg tttatgcgag agcttttttc attttataat
    31081 cgaccatttg aaatccttga ccgatatctg aaataagttc ttctgtaatc tcaaatcctt
    31141 catgcttata aacctcgatt gcttgatgat tcccctgatt cactcgcaaa aagatctctc
    31201 tttcctttgt ttgcgcagca agttcttcat accagcgaaa aactgaagaa gtcaatcctt
    31261 gaccacgtac tttatttagc aaatataatt tacttaaata aatttttcca tttatctctt
    31321 cataagcagt gtaaccgaca gtcttttctt cgctttttaa taaaaagtag gacacacctt
    31381 ctgtcagttc tttttgaatc tgtttttttg actgataagt tttcagcatg taatccactt
    31441 gtttttgacc aatgatcggc acaaaaactt ccggccatac ttcttgtatg actgccatca
    31501 gctcatccag ttcattttct gttactttta ttttttccat acacccacgt ccttttttac
    31561 attataaatg accttgcgct tgatggaata aaaagagcat gatcatgcta tacacaccca
    31621 ccgaccacgg tttcaagaaa atcacaatct gcataaattc cctaaaagat aatttcgtca
    31681 aaccagctac tagacagatc aagtcgtctg gcgcaaaggg aagcagcaag gtaacaacta
    31741 gtactttttt aatatctttc gtttttcttg tcagcaattc atcaaatttc tgatactttt
    31801 ttggcgataa aataagctgg acaaaggcta cgccatagta acggacaagc aaaaatccga
    31861 ttgcttcccc aatcactagg ccaatacaac tatataaaag cccaaatcca ttgccaaaca
    31921 tcagcatgcc cgctactgag gagatccctc ccggcaaaat aggaatgatc acttggatca
    31981 cttgcaacag aacaaatatc atcactgcat acttgccaaa ttgctggata aacgcttgca
    32041 aagacgtgct cgatcgaaaa atcccatgat gataagcata aagaatcaac acaaagaaaa
    32101 aaaacagacc aataatgggc atgatctgaa gacatttttg taaaactgat ccttttttag
    32161 acatctatat tccccacatc ttttttcatt tattaaaact gcctcct
//
LOCUS       DAYYME010000019        31469 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000019 DAYYME010000000
VERSION     DAYYME010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.019"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(782..1123)
                     /locus_tag="ACHBFW_000707"
     CDS             complement(782..1123)
                     /locus_tag="ACHBFW_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM9317046.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1123..1353)
                     /locus_tag="ACHBFW_000708"
     CDS             complement(1123..1353)
                     /locus_tag="ACHBFW_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM9317047.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1640..1978
                     /locus_tag="ACHBFW_000709"
     CDS             1640..1978
                     /locus_tag="ACHBFW_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317048.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2183..2745)
                     /locus_tag="ACHBFW_000710"
                     /pseudo
     CDS             complement(2183..2745)
                     /locus_tag="ACHBFW_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(3477..4058)
                     /locus_tag="ACHBFW_000711"
     CDS             complement(3477..4058)
                     /locus_tag="ACHBFW_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9317049.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4241..4867)
                     /locus_tag="ACHBFW_000712"
     CDS             complement(4241..4867)
                     /locus_tag="ACHBFW_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM9317050.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4889..6220)
                     /locus_tag="ACHBFW_000713"
     CDS             complement(4889..6220)
                     /locus_tag="ACHBFW_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFM9317051.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6375..6776)
                     /locus_tag="ACHBFW_000714"
     CDS             complement(6375..6776)
                     /locus_tag="ACHBFW_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM9317052.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6862..7068)
                     /locus_tag="ACHBFW_000715"
     CDS             complement(6862..7068)
                     /locus_tag="ACHBFW_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317053.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7163..8665)
                     /locus_tag="ACHBFW_000716"
     CDS             complement(7163..8665)
                     /locus_tag="ACHBFW_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFM9317054.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8683..9138)
                     /gene="argR"
                     /locus_tag="ACHBFW_000717"
     CDS             complement(8683..9138)
                     /gene="argR"
                     /locus_tag="ACHBFW_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM9317055.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9236..9853)
                     /locus_tag="ACHBFW_000718"
     CDS             complement(9236..9853)
                     /locus_tag="ACHBFW_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM9317056.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10074..11447
                     /locus_tag="ACHBFW_000719"
     CDS             10074..11447
                     /locus_tag="ACHBFW_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9317057.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11522..12634
                     /locus_tag="ACHBFW_000720"
     CDS             11522..12634
                     /locus_tag="ACHBFW_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9317058.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12706..14343)
                     /locus_tag="ACHBFW_000721"
     CDS             complement(12706..14343)
                     /locus_tag="ACHBFW_000721"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFM9317059.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14511..15119)
                     /locus_tag="ACHBFW_000722"
     CDS             complement(14511..15119)
                     /locus_tag="ACHBFW_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9317060.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(15503..16654)
                     /gene="queG"
                     /locus_tag="ACHBFW_000723"
     CDS             complement(15503..16654)
                     /gene="queG"
                     /locus_tag="ACHBFW_000723"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFM9317061.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(16673..17425)
                     /locus_tag="ACHBFW_000724"
     CDS             complement(16673..17425)
                     /locus_tag="ACHBFW_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317062.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(17566..18515)
                     /locus_tag="ACHBFW_000725"
                     /pseudo
     CDS             complement(17566..18515)
                     /locus_tag="ACHBFW_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
     gene            complement(18516..19355)
                     /locus_tag="ACHBFW_000726"
     CDS             complement(18516..19355)
                     /locus_tag="ACHBFW_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM9317063.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(19352..20098)
                     /locus_tag="ACHBFW_000727"
     CDS             complement(19352..20098)
                     /locus_tag="ACHBFW_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM9317064.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            20336..21706
                     /locus_tag="ACHBFW_000728"
     CDS             20336..21706
                     /locus_tag="ACHBFW_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9317065.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(21761..22657)
                     /locus_tag="ACHBFW_000729"
     CDS             complement(21761..22657)
                     /locus_tag="ACHBFW_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9317066.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(22771..22941)
                     /locus_tag="ACHBFW_000730"
     CDS             complement(22771..22941)
                     /locus_tag="ACHBFW_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317067.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(22938..23615)
                     /locus_tag="ACHBFW_000731"
     CDS             complement(22938..23615)
                     /locus_tag="ACHBFW_000731"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM9317068.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(23706..24611)
                     /locus_tag="ACHBFW_000732"
     CDS             complement(23706..24611)
                     /locus_tag="ACHBFW_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFM9317069.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            24786..26210
                     /locus_tag="ACHBFW_000733"
     CDS             24786..26210
                     /locus_tag="ACHBFW_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFM9317070.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            26256..27287
                     /locus_tag="ACHBFW_000734"
     CDS             26256..27287
                     /locus_tag="ACHBFW_000734"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM9317071.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            27294..28481
                     /locus_tag="ACHBFW_000735"
     CDS             27294..28481
                     /locus_tag="ACHBFW_000735"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFM9317072.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            28637..30019
                     /locus_tag="ACHBFW_000736"
     CDS             28637..30019
                     /locus_tag="ACHBFW_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM9317073.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            30056..30376
                     /locus_tag="ACHBFW_000737"
     CDS             30056..30376
                     /locus_tag="ACHBFW_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317074.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(30576..31244)
                     /locus_tag="ACHBFW_000738"
     CDS             complement(30576..31244)
                     /locus_tag="ACHBFW_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFM9317075.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8879 a         6386 c         5685 g        10519 t
ORIGIN      
        1 actcaaaata aaaaaaacct tacattatac tggaatgaac tgtattgata gtgtacctaa
       61 aagctctact gacataagat cgtgtaatcg gtaaagtaat tttattaatt atacgggatg
      121 taatcaatta gagaataaac taatcataaa aggtactttt acattcataa ttgtcgcttt
      181 gagattctac tttggttcac tatctgaccc aattgcacat tttcgatcat tacaagtatt
      241 ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt tttcagaatc tatgctaatt
      301 tctgtatgga tgttccagtg atggcaaatt tctcttttca ttgtttttct tgtcgcatga
      361 tatgcctttt tgtgttattc agttaaacta tagaaattct ttcctagaca gatcttgatc
      421 ccaaatttta cgtttttgag catcgatata gtttaaaatg acattactca tatctaaaga
      481 gtttagattt tttaggacta aaactgtcag ttgttctttt tcttctttct gttaaagtat
      541 tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa aacaagtttt gtgacacaaa
      601 aaaacgaatg acttgaggaa atcacctcaa tcattcgttt aaaatgctat atattcttta
      661 gtttaagata cggaaattaa tgccatcgac cacactagta gaatgaacga catttacttg
      721 gccttttttt aatttcgtta tgtttagcat ataaaaatat tatagagttt acttacaatg
      781 cttaaaacat atactgtata atctgatcaa ttttagccat gtcttgcaat ggtaagtttt
      841 ctattttttt gagttttcgt tccttaaaat caagagattt taattgtgta ataattactt
      901 gtccttttat atctagctct tcaggtaatg agtaacgtgt cggtaaattc tttatagtgg
      961 aagtaattgg acatatgacc gaaaacattg ttttacggtt aaattcataa cgagagacga
     1021 ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga gggatcaaaa tcaatccata
     1081 caatatcacc ttttttagga atataaattc tttcttctga cactataaaa tttctctccc
     1141 ttcaggagtt aaatcttccc attcgtcttt ctcataatac tcggcctctt ccccaccact
     1201 aaatggatct tcaatttttg gaactaacgt aattgttcca tcatcagaat acacaacgat
     1261 atattcttgg ttttctgaag gtttctttcc gttatcagaa ggtaaagtta ccactacaga
     1321 acttccttgt aatctagatt ttgtaacaag cattatcatc actcccttaa taaaagtata
     1381 ctatataaca ctaagtaacg caaaattccc ctttgtaaac tatctagaaa attgttttgt
     1441 ttaaagtata tcttattgga tataaataga aataaattgt agccagttca ttttttcttt
     1501 aactaaaaaa ataatccaag aaacattgaa cctatggatc caaaagctac ccaaatacta
     1561 tagctaaccc cccgctacct tcaattttgc agataaaacg ctttgggaga aaatttcaaa
     1621 gacaacaggc catcctatca tgcccgatta taaacaaaaa gctggctatt ccttttgctc
     1681 tgacaaattt caattgggaa agttcacgaa actagcaggt gaagaagttt caactgttcg
     1741 gatcaaagaa tgcccgcttc aaatagaaac attggttcaa cgaatttcaa ctagacaaga
     1801 ctttgctatt gtagaacgtg agatcgtagc tatttttgta gaagaaggga ttttacatga
     1861 tccaactcat atcaatgtgg ataaatggca acctcttatc tataaattca gagaatatac
     1921 aactgccaac cagtcgttaa ctttgaattt tagattccaa gaattcaaag agtcataatg
     1981 ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac gagtaaaaaa caaatagaag
     2041 ctatacatta gaaaggtcga ctgtgaccct atagatgcag tatatgcagc ttatgagcat
     2101 ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc tttttcgttt tttgccgctc
     2161 tttctttgaa aaatcgatta tgttatacct tctccattac taattcaggc atctgctgag
     2221 taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg ttcgatttgt tcttcagtca
     2281 agacaagcgg cattcggtca tggattggtg cgactgtgcg gttaggttca gtagtcatta
     2341 tgattgattc tgccattttt tcatcctgat ggctacgata aaaaccgcca atatacacta
     2401 ttttttcctt atttgaaaac ataaatttgt ctttttcttc attccactca ttgggaaaac
     2461 acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt tcttctactg tctctgcacg
     2521 ggcattaatc atcaattgtc cttttttaaa tccggtaaat ccccacttca tgatttccgg
     2581 agtgaggatg ttttcttcct cttttcttaa cgtcacaaca agatttgacg gaaaaacttc
     2641 tccagctgct aattctgctg cctgcttcgg ttgtttcgtc tgggcttctc gataataatt
     2701 ctgaaggagc tctgactcta gatcaaaaaa ataacgacca cacatcctaa tccctcccaa
     2761 tctttttttc tatcatacac tgcaacaaga agttcgcacc aacaaaacgc tttcaatttt
     2821 tgtagaacta aaaaagttgt gacaagtgac aaactctcca gtgaatggta ttctcctgcc
     2881 aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgcttt ttgaaattgt
     2941 tccttgtgac tcggagtgaa acggcaggct tacgacctct gggtaaacgg tgttcaaaag
     3001 ccagtttctc ggtattaagc caaatttatg caaaaatgta gaaagctatt ttcacaaaat
     3061 ttttcttaat actcgaaact aagcgacttt ttcacaacct cttccttaaa cggtgttcaa
     3121 aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tatgagcagc tatttttatt
     3181 tggctgttct tattacttga tgcagggcag ctttttcaca acctctccac taaacggtgt
     3241 tcaatcagct aaccttcggt attagctcaa aaaacggcaa aatatgagga actattttgc
     3301 cgtttgtttc tctaatactc aggtcaaagc gctgatttca caacctctaa tacacggtgt
     3361 tccagaagta gcttcttcga aaaataagcc gaaatttcac aaaaatttga gaagcaattt
     3421 tcgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc taattattaa
     3481 agaacctttt gcgaagttaa caatctttct ttgatcaatt gaaaattctg cctaagtagt
     3541 gatagatcct gtgtgacttt caacaataag atacttccct ggatttcagc aatcaacgct
     3601 ttggcttgac gatctgcttc ttcatcggac agtttttctt cagaaagtgc caggatattt
     3661 tgtttcgtat acttgaccca ttgattgact aattctagta gcaacagacg gtattcttca
     3721 tccactgtac tcaactcaat aatcaaatta cctacaggac agccatgata agtgatctgt
     3781 gtctcatgat cagcgaaaat ccagtccaac atttcttcta atgcttcttc tggctgtttt
     3841 ccagcattta agatgtcttc gaacaattct gtctgccata cgtccatatg cgcatggatc
     3901 accgctagac aaacttcttt ttttgaagaa aagtaataat aaaattgacc ttttcctgta
     3961 tcagaagctg ctagaatatc tcggattgac gtttcatgat agcctttttt ataaataaga
     4021 tctttggtcg cttccaagat cttttccttt gttttcaatg atattctcct ttttcatttc
     4081 tcatagtcct atattataca gtaaattcat cattttttct tcttaatcgt ttaaaaaaga
     4141 ctgtgccaaa ttatctgaca cagccctcac tataaaactg ttctcatata gctagatgtt
     4201 cattgattca agaactgcga ggacaaaagc aattcctatc ttatgatagt tgctctaata
     4261 cttcctctgt tgtcatcact tgatctgcaa taaagccaaa gttcgtcaac gctgcatcca
     4321 ttgcttcttg gccaggagca ccaattgcat ctgtcgctac cacaacattg aatccactct
     4381 ctaccaattc acgcatatgg gaatcgctac acaagtttcc gttcattcca ccaagaatta
     4441 ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc attgctttct ggtccaaaga
     4501 ttttatgagg agagctgatg atcgtatttt catccaaata aggtttcaat tcttcgataa
     4561 aatcggctcc tgaaccttct tccactgatt catattgact ttttctagca aacatctgct
     4621 gctcaagcat catatgttct cctgcaccgc caaattgcca tttatggtca tgtgggtaaa
     4681 aataatgagg agaaataaag agtttgtatc ctttttcttt tgcttgtttt accatttcaa
     4741 tcatattttc gatcgtattg tatttttcta agatatcttt tgtcaaacca aagccaccgc
     4801 ctgctggttt caagaactcg acttgcgggt cagtgagaac tactgctgta tttttattat
     4861 tgaacatggt ggattctcca ttcgttattt attttaatac atcatttaga gcttcactca
     4921 ttgtcggatg agaatagatt tgatctctca ataacgtata aggaagtttt ccattcatag
     4981 ctagcgaaat caagttgatg acttcatgag aatcttccgc atagatactt gcccctagaa
     5041 tcaagtcagt ttcagggtcg actaaaactt tgaatacacc tttaggatct tctaatactt
     5101 gtgctttagg gacaccgctt gtcatatact tgaataattt gtaattgacg ccttgtgctt
     5161 tggcttgttt ttctgttaaa cctacattgg ataaagtcgg agtgataaat acactgtaag
     5221 gcacagcttt ccggttattc gttgttcgtt tattctcgcc cttcaactga tccataatga
     5281 ttcgataatc atctagagag atataagtga actgcaatcc gcctttgacg tctccaatcg
     5341 cccaaatgtt gtctgcatct gtacgaaggt attcatccac agcgattgct ccgcgatctg
     5401 ttagtttaac atctgtattt tctaatccaa gtgatgctgt acttggtttt cttccagttg
     5461 cagccaaaac ttttgacgct tggattgtta cttcttggcc atcttctgta aaggtgacgg
     5521 cagctgttga ttcttggtct gctacttgat ctacagatac acctagatgg aattcgattc
     5581 ccgcatttgt catgtcttct aaaatcattt gcgcgatatc ttcatcttca cgtggaagaa
     5641 attcagaatg ggcatcaaga acgacaactt tagatccgta ttcattgaac attgatgcaa
     5701 attccaagcc aatatatcca gccccgatga tcactaattt ttccggtaat tgttcctgat
     5761 ccatcgcttg cgtagaatca atcaagtagc gacttgtatt caatcctttg attggtaaaa
     5821 tgattggttc agaaccagta ttgatgaaaa tccgttcgcc ttcaacaatc actttttcac
     5881 cattattcgt cacttcgatt tgatggttag aaagaaaacg tgctgtaccg tccatcactg
     5941 tcccagttgc ctcatcgctg atcatatgat aatttttggc gcgaagcatg cccgtcaatt
     6001 tttctttccg agcaacagcc tctgtaaagt cgattcctct ttctccattg aaaatcaatg
     6061 atttagaagg aatacagccg atgttgatac atgttccgcc atacatacgt gttgactttt
     6121 caacgactaa aaccgattca ccttttgaag ctaacgcctt cgctaaagtc ttaccgccct
     6181 ttccaaaacc gatcacaata ttttgatatt tttccatcat ttttccctcg cttctttttt
     6241 acctaccgat tggtacaaaa atatgataac cgataattgt ctattctcca aatcatctgc
     6301 tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc acgtttttag tctggttcga
     6361 tttcctttat tcttctaaac aatcgttact tctacatcga tatttcctct agttgctttg
     6421 gagtaagggc agaattcatg cgcatcttct gcaatttttt gggcttcttc ttcgctgatg
     6481 ccatcgatat gcacttctaa tactacaccg atctgatagc cgccatcatc taaattataa
     6541 agagagacac gtgcttttac tgtggactct gctgtcattc ctgcttcttt gatcatatgt
     6601 tgtaccgccc cattgaaaca agaagcataa cctgctgcaa ataattgttc tggatttgtt
     6661 ccttctgcat ggattcctgg aggagtgatt ttcatatgca tcgagccatt tggtgcctcg
     6721 acttctccat tgcgcccgcc atggttgatc attgctgttt cgtatgcttt tttcattgta
     6781 ctgctccttt cgattgacca tttgctactt ggctataatt atagtgtatg acaagtaagg
     6841 cgctttctac aaaaattctg cttattcgaa taccagaatg ccgtcttcta ctatttttcc
     6901 aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata tttgttccaa aataaatagc
     6961 aatcttttct acataatctt ttatatgatt gattttaaga tacatatata ccagaaagtt
     7021 gaccccataa accaatactg ttaaaaaaat aaccatattc gttaccattt tttcctcctt
     7081 ttgctggtct ccctaccagc actcgattta accataccac tttttttgcc gtccaagcaa
     7141 ctagcctttc aaaaagactg gtttattctg ctgttaatga ttcaagatca gaaaaatgtc
     7201 cgaataattt tgctaatttt actaacgcca acttgttctt tccgaattca tcattttcta
     7261 tctgtaatac tagtaacggc tcatgtaagc ttaaacggat caaaaaccag ccgtttccgt
     7321 atggctcttc tagatcatat cgaatacctt cttcattttc cgggtcgaag ttcatccctt
     7381 tttcttcttc caacagtcgc ttcatttctt ctaatatctt gtttccttgt tcgcgtggtt
     7441 catcgctgaa caattttaat cggatttctt gtacttctac gggctgtttc aactctttga
     7501 tttgttcatc taatgtgata ccttttcttt ttaattctgg caaaagcatc aggatctttg
     7561 cgatgacata tgcaccatca tccagaaaat agttttcctt gaatgcagcg tgtccgcttg
     7621 tttcgatggc tagctgtgta tctttcccaa gacggttaag ttctaatgct ttattgatta
     7681 catttcgata gccggaaata taacgaactt ggatgcctcc tttttcttca atgaatcttt
     7741 gtagatgaga agaagtaggg gaattcgtca cgattcttgt tcctggatgc tcttttaata
     7801 cgattgtact taatactgcg atcaaattat ttcgattgat cactgttcca gaagcactga
     7861 caactgcaga acgatccaca tctgtatcga aaatgatccc aagatctgct tcatttgcta
     7921 aaacagcctg cttgatactt tccatcgctt ctttattatc aggatttggg atatgattag
     7981 gaaatcggcc atctggttcc aaaaattggg aacctgttga atccgcacct aagctattca
     8041 atactttttc agcaaagaat ccaccagcac catttccggc atcaacaatg attttccaac
     8101 catttagtgg tttgctttct agtgttccaa tccccttttt aatttttgtg actaaatctt
     8161 gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc agtttgttcg ctttttattt
     8221 tttgctgctg tgataggata aaagcaatat cttctttttc tgctcctcct tctgaggtaa
     8281 agaattttat tccattataa taatagggaa gatggctagc agtcagcata accgcagcat
     8341 cgcacgagaa ctgttcaaat tgagtggaca taaacatagc aggcgtagta gctaattcga
     8401 aatcgatgac atgtatccct tgttctttca gactttggat caacgcctct ttcagtgctg
     8461 gtccgcttat cctactgtca tgaccaatcc caatcgtcca ttttttattt ggatcggttg
     8521 atttcttctt tttctttatc cagtttgcta gtccccagcc gatctttttt accatgtctg
     8581 tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat tcctcgaata tccgatccat
     8641 tttgcagcgc tttcatatct ttcatataaa agtcctcctc atttattccc tcattttttc
     8701 cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg caaataataa tgaatgtatc
     8761 tacaccggcc atcgttccga caacttcagg gtaagccact tcatctagcc aattcgttac
     8821 cacatcggca ccatttcgtt cagtcaatac gataaccatg aactgaacag actggatacg
     8881 caaaacctcg cgtctaattg ctgtacgcaa acgttcttca tttaaagaaa cagatggctg
     8941 gttaaataat gtatagcgta ctattttgtt ttcattttga ctttttacta acttcatttc
     9001 tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg cctgcttcat gcagccgcgc
     9061 aagcagctct tcctgtgtcc caataggttg ttctgtaatg atctggcgga tcagtgcttg
     9121 tcgctgtttc tttttcatat tagacacctc tcaatgttga ttatagcaaa agataagtaa
     9181 tatttcttca tattttggta ttttttactt ttattttttg ttttcattca ggttgctaac
     9241 ctttttctat tttaacacgc aaaatcgtct tcattttttc aggtgctgat tttcttggat
     9301 cgctgatgta aatctctttg tgttctttag aagctctttc atagccttct tttgcgatga
     9361 atgcttctaa tttctcaaac gttgctggtt cttcggcata gggcccgacg tgtaagattt
     9421 gggcacaaag accttcttcc atctttttga atactacttg ttctttcaag tcttccggaa
     9481 gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc tgtaacaaag tcaggctgtc
     9541 ggatcatcaa ttgataagaa aaatgttctt ttttcatcac tggttcatgc aaatactttt
     9601 cttgcagtcc ccattgtcct tccagtggat agacagtata catctgataa tcaggaatca
     9661 gccaattttt tttaggcgac atgcgtattg catagctgac tgcatataga caggctactc
     9721 ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct gatgctaaag aatgattttt
     9781 taggaacctc tagtatttgc gggctttttt tcatactata gctcttgtct ttacgccatt
     9841 caattttttc catttttctt cctccgcgaa attccattta gttatttatt atcatacctc
     9901 tttttgagtg acttggctaa tcgtgttgac actatttttc agtttgtgct agaataagtg
     9961 aagtaaataa atcgtacaga taacacgttt cggcgtgtcg agagagaggc ggtttctatt
    10021 tttataatag aaatcactct ctttttttac gaaatttttt agaggtgatg taaatgacaa
    10081 aacaaaaaca gctgctttta gtacctgcag taattgcaac agggatgatg tcttttgcgg
    10141 gcgtattgat cgagacggcc atgaatgtaa ctttcccaac tcttatcaga cagtttgatt
    10201 taacgactgc acaagtccaa tgggtaacaa cgatttatct tttgatgatt tcgatcattg
    10261 ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa aaaactcttt ttagcttcaa
    10321 atctgctttt cttggcaggt ataatgatcg atttcttctc tcctaccttc atggtcctat
    10381 tgtttggtcg attgctccaa ggtgcagcga caggtatcgc tcttccgttg atgttccata
    10441 ttatcttgac atttgccccc ctcaatcgac gaggcgcgat gatgggtgtc ggtactttga
    10501 caacggccat tgctccagca attggcccca cttatggagg cattatgact tcacatttta
    10561 catggaatca tatcttcctg tttcttgtgc ccgttttgtt tctttctttg attatcggct
    10621 ggtttgctat tcccgagatg gcagtacaaa aaagtggtta tttggattgg ataagcgttt
    10681 taggaatttg tcttttgttt agtggaagtc taacattttt gagtacgttg ggaacagttt
    10741 ccagctggat tgctttaatc atcggcggta ttggctggtt ccttttttac tatcgaagta
    10801 aaaaagtgga gcatccactg atccgactgt ctattctttc aaataaaaca ttccgcttgt
    10861 ttttgattgg cttcttagtt tatcagtttt tattacttgg tgtatctttt gtcttgccga
    10921 atttccttca gatcgttcaa ggaagtagtc cttttgtcgc tggattagcg atgcttcccg
    10981 gcgcagcggt tggtgctttg ctttctcctt tttccgggaa aatgctggat caatatggtc
    11041 cgaagaagcc aatttttgct gggctgattc tttctttgat tggttggcta gcattattcc
    11101 tactcatagg acacgcttca gtgggtgtat tgattgcttg tcatgtgttt tatatgatcg
    11161 gtatcggatt atcttacagc aatatgatga ccaccggaat gaatgcactg aatgaagaac
    11221 tacaaggaga cgggaacgca atgttcaata ccttgcagca gttttctggc gctgtggcta
    11281 cttcattagt agcggtcatt atcaattacg tccagcatca tacttcccat aactatgaag
    11341 tgtctactac attaggatca aaagcagcat taggcgtctt gttgctgttg cttctcgtaa
    11401 gctttgctcg attcgcctat tatctgttct ttgctaaaaa agcgtagatt ccatatacgg
    11461 caggtgttgc atctgccgtt tttttgtgtg aaaatggttg tttgtaaaag gaggcttttt
    11521 tatggaaaaa attgccatta tcggtggcgg gatcattggt atgaccctag ccagttactt
    11581 agatacatca aaattcaatg ttgctttatt tgatgaaggg acaggacagg caactaaagc
    11641 tagcgctggg attatctccc cttggttgtc gaaacgcaga aacaaacaat ggtatcgtct
    11701 ggctaaagac ggcgctgctt ttttccctaa acttataaaa gatttccaac ttgatcaaac
    11761 gatttacgca caatcaggga cacttttgct tcgacctgaa aaagatttag ctgaattggc
    11821 tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt ggaaacatcg aatggctttc
    11881 acctgaacag acatctcaag cacttccgtt attgaaacca gcagtttcac tgcgtatcag
    11941 tggtggtggt cgtctggatg gaaaagcata tttagaacat cttggtcagc gtttagcaca
    12001 aaaacaaatg gagcagatcc aggcaaaagt cactttgcat aagaagagcg attcttggct
    12061 catcaaggga ccgtttggag aatacacagc tgatcgtgta attttgacac ctggcccggc
    12121 acttaagcct ttattacaag aattaggcta tgctgcagat atacgtccgc aaaaagggca
    12181 gctcttagca tttcagacat cttataagat gagtcaagac tggcctgttg ctatgcttga
    12241 cggagaagct gatttcattc ctttcatgga tggaaagatc cttttaggtg ctacccatga
    12301 aaacgatcaa ggctgggatt tggctcctac tagcgaagct tataagggat tgactaagag
    12361 tacagaacag ttcttgactg atccagatca gataaaaaac tggccatacg agttccgtgt
    12421 aggtacccgt gcttatacgt cagatttttc cccatttttc ggcgtactgc cagatgatcc
    12481 aagcttactg gctgctagcg gtctaggctc ttctggcttg accacaggtc cttatatcgg
    12541 ctatctatta gccaacttct taaatactgg tcattggcct acggaagatt atcaaaagga
    12601 catctccacc tatattacga aaaatccatc atagaaaaac agctttttca agtaaggatc
    12661 ccgagcgata ttgcgcatgg attacttctt gaaaaagctg ttattttagt tcaatttctg
    12721 aatcaaattc tccaaacttt cttttgcttc atctggcaag agatcttttc ctatctgagc
    12781 ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc agatttttat aggacgtatc
    12841 ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg atgtcttcaa atggaccaaa
    12901 cgacttgaat tctgccgttt gttgcacgat tctttcaacg attgacaatg tcgtttcagg
    12961 tttgattttt ttatcatcga aatatccagt attcaggaca tagcaggctg tttgctgttt
    13021 caaaaacaac tctcgaaaag cttgatagtc ttccctaaga ggatagacac ggaaaggatt
    13081 agcaaatggt tcaatgacta gctcatctag ctgtcttttt gtagtgatat tttccgcgtc
    13141 tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct aaaactgggt cattgatctt
    13201 gataattggc ggaagcgaat catctttcat gatccaaaaa acagcatcga tcttttcgct
    13261 aagatgatcg actcggtcgg gtgtcgcata acgtgatttg actgtccgcc catttccgtt
    13321 tcgtatatct cctgttacta ggactttttc tcctttactt gtacatgtca cgcccacatt
    13381 ctgacaagta aggaaagaag aaatggccgg atcatcagac ggatagtctt gtgttttatc
    13441 aaagtaagaa ggttccaagg cagtagcaaa accatttttt cgagaaataa cgaaagcatc
    13501 gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt gctaaggtaa tcgttgattt
    13561 tccagaaccg gacaacccaa atacagccat cgtataggtc tggtcattac tagcatattg
    13621 tttcatccca ccgtggcagg caataaagcc attgcgattt cccaatgccc aagcaagcgt
    13681 caaagtcgct ttttttaatt ctccaaagta acgtaatcct agcacagccg ctgtattatg
    13741 gagagcatca aacaaaatga gtccatttgg aaatgtttca tgtgtccatt cgggatcaca
    13801 aaagatataa agatctccct catcaaagga aacagacttt tgataagtct ctaagatctt
    13861 cgaattcgct ggctgaaaat tcagcaaata agaatacaaa ttgttctcaa atccttctgg
    13921 aatcagcaaa tgactttgca cagaaaattc ttcagatagg ccaacgatta cttttgtatg
    13981 gtaaaactgg cgcttgcgac tttgatacac agcttctctt aaaattttct cgtatttgct
    14041 ttgttccttg tctaagtcct caaagatccg ccgagcactt gctgttcttc ccacaatgct
    14101 tccatcattg aatactagtg tcctaacatc agctggtaaa tctagttctt cagcatgtga
    14161 gattgttaaa tctgtgatga tcgttcctgg actccctttt gctagttgat aagctgtctt
    14221 cctatcaaaa acttcatgaa cattattacc gtaaaatgtc gtttctacaa tcgatcgaat
    14281 actggataac agtggatttc cacttgtgat ttcgtttaat tcgagactct cttttgtact
    14341 catggctcct cctcattccc taaacaaaaa aacgttttct ttattataca ttattcgagt
    14401 ttttatttgt actgctgcgc tgtgggaagc aaacgagtta tttttcacac aaaaaaaccg
    14461 agaacttgaa taaacaagtt tctcggtttc atcattttcc ttttatcggc ttagtaccag
    14521 ccgtttgcta accagaatgt tttagcagca tcccatgaac cataacgaga tgttacatat
    14581 tgttctgcta cacgttcttg attggcagct gaatagtcgc cgtttaagta tgaagcatct
    14641 aattgataac ggccgatgta acgtccgtta gtagctgtgt atgaaccgcc tgattctttt
    14701 tgtgcgatcc attcttttgc agagcttgaa gttggcgctg ttgcagcagc tggggcagca
    14761 ggagctactt caactgtttc aacaggtgca acgtatgtat gttcttgcgt tgctgtttca
    14821 gttgtagatg ctgtattttc tgttgttttg ttttctgttg ttgctgaaga atcatttgaa
    14881 gttgggattg ttaattgttc accaacataa atgcggttga tgtttgcgat cgaatttttt
    14941 tcagcaattg catcgatcat tgagttgtct cctgcaaatt tttgtgagat cttagataat
    15001 gaatcacctg attttactgt ataaacttca tcagcgtgag catttgtagt tcctacaaac
    15061 attgcagtag caccagcagc aaaagttgta cctaaaacaa ttttcttaaa tgaattcata
    15121 ttaaactcct ttttttctcc gttttttatt ctttcgacgc tattaacttt atcatttaga
    15181 aatatttcat aggtaaaaga aacatgacat ttaatgacaa aataacgaat tatttaatat
    15241 ttgtaacatt taaacaaata tttttttgat acaaaaccat aaaagccttg ttgtataagc
    15301 aaagtaatcg tttttctatg tttttttatt tttgaatgaa aaacgcagat tctgtaacat
    15361 tcactgcaat aatccataaa aaaaagcaaa aaaatttttg atttttatca aattctctta
    15421 aaaaaatgta aaaaatacaa aaaaaccagt aattgttaca acaattactg gacaaaaact
    15481 ttgaataatg ttaactattt ttttatttta actttctccg caataatttc tgggcatgtt
    15541 ccaattcgtt tataacgact tcttctgttt ctttagctaa tatttcatct aatacatcaa
    15601 gccatttttc cggttctttt gttcctagac gtccaatagc ccaagctgct gttcctctga
    15661 tgacagggcg gacatctgtt aaacattctg aaatctcagg caatgccgtt cgatcaccta
    15721 gattagctaa agcaatcaat gcgtttctct gcaaaggttt cttcccacgc catgaaccag
    15781 ctaaatgccc aaattgctgt ttgaagtctt tattagacat cgaaagcatt ggctttaatt
    15841 ttggatatat ttcgtctact tttggttcca tctcttcatg aaaatgaaaa tcttttcctt
    15901 gattgtaagg gcagactaac tggcagatat cacatccata aatgacattt cgcatttttt
    15961 tacgatattc ttccggcatc atcccttttg tctgagtttg ataagacaaa catcgctggg
    16021 cattcattct tccgtcacca agcaatgctt ttgtcggaca agcagtcacg cagcgcatac
    16081 agtcaccgca accaaattct cctggtttat caggctcaaa tacaatatta gtaacgatct
    16141 caccaagata gacaaatgaa ccgaactctt cagtgatcaa caatccgttt cgtccaataa
    16201 atcctaaacc agctcgctgt gctacagcga catcgatcag ttctccagta tctacttgcg
    16261 gagctagacg ccattcctct tctttttgcc atttttctgc tcttgtttgg atgaattcga
    16321 tcaatcggtt caaacggtct ctgagtatat catggtaatc gatcccccaa gaagcacgag
    16381 caaactgccc gcgtttttca tccctaggca cttcttcatg gatcctagta ggatacgcaa
    16441 gtgcaatgga aatgatcgat ttcggctgat caaatgtcat ttctggataa agacgctcat
    16501 cgatatttgg atgttcaaag ccagaagtat ggccagctgc tttttgttct tccaacgatt
    16561 cttttaatga atcaaaaggt tccgccgtgg tgaacccgat tttgtcaatt cctaatcgtt
    16621 tgctttcttg tatgatttct tcttttaatg tcatggtctt cctcgcttgc tgttaacggc
    16681 tgtataaccc ataatatagt ttttcttttt tcagctggac aaactggatc tgctgatcgg
    16741 caatcttctt ttcttccatc catttttttc ttgcagcaaa tacttcccaa taatttgttg
    16801 aaattaattt tcttttgtta tctaatgcat taaaaaaatt caatgaaact ggttttgtac
    16861 ttaacagttc atggagcctt acctcttccc atggcgaaaa ctcagacaga tcgcgggaat
    16921 acaatacttc ttcatagaga tttttttgct gtgtgtacgt gaatgtttgc gggattttcc
    16981 caggcctagc aatttctact agacgattat aaaacatatt cattaatttg ctgttggcaa
    17041 taaattgact tttataatag gccgactgga tctttttgat tactccttct gctttgatga
    17101 ctgaaaataa tgcaggccat gctttgacgg ttatttcgtc gttttgttta actaatacgc
    17161 caggtgtgaa gccgactgga gaaaggaaca caaaataacc gataccggta ttttcatcta
    17221 aaggctgaaa ttcatcaaac aaccatttgc ctatgtacag ctcttcatct ggttctgttg
    17281 tctcattttg atttgctatg aataatttag ctaattctgc ttcaagaaat tgaaggtctt
    17341 gatccttgtc ggtattttcc tgctggctga tcaatcgaaa gacttcttct ttaatcgtat
    17401 atttatcttt acgtgtaaaa ttcatagcca caccttcctt ccttactcta actataacgg
    17461 aatttcttca aaaaaggtat ctgtacgacg aaaaacagca taattttttt tcatatctct
    17521 tataaaaaat gcaaggtctg ctcctcaagc tagaccttgc acttttcagc tttcttttgt
    17581 ttgtgacaaa ccttcatgga tcgtttcaat attccatttc atcatctgat agtaagaatc
    17641 tccttgttct ccttcttttg ctatggaatc agtgaacagt ttatcataga ttttcaaacc
    17701 cgtttctttt gctacccgtt ccatgcttct tgagtctaca ctggtttcca cgaataaaac
    17761 cggtacttct tttgctctta tctgatcaat gatcgctttc atttgatcag gcgtaccttg
    17821 actttctgta ttgatctccc atatataagc tgctggcaaa tcatatgctc gtgaaaaata
    17881 cttgaatgcc ccttcacttg ttactaataa ttttttgttc tcttcgatag aagcaaaact
    17941 ttccttcgct tgtgtatcta attcttttag tttttccaca tatgcttctg cattttttta
    18001 tagagtgtcg cattttctgc atctttttca gagaatatac gtgcgatttc ctccacatat
    18061 tttattccgt tagataggtc tagccaagca tggggatctg cttttgtatg ttcttcaccg
    18121 ctagttaaat aaagaggttc tacatttttg ctaactgcaa aataatcttt tccttcttct
    18181 tttttagccg tttccatcaa gttatcgaac cagctgttac ctgtttcaag attcaaaccg
    18241 ttgtataaaa taacatctgc atcgcttgcc tttttgatgt cttctggtag tacttcatat
    18301 tcatgcggat ctgttccgac tggtacgata ctgtggatat ctatttgatc tgttccgacc
    18361 tcttttgcca tgtccgccaa gatcgaattg gtagccacga ctgacaactt ttctttactt
    18421 ccttcatccg cttggtcaga agcctgcttt ccgcaaccgg ctaataccaa gaaactaact
    18481 gccaagatta aaaagagtga tttttttgtt ttcatctatc tgcttccttt ctgaaataaa
    18541 attccttttg ttggagaaaa taaaaaagca aggataaaaa atgcggtaca tgctaacacg
    18601 atcgtagctc ctgatggcca attgtaacta taactgaaaa acacaccgac aactgcactt
    18661 agcataccga ttcctgcagc tgtaatgatc attttcagta aatggttcgt tagcaagtaa
    18721 gccgtggcgg ctggtgtgat caacatggca atcaccaaga ttgttcctac tgtctgtaaa
    18781 ctgactacag ccactaatgt caaaaagaac atcaaaagat aatggatcag cgatgtgttc
    18841 agcccataag cttttgccat tgtcggatcg aatgatgtga tcttcaattc tttataaaag
    18901 aaaaagacaa atatcaagac gattccacta acgagtgctg tcagcaacaa atcgctttcc
    18961 ctaacagcta agacattccc aaataaaata tgatacaaat ctgtcgcact ctgggcatat
    19021 gaaatcaaga taatccctaa tgcaaaaaag gagctaaaga caattccaat cgctgtatca
    19081 tttttcaatc ggctccgctg agtcacgaaa ccaatcactg ctgctgttag catcccaaaa
    19141 gcagtggctc caaagatgta actaaaacca aacatataag aaatggctac acctggaaga
    19201 actgcgtgag agatagcgtc tcccatcaaa gacatccctc gtaaaataat aaaagaaccg
    19261 atgactccag aagcaagtcc tacaagcatg gaagtgacca aagcgtattg caggaattga
    19321 taattcatta atccatcaat aaaggattgg atcatttttt ctctcctcct tttactaaat
    19381 gtctgatgat ctcaccatag gccaattgaa ggttcttcga tgtaaagact tcttgtactg
    19441 gaccggaagc gatcagctgt ttatttaaaa gaataacctc atcgaaatat tcttctactt
    19501 tatgaagatc atgatggacg atcaaaatcg tttttcctga attcttcaat tcctgcaaga
    19561 tgtcaaagat ctttcgttca cttaacgcat caatccctac gaatggttca tctaaaaaga
    19621 tccattctgc tccttgggct agagctcttg caataaaaac tctctgaagc tgtccacccg
    19681 atagttcgct gatctgtctt ttggcatatt cttccatccc tacttttttc aaagcttgtc
    19741 ttgcacgttc tttttctttt ttcccaggtc tttgtccaat tcgtaaagat ggatatgttc
    19801 ctaaaagtac aacgcctaat acatcaattg gaaaggaaag atccaattca cttcgctgtt
    19861 ccacgtaggc tattttttta cggactgagt ttaccgactg tccagaaaag gtgactttac
    19921 cagtagtagc tggtatcaac tctagcaaag atttcataaa tgttgatttt cctgcaccgt
    19981 ttggtccgat aataccggta attttttgtg gatttatcgt caagttgata gttttcaaaa
    20041 cagttttccc ttgataagcg gctgaaaggt cttgaattac aatggccgta cgttccaaat
    20101 cttacactcc tttttaggtt tacctaactt tttatttaaa tatagtttaa ctaaaatgga
    20161 aagcattttc aagtagaatg atgaaaaagt ttttttcgaa ttttcctcgc attttatgaa
    20221 aattttctgt agaatcacat caagagttca ataaggctaa cctaataatg aaaaacatgt
    20281 gttgaaacgt ttgattattt tttcttgttg actcaacaac gaaaggagtt tttgtatgga
    20341 agaagaaaag ttaactcgaa gtcttagtgc ccgtcatatc caaatgatcg cactaggtgg
    20401 aacgatcggt gtcggtctgt ttatgggtgc ctcctcaacc atacgttgga cagggccctc
    20461 agtgatggga gcctatgcga ttgcaggatt ctttttgtat ttgattatgc gtgctttagg
    20521 cgaaatgctt tacgttgatc cttcgactgg ttcttttgca aattacgcga gcgagtacat
    20581 ccatcctgtg gctggctact tgaccgcttg gagtaatatt tttcaatata ttgtcgtagg
    20641 aataagtgaa gtgattgctg taggatctta tatgaattat tggtggcctg atttgccggc
    20701 aattattccc ggaatcattg tcgtcttgtt tcttatgctg gcaaatctta tttctgtcaa
    20761 ggcgtttggc gaattagaat tttggttttc gatcatcaaa gtcataacga tcattttgat
    20821 gatcattgca ggtcttgggg taatcttatt tggatttggt aatcatggtc aggcagtagg
    20881 gatttcaaat ctttggaaaa atggtggatt cttcactgga ggcgtcaaag gatttttctt
    20941 tgcattatcg atcgttgttg cttcctatca aggaatcgaa ctgatcggaa tgactgctgg
    21001 tgaagcagag aatcctaaaa agacgatcat tgaagctgtc cagtccacta tcggtcgaat
    21061 cttgattttt tatattggtg ctatttttat cattgtgagt atctatccgt ggaatcaatt
    21121 gagtgagatt ggttcacctt ttgtacaaac tttttctaaa gtcggcatta catttgctgc
    21181 aggactgatt aattttgtcg tgattacagc ggcattatca ggttgcaatt cagggatttt
    21241 cagcgcaagt cgtatggtct atacacttgc cataaatgga aagatgtcta aaaaatttct
    21301 gaaactatct cgtcacggtg cgccttttta tcctgtcgta gcaatctcag gtggaatctt
    21361 aattggtctg atcttaaatt atcttctacc aatgttcatc aaccagtcag aaaatatttt
    21421 cgttttcgta tacagctcaa gtattttgcc tggtatggtt ccttgggtag tgattttgat
    21481 cagcgagatt cgcttcaaaa agaagcacgc agataaaatg catgatcatc cttttaaaat
    21541 gccactatct ccttatacga attatgtgac acttgctttt ttagctatcg tattgatttt
    21601 catgtttatc aatcctgaaa cgagaatctc attgattatt ggactcgttt tcttgcttta
    21661 catgacgatc cattactttg tacgcgaaaa aagaaccgga aaataaaaaa agaacgaagc
    21721 ttcaccttag tggaagcttc gttctttttt attgctttta tcaattcaat tttagcgtct
    21781 caccgggagt caggaaataa ttctccatgc tcatcccatt cagcgacaag atcgtttcta
    21841 ctggtacgcc agttcgttcg gctatttgat ttggaccttc tccttccaaa actacggttg
    21901 tcgcagcctg tgaagtcgcg ctgttggtat tagtttcaga ggaatctgtt gtgtcttgtg
    21961 ttgttcgagc ttgcatttca gaagcttcta ccgactctgc tgtctcagaa tcagaagatg
    22021 ttgtgtcttg tgtgcttgtt gactctgctt gaagagaggc gctagtctgc aaataaatat
    22081 cggaaatctt agcgacatga ttcgtataat tagccgataa ttgattgatc aaagtatcta
    22141 agtctacacc gtctttattt tctgctgcct gatacgctgc acgtaatttt ctgatccctt
    22201 cgtcggagat tgcttgaagc tcacgtgctt tttggttagc aactgctgat aaaccagcta
    22261 ttccctcttg atttccctga atttctgtct ggtattcttc aatcaatgca ggcgtctttt
    22321 cttcgatctt tttcgaataa tcatttacga ttgcttgaag ttgctctggc aaaccatttg
    22381 ctgttttttc ttcagaagtt tgtgtctctt ttgttttagt tgatgactca cttgtttgtt
    22441 tcgtctctgt tttctttgtc gttgtttccg ttatggtcgt acttttcgct tctgacgaat
    22501 tttttccatg aaaatataga cccgcaactc ctcctgctat gatcaaaaac acggcaactg
    22561 ctgccccaat catgatcatc cgcttacgag ctgcctgttt ttgctgtctt cttcgggtca
    22621 tccgaacatt ttctccccac tcattttcat catacatttt tttcaccacc aatttttatg
    22681 tataatagcg ttatcaaacg cttcatattc ttattgtagc aaataattgt cgctgtgcac
    22741 atttttatct ttgttattta taagaaaatt tcaccagttg agatgattcc aagattgtcc
    22801 gtcgatttct ccttgcagct gcattgttcg ttcgtctatc tcagccatca gtttctttat
    22861 ttcatataag agcgcacggt cttgatattc tgatttttcg cttaattcat ctaattgttg
    22921 gatcagccaa tcatttgtca taatgaagtt cctttcattt cttgcagtgt aaaggtcaag
    22981 tcttcgatct gctcttttgc ctcagtcagt gcttctacga tatatgcttg tttttcagat
    23041 tcttcttcct gcattttctg attttcctgt actctttgaa taaaccagtc aatccgctga
    23101 gtcagcgatg gtttgccttc caatgagaga tactgctgat aagcaattag cttctcccaa
    23161 cgtttttcct gggataaata cataaattgt gctaaaacga atggcggaag ataggtcata
    23221 tggaatttat ttgcgattcg ctgatacggc ctcaaaaatt cggaaatcgt atattcttct
    23281 tttcctccag cttggtattc tttttccgcc acacctgttg tcaatacgat tcctagttct
    23341 ttccctttta atacatgtgc tgctcgttcc aagacattat cttgccaaat ttttaaatga
    23401 gcaggagaac tataccagta aaacggaaac tggaaaatga tccgatcatg ctgggccaag
    23461 agctgtagtt cagtctctat atctattttt ccgtctggat aacttgattc caaatgatgc
    23521 caagtagctg gtaaaccagc agaagcttct ttgaaaaatt gctgatttaa agattcttcg
    23581 atcgttggat ggctaatgat gataagtgta cgcatccctt ttcctccttt ttagtcttcg
    23641 ctttacagtg tagcataacc gccacaagtt tcttttgccg atgcttgtca ttttaaccag
    23701 actaatcagg ttgtccaaga aactgcggtt tgtttttcca actgtttttt caaggtatcc
    23761 aaagcaatat tcttaaaatt ttcgtttttc atacgtttac gatagttcgt agccgtttct
    23821 ccggtactct ttttgaacat tcggctaaat acgactagac tattgaaccc tacttgatag
    23881 gcaatacttt ccacggattc atccgtcaga gaaagaagaa acgcccctcg attgatccgt
    23941 accccgatca aatattcctt gatactggta cccatatgat ttttgaatac tcgcgacaaa
    24001 tggctccgat tgaccgctaa atgctgggcg atttcctcaa cagacaattt ttcagcataa
    24061 tgtgcctcga tgtatttgac tgtttcaaca gctaaagagg aatgaacctt ttgtacacca
    24121 ccgctgacct ttccgccatc ttttaaaagg actgataaaa aacggtaggt taatgctgtt
    24181 aactgtaatt cattttccaa ggtttcttcc gtatctttca tgcattctaa aataatgtcg
    24241 atatattttt gtatgtcaga agagaccatc gtgtattggt cttctctgaa aagcgactgc
    24301 cgaatgattt cttctgcagc actcccacca aatgaaagcc agcaatacat ccacggatct
    24361 tctccatctg ataagtaaaa cgtcgagtct cctggacgaa tcaagaacag atcacctttt
    24421 ttcaattgga attgttgatt tttcacatga taagatcctt tgccttccat cacgacatgc
    24481 aggatgtaat cattgcgaat agcaggacca aaagaatgaa acggcatcgt tttagagaaa
    24541 ccacaaaaat caaaatacaa ttctttttct acttgttgat gttccttttt taaaaaaatc
    24601 gattgctcca cgtctatccc tccttactcc tttagtataa tgcaacaaaa agttaaataa
    24661 aagtcacaga aatatattaa aaaggaagcg atttcttgtt aagtttaagt cattaaaaga
    24721 tctttgttat tcgttgtaac cggtttactt agtattatat attgaacagg agaggaatac
    24781 agattatgaa aagcaaaatg atccaacgtg tgtcctacgc tttaggcgct tttggacacg
    24841 acacattcta tgttacatta agcacttatt ttatggtatt cgtgacaagc cagatgttcg
    24901 caggaacaga taaggcaacg aatgcaaaaa tgattgctac agttacaagc ttagttgtag
    24961 gtatccgctt agttgaaatt atttttgatc ctttgattgg tggcatcatc gataatactc
    25021 gtacaaaata cggtaaattc aaaccatggt tagttatcgg cggcgttgtt agttccgtac
    25081 tcttggtagc aatctttacc aatttctttg gcttagctac tcataatacg actttattta
    25141 tcatattatt tgcaatcgtt ttcatcttat tggatagttt ttattcattt aaggatatcg
    25201 ctttttggtc aatgattcca gctttgagta cggaaacaaa ggaacgtgaa aaatttgcta
    25261 cgtttgctcg tttcggctct tctctaggtg caaatggtac tactctagtc gttgtcccta
    25321 tcgttacttt ctttacctat cttgttactc ataaacatat tgaaggcgct tcaggctggt
    25381 tctggttcgg tgtgattgct gctgttattt caggcggtac tgcgattatc actgccttgg
    25441 gcacaaaaga agtcgatact gcgatccgtc agcaaaatga aaaagctggg atcaaagatg
    25501 tgttcaaagc agttgctcaa aatgatcagt tgatgtggct tgcccttgct tatatcgctt
    25561 atgcgattgc caatgcagca acaactgccg tattattcta cttcttcaaa tatgtacttg
    25621 gccaaccaac tgaattttgg atcgttggtc tagttgctgc cttacttggt ttagtagctg
    25681 ttccactatt cccaatgctt acacgcgtca tctctcgtcg ctacgtttac cttggaggga
    25741 tcgcaatgat gatttcttct tactttttct ttacgatcgc aggttcaagt ttgccaatcg
    25801 ttatcatcgg gctagtattg ttctacttcc ctcaacaatt gattttctta tccgctttga
    25861 tgacgatcac agactctgtt gaatatggtc aatggaaaaa tggtatccgt aacgaagctg
    25921 ttactctatc aattcgccct ttactagata aaatcgctgg tgctttgtct aacgggatcg
    25981 tagggtttgt cgctatcgct tctgggatga ctggtaatgc tactgctgct gatatcactg
    26041 ctcatggcgt aacgactttc aaagcttatg cattctatgc tcctgctgct ttgatgattt
    26101 tctccgctat tatttttgct tggaagatta cattgacaga gaaaaaacat gcacaaatcg
    26161 ttgagaaatt ggaaaacaaa ttggctccag ctgatacaat ggaagattaa cttcacgatg
    26221 cagtagttcc ttcccattaa gaaaggatct tgaaaatgaa gattacagaa aaagattttg
    26281 gccaaggtta tcacttgata accttggaga acaaaaacaa gctagcactt tctatctctg
    26341 atttaggtgc tagaatcgtc agtttaaaat caaatgatcg agaattagtt ttgggatttg
    26401 acaccgctga agaatatatc gaaaaagatc cgtatatcgg tgcttctatc ggtcgcaccg
    26461 cagggagaat cgaaaatggc cgcttttctc tcaatggaaa aacttaccaa ttagccacag
    26521 atccaaaaac tggacataat ctgcatggtg gtgctcctgg atttgaatta aaaaaatggt
    26581 cttatgtcat tttgaatggc gaaaatgaag cttctgtcat ttttactact acaagtcccg
    26641 acggagaaca tggttttcca ggtacaatgg atgtggaaat ccgctatact ttgacaaaag
    26701 ataatatctg gcgagtgacc tctcgcggca ccagtgacca agatacttta ttcaatccga
    26761 caaatcatgt ttacttcaac ttgacgggag atgcttctca gtcaatcgat caacatgagc
    26821 tttggctcaa tagcgaagct tatgctcctt tgcgaacaga tagtattcct ataggggtaa
    26881 aagaaaatgc tgcaggaagt gctttcgatt ttcaaatacc taaaaaatta gcgtccgttt
    26941 ttgctagtga tttggatcaa aaaaatcttg ttgacggaat cgatcatcca tttttcttaa
    27001 aagaaaccgg cttgggtaaa gaagccgctc gcttgactag ccccgataaa agaatccaag
    27061 tagatattgc tacagatgct tctagtgtgg ttattttcac tgcaaatttc ggcacagaaa
    27121 caccagaaat gcgcaaccga aaactggcac atcacggtgg catcacattt gagactcaaa
    27181 cagctccagg tgcagagcgc ttctcagcat ttggttcgat tcatttaaaa gccggatcag
    27241 tatttgaaac agtaacagaa ttcaaaatca aaacaagaaa ggaataactc atcatggaaa
    27301 tcaaaagaag cttaaacaca aaatttgccg aaacattcgg acctaaaaaa actacacaat
    27361 acttctcacc aggacgcatc aatttgattg gtgaacacac agattacaat ggaggccatg
    27421 tcttccccgc ttccatcact tatggaacgc aaggtgtagc agcacctcga gacgataata
    27481 aaatattagt ttattccaca aactttgacg atatcggtgt cattagtttt actttagatg
    27541 agctagaata tgataaaaaa gcaaactggg caaattacgt aaaaggaatg gtccttaaat
    27601 taaaagaagc tggacactca atcgatcatg ggtttgagtt attagtggaa ggaacaatcc
    27661 ctaatggtgc aggactttcc agtagcgctt cattagaatt attagtaggt gttgtattag
    27721 aagatctgtt tgatttaaaa acagatcgct tggaactagt ccaaacaggt aaaaaagtag
    27781 aaaatgaatt catcggtgtc aattcaggta tcatggatca gtttgccatc ggcttcggtg
    27841 agctagatca tgcaatctta ttagatacaa atacgctgaa atacgaaatg gttccagtga
    27901 aactagatgg ttatgcaatc gtcatcatga atacgaataa gcggcgggaa ttggcagatt
    27961 caaaatacaa tgaacgccgt agcgaatgcg aagaagcctt ggcacgtttg caaaaaactt
    28021 tgcctatctc ttctttagga gacttagatg aagaagaatt tgaatcaact attgaccaaa
    28081 tcggcgatga tacattgata cgcagagctc gccatgccgt ttctgaaaat cagcggactt
    28141 tgaaggcaaa agctgaatta gaagctggga acttagaagc atttggtcaa ctgttaaatg
    28201 attctcacca ctctcttcgc tatgattatg aagtaactgg gatcgaatta gatacactag
    28261 tagatgctgc tcaaaaacaa gaaggtgtct taggtgcacg aatgacagga gctggatttg
    28321 gtggctgtgc gattgctctt gtcaaagaag aaaatatacc tgccttcaaa aacaatgtct
    28381 atgatgaata catgaaagtt gtcggctatg ctccggaatt ttatgttgca cacattggaa
    28441 atgggacaac aaagatcgat ccagaagctg ttcatgtttg ataaaaaaat caacgatctc
    28501 agctaaaaac aaatagataa aaataacctc tcagcaattt ccaaatgtga gccttttgct
    28561 cgtatttgaa aattgctgtt ttttattgac acggaaagag aacgtgtgtt ctaattgtgt
    28621 tgaagaggtg attgttatgt gggcgaatca aaatccggta tttgtttatg aaaaagaacc
    28681 ttcaagagat attttatgta ttgactgcaa atctttttac gcctccgtag agtgcgttga
    28741 aaaggatctt gatcccctta caactaaatt agtagtcatg tcctatccgt ccgattcgac
    28801 agaaacacgg ggtagcggat taatcttggc ttccagtcct gcagcaaaaa aagcttacgg
    28861 catcaccaat atcagccgtg ccagagatct tcccttcccc tatccaagtg atttatatat
    28921 tgttgcccca aggatggcct attatatgga aaagaacaga gaaatcaatt ctatctataa
    28981 aaaatatgca gacgaagcga accatcatgt ctattccgtc gatgaaagct tccttgatgt
    29041 aacaaatagc atggcactct ttggtaaaac aaatgcgtac gacatggcta aactgatcca
    29101 taaagatgtg tttgagcaga ccggaatcta cacgactata ggaattggcg ataacccgct
    29161 acttgctaaa ctagccttag acaatggctc aaaagacgga aagcaaatgt ttgctgaatg
    29221 gcgttatgaa gatgtaccag aaaagttatg gaaaatctca gatatcactg acttttgggg
    29281 aatcggcaaa cgaacagcaa aacgtctgaa acaaatgggt atacattctg tttatgattt
    29341 agctcacgcg gattattatc acttgaaaag caagctaggg gtcatcggta ctcagcttta
    29401 tgcccatgct tggggcatcg atcgaagttt tttgggacaa gaatatacac caaaatcaaa
    29461 aagtatcgga aatagtcaaa tcctagatag agattataca agacgagaag aaatcgagat
    29521 cgttatcaaa gaaatggccg accaagtcgg cactcgcttg agacgatctg cagcaaaaac
    29581 acaagtagtc agcttatggg taggatactc tttaggctat cttgatccaa atggcaagac
    29641 cgggtttcac cagcaaatga aaatcgaagc gacaaatgct agcagagatc ttgcagatgc
    29701 tttattgacc attttccacc agcattacca ctaccaggat attcggcgtg taggcgtgaa
    29761 ctgttccgac ctcgtatacg cgacaggttt acagcttaac ctttttgaag atcctgaaaa
    29821 gcagataaat gaagcgaaag ttgattttgt tgtcgatacg attagaaaaa aatacggctt
    29881 tcaatcaatc atccatgcgc atagtctctt gcaaggagga cgcgccatcg cacgtagttc
    29941 tctcgttggc gggcatgcag gtggtatgag tggattagaa ggtggaaaag atgagacgga
    30001 cagcaaaaaa attcattgac tataacgagt atcgagatcg gccatttgga ttaaaatggg
    30061 ggactgcttt tgcgatggaa gaactagtca acggtatcaa aaaaatgaag aagaagcacg
    30121 caaacagcct cttcctaaac aacaaatgac attgcaagaa atcgatagta tactgacaga
    30181 agccttttta tctcgtaaag cagtaatcat tcagctcaat ttaaaagatg aatttggaag
    30241 actcttggat gaggtcgaag ggcacttcat tggtgaagcc tatgaagatt actttatgat
    30301 tgaccatcag ccgatattgt gggaagacgt acggtatatt gagttaaagg aaacaaaaaa
    30361 atggtttgtg acatgagagg ttgtgacaga agtgatcaac ttcaaaaaat aagaaaggat
    30421 ttttcgaaaa ttgcttctta aatttttgtg gaattttggc ttatttttcg aagaagcaac
    30481 ttctggaaca acgtatatta gatatcagag gttgtgaaaa agtcgcttag ttttgagtaa
    30541 caagccaagg cgctgatttc acaacctctg tctatctatc catctattcg gtaagattca
    30601 tgcatgacgt cttctttatt tagtatgtag agcccttttt cttctgacca ttcaacataa
    30661 atcagctgtt ttacatctgc atagccttct ttcttcaagt tctccataag ccatgcttca
    30721 gtcaattgat tggactgaag cactttatgc tgaatttgcc cttcatctac aagcaggatc
    30781 gataaagctt tgtcttcatc tgattttttc aatacactca tttgaccggc attctctagg
    30841 ataacatgtt tggcattttc aagaaaaaag cattcttgct gtcttaagat tgctcgcaac
    30901 tgctccatct cgatatggtt atttgtcaac tctgtcatgt tgatgacgcc gtctttgatt
    30961 agaacagatg gctcgccttt gacccaacga ttgacctttt catttttttg gacgatacgt
    31021 tcgataccat aaatcattac tgcccaaagc gcaatggcaa ataggacatg ccctacatgg
    31081 acattgtcat cataggttga ttcttctaag atcccaccta aaacaagcgt atagactaaa
    31141 tcaaacggtg tgatttctga catggatttc ttcccaagca aacgtaccac taataataaa
    31201 ccgatcaacc cgaacaccag tttcattgta attgaaaaaa tcattttttc tccccctaac
    31261 tatctgttaa ttaaattata tatgctttct cacgaacacg catggaatat acaaggaaga
    31321 aatgtatcct tttactaaaa aaaggactgt gacactgtag tgacccccaa aagttagact
    31381 tttttggagt ggagaaatcc actccttttt tttatgctct aaaatgatat ttttacacaa
    31441 ccgttacggt tgttttacga aattggacg
//
LOCUS       DAYYME010000020        29636 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000020 DAYYME010000000
VERSION     DAYYME010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.020"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..1016
                     /locus_tag="ACHBFW_000739"
     CDS             147..1016
                     /locus_tag="ACHBFW_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9317076.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="ACHBFW_000740"
     CDS             1225..2250
                     /locus_tag="ACHBFW_000740"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM9317077.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="ACHBFW_000741"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="ACHBFW_000741"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFM9317078.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3333..5036
                     /locus_tag="ACHBFW_000742"
     CDS             3333..5036
                     /locus_tag="ACHBFW_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9317079.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="ACHBFW_000743"
     CDS             5038..7071
                     /locus_tag="ACHBFW_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFM9317080.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="ACHBFW_000744"
     CDS             7082..7522
                     /locus_tag="ACHBFW_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9317081.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="ACHBFW_000745"
     CDS             complement(7722..8849)
                     /locus_tag="ACHBFW_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9317082.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="ACHBFW_000746"
     CDS             complement(8852..9529)
                     /locus_tag="ACHBFW_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9317083.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="ACHBFW_000747"
     CDS             9793..11559
                     /locus_tag="ACHBFW_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFM9317084.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="ACHBFW_000748"
     CDS             11574..12011
                     /locus_tag="ACHBFW_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9317085.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="ACHBFW_000749"
     CDS             12024..13175
                     /locus_tag="ACHBFW_000749"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFM9317086.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13237..13935)
                     /locus_tag="ACHBFW_000750"
     CDS             complement(13237..13935)
                     /locus_tag="ACHBFW_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFM9317087.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13928..15280)
                     /locus_tag="ACHBFW_000751"
     CDS             complement(13928..15280)
                     /locus_tag="ACHBFW_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFM9317088.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15503..16903)
                     /locus_tag="ACHBFW_000752"
     CDS             complement(15503..16903)
                     /locus_tag="ACHBFW_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM9317089.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17064..17801
                     /locus_tag="ACHBFW_000753"
     CDS             17064..17801
                     /locus_tag="ACHBFW_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFM9317090.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17926..18405
                     /locus_tag="ACHBFW_000754"
     CDS             17926..18405
                     /locus_tag="ACHBFW_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFM9317091.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18402..19169
                     /locus_tag="ACHBFW_000755"
     CDS             18402..19169
                     /locus_tag="ACHBFW_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFM9317092.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19399..19968
                     /gene="lepB"
                     /locus_tag="ACHBFW_000756"
     CDS             19399..19968
                     /gene="lepB"
                     /locus_tag="ACHBFW_000756"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM9317093.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20072..20674)
                     /locus_tag="ACHBFW_000757"
     CDS             complement(20072..20674)
                     /locus_tag="ACHBFW_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM9317094.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20780..21010
                     /locus_tag="ACHBFW_000758"
     CDS             20780..21010
                     /locus_tag="ACHBFW_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317095.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            21001..21249
                     /locus_tag="ACHBFW_000759"
     CDS             21001..21249
                     /locus_tag="ACHBFW_000759"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9317096.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            21355..21939
                     /locus_tag="ACHBFW_000760"
     CDS             21355..21939
                     /locus_tag="ACHBFW_000760"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM9317097.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21942..23015
                     /gene="prfA"
                     /locus_tag="ACHBFW_000761"
     CDS             21942..23015
                     /gene="prfA"
                     /locus_tag="ACHBFW_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFM9317098.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23008..23847
                     /gene="prmC"
                     /locus_tag="ACHBFW_000762"
     CDS             23008..23847
                     /gene="prmC"
                     /locus_tag="ACHBFW_000762"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFM9317099.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23840..24019
                     /locus_tag="ACHBFW_000763"
     CDS             23840..24019
                     /locus_tag="ACHBFW_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317100.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24041..25066
                     /locus_tag="ACHBFW_000764"
     CDS             24041..25066
                     /locus_tag="ACHBFW_000764"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFM9317101.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25257..26501
                     /gene="glyA"
                     /locus_tag="ACHBFW_000765"
     CDS             25257..26501
                     /gene="glyA"
                     /locus_tag="ACHBFW_000765"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFM9317102.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26654..27601
                     /locus_tag="ACHBFW_000766"
     CDS             26654..27601
                     /locus_tag="ACHBFW_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFM9317103.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27735..28364
                     /gene="upp"
                     /locus_tag="ACHBFW_000767"
     CDS             27735..28364
                     /gene="upp"
                     /locus_tag="ACHBFW_000767"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFM9317104.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28503..28745)
                     /locus_tag="ACHBFW_000768"
     CDS             complement(28503..28745)
                     /locus_tag="ACHBFW_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317105.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28866..28938
                     /locus_tag="ACHBFW_000769"
     tRNA            28866..28938
                     /locus_tag="ACHBFW_000769"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28899..28901,aa:Thr,seq:cgt)
     gene            complement(29057..>29636)
                     /locus_tag="ACHBFW_000770"
     CDS             complement(29057..>29636)
                     /locus_tag="ACHBFW_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFM9317106.1"
                     /translation="VVLGLLGLLFAPSIISIFSLTITGFIAFTLVFGKKDTKKMFSKP
                     VAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPINEAFSPLLFIILPIMILGEE
                     LFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNGNILHTVAHILLIQGVARLLF
                     NQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT        10092 a         5227 c         6043 g         8274 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gtctgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt ctggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccatgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaacgtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg acgaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcggaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggcag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatcca gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaagag
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttcgct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata
    13261 gggcagatcc aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc
    13321 caggttctca tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta
    13381 aatgacacct tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa
    13441 aggtcgttca ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt
    13501 ttcattgaat tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact
    13561 taacgtgtag tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta
    13621 gtagtgacct agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat
    13681 atagttgatc aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa
    13741 gtcttgacca ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat
    13801 actaacgatc tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa
    13861 aaggtttccg ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag
    13921 ttcataatta gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac
    13981 gaagctgcaa tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt
    14041 tgattttttc gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc
    14101 cggcaacttt tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg
    14161 ggatatccat atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc
    14221 cgtctgcgta attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa
    14281 tcatatcgat tacttgattg atacctacag gatttttgac aagtactaat gtacattttt
    14341 tcccattgat attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt
    14401 gtttgatttt atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat
    14461 tgtagacatt atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat
    14521 ctgctgacac attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt
    14581 tgaagtcaca atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat
    14641 gcaagatgtg atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt
    14701 cacgatcttc ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat
    14761 tgaagattgg tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt
    14821 ctacaatcat tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa
    14881 tatttgtaaa taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt
    14941 catctatttc taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag
    15001 tcgatacaat tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt
    15061 gctggcgcaa aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga
    15121 caacgacgac ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg
    15181 ctagtttacc tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg
    15241 tttttccaac tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga
    15301 tttgctgacg aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt
    15361 ttcttattct taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga
    15421 tttaacaaaa aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt
    15481 ccctcttacc tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc
    15541 ctagatctcg tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc
    15601 caataaacat cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc
    15661 ctaaagataa accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg
    15721 tttgttcttc tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca
    15781 taaagacaac gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga
    15841 tgttatcttc acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg
    15901 ttcgaatccc gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa
    15961 tgatcagtgt tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag
    16021 tcgtaatcga ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata
    16081 aatggtcctt ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag
    16141 ctaacacagc taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt
    16201 tgtataatag ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa
    16261 tcaaaaacat gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc
    16321 cagtgacgtc aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa
    16381 cggcatcacg ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc
    16441 caaaaagtaa ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac
    16501 cagacaaacg cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc
    16561 gtcgtttaga aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt
    16621 gaaataatat ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag
    16681 tcgatagtcc agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa
    16741 catggctatc tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca
    16801 tcaatatata ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct
    16861 gtcctcgccg ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac
    16921 aatagtcgtt tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca
    16981 gataacaata cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa
    17041 aaagaaggag gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa
    17101 atcaaaaaat aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag
    17161 aagtaaagcc taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg
    17221 aattcatgtc tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta
    17281 aaagtgagta tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga
    17341 aaactgacca tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa
    17401 tggttttaac aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct
    17461 tcaatatgcg attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg
    17521 gaagtgccct gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg
    17581 atatccaaaa gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct
    17641 atgaacgctc tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt
    17701 gcgtgttcgc tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag
    17761 cttacttaaa aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac
    17821 gtaagaaaaa aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag
    17881 taaagtaagg aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca
    17941 cctattttga agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt
    18001 attgtgaata gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca
    18061 acaccaacta cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc
    18121 actacgacaa gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca
    18181 actgacagct caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc
    18241 aatccaagta caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact
    18301 gtagatgata acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca
    18361 ggaaataccc aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc
    18421 ttatcaacgg ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc
    18481 gtggatatac gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac
    18541 tttggcaaaa ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg
    18601 tttattttac tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg
    18661 tcactcagct ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat
    18721 cgatagataa tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg
    18781 acgaagatta tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga
    18841 tcaaaacaaa tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc
    18901 cttattacta tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct
    18961 actcggacat ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac
    19021 ctgtcggatt gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc
    19081 cttatcgtct gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata
    19141 gtcgtagcca aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg
    19201 ataaaaacgt ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt
    19261 atataacgag aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt
    19321 gtagaataaa gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat
    19381 acaagtagga ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta
    19441 ttagcggcta ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat
    19501 tcgatggacc ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat
    19561 gaacgaatgg atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa
    19621 cggatcatcg gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat
    19681 ggcaaaaagt atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag
    19741 ttacaaggag aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc
    19801 tttacgagtg actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac
    19861 cgcttgattt caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga
    19921 aaagttgttt tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa
    19981 aagaagctgt gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct
    20041 tggagttgct cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa
    20101 gcgaagcgtt tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag
    20161 agacaaagcc cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga
    20221 ctttcggatg actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag
    20281 gagctaaaat ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta
    20341 caagtgatag aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt
    20401 ggatcgttcg gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca
    20461 tagtttctga agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa
    20521 caagcccagt tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat
    20581 aagtagttag ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat
    20641 ggttagcgat ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg
    20701 aaattttaac ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa
    20761 gggagaatct agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa
    20821 gtgtgtccga gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga
    20881 aaaatatatc gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata
    20941 tttaggcttt atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt
    21001 atgggaataa aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca
    21061 gaaggttacg cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac
    21121 tactctatat atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa
    21181 gtttttccaa atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa
    21241 caaaagtgag caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa
    21301 atgctgaaaa tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca
    21361 caactatttt ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc
    21421 gcacataatt atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca
    21481 agagatggtt taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata
    21541 tttgaagaaa cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg
    21601 ctgatcgatg aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg
    21661 gacgaattgg atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta
    21721 tttgaaggat caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc
    21781 tgttggttct gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt
    21841 tatgaaggcg atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa
    21901 cattactttc acccatcaat gattacagaa ggagaataag catgtacgat caattacaag
    21961 caattgaaga ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg
    22021 atacaaagcg ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag
    22081 tttatcgtcg ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg
    22141 aaaaactgga tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag
    22201 aaaaagaagt cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg
    22261 ataaaaatat catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg
    22321 ctggcgatct tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag
    22381 tgatggaagc taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg
    22441 gagacaacgt atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc
    22501 cttcaactga atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag
    22561 aagctgaaga agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg
    22621 cttcaggtgc tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact
    22681 taccaacagg gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa
    22741 aagcaatgaa aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg
    22801 agtatgatgc caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga
    22861 cgtataattt cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat
    22921 tagatcagat tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc
    22981 aaacatcaaa actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca
    23041 cgggcttctt cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt
    23101 ttggaacgaa aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca
    23161 gaagaagaac agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct
    23221 cagtatttgc taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca
    23281 ctgatccctc gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt
    23341 gtaccgttag aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa
    23401 gcagcaagaa aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta
    23461 gcgaaagaaa atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta
    23521 ggaccagtaa tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg
    23581 aatgaatggg aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt
    23641 gctgagaacg atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta
    23701 aaatcaaatg gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa
    23761 atatttcagc aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat
    23821 gaacgaatga tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt
    23881 tattataaaa aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt
    23941 ttatccacta ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag
    24001 acaaaataag aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga
    24061 tgaaacagaa ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc
    24121 taccgaaaca gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt
    24181 gttttctgta aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga
    24241 acaagtaaaa gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt
    24301 ctggcctgga ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt
    24361 gacgggagga ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt
    24421 aatcgaactt tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag
    24481 tccaacgact gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga
    24541 tgatggagct acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc
    24601 catgcctatg attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga
    24661 atctcctgtt gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg
    24721 gatgaaatac aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg
    24781 gtcaactgca gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc
    24841 cgaaattgcg gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc
    24901 agtagaagcg gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact
    24961 tggattagat ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat
    25021 gaatcgcttg aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg
    25081 tcgcattctt ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa
    25141 ctaagcgtaa ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg
    25201 aaaaataaaa ttgtaggaaa ataaaattgt aggaaaataa aaaggagctg ttgtttgtgg
    25261 tagattacaa aacgtttgac cctgatcttt gggctgcgat cgccaaagaa gaagaaagac
    25321 aagaacataa tttggagctg attgcctctg aaaactttgt ctccgaagca gtgatggctg
    25381 cacaaggaag tatcttgaca aataaatacg cagaaggtta tccagggcat cgctattatg
    25441 gtgggtgtga atttgtcgat atcgttgaaa atttagcgat tgaccgtgca aaagaattat
    25501 tcggagcgaa atttgcgaac gtccagcctc actccggctc tcaagccaac acggctgctt
    25561 atctcgcttt ggtagaacca ggggacacga tcttaggaat ggatctttcc gcaggcggac
    25621 atctaacaca tggatcacca gtcaacttca gtggcaaaac ctatcacttt gttgcttacg
    25681 gtgttgatcc gacaacagaa gttattgatt ataatgtggt acgtatttta gctagaaaac
    25741 atcaaccaaa attgatcgta gcaggtgcca gtgcttacgg acgaacgatc gactttgcga
    25801 aattcagaga aatcgcagat gaagtaggcg caaaactgat ggtagatatg gcacatatcg
    25861 caggattagt agcagcagga cttcatccta atccagttcc ttatgcagat atcacaacga
    25921 ctaccactca taaaacattg cgtggccctc gaggcggcat gattttgaca aacgatgaag
    25981 cattagcaaa aaaaataaat agtgcggtat ttcctggtat tcaaggcggc cctctagagc
    26041 atgtgattgc gggtaaagca gtagcattca aagaagcatt agatcctgca tttaaagaat
    26101 atagtgaaca aatcatcgcc aatgcaaaag caatggtcaa agttttcaat caagcaattg
    26161 gaaccagagt catttcaggg gcgacagata atcatctgat gttgatcgat gttcgcgagt
    26221 taggtataaa tggaaaagaa gcagagagca ttttggacag tgtgaatatc actgtaaata
    26281 aaaactcgat tccttttgaa acactaagtc cattcaaaac aagcggtatt cgaatcggca
    26341 cgccagcaat cactactcgc gggttcaaag aagaagatgc tgtaaaagtg gctgaactag
    26401 ttgtcaaagc attacaagca aaagatgata acgctcaact agacgaagtc aaaacaggtg
    26461 tacgcgaatt aactgaaaaa tttccgttgc ataaaaaata aaaatttaaa ttccattagt
    26521 aaacagaaaa gttcaatgtt gggcttttct gtttattttt tgggaaaacg gattctttga
    26581 caatatcggt gatgctctct agtatatgag gtgtaatttt agtcttcaca ctaaatctca
    26641 gggaggaaaa gtcatgactg atcgtatctc gttagaaaag gaagcagtag cattcagtga
    26701 tgcaaatgcg cctcatccgc gtatctatga actagcaccg gaagaaggaa gagttttact
    26761 agaagaagta caagaatcac ctgtgaacaa atattccgtc gatattgaag acttgatggt
    26821 ggatactggc gaatggggaa aactcaatat tcgtctcgta cgaccagaag ggaatatgga
    26881 taaattacca gttatttttt atattcatgg agcaggctgg gtttttggca gtgctcaaac
    26941 ccatgataaa ttgatcagag aacttgcggt tcgtacgaat tccgtcgttg tctttccaga
    27001 atatagtcga tcaccagaag caaaatatcc aacagcaatt gaacaaaatt atgctgtgtt
    27061 gcaacagctg aataaattag ccaagtcaaa aaatctggat ctcgagaatc taatcgttgc
    27121 aggagattca gtaggtggta acatggctac agtcatgacg atcttaacaa aagaacgaaa
    27181 gggcctccca atatccaagc aattgctgta ttatccagtc acaaacgctg attttgatac
    27241 agattcctat catgaattcg gagaaaacta ttttttgaca agagaaggaa tgatgtggtt
    27301 ttgggaccaa tatacgacga atgaagaaga gcgtgctgaa atcacagctt cgcctttgag
    27361 agcaaatata aatgaactaa atgatcttcc ttcagcaatg attttaacgg gagaagcgga
    27421 tgtattaaga gatgaaggtg aggcttatgc gcgcaaactt cgtgatgcag gggttcctgt
    27481 tacacaagta cgcttccaag gaatcatcca tgattttgtc atggtaaatg ctatggatca
    27541 gacaaaggca acgcgtgcag caatggcatt atctacttct tggatcaaga aaaaagaata
    27601 agctttgctt gtccgcagga aaacttcttt tcaaaaacgg attttcttag gaaaaatggc
    27661 gagtttgact agtcaatcac ttgattttgt tttacaatta aaaagaaacg acagaacaaa
    27721 ggagagctta aaaaatgggc aaatttcaag tgatcgatca tccattgatt caacacaaac
    27781 taactattat tcgggacaaa aactgcggaa caaaggtttt tcgtgaagta gtcgatgaaa
    27841 tcgctatgtt gatggcatat gaagtatcac gggatatgcc attggaagac atcgtgatcg
    27901 aaacaccgat cactgaaaca acacaaaaaa cgttgtctgg taaaaaagta gcgattattc
    27961 caatcttgcg cgcaggtatt ggaatggttg acggtatctt ggaattgatt ccagctgcta
    28021 aagtgggaca tatcggtctt tatcgagatg aagaaacatt ggaaccacat gaatatttcg
    28081 tcaaacttcc agaagacatt gatgcacgtc agttgtttgt agtagatcca atgttggcaa
    28141 caggcggatc agcaatcatg gcaatcgatg cattgaaaga acgtggcgcg tcgaacatta
    28201 aatttgtttg tttggtagca gcacctgagg gagtaaaagc tttgcaagaa gcgcatcctg
    28261 atattgacat ctatacagct tctctagatg aaaaattgaa tgaacatggt tatatcgttc
    28321 ctggactagg ggatgcgggt gaccgtttgt ttggtacaaa ataagtaaat acgaaaaagg
    28381 gtgtgacaca acttttgtca catccttttt tggtcatttt taacgtttga tgcagaacag
    28441 ctttttcaca gctttcgttt gcatataaaa aaaccatccc aaaaatgcag agatggtcaa
    28501 attcaaatct tttttagttc tttgtagtta acgattacta cacccttact ttgcgtcatc
    28561 aagttcgcat tttctgcagt ttcatcaatc tcaacaacag cggaattttt caattgcttt
    28621 gaaatcgttc cagtttgcga cttgccgtga attgtgaaca ttacgtgagt attgatcttg
    28681 aatttttcac caatttccga tggtacgata gttgtatcaa attttccaaa agttgccatt
    28741 ttcataaaac ctgcctttca catcaattat accacgtttt atgaaaaata tcatgaaatt
    28801 aaactaattt tccatcggac tctttacaat cagccaaata gagtatataa tttcttttgg
    28861 ataaggccag cttagctcag ttggtagagc aacgcactcg taacgcgtag gtcgtaggtt
    28921 cgatccctat agctggcatt aaaagagaag acagatatat caaggatttt taatccattg
    28981 atacatctgt ctttttgttt aaaggaaata aagtatgctt tcttagcaat agagcatgta
    29041 ttatttctat aaaaatttag gagaataaaa cgactagcaa tatagctgtg aaatcaaata
    29101 tcacatgaac tgcccaactt gtaatgatcg agttgctttt aatggcagct tggttgaata
    29161 acaatcttgc caccccttgt atcaaaagga tgtgtgctac agtgtgtaaa atatttccat
    29221 tatcataagt actgtaatgg ataaatccaa agatgattgc tgacaagaaa ctagcgacag
    29281 gaatgctgaa cttactagaa aaaatagcga gaaaatacac tgagaacaat tcttctccaa
    29341 gtatcatgat aggcaaaata ataaataata acgggctgaa agcctcattt attggattgc
    29401 ctgttaaacc ccatttcaaa atttgttgca aaaataccga gactgcggca ctgattacta
    29461 cattgataaa aaaatatttg gctatatttt tgacaggagc gacaggtttc gagaacattt
    29521 ttttcgtatc ttttttacca aaaacaagtg taaatgctat aaatccagtg atagttaaag
    29581 aaaaaatgct aataatggat ggcgcaaata gcaaccctaa caaaccgagt acaacg
//
LOCUS       DAYYME010000021        29100 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000021 DAYYME010000000
VERSION     DAYYME010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.021"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1031)
                     /locus_tag="ACHBFW_000771"
     CDS             complement(<1..1031)
                     /locus_tag="ACHBFW_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9317107.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSVVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="ACHBFW_000772"
     CDS             complement(1113..1802)
                     /locus_tag="ACHBFW_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM9317108.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACHBFW_000773"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACHBFW_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFM9317109.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACHBFW_000774"
     CDS             complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACHBFW_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFM9317110.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACHBFW_000775"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACHBFW_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFM9317111.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACHBFW_000776"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACHBFW_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFM9317112.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACHBFW_000777"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACHBFW_000777"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFM9317113.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACHBFW_000778"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACHBFW_000778"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFM9317114.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7544)
                     /locus_tag="ACHBFW_000779"
                     /pseudo
     CDS             complement(6444..7544)
                     /locus_tag="ACHBFW_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7636..8523)
                     /locus_tag="ACHBFW_000780"
     CDS             complement(7636..8523)
                     /locus_tag="ACHBFW_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9317115.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACHBFW_000781"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACHBFW_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFM9317116.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACHBFW_000782"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACHBFW_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFM9317117.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACHBFW_000783"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACHBFW_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM9317118.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /gene="cbpA"
                     /locus_tag="ACHBFW_000784"
     CDS             9754..10398
                     /gene="cbpA"
                     /locus_tag="ACHBFW_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFM9317119.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="ACHBFW_000785"
     CDS             complement(10454..10957)
                     /locus_tag="ACHBFW_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM9317120.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="ACHBFW_000786"
     CDS             11554..12519
                     /locus_tag="ACHBFW_000786"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM9317121.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="ACHBFW_000787"
     CDS             complement(12618..13790)
                     /locus_tag="ACHBFW_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9317122.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="ACHBFW_000788"
     CDS             complement(13777..14922)
                     /locus_tag="ACHBFW_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9317123.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="ACHBFW_000789"
     CDS             complement(14936..15868)
                     /locus_tag="ACHBFW_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9317124.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="ACHBFW_000790"
     CDS             15985..17064
                     /locus_tag="ACHBFW_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM9317125.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17679
                     /locus_tag="ACHBFW_000791"
                     /pseudo
     CDS             17061..17679
                     /locus_tag="ACHBFW_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(17789..18016)
                     /locus_tag="ACHBFW_000792"
     CDS             complement(17789..18016)
                     /locus_tag="ACHBFW_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9317126.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18119..20707)
                     /locus_tag="ACHBFW_000793"
     CDS             complement(18119..20707)
                     /locus_tag="ACHBFW_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFM9317127.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20727..21386)
                     /locus_tag="ACHBFW_000794"
     CDS             complement(20727..21386)
                     /locus_tag="ACHBFW_000794"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9317128.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLLNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACHBFW_000795"
     CDS             complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACHBFW_000795"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFM9317129.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22159..23025)
                     /locus_tag="ACHBFW_000796"
     CDS             complement(22159..23025)
                     /locus_tag="ACHBFW_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM9317130.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23134..23775
                     /locus_tag="ACHBFW_000797"
     CDS             23134..23775
                     /locus_tag="ACHBFW_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFM9317131.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACHBFW_000798"
     CDS             complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACHBFW_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFM9317132.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25216..26115)
                     /locus_tag="ACHBFW_000799"
     CDS             complement(25216..26115)
                     /locus_tag="ACHBFW_000799"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM9317133.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26117..26914)
                     /locus_tag="ACHBFW_000800"
     CDS             complement(26117..26914)
                     /locus_tag="ACHBFW_000800"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFM9317134.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26892..27596)
                     /locus_tag="ACHBFW_000801"
     CDS             complement(26892..27596)
                     /locus_tag="ACHBFW_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFM9317135.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27727..28302
                     /locus_tag="ACHBFW_000802"
     CDS             27727..28302
                     /locus_tag="ACHBFW_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFM9317136.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28420..29097
                     /locus_tag="ACHBFW_000803"
     CDS             28420..29097
                     /locus_tag="ACHBFW_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFM9317137.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8564 a         6167 c         5230 g         9139 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactac acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctag ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    17221 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    17281 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    17341 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    17401 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    17461 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    17521 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    17581 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    17641 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    17701 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    17761 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    17821 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    17881 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    17941 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    18001 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    18061 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    18121 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    18181 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    18241 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    18301 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    18361 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    18421 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    18481 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    18541 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    18601 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    18661 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    18721 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    18781 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    18841 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    18901 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    18961 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    19021 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    19081 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    19141 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    19201 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    19261 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    19321 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    19381 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    19441 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    19501 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    19561 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    19621 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    19681 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    19741 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    19801 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    19861 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    19921 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    19981 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    20041 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    20101 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    20161 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    20221 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    20281 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    20341 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    20401 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    20461 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    20521 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    20581 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    20641 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    20701 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    20761 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    20821 gtctctaaaa tcgtcaaact actatttaac aatccaccca tttcacgcaa ctcacttagc
    20881 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    20941 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    21001 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    21061 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    21121 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    21181 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    21241 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    21301 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    21361 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    21421 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    21481 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    21541 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    21601 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    21661 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    21721 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    21781 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    21841 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    21901 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    21961 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    22021 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    22081 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    22141 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    22201 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    22261 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    22321 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    22381 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    22441 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    22501 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    22561 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    22621 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    22681 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    22741 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    22801 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    22861 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    22921 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    22981 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    23041 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    23101 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    23161 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    23221 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    23281 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    23341 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    23401 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    23461 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    23521 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    23581 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    23641 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    23701 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    23761 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    23821 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    23881 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    23941 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    24001 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    24061 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    24121 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    24181 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    24241 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    24301 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    24361 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    24421 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    24481 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    24541 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    24601 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    24661 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    24721 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    24781 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    24841 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    24901 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    24961 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    25021 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    25081 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    25141 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    25201 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    25261 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    25321 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    25381 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    25441 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    25501 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    25561 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    25621 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    25681 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    25741 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    25801 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    25861 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    25921 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    25981 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    26041 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    26101 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    26161 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    26221 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    26281 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    26341 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    26401 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    26461 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    26521 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    26581 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    26641 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    26701 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    26761 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    26821 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    26881 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    26941 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    27001 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    27061 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    27121 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    27181 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    27241 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    27301 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    27361 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    27421 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    27481 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    27541 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    27601 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    27661 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    27721 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    27781 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    27841 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    27901 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    27961 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    28021 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    28081 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    28141 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    28201 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    28261 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    28321 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    28381 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    28441 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    28501 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    28561 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    28621 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    28681 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    28741 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    28801 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    28861 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    28921 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    28981 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    29041 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaaa
//
LOCUS       DAYYME010000022        29088 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000022 DAYYME010000000
VERSION     DAYYME010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.022"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACHBFW_000804"
     CDS             complement(296..1024)
                     /locus_tag="ACHBFW_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9317138.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACHBFW_000805"
     CDS             complement(1002..2576)
                     /locus_tag="ACHBFW_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM9317139.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACHBFW_000806"
     CDS             2805..3743
                     /locus_tag="ACHBFW_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9317140.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACHBFW_000807"
     CDS             3755..4666
                     /locus_tag="ACHBFW_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9317141.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACHBFW_000808"
     CDS             4690..6162
                     /locus_tag="ACHBFW_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9317142.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACHBFW_000809"
     CDS             6213..6824
                     /locus_tag="ACHBFW_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM9317143.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACHBFW_000810"
     CDS             6854..8767
                     /locus_tag="ACHBFW_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFM9317144.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACHBFW_000811"
     CDS             8895..11876
                     /locus_tag="ACHBFW_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFM9317145.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACHBFW_000812"
     CDS             11873..13534
                     /locus_tag="ACHBFW_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFM9317146.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACHBFW_000813"
     CDS             13574..14506
                     /locus_tag="ACHBFW_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFM9317147.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(14898..16445)
                     /locus_tag="ACHBFW_000814"
     CDS             complement(14898..16445)
                     /locus_tag="ACHBFW_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFM9317148.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(16547..16900)
                     /gene="tnpB"
                     /locus_tag="ACHBFW_000815"
     CDS             complement(16547..16900)
                     /gene="tnpB"
                     /locus_tag="ACHBFW_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM9317149.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(16890..17084)
                     /locus_tag="ACHBFW_000816"
     CDS             complement(16890..17084)
                     /locus_tag="ACHBFW_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317150.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(17310..18512)
                     /locus_tag="ACHBFW_000817"
     CDS             complement(17310..18512)
                     /locus_tag="ACHBFW_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9317151.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(18515..18709)
                     /locus_tag="ACHBFW_000818"
     CDS             complement(18515..18709)
                     /locus_tag="ACHBFW_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM9317152.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(18756..19604)
                     /locus_tag="ACHBFW_000819"
     CDS             complement(18756..19604)
                     /locus_tag="ACHBFW_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFM9317153.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(19809..20159)
                     /locus_tag="ACHBFW_000820"
     CDS             complement(19809..20159)
                     /locus_tag="ACHBFW_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317154.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            20337..21200
                     /locus_tag="ACHBFW_000821"
     CDS             20337..21200
                     /locus_tag="ACHBFW_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9317155.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            21824..22816
                     /locus_tag="ACHBFW_000822"
     CDS             21824..22816
                     /locus_tag="ACHBFW_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9317156.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            22832..23716
                     /locus_tag="ACHBFW_000823"
     CDS             22832..23716
                     /locus_tag="ACHBFW_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9317157.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            23765..25372
                     /locus_tag="ACHBFW_000824"
     CDS             23765..25372
                     /locus_tag="ACHBFW_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9317158.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            25373..26857
                     /locus_tag="ACHBFW_000825"
     CDS             25373..26857
                     /locus_tag="ACHBFW_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFM9317159.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            27034..28026
                     /locus_tag="ACHBFW_000826"
     CDS             27034..28026
                     /locus_tag="ACHBFW_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9317160.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            28078..28947
                     /locus_tag="ACHBFW_000827"
     CDS             28078..28947
                     /locus_tag="ACHBFW_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9317161.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
BASE COUNT         9179 a         4769 c         5868 g         9272 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagttgaaa
    14821 agtaagcgcc ccatagaaca gtacctgtac gtcaaataac ccgacctata gaatagaatc
    14881 gggaatgttt ggcgttttta gttacgagaa aaatgttgtt gtacatcctt gttccaaggc
    14941 aacagttgat ctaaagtttc tgctgaaagt acgggaagat ttggtatctt atcaaataga
    15001 aactctagat actttacagg attcaatccg ttaagttctg ctgttttata tacactcaga
    15061 atgattcctg aagttcgtgc tccttcaaga cttgtggaat gcaaccaatt tttccgtcct
    15121 atcaccagct ctttgatatt tcgctccgca cgattatttg tgagttctaa acgaccatct
    15181 tcaaaaatag gttcaaaacc acttcgtagt ttacacgcat attgaacggc tgcatccaac
    15241 ttggattttg caacaggatc tatcgttgcc atccactcat aaaaagcaat aaataatgga
    15301 cgcagttctt gttgccgaca gcttaatcgc ttctcaggag aaaagtcttt ccatttcttt
    15361 tctaggacaa accatttatc caattgtttg actgcttgag ctgcgggaat atccgtgttt
    15421 ttctttcctt ttggaattgc ttcgaaaaat ttccgccgaa catgcgcgaa acaatagaca
    15481 agtcgtacac tttccacggc attatacccg ctatagccgt cacagtgtaa ataccctgta
    15541 aactccttga gaaaagtttt cggaacatct ccagcacgac tttcgtcgtg gtgataataa
    15601 acgatgggat gctgactgtc ttttcctgta caaaactgcc agtaataggt atctgttttg
    15661 gcactttcaa tcacacgata acttgtttca tccccgtgta gaatctcttc ttgattaaga
    15721 tagactctta atcgttctcc taaagggtct aacgcgtttt gcactgcttt aatatgccaa
    15781 ttggtgatat tatctctcga aatatccaac ccatgtgttt gtttccaata gttctcttgc
    15841 cgataggcag gaactttctg accaaatttc atccggatcg tttccgccac taaactattg
    15901 gaacctaagc tattagaaat caaaggttta ggaacgatcg cttttttaaa agaagaaagt
    15961 ccttcttttt cgcagctttt acaacaatag gtatgttgat aatgggtgag acaatccaac
    16021 atagcctgat ggaaacgaat ttcttctcgt acttttgtcc gtccgatatc tttcatctgt
    16081 gaaccgcaat gttcacaaaa ttgttcttcc aaaggaagga gatgttcttt ctctgttata
    16141 ggtaagtgtt tgattttgtc tgctttcgtt cctacaactc gttgatgtcc ttttactgga
    16201 ataatggtgg cagctgcttc tggaacactt acagaaattt cttcatcact aaaaagattc
    16261 aattgcccat tcgtttcctc aaagagagtc tctttagagc gtccaaataa tttgttggtt
    16321 aaataattga ccttttcatt caatagttgg atttcagttg taagattctg aatggtttgt
    16381 tgttgtgctt gattggtttt atgggcttct tgaagcaact gaagcaacgt atctgtagtt
    16441 tccaaaattt ttcaccacct tgaatcgaac tgattttgtt acagaatacc acaagaaacg
    16501 cacaagaaat aggggttcaa cacatttttt gtgttaaccc cccagcttaa aaaactcctt
    16561 tttttcctgg caaaatagct cgaggttgat cgatacttaa tccttctaac aaccatcgaa
    16621 cctgttggtt cgttaaggtt cgaacttcgt ctttcgtccg aggccatttt aacttgccgt
    16681 tttctatccg cttataacag agaagaaaac catcaccgtc ccaatagaga agtttgaaac
    16741 ggtcttgacg gttcccgcaa aataaaaaaa cggcatcttc gtaaagctcg agttgatact
    16801 cttgttgaat aagactggca agaccatcaa ttccttttcg caaatctgtt tttccgcaca
    16861 caatataaat atgtttggtt tgaagtaaat cataaagcat tggacaacag ttctttcaaa
    16921 atagcttgaa gaatataatt gttaataccg ttatagatca aaacttcaga accattctct
    16981 cttttgatgc gcatcgctaa cttggcagcc gcgggtttac ctttttctgg tgcactcttt
    17041 tcgtttccta attggacagg aacaatctca tttaatttat acatagaaaa cctccagact
    17101 tcttgatagc tcaagtatat cttgaggtgc atttgttagt aaggtactgt tctatggggc
    17161 gcttacgttg aaaaacgttg atttatcggc attccccatc catattatat ttacgtctta
    17221 ttcccactca atatgagtag cctcgtaaag aagctattat atcagtgctt tttcagccta
    17281 ttcctcattt tggtagggaa atggtagggt tagattttta ctgcttgatt tccattaaag
    17341 tttgcaactt tagattttgt tgcacaggta aatttaatgt ttcctgtcat ttgttctgca
    17401 attccttcat cattacggct ccatagatga gcgtagtgct ttaaagtgat ttcagggcta
    17461 gaatggccta accgttgcga cacgaccaga atgtctgcgt taaactcatt gattaggtag
    17521 ctaacatgag aatgacgtaa tccttttcct tgtattaggg gtaccttagc tagtttggca
    17581 taacgctgaa taattcgttg aacagttgaa cgatagagtg ggaggtcagt gtagctaaga
    17641 ataaattgtt ctacaccatg atttttttga ataactttcc acttttttag cacttcaatt
    17701 gtatcatcgt ccagagaaat gatccgcaag ccgctttcag tttttgtgta aggtttccga
    17761 ataaaatctt tttgatttgt catttccaag gtgtgatgaa ccctcaactt tttacgtttg
    17821 aagtccacgt cattccaatt aagtgctaat ccttcgctta cacgaacacc agtcatgtaa
    17881 tatagccaga tcattaaaaa acacatatgt tcgtagaaat catctttata aatttttgcc
    17941 aaaacttctt caaattcttc ttttgtccag taaggaacga ttgattttcc ttttggaatt
    18001 gcttttgttc ttttggaaat attctcattc agaaattgca gggtgaccgc atagtctaat
    18061 gatttacgaa acataccgta cataagtgaa ctgtatgctt gtgagtatcc gctatcgttc
    18121 aataaccaaa ttcgataatt ttcacaatct cgcacattga tttcacgaag cttcttatca
    18181 ccgaaaaatt cagtaatctg aatcaatcca cactcacgag aactccaagt acttttttgt
    18241 acgctagctt tgtagtgtgg aacgtaagac tgctccataa attctttgta agttaaatca
    18301 taattcgcaa atccatttac aaccatgtat tcatttttta tacgcatgac ttctttgtgt
    18361 gcttcacgag ctgatgagaa tcgttttcct agagaatctt ttgttccttt cttctggatc
    18421 ctctttccag tcacttgatc tgttcctaac tcaactagat aagaaaagcg accattttta
    18481 tcttgataca cacaagggta ttttgttttc atagttattc ctcctgtgaa agaggaacac
    18541 ctataatttc ttcaattact tgtcggggta ctaccccaac tcttttatta ttataatatg
    18601 gatatccctg atttaccatt cttgtttttg cttgtcggat tattgtggta gcttgatgtg
    18661 ttttaaatcc tagagcctct aaatcttttc gacaaattgt atttttcatt gtataaactc
    18721 actttcaaag aaaacactat cattgggtaa tagtgttatt tctttatttt tgtataatga
    18781 acggtacttt cttgaaaatc catcacttgt tcaggacttc ccaactcatt taaagctaaa
    18841 gcattgtaaa aaaatggacg tctaactata tattcgcttg tatatagtat atcttgattc
    18901 gtggaatcat agggtttctt taccatcttt ttggtgacag ttggtagttt gagattccgg
    18961 gacttgaaaa aagctttttt cttatgctct ttcaattcca gctctttatc gaaatatttg
    19021 ctgatatagc gcccgcgatt ttcgatactg tctacatcga tcttgttgat cttaacgaaa
    19081 ccctgacccc aaatcgtagt taaccggtta aatgagataa aggggaaatc aaacaagata
    19141 atatggtagt ggattgcacc gcgttgctgc ttttcccacg tggcgagata ctttaaattt
    19201 gcttttttag ttttatagag gtcataattc agtcgtttga taaatttttt aaattcatta
    19261 tttgcatact caatgtcttg aatgttttct ttgaaagtga gagttaaaaa tttcgtttga
    19321 ttgtcaaaat tcgtgtctac aaggcgggca atctcaaatc gcatactttg ataatgtttc
    19381 tgtttgcgtt ttaaactgtc atattgtcct tgagcggata attcatcata gttttttctt
    19441 ttttcaagag taatatcatt ttcataaatc caagctaatt tttgttttcg ttccagattt
    19501 acttcgtttt cgatcgtttt acgtttggga gtaatgattg cttcataatc ataaatttca
    19561 atatacgtgg gagtttcaat gattttctga ttataagctt gcaaaaatca tcactccttg
    19621 ttgcgtattc gattgtcaaa gaaccagtca ttttctcctg atgttgatct ataaatcaag
    19681 ttaaggggga aggcctgcgt agcagccctt ctagcctatg gggtgttggg cggtcttaac
    19741 ggacagaaac ggctctgtgg gcgctgcccc gccgtttcgt cctaaccacc caacactcca
    19801 tagtatagtt agttttgtgt agtggtacct tgttttctgg gaatcaaagt tgccgtatca
    19861 gcggttgccg ataacccata actgacaaat tgtccattcg agcgtcccca aggtacaatc
    19921 accaaattgg taaatgtaat tatggctaaa ccttgttggg taagttcttc cggcgtgatc
    19981 acaggatgtt cagatttgac tttgacactg attttttcgt agcgaagttt tggtaggata
    20041 cattcgtagc tccaacctag aatttcgccc tccttttgcc agcgagtgac attgacgact
    20101 tggtattctt tttcgagtgt ttcttttggt aaactgagat cggtaatttt tattggcatc
    20161 acaattctcc ttttttatac gaaagtttat acgatttgat taaaaaatat agatggccat
    20221 ctacgacttg tattatatac gagaacttat acgtagtcaa gagattttct aaaaaaatat
    20281 atacgatttt ttatacgagt gttataatga aaaaaatgat aaggaggaat ttgaatatgt
    20341 ttggtttagt acaacctaat aaagtgaagg tgggccaacg aatcaaagaa ataaaagacg
    20401 gaatgtctct ctcttttaca gaattaggga atcgattagg tttaaaaaag ccgacgatca
    20461 gttcctacgt tcaaggctat gcgttggctc ctaagagtgt gatcaaccaa ttatctagta
    20521 tcagcggaaa acctgtcggc tggttttatt ttgggcatgt ggaagaatat atccgagagt
    20581 atttgtttct cattggtcag aaaaaaatag tggacgatta tcctgaaatc attgagaaaa
    20641 taaagcaaga attttatacg ggcgagttca aaaatcctgc ttgggagaat gagctaggct
    20701 atcctttgga ggaatttatc caagaatgtt ttgtagaata ttcgagtgaa atcatgaagg
    20761 actatgtcgt acagattacg aaagaggtac ttgaagaatc caaaacgcta aacaaactat
    20821 catcaaaagc aaaagaagaa gctgtcacga ttctttcatc tgggatcatt gactatatcg
    20881 agatgagtgg tgagatcaag tatggagaga aagatcgcat tgcgaaaatg gtgaaagaat
    20941 caataaaaac ctttgatgtg aaacaagacc tgacatttaa tgattcctat ttgattggaa
    21001 agttgatcaa tattcttggt acagatgaag atacggagct gtttctttct tctttatcaa
    21061 tgatatttac tgataagcat ttttccactc gatttggtgg ggaagaatta gtcgaaatat
    21121 ttcaatcgat gcgaccagcg ttaatcaaac tctatcggga gaaaacacca gatgaatttt
    21181 atgattggtt tgagaagtaa aataagaaaa gtggcttaga ttacaagttg gtgaaaaaat
    21241 ttttttcagc ataacatcag taatctataa aggggttgtt gattatactg tagaagttaa
    21301 gtactcaaga gattttttgt acgtaaacaa agcaaaaaag ggtgcagttt cactagaaaa
    21361 tgtactcttt tttgtgattt ttcttagtta tagagttttt ttgaaacttt aaaaatgaga
    21421 aaacatattg attttaaaga gtgtagacat agcttctttt gctaaataga ttaaacttac
    21481 tggtgagtgg tcttgaacat acttttaaat cacattttac tgagttttat tggattttat
    21541 aattcattgg aaacctcgta cttaatctgt ttttatctat cgactagaat ctgtatatat
    21601 tccagctatc aatgaggccg cagaaaaggg tgcttaaaaa taatctgttg tttaaaatat
    21661 tattatcaga atacttagat acaaaattct ggcacaaagt aaaaaaaata ttgacttaaa
    21721 aaaatggata tggtaatcta taaacgaaaa tagtggtaac gtttccaaaa tgcatcttcg
    21781 ttaaattttt atttttttaa acgaaaatgg aaacgtttct aaaatgtatt gggaggagat
    21841 tgaattggaa aagacattgg gaccaggtgg tgagataagg gcggtacaac gggataaaaa
    21901 aaatatttcg aagtgggaaa aattaaagaa acagttgcgg cgtgataagt ggatgtactt
    21961 gttgctctta ccaggtatca tcatgacgtt tatctttaag tatgtgccaa tgtacggtgc
    22021 tgtgattgca tttaaagact ataatcctat gctaggaatt ttggaaagtc cttgggtagg
    22081 actagatcac tttagggaat ttatcagttc accaaatttt acgcttttgc tgagcaacac
    22141 attaaaaatt agtatttatg gtttattgtt aggttttttt ccaccaatta ttttagcact
    22201 gagcttgaat caaattatca ataaaaaaat aaaacagaaa atccagttga ttctatatgc
    22261 gccgaatttt atctcagtag ttattattgt ggggatgctg tttttgttcc tatccgcaaa
    22321 tggacctatt aatgggttag tagaaaaggt tacggggcaa ccaattatgt tcatgtctaa
    22381 tccagcttat ttcagaacga tttatatttt atcgggaatt tggcaaggaa tcggctggtc
    22441 atccattctt tacaccgcaa cgctggcggg agtcagtacc gatttatatg aggcagcaaa
    22501 tatcgatggg gcaaatattt tacaaaagat ttggcatatc gatttgcctg cattgaggcc
    22561 tgtgatggtg atcagcttca tcttggccgc cggcggtatt atgaatgtag gatatgaaaa
    22621 agcctacttg atgcaaactt caatgaacat ccctgcctca gaaatcattt ccacttatgt
    22681 atacaaagtc ggcttgcaat caggtgatta tgcctactct gctgctgttg gtctgttcaa
    22741 tatgattatt aatgtcgttt tattactgat tgtcaacaat ataaccaagc ggctaaacga
    22801 tggtcaaggc ttgtaatgaa aggagaaaat tatgaatagt gctttatcaa aaatcgaccg
    22861 tcaaatctta tggttgaatc gagtgattgt aactttttta gtcgtcatca ccattgttcc
    22921 gctgatttat gtactggtag cttcttttat ggacccagct actttgatca ataaaggtat
    22981 aagcttcaat ccgaaagatt ggacaataca gggatatcaa cgggtatttg cagatgattc
    23041 cattttacgc ggttttgtaa attcgttgtt ttactctttt agcttcagct tattgactgt
    23101 atttattacg atgatcactg cgtatccttt gtctcgacct aacttggcgg ggaaaaagat
    23161 tattatgact ttttttgtca ttacaatgtt cgtgggtggt ggcttgatac ctacctattt
    23221 attggtaaag aacttgggca tgttagatag tgtatgggcg attatcgtac caggtgctat
    23281 aaatgtgtgg aatattatct tagcccgtac ctattttagt gctttgccaa aagaactttc
    23341 tgaagctgct actatcgatg gcgccaatga cttgcaaata ctgttcaaag tcatgcttcc
    23401 attagcgaaa ccaattatgt tcgtattgtt tttatatgcc tttgttgggc aatggaactc
    23461 atactttgat gccatgattt acatcaaaga tcccgactta cagccgttgc aactcgtatt
    23521 aagaaatatt ttgatccaaa accaaccttc acaagacatg gtgggagcca atactgccat
    23581 ggcagaaatg aaacaaattg ctgagttgat caaatacgca acgatcgtca tttctagtct
    23641 gccattaatc ataatgtatc ctttcttcca aaaatatttt gataaaggtg ttttagttgg
    23701 ttctgttaaa gggtaagtat tgttcactat ttgtatgaat aaaaattata aaggagattt
    23761 taagatgaaa gcaaaatgga tgctgccaat cgtttttact tccgtactaa ttttgggggc
    23821 ttgtggaggc tcaaaagaaa agagtaaatc aagccctgat tatgaactag aaaatgtctc
    23881 ttttccattg aaagaaaaag tatctttaaa aatgatgtct caaagtgccc ctttggctcc
    23941 caatgatcct aatgacaaat tgattttcca acgattagaa gaagagacca atctccatat
    24001 tgattggaca aactacagta gtgattttgc cgaaaaaaga aatttggata tcgctagtgg
    24061 tgatttacca gatgccatct tcaacgccgc tgcaggtgac tatgatttat taaactgggc
    24121 ggaaagtggc gttattgtac cgattgaaga tttgatcgaa gattatatgc caaatctgaa
    24181 gaaaattttt gatgaaaacc ccgaataccg acaaatgtct actgcacctg atggtcacat
    24241 ttactctatg ccttggattg aagaattagg cgaaggaaaa gagtcgattc atacagtaaa
    24301 tgggatggct tggatcaaca aggagtggtt ggataatttg ggtcttgaaa tgccgcaaac
    24361 cactgatcag ttgatgactg tgttggaggc tttcaagacg caagatccaa atggtaatgg
    24421 tgaagcagat gaaattcctt ttagctttat taacaatgga ggaaacgaag acttaaaatt
    24481 tttaatgggg gcattcggtc taggtgataa tgatgaccac ttagtcgttg ataacgatgg
    24541 tcaggtacaa tttactgcta acaaagaagg gtataaagaa gccatcaaat atttcaatga
    24601 aatgaataat aaaggtctta ttgataaaga ggctttcgaa caagattgga atacgtatgt
    24661 tgccaaaggg aaagaacata agtatggcgt gtactttact tgggataaag caaatatcac
    24721 gggtgcagat gatagttatg acgtcttgcc agtcttagcg ggaccagatg gaacgaaaca
    24781 tgttactcgt acgaatggca tgggtttctc aagagatcgt ttcgtaatta ctagtgcgaa
    24841 taaaaacttg gaacttacag ctaggtggat agataaaatg tatgaaccgt tacagtctgt
    24901 ccaaaataac tggggaacct acggtgatga agcacaggcg aatatttttg aattaaagga
    24961 tggtatgtta aaacatttgc ctttagaggg gacagcaccg ggggaactaa gacaaaaaac
    25021 cgaagctgga ggaccattag cagttcttga ttcttattac ggcacagtca ctactatgcc
    25081 agatgatgcg aagtggcgtt tagatttgat gcatgacacg tatttgccat atattacaga
    25141 agacaatatc tatccacgtg tctttttaga aaaagaagaa acagatcgat tagcaaaaat
    25201 cgaaacggat atgtgggatt acatctatcg caaaagagca gagtggatca ccggcggtaa
    25261 aatcgatgaa gaatgggaag aataccttgc tgaattggaa cgtgtaggtt tggaagaatg
    25321 gattcaaata aaacaaactg gctatgataa ttttttgaag ggagcacaat aagtgttagt
    25381 agaagaaacg gcgattttgg ataaagcaaa tcgatttatt gaaatgatgc gaggaaaagt
    25441 taaccctcag tatcgacaaa actatcattt ttccgcaccc atcgggtgga taaatgaccc
    25501 caatgggttt atatattaca aagggcatta ccatctcttc tatcaatact atccttacga
    25561 tagtcgatgg gggcccatgc attgggggca tgcgcgcagt agtgatttgg tccattggga
    25621 agatctacca gtagcgttag ccccagatca accttatgac agcggcgggt gtttctcagg
    25681 aagtgcaatt gaaagagacg gaaaactata tttattttat acaggctgtc aagaaattga
    25741 tggtattagc catcagcatc aatgtttggc ggtctctgaa gatggtattc attttagcaa
    25801 atacagtggt aatcctgtgg tagcagaaaa acagctaggt gaaaatgggc taatacaaga
    25861 atttcgtgat cctaaagtaa ttgttcacaa cgatggttac tatatggtag tagctactaa
    25921 atctacacag tctactggta gaatagtgat gtttcaatcg tcagatttgt atacttggca
    25981 cttttactct gtgctgctag aagggacttc agagcaagga ataatgtggg aatgtcctga
    26041 tttattccat ttagacggac gagatgtctt aatcatgtct ccgatacaaa tcaagaagaa
    26101 cggactggaa tatcataata tcagttctac catggcttgt attggtgagg taaactggga
    26161 aacaggtcag atgatagttg ataatttcca tgaaatggat tttggaatgg atttttatgc
    26221 accacaaaca acaagagatc ctaatggtgg acgggtgatg attgcttgga tgcagatgtg
    26281 ggatcgtaca ttgcctacac atgagttagg ccatctttgg gcaggctcta tgagtttgcc
    26341 ccgagagtta agagtgaaaa aaaatcgatt ggtacaaaaa cccatctcaa ctgtatatag
    26401 tcatttagtt tatcaacaag gcagtgaaaa tattgcagtg actgacacat cagtcgtatt
    26461 tcgaaacgcg ctgtctgatt cttcatattt acatctagtc gcagatttgg agaaagctga
    26521 agtttttaca attgaattac tgcggtctaa gacggattgt ctcaaactag cctatgacac
    26581 tgctacacag gtttttgaat tggatcgtca gaaactagga agagagctta caggggcgga
    26641 agaagaccct ctgaatgtac gaaaagttga agtacctttg attgataatc agctcgttct
    26701 tgagattttt agagatacct catcaattga agtctttatt aatgggttag aagttatgac
    26761 ggctactttc tatgagctgg aaaaagctcg agatatcttg tttcattcga tcggagaaag
    26821 tcgtattcaa gcttttgaaa cagcgagtgt gaaataaatg tttgattgct taggcgatga
    26881 atcaattatt taataggttt tgaaggataa tgtttttaga aaagaacaga tagctagatt
    26941 tcccatggat ttgctattag tattttgaaa aaattagtaa tttatgacaa aattcattat
    27001 actaaacttg tgcagaaatg tgagggatca actatgaaag caacaatgaa agatgtcgca
    27061 aaattagcag gtgttggagt aggaactgtt tcccgggtat tgaataacgg ttctgttaaa
    27121 gcttctacta gagaaaaagt tgaagcagca atgacagagt taaactatca accagatttt
    27181 tatgcccggg ggttaaagat gaagcatacg aacacggttg ctttgattct acccacggta
    27241 tggcatccat tttttggcga atttgcctac catgtagaaa cagcattaga aagacaaaac
    27301 tacaagcttt atttgtgtaa ctccagtggt aatagtgaaa aagaacaaga atatatccag
    27361 atggtcagcc aaaataaagt agacggcatc attggaatta cttattcaga tatcgatgaa
    27421 tacatttcga gtaatctgcc ttttgttagt attgaccgtt atttcactga agaggtgatt
    27481 tatgtgaccg ctgacaatgc ggccggtgga cgtttggctg caaatgaact ttatcgccga
    27541 ggatgtcaga atgtagcata tatcggtggg taccaagaaa caccgaatga aacgaagagt
    27601 cgtcgaaaat attttgaaat cgagtgccgt caactgggga tttcctataa agcgttagat
    27661 atgttagaac ctttagacca tctctcagaa cgtgtaacaa attttttgtt agagaatccc
    27721 gatatcgatg ggatttttac gatcaacgat ccaatggcat tagctgtcat tgatatgctt
    27781 gaaaaaatag ggaaaaacgt accagaggat gtccaagtta tcggatttga tggtcaaaag
    27841 atgacagaag gagatcctta tctagtctca acgattgccc aacctacaaa gcaaatggcg
    27901 gaagtggcag tggcaaaatt aattgctgtc attaatggag agacagtaga aaaacgtacc
    27961 gtattgccga ttgaatttgg cgaaggtgac acaacgaaac ctttagtgag aaacgatttt
    28021 gcttagtgca aatacgtttc tttttttgaa aatgagtgaa tgaaaggtgt agataaaatg
    28081 ttatatggtg gaatagaagc cggtggaaca aagtttgtct gtgggattgg taatgaacaa
    28141 ttacaagtga tagaacggaa aagttttcct accacaacac cagaagaaac gatggagcaa
    28201 gtgtttgatt tctttgataa gtacaaagga aatatcgcat caattggtgt aggagcattt
    28261 gggcctattg atattcaaat cgaatcaaga acttacggat atatcaccaa cacaccgaag
    28321 ttagcttggc aaaattatca ttttgtagga gctttggaaa acgccttgtc ggttcccatc
    28381 tattggacta ccgatgtaaa tgctgcttgt tatggagaat atatggcagg gtatggaaaa
    28441 ggtaaaaata gtgttctgta ttacaccgtt ggtacaggtg ttggtggtgg tgctgtagtg
    28501 gatgggaaaa tagtcgcagg ttttagtcat cctgaaatgg gacatatgct cttacaacgt
    28561 cataatgaag atcaatttga aggaatctgt ccttttcatc atgattgttt ggaagggtta
    28621 gcatcaggac ctgcgattga aaaacgcttt ggaagaaaag cccagactat ctcatttgat
    28681 catccttatt ggacgattga ggcagattat cttgctcagt gtgtgtataa cacaacgtta
    28741 ctatatgcac cggaagtaat aattttaggc gggggtgtaa tgaaacagcc gcaactactc
    28801 gagttgattc gccaatcgtt taaagaaaaa atgggttcct acgtggatac gcctactctc
    28861 gaagaatatc tactaactcc aaggcttgct gacaatgcgg gcttgattgg ctgcctagct
    28921 ttagcaataa agaaactcaa tcattagaat agtatttatt agaactattc ataacatacc
    28981 ggaagtttaa aagggtattc tgaaacttaa atagtctcaa ttcattcttc tttcttgaga
    29041 atctgagtag agacttttat tctgtttcaa attaatataa ttacgaaa
//
LOCUS       DAYYME010000023        28701 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000023 DAYYME010000000
VERSION     DAYYME010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.023"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACHBFW_000828"
     CDS             complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACHBFW_000828"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFM9317162.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACHBFW_000829"
     CDS             complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACHBFW_000829"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFM9317163.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACHBFW_000830"
     CDS             complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACHBFW_000830"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFM9317164.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2179..2499
                     /locus_tag="ACHBFW_000831"
     CDS             2179..2499
                     /locus_tag="ACHBFW_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFM9317165.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2611..3468)
                     /locus_tag="ACHBFW_000832"
     CDS             complement(2611..3468)
                     /locus_tag="ACHBFW_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM9317166.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(3576..4880)
                     /locus_tag="ACHBFW_000833"
     CDS             complement(3576..4880)
                     /locus_tag="ACHBFW_000833"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9317167.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4877..5386)
                     /locus_tag="ACHBFW_000834"
     CDS             complement(4877..5386)
                     /locus_tag="ACHBFW_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317168.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5561..7696)
                     /locus_tag="ACHBFW_000835"
     CDS             complement(5561..7696)
                     /locus_tag="ACHBFW_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM9317169.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7779..9434)
                     /locus_tag="ACHBFW_000836"
     CDS             complement(7779..9434)
                     /locus_tag="ACHBFW_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9317170.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9436..9750)
                     /locus_tag="ACHBFW_000837"
     CDS             complement(9436..9750)
                     /locus_tag="ACHBFW_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM9317171.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9920..10180
                     /locus_tag="ACHBFW_000838"
     CDS             9920..10180
                     /locus_tag="ACHBFW_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFM9317172.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10194..10508
                     /locus_tag="ACHBFW_000839"
     CDS             10194..10508
                     /locus_tag="ACHBFW_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFM9317173.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11238..11792)
                     /locus_tag="ACHBFW_000840"
     CDS             complement(11238..11792)
                     /locus_tag="ACHBFW_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFM9317174.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11918..13847)
                     /locus_tag="ACHBFW_000841"
                     /pseudo
     CDS             complement(11918..13847)
                     /locus_tag="ACHBFW_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(14153..14869)
                     /locus_tag="ACHBFW_000842"
     CDS             complement(14153..14869)
                     /locus_tag="ACHBFW_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM9317175.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACHBFW_000843"
     CDS             complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACHBFW_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFM9317176.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACHBFW_000844"
     CDS             complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACHBFW_000844"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFM9317177.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18339..19799)
                     /locus_tag="ACHBFW_000845"
     CDS             complement(18339..19799)
                     /locus_tag="ACHBFW_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9317178.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19789..21084)
                     /locus_tag="ACHBFW_000846"
     CDS             complement(19789..21084)
                     /locus_tag="ACHBFW_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9317179.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21416..22093)
                     /locus_tag="ACHBFW_000847"
     CDS             complement(21416..22093)
                     /locus_tag="ACHBFW_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFM9317180.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22123..23214)
                     /locus_tag="ACHBFW_000848"
     CDS             complement(22123..23214)
                     /locus_tag="ACHBFW_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFM9317181.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(23315..23749)
                     /locus_tag="ACHBFW_000849"
     CDS             complement(23315..23749)
                     /locus_tag="ACHBFW_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM9317182.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24333..25790)
                     /locus_tag="ACHBFW_000850"
     CDS             complement(24333..25790)
                     /locus_tag="ACHBFW_000850"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9317183.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25787..27682)
                     /locus_tag="ACHBFW_000851"
     CDS             complement(25787..27682)
                     /locus_tag="ACHBFW_000851"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM9317184.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27740..>28701)
                     /locus_tag="ACHBFW_000852"
     CDS             complement(27740..>28701)
                     /locus_tag="ACHBFW_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM9317185.1"
                     /translation="QAIENRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSL
                     LFHETEFLLTHIKQMKTRMDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNL
                     AESAYFVLYLAEALNPFRKQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVY
                     LFKNQSNKITEYDAILTTEQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEK
                     KKTKLLDHYYKEENQIKIHKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKI
                     TEYQLEIPLIHQQRKIKRVFFVSFDYKRNDILVFFQAVSDLLG"
BASE COUNT         8021 a         6223 c         4883 g         9574 t
ORIGIN      
        1 ttttattgaa cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta
       61 gaagcagctc ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct
      121 tttttagcta ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa
      181 ttccctgcct tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca
      241 gcttctgcct ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat
      301 ttttcttgag aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca
      361 gatccgtata acggaccaga agccccaccg actttcgata tcatcgccat agagaatact
      421 ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa
      481 acccctcttc ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg
      541 agatattgtt tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct
      601 tgaacggtta gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc
      661 tgtacattgc tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga
      721 gaaagacctt gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg
      781 acgcctcttt cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa
      841 ggcgtcccgc ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca
      901 tctaaaatct tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga
      961 cgatagcctg gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg
     1021 aatcctggtt tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta
     1081 gttgcctgta cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt
     1141 gctgcatcgc ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca
     1201 gtatacgtac tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata
     1261 tcgtccatag cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc
     1321 ttaacgatca gcaaaactcc ttttccacta tcggcttcct taatagccgt ctgaatctga
     1381 tctggagttg gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct
     1441 acaaacccgg catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt
     1501 tccgctttct gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga
     1561 tagcttttta ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt
     1621 tttttcaatt tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg
     1681 agtatcatct gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc
     1741 atccaccaga tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga
     1801 gttcaatacc gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc
     1861 attgactgct tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag
     1921 agaaaaaatt tgcacctctt cggatttttg catctgctca atcatttctt taataccatc
     1981 tgttactttt tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc
     2041 ttttacatag ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat
     2101 aaatttctaa ttttgggaaa atatgtgtag tgcttcttga agtttgttat actttaatta
     2161 aaagagagga gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta
     2221 gtagcttata aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac
     2281 ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta
     2341 atgggtgcaa gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct
     2401 gatgcgatca ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg
     2461 atggtcacat gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat
     2521 aactaaagag agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc
     2581 gaggtactct accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc
     2641 tgctgcgact aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt
     2701 tgtttttggc aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc
     2761 tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg
     2821 ttggaaacta gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc
     2881 ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact
     2941 tagctttcca gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg
     3001 ttggatcttc ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag
     3061 ctttttctca agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg
     3121 atcgattgct gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag
     3181 ttcttttctt ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact
     3241 atttattgat gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt
     3301 ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca gaagagctta tggttgttga
     3361 aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat
     3421 actggaaaca gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat
     3481 ccttactact ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac
     3541 acaacaggct gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt
     3601 ttccatgtcc cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg
     3661 atcgttatcg cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat
     3721 ttcaattttc ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg
     3781 accatttcaa agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag
     3841 tttccttgat atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag
     3901 aatattgacc agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt
     3961 ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt
     4021 gcccaacgtt ttctgtgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag
     4081 ataatctcag gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg
     4141 atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga
     4201 tacatcgtat ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt
     4261 ttaatgatgc ccacgatact tgagaattca accgtttgag attttgcaat caatttggta
     4321 cgattttgga catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg
     4381 aaataattca catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt
     4441 aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta
     4501 tttttttcga tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt
     4561 tctggtgttt ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc
     4621 aatttcgtca tcaaatactc gattgtatat tcgatcacaa tttcttcatt atgggctggc
     4681 accatgatcg tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta
     4741 tgtttgtata aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc
     4801 ggataagaaa cgagaaaaaa atacataata gacaaacaag tatctaagaa taaattacct
     4861 gtatgggtga ttgagatcat ttcagttcca ccccattttc tttatacaaa tctgcaatga
     4921 atgtcgtatc tagatttttc tcttcggaga cagaataaaa acggaccgtt tctctctctt
     4981 cttttgttcc aaaacgttcc gtatacactt cttcaagtaa ttcttgtctt tttttcaatt
     5041 tttcatcttc atattcaaaa gatttattta cttttttgta tagatttcga ttgttccacc
     5101 tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat caatacaaaa aagccgatta
     5161 agaaatacag cattttcttt tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt
     5221 ctggtctcgt taatgggaaa aagacccata taaaaggaag aacaacgatc atccatccta
     5281 aaagagccac taacgtttgc caaatcttta accagtaatt accttttgaa aaatacttat
     5341 ctttcaccaa aattgatgca acttcttctc ttgttacttt tttcatatcc tcacctcacg
     5401 tttttttgtt acgacgtagg agtttaccat acatttcatt tcttttcaca taaagtgttc
     5461 ctaaattttt cctaagaaaa agaacaaaac atttctcatc agcaaatgaa aaatattttg
     5521 ttctttttat cagttgttct ttttattttt tatctcttaa ttatgttgct ctttccaata
     5581 cgacaatgct tttcattcct ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct
     5641 catctccatc gttttcaaat ggatataaga ttacatgatc aaattccagt ccttttgcta
     5701 gactcaaagg aatgatttgg cagttttcta atccatatga atttttttta atcgattctt
     5761 gtagcgtttg ggtttcctct tctgtttggg tgataactgc cgctttttct ccttttagag
     5821 aaaccaaaat ctcagctagt attttcaaca attcttcaga ggatcgaaaa gagcggtatt
     5881 cgggttcttt tccttgcggt cggatcgata cgatgtcgat cttctcttga tccacagcat
     5941 aggttttgaa aagctctgtg attgctccgc tagaacggta actatttcta agatcatacc
     6001 gatggatcgg cagatggtag tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag
     6061 aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt
     6121 cgatcaatag acctaattgt gccagggtat aatcttgtac ttcatcaatc aatagatagc
     6181 gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg aatagctaat ataatgactg
     6241 cttcatcaac agtacgtgtg ggttgccaag cataagctga gccatatcgt ttttcataaa
     6301 ttctttcaaa caactgatgc tcatccaccc atgccatttc ttcgatttgt ctggttattt
     6361 ttcgatattt tttttgtaaa agtttccttg cataagctac aatactttgt tccgaatcat
     6421 cagaaatcaa tttaccgaat aattccaact gcctatcttc tgtcaacgag agtacctgat
     6481 tctgcacagc aggacttttt gcttgtttca aaagatgatt cttccattcg ctcatcagtg
     6541 cctttttcgt tgcttgaagc ctatcgatca atttaggatg agcaggaact ttctcatata
     6601 ttttttcgat cgttttttta gaaaggactg tttttccatt tttagtaagt ccacggataa
     6661 aattcggatg atctaaaaac aaaggatctg attgttttag attatcgata aactttttgc
     6721 tacgcaaacg ttctgtctgc tccgatacat tttctcccgt gatacgtttg aaataagcct
     6781 cttcaccttc tatttcctct gctgaaagct gtgaaaccag ctgtatgatg gttagattca
     6841 gtggatttct ttcacctaat gatggtagga cattcgaaat atagtcgata aaccgatgat
     6901 taggtgatag aatcagcaag tcatcggatg tcatcgtatc tcgatataaa tagagcagat
     6961 acgcaatacg ctgcatgata gcagaggttt taccacttcc tgctacgcca tttaccaaga
     7021 tattttgact tttcacatcc cgaatgattg tgttttgttc cgattggatc gtagcagtga
     7081 tagccttcat ttcattggtc tcatcttggc ctaagacttc taataatacg tcatcttgaa
     7141 tagctacaga cgtatcaaaa aacttgacca aacgatctct ttccaaaaga aattgtcgtc
     7201 tttctttaat aaccgtatcg attttatttt gacgaaccgt ataagaagat ggaccaatca
     7261 tgttattgta aaaaagttcg gcaattggtg aacgccaatc ataaacaaga ttttcatctg
     7321 catcatctgt gaaatttgct gtaccgatat aaaaggattc ttttggctca ttatccagaa
     7381 agtctacaac gacttttcca aagtagggac ttttcaataa acgttcgatc tttgctagct
     7441 gattttctgc gtttttctgt ttgatattca tctgatcgat ttctctattt ttcatttcaa
     7501 tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa attcaatcga agctcgccag
     7561 ccatttcttt caacgactgg tttccttcta tttttgccgt atccatcgat tcccgcagtt
     7621 cgtttttttt atcaattagc ttttgataca cgttcttcag atatgcttct tctaactgtt
     7681 tttgtgtgtt gaccatacac attctcctct ttttttaaag tggtggaacc gattagttta
     7741 cctcttttaa gagtcgaatg caagcttttt aacgagtatc atttgttagg atatatcagt
     7801 tcttcaggtc ggacagtttg atataaagaa aatgcaccat caaggttttt tacgtggtag
     7861 ccagactgct tcaagattct ttccgctaga tagctacgta atccgctatg acagctgacg
     7921 atatattctt gttggctgtc taattcattt aatcggtccc gaagttcatt caaagggata
     7981 tgtttggctt ccttgaattt gcccttttcc aattcttctg ctgttcgcac atctaataaa
     8041 atttttcctt ttgctagttc tgtcggcaat tcatgccatt gtacggtttc actaagccct
     8101 tctgcaatgt tcattgctgc atatcctagc atgtttactg gatctttggc tgagccaaat
     8161 ggcggtgcat acgtcaattc taattccggt aaatcgaaga tcgtcaaatg ccctttgatt
     8221 gctgttgcta aaatatcgat ccgtttatcc acaccttttg ccccaacacc ttgtgcacca
     8281 taaatctcgc cagtctctgg atggaagatc aacttcagta aaatatctgt tgcttctgga
     8341 taatagcttg catgatcttt tcctgataca tgaatgactt caaaagacag ttcgttttct
     8401 ttggctgttc tttcacttaa accggtggat gcggcagtca ttccaaacgt tcgtataatt
     8461 gctgttccaa taccgccttg atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt
     8521 ctaccttgac gattggctgg tgaagcaaga gaaataagtg catcttttcc agtgatttgc
     8581 tgttttacaa cgatagcatc ccccaccgca taaatatcag gaatattcgt ttgataacgt
     8641 tcgtctacta ggataccccc gcgtatgcct aattcgattc cagcggtcac tgctagctgg
     8701 ttatcaggac gaacaccaat ggataaaata actaaatcag cagggatggt acgcccatca
     8761 tccagtcgca atcgtttccc gtggtcttca aatccagcga cagcatgaga agtaatcact
     8821 tctactcctt gatgagacaa ttctttttct ataaaagctg ccatttcttc atccagtgtt
     8881 ggaagaatat gtggtgcttg ttcgatcacc atgacttcta aacctcttct ttttaggttt
     8941 tctgccattt ccaatccgat gaatcctgca ccaacgatca cggcacgctt tggctgtttt
     9001 tctaatgcat gcatcacttt atcgatatct ggtacatttc taatagaaaa aacattgtcg
     9061 gcttctgtta tccctggaat cgatggaaca actggttttg caccaggaga taaaaccaat
     9121 gcatcgtact gttcaatgtg tttttgaccc tcatgtacga cttctaccgt tttgttttcg
     9181 ggaaagatgg ctgtcacttc atgatttgga cgcacatcta aacaaaaacg agcggataat
     9241 gattctggcg tttggacaag aagattttcc cgttcactga tttctcccga aagataataa
     9301 ggcaagccgc aatttgcaaa agaaacatac ggtccttttt caaaaacaac gatttctgca
     9361 tcttccatta atcggcgaag ccgtgtagca gcagacattc cacctgctac acctccgaca
     9421 ataacgattt tcatcttatt tccctcctac tgttcgatca aaccattgat tcatgccacc
     9481 acgtacattc actacatcat aaccagattt ctttaattct ttagctgctt gtttactcct
     9541 catccctgat tgacaaatca catagacggt cttttctttg tctccatcat atctgtttat
     9601 actttgtaat ggaacatttt tagctccttt tatatgcccg ccccggtatt ctgcaggtgt
     9661 tctaacatca agtaacgctg gattttccaa aagcttttcc tttaattctg ggactgttat
     9721 cgattttatt gattgaaata gcaaattcat tttctgcacc tcatttacat ttttataaat
     9781 acctcatggg gtatattaaa caggagcttt attaatatgt caaccagaat gactcttcat
     9841 ttttttcact actttttata tttgcataaa ataccgataa ggggtattat ctaattataa
     9901 aaatagagga gaatgtgtta tggagattga taataaaaat atcctgaacc gtttgaaacg
     9961 tacagaggga caaattcgtg gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga
    10021 tgtcatcact caattaagcg ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt
    10081 tgcagataat ctgaaaaatt gctttgagaa acctgatagt aatccagaag aacaagcggc
    10141 taaactagaa caagcgatca agatgattat taaaaagtaa aggaatgatt tcaatgaaag
    10201 tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc
    10261 tgataaaagc ttctccagaa acgacgattg tcgtatctgt aaatggcatc gcgattaccg
    10321 gttatctaga ctctgccaat gcagaattct tagatttgca aggagtcgtt tttcacgctt
    10381 gcgccaatgc tatgcgtgct aatcatattt cagaaagttc tttacctgaa caagtaatcg
    10441 ttgtaccagc aggtgtcctt gatttagttg aactgcaatc ccaaggttat gcttatatca
    10501 agccttagtc agtagaggtt gcgacagaag tgatcagctt caagaaattt cggcttattt
    10561 ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat
    10621 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttgggctta
    10681 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt
    10741 gacctaagta ttagagaaaa aaacgggaaa atagctcctc atattttgcc gttttttgag
    10801 ctaataccga aggtcagctg attgaacacc gtttaaggaa gagattgtga aaaagtcgct
    10861 tagtttcgag tattaagaaa aattttgtga aaatggattt ctacattttt gcataaattt
    10921 ggcttaatac cgagaaactg gcttttgaat accgtttaag gaagaggttg taaaatcagc
    10981 ggtttgacct gagtattaga gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt
    11041 ttgagctaat accgaaggtc agctgattga acaccgttta ccaagaggtt gtgagcctac
    11101 catttaactc cgaatcacaa gaaataattt caaaaacaac ttctcctgtt tttggttcgg
    11161 ttggcttatt atcgagaagt agctactggg agtctattta tcaaaaaaag gaatgcgaca
    11221 taggtcacac ccctttttta ttttctttga aacatctttc ggtactcgac agcatacaag
    11281 acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt
    11341 ggtaattctg ggaaaagcag caaaaggatc atgccaacat agatgacagc agtcgctact
    11401 ttgccatacc acttcgctcc acttaatttg atatttttag atagcccgat taatccagct
    11461 acaaacataa agccttcttt tatcaagaat agaccaaaca acagccaaag ccacggtcga
    11521 taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc
    11581 accttcccta agtctgttac ctgattatat ctccgagcaa tgaccccgtc tagataatca
    11641 gacaaacctg agacaacaat gaccgctcct gctatataaa atgactgtct cagcacaaaa
    11701 taaagataaa tcggcagcaa aaaaatacgg aatatcgata aaagattcgg aatcgtttgt
    11761 atttctttac gccagtctgt gcttaatttc attttatcgc cctacctcct cagcactctt
    11821 ttctttttat tttagcttac acggggaata aaaccaatga tttcactgtt tttatttttc
    11881 tttacagaca ctgatattat gagctaattt gagataatca cttgctgttt tcatcgttta
    11941 ctcttctttc ttcctgtcgt ttgcgcattt cttttactag tactttatga gctttttttg
    12001 atcctcgctc taggtcacgc tgccgtttac gtgttttata atcgataaac agactgtcta
    12061 aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac cagacgaaca cgctgacgag
    12121 gcacgtggat tatttttttg tctaaatata catcaatcgt atcttcacct atatcttcat
    12181 aaactaacgc ttctttttgg tattcattga caaatatccg atcacctttt gcaaagagct
    12241 gacgttcttt tttattctcc ttcctatttg taactgctgt cttaggaaga aattcttttt
    12301 tctgtgtccg ataggctttt tccgccaagt aacgctctgc ttgatggatc aatttcatcg
    12361 acatatccat tttatgagca atccacaaag cttggctttc tcctgtttct tccaacccgc
    12421 aactgatatt taggacgaag tgcttcccga tcgaaagcca tagcagccgg cacaaaatct
    12481 tcatgatctc cggcgaattt cttgatttct ccataatggg tcgtggcaat gatcaatgcc
    12541 ccttgctcat acatagattc catgattgcg atcgctagtg cagccccttc atttggctct
    12601 gttccactgc caatctcatc taaaagtacc aacgtattcc tttttatctt tttcaaaata
    12661 gcagcgatgt tttgcatatg accagaaaat gtactcaacg cattttctaa attttggtgg
    12721 tcaccgacat cgacgaaaat ctcatcaaag acagcaagct ctgtcccttc ttttgccgga
    12781 acttgcatac cgaacatcgc catcaaagtc aataaaccga ctgtcttcaa cacgactgtt
    12841 ttgcctccgg catttgctcc ggtgataatc aagcctcgat agtctttacc gatttcaaca
    12901 tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt
    12961 tccgatttat tgattctagg tgtgcttcca ttgatttctc ggctaaattt tgcccgagca
    13021 aagatgatat ctaacaccgt gatcgtttcg atcaataaat cgatcacttc ttcgttttct
    13081 gcgatagcac cagtcaatgc cgccaggact tgatattctt cactgatttc ttcagctttt
    13141 aacaactgat agtgttcatt caatttagaa atcacagttg gttcaataaa aaccgtcgtt
    13201 cttttatttg attcatcgat aatcgttcca tctattttat ttttatagct ggctttgata
    13261 ggaatcgtat agtattcgcc tttttgaaca atcattgctt cttggatcat ttctttgttt
    13321 ttcggatgac gcaagaattt cagtaatttt gactggattt ctttttcgat tagttgtaat
    13381 tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac tgactttatt attttttatt
    13441 ttctggtcaa tcaactcttc tacatttatc aaatcaggaa gatgtttgct ataagcaaac
    13501 aacaagggtg tctggtattg attcttatca aaaaactttg tgatcatccg actgcttcta
    13561 agaaaatccg catattcgat caaatcggtt ggctgcagaa tcaacccttt tttgacttgt
    13621 tctgtcagtg catcgatcct cggcaacccc ataaatggaa catgctgact gctttctaag
    13681 atcaagcgtg cttctcttgt ttcttcttga cgatccaaga cagtttgtag gtttgtcgag
    13741 accgtcatat cgctgattcg ttttttgcta tagtttccaa ttgctcttgc ttggatttcc
    13801 tttttgatcc gatcaaattg taattgacta atcatttcgt tgtccatatt ctctcctttt
    13861 cttgaagaga tacctgtccc aaaataaata agggcctaga gataaataaa aggatagaac
    13921 acagcttaaa aaagtcatgt cctatcctta ttagaataca aagatattgt tttcctgaca
    13981 ggtaccgttt cataagcaca ccaaataaac tttgtttatt ggctattcga cttactgatt
    14041 agataccagc tacactcaca aaacataccc cattcttcta ggtcttattt attttccact
    14101 taactatagc aagacacatt ttggttgtca aactttagta tgttttatag atttacacca
    14161 ttaatgaata gacacctacc gcgataattg ctccgatcat tggacctgcg attggtaccc
    14221 atgaatatgc ccaatctgaa gttcctttat tagctattgg caataccgca tgtgctaatc
    14281 gaggaccaaa gtcccgagca gggttgattg catatcctgt tggccctcct aatgaaagac
    14341 cgatggctaa gatcaatcca cctacagcga atacgttcgt tccaggtgca aattcgtttt
    14401 gtccaaaagc tagcaaccca agaactaata caaatgtacc aattaattct gtgatcacat
    14461 ttgctggata attccgtata gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat
    14521 ctttcgttgc attccaatgt ggcaaataag aaagccaaac aagaatggcc cctgcaaaag
    14581 ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt ccaactaatt gcccctgtca
    14641 tcgccatagc tagagagact gcaggattca aatgagctgg gcccatgaat ccagaaatat
    14701 agacagccat tgttacagcg agtccccaac caaatgcgat gacaatccag ccagaagcct
    14761 gtgctttact tttgtttaaa ttcacagccg cacacacacc atctccaagc aaaactagga
    14821 tcagtgtccc aataaactcc cccatgattt gagtcatatc actgttcatt tatttctccc
    14881 cctttagttc ccgcagatct gattctgcaa tcaatgcttc taaacgttta gtctgttgcg
    14941 ccttctcctc ttctgtccat tcaaaatatc cagcaattgc atcgatcaca ggttgcttga
    15001 tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt acgacgaatg agataatcgc
    15061 ctggggccaa aaccatttct tcttctaaac cataacgtaa ccgagctgac tcagcgagac
    15121 tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa gatacgtcgg gcattagtcc
    15181 cgtaaaaatc agcgatataa gtagcatctt cttcctctaa accggctgct actccttctt
    15241 ttgctaattc tgtcactgtt tcttctaatt tagttggatc gaagtttcca cctgaaatct
    15301 gatatttttt agagtcgatt tctttcgtct ctattccgta ttcttctttt aataattggc
    15361 gtatcaatcg caaagctcct tctgccatct tacggtaatc ggtgattttc ccaccagata
    15421 aagtcagcaa accatctggt tctctttcta aagaactacc tctggaaatc gtagaaggtg
    15481 ctttttcatc acggctgttt tccaaatggt tcaacacgtc ttctacttca gcacgagaaa
    15541 ctttgttttc cttatattca cttactgtat cgacaacttt attgaagctc ttgtctgaaa
    15601 tcgatccatt atctccacca ttgtaatcag aaccagaatt accaatcaac agtggacgaa
    15661 gtcctgccca gcttgcttcg atatcattca atgtgatatt tgcttctgga tagcgatgat
    15721 tgatcacatc caatagataa tctacatctt cttgtgtgac tttcggatcc gtaaaatctc
    15781 cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa
    15841 ccatccgccc atcttgcttt cctgtatcga agtatgtggg ttgcggtaca ggcagttttt
    15901 tcgcatctac aactaaatgg atccctttag ttggacgcat tttaggagag actggacgcg
    15961 taaaattcaa gtttcgtact ttatctaccc aaggaccact cgtattgatc actaattttg
    16021 ctttgatttc gatcacttca tctgtcagca ggtcacgagc tttgacgcca acgatttgat
    16081 cgccatcata taaaaagcca accgctttca ttttacttac tagataagcc ccatcttcgg
    16141 cagccttttt gatattatca atcactaaac gggcatcatt gttgcggaaa tctagataga
    16201 cacccgcgcc ttttagccct tcttttttca aaaacggttc tctttccaat acttcttctg
    16261 gtgtcagcgt ataattctca tatttggttc ctgtcacatt tgccagtttg tcataaaggt
    16321 ccattgctac tttgaccgag aacatattga aggttgttgc tccttcgccc tcatagattg
    16381 gcaaaagcat tggatctggt tttgggatgt gtggggcaat actttgtacg actgcacgtt
    16441 caccaactgt gtcagctact acttccacat caaatgtctt cagataacga ataccgccat
    16501 gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc
    16561 ctgttttgat tcctgatgct gctgactgga ctgccacacc ggcaccagtg attccgccac
    16621 cgatgatcaa cagatccaat tcttcttgct gcattttttg aatgctatct tgtcttgtct
    16681 tgtttgaaaa catatggatt tcctccttat tcaaatgctt gggtagcttt tactgctttt
    16741 ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca tttgaacttc aaatagcttg
    16801 ccagcttcat aaaattcgcg gatctcgtct gtatctttcc aataaccgac agctaatcct
    16861 gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca
    16921 ttcaagatat ctgtttggaa ttgcatcaga tattcattgt ttgctgcacc gccatctact
    16981 ttcaatacag gaatcttgat tccagtgtca tcttgcatcg tatcgatgat atctctcact
    17041 tgataagcaa tcgactgtaa agtagcttta atgatatctt cttttgtcgt accacgtgtc
    17101 aagccaaaca tagcccctcg tgctgcctga tcccaataag gagcacctag cccaacgaat
    17161 gcaggaacaa cataaacctc atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg
    17221 ctagcttttt gcaacatttg aagtccatct ctcagccatt gaatcgaaga accagccaca
    17281 aaaatacttc cttctaaagc atagtaaacc tttccattta tgccgtaacc aattgtcgtc
    17341 aataaattat ttttggataa ttgcggtttt tcaccggtat tcatgacaat aaatgaacct
    17401 gtaccataag tattcttgac catgcctggt tcgaatgcca tttgtccgaa taatgcggct
    17461 tgctggtctc ctgccatccc tgaaattggt gcttcgcttc cataaaaatg ataaccttgc
    17521 gtataaccat aaacttctga gttagaagtg acttttggta acatcactcg aggaatattt
    17581 agtaaatcta aaatctcctg atcccaatct agctgatgga tattgaacaa catcgtccga
    17641 ctggcattcg agtaatcagt aacatgtgct tgtccatcgg tcagtttcca tactaaccaa
    17701 gaatcgatcg ttccaaacat caattcgcct ttttcagctc gttcttgcgc gccttctaca
    17761 tgatcaagaa tccagcgtat cttcgttgct gagaaatagg catcgatcac taaccctgtt
    17821 ttttcgtgga aaaattcttg atagccttca ttttttaagt tatctgcaat tccagctgac
    17881 tgtctggatt gccaaacaac tgcatgatag attggacgtc ctgtatcttt ttcccatatt
    17941 actgttgttt cacgctggtt cgttatgcca atcccagcaa tctgatttgg tttgatccct
    18001 gattcgatga acgctccggc aataactgac tgtacagaat tccagatttc atttgcatca
    18061 tgttccaccc aactttcttg cgggaaatat tgcgtgaact ctttttgaga actgccgatg
    18121 tgtcttcctt tcttgtcaaa gataattgct cgtgagctag ttgtaccttg gtcaatcgcc
    18181 atgatatacg ttgattctgt cattgtgatt ccctccatta tatgcaaacg ctttctttaa
    18241 ctactcttat catactactg ttttaaaaaa agaaactgtc aaaattttat tctttttttc
    18301 tttttttgac agttttttat tatgaagcta ttttttaatc aattcttttc ttttttcagc
    18361 gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg acgcaaattc tgacagataa
    18421 tattcacact gttttttgtt tctctggcaa aatgtgatca ccaaatcata ttctttacct
    18481 tcttcataac ttccaatctc aataccgccg attcccctca gttctctgat cagatattgt
    18541 tgaaaagcaa tacgataaat cggcagactt tctaaatcga ttccaatcac tactggtttt
    18601 gcaatataaa aatcgatcat caaaagaaca ttggcatact ggatcatcag gtaactaagc
    18661 tcactatctt cagctctttt cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc
    18721 agtaactgtg ttaaggaatg cccgagcatt ttctgctgct gttccaaaag ccgatcacgc
    18781 tcatagactt cgatctttcc aacaaagaaa aaccattcat tgttgatctg ggaaatttgg
    18841 tagcctactg ccttttcttc tttgattttc aaacgacgag gacgataatg ccataacatc
    18901 gtttcccgaa tgtaagtatc taaaacagcg gttggtaact ttttgctccg tgtcaaatca
    18961 tactgataaa atatttcttc gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc
    19021 attgtttcgt atacattcag ttctgctgct gttcgactaa gatgcatcac gatgattgga
    19081 tcgattgctt gataaagccg gtccgactga tagagtaatt tcttttcttt caaagttgcg
    19141 tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc cgagccagca agcaatctgt
    19201 gcttctgctg atgccgtaaa cgttgtttct aatacacggt ccaatcctag aatgaacggt
    19261 cttttttctg gtgtattttg caaaaaaagc ggtcgctgat cttccgggat atattggaat
    19321 agttcataat aaaaatatcg tatctgtagc tcttctccgt ataattgacc gtttttgatt
    19381 tgtaatccaa attcctctaa gagctggttc aattctttga tctttcgaaa aacagaagac
    19441 tcactgatat taaacgctgt cgctaatcga acgatggata cttgctgatg ttctaataaa
    19501 tagacgagca tcttaaactt caagctttcc tgcaataaaa acaatagaat tttttccaaa
    19561 cttacatctt cgtccaacac aagcagcgcc tgatactcat ttcgctgtac ttctactttt
    19621 ttttccatca ttcggccaat caattcaata tcttccaaat attgatcaaa cgaggctttt
    19681 gacagttgga catgctctct caaaccattg atagaggctg ttcccccttg cagaacaagg
    19741 tttcgcaaaa tcaggatctg taactgctct tttctttcta ataatgcttc aatcttcata
    19801 ggcagtattc gccacctttg accacaccat atctggcaca cggtattctg gctggaaaaa
    19861 tagctcatgt tcttctaact gttcaaacgt ccatccttct tctactgcaa ttgtcagttg
    19921 gtcaagaagg tggaacatct cttttttgct ttggagctgt gctccaagaa tctttttttc
    19981 agaatcaaat agaaatttga gtttaacttt gctttgattt attgctgtaa gcttgtagtc
    20041 tacgtatcct gtttcaattt tttggggata aaaaatgctc tcttcttctg tcaaacctac
    20101 gcttcctaag aaatagccaa accactgatt gccgactgga cgataggttc cctgatcctt
    20161 tgtctgtgag ccactaattg tcttgctgac atttcgtgct gtacggacag cgttatttac
    20221 tagagaaaca tacatttttt catttgttgg acgaaaagtt acttggatcg catcgccaat
    20281 cgcataaacg tctttttgag acgtttgcaa gtagtcatct acccagatcg ttccatcatc
    20341 attcaatgtt aattgttctt cccacatctg attgtcggga cgagaatgat tagccagcaa
    20401 aacaagatca tagttctcct ttcttttttc cgtctctaca atagcggttt cattttcggt
    20461 caaagacaca acttgttctt ctaaaaaaag tgaaagccct tgatttttca tttcctttac
    20521 taacggttct gtcatttcag gatctagata acgaaaaaga attgactttc ggctttcgta
    20581 aagatggatt tgtttttttc ttgatgccaa cccttcagct atttcgattc ctgcttgtcc
    20641 tgcaccaata attgcaattt tctttgcatt catgatcttt tgctgaagag ccgttacgtt
    20701 ttgtatcttt ttaactgggt gaataagcgt tgattctgca gaaatatttc gaaacttttg
    20761 atttgaacca gtcgctagaa tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac
    20821 gacttttttt tctttcaatc ctattactgt cttttctgtg taaacatgga ttcgatagcg
    20881 gtcagtcagt tcttctttcg tgatccaaga aagttgtttt tcactggtaa cctttccttt
    20941 taatatcaaa ttgaacccgc tgggaatcca tcctaacttc gattgctttt caatcaaata
    21001 tatttcactt gttggcatca attggcgaag ataaatagcc gcatgaatcc ctgcaaacga
    21061 cccgccgaca attacaatgt tcatctgcat tcctctttct tataaggtgt tctactgcta
    21121 acttctcaat cagaaaacac agtattcata actcgctttc aaccttatga tacatagcga
    21181 tgcagcagaa tcaaccctct ccatcatacc gtgttctagg tctgcttctc aataatgtac
    21241 tagtggacct aattgaagag tatcatttct gaagagatgt ttcaaaaaat gtactcttat
    21301 aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa
    21361 aagacgagtt ttagcgtgtg taatgtaata gttagaatag acacatcatt tatttttaat
    21421 tctttaatgc ttcaacaact gcttcgccta attgatgagc agattgagga ttttgccctg
    21481 taagaagccg cccatcaatt tgaaccactt ctgaaaaagc tgctccagac tgatagtttg
    21541 ctcctttttc tttcaaagca tcttctgcta agaatggtac ttcattcgtt gtaccatttg
    21601 cttcctcttc ttcattactg aacccagtca ctgtacgtcc agctaaaata gattttccag
    21661 actcatctgt aactggcaac aatcctacaa caccgtgaca aacagctgag accagccctt
    21721 catttcgata gatttgtgaa gcgactttcc caagatctat tgcttctgga aaatcccaca
    21781 ttgttccgtg gccaccagca taataaaccg ctgcataatc atttgcatcc acatcatcag
    21841 gacgtaaggt atttcccaag gcccgatttc tgaaatcttc atcttcataa taggcccaat
    21901 cgatttcatc catattcaaa acactttgcg gttctaaagg tacatatccg cctttaggac
    21961 tgacaaaatc tgcatcgatc ccatttttta ctaatacatc ataaaaatga gtcagttcac
    22021 ttaaccacaa acctgtagga cgctcgatct tttcgtactt ttctacattt gttaatacga
    22081 tcaatacttt cattttccca tcctctttct ataaaaattg cgttattgat aacgtgcttt
    22141 caagaaatta tataatgggc ttggcaaata aagctcttta tatgcttttt caaaaggttt
    22201 cgttacaaat tcatcctcac ggttttccaa taatttctct gcccagtcag gatcgacgat
    22261 catttgctgt ccaattgctg caagttctgc atatttcaat acctgctcta catcttcgcg
    22321 attgcggatc ccaccgacac caactaaagg gatctttcct tgaatcgtgt tatggaccac
    22381 ttcaagaatc gatttatcgt tgtatttatc agaacgagct attcgatcat acatatttaa
    22441 tgaaacatgc agataatcta gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc
    22501 ttcaaaacgg atacctggtg tttcatattc ttctggtgag aaacgatacc ctacgatgaa
    22561 tggtttttta gcatattgtt caaccgctga gaaaaccgtt gttaataatt cttcaataaa
    22621 acgataacga ttttccaagc taccgcccca ttgatcctct cgtctattcg aatgcggaga
    22681 gaaaaattgt tggatcaaat acgtatttgc tccatgcaac tcgattccat caaatcctga
    22741 ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt gtttcaacga tttcttcttc
    22801 actcattgct cgtggtgttt ccgcatcagg acgttttgct gcaactgcac ttgcagatac
    22861 aggctgttct ccagataaag ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat
    22921 aatcgctttt gcgccttctc cttggattgc tttcgctagc tcacttaatc gaggaatcat
    22981 ttcatcatga gcgatgctga gttcacctgg ccagcctttt ccgatatctt ggacatttgc
    23041 agcaccagta ataacagcac caagaccttt agaacgttgt ttataataag tgatctcatc
    23101 ttcggtgatc actccattga aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg
    23161 gttcttcaat tgaactcctg aaggtaaagt aattgattga tcaaattgtg acattgtcat
    23221 tcctcccata attcgctctg attaacctat cgagaatcat ctatcattaa accactcgat
    23281 tagttgtaca tgcaactaat ttgcttaaaa aattttattt tttttgtaac ttatctgttg
    23341 catcattcat tgtctgggct aattctttac ttgcttcatt tcccaaaact gctttgtatg
    23401 actgatgaag ttcttcccag ctaccatcga cttccttagc aaattggcgt ccttgttctg
    23461 taagataaac taatgacaag cgaccttcgc taactgtttt gacgtatcct ttatattcta
    23521 atttttcaac aaaacgagca atcgttgatg gggcggaata gagcatttgt gcaatatctt
    23581 tttgtggtat tccatctttt tcttttaata tcaataaaat aaaaccataa gttggtgcca
    23641 gacctgtcct tctaaacgct tcatcagcta atttattcaa catgcgatct agctttttca
    23701 cgctaaagaa aagacattca tctaacgctt tttcaattgg cttttccatt tcatcccctc
    23761 cattggcgta catatcatag tatgattttt attttttgtc aaaaaacacg gaataatgtc
    23821 cgcctcctct gtctagattt gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt
    23881 tttcgacaaa actccttctg gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt
    23941 ttgagtatga agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    24001 aataccgaaa aactggcttt tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct
    24061 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttgtc gttttttgag
    24121 ctaatgccga tggtcagctg attgtacacc gtttattgag aggtcgtgag cctgccgttg
    24181 aactccgagt cacaaggagc aatttcaaaa aacagcttct catgttttat gactatggtg
    24241 acttgtgatc gaggagttgg caggagaata ctgtttattc gtaaagaagg ggctgtgaca
    24301 agacttttgt cacagtccct tatttccttt tattatagga tttcgccatt tgattggatg
    24361 acttgtctgt accaatcaaa tgattttttc tttaaccgtt tctttgtgcc attcccttcg
    24421 tcatcttgat caacatagat atatccataa cgttttgaca tttgtgaagt agaagcgctg
    24481 acgatatcaa tgcagcccca cgacgtataa ccaaataaat ctactccatc tttgatagct
    24541 tccttcatct gctcaatatg ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt
    24601 ccatcttctt ctactttgtc tatagctccg agtccgtttt cagctatgaa cagtggtttt
    24661 tgatacctat catataattg attcaaagca attctcaaac ccttaggatc aatctgccag
    24721 ccccattcac ttgatggaag atacggattt ttaattcctc cagttattaa gtttccttgg
    24781 accccttccc aattttctgg atgagcactg actgcactag acatgtagta gctaaaagaa
    24841 acaaaatcaa cagggtaggc tgctaagatt tcttcatcac ctacttctat ttttaagttg
    24901 acaccttttt cttcaaacat tcgttgcgta tacgctggat aatatcctct tgcttgaaca
    24961 tcactaaaga acaaagaagt tctttgctgc tcaattgttt tttgaatatc atccggatca
    25021 caagaatacg gaacaagaag ctgatagctg atcatacaac cgacttgtga acctttgata
    25081 atttcatgac aagcttttgt agctaaagca cttgctataa actgatggtg tgcaccttgg
    25141 tatttcgctt gttccaaatg gggatggttt tcttcaatta ctcctacgct aacaaaaggg
    25201 tgatgtttta cacagttgat ctcattgaaa gtcatccaat attttacttt atttttatag
    25261 cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa taagctttcg attaggccat
    25321 ccaccatatt ctgtaaccaa atataacggc atttcataat gggaaagaga aacgactggc
    25381 tcaatcccat attttagaca ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca
    25441 ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc atgcgataga catccgatag
    25501 catttaaaac ccatttcagc aaataaacgg atgtcttctt taaagtgatg atagaaatca
    25561 tttccgtgtc ttttaggata ataatacttg ctgttgggat cctgcgccgc ttttacttgc
    25621 tgatgcgtca tcatatgcca ttgatctgtc catttttctt taggtaaatc gctgtcgaat
    25681 gtatacacat cagacacaga taatcctttt tcatctacat cccatgcacc ttcacattga
    25741 ttggccgctg tcgctccgcc ccataaaaat ccttctggaa attttttcat atcgatcccc
    25801 ctaaacttgc tagttttctt gtttgattat ttttcttatg actagtaatg atatttttga
    25861 atctactact atttgtaaca ataaccattg tggtcggatc atatccctct ttgattaatt
    25921 cttgcaaatc tacttccatc aataaatcgc ctttacgtac tttttttcca tcagatactg
    25981 cactttgaaa gtatttgcca tttaaattca ctgtatcaat accgatatgg atcagtatct
    26041 ctgctccttc ttcactgact accccaattg catgtttcgt tggaaaaact gttgaaactt
    26101 ccccgtttac agaactgtag atttttccgt ccgttggaat gatcgcaaac ccttctccta
    26161 aagatccact agcaaatgtt ggatcgttta cttcagataa atcaattatt tcacctgctg
    26221 caggagtact taacactata tcctttgagg attcttcttc gatttcatcc ttgtagccga
    26281 aaagaaaagt tagcatacag gaactgatca tcgctaagaa ataactagca actagccaaa
    26341 caaatccttc accaatataa atcgggaaag acaaaatact tcttgtagca actgccaaag
    26401 caccggctcc tgcggcacca gctactccac caaatatagc tccacatata caagcaatgt
    26461 aaaatggtcg tttatattta agagtcacac cataaacagc aggttcagtg attccaaata
    26521 atgctgtgat aaatgcagat aaagataaac tttttacttt cttatttttc gttttcaaaa
    26581 ataccccaag tactgctcct gcttgcgcag ctacggcagt catacatgct ggacctagcg
    26641 tatcacgacc gtagaaagaa agattgttgg tcattactgg tacaagtccc caatgtaacc
    26701 caaaaatcac taacacttgc caaaatccgc cgataacagc accagataaa acagggctaa
    26761 agttataaac agctaaatat ccagctgcaa tcagctgact tgcataatct gcaattggac
    26821 ctacaactaa taaaataatt gggaccatca cgatgataca aatgaatggt gtcaataaat
    26881 ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc aatataagaa agaacccata
    26941 cagctaaaat tgctggaata accgtacttg tataccttgc tagaatcacg ggcatcccta
    27001 gaaatcgcaa ggttatgcta tcactatatg cactgacaat cgtaggataa acgagagctg
    27061 ctgcaataac catagcgatg aaacgatctg tattgaactt ttttgcagca gtatatgcca
    27121 aaaatatagg caagaaatag aaaaatgcat cagctgctgc attcaaaatg atataagttc
    27181 ctgattcagt agttaataaa ttcaaactcg tacataaggc gagtactcct ttgatcaaac
    27241 cacttcctgc caagacgcct aaagtcgggg caaaaacagc ggcgatcatg tctataaaag
    27301 agtttaaaat gccttttttc tcagttcctc tctttccact agatgcatca tcttctagtt
    27361 tcatttgttt ggataatacg tcatacactt catttactgc atttcctact acgacttgaa
    27421 acattcctcc agcttcgact acagtgatca caccttctag ttgctctacc ttctccttat
    27481 ctgccttctt attatctttt agggtaaagc gtaaccttgt tgcacaatgg taaacattgc
    27541 tgacattttc ttcgcctccg acaaatcgga caatatcttt cgctaattgt gaatagtcca
    27601 tttttacttc ccccttgtta attaatgccg tagcaatcct tcttacaaga taagtatagt
    27661 tcttagaaaa cggtttctcc attcaattag taccccttct aatagcgaac gaaactgtac
    27721 aatagtgtgt atggtttact tagccgagca aatcactcac tgcttgaaag aaaacaagta
    27781 tatcgtttct cttatagtca aaggagacaa aaaaaacacg cttgatcttc ctttgctgat
    27841 gtataagtgg tatctcaagc tgatactctg tgatttttgt ttctacttgg cttttgataa
    27901 gacagcacaa caaagtctcg gcatttaacg gaagcataac taggtcagaa ctaggagaga
    27961 ttagttgctg aatggaatct atctttttat ggatttttat ttgattttct tctttgtagt
    28021 aatgatcaag cagttttgtt ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa
    28081 tctgataaat ctcccaagat tcaaactcat ctagaacact aggtagataa atcaaatcat
    28141 cccactcaaa aataagctct tgctcagtag tcaaaattgc atcatattcc gttattttgt
    28201 ttgattgatt tttgaaaaga tacactggtt ctatccggca ttctttgatc cacatgttca
    28261 tactttgttg aatctgtttt tttagtgtat tcaaaatact aattggctgg ttactaacaa
    28321 tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat
    28381 aagcgctttc agctaaattg ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta
    28441 aataaatttc taaaggataa cgggagatga tcttttgtac aaaatgattc atggtattat
    28501 gtccggcatc catcctcgtt ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat
    28561 ggaacaataa cgaaacagga agatctaaaa aacgaataat cgtttgctca aatgctctta
    28621 gattcttctc tatttctttg tttttttcat cattaatcat caaataattt aactctaata
    28681 gtcgattttc aattgcttgt t
//
LOCUS       DAYYME010000024        26715 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000024 DAYYME010000000
VERSION     DAYYME010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.024"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..204
                     /locus_tag="ACHBFW_000853"
     CDS             <1..204
                     /locus_tag="ACHBFW_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HFM9317186.1"
                     /translation="EKNTKRIVGVQLCSQEDLSGLLSMFSLAIEEQITLDKLKMLDYL
                     FHPSFSQPYNFILKAVTGLNIET"
     gene            316..1767
                     /locus_tag="ACHBFW_000854"
     CDS             316..1767
                     /locus_tag="ACHBFW_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM9317187.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            2116..2748
                     /locus_tag="ACHBFW_000855"
     CDS             2116..2748
                     /locus_tag="ACHBFW_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFM9317188.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3336..4775
                     /locus_tag="ACHBFW_000856"
     CDS             3336..4775
                     /locus_tag="ACHBFW_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9317189.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            5070..5288
                     /locus_tag="ACHBFW_000857"
                     /pseudo
     CDS             5070..5288
                     /locus_tag="ACHBFW_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            5537..5752
                     /locus_tag="ACHBFW_000858"
     CDS             5537..5752
                     /locus_tag="ACHBFW_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317190.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5969..6225)
                     /locus_tag="ACHBFW_000859"
                     /pseudo
     CDS             complement(5969..6225)
                     /locus_tag="ACHBFW_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6412..7323)
                     /locus_tag="ACHBFW_000860"
     CDS             complement(6412..7323)
                     /locus_tag="ACHBFW_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9317191.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7342..8346)
                     /locus_tag="ACHBFW_000861"
     CDS             complement(7342..8346)
                     /locus_tag="ACHBFW_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM9317192.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8343..10466)
                     /locus_tag="ACHBFW_000862"
     CDS             complement(8343..10466)
                     /locus_tag="ACHBFW_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM9317193.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10471..12918)
                     /locus_tag="ACHBFW_000863"
     CDS             complement(10471..12918)
                     /locus_tag="ACHBFW_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9317194.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12905..13294)
                     /locus_tag="ACHBFW_000864"
     CDS             complement(12905..13294)
                     /locus_tag="ACHBFW_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9317195.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13354..13857)
                     /locus_tag="ACHBFW_000865"
     CDS             complement(13354..13857)
                     /locus_tag="ACHBFW_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM9317196.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13870..14094)
                     /locus_tag="ACHBFW_000866"
     CDS             complement(13870..14094)
                     /locus_tag="ACHBFW_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317197.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(14198..16108)
                     /gene="ltrA"
                     /locus_tag="ACHBFW_000867"
     CDS             complement(14198..16108)
                     /gene="ltrA"
                     /locus_tag="ACHBFW_000867"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM9317198.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16854..16991)
                     /locus_tag="ACHBFW_000868"
     CDS             complement(16854..16991)
                     /locus_tag="ACHBFW_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM9317199.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(16988..18172)
                     /gene="mobT"
                     /locus_tag="ACHBFW_000869"
     CDS             complement(16988..18172)
                     /gene="mobT"
                     /locus_tag="ACHBFW_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM9317200.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(18431..18721)
                     /locus_tag="ACHBFW_000870"
     CDS             complement(18431..18721)
                     /locus_tag="ACHBFW_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317201.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18711..19853)
                     /gene="dcm"
                     /locus_tag="ACHBFW_000871"
                     /pseudo
     CDS             complement(18711..19853)
                     /gene="dcm"
                     /locus_tag="ACHBFW_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(19929..20093)
                     /locus_tag="ACHBFW_000872"
     CDS             complement(19929..20093)
                     /locus_tag="ACHBFW_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317202.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(20086..20760)
                     /locus_tag="ACHBFW_000873"
     CDS             complement(20086..20760)
                     /locus_tag="ACHBFW_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM9317203.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(20838..22178)
                     /locus_tag="ACHBFW_000874"
     CDS             complement(20838..22178)
                     /locus_tag="ACHBFW_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM9317204.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(22261..22596)
                     /locus_tag="ACHBFW_000875"
     CDS             complement(22261..22596)
                     /locus_tag="ACHBFW_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFM9317205.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(22606..22980)
                     /locus_tag="ACHBFW_000876"
     CDS             complement(22606..22980)
                     /locus_tag="ACHBFW_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM9317206.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(22993..23307)
                     /locus_tag="ACHBFW_000877"
     CDS             complement(22993..23307)
                     /locus_tag="ACHBFW_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM9317207.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(23420..26647)
                     /gene="fss3"
                     /locus_tag="ACHBFW_000878"
     CDS             complement(23420..26647)
                     /gene="fss3"
                     /locus_tag="ACHBFW_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM9317208.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
BASE COUNT         7762 a         5556 c         4654 g         8743 t
ORIGIN      
        1 gaaaaaaata caaagagaat agtaggtgta caactttgta gtcaagaaga tttatctgga
       61 ctattatcaa tgttttctct tgctatagaa gaacaaatta cattagacaa attgaaaatg
      121 ttagattatt tatttcatcc tagttttagc caaccctata attttatttt aaaggcagta
      181 acaggattaa acattgaaac ataatcgtta ctaaatgaaa tcaagaaata tactgtatta
      241 ctcaattcat tgaaaatatt tatcataatt tttaatagaa aacactatta aagagaaaaa
      301 agagaggtaa atcaaatgaa aaaatcttta aaacttgcat cagcagttat attaatgtcc
      361 caactcctta tagcaacacc tttccatgtg ttggcaagcg aaaattcaac atacaaaatt
      421 gcaaaagaaa gaaccgagac gactgaaatg agtaattccg atgtaacagc agagagtagt
      481 gaacttttga caacttcatc aagtgaaagt agtacgactt caaatgccga gtcttcaaca
      541 acttcatcaa gtgaaagtag tacgacttca aatgccgagt cttcaacaac ttcatcaagt
      601 gaaagtagta cgacttcaaa tgccgagtct tcaacaactt cgtcaagtga aagtagtacg
      661 atttcaagta ccgaatcttc aacaacttca agtagtacaa gtgaaataac ttcttcgaaa
      721 gtgacaaaac caaaccatat agaatcttca aaaaaggaag cgccaaaaac ggaaggacaa
      781 acaaaaactt tttcgacacc acagcaaata gaatcaacag aatctttcca atcacaacca
      841 atactatctg gtaatgaaga aattcatttt gacaaaaatg aatctgtaga aaattttatt
      901 atgaaaattg gtgagtctgc gagaaaaatt ggtcagaaat atgatttata tgcttctgtt
      961 atgattgcac aagcgatctt agaatctgcc agtggtcaaa gccagttagc tcaggcaccg
     1021 aattacaatt tatttgggat aaaaggaact tataatggaa actttgtcat tatggtaaca
     1081 aacgaagatt tgggtaatgg aacgctttat acgacccaat caaaatttag agtgtatgaa
     1141 aactatgagg aaagctttga agattatgcc aaattattaa ctaagggaat tagtggaaat
     1201 aaagattttt atgcaggagc attaaaagct aatagtaaaa catatcgaga agcaactaaa
     1261 ttccttacag gacggtatgc aacagataca caatactatt tgaagctaaa tgaattaatt
     1321 aaaacctacg atttaaccaa ctatgataaa gaagttaaag gtccacaaat gagtaagact
     1381 ggataccaag tgcctattca taattattca ataaccagtt tgtttggaaa tcgtggaaat
     1441 gaatttcacc gaggacttga tatggcagct acacagggtg agccgattca tgcagttaaa
     1501 gctggtaagg ttgttaaagc agagtttcac tactcttggg gaaattacgt ggtgatacaa
     1561 cacgaagatg gatcaaccgc gttatatgct caccagcaac aatacctagt aaaaactgga
     1621 gataatgttt tgcaaggaca aataattggc tatgtaggaa gtactgggaa tagtactggc
     1681 ccacaccttc atttggaaat ttgcaaagat agtagtcttt ctcagagcat gttagttgat
     1741 ccgaaaacct tattattcgg agactaaaaa aggtaatcaa tctgacaaag taacaagaaa
     1801 atggttggat tcttagacct ttgatcagga tattgagact ggaattgata tctcgattac
     1861 gatgggtttg acaaataaaa caagtccatt atcgaatatc gagtgtatta ttgccgtctt
     1921 tatgctcata ttttgagcag gtttcactag atgaaaacaa tttatctatc ttgaggattt
     1981 cgtgtacgta tcgttcagtc ttgtattgta aattagtcat aaaatcagac taagaaaaat
     2041 tggaaatatt tttttctgat ttattaaaag ttaattgaaa ttatatggta cttctttctt
     2101 gggagggtag gtgcagtgaa ggaaagatat ggtcacctta ctgttgttga ggagattact
     2161 cataatggat ataaaaaata tcgttgccta tgtgactgtg ggaatgaaac gattgtatat
     2221 cgatcaaact tactatcagg tagaaccaaa agttgtggat gtggaaatta taaaaatagg
     2281 cataaatata aaaatttgac aaataaaact tttggtaagt tagttgcaat caagccaact
     2341 aaactgcgaa gtgaaggaac tgttgtttgg gaatgtcaat gtctgtgtgg gagaaaaaag
     2401 ttagtatcgg caaagcgact tctaaaaggt gatgtaaaaa gctgtggatg tttgaaatca
     2461 aaaaagcatc acattgaaaa taaaaaattc ggattactta ctgcaaagta tcctttggac
     2521 aataactgtt catggaaaac aaaatgggtt tgtcattgtg agtgtggcag tatgtgtgag
     2581 ataagttata gtaacttatt ttttggacat actcaaagtt gtggatgttt aaaaaggcaa
     2641 gaaacaagga catgtgttga agggacagta atagagtgtc taacgtcaaa attaccgaaa
     2701 aataatacaa gtggtgttaa gggagtttat cgttctaaag agaaatagat tgcgtatata
     2761 actttaaaaa aaaagaggta ctatttagga tcgtatgatt ctattaagag tgcagctcaa
     2821 gctagaaaag atgctgaaaa taaattgttt caacctttga ttgacaaata tcgaggtaat
     2881 cattaattat tgtggataat aattaacagt gatatataaa catctataat atattattca
     2941 tactattgat atttagtata atcctatgga attgaatatc atagcaatgt atttgaaaag
     3001 caaattggaa aacaaatagg tgcaacacca taaatatgaa aaaattggga aaaagtagaa
     3061 tggaaatttg cttacgtctc acgagatagc tcacactatt aatctgaaga acaatgaagt
     3121 cgacttttag aaattgctag aagaaagtaa agcgaattat cactagttac tatagagtaa
     3181 gttcaaataa agcatctcca acttacatgg ttacatgagg atatcaaatc gaaattactc
     3241 aagcttccta tggctagagt cgcgagaagt tgttgggcag tagacatagg ttatattgtt
     3301 cttagctaca cgatcattag aaaggaaatc ctattatgta tagttatcaa gaagatgcaa
     3361 aagtccaaaa aaatcgctgg tggattttga ttgctatatc tatgtttacc ttcatgtcca
     3421 cattggatgg cagtattgtc aatattgcac tacctaccat ctcagatgat atgggcatac
     3481 caatgaatca agcggaatgg gtagtgtccg tttatctgat gattgtctgt tcttgtttgt
     3541 tgctgtttgg taaaattggc gatagccgtg ggaaaatcaa agtatttcgg attggtacgc
     3601 tgatctttgt cttgggatcg ctgttgtgtg ggttcaatca ttcgctaggt attttattgt
     3661 tcgcacgggt ggtacaagcc attggtgcta gtatgactat ggctacaaat gccggaatca
     3721 tcacggaagt ctttcccata catgaacgtg gtcgggcatt gggagcaata ggtgcgtttg
     3781 tttcattagg ctcgattgct ggaccaggtc ttggcggctt gattttgtcc caattttctt
     3841 gggcgtacat tttttggatc aatgtaccgg tgggaatcgt aacgatgttg attgctgaaa
     3901 aattcttacc aaaagatatc accatgagcg gccatacaat cgatggaggg ggctttgctg
     3961 ccattgcggc tacgattatc agcttttttg gagcaatctt tttggggcaa gaaagtggct
     4021 ttttccatcc agtccccctt gtactgttcg taatttcagt gattgccttt gtcagctttt
     4081 taaaagttga aaagaaagca gcctctccat tgatttcttt cggtattttt aagaacaaga
     4141 tttttaccat gagcttgatt acagccttgt tgattttttc ttccaacttc tttgtcaatg
     4201 tagtgattcc tttttatctg caaaatgctc gcgggttgag tcctagctat gctgggctgt
     4261 tgatgatggt ctttcctttg atgatggtcg tcggggcgcc tttaagcggc tacctgaccg
     4321 accgggttgg cccggagatt ttggtattag taggcttggg cttattgtcg gtaacccagg
     4381 tcatgtatat gtttttgtca gtggactctc ctttgtggct atacattgtc gctactgcca
     4441 tcatgggtgc cggcaatgcc ttgttccagt cccccaataa tacgatggtt atgagctcgg
     4501 tcactatcga aaacttggga ctggcaggct cgatgaattc ctttgcccga aatttgggga
     4561 tggtcagcgg gatttcgctt tctacgacgg tcttatatca agcgatgtct gctaagatgg
     4621 gccagcaagt aactaactat atcgcaaatc ggccggatgt ctttttatat ggaatgaaga
     4681 ttaccttttt aggcagcttt gttttgtgtt ttgttgcctt tttcctgact atctggcgta
     4741 tcaaaaaaca aggaggtatt cagaaaaatg tctgaaaaaa ccactgttga agaatttttg
     4801 gaggcctgcg tatgggtgag tgggatttcc atggatgatg gaagatgaag tgaacttcta
     4861 aaagccgaaa aactcaagta ttatcttcaa aatactagtg ctcaatgaga tgcttgattt
     4921 gatcacaaga agactgcgaa agccgctggc aaccgcaagc taggtatccc accgatggac
     4981 tacgttttag aggctactgg ttatgaatac ggagccaacg ctctcatctg cataagaata
     5041 catcacccag attatgtaaa gattttcgat acttctgtca aaaaatcatt aagatagata
     5101 aatgatttcc gtctagtcaa attggttcag aatgtagaca cacagatggt aagaagtcac
     5161 tcacaattcg agaatggact tgtcctattt gccgtataca tcataatcga aacgtcaacg
     5221 atagtatcaa tattttgacc aagggctatg aatacaagca ttgccttaga aaacatcaag
     5281 aaccttagga acaacgggga tagcttggtt aataagagat accgctgttt aggcttgcaa
     5341 gtatactctt ttatcaagaa ttccgtggct ttagtcatga gaggcttaat actatgaaaa
     5401 ttttctttag catttttgat ttaggttcct tttctaatta taataagatg accactacag
     5461 taacctactg aaatatataa acagccactg tagtaccatt ttcaaagtag tctatgagag
     5521 gagaatcttc attcttatga acgctggaat tgcggatatg aatttaataa aaaaaaccct
     5581 taatgatttt acttccaatt caatatcaaa aggtactggt atcaatttaa gcacaataaa
     5641 aaagctgaaa agtggtgaaa gatcagtgga aaagctgaat cttttagatg ctataaaaat
     5701 aacggaattt gctatgaaaa atggaaaagc tgagattgaa atatggagat aattatcgtc
     5761 tagtgaatta cttgccactt agctgaattt aacgatcctt ggtgcttgaa gggggagatt
     5821 cttgggaata gagcatactt gcaagcctat gtctttacta acaacgatat ctcttattaa
     5881 ccaagctatc cccgtagttc taaggctttt acttttatct aagcaataat cgtattcaaa
     5941 ggaaattttt gacccttaga aagtctcatt ttatcagacg tgagcttagg cattgctgaa
     6001 ttttgttaat tcggttttag gttgtgcttt gagcataata tgaaagtatt ctttatcacg
     6061 atttcattca tctaaggtaa tgtgatgtga ttctgaaatt ctttcaaagg cagtttttgc
     6121 ataagctgac attacatcat caatgacatg tttacaatgt ttcactacta aaataagggg
     6181 gtaaccaaga agaaaaactg aatgattatt tgtatctaat tccatggtat atcaaccatt
     6241 tcctatatag actgattatg acatggatta aaataaaaag gcactatgtc ttaattgtcg
     6301 gttttcgagt taccgaatgg cattcatcac ccacctatag aggatgggcg acttctgcct
     6361 gaaataagtt acactactta taacttctaa taaaattaga ttgagtcatt attatttaac
     6421 atcaacgatt ttccagtttc catttttttg taatactaat ttgtactgaa agtagctagt
     6481 ggtttttgat acattatcaa ggtattttat ctttgtactc actagaaaat tttttccgtt
     6541 ttgtagataa actggattct ctaactccga aaactttaag tcaatattga ttggtcgcat
     6601 cgcaccattt tcaacataat actccaattc tttttcagaa gctgatggat aaagtttaaa
     6661 aaatgtttct aaaaaatcgg tgatttcttc cgttgttttg gcatcaattt ctgaatcgtt
     6721 ttctacttgt tttgcttcat attttgcctt gtctggcttt ccaatcattg ttggcaaact
     6781 agttactaaa tgattcctgt tttcatcttc aaagattgtc actcgatatg cagaagaaat
     6841 tgattgcgtc ttttttccct ctgtaatatg ttgctccact aaaaatgaaa cgacaaattc
     6901 ttccgagtgt ttttcaacat ctagaatttt tacagattga acttcggaac tcgtgggaat
     6961 atctgctcga accatatctt ggattaaagc gagaccgttt tctgtaaggt actgttcgag
     7021 attactcatt ctcttttcaa ttgcttcttt gttatttttc catgaaaaat attctttcgc
     7081 aaaatcctct gtaaaacttt caacgccaga agtattcact accttctctt ttataaccac
     7141 tttttcatga gttgtatgtt gatcaatggc ggtgaaattt ttataaacgc caaaagcaag
     7201 actacaagac aataaaagcc aaagggcaag caccattttt cgatgcttac ccacggatac
     7261 aactcgttct tttttctttt ttgggctctt ttccttttga ttccgttcaa tttttatctt
     7321 cattcttttt cctcttttct cttatttgta tcggccagct ccgataagat gctgccgcca
     7381 atagttttcc gttagattcg tccaccccac tggatctcct gcatgaaaca tgcgattctc
     7441 ccctgcgtaa atgcccacat gggtcacata agtaccggca tcataggttg aatgaaagaa
     7501 aactaaatca cctggtttgg catcttttag atcaatatga gtcatggcat catattgatc
     7561 ctgagccacc cgaggaagtt tcaggtcaac tttggcataa caccattggg tcaagccact
     7621 acaatcaaac cccgtttctg gacttgagcc accaaacaca taggccgttc cttcatactt
     7681 aaatgcctca tcaaaaattt cttggactat tttgtcatca aactgagttg tagttaggta
     7741 ttgcttcact aataaacagt aaaacatatt gccataatta taccgccacc caccattttc
     7801 cttaatcgcg attgggttgg ggtaatcgac tttttgacca cctgattttt cctttgaaaa
     7861 agattcagct aaagcaaaac tatgcttctt cccgtttttc ccgacatgat ctagataccc
     7921 gccaccatag ttataggcct gaataatact atcttgatca caccctagtt ctttggaacg
     7981 cttaaccaaa gacgcaaaat agagacagcc ttgtttgatg gatgcctcgg tacttaatga
     8041 attaggtggt aatccggctg actcagaact ttgcataacg tcttccattg tgccaccaga
     8101 ttccacttga ataatcgcca aaagaattgg aatatactct gttatatccg attctttggc
     8161 atacttttcc accattgcac ggtgtttcaa tacttcttct gagacactag ttccgccagg
     8221 agaagtgcca gaactaccat ttccctcact gtcttcacca aacaacaaac ccacacacat
     8281 caaaataccc ataaaagaga tggtcagaaa agttaatgaa ataacaccca ctttcttcaa
     8341 gttcattttt tctcacctga ccttgatttt ggacgatggt tcattctttt ttgtactttt
     8401 tgattggtct gctttggcag agtggacctc tgtttcactt gttggaaaga atttttctta
     8461 cttgctgatt ggcttttccc ttttgaaatg atttcttgac tagttagttt tcgtttgatt
     8521 tcgggattca gatttgctct cacaatccgc tttggtgctt cttttttcag catcatatct
     8581 ttctgaggtt gagctgcttt atctattgga atattcgccc ttctttttcc ctgacgattt
     8641 gttgcttcac tcagtatctt gcgtttttcg tccattttcg catttcttcg ttgtcgttgt
     8701 ttttctcgca aatccactcg atccgctttt tcttggaaca gtccgcgctt gaattcttct
     8761 ggtgcttttt tcgacttttc gattccttta tggagattgt atttcaaatt cgttggcgta
     8821 ttccttacct gatcctttac ttgtttagtc ttatctttca ccaaagcttg ggtatccaaa
     8881 actttgccga tttttctccc agccaaattc attcgggatt gtttcttatt atttgaaact
     8941 ggattctcct tagttaagtc agatagtatt tcgtgatcgt tcggtaatcg tgaattcttt
     9001 cgtagagaag aaccggatgg cttaaatttg gattttgatt gttcctttgc caacttattt
     9061 ccgactgctg caccaccacc aaaagccaaa gctcgtccca catttcgatt gagttgccgc
     9121 aattttcgat tcattaacat ttgcggtttt cgcatgacgc gtcttcccat tgactggcta
     9181 tcattactct ggaggttgaa catactcata atgtcgccca atttgaaata gataccggca
     9241 aaggtcacaa tttgcagaaa agcaaccatg aaaaatggat aggttgccga aatattgaaa
     9301 aacatcgtgg agatgctaaa tgcagtcgta atcaccaaag taacacccgc tcgcatcata
     9361 atcgtattga ataaacgaat aatcgccttc tttcctaaac tttcataagt cggcaacatg
     9421 gacaataaga aactaattgg cagaaacatc gcaaagataa tgaagagaat ttgcgagaac
     9481 aacataatcc ctgttagaag aaaaacaaag aaggaaatgc caatattgaa caataccaaa
     9541 aagaccacgg tccctaaacg gttcatggtc ttggtaatac tgagattcgc attgtcattg
     9601 tcttcaatct ctgccttgac cgcttcttcc cgatctttgc ctttgttttc atctggactc
     9661 acagatagaa tcttgttgac ccggtcttca ccaatttctt ctatgttgga atcatcaaat
     9721 tgcagtagta accaaggttg ttggacttga atcgaaaaca aactatcccg aatcaaatcc
     9781 acactatctt ttccttggct ttctgaattg ggtacaacga tctcggttcc aagggtaagg
     9841 gccgcttcac taacatctga gctaaagtca ttgatttttg tgatataggt aggcgcataa
     9901 gcaatgaacg aacccgatag tagaaaaatc atcacaaaat taatcacggc tcgaactgct
     9961 ttcgtggttt ctcgttttaa taatccggta tacgccacat acacccccag agctaaaatc
    10021 atcaataata gaaacccaaa ataaaatccg gaggtctgga gtccattttc agtaatacca
    10081 gccaatgttt ggatattctt tccaatactt tcagccgtat ccgaaatgaa atctaatttg
    10141 taagcctctt gaaccacata acccgtggcg ttcgacaaat acaaactcac cgtccaaatg
    10201 aaattagtaa tggcataaag accatacatc acgcttttcc ctaagccatc accccaattc
    10261 caaggcaacc aatcccaaga gctatccaca aagaaatcta gttgatagtt gttcagtgaa
    10321 tatttggaat atagatttcc tgcttcaact gtatcatcaa ctagccctgt cgcctcggct
    10381 accgtaccaa ctaaactcaa tagaactatc agagtgagta tgctaataaa aacgattccc
    10441 acaatccgaa aaagtttctt tttcatcccc tcactccatt ctctcttgaa caggtggacg
    10501 ggtatcaaag gcatggaaga gttcttcaaa gactggatgt acctgaacca cgcctacccg
    10561 accatacaaa tcctgcatca agcattggcc attttccaaa tctcgtaggc gtttttgatt
    10621 gctttcatct tctcgatcca agccaaagaa ttccaaggtt ttcttgattt catgaatatc
    10681 ggtactgcga aacgcaaact tcaacccgat attattcttc aagcgttcat ccagtaaatc
    10741 atcactattt tgcgtgacaa agtaaacccc ggcattcata gcccgcccag cacgaacgag
    10801 cttattcgat aatgttttcc cttgcgctac ttgaagaaaa ctccacgcct catctaagtc
    10861 tacaattttg aagatttctc gattactatg gataaagtcc aaggcaaaag tggaaatgac
    10921 gattagcatg gaaacactaa gcaattccat ggtggtatat tcttcaaaag tggtttctgc
    10981 atctggtaaa accaaatccg ctacttgaat gatatttaac tgcctatcta aactaatcga
    11041 ctgggtaatc gaaccatctg agaaaagcaa atgggcaaaa tcataatcgg taaaggactc
    11101 gatgtgatcc gcgatatttt ccgcaactgg tgagccttct tttcgaagct cattaattac
    11161 ttgtaataat ccccgttgat tgctttgggt aaccgtccga atggccttac gcaacactgg
    11221 aaacttctcc ccatctcgac tagaaattcc tgttaaaaag gtcagaatat caattgctag
    11281 actttcagaa tccttctttt tctccataat cacataagga tcaagtaatc cttgattttt
    11341 tggcttacta gttaagttga gaatattgat ttcatccgca atttctggca aggtttcttt
    11401 ccagccaccc cgttcccctt ttggatcgac aatcacagct tgtccaccaa aaaggacggc
    11461 gtaataaact aagagattgt tactaaagga ctttccgcca ccgagtgaac cgaggaatgc
    11521 cgctgctagc gcattggtaa ctgaatcctt gaccccttgt gaggccaatg ccggtttcag
    11581 gtaaacattg cgccccgtat ctacgttata accaaagtaa atgccttcca actcccctaa
    11641 catctgagtg gcgccaaaac caagtccggc gagaaaatca gacgtaacat actggatata
    11701 atcattcatg tatcgcttac tagaaggtaa gaattcttca tgtagcccca tcatatcgcc
    11761 aaaaggccgg actagtttta cattggtatc atcataaaaa tctagcactt catcacaccg
    11821 gcgctttagt tcatctactg attcggcact tactcgaacc acatagctca gtttgtacat
    11881 ggattctttg gactgatcca aggtagtttc caattcatct acggaatcca atgcatctaa
    11941 gacattggaa tttgtttcat tgtcagattg ataggcatgg ttgtccaagt cttttaattc
    12001 ctttttctta ttgcgaacgg tagcaagtgc ctttttgttc gtcacaatct ccacattcat
    12061 actagtgtca attgggaatg taaattgttg ttgctggtaa tagaaaagtt ctgaggaggg
    12121 aaactccatt tcccccacaa ttgtattaat tgttaagtac gctacatatg attcatgatt
    12181 ctcatgttcc atgcataagt accggggctt ttcttcaatc aaacaacgac tgggacgtaa
    12241 taagtcgtaa cgtttcacca aagtttccga cttcagcttt ttctttggta gctgaaattc
    12301 atattcttca aatggtgtac ccttttcgcc atagatatgt tcaatcaaat acatcagatc
    12361 actcggtgtg actcgtctga ctttaaatct tcgagccaat ttactttcca ttagtttttc
    12421 aagcttggta tatcggcgaa tttcttcatt ggataaggaa acaaaatcgc ccatcaaatg
    12481 atgattcact ccatagacaa attcttgaaa gccggagaag aaagatttct tgagattctt
    12541 tagattgacc tcttcatctg tcgcaatcag tttgaagcca ataaaaaagc gataatcaat
    12601 ctgactatcg ccaatcatgg atactaatgc ctctgtctgt aaatcaattc gttgtttcgc
    12661 tacttctgtt aatcgcccgg taatttcctt ttttgatcgt tcttgcgtgg cacgaatact
    12721 actttctgtg gcaatttgta aggcatgaat cttgccttca cgattttgcg caatcaattg
    12781 acgaaagtta tcgtggactt gatatttctg ttcaggtgac aaaaaggagt aattgtaagg
    12841 tattaactca tagtaggcaa aacattctcc ctcttgatta aagacaagat tattttcaat
    12901 atacttaatc gggtacatag ttcacactcc ttaccagagt aatgggttcg tcccatcgtt
    12961 tcttctgttc tttgactttc tttcctgcga aagtgacctt aaatcgtaag tgatagctaa
    13021 taaaagatcg taaaaatccc aatggtttct taccgtcaaa cgttttttgc gacatgaacc
    13081 aagtaactgc caccggtata cctaagtatt ttaaaaaggc cccctctatc atagatagtg
    13141 gaggcacatg ggcaaatacg atgacaaaca gcaaggataa aacaaaccaa gccatctggt
    13201 taaaagtcac aggaaaaggc agttgaaagt cattaattgc ataaatgacc ttttcaactg
    13261 cccaaatact ggtgtagctt tttattttct tcatcaaatc attcctttaa aaagaaggat
    13321 agtttagaaa tcaactaccc ttcaataatt ttgttaatat ggatactcaa aaacacctcg
    13381 actcgttacc aaataattgc ctccaatctc caagtctcta ccacacgcat caaaatcaat
    13441 atacgtcttc aagtgttctg gtacttctcc aaaatcacca gcttctacaa tcaagaaata
    13501 tgctacatca ctcatatcat cgcaatctgg ataatagata atatcatcca catgctctac
    13561 catctcctca aaagaattga agaaagcatg ttgaatctca gaagcaactt caccaactgg
    13621 cgttccttca agttcctccg ctaagccaca caaccgatta atttcctcaa ttggcgtata
    13681 ctcatcaatc tcaaagggaa gttcataatc atgaattgca tattcctcgt attcgtcatt
    13741 taacccaatt cgttctttca cttcatcaaa atcgacaggt ggggtaaacc acgcccccac
    13801 caattcacct tcgttatatt tcccgaggtt cgcaatataa acacgcatct gttccatcgt
    13861 gcttccccct tacgcaccga caattcggtt aaacaaactg agtaagacat ccttcacccc
    13921 agaagcatta aagaccaatc cgaccgcaat caaagcaatc accaaaaagc caatcagttt
    13981 ggaaaactcc cgcttaaatc ctaaaaacaa accaatgacc acaattgcca gtaagactaa
    14041 ggattgggca ttggttaaaa accattgata tatggatagg taagttcctg atattataaa
    14101 tatctcaaac ttttcccccc attcacacgg agcgtgcgac tttcaccgca ctccgcgttc
    14161 catcgtgttt atattgaaaa gtatttatgt agtagaccta tatcaagttt aattcttgat
    14221 tgccagctaa tactctatat ttatttagtc ttatgagctg gactttattt agcttcaata
    14281 gagctatata tttttgtatt gtttcttctg ttttagcgtg tattagacgg tgtaccgcct
    14341 cttttaccaa aataagattt ttataatcgt catttccacc agctccagtt ggtacaatat
    14401 gatgacaatg tatttcatta gcttgttgaa attcttccag agttatagca cattttccat
    14461 gttgagcaga aaatagcgaa attcgattgt ctgcgtattc tattgtgcga ttttccaaag
    14521 ataatttcat taactggtac atcagtaatt tatcaacact tagattggtg tggataaatt
    14581 ttcttccctc tagagtgtag caattcgcta aatacacctt tgccattggt tttctgtgtt
    14641 ggatatatcc aacagggaaa ataggttcgc cctcgccagc aacataccga agcattttag
    14701 atttaccata tcttgttgcc tcctttgtcg taagttgtct gcctgttttt ctcaatctgc
    14761 cccttttgtt tgtacctaat ctgttagtga agatagccat tacagctcta ttcagtttca
    14821 tacaatccag atttacattc gttgcaatct gataataatt ttgcatacct aataccattg
    14881 aattataaag tcttgtttct tctaattccg ttttcccttt tgagggagaa gcaattttct
    14941 ttgcctgtga taccagcttg tctttttggt ttttgaattg tttatcactt atatgtgatt
    15001 taacaactaa cttctttctt ctcggtatca ttttaatctt gaaaccaaga aagtcggagt
    15061 atctttttct aacatttaca attcttgttt tttcttgtga tacttccaat tttaatcgtt
    15121 gttcaatcca ttgagtgatt gctattttgg ctttttcagc actttccttg tagcggcaga
    15181 aaactctaaa atcatctgca taacgaacca tatacatttc ctttaacttt gttttcttca
    15241 tttctttgta agcgttgctt ttacatgggc taccgctttt attgatatga accacatagt
    15301 tttttgtaac aggattttcc tgccattggc tttctaccca atggtctaac tcattaagaa
    15361 caatgtttgc tagtagtggg gatattattc ccccttgcgg agttcctttc tctggatatg
    15421 ttattgtacc gttagtcatt ttgattggag cttttaatat cctttttaaa atgaatatca
    15481 agtgcttgtc ccgtattccc atagcccata tctgttttat cagcttggaa tggttcacgt
    15541 tgtcaaagaa acccttaata tcaaattcaa tcacataatg tagctggctg atttgtaggc
    15601 ggttataggt tgcttttatt gcattttcaa ctgacctatt aggtctaaaa ccatagctgt
    15661 tttcactaaa tttagcttcg cagattggtt ctaatacttg ttttatacat tgttgtataa
    15721 gcctgtccca catacaagga atacctagag gtctggtttt accatttggt tttggtattt
    15781 cttttcgcct cactggctta ggtcgatacc catgtggact gcctgcaaca atgtatctta
    15841 cttttttgac aactgtttct gctggcaatt tgccaatatc tttaattatt gtaccgtctg
    15901 ttcctgctgt tttactgcct gcatttgctt tgatatttcg atatgccagt aatatatttt
    15961 ctctcgatag gataatatcc ataagatgag tgaaaatatc tccattctta cttttggcat
    16021 ataaatcatc aaaggtgttt tgcatttcgt aatattcagc gtgtcttaaa tcttctacac
    16081 acaatgtttc atttttattt ttaggcataa ggcttcactc ctttacttga aattgagtga
    16141 gcttttttat cttactcgta atccttattg aaacaacata atacttttct aatatataca
    16201 cgacttgagg gcattcctcc attctcatta caagaatttc ttaggttcgc cctctgcttt
    16261 tcagcattag ttcaactgct tattgttctt cgtcagttta ttctcttgat gagaacccaa
    16321 tacctttcct cgttccgata atcttatcta tacatatata tccttaggtc actagctata
    16381 agcctgctgg cttgataccg cctgtaacgt tatatggcat ttcataacct atattcttac
    16441 tttgccacac cagctacgat ttctcgtacc tttgcatttc tacaaagttc aaatttagac
    16501 ccgtacattc gctgtttcgt cagttcctta tagaacattc taaccataga tattttatag
    16561 acccccgacc tgttggatac gctacccacc tctggatagt tttcgataga agatatatgt
    16621 tcatcaacta ttacggtatc tttcagccgt ctttaccgag cttcaaacat tctgagtttc
    16681 ctcatacaat gcttgtcgga ggctcagggc aggcgtttca gagcgttact tcatcattcc
    16741 acctgtcaga ttatcagttc tcctaataac caaacttttt cgttctttta ttagtgcctt
    16801 agcttttcac taaggaacga gtcgcacata aattttgtcc gaaattcatc ttgtcacttt
    16861 cctttctttt ccattgctcg atccgctgct gcacttgcat tcatcaagca catcacagta
    16921 acaccaagca tactgccacc aaataccaaa aaaatatctt ttacaattcc catgacgatt
    16981 cctcttttca tataatcaaa ttttcaattg ccatactttg ttgctccaaa atctttttat
    17041 gtcggtctga taagcgtgca ttctgtacca tatcctggat ataggttgtc cgattcaatc
    17101 gatccaactc cttagccatt ttccacgtag gggcgacttg tcggcctagc caattcaagg
    17161 ttcgttcaat ggtatacggt tttggctcgg aagttaattg aatctctttc cgattccgtc
    17221 caataaaacg cccccattgt tcattcaagg gccagttggt tcgcctttta ccttctactt
    17281 tatccgcaaa tcgcaagtaa cgattgataa tggaaaacgt ggtgctctca atgctacgac
    17341 cttttaacaa atcttgaatc gcatgaaaag cacggtcatt tttcagtcga atctcaaaac
    17401 gatttttcgt ctcagtttgg tctaatggaa tccctaactt gatatattgt tcatagtctt
    17461 tttcatacaa acagaaataa acctcactct tgagactgcc gatatataag gtattgccca
    17521 tgccgtcctt ttcatcagcc tttaaaagtt ccccggaacg ataactttta aaggtacgaa
    17581 acaaggagat acattcttcc ttctgacatt ttttcgttag ctcaggtata tccaatagcc
    17641 ccactcgatc attgattgcc aagtccaagc gtttcatcac gccaccggcc tttagacaat
    17701 cctcgaaaaa gtcgtaccag ctacgctttt gagcgagtaa aaaagtttca aattgtcggc
    17761 aacctcgccc ctttaattct aaaagcacac ctttatcttc ttcatgagac aacatcacca
    17821 caatatcacc catgacatac tgttcttgat aggagtaaaa ggcataatct tcatggagca
    17881 tatactcaaa tttcaaatgg agaatttctt gaataatttt tcgtgcatcc gttgtgggaa
    17941 agcgaatcct tacatagtca aacagtaacg tcaaaggcaa atcgggatta aagctttcca
    18001 gttggtcaaa aattttcctc atggttttta tagatgcggt tacgccacca ttttctatct
    18061 gatttaagta cggccgacta agtccacaag ccgtagccaa tttattttgt gaaacgccgt
    18121 attcgttgcg tcggtctttt aatagccgat aatctaaatt ccgttgtgcc aagttctcac
    18181 ctccaaattc aaggtgttaa ctttttcaaa tgagttaaca cctatacaaa ttacttacta
    18241 tctttgatac aacaagcatt cttaatggtt gcttacttat tttttgtgtt tttgtacccc
    18301 cctgttagat acgggggtga gacatcatgg tcggctaacg ccgtccatga acaggcacag
    18361 caccacggag gatttctgct tcgcgaatcc tccgcggtca gcgcctaaca gtcagttgtg
    18421 ttggtttagg ttactcatct gtaataccat ttacttccaa ctctgctttc atttctaaca
    18481 agtattgacg aatatctggt tcttttgctt ggtccgcaat ccgctcaaat tctgcaatta
    18541 cttgatttat ttcaattcga gcatccgtct cctcattcat taaatatgga tcgccagcga
    18601 ctttgacacc attcttgcga atatcaaatt ttaaccgtcg caattgttct tctaattctg
    18661 ggtctgactg aatctttcca atcacccaat aaatttctcc aatcaactca tcataaacca
    18721 tgtacatcca actcttttaa tcttctggca atatcaaaaa tgaccggaac tgttacagag
    18781 tttccggctt gcttatacaa ctgactatct gaattgactt gagaatctcg atcaaaagcc
    18841 caatcgggaa agccctgaag tcgccaacac tctctaggtg ttagcttccg aatttgatac
    18901 tgatccgtga cgatcgcttg ttggtctcct gttagtaagg tatgaacact ctgttttccc
    18961 agacgacctc ggcgacttgt ggaattagga aaagccagat tcacactgtc ttctggcttc
    19021 acctcttgat attctgtttc attttttcga atcatgattt ttgtaccacc ttgtttcgtt
    19081 gacaaggtgg gggcggttcc agtaacttca taaactcgat taccttgttc aaactttccc
    19141 ggtaatagtc ctgctacaga gattccatcc cttgctggca gtgcgatttt gattccttca
    19201 tcacctgtta aggttggcgc tacgccatct gaaccataga cttgtccacc cattcctcga
    19261 ttggaaggat tgatatttac caaatttctg atgttttgat cagatgttga agacggtcca
    19321 atgataggaa atacttgttc ggtacatgcg tctcgagaat gtccgataag gaatatccgc
    19381 tcccggcttt ggggaatgta ggcctttgag ttaagcacct accactccac gtcatacccc
    19441 aatccatcca gcgttcggag gatcgtctcg aacgtagccc ctccttcgtg attgagcaat
    19501 cctcggacgt tctcaaggaa taaaagctta ggtcggagaa gagtggtgaa ccgagcgatt
    19561 tcaaagaata aagtacctcg agtatcggaa aatctttgcc gctttcccgc aattgaaaaa
    19621 gcttggcacg gaaatccgcc gcaaaggatg tccactggtc cgagggattg aataaactca
    19681 tctgatacac ttgtgatgtc atgcatttcc acctccttac ttgtatcatg aattgcttta
    19741 taactacgtc tagcaaattt atcaatttca caaaaaccga tacaatgatg gctcgcctgt
    19801 tccataccta aacgaaagcc accaataccg gcgaataaat ctaaaaatct catacattac
    19861 ctcattcatt gcaaagaaaa aagccaagaa cattttgctc ttggctcata cattattaat
    19921 ttgtcctact attttttatt taatccaact aatttccgtt gtttttcatt ggcctgatcg
    19981 gctacatatt taatcaccgt tttgaactct tttgcagtag cattttccga taaatccgca
    20041 atgctcttat tgtatttctt tacaattttc gttacttgtt ctttctcagt catgtaattg
    20101 aacctccaaa tctttcctac tataccctag tggtaaaaat aattctagat tatccgtagc
    20161 ataatgaact tcggaaatat tattcgtata atcttttatt atgatatgaa gcaatacatc
    20221 tggatgttta ctctttactt ttgcaatgtt ctctcttgat ttgaaaaaag cgttaataaa
    20281 tgcctttttc ggcacatgac gtccctctgc aatttcacgt tcttgtgtaa atttccaagc
    20341 gattttaggg tcttgataaa catagaaaat cgtcactcga aaatgttttt tcaacgcccg
    20401 cgagatattt ttttcagcac ttaatattgc aaaagttgca tccagaataa aagaatagcc
    20461 atgatctaag acatatctaa atgcctgctc taccaaccaa gaagatgctt tttgaaagag
    20521 actagagttt tttccattat aatcaggaaa ctgggtgcga aagtaatccg catcaataat
    20581 cacatggtta tcataattct cagctaatcc aatggcaacc tctgattttc cagccccagg
    20641 actccctgcc ataaaaactg cgtctcgtcc aacttcgggg gatttccctt tgataatata
    20701 agaaataaac ttatccttat ttgcttttgc atactctaaa taagaatcat tactttccat
    20761 attaattatc ccctttcttc ctactatata aaagtattat acgttacttg aagtataaag
    20821 tcagtattga agatcactca atcattcatc ttttctaatt tactaattga atctaagaaa
    20881 tcataccctt ttggtacaag tggcgtataa aactcactaa tcacactgcc acctgtatcc
    20941 acataacctc gacctttgat gtgcttcata aagaagtttt tttcgacctc cccaaacatc
    21001 atggaatatc cgagttcact catacgaccc aaagccacgc gaaagttgaa ttgatcgcgg
    21061 attccgtctc ctaaatattt cgcatccggc ctttggcaag ccaaaatcag aaagaaacca
    21121 gattgacgac ccaacatcac gatttgtttg agcttattca aaatcaccgc actttctttc
    21181 gtcgttaaca tctccatata agccacgtat tcatcaaaga ttaaaaagtt gggtggaagt
    21241 ccaagatagg cataattctc tcctgttttg tagttagaca tttctttcat tgccttgcta
    21301 cgagccatca tacgttcata gaaatcttcc acacaagcag aaatttcttc cttttgtgaa
    21361 taaacatgag gcatcaccgt acctaaatca gctaaatctg catttttggg atcgaggata
    21421 aacaattctg catctgactt caatagtgct tcgataatgg tgaggaggaa ataggtcttc
    21481 cctccacccg tgccaccagc gattaacata tggggtaaag aatcataagc ccatacttga
    21541 tttttcatca atcgcagagt cccattttct gccactactt cgtctatccc aattcgattg
    21601 gcgatcatat catacagtaa agtatactcc acataggagt ctttgagttc cttttctact
    21661 aactcacaat acagtccgct ttctaacttc ttttccaatt ttaaaagctg atcttggtat
    21721 ttcccaagtg aaatttgaac gcgaatagac agtaatccat ctttcattcg ataacagatt
    21781 ttggggaagt aactgatagt ctctctggtt cggctactat tcaaatcttt gaaaaagcct
    21841 tcactttgag tttgtttcac ttcataccaa tggttctcta gaatcatccg agctagcttt
    21901 tgtcgatgcc acaagcttcg ccaatgatca agaaaaaagt gaatataaag caatatcata
    21961 gctcctgaaa aagcgagact tccaagcaga catactaaat cttttataga ccaaacgatt
    22021 tcaatagtag aaagtgatag cttagaaata tcaaattgtc taaactcttg aaaataaaag
    22081 acgctcatcc aaccgatgaa cagagcgagt agccagccaa gaacaaagtg ataaaccaag
    22141 tgctgatctc ctgcgcgtat gcgatgtcct ttgtacattt tgatggtttc cacctccaat
    22201 cgaaaaagcc caataggatt tcattaggca atttcaaaaa gttatgctag tcatttactg
    22261 ctacgaggag gggagttgtt tcgtttcaac caattgattt aaaaagcctt caatagcatc
    22321 cggcatctcc acaccaattt cttccaaaat ggattctgcc tgccgacata agttttcatt
    22381 tacttcgaca actataaaga aagactcttc ttctcctaac cagtctagta catcagagtc
    22441 ttcttcactg tcgctatttt ctccaagtaa accttgatgt ataatcagaa acattttttc
    22501 atcagaggtt aatgctttaa cgagttgttc ctcagtcagc tcgccttttc catattgatc
    22561 gagacaatct aaaatatgtt caacattaat ttccataatc ttcacctatt tctttctttc
    22621 attttggttc gtaggatttc cttgattcgg ctgccgcttc aagactaaat catccgcctt
    22681 aatgaaccaa ttaacggtag ttccttctcg aaaaacataa ttggcaacgg tatccacgat
    22741 tggattgacc aactctacct ctgcatcata agcaaactcg cgtaagggta cattagctgg
    22801 gatactaact tgaatcattc gcccttgtgc agaggatttt aaattatagg ttcgttcttt
    22861 tacctcatta ccaattgttc catcctcatt ttgcttccgt acttctcggc gtaatgcgga
    22921 aaaacgcaac ttcccaaatg ttaaacctga atctacgaca ataccttctg ttaatctcat
    22981 cttcttttcc tcctatgctt tcactaaatc atcaacatac aacacgtaat cagtaaaccc
    23041 acgattctca atctcatagc ctttggcaac caaatgggga ttcacggctt ttagcggttg
    23101 gtcttgatca aaatcctttt caccggcttc cgcagctact accacctcaa tatcatcggc
    23161 ccgttgctta ctggaataca aatgataact gcgaccaatc accgtagtat tccgtccata
    23221 tccttctgtt ataatttctc cttcaccggc ataacttaac tttccaaagg tttcagccat
    23281 atctggcacg acaaatttca attccatctt cttttcctct tttcattgtt agttttggat
    23341 acaaaaatag cgcgactact tttttaggag gcggtcgcgc acgttggttc ttaggattta
    23401 cttgtatcat ctcatcacct tagttccggc gattccagaa ataatagccc gccgtcgcaa
    23461 aaatcaaagt aaaaccaatg aacagtaaaa cattggaatt tttctccccg gtttgaggga
    23521 aggtcttcac tgtgggacta gattcttgag agccacttgg aacagttggt gtttccggtt
    23581 gtttcggcgt acttagtatg gttggaactt cttttggtgt taaggtttga gatttttctt
    23641 tcaaatcttc attgtgtttt ccattgatat tcccatcttt atcgtaattg atttccgcaa
    23701 aagtaaactt ggttccttct ggatattttc cggtatctac ttttttcgca agcacagttt
    23761 tggtgaattc tttatcattt acttcataat caatcttgcc agatttccaa atttctttgt
    23821 ttgtaccatc cggtaataaa gcatacagaa ttgtttcgaa agcttctttt gagccatcca
    23881 gtacatcatg agtaatcgac acatcatcaa acatatccac cacatcacca tgagtaaagg
    23941 tctgagaacc gttttctagg tgggcctttg tttggataga aacatggtgt tccactgtac
    24001 aactgacagt ttgggcttgg ttattcaagc taacatcctt cgctactggc ttatcgcctg
    24061 cttcaaaagc ttccttattt tcatacacat agttgaataa gacaatagtt ttatttaaaa
    24121 cttgttcggc ggttaattta tgctgaattt tccaagttcc tgttttctct ttgttcgcca
    24181 acgttgcgct tgtttctgca atcaccactg gtttggcttt ttcatctttt tcttgggcag
    24241 cttttgtcgc atccacatca atggcttgcg ctaccacata ccagtcttct ttgatttcat
    24301 gtagattgta actcaattta tcaactagct cggtagaatc cagagaagtt aattctttgt
    24361 ttccgtcttt ccaagtcgca agagaagtta aactatcttc ttcctcaggc aaatcataga
    24421 gcattaaacg gttcaaacta acagaaaatg ctttgttcgt cagcttctcg taaggaacgg
    24481 tcaccgtttt aattggttgt ttttggtctt gttcggtaat ggtaaagaca tactcactct
    24541 tcacaaagtc ttctttgttt tccttaaaag tagaacggat ttctaatggc gtaatctttt
    24601 gaaaaccttc tggggcttct acttcttcaa gtaaataatc cccataagga agattttcaa
    24661 acttgccata accatcaaac cctaattgct cgttataagc tgtggtcgct tcatcttcgg
    24721 cacctgtgat ttcattggtc ccttccaatg gcgacacttt aaaggtcaga tcattgaagc
    24781 cagtttcggt agtgcccgca gctgatccag caaatttaaa gaaatcaaag ccaaagcgaa
    24841 tcacttgttc ttttgccgta acatttcggg taatcacggc atttttttcg ctatcatcca
    24901 cctttttgat ggatacagga tactttgttt catcaaggct atatccttcg ggcgcttttg
    24961 tttcttgcca ataatactcg ttgatagcta aatgtttaac ggcaacttgg ttcttttcgt
    25021 ctaaagtcaa agtgaccgtt tcatcagaac ctttcgttcc cttcgctaac tctggtttaa
    25081 aagcctcact ccatttaaca gcttttccat ccttagcagt aaacaaggtg tattcagcac
    25141 cctcaaagac tgccttacct tgtgctttat ccccagtatc tttgtcttct ttcgtcaaag
    25201 tggtttcgcc agtgacttct tgattttgcc cttttacgtt actggtaaca ggggctacgg
    25261 tttgattggc atacttcaat tcgactttta caggtttgaa cgtattcacg aacccatgac
    25321 tagctttagt ttcggtcaca taataggttc ccaattccaa cgcattagca atctcttttg
    25381 gtgtttccgc atgaccgttt tcatccgtgg tcatttcttg aacgatttca ccggttggac
    25441 tgtctttgcg aatggcaaac gtattaccag ctaaggaata attgtcattc caaagatcac
    25501 tccctgtttc aactccgctt ttatccagaa tgatttgccc tttttcccgt gcgttttttg
    25561 aagtcacgta aatggtttcg cctgctttaa tggtggtagt catgggggtg gtatcaatcg
    25621 tataaggcgc cggtactgat ttttcggtaa tggttacttt tgtaccatga ggaatctcat
    25681 ccaatgtagc aatgccttct ttgtcagtag tcacgtcttt tgcaggtaaa gtttttccaa
    25741 agtctaaatg gaaaaccgtt cctggtacaa catcacccga ttctttatcg acttttttga
    25801 tctttaaaga accctccgtt tccacatcaa ttttgattgt gtaggtatta ggtttatcaa
    25861 tcgccccggc cattaaagtc tgttggccag cctttttata ggcgacaggt gttccggtac
    25921 cagcagattt cttaagcgtc agcacaccac tttcttttga attggcattt ggggtgatta
    25981 tcaactgatt accattcaca cgataatcaa tgtttgcggc attttccaca acctcatcga
    26041 actctgacaa attcagatta ttcgtatccg tcacagtagt cgattgccct aatactgttt
    26101 tagccgtact attatggaag gttggtttct tctgataatc gtcaatggct tgatttattt
    26161 ttgcctcaat ggctgcgata tttacggcac taccatctga tcgttttatt gagtgaaggg
    26221 tatacccatt aacttcttgc cagatcatct tctgtgccac aatatgagta tctgcatctg
    26281 tacctacttt tttccataaa acggaaacta atttggcttt atcggacata tctggtaacg
    26341 ggtttttctc atagccagga gtgaactcat tgtaaatcgg gactcctggt tcaatacaga
    26401 aaatatcttg tttaaagccg ttgtacactg cataatgcgg aacagtgact tctgacattg
    26461 gacgaccatc actaaaagtt ccttcaacag catattgatg tgctaaatct agttctacgg
    26521 ttaccgtttg tggatgggta atttcatcag caaaagtaat cgtaggagca attgctccaa
    26581 caattgcttg aacagccaaa ctcaaaatag ttaatacaca taatttcttt ttttgttttt
    26641 tatacatgaa atgttctcct ttcaaattaa gcacaaaaaa ataagatgct cgcttgaaca
    26701 tctttgttta taaag
//
LOCUS       DAYYME010000025        26627 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000025 DAYYME010000000
VERSION     DAYYME010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.025"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..716)
                     /locus_tag="ACHBFW_000879"
                     /pseudo
     CDS             complement(18..716)
                     /locus_tag="ACHBFW_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            795..971
                     /locus_tag="ACHBFW_000880"
                     /pseudo
     CDS             795..971
                     /locus_tag="ACHBFW_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1147..1716)
                     /locus_tag="ACHBFW_000881"
     CDS             complement(1147..1716)
                     /locus_tag="ACHBFW_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM9317209.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1790..3079)
                     /locus_tag="ACHBFW_000882"
     CDS             complement(1790..3079)
                     /locus_tag="ACHBFW_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317210.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            3406..4281
                     /locus_tag="ACHBFW_000883"
     CDS             3406..4281
                     /locus_tag="ACHBFW_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9317211.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4295..4618
                     /locus_tag="ACHBFW_000884"
     CDS             4295..4618
                     /locus_tag="ACHBFW_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317212.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4615..5367
                     /locus_tag="ACHBFW_000885"
     CDS             4615..5367
                     /locus_tag="ACHBFW_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFM9317213.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5357..7528
                     /locus_tag="ACHBFW_000886"
                     /pseudo
     CDS             5357..7528
                     /locus_tag="ACHBFW_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7640..9811)
                     /gene="copB"
                     /locus_tag="ACHBFW_000887"
     CDS             complement(7640..9811)
                     /gene="copB"
                     /locus_tag="ACHBFW_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFM9317214.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9831..12017)
                     /locus_tag="ACHBFW_000888"
     CDS             complement(9831..12017)
                     /locus_tag="ACHBFW_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9317215.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="ACHBFW_000889"
     CDS             complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="ACHBFW_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFM9317216.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12239..12679)
                     /locus_tag="ACHBFW_000890"
     CDS             complement(12239..12679)
                     /locus_tag="ACHBFW_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFM9317217.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQKGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12753..13251)
                     /locus_tag="ACHBFW_000891"
                     /pseudo
     CDS             complement(12753..13251)
                     /locus_tag="ACHBFW_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
     gene            complement(13536..15002)
                     /locus_tag="ACHBFW_000892"
     CDS             complement(13536..15002)
                     /locus_tag="ACHBFW_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM9317218.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(15313..17394)
                     /locus_tag="ACHBFW_000893"
     CDS             complement(15313..17394)
                     /locus_tag="ACHBFW_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9317219.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNELIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(17918..18277)
                     /locus_tag="ACHBFW_000894"
     CDS             complement(17918..18277)
                     /locus_tag="ACHBFW_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFM9317220.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     AFLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(18307..18648)
                     /locus_tag="ACHBFW_000895"
     CDS             complement(18307..18648)
                     /locus_tag="ACHBFW_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317221.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVGQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(18645..19319)
                     /locus_tag="ACHBFW_000896"
     CDS             complement(18645..19319)
                     /locus_tag="ACHBFW_000896"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM9317222.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWREDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(20253..21551)
                     /gene="asnS"
                     /locus_tag="ACHBFW_000897"
     CDS             complement(20253..21551)
                     /gene="asnS"
                     /locus_tag="ACHBFW_000897"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFM9317223.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(21591..22781)
                     /locus_tag="ACHBFW_000898"
     CDS             complement(21591..22781)
                     /locus_tag="ACHBFW_000898"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM9317224.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(22802..23329)
                     /locus_tag="ACHBFW_000899"
     CDS             complement(22802..23329)
                     /locus_tag="ACHBFW_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFM9317225.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(23376..26165)
                     /locus_tag="ACHBFW_000900"
     CDS             complement(23376..26165)
                     /locus_tag="ACHBFW_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFM9317226.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIEAGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSSHTGMDTSRFIYHVLEEMKENPEPLDLSLEIIDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIMEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPGQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADVAHRGTGFLARNQSFYKQDFVRFLQKKIKQSNFLIVNHAFLVQETQRKEP
                     LLPASPYLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICVAEGLMNLVSSKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAMDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLLDFQAAMLPSTVWYPRYDHILYIGGTLKIGP
                     NRQYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLEAALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAIDEI
                     YQDIHEFLKKNEEGS"
     gene            complement(26312..26512)
                     /locus_tag="ACHBFW_000901"
     CDS             complement(26312..26512)
                     /locus_tag="ACHBFW_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9317227.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
BASE COUNT         7461 a         5655 c         4689 g         8822 t
ORIGIN      
        1 aaagaggggg ggatttttta tcgttgtgga atatttaaat accaacgctt gtcatgaaaa
       61 tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat catgacgtat
      121 actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac atttcctaaa
      181 gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac tctagctagt
      241 tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt agcaatgtgc
      301 catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat gtgggcgtaa
      361 tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc aattactggt
      421 ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc ataggctagt
      481 ttttctttac ttacacccat ttcagaaaga taaccgtaag gatctgtatg atcgccccaa
      541 atattttgtg ttacccataa atgcgatttg attcctggtt ggttataagg agtgtctaat
      601 gttaatggaa taccatattt cattgctgaa tctcttgcca attcaacgta tactttatag
      661 ttcttttcaa aagtttcttt atcatgtgtg tgttgtaact cgatttgcac aggacttttg
      721 gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg aaaacaccaa
      781 cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta gaagatatca
      841 aagatttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat atttctcccg
      901 aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa ataaaaaagc
      961 actcaaattg agtgcttttt taagctcact atcactaaaa ctatcactaa ttttatttat
     1021 aagcgaacat gtttctatca ctgatttttt aaatctagcg aaaaaccctt atcttaaagg
     1081 cgttcgttca attctttggc aaggtcttca aagcctggct tgcctagaag tgcgaacatg
     1141 gttttcttat agctcatgta gaaaaatttt cttgatgatg attttatcca tcaattgaga
     1201 ttgactgttt tgctctccta tgatttcagc tatgattgtc cgattttgcg gttttcttct
     1261 taactggtag ttgattttcc cagtttccat caactcgcta agcatatcca cgactccgcc
     1321 aaatccggtc tttttcgtaa taaaacctag tgctaccatt cgttcatcta gattttttgc
     1381 tgtaatcgtc acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct cttccatccc
     1441 ttcagcattg gatacgacgg aagctgtttt gttaataggt tcttcgttta tttcttttgg
     1501 ttctttcctc tccactttca ctgtttgacg aaggacatct agttcttgca agagcgtttt
     1561 gtattgttct tgggtcagca tcacgccagc tactttttca cgattaaata tatatacgcc
     1621 agttcccgcg tctcgcgctt gagcaaatac atccatcggc gatcgtttca cttctgtgat
     1681 cgaagatgtg ggaacttcta atttctttaa ttccaatgga tttcctcctt tactaatcgc
     1741 tttttattat agcacagact gacttgtttt gatctttttt ccaaaggttt tatcggtttt
     1801 tgcctaacca gtaattgata tcggtaagaa gtctttcgat accaaatgaa ggatggtaca
     1861 gatacgtttt ttgatcttcg ttatggtcta gtggaaaaaa atgatcggtg atcactagat
     1921 ctgcataccg aggcggctta tttcgagtac atacatgtat agagatccgt tttcgatgtc
     1981 ttagcgtttt gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca tagcgaaaaa
     2041 aaaagacgat tttcacttca ggagggtgga catctaaata ccctcgttcc tggagtgttt
     2101 tcagtaaaag catcccccac tctactgcgg ggtgtgcaag tgcttgttgg atcgctagcc
     2161 gaaattgata tagtttatct ttggaattgg gaaatttttc taatgtttcc tgataataac
     2221 gaacttcttc tatcagccgg tcatggatac caattacgta acgaagccat tcttggtaaa
     2281 gtacttttat cagctttgat ttgctttcgc tctcaaattc gctattcagc caaaacagcg
     2341 aatcaagctt ttcagtcaca atcgtcaatt tccgatagtc ttgtgtctct tctatccgtt
     2401 tccaccgctt ttcagtaatc tggttacagc tcaagcgcaa aattgctaga taattttttt
     2461 cttttattgg caaaatctgc tcctctctaa atagcaattc aggaaacaat tcaaagaata
     2521 gtgttcgact gtctcctaaa atctcgattt ctgccggatc ctttctttgc ttcaataatt
     2581 ggatgaacat ctcagaaaag gactgctctc catcatctgc aaaaaaatgc ggaaaatctt
     2641 tcaaaaagta aaacagcaga tatcttactt ttctttctga accaaagatc tgtgcttttt
     2701 tataagaaaa agaaaggtgg tactcgccaa accactttct caatcgctct tgcgtagaac
     2761 gatcgtactt ctgctcgtaa ggttcattta atagcagatt tttcagtaaa caaaacgggg
     2821 gtgattggat cagaaaaaca aacaagagat aaggttcgct atatccttga tcataaacta
     2881 actgaacggt tttcttatga agaatcagct gaatgggaca gtcgctttcc gccaaacgtt
     2941 tcagatgccg atccactgtt ttaacatcga ttttcagttc ttcagctaaa tcagcttttc
     3001 gccagatcaa atctttcatt agattgatta gtgaaaataa attctgtgca ttttttgata
     3061 atagtttttg ccatctcatc gatcatttct cacctgattt ctaaacatat tgtttgatca
     3121 aaatgagttc actcctaaaa agataaaaaa ctccctttct atttatttaa atacgcaaat
     3181 cttcctcatt attttttatt ttactcaatt taatcgtatt ctcatttcaa aacgaatgta
     3241 ctttaaaaaa acataataag atcactttgc ttgtaggatg tattcccttc cattacaatg
     3301 aaataaatag cgattttatg agaaggtgat gtgaaaatgg aagaaaaaat agaagaatcc
     3361 aaacaaaaaa tcgatcagtt gatataagaa ttagaaaaag cggtcatcgg caaacgttca
     3421 actatccaat tgatgatcgt ggccctatta gcaggtggtc atgtcttatt cgaagatatt
     3481 cccggtgtag gtaaaacgtt actggtcaaa gcactagcaa aagctgtaca gggaaccttc
     3541 agcagagtcc aatgtactcc tgatctgctg cctagtgata ttcttggttt ttccgtatac
     3601 aattcacaat caaaagagtt tgaatttcgt ccgggaccga tctttaccac gatcttgttg
     3661 gcagatgaga tcaatcgtac gactcctcgt actcagtcag cacttttaga agcaatggcc
     3721 gagaatcatg caacgatcga caacaataca taccctctag acaatcattt ttttgtctta
     3781 gctacgcaaa atccaatcga gtacgaagga acatatcctt tgccggaagc acagctcgat
     3841 cgttttttat tccgactaca aatcggttat ccttcttttg atgacgaact tttgctactt
     3901 ttggataaat tcgaaaaagt gttggataac ttaaatgaag tattgaacag ttatgaaata
     3961 gaagaaataa aaacaaatgt ggcgaatgtc tttgtcagtc ccgaagtggc atcttatgcc
     4021 ttgcagttag tgtcagcaac aagaagtcat gctgccatcc agcttggcat cagtccacga
     4081 ggaagccttt cctttattca ggcagcaaaa gcttatgctt taatccatgg gagaaattac
     4141 gtcacgccca aagatttgca ggatttagtt ccttacgtgt tttcccatcg cctgattttt
     4201 tacgatcgtt cattgaagga agacgaaaag aaacaaatca ttcatactct tgttgcacaa
     4261 attcctattc ctgtgaggta gattgaggta gacaatggaa aaaatcaaag attatttcat
     4321 taattttgtg ttatttctgg tatatttcat cattgtgctt tttgctcttg tattcaacaa
     4381 ttctttaggc tggttcttgt tggccttttt tactctttat gtatcatgga gcttgatttc
     4441 attaagcagc tttctaaaga aactttcggt tacttgtaca ttgcagccac tcgtttatcg
     4501 caaaaaaagt atgcaggtta ttttttccat agcaaaaaag agaacttttt ctttacctct
     4561 ccctcgttta caaatcggct tcccagcaat ctttgcagaa aaaaacaaga gattttgatt
     4621 cttacaaata aaccgcaaga agtccaattg ctttggcagc caaaagaaag aggatgccat
     4681 gaaacattaa ctgtcatttt tacagcctat gatagtctag gacttttcag aaaaagtgca
     4741 aaaagagaga tctcttttcc tgtgactgtt ctgcctgctt tttgtaagat tcaagcagaa
     4801 aaattacaga gagttctgga aaaaaaacag cagctgaacg cgtaccaaag aggtcttgat
     4861 tttcgggaac atcgcgctta tcgtccaggt gattctttca accgaatcga ttggaaattg
     4921 tctgctcata ctcaagagtg gttataccgt gaatacgatt atcaagaaga agaatgttct
     4981 cctctttttt gcttttgggg ttcgcccagt ccaaaatttg aacctctgct agacttgtat
     5041 ttttctttat ggcatttgag aaaagaaaag cagccggaac ttctgatatt aggcaaccgc
     5101 gcctttcatg gcaaagaccc cggtcatacc tattttgctt ctttggaagc tggcgatgag
     5161 aaagcttttt tcgcacactt gaaaaaatat gcaaaaaaac aaatcgtcct ctttgttcct
     5221 gagaattctc ctgaagtctc agaagctatt gaaacattgt caaaagatcg aacggcttat
     5281 cttgtttatt tcgtagaaaa acaactcatg gtccaagaaa aagacaagat atgttcattg
     5341 ccaggaggtg aatggattga cgattaaacg ggaaaaatgg ctgcgtggcg tttactcctt
     5401 tgtgatacta agtgtaggtg ctccgatatt tgtagtagct aatcaattgc aaaacagcta
     5461 ccttcttatc ctaactattg gtgtcctatg ccttattgtc tgctgtattc ctaaaaaatg
     5521 ggtacgtttg ctgctttctt tgccagtaat gataggttgt ttgtttttat attttccttc
     5581 gaagacattt tccatattat ggatcttaca gtttttttcg tcagttacag aggaattctt
     5641 ccatctatcc caaggtaaac tttattattt gccagaaaag acagcttttt ttctcataat
     5701 gttgctgatc tatttattta ttacattgac cgtggattac gactattggt acgcaccctt
     5761 tctttctttg atggcttact tcatgatgct taccgttttt cggaaaaaag atttactgta
     5821 cgaaatgatt gcggtagtaa ctgttttgtt cgtgtatctg gcagcaaggc aatttttctc
     5881 catccggttg ttgtcgggaa acactcgatt ccagtcattg actacagttc tgcttatttg
     5941 tttcttaact tttagcgcta tcttgccttt atatcttccg aaagtacacc aatctctcga
     6001 ggaaacgagt gaaccgatcc gagaatatct taatgatgaa gggctttatg acacactcca
     6061 tgagtatcaa ttaaccggaa gtgcaagaac aggcttcagt gagaatgatg aacagcttgg
     6121 tggaccttta tatgataatg gcgaagttgt ttttacagcg aaccaaaaag gaaatggcta
     6181 ttggaagatc gaaaataaaa atcattacac cggttctgga tggatagaag atcaggacgc
     6241 acccatccaa ccaatccaag aattccctgt tgacatcacg agctaccaaa ccgttataga
     6301 agacaaagaa aacattacat tgaccctaaa aaaacaagct ttcttacctt atccttatgg
     6361 aaatactacc ttgactgatt tttcattagc tagtggacaa acgctttact atttgcctga
     6421 aagccagcga ttcattcctg tcccgaatga atcgacacgt tctgcaactt tacaaacaga
     6481 aaggttagtt tttacgagag aagaattaaa aagcagtcag ttaaaagctg atgatttaga
     6541 aaatcagcag acaaatcttc aattgccaaa aagtttgcct aaacgtattg aggacttggc
     6601 ggtcgaattg accaaaaatg aagcaacttt aattcgtaaa gtcgaagctg tctaacagta
     6661 tttaaaaaca agcggaaatt tccgctattc caaaacggat accgggcata ctccgaaaga
     6721 acacgattat gtggattatt tcctctttga ttcaaaagtc ggctattgtg acaacttttc
     6781 aacttctatg gtcgtccttc ttcgaacact tggtatccca gctcgctgga caaaaggttt
     6841 tacaaatggg ctcagaacag gcacagataa tcaagggaat gaggaataca gcattctcaa
     6901 tagtcatgct cattcttggc cagaggtata ctttcctgga tttggctggc ttccatttga
     6961 accaacccct tcatttgcca atccaatggg ttcacaacca gttgctgaaa caaccgaaac
     7021 cgtccagcct gctatcgaac aaccaaatga aagcgaaaat atggcagaaa ctatttccag
     7081 tgagagatct gaggtcattc catcagaaga aacaacactc acaccagctt ataaaatcgt
     7141 cttattcagc cttggagtgt gtttatccgt cttgttgttc tttctattgt tattcagaaa
     7201 atctctgcgc tggacaagtt atcttatcta tcttcatttt tttgcttcta ctaatacgat
     7261 tgcatatcaa aaacttctta accctttgga aacattggta ccgcgcaaac ccgcagaatc
     7321 tttgcaggaa tatagcctgc gaatagaaca agaattcaag ctagatctgc gtttcattcg
     7381 attaactact caatatgaag cagaaatata tggacgacaa gcagatccgg taaaattcga
     7441 taaaaaaacc tatttagaaa taacgcgtat aataagaaaa acggtataca aaaaaattct
     7501 gacaaaaggg agccgcttca aaaaataaga taaaatttct ggagaatcta cttctggaat
     7561 gccgagaacc agctgattga gtatcgatta ctcgaaaaaa aggttgtgac acgttttttg
     7621 tcacaacctt ttttcattat tatttgattt tcaatgtcaa agcatttaga gctacaacta
     7681 ccgtacttaa cgacatcaag acagcaccaa ctgctggact taatatgata ccaattggcg
     7741 caagaattcc ggctgctagc ggaattgcaa gaatattgta acctgccccc caccagaggt
     7801 tctggatcat tttctttcgc gtttgcttgg ctagatcaag aaaatgcaaa atatcttttg
     7861 gatcgctgtt tgtcaatacg acatccgcag aatcgattgc gatatctgtc cctgcaccga
     7921 ttgccatccc gattgaagca cgggctaaac ttggcgcatc attgatccca tcacccacca
     7981 tgattacttt tttcccttgg tcaaggtatt tctggacaac agcttcttta tcatctggta
     8041 ataaaccgcc ataatattct tcgatcccta aataatctgc tactgcttgg gcagcgtttt
     8101 tattgtcacc agtaagcatg acaggagtga tttttctttc tttcaatgtt cggataaatt
     8161 cttttgcttc tgtttttact acgtcaccta aagcaacgat tgcagctaga tgattttcta
     8221 aaatcaggaa gctgatggta tttccttgtt cttggtaagg tttcaataat gcttgttcta
     8281 cgtttaatcc gagacgctca acttcttttt cattaactat tttcacttgt tgttttttga
     8341 ctgttgcctc tagcccgact cctgaaagat tttttagatt ttcagcttga taaggcttga
     8401 tttcatgttc tttcaaataa ttcataatgc caacagccaa tggatgattg gcattcgctt
     8461 ccaaggctcc tagatattga aggatttcat ttttattgaa cgcatcatca agtacttcga
     8521 cacccgttac agtaaatttt ccttctgtta gtgttcctgt tttgtccagc atgatcacat
     8581 ctagatcatt ggcttgctcc agtgcattgc ggtttttcaa aagtaatccg tttttagctg
     8641 ctattgatgt ggagcgagca acaactaatg ggatagctaa acctagagca tgaggacagg
     8701 cgatgataaa gactgttacc atacgttcta atgcattagg aagatctgtc aagaacaacc
     8761 aggcgatgaa tgccaggata cccacaatta atgcgacata aaacagccat tttgccactt
     8821 tgtcagataa tgactctagc ttggattttt ctccttgtgc ttttcgcacc atttccataa
     8881 ccttcgctag ataaccattt tcaccagttc cagttacttc gatttcgatc gttccatccc
     8941 cattgataga accgccaatc actttgtcat tggcttgttt tttgattcct cttgattcgc
     9001 cagtcacagc tgattcgtca acaattgttt cacctttcaa gattacacca tctgttggca
     9061 ttttatcacc agaccggacg atcaaacgat caccttcgtt tacttctttc aatgaaactg
     9121 tctcttcttt tccatctttc gtcaagcgtt tcactgattc tggcagtaat tctgctagtt
     9181 tttgtaaagc attgctggca ttcgacacag cattcatttc aatccagtga cctagtagca
     9241 taataacgat caaagtagcc aattcccaga agaaatccat gacgtgtgta tgtggattga
     9301 gcagatttgc gataaacgaa tagacactat aaatatatgc aactgtaatc cccatcgcga
     9361 tcagtgtcat catagcagga cttttttgtt tcaattccat cttagctcca cttaaaaatg
     9421 gctgaccgcc atagataaat agtatagttg ctaagatcaa tacgagccaa tcagaaccag
     9481 gaaacgttac ttgaaatgga aattccattc ccatcattgg tgaaaaaaga atgattggga
     9541 tagctaggat aagcgatagc caaaattttt gtttgaaatt ccccatgtgc atggaatgat
     9601 ccatgccgcc gtgttcgtga tccatgtgca tcgaatggtc catttgtttg ctgtttgcta
     9661 tatctccatg gttatgtccc atgtgcatgg aatgatccat ctgactatgg tcatgtttca
     9721 tctccatctg tttttcatga gattctgtac tttctttgtt tactacttct tttttatgct
     9781 catgtttttt ctccatatcg ttcccattca tttttactcc atcccttctc ttactttttc
     9841 atgtgtcgat ttaagctcag ggagttcaac aatacactga cggaactaaa cgccatcgct
     9901 cctcctgcaa tgattggatt caaaaagcct agtgcggcaa aaggaatacc aatcgtatta
     9961 tagacaaaag cccagaaaag attttgtttg atttttctca atgtcaacct agacaactcg
    10021 attgtttgtg caatggatgc caaactgcta ttcatcagcg tcacatcggc tgtttccatc
    10081 gcaatgtccg ttccacttcc cattgccata ccgacatcag ctaatgctaa ggccggtgca
    10141 tcattgattc catcgcctgc cataccgact tttttgccat ctttctgaag tttttctacg
    10201 taggctgctt tatcctctgg aagtacttca gcaaagatat gctttggatc aataccaact
    10261 tgtttaccaa tcgtttcggc tgccagtttg ttgtctcctg tcagcataaa aacatctaat
    10321 cctttattct ggagctgctt gattgcttgt ttggcttcta gtttgatttg atcagccacg
    10381 gcaatccacc cgatcacttg cttttcatca gctaaaaaca tgactgtttt tccctgctgt
    10441 tctaattcta acgcgtgttc ttggtattca tcaaatgata ggttcaattc aattaatcgt
    10501 ttacgtgtac ccgcaaaata gcgtactcca ttaatcgtac cgctgatccc agcacctggg
    10561 tgggctgtaa aatcaatcat atcgtatgtt gctgcttctt gcagttttcc gtattccacg
    10621 attgctttac ctagtggatg ttcagaagct tgttctaaag tataaaaaat agataggact
    10681 tcttttgaac cgactagatc tgtaactttg ggctttcctt ctgtaatcgt tccagtttta
    10741 tccaaaacaa tgctgtctaa gtgagcagct gcttctaatg cttctccgcc tttaattaaa
    10801 attccatttc gtgcaccaac acccgttcct accataatag ctgttggggt cgctaaacct
    10861 aatgcacatg gacacgcaat gactaataca gagacactat ggatcaatgc gagttgccaa
    10921 tcaccagtga tcaaacctgt agctatgagc gtgataaacg cgatgactaa tacgatcgga
    10981 acaaaaatcc ctgagatccg atctgcgatt ttttggattg gggctttgct cccttgagca
    11041 tcttccacca tttgaatgat ttgcgctaat actgtctgct tgccgacttg tgtgacttcc
    11101 atgtgtagca gaccattcgt attgattgtc ccgccaaaaa gcgtgtcatc cggattcttt
    11161 tctacaggaa gattttcacc agtaagcata ctttcatcta tcgcactgct tcctgaaata
    11221 attcgaccat ctgctggaat ctgctcacca ggacggatga ctagttgatc accaacgact
    11281 acttcttcaa tcgctaacgt ttcttctttc ccgtttctta tcacttgggc ggtttttgtt
    11341 tgaagagaca tcaattgttt gattgcatta cctgttttcg tttttgctgt atgttccaaa
    11401 tatttgccga gcaaaatcag cgtgatgatc atactgctgc tttcaaaata aagatcttgg
    11461 gggtgtccac ggaaaaatcc gttgtaaatg ctcaatgcaa aggcagccga tgttccaatt
    11521 gctaccaata catccatatt tggtgctttc gtcttgattg catggtacgc acctttataa
    11581 aaacgagcac cgacataaaa ttggacagga gcagacagca ctaactggac gattggaaaa
    11641 tgaaaaaagt gaacaatcgc cgcatggctt cctaacatca tagcaatcat tgacaacatc
    11701 aacggcaaag tcaaaaccgt actcaatata agatcgaata gcattttttt caaatacgct
    11761 tgcttttctt ctgcaatttt ttgtttgtgg gcttcatcgt acaaaattgc accataaccg
    11821 atgttttcta cgctttggat taatttttca gtggtcgtcc cttcatattt gacacttgct
    11881 ttttcagtgg ccaagttgac tgtggcattg accacaccag gttgtccgtt cagctctttt
    11941 tcgatcctag cagaacaatt tgcacaagtc atacctgtaa tgacgaacgt atccattttg
    12001 gtttctgctg ttgccatcaa atcacctctg ctttataacc caatccattg attgcttgac
    12061 aaatcttttc ggattgaact tttgtttcat caaacttgac cgtaccgttt gcttttttca
    12121 aatttacttt cactttttgc acgccgtcaa gtgcgcttac tgtttcttct actcttgctg
    12181 cacaatgatt gcagctcata ccttcgattg aaaattgttg tttcattttt tcttcctcct
    12241 attgatgttg tttacattca cattgacctg ggatacagtt acattcgatc gtatctactg
    12301 gttgcttttg ttccagttgt ttcaaaatct cattgatatc ttcttgagtc aaagtagctt
    12361 cttctactag atccgcaatc gtctctccaa tcctttttgc acagatatgg gaaaaaagat
    12421 tttccgtcgc actgcgaact gtttccatct ccgagacagc tggatgataa atgaattttt
    12481 ttcctttttg ttctgtccag atcacttctt tttttaccaa acgtcccagc aaagttttga
    12541 tcgttgctgg tttccaatcc atcgaatcag ctaagatctg cgtgatttct tgggctgtcg
    12601 tctgtccaag tgtccaaata acacgcatga tctcccactc tgaatcactg atctttactg
    12661 gcgttttgac tttttccatt tatttcacac ctttcgttta caaatgtaat cattagctta
    12721 tgaatacaga ttacgcttgt aaacaaaaac tgtcaattat ttttgctctc tattttcaat
    12781 ttagttaaaa attcttgttt ctctcgtctt gttttaagga tgatcagttt atttcttgca
    12841 ttagctactt ggatcagctg ctgtattttt ggttcattgt ttccttcaaa atgccagata
    12901 aaagataaaa attctaagta tttcctgttt agtttctccg aaaatgattc aggcatatct
    12961 ggccgagatt ttttgtttat ctttcttttt agagaacgcc aaactactcg gtagatggcg
    13021 agataacgcg gcactttaag ccaaatgatc ttatctgctt ctttaatcct ttcctctaac
    13081 gtagaagtat aatttccttc aacgatccat gacggattag atactataaa cgattgctgt
    13141 tcttgcaaaa accacttttt tgcctgaatg cggaaagccg gtgatttctt tgatcttcct
    13201 tgcgagggac gattttcccg acccactttg tccaatcacg atgatcttca ttcttctccc
    13261 tcacttctct ttggaatttg gttcgatcat accacttata acgccgcaaa aaaacaaggg
    13321 actgtgacaa aagtcgtgtc aaagtccctt gtttcgaata aatggtgttc aatcagctga
    13381 ccttcagcat tagctcaaaa aacggcaaaa tatgaggagc tatttttgtt tggctgttct
    13441 tattacttga tgcaggacag ctttttcaca acctctgatt cacggtgttc cagaagtagc
    13501 ttctgtcaca acctcttgta acaagtattt ctattttaag gctgaaccac tgtttctggt
    13561 tcagtaattt ctttgttttt gtctcgacga gcaattgctg gcaagatcat acctaataag
    13621 atcaagacaa ttggtgtgat gatattcgtg aataattgga accaccatgc tttaggatca
    13681 gcagtataat ctacttttgg caccattcct aggatacatg caaacgcagt gaaggcaaag
    13741 caccaagcac caaagataaa accgattttt ggatttttaa caaatttata ttcagaattg
    13801 aatttcttat aagctttatt caacatcata tatgccaaga acacccatag gtaacgcatt
    13861 ggcatgacaa ctgagttcaa gttcgtcaac cattttacta attcattcat atctttgata
    13921 ccaaagatcg gcaacaaaat aatgattcct actagtatac ctgtcagaag ataaccattc
    13981 accaatgtac ctttacgatt acgtttacgt agccagtttg gtacaaattc tggatccgca
    14041 tctgctaaca agatttttag tggtgcatca attgaaaagg ctaatgcgga gatttgtcca
    14101 atcatgtttg tcaatgcgta aatgatcatc aatgagttac cgataccata gtaattacct
    14161 aaaatttgaa aagcactgta tgccccattt gccatcaagt ctttagggat attatcgcta
    14221 gcaaacagca tacccattgc gactgagcct aatacagcac aaatccctac cattgctgct
    14281 aagaagatca tccctttagg aaattccttt gcaggatttc ttgtagagtt gacataaggg
    14341 gatatttttt ccgcccctcc aactgcaaat actagcatcg aaacggttgt gaaataactg
    14401 aaatcaaatg ttggaatata tgttttcact tgattcatat gtggtgtggc aaattccatc
    14461 gttggattga tgaacggtgc cgttacagct aaaagaataa ataagatcga catgacgaac
    14521 atcgctgttc cggccagtcc gccaatcact ttcaaagtag aaagtccttt tgttgataaa
    14581 tataaaaagg caagaaatat aataagtgaa ataactgcta ccatttgtac tgacatcgta
    14641 tttacgatat ttccatttcc ttggactgcc caaccaccag caatcagaat tgcttgaggc
    14701 ttttgtgcca aataaggaat atggacgacc cagtaagtcc aagcggcata gtatgccagt
    14761 cgtttattag atgtcgtatt ttcgacccat gaactgacgc ctcctttact atccttgaag
    14821 gctgatccta attgtccaac gatcaatgcg tatggaataa aataaatggc caaaatcaat
    14881 agccaagagg tgacaactga tactccttgc tgagcatagt tattaactac attccccatg
    14941 ccccaaacca tgttaaatgc aattaacgca acagtaaacc agcgtaattg tttgttgttc
    15001 attcgttctc tcctttatga taaaaatttg ttatccagat gtaatcatat tcaattttta
    15061 acaaacttac aagacagtgt tattttatgt gtgtattatt ttatttaata cacatcgttt
    15121 tttcatattt tttccatcaa aaaaataagt tattttcttt tatttcattg accttattga
    15181 atttcaagct attttgaatt ttctgcaaaa ttaaaaacat tagttatttt ataatcctat
    15241 ctattttctt tacatttttc attaagaaaa ggatataaaa aaaagaggac gttcattcgt
    15301 cctcttcatt gatcaacgtt tattttttac atgcaagctt cgcatggcat tcaaaacagc
    15361 taatactgtc accccaacat ctgcaaatac tgcatctccc attgatgcaa aaccaaaagc
    15421 acctaaggaa agtaccaaaa tttttatccc aatagcaaag acaatgtttt gtttgacgat
    15481 tttcaatgtt ttctttgcta aatggattgc ttctgcaatc ttcgatggtt gatcattcat
    15541 gatgactaca tctgctgctt caattgcagc atcgcttccc aatccaccca ttgcgattcc
    15601 tacatctgct cttgctagca ccggtgcatc gttgattcca tctccgacaa atgcggtctt
    15661 tttattgtta ctttgtaaga tttcctctaa ccgctgcact ttatcttctg gaagcaattc
    15721 gctatatatc ttgtccacgc ctacttgctt cccgatatga tcagcaattt ttttcgagtc
    15781 gcccgtcaac atgactgtgt ttttcacacc agcctgtttt aattgaacca atgcgtctac
    15841 ggcatcttct ttcaaggtat ctgcaataac aagataacca ctgtaacttt gatcgattgc
    15901 tagataaagc aatgtaccga tttcttgact tgaaggaaaa gagatttcaa atctttccat
    15961 caatttgtga ttacctacta atacttgttt gccatcaact tcagctaaga tcccttcacc
    16021 tgcaaattcc tggatattag ctgttggtaa aagcggacct gcgtagcctt ctaaaacaga
    16081 ttgagcaatc ggatgtgtag aaaactgttc tgcactcgca atatattgca tgaatatctt
    16141 tggatcgatt tttgtatcga tcgtctggat cgaaaagtcg cctttcgtta atgtgccggt
    16201 tttatcgaat accactgtct cggtttgcgc aagcaactcc aagtaatttg aaccttttac
    16261 taatacaccg attttacttg ctcctccgat accgccaaag aaactaagtg gcacggaaat
    16321 cactaatgca catggacatg agatcactaa aaatgtcaat gctcgataga tccattcaga
    16381 aaacgccgct ccagaaacaa caagtggagg taaaacagct aataaaacgg ccaatcctac
    16441 aacgactggt gtgtaatagc gcgcgaattt agtaatgaaa ttttcagctg gtgctttttt
    16501 actgctggca ttttctacta attctaataa tttgctgata gtagaatttt cataagattt
    16561 ttctacttga actaaaaatg gtttattttt attgataaat ccgcttaata ctgtttcacc
    16621 aacatataca gatttaggga ctgattctcc tgtaagtgct gacgtatcga ctaatgattc
    16681 tccttcgatg atcgttccat ctaacggtac acgttcacca ggcttcacaa gtacaaactg
    16741 accgatcagc acgtcttcag ggttcacggt catcaatcct tccgccgtct gcacgtgagc
    16801 tgtttctgga cggatcgcca tcaattcgcg aatcgatttt cttgaatgac tgactgccaa
    16861 tccttggaaa tactcgccga tttgataaaa tagcatcact gctacggctt ctggatattc
    16921 accgatcaac atagcgccaa tcgtagcaac agacataaga aaattctcat caaagatttt
    16981 tcctttgacg atattcatca ctgccttctt cactacatca tatcctatca gcagataaat
    17041 caataaatac cctatcaagc gaattagttc attaaaatca aataatagca ataaagcgat
    17101 acctgcaagg gaaaataaaa tccggaacag cgtatttctc tctttttcat ttccatgttc
    17161 agatactttt tccttatcta tttctgttac tttgacatct ggttctaatt ccttcactac
    17221 tttttttgtt tcttgttcaa tcgtagccaa atgatcttcc tccgcgtcaa tcgttagttt
    17281 tccagaagtg aagtttaccg ttgcttcctt gactccattg attttctgaa ctcctttttc
    17341 gattttcatt gcacagttcg cacaatctaa accttctaat ctgtaacttt tcattttaat
    17401 gacctcctcg cctcaacata tgaatatctc ttcataacta attataaatg aatggttatt
    17461 catatgtcaa ggtatccgaa agaaaaaatg aaaaacatct gcatcggaaa atttcttcca
    17521 tacagatgtt ttggcttatt ctttcttttt ctcttcgatc attgccactg ctcttatcca
    17581 acccgcactt gccttcttga tcttgacagg atagcaacta agtttgaagc ctgttgctgg
    17641 caatttgtct aaattcatta atttttcgat aggacaatat cttttttcaa tccctgcaaa
    17701 atgtccttcc cagatatacg aagctccact acgctttcaa aaatatagaa agagttcttt
    17761 aatgaaaatt atttcaactt tccataaaca gtaaccccat ataaataaaa gacgtaaaag
    17821 tcacatggaa gaacttatac aggaaaacgc atagagtcag gtgaaataca tgtatcccac
    17881 ctgactctat gctttatcta acctttatta ttctctttta tacaatttct gcaccgaatg
    17941 gcatcaacgc caatcgagca agtttgaaag attgcttggc aaaaggtatc cctatgatcg
    18001 tgatacatag aaggatgccg ctgacgacat gtgcgagagc aattggtagg cctgaaacga
    18061 tcagccagat gatattaacg atcaatgaca tgccagactg gtgataaatt acttctttgc
    18121 caaaaggcgc aaaagaaagg aaggcgatct taaaacattg cataccgatt gggataccta
    18181 tgatcgtgat acaccaaagt atccccgctg ctaaccagct aagaccaccg attagtccgc
    18241 cgaatataaa ccagatgaca tttcctaaac aactcattgt atttctcctt ctattactgt
    18301 ttttgattaa aactgggcaa aaattgattt tattcttctt tttttagctt ccagcatcaa
    18361 atattctgca aatttttttt ggatttcttc cgtattttct tttgttacta atgtgtgttg
    18421 gattagtttt cctgatggtc gctgacccac ccgttcttct gactgttgca cgtccgcttg
    18481 cgcaaaaccg ataatccgtt tttcttttgt tacagcaacg agcaatagtt tattcttatc
    18541 atttaggtca gctgccgttt ttgcttttac gaatgattct ggatgatcag taatcagctg
    18601 attgatttga ggtagatctt cttttactgc ccaccgtatt tccatcattt ctcacttttc
    18661 tttcgctcat ttttatctcc actaaaaaaa tcgactgcgg caattgcatc tatccagctt
    18721 tgtgcaatga tctcatgtcc gccaatcgtt gggtggaccc catcttctct tgtatatttc
    18781 gaaaaaccat aagcaatccc taaagcattg aatatgccat ctaaaggaac cagcaatgcg
    18841 ccatattcct tagcaagttt acgaacaatt tggattttag ggtctaagtc tttacgccag
    18901 ccatgacggt cgtcggttat aggaagtaca aacggctcca tcaagatgat gttgcagatt
    18961 cccgcttttt gaagttcatt tagtaatttt cgataaacgg attcaaattg ctgagcagat
    19021 ttctcagttc cgaatatagc ctgatcgcta gtattatgcc aggtatcatt gattccgatc
    19081 aaaatcgata catagtcagg ttgtaaagct aaacaatctt ctcgccagcg tacttgcaga
    19141 tctgcaattt tgtctcctcc tatcccacgg ttcatgcatt caaattccat cgttggatat
    19201 tgggagatta gttttccggc aatcaaaagt ggataacctt tacctagatc agttgcttct
    19261 gctcggtctc ttcctgcgtc tgtaatgctg tcaccgataa ataatatacg ttttttcatt
    19321 tgtcaaaaac tcctttttga ataaaacgat gattttcttc cttatattat cccataagcc
    19381 ctatcaagac gaaagagaaa atatatgtat aaaaaatgac accagcctag tcccttcaaa
    19441 caattgaatg aacgctcatc aagtgtctag ttttttatgc tatttttttc ttaacgtgct
    19501 ggattcccct tgattccttc attgtttggt ccagagagat cgatcacacc taagcttccg
    19561 tcaatcagac cacttgatga cgccatcaga acatatattt ataggctcat ctaagacttc
    19621 cagtaaatgt tcctttacta atatctcttc tagattgctt gggatagatt ggccataact
    19681 ttatctggtc cgatgatttc aagggtgaat tgaacttaca tatggatcat tgcatgctga
    19741 aaatacggac ctactccatc ttcgaatgtt actattagtt tgttttcagg tgctagatgc
    19801 tgctttattt caacagcaga agcatcatca aatcagattt ccatttgaat aaccttcttt
    19861 gtatatgttt gattgattgc gatttcgtta tagaaccttg tgaatcaaat agcaatttct
    19921 tttgctttct tctcttatct gctggaaact tcctttatcc cgtatttaaa aaaaaagaac
    19981 tgcgagagtt cgcagttctt ttttttaaat aaacgttgct ctccggccaa ctccttggtc
    20041 acaagtcaca atagtcatca aacatgaaaa aatgtttttt tgaaattact ccttgtgact
    20101 cggagctgaa cggcagacgc acaacctctt gatataaacg gtgttcaatc agctgaccct
    20161 cgatattagc tcaaaaaaca gtaaaatatg agaagctatt tttccgtttt gtttctctaa
    20221 tactcaggtc aaggcactga tttcacaacc tcttatggat aaattcggtt caacaaacgt
    20281 gggaatggaa tagcttcacg cacatggtcg ataccagcaa tccaagtgac tgttcgttca
    20341 aggcctagac caaaaccaga gtgcggaaca gaaccgtatt tacgtagatc aagataccat
    20401 gaatattctt tttcatccaa accgtgatta cggatttgtt ctagcaagaa atcatagtct
    20461 gttgcccgtt cgcttccacc aataatttca ccgtaacctt ctggtgcgat catatctgca
    20521 cagatcacca cgtcttctct tgttggatgt ggtttcatgt agaatggctt gatggctttt
    20581 ggatagttta ggataaagac aggctgttcg aatgaattcg caatgaacgt ttcatgaggt
    20641 gaaccaaagt cttcgcccca ttcgatgtca tcaaatccat ttttcttcag taattcgatc
    20701 gcatcatcat aagtaatacg agggaatggc aattttgtat aagtttttaa gacttctttg
    20761 tctcgtccta gtacatctaa tgcgtattcg caatgatcta aaacattttg tactaagaat
    20821 gctacatatt gttcctgtac ttctaaactt tcttcttgat gagtgaaagc catttctggt
    20881 tcgatcatcc agaattcgat caaatgacgt cgtgtttttg atttttcagc acggaaagtc
    20941 ggaccgaatg aaaagacttt tccgaaagcc atagcagcag cttccatgta taattggcca
    21001 ctttgagata aataagcttt ttgatcaaag taatttgttt cgaatagatc cgtagtgcct
    21061 tctggtgcag aaccagtcaa gatcggcgga tcgattttga caaaatgatg ttgattaaag
    21121 aattcatacg ttgcacgaat gatttcattt ctgatctgca taatcgcatg ttgtcttgaa
    21181 gaacgcagcc ataaatgacg atgatccatc aagaagtctg ttccgtgttc ttttggtgtg
    21241 atcggataat cgtggctctc gcttaccact tccacatttg taattccgat ttcataacca
    21301 aattttgaac gactgtcttc acggatttct ccagttacta acacggatgt ttcttggtgt
    21361 agatttttag ctgtttggaa aacttcttcc ggcacttcgc tttttacaac tacaccttgg
    21421 aaaaaggctg ttccgtctct taattgcaaa aatgcgattt ttccgcttga tcgcttattt
    21481 gcgacccaag cgccgattgt tactgtttct cccacatggt ttttcgaatc gatgatttga
    21541 atttgttcca cgtatgactc tccttttata ttccttgaat ttcttcagga ttaattttga
    21601 cttttactct caataaattg cttgattctg cgtaccgctt cttctaacgt agcgcgatca
    21661 gtggcataac tcatgcggac attttctggt gccccaaagc cttcgccagt cacaagcgct
    21721 acccctgttt cttctaaaag atcttctacc cattgtgtga cattttcata tttacacatg
    21781 cgtagcgttt cttttacatt aggaaaaaga taaaaagctc cttgtggctt ttcaagagaa
    21841 acacccggca gttctgccac aagcggatat aacgtattca ggcgttcttc aaaggcgttc
    21901 cgcatttctt ccactgtatc ttgctcgcct ttcaatgctt caacagctgc atattgactg
    21961 actgctgtcg gattactggt tgactgggaa gcaacagcaa tcattccatt gatgatctca
    22021 gaattcccca ccgcataacc aatgcgccag cctgtcatcg catatgtttt agacacgcca
    22081 ttgatgatga tggtctgatt tttgattgct tctgaaatcg ttgcaattgg agtaaactca
    22141 ttgccattat aaactaaacg gccataaata tcatcagcaa ctatcaaaat atcatttttt
    22201 actgcccatt caccgattgc ctgcagttca ttcttgctat aaatcattcc agtcggattc
    22261 gatggagaat tgatgattac ggcttttgtt ttttctgaac gtgcttgttc tagctgctca
    22321 acagtaactt tccagctatt ggattcttcc cctttcacaa agacaggaag tccttctgcc
    22381 agttttactt gctcaccgta actgacccag taaggcacag gaatgatgac ttcatcttgc
    22441 gggtcaagga tcgtttggaa aagggtatac aaagcaaatt tcgctccgtc tgttacgact
    22501 gtttgtgaag gttcatagac aagtccatag tttttcttca gataatcaac gattgcctgt
    22561 cgaagctctg ggattcctgc tgttggtgta taatagctag ctttcccatt gcggattgct
    22621 tcgattgctg cttcttggat gttttctggt gtcgcaaaat ccggttctcc cacagtcaaa
    22681 ctcagcacat ctctgccttt tgcttttaat gccttggcct ttgccgcagc tgccaaagtg
    22741 acagacggtt ctaatcgttt tgttcttttt gataactcca tcccattcat tcctttacct
    22801 tttaaattcc cttgattgtc ttgacttcat ctccagattc aaagccaatc aggtaataat
    22861 tcagtttccc atttgtttct tttcctacta cttcccacac aggctgatca tcatacatcc
    22921 ctaaagcagc tttttcgatt tggatttcag gatgggccgc agcaattgtc tgttttgctt
    22981 cgttttcagt cagtcccttt gtttgatcca atacttttac tttttcacca gatttaggaa
    23041 tgataacggc gatttctttt cctttatcat tcgtacccgt caatgaaaag tacgttttct
    23101 ttcgagtaaa ccagtaaaaa tgatcggctt cctcgagatt tgcatatttc tttgcgattt
    23161 ctactgcttc ttttttcgcc tggaccgccg gacgactgga acgcaagtaa aaaatcgagc
    23221 tgatcacgat gaccgctaat aaaaatacga tcaagccaag caacacgcga ttaatcgttt
    23281 tttcctttgt ctgttttttc ttcaagggtc gtcgctcctt tatcttcatt ccttttcatt
    23341 atagcagatt ttaatgatga aaaaagacgg cacctttaac ttccttcttc atttttcttt
    23401 aagaattcat ggatatcctg atagatttca tctatagcta gctcttttat cggtactttt
    23461 ttcggtaatg catggatgat tcgtgcacca tattttgcag tgactaatct ccgatccaaa
    23521 ataagtaata cgccacgatc tgtctccgtc cttatcaaac gacctaatgc ttggcgtaac
    23581 ttcaaagctg ctttaggcac ggattcctga taaaaaggat tgatcccctc tgattccaga
    23641 taagcattcc tagctttgac catcggacgt aacgggttgt cgaagggtaa acgtgtgact
    23701 acaacaatct gcaaagcatc tccaggtaaa tctacgcctt cccaaaagct gtcagcacca
    23761 aataagacgc ttttttctga caaaatgaag cgtttcaaaa gtttttcccg actcccgcca
    23821 aatccttgag caagaatttc acgtcctttt tctagcatag acaaatgcat tttctgatat
    23881 accttttgca aaacatcatg agaagtgaat aaaacaagga tcggccgatc ttcttgttca
    23941 atcaatcctg tgagtgctgc ttctaaataa tcgatatatg cttgattgct gacttgatgg
    24001 actgcaggtg cttctttcgg cacatagatt cttgcctgtt tctcataatc ataaggagaa
    24061 gcaatcactt taagaggtgc tgcttcaatg ccaagtcgtt tagagagata ctgtcgatta
    24121 ggtccgatct tcagtgttcc tccaatataa agaatatggt catatcgagg ataccacaca
    24181 gttgacggca acatagctgc ttgaaaatca aggagatgaa aaatcatctg ctgtgctgta
    24241 ttcccgggaa cgaaccaatg gatcaaatga tctcgccagt cttctagcca ttggtccatc
    24301 agcaagtatt gcttgtctaa ttgatcaaat aagctatata aacttgctaa atcgatccgc
    24361 atctggttgg tccaactttc ttggataatc tcaaaagtca agcgcatctg tgattgtagt
    24421 tgttggatct cttgataaaa aagttgcaga tgatccattg ctgttccaga ttccagtggc
    24481 agctgatcca acatttcttt tgtcatcatg acttcttctg gatattgtcc gttaggttgc
    24541 ttgctgctta ctaagttcat taatccctct gccacgcaaa tctgcgcttc gcatagtgca
    24601 gagagttctt ctcgataaat cgacaaccag cgcaaggctt catgttcctt cggcaaataa
    24661 ctttgtatgc ggtcaaataa ctgattcggc tcttccagtt gctgaatttg tttatggaag
    24721 tagttaatac ttagtcgttt gtcatttact ttttcagcaa tttccggcaa atgatgcact
    24781 tcatcaatca acaaataagg actagctgga agcaatggtt cttttcgctg tgtttcttgt
    24841 actaaaaatg catggttgac tatcaaaaaa ttgctttgtt tgattttctt ttgcaagaaa
    24901 cgcacaaagt cttgtttgta aaatgactga ttccttgcta aaaacccagt gcctctgtgc
    24961 gcgacatctg caaagaatgc atggttcaga ttggttaaat gtaattcatc caaatcaccg
    25021 gtcttcgttt gtgttagcca aactaagaca gccatttggt acaaagcata ttgtttttgt
    25081 cccggcggtt ccacaagtgt acctttaaaa cgctgtaaat caatatagtg gcgatagctt
    25141 ttgataatca caggttgtat cggctgatct agcaattgat tcagtaatgg aatgtctttt
    25201 tccatgatct gctgctgcaa aacaagcgaa acggtgctga tgacagctgg tttttcaggt
    25261 gtagccaaat aactcaaagg caacagataa ccaatcgttt tgcccattcc tgtagctgct
    25321 tcgatcatca aatcttttga ttcatctttt gtgaaatggt cgtagacagc attcatcagc
    25381 ctgttctgtt cttttcgata catcagtgtt ttaccaaaca ttttttcttt tgcttgtttt
    25441 tttcttggat atgtccgctc gccataatgg acggatgtga atagctccac ttcttttttc
    25501 tgcaaagcta gcccatcgat gatttctaat gagagatcaa ggggctccgg gttttctttc
    25561 atttcttcaa gcacgtggta aatgaaacga ctcgtatcca ttcctgtatg gctacttaat
    25621 cgtgcaatct gctctaatgt gatgatcggc aatttcctca ttcgttcttc gatcagtaaa
    25681 agaagctgac ctgtcacttc ggcatcacta tccgcctgat gaggattctc atgaataaag
    25741 ccaaggcttt ctgacaaatc agctaagcga aaacttggtt ccgtcggcaa gaaaatttgt
    25801 gcaagttcta cggtatcgat gcccggtatc ttcaaactag gaagtccgca tcgcatgagt
    25861 tcatggttca aaaaattata atcaaaataa atattatgcg cgacaaatat ggtgtctgcc
    25921 aaaagattgt aaatcgtttg ggcgacatct tcaaaataag gcgctttttg gacgcgttga
    25981 ttcgtgatcc ctgttaaatg ctggatttgt tttgaaatct tccgccctgg atgaatatcg
    26041 atcgaaaaac gtgaagtgat ccgaccagct tcaatcatga cgcagccaaa ttggatgatc
    26101 cgatcttctt ttggattcgt cccagtcgtc tcgatatcaa cgactgcata agttcttggt
    26161 ttcaaaatat cttcctcttc tcctgcctct aatgttcttt tgaaattata ctacaaagtg
    26221 cagaaaaata ctctcatcgc tttttaagga aaaaaagaca aaaaagccca gacacataaa
    26281 tgtctggact tttattttta atataggtaa attaagcttt agtaacgttt gttgcttgag
    26341 gtccgcgttg accttcttca acgtcgaaag ttactgcttg gccttcttct aaagttttga
    26401 atccgtcgcc ttggatagct gagaaatgaa cgaatacatc gtttccgttt tctgcagtga
    26461 tgaatccaaa tcctttttct gagttaaacc attttactgt acctgtttcc ataataaaaa
    26521 tcctccttgt gtatcaacac atattttgca attacttgaa tggtgtatat ctggaagatg
    26581 tttatttgaa acaagctacc acagattacc gaacaaaaac tacattc
//
LOCUS       DAYYME010000026        26467 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000026 DAYYME010000000
VERSION     DAYYME010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.026"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..137)
                     /locus_tag="ACHBFW_000902"
     CDS             complement(<1..137)
                     /locus_tag="ACHBFW_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9317228.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     A"
     gene            complement(251..343)
                     /locus_tag="ACHBFW_000903"
                     /pseudo
     CDS             complement(251..343)
                     /locus_tag="ACHBFW_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(479..1114)
                     /locus_tag="ACHBFW_000904"
     CDS             complement(479..1114)
                     /locus_tag="ACHBFW_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9317229.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            1626..2768
                     /locus_tag="ACHBFW_000905"
     CDS             1626..2768
                     /locus_tag="ACHBFW_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM9317230.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            2761..2967
                     /locus_tag="ACHBFW_000906"
     CDS             2761..2967
                     /locus_tag="ACHBFW_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM9317231.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            3046..4239
                     /locus_tag="ACHBFW_000907"
     CDS             3046..4239
                     /locus_tag="ACHBFW_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9317232.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            4444..5583
                     /locus_tag="ACHBFW_000908"
     CDS             4444..5583
                     /locus_tag="ACHBFW_000908"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFM9317233.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5576..6376
                     /locus_tag="ACHBFW_000909"
     CDS             5576..6376
                     /locus_tag="ACHBFW_000909"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9317234.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6376..7203
                     /locus_tag="ACHBFW_000910"
     CDS             6376..7203
                     /locus_tag="ACHBFW_000910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9317235.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7208..7915
                     /locus_tag="ACHBFW_000911"
     CDS             7208..7915
                     /locus_tag="ACHBFW_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9317236.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            8015..8881
                     /gene="rfbA"
                     /locus_tag="ACHBFW_000912"
     CDS             8015..8881
                     /gene="rfbA"
                     /locus_tag="ACHBFW_000912"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFM9317237.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            8895..9467
                     /gene="rfbC"
                     /locus_tag="ACHBFW_000913"
     CDS             8895..9467
                     /gene="rfbC"
                     /locus_tag="ACHBFW_000913"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFM9317238.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9489..10517
                     /gene="rfbB"
                     /locus_tag="ACHBFW_000914"
     CDS             9489..10517
                     /gene="rfbB"
                     /locus_tag="ACHBFW_000914"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFM9317239.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10615..11466
                     /gene="rfbD"
                     /locus_tag="ACHBFW_000915"
     CDS             10615..11466
                     /gene="rfbD"
                     /locus_tag="ACHBFW_000915"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFM9317240.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11500..13533
                     /locus_tag="ACHBFW_000916"
     CDS             11500..13533
                     /locus_tag="ACHBFW_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317241.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13577..14857
                     /locus_tag="ACHBFW_000917"
     CDS             13577..14857
                     /locus_tag="ACHBFW_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFM9317242.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15067..15873
                     /locus_tag="ACHBFW_000918"
     CDS             15067..15873
                     /locus_tag="ACHBFW_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9317243.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            15885..17105
                     /locus_tag="ACHBFW_000919"
     CDS             15885..17105
                     /locus_tag="ACHBFW_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9317244.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17095..18681
                     /locus_tag="ACHBFW_000920"
     CDS             17095..18681
                     /locus_tag="ACHBFW_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9317245.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            18720..20858
                     /locus_tag="ACHBFW_000921"
     CDS             18720..20858
                     /locus_tag="ACHBFW_000921"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9317246.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21226..22209)
                     /locus_tag="ACHBFW_000922"
     CDS             complement(21226..22209)
                     /locus_tag="ACHBFW_000922"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM9317247.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22587..23978
                     /locus_tag="ACHBFW_000923"
     CDS             22587..23978
                     /locus_tag="ACHBFW_000923"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM9317248.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            23994..25034
                     /locus_tag="ACHBFW_000924"
     CDS             23994..25034
                     /locus_tag="ACHBFW_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9317249.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25053..26138
                     /locus_tag="ACHBFW_000925"
     CDS             25053..26138
                     /locus_tag="ACHBFW_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM9317250.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            26171..>26467
                     /locus_tag="ACHBFW_000926"
     CDS             26171..>26467
                     /locus_tag="ACHBFW_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFM9317251.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYP"
BASE COUNT         8595 a         4435 c         5392 g         8045 t
ORIGIN      
        1 atagcacctt tagaaagttt tgtttcattg ataatatctt gcaatgatgt gttatcatat
       61 cctttttcac aaaacatttt tgtagaaaca tctaaaatga gttttactgt ttcttctgga
      121 tatttatttc gtgccataac ttacctccaa tacaaaccgt tggtctgatt aaagtatatc
      181 ttagtatttt atgcttgtca atataaagac ttgcaatttt ataaaaatgc agaatgcaag
      241 cctttttcat ttattctaca atcttccagt tgctttcctt atgcggtgtc aattcatact
      301 gtgatacctg cgttgccttt gcctgattta ccacaaatca gattttatcc ctttcttcct
      361 cttgattgcc cacaacagaa aagaaaaagc cctgtcaaga gctttgccac cattggtggt
      421 gctttgcact cttgatcggg ctttttgttt gctgtatctt ctcgcaagag gaaaaaagtt
      481 actcgtcctc atcatcaaaa ttgttattcc ttaatacttc tcgtaacagt tccatatctt
      541 cttttgagtt cgggtttatc attttcacga cttgataagg tgtcttatac ttatgtccct
      601 ctatgctttc cccaaacata tccatactgt ataaatcatc atagcggtct aataattttt
      661 gaaacttcaa atgctggata aattctttga ttgggtaaag gtcggacgct tcaatgcttt
      721 tcccgttgat atagtctgta atatattccc gtaacttttc taactcatat tccagccatt
      781 cttcctcgct gtcaaagaag ttatcatagt gtccatgttt cagttctgca tttagacgtt
      841 cttcggctct taaaaactga cagacttcct tttcagattg attgacatac ttccattctc
      901 ccgacaacaa ttcattgggc gtaacgtcca gcacttccat tatcttttcc agcgtttcat
      961 aggcaggata gttcaaccct ctttcaatct tggaaaggct ctgcatattg ataccgattt
     1021 tgtccgcaag ttcctgttgt ttcattcccc taaattttct tatggtctgt atattatttc
     1081 ctaaatggct tattttcatt gcataaccct ccatatctcg ttatatcttc gctattataa
     1141 gtctatcatg cttaatatga tttgtaaagg caaaaaaaga tattgacaaa taagcatata
     1201 agaattacta tgttcttgta atcgaataag cacaatagaa tgaaatataa aaacattcaa
     1261 aaagaaatac ctatctgatt aaatccgtag atatgaatat cggttgaaat ggaacttgtt
     1321 agaaaaggca aaacagaact tgctgacaaa gtatttctga cacggtggcg gaagcctctc
     1381 aggcttaccg ccatgacacc ttgtgtcaag gggaagaaat gctttgtcgg ctggcgagcc
     1441 tgcgagcctt gtctgtgccc ccgttatcta acaggggggc actatttcac aaaagacaag
     1501 tcaaaaacca tacaccccca aacctgtgag tagtaatgag aggttttaag aagatagtac
     1561 atatcacact ttttgaaagg tggtttacag gtatttcaag gctattgtgg gattggagga
     1621 atacaatgaa caatttagaa tttgcacttg aaatgaagaa caaacgtctt gccagcggtc
     1681 tttcacaagg agaacttgca agtcttaccc atgtatcaag atatagtatc aaccgctttg
     1741 agaacggaaa agctaacgcc agcaaggaaa cacagaacat tatcctgcgt tgtctgaatt
     1801 actatatctg cgataagcct ttttatctgc tcgttgatta tctgtctgtc cgttttccaa
     1861 cgactgacgc attggaagtt atccgaaagg tgcttggcat gaaagcagat tattttatcc
     1921 attatgacta tgggtattat ggctataagg agcattatgc ttacggggaa atcaaggtca
     1981 tggcttctga tgatgaacac atgggcgtat ttttagaatt aaaaggggca ggctcacgca
     2041 acatggaata tgtcttacag gcacagaaca gggactggta ttcattctta aaccgctgtc
     2101 ttgactgtgg cggtgttatc cgacgttttg acttggcaat caatgatatg tgcggtctgc
     2161 tggatattcc tgtgctatct gaaaaataca gaaatggtgg tgcggaatgt cgctgtaaaa
     2221 actatgaaaa tgtacaaggt ggaaaattaa gcggaaaaaa ccgtaattta gcaagtaccc
     2281 tttatatcgg ctcaaaggca agcacaaaat atttctgcct gtatgaaaaa caaaaggaac
     2341 aggcaacgaa aaagaaacat accgatatta tcaaccgttt tgaaattcgt ctgcgtgaca
     2401 aaaaggcggt acaggcagtt gaagaactgt tattgacgta taaccctcat gggctggtgt
     2461 tttatctcat taccgatttt gtggaatttc ctgactatcc attgtgggag atattcattt
     2521 cccatgacag tttacctttt gaaatgaacc ctgtacctgt caatatggaa cgcactctgc
     2581 aatggctgga aagacaggtc atgccgtcaa tcgtgatgat agaggaaatc gacaggctga
     2641 caggttcaaa ctacatgaaa atgattgatg aatgtaccca cctttctgaa aaacaggaaa
     2701 tgcttgtgga acagatgtgt acggacatat cagacgtaat cgaaagtgag ggggtgtttt
     2761 atgagtaata cacaggatat tcccgtatgg gaaaaataca cccttaccat tgaagaagcg
     2821 tcaaagtatt tccgcattgg agaaaacaag ttaagacgat tggcagagga aaacaaggac
     2881 gctggctggc tcattatgaa tggcaaccgc atacagatta aacgccgaca gtttgaaaag
     2941 gttattgata aattggacgc aatctaatgc aaatgagcct tgtatgtgtt atgatgaaca
     3001 caagtcatat caaggctctt tccaacaagg aaaggagcag acaccatgaa agaaaaaaga
     3061 cgggataaca aaggtcgtat cctgcatact ggagagagcc aacgaacaga cggaaaatac
     3121 ttatataaat atgtagacgc attgggaaac acaaaatatg tgtatgcttg gagattgaca
     3181 cccacagacc cgacaccaaa gggaaaacgg gaaaaaccct cacttcgaga actggaacag
     3241 cagataagac gggatattga ggacggtatc gacagcacag gcaagaaaat gacgctttgc
     3301 caactctatg ccaaacagaa cgcacagagg acaaatgtga agaaaagcac acagaaacaa
     3361 cgggaacaac tcatgcggtt attaaaagag gacaagttag gtgctaggag cattgatacg
     3421 ataaaaccct ctgacgctaa agaatgggcg ttacgcatga aagacaaagg cttttcctac
     3481 aataccatta acaaccataa acgctcgtta aaagcgtcat tctacatagc catacaagat
     3541 gattatgtga ggaaaaaccc ttttgatttc aagctaagtg aagtcataga aaatgatacc
     3601 aaagagaaag tcgcattgac agaggaacag gagcaagcct tattgtcatt tattaagacg
     3661 gacaacgtgt atcacaagca ttatgatgat gtgctgatac tgttaaagac aggacttcgt
     3721 atctcggaac tgtgcggact gacagtagcc gacattgatt tcaagaatga agttgtgatt
     3781 atcgaccacc agttactaaa gagtaaggaa cagggctatt atattgaaac gcctaagacg
     3841 aaaagcggga taaggcaagt gccattaagc aaagaaacga tacaggcatt tcaacgggtt
     3901 atcaacagcc gaccaaagat aaagtcgata gaggtagacg gacacagcgg ttttgtgttt
     3961 atcaatccga aaggtaatcc aaaagttgcc attgattata acgccttatt tatccgtatg
     4021 gtaaagaaat acaacaagca ccacaaggac aatcccttgc cacatattac accgcatacg
     4081 ctacgccata cattctgcac aaggctggca agcaagaaca tgaacccgaa agatttacag
     4141 tatatcatgg ggcattcaaa catcagtatc acaatgaact ggtacgctca tgcgtccata
     4201 gataccgcaa aatcagaggt tcagcgtcta atcgcataga agtatttacc acgattttaa
     4261 ccacgattga cagggaaaat ataagaagat agacctagat atgcgaggtt taccacaaaa
     4321 gcaaaatgcc cgtagagccg ataaaataag gctttgcgga catttgagaa gatataaaaa
     4381 gatagtcaaa aagacatata taatttataa taattatgac tcttaattaa agtaggtgcc
     4441 catatgcttg tttcttttag tatggttata agattttttc tgacaatttt attatcactg
     4501 atattaacac ctgtattcaa aatcatttca gtacaaacag gtatggtcga caaaccaaat
     4561 gaaagaagaa taaataaagt tccaatgcct tcagcgggcg gactgcctat ctttatttca
     4621 tttgttatat cgactttatt cttattccgt aaaattatcc ctcaatcgta tattgtaccg
     4681 atcttgattg cttctttagt aatcatagtc acaggagtgc tcgatgataa atatgagctg
     4741 acacctaaac aaaaaagtat agggatcttg attgcttctc ttattattta ttttgtagca
     4801 gatatacgta ttgattcctt cacgcttcct tttattggat atatccaact aggctggctt
     4861 agttttcccg taacgatttt ttggatcttc ggtatcacga atgcggtcaa tctgattgac
     4921 ggtttggatg ggctggcggc agggatttca ttgatcggtt taattacgat aggaatcatt
     4981 ggatattttt tcctccatgc ttctacagtg tatattccag ttgttatttt ctgtcttgta
     5041 gcaagtatta tcggtttttt tccttacaat ttttatccgg caaagattta tttaggcgat
     5101 acaggagctc tattcctagg gtttatgatg gccgtccttt cactacaagg gctgaagaac
     5161 gtgacttttg tttcatcgat ttctttgctg atcgtcatgg gagtgccggt aacagacacg
     5221 ttttttgcta tcattaggag aaaagcaaac cgtgtttctt tttctacagc agataaaaaa
     5281 catcttcatc accgactaat cagtttaggc tttactcata aaggtgctgt cctgacgatt
     5341 tatgcgatgg ctttgatgtt ttctttcaca gctatggtca tgaattatac tggaagaatc
     5401 ggaacgatca tattgattat tgccatgttg tttgctgcaa ttttattatt tgaattgatt
     5461 ggtctgatca atgagaaaca tcagtttatt ttaaaaacat tgcgattttt agggaataaa
     5521 gagtaccgta cgcaagtaaa acagaaatat ttgaagaaat ggaaccgtca tatagatgaa
     5581 taacaaatta aaccagccga tccttattag tattgtgacg gcaaacagtg ataaaatttt
     5641 tcggacacta gataatttta tttcaactgt aaaagatgaa acagcagtaa aaatccgaat
     5701 ttttgacaac aattcacttc ccgagtacaa aaaaagacta acagagtata ggaaatatcc
     5761 ctacatcgat atctattttc atgatgaaaa ttccggattt ggttatggac ataatcacaa
     5821 tctgatagct agcgattctc gctatggcat tatttgtaac ccagatattt tagtggatga
     5881 agagacagtt acagcattgg tcttgttatt gaaagaacat ccggaatgtg cgatgttagc
     5941 acctaagatt cttaatgaag atggaacaac tcagcacctc atccgtgagc agctggctgt
     6001 ttttgattac gttttgcgct ttgtgccttt caaatgggtc aaaaaaaagt ttgacaaacg
     6061 actggctgtg tttgaatgcc gaggattgcc tactgatgct aatagttatg tacggatgat
     6121 ctcaggtagt tttatggtag ctgatattga taaatttaaa gagataaagg ggtttgatga
     6181 gcgtttcttc atgtactttg aagacaacga cttgtgtttg agttttgaaa aagcaggtta
     6241 caagttattg tacacaccct tttatgcagt tatccatttt tacgggaagg gtgcgcatcg
     6301 aaatgcaaaa ctgtttcgcg tgtttttgac ttctatgatg aaatttttca ataagtgggg
     6361 gtggcgtttt ttctgatgaa accagaaata ttagtagtcg ttgtgcttta tcagcaacta
     6421 ttcagtcaat ctccagctta tggaccatta agcaaagcac ttagcgaaaa gcaaattcag
     6481 cttattattt acgataatag tcctcaaagg cagcaaaatg agctgtttga aaaagaaaat
     6541 atcgtttatc atcatgatcc gcaaaatcct ggtttagcgg tggcttataa ttttgcgttg
     6601 tctcaagcag atgaaggaac acgttgtctc gtcactctgg atcaggatac gaggctagtc
     6661 gacgattatt ttgaactatt gcgtaaagta acctttacag atgaatgtgt tgctgcagtt
     6721 ccgatgattt tttctgggag ccgacaagtt tctcccctat attcagatcg ttatatcaat
     6781 cgtacagctc aagcagtaga agttgaaggc ccaacagctg aacgaatcat ggcaatcaat
     6841 tcaggtgttg cctggtctgt caaatttcta aaaaagattg gcgggtttaa tcctgctttt
     6901 tctttagatt ttttggatca ttggctgttt tggaaagtgg atcagttgga aaaaactgtg
     6961 gagatccttc ctgtacgaat ggaacatgat ctttcggttt tagactatga aaaagtttca
     7021 agtaatcgat accgttctat tttacaagca gaaaatcgat tttatcatga atatgatcaa
     7081 aggcatttat ccagtcaccg acgacaatta ttgttaagaa cagctaaaca gttcttgacc
     7141 gtcaaaaacc ggcatatctg gcggatgact ttgcgctcat atgttaagaa ttggaaggtg
     7201 tgaattcgtg cgttcggttt gtatagctac ctttaatgga ggcagatatt taagagagca
     7261 gttagatagt attttatctc agctttcagc agaagatgaa gtagtcattt ctgatgatgg
     7321 gtcagtagat gatacatggg atatattaac tgaatacgca gaaaaagata agcgaatcca
     7381 gcttttttcg gggcctcgtc aaggattgat cgctaatttt ggttatgcga tccagcagac
     7441 gaaaggagat ctaatctttt tggcagacca agatgatgtt tggttggaga caaaagtgaa
     7501 aaagatcact gactattttg agcagaatcc ggatcagcta gtagtagttt ctgatttaat
     7561 cattgtggac agcgagctgc aagagatcca tgattcgtat ttttcgtttc gaaattccaa
     7621 agaaggatgg cttaataatg tgataagaag tcactatatt ggtgctggta tggctttccg
     7681 gtcttcttta aaacccgata ttttgccgat cccgtcagaa gtgccgatgc atgatatgtg
     7741 gattggtttg ctgggaggaa agaagactgg gttcctaaga gaaccgttaa ctctgtaccg
     7801 aagacatgat ttaaacgcga gtgagattga aacacattct aaattacggc aaaaaatttc
     7861 ttggcgtgtt catctgatta cggcgttgat caaacgaaaa tggtttaaac gttgataaaa
     7921 gatgtttaaa tccttaatat ttccatgaca aatataacaa ttgatgagat aataacaaag
     7981 tgaaatttga aatcaaaaag gagagcagat atccatgaag ggaatcattc ttgctggggg
     8041 cagcggaaca cgtctgtacc ctttaacaaa ggcaacatcg aaacaattga tgccgattta
     8101 tgacaaacca atgatttatt atccaatgtc tactctgatg ttggcgggaa tcaaagaaat
     8161 tctgattatt tcaacgccac aagatacacc acgtttcaag gaattatttg gaaacggaaa
     8221 tgatttagga ctgcagatcg aatatgcagt acaagaaagt ccagatggat tggcacaagc
     8281 gtttatcatt ggagaagaat ttattggaga tgatagtgtc tgcttggtat taggggacaa
     8341 tatttactat ggtggcggtc tgtctaaaat gttgcaacga gcagcttcaa aagatacagg
     8401 cgcgacagta tttggctatc atgtcaatga tccagaacgc tttggtgtag tagaatttga
     8461 tgagaatatg caagctttgt cgattgaaga aaagcctgct cagccaaaat caaattacgc
     8521 tgtaactggc ctttattttt atgacaatga ggtaatttct atcgctaaag gtatcaaacc
     8581 ttcagaacgt ggagaactag agattacgga tgtcaataaa gcatatttgg ataaaggaaa
     8641 actatccgta gaacttatgg gacgcggatt tgcttggctt gatacaggaa cacatgaatc
     8701 attgcttgaa gcatctacct ttatcgaaac aattgaaaaa cgtcagaact taaaagtggc
     8761 ttgtttggaa gaaattgctt accgtatggg ctacatcgat aaggaacaat tgctggccct
     8821 tgcacagcca ttgaaaaaaa atcaatacgg tcaatattta ttgaatttgg ccgctgaata
     8881 aggagtcctt acagatgaaa gtaacagata caaaattaac agatgtcaaa atcgtagaaa
     8941 tggacgtctt tggcgatcat cgcggtttct tcacagaaag ttattcaaaa gcaaaatttg
     9001 cagaacatgg attgaaatat gactttgttc aagacaacca ttcactatca gctgaagctg
     9061 gtgtccttcg ggggcttcat ttccaaaaag gagaagctgc acagacaaaa ttgattcggg
     9121 tagtcactgg agcagtttta gatgtagtag tggatatccg caaaggtagc ccgacttatg
     9181 gtcaatggga cggatttatc ttatctgaac ataatcatcg acagttgctt gtacctaaag
     9241 gatatgccca tggattcgtt actttgacac caaatgtgaa cttcctttat aagtgtgata
     9301 attactataa tgcagaagca gatgggggca tcgcatttga tgatccagat ttagcgattg
     9361 attggccaat tgatccatca agagcgatta tgtcggaaaa agatcaccgt catccgacat
     9421 taaaagaatt tgaagcagaa aatccatttg tttacggaga aatctaagaa ttaaggagaa
     9481 attttcttat gaaaaacatc attgttaccg gaggtgccgg attcatcggc tctaatttcg
     9541 ttcattacgt ggtaaacaat catccagaag tgcatgtcac tgttttggat aaactgactt
     9601 atgcaggaaa caaagagaac ttggctggac ttcctagcga tcgcgtagaa ttggtcgtag
     9661 gagatgttgc agatgcagaa ttagtggacc gtctagtaaa agaagcggac gcagttgttc
     9721 attacgcggc agaatcccat aacgataatt cattaaaaga tcctttccca tttgtacaaa
     9781 cgaatatcat tggtacgtat acacttttag aagcttgccg caagtatgat gtacgctatc
     9841 atcacgtatc tactgacgag gtttacggcg atcttccatt acgtgaagac ctgccaggac
     9901 atggggaagg cgaaggagaa aaattcactg cggaaacacc atataatcct tcaagtccat
     9961 attcttctac aaaagctggt tcagatctgt tagtaaaagc atgggtgcgt tcttttggct
    10021 tacgtgcaac catttcaaac tgttcaaaca actatggtcc ttatcagcat attgaaaaat
    10081 ttattcctcg ccaagtaacg aatatcttaa gcggtattcg tccaaaatta tatggcgaag
    10141 gaaagaatgt tcgtgactgg atccatacga atgatcattc ttcagcagtg tggctgattt
    10201 tgacgaaagg cagaattggc gaaacctatc tgatcggtgc agacggtgaa gaagataata
    10261 agaccgtgat ggaattgatc ttagaaatga tgggtcaacc aaaagatgcg tacgaccatg
    10321 taaacgaccg tgcaggacac gacttgcgtt atgcaatcga ttcgacaaaa ttacgggaag
    10381 agttaggatg gaagcctgag tttacgaact tccgtgatgg gttggctgac acgatcaaat
    10441 ggtacgaaga acatgaagac tggtggaaaa aagaaaaaga ggccgttgaa gcagcatatg
    10501 ccaaaaatgg acaataatac agagaaaatg tcactgtatt aatggaaaaa cacatgtgag
    10561 gcaggcgctc gcctgtctct tttttaaaat aaaaatcaag gagcggataa tcatatgttt
    10621 ttattaactg gaggaaatgg gcaactaggt acagaattgc gccatttatt agatgaaaaa
    10681 aatctgcaat atgtttcaac agatgcaaaa gacttggata tcacagatgc cgagaaaaca
    10741 atggcttaca tcacagaatt aaaacctgat gttattttcc attgtgcagc ttacacagca
    10801 gtagataaag cagaagaaga agcaaaagaa ttagatgaga agatcaatgt tgatggtaca
    10861 agaaatgtag cacttgcagc aaaagctgtg aatgctcaat tcgtctatat cagtacagac
    10921 tatgttttcg atggaaaaaa caaaaatgta gaatacgaag tagacgacca aacgaatcca
    10981 ctaaatgaat atggtcgtac gaaattatta ggcgaaaaag cagtagaaga gattttagaa
    11041 gactattata tcatacgaac ttcttgggtt ttcgggatct atggacacaa tttcgtcttc
    11101 acgatgcaac gcctagcgga aacccgcgac aaactgactg tagtaaatga ccagtttggg
    11161 cgaccaactt ggacacgtac tttggcagaa tttatggtat atgtcatcga agaaaaagca
    11221 ccatttggta tttatcattt gtcgaacgaa aatagctgtt cttggtatga attcgcaaaa
    11281 gaaatattga aagatacaga tgtagaagtt gcacctgtta cgtcagaaga atttccgcaa
    11341 aaagcaacac gtccgcagta ttcagtcatg agcctgaaaa agacagaaga gttaggattt
    11401 gtcataccaa cttggcaaga agcacttgcg caaatgctgg aagctgccaa aaaaatgaat
    11461 aagtaatttc cttttatcaa atagaatttg gagcatgata tggaaaaaca aacaagaagt
    11521 gcacgacgaa aagaaaagcc aaaagcgaac caggcagatt cgaatttggt gcttgacctg
    11581 tttctaaaac tccgctatct tattggcgtt ttagtcatcg tggtcatcgt tacttttaat
    11641 ttaaacggaa gttcacttgg tgtttgggat aaatatgtgt cgcaaagaga cgatgggaaa
    11701 aaaactgatg tgatttttgg acagaatcgg gaagtgcggt cagacgaatg gctggtgcag
    11761 acaccttttt acctctcaca agctgaagag gactttccgt tggtgaacga ggattattca
    11821 ttaaacggac aaaacatgat cattgcgtat aattctcctg taaaggatat tacggttatc
    11881 ggaaaaccat tcaactgggg attctttttc ctcggaagag atcgaggact ttccttttat
    11941 tgggcgatga aattagtcgt catggtcttg cttggattcg aagtactgat gatattaacg
    12001 aagagaaaaa aagctctatc gctaatcggt gcatttgcgc tgacattttc tccagcagtc
    12061 cagtggtggt tcatgcaaca tgtgggcgat ttgattttct ttactttggg actgatgact
    12121 gctttttatc attatttcta caatcatgat aaaaaatggc tacgaacgtt gatgatgctt
    12181 ttggtggtta ttttcggtat tgggtttatt ttagtgattt acccagccca tcaagtgatg
    12241 ctggcgtact tgctgatttt ttattttgtt ggcctgctta tttactatag caagaaagtt
    12301 tcatgggatc tgttggatgt agtgttgatt ggaggggcag ttttatttat tggcggagtc
    12361 atggttcatt tctggctaac gtcaaaagac gcattgttag cttctttgaa taccttatat
    12421 ccaggaaaac gggtaagtac tggaggaaag tggacgattg gcgaattttt ttctttcctt
    12481 acaaattgga aaattccttt tgaagatatc aactattcga ataactctga agtggccctg
    12541 ttctatcact tttttccaac tgtattccta gcaagtccat ttgtgttatt tggcaaaaaa
    12601 gatagcgaac aaaagttact gggacgtatt ttgatgattt tttgtctgtt ttctatcttt
    12661 tggattaccg ttgggttgcc aaaatggcta gcagaaatca ctctgctgtc ttatgttcct
    12721 ccattaagag ccatccttac ttttagtttt gcggcttgtt tattatcgat ctggtttatc
    12781 gcttatattt ggagagaaga tgttattcct atttggtata aattcatttt gttggcagta
    12841 aatgcatggt cttatttcta tgcgattacg cattcgaaaa tggataatta tttttcggat
    12901 tttgaaacca tcgcagttgc tgtgttggga acagtgattt tagcttttgc attatttggc
    12961 tggagacgct tgttttacct cgcttttgcc gccttagtta tcgtatctgg ctttttcgtc
    13021 aatccagtcg tagaaggtac aggagcgatt tttgaaaaaa cactggcaaa agaaatccaa
    13081 aaaatcgatc aacaagatcc tggtaaagtt tggttgtctg aagatgaatt atataacttc
    13141 accccaacat tgggagtgaa atccttcaat acagtacgct tttatccaga tatagctgca
    13201 tggaagaaaa tcgatccgaa agaagaatac gagaagtatt ataatcggta tgctcataca
    13261 agagctttta tcgctaagga agatacaaag ttcattttag atcgtccaga tatgttttcg
    13321 gtcacgttga attttgaaga cgcagaaaaa ttagggatcg actatgtgat cagtaaacga
    13381 ccattaactg attacaacca attatatgaa gcgcagttca cgcagctata tggaccggat
    13441 aaagacggtt atatgatttt taaagtagaa tatccggatt atccagatgt accacaaaca
    13501 caaactcagg aaaatcagct agtcgtacag taataggcta gctgagaata atcctgaaat
    13561 gttagtagaa aggagagtgg agataattca agcaatcgta caaaaatttg tcaacaaaat
    13621 gagtgtcggt ttcttgcttt tgttactgac gctagtcttt tttgtttgga cagcagggtt
    13681 aggttttcca gcaactttgt ttatatataa caatagttca ttacttatcg cttttagtgt
    13741 ggctattttg ctcattttaa atgtttataa agcaaaaggc gtcgattttc tttatctagg
    13801 tgttgcgctt gcccttttca ttttcttttc ttactttcgg gaattaagaa atgagtcttt
    13861 atttggagac ttactgtttc ctttgattat tttattttgt ttagtaatta aatggatcga
    13921 gtggacaaaa tgggatcgcg gtatttatct gattgtcttt tcggtcttcc tcggaatcac
    13981 gttatatcgt gtatttacag agatccaagt accagaagga caaagcatct gggctccagg
    14041 caattcgctt tctgatattt ggatcaatac gaatataatc ggaagttctt tgatgactct
    14101 tgctttattg atcagtggat ttgccagttc ttttgaacga tggtatgtac gaatcatcgg
    14161 tgcagtagca gttgttgcag cttttctcgg gatttgggtc tgccaatcta gaggtgcatt
    14221 agtcgcgttg attgtttttg cgatattgga tcttttacct aaatcgttga tgcgtgtcat
    14281 tcgtgcgcca tttattgctt acacggttac gattctttta gctttgccga tttcttatct
    14341 ggcggctgtc tctgaaaaag tcaatttgtt taccggtcga gaagatattt ggcataaatt
    14401 ctatcagacg ctaggagaaa aatctgaaca aattttacta ggaatgaaaa catttatctt
    14461 ccaacgagga aaccaatttt taggaaacca caatagttat aattctattt tgaatatcta
    14521 tggattgata ggatttggga ttgctgcttt gctgcttatc ctttttattg gtcgcttaac
    14581 gttgaaagca gatttatcca acgggcagat gacttttatc tgggcctttt ttgcggtgat
    14641 gatgcagtca tttatggagg atacattgac ttcataccca tgggttccga ttatttatat
    14701 cctgttggct atggccgttc atcgatatga tgaaccagaa gttgcggagg aattctatgt
    14761 tatggaagag gcatatgacg atatgactta tgatgataca tcttatgagg aacaaccaga
    14821 tagcgctcat tcttccagga tccaacgcta tcattgatag gcacaaaata cgagagaggc
    14881 tgtgacaacc gtcgcagcct tttttggttt attgcaattg tctagcatct tctgtaaaat
    14941 agaaacgtta gagaaaatct tgtatcatag aaaattaaga aagaaataag aacgcagctc
    15001 ggtaccaaaa atatggtaaa atggcacttg actgtaattc actgaaaatc gcttggagga
    15061 acacaaatgt ttaaagattt gtatttattt atgaaggaca tttatcagaa cagaaaatta
    15121 ttgctacagt tttcgatcaa tgacttcaaa tcaagatatg caggatcgtt tttaggaatc
    15181 ttatgggcgt ttatcaatcc gctatttact gtattgattt attggctggt atttggtgtt
    15241 ggactgaaag cacaaatgat cgatgcaaaa tacccattta tcgtatattt agttaccggg
    15301 atgattccct ggttcttctt ctcagatgca tttgcatcaa ctacggttgt ttttcgggaa
    15361 tatacttatt tagtaaagaa agtcgtattc aatattcgta tcttgcccac gacgaaaatc
    15421 ctatccaatc tgtacaccca cttattcttc attctgattg gcatagctgt cgcgcttata
    15481 aatggtatat atccttcagc aatgtcttta cagctgattt attatttatt ttgcatgtgt
    15541 gctttcttga caggattgac ttggctgacg gcatcgatcc aaccattttt acctgatatc
    15601 atgcaattaa ttacgatcgt gctgcagttg atcatgtgga cattaccgat attatggagc
    15661 cctaatcagt ttgattcagg tcttgtaagc atattgaaat taaatccgct gtactatgtt
    15721 gttcaaggtt atcgtgagtc atttttaagt gaagcttggt tctgggagca tggaacatat
    15781 actttgtatt tctgggtttt cacgttagta atgttcgtaa tcggctctgc agtgttccgt
    15841 agattgaaac cacatttttc agatgtgctg taaggagaat agctatgaca gaatatgcaa
    15901 ttagactaaa agatatcaca aaatcttata atatgtacgc aaaaccttcg gatcgatttc
    15961 gtgaagccct caatcctttt aagaaatcct atcatgatgt attttatgcg ttgaaaaacg
    16021 tgaatgtaga aataaaaaaa ggtgagatga tcggtttcgt gggagaaaac ggatcgggaa
    16081 aatctacaat gttgaaaatc attacgggag tactgacacc aacatccggc acgatggaaa
    16141 tcgaaggaaa aatctcagct ttgctagaac taggatcagg attcaatcct gagtattctg
    16201 gctatgaaaa tatctattta aatggaatgg ttctaggatt ttcaagagaa gaagtcgatg
    16261 aaatggtcga tgatatcatt gaatttgctg acatcggaga ccatatttat caacctgtaa
    16321 aaacttattc aagcgggatg ttcgttcgtc tagcttttgc tgtagcaatc aatgtgaatc
    16381 ccgatatttt gattgtggat gaagcattgg cagtaggcga tcttgaattt caactaaaat
    16441 gtatggaaaa atttacagaa atcaagaact cgggaaagac gattttattc gtttctcatg
    16501 atatcagttc gattcgtcgt ttttgtgatc gttcctattg gttgaaaaac ggagaagtcg
    16561 tcgaatatgg cgatacgatg gaagtaacga cgaattatga aaacttttta aagaaaaaat
    16621 cagttaaaac agtggatcgg aataaaaacc aagtagaacg gttcgccgct ccagatatcg
    16681 tcgacgtgac gaaagcggaa ttgttggata aagacaaaaa tccggtggat atgctggaac
    16741 aaggagaaaa actatacgtt aaagtaactt atgaagtaaa agatgataca atcaaaaatc
    16801 cggttttagg aattgcgcta cgaacagtcg acaattttta tgtatgcgga cttaatactc
    16861 tactagatgg tatcaagatt ccttggaaaa aaggagagaa tatcttttat ctggagtatg
    16921 aaaagttagg tttgttagct ggtgaatatt attttgacgt tgcggtcttt gaagaaaatg
    16981 caacggttcc tttggtatat aagacaaagt acatgaactt gtttgtcagc ggttcttata
    17041 tcggagaagg catcgtggtc ttagaccata aatgggaaga agggactcat agcaatgaaa
    17101 tatgattttg aaatggacct agatgaacaa agcagtgtgg gaaaaattgc cgcacagatc
    17161 aaaccaggaa gtaaagtttt agagtttggt cccggaaacg gccgattaac gaaacattta
    17221 atcggagcaa aacaatgtga agtgagcatt gttgagttag ataaggaatt gttcgatttt
    17281 gtcagcgaat ttgctcaaga tggattttat ggtgatatcg aaagttttga gtgggcaaac
    17341 tattatgctg gacagacatt tgattacgtc ttgtttgcgg atgttttaga acatttggta
    17401 gatcctggaa aaacattgaa gaaagtcaga gaatttttga atgaagaagg agagatcctg
    17461 attacattcc ctaacttggc gcataattct gtcatgatcg atttgttcaa taaccaattg
    17521 ccgtgggctt cttatggtct cctagatgaa acccataatt ctttttatac tcatgatggc
    17581 ttccaaaaag tttttgaaaa agcgggatta ttcattaata ttgaagatta tttgtactta
    17641 gctgtaggtg atacagaatt aaaatcaact tatgaagaat tgccggaagc tgtacgctat
    17701 gatttcaaaa tgcgaccatt tggtgaagtc taccaatact ttttctcatt gatgaaacat
    17761 ccagtagcac aaagttccat tgcagagcca caaaattcta attatgtaaa agtgttagaa
    17821 gtaacccagc aaacgaaaca agatgaaaca atccaacaaa taccattcaa taatttcaca
    17881 ggggaaaatg agacactaag tttccccgta tcagaaacaa cagaaagaat ggtgtttcgc
    17941 ttcgcgcaac aacctagctt tatcgaattc agcgcagaag ctgccggaga aaaactaaca
    18001 tttatcgatt cgaatgctgt agtcaaaaca gtaaatgatt gttatttgtt tgatgggaaa
    18061 gaattaccag agttcgttct aacggatatt tctgggaaag aagtgactat ccattgtcac
    18121 tatcgcttta tcggggaatt gacaccaaca atgaaagaat tgttggaaac gattcgtccc
    18181 atggctgaag tcacaaagca gctttctgag aaaaatgacg aattagaaag agaaaatcat
    18241 catcttcttg aggaaaatac aagactagac catacgttaa aaacaaccac aaatcgctat
    18301 tgcacattgt tggaaagtga tgaatggaca ataaaacata gactaggacg acgtaagaaa
    18361 gaaacttcga aaaaaatcca agaaaaagaa ctttctatat gtattgacga gaaaatctgg
    18421 gatgcagaaa caaaaatatt aaaaatcatc ggatggggaa tcgctaattc ggatcgccag
    18481 cccttgtcct ataagttatc agctgaccaa tcgccatttt tcgaagcaat tcctgtttca
    18541 cgagaagaag tcaatcaagc agaacagctc gcaaaaggaa cagaagcggg atttgagtta
    18601 cgaatccttt gcgagcaaga acgttcattc ttagtggaag cagtagctca aaatggccaa
    18661 tcatggatca ttgaaatgta aataatgatt aacttataaa gataaggagt aaacgaatcg
    18721 tgcaaaaaaa agaaattgcc gtatttatcg atcaaatcac tcgtgaacgg gccactggcg
    18781 atttgcttat tgtgggctgg gcaattgatg aagtgaccaa agaaatccct acgataaagg
    18841 tagaaaaaga aaatgtgatt gctgaagcaa cacatgtcgt aagattggat atcaatcatt
    18901 tatataatct tgatgttaag acccaaagcg gttttaaggt acgtttatca gggaaaatga
    18961 gaggaaaagc catcttagac ttccaaacag cgagacatca aaatggcata gctgtgaaat
    19021 taaacggtaa atacccttat gatgacggca ttgaatcttc atgggaaaga aaaaaaagat
    19081 tactgaaaaa agggataaat tatgcaagga ctcatggcgt aaaaaaagcg atccgtcggg
    19141 tcaaattgga attgaagcca ggatcgatcg actatgcaga gtggatcagt cgtcatgaga
    19201 aaccagattt gaaagagcaa aggaaagaag cacaaggatt tgcgtaccgt ccattgattt
    19261 ccatacttat gcctgtctat aacgttgaaa tcaaatggct cgaaaaatgc attgattcgg
    19321 tactggatca aacgtatgat cattgggaac tatgtatcag tgatgatgct tctactgatc
    19381 cagctatccg aaagtgtctg gaatcctatc aagcaaaaga tgatagaatc aaagtcgttt
    19441 tccgaaaaga aaatggacac atcagtctgg caactaattc tgcattggaa atagcagaag
    19501 gagaatttat agcactctta gataatgatg atgaattacc gccatttgca ttatatgaag
    19561 tggcaaaagt gctaaacgta catccagaac tggatgtgat ctacagtgac gaagataaaa
    19621 tcgatgcaga tggcaatcgc tttgatccgc atttcaaagc agactggtcg cctgatacat
    19681 tgatgggaaa taattatatc tctcacttag gcgtatatcg aacaagcatt gttaaagaac
    19741 ttggcggttt ccgtaaaggc tatgaaggtt ctcaagatta tgatttggtc cttcgtgtga
    19801 cagaacaaat ccctgcagat catatttacc acattgaccg agtcttgtat cattggcgga
    19861 cgatcccagg atcaactgca agtaatggcg aagccaaaag ttatatctat gattccggag
    19921 tgaaagcatt gacagatgcc ctgtctagaa gaaacatcaa aggaagcgta cgtcctggca
    19981 gaatttctgg attttatgag attgcttatg acgtcttgca agaggatttg gtcagtgtga
    20041 tcattccaac gaagaatggc tatgaagatc taaaaacttg tgtggattcg atcatcgaaa
    20101 aaacgtctta ttcaaattat gaaatcatta ttgcggataa cggaagtaca gatccaaaaa
    20161 tgcaagaatt atttgcagaa tacaagcatc agctgaaaga ccgctttatc gtagaattga
    20221 ttgacattcc attcaattat tctcgaatta acaatttagc agcagaaaaa gcaaacggca
    20281 aatacttttt attcttgaat aacgatacag aagtgattga gccagattgg atgacggtga
    20341 tggtttctta tgcacagttt gatcgaatcg gctgtgtagg agcaaaactg ttttatccag
    20401 atgatacaac gcagcatgca ggagtattat taggaatcgg tggtgttgca gggcatgcat
    20461 tgaataacta tgatcgaaca cattgtggtt atttcggccg attggtcatt gacgtcaatt
    20521 acttagcagt cactgcagct tgtatgatgg tcaaggcagc tgacttcaat gctgttaacg
    20581 ggtttgatga aacattagaa gtagcattca acgatgttga cttgtgcttg aaagtttatg
    20641 aactgggtcg ttacaatgtc tatgcacatc aagcagaatt gtaccatttc gaatctaaat
    20701 caagaggtta tgaagatact ccagaaaaac aaagacgttt tgctggtgaa atcaaaaaaa
    20761 tgcaagataa atggccagca tatattgcgc atgatccatt ttacaatgat aatttaacaa
    20821 aagaaggaat cggcgacttt tcgttgagac cagattaaac tataataaaa agctgttatg
    20881 ttgtgattcg ctcgcacata acggcttttt atagtttttt gtcaaaaaaa ggcagaaggt
    20941 gccgactcaa aaaaataagt cgaggttggg acagaagtgt ttaactccga gaaataagaa
    21001 gaaattcacg aaaattgctt ttcaaatttt tgtgaatttc ggcttattta agaagaagtt
    21061 gcttctgctc ccaccgttta ttcgttttta aagaatgaga caaaagtgtt tcttactttt
    21121 gtcccacgct cttattcatg ctcagagcgc caatagatac aaaatcatag ccaaagtttc
    21181 acgaacccta gctgtcaaag aatcgatatt ttattttaat ataggctatt ttaaaataaa
    21241 tggtttaagg tattcctcta caaaagccac tctatcaatt atcatgggtt taaaataaga
    21301 agtgatggag ataaccagat tgttcttagc gtcaatataa agaacatttc cactattacc
    21361 aattgctgaa tatatttcgt tctcagaatc taatacccac cataaatagc catatctcat
    21421 attttgtaag tcttctaaag cactggttct aggtttgaac atctcgttta tccacatggg
    21481 tggtacgatt tgttttcctt tatacatccc tttatttagg cataatacac caatttttgc
    21541 cagatcactt gccgataaac acaaaccata tcctgccgtc ccgatattta gtggatcaca
    21601 aaaccagaca ttggttttag gagacttatc cattgtaaac tgcttatgtt cttcagctgt
    21661 ttcagcataa taattgacat ggtttggtat ccctatagga tcaaatagat acttattggc
    21721 aaaatcaaca gttgttagct tacttgtctg atacaacaag ccagttaata tgtgtaaaca
    21781 gacagtttga tagttgaatt cgccggttat accttttctt cctcccaata aatctagtga
    21841 tgtttttgtc caatcttcac ttgaacagac ttttgtccat ggatcgcctt tacatttgta
    21901 gggtgctttc attgttagca agtgcttgat tgtaatagcg taaatagttt tctccccgcg
    21961 tttcacttta tactctggaa aataatctaa tactttatca tcgatacttt tgattaatcc
    22021 ttgctctaaa gcaatgccaa tcagcaaaga tacaatactt ttcgttgctg acatgatatg
    22081 acaagtatcg tcttttttat aaccattcca ttcatctgta taaacttcta ccccatcctt
    22141 atatgcgaca atttgacata tatttggttg tcgaaaacca ataaaatcat gcaactcttg
    22201 tttattcata agatattacc tcactttctt ctataagcat aatgtcatta aaaatcgatt
    22261 tcaatttttt cgattcttca cgtaatacag gtattcttat ggactagaca gcgctttggg
    22321 aagatggatt ctctgaaaaa tataaaggtt cactttatga gtggataaaa aaagcagcaa
    22381 atacttcgac ttctttaatt taccaacatt agtgaaaatc gattcatctg gagatttgta
    22441 atttagagtc cttgtcttga ttagaggtgt ataaagcttt ttattagaca tttttaaatt
    22501 ccattacaat ttgcttataa actaggtttt taaaggtgac tatggtaaga taatagagat
    22561 attattttgt gattaggagt gtatatatga ataaaaatgg agaatggagt gctgcacgac
    22621 ggatattcat tatattaatg gatgttattg tatacaatgc agcaatatat agtagtttct
    22681 tactgaagtt tcgtggagag atcccattac gaaatttaga aactttccaa cactcagcaa
    22741 ttattatttc agtgattttt attgcgttga acattttgtt gggtgcatat gttttttata
    22801 atcgaatgat cagtgacatc gtgtttgtca cagtcatcgg tcaggtattg atgacactag
    22861 ggatcatggt agtgactttt gcgggccgct ggtttgcttt tccacgtctt gttatcttac
    22921 tttcatttgt tctcggtacg cttttcttaa gtatctatcg tattctcgta tataaacttt
    22981 atttacgcgt aagtcgagat aaaaaggtcg tcattattgg gttagaaaag gatgtcgcac
    23041 cagcaatcca aaattttggt tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc
    23101 ttgataacta ttatgcaaac atcaaaaata tgattaaaga catcgatatt gtctatcttg
    23161 cttctcatat cgaagaaact gaaaaaatca aaatctatca attagttact aaaaatgaaa
    23221 aaaaattgtt tttgaataca acatttgaaa acttgacatt gatcaatcca aatattatga
    23281 atttcgaaga tgaaagtatt attgaagcat ctggatttag aatccaacct gaatatgaac
    23341 tattcaaacg tttgtttgat atcatcgttt cacttgtttt attagttatt gcttcaccat
    23401 ttatgttatt cacagcaatt ttagtcaaag tgacatcacc gggaccaatc atctataaac
    23461 aagtgcggat cacaaaaaat caaaaggaat tttcaatcta taaattccgc tcgatgaccg
    23521 ccacagccga agcaaaatct ggaccagttt tagctaagag caatgatgca cgagtaacgc
    23581 ctgtaggtaa atttattcga gctgtccgct ttgatgaatt accacaaatc ttcaatgtgc
    23641 tgaaaggcga tatgtcgatc gtaggaccac gtcctgaacg tcctttcttc gtcgatcagt
    23701 tcaatgcaga aaacccttat tactacttac ggcataatgt tcgtgctggt atcactgggt
    23761 atgcacaagt ctatggaaaa tatgtctctg attacaacag taaattacgt tttgatttac
    23821 tttatatcaa aaaatattca ttgatgcttg atttaaagat cttgctgcaa accatcaaga
    23881 tcctatttga taaaatgtcc tctcaaggac ttgaagaaga cgaaaatgtg atctcattgg
    23941 cagaaattgc ttttccgaat gaaaaaatct ttcgctagtt gggaggcgta aaaatgaaca
    24001 atccgaaatt cagcataatc gtaccagtat acaatgcaga agcaactatt ggtcgaacga
    24061 ttgatattct aaaagaattg gattatgaag agtacgaagt cgttttagta aatgatggat
    24121 caacagatca aacacaggaa atcattcaag aactgattga aaatgatgaa cgttttgaat
    24181 tgattactac gaccaatcaa gggcccggat tagcaagaaa cgaaggaata agacagtcaa
    24241 aaggagaata tctcttattc ttcgatgcag atgacacgcc agtaaaaacg atcttgtcag
    24301 attatgatca tttgctagaa aagagtccaa aagcggatct tgttgttagt tcgtttgttt
    24361 ttcgtacaat ggacgaggga agaatcgttt ccgaaaaaga aaacttagtt acagaatgtc
    24421 actatctaac tcacgaagag tttataaaag acatgtatca gttaatgaat gaacagttga
    24481 tgtatgttgt atggaacaaa tgctatcgta gcgatattct gaaaaataac caaattctat
    24541 ttaaaactta taatagttgt gaggatcgaa tatttaactt acattattat gaatactgtc
    24601 atcaagtgat catgaaccca aaagtagaat atatttatga atttgaaggt gggaagggga
    24661 tcactaatca ataccgacct aacaagttcg aaacatttaa agaattctat tcattagcaa
    24721 atactgtaac agacgatatt aataaagacg gtatggcggc gcttctttta aaagggacaa
    24781 cttcagttat tttttctatt tatagtacct ctgcaagaac ttcaaaagag aagaaagacg
    24841 aagcaaagca gatattgagt gatccagtga tccgagaagc caagcaaatt gctaagacgg
    24901 attcttcagc aaaaaaaata acaaagttgc tttacaatat gccagctcca ctatttttat
    24961 ctgctgtaaa agttggaagc ttcgttgaag tgaaaatgcc agggttaatg gctgctttga
    25021 agcgagtata ttagttaatg gaggaaaata taatgaaaag aattttgcat tttcagggac
    25081 gaatgggttt aggtggagca gagtctttca tgatgaattt atatcgtaaa attgatcgta
    25141 caaactatca atttgatttt ttgatttatg aagattatgc agatgttcaa gactatcatt
    25201 cagagattga aagattagga ggccgtattt ttgttgtacc taatcctaag aaaaatattt
    25261 ttaaatattt aatagaagtg aataagttac taaaaaaaga atctttttct atagttcaca
    25321 atcaggttta ttttggcggg ggaattaatt tatggttagc caaaaagaat ggaatcagac
    25381 agagaattgc tcatagccat gcaactgaag atggaaaatc tcaaaatatt gtaatgaatg
    25441 tattgcgtaa atttttaacc aaattgttgt tacaaaatgc aacagactac cttgccgtta
    25501 gtcaagaagc tggtgaaagt ctgtttcaaa accacccctt tgagatagtt cataatggta
    25561 ttgatttaga gctttatagt aaaaattcag aagctaaggt taataagaga aaagaattgg
    25621 atatctcaat gagtactttt gttgtgggaa acattggacg attagagaaa caaaaaaatc
    25681 aatcttattt gttagagatt tttttagaaa tactaaaaat gaattctgat tcttacttga
    25741 ttttagtagg tgaagggtca cttagaaata aactgaaagc caaagcagta gaattggata
    25801 tagagaaaag agtaaaattt ttaggagaaa gaaacgatat tcccgagtta ttatctgtga
    25861 tggatgtttt tgtaatgcct tctctttatg aaggtctgcc aatggttgga ttagaagcac
    25921 aagcagcaaa attaaaatta gtcttagcag atactatttc caaagataca aagttaacaa
    25981 ataatgtgga ttttgtatca ttagatgaaa caccacaaga atgggcaaaa aaagtaataa
    26041 aagagccgta tgctaataaa ataacacaag aactaaagag ttatgatgtt tcgtacacta
    26101 ctgaacaaat ggagagtata tatagtagag atcattaaaa atgttcaaga tattttagga
    26161 gtgattattt ttgaaaatat gttatttgat attggcatac aatgatattg gcaacttaaa
    26221 gagattggtc aatcgattga acgaaaatgc agtttttatt attcatatag ataaaaaaac
    26281 tgatatagaa ccatttattg aagcatttaa atcatatgat aatgttgaat ttttatgtaa
    26341 taaaaaccgt gttaaaattt attggggtgg tttttccatt gtacaagcag aaatcaattt
    26401 agtaaagaga gcattacaaa atgagaaata tttaaaatat gtattattat ctggtgctga
    26461 ttatcca
//
LOCUS       DAYYME010000027        25437 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000027 DAYYME010000000
VERSION     DAYYME010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.027"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..400
                     /locus_tag="ACHBFW_000927"
     CDS             <1..400
                     /locus_tag="ACHBFW_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HFM9317252.1"
                     /translation="KPMETGEGLTLNERKIYQYIHPNVCESCQLQMGYTILEPGSAWN
                     TMPCHTHERRMEAYVYFDYANEDTRVFHMMGKPDETKHLVVDNEQAIISPSWSIHSGV
                     GTSNYSFIWAMCGENITYTDMDMVQMDQLK"
     gene            420..1223
                     /locus_tag="ACHBFW_000928"
     CDS             420..1223
                     /locus_tag="ACHBFW_000928"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFM9317253.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(1472..2536)
                     /gene="fni"
                     /locus_tag="ACHBFW_000929"
     CDS             complement(1472..2536)
                     /gene="fni"
                     /locus_tag="ACHBFW_000929"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFM9317254.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(2533..3618)
                     /locus_tag="ACHBFW_000930"
     CDS             complement(2533..3618)
                     /locus_tag="ACHBFW_000930"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFM9317255.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(3631..4608)
                     /gene="mvaD"
                     /locus_tag="ACHBFW_000931"
     CDS             complement(3631..4608)
                     /gene="mvaD"
                     /locus_tag="ACHBFW_000931"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFM9317256.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(4601..5545)
                     /gene="mvk"
                     /locus_tag="ACHBFW_000932"
     CDS             complement(4601..5545)
                     /gene="mvk"
                     /locus_tag="ACHBFW_000932"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFM9317257.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5861..6520
                     /locus_tag="ACHBFW_000933"
     CDS             5861..6520
                     /locus_tag="ACHBFW_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM9317258.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            6605..7510
                     /locus_tag="ACHBFW_000934"
     CDS             6605..7510
                     /locus_tag="ACHBFW_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM9317259.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(7512..8144)
                     /locus_tag="ACHBFW_000935"
     CDS             complement(7512..8144)
                     /locus_tag="ACHBFW_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM9317260.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    8295..8420
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            8464..8988
                     /gene="infC"
                     /locus_tag="ACHBFW_000936"
     CDS             8464..8988
                     /gene="infC"
                     /locus_tag="ACHBFW_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFM9317261.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            9060..9260
                     /gene="rpmI"
                     /locus_tag="ACHBFW_000937"
     CDS             9060..9260
                     /gene="rpmI"
                     /locus_tag="ACHBFW_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFM9317262.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            9313..9672
                     /gene="rplT"
                     /locus_tag="ACHBFW_000938"
     CDS             9313..9672
                     /gene="rplT"
                     /locus_tag="ACHBFW_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFM9317263.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            9924..11261
                     /locus_tag="ACHBFW_000939"
     CDS             9924..11261
                     /locus_tag="ACHBFW_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFM9317264.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            11309..13438
                     /locus_tag="ACHBFW_000940"
     CDS             11309..13438
                     /locus_tag="ACHBFW_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFM9317265.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            13413..14102
                     /locus_tag="ACHBFW_000941"
     CDS             13413..14102
                     /locus_tag="ACHBFW_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317266.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(14510..15187)
                     /locus_tag="ACHBFW_000942"
     CDS             complement(14510..15187)
                     /locus_tag="ACHBFW_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9317267.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(15323..15517)
                     /locus_tag="ACHBFW_000943"
     CDS             complement(15323..15517)
                     /locus_tag="ACHBFW_000943"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317268.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(15567..16007)
                     /locus_tag="ACHBFW_000944"
     CDS             complement(15567..16007)
                     /locus_tag="ACHBFW_000944"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFM9317269.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(16011..16856)
                     /locus_tag="ACHBFW_000945"
     CDS             complement(16011..16856)
                     /locus_tag="ACHBFW_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFM9317270.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            17005..18423
                     /gene="pepV"
                     /locus_tag="ACHBFW_000946"
     CDS             17005..18423
                     /gene="pepV"
                     /locus_tag="ACHBFW_000946"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFM9317271.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(18480..19427)
                     /locus_tag="ACHBFW_000947"
     CDS             complement(18480..19427)
                     /locus_tag="ACHBFW_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9317272.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(19650..20000)
                     /locus_tag="ACHBFW_000948"
     CDS             complement(19650..20000)
                     /locus_tag="ACHBFW_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM9317273.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            20200..21279
                     /gene="pepA"
                     /locus_tag="ACHBFW_000949"
     CDS             20200..21279
                     /gene="pepA"
                     /locus_tag="ACHBFW_000949"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFM9317274.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            21423..21743
                     /locus_tag="ACHBFW_000950"
     CDS             21423..21743
                     /locus_tag="ACHBFW_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM9317275.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            21765..22229
                     /locus_tag="ACHBFW_000951"
     CDS             21765..22229
                     /locus_tag="ACHBFW_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM9317276.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            22434..23039
                     /gene="ytpR"
                     /locus_tag="ACHBFW_000952"
     CDS             22434..23039
                     /gene="ytpR"
                     /locus_tag="ACHBFW_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFM9317277.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(23078..24367)
                     /locus_tag="ACHBFW_000953"
     CDS             complement(23078..24367)
                     /locus_tag="ACHBFW_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFM9317278.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            24795..25274
                     /gene="rlmH"
                     /locus_tag="ACHBFW_000954"
     CDS             24795..25274
                     /gene="rlmH"
                     /locus_tag="ACHBFW_000954"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFM9317279.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT         7880 a         4816 c         5251 g         7490 t
ORIGIN      
        1 aaaaccaatg gagactggtg aaggcctgac actgaatgaa cgtaaaatct accaatacat
       61 ccatccaaat gtatgcgaaa gctgtcaatt acaaatgggt tatacgattt tagaaccagg
      121 aagcgcttgg aatacgatgc cttgtcatac acatgaacgt cgtatggaag cttatgtcta
      181 ctttgattat gccaatgaag atacacgtgt gttccacatg atgggcaaac cagatgaaac
      241 aaaacattta gttgtagaca atgaacaagc cattatttcg ccaagctggt cgatccattc
      301 aggtgtaggt acaagcaatt actcattcat ctgggcaatg tgcggagaaa atatcaccta
      361 tacagacatg gatatggtgc aaatggatca attaaaataa gcataaggga gagaaaatga
      421 tggaatttag tatggatatg tttcgtttag atggaaaagt agcattagtc accggtgctg
      481 tctatggtat tggatttgag attgcacgct ctttagctac agcaggagca aaaatcgtat
      541 ttaataactt gaatcaagaa tcagttgatg aaggtatagc gcattataaa gaagcaggca
      601 tcgatgcaaa aggttatgtc tgcgatgtga ccgatgaaga agcggttcaa gcgatggttc
      661 aacagatcaa agaagatgta ggttcagttg atattctagt caacaatgca gggatcatca
      721 aacggacacc gatgatcgaa atggatgcgg cagacttccg ccaagtaatc gacgtggatt
      781 taaatgcccc attcatcgtt tcaaaagcag ttattccgga tatgatcgaa aaaggcggcg
      841 gtaaaatcat caatatctgc tccatgatga gtgaattagg acgcgaaacg gtcagtgctt
      901 atgctgcggc aaaaggcgga ttaaaaatgc tgacgaaaaa catcgcttct gaatacggtc
      961 aatacaatat ccaatgtaat ggaattggac caggttatat tgctacaccg caaactgctc
     1021 cattacgcga aacacaagaa aacggtgaac gtcatccatt cgatcaattt atcgttggac
     1081 gtacgccagc agcacgctgg ggcgagccaa tcgatttggc tggaccatct gtcttcttgg
     1141 cttctagcgc atctgatttc gttaacggcc atgtcttgta tgtggacgga ggcatacttg
     1201 cctatatcgg aaaacaacct taaaagagct ttagtacact gtttagtgga gaggttgtga
     1261 aatcagcgga ttgaacgaat accgaaggac agctgattaa acatcattta ttcgaaaaaa
     1321 gaggctggga cagaagcatt taactccaag aaataagaag gaattcacga aaattgcttt
     1381 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttttacttc cgccgtttgt
     1441 acgtgtttag agcgtgagac aaaagtgttc tttacttttg tcccacgctc ttttctgtgc
     1501 gtcttccata cttcactaag tcgatccctc ttgcttcgca ccaatccttc actggtccgg
     1561 aaaagatcag agattgttgg tgtagggccg cggtattcgt tgcgccgacc atagtataaa
     1621 gcagacgaag ctcttcttgc cattgcttca tcagtataat agtttcttct acgccgtgat
     1681 tcatcagatg cgtcagtacg gttcctgaag tacccacggc atttgcgccg agacaaaggg
     1741 ctttaaatat gtcataggca ttgcggatac ctccggaggc taggatttcc atagaagtgt
     1801 ctgcttcatt tgcttcaagc aatgaagaga ctgtcgattg cccccagtca gctaaataac
     1861 tcagttcccg cttggagcga cgggcgtttt cgatctgggt gaagcttgtt ccgcttcgac
     1921 cgctgatatc aactgtatga actcctaaag cagctagatc attgagcgtt tctcttgtca
     1981 tgccaaaacc gacttctttt acgatgaggg ggacgtcaat ggctgtttga gtttcttgaa
     2041 taagtacttt ccaattggta aaatcacggt ctccctcagg cataaccaac tcttgaggtg
     2101 cattcaaatg aatctgtaag gcatctgcgt gaaacaatcg gatagcttct ttggctcttt
     2161 cgacagaagt ccctgcaccg atgttggcaa tgattttccc gtgaggatat tcttgtcgca
     2221 taatcgtata agtatcggct aaagaaggga ctttcaaggc agcgctgact gaaccagtgg
     2281 caatcatcaa atctgcttct cttgcgacga ttgcaagatc ttggttgatt ttttttgttt
     2341 tttcgcttcc gccagtcatc gcattgatat aaaatgggct gcttagtgta aacccaactg
     2401 cctgtgtact tagatcgaca tcagaaactg ctatttgcgg caaagggtta tggatgatcc
     2461 gtacgaagtc aaattcgttt ttttgtttat catgaaaggc tttagctagt gaaacatgtt
     2521 cgtcttttcg attcatcggt tttcctttct ttgatcgcta tagacatgta acggcagtgg
     2581 agtgatttct gctttttccc atgcactcat taaaggaaga atgccagatt tctggtcaac
     2641 aattacgatt ccgcaatcgc ccccacctgc accagaagat tttgcggctc cttcatactg
     2701 ttcagctaaa ttacacaatt tgttcaaagc aggcgtttcg atgacgaccc cagtgattgc
     2761 agataaatca tgcagtaatt gtcggttttt tcgaatcatc tgttggatca acgttacatt
     2821 attttcttta aaccctttga tcatttcgtt gacacattct gtactatttt ttaagaaaag
     2881 ctgataagcc accattttat cttctctcga acggtgaact tgatcgacca aatcagaagt
     2941 agaggcaggg ctacccgtcc aaccaatcaa taaacgtaaa tcttcaggag caatcaatgg
     3001 ctcaatggat agacctggcc aatccagggc aagtaactca ctgatagaat gctgagtttc
     3061 ttgttcttgg agccaaggat ggtcgaaggt tgagaaagcg atccagccac cataacagct
     3121 agcagcgatg tcgccgcagg aaccattatc ttgaactgct aaattggcta gtgctgcaat
     3181 cttgaaaatc tccaactgag ataaattcaa cgcgtaaaaa acattcaagg ctttgaccgt
     3241 tgcaacggtc acggcaccgc tagaacccaa tccatatttc cggccatttg aactgtctaa
     3301 ctcacttgtt actttcagat cataaaagga taaaagaatg tttttttctt gtgcatactt
     3361 ttcagtcaag cgaatagcag caaggatata atgaaaagga ttttcccgaa tatctaaaac
     3421 taattctccg ttgcgtcttg tccaacgtac aggcatccca ctatattgtg cagattggat
     3481 acttccgact tttcgtgcag attctacagt gactgtcacg aattgatcga ctgcagcgat
     3541 aactgctgga tggcctgttt caacaactgc atattctccg gcaatataga gtttgcctgg
     3601 tgcagatact tcaatcataa aaatctccct ttattcaata atcgcaattc ctggaccagc
     3661 atgtgcagta attacttgtt ctttgctaaa taatttggta aatgtttctt gtacctcaga
     3721 taaatggctg ttttccacta aaactttgac attagggcca gcatccatcg taaagtagca
     3781 tggaatgcct tgctttctca attgtcgcac cgcatccatc gcttttaagc tgtcaggaga
     3841 ccagtaagtg aatggaggct gggcagctaa agtcgttcca tgcattttta gtccgtttct
     3901 ttccatcgtt tctccgagaa gttggaaatc ttttgttttg attgcttgtt tcaattgata
     3961 tagatcccct tcaacggaat ctaaccagcc ttgataaaaa ctggatgttt cgactgtccg
     4021 acgcatccca tttctgctgg acacttcttt tttctggtcg ttgatcaaaa cgaaaaccat
     4081 tgctaaatcg tcttcgaaag aatcggaagg gactggctta gcgtaagaac ttaagtcgtc
     4141 atggcctttt tcccattcga cgaaaccacc gaaaatactt cggcaagctg aaccagaccc
     4201 gcgtcgagca aatcttgaaa gagagagatc atctaatcca agcttaagag cagtattgca
     4261 agcgcctgct aaagcagcta gcccgctggc agaggaggct aatccagctg cggtaggaac
     4321 gaaattctga ctgattactg aagcttttag tgagaggccg gcttctttac gaaaaaggtc
     4381 aagaaactga ctgacttttt ttgttgcttt ttcgtcttgc aaggtgccat ctagataaaa
     4441 ttcatctacc atatagctat cagaaaagat gacttctgtc tctgtataaa atgcatccaa
     4501 tgttaacgaa aggctgttgt tcattggaag gatgagttct tcatttttct taccccaata
     4561 tttgattaga gcaatgttgg tatatgcgcg tgctttgcct ttaaacatag gtatgtactc
     4621 ctaatccttg tatccaagtt tcagcagcac ccgcatcttc aagtgcttga ctgatttctt
     4681 gggcttttgt ttttgtttgt gctaatgcaa tcatacagcc accgcgtcca ccgccagtta
     4741 atttggcacc caatgccccg gtatcttttg ctgtttggat caatagatta agaaaatcat
     4801 tgctgattgt cagcttttcc agaagtgatt gtgattcatc cattgtctgt gccaacgttt
     4861 ctggtgaatt ttcaatgatt gcttgttttg cttgcttcgt caagtatcct aatttttgga
     4921 tcatttgtcc agtttcatga ggctgggttt caaagaggtg agcgacatct ttgacggctt
     4981 ctcttgtttg tcctttgatt cctgtatcag cgacaatcaa aaaagcatcg atgttcaaag
     5041 aaaagtagtc gaaaggatgc ccttttgtaa aataaatcgg ctgcaagctg ctagtagcgg
     5101 ctgcatcgat tccactagga ttaccatggg cgattttttc cgaaagctgg acattttcta
     5161 atagtatttc acgagacaaa ggaaatgcta agtaatcata aaaagcacga gtgactgctg
     5221 tggcgactgc agcgcttgat cccattccac gttcagcagg aatcgtgctt tcaatggtca
     5281 gttgcaagtt tttttggatg tcatgctgtt tttttaagtg aatgaataat tttttgatat
     5341 tttttaatgc atgaggagct tcggctaaat ttcctgaata gtaggaagaa accagttgat
     5401 cgtccattgc atccagctct tcaaggaatg cggtgacttt tgttgcatag aaaggaaagg
     5461 caatcgctgg ttctccataa acaaccgcgt gctcgcccat caatatgatc tttccgcttg
     5521 attctccttg gccatagttt gccataatct aactccttct cacgtaaaag tgatttttaa
     5581 gcaaaaaata aagcgcccaa aacgagttgc gctgcttcac gaattttttt gtccactaag
     5641 ggtctcaaaa aacattcctc ctttatttta ctaaaaccat ctcagaagta aaactaagaa
     5701 tacgttttct aaagaaaaaa cacttttttc ttaagaattt tttgactttt tttcttcttt
     5761 gactagaaaa aaatacaatt gacactacta gatattgggg gtatatttaa aaaggttgga
     5821 gttaaacaca atatttagtg atgaagtgag ctggaggtaa atgagcgtga tcgttttagc
     5881 aggtacaatt ggtgccggaa aatcaagttt aacagaaatg atggctgaac actttgatag
     5941 tcaggcattt tatgaatcga ttgatgataa tgaagtcttg cccttatttt atgcaaaccc
     6001 tgaacagtat gcttttttgc tgcaaattta ttttttgaat aaacgttttg caagcatcaa
     6061 acaagcaatg aaagatgaca ataatgtatt ggaccgttcg atttatgaag attcactgct
     6121 tttccatcta aatgcagatc taggaagagc aacagaaaca gaagttcgtg tgtatgatga
     6181 actgctggaa aacatgatgg aagaacttcc ctatgctgcc cataaaaaac atccggattt
     6241 attagttcat attcgtgtat cttttgatac gatgttggag cgtatcgaaa aacgtggtcg
     6301 ttcgtatgaa caattgtcct ttgatccaag tctatacgat tattacaaag aattgaacag
     6361 acgttacgat caatggtatg aagaatacaa agaaagtcca aaaatccaaa tcgatggaga
     6421 ccgctataat tttgtggaag atcctcaagc aaaagaagaa gtcttgaaaa tgatcgaaga
     6481 aaaattagca gagattcgcc aaaccaaagt agccatttga tttacagttg ctcaaataaa
     6541 gggtaaacct ttatttgagc gacttttttc tttatactgg aaacaaatgt caggaggata
     6601 gagtatgcgg gaaaaacact attttaatca agaccgtaat ttttggatca atgtacagca
     6661 aaacgatgtg ctagctgtta aaaacttaaa agaagaatat ggcattagtg acgagatgct
     6721 gacttattct ctagataaaa atgaacgggc gcgcgtagag tacgattctt ttgatgaagc
     6781 gttactgctt gtatcaaatg ttccgcacca gcaaaaagtc gataatcatt atgaaactag
     6841 tcctatcgct tttattttaa aagaagacgg tttgtttaca tttaccacgc cgaataccga
     6901 gtacgtgatt cgactaattc gatcgctttt ggaacgaatg ccagacatga gtgtatacag
     6961 tttattgttt cgtacattgt ttttgatttc tgattccttt tttcctttaa tcgaggaagt
     7021 gaatagtgaa cgtcagcggc ttaatctgaa attaagagaa aaaacaacga acaagaatct
     7081 gctgcaactt tcagatttag agatcggttt ggtttactta gttacaggaa caaaacaaaa
     7141 tgtagtactt ttagaacaaa tcaaggcatt ggctatttat cgcaagttgt cagaaaagga
     7201 aaaagaagag ttggacgatg cgctgatcga agcacgacaa gcagttgaaa tgacgaactt
     7261 agcttcgcag attttagacc agctctctgg cacttataat aatttgctga ataacaactt
     7321 gaatgacacg atgaaatttt tgacggtctg gtcgctgatc ttgaccgttc caactattgt
     7381 gacaggattc ttcggaatga atctccagtt accctttacc cattccgttt tcggatgggg
     7441 aatcgccttg atcatcagtt tggtcttatc tatttggatg cttattgcac tgtggcggcg
     7501 gattcgttga tctagtcttt gatctgcaaa ccaagggctt gggcgatcac aagtgcttgc
     7561 tcttggttga cgattagtcc cgccaaagaa tcttgagata aagcgattgc tgtgaagaaa
     7621 ttagtcgtaa agtctaatcc cttcagagaa gttcgaaacc agttcgaacc ggtcaactga
     7681 ttgtcagaca aaaaaaggtt tttccaagtg acctcataaa attcgctgtc ttctaattga
     7741 caatgatcga aggagacagc tttcaagttt gtactactaa aggaagccat cgaagcaata
     7801 caatcagtga aagtacagtc tcgaagataa ctttcggcaa aattcgtacc tgttaattta
     7861 cattgatgaa acacgacttg gtggaaactg ccaccaatcc actccagatt ggaaaaatca
     7921 catttttcaa aaatgacatt actgcattca aaatgacgta aaactgtttt ttgcatcgta
     7981 agtttttgga tatgactctc tcttaatacc atatttttgg catcgagata gctttggtca
     8041 cccccgttga tcagaagacc agagaagatc tcctcatctt ctaaataaaa ctctcccttt
     8101 gtaagaagag gaagagaagg tggaataggt tcgatttttt tcatgtgggt tcacctcgtt
     8161 tttctttagt atagcaaaga agcgggaatc actgctagaa agtctgctaa tctaatcatt
     8221 ttcagaaaag gaagacaaac tgtatgaaaa acattgacaa aaagattagt ttttgctata
     8281 ctatgatggt tgagaaataa aagcagaagc acccgcttct cgccttagtt gataatgtca
     8341 ctgggctaga tagttttcac tggttctatt aggtgaagta cgcgcgggtt ctgtaagaga
     8401 actcgttttt ttattgccgt ttattcggta agcttttctt tctctcataa tatttggagg
     8461 tgaatgacca tagcaaagga tatgatggtt aacgacggca ttcgtgcacg agagttacga
     8521 ttgatcggtt cagatggtga gcaattagga gttaagacaa aagcagaagc attgaatatt
     8581 gcagaacaag caaacttgga tcttgtgtta gttgcccctg gtgcgaaacc accagttgcg
     8641 cgaatcatgg actacggtaa ataccgtttc gaacaacaaa agaaagaccg cgaagcgcgt
     8701 aaaaaacaaa aagtgatcaa tgtaaaagaa gttcgcttaa gtcctacgat cgacttgaat
     8761 gacttcaata caaaacttcg taatgcacgt aagttcttgg aaaagggcga taaagtgaaa
     8821 gcttctatcc gtttcaaagg ccgtgccatt acccataaag aaattggtca aaaagtcttg
     8881 aatcgcttag ctgaagaaac tgcggatatt gcaacagtgg aacaaaaagc gaaaatggac
     8941 ggacgcagca tgttcttgac gctggcaccg aaaaacgaca gtaagtaatt tgaatgacta
     9001 actggctgtt ggacagccga tagtcggata acgaaagatt ttgaggagga aatatagtca
     9061 tgccaaaaca aaaaacacac cgcggatcag caaaacgttt caaacgtact ggtaaaggcg
     9121 gattgaaacg cttccgcgcg tttacaagtc accgtttcca cggcaaaaca aaaaaacaac
     9181 gccgtcaatt gcgtaaagca agcatggttt ctagtggcga cttcaaacgt attcgccaac
     9241 aactagcaaa aatgaaataa gaagtacaaa taacagaatc tgaagattta tttttattag
     9301 gaggaattat acatggcacg tgttaaaggt ggaacagtaa ctcgtaaacg tcgtaaaaga
     9361 gtgctaaaat tagcaaaagg ttactacggt tcaaaacaca ctttatttaa aacagcaaaa
     9421 gaacaagtaa tgaattcata caactacgca tatcgcgatc gtcgtcaaaa gaaacgcgac
     9481 ttccgtaaat tgtggattgc tcgtatcaac gcagcagctc gtatgaatgg cttgagctac
     9541 tcaaaattaa tgcacggttt gaaagttgct gaaatcgaca tcaaccgtaa aatgttagct
     9601 gacttggctg ttaacgatgc agcagcattt actgcattgg ctgaccaagc aaaagaagct
     9661 ttggctaaat aagctaaaca attataaaga ggaaagaaga taacgaaaag ttatgttctt
     9721 tcctcttttt ttatttacaa tcctccatca aatgaaacag gacaataacg tataagtcta
     9781 aaatagcata attaaggttt tttaattgaa aactgaacat ttatggtcac cctttaatga
     9841 aagcgctaac tacataagtt agaatgagtt tgactgatat attagtttgc ttatcagaaa
     9901 aacaaaatgg acggaggaat aacatgagtg atattattat tggtattttg ttagttatct
     9961 cattcttttt catggtctgg tactgtgtaa aaggctacaa cttgatggtc ggctttgcca
    10021 tcatggctac tgtatggatg ggattagcgc tagtcggaaa cacgttttca ccaaacccgg
    10081 cgatggaagg gcaaggagta attgatgtgt tgacgcatat ctatacgacg gggccagctg
    10141 agtacgccaa atcgatcttg gtaaatgtct tctttggtgc ctttttcggg cgtgttctag
    10201 tagatagcgg aatcgcagct accctgattc gaaaggtagt tgaacttgga ggggacaagc
    10261 caagaatcac gatgagctta ctatgtattg taaccgctgt tatattcatg tcgatgacag
    10321 gaatcggacc agtgatttct attgcagtta ttgtgttgcc aatcttgatg tcattaggta
    10381 tttcagtacc ggttgcactg ttctcattta tgggctcgat tatggctgga atctttgcta
    10441 atatcgtcaa tttcaaacaa tatcaaacga tttatgctgg tttcaatcca gcggctgaaa
    10501 gctatactta taatgactat ttccaaattg gtatgattgg gatgattgta agcttagttg
    10561 tggtactaac ggtcgctaat atatcaatga acaaaaagaa acgctatgca atggcagcaa
    10621 acgtaccagc tgaaggtggc gacgcaccaa tgatctcatg gttagccgta ttattaccag
    10681 ttttaggggt cgttctttta gatcttccaa tcatcttagg ttttattcta gcaggtatct
    10741 gggcattgct gtttactgga aaattgcgcg gaggttataa agaaatctgt cgtcagtttg
    10801 cgaaattatt tacagatggc gcagtagatg ttgcgccaat ggttggtttc ttgatgacct
    10861 tagcgatgtt caataattca gctgcgtatg cttctcctta tttttcagct atttttggag
    10921 attggatacc gcaatcacca ttagtcttgg caatcgtatt tgctatattg acaccactag
    10981 gatttttccg cggtccaatg aatctagtcg gttcaggctc agctatttta gctgtcgtat
    11041 tagctgttaa tccaacgatg tcgccagcct ttttgttccc gttatttgca atcacaacga
    11101 ttgctcctca gcatttagat attacacaat cttgggttgc ttggggatta ggttatacaa
    11161 aagtaacttc tcgagaatat atgaagaaat ccatcccaac tggttggatc atcggagcga
    11221 tcttatgcct gatcacgttc ttattgtacg ggaacgccta gtaaacaaaa cgtcgataaa
    11281 aacgattaga tgaatggagg aaaagaaaat gaaacgtttt gtgcgaatgg gaattgatgt
    11341 tggcggtaca catacgaaag cagtcgcaat cgataatgcg acgcacgaaa tcatcggaaa
    11401 atcttcagtg aaaacaaccc acgatgatgt ccgtggagta gccgcaggtg tcgttcagtc
    11461 ttttcaaaat tgtttgctgg aaaataatat cagtccagaa gatgttgtgt ttgttgcaca
    11521 ttctaccaca caagcgacta atgcactgat tgaaggagac gtagcaaaag tcggcgtaat
    11581 cgggatggca aaaggtggat tagaaggttt tttagcaaaa cgacaaacta gattgaacga
    11641 tatcgattta gggaataaga aaaaaataga gattgtcaat gcattcttac ctgtcaaaca
    11701 cttaaatgta gaccgtgttt cagaaacgat ttcttcttta gaaagagaaa gagcggaggt
    11761 cttggtttct tctatggcat ttggagtaga taatggggaa ccagaacgag ttgtttatga
    11821 agcagcatcg gtaaaatcga tccctacaac gatggcttct gacatcacaa aattatatgg
    11881 attgacaaga agaactcgga cagcagcaat caatgcatct attttaccaa aaatgctaga
    11941 tacggcaacc tctacagaag attccgtacg agaagcaggg gtcaatgttt ctttgatgat
    12001 tatgcgcgga gacggcggag taatggaaat caatgaaatg aaaaaacgac ctgttttaac
    12061 catgctttcc ggtccagcag catcagtcat gggctctttg atgtacttga gagcgtctaa
    12121 tggtgtttat tttgaagtag gcggaacaac aaccaatatc ggggtcatta aaaatggacg
    12181 tccagcaatc gattattcaa ttgtaggtgg ccacccaaca tatatctctt cattagatgt
    12241 tcgggttcta ggtgtggcag gcggttcgat ggtacgggct aatcagtcag gaatcatcga
    12301 tgtcggtcca cgttctgcac atattgccgg tctagattat gcagtattta cggagacaga
    12361 aaaaatcaaa ggtcctaagg ttgaattttt ctctcctaaa gaaggagacc ctgctgatta
    12421 cgtcaaggta gtgatggaag acggagaaga agtgactatt acaaatacat gtgcagccaa
    12481 tgtccttgga ttagtgcaag aagaacattt ttcttatgga aatgtcccat ctgcacgaaa
    12541 agccatccaa gcattagctg attattgtca tacgacggtt gaagacatcg cagaacagat
    12601 catggaaaaa tcctatgcta agattgaacc ggtgatttta gaattggcag acaagtatca
    12661 cttggagaaa gatcagattt ctcttgtagg tgtagggggc ggcgctgcct cattgattac
    12721 ttactttagt aataaaatgg gtgtgaaata ttcgattcct gaaaacgccg aagtgatttc
    12781 ttctattggt gtagcacttg caatggtccg agatgttgta gaacgtatca tcccttcgcc
    12841 aagtaaagaa gatatacgat cgttgaaaaa tgaagcgatg aataaagcga ttgaatcagg
    12901 cgctacacca gaatcgatcg aagtccacgt agaaatcgat ccacagactt caaaagtaac
    12961 ggctatcgct actggttcta cggaagtgaa agcaaccgat ctaaccaagg agatcacaac
    13021 agaagaagca ctagagcttg ctgcagagga tatgcgatta aataaaaatg aagtttgctt
    13081 attggaaaat acacctttct tttatgtatg cggcgaacag aatcgttcaa aaaatgctgg
    13141 aagtcttcgg atcatcgatc aaaaaggatt tatcaaggtc caacgtggac atgcttcatg
    13201 tatgaaaaca actgcagcca attatatgac agctgtcgaa caactttggg aagatatggc
    13261 agtttatcaa actgaattga ttgcccgacc agaattttat ctctgcttag gagcaagagt
    13321 ctctgatttt acagcgacgg atctggaaca actacagctt ttgatggatc tggaagtatc
    13381 tactatggaa ccggaggaag aagtgatcgt cgttgccgga aatatcaaac aaacataagt
    13441 tagaagagtt actaaacgaa ttacaacaga tccccgaaga aatttgggga ttctaccagt
    13501 ttcggcgaga tttattttgg aaaaagattc ctttgtccaa gcagaaaatt cttatacaac
    13561 aatcaatcga ttgtggtatc gaaacggcat gtagcatcaa aaaaaagtac ccttttgccg
    13621 atgtaggaga aatttgtgaa caaatggctg ttccaatcgt ttcctgtgaa tcagagcaaa
    13681 taaacgaacg aatcactttt gcaacttatg cagaagatga agggattcga ttgatgacgg
    13741 agccgttaga aaaattaaaa tgttcaggac tagcttcgat ctctaaagaa acagcgcaag
    13801 ctttgattat tggacatgaa ctatttcacc acatcgaagc ttctgttaag ggaatttata
    13861 cgcaaagcga aaaaattgtg ctgtggcggt tgccttttta tacacatcaa tcaaacattc
    13921 gtgcattgag cgagatagca gcgatgagtt tttcaaaaga gatgaatcaa agcgattttt
    13981 cgccttatgt attagaggca gtcttacttt ggccatataa tgaaattcgt agccaaggga
    14041 tcttagaaga aataaaaaaa atagaaaaaa gatgtacgga gtatgattta gattacaaat
    14101 aaagagactg tgacaaaagt cgtgtcacag tccttttttt ccgaataaac gatgttctcc
    14161 tgtcaactcc tcggtcacaa gtcaaaatat tcaacaaaca tgagaagctg ttttttgaaa
    14221 ttgttccttg tgactcggag atgaacggca ggctcacgac ctcttggaaa acggtgttca
    14281 aaagcttgca tctgcggtaa taagtccagc caagttaaaa acataagaag ctatttttac
    14341 ttggctgttc ttattacttg atgcagggca gctttttcac aacctctcaa taaacggtgt
    14401 gcaatcagct gaccctcggt attagctcaa aaaaaacggc aaaatatgag gaactatttt
    14461 gccgtttctt tctctaatac tcaggtcaaa acgctgattc caaaacctct tatttgatgg
    14521 gtctgtcctg aatatcttca aaataatcgg ggtaattatg aatcacgtat tcttgctcct
    14581 cgataatcat atgtaaatgc atcgtgcaaa gaaatccgac ttcatcaaag ttatgatcaa
    14641 tcattgattg aagcattgtc tgatgttcat ctaatactcg accccagtct aattctgaaa
    14701 tcgtcaaacg taaccatctg aaacgcgtac tataactttc tagccaatcc caaatttctc
    14761 gttttccagc aatttcataa caagttcggt gaaaatcagt atccaaatca aaaaaagctt
    14821 ttttattttg agcgattact gcctgatcgg tttgttctag tattttttct agtttagctt
    14881 gatcttgctt ggtcattttc gcactgcatt cttgcaggat cggtttttcc aacttttccc
    14941 gcgtataacg ggctaatgaa gcagatcgga tatcgatttt tgaaacataa gtgccggact
    15001 gtggaatggt ttttacaagc tcttgattgc gcagttggat cagtgcttct cgaatcggtg
    15061 tacgcccgat agctagttgt tcttctaaca ctttatcgga gattttactt ccaggtgcta
    15121 actcggaata aatgatttgt ttacgtatag attcataagc ttgctgttgc aaattattga
    15181 taaccattta accttccttt ctatgcagat ataatctatt ataacataaa tcgcttacaa
    15241 atcgtgtggg cataaaaaca cagtctgcaa aaaaagccct gataggcatg cctattagag
    15301 cctcgaaaag aaaaatattg aatcaatctg gtcgtacctc ttggatcggt ttgacacgac
    15361 cgccgattct tttagcaaaa gcttcggatt cttttttgtc tttgaaaatg agcagattgt
    15421 ctaagttttt ttcccgagtg cgacagccag ttttatcgat gtaaccgtgc attactttaa
    15481 cgacatacat ggggatcacc tgcttttttc ttttcattat acgaactact cgacacaata
    15541 caagaaaaat gcttagacgg tctttattaa gaatttacat catccaaaga agcgtacgct
    15601 tggaataact gatctaactc cggattgact ttgctcagct tcactaatcg ttgcgaatca
    15661 gaaagtaaaa aacagtgttt actactgcca gtagttttta attttttgtc ttcagttccc
    15721 acaatttcgt agctaaaatc caaccgtgtc cccgtatact tttggatagt tggatggatg
    15781 ctcacttttt ctccgtaatg gatcatttct ttatagctgc aatttacttc cagaacgggg
    15841 atgatgatcc cagattcttc gattttttta tagggaaacc ctagatgtac caataaggct
    15901 acccgagcct cttcaaacca gcggatataa ttgctgtgat gaataatccc catttgatct
    15961 gtttcataat aaaaaggttc tcgcacataa cctgtaaatt tctccatgac tcatgcctcg
    16021 ttttccattg attttttaca aaaataattc atccatttcg gaagtgcttc gctgatttgt
    16081 gtcggcaaaa caatatattg ttcttttgcc agctggtctc caatgaaact atgaagataa
    16141 atagctgctg caatcgtttg gtcattttga ggaaactggg ctaaaaatcc tgtgatcatc
    16201 ccagctaaag tgtcacccat gccgcccgtt gccatagcag cagaacctaa aggattggtg
    16261 tagaagtgct ggtgaccata aatttttgtc tcatgacttt tcaaaacgag cacagcaccg
    16321 atttcctgtt gcttcttttt gttttcttta tcccgttgtt cttgtatcgg tagagaactc
    16381 aaccgttgcc actccatttg atgaggtgta agtacagttt gttcgggata gggtagcaaa
    16441 agatgattag cagcaagtaa acttaaagca gagccatcaa tcaccagcca ttgttctttt
    16501 gattggtttt ctagtacttg tttgaaacgt tccaaactct tagaagaagt acccaatccg
    16561 ggaccaatca agagtacatc tgcttgcttt attacggaaa gaaactggtc agtatccgtc
    16621 caaactacaa tcattgcttc tggcagatga ctgtgaagag cactccgatt tttttcatca
    16681 gttactacag tcgtgagacc cgctccgctg tgtacggcag ctaaagcact cataatgatt
    16741 gctccgccat attgttcatt gccgccaatc aaaacgatac gtccaaatgt tcctttatgt
    16801 gaaatttttg gacgaggtgt gatgactctt ttcactagtt gttcgtttat cagcatctaa
    16861 aaacctcctt ttactcatta tagcgtatat tttttttaga gaaaaacaga taagaaaagg
    16921 aattgtcaga ttttttgaac tatttttttc tgtaatttgt ggtatcatga acacgaagac
    16981 ttaaataagt ggaggattaa ctttatgaca attgattggc aaaaagaagt agaagcacgt
    17041 aaagaagact tgcttgaaga cttgaaaaat cttttacgtg tcaacagcga gcgcgatgac
    17101 tcaaaagtaa caccagatgc accatttgga cctggacctc gcgacgcatt gaaacatatg
    17161 ttagcttatg gcgaacgtga tggatttaaa gtaaaaaatg tcgataacta tgcagggcat
    17221 attgatcttg gcgaaggaga cgaaacgcta gggatatttg gacacatgga tgtcgttcct
    17281 gcaggagatg gctgggacac agatccttat gaaccagtaa tcaaggatgg aaagatcttt
    17341 gcacgcggat caagcgatga caaaggtcca agtatggcag cttactatgc gatgaagatc
    17401 attaaagaac ttgacttgaa actatcaaaa aaagttcgct tcgtagtcgg cagcgatgaa
    17461 gaaagcggct ggggcgatat ggcttattat ttcgaacatg aagaagaacc agattttggt
    17521 ttctcaccag atgctgaatt cccaatcatc aatggtgaaa aaggaaatgt ttctttagct
    17581 ttacgcttca aaggcgacaa tgcaggagat tatgtcttga aatcttttgt ttccggactt
    17641 cgcgaaaata tggtgccagg aacagcaact gcagcacttc aagtaccaag tgcagatgca
    17701 gctatcgcga tggaagaagc attttaccaa ttcatcgaag caaatccagt aagcggaacg
    17761 attgaagcgg acaatacgta tgtaaaaatc gaattagttg gtaaaggcgc acatggtgca
    17821 agcccacaat ctggtatcaa tgcaggttct ttcttggcat tgttcttaga taactatgaa
    17881 ttcctaggat ctgctaaaca atttatccat gtagcagctg cttatgtcca tgaagatttt
    17941 tatggagaaa aactaggagt cgcttatgaa gatgaaaaaa tgggcaaatt gacaatgaat
    18001 gctggactat ttgcttttga agaaaatggt acagaggaag cgaacttcat caatatgaat
    18061 ttccgttttc caaaaggtgt aacagtagac ggattgcaaa gtgacatcga acaaacagtg
    18121 gggcaagaag gcgcaacagt tactcgaggt gctcgcgtaa tggaaccaca ttacgtacca
    18181 atggatgatc cattggtggc tacattattg caagtctatg aagaccacac tggagaaaaa
    18241 ggctacgaac aaatcatcgg tggcggaact tacggacgtt tgttgaaacg tggcgttgct
    18301 tatggagcga tgttcccagg atatactgat acaatgcatc aagcaaacga atttatgagt
    18361 cttgatgact tgttcagagc aacagcaatc tatgcagatg ccatctatcg tttggcgaaa
    18421 taattaatta aaaagaggat caagaattgc aaattatttt tgcagtcttg atcctttttt
    18481 tactttctac tgtggtattc atgataaaca tgatatagat tcaattcata ttctttctta
    18541 gcgttcgtga cgactgtatc caaaatcaac cctgttacta atgaaagaag tgctaaagtc
    18601 atcaatccag tacttaaaat cgcagaaggg atgcgtgtaa taaagcttgt cagcatatat
    18661 tcgcgaatca caggaatccc cgtgaccaat ccaaataaaa agaagaagat ggtccaaatc
    18721 ccaaagaaaa gcaaaggctt ataatcttta aacattttaa taatcatcat gatcactttg
    18781 aatccgtcag agaatgtatt caattttgat tcgctgcctt caggacgatc acgataatcg
    18841 attgggattt caacgaactt gaatttttta tctaaagcat ggatcgtcaa ttccgtttcg
    18901 atttgaaaac cagaactcat aataggaaaa cttttgacaa agatacgatt gaatcctcga
    18961 tagccggtca tgacatcaag aatttttgtt ttatacattc tagtaattgt atttctaaca
    19021 aggttatttc caaaatcgtg gaaaggtctc ttattctcat caaagtaggt tccatttgat
    19081 aggcgatcac caatgaccat atctgcttca ccagaacgta atttctcgag caaaccatgt
    19141 acagcttctg caggataagt atcatcgcca tctaccatta aataatagtc ggcatcgata
    19201 tcaaagaaca tttggcggat cacatttcct tttccttgac gaggttcttt ttttacgatt
    19261 gctccacctt gaacggccaa ttcgtatgtt cgatcggtcg aattattatc atagacataa
    19321 atatctgctt ctggtaattc tcgtttaaag tctgcaatga ctgttgaaat tgttgcttcc
    19381 tcattgtaac aaggaatcag aacagcaatt ttttcttgtt gttccatttt tcactctcct
    19441 taactataga aaacaaaagc taaattaaaa atacctcata atcaaaggaa atgctagttt
    19501 ttcccgctaa atatcattta tttaaaaaga aaatactgga atatatgttt ggaaagctct
    19561 atctaaaaaa ccacccacta caaaagaagg acatgatacc tctcttttga atagtgggtg
    19621 cgtattcgtt ctctcacttt tttgacgtat taatctgttg cacctttttt tcttgaaatt
    19681 tctaatacgg ttcctgtatg agcatctgct aaaaattcat aataaacagt ttcgccatct
    19741 tctattcgag aaagacctcc ccgataagca ctcgaccgaa cggcgaattt cctaataggt
    19801 tccttttcaa aagagatcca agaaccttcg attgcacctt cttttagaaa agatgacttg
    19861 atattttcaa gtacttggtc tgctgacaat acttttttgc gatgatacca aatcgttgaa
    19921 gctaccccgc tcaataatcc tagaccgatt ccaactgcta acccgccttt aaaatagctt
    19981 agttcgcttt cttcatacat gtggacatac ctccttcttc tcacttgtta ctccttgtgc
    20041 ttattttacc acactctcat attttctttg aaataaataa ccgctaccag aaaaaataag
    20101 ctataataaa tacgatcggt ctgcagaaaa aatcatattt tatccgcaga tcaatataag
    20161 tctgagagga gtttagtctg aaatttagac gaatagcata tggaagaaaa aacatttcaa
    20221 cgaatcaaag agttaacaga attacaagga acaagcggtt ttgaacaaga tgttcgcgct
    20281 tatatggaaa gtcatatgga accgctggta gatgagttac agttagatgg tttgggcggc
    20341 atttttggtc tgcgccatca tgaagaagcg gatgcacccc gtgtaatggt cgcagcacat
    20401 atggatgaag tcggattcat gctgacacaa atccaagaaa acgggctttt taaagtcgta
    20461 cctctaggcg gctggaatcc ttacgttgtt tctgctcagc gttttacact taaaacctct
    20521 actggtaaga attatccatg tatttcttct tccgttcccc cacatttatt gcgaggaaca
    20581 agcggacaaa aatcagtaga agtaaccgat attttgttcg atgctgggtt tgaatcaaga
    20641 gaagaggcaa tgagttacgg tgtccttcca ggagacacaa tcgttcctta cgctgaaacg
    20701 atcaaaacag ccaacggaaa gaatattatc agtaaatcat gggacaaccg ctatggctgt
    20761 acaatggtat tagaagcttt agaagcgttg caaaatgaaa cattaggtca tacattgatt
    20821 gcaggcgcga atgtccagga agaagtaggc ttgcgtggtt ctaaagcttc tgtcaataaa
    20881 ttcaagccag atctgttctt tgcagtagac tgctctgcag cagatgatac agtgacgaaa
    20941 aatggcacat tcggacattt aggagaaggt acattgatgc gtatccaaga tccaggtttg
    21001 atcatgcttc ctcgtttgcg cgagtacttg ctggatatcg ctgaaacaaa caatatccct
    21061 tatcaatact ttgtttcaaa aggcggtaca gatgcaggtg ctgctcatac acaaaacgaa
    21121 ggaatcccaa gtacagtgat tggcgtagtg ggacgttata tccatacgca tcaaacgatg
    21181 ttcagtattc gcgattttga agctgcacga gaaatgttga ttcaaacact gaaaggtttg
    21241 gataaatcaa cagtaaatac gatcgtatac ggaaaataat gcgatagtga aactataaaa
    21301 tctggcaaaa gattgctttt tgccagattt tttttataaa aagaggatca atgtttgtta
    21361 gttcgaaaga aaggtgtata atttaattga aaaatacata aacagaaagt agaggacaaa
    21421 gaatgattat cccaaatagt ttagaagaat tagcaggcta cgtagaaact ggaaagagtg
    21481 tatttttctt tactgccggc tggtgcggag actgccgttt tatcaaaccg caaatgcctg
    21541 aaattgaaaa tgacttttct gattggcaat ttattgaagt agaccgtgac aagtacatag
    21601 atgtagccgc tgaatggaac atttttggta tcccaagttt tgtcgtgatc caagacggaa
    21661 aagaattagg tcgtctagtg aataaagatc gtaaaacaaa acaggaaatc gaatcgttcc
    21721 tagaaagtgt aacaaaaggc tgacatctgt taggaggggt caagatgaat caagaatata
    21781 agaatatttt agtaggtatc gacggtagtg aacaagcact tgctgctttc aaaaaagcgg
    21841 tagaagtggc tcgccgtaat aatgggaaag tatatgtagc aaacgtaatc gatcagcaat
    21901 tttataactt tatgggctat gcacctattg atcaaaatct agtcgatcag caaactgaaa
    21961 cagccaagga aatgatcgaa gaatgtaaag aatatgggaa atctgttgat tacacaaata
    22021 tagaaggaat tattgcatat ggttctgttc gtgaatccat ggcagtcaaa ttgccaaaaa
    22081 aatatgagat cgatctaatc atggttgggc agtcaggtct aaatgcagtc gaacgcttta
    22141 tgacaggaag tgtagccagc tacgtgatac gtcattcacc ttgtgatgtc ttgatcgttt
    22201 ctgacgaaga agaacagcca agtaattaag caagtggaac ttgtacgacc tttttaatca
    22261 attaactaaa acagtaaagg gactgtgaca aaagtcttgt cacagtccct tgttacgaat
    22321 aaatggtgtt ccagaagagg cttcttcgga aataaagcat gaaaaaaatt tcatgcagtg
    22381 aatgcaattt tttgttaaga taaatctcag caaaggataa tggaggaaag aacatgattt
    22441 ttgcttataa caaagcacac gtcggagaca ccttgatggt tattatagcc gacgataaag
    22501 gaacagaaaa cactgttgaa cgcaaatgga atgttgctcg gatcaaagat gaacaaggtc
    22561 aagttgttgc ttggaacttt ttccatatct ccgatcatct aacaatcgaa gggaacggcc
    22621 aagtaagctt aacagatgag gaattagcga aattgaatcg attgatcaaa gaagcaggat
    22681 tcgaagaaca gcttgaagct gatcatgaac cgaagatcgt tgtcgggtac gtaaaaacat
    22741 gcgtcccaca tcctgattct gaccatttat cgattactga gacagaagta gacaatggta
    22801 cagtattgca aatcgtttgc ggtgcaccca atatcgaagc tggacaaaaa gttgtcgttg
    22861 caaaaccagg agctatgatg ccagatggaa tgatgatctg gccaggaact ttgcgagggg
    22921 tagatagcta tggaatgatc tgttccgctc gtgaattgca tttgccaaat gctccagcaa
    22981 aaaaaggaat cttagtattg cctgaggatg ccgtagtcgg agaagcattt ccaaaataaa
    23041 gaggttgtga caagtctttg tcacaacctc ttttttatta attttcacta ttttgatttt
    23101 gttttaatgc cgattgatcg atcgtcaatt caacagtagt tgttttttct ttcgagcctt
    23161 catagtaagt gattttgaca gaatctccca cttttttctt atacaaagca gattgaagtt
    23221 ctacaccaga agaaacttct ttgtcatcga tcttagtaat gacgtcgtat tgtttcaatc
    23281 cagctttttc agccggtgta gctgtttgga catttgtcac gatgacaccg ttagttacag
    23341 atgaaggaat cttcaagatt tgttcttgtt gctgagtaga aacggcagat aagtcgacca
    23401 ttgtgatacc tagagctgga cgtgtcactt ttccgtcttt ttccagttga ttgatgatat
    23461 tcactacatc gttacttgga atagcaaatc ccattccttc tacgcttaca ttagaagtag
    23521 attcagaagt acttgcgatc ttacttgagt tgataccgat aacttgtcct tcgatattaa
    23581 ctaaaggacc accagaattt ccagggttga ttgctgcatc tgtttggatc gcattgatat
    23641 tgactgtttc gttcgattca ttcgtacttg tcacttgtcg gttcaaggaa gaaatgattc
    23701 ctgaagtgac agaatttgca tattcagatc ctagtgggga accaatcgca atcgcaggct
    23761 caccaacctt cagagcactt gagtcaccaa aagaggcaac tgtttcgact ttgtctgaat
    23821 tgatcttcaa caccgctaaa tctgaataag catctgttcc aactaattca gcttttacct
    23881 tcgtaccgtc tttcatcaag acttcaagac cttgctggcc atctactacg tgattattcg
    23941 tgactacata agcagtattc ccgtcttttt tgtagatcac gccgcttcct tcactataag
    24001 cttccaaatt actatcatct gagctgctac tttcttcttg ctgcccgaat agtccgccaa
    24061 aaccgcttga ttggttttga ctttgtaagt tgataacaga aacgacagcg tcttgaactt
    24121 tatctaccgc tttggtgata tcagaatcta cattgacttt gacattttcc acaactgttt
    24181 caccggcaga attctgattg ccgcttgtag cagtagaact attgttgcca gtgcccatcg
    24241 ctgcataaaa aatgccagct gtcagtagtc ctccaacgat ccctccaaca agccccagac
    24301 ctaacttacg ccagaggcta ttatttttac tttttttcat ctttggtgtc acattttttc
    24361 tatccatgtg ttcctcctcc aattacattc atttatctac tgttagtata gtcttatttg
    24421 ttgactttag tctactttga gactgtgaac tttttatgaa aaactttagg aaaaatcatt
    24481 gttttcagat gaaaatattt gattttcttt ttccacagat actgtgtaaa actacgcgga
    24541 taggattgga aaaataaggg ataaaacata tccacagcct gtgtataaag tggataaatt
    24601 cttataaaac gtatgtttgc ctaatgaatg tgatgaaata aggtaaaata acactgtttt
    24661 ctgtggataa acctgtggat gttgtgcata actgttgggg aaatcttttt aacaaaaata
    24721 gccaaaataa acatatagac gaaaaaattg attataataa acaaagtgag catgaagacc
    24781 aaaggagaga aaacatgaat atcaaaatta tctcagtagg aaaattaaaa gaaaaatacc
    24841 tgatccaagg tatcaatgag tatgtcaaac ggctgaatgc ctatgcaaaa atcgaattga
    24901 tcgaagttcc tgacgaaaaa gccccggaga atttaagcga agcgcaaatg agacaagtaa
    24961 aagaaaaaga aggggaacgg atcttagcga aaatcaaaga acaagaatac gtctatgcct
    25021 tagcaattga agggaaaaat cctacaagtg aatcttttgc taaacaaatc gaccaattag
    25081 gtatccaagg aaaaagccac cttgtttttg ttatcggcgg ctctttagga ctcagcgaag
    25141 ccgtcatgaa aagaagcaat gctcagatct cttttggaaa aatgacgtat ccacatcaat
    25201 tgatgcgtct gatccttgtc gagcaaatct accgtgcctt ccggatcaat gcgggggctc
    25261 cataccacaa ataatgatat tttttaagag aaaaaattat ccctatttta aataatagca
    25321 ataggtagtg gtagggagaa atttataaat aactttttta tttgctttgg tagccaagta
    25381 agttgcaaaa gaaaaataga tactgttaaa aagataatgt agacatagaa acaaata
//
LOCUS       DAYYME010000028        25126 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000028 DAYYME010000000
VERSION     DAYYME010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.028"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(291..1787)
                     /gene="lysS"
                     /locus_tag="ACHBFW_000955"
     CDS             complement(291..1787)
                     /gene="lysS"
                     /locus_tag="ACHBFW_000955"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFM9317280.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1898..2902)
                     /gene="dusB"
                     /locus_tag="ACHBFW_000956"
     CDS             complement(1898..2902)
                     /gene="dusB"
                     /locus_tag="ACHBFW_000956"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFM9317281.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2903..3790)
                     /gene="hslO"
                     /locus_tag="ACHBFW_000957"
     CDS             complement(2903..3790)
                     /gene="hslO"
                     /locus_tag="ACHBFW_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFM9317282.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4007..6118)
                     /gene="ftsH"
                     /locus_tag="ACHBFW_000958"
     CDS             complement(4007..6118)
                     /gene="ftsH"
                     /locus_tag="ACHBFW_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFM9317283.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6208..6753)
                     /gene="hpt"
                     /locus_tag="ACHBFW_000959"
     CDS             complement(6208..6753)
                     /gene="hpt"
                     /locus_tag="ACHBFW_000959"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFM9317284.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6753..8198)
                     /gene="tilS"
                     /locus_tag="ACHBFW_000960"
     CDS             complement(6753..8198)
                     /gene="tilS"
                     /locus_tag="ACHBFW_000960"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFM9317285.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8318..8788)
                     /locus_tag="ACHBFW_000961"
     CDS             complement(8318..8788)
                     /locus_tag="ACHBFW_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFM9317286.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8834..9259)
                     /locus_tag="ACHBFW_000962"
     CDS             complement(8834..9259)
                     /locus_tag="ACHBFW_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFM9317287.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9326..9595)
                     /locus_tag="ACHBFW_000963"
     CDS             complement(9326..9595)
                     /locus_tag="ACHBFW_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFM9317288.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9606..11201)
                     /locus_tag="ACHBFW_000964"
     CDS             complement(9606..11201)
                     /locus_tag="ACHBFW_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM9317289.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11216..14737)
                     /gene="mfd"
                     /locus_tag="ACHBFW_000965"
     CDS             complement(11216..14737)
                     /gene="mfd"
                     /locus_tag="ACHBFW_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFM9317290.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14754..15314)
                     /gene="pth"
                     /locus_tag="ACHBFW_000966"
     CDS             complement(14754..15314)
                     /gene="pth"
                     /locus_tag="ACHBFW_000966"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFM9317291.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15667..16641
                     /locus_tag="ACHBFW_000967"
     CDS             15667..16641
                     /locus_tag="ACHBFW_000967"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM9317292.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16811..18211
                     /locus_tag="ACHBFW_000968"
     CDS             16811..18211
                     /locus_tag="ACHBFW_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFM9317293.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18289..19011)
                     /locus_tag="ACHBFW_000969"
     CDS             complement(18289..19011)
                     /locus_tag="ACHBFW_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9317294.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19090..19596)
                     /locus_tag="ACHBFW_000970"
     CDS             complement(19090..19596)
                     /locus_tag="ACHBFW_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM9317295.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19719..21518
                     /locus_tag="ACHBFW_000971"
     CDS             19719..21518
                     /locus_tag="ACHBFW_000971"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFM9317296.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            22012..23400
                     /gene="ltrA"
                     /locus_tag="ACHBFW_000972"
     CDS             22012..23400
                     /gene="ltrA"
                     /locus_tag="ACHBFW_000972"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM9317297.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(23485..25059)
                     /locus_tag="ACHBFW_000973"
     CDS             complement(23485..25059)
                     /locus_tag="ACHBFW_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFM9317298.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         6998 a         5392 c         4688 g         8048 t
ORIGIN      
        1 ttagtaacat gtcaataact tttttgttat gtttttgaat aaatattttc ttctgtcttt
       61 tggacaagta ataatatacc aactaaattt agcttggtca acgaattttt tgtatttttt
      121 caagtttttt tcaataaacg attattcata acttttcttt ctttatcgtt cgttttcgtc
      181 ttctttaatg tctagaatag tcggcattag cttttgaact ttcgttatta tttattcctt
      241 tctacacagc aaaagctgcc taaggaattc cttagacagc ttttattcga ttatctcatt
      301 gttgggaata gcaaaacatc ccgaattgat tgtgcatcag ttagcaacat tactaatctg
      361 tcgatcccga tgcctagacc accagttggc ggcataccgt attctaaggc ttccaagaag
      421 tcttcgtcta caccgtgagc ttcgtcattt cctagttcac gttctttttc ttgcgcttcg
      481 aatctttctc tttgatcaat tggatcgttc agttctgtaa aggcattagc aaattcgcgc
      541 cccactatga atagttcgaa tcgatctgtg aatcgaggat cttctggatt tttcttcgct
      601 aaaggcgaaa cttcgactgg atgtccgtat acaaacgtcg gttgtgtcaa tgtttcttca
      661 acaaatgttt caaagaattc attgataaca tgtccgacac ccatagcttc ggttacttct
      721 acattgtgtt tttcggctaa agctaatgct tcttccaatg tcatttcttg ccagaagtct
      781 acaccagttt gttctttgat tgcatctacc atatggatgc gtttaaagtc actttctaag
      841 tcgacagctt gtccatcata tgtgattttt gctgttccta gtactttttc agctgcgtta
      901 cggataatac cttctgtcag attcattaca tcattgaaat cagtatatgc tgtgtatgct
      961 tctaacatcg taaattctgg attatgtgta gtatcaattc cttcattacg gaaaacgcgg
     1021 ccgatttcat aaactttttc cattccgcca acaattagtc gttttaaatg aagttctagc
     1081 gcaatacgca aatataaatc aatgtccagt gcattatgat gcgtgataaa tgggcgtgcg
     1141 gcagctccac ctgcctcatt gtgcaatact ggtgtttcta cttcaatata gccattgcca
     1201 tctaaatagc gacggatttc gctgataatt tggcttcgtt tcataaatcg gtcaaagctt
     1261 tcgcgattac tgattaaatc caagtagcgt tgtctgtagc gttgttcaat attcgttaag
     1321 ccatggtatt tatctggcag tggacgaagt gcttttgaca aaatcgtgat ttctgaagct
     1381 ttgatagaaa cttcgcctgt gtctgttttc atgatttgtc cagttacacc aaagaaatct
     1441 ccaaggtctg catgtttgaa tacttcatag gcctcatcac ctacttggtc ttttcttaca
     1501 taaatttgaa tttggccttc acgatcttgt agatgcgcaa atccagcttt cccttttcct
     1561 cggcgagtca tcatacgacc agctacacta gcggtcaatc ccatttctgc tagttcttct
     1621 tttgttcttg gatcaaatag ttcatgcaat tcttctgagt tgtgcgtgcg ttcaaatcgt
     1681 tttccaaatg gatcgattcc atcttcacgc aattgttcca ttttttgacg acgtacaagc
     1741 atttgatcat ttaaatcttc tacttgatgt tgttgttcct gtgtcacaaa agttcctcca
     1801 ttctcatgtt aatctctttc ctataatgcc aatgaaacac aaaaaaagca agccgaactt
     1861 ggaatttcgg cttgaaaatt cacgaaaaac ttagaaatca caacgtttgt ttcaatagta
     1921 ctttttccgc tttttctaca aattgatcca gcaggtcatt gatttcttgc tgtttttctg
     1981 tctgattgat tgctactttc actttcgctg ctcgaggaat cccttttaaa taataagccg
     2041 catgtgttct gaattctcta caagctactt tttctccttt gagatctgct aagcgttgta
     2101 aatgaagttt agctgtagca attttttcgg ctggtgttgg ttcaggaatc agttcccctg
     2161 tttcgagata atgctgtgtg cggtgaatca tccatggatt gcccagcgct gcacgaccaa
     2221 tcatcacacc gtctgcaccg acatagtcaa gcatccattt tgcatcttct ggcgttttga
     2281 catccccgtt ccccataaat ggaatcgtca aatgtttttt cacttctttc aatacttccc
     2341 agtccgcttt tccttcatac atctgaaccc gtgtacgtcc atgcatagcg atggcacttg
     2401 ctccagctga ttgtgctgct aatgcatttt ctacagcaaa gacgtgctct tcatcccaac
     2461 caatacgcat cttgattgtt actgggatat ttactgctga agaaacagct tggaccattt
     2521 cgtatacttt ctcaggatct aaaagccatt ttgctcctgc ttctgctttg attactttat
     2581 ttactggaca gcccatattg atatctatta tatcggcagc tgtgttttct tcgacaaatt
     2641 gagcagcttc aactagacta tctttatttc cgccaaatat ctgtaaactt aatggatgtt
     2701 ctgtttcttc aatgtggagc atctctaaag tttttttgtt tctgaaatga ataccttggt
     2761 cactgatcat ttcacatacg actaagcccg caccaaattc tttgacagtg actcgaaatg
     2821 ctgaatttgt tattccagcc atcggtgcaa caacgacacg attagggatt tccacattgc
     2881 caattttcca tgtgtgttcc acttaaatcc ctcctaaggc ttctttcttc aatccatcta
     2941 attcttcttc gctatacttg tatttgttat tacagaaaga acaaaccgct tctgcaccgt
     3001 gatcttcgtc tatcattgct tggatttctt ctgcacctaa cgcgataatt gcttctgcaa
     3061 atttttcttt tgaacagtca catttgaatt gaacaggcat tttttccaaa atgttcaatt
     3121 cttctgtacc aagcaatcct ttcaatattt cttctggtgt ctgtccttca tctaagaggg
     3181 tagagattct tggtgtcgtt tttaattgtt cttcgatctt gctgattgtt tcttcatcgg
     3241 cacctggcat gatttggatc atgaatcctc ccgccgtctt gatgctgtcg tcagtattga
     3301 ccaatacact cagtccaaca gctgaaggga tttgttctga gatagcaaga tagtaagtaa
     3361 aatcttcagc gatttctcca gaaacaatcg gcgtttgccc agaaaaaggt tcttttaatc
     3421 ctaagtcttt tgtgacagta aacattcctt gtgttccaac tgctccccgc acatcgattt
     3481 taccttcact atttagagga agactgagat gcggattttt gatgtaacct ttcacatctc
     3541 cctttccatt tgcatctact acaattcctg ttgctggacc atcgccttgg attcgaacag
     3601 ttagtttgtc ttctcctttg agcgtcgcac caagtagcag actaccgacc aatgtccgtc
     3661 ccaaagcagc tgaggaggtg tgccaagtat catgacgtct ttgtgcctcc gctacggtat
     3721 ttgttgcttg aacggcgtat gctcggacga atccgtcgtg agcaagtgcc ttaaccaaat
     3781 aatctttcat ttgttatgta ctcctttttc cgtttttaaa ctcatagtga atcatactag
     3841 caactactgc ttttttcaac tttggaatgc ttcttttaaa aataattcag aatcatctag
     3901 aaaacaggac atcaccctat ttacaactta aataaagaaa tctcccgaaa accggttttt
     3961 gttccggttt tcgggagtca tatcagatca attgacctat atttttttat ttgtattgat
     4021 cgtctgaatg attatcttct gattgttgag aatctgaatc atgttctgct ttattttcct
     4081 cattcagttc atgttttgct tcttcaaaat cacgtttttc ttccgcttgt ttttcagcgt
     4141 ctttttcttc caaagcacgt tttgcttctt caaaagtttt cgcttctttt tcgcttggat
     4201 aatcatttga ctcagtacct tcaggcatca caccgtgttc aaacaatgac ttgattgctt
     4261 tggcatccaa tgtttcgaat tccaaaagtt tttcagcaat cagtttatgc tgtgcacgat
     4321 gagcttcgat gatttctcga gctttatcat gagcttccat caagattttt cgtacttctt
     4381 gatcaatctc gaaagcgact tgttccgagt aagcttttgt ttgaccatag tcgcggccaa
     4441 caaacacttg atggtttcct tcatattgaa caggacctaa acgatcactc attccatatt
     4501 ctgttaccat gcttcgtgct agagctgtcg cttgctcaaa gtcattggaa gcaccagttg
     4561 attggacatt gaagatgatt tcttcagctg tacgtccacc aagtaagcct acgatttgtt
     4621 caaacatatc ttctttcgtc atcaagaact gatcttcttt tggtaaagcg atcatatatc
     4681 cgccggcgcg tccgcgaggg atgatcgtta ctttgtggac gacccgagcg cgactcaata
     4741 ctaatccgac aatcgtatga ccagcttcgt gataagcaac catttcgcgt tcttttttgc
     4801 tgattacgcg atctttttta gctggacctg caatcacgcg atcttccgct tcatcgatat
     4861 cagaagcatc gattttcttt ttatttcttc tagcagctac taacgctgct tcattcaaga
     4921 cattttctaa atctgctccg gcaaaacctg gtgtctgttg agccacaact ttcaagtcaa
     4981 cgtcatctgc catcggttta ttccgtgcat ggactcgcaa gattgcttca cgacctttta
     5041 catctggacg tccaactaaa atctggcggt caaaacggcc tggacgtagt aacgctgggt
     5101 ctagtacatc agaacggttg gttgcagcaa tgacgattac tccttcattt ccgtcgaagc
     5161 catccatttc tacaagcaat tggttcaatg tttgttcgcg ttcatcatgt ccgccgccca
     5221 taccggcacc acgttgacga cctaccgcat caatttcatc aatgaaaatg atagctggtg
     5281 cgtttttctt cgctgtttca aacaaatcac gaacacgact tgctccaaca ccgacaaaca
     5341 tttctacgaa atcagaaccc gagatcgagt aaaacggtac tccagcttct ccggcaacag
     5401 cttttgctaa taaggtttta cctgttcctg gaggtccttc taaaagtaca cctgccggga
     5461 tacgggcacc taattcaaca aatcggcgag gatctttcag aaattcaaca acttcaacta
     5521 gttcttgttt ttcttcttcc gcacccgcta catcagaaaa acgtacgcga ttagcttttt
     5581 tgtctgcttc tttggctttt gatttaccaa agttcatgac acggccgcca ccgccaccgc
     5641 cgccgccttg ttgtcccatc atcatgtaaa agaaaaagat gatgattacg attggcaaga
     5701 agctaagcaa tagggaaacc catataccgc tgcttgattc ttcttttact gttgtagcaa
     5761 ctttgttttc ttttgccagt tctgttactt ggctcaatgt tgtatcattt ggcaaaatga
     5821 ttgttgtgaa atgggtagta gtcgtatctg ttgaacctaa gatagctaac ccaccgctgt
     5881 tagcgacggt ctgctgttct ttgtattcac cagtgatctt atatacacca tttgcaggct
     5941 gcacttcaaa ttctttgatc ttgcctgctt gtagttgttc tgtgaatttt gagtactcga
     6001 tatctgacga tcgctggccg ttattaccaa aaatgaaata aacgaccatt accatcgcta
     6061 agattacgag gacataatac aaggcatttt tcatcatgcc gttgttcttt ttgttcatag
     6121 ttgccctccc taagcgtcaa tttaatttaa cagtttttta tcaatcctga ccattctatt
     6181 gtaccataat ttaattgaga tacagcatta atttgattga taaacactag gtttcaatac
     6241 accgatgtac ggaaggttac gatatgcttc tgcataatcc aagccataac cgaccacaaa
     6301 ttcatttggt acgttgaagc caacataatc tgcttcgata tttactactc ttccttcagg
     6361 tttgtcaagt agtgtaacaa ttttgactga ttttgctttg cggtatttga aaagatccac
     6421 taaataagca agtgttcttc cgctgtcaat gatatcttca acgatcaaca gatcgcgacc
     6481 ttctacattc gtatctaagt ctttgacgat ttttacttcc cctgacgaga ctgttgcatt
     6541 cccgtagctt gaaacatcca tgaaatccaa ttccaaatac gtatcgatag aacgaacgat
     6601 atctgccatg aatggtaccg ctccttttaa gacacctact actaggggat tggtgttttg
     6661 gtaaatttct gttaactctt tgcctagttc tttgcaacga acttgtatct cttcttgtga
     6721 gatcaaaatt ctctcaatat ccttttccaa catcaggttc tccttccaat tgcttctttt
     6781 tgatatttgt aaagaagtat gtagtgtatt ttatcagttt cttcacgaat actcaaatat
     6841 gaaaaggcaa aaggtatcaa gctatatata tttcttttgg catctgtaat gatccaagca
     6901 tgctgccttt gtgaattagg tactttctga tcgataaaat atctagatac tttttttgtg
     6961 agattctctg ttaatttaat gcggtctcct gcttttcttt tttcgacata cagttgcaca
     7021 tcagcaggaa tccaaatgtc atgagaaaat agagaccatg aatctgttgc tttgatgtcc
     7081 cctgcatttt caggtttgac cagaccaatc cattctgttt cattcaaata tattccttgt
     7141 tctggaatca gcaacaagcg ctggtcttct gtctggctag ttgcttgttt ttctttttta
     7201 gagagtacaa caaaatgata ctctcttttc agtatccaat tattgccaat actgagttgc
     7261 cattggcttt ttttctgttg ccactggtct aaaatcgact ccatttgttg ttctttcaaa
     7321 ataattcctg tttttgagaa tgctttgtcg aaaaaggagt aaaaaaaata gtatcgttca
     7381 gcatctgtca tttcttcaat catttctgca gaaaatcgaa attgttcctc ccactgttcg
     7441 acttcctttt cgatcaactg tcccatatat ttttgaatga cttggtctgc ccattggatc
     7501 tgttgcgaaa attgctggaa atgtctcaac gcctgtgtat tttcttgttt caattgagga
     7561 actaccaaat gacgaagccg atttctctgg atatctaaca attggtttgt ctgatcctca
     7621 aagtaaacaa gctgcgattc cgctgcataa gtatataggt cttctttgct aaaactcagc
     7681 aacggacgaa tcagtctgcc cgtagcaaat ggttgtactt ctttgattcc agaaaaagta
     7741 tttaactgtc ccccacgaac gattttcatt aacaccgtct cgatttgatc atctccgtga
     7801 tgagcagtca tcaacgtgtc atattgtcct tcttccatca cgcgagtgaa ttcttgataa
     7861 cgaaagttac gagcttctgt ttctatggcg gatttggctg gatcttccca aatacttaca
     7921 tataaaggga gattcttctc ttcacaatat gagcgaagat aggcagcttc ttctgctgat
     7981 tcttctctta attggtgatt gatgtggacg atacctaaag aaaaaccgat ttggcgttgc
     8041 acttgtaaaa gtaaatggag caaaaccatc gaatcaacac cacctgatac cgctgctaaa
     8101 attctcgatc cttgttgcca gaatccttgt tcattcccgt gccgaataaa tgactgctgt
     8161 aatagcggtg cttttttccc gcctttttca acgttcatcc tttccacacc ctttcttcct
     8221 aaaaaaaaga gagcgggaaa acgtctaaaa cgacaattaa cctgccctct gttgatacta
     8281 tgacccaaat agtcgcacta tcattatttg cggcttttta gctgcgacgt ccgccacggc
     8341 caccgcgctt tccttctgtg tttcgcttca atgaagataa gcggtcatcg ctatctttca
     8401 aaaatgaact catcaatgaa tcgaagtctt ctttttgagc tgaaggttga ctgcgagaaa
     8461 aagatttttt ctgtgctgga cgatttcggt tgtcacgggg atctttttgt tgatattttt
     8521 taggacggct ttcttctcca gattcagcag gttgttcctg cgctttccgg atagatagtc
     8581 cgatttttcc atcatcattt actgaaataa ctttaactgt tacttcatct cctacactta
     8641 aaacatcatg gatgtctttt acatagccat ttgacacttc gctaatatga actagacccg
     8701 tttttccagc acctagatca ataaacgcac caaaatttgt gattcctgat acttttcctt
     8761 gcagttttgc tcctacctcg attgacataa aaaaaatgtt cctcctaatt atttcccttt
     8821 tttatattcc tgcttatttt gcagttgtag aactagttga ttgatctgtt tcatttgtcg
     8881 agctgtctgt atttgattct aatataggat aaactttttc accgtctttt gagtaaaaga
     8941 atttgcttct ggcaagttta gctacatact catcatcttt cagcaatgaa acatcttggt
     9001 gcagctgatc aacgtctgcg ttgacttctt tcatttcagt atttgcttcg actctcaatt
     9061 cgttcaattg atgcatcctt tgcatgtcat ggaaaatctg cacacctatt atcgcgaaaa
     9121 tgaccaaagc accaacaaaa attgcagcca gtctgcgtct tcgaaaaatc agttgacgtt
     9181 gttgttgctg aaattcagca tactttttct ttgcatattc cgtatctagc gcagcgattt
     9241 tattgctttt tttcgtcact tggattcgct ccaatctttt tgattgtctt tttcattata
     9301 ccaatatcgt gaaagcttgt ctaatctatt tctctagtgt ttctgtgcga ttttcagaaa
     9361 taatttcgta cattttttgt gcatcttctt ttttggttga ttcatgtaat tccgtaattt
     9421 tcacttccaa cgttttgttg ccaaactgga ctttcaaaat atcgcctact ttgacatcac
     9481 tggaagattt cgccaattta ccgttgattt gaattcttcc tttatcagct acttcttttg
     9541 ctactgatcg tcttttgatg attcgggaaa tttttaaaaa tttatctaat cgcatatctt
     9601 attcctcact ttttaatttt tttatttaat cgcaggattt tcttaccaaa aggcaacatt
     9661 agccattctc gtactgtaaa cagacgaaga cgaatcactg cataaatgaa cacagaagcc
     9721 ccgatcccaa ctcctatcac gctgacaaaa aaagcagtgg aacgatgagt tacaggacca
     9781 aatactatat tcaatacacc ataatacagc agcagtacta ctgtcataca aagaaggctg
     9841 aacatcaact tccgtccaaa atgccgttct ttccaaaaag catttagctc ttttgtttca
     9901 attcgaacaa aataccaaag cgtcacacct aatcctaaaa gggtagacag gcttgcacct
     9961 gctgttccta ataaaccggt cagaaaagaa gtagtcaatc cttttaccaa tagtccccat
    10021 cctgcgcctt ttaaagaagg gcgaaacgag ttcttgcttt gagcaatact ttgataagct
    10081 tggatgactg cagtgaaagc aatggaaaaa acaaataata caagcgctgc atttcccgca
    10141 taatctttaa acaacgcata attgatataa gggagcagca aggctaatcc caaagacgca
    10201 gctaatgcta aagcggtcgt caatctaaga tagattttgg ctgtctgtaa aaactgacga
    10261 tagatccggt tggtcaaatg acgagtcaac gcaggcagga atgtcgcact taatgcagtg
    10321 gcaatcacta aacctaattg tactaaaggc tgacctcgat catagacccc ttttgctatc
    10381 ttcgcaccgt attctgacag tccgtaaact tccaaagcat tttttacaaa gaaagagtcg
    10441 atcagctgga aaaagataag caatccacta taaatcgaaa ccagtccgcc ttctaccaga
    10501 aaacgccgga tcaagggctt tgacggctgt ttgaacaatg gaaaccgtcg gaagcttaac
    10561 gctcctccat gaatcttttg ttcatagtag tgaaggatcc catatgcgca taaaccgcca
    10621 aaaacagctc cactcattgc tgccgtccct gtttggtaca ccgtccatcc aagctttcta
    10681 aaagcaaacg cactacagac aatcacaaaa acacgaacga actgctcaac tacttgagaa
    10741 acagcagttg gttccatcaa gaatcttcct tggaaattcc ctcgataaaa agatagacca
    10801 ggcatcagca aaaaggtaaa ggaaactact tggatcagcg gtttcaattg tatgtctccc
    10861 atcatccaag caatccagct gctaaatgca aagacaaaca accacaaaaa gacacctgtc
    10921 caaaatacta agggataaag attttgcaaa gcttgctttc gctcgaaagg attctttctt
    10981 tctgcaacgt actttgatat gaactgcgga agaccggata aagctaaagt cattgctatc
    11041 ccataaattg gataaacttg ctgataaaca taaaaacctt cgtcgccgac taagttttgg
    11101 aggggaattc ggtaaagggc acttaatact tttgctatga aagaagcaat cgtcaataca
    11161 aatgctcctt gcatgacgcg ccgcatttct ttatgatcca aatcattgct ccttcctacg
    11221 ctgacagata tttttgctca cgcaacgctt tgacaaactt ttggatttcc tgaagccata
    11281 ctgcttcttc catttggttg gggatcgtca agcgtatcac catctgttct ttttcaacgc
    11341 ctagactggc ttttagtttt gtcgctgtca gtgcttcgaa taactgttcc acactatagc
    11401 gttttgttcc aactttactt agagtaaaga ctatatgcgc ctcctgtttc cttatcgttt
    11461 ctaggagcgc tcgatcgcca tccattttga tctgcccaat tgtcaagaga tgtgctactt
    11521 cttctggata ttctccaaaa cggtcgagca gatcatcttc tagttcatct agcatttctt
    11581 gcgaatcaag ttcacgaatt cgtttataga tctcgatttt ctgccgttga tctgccacgt
    11641 atgtttcagg tagatacgca tcgacaccga ggtcgatctc aacagtggtc ttctccactt
    11701 gactattctt tccttgttta cgagaaacag cttcgcttag catttgagaa tacatatcga
    11761 aaccgactgc atcaataaat ccatgctgct gagcacctaa gagattacct gcaccgcgaa
    11821 tggaaagatc ccgcatcgca atcttgaatc cagatcccag ttctgtaaaa tctttgattg
    11881 cctgaagacg tttttcactg acttcgttaa ggatcttctg ctgctcatac ataaagtaag
    11941 catatgcgac acggctgctt cttcctaccc gccctcgcaa ttggtataac gtggataatc
    12001 ccatataatc agcgttctca acgaacaacg tatttgcgtt cgggatatct actcctgttt
    12061 caatgatggt tgtagtgaca agaacatcat attgaccttc aataaattca aaaagcgtat
    12121 tttctaattg cgcctctgtc atttgcccat gagcataacc gatccgtgct tccggaacga
    12181 gttcttggat ttcttctact ttttgttcga tcgtttctac ccgattatat aaatagaata
    12241 cttgtccacc tcgtcccatt tctcgatgga ttgcttcgcg aatagcccca ggattttttt
    12301 ccataacata cgtttggatc ggataacgat tcgcaggcgg tgtttcgatt actgaaagat
    12361 cccgcacgcc taacatcgac atatgcaaag ttctcggaat cggtgttgca gtcagagtca
    12421 aaacatccac ttgtgcacgt aactgtttca atcgttcctt atgttttaca ccaaaacgtt
    12481 gttcttcgtc aatgatcagt agacccaaat cgctaaattc aatatctttt gacaaaattc
    12541 ggtgggttcc tacaacaata tcaacttgac ctgtacgcag ttgttcgatg gtttcttttt
    12601 gttgctttct tgttctgaaa cgacttaata gcccgatatt tacggggaaa ccttcaaatc
    12661 tttccagcat cgtttcatag tgctgctgtg ccaagatcgt tgttggaact aaaaaggcta
    12721 cttgtttgct ttcctttatc gctttaaagg ccgctcgaag cgctacttct gttttcccat
    12781 aaccaacatc acctacgagt agtcgatcca ttggtttttc tttttccata tcccgtttga
    12841 tctccgctgc acttctcaac tggtcatcag tttcagagta ggggaaggca ttttcgaatt
    12901 ctttttggta ggcgtcatca ggtccaaaag catatccttt ttctgactct cttgcggcat
    12961 ataatttgat tagatcgtca gcgatatcct cgatttttga agagactttt cgcttggttt
    13021 tcgtccattc acttccgcct agcttattga tgcgaggcgc tttggcttca gaggcgacat
    13081 atttttggat cagattcagt tgagtaacag gaataaacag tttgtcgtcg ttttgataca
    13141 aaatagtcat atagtcttgg tgaacgccat ctacttctag cgtttccatc ccaatgtatt
    13201 tcccgatacc atgattggca tggacaacgt aatctcctgc ttttaactca ttatagcttt
    13261 tcaatcgctc tgcgtttgtt actgtttgac gtctcgtttg ttttttcgtc gttttttgga
    13321 aaatttcttt ttctgtgata gctactattt tttcttgagg aagttcaaaa ccagcctgca
    13381 aagctccttc aactaattgt acctgtcctc tgatcaatgc atttttttct gtttctacta
    13441 ctaatatgtc ttcatctcga agcatttgtt ctgctttgcg tattctgtcc tttgtcggaa
    13501 taaagatcac aacagtctgt ttttgcttgc gccaacggtc catttctgtt ttcagcaatg
    13561 gcatttgtcc gaagaactgt tgcatcgtcc gatattgaaa attatggatt gcttggaaac
    13621 gaagatttcc catccctttt tggaagagtg agaaaaaggt tgtggtgaac ttttccttct
    13681 gcacgatttt ttgtatatct gctgaaaatg tctgctcaga aaacacccgc aattcgctga
    13741 ttttttgagt ctgccattct gccgcttccc tttcgatttc ccgatttgtt tccatgattc
    13801 gctgataatc atcaacgact agtaaactat ttgaagggaa ataatccaag atcgtcacag
    13861 attcttggta aagaagatct gagtagaatt ttgcttcttc tgtcgggaca cctgcttgcc
    13921 agcttgaagt cagctgaccg aagtaatctt gcaaaaactc gcggtctgct tcttctgtgg
    13981 tcgcagcaag ccgtttttct agcatttttt gcatttgctc tattccatgt tctagatcct
    14041 ctggtccgta gacttgttca ctcgttgggg aaacccagat ttcttccgtt ttttcgattg
    14101 agcgttgtgt ttctgcttca aaataccgca tagaatctat ctctacatca aatagttcta
    14161 cacgaacagg ataggttgtc gttaacgggt aaatatcgat aatgcttcct cgaatactga
    14221 attctcccgg ttttgcgacc atctcttctc gtacatagcc catcaaagtc aattggcgag
    14281 gaagattttc cacgtctatc tcatcaccaa tatgccaatt caattgcgcc tgtagccaag
    14341 ttttcttatt cggcagtcgt ttgtgcaaag atgcgactgg caacaaatag atacctgctt
    14401 ttttttcttg aagtgcgtta agcgttgcaa ctctctcgcc tcgcgcttct ggagaagaaa
    14461 aggccatttc agcagaaagc acttcatcca ctggaaataa atggatctga tcttgcggaa
    14521 tcacatgttg cagatcttct gctaactgat tcgcataata caaatttggc atcagaataa
    14581 tcatgttttt atttgttttc ttaaacgctc gagcaaatag aagtgttttc gctgagcccg
    14641 ctaatcctgt gattagttgt ctaggcattt tttcttctaa attgctgacc cattctttta
    14701 cttgcggatc ttttccgact aattgaataa tatccattcc ttttcctcct tcctcaattg
    14761 aatcgattca tcgcatccac gaacgaatgc ccttctatga gatattcgac tgctttaaca
    14821 gattgacaca tgctttcttc tatcaaaggc tgattttctt tgctgaaagg actaagcaca
    14881 tgctggacta ctgttttttt cccttctggc ctgccgattc ccaccttgat tcgatcaaag
    14941 acattcgtat tcaaatggga aataatactt ttgataccgt tatgcccgcc ggcacttccc
    15001 ttttgacgga gccggatttt tcccactgcc agatccaaat catcgtagat tacgactaat
    15061 tcctctggat aaatcccaaa ataagtcatt agtgggccga ctgcacgacc agattcattc
    15121 atgaatgttt gtggtttgac taaaaggatc ttttctccat tatgaaaaaa ttcggcaact
    15181 tctgcttcga acggattttt tttgaaagta gcttgatgct cgcgtgcaat tttatccacg
    15241 accataaagc cgatattgtg ttttgtatat tggtatttgg tacctgggtt tcctaacccg
    15301 acgatcattt tcatttttgt tttccaccta attctaaaaa gcagcacaaa aggctggact
    15361 gctaattagc ggtccagtcc atcgagcttt tttctttttt acacttgtca atgcatagta
    15421 taacacaaaa caaggcgttt cggctaaaag agaatcgtta ataaatgata aagaaattaa
    15481 aaatttcttc aaagtttaat gaaataattg atatataaaa atagtgaaat aacgcacatg
    15541 taagctttta catataatac aaccttgtta tctgttatat tttatagcca aatagcgtga
    15601 catggggctt gagaaatgtt agcataagca tgggaaaaaa caaatcaatg gaaggattga
    15661 tactaaatga ctgcagctgc agaaaaaaaa gatcatcaaa aagttattct tgttggagac
    15721 ggcgccgtag gttctagcta tgcctttgct ctagttactc aaaatatcgc tcaagaagta
    15781 ggtattgtgg atatcaatac tgctaaaact gaaggagacg caattgactt gtctcacgct
    15841 ttggcattta cttcacctaa aaaaatctat tctgctactt acgcagacgc acatgatgct
    15901 gacttagttg ttattacagc tggcgcacca caaaaaccag gtgaaactcg tttagactta
    15961 gtaaacaaaa acctaaaaat caaccgcgat gttgtcacac aaatcgttga atcaggattt
    16021 aacggtatct ttttagttgc cgctaaccca gtagatattt tgacttactc aacttggaaa
    16081 ttctcaggtt tccctaaaga acgcgtaatc ggttcaggta cctctcttga ctcagctcgt
    16141 ttccgtcaag cacttgcgga attagttgac gttgatgcac gtaacgtcca tgcttacatc
    16201 ttaggtgaac acggagattc agaattccca gtttggtcac atgcaaatgt tgccggcttg
    16261 caaatctatg aatgggtaaa aaacaaccca gaaatcgatg aagaagctat ggttaaccta
    16321 ttctttggcg tacgtgacgc tgcttacaca attatcgaga aaaaaggcgc tactttctat
    16381 ggtatcgcag tagcattagc acgtatcact cgtgccatct tagatgatga aaatgctgtt
    16441 cttcctttat cagtattcat gaatggcgaa tatggattga acgacatcta cattggtgca
    16501 cctgctgtta tcaaccgtca aggtatccaa aaagtaatcg aaattccttt aagtgacagc
    16561 gagcaagacc gtatggctgc ttctgctaag caattaaaag aaattctaga tgaagctttt
    16621 gctaaactag acgctgaata atctttgaac atgacggaaa acatctattc ggtgttttcc
    16681 gtttttttat tttatcaccc ttatggtttc taaatttttt cataaagtag agataacctt
    16741 cgtcttttcc tctaaattat gctatcatga gaccgttcca ccctaaactt ttacttaagg
    16801 agttgtcgat atgacaagat cttcgcatcg aaaaaaatcg aaacatgttg ctttgctttc
    16861 agttcttggt gttctcttag tgctgatcag cttttatacc tatcgcagta catactacac
    16921 aaagcatttt ttgccgaata cagaaataaa tggaataaat gtcagcaatc taacagtcga
    16981 aaaagcaaat gaaaaattaa aagaagctta ttctgataaa aaactggcta ttaaagaaga
    17041 tggaaaagtc tggcaggaag taccaaaatc cgagctcggc tacaaagatg atttcacttc
    17101 ggagctttct ccgatcctaa atgaacaaaa cggatggact tggggaatga cttatgtctc
    17161 agcggcagaa aaacaagaaa tagatcctgt cagtcaaaac caccaaaaac tagacactac
    17221 cgttcagaca ttgacaacta agctgactga tttgaacaaa gaccggacac ctactactga
    17281 tgcaacgatc gaaaaagcag gtgactcttt cgttatcaag cctgaagtca atggaaatac
    17341 gatcgacgtc gatgctgcgg tcaaacaatt aaaaagtgcg gtaaactctg gtaaagatac
    17401 aattgaattg actgaattca aagaaaaacc aaaagtaaca tctgaagaca gctcactgaa
    17461 agaacaactc gcatccatga atgctatcgc aaatgtaaaa gctacttaca gtataaacgg
    17521 tgagactttc cagatcccat caagtgatat tatgagttgg ctgacctaca atgatggaaa
    17581 agtcgattta gatacagaac aagtacgtca atatgtaaca gatttaggca caaagtataa
    17641 cacaagtacg aatgacacaa aatttaaaag tacaaaaaga ggagaagtta cggtcccagt
    17701 tggcacttac agttggacga tccaaacaga cagtgagaca gaagcattga aaaaagcgat
    17761 tctggcaggc caagatttta ctcgttcacc cattgtgcaa ggtggcacaa ccgctgatca
    17821 tccattgatc gaagatacgt atatcgaagt tgatctggag aaccagcata tgtggtatta
    17881 caaagacgga aaagtggccc ttgaaacaga tatcgtttcc ggaaagccaa ctacacctac
    17941 acctgctgga gtcttctatg tttggaataa agaagaagat gcaactttaa aaggaacaaa
    18001 tgacgatgga acaccttacg agagtccggt aaactactgg atgccgattg actggacagg
    18061 tgttggtatc cacgattcag actggcaacc tgaatacggt ggagacttat ggaaaacccg
    18121 cggttcacac ggctgcatca acaccccacc aagtgtaatg aaggaattat tcgggatggt
    18181 cgaaaaagga acgcctgtat tggtcttcta acaaaaataa tatataaaaa gaaagcaccc
    18241 gtacgattgg tacaggtgct ttctttttat tatctaacaa tgtgctactt acatttcagc
    18301 agcagtaaag acttctgaaa catcatcatc gtcttctagc ttatctacta gacgttccaa
    18361 ttgctctttt tgttcctcat ttaaatcaat cgtcgtttgt ggaatcatcg tcaattctgc
    18421 ttgcgccaaa gtgaactctt tttccaactc atctcgtaca gcagcaaaat cttctggtgc
    18481 agtataaatc tcgaatactt caggagaagt ttgcagatct tctgcccccg cctctaatac
    18541 atcctcaaac atcgtctctt catcaacagt caacccatcg cgttcaatag cgatatatcc
    18601 tttgcggtca aacaagtaac tgactgaacc cgtttctccc agtgatccac cgtttcgagt
    18661 aaaagctact cggacatttg tcgctgtccg attacggtta tctgtcaatg catggactaa
    18721 gattcctact ccacccggac catatccttc atacgtgatt tcatcgtaat tcgtttcatc
    18781 tacggcactc gtagcctttt taatcgcccg ttccacattg tcgttaggca tatttgcagc
    18841 ttttgctttg tccacaacaa gccgcaaagc tggattcata gatggatcag gaccgcctgc
    18901 tttagctgcc atataaattt cacgagaaag tttctgaaag actttccctc gttttgcatc
    18961 ttgggcattt tttcttcctt ggatattatg ccatttacta tgtcctgaca tgatttttcc
    19021 tcccttgttc ttttgagaat acacttctat tctagtacta tttcacaaaa aaacaaagcc
    19081 acagccaatc tataaacgct tcttgaatgc cgcaaaaagc acacataata tcacagcagt
    19141 cagagcaccg atcgaatcca acatgacatc ttggaacata ggtgttcgtc caccggtcat
    19201 catctgatgg aattcgtcca aacctgcgta ccctgttgct gaaagccaag caaggatcgc
    19261 tgtcagccac caaattttta gcttggggaa aacacctaaa aacaagctgc ctcccaaaat
    19321 aaaataggta ccgaaatgtg cgcccttcct taaaaagaac tctaaaaatt tggcgtagcc
    19381 cagatggttg atactcacca tacttccacc ataattaaaa cgtatacccg ctaatgtgtc
    19441 tttaaatggc tgattaggca gccagctttc taatcgcgaa acttgtgatt gctgtccgta
    19501 agtttgagaa gaactgtaaa ataagattat aacgactatc agtgcgatta ctaaataaat
    19561 atttccgttt ttcatttgtt tttttgaaga attcataagt cacctctgta ttttatctta
    19621 gacttttttt gaagaaacaa caatcatttt catagagact tcttccataa aaaagtgcta
    19681 tacttaattt tgtattacaa tatgaaggga gttttttcat gagttattca ttaaattgga
    19741 atctagattc tattttttcc ggaggcagtc attctgatgc tttgaaccaa cggatgaagc
    19801 aattggaaga ccaaatgaac gagtattatc accgcgtaac aaaatggtca cctagttctg
    19861 acaatgcaga gcagttaaac gctattcttc aattgcaaga aacgattacg aatggtttta
    19921 cccaatgcag cagctatatt actgcattgc tttctgccaa tgtcaatgat tcagatgcaa
    19981 aggtcttatc aggcaagctt tacgcaatgc tgcctcgtct gcaatcagcc gaaactgtat
    20041 tgtcaaagaa atttgcagaa atatctgaca atgattggaa tcagatgctt tctcaatctt
    20101 cgtttgaaac aattgctttt cgtttaaacg agattcgtcg tgatggctca caattattat
    20161 cagaagctga agaaaacatt ataaatacgc tttctttaga tggtctaaat gcttggagca
    20221 gtcattatga tacgattgta gccagcattt cgattccctt cgaacaagat ggacaagtag
    20281 tcgaattatc tgctggacaa gccttcaata agatgatggg cgatccagat ccaaaagtca
    20341 gagaaacact tttcgcagca tgggaagaag cctggaaaga aaaggcaccc ctcttcacgg
    20401 atactttgaa tcatttagat ggttttagac tttctgacta tgaacttcat ggtgtcacag
    20461 attttctaca aaaaccatta gagtacaatc ggttgaagaa agaaacattg tctgtcatgt
    20521 gggatacaat ccagaaaaac aaacaacccc ttgttaatta cttgactaga aaagctaatc
    20581 ttttcggtaa agaaaaaatg gaatggcaag atcaagatgc acctattatt ttaggcgatt
    20641 tgaaagaaaa aacctttagt tttgacgaag cagcagcatt tattatcgaa aactttaaca
    20701 aattcagtcc aaaaatggct aaatttgccc aatctgcttt tgaaaagagc tggatcgaag
    20761 cagaagatcg tcctgggaaa cgtcctggtg gttattgtac tgagttacca gaaacaaaag
    20821 aatcaaggat ctttatgacc tacagtaatt ctgttaatga ggttgcgaca cttgctcatg
    20881 aactaggaca tgctttccat agcagcacga tgtgggattt accttcttta aatcgcgaat
    20941 atgccatgaa tgtcgcagaa acagcaagta cattcgcaga attgatcgta gccgatgcta
    21001 cattaaaagt cgcaaaaaca aaagaagaaa aaatcaattt gctagataca aaactgcaaa
    21061 atgctttagc tatgttcatg aatatccatt cccgattcat ttttgaaaat cgattctatg
    21121 aagcacggca agaaggatta gtctcagaag ataagatcac agagatgatg gttgaggcgc
    21181 aaaaagaagg ctatcaagga gctttagcta cttaccatcc atatttctgg gctgcaaaat
    21241 tgcatttctt tattgatgac gtaccgtttt ataacttccc atacacattt ggctacctct
    21301 ttagtttggg catctatgct tacgccaacc aaaagggagc aaattttgaa gaagaatata
    21361 tccatttact acgcgatact gcctcaatga cgacggaaga tcttgcgaag aaacatttga
    21421 atgtcgatct tacccaacca gatttttggc aagcaggtat cgacatggtg actgaagata
    21481 tccaaacgtt catggaattg acagaagaat ttatctgatt tctattttat cacaataaaa
    21541 aaagagttag tccagaatgg gctaactctt tttgatgtgc gccctgcatg ggtgaaaact
    21601 tggtggtgaa agtccactac aggcttggca gtaggaactg ttagcgaaag acaaggcggc
    21661 aatcgtgagg ttgtggctga aggaagtcgg acgcaaactc ctgaactgac gaacagaaac
    21721 tagatacaag gctgaattag gtcggataag tcttcatcac aagacaaagt ccaatactgc
    21781 ccgaaaccta tacagtaaat ctagcagtta catgggagga aagttttcac tcttaccggg
    21841 ggagatctca tgagggtgtt gtgggcgttg aatgaaaaca aaccagcaca acgatcatgg
    21901 cgacatgatg gtgaatcatg agaagtcagc cgaggtcata gtagtaacga cactgaccgt
    21961 tacgaaggac tgaacaataa gaatcttgaa taatttagga ggtgtgagaa gatgcgacga
    22021 ccgcagaaaa caagaaaact tggctatcat caaagagcta gtgtggaaca cgaagggtat
    22081 gatgaagtgc gcagcacttc ttgcggtgaa cagaggaaac aagatggtgt agaaaatctg
    22141 tttgggagaa ttctagatcg gaataatctc aatcaagcgt atcttcaagt agtcagaaat
    22201 aaaggggcgg ctggtgcaga tgggatgacc gtcgatcaac tgcttcctta cttgaaagag
    22261 caccgcgagg aattactatc caatttacgc caagggaact ataaacctca gcctgttttg
    22321 cgagtagaaa taccgaaacc aaacggcggt gtaagaaaac taggaattcc cacggttatt
    22381 gatcgtatgc ttcaacaggc aatcaatcaa gtgttacaac cgctatttga accagaattc
    22441 tcagataata gttatgggtt tcgcccaaat cgcagcgcac atgacgcagt gaagaaagca
    22501 aaatcttatt atgaacaagg ttaccgatat gtggtggaca ttgatatgaa agcctacttt
    22561 gacaccgtaa atcacgataa gctgatgtat ttcattgagc ggaaagtaaa ggacaaacaa
    22621 gtcctaaaac tgatccgtca atacttaatg agtggtgtaa tggaaaatgg actcatcaca
    22681 acaactgaag aaggaacgcc ccaaggaggc aatctctctc ccctgttaag taacatctac
    22741 ctgaacgaat ttgatcaagt gctagaaaat ctaggacata aatttgttcg atacgccgat
    22801 gactgcaata tctatgttag aagcaaacga gcaggacagc gtgtgatgaa gaaatccatt
    22861 caatttctcg aagaacaact aaagttgacg gtaaatcgag aaaagagtgc ggtaggaagt
    22921 ccattgaaac gaaagtttct tggattctgc attctgccaa caaagaaagg tattaaaatt
    22981 cgccctcacg caaaagcaaa acagcgtgtg aaaagcaagc tcaaacaact cactaaaaga
    23041 aaccgtggaa gaagtatcca acttatttta aaagaaatca agcaactaat gactggttgg
    23101 atcaactact atggaattgg tgagatgaaa gaattcatga gggagttaaa cggttggttg
    23161 aagcgaagaa tcagacaata tatttggaaa caatggaaga atccaagaac caaaagaaaa
    23221 aatctgattc gtttagggat cgataaagcg aaagcgtatg aatggtcaaa tacaagaaaa
    23281 ggtatttggt cagtctccaa aagtcatatt cttcatcgat cattaacaga caaagaactg
    23341 gcccgccaag gatatgagga catctccttg aagtaccagt tcgtacactc aacttattga
    23401 accgccgtat acgggtccgt acgtacggtg gtgtgagagg ggcggaaggt cagttggctt
    23461 tcccctctac tcgattaatc attcttacat gctgacagca aagtcagggg caaaccattg
    23521 aactgaacca acttttacgc tttcgcctgg ttgaggagca tggatatatt gtcctccgcc
    23581 taaagcaatc gctacgtggt aagttccgcc ttgtgacccc cagaataaca agtctccagc
    23641 ttttgcttga gaaactgaaa tttttgttcc agctgattct tgaggaactg tccaaccacc
    23701 gatgtcacga ccagttactt gcatgtaaac atagcgtgtg aaccctgagc agtcaaagcc
    23761 acttggatct ttaccgcccc aaacatatgg agtaccaata tatttgtaag cttctgctac
    23821 gattgctgca ccattcacac ttccagaagg agctggagca ggtgttacag gttttggttg
    23881 ttcaggtgtt ggtgttggcg ttggagccgg agtactactt cctgtgccgt ttcctgtatc
    23941 aacactttga tctgttgatg gtgttgtagg cgcaggtgtt gttgattctt ctgttgttga
    24001 agattcaggc actgttgttg attcttctgt tgctgaagat tcaggcactg ttgttgattc
    24061 ttctgttgct gaagattcag gcgctgttgt tgattcttct gttgctgaag attcaggcgc
    24121 tgttgattct tctgttgaag attcaggtgt tgttgattct tctgttgctg aagattgtgt
    24181 agctgatgat gattcttcag tcattgaaga ttgtgtagct gatgattctt ctgttgctga
    24241 gctttgtgca gtagatgaag cttgagtagc ttgtgcttca gcctctgctt gttcgcgtgc
    24301 ttctttttca gctttttctt gtgcagcttg ttgacgagct tgttctgcta gacgttgttg
    24361 ttcacgaata cgagcttgct ctgcttcagc ttctgctttt tgacggttca agtcagcttt
    24421 cttgtcttca gcagttgctt gttcagcagc taatgaagtt ttcagtacgt tcaaatcagc
    24481 ttgtttagat aaaagatcgc ctttttgaga ttccaaagca gcttggtttt cagctaattc
    24541 ttttagttta gcatcgtttt cagctttttt gtcttctaca gcttttttat cttgtttttg
    24601 ttgttccatc aagtcgttgt ttgcttttac catcgttgtc attgcttgaa cgcgtccaat
    24661 agcatctgct aatgaatcag cattcaatac agcatcgatg tagtttgagc ttgtgttgct
    24721 tacttgtgct tcgcgtgctt gtttttggat tgtatcttca cgtttttcaa tacgttcttg
    24781 taaatctgca atgtctttta ctaattgtgc agattcttga cgcaatgtat cttgttttgc
    24841 taataggtct tgtgcttgtg tgttgatttc agaaacttgg ctttctaagg catcgatttg
    24901 tgattgtgca tctgcttgtt gatttttcaa gtctgcaatc ttttgatctt gctgttgtat
    24961 ctgagaatca aaatcgtccg ccgcggcagc gatcggtgat gctacggcag tcaaggtcat
    25021 tgaacatacc attactgctg atattaaact ctttttcact cttcattcct ccgactggct
    25081 taatttaata aatatctaaa tatatttaag tctttcttaa cgatat
//
LOCUS       DAYYME010000029        24249 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000029 DAYYME010000000
VERSION     DAYYME010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.029"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..769)
                     /locus_tag="ACHBFW_000974"
     CDS             complement(<1..769)
                     /locus_tag="ACHBFW_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9317299.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFY"
     gene            complement(993..2933)
                     /gene="thrS"
                     /locus_tag="ACHBFW_000975"
     CDS             complement(993..2933)
                     /gene="thrS"
                     /locus_tag="ACHBFW_000975"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFM9317300.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3270..3449)
                     /locus_tag="ACHBFW_000976"
     CDS             complement(3270..3449)
                     /locus_tag="ACHBFW_000976"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFM9317301.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3496..5319)
                     /locus_tag="ACHBFW_000977"
     CDS             complement(3496..5319)
                     /locus_tag="ACHBFW_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9317302.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5597..7876
                     /gene="helD"
                     /locus_tag="ACHBFW_000978"
     CDS             5597..7876
                     /gene="helD"
                     /locus_tag="ACHBFW_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFM9317303.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(7969..8616)
                     /locus_tag="ACHBFW_000979"
     CDS             complement(7969..8616)
                     /locus_tag="ACHBFW_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317304.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8795..9514)
                     /locus_tag="ACHBFW_000980"
     CDS             complement(8795..9514)
                     /locus_tag="ACHBFW_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9317305.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9645..10796)
                     /locus_tag="ACHBFW_000981"
     CDS             complement(9645..10796)
                     /locus_tag="ACHBFW_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFM9317306.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11013..11753)
                     /locus_tag="ACHBFW_000982"
     CDS             complement(11013..11753)
                     /locus_tag="ACHBFW_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9317307.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11757..12080)
                     /gene="rsfS"
                     /locus_tag="ACHBFW_000983"
     CDS             complement(11757..12080)
                     /gene="rsfS"
                     /locus_tag="ACHBFW_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFM9317308.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12137..12730)
                     /gene="yqeK"
                     /locus_tag="ACHBFW_000984"
     CDS             complement(12137..12730)
                     /gene="yqeK"
                     /locus_tag="ACHBFW_000984"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFM9317309.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12714..13364)
                     /locus_tag="ACHBFW_000985"
     CDS             complement(12714..13364)
                     /locus_tag="ACHBFW_000985"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFM9317310.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13374..13685)
                     /gene="yhbY"
                     /locus_tag="ACHBFW_000986"
     CDS             complement(13374..13685)
                     /gene="yhbY"
                     /locus_tag="ACHBFW_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFM9317311.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13701..14810)
                     /gene="yqeH"
                     /locus_tag="ACHBFW_000987"
     CDS             complement(13701..14810)
                     /gene="yqeH"
                     /locus_tag="ACHBFW_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFM9317312.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14807..15334)
                     /locus_tag="ACHBFW_000988"
     CDS             complement(14807..15334)
                     /locus_tag="ACHBFW_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFM9317313.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15514..16299)
                     /locus_tag="ACHBFW_000989"
     CDS             complement(15514..16299)
                     /locus_tag="ACHBFW_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFM9317314.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16292..17161)
                     /gene="accD"
                     /locus_tag="ACHBFW_000990"
     CDS             complement(16292..17161)
                     /gene="accD"
                     /locus_tag="ACHBFW_000990"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFM9317315.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17162..18541)
                     /locus_tag="ACHBFW_000991"
     CDS             complement(17162..18541)
                     /locus_tag="ACHBFW_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFM9317316.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18549..18968)
                     /gene="fabZ"
                     /locus_tag="ACHBFW_000992"
     CDS             complement(18549..18968)
                     /gene="fabZ"
                     /locus_tag="ACHBFW_000992"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM9317317.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(18993..19469)
                     /gene="accB"
                     /locus_tag="ACHBFW_000993"
     CDS             complement(18993..19469)
                     /gene="accB"
                     /locus_tag="ACHBFW_000993"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFM9317318.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19472..20707)
                     /gene="fabF"
                     /locus_tag="ACHBFW_000994"
     CDS             complement(19472..20707)
                     /gene="fabF"
                     /locus_tag="ACHBFW_000994"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFM9317319.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20726..21463)
                     /gene="fabG"
                     /locus_tag="ACHBFW_000995"
     CDS             complement(20726..21463)
                     /gene="fabG"
                     /locus_tag="ACHBFW_000995"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFM9317320.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21475..22392)
                     /gene="fabD"
                     /locus_tag="ACHBFW_000996"
     CDS             complement(21475..22392)
                     /gene="fabD"
                     /locus_tag="ACHBFW_000996"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM9317321.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22499..22723)
                     /locus_tag="ACHBFW_000997"
     CDS             complement(22499..22723)
                     /locus_tag="ACHBFW_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM9317322.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22776..23738)
                     /locus_tag="ACHBFW_000998"
     CDS             complement(22776..23738)
                     /locus_tag="ACHBFW_000998"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFM9317323.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKIPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23748..24188)
                     /locus_tag="ACHBFW_000999"
     CDS             complement(23748..24188)
                     /locus_tag="ACHBFW_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9317324.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6635 a         5408 c         4232 g         7974 t
ORIGIN      
        1 tatagaaata aaaaatgata tcgatcgtag caaattgggc caaaagtgaa ttagttgccg
       61 cacttcgatt cgtgatttct tttggtctag atgtttgtac gagtacatcg gctatcttgc
      121 taagcgggtt atttccaaat tgcgtcaacg caatgatttc gatattcatg cttttcgcca
      181 gctcggctaa tgcgactaca tctgcacttc tacctgagtt tgaaacaagc caaagcactt
      241 tctttttctc attgctgaca agttgcggga gcaagacgtg gatatctttt tcaaaaatga
      301 ttggcttacc gacacgagac catttttgca gaatatcttc tactactaaa gaagaggcgc
      361 caaccccata taaaaaaaca gtctctgccg attccaaaca ctctacgact ttttggatcg
      421 tttcttcttc tagaatatct actgtttctt ttatcgctac ttgtgcattg ttaccaagtt
      481 tgtttttgat agagtaaaag gactcatttg cttctatatc actaaaacct tcatcgattc
      541 cagtctgtag ttctgttgaa atcgtgattt tgaagtccgt cagactttga aaaccgattt
      601 ttttggaaaa acgtacaact gtcggcgcac tcaacccgga agcttctgct aattcattag
      661 ctgtcattgt agggaccttc tcaatattac cgataatgta gttggccaac tttttttctg
      721 ctgaagaata atcttccatc atactttcaa tacgactaat tacgctcata agttcctcca
      781 cccttttaat gatagtctat gtaagtatac catatcagac ccttattcac ggtcgtatac
      841 tggataaaaa acaaaaaaac aatccgccca acagaatatc ttatcccatc aatcccacag
      901 attctattga gcatctgtgt ctttgaggaa tacctgttat ctattgttcg gattgctttc
      961 tatctactac ttttttacag gaaatttacc tattagtttt ctctgctgta atttttgact
     1021 tctgcttcca tagcatcaat aaagatattc aaatcttcta cagctgtttc tttacttcca
     1081 taacgacgta cgttcactgt tgcatcttcc aattccttgt ctccaacaac taattggtaa
     1141 gggatctttt gtgtttgaga ggcacgtatc ttatagccca ttttttcatt acgatcatca
     1201 acttctacac gcaacccctt catttgcaaa cgttctttga tttcataagc ataatcgcca
     1261 tgtgcatcaa cagaaacagg gatgattgtt gcttgtatag gtgccaacca tgttggaaaa
     1321 gcgcctttgt atacttctgt taaataagcg acaaaacgtt ccattgtaga aacaattccg
     1381 cggtggataa ctactggacg atgcgtattt tccccgtctt ctccaacata tgtcaaatca
     1441 aagcgctctg gcaataagaa gtctaattgt atagttgata atgtttcttc tgtaccaaga
     1501 gctgttttga cttgaacatc tagttttggt ccgtaaaagg ctgcctcgcc ttctgcttca
     1561 aagtaatcta gttctaattc atccatagcg gctttcagca ttgcttgggc tttttcccac
     1621 attgcgtcat catcaaaata tttatctgta ttgtttggat cgcgatagct caagcggaaa
     1681 cgataatctg taatattgaa gtcagcatat actgccacca tcaattcaag cgtacgttta
     1741 aattcatctt tgatttgatc aggacgaaca aatgtatgac catcattcaa cgtcatttct
     1801 cgtacacgtt gtaaacctga caaagcacct gatttttcat agcgatgcat cattcctaat
     1861 tcagcgatac gaattggtaa ttcacgataa gaatggatcg tgtttttgta gaccatcatg
     1921 tgatgagggc agttcattgg gcgaagaaca agcatttcgc catctcccat atccattggc
     1981 gggaacatgt cttcttgata atgatcccag tgtccagatg ttttgtacaa ctcaacatct
     2041 cccattattg gtgtatatac atgttggtaa cctagactaa cttctttgtc tacgatataa
     2101 cgttcgatcg tgcgtcggat tgttgcccct tttggaagcc agaatggcaa accagaacct
     2161 acttctgggg aaaccatgaa caagtcaagt tcttttccta gtttacgatg gtctctttct
     2221 tttgcttctt cacggagacg gatatattct ttcaaagctt tcttgtcaaa aaaggctgtc
     2281 ccgtatacac gttgcatcat ttgattgttt gagtttccac gccaataagc acctgccact
     2341 gataataatt taaatacttg tatacggcca gtggatggaa catgtggtcc acgacacaaa
     2401 tcgatgaatt cgccttgttg ataagcagta atcacttctt cttctggcaa ttcactaatc
     2461 agttctactt tgtacggatc acttgcaaaa atttctaatg cttcctgttt tgaaagaaca
     2521 cggcgttcga tcggcagatt ttctttgacg attttcatca tttctgcttc gattgccggc
     2581 aaatcttctg cagttacttg gttttgtcca ttatctgtat cgtagtaaaa ccctgaatcg
     2641 atagctggtc caacgccaaa gtggatattt ggatagaggc gacgcatggc ttgggccatc
     2701 aaatgagctg ctgaatgacg gactaagcca agtgcatctt catggtctgg tgtgatgatc
     2761 tcaatacttg catcttcctc gattggacga gtcaaatcaa cgagttttcc gttcacttta
     2821 ccagctaaag ctttttttgc taagcttttt gaaatgcttt cagcaattgc caatgttgtt
     2881 gttccagact caaattcttt gactgcacca tctggaaaag taatttttat cataatagtt
     2941 tcctccttga aatgttttga ttgatcagat catcaagctg aaaaacaaaa aaatcctagc
     3001 atctcttgaa aagagatact aggacgaaaa gttcgtggtt ccacctaatg ttcaaacgca
     3061 aatgcgttcc tcaagcgttg taacggtccg accgcccttg cttcaacaag ggtttcgaaa
     3121 gtggtcatcc tttggttttc tcctggaaaa gtttcagcct agcttttcct ctcttatcga
     3181 agactctcaa attcagttgg ctttctcatc aatcaattat gtgtttattt aaccactaat
     3241 aaatagaaaa atcaagtggg aaactaaact tattttttcc attccccatt tacaccgtat
     3301 gttccttttt tcatctcatt gatgatgaca tggatgtgtt cttttggtgc ctgcgtgttt
     3361 ttatgaacag cttccgtgat atctttcatc atattttcta attgttccgg actgcgacct
     3421 tctactaatt cgacatgtac gaatggcata atgctctctc ctctttctga tcgttctttt
     3481 ttcaactcga ttctatcata ctatgtttgt cttgggtact gaatcaggaa aaatggctgg
     3541 atcttttttt gctctcaagg cactcaaaat agagaccgta tccacgactt cttgcaaaac
     3601 agctccgaat aatgcgggga ttagtcccgt acttgcaatt aacatcaaaa aaatacaaat
     3661 aaagatgcca atcagtacag actgtttcgc tattctcatc gtatctcttg aaatcactac
     3721 tgcttttgct acacgcaaga gatcatcttt caagatgacc gcatctgcac tttcactagc
     3781 agctgtagaa ccatgagccc ccattgcaat accgatatct gcagcagcaa gtgctggtgc
     3841 gtcattcacg ccgtctccta ccatcgcaac aggtctttga tctttaggga tactttctaa
     3901 aatggtcagt ttatcttgag gcaagctacg ggcatgtaca gaatctaatt ggactttttc
     3961 tgcaatcgtt tgagcaatgg cagatccatc cccggtgatc atcatcaaat ttttcacatc
     4021 aagttttctt agctccttga ttgtctttgc tgcttctggg cgaatctgat ccgaaaatag
     4081 gatcgttcct gcaaaagatc gatcaattgt tacatagatt gctgtattgg acgtttctat
     4141 ttcttcagct gaaacaaaag aggcacgtcc aatctttact tcatgattat tgactactgc
     4201 ttggattcct tgaccggtca cttctttcag ttgactaaca ggatacagtt ggctgttttt
     4261 cacgttgttc actaacgaac gagctaagat atggctagat tcctgttctg cactagctgc
     4321 atacagaagt agctcttctt tttttattcc attgactggc tgaatcgcct ctacttccaa
     4381 tatccctttg gtaagcgttc ctgttttatc aaaagcaatc gtttttgtac gtgcgagctt
     4441 ttcaatcgtt gttccagttt ttacaacgat cccgttttta cttgaacggc tcattcctgc
     4501 aactaaagca atgggcgcag ctagaatcag tggacaaggt gatgccacta ccagtacctc
     4561 agcaaatcgt aaagggtctt ttgagaacca ccaagaaata ccaccgatca aataagcaat
     4621 caacgtgaaa gggactgcat aacgatcagc taaacggaca aatcgggcag gcttttcttt
     4681 tgattcttcg actagtttaa taatcatttg atactggctg tctgcggcac gtttttcagc
     4741 gatcatttgg atcgatccgt ctccatttaa agaccctgac ataatctgat ctccaggttt
     4801 cttggtttcc gggcgggact cacctgtcaa agaagattca tcgaccgaag attctccttt
     4861 ggtcactcga ccatctgcgg gtacaagttc gccaggcttg actagtaact ggtcatcaat
     4921 atttatttca tcgattacta tatcctgcaa ttttccttct ttgaatcgat gggcaattct
     4981 tggggtgtta tctaacaaag actgcagttc tttacccgct cgtttattcg cgtaatcctc
     5041 tagactatct ccgcccgtaa gcatgatcaa tatcatcaaa ctagcccagt attctttgac
     5101 ggctaatgtt gcaaggattg cggtaatcgc taatacatct acgccatatc ttcctgaacg
     5161 taacgtcttg atcatttcca aaaacatcaa gaaggcgaga atccctccag taaatgcgac
     5221 aatcgcaaat gcaagggccg gctggtgaaa aacaaattca cttaataaag ctatcccacc
     5281 aataagcaaa gtaagtaaaa attttttgaa atgtgtcatt gttttttctc ctcttttatg
     5341 tgacttagag aggttagtaa taaatttcca gctgcctctt tcatcttaag catagtcctt
     5401 gactagcact ttttaaaaca ataggcctta attgaaaaca actatcacta tcagcgtttc
     5461 caaaattcat cagttctaca aacaaaaata ttcagcaagg attacttttt cgattcatca
     5521 gttaaaaagt accttttttc gaaaaactat gctatacttt taattcgtta tttacggcgg
     5581 aaggagcaaa catgcgatga atgagaaaca actagaacaa acgcacgtgg atgaaacgat
     5641 cgaactaatc cagatcgaac aaaatatgct ggatacaaaa caacaagaat tgactcagga
     5701 aatgaatcat tcgacaaaag acacagctaa tcacaagatt cgagctggtt ccaatgaagc
     5761 tttttatgaa tctgctgttg aatatcgcca acatgagcag gaattgcttt tgaaatacca
     5821 tactcttgaa gcacagcaaa aacgcctgca aacgttgtct gtcatgaaag atagtcctta
     5881 ttttgcccgt atcgacttta aagaagaccg tgaaacagaa acattatatc taggtatcgc
     5941 ttctttaaga gatctggcag aagaaacaat cgttattgac tggcgcgcac caattgccaa
     6001 tctttattat gaaggtgaga ttggtcccgc attttatgaa actgaaacag atagaataga
     6061 tgtcgatctg cagttgaaac gacaatttaa aatccaagaa ggacgtattt tatctatggt
     6121 agatacgtcg gaagtaatca atgatgactt tttgctagag atattggatg aatcatcaag
     6181 caaccatatg aaaaacatcg tctctacgat acaaaaagca caaaatgcaa tcattcggga
     6241 cacaacaagt aaagtaatgc tgattgaagg aatcgccgga agtggaaaga catccgcttt
     6301 gcttcaacgt attgcttttc tgttgtatca taatcgcaag tggctcgagg ctgaaaacgt
     6361 tcttctattt tcacccaacc atttgttttc tgattacatc tcgactgtat tgccttcatt
     6421 aggtgaaagc ggcgtgccta cacagacatt caaaaattat ttggtgcagc tgctgccaga
     6481 gttttcttta ctagatgaac agcaacaaga aagtggtttt ttgctagggg aaaaagatcc
     6541 gatacaagtg atgaaaagcg gattgacctt agtcgatcag attgatcgct atatccaagg
     6601 catcacaaac tacggtccct tgttccgtga tatgaaaata aatggccgta cgatcctatc
     6661 aaaggaatcg attcgccaat ggtataaaga aacaaatgaa caattacctt tacatcatag
     6721 actttctcta ttacaaacga agctcctgaa aaaattaggc ggtctgcaaa aagacgaaac
     6781 acgtcagcaa tgggtcaaag atctggcgga agaacagctc caagaactat acgcaacaga
     6841 tccaaatctt gaatatacag aacaaaagga gcgttcatta cgtaaaaaac tggccaacca
     6901 aatcgtacgt aaaagattcc gtcgaatcca tcgagggatc atgaattatc aatttgtcaa
     6961 tataacgaaa caatatctcc actttctaca aactgtgcca ctttctcttc taacacgaca
     7021 taaaatcagc gagaccaggt ggcaggaaca tttgacaact gttcgtacac acttgcgtca
     7081 aagagaattg cgtcaagagg atgctgtact attcttttta ctgatgcgcc gaacatatcc
     7141 agtgacggtt acacacaaag ctcgctatat ctttattgat gaaatgcagg atttcgcacc
     7201 atcacaagtg gcattgctgc aagccttgtt cccaaaagca aatttgactt tctgtggaga
     7261 tctaaatcaa aatgtctttg gaaatgaaac gatcgttgga tcattggaag accttttcgt
     7321 ggaccgtgaa gtcactcgtt tccaattaac gacaagctat cgctccacaa aagaaatcac
     7381 tgattttgct aatcatttta ttgtgaaaga acatcaagta gaaacaacag caagaaaagg
     7441 agaccttcct ttgctcatcc aaggaacaac agatgatatc aaattgaatt ggttagcaga
     7501 taccattcga acgactcagg gaaaagcccg gtattggcgt acagcaatca tagctaaaac
     7561 acaagaagaa tgcgaagctc tttatgctca actgccagac gagttgaaac aacaagtcca
     7621 gctgatttta aatgagagtg actttatgaa gcgttccatt attttacttc cagccttttt
     7681 agcaaaaggt ctggagtttg accgagtttt cttgtgggat gtagatgcag aacatttttc
     7741 ttctcagcaa gacaaattga tcctttacac catgtgtaca cgtgcgatgc atgaattgac
     7801 cctttttacc tccaataatg aaagtccttt gttaaacaat atggatattt catcttatga
     7861 gaaaaaagta ctgtaagtaa aaagacaact gctatcatta atgttagcag ttgtcttttt
     7921 atttacaata ataattgcag aaaaagtaat tcaatcattt aaggtcattt attctatata
     7981 taaatccaaa tctgtttcag gatattttaa tttgacaagc gaaataatca ttttcagttc
     8041 ttccatgttt tctggatatt ctgggtcaaa taccttgcta tgacggccaa tcccgccatt
     8101 gtcgatcaat cggtaaattc gattccattc aatgaattgg tcaattgatt ggaaattgct
     8161 gattggatag ccgccacaat ccaaaatctc tgatacacga aatgctaggt atgcactatc
     8221 gatcacttta ttttttttcg tgtctatctt ctcatttctt tcgataagaa ttttttcagg
     8281 acgatgtttc aagctccact ctaatagtag aaagaaagcc tccatccgat actcttcttc
     8341 aaataaccga aaggctgaat cagaacgacg aaaaagcagc ggaatcagat aattttgtct
     8401 ataaatcagc attggtgcgc ggtccaccat acactctaaa taacggttca gataccctgt
     8461 accgacatag tttcctaatc gatagccttc aacgccgaat cttgcgtatt tttgcgctaa
     8521 ttttgaacaa tcagttacgt tcacagctaa ctcttctact ttgcggtaac cgcttaagat
     8581 acgttcaaaa ggttctaaag tgatttttgc ttccatattc ttccccgtcc atcttccaca
     8641 tacttcatat catttcacat gatacttatg ctctcatcat accacaggtt gaattagaaa
     8701 agaaaataaa tcacacaaaa aagcaagaca atctgttagt gagattgtct tgcttttcga
     8761 aaaagactga atacttttcg ttaaaactgg ttgcttagtc ttctaatgcc acattatgga
     8821 acacatgctg tacatcttct agatcttcca gtgcatcaat cattttttca aatgtttctt
     8881 gatcttctgg agatagtgtt acctcgtttt gagggatcat ttggatctca gcgattgaaa
     8941 aatcagtgat tcctttttct tttaatgctt cttgtatact atggaaatcc gctacttcac
     9001 cgtaaacgat gatttgtcct tcttcttcca ttacatcgcg aacatcgatg tctttttcca
     9061 ttaggtattc aaggatctcg tcagcgtctt ctcctgcaaa agcaaagatt gctgtattgt
     9121 caaacatgta agaaaccgct ccggaaacac ccatgttccc gccatttttc ccaaatgctg
     9181 cccgtacgtc tgctgctgtt cgattcacat tgttcgtcaa tgtatcaaca atcaccattg
     9241 agccgcttgg tccaaatcct tcatagcgca attcagagta tgtttcatct cctgcacctt
     9301 ttgctttttc gattgctcgg tcaataatat gttttggtac attataagtt ttagcgcgat
     9361 caatgacgaa acgtagtttt tggtttgcat gcggatcagg atcacctgat tttgctgccg
     9421 cgtagatttc aatcccaaat tttgcataaa tccggctatt attagcatct ttagcagttt
     9481 tctttgctac gatatttgcc catttacgtc ccatattttc acttcgcttt ctgatttgat
     9541 agtttcataa cgcaaaaacg tcctgttcca ttatacttgt gaatcacgat tctgtgaagc
     9601 tttttttctt tcttccttca ttcttctcat ttttagtcaa ttatctatac tcggattggg
     9661 atttgcccga aattctgttc cggaatattg cctcccaatt gatagatttg atcacttcta
     9721 actaacagtt ccgcttcttg tgcctctttt tttccgattc gggaataaat agaaatctct
     9781 tttttttctt ttaagaatct tcttccagca tcggtaaatc caagtagtcg aagataattt
     9841 ttattttgtt ggacaggaat ctcttgcttt ttgatattca gcaagacata acaagataat
     9901 cgttggatcc gtgtccatgt atatcttttt gtttttacct ttgacacaaa atcctcaaaa
     9961 ctatcagcag ttgcagctgc tgttttcatg cgcgattcta ttccttcggt catctgatag
    10021 atatctgtca gttccagcaa agtagaagat aacaaccgat aacgcaaata cggccaataa
    10081 gattcccaag tctgcagcga acaagttgaa agatcttctt ctgtctgagg cggcacaagt
    10141 ccagctattc tttttccttc ttctaacgca tggcgaatgg ctgttgcact ggctatcctc
    10201 tgatctgtga tgattttttc atgatagcct gcacttttac gtcggatcgg atgtaattcc
    10261 atagggtgga catacttggc attttccttt gcataactta atcccaagat atgatttggc
    10321 gaagaaaaat caagcgtaat atcgggatac agactgcgaa aaacctcagt cattttttgc
    10381 ggataactat acgtttgatc agacagttct tgaaatgcct cttctatcct atcttgattc
    10441 tcttggacaa agcgtccgaa actgctgtaa tcaaacgaag tctcttcttc tgttccaaaa
    10501 cacaaactgt cacagtccag tgtttgcaag atttttactg cacctttagc aaaatagtca
    10561 gctgactgaa ccgcccattc gacgggaagc tccactacta aatccacacc attttgtaaa
    10621 gcttcattcg cgcgatgcca tttatcgatg atagccggtt cgcctctttg caaaaaattc
    10681 ccgctcatta cagcaatgac gacctctgct cctgttattt ttcgtgccat ttccacatga
    10741 tagcgatgtc cattgtgaaa aggattgtac tctacgataa tcccgcagct tttcatgcgc
    10801 atcctccttc atagttatcg ataaaaaaga aatagagaca agtaaaaaaa ggcaaaaatc
    10861 ggaaaaaata cccgaactat ataaactatt ctcgcaaaca caatacttct gcgacgaaaa
    10921 gctgcactgc aagagcaatg cctatatcgt tcggatatat aatttatgtt gaagatttat
    10981 tcttttcttg ttaactcgag caggcaaaac cgctagtctt tgtatgctac aaagaaccag
    11041 cgctttgatt tctctgatgc tgcttcgtct gtaaaatcag aaaatgcttc gactttcgaa
    11101 aagccgctgt tttccagcat tcttaaataa ctttccaatg gataggtacg ctcttgatgt
    11161 agttcatctt cacgaataaa tcgaccagac ccttcttgtt cttcaacgaa aaaggtaaga
    11221 aaatgttcga tgctgtgttt ctcgcttcct ggataactat cccacaaaaa agcaaaatta
    11281 tccccttgat aatgatagct atattctgga aaaacctcat ctacttgata ggtggagtgt
    11341 acgtcaaata aaaatcggcc attttcttct aacgcttgat agacttcatc aaacacttgc
    11401 tgtacctctt gtctattttt catatagcac agcgaatcag aaaaacaggt aattgcctga
    11461 tattgtccga tttctgataa atcgagcata ttgccttcca caaattggat cgatgcgtct
    11521 tcttcatatg ctcgctggct ggcaatcatc aacatctctt ctgaaaggtc taaccctgtc
    11581 actttatatc catcttttgc aaaggcaacc gctaatgctc cagtaccgca tgccagttcc
    11641 aaaatatttt ttgtgtcttc ttgtaaatga cgtttcgaaa aagctaacca ttgctgataa
    11701 agtgtttcgt ccatcacttc atcgtaaatg aaagcaaatg tttcataagc catatgttaa
    11761 tccaaccatt cactaagatc gactagcgga gcatctgacc atagtttttc cagattatag
    11821 aaatttcgtt ctggcgcatg gaaaacatgt acgatcacat ctccaagatc gatcaatacc
    11881 cattttccgc cttcttttcc ttcaatacgt tttacttcat atttgttttc ttcttcttta
    11941 tcaatgattt cttctgtaat tgcgttgatt tgacgttcgc tatttgctga acagatcatg
    12001 aaataatctg ccaacaagga aacatttcga acgtctaaag cgataatgtc ttctgcccgt
    12061 tttgaatctg ctgcttcaat cgcaattttt aattgttctt tactatctat gatgatttcc
    12121 tcctaatatg agcagcttat ttttttgcta cccagtaatt atatgtttcg attgtttttg
    12181 gataaatctg ttgttcttgc gcaagcaaat gggccaatgt atgttttgtt tcaaatgcta
    12241 cagcttcgtc tagatcagcc catgcaatgg ctcgtgcatc ttgtacccca ggaaaatctc
    12301 gacctggttc aatatagtct gctacataaa tgattttgtc aaggagactc atcttagcag
    12361 ccccagtcgt atgcacccgg attgcatgaa gaatctctgc atcggtgatt cctaattccc
    12421 gctctacaaa cgaagcgcca acaagaccat gccagattgc attattataa ttcaataact
    12481 ccggatcaaa accgtctctt acgattacca ttttgaactc ctcatctggt cgttcttttg
    12541 cataatcatg tgtcaatgca gcaatactag ctttttcagg agaagcaccg tattttttgg
    12601 caagagccac ggctgtttct tctactccaa gcacatgctt gaagcgttgt tcgctcattc
    12661 tcatctgtat tttttgcatc aggttttctc tagaataagc agtatattca ttactatagt
    12721 ccatattcat acaacccttc attttgaata taatcaatta ctttatctgg aacaaggtag
    12781 cggatagaac agccttcttt gatcttttga cgtattttcg tggaactgat gtcgatctca
    12841 ggtacatcta cccaaattac aggatatgga gtatccgttg tgtatcctgg tcgacgtata
    12901 cccacaaagt tgaccataga agtcagctca tcgattttat accattttgg caaatattct
    12961 accatatccc caccgataat aaagtaataa tctgtatccg gattgttttg cgtcaattcc
    13021 ttcatagtat cataggtgta acttttccca ccacgagcta attcaatcgt ttcaatctgc
    13081 aaaaaaggat tgtcttccac cgctaattcc aacatgttga gtcgatgttt tgcatcaatc
    13141 gtctgtttct catcaacatg aggtggctga taagaaggca tcaaaaatac gcggtcaaga
    13201 cctaaattcc ggccagcttg ttcagccatc actaaatgtg caaggtgaac gggattgaat
    13261 gttccgccca agatccctac ttgctttttc ttctctaaga attgaggcat ttcttcagga
    13321 aataattgtg aacgaacaaa tgtcttagct tgcgtattca tcatggtcac tccttatatt
    13381 gcttttactt cttttgatag cctttgatac ttttcttttg tggaaggttt gtataataca
    13441 ataacacgtc caatgatttg gacgatttcg caatctattt ctttttctaa tacttgagct
    13501 gcttcttctg caatctcatc tgtattttga agtaacgata ctttgatcag ttctcttttt
    13561 tctaaagctt cttcaatttg aacgatcatt gctgaattga tcccaccttt tccgatttga
    13621 aaaatcggct gaagatgatg cgctttgcta cgtaaaaaac gcttttgttt tcctcttaaa
    13681 ctcataattt tccttcttcc ttaaatcaat gctttgcgtt tcaatacatc tacacccttt
    13741 ggcgcccagc ccgcaattac tactggcgct gttacagtga tccagcctag accggcaaaa
    13801 acgatgtctg ttttttcttt cacagaaaac tcaaaacgaa caagctccgg aaatgcctcg
    13861 acttcatcag gacgaggagg ctgtaataat ccacctacat gtttttgata aaattcgtct
    13921 gctttttcca gtttcgttcg atgaatattt acatcatttg acacataagc aataaatgaa
    13981 gcacgattgc cgctgataaa atcaaatctt gcaagtccgc ccaagaaaag tgtttgccct
    14041 tcattcagct gataaacttt aggtttgatt tcttttaacg gtgcaatgat ccgcaaatct
    14101 tttttaccta agtagtgcgc catttgatgg cgatggataa ttcccggtgt atcaatcaaa
    14161 aaatgcccgt catctaaagg aatctcgatt ttatccaagg tcgttcctgg aaactgcgaa
    14221 gttgtgatta catcttgtac tcccgctgta tttttgatga tttgattgat aagcgtcgat
    14281 tttccgacat ttgttactcc gacgatatag acatcccgtc catcgcggta ttcttcgatt
    14341 ttttctaata gttccgtcat ctcatgagat ttcttagcac tagttaataa tacatctact
    14401 ggacgaattc ctgcttcgta agcacgttcc ttcatccatt gtgttaattt agtttttttc
    14461 aatgatttag gtaatatatc cactttgttc ccaactaaaa gtactggatt gtcaccgaca
    14521 aaacggtgta gtcctgggat tagtgaacca ttgaagtcaa aaatatccac cacattgacg
    14581 atcaatgcat ctttttgccc taattcattc agtaagcgca aaaagtcgtc atccgtcaat
    14641 gagacatctt gggtctcatt ataatggcgt aatctgaaac aacgctgaca atagacttcc
    14701 ccggaagcta aacctttttc caaagccgtt tgcggcgtgt atcctaattc accgggtttg
    14761 tttgtttgta taacggcacc acagccgata cattgtaatt cttctgtcat tcgattccac
    14821 ctcgccagcc catatcaggg tgttttgcta atagataggc cataattctt ttttcccaaa
    14881 aacgattaaa ttgtgtcttc caactatctg ttgcgataat gggttttaca aggatgctac
    14941 gtatccctgc acgattggct ccacgtatat cagtcataat ctgatcgcct accattacta
    15001 tctcatcatc tgctaaatcc aattgtttct ttgctatgtt gatccctctt gctaaaggtt
    15061 tcattgctcg tgcaacatac aaaagatcaa atttttcaat cgctcgcgcg acacgttttg
    15121 aattattgtt tgaaacaaca acaacaggga ttcctgcatt tttcatttct aaaatccaag
    15181 taagcaactc ttctgttcca tctggattat tccatgcaat caacgtattg tctaaatcgg
    15241 tcaatactgc tttgatgcct agattcttta attgggcagg tgtaatctga tagatggctt
    15301 ctaccatcca tgttggtttg taatttgaaa acatgctgtc tcctcatctg caaaaaatga
    15361 aggagcacat aaagtactcc tcaaccccgt attatactgc atatcagcaa aaaaaactac
    15421 ctctatactc gttcgtcttt cagaaaaagc atttctttac aaaatgacca aaaataaaga
    15481 ttgatcacag aaaaatttag aaacaaaaaa caactaatat tggcggaacc tcttgtatcg
    15541 tttttccagt aatttatgct gatctaatcg aacaagttcc gtcaatcttt caatcaacgc
    15601 ttttttcata atgtgtgcga ctttatatcg tggcaactgc tcgccgttga acgtttcagg
    15661 aataactcga tcaacgattc ctaactcgcg tagttctggc gcagtaattt tcatcaactc
    15721 agctgcttct gcggcgcgac taccgtcttt ccacaaaatc gacgcaaatc cttctggaga
    15781 aagcacagca taaatcgttt cttccagcat ccagacttca tccgctaaac ctagagcaag
    15841 ggcaccgcca cttccacctt caccaatgat gatggcgatg atcggtacct ttaaatcaga
    15901 catttcgagc agatttctgg caatagcctc tccttcgccg cgctcttctg ctcctacacc
    15961 gcaataagca cctgctgtat tgatcaaggt aatcaccggt ctattgaact tttccgcttg
    16021 tttcatcagt cgtaaagctt ttcgatagcc ttcaggatgg ggagaaccaa aatttctgcg
    16081 gatattttcc tgcagatccc gtcctttttg aataccgatc actgttaccg gtttgccatc
    16141 taatcgagcg attccaccaa caatcgcttc gtcatctgca aataaacgat caccatggca
    16201 ttcctcgaaa gaatcgaaga tctcttcaaa aaaatccaag cttgtccatc gttctttagc
    16261 tcttgctaac tgaacgacat catgtgctgt cttatccacg atctatccat cctttctgtg
    16321 tatgcaattc aagaagcgta tggattttct gtttcaattc catgcgggga acaatctgat
    16381 cgacaaaacc atgagataat aagaattcag ccttttggaa atcttctgga agttcctgct
    16441 tgatcgtctg ttcgatgacg cgtcttccag caaaaccgat caagctttgg ggttctgcta
    16501 gaataatatc gccttccatt gcaaaactgg cagttactcc tccggtcgtc gggtccgtta
    16561 aaacagttag ataaaaaaga ccttcattgc tatgccgttt gacagcagca gagatttttg
    16621 ccatctgcat tagagaaaag attccttctt gcatccgtgc gccacctgaa gctgtgaaca
    16681 agacgactgg aagactttcc tttgttgctc gttcgaataa tctggtgatc ttttctccga
    16741 caatcgttcc catacttccc atgataaaat taggatccat cactccaata atgaagggaa
    16801 tcccctgaat ggttgcttta ccagtgagga ctgcttcatg aagtcctgtt ttatcttgca
    16861 tttttttaat tttttccgta tattcgggaa atgctagcgg atctttggtt acaagatccg
    16921 tatcccattc ttcaaaactt ttttcatcaa taatcaaagc tagacgctgc catgcaccga
    16981 ttcgaaaagc gtatccgcaa tgagggcata ctttttcttc accgatgtct ttcgtataaa
    17041 ggatatgttt gcaatttgga cactttgccc acatgttatc aggaacagat ggcgagttag
    17101 caggtttgct ctctctatta ggattgatcc gaatatattt ctttttctta aacagagcca
    17161 tctatgcgcc tcctatctcc gtttctggtg tccaattcgg taaaaattct tcttgtaaaa
    17221 atgctgtgct gtaatcacct gctatgactg caggatgact aatcaaatcc atctgaaatt
    17281 ctgcgttcgt gatgattcct tctgtgataa attcacttaa ggctcgctgc atcttcatta
    17341 atgcttcaaa acgcgtattt ccatggacga ttatttttgc aatcattgaa tcataatatg
    17401 gcgggatcgt atatcccggg taaactgcac tgtctactcg cagtcccatc ccacctgctg
    17461 gaagcagtaa gttttggatc ttccctggag aaggagcaaa atggaaagca ggattttctg
    17521 cattaatccg gcattcgatt gcgtgtcctt ggaatactac ttcttcttga cacacattca
    17581 aaggctcacc ggctgctatc tccacctgtt tttttactaa atcgattcct gtgaccattt
    17641 ctgttactgg atgttccact tggattcgag tattcatttc catgaaataa aaatcgcctt
    17701 cttcatccat cagaaactcg atcgttccgg cattttcata attgactgct ttagctgctc
    17761 gaacagcagc gtctcctagt gcttctcttt ttgtttgtga gatcactaca gaaggagatt
    17821 cttccagtac tttttgattg ttgcgttgta aagaacaatc tcgctcgcct aaatgaatga
    17881 catgaccaaa tcgatcacct aagatctgaa cttctatatg gcgagcagga taaatgattt
    17941 tttccaaata catatcatca tttccaaatg ccgctgctgc ttcttgctga gctgaagaaa
    18001 aatgctgtgg gagctcttct tttgattgaa ctttccgaat gcctttcccg ccaccacctg
    18061 cagctgcttt cagcatcact ggataaccaa ttctgtctgc tatttccaat gcttcttcta
    18121 ctgtagataa tacaccagta cttcctggaa tgactgggac atttgcttct tgcatcaatt
    18181 gacgcgcatt gatcttattt cccattgcat cgatcgttgc acttttagga ccaataaatg
    18241 tgatattgca ctcttcacac atggaggcaa atcggctatt ttcagataaa aaaccaaatc
    18301 ctggatgaat tgcttcggct tttgtcacga ttgcagcgct cagtacttcc tgaacattca
    18361 gatatgaatc aacggctttt gccgggccaa tgcagattgc ttcatctgct aactgtgtat
    18421 gaagtgcttc ggcatctgct tctgaatata tcgctacggt ttggatccct agttcgcggc
    18481 atgcacgaat gatacgtaca gcgatttcac cgcggttagc tatcaatact ttagaaaaca
    18541 tatttcacct atccaatcat aaaggttaat tccgcttcgg ctactttttt accgtctact
    18601 ttagcgattc ctttgcctat tccggctgaa gctttcactt tcaatatctc aacttcaagg
    18661 tataaggtat ctcccggtgt aactttctta cggaattttg ctttgtccaa tccgccgaaa
    18721 taagccgtct ttcccttgaa ttgttcaaga gaaagtaatg caactgctcc tgcttgagcc
    18781 attgcttcaa caatcaatac ccctggcata acaggttctt ctggaaaatg tccttgaaaa
    18841 aagggctcat tgatcgttac atttttctta gcaacgatcc gttctccttc aaccatttct
    18901 tccactcgat caatcaaaag cattggataa cgatgtggaa tgatttcttt tatttcttgt
    18961 atgttcattg ttttccctct ttcctgcttt gcttattttt gctgaatcct aaagagtggc
    19021 tgtccatatt cgacaactga ctcgttttct acaaggattt ctgtgatcac tccgtcggcc
    19081 tcactagtta tctcattcat cagtttcatc gcttcaataa tgcagataac ttctcctttg
    19141 gcaacttgat cacctacttt tttgaaagct ggtttgtcgg gcgctggttg cagataaacg
    19201 attcccacga ttggcgaaac aacctcgatt ccatcaaata ctgccgattc tttttctttc
    19261 attatttctg gagcagttgt tgcttgttca ggtatgacat tgactggctc cgaaactggc
    19321 aacgtttcgt ttgtttgaac catattcatg cctcgacttg ttttattttt attcatatac
    19381 aattcaaatt gaccttcacg caaatcgaat tccgttagcg aggattgatc aaattgactg
    19441 actaattctc tgatttcatt gatatccaaa attattcctc ccagcgtttc atgcacagaa
    19501 ccgcattatg tccgccaaac cctaatgaat tactcattgc ataagtaaaa cttgcaggtt
    19561 tgctttcgtt tttgacaact gtcaagtcaa tctcaggatc ttgtttttct acattgatcg
    19621 taggtgggat aaattgatgt tctaatgcca gcaaagttgc caaggcttcg atcccgccgg
    19681 ctgctcccaa taaatgtcca gtcatcgatt tcgtgctgct gacgtaagta tctctgaaat
    19741 tttcacctaa tgcgatttgg attgcttttg cttctgcctg gtcattggct ggtgtacttg
    19801 ttccgtgagc attgatatat ccgacttgtt ccggctgtac accagcttct tccatcgcaa
    19861 gcttcattgc tttagatgca ccagaaccat ctggagtagg agctgtcata tggtaagcgt
    19921 cacagtttgc accgtatcca acgatttctc catagatttt ggcaccgcga gcttgtgcat
    19981 gttctaaaga ttcaagaact agaacgcctg ctccttcacc catcacaaaa ccattccgat
    20041 tttcgtcaaa cggaatcgaa ccacgattag gatcagttga ttctgagagt gctgttaaag
    20101 ctgcaaatcc agcaatcccc atctcacaaa tcgttgcttc tgttcctcca gcaagaatga
    20161 catcagaata gccatgtttg atgttacgga atgcttcccc aattgaattc gttccgctag
    20221 cacaagctgt tacgatagct gtacaaatac cttttgcacc tacacgcaaa gcaatatttc
    20281 cagctgccat gttggcaatc gccattggta cgaacattgg agaaactcgt tttgcacctt
    20341 tttcatgtag acgaataact tggttttcga ttgttggaag accaccgatc ccagatccga
    20401 tgatgacgcc aaaccggtct tgattcattt tttctttatc taaaccgctc atctccattg
    20461 cttctaaagc agcgtagatt ccatagatcg aaaacatatc cattcgtttg ctatcttttt
    20521 taataaaata tttatcgtaa gggaaatctt tgacttcacc cgctaaggta atccctgttt
    20581 ctgaagcatc aaatttagtg atcggtgcga tgccattcgt cccctctttt aagctattca
    20641 aaaagctgtc tgcgtcattt ccaataggtg aagtgactcc atagccagta attaccactc
    20701 gattcatttg tttgctctcc ttttcttatc cgtgcatgac caatccgcca tcgacattga
    20761 tgacttgtcc ggtgatatat ggattttgtg ctaagaaaac tgctgtttgt gcaatatctt
    20821 ctacttgtcc gaaacgctgc atcggaattt gcttttccgc ttgttccttg actttgtctg
    20881 ctaaaacttc cgtcatatca gttgtgatga atcctggcgc aatggcatta catgtaatgc
    20941 ctctagttgc agcttctcgt gccacagatt ttgttaatcc tactacgcca gctttactag
    21001 ctgcataatt tgcttgacca atatttccaa tcaacccaga tacactagag agattaatga
    21061 tggccccttc tcgctgcttc atcatttttt tcaatacgtg ctgcgtcata ttgaaggttc
    21121 caatcaaatt gatatccaga cattttttga aatcttctgc atccattcgc atcacaagtt
    21181 tgtcatttgt gattccggca ttgtttacta acacatggat cgatccaagc ttttcttctg
    21241 cttctttgat catttgtccc gctttctcat aatcagagat gtcaccggaa actcctacgc
    21301 attttacacc gaatgcttcg atttcttcta tcttttcttt tggaatctct ccgcgaccat
    21361 tcaaaatgat atttgctcca gcttttgcaa aagctaaggc catcgctttg ccgattcctc
    21421 ttgtactacc tgtgataaat acattttttc cttttacatc cataacgttc ctccttatag
    21481 tattgctatt gtttcatcaa atgtctgctt attctcaaca cgcagaactg gtattgtttt
    21541 atcgattttt ttcataaatc cgcttaatac ttttccgggg ccgacttcga tgacttgttc
    21601 gatgccgatt gtttttaact tatggatact ctcatagaaa cgaacaggtt gcatgacttg
    21661 ctgttcaagt agtcccgcga ttgtttcttt cttcattatt tccgttgttg tgttgcttat
    21721 gataggaaaa gaaggttcag agaattgaat ctgacttagg tccttcgcca gtttttttgc
    21781 tgccggttct aaaatagctg tatgaaaagg cccgctgaca tttagaggga tcattctttt
    21841 gaaccctttt tcttttaata gtaaaacagc ttcgtctact gctttttctt cgccaccaat
    21901 cacgatctgc tgaggtgtat tataatttgc aggagatacg atgccgtact tgctagcttc
    21961 atgacagctt tcctcgatcg tttcaatcgg cgcattcatc actgcaacca ttttcccgct
    22021 tccagctggg gcagcttctg tcatataagc gcctcttttt gctactaacg caactgcttc
    22081 agtgaaggat agcgcattac tagctactaa tgccgaatat tctcctaaac tcagtcctaa
    22141 agctgcatct gggatgattc catgctcctt caataagcga taatacgcga tgctgactgt
    22201 tagaatagct ggctgtgtat attgcgtctg atccaaacgt tcattttctg taaagcaaag
    22261 ttcagccata tcgtagccga gaatctcact tgcttcatca aatgtctgct ttacaacggc
    22321 ttcttcatat aaatcttttc ccataccttg atattgtgca ccttggccac taaataagaa
    22381 tgctgttttc atgtttttct ccaatatatc cagaatatat gtttgattct tcctattcaa
    22441 aaaggccgag acattcctat ttcaggaaat gtcccagccc ccaacctaat tttaatgatt
    22501 atgcagtttg tttttctacg taagtcacta aatcttgaac cgttttgatt ccgtcttctg
    22561 tttcgatttt cacgtcaaat tcatcttcaa tttcattgat gatttggaat aaatccaagc
    22621 tgtcagcttc taagtcttct tggatgtcag tagtcaattt gatttcttca gtttcttttc
    22681 ctaattcttc tgcgatgatt tcttgaattt tttcgaatac cataataata tattcctccg
    22741 atattgtttg ctttttattt ttataattta acagtctaca actccatgag aatacttccc
    22801 catgttaaac ctccgccata gcctgtaaat attactttct gctgttttcc taaggtgatc
    22861 tttccactag aaactgcttc atccaataag atagggatgc ttgcagcaga tgtattccca
    22921 tatttatcca tattggtcag gaacttttct tgcggtatct ttacttttcg ggcaattttc
    22981 tcaataattc tgaggtttgc ctgatgcaaa agcagataat caactggttt gtctttcatg
    23041 acttcttgga tattgtttgc aacgtctcgt acggcaaaat caaaaatatc tctgccagtc
    23101 atttgcaaat aatacgaacc ttctgaatct tctgaataga acggattttt attttgctgg
    23161 tagccagcgg tcaatgagct acttctggta ccgtcagctg ctaatttttc tttcagcaat
    23221 tttttctgtg gattcgcttc taagagaatc ccacctgctc catcgccaaa caacacagca
    23281 gtcgtacgat cagaccaatc gattagctta gatagtgttt caccaccaat caccagtccc
    23341 ttagttgagc cgcagcggat cattttttca gccatgctta acgcataaac aaatcctgag
    23401 cacgcagcac tgatatcaaa agcgatagca tgagttgctc ccaatttccc ttgtacttgt
    23461 gctgctacag atggtgtcgt gtaatcgggc gacattgttg ctactaaaat aaaatccaaa
    23521 tctttagggt ttagattttt ctgttttatc aatttttccg ccactaaaat gcataaatca
    23581 gaagtctcct gatttgttac acatcttcgt tcacgaatac ccgtccttga cgaaatccat
    23641 tcatcagagg tatccatcat cttggaaaga tcattattag taataatctt ttcaggaagg
    23701 acgctggctg ttgcagtaat cattccaaat tgttccatta aatatcctca cttatagtct
    23761 tgcaagaagt cgtgcaagtt tttcagtgca tccagcaagg catgctgttc ctccgtagcc
    23821 attccgtcta agatgttctt gaccatttca cgatgaaaat gctgatggac ccgaaatagt
    23881 aactttcctt ttttcgttag tcccaacttt acaacgcggc ggtcatcttc actgcggata
    23941 cgttcgacat agcctttttt tactaacttg ttaatagcag tggtcaaggt cccaacagta
    24001 attgatagtt ctttggctac ttctgaagtt gtttttttct tgtacatccc gattgcctcg
    24061 atcgtgtgca tctctgtgat tgacagatct ttgaattgag attttttcag ctcagactct
    24121 tcgattgtca aaatatcatt gaagacacta acaagataat catttactgt ttttaaatct
    24181 ggttccatgt tccaccgtcc actaattagt ttgattatca aatcttttga tgttcaaact
    24241 atatcttaa
//
LOCUS       DAYYME010000030        23970 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000030 DAYYME010000000
VERSION     DAYYME010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.030"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            40..672
                     /locus_tag="ACHBFW_001000"
     CDS             40..672
                     /locus_tag="ACHBFW_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317325.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(806..931)
                     /locus_tag="ACHBFW_001001"
     CDS             complement(806..931)
                     /locus_tag="ACHBFW_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFM9317326.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1065..1958)
                     /locus_tag="ACHBFW_001002"
     CDS             complement(1065..1958)
                     /locus_tag="ACHBFW_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFM9317327.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2140..3066)
                     /locus_tag="ACHBFW_001003"
     CDS             complement(2140..3066)
                     /locus_tag="ACHBFW_001003"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFM9317328.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3151..3912)
                     /gene="pflA"
                     /locus_tag="ACHBFW_001004"
     CDS             complement(3151..3912)
                     /gene="pflA"
                     /locus_tag="ACHBFW_001004"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFM9317329.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4156..6387)
                     /gene="pflB"
                     /locus_tag="ACHBFW_001005"
     CDS             complement(4156..6387)
                     /gene="pflB"
                     /locus_tag="ACHBFW_001005"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFM9317330.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6664..9114)
                     /gene="parC"
                     /locus_tag="ACHBFW_001006"
     CDS             complement(6664..9114)
                     /gene="parC"
                     /locus_tag="ACHBFW_001006"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFM9317331.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9126..11171)
                     /gene="parE"
                     /locus_tag="ACHBFW_001007"
     CDS             complement(9126..11171)
                     /gene="parE"
                     /locus_tag="ACHBFW_001007"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFM9317332.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11337..11771)
                     /locus_tag="ACHBFW_001008"
     CDS             complement(11337..11771)
                     /locus_tag="ACHBFW_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFM9317333.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11898..12536
                     /gene="plsY"
                     /locus_tag="ACHBFW_001009"
     CDS             11898..12536
                     /gene="plsY"
                     /locus_tag="ACHBFW_001009"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFM9317334.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12668..13543)
                     /locus_tag="ACHBFW_001010"
     CDS             complement(12668..13543)
                     /locus_tag="ACHBFW_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM9317335.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13625..14416)
                     /gene="codY"
                     /locus_tag="ACHBFW_001011"
     CDS             complement(13625..14416)
                     /gene="codY"
                     /locus_tag="ACHBFW_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFM9317336.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14434..15834)
                     /gene="hslU"
                     /locus_tag="ACHBFW_001012"
     CDS             complement(14434..15834)
                     /gene="hslU"
                     /locus_tag="ACHBFW_001012"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFM9317337.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15848..16396)
                     /gene="hslV"
                     /locus_tag="ACHBFW_001013"
     CDS             complement(15848..16396)
                     /gene="hslV"
                     /locus_tag="ACHBFW_001013"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFM9317338.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16433..17338)
                     /gene="xerC"
                     /locus_tag="ACHBFW_001014"
     CDS             complement(16433..17338)
                     /gene="xerC"
                     /locus_tag="ACHBFW_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFM9317339.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17536..18912)
                     /gene="trmFO"
                     /locus_tag="ACHBFW_001015"
     CDS             complement(17536..18912)
                     /gene="trmFO"
                     /locus_tag="ACHBFW_001015"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFM9317340.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18881..20959)
                     /gene="topA"
                     /locus_tag="ACHBFW_001016"
     CDS             complement(18881..20959)
                     /gene="topA"
                     /locus_tag="ACHBFW_001016"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFM9317341.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21081..21938)
                     /gene="dprA"
                     /locus_tag="ACHBFW_001017"
     CDS             complement(21081..21938)
                     /gene="dprA"
                     /locus_tag="ACHBFW_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFM9317342.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21994..22761)
                     /locus_tag="ACHBFW_001018"
     CDS             complement(21994..22761)
                     /locus_tag="ACHBFW_001018"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFM9317343.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22754..23614)
                     /gene="ylqF"
                     /locus_tag="ACHBFW_001019"
     CDS             complement(22754..23614)
                     /gene="ylqF"
                     /locus_tag="ACHBFW_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFM9317344.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         6256 a         5149 c         4117 g         8448 t
ORIGIN      
        1 ttatacttta tatacttatc aatttaattt ggagatatta tggaaattca acgtagaacc
       61 actaattttt tacatttagg ctcttttttt gcaactttga cgatcttatt ttttctatgt
      121 tattcatacc ttaagacctc acatgcgcct tctagtgcat taggctggtt gcttttggat
      181 cattctgata tcacagaagc cgaagaaaat ccgttttttt tgattttgtc ttctctttgc
      241 ggcttactgt tttctgttta tttcggtgcc tatttttttc atcgacatcc tggtacgtta
      301 aacgaagctt caagcgtatt gattgcattt aaaacgtttt cttttttagc aattcttttt
      361 tattcgttta atcacaatac gcttttcttc tttatcatga atggtttagt cgtgatttta
      421 tccattttta cttattttat tttttcactc atttttagcc aattgaaatg tccgctcttc
      481 tttcgattag ttcctgtcac ctgttttatt tacagcttgt tttccttttt tgtccttgct
      541 ttaatgccac tgaccaaccc ttcgacgatc caagtcgcag gaaatctgtt ggatatgctc
      601 ttcatcgtca gtctaggtgt atttatgtgg caaagaaaaa acaaacaaaa taaaaagcat
      661 tttgaagcct gactattacg acttcataaa tgctttttta gttttaaaca cttcaatcta
      721 aaagaataac ccaagattgg ataaattatt aatgactcgc cattcgtttc ttagtgattc
      781 tggattatca aatggacttc ctaatctaat cagctagttc ttcccataaa atgccatact
      841 tgactgaatc ataatattga ttatttaaaa atctaacttt tcttatcact acttcctttg
      901 tcattccaca tttctcacca tttttttgca ttcctttgtt tccagaccat gtagtgaatc
      961 taataggtgg tagataggca aaatactcaa ataattcttt taaccataaa ggaagagaaa
     1021 ggctcctcct gcaagagtca gtataatttt tgcttactgc tctcttaggt tttactcact
     1081 aaaactgttc catattcttt gatataagaa aatgcatgcg gtataaattt cttgctcagc
     1141 tcagaaaaga tttgtcgttt aagaaaggcc atttcctcca ttgtcaatga aggatccaat
     1201 gttggtaatt caatctcaaa attgtttttt tcgacaatag gtaattctaa tgtgaatgat
     1261 tcttcttcct ttatagtatt cgtcacaagt agagactgat ggaaaatact aggccggcga
     1321 tttttatatt tattcttacg tatcctgtca acgataactt ctatttgatc aaaaaagtac
     1381 gattcattta cgtctccaat aagttcggct aattctttga atgccttcgt tacaggaagc
     1441 tgattcttct gtacaaaaaa gtaattagcc aaagtaagcg ggaagtgttc tatagaggaa
     1501 agtgtgataa aacggtactt ctcatttcct gcagttgtcc tcagcaattc tgtctctcgg
     1561 ctgtttctta ctccataaaa atacgcttct ttccgttcat cttcgaaaca atacgcccaa
     1621 ataacttctc ccatcgctaa tttttgttca tcaacagaga gtctcttcaa ttgttcagct
     1681 atggtttcag cagccgaagt ggcttgttgc aagtaatctt taggatcaaa tattggaaag
     1741 ttcaaatgat atcttcgttc ttccctcttg attaatcctg catcgatcaa taaatcaaga
     1801 aaatgggaaa atttattttg cgaaaattct tttttcaatt ctcttagaat cgtttcttgt
     1861 tctcgatgtt catatagata atcggtcaat gaagaaaaaa gcggatcatc ggtcagtttt
     1921 tgataaaagt tttccttatt ttttgaataa tagttcatat atgttctctt ttctatccgt
     1981 tttattttac ttaaaaggct tttacgtaaa aaggaggaag aacaagagtc ataattgact
     2041 gttattctcc tccatttctt cggcctttag tatgttctat gtttcttttt atatcaatcg
     2101 cttatccatt cattttgaat gcttcatact accatttgat tagctaaata cagccgtcaa
     2161 ttgaggtacg acttgttttt tacgtgaaac gacaccttta agcaaagcac ggttgttggt
     2221 caaagttgtt tggaaagctt cttctacttt ttcttttcct ttaccagcaa caagtaattc
     2281 tgaatcactg tctaagatgt ttgtgacaac taatacgaac agatcgtagc catttgcttt
     2341 catttcatct tccattgctg cttctagctc tgcttgtcga tccatcactt cattaaggtc
     2401 cactgtattg acttgagcga tacgaacgtt ggaactgccc attgggaaac tttttgcatc
     2461 caaatcaaga agaaccgcag ctgatttatc acttagattt gttcctgctt tcaacatttc
     2521 aagtccataa gtgtttagat cgacttcagc gattgctgca agttcttttg cagctgcttc
     2581 atcttcaggc gtacatgttg gtgatttgaa caacaatgta tctgaaatga tagctgagag
     2641 catgattcct gcgatttctt taggaattgt tacttggttt tctttatata gttttaaaat
     2701 aatcgtactt gtacatccaa ctggttctgc acgatagtac aatggattag ctgtttcaaa
     2761 gtttgcaata cgatggtggt ctactacagc taaaatattt acttcgctga tatctgaaat
     2821 actttgttgg aattcattat gatccacaag catgacatct gcattttctg ctttagctga
     2881 ctctactact cgtggtgcag atagctgaaa gtggtctaat gcgtattgtg tttcttcatt
     2941 aggtgtacct agagcaactg cttctgtttc tttacctaat tcattttgca agtgtgaaaa
     3001 cgcaatggct gccccgattg catctgtatc tggattttgg tgaccaaaaa ctaaaatttt
     3061 tgacatgtca ttcgctcctt tgtgatttct cctatttaat aatagcaaaa aaacggaaag
     3121 aagcaatact tatcttcttt ccgatgcgac ctatcgcgtt aaatattttg tatactcttc
     3181 tgtatgaagc aatcgtttag cattttcgac acgttctttt gtaggtggtt cgatcccttc
     3241 taatggatat ttcaaaccaa gtgtttccca cttgtatttt cccatcgtat gataaggaag
     3301 aatctctact ctgtctacat ttgacaaggt ttgaataaag ttgtctagtc ggatcaaata
     3361 ttcatcataa tcacttcgct caggaactag tacatggcga atccaaaccg gtttgttgat
     3421 ttttgacaaa tattgagcca tttctaaaat attttcattg tcccaatgcg ttagtttttt
     3481 atgctcttca ttatcgatgt gtttgatgtc aaataacagt aaatctgtat acttcatcaa
     3541 ttcttgaaaa cgtgaaaaga aaggttcttc atacgtaaaa ggttttccgc atgtatccaa
     3601 tgtagtatgc atcttccgct cttttgcttt tttaaatagt tcgatcaaaa aatcgatctg
     3661 caatagaggt tctccaccac taacagtaat accaccttta tctccccagt aaggtctgaa
     3721 gcgttctgct ttatctaaaa gttcatctgc tgtgtattct ttccctccgc caatattcca
     3781 agtatctgga ttgtgacaaa attggcaacg catgcggcat ccttgcataa acacgacaaa
     3841 gcgcagtcca ggtccgtcta ctgaaccgaa agtttcgata gaatgaacat agccgattgt
     3901 tttttcttcc atttaagttc acatcttttc tttgatttca ttttaagtac aagttagctg
     3961 tcgaagcgaa gctttctgaa caaaattttt cgcaattgcc gcttcgttat gtgtaaaatt
     4021 tacagggacg gaacatacaa gtcctgcccc tgtaaatcgt ttctttcaaa taagcatact
     4081 gattattcga ttttgatgcg atggctattc aacgtccttt cgacgctaag tcgaaaaact
     4141 tttttcacga actttttaca ttctgtcgtg tgaagttctc atcaagacat ctaattgttg
     4201 ttctcttgtc aagtcacgga atttaactgc ataaccagat acccggatcg tcaagttcgg
     4261 atatttcatt ggattttcca ttgcatcacg caataaatca ttactgaata cattgacatt
     4321 caaatgataa gctccttttg agaagtatcc atctaagaca ttacgcaagt tttcgattct
     4381 tgtatcttgt tcttttccaa gacctgaagg attgattgtt tgggtatttg aaattccatc
     4441 caatgcacag ccgtaattca ttttagctgt tgagttcagg ctagctaata acccactttg
     4501 ttccgcattg taacttggat ttgctcctgg cgcaagtggt tttccagctt cacggccatc
     4561 aggagtattt cctgtatttt taccgtaaac aacatttgaa gtaatcgtca ataatgaagt
     4621 tgttggtttt gagttgcggt aagttttatg acgtttgatt tgtcccatga agtattccaa
     4681 gatccatgaa actaattcat ctgcacggtc atcatcattt ccgtatttag ggaagtcgcc
     4741 atccacttca aaatcaactg ctattccttt ttcattacgg attgctttta cttttgcata
     4801 tttgatcgct gacaagctgt ctgccgcatg agagatccca gcaatacctg tagcaaatgt
     4861 gcgttgcaag tcagaatcca tcaaagccag ctgagctgct tcataagaat atttatcatg
     4921 catataatga atgatgttca acgtattgac atatagttct gctaaccaat ccatcatatt
     4981 catgaagtta tgtttcactt cttcaaagtc tagtatttct gatgtgattg gacgatattc
     5041 tggtccaact tgggcgccag tcacttcatc gactccgcca ttgatggcat ataatagtgt
     5101 tttagccaag ttagcacgag cgccaaagaa ttgcatgtct tttccgacaa ctgttcctga
     5161 tacacagcaa gcaatcgcac aatcatcaga accccattgt tcacgaagct gattatcatt
     5221 ttcgaattga atcgaagaac tttcgatagc gattctgcta gcatatgttc tgaatccttc
     5281 tggaaggtgt tcagaataca aaacagtcaa gtttggttct ggtgaaggtc ccatatttgt
     5341 taatgtatga agcagacgga aatcattctt tgtaactaat gaacgaccat ctaatcccat
     5401 acctgcaatg gataaagtag cccagatagg gaatcctgag aataattggt tatattcagg
     5461 tgtacgcgcg aatttgacca tacgtaattt cattactaag tggtcgatca attcttgtgc
     5521 ttctttttct gtgattactc cattatccaa atcacgttgg atatagatat caaggaatgc
     5581 tgagatacgt ccgattgaca tcgcagcacc attttgtgat ttgattgctg ctaaatatcc
     5641 gaagtatagc cattggacag cttcttttgc atttgcagct ggtttagaaa tatcatagcc
     5701 atatttcgca gccatttctt tcatttgcgc taatgcacga tattgttcag tgatttcttc
     5761 acgtaaacga atcacttctt cactcattgt atgataacca gtcatatcat gatctttttt
     5821 cttttgttcc atcaaatagt cgattccata caatgccaca cgacgatagt caccgatgat
     5881 acgtccgcga ccgtaagcgt ctggaagccc tgtaatgatt ttgtttttac gagcagcacg
     5941 catttcagca gtataagcgt caaatacacc ttgattatgt gttttgcgcc agtcagtgaa
     6001 aatatgtgtc aattcatcgt ttggtgtata gccattgctt tttagcgcat tgttagccat
     6061 attgattccg ccgaacggca tgaatgcttg tttcaaaggt ttgtctgtct gtagaccgac
     6121 gattttttcc aaatctttat ccaaatagcc tgcatcgtgt gaagtaatag ttgatacgat
     6181 ttctgtgtcc atatcataga caccgttacg ctcgaattgg atatcattta acttttgcaa
     6241 ttcatcccat aatttttctg ttgcttctgt tgggccttcc aaaaagctgt catcaccgcg
     6301 atattcagtg tagttatttt gaataaagtc tctagtgtct acgccttctt tccatttggt
     6361 acctttaaat ccattccatt gctccattgt atggacctcc taaatttctg ttttatgtaa
     6421 cagtttttac gtgattagta taacacagca ccctatcttt gcaagggttt tcttgttttt
     6481 tatttaacaa tttttatgtt ttatttacat atatttaaat caacgttgac ataccaacat
     6541 ttaagcattg tgaacacttt catctttttt tgcaaacgta taaggattta ttaaaaatga
     6601 atgaaaaaga gacgccacct gtattaatac agatggcgtc tcttcattaa aatactacaa
     6661 cagttacgac tcgatgaaaa tctcaggagc atcgataact tcgatgactt ctccaccttt
     6721 tttctcatcc atcgcaaatg atccattcga tgttcgatca cctataggga aatcagttgg
     6781 gtcaatcgtt aactgctgcc ctttttgatt catcaaggtt atttcttttg atcttccttc
     6841 cgacatatag ataatacggt gcggattttt tttcagctca cgcatgaccg tcactccccg
     6901 cttcgcacgt gaagtttggc tgatttcttg agcaagcatg cgtttaacag ctcctcgctg
     6961 agtaacgatt acgatcggcg tatcaccttc tgctaacaca agcagcccgt tgataatcct
     7021 atctccatct ttcaaattca tagacttcac tccagctgcc tttgctccaa caacgggaac
     7081 atctgttaaa ggatagcgca aacccattcc ttgatgcgtt actaagaaaa catctaagcc
     7141 tggctgatct tctttcagat acacagcaat cagttcatct tcttccgcct ttagcttcat
     7201 acagctgagc ggacggctct tataggtgcg ccatggttcg aactctgtca ttcgtgtttg
     7261 cttgatcaat ccattcttgc tgatgaatac aaatgttttc tctgcttgaa ttttttgata
     7321 tgggaaaaca gctaaaatcg attcgtctac agacagattg gagattgttt gagaaatgtg
     7381 ctcaccagca tctttccatt tcaaatcggg tagttcatgg acagggcggt agatcacatt
     7441 tgctttattt gtgatcagaa gaacatggtc caatgtactc aattcacctg agtatagcaa
     7501 gtagtcgcct tctcgcatcc cgatttcttc tggtttggaa gcactataag aacgaaggct
     7561 tgttcgcttc acatagcctt ctcgcgtcac actgacaatt acgtcttctt gtgccactaa
     7621 gacttgcgta tcaattttga tttcttcgat ttcgtcttcg attgtcgtta gacgagcgga
     7681 agaataattt tttttcactt ctcgcaattc ttttttcata acagagaaaa gctctttgtc
     7741 atttgaaagg attttattca actctgtgat ttgcgcaatc aagctttcgg attccttttg
     7801 aagctctgtg atatccgtgt tcgtcaaacg gtataattgc aatgtgacaa ttgcttctgc
     7861 ttgttcttct gtaaattgaa agacgtcgac taaattcttt ttggcgtctt ttttgtcttt
     7921 actttcacga atcgttgcga ttacttcatc cagaatcgat aaagctttca tcaatccttc
     7981 tacgatatgc tgtctttttt gagctttggc taaatcgaat tgactacgtt ttgttatgac
     8041 ttgctttcga tgctcgatat aacttcttaa aatgtctttc agtccaactt gatgcggagt
     8101 catatggtcg atagcgacca tattgaaatt gtaattgatc tgtagctcag tgtttttgaa
     8161 caaataattc aaaattcctt gagcattggc gtcttttttc aattcgacga caatttgcag
     8221 acctgttcta tcactttcat ctcgtacttc agctattcca tcgattttct tattcaaacg
     8281 gatctcatcc atttttttca ccaaggtcgc tttatttact tcatatggga tttctgtgat
     8341 cacgatttgc tgtttattac ccttcattgc ctctatcttt gttttcgaac gaagaataac
     8401 ttttcctcgt cctgtttcat aagctttttt gatttcatct tttccttgca agatccctcc
     8461 tgttggaaag tcaggtccag gaatatattc catcaatttt tcaaggctcg cttgtggatg
     8521 atcgatcata tagattgtgc catcgatcac ttcagctaag ttatgagtcg ggatttctgt
     8581 tgcatatcca gcagatatac ctgtcgatcc attgacgaga aggtttgggt atcgagctgg
     8641 aaggactgtt ggttcttttt ctgtatcatc aaaattccac acgagatcga ctgtttcttt
     8701 ctcgatatct gctagcatct ctccacttaa cttggataat ctggcttcag tataacgcat
     8761 cgcagcaggt ggatcaccgt ccatactccc gttattcccg tgcatttcga tcagtacttc
     8821 ccgcaacttc cagtcttggc tcatgcggac catcgcttca taaatactga tgtcaccatg
     8881 aggatgataa ttccccatga tatttccgac agattttgct gatttacgga atcctttgtc
     8941 atacgtattg ccatctttgt tcatggcaaa taatatccgg cgttgcactg gcttcaatcc
     9001 atcacggata tcaggcagtg cacgttcttg gataatatat ttggaataac gtccgaagcg
     9061 gtcacccatc acttcttcta aagttaattc ttgtacttct tgtcgatttt ccatcaggtg
     9121 acctcctatt ctacttcaaa taactgattg ttttcatttt tatctgctcg ttcttcatct
     9181 aataaatcat tagaaactga aggagagatt tcagtatcct cttttttatc taagatactg
     9241 ccatcttctt cgagcgtaaa ttgcacgtga ttttcgatcc attttcttcg aggttctact
     9301 ttgtcaccca tcagtgtggt tactcgacgt tcagcttgcg cagcatcatc gatacggacg
     9361 cggatcaacg tacgggaagt cggatccatc gttgtttccc acaattgttc cgcattcatc
     9421 tctccaagtc ctttatatcg ctgcagcata tatcctttgc cgactttttt aatcattgct
     9481 gccaattcgt catctgtcca cgcatattca atgacttgct tctttccttg tcctttggat
     9541 actttatata aaggcggtaa cgcgatatat actttcccag cttcaatcag cggtttcata
     9601 taacgataaa agaaagtaag cagtaatact tgaatatggg cgccgtctgt atccgcatcg
     9661 gtcatgatga ttactttgtc gtaattacag tcttcgatag aaaactcagg accgactcct
     9721 gcaccaatcg tatagatcat cgtattgatt tcttcatttt tcaagatatc ttgcatttta
     9781 gctttttcgg tattgatgac ttttcctcgt aaaggtagaa ttgcttggaa ctttctgtct
     9841 cttccttgtt tagcagatcc gccagcagaa tccccttcga ctaaatacaa ttcatttttc
     9901 ttagggtttc gggattgtgc gggtgtcaat tttcctgaaa gaagagattc gcctttttta
     9961 cgcttcttcc catttcggct ttcttctctg gctttacgtg ctgcttctct cgcttctctg
    10021 gctttgattg ctttacggac caacatctgg ctcatttcgc tattttcctg caaataaaag
    10081 cccatttgtt cactgatgac gttatctact acggtacgag caactggcgt accaagtttt
    10141 tcttttgttt gcccttcaaa ttgcagaaga ttttcaggta cacggataga aagtacagca
    10201 gctaaccctt ctctgaaatc actaccttcc agattcttgt cccgttcttt cagcaaacca
    10261 acttttctcg catattcatt ataagatttc gtcatcgccg ctttcatacc agcttcatgg
    10321 gttcccccat cttttgtccg aacgttattg acaaaagata aaacattttc tgaataaccg
    10381 tcattgtatt gataagcgac ctctacttcg ataccttctt tttctcctga aaaataaaca
    10441 actggtgtca atgtatcttt ttcttcattc agataatcaa caaattcttt gattccttct
    10501 tcgtaatgaa aaatttcctt gactggttct tctccacgaa gatcagaaag agagatttta
    10561 acgcctttta gaagaaaggc agattctctt aaccgttcgg ctaaaatttc atatgaaaat
    10621 ttcgttgtac tgaatattgt atcatctggt aaaaaatgga cagtcgtccc gtttgctttt
    10681 tttgtttttc ctatcttttt caaagtaccg tcaggtttac cgccgttttt aaatttctgc
    10741 tgatactcta cgccatctct aacgatcgtt acaattagcc atttcgataa ggcatttacc
    10801 acactggctc ctacaccgtg cagtccacca gaagttttgt accctccttg accaaacttt
    10861 ccgccagcat gcagcacagt aaaaatcact tcaactgttg gtattccgga agcatgcatt
    10921 cctacaggca tcccacgtcc gctatctgca acagttatac tattgtcttc atggatggtc
    10981 acatcaatct cactgccata acctgataat gcttcgtcaa ctgcattatc gacgatttca
    11041 tagactaaat gatgcaagcc tcggctgtca gtggatccaa tgtacatccc tggtcttttt
    11101 ctaactgcct ctaatccttc caatacttgg atggacgagt cattgtattc gttgtttact
    11161 ttttttgcca aggcaaacgc tccttcaata atactttgcg gcaaaaccgc accacgatta
    11221 tcatactaga aaaggtcgtg aaaaaaaaga gggtagacga gaaatcaatt ttaattatct
    11281 cctaaccgct ttttcgacat tcccctctat cgatcatctc ttctttgtgg tcatttttat
    11341 ttttggtttt ctttcaactt accaagttca atcttaatac aacgatccat tacgatatct
    11401 gtacgtcctg cttttcttag catctcagct gcttctttgt tttcaatccc taattgtgcc
    11461 cagaacactt tagcatcagt tttcaaaaaa tcacctgcta tttcaggtaa aaattcactc
    11521 cgtctaaata catctacgat atcgattgga tcatcgattt ctgtcaaatt cgcatagact
    11581 ttctcgccta gaatttcttc accagccaat aatggattta ctggtatgat tttatatcca
    11641 ttgtcttgca atacctgtgc aaccatgtaa cttgttcgtt ctggtttatt gctcaagcct
    11701 acgacggcaa tattttttgc ttcttccaaa tagttgaaaa tcgtttcttg tgttggattt
    11761 tttacagaca tttctgccac cccttgcttt tctttttatt ccatctatta tacttgtatc
    11821 tttcattttt tcaattgttc ctgttaccta ttcatgctaa aataaaggat agttttcaac
    11881 ttgaaagagg gtaaaaaatg aaattgatct tattacttat tttagcatat cttttaggtt
    11941 ctattccttc cggtgtctgg attggcaaat tgtttttcaa aaaagatatc cgacaacacg
    12001 ggagcggtaa tactggaacg accaatacat ttcgtgtatt aggcaaaaca gccgggattg
    12061 ttgtcctgct tatggatatt ttaaaaggta cattggctac ttgtttgccg atgatcttcc
    12121 acctttctgg gattaaccct ctttggtttg gggtatgcgc cattcttggc catacatttc
    12181 cgatttttgc tgggttcaaa ggaggaaaag ctgttgcgac aagtgcagga atgcttttag
    12241 gatttaatcc tgtatttttt gtttattctt gtatcatctt tatcgttagt ttattttgta
    12301 caagtatggt gagtttgagc agtatgatca gtgcagtatt gatcacttta tctacgatcg
    12361 tccttcctta tgtagctcct gtgattttgg ctaagcccaa ttggttactg acgatcattg
    12421 cctttttagt ttcttccttt atcttttatc ggcataaaga caatatcaag cgtatcagaa
    12481 accatactga aagtcgaatc ccgtttggtt tgaatagcag caagaaaaaa tcctgatact
    12541 ttttcattcc aaagagataa aacgggagtg tgacaaatcg atgtcacact cccttattac
    12601 ttacaggata gcttttttac acaccttggt aaacggtgtt ccctgctaac ttctcggtca
    12661 caacctctta cttcactgtg atggaatatt ttgtcttgaa gatttcatgg gcacctagtt
    12721 gattgatccc tactttttcc atcaagtttc cactactgtt caccgtgtca gcgattccgc
    12781 accacggttc gatacaaacg aaaggcgctt cttttggata aggagaccag atccctacat
    12841 aaggcatatc tttatagctt aaagttacgc tatgggaaga tttttcactt cgaatggcgt
    12901 acgcatttag accccttgtt tcaaaaacca aggcatcttg ctcaaaaagt tcacgattca
    12961 aagccagatt cgtatttgtc tgacctaacg tcttttgatc catatcgatg aatgggcctt
    13021 tcaatggtag gcgtaatctt gatttcaatg gagaaaatgc cagatagaaa tcttcaaatg
    13081 acaatccttc ttcaagaggt acattgaaag caggatgtcc accgatagaa aagaacattt
    13141 ctttttcacc ggtatttgcc acttgataat gaaccgtgat gccgtctcct cctaattcat
    13201 agctgatcac taattcaaaa tcaaatggat acttttctaa tgtctccggc gtactcctta
    13261 agatgaaact agccatttct tgtccatgtt caatgacttc aaaatccata tcccgagcaa
    13321 atccgtgttg ccccatttga tatgttttcc cttgataaac atactgatca tcttttaaac
    13381 gtccaacaaa agggaataaa accggtgcat gtctgcccca atacgctgga tcaccttgcc
    13441 agatatactc taaaccatgt tcttttgatt tcagactgat caattccgca ccgtgttcag
    13501 cgatttcggc gatcagctgg tcattttgaa taataacagt cattgccatt cctccaactt
    13561 cgacttactt tttaaaatgg gaaggctgga acaaacattt ctatctgccc cagtcccctt
    13621 acttctaata actgtgtttt gctaactcgt ctttaaaacg ggaattcaat acttttaaat
    13681 aagttccctt catgcctaac gaacgagatt caatgattcc agctgattcc aattttcgta
    13741 aagcattgac gatcacagaa cgagtgatgc cgatactatc ggcaatatta gaagcagtca
    13801 gacgaccttc atctccatcc aatgcttcaa agatagcttg tacagccttc aattcgctat
    13861 aagataatgt gttgattgcc atttggacag ccgttgcact ccgtacatct gcttcgatgc
    13921 ttcgagactt ctgataaagg atctgcatac cgacaaccgt agctccatat tctgctaaga
    13981 ctagatcgtc atctgcaaac gattgttcca tacgagataa gataatcgta cccaaacgtt
    14041 cgccagctcc aaagatcgga acgacagtcg tcagaccaaa tggatatttc tctcttgact
    14101 ctaccggaaa tgcagtaata tcactagtaa ttgggatatt agcttcagtt ttcatcaact
    14161 gtgccactgt atcagtataa gattcaggaa attgtttctc aacaaacatt gttttgattc
    14221 gttcattgtt cacatcgtga cgttcattat aacctagtaa tactccgtca cttcctatga
    14281 tataggtgtt gctgtcaaga ataccaccta gtgtgacagc cattttatcg taaggcaaat
    14341 ctgcttgcat gtcaaaagta ttcttttgct gcaaaagtgt gtttatcttg cgggttttcg
    14401 ttagcaagtc tgtcattgtc gtcctccgat tttttataaa atatagcgac ttaaatcttc
    14461 atttgccgca attgagccaa gtttgtcatc cacatatgct tctgtgatcg ttatttctcc
    14521 catttgcata tctggtgctt caaataatag atcttctagt agtttttcca aaatggtatg
    14581 cagtctgcgt gcgccgatat tatctgtgtc acggttgaca tcgaaagcga tttgagcgat
    14641 ccgttcaatt gcttcttttg taaacgtaac tgacacattt tctgtaccga tcaacgcgac
    14701 gtattgtttt actaaagcat tatttggttc ggtcagaatc ttcacaaaat cttcagctgt
    14761 caaatcatct aattctaccc gtatcgggaa gcgtccttga agttctggaa ttagatcact
    14821 tggtttagac aaatggaaag ctcctgaagc aatgaatagg acgtgatctg tttggatgat
    14881 cccgtatttt gtgtttactt gtgaaccttc gacaattggc aatatgtctc tttggacgcc
    14941 ttctctggat acttctccag attgctgtga tttagaagtg atcttatcga tttcatcgat
    15001 aaaaataatt ccactttctt ccgctaattt gattgcctca ctatggatat cagcatcctt
    15061 gactaatttt gctgattctt ccttgatcaa taactctaaa gcttctttga ctgttaccgt
    15121 ccgttccact tttttcttcg gtgtcaaagc acctagagtt tcatttaggt cgatccccat
    15181 ttgctccaat ccattgttca tcattggtgc aggtttcttt tggtcttcaa cttcaatggt
    15241 tacttcacga ctatccagta aacccttttg tagttgttcc aagactgtac ggcgattgat
    15301 tcgaatatct tctgtcactt cttcttgcgg ttcagcggta ttttgctgac tagcattaaa
    15361 aagattcatc atttgttcaa attgattagt ggattgtttc tgttcttttt tgataccagg
    15421 taccaagatt tttaccaaac gtttttctgc atttttttta gcacttgtgt atacacggct
    15481 gtactgctct ttttctacga tttggatcgc attttctact aaatcccgaa ccattgattc
    15541 tacatctctt ccgacataac cgacttcagt aaattttgtt gcttcgactt tgacaaatgg
    15601 tgcattgacg atttttgcca aacgtcgtgc gatttctgtt ttaccgaccc ctgtaggtcc
    15661 gatcatcagc atattttttg gtgtaatatc ttgttgcatt ttttcatcta actgcaaacg
    15721 gcgatagcgg ttacgcaaag caacggcaac agattttttt gctgcgtgct gtcctacgat
    15781 atattcatct aattcactta cgatttgtct tggtgtttgt tttaattcat acatatctac
    15841 tcacctctta cagttcttct acgataatat tatgattagt aaagatacag atatctgcgg
    15901 caatatttaa cgcattttct gcaatttctt tcgcactcat gtcgttttga gcatagtttt
    15961 tcattgctcg ggctgcagcc aacgcaaaat tccctcctga tccgatagct aagataccat
    16021 catctggtgt gataacttct cctgtacctg aaacaagaag catttcttta tcattcatta
    16081 cgatcagcat cgcttcaagt ttttgcatag attgctgtgt ccgccattct tgagctaatt
    16141 cgacagctgc gcgttgtaga ttcccgttat attcgtttag tttgccttca aatttttctt
    16201 ctaaagtaaa cgcatctgca acacttccgg cgaaacctac aacaacttct ccattgtaga
    16261 tacgtcgtac tttctttgca gtgcctttca tgacaacaga ttcacccatt gttacttggc
    16321 catctccagc catagcaaat ttcccgtctt tttcgacggc acagatagtg gttgaatgaa
    16381 atggtgattc gaccatgttt gtttcctcct aaatttgttc ttttccttat ttttacgctc
    16441 ttggatggaa agaacgatag tttttttgta aactttcttt tgttacatgg gcatagatct
    16501 gggtagtcga taaattggca tgtcctagca attcttggac tgtccgcaaa tctgccccat
    16561 tatttaataa atgagtagca aatgtatggc gcagcatgtg aggatggata tcactatcca
    16621 aactgctttt tttgatcatt tgattcaaga catattcaat tccagtaggc gtgattggtt
    16681 ttccatgatg attcacaaaa acgatatcat gctcttcgtg atattttgtc atcaatacct
    16741 ggcggccttc cctgaaatat tcttgcagag catcttgagc aaaagaacct agaggagcat
    16801 atctctcttt gttcccttta ccatggatca acatgacctc tgaatcaaaa tcaatgtctg
    16861 ataccgacaa actgctacac tcacttaaac ggatgcctga cccgtaaagg acttccagta
    16921 aagcgcggtt cctcaaatcc aaaggactat ctcctgctac gctgtcaaaa agtgcttgca
    16981 tttcattttc ataaaaaaaa cgtggcagtc tcaaattttt ctttttgaga tgaacgtaag
    17041 aaaatggatt ttcttttatc acttcttctt tgagaagata ctgataaaac gaacgtaggc
    17101 tggctatctt gcgactgata gaatttctgc tatagctagg acgatgatca gctaatgcac
    17161 tcaaatagac acgaaccgta aaatattccg ccttcagcag atcagcgtct ccagattcct
    17221 ccaaaaaatg tttgaaatgg agaatgtctt cttcatatgc ttctttcgtt ttttctgaat
    17281 atcctctttc gacaatcaaa taacgcaaaa atttttgttc ccacgtggct tctgtcattt
    17341 tctcaccttc cttttgtccc agagaataat ttagcacagc gtttataaaa agacaaataa
    17401 attgtcagaa tttaaccaat atttctaagt ttgttcacaa ttggcgcaat tcctcacatt
    17461 ttgtttgaga aattcttaag aaaaacgcaa gaaaaagagc gagactatcc gtctcaccct
    17521 cttcatggag tttccttact catttgggat tgcagttttt tctaattctg tttttattgc
    17581 ctcattagct tttaaagctc gttgtgcaat tgcttcatat cttgcttttt tgtctcgaat
    17641 gcgctctggc agttcaggaa acagaccata gttggcattc atcggttgga aatgttttcc
    17701 ttccgcatga gtaatgtagt aagccatact tccaatagct gtttcttgtg gaaactcgac
    17761 tggttccatt tcttttgcta agcgtgcggc gttgattcca gcaagtagtc cgctcgctgc
    17821 actttcaaca tagccttcta caccagtcat ctgacctgca aagaacagat cttcgcgttt
    17881 cgttgattgg taagtcggtt tcagcaattc tggcgaattc ataaagctgt ttcggtgcat
    17941 cacaccatag cgaacgaatt cagcattttc taacccagga atcatccgga aaacacgttt
    18001 ttgctcgccc catttcaagt gagtctgaaa gcccacgatg ttgtataaag aagctgcagc
    18061 attgtcttga cgcagctgga taacagcata aggacgtttg ccagttttag gatcttccaa
    18121 tccaactggt ttcataggtc cgaaaagcat cgttttgaat cctcgctgtg ccattacttc
    18181 aattggcata catccttcaa aatatttttc tttctcaaaa gatttaagtt ctgccacttc
    18241 ggcattgatt agtgcctcat ggaaagcttt gaattcctct tctgtcatcg gacagttcaa
    18301 gtaagctgct tctcctttgt catagcggga cttcaaatat actttatcca tatcgatcgt
    18361 agctttgtct acgattggtg cagctgcatc atagaaataa aaaccatctg aaccattgaa
    18421 tgctttgatg ctttctgcta atggatgaga agtcaaaggt cctgttgcta ctacggtgat
    18481 tccttccggt atttcagtaa tttcttcgtt tatcactgtt actaatggat gctctttgac
    18541 ttttcgggta attgtttcag aaaaagaatc gcggtcaaca gctaaggcac caccagctgg
    18601 cactgctgtt tcatctgccg aagtgattac taccgaattc aatcggcgca tttcttcttt
    18661 caataaccct actgcattag ccaaactgtt tcctcttaat gaattggaac atactagttc
    18721 agcaaaatta cttgtgtgat gcgcctcagt cgatttgact ggacgcattt catataaacg
    18781 gacagggact cctgcttgtg cgatctgcca agcagcttca cttccggcaa gccctgctcc
    18841 aacaacattt accattttca atgaaagaac ctcttttcta ttatttttgg atgtcttctt
    18901 cgtaatctcc atttacacag actacttgtt taccgccttt gactttcttc tcgaccagat
    18961 atccgtcaca ttttggacaa tttctgccca ctggtttatc ccaagaagtg aattcacatt
    19021 ctgggaaacg ggaacaacca tagaatagtc tgtttttctt agattttcgt tcaatgactt
    19081 gtccttcatg acatactggg caagtcacac cgatttcttt tacgattggt ttcgtattcc
    19141 gacaatcagg aaaattactg caagcataaa acttcccata tttccctaat tttatcacca
    19201 tcggatgtcc acagacatca caatcgaatc ctgctggttc atctttgatt tgaatttttt
    19261 cgattttttc ttccgcattc gtcagttctt tttcaaacgg tttatagaaa cgatcaacca
    19321 ctttgaccca cgcttccgtg ccttcttcaa ttttatccaa atctccttcc atttctgctg
    19381 tgaaatggat atcgacgatt tgagggaaga attcacagat taatgaattg acaatttctc
    19441 ctagttcagt cggctcaaat cttttttgtg tcaattttac atagtagcgt ctttggatcg
    19501 tatcaagtgt tggtgcataa gtggaaggtc gccctacccc gttttcttct agtgcacgaa
    19561 tcaacgtagc ctcactaaaa cgtgctggtg gttgagtaaa atgttgtttt ggttcaatat
    19621 caacagattg gactacatcg ccttcttcaa gttctggaag aatattttct ttctcttctt
    19681 tgccgtcatc tctaccttcc acatacactt gcatgaatcc tttgaatttg atttttgaac
    19741 cgttcgcaat gaagatgaca ccattttgtt ctaatgtcac tttcattgta tctaaaatag
    19801 ctggtgtcat ctgactagcc acaaaacgtg accagatcaa tgtataaagt ttcaattgat
    19861 ctttatctaa atattgtttg agttcatctg gtgtacgaag cacactcgtt ggacgcacag
    19921 cttcatgggc atcttgtgcg ccttgagcat ttttgatctt tcggtgatca tgtctagaaa
    19981 attcttttcc atacgtatct tcaataaatg ctgcggcctc tgcttttgct gtatcggcaa
    20041 tccgggtaga atcagtacgc atgtatgtaa tcaagccgac tgttccttgt ttacccaaag
    20101 tgattccttc atataattgc tgtgctacca tcatggtttt tcttgttcga aagtttaact
    20161 tacgggcagc ttcttgttgc aggctacttg tagtgaaagg attcgcaggg tttcttttgc
    20221 gctctttctt ttctactttt ttaacttcga agtctgttcc atcaattcta gcagtgactt
    20281 cttttacact ttgtgcatct ggtaatttct ttttcttacc gtcgattccc cagaaatttg
    20341 ctttgaattt cttgcgagct tttttaaaat taccgtcgat gctccaatat tcttctggaa
    20401 caaattctcg gatctctttt tcacggtcaa taataatttt taaagcgaca gattggacac
    20461 gtcctgcact taaccctttc ttgaccttac gccagagaat aggactgatc gaataaccaa
    20521 cgattctgtc taaggtacga cgagcttgct gtgcatctac taaatctaca tcgatcgttc
    20581 ttggttcctt aaaagctgct ttgactgcgt cttttgtgat ttcattgaag accacccgat
    20641 ttttttcttt cagatccaac ccaagtaggt acgctaaatg ccaagcaatc gcttctcctt
    20701 ctctatccgg gtcacttgcc aagtaaactt tttgagcttt ttttgctgcg gctttcaggc
    20761 ttttgatgac atcgcctttt ccgcgtatag aaatataatg gggttcataa ttgttttcaa
    20821 aatcgatacc cattttactt ttcggtaaat ctcttatatg gccaacactg gccattactt
    20881 tatagtttcg tccaagatat ttctcaattg tcttggcttt tgcaggtgat tctacaatca
    20941 ctaaatattt atacgccatt actgaatgga ctcctttcca ttgtcgtgtt cctactatta
    21001 aaagaggatt catacaaatc gtagctaatc atatccattc agaaaacgtt tgtcaagaaa
    21061 agcaaatgaa aagtatggtg ttattcttta aattctgcca agatatcttc acccgaaacc
    21121 gtgcaaactg caccgtcttg gatcaaatga tgacatccgg tagaatgttc tgttaaaata
    21181 tttcctggaa cagaaaaaac tgttcttcca agatccaatg cttgctgtgc tgttatcaga
    21241 gagccgcttt ttttcttggc ttcaaagaca catgttcctt tagacaaagc tgcgataatc
    21301 cgatttctca ttggaaaaca atgctttttc gggccggatt gaggaggata ttcactcaaa
    21361 agaagatgat catttgcaat cacctcttgc aagtcacgat tttcagcagg atagacttgc
    21421 tctatccctg ttccaatgac tgcgattgtc cgtccaccat gagagattgc tgcttggtga
    21481 ccgtatgtat ctatcccttt cgcgcagcca ctgacgatca catatccagc ttctatcact
    21541 ggttttagta aataataaac tgtttttttg gctaaagcag aagtgtgtct tccaccaaca
    21601 acgcttaaca tgttacaaga aagtaaagat agatcaccat tataaaacag tacaattggc
    21661 ggataggtca agtgaagcag ctgaggggga tatttgggat caaagcaagt gataaacgac
    21721 tgtttcgatt ggattttgcc ccagttagcg tgaatttcct gccaactttt gcgaaacagc
    21781 tcccggtgtt tggttatttt acctatctta atgatgtctt caaatccgat ttctttataa
    21841 ggatactgca tcatccagtt agcaagtgcc cattttcctt gactactgat tccttttgta
    21901 taagcaagtt tcaataaatt ttcttctatt tgatacatca taaaaccgcc tttcgtctct
    21961 ctttctaata agatacgtaa aagcggctgt tttttattgt aaatattttt ttacaggtgc
    22021 aaaactttta cgatggattg gcgtaattcc ttgtcgttca agtccttcta agtgttcctt
    22081 tgttccataa cctgcattat gcgagaaacc atatccagga taaagcacgt catattcttt
    22141 catcaattgg tctcgatata ctttagcaat gatacttgca gcagcaatcg acacagatcg
    22201 tgcatcccct ttgattattt tttcctgctg aataggcaaa tccaaggtca tcgcatcgat
    22261 caacaaatag cttggtacaa tggacaattt ttctactgct tggatcatcg ctaatttact
    22321 tgcttcataa atattcacat catcgattgt ctgctgatca acgatcccga tcccgattgc
    22381 cagggctttt tcttgaactt gttgataaag aagttctcgc tttttctctg ataattgttt
    22441 ggagtcattc agccctaata tgatctcatt tttcggcaag ataactgcag ctgccacgac
    22501 aggtccagct aatggtcctc ttcctacttc atcaatgccg gcaatccatt ggtgaccttg
    22561 tgcgtaccct tgcttttcaa agcaaaacat ttcttcatat ttttccttaa ttaatgcttc
    22621 ctttttcaat cgtcgttcaa aagaagccag tgcttgctgc acgcctttgc gtggatcatt
    22681 tcttaatgat tctagtgtag gatcatctat tctctgtatg cttttcaatc gttctttgat
    22741 actttggatg gattcagtca tcactttttt gctccatttc ttcgcaacga tccagtgtgt
    22801 atcgtccaag ttttccttga cgaatatcat gcacgactaa ttcgctgcca cgatcatagt
    22861 cttctttata cccacgtttt gctgtaatat ccatcagtaa ttccggtgcc aatttttctt
    22921 cttgctcgat tgataaacca taacgatcgc tgattcttcc tgaataatat cgggcaaaaa
    22981 agtctaatcc gtaaatcgct aaatcatcta aatggacaag ctggtctttg atcgcgcctg
    23041 tcagcgctag ttttttgccg atttcttcat cttcaaattt gggccataaa atccctggtg
    23101 tgtctaacaa ttccaactca ttacctaaac gaagccactg ctgacctttt gttacgccag
    23161 gcttatttcc tgtctgtgca attttcttgc ctactagtcg attcatcaac gtagatttcc
    23221 caacatttgg aatacctaag accattgccc gaatagcgcg aggcttgatc ccccgagcag
    23281 catctcttgc ccgtttttct ttcaatgctt ctttagccgc aggtactaga cctttaattc
    23341 ctttactttc ttgtgccgtg atagctaatg caagatggcc ttgtttacga aaatattctt
    23401 gccacagctt tgtctgttgt ggatctgcca gatccgcttt attcaataaa acaaggcgag
    23461 gtttttgctg aatgatctga tctagcattg gattacgaga agataaaggt aatcgtgcgt
    23521 ctaccaattc aaatacgatg tccacatatt tcattttttc agaaacttct cgtcgggctt
    23581 ttgccatatg tcccggaaac cattggatgg tcattgttct ctccattcct ttcataccgg
    23641 aatattcccc ggctttactt gtttttttct ttttcaatat aaattcactt ttttattata
    23701 aagaaattcc atctattagt taagtgaaag tgaaccagtc ctcattctat cacatcttca
    23761 cttttttgat gatgcaaatt tttattttgg tttctatata tgaatctaaa tttttgacag
    23821 cgtttcctaa ataagagatg atagacgaac aaaacagaaa ggagacgaaa caaatgaata
    23881 gtatggtttt tgttaatttt cctgttgctg atgtcaatcg ttccgttgct ttttatgaaa
    23941 agctgggttt taaaaaaaat gaagaatttt
//
LOCUS       DAYYME010000031        23594 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000031 DAYYME010000000
VERSION     DAYYME010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.031"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            27..983
                     /locus_tag="ACHBFW_001020"
     CDS             27..983
                     /locus_tag="ACHBFW_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM9317345.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1056..1172
                     /locus_tag="ACHBFW_001021"
     CDS             1056..1172
                     /locus_tag="ACHBFW_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFM9317346.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(1227..2909)
                     /gene="recN"
                     /locus_tag="ACHBFW_001022"
     CDS             complement(1227..2909)
                     /gene="recN"
                     /locus_tag="ACHBFW_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFM9317347.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(2927..3376)
                     /locus_tag="ACHBFW_001023"
     CDS             complement(2927..3376)
                     /locus_tag="ACHBFW_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM9317348.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(3483..4301)
                     /locus_tag="ACHBFW_001024"
     CDS             complement(3483..4301)
                     /locus_tag="ACHBFW_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFM9317349.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(4310..5200)
                     /locus_tag="ACHBFW_001025"
     CDS             complement(4310..5200)
                     /locus_tag="ACHBFW_001025"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM9317350.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(5202..5426)
                     /locus_tag="ACHBFW_001026"
     CDS             complement(5202..5426)
                     /locus_tag="ACHBFW_001026"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFM9317351.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(5431..6768)
                     /gene="xseA"
                     /locus_tag="ACHBFW_001027"
     CDS             complement(5431..6768)
                     /gene="xseA"
                     /locus_tag="ACHBFW_001027"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFM9317352.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(6769..7623)
                     /locus_tag="ACHBFW_001028"
     CDS             complement(6769..7623)
                     /locus_tag="ACHBFW_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFM9317353.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(7720..8169)
                     /gene="nusB"
                     /locus_tag="ACHBFW_001029"
     CDS             complement(7720..8169)
                     /gene="nusB"
                     /locus_tag="ACHBFW_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFM9317354.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(8162..8587)
                     /locus_tag="ACHBFW_001030"
     CDS             complement(8162..8587)
                     /locus_tag="ACHBFW_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9317355.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(8833..9897)
                     /locus_tag="ACHBFW_001031"
     CDS             complement(8833..9897)
                     /locus_tag="ACHBFW_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFM9317356.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(10069..10314)
                     /locus_tag="ACHBFW_001032"
     CDS             complement(10069..10314)
                     /locus_tag="ACHBFW_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9317357.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(10453..10827)
                     /locus_tag="ACHBFW_001033"
     CDS             complement(10453..10827)
                     /locus_tag="ACHBFW_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFM9317358.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(10910..11824)
                     /locus_tag="ACHBFW_001034"
     CDS             complement(10910..11824)
                     /locus_tag="ACHBFW_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9317359.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(11845..12648)
                     /locus_tag="ACHBFW_001035"
     CDS             complement(11845..12648)
                     /locus_tag="ACHBFW_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFM9317360.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(12681..13670)
                     /locus_tag="ACHBFW_001036"
     CDS             complement(12681..13670)
                     /locus_tag="ACHBFW_001036"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM9317361.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(13773..14264)
                     /locus_tag="ACHBFW_001037"
     CDS             complement(13773..14264)
                     /locus_tag="ACHBFW_001037"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM9317362.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(14455..17262)
                     /locus_tag="ACHBFW_001038"
     CDS             complement(14455..17262)
                     /locus_tag="ACHBFW_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9317363.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(17696..17989)
                     /gene="rpmA"
                     /locus_tag="ACHBFW_001039"
     CDS             complement(17696..17989)
                     /gene="rpmA"
                     /locus_tag="ACHBFW_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFM9317364.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(18002..18346)
                     /locus_tag="ACHBFW_001040"
     CDS             complement(18002..18346)
                     /locus_tag="ACHBFW_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFM9317365.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(18373..18681)
                     /gene="rplU"
                     /locus_tag="ACHBFW_001041"
     CDS             complement(18373..18681)
                     /gene="rplU"
                     /locus_tag="ACHBFW_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFM9317366.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(18701..18777)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            18963..19604
                     /locus_tag="ACHBFW_001042"
     CDS             18963..19604
                     /locus_tag="ACHBFW_001042"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM9317367.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(19666..21297)
                     /locus_tag="ACHBFW_001043"
     CDS             complement(19666..21297)
                     /locus_tag="ACHBFW_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFM9317368.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQFPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(21420..21779)
                     /locus_tag="ACHBFW_001044"
     CDS             complement(21420..21779)
                     /locus_tag="ACHBFW_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFM9317369.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            complement(21938..22011)
                     /locus_tag="ACHBFW_001045"
     tRNA            complement(21938..22011)
                     /locus_tag="ACHBFW_001045"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21975..21977),aa:Arg,seq:tct)
     gene            complement(22320..23003)
                     /locus_tag="ACHBFW_001046"
     CDS             complement(22320..23003)
                     /locus_tag="ACHBFW_001046"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFM9317370.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            complement(23131..23592)
                     /locus_tag="ACHBFW_001047"
     CDS             complement(23131..23592)
                     /locus_tag="ACHBFW_001047"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM9317371.1"
                     /translation="MLILLVVLVPRIFGLTPYIIEDKEMSPYYAQGNLIYVRPKNTNE
                     ILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIF
                     QIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
BASE COUNT         6845 a         5186 c         3925 g         7638 t
ORIGIN      
        1 aagaaacagg caacaggagg aaaatcatga tcaaatattt tacattagat aatgagaaac
       61 tgcgtaatac cggaaaggaa actgaccaaa cgatctggta ttgtgtagaa cgtcctaatc
      121 aagaagagat tcatcagctg acacatcaat ttgaaattcc gtcagattac atcacttcca
      181 tattggacga tgcagaaaat tcccgtacag aagaattcaa tcaatcaaag ttgacaaagc
      241 cagcgttatt attgttacaa tatccttttg ctaaaacaag tcctagcggt tattttcaag
      301 tggatacata cccgctctct attattttga cgcccaaaaa gaagcttatc acaattacga
      361 atcatgaacc actgttttta aagaaagtct ttagccagac ctttccaaaa agtgatattt
      421 cgcctaatct caatatcttc tttcaattca tttggcaggt cacactctct tacaacagtt
      481 atttagctac gcttcaatct caatgcgata ctttggaagc caaacttcaa gtatccactg
      541 aaaacaaaca actttatcaa atcatggata tccaaaaaag cctcgtttat ttcagagaag
      601 cgacttctgc taatttagat gcattgaaag aactagcgca aaataaactt ttccaagaaa
      661 atcatgcttt gaaaaatcat ttacgtgatg tattggtgga aacagagcag gcaatgacta
      721 cttctcgcat tcaattaaaa ctagtagaac aaatgaatca gactttttca gcaatcgtat
      781 cgaataatct gaataatatt atgaaaattt taacttcttt aacgattatt ttaactatac
      841 cgacaatcgt cggcagtatc tatggaatga acattaagtt acctattgct gaaagggatg
      901 atgcctttat atggctagtt ctattaacat tatttatttg tatcgtaact acctattatt
      961 taaaaaaagg gaagttttta taaaaaagcc ctcttgtgga aatggaaaaa agattgtaca
     1021 cttgaatcaa ctaattaatg acgaggttga aaacaatgcg tgaaaaaaaa caaacaagta
     1081 cttttgctaa agtaacaaaa tttgtcattt ggacaatgct gattttaacg attggatctg
     1141 ttgtattgac tgctgtaatg agtgtcatgt aaaaataaaa tatagctatc agctgctgaa
     1201 aaggacctga accactgcga ggtggctcag gtcctttttg cgagtttcaa tagttctttt
     1261 gcatgttcga gtgtgagttt tgtaatctca cttccagcca gcatacgtgc aatttcttct
     1321 tctctttctt ttgttttcaa ctctgaaaca gaagtttgcg tacgtccatc aatcactgct
     1381 ttcacaatat aatattgata gtcggcgact gcagcaactt gaggcagatg agtgatgcac
     1441 aaaacttgag agtatttcga tatttgagaa attttatctg cgatggcctg tgcaactctt
     1501 ccgctcacac ctgtatccac ttcatcaaaa ataatacttg tcacaccttg ctcagaagaa
     1561 aaaatagttt tcaaagcaag caacatccga gacagttcgc caccagaagc gactttcacc
     1621 aaaggcttca gtggctctcc tggattggtc gtaatgtaaa attcgatttg gtcaaatcca
     1681 tcatgattca attgtctatt ttcttctttt aagaaacgta cctcaaactc agtgttttcc
     1741 atatacagac ttttcaattc atgcagaatg gatttttcca attcttttgc gattttttta
     1801 cgcgcttcat gaagatctgc ggcttgttgc tgaagtagaa gctctttttg ctctagttcc
     1861 tctttcattt tgtccaattg accttctgaa aaatcagaag aagctagctc ttcggtgatt
     1921 tcgtcatagt aagccaatat tgactcaatg gattcgccgt atttacgttt caactgtcgg
     1981 attaattcta aacgttgcgt aacttcttct aaacgactct catctaactc caataaatca
     2041 atctgacgac tcatatcgcc gattgcatct tgcagcaaat agtaagcact ttggatattt
     2101 tcataaattg cttcatactc cacatctaga tgggcgatcc cttgcagttc tgaaacggcc
     2161 aagccaacac catctacact gttttgttca ttattcgtca gtgctccgta acctgcagct
     2221 aatccatcag caattttttg ataattgcta agtttttctc tttcttcttt taatttctct
     2281 tcttctccga cttgcaaatc agcagcagct atttcttctt gctggaaatg gagcatatct
     2341 attctctgaa cataggattt ttcattttgc tgcacttttc gtaccctttt ttccagctca
     2401 cgataagaga tatattcttg ctgataactc tttttcttct gttggaaagc tacatctcca
     2461 aaacgatcca ataaagcaag atgtgattca ggctggagca gttcctgatg ttcgttttgt
     2521 ccttggatat ctactaaata actgccgatt cttctcagat tagctaatgt gataatatga
     2581 ccattcactc gacaaacatt ttttccagtt aaggacatat cccgtcgaac aatcagattg
     2641 tcctcatctg tttcgatccc taattcaacc atcagttcag agaatccttc ttgttttggt
     2701 agttcaaaca acccttcaag cacacatttt tctgctcctt gacgtatata atcgcttgaa
     2761 cctcttcctc ctgctaacag acctaaagca tcgataataa tggatttacc cgcaccagtc
     2821 tctccggtca gcgcggtcat tccttcgtga aaagaaagcc gcaattccgg aataatcgca
     2881 aagttagtaa tactgatttc ttgtagcatc acattcgctc ctcaacttaa aggtaatgca
     2941 ggaagtcttt cttcaataaa actgcctggt cttcagttcg tgtaatcatc aaaacattat
     3001 cgtcatcatt gataatcgaa aataattctt tcttgtaacg tttatcaatc aaatttgctg
     3061 ctgccgaagc atttcccggc aatgttttca aaataacaaa tttttccatt tggtcaactg
     3121 ccacgaaagc gtcttttaat agtttttcca atttagtatt tacatcttcg tttgtttcag
     3181 ctggtatgct ataacggtag ccgccatctg aagccggtac cttgatcagt ttcagttcct
     3241 ttatatctct ggaaatcgta gcttgagtaa cagatattcc tttgcttttc aggatttcaa
     3301 caaattcttc ctgctttcgg atatcttgct cactcaacaa tcgagtaatc aaacgatgcc
     3361 gatctttttt tctcattacg tcttcgctcc ttagcatatt cttcttaatc aatgatagct
     3421 tatttccttg gtataataaa gaagaatccg catacttctc acttgatttt cacagttaca
     3481 atttatttat ggtcaaactg ttcgtgtgca tttgaaacca cttctgccat attaatagct
     3541 gaattgattg ttccgctttc gggtactttc ctcaagtgag ccaagaactc gatatttcct
     3601 tctccccccg tgatcggaga ataacttaat tccagcacat catatccacc ttgtgccgca
     3661 aatgacaaaa tgtcttctaa aactttttga tgggtctgag ggtcacgaac aattccgttt
     3721 ttccctacag cttctctgcc tgcttcgaat tggggtttga ttaaagcaat cacttctccc
     3781 tcttctttta aaatagcatg taatggaggc agcatcagtt ttagtgaaat aaaagaaaca
     3841 tcgataacag caacttctgg aattcctaag gtaaagtctt cgggttttgc ataacgaaaa
     3901 ttcactcttt ccattacgac gactctagga tcttgtctta ttttccaagc tagctgattg
     3961 tagccaacgt ctaaagcata actcattttt gcgccatttt gcaaagctgc atcagtaaat
     4021 ccgccagtgg atgcaccaat atctaacaat gtttttcctt caacagatag atcaaatgct
     4081 ttgagtgctt tttctaattt taatccccct cgagaaacat aaggaagttt tttacctttg
     4141 actcttaatt cactactcac tggaattttt tctcccggtt tatcaaaccg ctcgtttttt
     4201 tcgttgtaaa tcaaaccagc catcactgaa cgtttggctt gttctcttgt ttcaaataat
     4261 ccttgtttga ccgctaatac atctacgcgt tctttttcca ttctttccct catttctcca
     4321 catcaaattg cttgatcatc tggcgcatca gcccgccatc aaaagaaaaa acgtcctctt
     4381 ctaatttgtc tatgatcttg ttggcggata acagctcaat ctccaacgcc tcacgtgtct
     4441 tttctaggcc taataaacgc gggtaagtat ttttttccat acgttcatct ttaccagctg
     4501 ttttccctaa tgttttcgtt gtaccaatca catcgagtag atcatctctt atctgaaaag
     4561 ctaaacctaa atgttcggct aatcgttgaa gtagcaataa gatttcttca ggttgcttcg
     4621 ctaatatgcc accagccagc aaggcataac gaatcaaatg ccctgtcttt ctttcatgaa
     4681 taaatgctaa ttcttctaaa gaaagctttt tcgattcgcc ctcgatatct gcagcttgcc
     4741 cagcaaccat accttgtgat cccgcacaaa cagctaattg ctgaagcagc agaagcttag
     4801 gagaattacc taagtgtgcc atactgatta gctggaatgc tcctgtaagc aaaccatctc
     4861 cggccaatat agctaatgct tcgccgaata ctttatgatt ggtaggtttt cctctacgaa
     4921 gatcatcatt gtccatcgca ggcaaatcat cgtggatcaa ggaatacgta tgcaccattt
     4981 ctaaagcagc tgctacttga tagacgggaa catcaatagg ctcatcaaag gaagcaacgg
     5041 ccgttaaaag taataaggga cgtattcttt tcccgcctgc tttcactgaa taactcatcg
     5101 catcatagag tcctctatcc gttgtatgct catctaaaaa agacaagatt tctttttcta
     5161 cagcaggtag atgaatagct gaaaagtcag ttaatctcat ctcagctttc ctcgtttccg
     5221 tcaaaatcaa cttcttcatt tgattctgtc atcattttcg tcaaggtctc ttctgcttta
     5281 gctaatgtat cttggcaatg tttgcttaat tccattcctc gtttaaacga atctagagct
     5341 gattctaatg gaacgtctcc ttgttctaac tgcatgacaa ttttttctaa ttcttgcagt
     5401 gactcttcaa aagttggatt ttccatattt ttattctcct ttgtctatct cttgtacttg
     5461 tcctttaacg attccatctt gataatggat cgtcagcaaa tccccttgat gaatctcggt
     5521 gacacttttt actacttgat catttaacgt cgtatagctg tagcctcttc ccatgatttt
     5581 caatggactc aacaaatcaa gagattgaac agcttgagca aaacgctgct gctgttgttt
     5641 catatattgt tggattttct cttctaagcg tttttctaga taagcagttt cttggcgagc
     5701 tgctttaact cgataaagcg gagccacttg ttccaattgg tggcttaatg cgatcgtttg
     5761 tttttctttc gcatgataaa tttgctgtgt agcttgatat aatcgctgtt tcaactggtc
     5821 taatttgatg gtctgacctt catacaatct ttcaggctgc cgaaatacat aggaacgtcg
     5881 agcacgctcg aaccgttctt gtttgcgctg gatctggcgt acaaacgcct gctgcaatct
     5941 agcttgccgt tcttgaatgc gcatcaactc ttctgacaaa acaggaacag ctaattctgc
     6001 agctgctgtt ggagtggcag cccgaacatc tgctactaaa tcagcgatcg tggtatctgt
     6061 ttcatgtcct acagatgaaa tcaccggcgt attagcttga taaatcgccc gagcaacccg
     6121 ctcttcatta aacggccata aatcctcaat cgaaccaccc ccacgaccga tgatcattgt
     6181 atcgaaattg cccatctctt ctactctctg gatattcctg acaacattgt ccgctgcttg
     6241 gtttccttgt acaactgttg ggaacaaaac caattggaca attggatatc ttcttttcac
     6301 cgtcgtgatg atatcgcgga taaccgctcc gcttggactg gtaaggaccg cgattcgctt
     6361 tgggaaacgt ggcagcattt ttttaggccc ttgaaaaagg ccttccttca tcagcttttc
     6421 ttttcgttct tccaattctt gatataaagc acctattcca tcaggctcca tgtgctctac
     6481 atagatttga taagaacctc ccgcttcata taatgaaata cgcccaacga caaggacttt
     6541 cattccttct tttggctgga atcgtaattt ttggaatgct cctttgaaca taatagcaga
     6601 tatttttgca tggtcatctt ttaagctgaa atattgatgg gcattggcac gtaaacggaa
     6661 attcgatatt tcgcctgtta aatatacgcg ttctaaataa ggatcggcat caaattttct
     6721 tttcaaatac ttcgttaatg tagtgacagt taaatattct tgtgccatct attcacctgc
     6781 tccaaaagct tcggcagatt ttacggtttg atacatcagc atcgtgatag tcattggccc
     6841 tacacccttt ggcacaggag tgatccagcc tgcaataggc tccacttcat caaatttcac
     6901 atcaccgatc agcttaccgt tagcatcacg attcatccct acatctatca ctacagcacc
     6961 tggttttacg aattcttttg tgacaaaatg tccacgtcct attgctacga ctaaaatatc
     7021 tgcttcttgt gccagttcag ccagattttc ggttttagaa tgagcaacag taacagttgc
     7081 atctgccatc aagagtagct gagccatcgg tttaccgacg atattgcttc gtccgataac
     7141 tactgctcgt ttacccgccg gatcgataga ataagcttca aacattttca tgatgccgta
     7201 tggagtacaa ggaatcttgt ccggtttacc cgcaaggagt ctgcctaaat tcatcggatg
     7261 aaaaccgtct acgtcttttt cagggcggat agctaacagg atcttttctt catctatatg
     7321 ctttggcaat ggtaattgga ccaatatacc atgaaaacta ggatcttgat tgtatttttc
     7381 aatttctgca agtagttcgt cttcagaaat cgtctcagga taacgctcta ccaatgaatg
     7441 gattccgatt tcttttgcta cacgttcttt atttcttaca tagatttggc tggctggatt
     7501 ttcccctacc aataaaacga ctaatccggg atggatacca ttatctttca atttttggat
     7561 tttttcagca atctctgcct gcattttttc agctaattct tttccgttaa tcaattctgc
     7621 catctaacca ctcccagata ttttttgtgt tttcattcta ccatagaatt ttcccgattt
     7681 ataacaggtt tatgatgatt catagaaaaa agctaggact tacgccccag cttctaattc
     7741 tttcaataca ttggataaaa caccgttgac gaatttacgt gaacggtcat cactaaatgt
     7801 ttttgccaac tcgattgctt catttaaagc aacagccgct ggtacatctg tcacataagt
     7861 catttcaaaa atcgcgatac gcaagatcac caaatccatt tttgagatcc gattgatgtg
     7921 ccaattgttt tttaaatgtt tttcaatcac tttatccaat tcatcttttt ttgcacagac
     7981 accgccaact agcgtatcaa gataaacagg aacaaactct gattcatctt cgttgatcat
     8041 gtcgcggtgg tctaattcga ttgcgttgaa aatcgcatct tcttttgtta ggtctgcatt
     8101 gaagtctaat ggaaataaag cctgcaatgc catttcccga atctcgtgtc ttgataattc
     8161 tttattcatt ctcttcctct tcgtcttcaa aaagttcttc gattgttggt tgttctaatt
     8221 tttcaggaac aacagctact acatggatat tgacttcaga taaatcgata tccgtcatga
     8281 aaaggacttg ctggctgacc cgttcctgca tttccattgc tactttagga actgaaacac
     8341 catattctag ataacagtaa agatctactt tcaagccatc ttcctcgttt gctaagtaaa
     8401 caccttttcc gtgagctgca cgtcctaata attcagtgac attgctggca aaagttccgc
     8461 gcattccgta tacgccttct actttagaag ctgcaatccc gataattact tcgatgactt
     8521 caggagcgat cacgatctct cctaaatctt ggtttgcgtt taaaatcaaa tttttttcat
     8581 ctgccattta tttattcctc cctttgccta agcacgagat acatccgaat tctctatgat
     8641 gcggcatagt gatcacgtgg taagaaaact gccgccgatc agctgcaaac tgctatgaat
     8701 tggagaaaac acccaacttt attatctctt acagcttaaa agttcttcta tagtgtatca
     8761 ttaaatcgag tgattttcta taacaaagtc tgtgaacact gatttttacg cttacttctt
     8821 ctattgatga aactataaga tgatcaattc ttttggtgaa cgagtcaaca cacgatttcc
     8881 atcttctgtg atcaacaagt catcctcaat acgtactcca ccgattcctg gtaagtagat
     8941 ccctggttca tcagtgatca catttcctgg tacaaattgc ttttctgcac gagaagaaac
     9001 gtttggtcct tcgtggatct ctaaaccgat cccatgtcct gtagaatgtc caaatgcttc
     9061 cccgtaacca tgttttgaaa tataatctct agcaactgca tctaattgga caccagtcat
     9121 tcctggtttt gcagcatcaa taactgctaa ttgagcatct aagactattt gataaatttc
     9181 ttttaatttt tccccaggat caccaatagc gaatgtacgt gtcatatcgg aaacgtaccc
     9241 ttcgtaatag caaccaaagt ccaatgtgat caagtcacct tgttcgatga tttttttgct
     9301 tgctacacca tgcggcatag ccgaacgcaa tccgctagca acgatcgtat caaaagaaac
     9361 acctgaagcg ccaagagaac gcatataaaa gtctagttga tttgcgactt caatctcagt
     9421 catgcccggt tggatcattt tcaggatgtg atcatatgcc atatccgcaa tatgacatgc
     9481 tttttcgata atagcgattt cttcttcatc tttcacttct ctgagttctt cgatcatctg
     9541 tgaaacaggt actaattccg catcgatgat ttcttctaaa accgaatatt ctaaaaatga
     9601 gactgttgtt tcctcaaaag ctaatgagtg cagttcttct ttgcgtacta agtttgctac
     9661 ctcatcaaaa ataggtccga catttttaat aatttcaaac ccttgagcct gcgctgccgc
     9721 ctgctcggta taacgaaaat cagtaataaa aaatgctttt tccaaagtaa taacagctaa
     9781 tcctgtcgta cctgtaaagt tcgttaagta acgaagattg tacggactgg taaccaaaaa
     9841 tgaatccaaa ttttcttctt gcattttttt gcgcaatttt tctacacgta gcatcataat
     9901 gaaactccct ctttctttgc acgctttttt ccattatagc aaacttttct tcgtacacga
     9961 cagttaaata aaaatttccg gaaaacttct ttttatttta aataaataaa gccaaaaagg
    10021 agtcacagtc tcgctgcaac tccttttagt ttactatttt gtaaaaactt atgatcgttt
    10081 tccaaagaaa aatgagacaa ctgctaccaa aatcactgcg cctaaaatag atggaataag
    10141 tgccatacct gcagcttttg gtccccattc gccgaaaatc gcttgtccaa tacttgaacc
    10201 gactagtcca gcaatgatat tcgttacaca gcccatcgaa tttcctttac tggtgatcgc
    10261 acctgcaatt gctccgatca cggctccaac aatcaatgtc cataaccaac tcataatata
    10321 acctccttct ttgctaatca attttagcat atgggattta aaggggacaa ataattggtt
    10381 ttgctatttc atattttctt ctgcaaagta aaaaaataaa gaaaacctcc tcttagaagt
    10441 tttctttatt ttttacttgc gtttaaacat actacgaata ccacgtttca ctacatcaaa
    10501 gaaactcaat gattgtacca ttctatcttc atctacatat tctctgatcg cacgaagcac
    10561 ttttttaggt tctttggaag aaaatgtgta tgtaccattt tttttcgttt gaatggcata
    10621 tcgaggaatc cattttccct ttagcatgac tgatgctagt acataatcca cttcttccca
    10681 tggaatttgg atatatttgc gtctatctcg cgcatggtaa aactcaaaac cattattacc
    10741 tatcatgatt tgcccgtaat ctgtcagacc cgtaaacgaa gtagcatcaa ttaccaagtc
    10801 tacttttgta ttgattgatt gaaccatttt ctccctccag atatttgttt atgaacagta
    10861 tactatataa aaaaagctcg ggacaacgcc cgagctatat ttttatggat tataaaagtc
    10921 cgatgacgtg tccaacaaca ccgactacga ataaaccaag gatgataacg attggagaaa
    10981 cctttttctt aagcaaccac atacataaca atgttaatcc taatgcagct aaaccaggaa
    11041 tcaattggtc taagttgtct tgtaatgttg ttaccttttc aggagaaagg gctaagccat
    11101 tattaacttg ttcaaatgca ctcttgattc cttcaccacc tgaaggcaat ttatcccatt
    11161 cgatgtaagc acctttatct aattttacag ttgatacaac tggtaagaat ttgattgata
    11221 cccatctttg tacaagggca gctaatacga acatccctaa aattgaagca ccttttgtta
    11281 cgtcttgtaa caatccgcca gaaaggtcat cagtaatttt agaaccagct ttgtagccaa
    11341 attcttgtgt ataccacatg aatgcccaac gaataatgtt ccaacctaag aagaagatga
    11401 ttggtccaag gatatttcct cccatagcta atgaagcacc tagtgcacct agcattggac
    11461 gtactgtaaa ccagaacact gggtcaccaa caccagctaa aggacccatc atacctactt
    11521 taaccccttg aatcgctaca tcatcaacag gcgcgccatt cgcacgttct tcttccagag
    11581 ccaatgtaac ccctaaaatt ggagaagcaa tgtatgggtg tgtattaaag aattccaagt
    11641 gacgttttaa tgctgcagaa cgttcttctt ttgttttgta taattttttg attgctggaa
    11701 tcatagaaaa tgcccagcca ccgttttgca tacgttcgta gttccatgaa ccttgaatga
    11761 aggtagagcg ccacgcaaca gctaaacgat ctttttttga taatttgatt ttttcttctg
    11821 ccattttatt ttcctccttc aagattaata gtcattcaaa atgtcgccta aagggtcacc
    11881 agagttgctt ccgcctccat tatttgatga accacccatt ttagaaaggt tcaagtaaat
    11941 aagagctaat gcaacaccta aagcacctag agcaatcaat gttaattgag aaattgcagc
    12001 tacaacgaaa ccaataacga agaatggcca tacttctttt gtagccatca tgttgattac
    12061 caaagcgtaa cctacagcta cgaccattcc cccaccgata gccataccat ctgttaacca
    12121 tgctggcatt gattcaagga atgattgcac agtttgtgct gggataaata gcaacgctgc
    12181 tgctgggacc gcgatacgaa ccccttgtaa acatacagca atgatgtgcc acatttcaac
    12241 cttacggatg tttccttctt cagcagccgc atccatcaaa tgaacgattg gaacagcgat
    12301 tgtacgtacg atcattgtta agaacaaccc tgcaacagcc aaaggaacgg caatcgcgat
    12361 cgcagaagga acacctttaa caccttgtcc acctaaaact aaaataattg ctgatgctac
    12421 tgatgctaat gcagcatctg gtgctacagc ggcaccgata tttgcccaac ctaaagcgat
    12481 catctgaagc gtaccgccaa gtacgatacc agcttcgagg ttaccggtaa ctaaaccgat
    12541 caatgtacat gccactagtg gttgatgaaa ttgaaattca tcaagaatac cttccatacc
    12601 agctagaaag gcaactaata cgactaaaat tattgaaata atagacatga taagcctcct
    12661 ataaatttta atattattta ttacgctttt gctaattcag cttttgcttt tttcaaaata
    12721 tcatccatgt tatcgcgtga atcattaggt actttacgta catcgaattt cacacctttt
    12781 tgttcaagct tctcaaacgc ttcaacgtct tcttgtccca tagacaatac tttacttaca
    12841 acgactttac cgaccgaatg cgccattgac ccaacattta attctttgat gtctacgcca
    12901 ccttcaacag ctgtcaatac gtcttcagga ttttcaaata aaagcaatgc tttggtattt
    12961 ccaaaacgag ggtcttttgc tacttcgatc attttgctaa taggaataac atttgctttt
    13021 acacctggag gagcagcttg ttcgattagt ctcttacgca gatcatcctt tgatactgca
    13081 tctgaaacaa cgataatacg gtttggttgc actgcttttg tccaagctgt cgcaacttgt
    13141 ccatgtaaca gacgtgagtc aacacgtgct aatacatatt tgatttttcc gtctccgacc
    13201 acagttcctt ctggaagtgc tcctttaggt tgtgcgtctt caactgctgc tttcggtgct
    13261 tctgccggtt ctaattcttc cggttttact tttacaccgt cttttgctgt ttcaatgatg
    13321 tgtgctgcaa tttcatgagc agattccatt gaaaagcgtg atgcgtaggc ttcaatcaac
    13381 attggtaaat tcaaaccgct gacgattgcc catttatctt tgtgctcttc gaataatgtg
    13441 tttgattgat tgaacggtgt tccgccccat aaatcaacta agaataacac ttcgtcttga
    13501 ttatcaaaag aagcaaccgc ttcttccaat ttggctctca agtcatctgg accttcactt
    13561 ggtttcaaaa tcacggcttt gacgttttct tgttcgccaa agatcattga accggattgt
    13621 aagattcctt cagcaaattg tccatgactt gctaggataa tccctaccat atttatacct
    13681 cctatttact tttgttgtaa caaattcaaa aatattaata attgctaacc attacaacag
    13741 aattatttga ccttttccaa caaccattct tgctacacct ttttgatttt cgctagtagt
    13801 tccatcaaaa ggattttcct gtcagcaggt acttttcgaa tctctaactc cacttcttga
    13861 ctgttcaagt aacgaaagga atcaatatct tgttgattta ctgaaacaaa attggtaatc
    13921 attgatttgc cttcagtaaa actcataccg ccgatattca acgacttcaa ctgtacaccg
    13981 cagcggacaa ccgcttcaac atcttgggga ttggtgaata aaagcatcac ttttgctcgt
    14041 gataataatc cactatgata gatttgaatc attttctgca cggttattac atttgctgtg
    14101 atccccggcg gtgcagcttc ttttagtaga aactttctca atttatcttg agcgactaca
    14161 tcgctgatca caatgatacg ttcaacacct gtttgcttcg accaaactgt tgcgacttgc
    14221 ccgtggatta aacgatcatc tatacgtgcc aatcgaatat ccatctgcaa aactccttct
    14281 tcctcactac ttcacagaaa cccctatcat ttctgtgtga acaaccacct cctagcacac
    14341 actactataa gcaaaaagcg tgccaacttt tttacgatac actaattgaa tttaaaggat
    14401 tcttaaccaa tacacaaaaa cgatacacta taaaagtgta tcgtttttgt gtattcatgg
    14461 cacagtgtat tcttttttat ctaactgatt atggactaga cgtagcagat aatacatttc
    14521 ttctatcggc acttttactt ttatctgatc ttccaacaga acaactgagg ctgccaattg
    14581 gctataaaga ggatcattcg ttgtatttgt caattcgttt tcttccacag tcaatggttc
    14641 attccgaata atacgttcga tcattcctgc tgtgtgcatc actaggttga ttttgaatcc
    14701 atattcttca tcgccgattc ccgtcgtctg agagatttgt gtagaaaatt gccacattgg
    14761 atcgattaac ttactaccat taataaagat gaagttatca gagataaact caacacacgt
    14821 atttttagct gactcttcat ctagggagat gaattcagtt tcctccaatt ctgattccat
    14881 cagcaactga tccaaaatga cttcgatatt ttgatcaata aaacgttcta aaggaataaa
    14941 tggggcttgg atattcaaat cagtaatccc agtagtagct atgatctgat attctttttg
    15001 gataactggc agttcttctt tcacttccac aatcgacaaa gctagaactt ctaattctgt
    15061 ttcttgacgc ttgtttaccg ctcgttcgat caattctttg attctttgtg ctgtaccttc
    15121 gccagatgcg cagatagcca aaacagcttt tttcttatga ataggttttg cttcctgaac
    15181 agattctaca gaagatattt ctgcgtaacc tcgaaaattt ttcaatgatt catgcaacat
    15241 atctagatct gtccctaata ctgagacctt tcgtacagtt tccaaaacta gagaagttgt
    15301 taccatatca acagtacgta ctggaattcc agtgtctctt tgaatttgcg aattaaacga
    15361 cgcaagggac cccatatcta ctaagagtat gaccccgctt ccttcatcta cttcttgtac
    15421 attgcgttca attcggtcca atgctgtttt cggatccata tccaaaggca tgtctactgc
    15481 tcgtacgttc tctgcatcta acaactgttg gacaacttgg accatactgc ttgctgtgct
    15541 gtttccgtga gctgcaacga ctacaccgat tctgccgctt gcttgatttg cccgtaacga
    15601 gacgagtaga acggtcaagt aatagtcttc actttctgga atctccactt gaaaatacgt
    15661 ttcgatataa ctacgcactt ctttcgcaac agcatattca tctggaaaat caaaagccat
    15721 ctcacggatg ttatcattcg tatgccgttc ttctcctaag ttgatttttt tcaaaaaaga
    15781 actgatatgc aagctcatag cataaataaa gttttgctga aactcatatg gtaaagatct
    15841 tttgaccatt acataaatct gattggtcac ttctatgatt ttcggatcta caaattctgc
    15901 taatttgctg tctgcattaa aagaaaaacc atggtcttta tagaaagact tcagatggat
    15961 gttgatatct gtagaaatga aatgattgat tgcttcttga tccaaaccat ccgacttcag
    16021 taaagctgct ttatctccga tgatatcata tagattataa ggtaactcat aggagtccga
    16081 ttggattttc atgatcgtat catttggtgc tacagttatc tttggctcca atattttaga
    16141 aagctctgcc attgcactgc ggttgcttgc taattgaatc aatccgctac gaatcccttc
    16201 tgtcaaatca tcgattgtga tcgagatttc gggtgattgc atatgattta gaaatcctcg
    16261 tgcacatact aactggatat ttgacttcaa ttgtcctata tttccatagg tcacactgcc
    16321 gatcaatgct tttgctacat cttctgtcaa agaaatcttt cgctgcgtgc ggtttgcttc
    16381 gtgggcaatc atgattttta ctaaatcgac tttctcattt gcaggacgtt tttcaaaagc
    16441 cggaagtttg atattgattg ggatacgccg aacaaaagtt tctaatagag aagaacctgg
    16501 gtcttctgtg gtcgcaccta cgatccgtac atttgcttca tgggatttcg ttgtttcccc
    16561 aagacgactg taagttccat gatccataaa gtaaaagatc atttcttgac cttcaggcgg
    16621 caaccggtga atctcatcca aaaacagcat accgccatcc gcttgatcga taatccccat
    16681 tttttcttca tctgcgcctg taaaagcacc ttttgcgtac ccaaataaat gactcatcaa
    16741 aagctcaggg ttattggcat aatcagcaca attgaaaaca atcagttccg tttcctcatc
    16801 tattacatga tgcgcttttg caaaatgata catcgcgtgt gcaaaataag tctttcctga
    16861 tccggtaggt cctgtaatca aacaatttaa tcctcttggc ggatacaaaa tcgcagcttt
    16921 tgcctgttcc actgaatttt tcatgctgcc gttagctcca atgatttttg caaagatatc
    16981 cttagtatct atttggagtg tatttttttc ttcagagacc ggcgtttctt tgtaactgac
    17041 tacatgtttt gtaactggct ttacgtgcgt aacgagtgtt tgatatatgt aacgtactgg
    17101 tcgtccgtct gtctttatca gttttccttc gcgcactagc tgattcagat ctttactgct
    17161 atttgttcgc tgaatcccta acacttcagc aatttcctgt gttgttactc cttgatcata
    17221 ttctgccttt gttaaatgag cggttttttc tttgacatac atataaattc ggtcaatgcg
    17281 ttttgccaat ggtatcgctc cttttctata gactattgta cttaggcttc aaaacataat
    17341 actatgtatt tatttctgtt tcgtcaacta tttacctgat tttgttttgt tgataacacg
    17401 cggatagata gaaatgttca atttaaaaga acgaaaaatc ccgatacgat ttttataatg
    17461 gaaacaagaa aaagaacact gtttaccata taattatttc actgatagca aacaatatag
    17521 cttccatcaa ttgaaaacga tagttatcaa aacatcctga ttccaaattg gcaaaaaaat
    17581 attaagtgta aaaagacaaa gtaaacttcg tagcctccac tacaactttt atttttataa
    17641 acaaaaaagg ctgtgacaaa agtcttgtca caacctcttt aatcataaga ttcaattaag
    17701 cttcttgagc tactggataa acagacactt gttttttgtt acggccttta cgttcgaaac
    17761 gtactacgcc atcaactttt gcaaataaag tgtcgtctcc accgataccc acgtttacac
    17821 ctggatagat ttttgtaccg cgttgacggt aaagaattga tccacctgta actgtttgtc
    17881 catcagcacg tttagcacct agacgttttg attctgagtc acggccgtta gatgtagaac
    17941 cgccaccttt tttgtgggca aataattgta gattcataga taacaacatg gtctgcacct
    18001 cctatttatc attaattgtt ttaatttgga tatattctga gttttcagct tctatcgatt
    18061 gtaatcctaa taaaagattt tctaatagaa tctcagcaat attgttttgt tcttgtgtca
    18121 ttcctgaagt gacttcggta taaagataac cgccttcttc ttcattcatt tcaacaatcg
    18181 gctcaaaacc ggctaatgag gcaatcccgt tgacagtgct gatcgctaac gcagaaacac
    18241 ccgcacaaac gatatcactt ccataaggac cagcatcagc atgcccggtc aaagtgaatg
    18301 aaacgatctg acctgcgtca tttcgcttaa atgttccttt gatcatatat aggccttctt
    18361 tctcaaacgc tattaagcgt tgatcgcgtc gataaccact tttgtatatg gttgacgatg
    18421 accttgttta cggtgtgaat gttttttagg tttgtatttg aaagtaacga ctttcttttg
    18481 tttgccgtgt ttttcaacag taccttcaac agttgcacct gcaacagttg gagcgcctac
    18541 ttttgtagat tctccgccta ctaagataac ttcgtcaaaa acaacttttt ctcctgcttc
    18601 aacgtcaagt ttttcaacat agattgcttg tcccacttca acttttactt gtttaccacc
    18661 agttttaata attgcgtaca tgctataaag cacctccttc tgatatactt agactcgcca
    18721 tccgaagtga cagcatatgc tgtgcttatt acttgttctg agcggttgta gctgtggagt
    18781 ccacaattac aacagtacta gcttaccaga acagcaaccg ttagtcaact aaatttaaaa
    18841 tcaaaaagta aaacaaaaat tcaagcaata cttctgcttt tctatcctca cagttctacc
    18901 aaataaaata gtcgcattgt gtttctttta ttcttataat aaaagaaaaa ggaggagcat
    18961 aaatggaata cccaaaagaa ctgatacaag aagcttcttt aagaatgaac ggccgtcctg
    19021 tagaaggatt tatcgctggg caaggacctc ttcatcctaa actgatgctc gtgggagaag
    19081 ccccagggaa aacagagatt gatacgcatg ttccttttag cggtcaagct ggaaaagaat
    19141 tgatgcaggc actttcttca actggcttaa cacgagaaga ggtgtatatc accagtgctg
    19201 taaggagccg cccgtatcgc gtgactcatc ggatcaataa acggacacaa cagtcagaaa
    19261 ctgtttatcc aaatcggaca cctactcgtt ccgaagtatt tgcgcatgca ccaatattgg
    19321 attacgaact gatgaaggtc cagccaaaat taattgcgac acttggaaac attggtcttc
    19381 aacgtttact tggcaaagaa tatacggtta ccaaaaatca tggtcaactc tatacagggc
    19441 ctgtcttaca attaacagaa caaaaggatg cctacgaatg gtcagaaaaa aactatcgaa
    19501 ttcttccact ttttcatcca gcagctattt tctacaatcg ctcccttgta cctgttatcc
    19561 atcaagactg gcaagtacta gcttcacttt tacagcaata ttaaaaagaa cagacaagac
    19621 cataaaatct tcagtttaca gtcttgtctg ttctttttca ttgttttaat ggctaattgc
    19681 agtatatttc gcatgtaaag ctgtcaacgc atgagcaact gtttgatgtt gatgcgtgaa
    19741 tgtatgttcc cacaagtgag aaagttgttt ttctacttct ggctgcaagg aagtatagat
    19801 aatcgtttgt cctctttgct ctgctttttc ttgaaattca tcaaaaactg cacgtgccgt
    19861 caaatcaata cttggtacgc ctcgcatgga gaaaatgaca ccatcgtgat gatttagttc
    19921 ttccaattta tttttgagtt tttctgaaga cataaaaaac agtggaccac taatatacac
    19981 tactgtccag ttattcagct tttccgtttc tggcagcccc attctttgcc aatcgatcgt
    20041 ttctaatgaa atcgtgatag cagcgctttt gacaacaaaa aatacacagc cgccgatcac
    20101 cccgatcacg atagcaatac ttaaatcaaa gataactgta caaatcatcg taaggataaa
    20161 caatagaact gctgaccaat atttattagt aaatagctcc ttgatcgttt cccattcatt
    20221 catacgccaa gccgtcacaa tcaaaacacc tgcaagtgct ggcattggaa actgtgacat
    20281 cactggcgca aaaacaagca tggagagcag taagacaact gcatgtatca taccagctaa
    20341 tctagtttgt gcaccagatt tgatggccac gcttgtacgg gcgatggccg cagtagcagg
    20401 aatcccgcca aagaaaggaa gcaacagatt tccaattcct tgcgccatta attcttgatt
    20461 gctatctaag ggcttatttg ccattcggcc agccgaagca ccacatagca agctttcaat
    20521 cattcctaat aaagcaatgc taatagctgg aactgccact tgttgcaagg cagaaaagct
    20581 aaaatctccc atattcaaac gattactact gatcaatgtt tgtggaatct tacccacagt
    20641 tgctactggt aaatggaaca atagcataac tgctgttgcc aaaatgatag ccagcagcga
    20701 actaggcatt cgtttcgccc atttttttgg atagataaac atccctaaaa tgactagccc
    20761 tcccatgatc aaagaggctg tgtttatcgg aaatcctatc gtttgataac tacttagttt
    20821 ttcaataacg tttgtacctt ccgagtgaac accaaataaa ttatcgatct gacctaatgc
    20881 aataatgata gcaatacctg aagtaaaccc cgtaattacg ggtgctggga taaaagaagt
    20941 cagtgttcct aatcggaaaa gtcctgctgc aagtaaaaat agacccgcta aaaaagtagc
    21001 aagcaataca ccttgcattc cctttgttgc cgcaatagac atcaagatag ctgccatcgc
    21061 acctgtcgga cctgatattt gataaaaacc gcctgataaa ccgccaataa cgataccggc
    21121 aatgatagcc gtaatcaaac ctgcggccgc atcagctccg cttgataccc caaaagccaa
    21181 ggctaaaggc aatgctacag cagctactgt gatcccagcc aaaagatctt tctgaaatct
    21241 tgaaagatca taacctgaaa attctttttt tagtgaggaa acataatttt tgaacaccta
    21301 tctaactcct ttttcatgaa attttctaaa ataccattca ttagaatatc atatttcatg
    21361 agataaagta atatttttca caaagtttcc tgttagataa attcttattt tttctatatt
    21421 tataagaaaa tagaaagcaa tgccaacaga gctagcccac cttgtttgag taaaatccaa
    21481 cgatcgctag tcaaactgcc atataatgca acaaaaaaaa tagatattaa tagcatactt
    21541 gtgatttctt tagatgcgga agaaagataa gctccataaa gtagtcccac tcctaatagt
    21601 ccattgtaca ctccttggtt cttaaacaat gtttgaacag attttctgct taactcttct
    21661 ttactcattt taaaaaccct acttgtcgtg tcagaatcag ttctcaccgt ttcgatatac
    21721 atgatataaa aaaattcaat tgcaactaga gtcactaaaa ttctagatag tatcgtcata
    21781 tcttcttcct cttttctgat gagttttatt tttttgctcc ttataattgt actcgataag
    21841 gatgtttttt tattttattt gcctttgaaa acaaacaata ttggcacaaa aaacaaagaa
    21901 aaaccgttag aaacctcagt ttctaacggt tttttagcgt cacaggaggg attcgaaccc
    21961 ccgaccgtcc gcttagaagg cggatgctct atccagctga gctactatga caagaatcag
    22021 cggaacatca ataattatag ataattaaat tgcttctgtc aatacgaaat agttatttaa
    22081 ttagcaaata atgaattatt ttggcccaac tactaaatca atcttttaaa acgagacatt
    22141 ctccttcagc tgttctaagg ttatcaattg aaaacaggag ctacattgac ttcatgctac
    22201 tcctgtttat ttagatttcc tgtatccttt aattgactta caaatataga aacttttaca
    22261 tcaagttcga attgagccct cgattcaatc gattatctac aggctgccat aatcatgtat
    22321 cacaactcga ttgtttctga ttcctttgtc attaagatca caccatcacc taatggcaag
    22381 attgtcgatg tcaaatcggg atgatccatc acaacacgaa gaaattcatt taatttacga
    22441 tggatagctc gtttgccacg gggaatctct tcatctggga gcaagatcgt tcctccttgg
    22501 aagatgtcat ctaccatcaa aacgccacct tttcttaaca aacgtaagca ttcaggtaaa
    22561 aattcaatat attttgattt tgcactatcc ataaaaataa agtcatacgg accagacaac
    22621 tccggcaaga tatctgctgc ttgaccttca agcaaagtca ctttatctgt cagtcctaga
    22681 cgttcatagt tttttttagc tttctcgatc atgacatcga aacgatcaat tgtcgtaaca
    22741 tgagcgtttt ctccgattgt cgtggccatg acacttgatg aaaaaccgat agctgctccg
    22801 atctccaaga ttcgttcagg ctgaagctgt ccaagtaaaa atttcaaaaa tacaactgtt
    22861 tcatggggaa taactggtac ttctgctaca tgagcttctt cttccagttt gcctagctca
    22921 cccggtaatt ttttttgctg ggtacgcata aaatccaata gttcttcttt gactacagga
    22981 cgatgcatca tttcattgcg catactactc ctcctcttca cacattatac gaacaactga
    23041 ctaaatctac aagctgcatg agtgttttct cgtgtttcat gttcagaaga gtcgttggat
    23101 ctctggaata catttatgtt tttaacattc ttacaaaaat ttctgtgttc gtctccgctt
    23161 ctttttcaac tctacaaagg tattccatgc tgtgattcct gttaggcaaa aagcaatcgt
    23221 aaagaataac ccctgaatcc atgcaaatgc cgtttttgaa aggactagtc caatatatgg
    23281 aatctgaaag atgggactac cgataatatt tctctttttt actactccag gctcaatata
    23341 agtctttgta tcagcttttg tataaaaatc ttcgtggttg ttttcaacgg ctattacgcg
    23401 acgcgtcgag attttttttc ctgaattttc ataatatgta ataatatctc cgactaaaat
    23461 ctcatttgta tttttcggtc gtacatatat cagatttcct tgtgcataat atggactcat
    23521 ttctttgtcc tcaataatat aaggagtcaa accaaaaatt cggggaacca agacgacgag
    23581 aagaataaga atta
//
LOCUS       DAYYME010000032        23373 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000032 DAYYME010000000
VERSION     DAYYME010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.032"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            9..1670
                     /locus_tag="ACHBFW_001048"
     CDS             9..1670
                     /locus_tag="ACHBFW_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9317372.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1740..2102)
                     /locus_tag="ACHBFW_001049"
     CDS             complement(1740..2102)
                     /locus_tag="ACHBFW_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFM9317373.1"
                     /translation="MKEQSNEKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFL
                     WSLPFLIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLII
                     AGILIALSAIFMLFSLLG"
     gene            2170..3111
                     /gene="opp3b"
                     /locus_tag="ACHBFW_001050"
     CDS             2170..3111
                     /gene="opp3b"
                     /locus_tag="ACHBFW_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM9317374.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3112..4155
                     /locus_tag="ACHBFW_001051"
     CDS             3112..4155
                     /locus_tag="ACHBFW_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9317375.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4161..5231
                     /locus_tag="ACHBFW_001052"
     CDS             4161..5231
                     /locus_tag="ACHBFW_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9317376.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5221..6174
                     /locus_tag="ACHBFW_001053"
     CDS             5221..6174
                     /locus_tag="ACHBFW_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9317377.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6186..7196
                     /locus_tag="ACHBFW_001054"
     CDS             6186..7196
                     /locus_tag="ACHBFW_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM9317378.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7481..8302)
                     /locus_tag="ACHBFW_001055"
     CDS             complement(7481..8302)
                     /locus_tag="ACHBFW_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM9317379.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8399..8677)
                     /locus_tag="ACHBFW_001056"
     CDS             complement(8399..8677)
                     /locus_tag="ACHBFW_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317380.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8945..10954
                     /gene="metG"
                     /locus_tag="ACHBFW_001057"
     CDS             8945..10954
                     /gene="metG"
                     /locus_tag="ACHBFW_001057"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFM9317381.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11433..12020)
                     /locus_tag="ACHBFW_001058"
     CDS             complement(11433..12020)
                     /locus_tag="ACHBFW_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317382.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12195..13517
                     /locus_tag="ACHBFW_001059"
     CDS             12195..13517
                     /locus_tag="ACHBFW_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM9317383.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13672..14448
                     /locus_tag="ACHBFW_001060"
     CDS             13672..14448
                     /locus_tag="ACHBFW_001060"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM9317384.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14441..15010
                     /gene="rnmV"
                     /locus_tag="ACHBFW_001061"
     CDS             14441..15010
                     /gene="rnmV"
                     /locus_tag="ACHBFW_001061"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFM9317385.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15003..15887
                     /gene="rsmA"
                     /locus_tag="ACHBFW_001062"
     CDS             15003..15887
                     /gene="rsmA"
                     /locus_tag="ACHBFW_001062"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFM9317386.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16338..16967
                     /locus_tag="ACHBFW_001063"
     CDS             16338..16967
                     /locus_tag="ACHBFW_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317387.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17091..18200)
                     /locus_tag="ACHBFW_001064"
     CDS             complement(17091..18200)
                     /locus_tag="ACHBFW_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9317388.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18409..18831
                     /gene="mgsA"
                     /locus_tag="ACHBFW_001065"
     CDS             18409..18831
                     /gene="mgsA"
                     /locus_tag="ACHBFW_001065"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFM9317389.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19018..19116
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19154..19690
                     /locus_tag="ACHBFW_001066"
     CDS             19154..19690
                     /locus_tag="ACHBFW_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM9317390.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19730..21493)
                     /locus_tag="ACHBFW_001067"
     CDS             complement(19730..21493)
                     /locus_tag="ACHBFW_001067"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9317391.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21496..23211)
                     /locus_tag="ACHBFW_001068"
     CDS             complement(21496..23211)
                     /locus_tag="ACHBFW_001068"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9317392.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7522 a         4285 c         4761 g         6805 t
ORIGIN      
        1 tacgtaaaat gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg
       61 ctggatgtta tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag
      121 atggaggagg cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag
      181 ttgcaacaga tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc
      241 ttgatgaaga cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag
      301 acggattaac ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg
      361 ttacagcagc ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg
      421 aatatgctta cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag
      481 ataaatcaga actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga
      541 aacaaacacc atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat
      601 ccgtagtgga agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg
      661 gaccattcac attagaagat tttgatggac caggaacaga tacagaatgg acttataaga
      721 aaaatgatca ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag
      781 taaaagaatc ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct
      841 tgtctggtga gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag
      901 cgcggacaag ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga
      961 acctaagaaa agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag
     1021 gtgacggatc agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa
     1081 cgaacgaaga ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa
     1141 aagaatattg ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga
     1201 tggcttctga tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag
     1261 aaaacttgga tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc
     1321 gctcaacttc gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc
     1381 caagcagctt tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca
     1441 atgcagaata tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag
     1501 cccgttggga tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc
     1561 cggtgtacca aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc
     1621 atgcggcagg cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac
     1681 cgtgagcatg agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact
     1741 tatcctaaca aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg
     1801 atcagccaaa agctgtacat accgtagctg gcagacgaga gcgtggaaaa ttctggtttt
     1861 ttctttcggt tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc
     1921 gaagaaaaga cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga
     1981 tcattagata attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc
     2041 agaatcagta gaaaagctac tgcgtagggg atagattttt tttcatttga ttgctccttt
     2101 atttttggtt cattatctaa taatgtagcg aaacatctgt cagaagtcaa aagctagggg
     2161 gaaacattag tgaatagtta tatcaaatat gtattaaaac gtgtcttttt catgatcatc
     2221 acgctttggt taattgcaac aatcaccttt ttcttgatgc aattgctgcc gggcacgcca
     2281 tatacgaatc aggaacgttt gagtcctgaa acaatcgaga tgctaaataa acaagtcggt
     2341 ttggataaac cagttatcgt acaatatggg atttatcttt ctaatttgct tcaaggagat
     2401 tttggaattt ccttccaatt taaaaaccag ccagtcgcac atttgttagc tggaagaatc
     2461 ggaccatcct tgcagttagg gttacaggca attatctttg gaacagtcct agggacgatt
     2521 ttaggaacga tctctgccat gaagcaaaat acatgggcag atacatcttc tacactcgta
     2581 gcgatattag gtcgttccat tccaaacttt gtatttgctg ttttattaca atatatcttt
     2641 gcaatcaaat tacaagtttt gccaatcgca aaatgggatg gctttgtgta cacaatctta
     2701 ccaacaattg cgttggccat gtcgccgctt gctgactctg ctcggtttat ccgtacggaa
     2761 atggtggaag tactgcatag tgattatgtg gaattagcaa aagcaaaagg attgagccgt
     2821 tgggaaatcg cctttaaaca tggtcttcga aatagtttga ttccattgat gactttatta
     2881 ggaccgttag ctgtagcatt gatgaccgga tcactggtgg tagaaaatat ctttgctatt
     2941 cctggtatcg gggaacaatt cgttaagtca atcacaacaa atgattatcc tacgatcatg
     3001 gctgtaacga tcctttactc atttatgctg atttttgtta ttttgatcgt ggatctattg
     3061 tacggtctag ttgatccaag aattcgtgtt tcagaaggga gccgaggata aatggaaatg
     3121 ataccaaagc gttatgaaac agttgcagat atccctgcgg atgaatttct tccattacaa
     3181 aaaaataccg aagaagaacg tgaacagatt actgcacctt ctttgaattt tatccaagat
     3241 tcatggcgcc gtttaaagaa aaacaaagct gccgttattt caatggtttt actattggtc
     3301 atcattttga tctctgtgat cacaatcttt gtttctcctc atgatccaac tgcccaaaac
     3361 gtggcttata tcaatttgcc tcctcgtatt ccgggaatca atatcgatgg attgaatgga
     3421 aaagcaatgg ttggtggtga acttgttgat aaatatgctc aagcaaacgt cccttcaaac
     3481 gtaaatttct ttttaggaac agacggtttg ggacgtgatg tattaagccg cttatttatg
     3541 ggaatgagaa tctcgttgct gatcgcattt attgctgcat tactagatgt aacgattggt
     3601 gtagcttacg ggttggtttc tggtttgtta ggcggacgtg tcgataacgt gatgcaacgt
     3661 tttttggaaa tcttatcagg aattccaaac ctcgttgtca tgattttgat gttggtcgtt
     3721 tttgaaccag gaattttttc aatcgtggct gctatggcca tcacaaactg gataccaatg
     3781 gcacgtatcg ttcgtgcaca aacaatgaaa ttaaaagatc aagaatatgt tttagctgga
     3841 ttgacattag gtgaatcaag aatgaaaatt gcattcaaac atattttgcc gaatatctct
     3901 agtgtcatta ttattcaaat gatgtttagt attccatctg ctattttctt cgaagccttt
     3961 ttaagcttta tcggtttagg tcttacgcct ccgtcagctt cattaggaac gatgttgagc
     4021 gaagggtaca agacattcct gtatctgcct tatctattat ggattccggc agtaacttta
     4081 tcggttatca tgattggctt taacttactt gctgatggat tgcgtgatgc ctttgatcct
     4141 aaaatgaaag agtgaagacg atgacgaaaa ttttagaagt gaaagactta gaaatctctt
     4201 ttgatacgta tgctggaaag gtacgagcga ttcgcggtgt caattttcat ttgaataaag
     4261 gagaaacatt ggcgatcgtt ggggaatctg gaagtggaaa atcagtgacg accagaagca
     4321 tcatgcgctt gctgtcaagc aacgccaata tagatgctgg tcaaatcctc tttaaaggta
     4381 acgatatcgt tggaaaatca gaaagagaga tgcaaaaaat ccgtggtaaa gaaatagcga
     4441 tgattttcca agatccaatg acctctttag atccgacgat gcccattggg aagcaagtcg
     4501 ctgagtccct tagaaagcat aataaagttt caaaaaaaga gggacaaaaa gctgcattag
     4561 aattgcttaa attagttgga atacccgaag ctgagaaacg tatcaacagt tatccgcatc
     4621 agttctcagg aggtcagcgt caacggatcg tgatcgcgat tgcactggta tgttatccgg
     4681 aaatcctgat tgctgatgaa cctacaacag cgcttgatgt gactattcaa gcacagattt
     4741 tggagctgct gaaagaaatt cagacaaaaa tcgatacatc aattatcttt atcactcatg
     4801 accttggtgt agtagcgaat gtcgctgatc gtgtcgctgt catgtatgga ggaagaatcg
     4861 ttgaagttgg aacatcggaa gaaattttct acaacccgca acatccctat acttggggac
     4921 tgcttggttc aatgccgaca ttagatagtg cgaacgatcg attgtatgct attcctggtt
     4981 ctccaccgga tttattgaac ccgccaaaag gagacgcatt ctatccaaga aatgaattcg
     5041 cgatgaaaat cgatgcagaa caagaaccac cattttttga actatcaaaa acacacaagg
     5101 cagcaacttg gctacttgct ccgcaggcac ccaaagtgac accgccagaa gaaatccaac
     5161 gtcgctgggc tatctttaaa gagaaacaaa aacatactta cggtcaagga ggaattgctg
     5221 atgccaaata aactacttga agttaaagga ttgaaacagt atttcaatgt aggaaaaaag
     5281 aatgaagtac atgctgtaaa tgacatcagc tttcacattt atgaaggaga aacattcggt
     5341 ttagttggag aatctggaag cggaaaatcc actactggcc gtacgatcat tcgcttgaat
     5401 gaacctactg atggtgagat attattcgac ggtcaagata taacgaagat caaagataaa
     5461 aaaggtttga cgaaattccg gcatgatgtt cagatgatct ttcaagatcc atacgcttcg
     5521 ttgaatcctc gtatgaaagt cagagatatc attgccgaag gaattgatgt taatggacta
     5581 gctaaatcat ctgaagagcg tgcaaaaaaa gtcgatgatc tattgagaac tgttggattg
     5641 aatccaagcc acggtactcg ttatcctcat gaattttcag gtgggcaacg ccagcgtatt
     5701 ggtattgcac gagcattggc agtaaaaccg cgttttatca tttgtgatga accaatttct
     5761 gcactagatg tgtcgatcca agcccaagtg gtcaatttgt tgcaagatct ccaaaaagaa
     5821 catcaattga cctatctatt tattgcacat gatttgtcga tggttaaaca tattagtgac
     5881 cgaatcggtg taatgcataa cggcttgtta ttagagatgg gaacaagtga tgaaatctat
     5941 aatcacggtg ttcatcctta tacagaaagt ttgttatctg ctattcctct accagatcca
     6001 gaccatgaga gaaaacgcag aagaatcaaa tatcaacctg aaccagatga cggacaagta
     6061 cgtcaacttc gtgaaatcgc accagaacat tttgtctatg caactgagca ggaagtccca
     6121 tattacgcaa aaaaactaaa acgacaaaag gaatctcttc tggttgctaa ctaggaggga
     6181 catagatgct aaaagctttt aaattccgta tgtatccgac agaagaacaa aaacagcaat
     6241 tgattcgtac ttttggatgt gctcgattta cctataacca tttgctgaaa aagcggcaga
     6301 aaagttggca acaaacagga gtagccaatt tttcgctcac acccgctaca ttaaaaaaag
     6361 aatacccatt cttaaaagaa gtagacagtc ttgctttagc aaatgcacag cttaatttgg
     6421 atcgggcttt tcgcaattat ttcaagggac gtgccagctt cccaaagctt aaaacgaaaa
     6481 aaagtatgtg gcaatcttat acgaccaaca accaaacccg tacgatctat ctggaaaatg
     6541 gctacttaaa gttaccaaaa caaaaagaac taatcaagat aaatcagcat cggccagttg
     6601 aaggatcgat tcgctccgct acgatttctg ctcgatataa tgaagaattt tatgtcgcac
     6661 tactttgtga tgtttcgcca gtaaaaaaag aatctttagc taaatggatc gggatcgcct
     6721 atcatccgaa gacattgatc caaacatctc gacctctaga agtaacttta ccaaaatttc
     6781 accagacaga agaaaagctg caacatgcac agcggaaatt gaatgtgaaa gtccgctccg
     6841 ctcaccatag aaagatcagg ttagatcagg caagcaatta tcagaaacaa aagagaaaag
     6901 taatggatct ttatctgaaa caaaagaacc agcgagaaga ttatcttgaa caattatctg
     6961 ggaaattggt caaacaatat gactacttat ttgtagaatc ttttccaaaa gaagaggcac
     7021 atgctgattt ttctattcac gactggcata aattaataac aaaattgcgt tataagtccc
     7081 aatggtataa taaaaaattt cttttgatca ataccgacgg agcagaagaa agcaattccg
     7141 taagaaagag ccaagtagta gaaaaaatgg gcagacattc ggttatcaag ggataaaaaa
     7201 tgaatgaaac tgataggagc gttagggtta gccttgttaa taaaaagtct taatgacttt
     7261 tgtttctagg aatttttctt ttgaagaaaa gctcactaat tatgacaaac caataaaaca
     7321 atagcagaaa aagttcgtac gagctttttc tgctattgtt aattttttat tcagaaagtt
     7381 tacgaataca tcaaaattct gaaacaaaaa acgtaactca caaagagttg tttttgcgtt
     7441 ttttcttgaa tgattaggtc tgaatctttt gctcacaacc ttattcatgg tgccccggct
     7501 tgagctgata gatttttgaa ggacggccga ttcctacagg acgttcaccg atctcatcga
     7561 aatattggag catagctttc ttaaaattgg aatgatcgat cgttttataa tcgatcccta
     7621 agaatttagc aaatactttt cgagcttccg taatcgtgaa atctttacct aaaacttgta
     7681 gaacttgcgg ctcgtgttcc attttattga ctacacggtt gaatgctttc aagatgattt
     7741 gactatggtc aaaagccaaa gtatctttgc cagaagactc tccagtgact aaatcaagcg
     7801 agatagccac ttctccgcta gataaatgga tcttgttact atgacgttct agtgtgaacc
     7861 agcgtacttc cttggcatca tctccggcga ttagcggttc ttctccaata aaggctaagt
     7921 agctgactgt gacgacccag cctcgcggat ctcgattagg cgtactgaat gtatgcagtt
     7981 gctcaatatt ttcttttgaa ataaccacac cagtttcttc ctttgtttct cttaagacgc
     8041 tttctccagt tgactcatcc ttttggacaa agccaccagg caatgcccat gaatttcgat
     8101 aaggatgccc ttttctttgg atcaacaaaa ctttcagctg atcttcttct ttgttatagc
     8161 acatcagtac gatatcgaca gtcaaagagg gcttgtcata ttctggacgt tcctgttttt
     8221 gataccagtt aagaaattct tcttcacttg ctttatgttc ataatataat ttttcttctt
     8281 ctttagaagc aaaacggttc atctgaccac ctccattgac agtaaaagta ctttttacct
     8341 tattgtagca gactctgaaa aaaaaccaag gatttacttg aaaaatctag gttatcattt
     8401 aaatatgaaa aataaatgga agtatgagat tgaataaaaa gaaaagcaga taaaacggca
     8461 atactttata ccgctcttca cgaatggcaa agaagccaca aataacgata tatacgtcaa
     8521 tgataaaacg caaaatccaa gctgctgtca taaaactgtt caaatgccca tggaggttaa
     8581 aataaccata catacgaata taaaaatagc tgaatattaa atgtgctaat gaaagaaaag
     8641 caatactaag tgtaaaaaaa agatgtttct tctgcatgat tgttccagtc ccttactaag
     8701 atagatagtc tttattatac cacgtgtgag gggagaaata aaaagccgtt ttttagtaga
     8761 tatgaaacaa aacaaaaacc cgcatttatt caacctattc atccttttaa aggatagatt
     8821 gaacgtagat agaagtcgtt tatccagtct ttttcatttt gaaaaaaagc taacagcatg
     8881 ctttttttat ggtatactag caaggaaacg aattcgcatc atgcgataaa ggaggaagag
     8941 ccccgtggca gaaaaggaaa ctttttatat cactacgcca atctattatc caagcggcaa
     9001 acttcatatc ggcaactcgt atactacgat tgcatgtgac gcaattgctc gttataaacg
     9061 gttgatgggc tttgatgtgt tttatctcac tggagtggat gaacatggtc aaaaaatcga
     9121 aacaaaagca gaagaactag gtgtaaaacc gcaagaatat gtagataaaa tggcggctga
     9181 tgtcaagaaa ttatggaaaa cgcttgatat cagttatgat aaattcattc gcacgacaga
     9241 tgattaccat aaagcagctg ttcaaaaaat atttgatcgt ttattagaac agggagatat
     9301 ttacttaggt gaatatgaag gctggtactc tgtttctgac gaagaattct ttacagaaac
     9361 acaacttgca gaagtgtaca aagatgacga aggtaacgtg atcggtggta aagcgccaag
     9421 tggacacgaa gtagaattgg taaaagaaga atcatacttc ttccgcatga gcaaatacgc
     9481 agaccgtcta ttggaatact atgaagaaca tcccgaattc atccagccag aatctcgtaa
     9541 aaacgaaatg atcaacaact ttatcaaacc aggattagag gatcttgcag tttcacgaac
     9601 aacttttact tggggagtac cagtcaagag tgatccgaaa catgtggtgt atgtatggat
     9661 cgatgcatta tccaattata ttactgcact aggatatggc tccgaagatg aaagtctatt
     9721 cgaaaaatac tggcctgcag atgttcagat ggtcggtaaa gaaatcgttc gctttcatac
     9781 gatctattgg ccaatcatgc tgatggccct tgatttgcct ctacctaaaa aagtatttgg
     9841 gcatggctgg ctattgatga aagacggaaa aatgtctaaa tcaaaaggaa atgtcgttta
     9901 tcctgaaatg cttgtggaac gttatggttt agatgcactc cgttattatt tgcttcgtgc
     9961 tgttccgttt gggtcagatg gtgtctttac gccagaagat tttgtttccc gtgtcaatta
    10021 tgatctggca aatgatctag ggaacttatt gaatcgaacg attgctatga tcaacaaata
    10081 ttgtgatgga ctcgttcctg actatgcctc acttgtcact ccatttgata gtgaactgtc
    10141 tacgacggct gctaacgttg tcggacgtta ccatgatgca atggaaaaaa tggaattcaa
    10201 tacagcattg gctgaaatct gggtgctgat ttctcgtgca aacaaataca ttgatgaaac
    10261 agaaccatgg gtattagcaa aagacgaaga aaagaaagct gaattgaata gtgtcatgat
    10321 ccatttagct gaatctttgc gtatcgcagc gatattactt cagccaatca tgacagaaac
    10381 gccagagaag atcttcaatc aactaggatt ggactctgaa acaatgaatc ttgaaggatt
    10441 acactttggc gaatttcctt caggaacaaa agtagttgca aaaggtacac caatcttccc
    10501 acgtttagat atggaagaag aagttgcttt tattcaagaa aaaatgtctg aaggtactca
    10561 aacaaacgaa gatacagtca aatgggatcc agaagaaaca gaacttgttt caacgaaaga
    10621 aaaacaaatc aaatttgatg tatttgaaaa agtagaacta aaagtagcag aagtcatcaa
    10681 ctgtcaaaaa gtagaagggg cagataagtt gctgcaattc cgactagatg ctggagacag
    10741 ccaagaccgt caaatcttgt caggaatcgc tgaattttat cctgatccaa gcgaattgat
    10801 cgggaaaaaa gtcgtcattg tagcaaatct gaaaccacga aagatgcgtg gacaaatcag
    10861 tcaaggaatg atcctttctg cagaagcacc agatggctct ttacaagtaa tcgaagcacc
    10921 aaaatccatg ccaaatggct cagaaatcgc atagaaattc aaagggggta tgacactagt
    10981 tgtgtcatac cccctttttc cgaaatttga gaagcaattt tcgtaaattc cttcttatta
    11041 cttgaagcca atcatataat acagaaacag agaactatcc aaaataaatc ctagaaaaag
    11101 aagtacgagc ttcgctgatt tttgttaatt tagaataata ctaataggaa tcgtaaaaac
    11161 agaaaaaaag cataatgata aagggtcggg cagattttaa ttgattcatc tggtactctt
    11221 ttttattgtc cttacattca taaagtttga atgcaatgac tttattacat ctatatttat
    11281 atctaacagt agcgttgatg tgttaattat aagttctatc actttatgtt caattagttt
    11341 gtgctcttat atgtgtgatt gaaaatatag ataaaaaaac aatcatcggc tttgacttcg
    11401 atgattgttt tctttatttt gtaaaaatcc tatcacttga ttttatggta accttttatt
    11461 tttgtcttca aacggcttgg taagagagca gtaagcagtg caaatgggat cgaaaagaca
    11521 taccagaaaa tcgtacgtcc gataccataa tgttgataga gctttgtcaa catcacgtgt
    11581 aacaagatca accctaaaag caaggagcca gctataaacc aatgattatc ccaatgcatg
    11641 acttcactaa gtgccatcac tccaaaagga tagaaataaa aatagatccc gactaaggtc
    11701 atccgccagt tctttgtaaa tgttccgata ttcaataaaa agaaaatcgc tgaaagcaaa
    11761 ataccgatag gcggaaaaag cccaaccaaa aaggaataca acatccccca caagatcatc
    11821 acaggacctt tgatcaatgc aacgattgcg atgaacaaag tcagcaatat tagtttttgt
    11881 gataacgttg caacagtcat taatccaaat aaaattaatg tgaaccaatg ccaatgggac
    11941 tgctgttgtt ctttacggga aaaatcgacc cggttggata aataatctgc taatttcgga
    12001 taagaacgct taaaatccat aaactgttcc tccttcgtgg aaaaggtagg tttagtatac
    12061 gaaaaataaa aaagaaatac atccgtctta agagggagtt tttcactgaa agtggagaaa
    12121 aaagaatggt aaattaaaat attctcagtt atactgaaaa ttataccaaa tttcaaagaa
    12181 gaaagaggat gggaatgaaa ggcaaactaa aacgagaaat caatctgttt ggcgcattag
    12241 ctaccgttat gggaaccgtg atcggtgcag gcgtcttctt caaaactgct gctgttactg
    12301 ctagtactca gtctacaagt ttgacattgc tagcttggct tttgggtggt ttcttaacga
    12361 tctgtgctgg attgacagtg gctgaattag ctacagcaat cccggaaact ggtggagctg
    12421 taaaatatat tgaagctgcg tatggaaaat tgcccagctt tttattagga tgggcgcaaa
    12481 gcttgattta ctttcctgct aatattgctg cattatcgat tatctttgcg acacaattga
    12541 cgaacttatt acaattatct accgattatc ttttgttgat tgcagtgatc accgctgttt
    12601 ctgtgacagg gttgaatttg ttagggacaa aagtcggtgc ttctgtacaa tcagtaacgt
    12661 tgattgttaa attaatacca attgctgtga ttgtcatatg gggattattg accccaggac
    12721 aaggaactgt ccagttattt ccaatcgaag ctggaaaaga tgttactttt gtcgaaggat
    12781 tgagcagtgc gttgttagca acgttgtttg cttatgacgg ctggttaggt gtcggtgcaa
    12841 tggcagggga aatgaaacgg ccggaaaaag atttgcctaa agcaattatt cttggtttga
    12901 gttttgtaac agtcgtttat ctattgatca attttgtttt tttgaaaact ttgccgattg
    12961 atcatcttgc cggtaatttg aatgcagctt ctgaagcatc agacgtcatt tttggaggaa
    13021 tcggtggaaa gctggtcact attgggatct tgatctcggt atacggtgca ttgaacggtt
    13081 atacactaac tgggatccgc gtgccttatg cgatggcttt agaagacgag ctacctttca
    13141 gtaaacagct gacaaagctt tcaaagaagt ttacggttcc ttatgttcct gcggttttcc
    13201 agttagcagt agcttgtatt atgatgagcc ttggttcttt cgacttttta acagacatgt
    13261 tgatttttgt gatgtggcta ttcagtttac tgatttttat cggtgtcttt attttaagaa
    13321 aaaaagcgcc ggaattgcca cgtccatata aagtaccact ttatccaatc gttccgatta
    13381 ttgcgattct cggtgctatt ttcattttag gtatgacgat gatgacgcaa acgaaattag
    13441 cattgatcgg tataggtgtt accttgatag gtatcccagt ctattatcaa aaaaagaaaa
    13501 aacgctcaga agagtaaaat atttcaacaa cagacagaaa aagtacgaac taggtcctct
    13561 agatcttcgt gctttttcta tttcaataaa acaagttctt ctattttgat ttttgctact
    13621 tctgcaagta gtgcttatgt gctaaagtaa gaatgaaaag gagagataaa catgatattt
    13681 gattctcata cccatttaaa tgctgaacag ttcaatgaag atattccaga aacgattcag
    13741 cgagcacaag aaatgggggt aacgaaaatg gcggtcgtgg gcttcgatac gccaacgatt
    13801 gaaaagtctt tgcaattaag ccatgactat ccaaatatat acagtattat cggctggcat
    13861 ccgacagagg ctggcagtta tacgaaagaa atcgaaaata aattacaaga acaattgact
    13921 ttaccaaagg ttgtagcatt aggggaaatt ggcttggatt atcattggat ggaagacccg
    13981 aaagaagtcc aagataaagt atttcgcaga cagattgcta tcgcaaaaga aatgaaccta
    14041 ccaatcagta ttcatactag agaagcactt gaagacacgt atcaaatcct aaaagaagaa
    14101 gatgttcgag atatcggtgg aatcatgcat agtttcagtg gtgattatga atgggcaaaa
    14161 cgatttcttg acttagggat gcatatttca tttagcggtg tcgtcacttt caaaaaagca
    14221 ttagacgtcc aagaggctgc agcaaatgta ccaatcgaac gattacttgt ggaaacagat
    14281 gccccttatc tggcaccagt tccttttaga ggaaagcgaa atgaaccagg ctatactcgt
    14341 tatacagtag aaaagattgc tgaacttcgg aatcttccga cagaagaagt ggctgcccaa
    14401 acatggacaa atgcgcaccg attatttggt ctaagcgaac atggataaga tgaagatcca
    14461 agagattata gtcgttgaag gaaaagacga tacaagaagg ctgcgggaag tagttgatgc
    14521 agatacgata gaaacaatag ggtctgcaat caatgaagag atcctgatgc aaattgaaca
    14581 tgcccaagag actcgtggag tgatcgtttt taccgatcct gatttttcag gagaaaaaat
    14641 aagaaaaatc attatggaag ctgttcctca ggcaaaacat gcttttttgc cgcgcacaca
    14701 agctaaacca aagaaaaagg gaagccttgg tgtcgagcat gcaagtgatg ctgctatttt
    14761 agaagcattg aaaaaggtcg tgacgccaga catttcccaa acggctgttt ctgaaatcac
    14821 tagacaagac cttttagact atggtttaat agctggggca caagcaaaag aaaaacgaga
    14881 aaaactaggt gacgagttgc gcatcggtta cacgaacggc aaacaattgg aaaaacgttt
    14941 gaagatgttt cgtattacgc cgaaagaact cgcccaagca atgaaaatag tggaggaaca
    15001 acatgagtga atatcgtgaa attgcgacac cctctagaac aaaagagatt ttattgaaac
    15061 atggtttttc atttaaaaaa agtttaggac aaaattttct gactgaacca aatattttaa
    15121 ggaaaatcgt agaaactgcg caaatagatg aacatacgaa cgtgatcgaa gttggaccag
    15181 gaatcggtgc attgacggaa caattagcca agcatgcaaa acaagtcgta gcatttgaga
    15241 tcgatgatcg tttgatccct gtattggctg atacgatgca accctatgac aatgtgacta
    15301 ttgtacatca agatatttta aagaccgatt tgtcaacagc tgttagagaa acatttcatg
    15361 aggagcttcc gttaaaagtc gtagcgaatt taccttacta tattacgaca ccgatcatga
    15421 tgcattttct tgaatcagat ttggttgtgg atgaactagt cgtcatgatg caaaaagaag
    15481 tagctgatcg tatctctgct gaaccaggaa cgaaagctta tggttcatta tcgattgctg
    15541 ttcaatatta tatggaagcc agtttagcat tcatcgtacc aaaaacagta tttgtccctc
    15601 agccaaatgt agattcagcg attttgaaac tgacaagaag agatataccg gcagtcgaag
    15661 tgacagacga aaaagcattt ttccgattga caaaagcggc atttcagcaa agaagaaaaa
    15721 cgctttggaa taacttgcag cattcgtatg gcaaagatga ccagacaaaa gcatggttag
    15781 caaaaagctt ggaaacagct ggaatcgatc caaaacgtcg tggggagact ttgtcattac
    15841 aagaattcgc cgcattaagc aatgcgatgt cagaaaatat gcagtaaaaa aagaggttgt
    15901 gacagaagtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa acaaaaatag
    15961 ttcctcatgt ttttcgtttg tttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16021 ttaagaaaga ggttgtgggc ctgccgttta actctaagtc acaaggaaca atttcaaaaa
    16081 acagcttctc atgtttttga gttagttggt cttattactt gatgcaagct tttgaccact
    16141 attatgccga acaaggagat gtgacacaaa ttttgtcaca tctcctattt ttgtttcttg
    16201 atcaaaaaat tcttatactt gatactcaca ctcattttgt atggatttag aaaagaatat
    16261 gtgtcttgac agttattagt aagtgagtta tactaagtcg acatttatgt gtcaagacat
    16321 attaggagag agaaaaaatg agaaaaaatt cagttcgaaa actaacgatt tctgctttat
    16381 tgattgggat gggggtcatt atcccaatgg tcatgcctaa aatcatgatt ccaccagctt
    16441 cctttacttt ggcgtctcat gtgccattat ttattgcgat gttttttaca ccaggagttg
    16501 cagtggctgt tgcgctaggt acgacatttg gtttcttttt aacaacgccg gttatcatcg
    16561 cattgcgagc attgtcacat cttgtgtttg cgttgattgg ggcttggtac ttgcaaaagc
    16621 atccgagtat cgttttaaaa aatggacagt ttacttttgc taattggcgt ttccaagggt
    16681 tcaattttgt gatcggtgtg attcattctt tagcagaaat gctagtagtc agcatctttt
    16741 actttattgg taatatgcct gaaacatatt attctcaagg atatttttat acagtattca
    16801 tcttgatggg gattggtggg cttatccaca gtttagttga ttataatatt gcttatttta
    16861 tagcaggaac actcagcaaa tattttgata tccccgtatt tacggcagca aaaaaagaaa
    16921 atcggaaaga gtcgattcaa gggtacgcga agaaaacgac atattagaca tgatctctca
    16981 aaaaaaaaac tctcatctag taatggataa taaatcctct aagatgagag ttttttgtcc
    17041 taaaaagagc tgcaccaaca tcttctggca cagctctaac agggggcaat ttatggaagt
    17101 ttgatgactt gttctgtttc tttatcaaag aagtggcctt tgctgatgtt gaaggcaagg
    17161 tcgactattt cgcctggttt atggaaatct cttgcatcta cttttgagat gaactcagta
    17221 gaaccagttt ttgtgtaaag catcgtttca gctccaagta attcggaaac gacaacttct
    17281 gatttcacta cggcttcagg tgaagcttca agggctactt gttcactgtg gatgtcttct
    17341 ggacgaatac cgaagataac ttcttttcct tcataacctt tttcaactaa tactttgttt
    17401 tttccttcag gaatacgaag cttaagccca tgtccatctg tgataacacc atttttcaat
    17461 gtcacattaa agaagttcat ggcaggagat ccaataaagc cggcaacgaa catattgacg
    17521 ggtgtatcat aaacttcttt cggtgtaccg atttgttgga taaatccgtc tttcatgata
    17581 acgattcggt cagccatagt cattgcttct gtctgatcgt gagtaacata gatggtcgtt
    17641 gtttcaagac gctggtgaag tttggcaatc tcagcacgca tggctacacg taatttagca
    17701 tctaaatttg ataatggctc atccattaag aagacttttg catcccgaac gattgcccgt
    17761 cccaatgcca cacgttggcg ttgtccacca gataaagcag ctggttttcg tttcaagtat
    17821 tctgtcagac ctaagatctc tgctgcattg tccacacgtt ttttgatgtc tgctttatca
    17881 tatttacgta atttcaagcc aaaagccata ttatcaaaaa cagtcatatg aggatacaat
    17941 gcgtagtttt ggaaaaccat ggcaatatca cggtctttag gtgccacgtc attcatgact
    18001 tttccgccga tatttagttc tccttcagag atatcttcca gtcccgcgat cattcgcaaa
    18061 gtagttgact ttccacaacc ggaaggacca acaaaaacga taaattcacg gtctttgatt
    18121 gatagattaa aatcagttac agaataattt tctgcattgt catatttttt atatacgttt
    18181 ttcagagcca tttctaccat aatacgttcc acactttctt ttttagtttt attaactatt
    18241 taaagtataa atgaaagcgt taactaactt caatgtcatg ttgaacaaga attttttctt
    18301 ttttttgtca atgtgctagt tttaagcttt aataaatatc cataagaaat aaaaactgat
    18361 acactggagt aaataagtat gttaacaata tcaaaaggag tgtctcttat gaaaatcgct
    18421 ttgatcgctc atgaccgaaa aaaacctatg atggtgaaat tagcaacagc gtataaaaca
    18481 atattggaaa aacatgaact ttttgctacc gggacgactg ggcagaggat cacggaagca
    18541 accggcttgc cgatccatcg gtttaaatcc ggaccgttag gcggggatca gcaaattgga
    18601 gccatgattt cggaggatca gttagatttg gtgatttttc ttcgagaccc gctggctgca
    18661 cagccccatg aaccagatgt aacagcactt atccgtttat ctgatgtcta tgagattccg
    18721 ttagctacga atatcggaac agctgaggtg ctactaagag gactggaagc cggcttccta
    18781 gattttcgaa gaatcgtcca tgaaatagac aagcatccac tttcattttg atggataagc
    18841 gtatggaaaa ataacgttta atgttcgcct atatgtagct ataaaagcta aacaaactga
    18901 taatatgcga tataatacga acgtattgga taatataaac aataatatta gtttttcgta
    18961 tttgcagtaa atgtgaaaat atgctatagt ttctgtggga aaagatccca aaaataatac
    19021 agagtagaaa ataaagcgtt aagtgctaag aggatgggat gttgcctctt ggacgaagga
    19081 cgtaatgttc gcggttttat tttcgcattc gctgcatgat acgatgtagc agctcttaaa
    19141 ggagatgcta aaaatgaaga aacaccggtt agacgcacgt atgattgcta ttatgggctt
    19201 attgattgca ttgatggtca ctttgtcccg actggtagca atcgagaccc catttattaa
    19261 aatcagtgtc acatttattc cacaagtgat catgggcatt ctttttggtc ctttttggtc
    19321 agggataggc gcagttttag ctgatttagt aggaatggcc ctcttctcga aatctgcatt
    19381 ctttattgga tttacattga atgcatttat tgaaggagca atctacggat tctttttcta
    19441 tcgaaaagag atcacttgga aaaatgccat tctggctacg cttagtgtca cgctgatcat
    19501 caacttattt ttgacaccat tatggcttgc gttgatgtac catgtgccat tgttcagctg
    19561 ggtcgtttgg gcgccccgcc tgttaaaaac agtaatatgg ttaccgattc aatcaattgc
    19621 catttattac gtaggacgta gtattcctta caaaaaaatt ttaagaagtt tagcgatcca
    19681 cgctaaataa aaaacaagca gtcttcccat cccagaagac tgcttgtttt tattgtgctg
    19741 tgaattgtgc ttgatagagc cgtcggtaat aaccatcttt ttgaagcagc tcatcatggg
    19801 tgcccatttc gacaatagct ccctgatcca tcactaatat ctggtccgca tttttgattg
    19861 tggacaggcg atgggcaatc acaaaactgg tttttccttc catcattgct aagaaggctt
    19921 gttggatttt tttctctgtc aatgtatcga ctgagctggt agcttcatct aagatcaaca
    19981 tagggggatt actgatcatc gttcgagcaa tcgtcaacag ttggcgttgt ccatctgata
    20041 tctttaaccc ttgaccgcca atcttagtat ctaatttttc cggcaaacgt tctacaaatt
    20101 catacatata agaggctttt agggcatcat tgatttctgc gtcacttgct tccggattcc
    20161 caaaacgaag attttctcgc aatgtactgt caaatagcca agtatcttgt aacaccattc
    20221 caaaactttt acgtaatgta tcacgttgga tagagcggat gtcatggtca tcgactgtaa
    20281 ttgttccttt tgttgtgtca taaaaacgca tcagcaaatt aacaagtgtg gattttccgg
    20341 cccctgtttt tcctactata gcaatggttt gacctggata agcagtaaaa ttgaaatctt
    20401 cgatcaaacg ctgcttgggg tcataagaaa aagaaacatg atcaaaagag acttttcctt
    20461 tgatattttt gggttccaag gcataggcat ctggcaaatc aggcttttct tctggctgat
    20521 cgatcaaatc aaatgctcgt tctagaccag aaaaagcggt ttgcagttgt gtcgtgattc
    20581 cagaaagttc gataaatggt ttagaaaact gacttgagta gatcgtaaag ctggaaatca
    20641 caccaacagt gattgacgaa ccactcttca atgccaatag gccgccaact aaaccaatcg
    20701 ataaataagc aaggtgatct acaaaacgag aagacgggtt tgtcaaggaa gaagaaaatt
    20761 gtgcccgttg tcctttttca tatagatcag catttagcag ttcaaattgc tgttgactag
    20821 cttcttcttg ctgaaaagct ttcacgattt tctggttacc gattcgttcg gtcacaaagc
    20881 ccgagatatc cccaataatc ctttgctgtc tatcgaaatt tgtttgtgaa gcacgagcaa
    20941 cgatccaact cacaaggaaa ataattggtg tagaacaaag aacgaccaat gtcagaagtg
    21001 gacttagacg catcataaaa ataaacgaaa gaatgatgat tgccactcca gaaaataatt
    21061 gattgaaggc cgcagaaatt gcaatggaaa tattgtccat atcatttgta aatcgactga
    21121 cgatatttcc gtgggagttt tgatcataat aacgaagtgg caatcgattc agatgagcaa
    21181 atgcatcttt tcttaatttt gcaacggata agtaagctaa ccgattgctt aacacttgga
    21241 tgagccattg actgactacg ttgattaaaa cgataccacc gaatagaata agtaaatgga
    21301 acagtcgatt gaagcggacc tgtccttctc caatcatttg atcgatactt tgaccaattt
    21361 gtaacgtcat cacaactgtt gcaatcccat tgatgattcc tagtatcaca gctagccaaa
    21421 tgtcttttgc ataggcagta aggtaaggaa tgaatcggcg gagcgtctgc aaggataatt
    21481 tttttacttt cataactatt cttcctcctg tgatgcgacg atttcctgat attctgctga
    21541 ttgagcaagt aattcttcat gggtaccctg agcagcaatc ttaccttgat ccatcactaa
    21601 gatatgatgt gcttcttgaa tagagcggac acgttgggag ataataatca ctgtactgcc
    21661 aagataagtc tgtagcgcct tacgcaaatc caagtctgtt tgataatcca acgcacttaa
    21721 agagtcatct aaaatcagta atgctggctt ggcaatcaaa gctcttgcaa tcgtcagtcg
    21781 ctgtttttgt ccgccagaga aatttttccc attttccatg acttttgtat ctaaaccatt
    21841 tggaagctga cggacaaatt cttctgcctg agcgattttc agagcttccc aacattcttt
    21901 ttctgtggcg tctttcttac cccattgaag gttttcacga atagtcccgg taaatagtac
    21961 agaagtttgg ggaacaagag caatttgctg acggagatga tccagctgcc aagaacgaac
    22021 atccattccg tccattataa ctgtaccgga actgacatca tagaaacgcg gaatcaattg
    22081 tgtcaaagtc gttttcccgc ttccggttgg accaatgatt ccaaggactg tatttcgctt
    22141 cagctgaaat gtaatatttt ggagagaaag accagaatca ggagtataac ggaagtcaac
    22201 atggtcaaaa gcgataattg cttcagaaga aaaatcggga gtagtactga cctcagcttc
    22261 aagaatcgaa ttttctgtgt ctagcacctc tttcacccga tttcctgaag cttcggcacg
    22321 tgtgaagatc acgacgaggt ttgaaacaac gatcagtgcc aaaagcattt gattcatata
    22381 attgatcaaa gcaagaactt gtccttgttg caagctacca atatttactt tgatcccgcc
    22441 aactgtcagc aaacagatga tcccgacatt catgatcaag gttgtagcag gtgataatag
    22501 ggcagagata ttggaaacac gtaaataatt gttcgctaga tcatttgtgc ttttatcgaa
    22561 cttctctgtt tctttttttg tctgcgcaaa tgcgcggatg acacgaacac cgctaagatt
    22621 ctgactaacg tgttcattga gctgatctag tttttcttgc actttttggt agagaggaac
    22681 ggtttttttg atgataaaaa ataaaatgat tgagaaaata ggcaataatg ctaagaaaaa
    22741 cagacctact tcccagtcga tcacaaaagc catgaccact gacccgatac ttaaaaacgg
    22801 tgctctgatc aataaacgaa tgaccattgc taaagctagc tgaagctgat tgatgtcgtt
    22861 tgtcattcgg gtgatcaagg tatctgttcc aaaatgattc aactcgttgt gagagaaagt
    22921 attgattttt ttgattagct gattacgtaa ctctgtacca aatccttgtg aagctactga
    22981 agcatagtat tggcaaatga tagcacaaat cagaccaatc acagacatag cgaccatcca
    23041 taagcccatt tcaatgattt ttcctcggtc attcattttt agtccattat cgatcaagga
    23101 agccatcatt aaaggcagga caagttcgaa gacagcttca agaaatttga agaacgggcc
    23161 caaaatgatt tgcttacgat agtcttttgc ataacgtaat aactgaatca tatgattcct
    23221 cctattattt atttgtccac aattgaaaaa acaagtacta ccacattgta gcttaggaaa
    23281 tttagaatga aaagagaaca agccttttca taaatgaaag ttttattatg aagaagcttt
    23341 agaaattgaa tagactattt tttttaaaaa aaa
//
LOCUS       DAYYME010000033        23098 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000033 DAYYME010000000
VERSION     DAYYME010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.033"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..216)
                     /locus_tag="ACHBFW_001069"
     CDS             complement(<1..216)
                     /locus_tag="ACHBFW_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317393.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKIT"
     gene            complement(223..771)
                     /locus_tag="ACHBFW_001070"
     CDS             complement(223..771)
                     /locus_tag="ACHBFW_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFM9317394.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(816..1148)
                     /locus_tag="ACHBFW_001071"
     CDS             complement(816..1148)
                     /locus_tag="ACHBFW_001071"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317395.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(1162..1419)
                     /locus_tag="ACHBFW_001072"
     CDS             complement(1162..1419)
                     /locus_tag="ACHBFW_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317396.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(1614..2156)
                     /locus_tag="ACHBFW_001073"
     CDS             complement(1614..2156)
                     /locus_tag="ACHBFW_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFM9317397.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VGQEAQKIDAIKIYYVRSAY"
     gene            complement(2177..3268)
                     /locus_tag="ACHBFW_001074"
     CDS             complement(2177..3268)
                     /locus_tag="ACHBFW_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM9317398.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNFTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(3276..5486)
                     /locus_tag="ACHBFW_001075"
     CDS             complement(3276..5486)
                     /locus_tag="ACHBFW_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM9317399.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(5502..8030)
                     /locus_tag="ACHBFW_001076"
     CDS             complement(5502..8030)
                     /locus_tag="ACHBFW_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9317400.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(8082..8492)
                     /locus_tag="ACHBFW_001077"
     CDS             complement(8082..8492)
                     /locus_tag="ACHBFW_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFM9317401.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(8505..8723)
                     /locus_tag="ACHBFW_001078"
     CDS             complement(8505..8723)
                     /locus_tag="ACHBFW_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFM9317402.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(8720..9712)
                     /locus_tag="ACHBFW_001079"
     CDS             complement(8720..9712)
                     /locus_tag="ACHBFW_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9317403.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(9791..10702)
                     /locus_tag="ACHBFW_001080"
     CDS             complement(9791..10702)
                     /locus_tag="ACHBFW_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM9317404.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVSAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(10721..11065)
                     /locus_tag="ACHBFW_001081"
     CDS             complement(10721..11065)
                     /locus_tag="ACHBFW_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317405.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(11085..11363)
                     /locus_tag="ACHBFW_001082"
     CDS             complement(11085..11363)
                     /locus_tag="ACHBFW_001082"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317406.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVLQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(11722..12222)
                     /locus_tag="ACHBFW_001083"
     CDS             complement(11722..12222)
                     /locus_tag="ACHBFW_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317407.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(12223..12768)
                     /locus_tag="ACHBFW_001084"
     CDS             complement(12223..12768)
                     /locus_tag="ACHBFW_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317408.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(12765..14021)
                     /locus_tag="ACHBFW_001085"
     CDS             complement(12765..14021)
                     /locus_tag="ACHBFW_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM9317409.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(14381..14692)
                     /locus_tag="ACHBFW_001086"
     CDS             complement(14381..14692)
                     /locus_tag="ACHBFW_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317410.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(14715..16190)
                     /locus_tag="ACHBFW_001087"
     CDS             complement(14715..16190)
                     /locus_tag="ACHBFW_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFM9317411.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(16639..17091)
                     /locus_tag="ACHBFW_001088"
     CDS             complement(16639..17091)
                     /locus_tag="ACHBFW_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317412.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(17093..17458)
                     /locus_tag="ACHBFW_001089"
     CDS             complement(17093..17458)
                     /locus_tag="ACHBFW_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317413.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(17557..21840)
                     /locus_tag="ACHBFW_001090"
     CDS             complement(17557..21840)
                     /locus_tag="ACHBFW_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFM9317414.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(21987..22226)
                     /locus_tag="ACHBFW_001091"
     CDS             complement(21987..22226)
                     /locus_tag="ACHBFW_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317415.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(22316..22558)
                     /locus_tag="ACHBFW_001092"
                     /pseudo
     CDS             complement(22316..22558)
                     /locus_tag="ACHBFW_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22671..22907)
                     /locus_tag="ACHBFW_001093"
     CDS             complement(22671..22907)
                     /locus_tag="ACHBFW_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317416.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
BASE COUNT         6792 a         4999 c         3396 g         7911 t
ORIGIN      
        1 tgttattttt tcattgacta taactagttc atctggtaaa aaaattaacc aacaaacatc
       61 gacaaacagt aaatgatata atccatttcc acctccaagc atattcaata ctggtgaaca
      121 tgccttagaa acacgaatat agttatggtt taaatagata ttttctttaa acattgcttg
      181 tttaaaagcc atatgctctt gttctaaact tttcataaac ttttactcta atgcacagct
      241 agcagcagct gctgcaattc ctgctacaat atttacctta gctaactctt tagctgcagc
      301 ttttcctaat tgtttttctg caatttttaa taataaatcc gcagcttttt caaaattaaa
      361 agcttttaat gcttcaataa aatctccctg cgtacctata gcccaagcca ctcctcctcc
      421 aggaagagaa ttaatggcta cacatccagc atatcgtttc caatcaactt tctccattct
      481 aggaacaata atatgttcat aaaagactct tgctgctctt ccttgttcgt tatcttgact
      541 gattcgttca cgaatagcac caaaatcctt tatatggtat acaccatgtt cgtctaaaaa
      601 accaactgta tcaaaataaa aagttaattc atctactaat ttattcttta tttctaaatc
      661 aactgtattc actttttctg tggcatacac acttgttcca gtactaacaa aagtacttaa
      721 acctaataag aaaacaccta aaacaccaaa tattttaatt attcttttca taatttctcc
      781 tccataaaaa ataagttact ttttaaatat cttgtttatg aattcattcc ccaaaggcct
      841 tttaagattg atacagcagt ccgttcactt cttttagact tgtcatacaa gtcttttaaa
      901 tgatcccata tcagactagt ttgtctatct agcttcaatc cattaaacgc taatgaacgg
      961 ttcatttttt ctattacttt agataaagct acagaaattt ttataccttc acaacgcgca
     1021 tcttggattt caataatagt atcatcaatt agtttttgaa tttcttgcct atcaacatat
     1081 tcaatcttag aaagagaatc ttttaaagct actaatgatt caacgatttc ttttttctct
     1141 tcagacattt tcttcctcct tctagtgacc cgtaaagcca tgtagagcag agtccattga
     1201 accagtaatt acactatcta caacgccatt ccaatttact ctacattttc catttttcag
     1261 atttgtcggt ccatacggat agcctttttg acattcaatg ttcggattat accagatagg
     1321 attataccat tcagccgcag aagcagattc agtcgataca aacaatccca caccaacaaa
     1381 tccaatacac atcacactat agccaattag ttttttcatc tcgtatccat ctcctaaaaa
     1441 atagaatagt tcttatagtt attcataaga catatttttt ccgtttttgt tttatccttt
     1501 ctgtcctata acaacttttt tcaagtaaag aatgacatag tttttcttaa aaaaacagtc
     1561 tgtttttttc tatttcttcg gtgtataata aatatgagtg ttcatttttt attttaatac
     1621 gcactccgta cataataaat cttgatcgca tcaatctttt gagcttcttg acctactttc
     1681 aattgaagta atacaaattg agtaacctta ttcattgttt cttctccaaa taacaaatat
     1741 tgatccggat tctctatatc ttgtgtcacc tggtaaagtt tcccagttgt tttcatttgt
     1801 acatgcgggt ctacatcaat actattgtct ttaatacaac gatctgtcat atagtccctc
     1861 acagcttgat tgcgtttata aacatctgtg aaattactcc attttctagc aaaatctttt
     1921 aagaaatttt ttgtcatttc atctatttgc atttgttgcg ctgtctcgtc ttctaatgaa
     1981 ccaatttgac tgttactttc agtactttga agagttccac ttgtttcttt tactgcttcg
     2041 attgtttctt ctgtcgttct caattgtgtt ttttgttttt cagaagaaaa ccaatgacaa
     2101 ccagtaagac ctatcaaaca taaccaacta aatactaaaa ttatcccttt tttcacgttt
     2161 ctagtcactg cctttcttat ttcaccttac gaaccagact acgaattttt gtttgattgt
     2221 acgtgcgttg ataccgagcc ataatccgtc cttgctcggc attctgttcc acactagaga
     2281 aatgttgtcc gccatatgaa acatctgtta taattccggt gtgaccatag attccaggag
     2341 acaatgcacc acctgcttgc caattcacca catctcctgc ttgtaaatca cttggttttg
     2401 ggttcatgat tacttgccaa ccataggttg tccagttata atcttcccct attaacatcg
     2461 caaacatgtg tccagaaccc attaactgtg ggccacctaa ttgagataca taccatgccg
     2521 taccaccata acattcccca ttatttacgg atcgtccaat gatccgatcc aaaaccttcg
     2581 cattgccttt actttttcct gcaataagcg aagttttttt tgttggtagg ctattccaat
     2641 gcttgatcgc aaaagtatca tattccgtga agttatagga cgttatgata ctcattgctc
     2701 ctcgataata cggactccct tcggtaatac ctgctgccgc atagcctcca cgacctagaa
     2761 tcaataaaca acttccagga tctttattgc ccacacattg attaaagttg ctttggctcg
     2821 ccaccataaa gccataatag tcataacaag cttttaagtt cggaaacttc atatagaaac
     2881 caccttcaga accaccaatt ccacgtgcag aaccttgtgg gtagggacaa ccaggaaacc
     2941 aggtaatccc aaaaaagttc gtatcgtttc ttccagattg tgactgtcca taagctgatt
     3001 catagcctaa ttgagaaaga atcacactcg gtaataactg atactttaca gctgcaccaa
     3061 ctgctgcttg cgcatgttca tctgtgattg gaccacgtcc tttaatctct gaaaaaatcg
     3121 gtaatccttc ggtatattcc ccattaaaag cgacagctag acctgtagct tctcctccat
     3181 gctcagaaag ttctcctgaa aaaaagaatg gcaatagaaa aaagggaaga ataaagaaaa
     3241 gaagacaacc tagacaccct ttcttcattt cttcctcatc ctttcttcga taatggtttt
     3301 tggactgttc gtccttttgg taaagtaaaa tcagttcctg gttttgcgtc caaaatcggc
     3361 agtgttttag gatccttttc tattttttcc gattgctctg ttggcttatc taaagaagaa
     3421 aatgcaggtt gtaacgattt tggtgcggaa atcgacggtt tactttcaga cgaacgttgc
     3481 cccggttgtt gcacagaacg atttcctgat agagtggcta ccgacgaatt aggaacagag
     3541 tattggttca ataactgctg cattgctgga taactcccat ttttcgcctg atttttcact
     3601 tggcgaatct gttgtgaaag agaaccagca gctgttaatt gtggcacggg tacttttcca
     3661 gaaggcaacg gaatcgattg accaacaaaa gcgatacctt tatttaacaa tggttttctt
     3721 tgattattcc cttgttgctt actcaattgt gccaatgatt gtacctgttg ttttggtctt
     3781 actgaattca tggacgaatt tggaatcaaa ccatttgaaa gaccagaatt ccactctgtt
     3841 ttcgatttgg tagaagacat ttcaggctct accccatgat aacgaacatg acgaccacta
     3901 taaggcaagg cttgatttga acgcccacct agttcaaagg gattaaccac ttgcctccct
     3961 tttaccgatt cttgtggtgt acgtttttgt tgaaactttt gaaatccatg tatcgctgca
     4021 gaagcgagtg gattcgttgc tcgatacaca ttccctgcta atcctgcacc tttgattgcc
     4081 aaattttgtc cagcagttga tagttgacca ggcagttgtt tcatttgaga caaagaatga
     4141 cttgctatgt ttttcgtgtt gtttatagtt tgatctgcag ctcctaaccc tagtttcttt
     4201 aataaatctt tccgttttac aatgagtaaa attgatagga gtgctttgac aaacatattt
     4261 cctaaaaaac caaaaacagt attcattgga attaaatcgt ctactatgtt atagatcaaa
     4321 aatgccacca aaataatcac actgtaaaaa gatttttgaa acatcaagcc caaaaaggac
     4381 tttaaggtat tcatgagcat ataatcaaat actggaacaa aagaagcgag taatgaaaaa
     4441 ggaatcacaa gtaataaaca caacaccaac atttgaaaga taaaacgaat catgccaatc
     4501 actacaagtg gtaatccaat acttagatta agcaatggca ccatagaacc aacgactgct
     4561 ttataaacag ctttagttgg ttgaatatag acacgataag ccttcttttt ccctctatct
     4621 tctcctgcag cagtggtcac ttttgtctca atatcagata attcttcact tgacgcttcc
     4681 ttggctagaa attcttgtgg ttcaatctct gcttgataca attcttctaa atttgttgtc
     4741 ccataattta acaatatata cggatcaaca acagccgctt tataataaag ttggcgcact
     4801 ttttgaatgg cttccttttg tgtcgttggc actaaaacac cattgacatc ttttgtcgcc
     4861 aattcatcta gactatttgt agcagaaaaa ataaagcctt ctacctccac ggacaaatca
     4921 tttaaccctt tggtaaaagc ttctccttta ctaggtacgt tactaccata gccaaaccaa
     4981 accattgaaa aaatgacaac gaagagaaat cgaataaaca ccatttttgc gtaatgtttc
     5041 cctctaaata tacgaagagt aaatagatat aacacataca agacgacaat ccccgcacca
     5101 ataggatcgt atatttttga ataaatagta tccgaaaaag tgaaaaactg gtgaattaac
     5161 tcgttcattg cagctccttc atagagcttc tcaatgacaa aatcaaggcc ttgccaaatc
     5221 aatttactta aactaaagaa catattcgtc atcattttta gaaaatctgt gccatttgcc
     5281 ccaaataaca ttttgcctaa tgaaaattca ctatcagata aataagaaga aaaggtatca
     5341 atatgaaatt tcccaatctc atagatcgat tgatccacct gcttttctgc cgcatacacc
     5401 aatccaccaa caaaaagcag tccgaatcca atcgttaaaa aaaacaaaac gcaccatttc
     5461 ttttccttat gctttatata agccattgtt tcacctcatt tctattgcat gattaacgta
     5521 tcctgttctt ctacttgaga gtgacttgtt ttttccgttg tttgtaatgc agcggtcatt
     5581 tcttcaaaca aactgtgtac attgagtttt cctacacgtc catagggatc taacatccaa
     5641 cactgtcctt taatcatgtg gctcatttca gctaagttcg cttcatttac ttccaaacgt
     5701 aaatgattca aaatcttttc tcgctcactg tcttcatcaa aagcaaagac gacaccaaaa
     5761 ttcccttttt ctttatctga acctaagtca ccaataaatt gtgtatctaa cactaattga
     5821 ttcatctggg aacgccccac acgtgccatt gctgcaataa tactacgtcc tgcgttcgaa
     5881 ttattaaata cccatgcttc tgtaaaataa atcgctgttt tcttactact atctcgtttt
     5941 ccaaacattt cacaaaaacg acctaaacta atcatcaaac acaaagactt tcgatccgca
     6001 tctgtatata gttctggtcg gacattgtca tttggtaatt tcaaatcttg gatctctaaa
     6061 atcgttgttt tggcattaaa gtctaatccc gggttttctc cataagaaaa tcctaaacgt
     6121 aaaatcgaat cttctaccgt caagcgtaaa aactcaccat attctcgaac ctctttttgt
     6181 tccatttgtt ctaaatagtc gaccacatgc atcaagccaa cttgttgtcc ggcttctttt
     6241 tgaactaaaa cttcttggat tccctttaat accgcccctt ttaaaaggtg attggtacct
     6301 aacggactta actcatcaat catatcctgg gctacttgtt tcgcatctac tcgggataaa
     6361 caaacaattg gatctaatac accataatta cggggatttg tagcatctaa cgtgacataa
     6421 ctcatttttt gcaaatgttc tacaaacaaa gggtaatgtt tctgataata cgggtcttga
     6481 atgagtttcg taaaccattt gcgcttttct cctttgggat ccacaaataa actttgtaca
     6541 ttcataaacg aactatacaa aaagagcaag ccaactaaaa aggtcttccc tttaccggtt
     6601 tttccagtta ctgctacatg aggcgcatca aacaacgcac ctttgattcc ttccgcaatg
     6661 gctagaagat tcaaaaagac aaaacgattt gacgcataaa ttaattcttc taatggtgtt
     6721 ttttctcctc gagaaccacc atcccctaat gtatcttgcc gaccaatata ccagccggat
     6781 tccataccta attctgatac tgctgaaaac aaccattcgc caactgcttc tacagttgtt
     6841 cggtgcttcc aatgtaactg ccgtcccaat ggttgcccta ataaacattt atagaacaat
     6901 tctcgttgat cattttttgc acggtggata tggataatcg tatgaagttg ttgaataacc
     6961 gttgcactat tttgacgaac ttcttcaacc gtttgaccgt atacaataaa acatgcgatc
     7021 caatcgacga caggttgccc attatctact cgttgcttta aatgttttac taatcctcga
     7081 acagttaata agcgttcact aggttcttca tcattttgaa ccaattcctt atgctctgtt
     7141 ttgaaccgtt ccgatagcca attcagtttt aaaccaatac ctccctgtcc tttcaagcgt
     7201 ggatagtgaa ttttcattcg caattctgct gggaaagaaa gatcctgagc aaattgaaat
     7261 agatcacaat ttctcatatt ttcaggtgtt tccccaatag gcaacaatgc taaataacta
     7321 ctccctaact catcttctaa ttgaagaagg cctttatgtt ccttcggtat acgtacatac
     7381 gttccttgat tgtctttcag tacttcccaa ttattttgtt cttctacttt gtgagccatt
     7441 cctcgaatat attgcagacg attgagatac aacagctctt tttttactaa gggacgaata
     7501 gaaaagctat aaagtaattg tgtcacttct tttacataag gatcactttc agaaaacaaa
     7561 gtttccaaat catctacttc taatccaaat aagcgtgcta aacttcctgc taaatctaac
     7621 cagattagat ttattacatc atgaaaactc tccaccgttg ctttttcttt gactttgatt
     7681 cctaaaataa atgaaggacg atagacttga ccttctcgtt ccaacaatgt cactgtttct
     7741 tttgcataat acgcaccaat tgcttgacta tcgggagaaa atccctttcc taatgtgtca
     7801 aacttcgtcc gtaaattcat tgttcgtgga tacatcacca actccacatc ttcataatct
     7861 gctaaaagca acagtaaacg agccatcatt tcttttattt cctgtgtctt ttttggtgtg
     7921 ttttttgaaa ttgttcgtgg aaaaacatga tagtaggccc agatacaatc ctctccattt
     7981 aacaaacaat ttttcttgaa tgcttttact ggttctttta caagcaccaa tgaatttcac
     8041 ctcccaataa aaaaggaaca accagttgat tgtccctttt tctaatcctc taaataaaca
     8101 tacttattca caaaaagtaa ttcttccaat gcattgtttg ataccttgtc ccagttataa
     8161 tacacacaat caccattcat catacgaatc atataacaaa accaatccca taaataaaaa
     8221 tgcacttttt ttccatcagg ttttagtttt gccagtgcta acacacataa ataggggagt
     8281 cctatataaa aaacaactcc tgctggtgga aaaaaagaaa tgacccaacc aataaatgaa
     8341 cgtaaaaata aatattcaaa gattacgacc aacaaaaaag ttgctgcatt gactaatgca
     8401 actggaaatg gaagacgcca attgcttgta atctgttgta cgcgataagg ttccgcataa
     8461 acaatcgaat aatcataagg ttcccaatcc acttcactat cctcctaatc cataaataat
     8521 ttttcaatga ttttaccaaa cgcatcaaat acacgtgttg gatcatttaa gaagaaataa
     8581 actgcggcac ctgctgcgat agatattaat gcatttccta cttttgattt cataaaattt
     8641 cgcaatccca gataaacaac caccattatt aaaaaatagc caccttgcgt taatataaaa
     8701 tctacaattc ccttcagtgt catttccgtt cttctccttt cctatccaac caattatgtg
     8761 ttaatttttc aacataatac tgatttgttt ttttcgtaat gacaatcgtc atttgttctt
     8821 tttgtaaaag tgacgtatca ccatctcgaa agacgaccgt tgcttttaca ataaaaggtt
     8881 ttttcgtttc tttataacgc aatactttca tctctacttg atcaacttta gctgtttgat
     8941 tgagtccttc aggttctttc atcatgtatg acatatcttt tgcattgttt ttcgcatact
     9001 tatctaagaa atcttccaca aatccttcaa catctttcgc ttccgacgca tcaaccgttt
     9061 cgtataatgt gtcatcataa gtaatcattc gttgtttttc tgtattcttt ggtagtgccg
     9121 taaaataagg ataagcaata atacgacagc ccttgtttga aatagaaaag ggaagattga
     9181 gtacttgtgt gattgtcaca cttgtttctt tcttactctc tggattcatc gagcgaatat
     9241 cataagttac tttataacta gcagttggaa tggtttcaat ttcttttaat ccaataaacg
     9301 aagcagcagt taattttcga acacctgaaa cacttcctgg ttcttgaata aagttttgtg
     9361 cataaaactc tttttttaat gtttcttgac gttgttgttc ttgttcatga ttatcgaatg
     9421 tattcatata caaagcaaca aaacgattca taaaggattc aatttcaggt gtgtacgtta
     9481 attctgaccg actttgtact tctttctttt gtaattgttg gatttcatct ttcaaaaacg
     9541 acacttgaac cttcgttttc atggattgaa aaaatgccaa gccccctgaa aaacaaagaa
     9601 ataaaaccaa acaccaaatc catttcactg ctttttttga atggattttc ttttgcttcg
     9661 gtactttctc tgtataaact ttttctttcc gccgtagttg tcgttttttc ataagctact
     9721 ccttctagta tgtttttact agtacgtacg attgacaacg ataagcttgt ttttctgaaa
     9781 aatagtccat tcaattaccc tcattttcta ttccttttct ctttctttta tatccaacaa
     9841 atccagcaat acttgtcaac actcctccta gaacagaaag tgcaacatgt atttcttcac
     9901 cagtctttgg taagactttt gtttttccat ctgctgtttt gatttcaata ttgccacttg
     9961 gaagtttctt tacaggaatc gacaaatcct ttaccaaatt cccttcacta tcttggatta
    10021 atggaatacc atcttttaca ccaacaattg tttgatcacc tgctactttt aatggggtgg
    10081 ttggtgttgg aatatgggtc aattcctcta cattactagt gacaatcggt acactcattc
    10141 cttgagaagt attcgtcgta atttcaccgc ttggggttac cgatactgtg acttcttttg
    10201 ctgaaacaat aggtttattt tcttttggaa ttgcttctga tttttcaaca ggttgtttcg
    10261 gttcttcttt ttgttcttca ctggtagacg acatcgttgg ctgttccgta cttgacgaat
    10321 cagtattctt ttcagaagaa ctggttgttc ctgattgttc cggagttgtg actatacttg
    10381 aatccgttgt tgttgaacta tgcacacctt cttcttcgat cgaatcagtc ggtaaagttg
    10441 attcaggaaa ctctggttct ggtgtcactg gctctttttc aggtttctct gactgcaatt
    10501 gttcaggttc tgctggtttt tccgttgttc ctccatttag ctctgtctca acatctggta
    10561 atccttcccc tggcaatgtt tctacaattt ctgacttagg tgtttctaca gtttcatctg
    10621 cataaaccaa tgaactccct aattgaaacg aaaataatga aattcctagt gttgttacga
    10681 ctgctaatgt tgtttttttc atgattattc ctccaatttt ttatgcaatt cgtgttttgt
    10741 tcatagacgc cgcactaata ttatctgaac gataaaagaa ctcccgtccg ttttcttcta
    10801 acccaacata tggattattt ttgaataatt gaatcaatag ttcactggtg ttgtattttg
    10861 gatagtttgc aaaaaaataa tactgtgctt gtcgcccatc cgaaaattca acaagtaatt
    10921 cataacgata ccgcttcaat ccttttcgtt gttttaaagg atctctatac caacttgtcc
    10981 atcgttccat tcgtggatag agatagacac gatacacata gaacaatgcc ataccacata
    11041 ccaccaaaaa taataatcgc cacataaaaa ttcccccttt tcttctacct ttcttgaatc
    11101 atcgaacatt cataccctct atagagattt gaaaaatcca tcatcaattg ttcatataaa
    11161 cgttgttgca aatagcgatt gtttcgaata cataattctt catagccatc aatcacttga
    11221 tcttcttttt catagcatct tgtagctcta tgacgctcag cttgcaagac ataaccaaac
    11281 tctgcaccat atcgttctac tgttaataac tgttgactac gcaataatgc aatataacaa
    11341 tcacaaaaat gttcaaccga catttgattt ttctcacgtt tgtgaaatag gtattcttta
    11401 gaaagcacat tattcgttaa acaatctgat aaatagcgat aatctggcgg tcggttattt
    11461 tcatctgtga atttttccca taataaactg atttttattc gttcagcggc tagatagtct
    11521 tgttcgcgtg gataaaattc atcaatcgca caaagttttt tatcacctga ataaacaaat
    11581 ccatataatg cgaaacgaac tcttcctttt caattggctc tcctaaaaat tccagtgaca
    11641 taccatcttc tccttcctaa aaaaaataaa aaagcagaaa ccgatttggc ttctactttt
    11701 ctttcctttt atagctcttt tttacggaag aatccgcaaa tgacattttt tcaaaaaatc
    11761 aatcggcaca agttcacgaa aacttggagc aattttggat agaatcactt ccccatcttg
    11821 taaaatacaa tcataaaaat catcagttac ttgatactta cgcttcttac aataattgat
    11881 aaaatagtag cgatcatttt ctatttccat tcgttgttct actaagcgca tacgaacatc
    11941 atcatagaaa taagccacaa atcgcccttc aagttcatgt ttttcaatgg gatactcttc
    12001 tggtaacaat ctattttttc gtcgtttata cattgctgga taccttagaa gaagttcatc
    12061 taaacgtctg tcagtatatg gtaactgacg aatcaacgat tctaatcgtt ctttatacag
    12121 atagaataat tggcgcaagc aatagtgtac cgcctctgga ttttctaata ctgttggatc
    12181 acgtgaccaa tacgcaccaa cataaatcat aatttttttc atttagacaa tcacaccagc
    12241 aatactaatg accgatccac aataggtaga acaagtggca tagcgaccaa tttcctctaa
    12301 ttctatctca atctcttcta aagaaagata tgtctgttct tctgttggct ggcgactcat
    12361 ttcccaagtg acagtcacca atgaaatttc ttccataaga ggtttttcgc tgctaagtgc
    12421 tgccacacag ttttcatata tcttcatttt atcgcagcct tttttaaccg ctaaaatcgc
    12481 aggtattggc aatgaaaatc gataaaagcg aatgaattct agccttcctt tccttcggct
    12541 aaattccaac tcttcgagtt cttgttcgta tcccttacga acttctaata agcgtgcaat
    12601 ttcttgccag ttctcttttt cctgataacg tccagctttt accgcttgtt caatataagg
    12661 aactcgaaaa cgaaaataag acaaatcaac catcttttca tttaatgctt cgattgcagc
    12721 ctttaaatcc aacaggcgca gccattctgt tgtcgtatat gcattcatgc aattttccct
    12781 cctgacgttc ccatccattc tagatagttt tgttgctcgt catttagttc ggcattatca
    12841 atcccttctt gtacaaagtc tcttcctaaa atacgatctg cttcttgtaa caactttaat
    12901 gtcggagcta cttgtttcaa aaaccagcgt tctgaacgat ctaatgagaa tttttctggc
    12961 tttactgtaa aatccaattc ttccatcgaa cgaaataatt ttgcccatcg tttatcaaca
    13021 atccgtgcat cactttccag tctatcccaa acaataagtt ttgcattaat taaaccaacg
    13081 ccaatctctc ctaaacgttt cggtgaatca cctaaaataa atgcttctac tgcttgtgtt
    13141 gctttctcat tggctaaccg taactcatat cgattataaa taccatattt cgttaatgct
    13201 tctaattctg aaattttttc tcggttcgca atttcaaaac gtttctcata aaaattaaaa
    13261 aagagaggac tctttcgcga tccaaaatac aaagataatc cttcacttcg aaaatagcca
    13321 tcatctaaaa aacaaccgcc aatattctgg aatctcttaa agtccatttc taataaacct
    13381 gctttttgtt tttctagtaa ttcaaagaga tcgaaatgtt cttccgtttc caatccccaa
    13441 agttcatcta acgcaatatc gaatcggcta cattgcacac gtgtataacc agcttccaac
    13501 tttccttgat ttttccaaat ggaatatagc catgttcgcc aatctgtttg ttgttcacgc
    13561 atgacttcct catattcccg gcaaccttgt cctgaaaatt gcacaagtac attttctgtt
    13621 acagttcgtt ttgaatgcca gaacacccag atattcccac gaacagaaag acagtcaaaa
    13681 agatacaagc ccgttggacg tgtttcaaat tctgtcagat ccattcgtaa tacacgttga
    13741 atcactgttt tgtagtctgt cccaaaaaag gaaagacgca aataatcaat cattgctcga
    13801 agatctattc cgcttttcat atacaatcga aagcgttcct tgattcgttg ttcaaaatcc
    13861 tctgggattt ttcttttccc ttgttccatc tttctaatgt aatcttttga gcagcccatc
    13921 aattgcgcaa atcttgcttg tgatagcggc tttttatctc cacttatatt atgaattgca
    13981 cttaacttct ttctgtagct ttttagctca atcggtgtca ttttctcgct ccttttataa
    14041 aataacaaaa tcgatttttt taacccttta gaaacaccga tatatcaact agtccccctc
    14101 gttttttggg aaaagtagta caactgctga ccttagataa tcacttgata caaaagggtt
    14161 tataaaactt ttttctatgt gttatgttat ttctgccccc catattatat atcggggggc
    14221 tttacacaaa gaggccgtgc gcaagcgcac gacctctttg tgagctgcac acgctgctag
    14281 cgagtgcgca ttataggccc atgcttcgca gccttaatgc gcaccgccag gcagagtgcg
    14341 gctaccagac tacgtctaga gccctcccga tagataataa ctacaaaatt aaatgatcta
    14401 attttgttat ttcactatat ttttcaggag aaaaaacgcg ataaattgac aaagttaaat
    14461 cctcttctaa atctactcga taatgataca atcgagtcaa tcgttctata tcttttttcg
    14521 ttagcaatac ttgattaata acttttcctg taaaaacacg ttctttgcgc aaaaagcggt
    14581 aatcagttac ttgttcaata cgattcaacc aatcataaaa cgttctggaa gaaagagaag
    14641 gattaatcat tcgtattgct gtagctactc tataataaca actctctgcc atttaacttc
    14701 acctccttag ttgattacaa aatatgcgca tcagaaaaac ttgttccttt tggatctttt
    14761 gctaaataca tttggcgctc tagttgttct tgtaacaact gattatcatc tcccatcgtt
    14821 tgatcttgac tatcctcttt tactgtaaaa tcatagacaa tcatttcagt aaatttctca
    14881 aattctttga taaagtgata atctttgtaa acaaatggcg caaaaaactc tacaggtaca
    14941 tcaaatcctg gaaaagcata tccacgacca ggttgatctt tgttgtaaaa tggcttattt
    15001 ttaaaatctt ctccaaaagt cattaaataa ccaatgggag acaatcgacc aacagaaata
    15061 cggaatgtta aattatcccg aacaccactt ggaaaatatt ctgcatctgg tcgttgcatt
    15121 gctaaaataa tgtacatccc tgcttgacga ccctttaata ctaatggctg aatcaattta
    15181 tcgactctat ttctcgcttc catatccaac gaaccataaa aagctgccca ttcatcaaaa
    15241 aagataaaat gcggtggtaa cccataataa gcataatttt tccctggttc atatttagga
    15301 tctttcatgg ttttcatata ggcataccgt tgattcattt cttttaatgc atttcgtaaa
    15361 caaataatca tttcttcatt tattttgtaa aacacatgtc ctttgaatac atcgacgtca
    15421 cctaatgctg ctaaatctgc cgcttttcca tcgcaaatat ctactgtccc aacacttaaa
    15481 caagcttgaa ttaaagaata caataaatag gttttcccac caccgattgc accagcaatc
    15541 aacatatttg gtgattgatc atatacccaa tccaaagttt tcattaaatg aatacgtcca
    15601 ttttctgcta tacattgatc aataggaatc cgaccagaat ctgaaaggaa tacataattg
    15661 attcgggctt tttctcgttc ttcacgaatt aaatcacgca aaaatacttc agataacaaa
    15721 tgacccattt ttaaaaatcg ttcgtgaaaa cgcccaccat ctaaaggaaa agaaaccact
    15781 aattcttgtt tcttttgata aaaataaact tttggtaatc tcattttttc ccttgtttgt
    15841 ttctcactaa cttgtttctt tgtataaaca aaccgattgt caattaacat tcgaacaagt
    15901 aactgacgcc aataaacaga ggcaaaatat ccttctttta gtcgcgacca atgcatcaac
    15961 cctaaaatta acccactaac cagcaacaaa tacaacacaa tacttattcc aggaataaga
    16021 tacatttgcc aattcgaaaa cgtacttaaa cgtggataaa atactttcca actaaaccaa
    16081 cctaacagaa tcaaaaatgg aaacaaaaag aaaccataat agcaaaacca taaataacga
    16141 ttgaaatagc gaattcgccg accacgatac ttgaaaaaat gagctttcaa ttgtcacatt
    16201 cctcctttct gtttttactt ttcctgtgct aaaaactttt gacaaaaagc aactaataca
    16261 ggaatatcac atttagtaaa aaaacataat cgaaaaagat ttaatcgatt tttataaggg
    16321 tgtgcacaca tattacatat tcgtactata cgaatccaaa tcacttcctg atgatcttgt
    16381 ttccactggt acggtaatga aaaacgaaca agatcacgta aaaacataca ctgcccaata
    16441 accgcacaaa tatatgcatt tgtttgtaca aaaatcgttg gattgtctcc aaaatactca
    16501 atcaattgtt ggatattttg ataacaagca caatctctca aagtatttga ttggttttat
    16561 ctgaattcaa aatctcccct tccttctatc ttagataaaa agaataggaa gactccttcc
    16621 tattctttca aaaaaacctt attttttctc tttttcctta gaatcaacac ttggttgttt
    16681 ttgatctaaa cctttttctt gtgattgttt catattagga ttatttgttg gagcttctac
    16741 tttctctaat cctttcgcat aaatcactaa atctgaatat tcacgattgc catcttgtgc
    16801 tggaatatat accacatgat attctggatc aattaatttc acaggatcac gtcgtttaaa
    16861 accagtcaaa tctacatctg ctggaaattt aactcgtcgt gcaatctttg gttcatctgg
    16921 atttccaaaa actaattgtg ctcgctgtga tcgaatttct cccgtataat cgcctgtttc
    16981 attatcaaat acacgatcag actcttctaa tgcgccaaag cgtacggctc ctaaacaagt
    17041 ttcatctaat gcttctgtaa tgtcataatt atttggaaat tttaatccca tcttacaaaa
    17101 cctccccaat ttttacacca gttaaaaaga caccaacatc ttgatagcca gttgttcgac
    17161 gctctgtttg tcccatatca tttgtttcac gactattcac tcgttcttta cgaaaaacaa
    17221 aataagcttc ttcaaatgaa atacgcacct ctttttctgg taatcgtaat cttttatcct
    17281 cttcagctgt gagataaaca aaaaaagcag gctgttcttc tcgcgaacac tctgcttgta
    17341 attgaacata tcgcccagac acaccattaa tttcctttgg tggattaggt tcccgtgaac
    17401 ccaataaaaa tacttgacct aaaaactctt tcttcatacg caatgctttt tctgccataa
    17461 agtttttcct cctcttaaaa accaaaataa attctaaata tttctacatt acaatcaaac
    17521 aagcggttaa aaatcctaac cgcttgttcc tcatgactaa tctttggctt tacgtacttt
    17581 ataaagtgtt atacctaaag caatgactaa tactactaat ccaagaacca taagtatact
    17641 acttgtttgt tcacctgttt ttggcaacat tggtacaaca gtagctggtt taacccgttg
    17701 ttctggtttc actggttgcg gtggttttgg cgcttccaca atttgaaccg tttgatcttt
    17761 gtttgttaga tctacattgt gtttcgccac ttcttcaaac gtttctttct catcttccgt
    17821 tttcgcttgg agccattccg caaaactcaa gccttttcct gcaagattgc gaccgtccac
    17881 taagaattca aataagaatt ccatcatttc tttatcggcc gcaaagactt tttccgcatg
    17941 agtgccttca aatggtgtgc cgtctggtgt taagtagcct tgaacaatta agcgatagat
    18001 ttgaccaatc gcaagaccgt ctactttgac ccattcaagc acagtttgat ttggcaaggc
    18061 caagatctct tttccgccat ttttattcgt cgctttcgtc gtaatggttg gattactgat
    18121 tttgatggtt tggtcattgt cggtaatatc tgcgtgagaa gccaataata cgccttctct
    18181 aaacagatct tcaaagacca cgacttcttt gcctcgcaac gcacgtgcgt ctagaataaa
    18241 gtctagattg actgtaccat ccgttgtttc tgccacaaac gttaaggtac ttgtgacttc
    18301 ttttccatct actagcactg gtttttccgt tactttgtcc attagaacac cgttgactgt
    18361 gtatgttttt ccaacgacca aatcgtggta tttcacagcg tctgtaatgg tgactttcgt
    18421 taatggatta acctctttca agccagtggg tttatgtgta gccgttgtac gtacacttgg
    18481 gttagttact cgtaccgttt gccctttgtc gttgatgtct ttatggtttg ctagttcttc
    18541 atcatttcta aaaagacttt caaacacgac gatttcacga ccatttatac ctgtcaaatc
    18601 aaaggtaaat ggtacgatta ccgtttcatt ttctgctttg gcaataaagc caacactcgt
    18661 ttctaccgta tttccatgca caagcaaggg ttcaccggtt gctttatcca ttaagatccc
    18721 tttgaccaag tatgcttttc ctggaagaat ctcggtaatt tcaacggtat cgtttaaggt
    18781 aaacaatcct gttgggtctg cgactgtcaa tccgtcttca aagacagccg tggtatgaac
    18841 ggttggatta ttcactttca cactttgggc ttcatcgttg atatccgcat gtgctgcaac
    18901 ttcaatgcca tcacggaaaa gtttttcaaa aacaacgatc gtttgacctt tcagaacact
    18961 agcattaacc acaaattcaa ggtcttttgt gccattgact ttgtctgcaa taaaggccaa
    19021 ggtacttgtg atttctttgc catcttgtaa aattggcgca ttcgttgcct tgttcatcaa
    19081 tactcctgaa atggtgtatt cttttccgac aaacaagttg gtataagaaa cactgtcttt
    19141 aatgacgatc gtttctaatg gatcaaccac tttttcacca tcggctttat ttgacgcttg
    19201 tgtttctagt tttggtggaa tcaagtaatt acgtacagaa accactaacg tttccccatc
    19261 atctggcgca ttttgtttga atgcaaccgt gaatgcttgt ttatctgtat ttggttggaa
    19321 actttctaat ggggcatttt ccaagaagta atagtcatcg gcaactagac ctttcactaa
    19381 aatttccccg ttttgatcag tggtatattc accaattgct tgatttttac cgttatacaa
    19441 ggtaaagttc acacctgcga gcggcgtttt cttcttgttc cattcttcat acttgaataa
    19501 ttttacactt ttccgtgctt gacggttcgc aaaatggatc actttgatcg gttctgtatc
    19561 attggtaatc gagaacacta atgggttttg attcaaaacg taatttacgg gggctttgat
    19621 ttcttccaaa cgatagcgat cttttccata agtaagtgta ccgttagtta ggaaaaagcc
    19681 tttttcattg gttttaaaga tatccgttgt ttctgtatca tttggaatct ccattgtgac
    19741 ataacgattg gctaaagtat caaaaatctt gaattctgta ttagcaagtg gaatcacttt
    19801 tcctgtttct tgatccactt tcaccacttt aagacgttgt tcaattactt tatcttctaa
    19861 aacataatgg aaagtttgcc cattttcaga aattgtgact tcaagtggtt caaatagttt
    19921 atacccttct tttcctttgg tttcagaaag aatgtacgta tcaaagacaa cggtattttt
    19981 accaaaagta gcataaccat cattgtctgt cacaacggtt ttcaccactt tgccagttgt
    20041 ttttgacgtc aaagtgattt caatgccagc taattttggt ttgttatctg gattcgttga
    20101 atcgactaaa tctttattag caactttcac taaatcaatg gttcctttga tcacttgatc
    20161 ttttgcggtc gcattgacag tttttccagg ttcaacaaca atttcaattg cttcatcaga
    20221 aagcacataa ccttttggcg ctttgatttc ttttaaaaca tatttgccag gatcaagatc
    20281 tttaatttct gcctgtactt tgccgttaat ttttttcaag gtaatttctt taatcttttt
    20341 cccatctgct tgatacaaac catagaccgc attttctaaa gaagctgcac cttgtgcttt
    20401 cacaccagtt tccgcatctt ctttaaacac acgaatgttt ccttgtttta gaatttgtaa
    20461 attaataaca gacgcattag gttctgttgg atgtaacaca ccaattgttt gagctcctgg
    20521 acgacgatag gctaatggcg caccttgaac tttaatttta ttcaattgaa tttttgcttt
    20581 atccggtgcc tcttttgtga ctgtaatcca tacttcattg ccgtgaatag atacattgac
    20641 accttttggt ttttgaatgt ctttcacata tttcaaaaaa tcatttttat tctttacttt
    20701 caacgtttcg ccaattttta ccgtatgtgt ttgattatta aaagttgctt tttcaccaaa
    20761 gttattaatc aaacgttgaa tttctgcttt tcgacgatca taatcaacac ggatattttt
    20821 gattgggatt tttgtgccca aagcttccca aatcataaat tgtgccgcaa tgtattgttc
    20881 atcggatttg tctttatttg tgacatatcc taaataagag atcaagcttg tttttttccg
    20941 tacatttgaa tttttcaaat agtttttcaa agcagtagct gtataaactt gaccttctgc
    21001 ggctgagaca ccaggctcaa tacaaaacgc cacttgtcca ttaatctcaa aacgaaccat
    21061 ataatttaaa gtgaagttta gattgttcgg actagaagta acaccaatat tttcgcccca
    21121 atcaattttc cgaccaccta aaacagctgc attggttgca cggcgaacac gaaccgaacg
    21181 attggttggt actgttacta acagtgtagg tgtacctgtt tttggaatgt tctgctcttt
    21241 tttcttttga ttttcaatac tagccgtaga ttctttgttt ggtttcgttg gttgattttg
    21301 actagctgcg gttgaagaga ttgttttttc aacgttttcc tcattttttc cttctttgtc
    21361 agcagtagcc gctggttgtt ccactgtttc ttctattcgt ttcttttctt ccgcttgttt
    21421 ctcagaagac tctggctgca cagaaggcgt tgcttcggca gctttttctc cttctagagt
    21481 ggacgtttct gaagcagggg ttgtaattga ggcagtttct tcaactggtt gtgaagtacc
    21541 aggactattc cctgggtgtt gtgtttcccc aatcgtctga tcgagagtcg ttttggaatt
    21601 gaccgttggc gcttctgtca caggggcctg ttcaacagtg gcttcaccag ctgggacccc
    21661 ggcttctgct ggttggactt ctgtcacttc agaagcgact tgggcaatgg cttccatcgt
    21721 aggttgtata gccgtcgttt cttctgcatg tgcttgagca gttcccacca agaaacagaa
    21781 ttgagaaaaa gccaccaatg cgagtgcttt caaccaattc attttcgttt ttgcatacac
    21841 aagaattcct ccttttcctg agaattttat ataataaaac aaaacgccgt taccaaaagt
    21901 gataacgacg tgaactgtct cttctatttt tatctggcgc tcttatctga acacatttca
    21961 tcaatgattt taccctccca ctataattac agaacaaaaa aagaacctgc ttcgtggaaa
    22021 tcaactgtcc atttcccaat tggaatacca gcttttttgg caacttcacc atcaccaaat
    22081 ctcatgccat accaattact accttcatcc ttaatttctt tccaaccata tgacatagct
    22141 tcatctaatg aatcaaaaat catgacacta ttgccaattg ttacaggatc tggcttaaga
    22201 tcttctggaa gagaaatatc aatcatacca taatgatccg attcctcagt attattgtca
    22261 ttactagcta ctggtttggt tgttctactg gcttattttg atacaacaag tactcttatt
    22321 cttcttttac ttctccgttt tcaatttccc attcaaacca ctccgcaaag tcttgcttaa
    22381 aaccatctac tcgataccta ttttgtaaat aggcatcttc tgacgtcgta ggatctaaat
    22441 aggtaggttt attctctgga cgagcataga cacctaccca tgtttcgtaa tctccttcaa
    22501 aataactatc taccaaatag cctttttttc taagaatctc ccgaacttga tttttaatta
    22561 ttgatacttg ttcaaaacca tcactcattc tatcacctca aataactcta gattctcata
    22621 aatattcccg acaatttcaa tcctatcaat gatctctaac aaatcgctac ttatgcttcc
    22681 ccattcgtat aagaatttcc cttccgcaaa ataaattttc cataaacttc ttcatgatcg
    22741 tcccaaccaa tatcactctc gaaaaattcc acgttgttct tatcttttag ctcagtaaat
    22801 gccataagtt caacatgttt tccaacctcg aaacaatcac accacccatc aaccgttaca
    22861 cgtgtgcctt caatacctaa gaaactttgt cctcgtgtat taaacactaa aactttccgc
    22921 attttttgct cattaattaa tcacgcgcga aatctaggaa tcacaactta ttcgcttctt
    22981 ctttataaat aaaaaaactc caataatcgg aaattactat cttttcctca ttcctttttt
    23041 aaatcttcat acttcttctt atctttgtcc taacctaata acacaaattt tgaagaaa
//
LOCUS       DAYYME010000034        22977 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000034 DAYYME010000000
VERSION     DAYYME010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.034"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            11..493
                     /locus_tag="ACHBFW_001094"
     CDS             11..493
                     /locus_tag="ACHBFW_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317417.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            690..1580
                     /locus_tag="ACHBFW_001095"
     CDS             690..1580
                     /locus_tag="ACHBFW_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFM9317418.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            1906..4380
                     /locus_tag="ACHBFW_001096"
     CDS             1906..4380
                     /locus_tag="ACHBFW_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9317419.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(4483..6219)
                     /locus_tag="ACHBFW_001097"
     CDS             complement(4483..6219)
                     /locus_tag="ACHBFW_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9317420.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6216..6920)
                     /locus_tag="ACHBFW_001098"
     CDS             complement(6216..6920)
                     /locus_tag="ACHBFW_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9317421.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7051..7554
                     /locus_tag="ACHBFW_001099"
     CDS             7051..7554
                     /locus_tag="ACHBFW_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFM9317422.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            7514..7852
                     /locus_tag="ACHBFW_001100"
     CDS             7514..7852
                     /locus_tag="ACHBFW_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFM9317423.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7873..9291
                     /gene="ffh"
                     /locus_tag="ACHBFW_001101"
     CDS             7873..9291
                     /gene="ffh"
                     /locus_tag="ACHBFW_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFM9317424.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9403..9981
                     /locus_tag="ACHBFW_001102"
     CDS             9403..9981
                     /locus_tag="ACHBFW_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFM9317425.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            9985..10506
                     /locus_tag="ACHBFW_001103"
     CDS             9985..10506
                     /locus_tag="ACHBFW_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFM9317426.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(10585..11139)
                     /locus_tag="ACHBFW_001104"
     CDS             complement(10585..11139)
                     /locus_tag="ACHBFW_001104"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM9317427.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11392..11667
                     /gene="rpsP"
                     /locus_tag="ACHBFW_001105"
     CDS             11392..11667
                     /gene="rpsP"
                     /locus_tag="ACHBFW_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFM9317428.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            11679..11930
                     /locus_tag="ACHBFW_001106"
     CDS             11679..11930
                     /locus_tag="ACHBFW_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFM9317429.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12054..12845
                     /locus_tag="ACHBFW_001107"
     CDS             12054..12845
                     /locus_tag="ACHBFW_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317430.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13015..13536
                     /gene="rimM"
                     /locus_tag="ACHBFW_001108"
     CDS             13015..13536
                     /gene="rimM"
                     /locus_tag="ACHBFW_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFM9317431.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            13526..14287
                     /gene="trmD"
                     /locus_tag="ACHBFW_001109"
     CDS             13526..14287
                     /gene="trmD"
                     /locus_tag="ACHBFW_001109"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFM9317432.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            14423..14770
                     /gene="rplS"
                     /locus_tag="ACHBFW_001110"
     CDS             14423..14770
                     /gene="rplS"
                     /locus_tag="ACHBFW_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFM9317433.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            15080..15322
                     /locus_tag="ACHBFW_001111"
     CDS             15080..15322
                     /locus_tag="ACHBFW_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFM9317434.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(15410..15556)
                     /locus_tag="ACHBFW_001112"
                     /pseudo
     CDS             complement(15410..15556)
                     /locus_tag="ACHBFW_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            15613..15927
                     /locus_tag="ACHBFW_001113"
                     /pseudo
     CDS             15613..15927
                     /locus_tag="ACHBFW_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            15956..17215
                     /locus_tag="ACHBFW_001114"
     CDS             15956..17215
                     /locus_tag="ACHBFW_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9317435.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            17268..18164
                     /locus_tag="ACHBFW_001115"
     CDS             17268..18164
                     /locus_tag="ACHBFW_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9317436.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            18175..19044
                     /locus_tag="ACHBFW_001116"
     CDS             18175..19044
                     /locus_tag="ACHBFW_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9317437.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            19054..19729
                     /locus_tag="ACHBFW_001117"
                     /pseudo
     CDS             19054..19729
                     /locus_tag="ACHBFW_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            19935..21599
                     /gene="alsS"
                     /locus_tag="ACHBFW_001118"
     CDS             19935..21599
                     /gene="alsS"
                     /locus_tag="ACHBFW_001118"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFM9317438.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            21612..22322
                     /gene="budA"
                     /locus_tag="ACHBFW_001119"
     CDS             21612..22322
                     /gene="budA"
                     /locus_tag="ACHBFW_001119"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFM9317439.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            22452..22949
                     /locus_tag="ACHBFW_001120"
     CDS             22452..22949
                     /locus_tag="ACHBFW_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFM9317440.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT         7562 a         3980 c         4803 g         6632 t
ORIGIN      
        1 gggaacgtta atgagaagaa aagcagtaat cgatcggctt tatttatttt tttggttttt
       61 tcctatcgtt ttgctacctt ttacatgggt aaatgctgtt gtacaaggtg cggagatatt
      121 aggcctgatc caaggaagcg gattagtggt ttctaggaat tttccagctt tgactgtttt
      181 ctatttagtg ataagtgtag gtctcttatt accaaaatct ctgatcaatc actacaattg
      241 gcagctgctt tacttttctt gcttattagt atttacatct atgttttctt tgctgcctcg
      301 cttgattatc ggccctaatg gtttgagcga gagtggattg aaggattcag cttattcctc
      361 catgtatttc ttgacgatca aaccatggtt ctatttagta attgcctcat ttattcttgc
      421 aacatttttt tacttctgga cagaaatcag gataaaaagt gaacgatctg tatctataaa
      481 aaataactat tgatggtgga tcatcagtaa aaaagcatat gagctgcgta aaatgcatgt
      541 ttctcatatg ctttttttat tattatgtaa agaatgcgaa aaacgtttac atgattttta
      601 cataacttaa ctatattttt acagcaacca tacgagggat ttacagtcga ctgataaact
      661 ttagttgtaa ataagaaagg agcaataaaa tgaaaaaagt attgtttact ctaggtatga
      721 tgggaattct tttagccgga tgcggctcag gatcaggaaa tacagatggc tctgccacac
      781 aaaataattc aagtgactca aatgaacaag taaaaatcgt tgcggtaggt tcgacagcat
      841 tacagccgtt agtcgatgca gcacaagaaa gctttgtaca agaaaatcca aattatcaaa
      901 tctctgtaca aggaggagga agcggaactg gtttgagcca agtcgaagct ggagcagtta
      961 ctatcggaaa ctctgatgta tttgcagaag aaagagacgg agttgatgct tcaaaattag
     1021 tggatcataa agtagcagtg gtcgggatgg caccaatcgt caataaagat acagatgtca
     1081 aagatatcac caaacaggaa ttgattgata tctttacagg aaaaatcact aactggaagg
     1141 aagttggcgg gaaagatcaa aaaatcaacg tcgtgaaccg tgcaaacggc agcggtaccc
     1201 gcgcgacatt cgaaaaatgg ggactggatg gtgctacccc tgtgcagtcc caagaacaag
     1261 attcatcagg aacagttcgg cagctggtaa gtcaaacacc aggagcaatc agctatttag
     1321 ctttctcata tctcgatgat tccacacaag cactaagtat cgacggtgta gaaccaaaag
     1381 aagagaatgt agcagataac agctggggaa tctggtcata tgaacatatg tacacgaacg
     1441 gaaaaccttc ccctgaagtc caaaaattct tagactacat gatgacagaa gaaatccaag
     1501 aaggacctgt caaagaattg ggctatttac caatcacaat gatggaagta gaacgtgatc
     1561 acgaaggaaa tatcaaataa ctagcagata aacatgccac ggtagtcgtt actattaagg
     1621 aaaaatcaac tatgtttccc cacatataca tttttccttt aattgtgaaa aatagttcct
     1681 catattttgc cgttttttga gctaataccg agggtcagct gattgaacac cgtttattcg
     1741 aaaaaaagag ctacgacata gaactgtcgt agcttttttt ttgctatatg acaggaatga
     1801 aaggtgtttg caagcttaat gtgaggaaca ctttccatat ctgttctata ctggatagga
     1861 gaagtttgga aatatccaga aaacgaaatc aatcgaggta gccacatgaa gaataaatgg
     1921 ataaatcgaa gtaaaaaaaa gaaagtaaca caaagatcat cagataaagt catccgtctt
     1981 tttccatcgt ataaagaagg attaacggca gatcaagtca aggaacgaat agaaaaaggt
     2041 gcagcaaata attcagtcga tcccacattc aaaacaaatc agcaaatcgt attggaaaac
     2101 atatttacgt atttcaattt gatctttctt atcttagcca ttttgctttg tttagtagaa
     2161 tcctataaga acttgacttt tttaccagtt atcatagcca atacaggaat cgccattttt
     2221 caggaaattc gatccaaaaa gatcttggac gaactaaatg ttttaaatac agcaaaagtc
     2281 aatgtcatac gagacgggat agaacaaaca atagatatag aagaccttgt aatagatgac
     2341 attgttattt tagagactgg acatcaaatt ccagcagatg cagaggtcgt ggaagggaaa
     2401 ctgcaagtta atgaggcttt attgacaggc gaagcagatg aaataaccaa agaaataggc
     2461 gatccattaa tgtccggcag tttcattgtt tccggaaaag cttacgcccg attagacaaa
     2521 gttggagcag attcgtatat ttcccagctg acagtcaaag ccaagacaat gggagaagga
     2581 gaacagtcgg agatgattgc ttctcttaat caattgatca aatggattgg gatcatcatt
     2641 attcctatcg gtatcctgct tttttctcaa agttactttt ttaatggaaa cacgatcaaa
     2701 gaaagtgtcg ttgcaatgga agcagcattg atcggtatga tacctgaagg actgtacttg
     2761 ctgacgacga ttgcattagc attaagtgcg actaaactag caaagcaaag agttctttta
     2821 cacaatatga aaagcattga gacattggca agggtaaatg tcctttgtgt agataaaaca
     2881 ggaacgatca cagaaaataa aatgtctgtt caaaaagtga tcgtctctaa aaaacaagaa
     2941 ataatcagtc agtcagatca attggaagca tgcattgctg actatgcaaa agcgatggca
     3001 ggagacaatg caacaatgga agctgttaaa gcatatttta caaaaaccac tgatcaatcc
     3061 tatcaccatg ttattccttt ttcttctgtc caaaaattca gcagtgtagc gttggcagac
     3121 aaggtgtatg ttttaggagc gcccgaaatg gttttgcgcg atcaattgcc ggaatatgcg
     3181 gaagagtttg tttctttcgc tgaaaaaggc tatcgcgtgt tggtctttgg tgtgtacgat
     3241 gggattcttg aagaaccagt attgacagaa gcagtcacac cattaggcta tattctgatt
     3301 gctaatccta tcagaaaaga agcaagagcc acatttgatt acttcaaaaa acagcaagta
     3361 gccatcaaag tgatttctgg agataatccg ctcaccgtat ccaatgtagc tgtccaagca
     3421 gggatttcac atgctgatca gtacgttgat gcatctaaat tatcagaaga agaatatgaa
     3481 gcagctgtcg aaaaatatac agtttttgga cgcgtcaagc cagaacaaaa gaaaatcttc
     3541 gttcaattgc taaagcgtcg taacaataca gtagctatga caggtgatgg cgtgaatgat
     3601 attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa tgaagccgcc
     3661 atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc agaagttgtt
     3721 gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt tttagtgaag
     3781 aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac ctatccttta
     3841 gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc tttcttgctt
     3901 gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt gatggaaaaa
     3961 gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat ctgcggggtg
     4021 actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt gattgcagta
     4081 gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag tcagatcatt
     4141 cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct gttttcaatc
     4201 accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc ggcagattcg
     4261 atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa tgaaaaagag
     4321 caaaagccgt ttagtcgcgt agttaaaaaa tttctatcga tatttaaacg agaaaattga
     4381 cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt tcgaagcagg
     4441 atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt tcggaatggt
     4501 aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac catgaaggag
     4561 ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat gccggcttcg
     4621 cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa tgtcgatacc
     4681 tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc ggataataat
     4741 ctcgctgtcg ctttttgaat attggacagc attttcaatc agatttttga taattggata
     4801 aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa tcgtcaaccg
     4861 tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag gttctgtata
     4921 aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga tttcccgaat
     4981 cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag tcagagtttg
     5041 cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg aagtcacagg
     5101 agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt ctaaacgtcg
     5161 cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg aattagtaaa
     5221 gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct cctgatggat
     5281 cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct cttgaatgac
     5341 ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct gcatctttgg
     5401 attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa tcatcagttc
     5461 gttcagtaac gtatataatt gctcttctgt ggtagtatac gcccgataca tcttactcat
     5521 ttgttcgctc aacgcattga tcgtctgata caattcttcc cattgatcgg gtgtttgcat
     5581 gattacttcc gcctgattag gattttgcac catttttttt agtacgggca gcacggtttt
     5641 caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa caaggaagaa
     5701 agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta aaaaaccgct
     5761 tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga ctggtaatgc
     5821 gacatataaa agatccccat tcaaagtagt acttttgcgc aaagcgctac ctaggttccc
     5881 accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca atgtttcatt
     5941 gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt cgttcgactg
     6001 atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt cgacatctaa
     6061 ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt ccacgatctg
     6121 ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa ctaaaagaat
     6181 aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc ctgaaactta
     6241 tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg ctcgattttt
     6301 tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa gtcaaaattc
     6361 caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt catgaaataa
     6421 acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac ggtcacttga
     6481 taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc agcttcatct
     6541 tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc taatacttcg
     6601 cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc aatgatccga
     6661 tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt ttcttgacgc
     6721 agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa aatgatgaaa
     6781 tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc actggttacc
     6841 ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg ttcatcatca
     6901 acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg tttatatttt
     6961 agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata ataggaaggc
     7021 actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg tttaggcggt
     7081 ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga actagttgaa
     7141 actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat tcctagagat
     7201 cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc aaaagtatgg
     7261 acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa agcctatcaa
     7321 aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc ttcatgcgga
     7381 acccattcga tttatgacgg gagtttttcc ggaataaaaa aaaatggtgc cggagtggct
     7441 actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg gctgaaaact
     7501 agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct ttgaatttta
     7561 ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg cagatgattt
     7621 ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat atgacaatat
     7681 caagcggaca agcaagatct tggaggatta tgaaaaaaaa cttcatcttt tttctaatta
     7741 tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata agtacccgga
     7801 agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat aaataaaagg
     7861 aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc gatgggaaaa
     7921 ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg ggaaatccgc
     7981 cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac taagcgcgta
     8041 cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca acaaatcgta
     8101 aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga gttaaataaa
     8161 tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg taaaacgacc
     8221 tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc gctcctaatc
     8281 gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg tcaacagcta
     8341 gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt ccgtcaaggg
     8401 atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc aggacgtctg
     8461 catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac gcaaccaaat
     8521 gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt agctgatagc
     8581 ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg ggatactcga
     8641 ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt catcggttct
     8701 ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag ccgtatcctt
     8761 ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactatga cgagaaaaaa
     8821 gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga ctttattgaa
     8881 cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat gatcccagga
     8941 atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt tgaacgcaaa
     9001 aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct gttgaaccca
     9061 agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt gaatcgaatg
     9121 atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg ggatatgaac
     9181 atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg caaaatggcg
     9241 atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata agatgaaaaa
     9301 aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca gcaattttta
     9361 ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg tccgtggagt
     9421 gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc tgaatatgat
     9481 gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt gagctggcag
     9541 acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga tattaaaaaa
     9601 gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg aattattcgt
     9661 aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa aattcaagaa
     9721 gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat cgttgattct
     9781 tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga attagcaaaa
     9841 gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc ttttttagaa
     9901 gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga ggtcgtacgt
     9961 atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc tataagttta
    10021 gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac tccaacaggt
    10081 aagcaaacca cttatgaagc tttttatgaa acaatcgata cgaccatcat gggcagaaaa
    10141 acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga gaaaaagaat
    10201 ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt aaacgaagat
    10261 cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg ggttgtgggt
    10321 ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg gtatatccag
    10381 attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac atatgaagcg
    10441 cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca ttatgtacga
    10501 aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg tacatagttg
    10561 ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat ctagatacgc
    10621 atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat tttcatcaat
    10681 tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca cattcaaaaa
    10741 gacaagtgat tgtcgtagat ggtggtttgc gccaaagccg ccgatcccac ctggtgatgc
    10801 agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc ttgagcctac
    10861 atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag taacgaaaac
    10921 gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag gagcctgttc
    10981 tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa atacactttc
    11041 gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt aagaatcttt
    11101 tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc tccttcaaac
    11161 tatctataac aatcttacaa aaaataagta actggagcaa ataaaaacac ttactgacaa
    11221 aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa atcttctatt
    11281 tcctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca gggtaaaaaa
    11341 aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat aatggcagtt
    11401 aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat cgtcgtagct
    11461 gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta caatcctttg
    11521 aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt atcaaaaggt
    11581 gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat gcaaaaacac
    11641 cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta agcgacttag
    11701 tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta gacgtcgtag
    11761 agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt ggccgtatca
    11821 ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt gttcggatca
    11881 atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa ataaacgtgc
    11941 tgtactcgaa ttttcgagtg cagctttttt gtttttttat taattgaaga aatgattatt
    12001 tggcaaaaca agcaagaaag cgtatcctta taggaggagg gagtgaacag ccaatgagaa
    12061 agaaaatgtt atttattcca tttgctctat tgttaagcgc ttgtagttcg gttactcaag
    12121 aatcagacaa tacacatcta tcaagcagta tatctgaaac tgttaccagt ccagaaaaaa
    12181 gtagtaccaa aacggctacc accactgaac aaacaatggc tacctcaaat aatgagaaaa
    12241 agactgcttt agaccagttg aaagaacaac agccaaatgt tcccatgcct ctagatgtac
    12301 cagtctcttc aggctatctc aatatcgcag cgacacatac aaagcaaggt tattctattt
    12361 tatattacag aacagatcgt cccctcgggt tgaatgctga cgaattgaat caagaaacgc
    12421 ctattgcaac ctatctatat caatatgggt ttgcttctag tcaagaaaca atccaagttt
    12481 tgcagccctt tgagatcgac acaaatggtc agcaagtgga cttaggttct cgaatcacag
    12541 ggtatcagca aggtgcagca ggttcaagtt ttcttgaatg gcaagaagga aactggcgta
    12601 ttcgaatccg tgggaataat atagaaggcc aagatccctt actattagct aaagaaatcg
    12661 tagcttattt ggaagaaaac agtctaccag cacctgaaca atttggtaaa attactgttg
    12721 atatgggtga tacgacgaat cgagccgtag aagtaagctg gcaagaaccg aaaaatgcct
    12781 atacgatcac tcatcaagat cctatgtctg cgttgaagat ggcagtttct atgaagaggt
    12841 tgtgaaatca gcgcgctgat ctgagcccat catttaactc caagtcacag gaagtaatca
    12901 aaaatagcac gccgcatttt tgcataattt tggcttaata ccttaaaaga aaactagaat
    12961 tgcctcttca cacatgctaa aataaaacag acaaaaaaga aatggggata taagttgact
    13021 gaatacttga atgtaggaaa aatcgtaaac acacaaggta tcaaaggcga agtccgtgtt
    13081 atctcaacaa cggatttccc agaagaacgt tacaaaaaag gaacggtgtt aacactattc
    13141 caagacggaa aagcacctgt ggaattaaca gtcaaaagtc atcgcaaaca taaaaatttt
    13201 gacttattga gttttgaagg acatccttct atcaatgatg tggaaaaata tcgggatggc
    13261 atattaaaag tgtcaaaaga caactctgta gatctagctg aaaatgaatt ctattaccat
    13321 gagatcatcg gtctgcatgt attagaagat ggaactgagt taggtaaagt aaaagaaatc
    13381 ttgtcaccag gtgccaatga tgtttgggtc gttcaacgtc cgaaaaaagc tgatatattg
    13441 cttccatata tcgcttctgt cgtcaaagaa gtcgatttag aagctggagt ggtccgtgta
    13501 gaaattccag aaggactgat tgacgatgaa gattgatgta ttgactttat ttccaagaat
    13561 gtttgaaggt cctatggggg aatccatcat cggtaaggct gtagataaag gattattaga
    13621 tatcaatatc tctaacttcc gcgattattc agataacaaa catcagacag tagatgatta
    13681 tccatacggc ggtggagcag gtatgctgct caaagtccag cccatctatg ataacatcca
    13741 agccattgaa aaagctgcac cggatgtcaa aaaaagagta attcttcttg acccagctgg
    13801 taaacgattc gatcagaaaa tggcagaaga attttcacaa gaagaacact tagtattcat
    13861 ttgtggtcat tatgagggat atgatgaacg tatccgcaca ttagtgacag atgaagtttc
    13921 cttaggagat tatgtcctta ctgggggaga attaggtgcg atggtcatga tcgatgcaac
    13981 agttcggctt ttgccagatg tgttagggaa tcagacttca gctcaaacgg attcttattc
    14041 aaccggttta ttggaacatc cccaatatac tcgcccggca gaatataaag ggatgaaagt
    14101 accagaagta ttgacaaatg gaaatcataa attgattgaa gaatggcaat taaaagaatc
    14161 actaagaagg acctatcaac gtcggcctga tcttttggaa acacttgaat ggacacctca
    14221 aatgacgaaa tttttagaag aaatcaagaa agaagaacaa gaaaaaacag cagatgaagc
    14281 caagtgactt tttctaaaat attctttaca gtaggttgat aatatgttag tattttatgg
    14341 tgagtgcaaa agcacttaac tattacgata ttccgctatg agaaaagctc ataagaatat
    14401 ttggaataag gagaagaaaa ccatgaatcc attaatcgaa gaattaacaa aagaacaatt
    14461 acgttctgac atcccagcgt tccgccctgg tgacactgtg cgtgttcacg caaaagttgt
    14521 tgaaggtact cgtgaacgta tccagttatt tgaaggtgtt gttatcaaac gccgcggtgc
    14581 tggaatcagc gaaacttata cagtacgtaa agtttctaac ggagtgggcg ttgaacgtac
    14641 attcccattg catacaccac gtgttgcaaa aatcgaagta gttcgctacg gtaaagtacg
    14701 tcgtgcaaaa ttgtactacc tacgtgcatt acacggaaaa gctgctcgta tcaaagaaat
    14761 ccgtcgttaa tcattgttat cagactgaga aagaaccctt ggtattcaag ggttcttttt
    14821 tcttagcatg taatttgaac accctgatcc agtttttttc tttaattatt ggtaggggag
    14881 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa
    14941 tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag
    15001 tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaaaat
    15061 gaaagtttga gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa
    15121 aaagaaaaac atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac
    15181 agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca
    15241 aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca
    15301 gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat
    15361 gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa
    15421 agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag
    15481 ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat
    15541 gcgttacatg aataaagata attctctttt atttgtacga ataaatacca aaacgtacct
    15601 cctaaaatta gattcaaaga agatagcaaa gaggaaaaag atagttctgc agcagctata
    15661 gcggcatgtg gattgctaga actttccaaa caactgcctg tttcagatcc tcgtcatttc
    15721 gattacgaag agatagcaat caaaatcttg gaagaattac agaaaaacta tacaacgaaa
    15781 cgagttaacg gatcaaacgg tctgcttctc catgccgttt atgataaaaa cagcttaaaa
    15841 ggtgtagatg aatgtgtgat ctggggcgat tacttttatg tagaaggaat cactcgtcta
    15901 gctaaaacat ggtactgtta ctggtgatgt ataagagaag aggaggattc atataatgaa
    15961 gcgatggaaa aaaatgatga ttggatcaat agttttagga atgtcactcg tatttggtgc
    16021 ttgtggtaat caagaagaca gtagtgaaga atcagcaaat gaaattaccg tttcgacatg
    16081 gaattatgaa acaacacctg aattcaaagc gctctttgaa gcattcgaaa agaaaacggg
    16141 aatcaaagtg aaagcagtag atattgcttc agatgattat gatacgaaac tcaccacgat
    16201 gctttcttct ggagacacga cagatgtatt gacgatgaaa aatcttttgt cgtattcaaa
    16261 ttatgcttta cgagatcagt tagtcgatca aacagaacga attagagaat tagacactga
    16321 tgctgctgaa gaaacatatg aaatgtatga tatcgatgga aaaacatatg cattgccata
    16381 tcgaacggac ttttgggtac tatattacaa taagaaaatg tttgatgatg caggaatcga
    16441 atatccaaaa aatctaactt gggacgaata tgaagattta gcaaaaaaac ttagtaaaaa
    16501 tgacggacaa gtttacggag catatcaaca tatctggcgt tcgacaatcc aagctattgc
    16561 agctgctcaa aacgaggcga atcttgttga accagattat caatatatga ctgattatta
    16621 tgatcgagca ttgaggatgc aaaaagaagg cgcgcagatg gattttggca cggctaaatc
    16681 aacgaaagta acctatcagt ctcaatttga agaacaaaaa gcagcaatga tgtacatggg
    16741 aacttggtat atggctggca tactcgcaaa caaagaagca agtaagacag aagtagaatg
    16801 gggaattaca gctattcctc agaagaaaaa aggagaatcg gtcacgacgt ttggctcacc
    16861 aacagcattt gctgtcaata aaaactcaaa gaaacaaaaa gcggcgcaag aatttattga
    16921 atttgcagcc agtgaagaag gagcaaaagt attagcagga gtaggagtag ttccttcata
    16981 tcgtacggat gagattgatc agctttattt taatctcgct ggaatgccaa cggatgaaat
    17041 ttctaaaaaa gctttttctc ctgatgaaat caaacttgaa tttccgattg atacgtatgg
    17101 gcctgcaatc gataagattt tacaagaaga atatgactta gttttggttg gagatgaaac
    17161 gccggaaaaa ggaacggtaa atatggagaa acgtgtagct gcggaaaaag aataatcatg
    17221 aaaggagaga agccaagaga taaaacactc ttggtttcgt catcgaaatg gagaatagta
    17281 taaataagaa agctacaaat catttgcgta gaaaacgaac gtggatcgct tattcgttta
    17341 tcgccccgaa tttcattggt tttttcgttt ttacattaat accaatcctg ttttcgcttg
    17401 tactagcatt tatgaaatgg gattcttttt caacgccaga atttgctgga ctagataact
    17461 ttcgtaaaat ggccaacgat gagacatttt ggatttcttt gaaaaatact tttttatata
    17521 ccgctgctgt agtgcctggc acacttgttt gttcgttagg gctagcttta ctactaaatc
    17581 agaaaattag agggatgaag ctttttcgta cgatattttt ctttccgtat gttacttcct
    17641 tagtagcaat tgctgttgtt tggaatatgt tgtttcatcc aacagtggga cctattaata
    17701 tctttttaag acgatttatt gaacatcctc caggttggac ttcaagttca aattgggcgc
    17761 tttgggcaat tgtaattgtc agtatttggc gagggatggg ttattacatg attctatacc
    17821 ttgcaggact acaaagtatc caaaaagaac tatatgaagc gtcttcgcta gatggagcaa
    17881 accgttggaa acaatttttg aatgtgacat taccttcgtt gagaccaaca accttctttg
    17941 taacgatcat gcttgtcatc aattgtttca aggtgtttga ccttgtgcaa gtgatgacgg
    18001 atggcggccc tggtcgagcg acaaatgtgc tggtttacca aatctacaat gaagcattca
    18061 aaaaatttga ttttggttat gcttcagcta tttcacttgt tttgttcgta attgttttgg
    18121 gcgttacggt tgttcaattt tcttggaata agcgtcgtac ttaggaggtg tgatatggaa
    18181 gagagtgttc gaaatgaaag aaaaaaagca aaaattcgtt caaaaaacat gccctttatt
    18241 caatggttgg gtatgattct gctgattgta ttagctttat tgacgatcat tcctttttta
    18301 tggatggttt ctgcttcatt caaagcaaat aatgaagtgt taacgattcc gattcaatgg
    18361 atccctaaaa cttggcattt agagaattat ctggttattt ggtctcgtat tcctttagtg
    18421 acatttttta agaatacagc ttttttaagc gttgtgatca cgttgattca gttgtttact
    18481 tctagttttg ctgtttacgg gttttctgaa ctaaatttta aagggcgcga tattttattt
    18541 ttagcatata tcggcacgat tgctgtccct tggcagtctt atatgatccc gcagttcatt
    18601 atgatgagaa aagtgggttt aacagatacg ttgtggtcgt tggtgctatt acaagcgttc
    18661 aatgcttttg gtgtgttctt gttgaagcaa tattacagta gtatcccaga ttctttatgt
    18721 gagagtgcgc ggatcgatgg cttatcagag tgggaaatct atcgcaagat cattttacct
    18781 cttacaaaac cggcgattgc cagtttgacg atcatcacat tcgttaatac atggaacgat
    18841 tatatggggc cattcattta tttgtcttct acggaaaata aaacaatcca gttagggttg
    18901 aaaatgttta ttgggctatt tgatgcagag tacgcattaa tcatggctgc ctcagttgtt
    18961 tctgttcttc ctgttgcttt tatattttta gcgatgcaaa aatattttgt agaagggatc
    19021 gcttcatcag gaatgaagga ataggaggtg attatgaaag agcagcgttt tgacaaaaat
    19081 gtttatatgg aattctttta ttggttgtat caatcattgg caagtagcct ttgtttttgg
    19141 tgtgtcaata tcttgtatat agcagctgcg ttccttttgt cgatagatgg aaggaatgtc
    19201 ttttccttta ttttttcttt actttttatt ggtccaggtt gcatcgtgat gataggtatg
    19261 acagaagaat tagcaaaaga aaaaagtttt tcaccagtac gtttgttcat aaagacgttt
    19321 cgccaatttt ggctaaaggg atttttttat tggctatttg cttggatagt tagtgtgata
    19381 atgatttttg attgtttctt ttttatacgt ttttcttatg gaaaatggtt gattccgttg
    19441 tttgttttac ttgcttgttt tacttgcttg tttatcagtt tcctttagca tcaactgctg
    19501 gtattttcag gtgagaaatc cagcgtcgaa accaaatcaa gttctacgga tagcgttcta
    19561 ctatacgttg aaaaaatggt atgtgagttt actagatttt ttactattga caagtttgtt
    19621 tctcttcttt ttcgtaaagc cccaatggtg tattctacta ggtccttcta tcgtatttgg
    19681 tttgatttac ttcaacaatc gtaaattgat gagaactatg gatctgtaga ctttataaat
    19741 tgaaactttc aattaagaat gacagttttt tatgtaaaaa ctgtcattct ttttcatttt
    19801 ttgaaacata tggctaaaca aatgaaataa aacatgctat acttttgaat gaaacaggtt
    19861 tagaaactgt atcacttatt tgtgattatt tctaactaat aattaaaaat aaagcataaa
    19921 aaggacgatg ttcaatggct gaaaaaatga aacaaggttc gaaatctgta gtagaaagtt
    19981 tgattaatca tcatgttgat tatgtattcg gtataccagg tgcaaaaatc gatggtgtat
    20041 ttaatgaatt agaggatcaa ggtcctgaat taatcgtgac tcgtcacgaa caaaatgctg
    20101 catttatggc acaagcaatc ggacgtatta caggagagcc aggagtagtt atcgcaacaa
    20161 gtggtcctgg tgctagtaac ttagctacag gtttggtaac agcgacagca gaaggtgacc
    20221 cagttttagc aattgccgga caagtaaaac gaagcgatct attgaagttg acccatcaaa
    20281 gcatggataa tgcagcactg ttccagccaa tcacaaaata cagtgcagaa atccaagatc
    20341 cagaaacaat ttcagaagtg attgccaatg cttatcgtat ggctaagagt tcaaaaaaag
    20401 gcgcgagctt tatcagtatc ccgcaagatg tagtggatgc acctgtccaa ggtaatgtca
    20461 tcaagccatt gagcgatcct aaattaggtt ctgcttctgc agatgatatc cgttacctag
    20521 ctgaacgcat tagagaagca aaactaccgg tactattagt aggtatgcga ggatcaagtg
    20581 aaaaagaaac gttagccatc aggcaattag ttgaaaagac agcattgcct gttgtagaga
    20641 cattccaagc agctggagtg atctcacgtg aattagaagc gcatttcttt ggtcgtgtgg
    20701 gtttatttag aaaccaacca ggagatatgc ttttgaaacg aagtgatcta gttattgcaa
    20761 ttggctatga tccaatcgaa tatgaagcaa gaaactggaa tgcggaaaaa gatgcgcgga
    20821 tcattgtgat cgatgaagcg ccagctgaaa tcgatccttt tatgcagcct gaaagagaat
    20881 tgatcggtga tatctcagcc actcttgatt tattgacagg ttcattagag cctcaacaag
    20941 tgtcagaaga tgcaaaagaa tatttagcat ctcttcaagc aaaattaacg gagcgggaca
    21001 ttgttcaatc aaaaggagaa gcaggtatct tgcatccttt agaagttatt aacacgcttc
    21061 agtcaaaagt aacagatgat atgactgtta ccgtagatgt aggtagtcat tatatttgga
    21121 tggcacgcca ctttagaagt tatgaacctc gtcatttgtt attcagtaat ggtatgcaaa
    21181 cgcttggtgt tgcattgcca tgggcgattt ctgctgcatt ggtgcgtcca aatacacaga
    21241 tcgtatcagt ttccggcgat gggggatttt tattctctgc ccaggattta gaaacggcgg
    21301 tacgtaaaaa attgaatatc gttcatttga tttggaatga tggacactac aatatggtag
    21361 agttccaaga aaaaatgaaa tatcagcggg cgtcaggtgt agactttggt ccagttgatt
    21421 ttgtgaaata tgcagaagct tttggcgcta aaggaatccg agctacaagt gtagaggaat
    21481 tagaaaaagc tttagaagaa ggatttgcga cagaaggacc cgtaattatt gatattccga
    21541 ttgattaccg tgataatgag aaattgggag aaacaatctt accagatcaa ttctattaag
    21601 gaagtgtcga catgactgaa aaaatcttat accagcacgg gacattagga gctttgatgg
    21661 ccggcttgat ggatggaaca gaaacgattg ctcatatatt agaaaaagga actctgggat
    21721 tgggaacatt gcatgggctt gatggtgaag tcatcttttt agatggcgta gcttatcaag
    21781 gaagatcaga tggctcagtt gcccaattag atggaagtga acttaccccc tatgcagcaa
    21841 ttacagattt tacgccagat actagttttt ctgtctcaaa aactgcagat ggagaacaat
    21901 tgaaaaaaga catattagta agagaagccg gggaaaacct atttttggca gtgaagataa
    21961 ctggattatt caaaaatatg cacatccgta tcatgccgaa gcaagaaaaa ccttacagaa
    22021 gactagcaaa aatttcagag tctcaaccag aattccaaca aagcaatgtc caaggaacat
    22081 tagtcgggtt ctttacgcct gaattattcc aaggagttgc tgcagcggga tttcacctgc
    22141 attttatcga tgatacacat acatttggcg gacatgtaat ggattttgaa gtggctgaag
    22201 gaaaagttga gattagccgc atctcgtccc ttgtacagca ttttccagtt gatgatccga
    22261 cttttgttca cgaaacgatt gattatgctg atttagcagc agaaatccaa caagcagaat
    22321 agaaaaagac agcgagatgc gttcaaggtg attaacctga cgtatctcgt ttttttgagg
    22381 agaaaaccta gcgtttacaa aaaaactgag ctataatctt ctccatagat caaaaaggag
    22441 cggtttttat catgtggaca gaaaaacaga aaaaagatgc atacgaaatt ttattattac
    22501 agcaacaagg attattagaa gcagaagata actggttagc aaatttagca aattcatctg
    22561 cattgttgaa tgagactttg cctgagactg tctttgcagg atattattta ttcgatggta
    22621 atgaattgat tttaggaccg ttccaagggc gagtttcttg cacacgtatc aagatgggaa
    22681 aaggagtttg tggagaatca gctgaaaaac aagaaacatt gatcgttgat gatgtcaaaa
    22741 cccatgcaaa ttacatttcc tgcgattcag ccgcacaatc agaaatcgta gtgccaatgg
    22801 taaaagaagg taatttaatc ggtgttttag atatcgattg cggagtaacg aaagggtatg
    22861 atgaaatcga tcaagcattt ctagaaaaat atgtagatct tttattagca aattttaata
    22921 taaatgcttt ttcatttatt aataactaaa atcgtctatt gaaacaaaaa agaagag
//
LOCUS       DAYYME010000035        22930 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000035 DAYYME010000000
VERSION     DAYYME010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.035"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..366
                     /locus_tag="ACHBFW_001121"
     CDS             <1..366
                     /locus_tag="ACHBFW_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9317441.1"
                     /translation="FTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVGV
                     EDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLGE
                     EYVFNFADPDGNYFAVTGK"
     gene            546..1487
                     /locus_tag="ACHBFW_001122"
     CDS             546..1487
                     /locus_tag="ACHBFW_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFM9317442.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1556..2287
                     /locus_tag="ACHBFW_001123"
     CDS             1556..2287
                     /locus_tag="ACHBFW_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9317443.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2431..4611
                     /locus_tag="ACHBFW_001124"
     CDS             2431..4611
                     /locus_tag="ACHBFW_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFM9317444.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4631..5113
                     /locus_tag="ACHBFW_001125"
     CDS             4631..5113
                     /locus_tag="ACHBFW_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFM9317445.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5129..5890
                     /locus_tag="ACHBFW_001126"
     CDS             5129..5890
                     /locus_tag="ACHBFW_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM9317446.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6021..7355
                     /gene="murC"
                     /locus_tag="ACHBFW_001127"
     CDS             6021..7355
                     /gene="murC"
                     /locus_tag="ACHBFW_001127"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFM9317447.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7662..8147
                     /locus_tag="ACHBFW_001128"
     CDS             7662..8147
                     /locus_tag="ACHBFW_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFM9317448.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8140..8823
                     /locus_tag="ACHBFW_001129"
     CDS             8140..8823
                     /locus_tag="ACHBFW_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFM9317449.1"
                     /translation="MNNPSTLMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8949..11594
                     /gene="polA"
                     /locus_tag="ACHBFW_001130"
     CDS             8949..11594
                     /gene="polA"
                     /locus_tag="ACHBFW_001130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFM9317450.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11639..12475
                     /gene="mutM"
                     /locus_tag="ACHBFW_001131"
     CDS             11639..12475
                     /gene="mutM"
                     /locus_tag="ACHBFW_001131"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFM9317451.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12439..13068
                     /gene="coaE"
                     /locus_tag="ACHBFW_001132"
     CDS             12439..13068
                     /gene="coaE"
                     /locus_tag="ACHBFW_001132"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFM9317452.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13120..13452
                     /locus_tag="ACHBFW_001133"
     CDS             13120..13452
                     /locus_tag="ACHBFW_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM9317453.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13764..14255
                     /gene="nrdR"
                     /locus_tag="ACHBFW_001134"
     CDS             13764..14255
                     /gene="nrdR"
                     /locus_tag="ACHBFW_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFM9317454.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14278..15651
                     /locus_tag="ACHBFW_001135"
     CDS             14278..15651
                     /locus_tag="ACHBFW_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM9317455.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15651..16577
                     /gene="dnaI"
                     /locus_tag="ACHBFW_001136"
     CDS             15651..16577
                     /gene="dnaI"
                     /locus_tag="ACHBFW_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFM9317456.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16706..17146
                     /locus_tag="ACHBFW_001137"
     CDS             16706..17146
                     /locus_tag="ACHBFW_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFM9317457.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17290..17991
                     /locus_tag="ACHBFW_001138"
     CDS             17290..17991
                     /locus_tag="ACHBFW_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFM9317458.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17988..19109
                     /locus_tag="ACHBFW_001139"
     CDS             17988..19109
                     /locus_tag="ACHBFW_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFM9317459.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19124..20356
                     /gene="pepT"
                     /locus_tag="ACHBFW_001140"
     CDS             19124..20356
                     /gene="pepT"
                     /locus_tag="ACHBFW_001140"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFM9317460.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20488..21396
                     /locus_tag="ACHBFW_001141"
     CDS             20488..21396
                     /locus_tag="ACHBFW_001141"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM9317461.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21431..21703
                     /locus_tag="ACHBFW_001142"
     CDS             21431..21703
                     /locus_tag="ACHBFW_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFM9317462.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21714..22760
                     /locus_tag="ACHBFW_001143"
     CDS             21714..22760
                     /locus_tag="ACHBFW_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFM9317463.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         7733 a         3991 c         5022 g         6184 t
ORIGIN      
        1 tttacgaatg aaataaaaat catgctttat gtggacgatg tagaaaaaaa tgcaacgttt
       61 tggagagcag ttggctttgc ggaaaaagat cgtcaagaaa tggatgggac gcttataatt
      121 gaagtaggag ttgaggacag tcaaacctca tttctgttat atgatcgagc atttgttgag
      181 tcccattcac ctggcacagc tactagtgcc ccatctttga tgttttacag taaagatatc
      241 tttggactat ataaaaaaat gcagacgcaa tcagtcacat taggtgatat ggtacagctt
      301 ggagaagaat atgtctttaa ctttgctgat cccgatggca actactttgc ggttacaggt
      361 aaataataga aataaagact ttgtttttct gtgcgaaaca ggaaagcgaa gtctttttaa
      421 ttcaaataat aatattatgt aaacaaaaaa ctttccatgc gtaattatac acaagcaaat
      481 aaatcgtcaa atgcgaacgc cttctttata ctgtttagga acgaaaagaa ggaaggaaga
      541 aatacatgga ttatcaagta ctgctttatt acaattatac aaaaatcgaa gaaccagagg
      601 aatttgccaa aaatcattta gccttttgca aatctttgga tcttaaaggt cgtattctag
      661 tagctgaaga aggaataaat ggtactgtgt ctggaaccgt tgaaaacacg actgcctata
      721 tgaaagcaat gaaagctgat cctcgatttg ctgatacgat gttcaagatc gatccaagcg
      781 aaggacatgc ctttaagaag atgtttgtaa gataccgtcc agagcttgta agtttaaagc
      841 tagaagagga cattgatcca aaccaagtga caggtgccta tctttcaccg caagaattta
      901 aagaagctat tttagatgaa cagacagtgg tgattgatgc acggaatgat tatgaatatg
      961 acttgggaca ttttcgaggg gctgtacgcc cagatatacg cagttttcga gagcttccag
     1021 actggatccg cgagcacaaa gaagaattta tggaaaaacg ggtagtgacc tactgtactg
     1081 gtggcattcg ttgcgagaag ttttctggtt ggcttgtccg tgaaggattc aaagatgtcg
     1141 gacagctgca tggcggtatt gctacttatg gtaaggatcc tgaagtccaa ggagaattat
     1201 gggacggcaa gatgtatgtg tttgatgaac ggatcgctgt cgatatcaac caagtagata
     1261 aacaaatcgt cggtcgcgac tggtttgacg gaacaccatg tgaacgatat atcaattgta
     1321 gcaatcctga gtgcaatcgt cagattcttg cttccattga aaatgaagaa aaacatttag
     1381 gagcatgtag catagagtgt gcgaaacatc cgcgcaatcg ctatgttcaa gaccatggaa
     1441 tctctccaga agaagcagaa ctgcggttta cagctttaat taaatagaaa acaatagttt
     1501 tgattgtcga ccatttccaa tatgcgctat actagcgttg aggatggtga tccacatgaa
     1561 atcattgatt tatgctattg gggatgtcca tggcaaacat gagctttttg aaaaacttat
     1621 tcactattat gatccaaaga tccatcagct tgttttatta ggggatctca atgatcgagg
     1681 tcctcgtaca aaagaatgtt ttttattagg aaagcaatta gtcgaagaaa caggagccat
     1741 ttacctccgg ggaaaccatg aagaatattt tttgcaattt ctgcaatcac cggaggactg
     1801 gtatgcgcct tatatgagaa atggtggaaa agaaacgttt gaaagcctga ttcacccagg
     1861 ggcatcggag gaatactccc caactgaaat ctcgatgatg atccaatcga gatattcaga
     1921 actgattaaa tttttagtcc agcgcccctt gtattatgaa tgggaaaatt atctttttgt
     1981 ccatgccggt gtggatttga caaagaaaga ctggcgtaag acaagtctaa gagactttat
     2041 ttggataagg gaaccatttc atgaagggaa aaacaataca ggtaagacaa ttgtatttgg
     2101 acatacgatt acaccaatgc tccatggaga tatgcagaca acagacctct ggattcagga
     2161 tcataaaatc ggaatggacg gtggggctgt attcggaggg tcattgcatg gggttgtttt
     2221 tgacaaggaa ggtattcttc aagatattga atatcagaac aacgaaggac cttggcatgg
     2281 aatctgacta atggaaagaa tgaaggagct gtgactttgt tgcggctctt tttattttta
     2341 tagagaatat tgtttctttc agtagtttaa cccaggataa aaagtatgat agaataaaga
     2401 agcaattttt agcagaaaag aggcatttta atgacggaaa atttgaacca gacgatcatt
     2461 cagcttgtac aaaaagaact ttcagactat cgtcctaagc agttgactac tgtacttaat
     2521 ttgttaaatg aaggaaacac tgttcctttt atcgcgcgtt accgaaaaga aatgactgga
     2581 agtcttgatg aagtccagat tcgtgaaatc gaagagcggt atgcctatct tgaaaactta
     2641 gaaaaacgta aaactgaagt gattcggcta atcgatgaac aagggaaact gactcccgag
     2701 ttagaagcag aaatcaccca agccgtgaaa atgcaacaag tggaagattt gtatcgtcca
     2761 tacaaacaaa aacgacggac aaaagcaacg attgctaaag aaaaaggact tgaaccactc
     2821 gcgaagtggc tgatgcagct tacagatgga gaggtgcaat ctgaagcaga aaaatatatt
     2881 gacaaagaaa aagaagtctc ttctgccgaa gaagcgttgc acggtgctca tgagattatt
     2941 gctgaacaag tcagcgataa tgcaaaattt cggacttgga ttcggtctta tacgtataac
     3001 aaaggtatgt atgtcagcaa tgtcaaggat gagcaagcag atgaaaaagg cgtatatgaa
     3061 atgtattatg actttgcaga acccgtccat aaaatggttt ctcatcgaat attagctact
     3121 aatcgtggag aaaaagaaga tattctgaaa gtattctttc aagtagatga agcagcaatt
     3181 ttagcttact tggatcgcca aatagtcaaa aatcctgcct ctccttcttc ttcattcgtc
     3241 agagaagcct atcaggatag ttataaacgc tttattcagc cagcaatcga gcgagaactg
     3301 cgcaatgaat tgactgaaaa ggcagatgag caagcaatcg ctattttcgg cgaaaacctc
     3361 cgtaatcttt tgttgcaacc gccactaaaa ggtaaagttg tcctgggatt tgatccggct
     3421 tatcgtactg gatgtaaact agcagtcgta gatgcgacag ggaaagtttt agcaatcgaa
     3481 gttatttatc ctcataaacc tgcagcgcaa gcaaaaagag aagctgctgg accagctttt
     3541 atccaattaa ttaataaata tcaagtagat atggtagcta tcggaaatgg tacagccagt
     3601 cgggaatctg aactttttgt ggctgagcag ttgaaatccg ccgaccataa aacctattac
     3661 gctatcgtca acgaagcagg cgcttctgtt tactccgcta gcgaaattgc acgaaaagaa
     3721 tttccgcatc tacaagttga agaaagaagt gctgtttcta ttgcccgtcg cctgcaagat
     3781 cctttagctg aattagtcaa aatcgatcct aaagctgtcg gagtaggcca gtaccaacat
     3841 gacgtttctc aaaaacgttt ggctgaacaa ttggattttg ttgtggaaac agcggttaac
     3901 caagtaggtg tggatgtgaa tacagcaagc ccgcagctct tgcagcatat atcaggattg
     3961 aataaaacaa ctgcccaaaa cattgtcatt tatcgtgaag aaaacggaga attcaccgca
     4021 cgaactcaat tgaaaaaagt gccacgctta ggaccaaaag catatgaaca agcaatcgga
     4081 ttcttgcgag tccctggtgg gaaaaacatt ttggacaaca caggtatcca tcccgaaagt
     4141 tattctattg caaaagaaat attaacttca gtccaacttt cagaaaaaga gcttggtaca
     4201 gaagaagcag tagaaaaatt gacacgatta tccgtagaga aactggctga aagcctatca
     4261 gtcggagaag aaacgttagc cgatatttta gctgggctga cacaacctgg acgagatatg
     4321 cgtgatgaga tgccagcgcc cttattacgt acagatgtat tgagtatgga agacttgaaa
     4381 ccagggatgg aattgactgg aacggtccgt aatgtcattg acttcggcgc atttgttgat
     4441 atcggtgtga agcaagacgg acttgtccat atatcgaaac tcagcaaaaa attcgtcaag
     4501 catcctacag atgtcgtgtc agtaggagat atcgtaacag tttggataga acaagtcgat
     4561 acgaaaaaag gcagaatcag tttgacgatg ctttcccctt atgaagagta acaatgagga
     4621 gtaacaaaaa ttgacacaag aagaactgca gcagttagta gaagagatat cattaaaaag
     4681 cttccagctt cctttcaagc accaagcaat gttcaatcca cgcttacgta caactggcgg
     4741 gcgctatcat ttaaacgatc atcatttaga ttttaatgcg aagctatttg aagagtcaga
     4801 cgaagagacg attgcaggaa tcatcaaaca tgaactttgc cattatcatc ttcatctagc
     4861 tggaagaggg tatcagcata aagatatgga tttcaagtat cttttgaaaa aaacaggagg
     4921 gttgcgctat gcaccagcct tgacgaagaa acagataaag atggagtatt atcgatgcca
     4981 agattgttct gccacgatct gccgcaagag aagaatcgat gtacgcaaat atcgttgtgg
     5041 aagatgtcat ggagaaatca aatggattga gacaaaaaga gaagacttta atgaagcaca
     5101 aaaaatcaga tgaaaggata gaaggccttt ggaaaaaagt gagcagagga atctaaaaga
     5161 ctggaatcag ctattaaatt ttttagccgc acctgaagag ggaaattttg aggaactgac
     5221 ggcacctaca cttattttag ctggaaactg tcttcctatc ttagctgata aagcagcaca
     5281 aatgtattta aatggccaag tagatcaatt gtttttggtc ggaggggtgg ggcatgcgac
     5341 tagaatcctt tatgagaatt ttgaaaagca aggatttcat tttgaagatg gcatgagtga
     5401 aagtgaaatc tgtcgtcagt atctaaaaga agtctatgat ttgcccgata aggcttttct
     5461 gatagaatcg aagtctacaa attctggaga gaacgccact ttttcactgg agattcttca
     5521 ttccttagga gctgtaccag aaaaaatctt attgatgaat gatccgacac tgcaacgcag
     5581 gactcgagca acttttgaga aagtatggca aaatgaacaa actattttcg cgaatgctgt
     5641 gccatttgtg ccggaaatcc ttcacttttc tgaagagatc atctttacag caaaagaact
     5701 gaatcatcaa tggcctaagg aatatttcca tgcgctagta ttaggagaaa tggaacgtct
     5761 ccatgacgat gagaacggct atggtccgaa aggaaaagat tttattcctc acattgatat
     5821 atcagaggaa gtttggtctt cttacaccag aattaaagag tcaataaaaa cggatttttc
     5881 tcgtacttga ccattttagc aagcgaatcc tcctttccaa ataaatttgg ttgaaatttt
     5941 caatcgttta taagataatg aggagcgtgt tgaatgtaat agctaaaaga atggacgcta
     6001 gataagagaa gaggattcaa atggaaaatc aaaataaatt gtatcatttt gttggtatca
     6061 aaggttcagg aatgagctcc ttggctcttg ttttacatga acaaggtcta aatgtccaag
     6121 gatcagacat tgaaaaatat ttctttacac aaagagattt agaaaaagca aatatcacga
     6181 ttctgccatt taacgcagat aatgtaaaac cagggatgac gatcattgca ggaaatgcat
     6241 ttcctgattc acatgaagaa atccagcgag caaaagaatt aggtttggaa gtgattcgct
     6301 atcacgactt tattggtcat tttatccaaa attatacgag tatcgctgta acaggctctc
     6361 acggaaaaac aagtacgact ggactgctct ctcatgtatt gagtggtgtc cgtccaacaa
     6421 gttatctaat tggagatgga acaggacatg gtgatccgca agcggaattc ttttcatttg
     6481 aggcctgtga atatcgccgt catttccttg cctattcacc agactatgca atcatgacca
     6541 atatcgattt tgatcatccg gattattata caagtatcga cgatgtattt acagctttcc
     6601 aaacaatggc tggacaagtg aaaaaagcta tttttgcgta tggtgacgat gcttatttac
     6661 gcaaattaaa agcaaatgtg ccgatttatt attatggtgt cacagaaaac gatgatatcc
     6721 aagcacgaaa catcgaacgc acaacttctg gttctgcttt tgatgtctat catggggatg
     6781 aatttgttgg gcacttcact gtaccagcat tcggtaaaca caatatctta aatgctttag
     6841 gtgttattgc agtagcttat ttcgaaaaac tggatctgaa agaagttgca gaagaaatgc
     6901 tgactttccc aggagtaaaa cgtcgattca gtgaaaagat cgtagcagac atgaccgtag
     6961 tcgatgatta tgcgcatcac cctgcagaga tcaaagcaac gattgatggc gcacgccaaa
     7021 aatatccaga taaagaaata attgcagttt tccaacccca tacgtttacg cgtacgattg
     7081 cattgatgga tgaatttgca gaagcacttg atttagctga taaagtttac ttatgcgata
     7141 tttttggttc ggctcgggaa gaacaaggaa acgttaagat tgaagacctt ggagcgaaaa
     7201 tcaaaaaagg tggagaagtc atcaaagaaa ataatgtatc tccattgtta gattatcatg
     7261 atgcagtagt aatcttcatg ggtgctggag atgttcaaaa atttgaacaa gcgtatgaaa
     7321 aacttttaag cagcacgacg aaaaatgtac tgtaatacaa ttactgcagt gtccttataa
     7381 aaatagaaag agggggagtg gcaaaaatct tgccaagccc ccttttttgc aacttatgat
     7441 aaacggtgtt caaaagcttg catctgcggt aataagccca atcaaatcaa aaacatgaga
     7501 agctgttttt tcttggctgt tcttattatt tgatgcagga cagctttttc acgacctctg
     7561 taaactatag gaatctgtta aatcaaggga gcagaacttg ccttttacgc tacttttggt
     7621 acaataatgt atgattaaaa ataaactgga gggtttctat cttgaacata ggtaagccga
     7681 gaaaactagg aaaaacaata aatgaaatcg aggaaggcga ctcgttatcc ttaacagaat
     7741 ctattgaaga taaccagctt ttgctgtatt tgggattgac gaatgatgca aatcctttgt
     7801 atatccaaca tgactttgca caaaaaacag aatatcaaca gccaatcgtt ccatcagtga
     7861 tgctgatggg gatcatcaca agtgcaattt caaagcatct tcctggacca ggatcacatg
     7921 ttgtcaattt ttctgtcaat tttattgaac cggttttcca ttatgaaaca ctgacttttc
     7981 agtttgaggt cataaaagta gataagatga aggatgtggt gacgatttct gtagaagcgg
     8041 agaatacgaa cgaagatcgt gtgttggatg ctgtcgttat ggtccaaccg ccacaagttc
     8101 ctgaattgga aatagcaatc gaaagtgaag gtgaattgaa tgaataatcc aagtacatta
     8161 atgtcaggta tcaatcgttt ttatgcaaaa gtttatggat ttttaggtat gggacttgcc
     8221 cttagtgcag tcacttctta cttggctctt tgggtatttc ctttgcaatt agcaactttt
     8281 atcaataatt ttccactagg ctttgtgggt ctgtggatcg ttgaactgat tttagtaatg
     8341 gttctaggcg taaaagcaca aaagaatccg agcttagcaa tcggtggatt tattgcctac
     8401 tctcttttga atggcataac attagcggtt actcttgcat actacgatat tggaacagta
     8461 acgcaagctt tcttgacagc tactttgatg ttcatgggga tgtctgttgt cggagtaaca
     8521 acaaaaagag atcttagcgg ggttggaaga gcaggacagg cagctttaat cggagtgatc
     8581 attgcactag tcttgaacat cttcattttg agaagtagcg tagttgagct gttcatctcc
     8641 ctactgttag tcgtcatctt tgcagggatt acagcttatg ataaccaaag catccgtaat
     8701 atctatgtcc agacaaacgg aacagcacat agtggaattg ctgtattcat ggcattgaag
     8761 ctgtatttgg actttatcaa cttgttcttg gcgtttctga gaatctttgg acggaacaac
     8821 taaaaaagaa aaaaattcat cagttggggc tataccgaat tatttggtat ggtcccgctt
     8881 tttcttttcg cattccgcag gattctttgt tagaatgaat aagaacttta cagtaaggag
     8941 ttaagaagat gacaaaaaat aaattattac tcgttgatgg gaacagtgtg gcttttcgcg
     9001 cattctttgc gcttcacaat tctcttgaac gtttcaaaaa tagaaacggt cttcatacaa
     9061 atgcaatcta tgcattcaat acgatgtttg aaaatgttat gcaaaaagag caaccgactc
     9121 atgttctggt tgcgttcgat gcaggaaaaa cgacttttcg gacagaaatg tatgcggagt
     9181 acaaaggtgg aagatccaaa acacctggcg aattcaaaga acaaatgcca tatatacgtg
     9241 atttgttgac aggactaggg gtacagtatt acgaattgcc caattatgaa gcagatgata
     9301 ttatcggtac tttggcagaa aaagtagaca aagatcaatt tgatgtcgtc gtgctttcag
     9361 gtgatcggga tttgactcaa ttagctagta aagaagtaaa agtggatatc actgtaaaag
     9421 gtgtcagcga tatcgaaagc tatacaccag aacatgtagc ggaaaaatat gatgggttga
     9481 cgccaaaaca gattattgac atgaagggac tagccggaga tgcctctgac aatatccccg
     9541 gtgtgactaa aatcggtgaa aaaacagcta tcaagctatt gaaacaatat ggctcagtcg
     9601 aaggaattta tgaacatatc gatgaaatga agcaaagcaa aatgaaagaa aatctgataa
     9661 atgacaaaga gcaggctttc ttatcgaaaa aattggcgac catcaatact tcagcgcctg
     9721 tggatgtctc tatcgactca ttgaagtatg aagggaaaaa tctcgataaa ctcgttccgt
     9781 tttataaaga aatggatttc aatcagttcc tttctaaatt gaatatcgta gaagaagaag
     9841 taaaaatgga tgatattttg tttgaagtcg ttcatgaatt caaagaggaa atgtttacta
     9901 cggatatggc gttgtacgtg gaaatgatgg gcgacaatta tcatacagag gaaatcgtcg
     9961 gagtagcgtg ggggacagaa aagaaaattt atgtcacgaa cgatctatct gtattcgaaa
    10021 accgagcgtt ccattcttgg atcactgatc cgactcggct gaaaaaagta tatgatgcga
    10081 aacgaactta tgttgcttta aaccgctata ctggaaaaac gaaaggaatc gattttgatg
    10141 tcttgctagg tgcatacctt ctcgatacga atgaaaaaag cacagatatc gaaggaatag
    10201 ctgctcatta cggatacaac gatatccaat cagatgaagc tgtatacggc aaaggggcga
    10261 aaaaaggttt accagaagaa gaggaactct tttttgctca tttagcgcga aaagtagctg
    10321 caatcaatgc gttgacacca aaattgagcc aagaactggc agaaaaaaac caagaagatc
    10381 ttttccggaa aatggaactg ccattgtcta tcattttggc agagatggaa ataagcggga
    10441 tcaaagtaga tgcgactcgc ttacaagaaa tgaagggcga gttctctgct cgcttaagag
    10501 agatcgaaca aaaaatctac gaagaagccg gcgaagaatt caatttgaat tcaccaaagc
    10561 aattaggagt gatcttattt gaaaaaatgg gattacccgt tatcaaaaaa acaaaaactg
    10621 gatattccac agccgtagat gtactggaac agcttcgtga acaagcacca atcgtagagg
    10681 atatcctcac ctatcgtcaa attgcaaaaa ttcaatctac ctatgtcgag ggactgctga
    10741 aagtgatcca atcagatgga aaagtgcata cccgttatgt ccagacattg acccaaacag
    10801 gacgattaag ctctgttgat cctaatttgc agaacatccc gattcgatta gatgaaggga
    10861 gaaagatacg tcaggcattt gttcctagag aaaaagactg gctgatttat tcttctgact
    10921 attcacaaat cgagttacgc gtacttgcgc atatctcaaa tgatgaacat ttgaaagctg
    10981 ccttcctaga aggtcaagat atccatgcca gcacagcgat gcgtgtattt ggaatcgaaa
    11041 aggcagagga tgtcacacca aatatgcgta ggcaggcaaa agcagtgaat tttggtatcg
    11101 tgtatggcat ctctgattac ggactttcac aaaatctagg aatcagccga aaagcagcac
    11161 agcaatacat cgatacgtac tttgaaaaat acccaggtgt caaagaatat atggaaagaa
    11221 tcgtacgcga agcaaaagac caaggctacg tcgaaacgct gtatcaccgc cgtcgatatc
    11281 tgccagacat caattcaaga aattacaata tccgttcttt tgcggaacga acagctatca
    11341 atacaccaat ccaaggaagt gctgctgata ttttgaaaat cgcgatgatc gaattggata
    11401 aacgattaaa agaaacaggt cttcaagcta cgatgctgtt acaagttcat gatgaacttg
    11461 tcttcgaggt accggaaaaa gaacttgaat cattagataa attggtgaag gaagttatgg
    11521 aacaagctgt ttctcttcat gtaccactga tcactgacag cagttgggga aaaacgtggt
    11581 acgaagcaaa ataaacattg tttaagacag agcaatataa tgaaggtgaa aagaaaaaat
    11641 gccagaatta ccagaagtag aaaccgttcg taaaggattg gaacggttag tagtaggaaa
    11701 aacgattcaa aaagttcagg tcttgtggcc caagatcatc gagcaaccag agacacccat
    11761 ctttgaggct tctcttgttg gcgaaaccat ccaatccatt ggacgtagag ggaagttttt
    11821 aatttttcat ctcgatcatt gtgaactgat ctcacacttg cgaatggaag gaaaatatca
    11881 atttacgaaa gaaaatacac caatcgataa acatacgcat gtacttttct tttttgagga
    11941 tggaagccaa cttcgctaca acgatgtacg aaaatttggt cgtatgacaa tcgtcgaaaa
    12001 aggagcaagt gcaacttacc gaggaatcat gaaattggga cctgagcctt tgccagatag
    12061 cttcttatta gcagatttcg caaatggact gaaaaagtcc cacaaagcaa tcaagccatt
    12121 gctgctagac caacgtctag tgactggtct aggtaatatt tatgtagatg aagcattatg
    12181 ggaagcgaag atccatccag aacaaccggc gaacacactg cgccccaaag aggtagaaca
    12241 attacgtcta tcgatcattg atgttttgga tcgtgcaata gaagccggag gtaccacgat
    12301 tcgcagttat ttgaatgcat taggagaatc aggcggattt caagtagcac tgcatgtcta
    12361 tcagcaaact ggcaaaccat gtatacgttg tggaacgccg atcgtgaaaa caaaagtggc
    12421 acaaaggggg acgcactatt gtccgaattg tcaaaggcta aaaatcgcaa agtaggattt
    12481 gttttaggtt tgacgggtgg aatagctacc ggtaaaagta cggcagccaa agtctttcag
    12541 tctcatggat ttcctttgat cgatggcgat gtgattgcca gagaaactgt tgagcctgga
    12601 acgcttgctc tgaggaaaat tgtatcagtg tttggccaag aggtattgca gcctgatggg
    12661 caactagatc gagggaaact tgggatgatt gtttttccat caaaggaaaa acggcaaaaa
    12721 cttgatcagc tattagatcc gtttatccga aaagcgataa aagatcaaat cgcctctcta
    12781 agttccaatc atcctttagt aattgtagat atcccattgc tctacgaagg tcattatgac
    12841 cactatatgg atgcagtagc tgtcgtttat actacgccag aaactcagct tcagcgtttg
    12901 atgaaacgta atcaactgac gctagaacaa gcgcagcaga gaattagtag tcaacttcca
    12961 atcgaagaaa aaaagcagcg tgcggatatt ttgttcgaca acaatggaac aaaagaaata
    13021 cttgtgggac aaatcgaaaa ttggcttttt gctaatcgct ttctttgagt atctgtgtca
    13081 attttgctat cctgagtata tagaaagtga gggatagcga tgggatcatt aaaaaatcaa
    13141 aaggataaag tcgtaggtaa cgcaaaagaa aaaatcggcg agatgctcaa taaagaaaat
    13201 ctaatcgatg aaggtcgtac gcaatcagaa acagcagaag aaaaagaaaa gcagtaccaa
    13261 gcaagaatcg atgaaataga agaccagcag gcagaagaag aagcgaagcg gcaagatttg
    13321 actcaagaac gaaaaaatcc gacacctgat cgtagtaaaa caccgctccc agaaagtgac
    13381 cacattcccg atgagcaaaa aatcgaggaa cagcgaatga aacactatac aggaatcgaa
    13441 gagaagttat aaaatagtaa aaaatcgaga tgtgacttga agcagatgag aaggtgttca
    13501 tctgcttcaa gttttttgtg ctagatgact aaatcagctt ttgtgtaagt tatttctcag
    13561 taatcaaggt gatcagtatt ggaaaattga aatttagtaa ggacagggga cacaattacg
    13621 catgaaagta gaaaaaattg ttcttatatc cgtgtttttt gggtaggaca gtcgcgtttg
    13681 tgatttgttt cgtgttataa tacaactagt tgtaaagaaa gacacatcct ttatataaaa
    13741 tgcgatgcaa gaggtgagac aaaatgagat gtcccagatg tcaacataat aattcacgag
    13801 taatcgacag ccgtcaagca gatgatggac gtgcgatccg tcgccgtcgc gaatgtgaga
    13861 attgttcgtt ccgattcact acatttgaac gaattgaagc tgctccgtta ttagtaatca
    13921 agaaaaacgg ggaacgcgaa gagttcaatc gcgataaaat tttaagagga ctcatccgtt
    13981 cagcagaaaa aagacctgta gctatggaac aaatggaaca aatcgtcaac catgtcgaaa
    14041 accgcgtacg cagtctcgga gaaaacgaag tatcgacgaa tctaattggt gaatatgtga
    14101 tggaagattt agttgattta gacgaaatcg cttatattcg ttttgcaagt gtttatcgcc
    14161 agttcaaaga tatgtctgtt ttcttaaaag agttgcaaga tatcgtggat aaagcaaaag
    14221 aaaataataa agagaataat aaagaaaaca attaagaaac aaaaaggagg catttccttg
    14281 gaaaatgctt ggaaagagct tcaaccaaaa aatatttacc aaggaagaaa agcggctccg
    14341 ctgagagaag acagcaagaa cgccttgctg tatctttatc agccactaat cggcggagaa
    14401 gccttgagta tctatttcac cctgttatca gaaatctctc aagaatctgg caaaggtcct
    14461 gaagggatgc atgcagatct gcttagtagt ctaggctgcg gtatcccgca attttatgat
    14521 gcaagaaaaa agctagaagg aatcggctta ctggatgttt attttaaaga agatagtgat
    14581 ctgggacctc gttttattta tgagctgtta gaaccactca ttcctaaagc atttttcaaa
    14641 gactctgttc tggcttttct tcttctggaa aaagttggtg aacgacgatt ccagaagctt
    14701 acccaattct ttgaacctaa aaggcataca gcagagggat atcaaaaaat cacaaaaaaa
    14761 tttttagatg tttatcgctt caatgaagca gtgtacgctg ctaatcaaga aaacatcgaa
    14821 tcgctgaata aatcttttga agaatctgaa tcagtagcat tacttgaagc agattcgaca
    14881 ttcgattggc agtttttgaa tgattggctg gcaaaaaaac atattgcaag tcttgatgat
    14941 ccaatcatca aacaagtcga catgtatcat cagctttacg gtttcgatga attgacactt
    15001 ggcgaactga tcagtcaatc ttatgacttt agtgaacaaa aagtatctga aaaagaactg
    15061 caaaaagcca ttttggcaat gcagagacgt ccggtgaagc aaacaaaaga aacagttgcg
    15121 gtttctgaaa acagtaatga accctcgtca aacgaacatt tgacaaacgc agcgcttcag
    15181 ctgataaaag aagcacagag cattccaccg atgaaatatc tggaagcaat caagtcagaa
    15241 aaaggcggat atgtttctaa acaagaaaac tggttactgc aagatcttgt ttctcaatca
    15301 ggtctttcca gtagcgtgat caatgttttg ctgaattatg tactagtgat caagaataat
    15361 gctagcttaa atgccagtta tgtaaataca gtggcaaatg aatgggcaca aaagaaaatc
    15421 gccacagcag aagaagcgat ccagcacatt cggcaaataa gtaatcaagc aaaacaatca
    15481 aaacaaaaga agcaaacaaa ttatcaaact ggcaagcgaa atgttcgacg ggaaaagctt
    15541 cctgattggg tcaaccagcc aaaagatgaa aaacagatca gcagcgagaa aaaagcagag
    15601 attgaccgcc gttttaaaga atacctggca aaaaaggagg gtgatatcta gtggaagacg
    15661 taggcaaaga attgaagagg atcatcagca aacgcaatta tcaagaaaag tatgctgaaa
    15721 tgatcgctga agtcttaaat gatgcggatg ttcaagcttt tctaagcgca caccaagaag
    15781 aattgacaca agcggatatt gaaaaaagtt acgctaaatt gtatgaattc gtgcaagaaa
    15841 agcgtaaata tcaaaaaaac gatccaacga tgatcgctcc cggctatgag ccgaagctga
    15901 cgttgaattt tcattatgtg gatgtcactt atgtaccaac ggaagcattg ttagcacggc
    15961 aaagacaaga agaaatcaga agccgagtaa aagcaatgga tatgccaaaa gatattcaat
    16021 cggcttcttt tgaaaacttt gaacgtacag atggacgagg atatgcgagc ttagaagccc
    16081 tagattttgt agagaaatat actgctgatc caaaagggta tcataaagga ctgtatctag
    16141 taggaagttt tggtattgga aaaacatatt tattaggagc aacagcgaga gaattagcga
    16201 ttgctggatt caccacgacg ctagtccatt ttccgacatt tgccgttgag atgaaacagg
    16261 caatcggaaa agatcaagtg gcagaaaagt tagatgcagt gaaacgttca cctattttga
    16321 tgatcgatga cattggtgca gatgcgatga gtagttggat acgagacgaa gtattcggtg
    16381 ttatccttca atatcggatg caggaacagt taccaacctt cttctcatcg aattttacaa
    16441 tggatgaatt ggaacagcat ttgtccgtca ctcaacgtgg agatgaagag ccgctaaaag
    16501 cgaaacggat aatggaacgc atacgttatt tgacaaaaga aatcgaaatg actggtcgca
    16561 accgacgcaa tggttgaaca gttgacaagt ccaactgaca tttgacctag tagtaaaagc
    16621 aggtcagcga atcattatat ggacagaaat gaccggagac aatcaccgca acaagtaatg
    16681 cagaaactta aacggaggat tttttatgag ggctattttt tctagtatat ctgtagttgg
    16741 tctatttttt cttttagcag gctgcggtag cgcagatagc aacacaggtt ctgttgattc
    16801 gattgatgcg ggtcaaacag taaaaacaag cagtacgaaa acggagagac aaagcatatt
    16861 tgtcggaacg attgaaagta tcactcaagt agaagcagaa gaagaaacgc ttcagttgaa
    16921 actcgtcgat gtcatggcca aagaagatcc agcgaatatg ggaacgtcat tcagcaatga
    16981 cggagtgatc ttaaatgcta ctgctgaaaa aataagcgtg gacataaatg aattgaaaat
    17041 cggtgacaaa gtccaattta cattagttga gatgccgatc atgacgatgt ctatcccacc
    17101 gcaagttcct ggagattcga ttattgatgt ttcattgaac aaataaccaa taacattttt
    17161 tcaaaaataa gaaaatgttg ttttatagaa cgaaaaggta tattttttaa ggagatatgg
    17221 gaattttttt attcctagtc tccttatttt atttggaaaa tgctataatt atctagaagg
    17281 agtgaagcta tgaaccattt agatttatct cagcgcttag ccgcagtggg tgactttgtc
    17341 cctgaatccg ctcgtttagc agacattgga tcagatcacg cttacttacc cgttgcattg
    17401 atattaagag gaaaaatcga atatgcagtt gctggtgaag tcgttcaagg tccttatgaa
    17461 tcagcacgta agcaagtcaa aaaagatgga ttagaagacc gtatcaccgt acgccttgca
    17521 aatggtctag atgctatcca gccacaagat ggtattacag ctgtcacgat tgcgggtatg
    17581 ggaggttcac tgatccgaga tatcctagaa gctggcaagt tgaatcaacg cttaagcgga
    17641 caagaacgtt tgatccttca gccaaacatc ggagagcgta cattacgatc ttggctgcaa
    17701 gaaaatcagt ataaaatcca atcagaaaaa atacttgaag aaaataaaaa aatatatgaa
    17761 atcattgtag ccgaaaaaga acaaaaacca gttacttaca atgaaatgga gctgatgttc
    17821 gggccatttc tgcttgaaga gaaaagtgag atcttcaaat caaaatggac aagagagctt
    17881 caacaaagaa aagctatccg tgaacagctc aaacaagcag ctaatcaaga ggcacgtcaa
    17941 aaacaaatcg agcaggaaat ccaaatgatt gaggaggttt taaagccatg acaatcagcg
    18001 gacgcacttt catccaaaag tttgaaagct attgtcctca gtggctagct gaagaaggag
    18061 acccagtcgg actgcatatc ggaacattgg ataagccaat ccaacgagta atgatgacat
    18121 tagacgttcg tccagaagtg gtcgaagaag caatcaagaa aaaaatcgat ttgctgattg
    18181 ctaaacatcc tccaatcttt cgtcctgtaa aacggctggt tactgatcag ccacaagaaa
    18241 aaatgtatgc agatttgttg aaacatgata ttgccgtcta tgcagcacac acgaacatgg
    18301 atattatttg ggatggactg aatgactggt tttgtgaact tctcggtatt gaagtggaga
    18361 gttatttagt aaagactcat gagattcatt ataaaaaact ggccgtttat gtacctgtgg
    18421 atcatgcaca gaaaatgcgt gaagtattag ctgcagctgg tgctggtaca caaggtgatt
    18481 ataccggtac aagttttacc tcgattggac acggtcgatt tactccggaa gctggtgcac
    18541 aaccagcgat tgggaaagta gggaagacag aacaagttca agaggcaaaa gttgaggtta
    18601 ttttgccgga aactatcgaa aaacaagtga ttcaagccat gcggtcagct catccttatg
    18661 aggaacctgc ttatgatctt ttcgctatag atgaaccagt cgaaatgttt gggttaggac
    18721 gagtaggtga attgccgcaa gaaatttcta tagaggcatt tgtagaacaa gtaaaagaag
    18781 cgttccaact agatggactg agaatagtcc agccaaaaaa tgcaaaatcc tctgtgaagc
    18841 gcattgcgat ttgcggtgga agcggtgaaa aattttatcc gcaagcaatc gcccaaagag
    18901 cagatgtcta tatcacggga gatatctatt atcatacggc tcatgatatg caaagcgctg
    18961 ggttgattgc aattgatccc ggtcattata ttgaatcgtt atgcaagcaa cgatttatcg
    19021 agaaatttga atcttggaaa caagaagaaa attgggatat taactttttc gtttctgaaa
    19081 caaatacaaa cccatttcaa tttaattaaa aggagagaat actatgtacg aaaatctttt
    19141 gccacgtttt ttacgatatg tcaaaacaga aacacgctcc gatgcaacaa gcaaaacgac
    19201 tccatcgact cagacacaag ttgcttttgc gcaagaattg aaaaaagaat tagaagagtt
    19261 aggcttagct gatgttttct ataatgaaga taacggattc gttatgggaa cattaccaag
    19321 caatgttgat catgaagtgc gttccattgg atttatttcc cacatggata cagcagattt
    19381 taatgcagta ggagtcaatc cacaaatcat tgaaaactat gatggcgagt ctacgatcgt
    19441 actagatgcg gaagaacgct atacattgaa tacaaaagat tttccaaatt tgaaaaatta
    19501 tgaaggccac acattgatca caacagatgg cagtacatta ctgggagcag atgacaaatc
    19561 agggattgcg gaaatcatga ctgcaatgga gatcttgatc aataatcctt ctatccctca
    19621 cggtaccatc aaagtggctt ttggtcctga tgaagaaatc ggtgtcggag cggataaatt
    19681 cgatgttgag caattcgatg tagattttgc ttatacagtc gatggtggtc ctttaggtga
    19741 gttgcagtat gaaacgttta gtgctgctca agcaaatatt acgatccaag ggaaaaatgt
    19801 acatccaggg acggcaaaag atacaatgat caatgctcta cagttagcaa tcgattttca
    19861 taatcagctt ccagccgatg aagttccaga aaaaacggaa ggatacgaag gtttcttcca
    19921 tttgatggca ttggatggga atcctgaaga agcttctatg agttatatca ttcgcgatca
    19981 tgatcgtgaa aaattcgaag cacgcaaagc acaaatcaca catatccaag aagagttgaa
    20041 tcagcgattt gatcaagaac gaatcaaagt agatatgtat gatcaatatt acaacatgaa
    20101 agaaatcatt gaaaaagata tgagcatcgt ggaattagcg aaacaagcaa tgatcgagct
    20161 tggtatctct ccagtgatcg aaccagttcg tggaggaaca gatggttcaa aaatctctta
    20221 catgggtatt ccgacgccaa atctatttgc cggaggagaa aatatgcacg gacgattcga
    20281 atttgtttct ttacaagtaa tggaaaaagc aacagacgtg attgtaaaaa ttgcagaatt
    20341 gaacacaaag agttaaaatc gaaaaagaac agcttatgac aaggcgttcg tcccaatgat
    20401 ggaatgtcat ctgtctaagg ctaaagggca ggacaatgga attggttgtc ccgccctctt
    20461 ttttgaagag taaataggag gtaaagaatg gaaaagctgc tagtttttta taacaaaagt
    20521 tcagggaaag atgagggtga gaaattagca cgctggtttc aagattatgt aaaagaaaag
    20581 cagccagaat tatatgtaga actagctgaa actggcccat cgattgatga tgcggaatta
    20641 gtcaagaaag cagaagccat agaagctgat actttagtaa ttataggtgg agacggcacg
    20701 attcatcata tcgtccaagc gttccaaaaa acattatctc aataccatgt cggattactt
    20761 ccaggtggaa ctgtcaacaa ccttgcacgt gttcttgaga ttcccctcaa aaaagaagaa
    20821 gctgctgacg tgatcctgga aggcaatgta cggaaaatag actatggtca agtcaatgat
    20881 gaagtcatta tctctacact gacaatcgga atcttagcag atacagcagt ttggatcagt
    20941 caagaagaaa aacagcgtta cggctcgtgg atctttatca agaagttttt taagttattg
    21001 ctgaagaaga aaaaatatca tttggattta gaaacagacg aggagcattg gaaaggaaaa
    21061 tcccaactgt tgacagtcac gatgtctaac tccgtgggcg gttttacaaa ttttgacgac
    21121 tcagcgaccc tagatgatgg aaaatttcat gtaacgatcg taccttcctt agatattttc
    21181 cgatttattt tttatttgcc tagaatcatg agaggcaaat tctatacgat ccccggcatt
    21241 acttatttca cagccaaacg catgaaaatc caagcgcagg aaaaatctgt gggcactcga
    21301 acggatggag acacgacaga tgatcttcca gttgaattat ctgttgtatc aaaaggatta
    21361 acagtttatt caccaaagaa agaagaaaaa gcttagaaat agtagttaac agaaagttgg
    21421 aagaagagta atggatgtat tgttacacaa tcctttgtgg ttttaccttt tcattgtgaa
    21481 tttttactta ttttgtctga tggggtatga taaacatcag gctagaacta gcggctggcg
    21541 tgttcctgag aataatttga tatttgtcag cctgttaggc ggtggtttgg gaggattgat
    21601 ctctcgaaaa gttttccatc ataaaactcg gaaaaagaag tttactatag gattcgctgc
    21661 aggtgtactt gttgatttga ttctcattta ttttttccat taaggagcag gagatgtcgt
    21721 ctaaaggaac gaaaactaaa atcattctga atctgctttt gctgattgtg atttttgcca
    21781 ttatttatta tttaatcaat caatcatttg cagatatctt tgccgaattg ctgtctacca
    21841 gtgttgttgt tttggcagcg gctgttatat tagggacagt ttaccaaatc gctgaagggc
    21901 gctcaatcaa agaaattgct gaacctttcg cgccgaattt tacaacgaaa gatgggttct
    21961 tgacctctag ctatattgcg ttttaccgaa tcgtttcgtt tggtacagga acgctgatct
    22021 cggagattta tttttataac aaaaaaggaa tgaagctatc ccagggagca ggggtgacag
    22081 cactccatat gattatgtac aaactggcag tgatcactta tgcattagct ggcttggtca
    22141 tccaattctc tcttttttat tctaatggtc catcaatgat ctggtttatc atcgctggta
    22201 tccttttgac gatcgtgatt gttgcctttt tattggcgat gtccgtcagc ttgaatgttc
    22261 aaatattttt tgtcagctta tcgaataagt tatttaaaag tgagcgtttg cggaaatggg
    22321 tggatacatg caatcagcag atctactctt taagggaaac ggtccaaacc attatccaag
    22381 acaggactgc gctattgcgg atttatctat ggaatatgtt aaaacttgta ttttggtatg
    22441 tacttcctta tttgattctg attgaaaacc atcctaaaat agattttcta ctgaccattt
    22501 cgtttatcag ctttgcagta gtgctttcag gggttattcc tacgcctgct ggtattggtt
    22561 cctttgaatt tgtctatttg ttattattca gaccagttgt tggaacagtt gacgcagttt
    22621 cttcgctttt actgtatcgt ttcagttcct ttatcttgcc atttctctat ggatttgtgt
    22681 atgtgattgc tgaacggcga agagtgatca aagaagaaat aatagaagta aaaaaagaga
    22741 ataaagaaaa tcaagggtag gtcttttata agatctaccc tttttatcaa atcaaagaaa
    22801 aacgttatgg ataagataat tttacgtttg tttggctctt tttttatata catacaatca
    22861 attttgtttt tttaagattt atataagtcg agaaaaaaag tcaaaaaact atgtgtatat
    22921 tttgaccaaa
//
LOCUS       DAYYME010000036        22254 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000036 DAYYME010000000
VERSION     DAYYME010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.036"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..436)
                     /locus_tag="ACHBFW_001144"
     CDS             complement(<1..436)
                     /locus_tag="ACHBFW_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM9317464.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEH"
     gene            complement(449..859)
                     /locus_tag="ACHBFW_001145"
     CDS             complement(449..859)
                     /locus_tag="ACHBFW_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9317465.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1134..1346
                     /locus_tag="ACHBFW_001146"
     CDS             1134..1346
                     /locus_tag="ACHBFW_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFM9317466.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1349..1561
                     /locus_tag="ACHBFW_001147"
     CDS             1349..1561
                     /locus_tag="ACHBFW_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317467.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            1701..1856
                     /locus_tag="ACHBFW_001148"
     CDS             1701..1856
                     /locus_tag="ACHBFW_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317468.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(1853..2077)
                     /locus_tag="ACHBFW_001149"
     CDS             complement(1853..2077)
                     /locus_tag="ACHBFW_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM9317469.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2155..2343
                     /locus_tag="ACHBFW_001150"
     CDS             2155..2343
                     /locus_tag="ACHBFW_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317470.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2482..2610
                     /locus_tag="ACHBFW_001151"
     CDS             2482..2610
                     /locus_tag="ACHBFW_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317471.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            2669..3004
                     /locus_tag="ACHBFW_001152"
     CDS             2669..3004
                     /locus_tag="ACHBFW_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317472.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3237..4178
                     /locus_tag="ACHBFW_001153"
     CDS             3237..4178
                     /locus_tag="ACHBFW_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFM9317473.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4180..5070
                     /locus_tag="ACHBFW_001154"
     CDS             4180..5070
                     /locus_tag="ACHBFW_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFM9317474.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5113..5913
                     /locus_tag="ACHBFW_001155"
     CDS             5113..5913
                     /locus_tag="ACHBFW_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM9317475.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            5928..6779
                     /locus_tag="ACHBFW_001156"
     CDS             5928..6779
                     /locus_tag="ACHBFW_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9317476.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            6980..7441
                     /locus_tag="ACHBFW_001157"
     CDS             6980..7441
                     /locus_tag="ACHBFW_001157"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9317477.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7467..7919
                     /locus_tag="ACHBFW_001158"
     CDS             7467..7919
                     /locus_tag="ACHBFW_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317478.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8025..8219
                     /locus_tag="ACHBFW_001159"
     CDS             8025..8219
                     /locus_tag="ACHBFW_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317479.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8250..8636
                     /locus_tag="ACHBFW_001160"
     CDS             8250..8636
                     /locus_tag="ACHBFW_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317480.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQKIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLSAFAQWGLEQEEAE"
     gene            8633..8836
                     /locus_tag="ACHBFW_001161"
     CDS             8633..8836
                     /locus_tag="ACHBFW_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFM9317481.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            8833..9129
                     /locus_tag="ACHBFW_001162"
     CDS             8833..9129
                     /locus_tag="ACHBFW_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317482.1"
                     /translation="MKIKDGFYASSHGIGGLMLDMPIKNPKTRQKSKFKIGDMVRCKA
                     EEFIYPFRGYVEHVYNHSAIIHIENTMECDKWLAKSKDNLAVARLVDMELINDK"
     gene            9205..9621
                     /locus_tag="ACHBFW_001163"
     CDS             9205..9621
                     /locus_tag="ACHBFW_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM9317483.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            9882..10019
                     /locus_tag="ACHBFW_001164"
     CDS             9882..10019
                     /locus_tag="ACHBFW_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317484.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQLQETLDEIANFKIQV
                     S"
     gene            10429..10659
                     /locus_tag="ACHBFW_001165"
     CDS             10429..10659
                     /locus_tag="ACHBFW_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317485.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIVEMTREAT
                     PKEIDKCKLLYCGHGFYDEPNIQNNINKNLRD"
     gene            10818..11651
                     /gene="terS"
                     /locus_tag="ACHBFW_001166"
     CDS             10818..11651
                     /gene="terS"
                     /locus_tag="ACHBFW_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFM9317486.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11644..13059
                     /locus_tag="ACHBFW_001167"
     CDS             11644..13059
                     /locus_tag="ACHBFW_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFM9317487.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13071..14600
                     /locus_tag="ACHBFW_001168"
     CDS             13071..14600
                     /locus_tag="ACHBFW_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM9317488.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEYMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKKLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14518..15570
                     /locus_tag="ACHBFW_001169"
     CDS             14518..15570
                     /locus_tag="ACHBFW_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFM9317489.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15572..15889
                     /locus_tag="ACHBFW_001170"
     CDS             15572..15889
                     /locus_tag="ACHBFW_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317490.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16016..16639
                     /locus_tag="ACHBFW_001171"
     CDS             16016..16639
                     /locus_tag="ACHBFW_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFM9317491.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16654..17544
                     /locus_tag="ACHBFW_001172"
     CDS             16654..17544
                     /locus_tag="ACHBFW_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFM9317492.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17567..17782
                     /locus_tag="ACHBFW_001173"
     CDS             17567..17782
                     /locus_tag="ACHBFW_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317493.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            17794..18135
                     /locus_tag="ACHBFW_001174"
     CDS             17794..18135
                     /locus_tag="ACHBFW_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317494.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18135..18506
                     /locus_tag="ACHBFW_001175"
     CDS             18135..18506
                     /locus_tag="ACHBFW_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317495.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18499..18894
                     /locus_tag="ACHBFW_001176"
     CDS             18499..18894
                     /locus_tag="ACHBFW_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFM9317496.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            18896..19273
                     /locus_tag="ACHBFW_001177"
     CDS             18896..19273
                     /locus_tag="ACHBFW_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFM9317497.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19274..19882
                     /locus_tag="ACHBFW_001178"
     CDS             19274..19882
                     /locus_tag="ACHBFW_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM9317498.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            19882..20223
                     /locus_tag="ACHBFW_001179"
     CDS             19882..20223
                     /locus_tag="ACHBFW_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317499.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGPVVEEAAEVMEG
                     MQNAVDGKSVSEALSRAYSFIADILFEGQFTGEDYRQGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20465..22254
                     /locus_tag="ACHBFW_001180"
                     /pseudo
     CDS             20465..22254
                     /locus_tag="ACHBFW_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
BASE COUNT         8057 a         3253 c         4613 g         6331 t
ORIGIN      
        1 aatgttcaag attatgagga agccctaaat attcaataat ttggaactga ctcatatcag
       61 ataaatcttg ttcatctatt gttctagtta acatttcaat tgcaaacata ttagcttcag
      121 cttctatacc ataaatcata caactaaggt tgtttgctct aaaagctgct gtgcaaatac
      181 ctttatggag tctaacgtgg gctatttcat gccataatac aaatcttttc atagaaggtg
      241 ataggttttg attaattaat atcacataac atcttttatt gtgtatcgtt tgtcctaatg
      301 ttttattccc taaatcagtt tcaattaaat ctatatcaga atatttgatt attttatatg
      361 gattaatcgc atcagatcca aactctacaa ataatttttc tatttcttct aatatccagt
      421 atttgtttaa atgcatacca aagcccacct attgacgata ttttttagga gtgaattttt
      481 tctttgcttt aactttggtt aaggcaagtg caagttgaat ggcatcgtat aatttttcct
      541 tatcttcatc gtctaaaggt tcaccataat aatttgtttc agcatctgaa ttcaagcctt
      601 caagtaattt ttcagcttgt aaagcaatat cttgtttatc tttttctgtt aaatcataat
      661 aatggcgttt atcagtacgt cctaaaagat aatcagttga tacattaaag taatctgcaa
      721 ctttttgtat tttatctcca gatggagtac ttttattcca tctagacatg cttccatttc
      781 cgaaatttaa ctttctttca agttctgcca acgtcattga acgttctttt aataagattt
      841 ttatacggtc taatagcata ggatatgcat tcctttcaaa ataccgacaa aaaaagtatt
      901 tttctgcaaa ttatctattt acaaatagag aatttgcagt tatactagtt ttgtaagcaa
      961 gatatttacg gcatacaaag actaaaagca ataaactaat agtctcccca gacatcagta
     1021 agctcgcttt ttgtgcgctg tattttgtat gcttacataa tagcatattt gcagtttttg
     1081 tcaaacttat tagacaaaaa tattagaaaa tattcgataa gggggtatga agcatgactt
     1141 taaaacagca agtattaatt gctttagtaa agaaaggttg gagtcaaaga gagttagctc
     1201 gacgaatggg tatatcaatt acttatttac gagatatctt catcgaaaaa agaaaaccta
     1261 aggaacgttt aaaacaaatc gaagaacttt tggatatcaa gttagaagta gattctaatg
     1321 atcagccatc agaacaggag gcgtaaaaat ggtacaacgt ttgagcaaag cggagtttaa
     1381 agagaaatat aagtattctg aaagtactta tcaaagaaga atgaaggaat ttaaaaaatc
     1441 acgttttagt gaggggtata acgcagttac ttccaaagaa ataacaattg atgtcgattt
     1501 atatgaacag tttagaacat ggcgttctaa aaatcgattt agagttagag ctgtttgtta
     1561 atagctaaaa aatctaaatt caccacttct gaaaagagga gttgctgctc tcctttcaag
     1621 tttgccaaag tccacgtttt ttcattttgg caactcctct tcttggaggt ggtgacggcg
     1681 agagacgaag gagggatgac atgaaggaag aatcaaaaat agggattttg aatgagaaca
     1741 attacacaag attaattatt gaaacagaag aagggaaaaa agtagcagaa gttacattga
     1801 ccgatgcaac tcctgctgac ggttatcgta ttcgattaac tcctaaatat gattaacctt
     1861 ttggtgggtg tggatcgttt ccgtgactat cacggctttg aatttttcca tctctgccat
     1921 gaataaatag ctcagatcct tgatttttag aaatctctct agcgattttt actgcttctg
     1981 attttgtatt agtatgtgca gtcgctttag aatttccagc acctttgaca ttccaaccac
     2041 cattggaatt aggtacaaca tgttgatttt ttcccatagt tttcacctcc atttctatag
     2101 aaatatttta tcaaatagaa atggagaaat tcaataaacg aaaggagaag tgatatggca
     2161 tataccttgc aacaagaaca tcaaattctc ggtttgatta aacaacgcag gaaacaatta
     2221 caagatgacc gtgcagcgct tagaaaagcc gatgagctat cagataggca agctgaacta
     2281 attgcttctg aacttgagga tttgagaatg ctagaaataa aaaataggga gattagatta
     2341 tgaagaagac agacacactt tttataggat tcattttggg gttattagtg attgtagcgc
     2401 accaaagtat tatcggggga agcttgttcg cagcattgat ggttttaatc aatctgcttg
     2461 attcaaaaga aaggagcaac tatggcacga gaagaagcgc taaaaatcgg taaagtgatt
     2521 gctgataatc ggtggggaaa tagccgccct attattttaa gcaagcaaca tatcgaaaag
     2581 caaaaagcat ggcaacaaat aaaaaagtga ctctgccagc cagcaaagag tcacatacaa
     2641 atttataaaa ctaggagaat tatatcacat gaataagaaa attgaaaatc ttatcgaaga
     2701 attgaaacgt gaatgccaaa agcaaggtgt ttctattatt tgtacagccc aaaaagaagg
     2761 ggaacttaaa agtctcgttt atggtgagac aacagaaatt ttactttgtc ttgctatgca
     2821 agaagaacat ttagatgaaa atctcccatt gtcagcacat attatgcgta gaattgctgt
     2881 ggatgcttac gaacaagcga aaaatgaaga agaaaatcaa ccttcaaatc atacttttgt
     2941 tattaataac aaggaagatt tagctgatgt gatgactcgt attctcaagg gggaatttca
     3001 atgatgaatg aaaaaacaat tgatctactc attattttat ttttcacgtt agtgattgta
     3061 gcgaaaagtt atgtaatctt tggcttgtta ttcgcaatta ccatgcttgt gctttgcgtg
     3121 ttagcaagca gaaaagaggt taaacatgaa cgagttagca aatcttgaca actatttaac
     3181 tgatcctgat tatgctaaac caccatatga agcaccaatt gatgaggagg atgaagatga
     3241 gtaaatctac cttagaaatg agccatcaag aatggcttga agaccgtaag aaaggcatcg
     3301 gaggttcgga tgttggaaca attttaggat tgaataaatg gaaatctcct tatcaactat
     3361 ggctagaaaa aacaggacaa gtagtacttg aagaatcagg aagtgagccc gcttattggg
     3421 gtaatgtttt agaagaagtg gttgctaaag agtttcagga acgtacaggc aaaaaagtac
     3481 gcagaagaaa ccaagtattt gaacatccat tacatccatt tttaagagca aatattgatc
     3541 gggaagtagt gggagaaaat gccattctgg aatgcaaaac agctaatcaa tttctcggta
     3601 aagaatggga aggcgaagag gtaccgctta gctatctctg tcaagttcaa cattacatga
     3661 acgttctaaa caaagactat tgttacatcg ctgtcttaat cggtggtcaa aaatttatct
     3721 ggaagcggat tgaacgagat caagagctga tcgatacaat cactgaacaa ttagtagagt
     3781 tttgggaaac aaacgttctt ggaggtatcg agccaattat tgatggtagt caagcgacag
     3841 ctgatttctt gaaagagaag tatgcagatg ttgaagacgt tcaaacgacg ttaccaattc
     3901 atttcgatga actagttgaa cagaaaaacg aactcaaacg gactaagaaa gaaattgaat
     3961 cagctatccg tcaagtagac aacgagatta tcagcgagct aggtaaacgt gaagccagta
     4021 tcggtatcac tcaaaaaaac atcatcagtt ggaaatttgt ccgtacgaga cgtatgaact
     4081 cgaagaaact agcagagaaa tatccagatg tcgcaaatga tgaagagatt tataacgtta
     4141 ctgaatcaag aaggctaacc gaaagggaga tcaaataata tggcaacaaa tgaatcgtta
     4201 aaaaatcaat tggcagcaaa gccacagaaa caagttgcac caggtcaatt aggacttaaa
     4261 gctttgatga atacaccaac aatgagaaag aagttcgaag aggtgcttca tgacaatgct
     4321 aatgctttta tgtcgaatgt tatgactctt gtatctaatg acagttatct tgcagatagt
     4381 gaaccgatgt ctatcatgag tggtgcgtta actgctgcaa cattaaatct tgggctagat
     4441 aagaatttag gttatgcata tttagttcca ttcaatagta aaaacaagca aacaggaaaa
     4501 tgggaaaaga aagctcaatt catgcttggc tataaaggat atatccaatt agcccaacga
     4561 tcaggtaaat acaaagcatt aaatgtgatc gaagtttacg aaggagaact aaaaagctgg
     4621 aaccgactga cagaagagtt tgagtttgat ccaaatggta gaacgtctga tgaagtcatt
     4681 ggatatgttg gctatttcga gttactgaat ggattcaaaa aaactgtcta ttggaccaaa
     4741 caagaaatcg aagctcatcg gattgctaac aataaagatc gcgataagac aaagttaagt
     4801 ggtgtgtggg catctgatta caatgcaatg gcacgaaaaa ctgttttgag aaatcttctt
     4861 tctaaatggg gaatcttgtc catcgaaatg caagaagcca ccacatcgga tgagagagtc
     4921 caaagggttc aagaagacgg cagcattatt gctgaaacag aagttgaaga agatattcct
     4981 gaaagaaaag aagcagaggt tatatctgaa gaaaatgaag atgtacaaac tggattattt
     5041 gatgcatcta atccgccgtt aaacaaataa tgagggagct ttctcccttg cttttctaga
     5101 ggagaaatac gaatgaacac aggatatgtc aaattacatc gaaaagtgat gaattcattc
     5161 gtttggacca atccttatat gtataaatta tggaacttgt gtttgatgaa agcgagtcat
     5221 gagaatcgca agatactttt taatggaaaa gagatacaag tgaacagcgg agaattcgtc
     5281 acagggcgcg atgctatcac atctgagatg aacgaaggcg tgaaacgtga acatcaagtg
     5341 aacagcggtt ctgtatggag atggctaaaa caatttgaaa aaaacggaat gttgaacatc
     5401 aaatcaacta cgaaatacag cgttatatca ataaaaaact ggtctttgta ccaaagcagt
     5461 gaacaacaaa tgaacatcaa acgcacaaca agtgaacaac aagtgcacac aaacaagaat
     5521 gaaaagaatt ataagaatga aaagaataat aaaacatcat cgtcgcaacc acgcaaaaag
     5581 cgtgtttacg acaccgactc agtttactac attctcgcgg aggagttatt caaacagatt
     5641 tgtcagaatc aggaaatcaa aaagccgaat ctgcaaagtt gggcagataa cattcggaaa
     5701 atgatcgaga tcgacaagcg gaccgaaaac caggtacggg gaatgattga atggagtcag
     5761 cacaatgtgt tttgggcatc gaatatttta tctgctaaaa aattgcgaga aaaatacgac
     5821 acaatggcag cgcaagccaa tcgtgattat aaaacaaaac aaactaaaac gcttgaatac
     5881 gagaaattta gcacagatga gttgcctatt tgagaggagg cgtaggcatg gaaactgttg
     5941 gggaaatcat ggaaaagctg atacagaaag tgcttgtcca acgtggcgaa tgtcctgaat
     6001 gcggacagcc tttgtatgga tggcgtacga agaaccctga tggctcagaa cgttgtaagc
     6061 caacctgtat gcaatgtggg tataaagcat tgcgagttca ggaagactta caaacagaac
     6121 gaatttacaa cgagagcctg aaagcaagag caatcaattt tttcaaaggt ggttctgttg
     6181 tgcctaatca agcgttgttt gattgcacat tgcagaatta tcaaattgtc gatcaagaaa
     6241 caagacaagc ggttgaagta accaaacgct ttgttaattc agtcttgtta ggaaatccaa
     6301 gtcaccttgt tttaactgga aaacaaggaa caggtaaaag tcacctagcg atggcagcgg
     6361 cttgggaagt cttgaagcga tcaaactacg ataaaaaaat cttatttatc gggttacagg
     6421 aaatgctgga tcaaatcaaa ttttcgtaca acaatcctga actcagaaaa acgattgagg
     6481 gatcgttgat tgcagatatc aaaacagcgg atttagtcat cattgatgat atcggttcag
     6541 aactaggaaa agatgcatca gatagtcgag cgtttggcat aaacacgcta aattcgttct
     6601 tggatgcacg acagaaccta gcaacgatta tcacaacgaa cttgcttggt gaagaactga
     6661 aaaaagctta cggtacgaga acaatatcaa gaatgtttgt caactctgat ggatttacga
     6721 tggtattttc tcaaacagca gacaagcgca taaaaccagt caaaggagcg atcgcatgaa
     6781 tttgactaaa caagaaaaaa gaatctgtcg tcagataatg ttgcaaatta taggtgatcg
     6841 tgacatggac gaattatctc aacaagagag tcgaatggtt cggaagataa gtaagctaat
     6901 tatttatcgg gatacagaaa gatttctcac tgttttaggt aacgtctacg agaatccaga
     6961 gttattggag gtagagtaat tgccaaaaat attagatgca tgttgcggta gtagattgtt
     7021 ctggtttgat aagaacaatt ctgacgtaac atttatggat tgtcgtcagc agtatgaaga
     7081 attatcaaca ggtcatgtga tcaatgttga tccagatgtg gtcgcagact ttcgcgatat
     7141 gccttttgaa gatgatgtgt tcgatatggt tgtatttgac ccgccacact taagacatgt
     7201 cggcgaaaac tcatggcttg ccaaaaaata tggaaaatta gacgaacttt ggccagaaga
     7261 catcaggcaa ggttttgcag agtgtatgcg agttttacga ccttcaggaa cattaatttt
     7321 taagtggaac gaggaacaga taccactttc tgatgtgtta gaggctattg gagaacagcc
     7381 attgtttggt aataaacgaa gcaaaacaca ttggcttgtg tttatgaaaa aattcagcta
     7441 acggaggaaa caaaggagga aaagtaatga agattgtatt aaataaatgt tttggtggct
     7501 ttgggttgag ccatgaagca aaaatggaaa tttttaaaag aaaaaatatt gaagtattcc
     7561 catacatcaa taactttagc tatgactcag atgatgaata cacgagatac acaggccaaa
     7621 agctaggttc gatggatttt atctactatt tcaaaaaaga cccgaaaatc gacaaagtta
     7681 caggaattta tagcgaaatt gaccgactgt acggaattgc tgatgattcc agtttttcaa
     7741 gtgattcaaa cagaggtgat aaggacctcg ttgctgttgt cgaaaaacta ggaagtgagg
     7801 ccagtggacc atatgcaagt cttaaagtcg ttgatatacc cgatggggct gagtgggaaa
     7861 taagtgatta tgacggcgtt gaaactgcac attatggttt tcaaacaggt agtatttagt
     7921 ccactaatga caactaaaga ggaggcagaa ggatgagtga agatgaatac tttgaacagt
     7981 agtctattaa cgacagagat ttttaactgg aaaggggtgt agtcatggac gcatcggaag
     8041 tagtaaataa aattaatagt ctcaaagagg ttttcggaga cgtcgaagta gtgataaacg
     8101 cacaaggcta tgacaacgga ttttttaagc agattaccga cattagcatt cagcaaggaa
     8161 tggaagacga agatggaaac tttatcgatg aagtagccat tttagtgact tgcgagtaag
     8221 taattgacag ctaaagagga ggcagaagga tgaaaaaatt gcaccaactc atatctgaga
     8281 aagaaagcga actccaaaat ttagaagata gtttgggatt ggggtttcca attgttgagc
     8341 aagctaaaat gactcaaatc agtcatctac gacttgaatt ggaagattta agacaaatag
     8401 aaaaatcaat tcaactcaac gacaaccaga aaatcgtgtt ggagtggttg aaaagcgaga
     8461 caattttaac tagagaagca ccaatattat ctgttaatgc tttttctgat aaaaatttac
     8521 taggaaaatt acctgataaa gtacgcaaag cttataaact attggcttgt aaacaagaat
     8581 atgaagtcct atcagcattc gctcaatggg gattagaaca ggaggaagcg gaatgaacaa
     8641 cagacatcgc agaataacaa aactaagaaa acaggaactg aatgtactaa agacaaagtt
     8701 tgaaaaagaa tatggaattt cagcagaaga aacatataaa gtggcaagtc agtttgttgc
     8761 tgatgcgagt aatgctattc gtaagtttgg gatttcgata ttaaatgatg atcgtaaatg
     8821 ggaggaaatg agatgaaaat aaaagacgga ttttacgcta gtagtcatgg tatcggcggt
     8881 ttaatgctag acatgccgat aaagaaccct aaaacacgtc agaaatcaaa attcaaaatc
     8941 ggtgacatgg tccgatgtaa agcagaagag ttcatctatc cgtttcgtgg atatgtagag
     9001 catgtctata atcattcagc aatcattcac attgaaaaca cgatggaatg cgataagtgg
     9061 ttagcgaaaa gcaaagataa tttagcagtg gctcgtttgg tggatatgga actaatcaat
     9121 gacaaataaa aaagccggat cgctccgact gattcaataa atccaacaca tttattatat
     9181 cacataaagg agcggtttga cttgatgcaa ttgttacgag aggtagattt caaacagaca
     9241 agatgtaatg cgagagatgt gctgaagaac tttcggcgtt tggagcggat ggcaggtcgc
     9301 tctttgatag atattaagtc gccgattatt acggatatgc cgaaggcacc gaagcacggc
     9361 aataaggcag aagacgcgat cattcagatg atggatatag aagcggagag agacgcaata
     9421 ctagcggctt tgatggctct tagtctgatt agccgtcaga tactctacta cagcttctgt
     9481 gacgtaaaca agcactctaa ttatgaaata gggcaattga tacgaggata cggagagaag
     9541 aatgtagaga agttgaaatc catcgcatta atcgaatttg cagaagcata caaaaaaggc
     9601 gtgttagttc agtatcgttg attttgtagg gtttttgtag ggatagtgta gggtttttga
     9661 gcggtttaac gtgatattat ggtagtgtcg aaagattagt gataggtctg agacaaaata
     9721 aaatgtaagg gaggaaatct ccctcatcgt tttaacttaa gcttcgatag acagcaacgg
     9781 aaatattaag aataaggatg tgaatttcaa ctccttctaa attgttctta ttatctatca
     9841 tccgttgttg tctattaatt tatgcatcgg aggaaaaacg aatggataaa gaaatcaaag
     9901 taactgtcaa attagatttg actgaactaa aagaactgct caacaaggct agtgaccaag
     9961 tcgaacagtt acaagaaact ttagatgaaa ttgctaattt taaaatccaa gtttcttagc
    10021 agtatatttt ttagcagctg cactcataaa atcataccaa gtatcagaaa aatataactt
    10081 aactaaataa ttaaaaaagg tgggtaaata gaaatgacgg cgttaataat ttcaattttt
    10141 gcgctctgtc ttaatgtcta tatgattgga tttaaaaatg gacaaaataa aaaatagtag
    10201 cagccgagaa taattttata gtgtcactgt ggcggaaata gaagacgcaa aggtggaggt
    10261 aggaagcgta tggcgcacta tcgggacctt gtacaaaact agaaaggact agtacgtgtg
    10321 tggtgcgagc cctagggagg aacaggataa ccgcctgtgt gtaggttgct attacatatt
    10381 agatcactct ttgagtggtc tttttattta cataaaggag gctacataat gagaaactac
    10441 tggtatatat cgctaactaa tgaatatcct cgaaccattg atgattgttc agtgcgtgtt
    10501 gtgcgttctg tccaaatcaa aaagaagtac tccattgttg aaatgaccag agaagctacg
    10561 ccaaaagaga ttgataaatg caaacttctt tattgcggtc atggtttcta tgatgaacca
    10621 aacattcaaa ataatattaa caagaatttg agggattaga atgcaagaaa tggcttaaaa
    10681 atctccaatc aggacaaatc aagttagaca aagtttccga tttaaagata ttgattgaag
    10741 ctgatttaat gctaaaagat attgaaaatt agaaaacaaa actcaaccta agaagattgc
    10801 gaggtggtgt gtattgaatg gcaagacaac gtgatccaag acgtgatgaa gccaaaagaa
    10861 tttggttaga atccaacgga gaaaagcagt taaaggaaat tgcatctgaa ttaaatgttt
    10921 cagattctca ggttagaaaa tggaaatcgc aagacaaatg gagcgctgaa ttgaaaagta
    10981 acgttaccaa tggcaaaagt aacgttacta atcaaggtgg cgctcctatt ggtaatcaaa
    11041 atgctaaagg taacaaagga aacagcagag catcaccgcc aaagagaaat aagaatgctc
    11101 ttaaaactgg tgagtatgaa acaatatttt ttgacacgtt aaccgataat gagaaggaca
    11161 tctattctag tttgaatgat gatccttctt ttgctttgtc cgaagaaata cgacttctta
    11221 aaataagaca acttcgaatg atgaaaagaa tccaacaagc tgaagctgga ctaaatgatg
    11281 aagaagtcga acgattgcag cagctaagaa agattaaaac tccgattgaa aaaaatggta
    11341 aaaagctaga aatcaagcgt gaggttatgc aagatgtgca gattagcaga aaaaaacatc
    11401 gcaaaattga tgatattctt tcaattgaag attcattgac tcggattagc aaccagttag
    11461 ctaaagccat caagcaaatg aatgaacttt atatgaatga atacagaact gatttaatta
    11521 aagctcagac tgataagatc caagctgaga caaatgaaat tggcggaaat aattcaggtg
    11581 aagaaataga agaatggaaa caggcagttt taaatgccgc aaacaaacgg gcggtgaaag
    11641 aaaatgaata atgaatttat tccttttgct gatattggtt ctgccattga ttattactat
    11701 gataaaccag tagctttttg ccaagatatt ttgcatttga atcctgatga atggcaagaa
    11761 aatgttttaa atgatttagc tgaattttca aaggtttctg ttcgttctgg tcaaggagtt
    11821 ggaaaaacag cattagaagc aggagcaata ctttggttct taacgtgtcg accctacgct
    11881 aaagtaatag caacagctcc gacaatgaag caactttacg atgtactttg ggcagaggta
    11941 gctaaatggt taaatgatag cttgatcaaa aacttactga agtggacaaa gaccaaaatt
    12001 tatatggttg gtgattcaga gcgttggttt gctacggcta gaacagcgac taaaccagaa
    12061 aatatgcaag gttttcacga ggaccatatg ttgattgtgg tagatgaagc ttctggtgtg
    12121 tctgatccaa ttatggaagc tattcttggt acgctatcag gttttgataa taagctgttg
    12181 atgtgtggaa accctaataa tattgaaggt gtcttttacg attcccacaa ttcagaccgt
    12241 gataaataca gagttcataa agtatcaagc tatgatagta aacgtacaaa caaagacaat
    12301 atagaaatga ttcttaaaaa atatggaaaa gaaagtgatg ttgctcgtgt ccgtattttt
    12361 ggagaatttc ccaaaggtgc gttggattca tttatcagtc ttgaaacggt tgaattggct
    12421 acagaaaaac aaattagtga ttctttagtc aataaaacaa cggttgctca tattggtgtt
    12481 gacgtagctc gatatggtga tgattctacg attctctttc ctagaattgc taccagggca
    12541 ttggagtatg agaagtattc aaaacgtagc actatggaaa caacaggata tgtcatcaac
    12601 atggccaaga atctaatgag tcaatatccg agtattgata aagtgatgat taaagtcgat
    12661 gacactggtg tcggaggtgg tgtaaccgac cgcctagaag aacttataga agacaagcat
    12721 tatccttttg aggtgtttgg agtgaataac ggttcaacat cagaagacga tttttacgat
    12781 aatttaggta ctcaactatg gggaaacatc aaggaaatgt tagaagaaaa tatgacagca
    12841 aatcttaacg gagaacagcc tgttattgaa ttgccttctg atagttcgtt aatcaaagaa
    12901 ttaagtactc gcaaattcaa aatgacaagt agaagtcgta tacgtttaga aagtaaagat
    12961 gatatgaaaa agcgaaatat tggtagtccc gatattgctg acgcactggc tttagcgttt
    13021 tatgagccac caagtcacta tcaatttatt caattttagg aggtgagttt ttgattagta
    13081 cagtattatc tttagagaga tataagaagc tacgtgttaa atatgcaaca caaattgaag
    13141 atggaatgtt tgatccgaat ggttttatag aatatatgaa accatttttt gctgatcgcg
    13201 aaagaaaata tctagcttat actagcgaaa agaatgaaat agacaataga ccaaagccta
    13261 acactgatat tgtaaaagtt aataataaac ttcatgctgg tatgtattca attgtcgtag
    13321 accaagcagt caatcatttt actggtatac ctatcaaatg ggattacgat gtatcggaac
    13381 aaaaaagaac actcattcaa agattaaaag ataaattctt aaaaaatgat atagaacttc
    13441 caacagttcc agaagctttt aacaaattaa caagcaatct tgattccatg agattcgcaa
    13501 tgcttgattc tgaaactgca acttttcaag gagcttgtgg agtagctttc cgattgttag
    13561 agcctgtgga agaagatgat ggttggaagt taagagcaag taatattgaa ccttggagag
    13621 cagaaagata tggaaatgcc ggaatctata tcaaagaaaa gtatgactct taccagaaaa
    13681 aattttttca agaaatgaaa gttatgacaa gaaataaaat tctgacatat gcttgctatg
    13741 gtgatttgaa ttttgttaca agcggaacgt tcaaaaaaat tgatgagacc gataaccctt
    13801 tggggacgat taccttggca gaatttaaaa ataatacgaa tcgttattgt gattttgaag
    13861 tagctgagga aattggagat gcaattgatc gtgctttgtc tgatcaacaa aacgaaatcg
    13921 aacaatttaa gcttgcgtac atgcttgtta ctggcacaac aatgagcaaa ggcactgcta
    13981 aagaaatgat gaatcagcta gggattatca acttaaaaga ccctactgca aaagctgaat
    14041 atgtcacaaa aaaattagca aaagatttca atgaatatca tatggatcta ttgaaaaaac
    14101 agttttacac catttgtaaa gcaatcgatt ttaacgatga ggtgtttaaa tcaaacagtt
    14161 ctggagaagc tcgcaagtgg caaatcatta gcctagaagc gaaaacaaat actaaagagc
    14221 aatattttag agaaggattg aaagaatgcg cagaaacaat tgctgctttt cttaagtttc
    14281 atgacaaagt agaaattgag cctgaaaaaa ttatttttac tttctctaga tctttgccaa
    14341 ctgatttaag ttatttagct gaagctttac ctaaacttgc accatacgta tccaaacgaa
    14401 ctattcagag ccaaattcca tttgtaacag atgttgatta tgaaaatgaa atgatggaat
    14461 tggaaagtgg aagtgcttat ccagatagtg aatacaattt tggcggaggt ggcaatagtg
    14521 acgatagaaa cgaagtattg gaccaaacgt cgagaactgg aggatcaagc aaggctcaaa
    14581 caagaaaatc agacacttaa aaaattaact agtgtatttc ctgaagcact gaaagagata
    14641 caagcaaaac tattatcaca agctgactta cacaatatca cttatccaga aatgatggag
    14701 ttttacagta caagtaatca gaaaaaatat cgtgaatatg tggaaaaaaa ttataagtca
    14761 ttaaaaatgt atgatgcaaa atacaaagag tttatcgatg aattttttcc accatttgac
    14821 tatgcaaaag tcaatcgctt attacaaata cgatcagatg tatttaaaat tcttgcagaa
    14881 tatgcgatgg atgcagatgt gaatcaatat ttttctgatc gcttagagga aattcttcaa
    14941 agaacatatt cttctaatgc taatgttttt gttcaacttt taaacgttga tataccaaat
    15001 tatttaccag aaaatgaaat taaacattat ttgaactatc catggtcagg aaagacattt
    15061 tcaagaagac tttggggaaa tatttcatcg cttgaacaga aactctctaa tgctattgta
    15121 aaaagtgttg ctagtggaga aggcgttata cacgcattaa acaccatgag attagactca
    15181 gaaatttgtg acatgtttaa gttagaagaa tcaaagtata acaaagcgat agaaaatctc
    15241 gttcgaacgg agtatgcaaa atttgcacaa gatggtattg aaaaatcata tttagaaaca
    15301 ggtattgagg aatacaacgt attgactgca aaagatgaga gagtttgccg gatttgtgga
    15361 ggaaaggcaa gtaagaatcc ctataaactg aaagatgctg tcataggtga aaatcgagca
    15421 cctttccata gtcgttgtag atgtacggat gttcctaatt taccaaaatt aggaaaggat
    15481 attgatgaag aatatgaccg tttatttggc gatttattag atgagtttgc acatgattct
    15541 tttggaatta atttgaaacg gaggaagtag aatgaaagat ttttttgaag cagtactaac
    15601 aattaatgta aatgctgata ttgcagaagc ctacaaaaca gctattgagt ctgagaacca
    15661 tcctaatggc ttgagagacc attggaatgg caattatgcc tacgtggtta ttggcgatca
    15721 aactgttaat tatcaagata atactccagt tgataagaat accgttaatt taacgattca
    15781 attattatct cattcattac caaatttaaa agaaacagtt gattggtatg aaaagatggg
    15841 atgtattgtt gttagaactg actacaaaga aggaaagtct agtaattagg cttttttatt
    15901 ttgtccgaaa tgacgttaaa ctagcgcaat actgggcttg gttgaatggt ggggcgcaac
    15961 tattaaaact caaagcaatg cggggcgtga aaacgaatcg tggggcgaaa ggagaatgag
    16021 catgaaacac aaatcattaa tgccaatgaa tttgcaatac tttgccgaag gtgacgatca
    16081 aaagttttct tttgatgact tcaaatcttt tgtggaatcc aatgaagaag ctcaaaaatt
    16141 tattcagtca caatctcaaa gtgttgccga caaacaattg gaagcttgga aacaaaataa
    16201 cttagataaa attaaacagg ataccatcaa ggaatatgag gaatctaaga aaaacaagtc
    16261 acctgagcaa attcaactgg aaaaactaca agctgaattt gaagcagaaa aagcgttgcg
    16321 tgtgacaagt gataataaag catttgttgc agaacaaatt gctggattag aactagatgg
    16381 agagttaaaa gagtctattt ctcaatttat gctaaataat cttgttagtt cggatacaga
    16441 tttcactaag aatgctgttg aaggttttac aagtgttttg aatgcaatta atgagaaaca
    16501 tgcagatgca ttaaaagaac tacaaatgaa gtctgcattt ggtggaactc aacaatcgaa
    16561 taaccaggtt cagcagaaca atgaaacatt aacaaatcca gaagaacaat taggacaaat
    16621 tcttcaacaa tttaactagg agagtgaaaa attatgaaaa aaacatcttt aaataattta
    16681 gagtatttgg atatttcacc agcgattaat gctatgcaag taccaaatac acctttttta
    16741 agctatttac ttggtgctgg aaaaacagag ccagcaaact cgacagaaat taaatggcgt
    16801 gaatatgata tcaacaacga tgattcttct gaaaaacttg agggcggaga atatccagat
    16861 gctgaatcag gtcgaacttg gtttaacaac tatactgaaa tttttagaaa atcaacctct
    16921 gtatctggta cattagatgc tattaatgtg aatggagtcg gaaatgaatt aactaatcaa
    16981 gtagcactac gtggtatgga aatgaaaatt gatttgaacc gaaaattgat tactggtgta
    17041 aaagctgatg aaaatggttc taaaggtcgt cgaatgaatg ggattttgaa cttgatcaat
    17101 tcagcaaata aggcagaaac agccactgcg ggtgcagtaa caagaaaaga tatcgatact
    17161 ttatttaaat tgatgtatga aaaaggttat atgggagaaa aactatgctt gatttctcca
    17221 gatatgcagg agttaatgac tgatgagtta gatggaaaat caacaaaaat tgttcagttc
    17281 ggggaaagag taacttttgg attgcaaatt ggaaatatcg tgtctaatta cggtacaggt
    17341 attgctctac tagaaccatc attgccaaaa ggaacaattg ccgcaatcga tactaattat
    17401 gtgaaactac gtccattacg tgaatggaga gcagaagaac ttgcaaaaac aacggattct
    17461 agacgtatcg gtcttgtagg tgaatactct cttgaataca atgcttcaaa ctctggggca
    17521 attttaaatt taaagtctga ataaaaggga gttagtactc ccttttttga taggaggaat
    17581 tacagtggta aaaaaagatg aaactaaaaa agatgaagtc gtgaaatata gagtaggtaa
    17641 aactaaaaat tttgttgggt ttgttcatcc taaaactcgt agatttatca cagcagattc
    17701 aaataacgaa tttatcattt ctatagatga taaagaagca attgcaattt tggaagatgc
    17761 aattgatgtt aatgaaattt aggaagtgat ctgatggatg aatcgctaaa aacggaaatc
    17821 attgagtcta caaaagaaga ttttccagat ttgagtgaag aacgcataac taatttatta
    17881 gaaataattt tgctagaaat tgaatcatac aacacttgta aaaatgatat ttcatgggaa
    17941 aagttaaaaa gcgtgattag tgaagtgttg tataaaataa taaaaaatga atcagaaaaa
    18001 acagtatctt cagttagacg tggcgataca acgattagtt atgcttcaac aacaaatgat
    18061 gttagtgagt tacttctagg ctatggtgat ttgatacgaa gagttattgg ttgtggagga
    18121 ttggagtttt tttaatgaat gaagcagata ttttagaaac tacttatgaa gatagttgta
    18181 ttattgaaag actgacggac attgaagatt ctaatacaaa tattactatt caagattaca
    18241 aaaaagtata tgataatcct atttcttgcg ctctttcaca aggtcaaatc gatggactag
    18301 cagtcataga agatggagag atggtaaatg tttcaactga cacatataaa ttatttgttc
    18361 atcctaagat taaactaaaa aaaggagatc gaataacaat aactcaaaag gccagtggct
    18421 taattttttc tctatttgct actaagcctt tttactatcc tagtcattgt gaagtgaatt
    18481 tgataggaag tgagaaaaat gggtaatctc aaatatgaat caaatgcaga aaagatcatt
    18541 gagaatttta aaaatatgac tgtaattgct caaaaagaag gaatatcctt cgttaacgat
    18601 tcaatgaata aagtcgttag tttagctaaa ccattaactc ctgtaaaatc aggtaattta
    18661 aggcgaggat atcgtgtagt aaaagctaga aaactatcaa gcggtcgaat tgttggagca
    18721 gttataaaca atgaacatta ttttaaatat gtagaagaag gtcataggac taaaaatggt
    18781 ggctttgtca aagggaaatt catgttaact cgtgcaacga atcttgcaaa tatgtcttat
    18841 attcctcgaa gatttaaaca aatggcaata aaaatcgtta agaaaggaaa gtaacatgaa
    18901 agatgaaatc attgctgcaa tcagtagcaa attaaaagaa atctatcctg atgggacaat
    18961 atatcttgat tcagttatgc agtcaactaa agatttttat ttcgttttat cagtaatgga
    19021 atctggaact gaaaatgtag gaattgatat tcaaaatgtt tctttcttaa ttgatattgc
    19081 attgattgat aataaaccta atagaaattt aattaatgaa ttagtctcac gttgtgggac
    19141 tttttttaat acgattacaa tcgatgagca aatattattt ccaaaagcct atttacctga
    19201 tgaagcagat ggtgttcaac atattcgttt tacattagaa tttccacaat atattgaatg
    19261 gagtgaaaga taaatgggag aaaaaagaag taaaaccgga attatttctg tcgaaaaacc
    19321 tacttggttt cctttggaag atgagacagg gaattttccg gtatatggta ctgcgttgcc
    19381 aatgggaaca gcagtaagta tcaaaccaac agctaattat gaaacaacac aagattatgg
    19441 ggattcagtg gttcaagatc aatttacagc ttttggtggt gcagaagttg aactggaagc
    19501 aaatggatat actcataaag ttttatctac aattactgga gggaaaattg ttaaaggtgg
    19561 tgccttgcgt tctggagaag atattgcgca agatggtgcg tttgcatata gacgtaaaaa
    19621 atcgaatggg aaataccgtt atacggtctt ttataaagga cagtttgctc tagattcaga
    19681 tgaaacatca acgattgaag gaagtaaagt aagttttact catccaactt ggaagggttc
    19741 atttgtagat gtaccaggac ttggatatat gtattcagtt gatgaagatg atgaaggcgt
    19801 ggataaagca atgatcggaa attggtttac aaaagtagca attccaattg aagaagcaga
    19861 actttcagga ggtacagaat aatgtcaaaa tatcaaacaa cgattaaatt aacgaaaaaa
    19921 gatgaagaag gtaaatatga gcaagtacaa tttaaatctg ctgaatttct accaggccca
    19981 gtcgtagaag aagcagcaga agtaatggag ggaatgcaaa atgctgtaga tggaaaatca
    20041 gtatcagaag cattaagtcg agcttattca tttattgcag atattttgtt tgaaggacag
    20101 tttactggtg aagattatcg tcaagggatc gatgctcgag aaattgcttc gttgacaggt
    20161 aagttattaa aatctgttac tgcaggcttt gatgaaactt atacagaaac gaaaaaaaag
    20221 taagcgaagc tctcaaatat ccttttttta aacattcgat tacttatcga gaattagatt
    20281 taaagacgca attactcgaa gcaggttgga cgttaccaga aattgaacgt actgactttg
    20341 acgagttaat gcgtcttttt gcttttcgag atgcagtaaa agagcatgag gatgttgagt
    20401 actacgataa cttcacccaa ttttaggagg tgatactttg aataacgatg acctaattct
    20461 gaagatgata ctagatgaat ctggttttac tgccggtatg aataatgctg tcaaaaagtt
    20521 gaactctttt gatgaaacaa tcgaaagaaa aagtagaaat agtggcagtt cattaggtag
    20581 tatttggaaa atatttgctg gaagttttct agctagcgga gtaactagaa tcgtaggagc
    20641 tggctttgat ctaatcaagg gttccataag tggagcggtt gatcgagtag atacgatgaa
    20701 taacgctcta cgaaatttcc aaaatatggg atttagtaat tcagaaatta tgaagaatat
    20761 cggaaagaat gggcttttat ctcaaggtat tcaaggactt cctactgctt tgaatgatgc
    20821 gataagtcat gttcaacttc ttgcttcttc tacaggtgat ttaactcgtt caactcaaat
    20881 atttaaagct ttaaatgatg gaattcttgg tttcggtggt tcaactgatc aagttaatga
    20941 agctgttatt caattatctc aaagtttctc aaacggaaaa gtagacgcac aaacttggaa
    21001 ctcaatgatc aacgctcaac ttggacctac tctttctgct attgctaaaa agatgggaat
    21061 tacaatggga gatctgaaag aaggtttatc tcaaggtaag atttctgttg aagaattcca
    21121 aaatcaatta atagaaatgg ataccaaagg tggcggagga cttaaatcat taagtcaaat
    21181 cgctaaagat tctactaagg gaattaaaac ctctatacaa aatgcaaaaa cagctgttac
    21241 acgtggtgtt ggtgaggtaa tagaaggatt aaataaagca ttagtagatt cagatttagg
    21301 tggatttaag ggaattattg ataaagtggg cagttcaatg gaatcgttct taaaagtaat
    21361 tgctgcaaat attcctacag cagtatcatt tttaagtaat ttatttaacg aaattcaaaa
    21421 atttggttct gctttgaaat tcatgatgcc ctttcttgtt cctgcagcta ccgcttttgg
    21481 agcgttcatg tttcaactta aaggtatacc agcaataata aaaagcttca ataattttaa
    21541 gaatgccata attggcgttg gaaattcatt aaagattatg ggggcaatag ctgctgctaa
    21601 ttcatttgtt ttaattattg gtgcagtcgt tggtgcaata gctgttttcg gttactttat
    21661 ggcaaccaat gaagagctta gaaacaaggt tatatctatt tggaatgatg tgaaagattc
    21721 cgtaattggt gtattaaaaa atataaagga ctggggaatt gatacttgga attctgctaa
    21781 ataaatggca tcaaatgcag ttgagggtgt caaagatgct tggtcaggaa caaaggaatg
    21841 gttttctaac acctggaaag gtattaagga tggggccaca ggtttatttg ataaaacagt
    21901 agagacttca agaaatgcag tcgatagtgt aaaaaatgca tggtctaata tgaagaaatg
    21961 gttttctgat acttggcaaa gtataaaaga ttcagccaga gaaaaatgga atggaatcaa
    22021 aggttctatt atgtcagttg ctggtccatt gattacaggt ataaagaatg catttttaca
    22081 tgttactttt tatctagaga ccttatggaa taatttagtt gagattggta aaaacgtctt
    22141 tgagatttta aaaaatgtta tacttgcgcc ggttttgttt attacctcca tgatttctgg
    22201 tggatgggaa gaaacaaaaa ataatatgat tgctgtttgg aataatatta aaga
//
LOCUS       DAYYME010000037        22165 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000037 DAYYME010000000
VERSION     DAYYME010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.037"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            230..2065
                     /gene="tdc"
                     /locus_tag="ACHBFW_001181"
     CDS             230..2065
                     /gene="tdc"
                     /locus_tag="ACHBFW_001181"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM9317500.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2370..3848
                     /locus_tag="ACHBFW_001182"
     CDS             2370..3848
                     /locus_tag="ACHBFW_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9317501.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3814..5145
                     /locus_tag="ACHBFW_001183"
     CDS             3814..5145
                     /locus_tag="ACHBFW_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9317502.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5259..7811
                     /locus_tag="ACHBFW_001184"
     CDS             5259..7811
                     /locus_tag="ACHBFW_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFM9317503.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7818..9257)
                     /locus_tag="ACHBFW_001185"
     CDS             complement(7818..9257)
                     /locus_tag="ACHBFW_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9317504.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9232..9414
                     /locus_tag="ACHBFW_001186"
     CDS             9232..9414
                     /locus_tag="ACHBFW_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317505.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9603..9965
                     /locus_tag="ACHBFW_001187"
     CDS             9603..9965
                     /locus_tag="ACHBFW_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFM9317506.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10152..11009)
                     /locus_tag="ACHBFW_001188"
     CDS             complement(10152..11009)
                     /locus_tag="ACHBFW_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9317507.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11129..12040
                     /locus_tag="ACHBFW_001189"
     CDS             11129..12040
                     /locus_tag="ACHBFW_001189"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM9317508.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12047..13698
                     /gene="mdcA"
                     /locus_tag="ACHBFW_001190"
                     /pseudo
     CDS             12047..13698
                     /gene="mdcA"
                     /locus_tag="ACHBFW_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            13800..14099
                     /locus_tag="ACHBFW_001191"
     CDS             13800..14099
                     /locus_tag="ACHBFW_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFM9317509.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14089..15765
                     /gene="mdcD"
                     /locus_tag="ACHBFW_001192"
     CDS             14089..15765
                     /gene="mdcD"
                     /locus_tag="ACHBFW_001192"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFM9317510.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15769..16362
                     /locus_tag="ACHBFW_001193"
     CDS             15769..16362
                     /locus_tag="ACHBFW_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFM9317511.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16386..17462
                     /locus_tag="ACHBFW_001194"
     CDS             16386..17462
                     /locus_tag="ACHBFW_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM9317512.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17487..18356
                     /gene="mdcB"
                     /locus_tag="ACHBFW_001195"
     CDS             17487..18356
                     /gene="mdcB"
                     /locus_tag="ACHBFW_001195"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFM9317513.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18467..19231
                     /locus_tag="ACHBFW_001196"
     CDS             18467..19231
                     /locus_tag="ACHBFW_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFM9317514.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19720..21813
                     /locus_tag="ACHBFW_001197"
     CDS             19720..21813
                     /locus_tag="ACHBFW_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9317515.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21838..22140
                     /locus_tag="ACHBFW_001198"
     CDS             21838..22140
                     /locus_tag="ACHBFW_001198"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9317516.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7179 a         3787 c         4722 g         6477 t
ORIGIN      
        1 ctttttttag gttatctatt ttagaagact gatttattga aaaattgttc gcttttttgc
       61 gttgaattat cagaaaaatc cgactttatt tatttttgtt tgtaaaaatc tacgtggatt
      121 tgatgttata aaaaaaaaat tctcattttt ggcgctatag tgaagaggtg ttacagataa
      181 acacatgtat ctgcaagaca ttgaccaaca aaatatggag ggaagaaaaa tgaaagatat
      241 ggatatcaag gccgtcttta taggcgacaa agctgaaaat ggaccagttt ataaaatgtt
      301 gttgaacaag atggtagatg aacatcttgg atggagagaa aattatattc cttcagatat
      361 gcctgcaatt agcgaaggag ataagctcac tccagattat ctagccaccc gcgaccatat
      421 gatagaggtc ttggatgaag tcagtcagcg attacgcgca ggatcgatcc catggcattc
      481 agcaggccgt tactggggac aaatgaatgc tgaaacattg atgcctgctt tattagctta
      541 caactatgcg atgctgtgga acccaaataa tgttgcattg gaatcttcta tggcaacttc
      601 gcaaatggaa gcagaagtag gacaggattt tgcttcatta ttcaatatga cagacggatg
      661 gggacacatt gcagcagatg gttctattgc taatcttgaa ggattatggt acgcccgttg
      721 catcaaatcg attcctttag ctgtcaagga agtgctgcct gaaaaagtga aaaaaatgtc
      781 tgagtgggaa cttttgaatt tatctgttga agaaattttg gaaatgaccg aaagctttac
      841 ggacgaagaa atggatgagg tcaaagcagc ttcttcccgt agcggaaaaa atatccaaag
      901 attagggaaa tggctggtcc ctcaaacaaa acactattct tggatgaagg cgttagatat
      961 ttgcggagtc ggcttagatc agatggttgc tatcccagtt caagaggatt atcgaatgga
     1021 tattaatgct ttggaaaaaa caattcgtga attagctgga caaaaaatcc caattctagg
     1081 tgtggttgct gtcgtaggaa cgacagaaga aggtcaagta gacagtgtag ataaaatcgt
     1141 ccaattaaga gaaaggttaa aagatgaagg gatctatttc tatttacatg ttgatgccgc
     1201 atatggcggc tatgcgcgtt cactgtttct aaacgaagca ggagaatttg tgccgtatgc
     1261 ttccttagct gaattctttg aagaacatca tgttttccac cactgcgtaa cgattgacaa
     1321 agaggtatat gaagggttca gagctatttc agaagcggat tctgtcacga tagatcctca
     1381 taagatgggc tatgtccctt atgctgctgg aggaatcgta atcaaacata aaaatatgag
     1441 aaatatcatt tcttatttcg cgccatatgt gtttgaaaaa tcagtaaagg ctccagatat
     1501 gttaggagct tatattttgg aaggatcaaa agctggagca accgccgccg cagtctggac
     1561 agcacatcgt gtattgccat tgaatgtgac cggatatggt caattgattg gggcttcaat
     1621 cgaggcagct caaagattca gagaatttct cgatcatctg acttttactg ttaaaggaaa
     1681 aacaatcgaa gtctatccat tgaatcatcc tgattttaat atggtcaatt gggtattcaa
     1741 agaacaaggc tgtacagatt tgaacgctat caatgaatta aatgaaaaaa tgttcgatcg
     1801 gtcttcttat atggatggcg atgtttatgg tgaacggttt attacttctc atactacatt
     1861 tacacaagaa gattacggtg attctccgat tcgttttgtt gaaagaatgg gtctaacgaa
     1921 agaagaatgg aaaaaagaac agaaaatcac tctgctacgt gcggctatca tgactcctta
     1981 tttgaatgat gatcgaattt tcaattttta tacaaaaaaa atcgccaaag caatggaaaa
     2041 gaaactaaac gaaatcatcc aatagaactg atttttgtgt gaaagaataa caagagacat
     2101 atggagaaca gacaattatt tgcttcatca aaacaattgt atttccccct gtttttcata
     2161 tattcctcgc caagatattc ttttttataa aacgttctat aagttggatc gttcgtttca
     2221 tgtatcaaac ccagttcact aagatcgttc aatggatgtt tcggtgatac tgggttttgt
     2281 actgaatcga tgaaaccttg tcatttctgt tcttgaacaa aaataaaaga aaacaaaaac
     2341 atatgtcgct attgaaagaa ggaataaaga tgaaagatac tggttcatca aagcaaatca
     2401 cgaccatgac ctttatcgga atgacctgcg cccttgtagc aagtgtacga aatattccag
     2461 atgtagcagc aacgagctgg acgatgattt tttacatgct ggttgcagta ttattgtacg
     2521 cttttccaat ctcattgatc tctggtgaat ttgctggaat gtttccccaa aaaggtggtc
     2581 cagaactttg ggtatcgaat gctctaggaa aaaaatgggg gttcgttgtc tcatggctgt
     2641 tatgggtcca aatgtttcct gggatggtga tggtcgcttc agcactcgct ccgttgtttg
     2701 gtaatattat cgataatgtg ccgcttggtt tgaacagcaa atttacatta gttgtcattt
     2761 tagtcgttta ttggatcatc acttttttga atttgaaatt tgatatggca aaaatcggcg
     2821 gaaaagtcgg tgtttggtta ggattgtacg tgcctttgac gatgatgctc ttattagggt
     2881 ttgctgcgtg gatcaaagtt gggatcgtac caaccggaac attaggcagt ttttcatggg
     2941 acaaattaat accagatact acgactgcat cctcatttgt ttattttgcg ccgatcatgt
     3001 ttatttttac tggtatcgaa atgtcctctg tgtatatcac tcgattggaa aatccagtta
     3061 aaacgtatat tcgcggagta tttgccgcgt taatttttat atttttcgtc aatattttga
     3121 atgctttagt tgtagcaaat gtcgtaccaa aaggacaaat ggaattgaat aatatcgcgc
     3181 aatctatttc gatttattgc caaattcttg gtttgccaca tcttattgtg aatctgttta
     3241 gtttacttgt cttcattggt gtagcagtcc aattatctgc ttgggcctca ggaccagcaa
     3301 agacagtgac agaaagtgct agaaaaggtg cgtatccacc aaaattcaat ttttggaaga
     3361 cgaatcaatt tgacgtatcc aaatccgtta ttttgactca gtctgtcatt atctcaatct
     3421 ttgcactttt ttatttgctg attcctggcg tcaatcaagc atttttgatg ctcgtaaatt
     3481 caactactgt tatctactgt atcgtctatg tcatcatggg gatcgcagtt ttgagattgc
     3541 gttatacaca tgcaaagttg aatcgaccat ttagaattgg aaaaaaagag aaaaagagca
     3601 atcttggagt ttggactgtc gttattgttt tatttgcggc aatcggattt tctgtcggac
     3661 tgacgatgaa agctggaaca tggatcaatc taattgcagt cacagccatc agtgtcattt
     3721 tgtttgtagt accactctgt attgaaaaaa taaagaaacc ttcttgggaa caggaagtcg
     3781 ccatgctgct tgaaaaagaa aaaggtgaac ttgatggaaa aaccggcaat gaaacggact
     3841 ctaactaatc gaaatatcca gttaatcgca ttaggtggcg caattggaac aggattattt
     3901 ttaggttcag caaaggcaat tgcatcagca gggccagctg ttattctcgt ttatttgata
     3961 tgcggtttgg ctatttatgg tttgatgcgg gccatgggtg atttattttt agcaaatagt
     4021 acatttaact ctatcagtga tttcgttgac tattatttag gcaaaaagtg ggcatttttt
     4081 gtcaattgga cttattggct ttgctggctt ggagctggac ttgcggaatt aacagcaata
     4141 ggattgtata tccatttctg gtttgtacat gtaccagctt tgataagcgg aggtagcgca
     4201 ctaatgatct taatggcagt caaccttgtt gcagtcaagt ggtttggtga attggaatct
     4261 tttttctcaa tcatcaagat cttggggatt ttccttttta tcttattggg tataggattg
     4321 agtgtctcag attttcatac ccagtctatc ggaatcaaag aaaattttgg acagatcgat
     4381 gaattactac cgtttggctt tgctggaatg gtagtctcat ttcctatggt tttattcagt
     4441 tttgctggaa ttgaaatgat cggcttaacc gttggcgaga caagtgatcc gcaaaagaat
     4501 ttgaaaaaag caatcaactg tctgccttgg cgtattctct ttttctatat aggaacgatt
     4561 ttgtcgattt tatttgttgt tccatggcat tgtttagatt taaaagaaag tcctttagtg
     4621 actatgctgc aagtgatcca ttgccaaggc ggagcagcaa ttgtcaatct aatgattttg
     4681 gttgcttctt tgtcctcagc aaatagtgca gtctacagta ctagcaggat tttatatcaa
     4741 acagcaaaag aaaaaagttt gccccattgg ttttgtgcgc tttctaagaa aggcgtacct
     4801 ttacacggta ttttaattac aggaatgtta ttttttgccg gtctttgtct acattttttt
     4861 gtagctgata gtcgattgtt ttttcagctt ttggcaggca tgacaacgag ttgttttcta
     4921 tttgtgtggt cttcgatctt gtgtgcacat gttcaattta ttaaagaaaa gaaacggaac
     4981 actacacaaa gatataaaga tcttgctttg ttggtgtttt ttacttcaat tgctttcagc
     5041 ttgttattcg aaagcacgac acgctttatt ccttttttat tggttatctg gtttgtattc
     5101 ttaaattttg tatataaaag aataagcaat agaaatatca gttaggccca gatacatttt
     5161 gcaaaacgaa ttatttcaga cccccctgaa ataatcgata cgcctgaaaa aacttccctg
     5221 cgtataataa cagtatcaat taaacaggag gggataggat gaactggtac agattgacgg
     5281 tagaagatgt attgaaaaac tatcaaacat caacagaagg cttgtcgcaa gaagaacgac
     5341 agaaacgcct gactgatcaa ggatataatg aaataaaagc aaggcagcag acaaaacgat
     5401 ggaaaaaaat agcaaagcat tttactgatc tgctgatgat cgtgttgatc attgcctctt
     5461 ttttgaaatt tgcttctagt gaatatattg aaggaagtat tattttattc gtagtgatcg
     5521 tcaatggact cgttagttac tggcaagaaa gaaaagcgga agaatcattg aatggtttga
     5581 agcagctgat gggacaagaa gcgatagtac tatcaaacgg gatgcaagaa aaaatccctg
     5641 cgaaagaact ggtaaaagga gatgtcattg tcttacgtgc cggagatgtc gttccagcag
     5701 atatccgatt aatcgaagca catgatttat tagtggaaga atcgattttg acgggagaat
     5761 cagaagcatc tgaaaaaagt catgccgttt tgacagaaga agaatcgatc ggcgaccaga
     5821 aaaatatggg tttttcagga acgcttgtac aatctggttc tgcagtcggt gtcgttgtag
     5881 aaacaggaaa cacgacagaa atcggtaaaa tcaatcaagc attgcaatcg gtcgaacaac
     5941 aaacgacgcc tctgattaaa aaaatcaacc gattaaacaa acaaattttt caaggcattc
     6001 tttgtttgag cctattctta gttattttta cgacatttcg atatggtatg gattggcata
     6061 tccttctttc ggctacgatt gctttggtgg tgtcaatggt acccgaagga ttgccagctg
     6121 ttttgaccat gattctatcc gttggagtac atgaaatggc aaaagaaaag gcgatcatca
     6181 aaggacttcc ttcggtagaa acattaggtt ctatgacagt gatctgttca gataagacag
     6241 gaacattgac aaaaaatgaa atgacagttg tggatgtagc tgcaaaagaa gaagcctgcg
     6301 tgttgtccat tatgaaaaac tgtcaggaat taaaaacaaa agaagaacag aaaacagaaa
     6361 atttaagtgg caatccgaca gaagttgcgc tgattcgaca cacagaaaca gccaatctac
     6421 ctttgaaaaa aatagaagca aagattccgt tcagttctga atacaaatat atggcaacga
     6481 tgcatgcgga ggaaaaagga gcggtgattt acgtcaaagg tgcaccagaa gtattatttg
     6541 ctaaatcaac tctttcttct gcagaacaag aagcatggag tcagacagct gcagaatttg
     6601 cgagaaaagg tcagcgtgtt ttaggatttg cttataaaaa agtagattcg aaacaggaac
     6661 tcacgcatga gactttaacc caattaactt ttgcagggat cgccggattg atcgatccgc
     6721 caaaagaaag tgcagtcaaa gctgtaaagg aatgccaaca agcagggatc tcagtgaaaa
     6781 tgattacagg ggatcacaaa gacacagcaa aagcgattgc ggaacaaatt ggcttaaaac
     6841 atactgccaa agttttagaa gggatcgatt tggatcttat gtctgatgaa gagttgattc
     6901 agcaagtgcc aatagtcgat gtctttgctc gaacgacacc agaacataaa ctgcgaattg
     6961 ttaaagcttt gcagaaaaat ggagaaatcg tcggaatgac cggagacggc gtaaatgatg
     7021 cacctgcttt gaaaagatcc gatgtaggaa ttgctatggg aatcaaaggt agtgaagtaa
     7081 gcaaacaggc agctgatatg gttttaggag atgataattt ccatacgatt gcaaaagccg
     7141 taaaagaggg ccggcggatt ttggataatt tgcaaaaaac catcaatttc ttcttgccaa
     7201 ctgcattagc tcaaggattg atcttgattt gggcacttat gctgaatcgc ccgctacctt
     7261 tgtccccagt acagatttta tgggtcaata tggtaacgac aatcacttta tcttatgcac
     7321 tagggttcga gtcgccagat ccagaaatca tgaagcgccc accaagagat ccaaagcaag
     7381 gaatcctatc tggttatcat ttgtttcgga tcatctatgt ctccctactg atcatggttc
     7441 ctgcctattt attggctatg cagtttgaag ggcaaagctt acagcaaacc attcttctgc
     7501 aaaatatcgt cttagctcaa gctgtttaca tgatcaattg cagagagttg tccaagccgt
     7561 cattgaacca aggtcttttg caaaacaaag cgctttttct ttcattaggt atccttgctt
     7621 tattgcaagg gatggttatt tttctaccag tcggccagca gctgatcggt acagtaagtt
     7681 taacgatgac acaacagtta ttgattggag caaatgtgat tcttctattc ttagttgtag
     7741 aaatagaaaa agggatcatg aacaaactgt ttcgcaaaaa agaaaaaaca gctagagaat
     7801 acagtcgcta gccgttacta taaaaaagtt ttgaaagtga gttcagtcaa acgattgagc
     7861 tcatttttcg ttggaataga agaaatgtat acaataggga tatctagaga atctagaggg
     7921 aaattggaaa tcaagatatc tgttttttca aaagaaatag tggaaagatc ggttagttcg
     7981 ataggtaaga agcagacacg ttttcctaag taatctttta attcctgctg caagaacgcc
     8041 ttccatgaag gttctccttg gaaaataaaa tagacattga acaaattcgg tttgatagaa
     8101 aaaatcgttt gttggatcag tagagacaag agttggagaa agtaatttgg ttcattgatt
     8161 tctatttctt tttttagatc gttcagtaaa ttctctttct gttgatacaa gtgtgcatat
     8221 tgctgatcat atgtttctaa aaaacgatgg tattgacggt ccaatgcttc aatcaatatc
     8281 ggcgattcat gatattttaa tatgaggaga gccaagttat cagtcagaga tgtttttttt
     8341 agttgatcaa gagacagctc tgaagctagt tcctctacaa acgtttcgag gacctctaaa
     8401 aatggagaat gatgcaggca gcgagcggtt tcttgtccat agttattgtt gtaattccaa
     8461 ctttccagaa agcagaaaga agcaaaagaa agctcagtgt ctttaagata aataccttcc
     8521 gttaaataca attttttcaa tgcagagtca tacagggagg agagtgtgct gttggtatgg
     8581 atagaagaat ctaatcggca attcgctttt atacggatcg tattgataaa gaaccaacat
     8641 gttccgaaaa tctcttctgt ttcaaccgct agttcagttt gactgaggtt tcttaaaaat
     8701 tgaaaataat gggattcatg gatcgaataa tcctcgaaaa aatagttgtt atgagtgaaa
     8761 ggaatcagca atcgatgata aaaagtacga atcgcacgct cttttccaag aatacaagct
     8821 gtatatttcg tcaaatcgat ctgcaagtcg taacttgcta aacgctgatt gattttagtc
     8881 acatgtcttt tcaatgtccc ataagaaagc ccatggtcgg ctagaaaatc agggacagca
     8941 actttttttg taaaaagcag atttttcagc agttcgacac tgactgattg tttgaaaaaa
     9001 atctcccaaa ggtcatttgt taataaattt tgctggtttt gcaaactgat tcctgcttga
     9061 tcgctgatga tgtcccagcc ttcgggcagc tgagagcgaa tagattgaag gtcagaaaaa
     9121 atcgttcgtt cggttgtccc tatgatttca gctaattcat ttacggttat ctttgtatga
     9181 ttcagcaatt gttctactaa agaaacatga cgtcgaatat ctttttcggt aatgattttt
     9241 ttcattagtg aatacatcaa tcagactccc ttctattcta agaatacgga gaaaataaac
     9301 ggtggtcaag tttcaactga tttttcaatc attgtttatt tttctggagc atttactgat
     9361 gataagaatt tcatcatgaa cttgaaaaat gataccacta aaatcaaaaa ataggtgggc
     9421 aaacaataat aaacatttta atagtagatg gtagcaagac caacacaggc ggattgaaca
     9481 tttaagagtt gataaaaaag aaaaatatcg aagtatttga ttagcaacta cttgtattca
     9541 tttttctatt cttacgtggt atctttgaat aaagcgaatc aataagaaag agatggaagc
     9601 agatgctaac gaaaaaagaa ctaatcgatc acatcaatga aacatacaat ttgtctccag
     9661 aatatatttt taaaaaattt cctgattatt gcgtattcag acatcaaaaa agcaaaaaat
     9721 ggtttggggt cctgatgtct gttccagaag aaaaaatcta tgatgatgca agtgaccata
     9781 ggatcatcaa tattcttgat ttgaaggtcg atcctgaact tggcggaatt ttgcgaggaa
     9841 aagaggggta ttttccagcc tatcacatga ataaagaaca ttggatctct attgatctac
     9901 gtaaaataga aaagctagat gaactagcag atttgattga tggaagtttt aaatcaacgc
     9961 aatgaatgtc taggacaata gagaagaggt tgtgacagaa atgtttagct tcaagaaata
    10021 agaaggaatt tacgaaaatt gcttctcaaa tttttgggaa atttcggctt attttctaag
    10081 aaattacttc tggaacaccg tttgttcgaa aaaagagctg cgacaagaat tttgtcccag
    10141 ctcttttttt tttattttcc taaataaaat tcccgcatcg tttttgcaat cattttgatc
    10201 aaaggatgat cttctgtgaa gctagaagat aaataaattg ttcgtgtcaa gggcaaatct
    10261 ttgatttccc gcaagacgaa aggatcattt ttatcgtaag accaagaaac ttttgggaaa
    10321 aagctaacag ctaatccttg cttgacaata gaacgtaatg tcgcaggatc atctgtcgtc
    10381 gaactgatcg tcaattcgat acttttcgct tcaagtgctg catccagtgt atgacggaag
    10441 gcgttgctct ttgacagcat caagatgggg gtgcgttgga tatcttctaa ttgcagatgt
    10501 tcttttttag caagaggatg tttcttagga atggcgacat aaacaggttc tttcatgata
    10561 ggaatattgg tcatgccagg cttcatctcc gaagtgatgt acagtacagg atcggtaata
    10621 tcatctggta aatgatgttg catgatcgtt aattggatat caggatgctt ttccataaaa
    10681 attcggacca agttcggaat agaaacagag gctacctcta aatacaatat gacggaagta
    10741 acgatcttct cattgaccga agacaataat ttgattcctt cttctaatgt gtctaatgtc
    10801 ttgactgcat atggataaaa ttcttctgca taaacggtcg tctcaatgtt tctgccgcta
    10861 tggtaaaaca aagggacacc taattcgttt tccaaatgtt tgatcgcacg gctcaaggca
    10921 ggttgagaaa taaataattc tttggctgct tgggtaacat tttttgtttc gaatactttt
    10981 ttaaaatact gcagatgaag caatttcata attatccatc ccatccatac ctttttgtta
    11041 tggttttttc tttttttggc attttttatt atagcagaaa ttgactaaga tagcagtgga
    11101 aaaagtaagt gctgaggagg cgaggctcat gagtacaggt ttcttatttc ctggacaagg
    11161 cagtcagaag ttagggatgt tagcggatat ccctgaaatg tacatggaac gtttagaaaa
    11221 tgtgacgggg tatcggttag ttgataaaga agaaaactat caagatacgg tttttatcca
    11281 gctagcatta ctgacaaaag cagtatttta tttagatgaa atggaaaaaa acggtattca
    11341 gcctgatatt gcagcaggac attcgattgg tgctttttgt gcagctgttg cttgtcgatc
    11401 tttgagattt gagaatgcag cggaactagt ctatcaccgc gcagtcttaa tgaaagaagc
    11461 ttatccaagc ggttatgcaa tgggcgtagt agtaggtgtg acgagaagcg aagctgagga
    11521 aattgtagca aaaacatttg atcctgacca tccagtttat ctgtcaaatg aaaattgtcc
    11581 gatgcaacat acgttatctg ggagtatcga aggtttatcc gttacgttgg cagaagccaa
    11641 acggcaccaa gcgcaaacag cgaagctgct gaaagtccct gtgccatctc actgtctctt
    11701 gatgaatgat acggtcaaga aattcaagcc gtatatgcag tctgtccatc tatctgatgc
    11761 tagatgtttg tatataaaaa atacggatgg aagagccact caggacgcag aagagattcg
    11821 taaagatttg ttggaaaacc tagctttccc tgtgcagtgg aaccaaatga tggacttggc
    11881 aaaagaatta ggaatggatc tgacgattga atttcccccc ggcaatactt tgacaaagct
    11941 gattcataca aaatttggtg tagataaagg gatccgtacg atcaatctag accaacatgg
    12001 tattgatgac gccacgtttc tatatcaaaa atggagatga ctaattatgg aaaaaccaaa
    12061 ccgcaactgg gcttctaaac gcgaaaaaaa agcgcagcga ttggaaaaaa tcagcaactt
    12121 gctcaatggc aagtatgtag aaactgaaaa aatcgtagaa gtaatggaaa aactgattgc
    12181 tcccggcgac aaagttgtat tagaaggcga caatcaaaaa caagcaagtt ttctttcaga
    12241 aagtcttgaa aaagtagatg cagaaaaagt gaatgatctg catttgatca tgtctagtat
    12301 ctcacgacca gaacatttga atatttttga aaaaggaatc gcaagcaaga tcgatttctc
    12361 ttatgctggt gcacaaagct tgcgtgtcgc ccaaatgatt gaagacggca caatgaaatt
    12421 aggtgatatc catacttatt tggaacttta tggacgctta ttcatcgatt tgattccaaa
    12481 tgttgtttta gtagctgcag ataaagcaga taaagacggc aacttgtaca caggtttcaa
    12541 tacggaagaa acaccaacaa tcatcgaagc tgcagctttc aaagatggta tcgtgatcgt
    12601 tcaagttaac gaagttgtcg attctttacc acgtgtagat atccctggtg actgggtaga
    12661 cgttgtcgtg aaagcagata aaccttatca attagaagct ttattcacac gagatcctca
    12721 aaacatcaca gaattgcaaa ttctaatggc aatgatggca atcaaaggaa tctacgcaga
    12781 acacggcgta caatctttga atcacggtat tggattcaat acagcagcta tcgaattgtt
    12841 actgccaact tatggtgaat cactagggtt aaaagggaaa atcgcgaaga actgggtatt
    12901 gaacccacat ccaacgatga ttccagccat cgaatcaggt tgggttgaaa gcatccatag
    12961 ttttggtggc gaagttggta tggaaaaata tattgctgct cgcccagatg tcttttttgt
    13021 cggtaaagac ggcacgatgc gcagtaaccg aacattatca caagctgcag gacaatatgc
    13081 catcgatatg tttatcggtt caaccttgca attagattac gaaggaaact cttctaccgt
    13141 aacaaaagga cgtttatctg gttttggcgg tgcgcctaac atgggacata atcctggcgg
    13201 tcgtcgtcat tctacaccag catggcaatc acttcgcgac aatgatgatc cattagggaa
    13261 aggccaaaaa ttagttgttc aaatggttga aacatatggt tcaaataaaa aaccagtctt
    13321 tgtcgaagaa ttagatgcac tagctgttca aaaacaagca ggcttagcca atgcaatgca
    13381 ccagtgatga tttatagcga agatactaca catatcgtga cagaagaagg aattgcttat
    13441 ttgtacaaag caaaaacaaa agaagaacga caagcagcaa ttgcagcaat tggcggtgtt
    13501 acacctcttg gaatgactag cacgaaagaa tcactagata gcttgcgtgc agccggcatc
    13561 gtgaaattac cagaagatct aggcatcaaa cgtagtgaag cgaaacgttc attattagct
    13621 gcacaaacca tcgatgattt gatcgaatgg tcagatggcc tgtatgaacc accaatgaaa
    13681 ttcagaactt ggtcataaaa ttggcgagaa aaaccttttg tcgccattta cccagatgga
    13741 agaagaaaga cgaataagca gaacgtgtat gctctgtttt acaaaaaggg gagagatcca
    13801 tggaacaatt agcttataaa tatccaacga aacagccgat caataagaaa atccatgtag
    13861 gagtggtcgg ttcaggagat ttggaaatct tgatgtttcc aacagaaaaa acagaatcta
    13921 ctgtaaaaat ttgtacagga agcgatggct tcggcgaagt atggaacaat gtattaacga
    13981 gattttttga tcgttatcca atttcagcag atatcgtgat caatgacttc ggtgctacac
    14041 caggagttgt gtacctacga ttgacgcaag caatggagga actttcagat gaaaaatagt
    14101 tttgtagaat taaacgccag agaacgagtg aacgctctac ttgacaatgg acgtgaattg
    14161 attgatccat tcgatcaaat catcgcgcca agtttaccag cacaaggaat cgtccctgaa
    14221 agtgatgacg ggatcgttgt cagccgtggc acgatcaatg gcaaacaagc agtagtaatc
    14281 gctatggaag gaaaattcca aggtggcggt atcggtgaag tttccggtgc aaaaatcgtt
    14341 gctgcacttg aacatgtgtt agaagaaaat aaaaaaggaa atgagatctt tccagtcatt
    14401 gtactagata caggtggtgt tcgtttgcaa gaagcaaact acggactgtt atctatttca
    14461 gaaatcggaa atgtaatcat tgctctaaaa caatatgtac cagtcatcgg tttgattcca
    14521 ggacgtgtag gatcatttgg cggtatgtcg attacttcag cattaatgag ttatttaatc
    14581 gcaacaaaaa aagcgagagt cggtttgaat ggaccagaag ttattgaaca agaagcaggc
    14641 gttcgagaat ttgattctag tgacaaagac ttgatttgga atacattagg atctcgtcaa
    14701 cgggtagaat caaaaatcat tgatgaatta gtgactgata gtgtagaagc aatcaaagct
    14761 gctgtcatcg cagcaatgga cgaacgaaaa gacacgcatc gcagtgaaag ctcagcattt
    14821 tatttatcat tacttgattc tttagatctt tcaaaaccaa tggccattga cgattacaat
    14881 gaagcaattg ctaaccatca agcaaaatca attgacttac cagaagtagc agaaggaaat
    14941 gaaccagctg cagcaagtgt tggttacgat tggtttgcgg cactaactgg catgaaaaat
    15001 ccaacttctt ctattagtac tgtgtattat ggtaaatcaa gtaaattcat agaagaagcc
    15061 atcgtgacat caatcgtacc tgatcctaaa aacccgatgt accgtgtacg aaacggtgaa
    15121 gttggtttag ttgaaggttt ccgcatggga caaattttgg atcacgttta cgaagaagat
    15181 aaagacaaaa aagtgaaacg gccaatcatc gttgtgatcg atgtaccaag ccaagcatac
    15241 ggctataacg aagaattgat cggtatccat gtggctttgg ctaatagtgc tgctgcttat
    15301 gcaaaactcc gtcaagctgg acatccagtt attggattga ttgcaggaaa tgctatttca
    15361 ggtgcatttt tagcacatgg attacaatca agccgcttga ttgctttaaa caataaagaa
    15421 attaccgtcc aagcgatgtc taaagaaagt gctgcacgtg tgacaaaacg ttcggttgct
    15481 gaaatcgaag cagctgctga aaaagttcca gctatcgctt acgatatcaa taattataca
    15541 aaactaggag cgatctatca tttcctagaa gatatcaccg accaagctgc ttcagaacaa
    15601 aatgtcagca aagtcatcga agcaatcaat gaagccattc aagatgtccg caatacaaat
    15661 gacacgatgc tgacaaatcg atacacaaac gaaacagcca aaacattcgg acgtgtcgaa
    15721 acaaataaag tccgtgccaa aatggacgaa gaatgggatg cataaaaaat gattcaagca
    15781 catgatattg tcctttttga taaacatgaa atagaagggt tcaccttgcc tgaatgggtc
    15841 gttctaactg aaaagatgtt cggaacagtt cgtcgtgcgt cgacggatca cgcagaaata
    15901 ctaccgattg gtttacgtgg acctgcccgc aatcagagat ggtcaacaga aatctcaaaa
    15961 catgccgtca aacaagtcat tcatccgtgg gacatcatcg aacaagaatc gtatagacaa
    16021 gagaaaatca tggattatcc tgtttatcag caatttcatg ctgctagaaa catattatca
    16081 ggatgtaaat ggggaatcgg cggcagctta ggatttgaat tatcgactgg gattccggca
    16141 gtaaaagaaa ccagtgattt tgatttgctg ctgtacgcaa attcaccgat tgaattgcca
    16201 attcaggtaa ttcaatcgca tcctgctttt tttgagcaat tcgataccca agtagtgact
    16261 cggcaaggcg gtttttcact aaaagagtat ttgagagagc cagagaaaaa aattctattg
    16321 aaaacagtcg aaggacctaa attaaccaaa gaactttggt aataaagaaa aagagagaga
    16381 aagagatgtt tgaaaacaaa aaaatcaaaa tcaacgtaag tgacttccag aaagtatcag
    16441 ctgtaacggc tgttatgatg caaacgatat tgagctttgc tttggcaaac agccacagcg
    16501 caacaaatac agcttttctt ggtacctttt acgtcatcgt taaatacaca gcaccaatgt
    16561 ttatttttgc aattgtttat aatatggtaa aaacaagtca gcatttgtct tattgggaat
    16621 ttttgaaaga aaagtttttt gaactagtcg ttccgtatct tctatggacg acggcttatc
    16681 tttggctatt tccagcagtc caacaacaaa ctccgtatac ggatactgca agctttttac
    16741 tgaaatatgt cacaggtgat ggagcgcctc atttatggta taccgttatg atgctgcaga
    16801 tccaattgtt catgccgttt tttgtttggc tcggttacaa agtatttgct aataaaaaat
    16861 atgtatggcc agtattgatt ggttcaacga ttttatatgt cgcatggtat ctgttctatc
    16921 aaacacaagt attgactggg ccttatcatg acagctggta cttattagat cggtttgttg
    16981 ctagtttcat gatttatggg gtagccgcat ttgtttatca cgaaaaagtc tatcaatatt
    17041 tggacagagt acgttatctt ttcttacctg ttgccttagt tatcgcgttc ttttctgtcc
    17101 gttcattgct cgcacatcca ggagacttga gctttgcgaa tgcaccttac ttgaatacga
    17161 tccaaagttt atacagttta gtcatcatct ttgctgtctt cataggcgct tccaaaatga
    17221 ttgtaaacga ttcgccaaaa ttgccattgt tcaaatggtt gtctgtttat gcttatcgta
    17281 cgtatttagc caatgtattt gttttccaag tactgttatt attgtttaaa gatagttggc
    17341 tgcagttacc ttcaggagtc atgattcttg tagcttatct aatgacggca agttgtgcat
    17401 ttgctttaag ctggttactt catatcattt gggtagctat caaaaaaggt ttcagcaagt
    17461 aatagtagga agaaggagtg agtgtcatga taaatgacga acgaaaaaaa gcagtgactc
    17521 agcaactcgc tgcgttggta gaggaagcat tgatcgcaga agtaacatta agtccaaaac
    17581 ctggattagt ggatgcactg gatgcgggtg cacattccga tatggactat gctttatttc
    17641 ttaagtctgc taagacgcta actccttttt ttgaagaaat ggctcaaata gcatggcatc
    17701 atgctattga tcaggagtta agagaaagaa ttgctgagat tgggcgaaaa gcggaaaagg
    17761 caatgctgat tgctacaaat ggcatcaata cccataaagg agcgatttgg gcaatggggc
    17821 tattgatcag tgtcattgca caaaaaatca gtatggaaca gcctttttct ttatcgaaaa
    17881 tacttgatga tgtcagcaag cttgctgctt ttcctgatac aaaatatcgt cataaaaaaa
    17941 ccactcatgg tcatagggtg aagcaaaaat atggcgtaaa tggtgcatat gaagaagcga
    18001 tattagggta tccgcatatc aggcttgcaa ttcaagcagc taaggagcta gataatgatt
    18061 catatgtaca gaaacaaatg catatgttgt tagttttgat gagttcgcta gatgacacat
    18121 gcgtattgca tcgcagtgat cagaaaacgt tagtggaaat gcaaaaactt gctcatgctg
    18181 ctagtcaaag tgctttacca aatcgtccat ttacagcact gttgcacttt tgtcaggaaa
    18241 accaaatctc tcctggagga agcgcagatc tcttgtctgc aagcttgttc atgctgaaga
    18301 tagaggaatt atggactgcc gacacataca tttgtccgat gttactcaat gactaaataa
    18361 gacaaaattg ctaaaaaaac agtttatgca tctggttatt gactcttcct tttagcaagg
    18421 ctatcataaa aatagttttt tgtaataaaa gataacgagg aagacaatga aaaaaatcaa
    18481 ttatttagca atcattttgt tagtgggcct atgtttgttt gctctgccga cccaaggatt
    18541 cgctgtcgcg ggtggtcatg gtggaggtac atctagcggt cgaagttcgg gcagaggttc
    18601 atttggtggt cgttcatatg ggggcagtta ttttggcagt cgttcttacg gcagagattc
    18661 ttatgggtat tatggtccag gtacaggtcc ttctagttat gagcaaacgg gatttcttgc
    18721 attagctgtt ttgtcagtag gaggggtttc tgctttttct atcgtcaaga ataaaagaca
    18781 aaagaaaagg ctgaaagcgg agatattgtc ccgcatgtca ggaacggcta aagaaaagaa
    18841 agcacagttt ctggaaatcc aaaagatatt catgaagatc caatccgctt gggaagaaga
    18901 aactcctgaa aaagccagaa gctgttatac agacaagctg tttgccgaac atcagaaagt
    18961 tctgcaaaaa aacaaagcag acggtgtaag aaatcatact aaaaaaataa ctattcagga
    19021 agtaggaaat tatcgacaga tccacgaaaa tagttttgcc atccgaattg atttcagatg
    19081 ctgtgattat tcagtcgatc gaaaagatgg gcgattgatc tcaggaagta agacacaacg
    19141 acagtatttt tctcagatat ggtattttga tttttctgag aaagaaaaaa gatggcaagc
    19201 cgattttatt cagccgatta tgctggattg acaggaaata tttatgaacg gagttgtgac
    19261 aaaagccttg tcgcagctcc tttttctgaa taaacggtgt gccagagata gcctatcggc
    19321 aataaggcac gattttgtaa aatatgggaa gcgatttttc aaaatcgctt cttatcgctc
    19381 aaggctgaac atctctgtca caacctctcc actaaatggt gtttaaaagc ttgcatctgc
    19441 gataataaga acaaccaaac gaaaaatagg aagcactatt ttagtttagt tgttcttatt
    19501 acttgatgca ggacagcttt ttcacaacct cttttttgta tagatgggca aaatttttgt
    19561 aataataggc gtatgtcttt ttcgttgagg agatgaaaaa gcatatggct tattttttgt
    19621 ttgctttgct tactttcttt ccacttcaag tggcagaaat atctactttt tattatgtaa
    19681 gcggaaccat atacttgaaa tcagaaggag atcatttcta tgaaaacaag aacaattgaa
    19741 atacttcaca gaatcatgac agcccaatat ccagtaaaag caaaacatct agcaagtgag
    19801 ttcaatgtca gtcagcggac gatccgtcag gaagttcttg aagcgaatat ctggttgagc
    19861 agtaaaaagc ttccggagat ccgtacaatt cgcaacaaag gttttttgtt gaagctggaa
    19921 aaggaaaagc aagctcgatt agaaactgaa ataaaagatg tgaaagacgg ctttttgaat
    19981 cggcgcgaac gtatttttga tctagtttta gccgttgctt acagccgaat cccgatccat
    20041 ctaaacaaaa aagaagaaga atttcaaatc tctaaaagta cattagatga agatatgaga
    20101 cggttacgag caacactggt cagttatggg atagagatcg tcagttatgg aaagcaagga
    20161 ctggtttata aaggttcaga acgatctatc cgtacgatga tctatgattt tatcaatcag
    20221 aatctgggaa tgaccgactt ctttatgaat caagatgcac aattaacacc ggctcaaaaa
    20281 atctttctcc attattttcc tttggtggaa atcaaaaaaa tacaggagat ctattccaat
    20341 aagttgttga aacaagaaga tgatatttat aaaaaacaaa tcctgctttt tacattgatc
    20401 tggatccagc gaatcaggaa acatgaattt atctctgcca ttaattggaa aaatacagaa
    20461 ataaaagaca gtagcttttc tgcatttatt gaccacatga tcgaggaatt tcaattggac
    20521 aatgtcccgc aagtagaaag aaattatctt cgtttcacta ttgagacgtt caatgctaga
    20581 gatatctcca attcaatcga gtgggtacaa gctcaattac taacgatcca attgatccaa
    20641 tttgtagaga acgagacaca cgtacctttc catctaaagg aagaagcttt attcgaaaat
    20701 ctctataagc acatggctgc actgattgta agaatcaaga atcatatcca agtcatgaat
    20761 ccattgaaag aaaacatccg aacaaattat ttcccaatct accgcgcagt cactcatttt
    20821 gttcctacga tcgaagaagt aattggtggc aagatactgg aggacgagat agcttttctt
    20881 gtcatccact tttcgacgat cgccagtgta atcaagcgag acttgaatta tgtttataaa
    20941 tcagtcgttg tctgtaacca tgggatggct actggaaatc tgttagcaga gaatctgaaa
    21001 gaaaagtttc ctcagatcga gattactgca gttttaagtt caaaagaaat cgaattagta
    21061 gataaattgg atgtcaatct ggttttcagc acctttcaat tgaattatcg aaataagccg
    21121 cttttagttg tcgatccgat tttaactgag acgaatatct tggttataga agactttttg
    21181 aaaagaaatg aagaactaca gcggttagag cctaacagtc atgaatcgac ggagttattc
    21241 actcaaataa tagatgtcat gaaagaaagt ggtggccgag tagatcgaat catctatggc
    21301 aagttagaaa aattattcga aacaaacaat ttagaaatca acaaaaggga gatccagcct
    21361 atgttaaaag atattttgac ggacagcaat atcatgatca aagaaaaagc cactacttgg
    21421 gaggaatcga ttgaactagc tgctagacca ttggttaaag aaaacattgt cgaagagcgc
    21481 tatgtcgaag cgatgattcg tgcagtgaat gattacggac cgtatatcgt gattggcaaa
    21541 cacatggcac tagcccatgc ccgaccagaa gatggggtca ataaattagg agtcagcgta
    21601 gctacgattg agcaaccaat cgatttcggt aatcctgaaa tggatccagt aaagattatt
    21661 ttttgtttgg cagcagtaga ctcctactcg catctgaaca tcatgaaaga actaattgaa
    21721 ttgatcaaca acgaagagaa gttggatcga ttgattgaat gcacaaatat cgaagatttc
    21781 aaacaactgc ttttttcagt agttgagaaa taataaatga tacgggggag aacaaagatg
    21841 aacaaattga acattttatt cgtatgcggt gcaggtttag gaagtagttt tgcagcacag
    21901 atgtctgctg aggatgtttt gaacaaatta ggagtagaag ccaaactgga ccattgtgat
    21961 atttcttccg cagtttccat gaaaccagat gtgatcatca cagcagaaaa ttttcgttcg
    22021 cagttcgaaa agttcgcagt tgatcctaaa acggcgatga tctatttgaa aaatattgtt
    22081 tcaaaaaatg aaattgaaga aaaaatcacc ccagttctgc aagataaggg agttatctaa
    22141 atacaaaaag aaaagagggt aaaaa
//
LOCUS       DAYYME010000038        21904 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000038 DAYYME010000000
VERSION     DAYYME010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.038"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..577
                     /locus_tag="ACHBFW_001199"
     CDS             23..577
                     /locus_tag="ACHBFW_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9317517.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(750..1187)
                     /locus_tag="ACHBFW_001200"
     CDS             complement(750..1187)
                     /locus_tag="ACHBFW_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9317518.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     IKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1235..2182
                     /locus_tag="ACHBFW_001201"
                     /pseudo
     CDS             1235..2182
                     /locus_tag="ACHBFW_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            2453..2888
                     /locus_tag="ACHBFW_001202"
                     /pseudo
     CDS             2453..2888
                     /locus_tag="ACHBFW_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            2904..3737
                     /locus_tag="ACHBFW_001203"
     CDS             2904..3737
                     /locus_tag="ACHBFW_001203"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9317519.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            3982..5007
                     /locus_tag="ACHBFW_001204"
     CDS             3982..5007
                     /locus_tag="ACHBFW_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9317520.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5145..5270
                     /locus_tag="ACHBFW_001205"
     CDS             5145..5270
                     /locus_tag="ACHBFW_001205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317521.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            5295..6410
                     /locus_tag="ACHBFW_001206"
                     /pseudo
     CDS             5295..6410
                     /locus_tag="ACHBFW_001206"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            6493..7389
                     /locus_tag="ACHBFW_001207"
                     /pseudo
     CDS             6493..7389
                     /locus_tag="ACHBFW_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            7405..8229
                     /locus_tag="ACHBFW_001208"
     CDS             7405..8229
                     /locus_tag="ACHBFW_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9317522.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8261..9571
                     /locus_tag="ACHBFW_001209"
     CDS             8261..9571
                     /locus_tag="ACHBFW_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFM9317523.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9719..10978
                     /locus_tag="ACHBFW_001210"
     CDS             9719..10978
                     /locus_tag="ACHBFW_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9317524.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            10988..11860
                     /locus_tag="ACHBFW_001211"
     CDS             10988..11860
                     /locus_tag="ACHBFW_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9317525.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11872..12702
                     /locus_tag="ACHBFW_001212"
     CDS             11872..12702
                     /locus_tag="ACHBFW_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9317526.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12742..14925
                     /locus_tag="ACHBFW_001213"
     CDS             12742..14925
                     /locus_tag="ACHBFW_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM9317527.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            14986..16206
                     /locus_tag="ACHBFW_001214"
     CDS             14986..16206
                     /locus_tag="ACHBFW_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM9317528.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16203..17663
                     /gene="gtfA"
                     /locus_tag="ACHBFW_001215"
     CDS             16203..17663
                     /gene="gtfA"
                     /locus_tag="ACHBFW_001215"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFM9317529.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17740..18846
                     /locus_tag="ACHBFW_001216"
                     /pseudo
     CDS             17740..18846
                     /locus_tag="ACHBFW_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19168..19377
                     /locus_tag="ACHBFW_001217"
     CDS             19168..19377
                     /locus_tag="ACHBFW_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM9317530.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19370..19519
                     /locus_tag="ACHBFW_001218"
     CDS             19370..19519
                     /locus_tag="ACHBFW_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFM9317531.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19673..20338
                     /locus_tag="ACHBFW_001219"
     CDS             19673..20338
                     /locus_tag="ACHBFW_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9317532.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMSRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20622..21707)
                     /gene="xerS"
                     /locus_tag="ACHBFW_001220"
     CDS             complement(20622..21707)
                     /gene="xerS"
                     /locus_tag="ACHBFW_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFM9317533.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT         7448 a         3297 c         4221 g         6938 t
ORIGIN      
        1 atttttaaga agggaaacaa tagtgagcta taatgtacaa ccgttaagga caaatgaaga
       61 gatacaagat tttcagtttt ggctaagaag aacaagtaat ccggaaagag atagtttttt
      121 atttttgttt ggaatcaata atgggttacg tatgtcagat attataggat taaaagttgg
      181 agatatacga ggcaaaagta agcctattat tgttgaacat aaaacaggta agcgaaaacc
      241 gatttttata gataatttga gagaagaaat tttattatat actgaaggta aagaagaaaa
      301 tgattggtta tttccgagtc ggcaacaagg tcgacatata acaagggatc gtgtctatca
      361 gatatatgca gatatagcag aaaaattagg acgagatgat ataggaacac atacattaag
      421 aaaaactttt gggtatcact actacaagaa aactagagac attgctacct taatgtttat
      481 ttttaatcat agctcgcaag caattacaaa aaggtacatt ggtataactg aagatgaaat
      541 aggagcatca cttcgtggat ttaaactagg tgtataattt aaaaattttt ataaacagta
      601 aagacaaaga ttaaaagcta tttttatcta gaacgatttt atttcataaa gttcgcggaa
      661 taaaagctgg ggttactggt acttggtata ggttcgtgtt atataaacta agtaattaaa
      721 taacattttt aggttggaaa aggatgaaat taaaaaaaga gaatcaatac ggtctcaaaa
      781 acgcaaacgc tgcggaataa gaaaaatact gataactcaa cgtttagcag actatctaat
      841 agggatagtc tgcttttttc gtttttgacc atacttttga tcttctgcaa acttccttgc
      901 ttttcctctg tagaactcat gaaatctgca aaacgaattg ccgtttcctc tgtctgcttt
      961 ttcgcgatat gggtgtatat atcatccata gtggttttaa tatctgtatg tccaagtcga
     1021 acatacactt ctttgacaga cgcaccagat tcaaagagca gactacaatg ggtatgacga
     1081 aaaccatgaa gtgtgatatg ttttaagtta ttctctttaa tgatgatttt caaattgtga
     1141 tttacatagt ctaaataaag gggcttattt tcttctgatg aaaacaaaag ttgttcacca
     1201 tcttttatag gtttgattct catttttaga tattatgccc gcacgtattc atcacatttc
     1261 tattgtgaat cgctttattc gaccgacatt tgacttttat cataatatac tcggtttaaa
     1321 attactaatg aaaacaatta atcaagacga tcataccatg tatcacctgt ttttttcaga
     1381 taatcaccac cgtgtgggaa ctgaactgac tttttttgaa gtacaagaag ggaacaacca
     1441 gttttttgga accaatacga ttgaacgaac gatactaaaa gttccttccg aagcatcatt
     1501 gcatttttgg gaaatatatt ttgaaacaca aggcgtttgc cattatggaa tcgaatcatt
     1561 ttctggacga cctatcttac gttttgaagg gccagatgca acacgattag ctttagttcc
     1621 cttacgcgaa tttgaagata ttgatgatta tttcccgtat gtcacagacc agatccctac
     1681 agagcatgcc attttaggaa ttgatgccat tcaactacgc gtacaatatt ccgatgcgac
     1741 tgaacgagag ttattgtcgg taggctggta gcacaaagaa tacgtcagtt tcttttcggg
     1801 tcatcaacga gtgacaatct tagaaaatca agacactcag ttctatcaag aagttcagat
     1861 tattcaagat cgtgagaatc cagttgccat tgaagggatt ggtagcgtcc atcatgttgc
     1921 gtttggcgta gaaaataaat ctgatttaca acgcatcgac aaacaattac aagagcgcaa
     1981 ttttataaat tcaggaataa aagatcgtga attttttgta tcactttatt atcgtgaccc
     2041 aaatcaatta ttgattgaaa ttgctacagg ggaaggaaac ctagatgcaa aagcgtatga
     2101 aaatcaaagt ccacgctttg aagaaattcc tttattttta ccgcagtact taaatttcat
     2161 tcgtgaacaa gtggagcaat aattagctaa tcaaatccat gacgaagagt aagaggttga
     2221 ctcataagtc aaaaaataaa gaaaaacgca tcaaatccat tctaaagaat gttgatttga
     2281 tgcggttcct atttttataa actatcattt tcgactaata aaactactta tgagtcagcc
     2341 tctctttttt tcttgatttt tatacctctt tctaggtaaa ctaaacatct gccaaaaata
     2401 ccgtgtctca ttgaaacatt ctcaactatt tcaaaataaa ggggagtacc aaatgaacga
     2461 gtatattgaa attatccacg caagagaaac aacttaaaaa tgtcagtctc aaaattccca
     2521 aaaataaaat tagtattttt accagcgtat ctggttcagg aaaatcatcg attgtttttg
     2581 atactattac ccaagaagct ggtcgtcaac tgaatgacac ctatgatagt tttacccgtt
     2641 tatttttacc taaatacagt cgtccagagg ttgatgagat tgataatctt tctacggcga
     2701 ttgtggtcaa tcaaaagaag attggtggca gtgcgcgctc aacgttaggg acgattacta
     2761 acatcaatgc gttatttcgt gtgttgtttt catgttttgc aacacctagt ttaggatatg
     2821 caaatgctta ttcatttaat gatcctagtg gaatgtgttc tgtctgtcag ggaatcggta
     2881 agacgattgc atttttaata ggattgcttt atttttcggc tattgataat gctgattttg
     2941 tattttctga tccatttacc gcagaagttt tgtctggaat tgaggaacag gcaaagaatc
     3001 atggttattt tgtattggtg cataatgtta attcagcaga agatgttaaa actattcaga
     3061 gaaattggcg ttttgagggg ttcattgttg ttggtgtacg tgtaaaagaa tttttggaat
     3121 taaataacgt ttttgatggt cctgttgttt atattgatac gcatctttca aaagagacat
     3181 taagagaact tgaacaacaa atgaatcgtt tatttgtaaa tacaaatgat taccaagcta
     3241 gtacattggc agtggaacat ttgattcatt atggtcatga gaaaatagcg tttctttctt
     3301 ttgactacat tgtaggtgaa ccaagtgtta ttcaagaacg acatcaagct tatatcgata
     3361 cattaaagaa atatcattta acctttaaat cgcattggat atataacaac tctgaatttg
     3421 agcgaattta tcaagaatta gctgaattta ctgcaattgt tgttactgga gattatttag
     3481 cggctaaatt agtaaaattt ttaaaaaata agaataaacg agtgatggaa caattatcga
     3541 ttattggatt tgatgatatt acttttgcag aattaatgga tcctgcgttg acaacaatac
     3601 gactaaatcc aggtaacaag ggggcgattg cttttcacca actgattgga ctggccaaac
     3661 aagaagaaat aaaaaatcaa gtactactat tagagggaga attgatttct cgagactctg
     3721 ttcacaaaat caaataaaaa agctctgtga aataagtata gttaacaata aaatatccga
     3781 acgatagaga gaatgaactg tgatctagca gttcctcgtc gcaaattcac tagaattagc
     3841 attatttact ctaaattgtt cggatatttt ttcttgtttt taattatgat tacagtgcct
     3901 atttttaacg aaatttgctt attttcaaaa tgtatgatta tatgttagta aacactttca
     3961 aatatgaaaa gaggagctat tatggtagga attagggatg ttgcaaaacg ggcgggtgtg
     4021 tccattgcaa cagtatcgcg cgtgctaaat gaggatccga cactttcagt aaccgatgaa
     4081 accaaacgaa agattgcaga atcggttaat ttttatcatt atgtcaaaaa aagtcccgtt
     4141 gcaaaaaatt taacaaatat tgcattaata acgacagttt ctgaagtaga tgagttggaa
     4201 gatccctatt ttcgaacgat tcgcagaggg attcaagtcg aagcagaaca aagcaaagtc
     4261 aatttgaaga aaattattcg tttgtcagaa acaccgttgg agcaagacga tttgctaaaa
     4321 tgtcaaggtg tgttgattat tgggcaggtg ctatcttctg tgattgaaga agtatcagca
     4381 attaattcga atgtcgtagt gattgatgac cctagtattg aagctgaagt agatgcagtt
     4441 tatactgatt taacaaaagc aacgaatcag catttagatc gattgtataa aaaaggacac
     4501 cgaaacattg cgtttatcgg aggaggacgt gttcgtttag atagccaagg aaataaacac
     4561 gtgtcagatg atgattatcg aaaaatagcc tatgagacgt ggatgaaaca aaaaggatta
     4621 gaaaaatata gtcaaattta tttagaaggc tggaccacac ttgacggaat gcaggcagca
     4681 gatttacttt tgaaaaaaca tgctaaagat aagttgccga ctgctatcat ggtaggaaat
     4741 gatcctattg ctgtgggggt ttatcgttca cttcaaaaaa atggtctaca tattccagaa
     4801 gatatttcga ttgtgagttt tgataatatt gaagttgctg aatttttaac acctgcatta
     4861 aatacggtga atgtgaatac agaagaaatt ggaaaactag cagttcgcat ggttaatgaa
     4921 cgaataaaag aagttagaaa tgtttcgatc aaagtgattg tttctaataa tattattgtt
     4981 agagaaagtg aaaaaaatat cgattaaaaa agtaaacatc caaaaaaggg tgtttacttt
     5041 tttgtttact aatattttta gtaaaaaatc tcgacaaaac attaagtaaa cagttatggt
     5101 gttgttaaca aaaagaaaac gcattcaaaa caggaggaat taagatgaaa catttcaaaa
     5161 aaatagcttt aacagggata gctgttttaa ctgggacatt actcgcagca tgtggtgggg
     5221 gagatagtaa ttctaaagac gggcaagtaa ctttaagcta tgcgagttga gatactgacc
     5281 aagctccagg actaagaaaa atgttagatg aatttgaaaa acaaaatcct gatatcaaag
     5341 tagaaatgga gacaacgcct tgggatcaat attgggttaa attagaagct gcaacaacgg
     5401 gaggtaatat gccagatgta gtaaccatgc actctagcga atcctataaa tatatgtcac
     5461 agaatgcttt gatgggactt gatgatgtta tcaaagaaaa tgatattgac atgggaaact
     5521 ttactgaagg aattgctgat ttctatacgc ttgaagacga actatacgca atccctaaag
     5581 atgcttctgt agtggggcta tggtacaaca aagaattatt tgataatgca ggtgtcgatt
     5641 atccagatga aacatggaca tgggaaacac tcagacaagc tgcaattgat ttaacagatg
     5701 aatcaaaagg catctatggt tttgcatcac atattggaac agagactggc tactggccat
     5761 ttatttatca aaatggggga gaagtatttg ccgatgacaa tacaaaatca ggtttgaata
     5821 ccccagaagc acaagaagct ttagcttttt atcgagatat tgcacttgtt gatcatgctt
     5881 caccaacaat tacagaatta caagatcctg ataagttaag tcgttttcaa tcaggtagat
     5941 tagcaatgat gattgaagga aactggtttg caccgggatt tttgaaaaat gactatctag
     6001 ttgaaaatgt tgcttctgcc gtgttaccta agggaaaagt taatgcaaca attacaaatg
     6061 gacttggttg ggctgccagt gcgagtacaa aatacccaga agaagtgaaa aaactaatgg
     6121 catttttagc tagtgaggac gccaataaaa tccaagcgga aacgggggca tcaatcccag
     6181 cactaaaagg ctttgaagac gagtgggctg cttcttcaga tacaattgat ttgagtgctt
     6241 ttggaaaaat gttggagtat ggagtccctc gtccatacaa taaagatgga ttacgagctg
     6301 aacaagcaga agttcaaatt attaaccgta ttctgtctgg tgaagtaact gttgaagatg
     6361 gaaccgctca aattgaaaaa gaagtcaatg cgatattgag cggaaattaa attagttaat
     6421 tatacaatat acaggcgaaa ggattctatt tcgtctgtat attaatggat gaataccaaa
     6481 aagggggata ttatgaaaaa tcgaattttt ggaaaatatc gtaaagatca agtgatatgg
     6541 ggctatattt ttatttttcc aacggcatta ggattaattt tattaaatat atggccagca
     6601 attcaaacag caattttaag ttttcaaaaa acggtagggt tcggtgatac tgaatgggtc
     6661 ggattaagaa attaccaaaa aatgtttgca gacggtgaag tttttcagtc tttattaaac
     6721 acattaattt atgcagtggt ttcgatacct agtattgttg tattatcctt gttggtggca
     6781 gttttaatga atagaaaagt taaaggattg tcgatttatc ggacaattta ttttctacca
     6841 gtcgttgctg caccatccgc agtcgcaatg atttggcgct ggatgttcaa taatgattat
     6901 ggactgatta ataacatgtt ggctaaaata ggtctcgatg gaccagcatg gttaacggat
     6961 ccatcgattg cgatttattc tattatcatt gtaggagttt ggagtgctat tggttataac
     7021 atggtgttat tactagccgg cttataagaa attcctaaag attattatga agcagcggaa
     7081 attgatggtg ctagtccaat ccagcagttt ttcaacatta cgttaccatt agttacacca
     7141 acattatttt ttgtagttgt aacaacagtt atcaatgctt tccaagtatt tgatgttatt
     7201 tttatgatga ttactcctaa tagtaccgcc atgtttaaat cacaatcatt agcctattta
     7261 ttctataaac attcatttat tttaaatgac aagggatatg gttcggcaat cgttatgttc
     7321 cttttagtga ttatattaat tattactgca attcaaatga agttgcagaa aaagtgggta
     7381 aactattaag gaggctatca aattatgaaa aataaatttt tttcaaatgc gccaatccat
     7441 atcatgctta ttttaggtgg tattgcaatg gcattacctt ttatttggat gattttaact
     7501 tcagggaaaa cgttggttga atctacccaa attccaccta caattttccc tgagaaattt
     7561 cagctaagta actatcaaca agtacttgat ttattaccat tcatggcttt ttatatcaat
     7621 acatttttaa tggttgttgg acgagtgatt ggctcggttt tattttcagc aatggctgca
     7681 tatgcttgtg cgcgtctaag ttttccaggg aaaaatatcc ttttcagctt agtacttatt
     7741 caaatgatga ttcccggttc catttttatt actccacagt atttattagc acaaaaattg
     7801 ggtattttaa atacaattcc agcattaata ttaccagggg tagttagtgc gtttggtacg
     7861 tttttattaa gacaattttt cttaggttta cctgatgaat tggaggaagc ggcctattta
     7921 gaaggtgcca atatttggac gatctttttc aaaattatgt tgccgcttgc taaatcagga
     7981 ttggttgcac ttggaatatt cacagcgttg tttgcattta aagatttaat gtggccattg
     8041 attgtcaata cttcaattga taagatgcct ttagctccgg gattagcaaa cttacaagga
     8101 caatatgcca cgaacttccc acaattaatg gcaggttcgt tattagcaat ttggccaata
     8161 gttgtgatat tcatcgtttt ccaaagaaaa ttcattgaag gaattgctac aacaggtagt
     8221 aaattataaa actattaatt agtgattgga gagtaagtac atgacaaaaa ttacatttat
     8281 tggtgcagga agtactattt ttgcaaaaaa cgtattaggc gatgcgatgt taactccaag
     8341 tctacaagat gcacatattg cattgtacga tattgatgaa aaacgactaa aagaatcaaa
     8401 tttaatgctt aaaaatatta ataaaaattc gaatgaaaat cgtgctgata ttgtttctta
     8461 ttatgatcgc aaagaagcgt taagagaggc tgattttgtt atcaatgcta ttcaagtggg
     8521 tggttataaa ccttcaacag tgattgattt tgatattcct aaaaaatatg ggttgcgaca
     8581 aactattggt gacaccatgg ggattggtgg gatttttaga accctacgga cactaccagt
     8641 aatgttagac tttgctaaag aaatggaaga agtttgtcca aatgcttggt tattgaacta
     8701 tacaaatccg atggcgatgt taaccatggg tgttttaaaa gcgacgagta ttaagactgt
     8761 tggtttatgt catagcgttc aagtatgcgt accagaattg tttgagcatt taggaatagc
     8821 cgatcaatat gacttaaatg actttcaatg gaaaattgca ggcattaatc atatggcatt
     8881 cttgttggaa attacaaaaa atggggaaga tttctatcca acaattcgtc agttagctgc
     8941 agaaaaacca aatccacatc cagattctgt acgttttgaa ttgttgaaac gttttggtta
     9001 ctatattaca gaatcaagtg aacacaatgc agaataccat ccatacttta ttaaatcaaa
     9061 atacccagaa ttgattgacg aattagggat tccgttggat gaatacttac gtcgttgtga
     9121 acaacaaatt aatgattggg aaagcatgcg agaggacatt gtaaataatg gggagttaac
     9181 acatacgaga agccgtgaat atgcttcata tatcatggat tctattacga caggaactcc
     9241 gtcagtgatt gcaggaaatg tcttaaataa aggcttaatc acaaacttac cgacagatgt
     9301 ttgtgtggaa gtaccttgtt tagtcgataa aaatggcgtg caaccaacct atgtcggtga
     9361 attgccaaca caattagctg ctttaaatcg gacgaatatc aatgttcaag aactgacagt
     9421 agaagccgct ttaacactag ataaatcaaa aatttatcaa gcagcgtatt tagatccaca
     9481 tctatcatct gaattgtctt taacagatat caaaaattta gtggatgatt taatcgaagc
     9541 acatggggac tatttacctg aatataatta aaaagaagct gaaagagtaa ggaattttca
     9601 attccttact cttttcttag ttaaatttag taaaaatatt gacaaaacac ttagtaaaca
     9661 gttacattag tgttaacaaa aagaaaacgc attcaaattg tataaggagg agcataaaat
     9721 gaagtggtct aaaaaaatta ttttagcttg tatagcacta ggagcaacga tctctttgac
     9781 tgcttgtgga aaaaatcagg aagatgcaag cggtaaggta gaaatagagt tttttaatca
     9841 aaaaaaagaa atgactcaaa cgattcagga gattgctaaa gattttgaag caaaaaaccc
     9901 tgatattcat gtaaaagttg tggatgtacc gaatgctggt gaagtaatta aaacacgtat
     9961 gcttgcaggc gatgtaccag atgtcattaa tctttatcct caaagtatag aattgaaaga
    10021 atgggcgaaa gctggttatt tagaggactt aaccgaagaa ccgtatttag agaatatcaa
    10081 aaatggatat gcacaacgtt ttgctattga ggatcgtgta tatagcatac cattaaccgc
    10141 taatgtctat ggtttttact ataacaaaac tgcttttgag gaaatgggga ttcaagcacc
    10201 agaaacttgg gcagaatttg aaaaaatagt agctgagatt aaagatcaaa acaaagtacc
    10261 gtttgcgatt gcaggagcag aaggctggac tttaaatggt tatcaccaac tagctatagc
    10321 gactgctgct ggtggtgaag aggaagcgaa caatgtttgg cgtttctcag aagttaatgg
    10381 aattaatgct gaaagcaaag aaatgcaaaa agattttaat cgattagatt tgttaagaga
    10441 accaaaagct ttgcaaaaca actggcaagg tgccggttat aacgatacag tagttacttt
    10501 tacaaaaggt gatgcgttga ttatgccgaa tggttcatgg gcaatgccga tgattcattc
    10561 acaaaatcca gattttgaag taggaacttt tccttttcca gcagatgaag caggtcaaag
    10621 tctaacgatt ggcgcaggag acctagcctt atcagtttct gcaacttctc aacataaaga
    10681 agaagccaaa cgttttgtag aatatatgac aacacctgaa gcgatgcaga aatattatga
    10741 tgtagatggt gcaccgtgtg ctgttgaggg cgttatcgag aatactcagg attcaccatt
    10801 aagtggttta actgaacttg cttttactga tcgtcattta gtttggttag ccaaagattg
    10861 gaatagtgaa aatgattttt ataccttaac aacaaattat ttacacaatg gcaatcaaaa
    10921 agccatgatt aacgcattaa atgctttctt taatccaatg aaagctgacg gaaggtagga
    10981 aaataaaatg aaaataaaag aaattttaaa tcgttactgg gcaatcattt ttgtagcaat
    11041 tccaattatt ttgcagatga tttttttcta ctttccaatg gttcaaggtg ctttttacag
    11101 tttaactaat tggactggtc tgacttataa ttttgatttt gtgggtttga acaattataa
    11161 gattcttttt tctgatccga agtttatcaa atcgattggc ttcactttaa ttgtgacgct
    11221 ttgcatgatt gctggacaaa ttggttttgg tattttgatt gcgcgggcgt tgaacgcaaa
    11281 tattaaaggg aaaacattct ttagagcatg gttctttttt ccagcggtct tatcgggcgt
    11341 ggttgtttct ttagtattta aacaaatttt taactatggt ttaccaaaaa ttggtagtat
    11401 gttaggcatt gaatttttac aaaccagtct tttaggaaca acatggggag cgatttttgc
    11461 agtaattttt gttttacttt ggcaaggaat tgccatgcca gtcatcattt tcttagcggg
    11521 ttttcaaagt attccacaag aaattattga agctgctaga atggatgggg cgaatgataa
    11581 gcaaattttt tggaatattg aaatcaccta cttattacca agtatttcaa tggtatttat
    11641 tatggcatta aaaggtggct taacagcatt tgatcaaatt tttgctttaa ctggtggtgg
    11701 accaagcgat gccacaacat ctttggggtt acttgtttat aattatgctt ttaaaaacaa
    11761 tcagtttggt tatgcgaatg ctattgcttt agtcttattt atcttaattg ggattgtttc
    11821 actggcacaa ttaaaattat cgaaaaaatt tgaaatctag gaggtttccc aatgaaaaca
    11881 tcaaaagaaa aaaatatttc taaatacttg cttttattat tagggggctt catgatttta
    11941 gttcccttag tttcaacgct attcggttca tttaaaacaa cacccgatat tatgaataat
    12001 ttctttcgtt ttcctaatcc agcgactttg gaaaatttcc gaagattgtt agcggatgga
    12061 attggtcatt acttttggaa ttcggcaatc atcacaatca ttgcagtagt tgctgtgaca
    12121 gcaattattc ctatggctgc ttattcacta gcaagaaaaa tgactcgtgg gaaagcatac
    12181 ggattgatgt atttattttt aattttagga atttttgttc ctttccaagt aattatgatt
    12241 ccgattaccg ttatgatgag taaaattggt ttagcaaata tgtggggatt aatcttactc
    12301 tatctcactt atgccgtacc acagacacta tttttatatg ttggttttat taaactaaat
    12361 gtgcctatca gtctggatga ggctgctgaa attgatgggg ctagtcactt tacaacttat
    12421 ttcaaaattg ttttcccaat gttaaaacca atgcatgcga cgactattat tcttaatgct
    12481 ctatggtttt ggaatgattt catgctacca ttattaattt taaatcgtga ttctaatatg
    12541 tggacactac cattgtttca atataattat acgggacaat attttaacga ctatggtcca
    12601 agttttgctt cctatacagt aggaattgtc acgattacac tagtttatct tgctttccaa
    12661 agaagtatta tagctggaat gagcaatgga gcagtgaaat aaccaataaa ttattctcat
    12721 tataaaggag ttcataaact tatgattata tttgacaatc acacacaaac atttcattta
    12781 caaaataaaa aaattagcta tctattagga atcgaagaaa ataattattt aacgcatctt
    12841 tattttggaa aaaagattac gcactattct ggtgggtatc gctacccccg aacagatcgt
    12901 tccttctcac ccaacaatgc aaatgcgcaa gatcgtttat tatctttaga cacattacca
    12961 ttagaatttc caggatatgg acatggtgat tttagagaac cggcgcataa tattaaatta
    13021 ggaaacggta gtcgtgtaaa tgattttcgt tatgatagct acgaaattca aaaaggaaag
    13081 cccagcttag cgggattgcc agccttatat acggaagatg atgatgaagc tgaaacgctg
    13141 attattcaat tgaaagacgc gattagtgga ttgactttac agttgttcta cacaatttat
    13201 caaaatcaca gtgtaattat tcgttctacg caattagtaa ataatagtga tgaaacagtg
    13261 gagattaacc gcttagctag tcaatcaatt gattttccaa atcgtccact cgagttaatt
    13321 catttaaatg gtacctgggc aagagaacgt caaatgacta gagaaaaaat tcatacaggg
    13381 ataaaagtat tagatagtaa acgcggatca agcagccatc aacaaaatcc gtttgttgct
    13441 ttagtagatg ccaaaacaga tgaatttcaa ggagaggcat atggatttaa tttagtttat
    13501 agcggtaatc acgaaacggt gattcaaaaa gatccatttg accaaacacg ggtcattatg
    13561 ggaattaatt catttaattt tggttggcaa ctaaaaactg gcgactcatt tcaaacgcca
    13621 gaagtcgtga tggtttatag taatgaaggt ctgaatacgt tatcacagac gtatcatgaa
    13681 ttgtataatc accgtttaat tcgtggaaac tatcgtttaa aagaacgtcc cacgttaatc
    13741 aacaactggg aagcaacgta ttttgatttt aacgaagaaa aaatcatgaa tattgtcgat
    13801 gatgcgaaga acttgggcat tgaaatgttt gttttagatg atggttggtt tggcggtcgt
    13861 gaaaatgact tcacttcatt aggtgattgg gttgaaacag aaggcaaact tgcttgtggt
    13921 ttggaaaatt tagccaagca agtgcatgat aacgggatga aatttgggat ttggtttgaa
    13981 ccggaaatga tttctgaaga tagtgaacta tttcgagcac atccagattg ggcattgggt
    14041 attccaggtc gtgaccgctc aattagtcga aatcaatatg ttcttgattt ttcaagacaa
    14101 gatgttcgtg ataatatcta tcaacaaatg acggcgattt ttgatcgtgt accgattgat
    14161 tatgtgaagt gggatatgaa tcgaaatatg acagaagtct attctgtctt gttggactca
    14221 acgatgcaag gtgaagtggc tcatcgttat atgttagggt tatatgaatt tttagaaaag
    14281 ttaacaacga attatccaac tattttattt gaaagttgtt cgggtggagg cggtcgtttt
    14341 gatgcaggtt tcttgtatta catgccacaa acttggacga gcgacaatac cgatgccgtg
    14401 gctcgtctga aaattcaata tggaaccagc ttagtgtatc caatttcaag tatgggtgcg
    14461 catgtttcag cagtcccaaa tcatcaaaca ggaagagaaa cgagtttaac tattcgtggc
    14521 aatgctgcga tggctggtgt ttttggttat gagctagatc taacgcaatt aacttctgaa
    14581 gaaaaagcag aaattaaaga acaagttgcc ttttataaaa agcatcgcca acttttgcaa
    14641 tatggaactt tttatcgctt ggaaagtccg tttgaaggca atgatactgc atggatgttt
    14701 gtctcaaaag accaaacaga agccatggtc ttttatttcc gagtattagc agaagctgcg
    14761 catccattag tcacattgaa attagcagga ttagcagaag aagcaactta tcaaatggaa
    14821 gatgtaacat ttgctggcga tgaattgatg aatctcggtt tttatattga ctcagaacta
    14881 catggtgatt atgcgacaca acgcttttat ttgaaacaga tatagtagct tgaatttaca
    14941 atataagtgc aacaccaaaa gagactcatt ccaaacgaag aaaatatgtt ttatttacac
    15001 gcatttacta aaaagcagcc tgatttgaat tgggaaaatg aagaagtacg tgaagaaatc
    15061 tatgcaatga ttaactattg gttagataaa ggactaggcg gatttagaat cgatgcgata
    15121 ttaaacatca aaaaaaaact agagtatggc atctttgaat ctgatggaga agatgggtta
    15181 gccttcattg ggccctggat tcttaatcag tcgggcattg aagtgtggtt aaaagaaatg
    15241 agagagcgga catttaaacc acataatagt atgacggtag cagaagccga tgttcctaat
    15301 gaacgtttag atgaatatat tggtgaagat ggctattatt cgatggtttt tgattttagt
    15361 tatacggata ttgatgtacc tgaaactggt gaatggttta aagatagcca atggacatgg
    15421 acggatatgc gtaccaatat ttttacgaat caattagtta cgcaagataa aggttggggc
    15481 gccttatatt tagaaaacca cgatcaacca cgttcaatta ataaatatat tccagaagaa
    15541 tggatcaatg attatagtaa aaaaatgttg gcgacgcttt ttatgttact aagaggtacc
    15601 ccttttattt atcaaggaca agaactagga atgaccaaca tagaaatggc atcattagaa
    15661 gattttgatg atgtagctac gcattctcag tataaacggg cattagaata tggattggca
    15721 ccagagcaag cattgaaagc tgtcaataaa cgaagtcggg ataattctcg cacacctatg
    15781 caatggaata cgcaaaaaaa tgccagattc tcaacttctg ataatgtatg gttaaaagta
    15841 aatcctaatt atccagaact caatgcagaa gctcagttaa ccaatcaaca gtcggtattc
    15901 aattattatc atcaattaat taaattaaga aaaaatagtg tatacaaaga cgtgctaatc
    15961 tacggacaat ttctaccagt gaaagataaa aatgaacata ttttgcttta tgaacgccaa
    16021 ttagaagata aaatcgtatt agttatctta aacgttacag caacagaaca agaatattat
    16081 gtagatgaac agtaccaaca agtattaatc aataattatg aagatgtttt attagatcaa
    16141 agtcgaaaat tgcgtttacg accgtttgaa agtattgtat tagctaatta tggaggacaa
    16201 ttatgaaaat taaaaacgaa gcaatgctca tcacctatgc agatagttta ggaaaaaaca
    16261 tgggcgaatt aaaacaagtc ttagacaaac atttacaagg tgttattggt ggtgtacatc
    16321 tgttgccttt ttttccatcg acaggtgatc gaggctttgc gccgagtgat tatacgacag
    16381 ttgatccagc tcttggaaca tgggaagatg tggaagcatt aggtgaagat tattatttga
    16441 tgtttgattt tatgattaat cacatttctc gtgaatcaaa atttttccaa gatttcaaaa
    16501 aaaaccatga gcagtcacca tacaaagaaa tgtttatccg tattcatgaa tttttcccag
    16561 aaaatcgacc aacacaagaa gatattgatt taatctacaa gcgtaaagac aaagcgccgt
    16621 ttcaaacggt agaatttgcg gatggtacga cagaagaggt ttggaatacc tttggggaag
    16681 aacaaattga tttagatgtg accaaagaag ttgtgaaaga atttattcga gaaaccatca
    16741 aagatatggc gaaccatggc tgttctttaa ttcgattaga tgcgtttgct tatgcgatta
    16801 aaaaattaga taccaatgat ttcttcgtgg aaccagatat ttgggaactt ttagacgaag
    16861 tgcgtcaaga agcagcgaaa tatgacgtcg aattattacc agaaatccat gagcattatt
    16921 ccattcaaat gaaaattgcc aatcatgatt attatatcta tgacttcgcc ttgccaatgg
    16981 tcactctgta ttcactttat agtggaaaat cagaacgttt agccaattgg ttgaaaatga
    17041 gtccgatgaa gcagtttaca acattggata cgcatgatgg aattggtgtt gtggacgctc
    17101 gtgatttatt aacagatgaa gaattggatt acacctctga ggaattgtat aaagtcgggg
    17161 cgaatgtgaa gaaggtctat tcaagtgcca attacaataa tttagatatt tatcaaatta
    17221 acagcaccta ttatagtgca ttaggcgata atgaccgtag ctatctgtta gcacgtgcga
    17281 ttcaagtatt tgcgccaggg attccacaaa tttattacgt tgggttatta gcaggaaaaa
    17341 atgatattga actgttggaa gcaacgaaag aagggcgtaa tattaatcgt cattactatg
    17401 atttagccga aattgaagaa gaagtgcaac ggccagtgat tcaacaatta tttgcacttt
    17461 taaaatttag aaatgaatct gcggcgtttg atttggatgg aacgattgaa gtagcaacgc
    17521 catcagaatc agaaatagtg attacacgta aaaataaaaa cggtagtgat agtgcaacat
    17581 taacggctaa cttggatacg aaagaattta tcattgagtc taaagatata aaaattttca
    17641 ctagtgttgc attaaaattt taacctattc catagaacaa aaaatccttt atactagcac
    17701 ttggatgact tatccaagac caataaaaag gactcataca tggataagta taaaacaaat
    17761 acagcattta ataaatggtt ttctgccata aaattaggaa aactttctgt caactcccaa
    17821 aaatgtattt ttgattacaa ttcgtatcgt aaaaaactgt cttttgaagc tgtgctcaag
    17881 ctctttctct acgcagttaa tgaagaaaaa gaaagtttgc gtgatttgag tacagctctt
    17941 atcgaccgat cgctccaact agaaaccgat gtgtcatcga ttagtcatac ccaattgtct
    18001 cgagcattta atgcacttga acctaaagtg ttagaagaaa tttttcaaca actgttagaa
    18061 aaagttcaac atcaaatcaa accaaccaag cgaaatcatc tgtatctgat tgattccagt
    18121 acgttttcgc ttagtttaaa acgtcataag tgggcgactt tccgtcaaac aaaatcaggt
    18181 gtcaagttac acttaaacta ttgttatatg gacgatacta cgatgtatcc aactgatttt
    18241 accttaacaa atgcccatga acatgatctc aatcaactac cagtattggt gaatcaacca
    18301 gaggcaactt acgtgtttga tcgtgggtat taggactttg aaaagatgga tcagatgcat
    18361 tgggatggct atttcttcgt cactcggatt aaaaaaaaca ccaaagttca cgtgatagat
    18421 actctggaaa catcgccaga aacagaaatt ctacgagacg agctggttcg tttaggatct
    18481 aaaacctatc taaccgccaa tttccgacta gtcactgttc aagataagaa cggacgtgtg
    18541 tttcaattta tcaccaatcg aatggacgtc tcttcaaaag aaatctctga tatgtatcac
    18601 gctcgttggc aaatcgaatt gtttttcaag cacatcaaac aacacatgac cattaaaacc
    18661 ttcttttctc aaagtgaaaa aggcgtgcag aatcaattga ttttaacgat gatttccgct
    18721 ctcctcacgt ttctcatcaa attggagacg aaaacggaaa aatcagtgtt ccaaatcaaa
    18781 cgattctttc gttatttact gtttcagccc tttgaatgtt gtctagaaaa attaattccc
    18841 acatgagaaa aaagaaattt ggctcgttac ttttggtttt cagttgaata acagataaaa
    18901 aattttctgg aaaaagtcat cctctgcgtc agcacagttt tttttgactt attttcagag
    18961 gaaatagttt tcagaatatt ctaaaatgtt ttatgcaaca ctagtgatat tttttagaat
    19021 tagatttcta atgtgcattt tttgagtatt aaggagcaag tatacaaaga tttataaagc
    19081 tgattaaatt tgaataaaaa tatgcaggaa cgctactatt tttagtagtg ttctttttta
    19141 tatttgaata aaaaatgtgg tggttaaatg gtggttataa caaaaaatga attaatgaaa
    19201 ttaggttatg ggaagtatca gtctgagaga atcatacgac aggcaaaagc aattatggct
    19261 tcaaaaagct atacctatta cgaaaataga agattaggac tagtacctgt tgaatctgta
    19321 gaagaagttt taggtatgaa gatagaggta gaggaggatt ttcagaatca tggttaattt
    19381 taaaaaagat aaaaatggga aatatcattt tactgctagt ttgggattcg acaaacgaac
    19441 agggaaaaga gtacaaaaaa acgtagaggg gttaaaacag ttaaagaaga aaaaaagcgt
    19501 ataccgaact tgtttctaat tatggaaaag aagcagaaac ttctcaaagt actatgttgt
    19561 ttgaggagtt tttttataaa ttattcctac cttattatca agggaaagta gaagaaagaa
    19621 catataaaaa tcggttatct acaatcaaaa aaacttttct tatttcttta aaatggagtt
    19681 aagagaaata cgtccaattg acattcaaaa gtggcaaaat aatttattga gcaaatataa
    19741 aaattcttat gtaagaaaca tgtatgggat ttttcagatg tcattagatc gagcagttgt
    19801 tttgggacta cttgaaaaaa atcctgcaaa agttattgga aatgtgaaga aaaggaaaac
    19861 tacagttgat ttttggacaa aagaagaatt tacaaaagtt atttcaactt tttatattga
    19921 tgatttgtac caacattttg gttttatttg tatgtggtta ctttttatga ccgttatgcg
    19981 tttgggagaa gccactgcat taacttggaa agatattgat tttgaaaata aaaacttgcg
    20041 tgtgaataaa acactctatt ataagagagc caaaacctat gagtttgtag aacccgaaac
    20101 ggcttctagt attcgtacaa tcgaactaga cgacaataca attacctttt taaaagcttg
    20161 gaaggagcgt caagtttcaa taggaggcat gtcgtttgta ctttcttata atggaacacc
    20221 tactcagaaa tatacaatcc gtcatattat gagcagacat gcaacgatcg ttggtgttca
    20281 tataatccgc atacacggat taaggcatag tcatgcttct ttgcttatat cacaataaaa
    20341 agatgcactt cgaattaaag aaaggttaaa acatgctgat gtacaaacga ctttaggaac
    20401 ttatggtcat ttgtatctaa attcattgag agagataaca aatgatccca cagattatat
    20461 ccaaactaat ttttctaaaa tcaaaccgac acctatttac gataataaaa aataaatgat
    20521 aaacaaaagt aggatcacac caaaagaaac acactagaaa aactataata caggcttttc
    20581 tagcgtgttg tttcttattt ccgcctattt atacattata ctcataaagc atttaatgca
    20641 tcgatcgtag ccgcattgtc tatatgggta tataaagttg ttgtattggt acttgaatgt
    20701 cccaattgtt gggcagttaa taccaatgaa tttgtctgtt ggtagagtct agtagctaat
    20761 gtatgacgca gtttgtgagg gctgacttga attctaaagc cttttgaata ttttgctacc
    20821 atccgttcga ttgcaatacc ggatatacgc ttggcttctc cttggtaaat tgctagaaat
    20881 aaagcttttt cgttttctgt tactttatat cgttctttcc gaatagctag ataattagct
    20941 aaatattcca ttgcgaatgc tgctatattg acagcatctc ttttatttcc ttttctagtt
    21001 actaccacat tattcttatc taaagatagg tcatctaaat taatatttat aacctcggag
    21061 agtctcaatc cagatcctaa aatcagtgca ttgatcgcca ggtctctttc tacatctcgt
    21121 tgataataga tcaaagctcg tttactcaaa gtatttttat attcattggc aatataatct
    21181 agataatttt gtgtatcctt atctagaaat aattttttct caatggcatt ggcacgtgca
    21241 gctaacgtag ttttatctgt gatggttgct acttgatcca taggattatt gtctaagtat
    21301 ggttttccta gaggattatt ggaacttttc gaaagatatt taaatagaac ctttaatgcc
    21361 gtgatatttc tattgatagt agaagtagat agacctaagt tattattatc tttgagctta
    21421 gtttttgttg tctctttttt tacattttct tttgtgcgac gtttcaaatg agatttgtac
    21481 aagtttaggt cataggcagt taactgtctt atcgaggtga tagaagtatc aggaacgata
    21541 gactcactca gaagccattc ataaaagagc ttataatttt ttagatattc taaagtggtt
    21601 cgaggtgatt gatctttatc ctcacgatct tgaacaaaat cttttatata atcgggcata
    21661 tgaagtatga ttgtatctat tttttgatat atttctgtat ttttcatgtc attcatcctt
    21721 tcaataagta ggggagaaga gtatctctat aattggacta taccaattat agaatgtttt
    21781 gtcaattggt atgctctaaa atttattaat ttgtttcaaa aaaagcatat aaacaggtat
    21841 acgtttaata atttaaaata aatggtatac ataaataggg aaggaaatag aagggagcaa
    21901 tcta
//
LOCUS       DAYYME010000039        21619 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000039 DAYYME010000000
VERSION     DAYYME010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.039"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            185..1609
                     /locus_tag="ACHBFW_001221"
     CDS             185..1609
                     /locus_tag="ACHBFW_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM9317534.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(1651..1908)
                     /locus_tag="ACHBFW_001222"
     CDS             complement(1651..1908)
                     /locus_tag="ACHBFW_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317535.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            2070..3179
                     /gene="pdhA"
                     /locus_tag="ACHBFW_001223"
     CDS             2070..3179
                     /gene="pdhA"
                     /locus_tag="ACHBFW_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFM9317536.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            3182..4159
                     /locus_tag="ACHBFW_001224"
     CDS             3182..4159
                     /locus_tag="ACHBFW_001224"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFM9317537.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            4184..5827
                     /locus_tag="ACHBFW_001225"
     CDS             4184..5827
                     /locus_tag="ACHBFW_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFM9317538.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            5835..7241
                     /gene="lpdA"
                     /locus_tag="ACHBFW_001226"
     CDS             5835..7241
                     /gene="lpdA"
                     /locus_tag="ACHBFW_001226"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFM9317539.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            7403..7678
                     /locus_tag="ACHBFW_001227"
     CDS             7403..7678
                     /locus_tag="ACHBFW_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFM9317540.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            7684..8451
                     /locus_tag="ACHBFW_001228"
     CDS             7684..8451
                     /locus_tag="ACHBFW_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFM9317541.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            8619..10448
                     /gene="typA"
                     /locus_tag="ACHBFW_001229"
     CDS             8619..10448
                     /gene="typA"
                     /locus_tag="ACHBFW_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFM9317542.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            10709..12481
                     /gene="recQ"
                     /locus_tag="ACHBFW_001230"
     CDS             10709..12481
                     /gene="recQ"
                     /locus_tag="ACHBFW_001230"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFM9317543.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            12595..12897
                     /locus_tag="ACHBFW_001231"
     CDS             12595..12897
                     /locus_tag="ACHBFW_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFM9317544.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            12900..14063
                     /locus_tag="ACHBFW_001232"
     CDS             12900..14063
                     /locus_tag="ACHBFW_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM9317545.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            14089..17517
                     /locus_tag="ACHBFW_001233"
     CDS             14089..17517
                     /locus_tag="ACHBFW_001233"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFM9317546.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            17538..18662
                     /locus_tag="ACHBFW_001234"
     CDS             17538..18662
                     /locus_tag="ACHBFW_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFM9317547.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            18643..19089
                     /locus_tag="ACHBFW_001235"
     CDS             18643..19089
                     /locus_tag="ACHBFW_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM9317548.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            19073..19411
                     /locus_tag="ACHBFW_001236"
     CDS             19073..19411
                     /locus_tag="ACHBFW_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFM9317549.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            19530..20090
                     /gene="rsmD"
                     /locus_tag="ACHBFW_001237"
     CDS             19530..20090
                     /gene="rsmD"
                     /locus_tag="ACHBFW_001237"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFM9317550.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            20087..20578
                     /gene="coaD"
                     /locus_tag="ACHBFW_001238"
     CDS             20087..20578
                     /gene="coaD"
                     /locus_tag="ACHBFW_001238"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFM9317551.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            20568..21611
                     /locus_tag="ACHBFW_001239"
     CDS             20568..21611
                     /locus_tag="ACHBFW_001239"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFM9317552.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT         7329 a         3748 c         4747 g         5795 t
ORIGIN      
        1 ttatatgttt tgtttggctg gaattattat cgcagataca agcttttgaa catcattatt
       61 cagaataagg gactgtgata agatttttgt cacagtccct tatgttttgc aaaaaaagtt
      121 tttctttttt ctcgtaaatt cggtaaaatc aaaagaaaaa atagacaata gaaaagagaa
      181 gcttatgtgg gaaaaagtag agaaaaaaca aggaccgatt tatcgtcaaa ttatggaaca
      241 gattatgaga gaaatcgaaa acggaaggat caatcctgga agtcaacttg aatcagaacg
      301 aaaactaggg caactttttg gtgtgaatcg ttccaccgtt gtccatgcat tggaagagct
      361 tagggagctt ggggtactaa cgagcaaacg tggcagcgga tggtttgtta atcagactga
      421 atgggggaaa ttcgctgttc caagaatcga ttggcggcaa atgatctctc ctcgttatga
      481 gcagacggac tggtatgagc aaaaaataag agaagcaaag cagatccaaa aaacatcgtt
      541 tcttgattta tacgctagcg agatgccaga tgagcttttg ccgaattttg agtttccttc
      601 ttattctctt gaagaaatct tagcggaaga aaaagaaatg gatcttttag gctatactcc
      661 gttacgtaac aaaatcaaac ggtatttgga aagtaaattt gaattggcat tttcccccaa
      721 ccagcttctt gtgactggtg gcggtcagca agcaatcttt ttgattttac aaacgattct
      781 ttcagctgga gaggggatag ctgtagagtc accttctttt ttttaccgat tacctctttt
      841 caaagcttca ggaacgcgtt tatttgggat accgatggat gaagaaggga tcgacttaga
      901 aaaactgaaa gaatcgatcc ataaaaataa attgaaagca gtgcttatca acccgaattt
      961 tcaaaaccct acaggaaaag tgatgagtca aaaacgacga gaaaagctag tagaattgtg
     1021 tcgacagtat cggcttccaa ttattgaaga cgatgtattt acggatcttt catttctaaa
     1081 ccaagaagaa gtccttccta tacgaacgat tgatccggat aatgtgttat acgtcggttc
     1141 gttatctagg gttttaggaa aaacaacgaa aatcggttgg gtgataggac ctgagacttt
     1201 tatctcgcaa ctagctcaag cgcaagaaat gatggagttt tcgatgaata tccttacaca
     1261 aatcacagtg gctaatgtat ttaatgaagc aattgatttg aaaatgcaag atgtaagaaa
     1321 aaagttgaaa gaaaaaagcc gactgataag cgcttgggga gattcacaat cgtttttcaa
     1381 gcttcattca attttaggtg gttactatgc ctggattact tgggatggga aaaaactaga
     1441 ccccgaacta gcagaaaaag tagtagatat gggtcttgga attgcaccaa gtctactttt
     1501 tgggcaagat ataaatggaa tcagaatcaa ttacagtagg ctggatgaaa accaaatacc
     1561 gttgttttat tcaaagatga aaattctaga aaagtggtta acagaataat ttcacaaagt
     1621 aaatagatta aagaatataa cagcttggat ttaaatttca agctgtttct tttttttcgg
     1681 aaaaagaata cgatataaag aaactaagaa aaaaccatta tttgtacgtc cgttcacgat
     1741 gtgtaaataa aataaatgtt tgcaacaaaa ctgtataaca ccaacgtttt taaatagaaa
     1801 cagatggttg taagtttgat tagaaaatag aaaaaatgca gatgaaatat aaaacgcttt
     1861 ccttattgtg attttattca ctaatgctgt taatatgcgg attggcataa tttcctttca
     1921 tcgtgaaaat ttgtgctttt agcttgtatt tctcataaat ccatgataat ttattaatga
     1981 agacggttaa atagtgatac agttttattc actgtttcaa tcgcagaatg aagagatttt
     2041 cttatagatg agagaggaat gtgtgacaaa tggcaaacaa aaaagcaccg atcgacttcc
     2101 aagcactgct agatgaagtc aatgcagatt tcccagtata tcaagcactt gatcaagatg
     2161 gaaacgtagt taacaaagat ttagtacctg atttatccga tgatgaactc gttgaattaa
     2221 tgactcatat ggtatggtct cgtgtattag accaacgttc aacagcgctg aaccgccaag
     2281 gacgcctagg cttttttgca ccaactgctg gacaagaggc aagtcagctg gctagtgctt
     2341 acgcatttga taaagaagat gttcttcttc caggttatcg ggatgtacct caactagtaa
     2401 aacatggttt acctttatct caagcttttt tatggtctcg aggacatgct gcaggtaatt
     2461 cttatccgga agaattgaaa gctctaccac cacaaattat catcggtgca caatatgtac
     2521 aagcagccgg tgttgcatta ggtttgaaga aacgaaacaa gaaaaatgtc gtcttcactt
     2581 atactggtga tggcggttca tcacaaggtg atttttacga agcaatcaat ttcgctggcg
     2641 cttaccatgc aaatgccgta ttttatatcc aaaacaacgg gtatgcgatc tctacacctc
     2701 gtgaaaaaca aactgcagca aaaacacttg cacaaaaagc tgtcgcagct gggattcctg
     2761 gtattcaagt cgatggaatg gatccgcttg cagtatatac tgtatctaaa atggcacggg
     2821 aatgggcgat cagtggaaat ggaccagtcc taatcgaaac actgacttat cgttatggac
     2881 cacatacact atctggtgac gatccaaccc gctaccgtac aaaagacaca gatgacgatt
     2941 ggcagaaaaa agatccattg atccgcttcc gtaaatattt gacggaaaaa ggactttggt
     3001 ctgaagaaaa agaagaacaa gtcatcgaag caactaaaga agaaatcaaa gcagctattg
     3061 ctgaagccga caaagtacca aaacaaaagg tttcagattt cttgaaaaac atgttcgaag
     3121 tatctccaca atcaatcaaa gaacaaattg caatttatga agcgaaggag tcgaaataat
     3181 catggcacaa aaaacaatga tccaagcaat cacagatgcc ttagcacttg aactagaaaa
     3241 tgacgagaat gtcgttgttt ttggtgaaga tgttgggaaa aacggaggag ttttccgggc
     3301 aactgaagga ttgcaggaaa agttcggaga agaccgagta ttcgatactc ctttagctga
     3361 atctggaatc gcaggattga gtttcggtct tgcgttagaa ggatttcgtc cagttcctga
     3421 aatccaattc tttggtttta ttttcgaagc aatggacgaa gtagtagctc aaatggctcg
     3481 tacaagatat cgaatgagcg gcactagaaa tctacccatc acaattcgtt cgccatttgg
     3541 tggcggtgtt catacaccag aattgcattc ggataattta gaaggtttga tcgctcagtc
     3601 tcctggtatt cgagtagtga ttccttccaa cccttatgat gcaaaaggac tattgatttc
     3661 ttctattaga agtaacgatc cggttgtttt cctagaacac atgaaactat atcgctcatt
     3721 ccgagaagaa gtgccggatg aagcttacga agttcctttg gataaagcag cagttactcg
     3781 tgagggaaca gatgtatcta ttatcacata cggagcaatg gtgcgtgaag caatcaaagc
     3841 agcagacaat cttgcaaaag aaaatatatc tgttgaaatc attgatcttc gtacagttgc
     3901 gccattagat gtagaaacga tcattcaatc cgtggaaaaa actggtcgcg tagttgttgt
     3961 ccaagaagca caacgacaag cgggcgttgc cgcgcaagta gtttctgaga tctcagaacg
     4021 cgctatcctt tcattagaag ccccaatcgg acgagtttct gcacctgata cagtcttccc
     4081 atttggacaa gcagaaaatg tctggttgcc aaatgcaaaa gatatcgaag acaaagtaaa
     4141 agaaatcgcg gaattttaag aaaagaaggg acgaaacaaa gtcatggctt atcaatttaa
     4201 attgccggat atcggtgaag gaatcgcaga aggtgaaatc gtcaaatggt tcgtcaaacc
     4261 aggagatacg atcaatgaag atgatacatt actagaagtt caaaatgaca aatcagtaga
     4321 agaaatcccg tcacctgtta caggaacggt taaaaatgtg attgttccag aaggtaccgt
     4381 agcaaatgtt ggggatgtat tagtagaaat cgatgcacca ggacacgaag acaatgaagg
     4441 ggacagtggt gtagccgcag aatcccaaac tccagctaaa ccggctgctg aacccactgt
     4501 tgatacagaa tcagctggat cgtcaagcga aggagtcttc caatttaagt taccggatat
     4561 cggtgaagga atcgcagaag gtgaaatcgt caaatggttc gtcaaaccag gagatacgat
     4621 caatgaagat gatacattac tagaagttca aaatgacaaa tcagtagaag aaattccgtc
     4681 acctgtcaca gggacagtta aaaacgtgat tgttccagaa ggcactgtag caaatgttgg
     4741 ggatgtatta gtagaaatcg atgctcctgg acacaatagt gcaccgtcaa ctagtgcgcc
     4801 atcagctgaa gcaccaaaag aaaaagtgga aacatcaggt tcagcaagtg tcgttgaagc
     4861 tgccgatcca aataaacgtg tattggctat gccatctgtt cgtcaatttg cccgtgaaaa
     4921 agatgtagat atctcacaag taactgcaac tggaaaaggt ggcagagtaa caaaagagga
     4981 catcgaaaac ttcttggcag gtggtccaag cagtgcacca gcgaaatcag aagcgccaga
     5041 agcggcagca ccgaaagaag cagctcctgc agcagaaagc aaaccagctg caccagcaaa
     5101 accattcaag tccaacttag gagacttgga agaacgtgta gcaatgacgc caacacgtaa
     5161 agctatcgct aaagcaatgg tcaacagtaa gcatacagct ccacacgtga cattgcatga
     5221 tgaagtagaa gtgtctaaac tatgggataa tcggaaacga tttaaagaag ttgctgctgc
     5281 aaatggtacg aaacttactt tcttgccata tgtcgtgaaa gctttaacag cgactgttaa
     5341 gaaataccca gtattgaatg catctatcga cgatgcaaac caagaaatcg tttataaaca
     5401 ttactacaat atcggtattg caactgatac ggatcacgga ctatatgtac caaatgtgaa
     5461 agatgcagac cgcaaaggaa tgtttgctat tgcagacgaa atcaatgaga aagcaaaatt
     5521 agctcatgac ggcaaactat ctgcagaaga tatgcgcaat ggaacaatta cgatcagcaa
     5581 tatcggatct gtaggtggcg gttggtttac accagtcatc aactatccag aagtagctat
     5641 tttaggtgta ggaacgattg cacaacaacc aatcgtaaat gctgaaggcg aaatcgttgt
     5701 cggtcgtgtg atgaaactgt cattaagttt cgatcaccgt atcgtagatg gagcaactgc
     5761 gcaacaagca atgaataata tcaaacgtct tttagctgat ccagagctat taatgatgga
     5821 aggatgataa acaaatggta gttggagatt ttgctgtaga attagataca gttgtaattg
     5881 gtgcgggacc tggtggctat gtagctgcca tccgcgctgc tgaaatggga caaaaagtag
     5941 caatcattga aagagagtat attggcggtg tttgtttgaa tgtcggctgt attccttcaa
     6001 aagcactgat agctgcagga caccattatc aagagtcact agattctgaa atgtttggag
     6061 taactagtga aaacgtcaaa ctagattttg caaaaacaca agaatggaaa gaaaacaaag
     6121 ttgtccatac gttgacttct ggtgttggtt tcctattaaa aaaacataag gttgaaacaa
     6181 tcgaaggaga agcgttcttc gtagatgatc acacactacg cgtcattcat ccagattcag
     6241 ctcaaactta ttcgttcaat aacgcaatca tcgcaacagg ttcccgtcca atcgagattc
     6301 caggatttaa attcggagga cgtgtgttag attctactgg cggattagca ttgaaagaag
     6361 tacctaagaa atttgtgatc atcggtggcg gtgtgattgg tgccgaattg ggcggagcgt
     6421 acgcaaactt aggagcagaa gtaacgattc ttgaaggctc accacaaatt ttaccaactt
     6481 acgaaaaaga tctagtaaaa ttagtagaag atgatttcaa gaaaaaaggc gtaacagtcg
     6541 taacgaatgc aatggcgaaa gaagcagtcg ataacggtga cagcgtgaca gtgaaatatg
     6601 cagtggacgg gaaagaagaa tccgttactg ctgattatgt gatggtcact gtaggtcgtc
     6661 gtccgaatac agatgatatg ggcttagaac aagcgggtgt cgaagtagga gaacgaggat
     6721 tgatcacggt ggataaacaa ggacgtacga acgtacctaa catctatgcc atcggtgata
     6781 tcgttccagg ggcggcatta gcacacaaag ccagttatga agcaaaaatc gctgctgaag
     6841 caatctctgg caaaaaagta gctgtagatt ataaagcaat gccggcagta gccttcactg
     6901 atcctgaatt ggcttctgta ggtatgacga tcaaagaagc aaaagatgct ggtatcgaag
     6961 caaaagcata taaattccca ttttcaggga acggtcgggc attatcgctt ggtaaaacag
     7021 aaggattcat ccgtctagtt acgacgatcg aagataacgt gcttattggc gcgcaaatcg
     7081 gtggagtcgg cgcaagtgat atggtctctg aattagcttt agcaatcgaa tcaggaatga
     7141 atgctgaaga tatcgccttg acgattcatc ctcatccatc tcttggcgaa atcgtcatgg
     7201 atgcttcgga attagcatta ggtttaccaa tccatatttg atttgaataa tacaggcgaa
     7261 acgcaactga aaaatcagtt gcgttttgtt ttttagaatt atcaataata ttattatgta
     7321 aacttaaaaa tatgacaact tcttggttta ttaccacttc ctatgctaca ctgaagagga
     7381 atatgaaata ggagagttga agatgcaaga atatcagtat ccacttgatt tagattggac
     7441 aacagacgaa atggtcattg taatgaagat gtgggaagat ctggaacgtg cgaatgaaaa
     7501 agggataaac aatgaagctt ttttgaagac ttatcagcag tttaaaactg ttgtcaaatc
     7561 cattggtgag gaaaaacgat tgggacggga atttgaaaaa gcttccggtt attctttata
     7621 tcgtacagtg cagcaagcaa aaaaaaatcc aaacaaaact ttgagaatga acgggtgaga
     7681 taaatggaac agatgattga tcatatcaag tcctggcttg aagaagctgg tgagaaaatc
     7741 aaatcttctc aacaaacatt aacaatctcg caaaaatcaa atcgaacgga tctagtaaca
     7801 aatgtcgatc gaaacatcca gaattttcta atcgaaaaaa tccattcctt tgatccagag
     7861 gcgaaaatat tggcagaaga agaagggtgt aatcaattat cggatctcga tggaagagtc
     7921 tttatcatcg atcctattga tggcaccttg aattttgtga ttgaagggga aaatttttgt
     7981 attatgctcg ctgtttatga atccggcgtt ggaaagcttg gtttcattta tgacgtgatg
     8041 agaaatgaac ttttttgggg tggaaaagga ataggcgtgt ataaaaatga tgagccattg
     8101 cctagaccag caaataaatc cttatcggaa ggcttacttg gtgtgaatag ttatcttttc
     8161 ggtcataata aatatggaat ccgagaaatt gcggaaagca gcatggggac acgtatgtac
     8221 ggatgcgctg gtcttgaact agtggcaatc ttgaaaggaa accacattgc ttatatttca
     8281 aaccttagcc cttgggatta tgcagcggga aatgtcttat tggaagagtt tggtatggta
     8341 acaagcggtc tttttggtac tcctttgaca tttgaaaaaa gggaatacta tttagcaggg
     8401 acttcttgtg catatgcaga aatcgcagac cttttgaaaa aagaaggctg aaaaaaaggt
     8461 agaaatcatt cacaaaaatg ataaaaacga gcaaaagatt aattttctcg aaaaaatgaa
     8521 aacattttac tatatttcat gaaaaaagtc tgctataatt agcagtcgag taaaatttgc
     8581 cctgtcataa agaaccaata aaaaaggagt tagttacatt gaattataga aacgatatcc
     8641 gtaacgtcgc aattattgcc cacgttgacc acggaaaaac aacattagtt gatgaactat
     8701 taaaacaatc agatacatta gacgcacata cgcaattgca agaacgtgcg atggactcaa
     8761 atgcgctaga aaaagaacgt ggtatcacga ttttagccaa aaacacagct gtagactata
     8821 aaggtattcg cgtaaacatc atggatactc caggacacgc ggacttcggt ggcgaagtag
     8881 aacggatcat gaaaatggtt gacggcgttg tgttggtcgt agatgcgtat gaaggaacaa
     8941 tgcctcaaac acgttttgta ttgaaaaaag cactggaaca acatatcaca ccaatcgttg
     9001 tcgtaaacaa gattgacaaa ccttctgctc gtcctgagca tgtcgtagat gaagttcttg
     9061 aactattcat cgaattagga gcagatgatg atcagttaga ctttcctgtg atttatgcat
     9121 ctgcactaaa tggtacgtca agcttgtcag atgatccagc agatcaagaa ccaaccatgg
     9181 cacctatttt tgatacaatt attgaaaaaa tccctgctcc tgtcgataac agcgacgaac
     9241 cattacaatt ccaagtttca ttacttgact acaacgatta tgtaggtcgt atcggaatcg
     9301 gacgtgtatt tagaggaaca atcaaagtag gagaccaagt agcattaatt aaattagacg
     9361 gaacagttaa gaaattccgt gtaacgaaat tatttggttt ctttggacta aaacgtctag
     9421 agattcaaga agcaaaagct ggagacttga ttgctgtctc aggtatggaa gatatctttg
     9481 tcggtgaaac agttactcct gtggatcatc aagatgcgtt acctatcctt cacattgacg
     9541 aaccaacatt gcaaatgact ttcttagtta ataattcacc atttgccggt cgagaaggta
     9601 aattcgtaac cgctcgtaag attgaagaac gtttgatggc agaacttcaa acagatgtat
     9661 ccttacgagt tgaaccaaca aactctccag atgcatggac agtttctggt cgtggagaat
     9721 tgcatttgtc tatcttgatt gaaaacatgc gccgtgaggg atacgaattg caagtatctc
     9781 gtccagaagt tattgaaaaa gaaatcgacg gtgtaaaatg cgaaccgttt gaacgcgtgc
     9841 aaatcgatac accagaagaa tatatgggta gtgttatcga gtccttgagc ttacgtaaag
     9901 gtgaaatgca agatatggtc catacaggaa acggacaaat ccgtttgaca ttcttaacac
     9961 ctgcccgcgg tttgattggt tattcaactg aattcctttc aatgacacgt ggttacggaa
    10021 tcatgaacca tacttttgac caatatctgc caatgcttcc tggtcaaatt ggcggacgtc
    10081 accaaggggc gcttgtttcg atcgatacag gaaaagcaac gacttattct atcatgagta
    10141 tcgaagaacg cggtacagtc ttcgtagaac caggtacaga agtatatgaa ggaatgatta
    10201 tcggggaaaa cagccgtgat aatgatttga ctgtcaacat cacgaaagca aaacaaatga
    10261 caaacgtacg gtctgctact aaagaccaaa catctgtcat caaaaaacca aaacaattga
    10321 cgttggaaga atctttggaa ttcttgaatg atgatgaata ttgcgaagta acaccagaaa
    10381 gcattcgctt gagaaaacaa attttagaga aaaatgctcg tgaaaaagca tctaaaaaga
    10441 aaaaataatc tgtaaaagga tgaagagaga aaccagcttg ttataaaaaa tagctggttt
    10501 ctttttttgt ccaactcttt tctgtggaga agtcatcttt gtcaatggac ataaattcag
    10561 aaaatacgcc gaaagacgtt taaaagactt ttaaaaaact tatttctaga gagtttttgt
    10621 ttcataataa gataatgttt cacgaaagga gatcaacttg tccttcgaag aaaatcgaag
    10681 taaaatggca agagaaagga gcgggaagat gaacgactta ttaaaacaat actttggcta
    10741 tgatgagttt agacctggtc aaaaagaaat tatccagaaa gtcattgatc aggaaaatgt
    10801 attaggaatc atgcctaccg gaagtgggaa atcaatctgt taccagcttc cagctttact
    10861 attagatggg cttactgtgg ttgtgtcccc tttaatctct ttgatgaagg atcaggtgga
    10921 cgcggcgaat caattaggta tacccgccac ttttatcaat agctcgttag atggttacga
    10981 aacagctaga cgcttccaag aaatcgatag acagcagtac cgtttacttt atatcgctcc
    11041 tgaacgtttt atcatgccgg attttatcca agcaatgaag cgatggaacg tccgtatgat
    11101 cgctattgac gaggcacatt gtatctctca gtgggggcat gatttccgcc caagttattt
    11161 acagatggcg aaccagctag atcaactgcc aaatcgtcct gtaattgttg ctttgacagc
    11221 tacagcgact gtgcaagtag ctgctgacat aaaaagactt ttaaaaattc cagaaaacaa
    11281 ccatattcag actggttttg aacgagaaaa tctacgattt caagtaatta aagatcaaaa
    11341 aaaagaacaa tatctaatcg aatatttaaa gatcaacaaa aatcaatctg gtatcattta
    11401 tgcagcaacg agaaaagaag tagaccgtct ctatcatttg cttaaaaaat tcgatttttc
    11461 cgtgggtaga tatcatggtg gtctaaatga gaatgagcga acagaaatgc aagaagcctt
    11521 tttatacgat cgcttgcagc tgattgtagc gacaaatgca tttggtatgg gtataaataa
    11581 aagcaatgtc cgttttgtta tccattatca aattcctggc tctctagaag catactatca
    11641 ggaagctggt cgggcgggaa gagatggtct ttccagtgaa gccatcctgc ttttctctcc
    11701 tcaagatatc caagtccaaa agttttttat ccagcagtcc caaagagaag aagggcaaaa
    11761 gcaaaaagaa tatgaaaaat taaaagcgat gacagaatac gtgtatattg agtcctgtct
    11821 tcagcaatac atcctaaact attttggcga gacaagcagt ccgtgcaacc gttgtggtaa
    11881 ctgtttagat gacagagaaa tagtagaagt aacgacagaa gcacagatgg ttctttcttg
    11941 cttaaaacgt atgggtgaga actatggtaa acaaatgctg atgaaagtct tagcaggatc
    12001 gaaagaacaa aagctgcaag ctttaggttt tggacattta tctacctatg gactgatgaa
    12061 aaaccaatcg caaaaagaaa cgatgcagtt aattgaatat ttgatatcga atggctatct
    12121 tctgaccatc aatggtgagt atcctgtttt gaaagtaaca gaaagaggga tacaagtatt
    12181 gaaaggacaa gaatctgttt atcgaaagga acccaagaaa gtccagcagc tatcggatga
    12241 agaaaccgat acactatttg aagtgctgcg ggaactgcga acggatctag catcagaagc
    12301 aggcgttccc ccgtatgtcg tattttcaga ttccacattg aaagaaatga gccgaatacg
    12361 tccatcaagc agattggaga tgcttcagat aaaaggagtt ggccaaagca agcttgataa
    12421 atatggcgaa gcctttttat ctcggataaa aaatgcggac tctaacgttc tggataagta
    12481 gaaagaacga atacgtaaaa atgaataaaa acaactggtt aaatctgttt tttttgctcc
    12541 ttatgaaaat caaatatgtt ataattaagg aattagaatg ggaggtaagc acagatggat
    12601 tctttgtcga aaaaacttgc gatccaagta ttaaatgatg aagcagaacg gatcaaacga
    12661 ttaatccgaa atcaaaacaa cagtctctgt atctcacaat gcaaagcctt cgaagaagtg
    12721 gtagatacac agatgtacgg cttttcccgg caagtcggtt ttgctgtccg tataggagta
    12781 atcggaaaga acgagggcca gctgatgtta agtgagctag aacgagaatt gaatcagtta
    12841 tatagtgagg tttaccaaga aatttatgat aaaaaagaag ttggcaagga ggaatagact
    12901 tgcctaagaa agtgaaaaaa aggcatctat tagactatag catcttgatt ccctatttaa
    12961 ttttatgtgt attgggattg atcatggtat atagttctac gtcgtatcta ttattagaaa
    13021 acggacaaaa tccgtccgca tcagtcatta accagagtat tttttgggtg cttagcttga
    13081 ttgctatcgc actactttat aaaatgaaaa cagatgtttt gaaaaaccag cgtttgatca
    13141 tggcagccat cgcagttttg acgattttgc tgctgattgt tgtgtttttt ggtaaggaga
    13201 tcaatggggc gaaaggttgg ctgcaaattg caggattctc gatccaacct gccgaatacc
    13261 taaaaatcat ttctatctgg tacctgtctc tgactttatc taaaagacaa aacagtgtac
    13321 aaaaagattt tcttgggact gtgaaacgtc cacttgcaat ggttatcgga cttactgcat
    13381 tagtcgctat tcttcccgat tttggtaatg cggcagttat ctttttgatt attttggttc
    13441 tgcttttagc cagcggagtc aattatgtgt atactttgat tgtaggagta ggaggctttt
    13501 gtctaagtac ttttacgatc tggctgatca atatcacaaa cggaaagatc ttacccggac
    13561 gtttacagta tatctataat cgatttgcta tttaccaaaa tcctttctca gacgaattaa
    13621 ataaagggca tcaactagtc aacggctact atgccatgtt caacggagga ctatttggca
    13681 ggggattagg taacagtatc cagaaaaaag gatttttaca agaagcacaa accgatttta
    13741 tttatgcaat cgtagtggaa gaactgggag tgattatggg gattttgata ttagcgttgc
    13801 tattctttat gattgtgcgc attgtccttg taggtatccg ttcaaaagat ccgttcaatt
    13861 cgcttttgtg cattgggatc ggcgcaatgt ttttgatcca agtatttgtc aatcttggag
    13921 gaattactgg cgttattcct ttgacaggga tcacgttccc atttttaagt caaggaggct
    13981 ccagtttgct gatgctttcg atttgtgtcg gctttgtcct caacatcagt gcggatgaaa
    14041 aacgcaagag cttaggttta taataaaaaa agatgtagga gtggttcgat gaaaaaagtt
    14101 ttagttgcta acaggggtga aatcgctgta cgtgtatttc gtgcttgtac agaattagga
    14161 attaaaactg ttggaatcta tgcagaagaa gatgaatatt ccgtacaccg tttcaaagcg
    14221 gatgaagcat atttagtagg acaaggaaaa aaaccgattg atgcgtatct agatattgaa
    14281 gggatcattt ctatcgcgaa agaatgcgga gctgacgcta ttcatccggg ttacggtctt
    14341 ttgtcggaaa acttgaattt tgcgcaacgg tgcgaagaag aaggcatcat ttttgttgga
    14401 cccaaactgc atcatttaga tatttttgga gataaaatca aagcaaaagc tgcagctatc
    14461 gaagcgggga ttgcttcgat tccaggcaca gatggaccaa tcgcttctat agacgatgcc
    14521 ttggagtttg cgaaacaata cggttatccg attatgatca aggcagctct aggcggcggc
    14581 ggacgcggca tgcgtgtagc tcacgatgaa aagagtgcaa gagagggcta cgaacgtgcg
    14641 aaaagcgaag caaaagcagc ctttggtagt gatgaagtgt atgttgaaaa atacatcgct
    14701 aatcctaagc atatcgaagt acagattctc ggggatacgc atggaaacgt catccatctt
    14761 tttgaaagag attgttctgt tcagcgccgt caccaaaaag tagtagaagt agcgccttgt
    14821 gtctctatga atgaacagca acgtcaaaaa atctgccagg cagctgtcca attgatgaaa
    14881 tatgtcgggt atgtcaacgc aggtacagta gaatttcttg ttgaaggaga tgatttttac
    14941 tttatcgaag tcaaccctcg cgtccaagtg gaacatacga tcacagaaat gattacagat
    15001 attgatatcg taacaacgca actgttgatt gctcaaggtt tggatctgca taaagaaatc
    15061 ggattgcctc agcaagaagg aattaaatta aatggttcag ccattcaatg ccgaatcact
    15121 acagaagacc cgttgaataa ctttttacca gacacaggga aaatcgatac gtatcgttca
    15181 cctggtggtt ttggcgtacg tctggatgtc ggaaatgctt acgcgggcta tgtagtgaca
    15241 ccgtattttg attcattgct agtaaaagta tgtacgcatg gtgctacctt tgaaacagcc
    15301 attcagaaaa tggaacgttg cttgaaagaa tttagaatcc gtggagtgaa aacaaacatt
    15361 ccatttatgc tcaatgtcat cacacatcct gaattccaat caggaaatgc aaaaacaacc
    15421 ttcattgata gtacagcaac attatttgaa tttcctcgac tacgagaccg cggaaacaaa
    15481 acgatgaaat atatcggtga aattactgtg aatggttttc caggtatcga aagtggcgaa
    15541 aaaccatttt atgaagaacc acgtatgcct aaagatctaa tcacaagaac tgattacgta
    15601 acggctaaga atgtattaga tgcgaagggt gccgatgctt tggtcgaatg gatcaaagga
    15661 caggaaaatt tgttgttgac ggatacgaca tttagagacg cccatcaaag tctgctagca
    15721 actcgtgttc ggacaaagga tttcaagcag attgcacgtt tgactggaga aggtcttcct
    15781 gaactctttt caagtgagat gtggggggga gcaacatttg atgtggctta ccgctttttg
    15841 aatgaagatc cttggcaacg gttgagaaaa attcgttctt taatgcctaa tacgttgctg
    15901 caaatgcttt tcagaggttc aaatgccgtt gggtattcta attatcctga caatgtattg
    15961 gtggaatttg tcaaagaagc agcagcgcaa ggaatcgatg tatttcgaat ctttgatagt
    16021 ttgaactgga ctccacaaat ggaaaaaagt atccaagctg tacgggatac tggtaaaatc
    16081 gcagaggcag ctatttgcta tactggcgat atcaacgatc caagtcgggc aaaatacaat
    16141 gttcagtatt ataaagacat ggcaaaggaa ctagaacaac tgggtgcgca tatcattgca
    16201 atcaaagata tggctgggct gcttaaaccg caggcagctt atcgtttgat tagtgaattg
    16261 aaagaaacga cagatctgcc aatccatctg catactcatg ataccagcgg gaatggaatc
    16321 atcacttatt ctgctgcttc aaaagcagga gtagacatcg ttgacgtggc aatgagtgca
    16381 atgagtggga atactagtca gcctagcatg agcagcttgt attatgcact ggtcaatggt
    16441 ccacggctgc cagaaatcac gattgaaaat gcacagaaat taaaccatta ttgggaagat
    16501 gtgcgcatgt attacaaacc gtttgaaaac ggcttgaatg ctcctgaaac agaagtatat
    16561 atgcatgaaa tgccaggagg acagtattcg aatctacagc aacaggcaaa agctgtcgga
    16621 ttaggtcatc gctgggatga aatcaaacaa atgtatcata ctgtcaactt gatgtttggc
    16681 gatatcgtta aagtaactcc atcatctaaa gtagttggag atatggcact atttatggtc
    16741 caaaatgatc ttacagaaga agatatttat gaaaaaggcg aaacactaag tttccctgaa
    16801 tctgttgtga ctttcttcca aggagaacta gggcaacccg ttggcggatt tcctaaaaaa
    16861 ttgcaaaaga ttattcttaa aggcagacct gcattgaatg aacgtccagg tcttttggct
    16921 gaatcagtcg acttcaacga agtaaaaaaa gaattggcag aaaaaattgg ctatgaacca
    16981 aaacaagacg aagtattaag ctatttgatg tatccgcaag tctttttgga ctatcaaaaa
    17041 gcatataatc aatttggcga tgttacatta ctggacacac caacattttt ccaaggtata
    17101 cgtttgggtg aaacaatcaa tgtacaaatc gaacgcggaa aaacgcttat cattcgttta
    17161 gatgaaatcg gagaacccga tatcgaaggg aatcgcgtac tgttcttcaa tttgaatgga
    17221 caaagacgtg aaatcactat caaagataat tcgatcatca gtacggtcca aacccgattg
    17281 aaagctgaac cgacaaaccg agaacaaatt ggagcaacga tgtctggttc tgttttggat
    17341 gtattagtga aaaaaggcga taacgttaaa aaaggtgata cgttgatgat tactgaagcg
    17401 atgaaaatgg aaacggccat cgaagcacgt tttgacggag aagtcgcaca tgtatatgtg
    17461 tcttcaggag acactatttc gtctggtgac cttttgattg aagtgactga gaaataataa
    17521 gcgaaaggtg gggaaacatg aaaagactgg gtttgttttg gctgattctg ctattagtta
    17581 ttgtggcatt ctacatggaa ccagtcttgt ttccgccaaa acttgaagtg aaggaaaact
    17641 ctgatcaaca aatatcaaac caaatgacaa cgtggaaata ccaggagctt aaagcaactg
    17701 gtttttctac ttatatcgga aaaccgatcg atcagctgga aaaggatttt ggccaagcct
    17761 atgatcgatt aactagcgga tttggttttg aaacaagata ttatgaaaat aaagctagtc
    17821 atctcacatt tgaagcgaac atccaagaca gcagagtcac aactgttaaa gtactgcaaa
    17881 cagggcctgc tgatatcgcg ccgttttatt tagggatgac gatgcaagat cttacgaaaa
    17941 tcactactat ctatactaat ttcacttttg agtaccatga tacagaagtt ggaatcgaac
    18001 taatggaaga ggatatgaat tatcgtccgc taattgcttt tgataacgac acttttgcta
    18061 ttttattttt tgatcaaaca acaaacggcc tattttcagt tgtgtattta gataaagata
    18121 gtcttttgaa actattgcca tatcaagtgt ttggtgaagg ccttccacat tatcaagcag
    18181 ataaatcggc tgattgggaa tcaatcaatc gcgttaaaga acgaaaaagt acgactttat
    18241 taaatatgct tagaagagaa gacgaacttc ccatatacaa tcaaacactt gcctttcaaa
    18301 accacagtca gctgctacta catgattttt tgacaaggcc tgaggaaata ttgtcagagg
    18361 aacgtatggc tgattggcaa aaatcgctag gcagtgctca aacgacagtg aaattctcac
    18421 tgacgaatga tgaattggag aagttaatca gtaaacaaaa attagaaaat actagtgggc
    18481 tatttattca tccggcgatt gacgctactt tttcgttttt atattggtac agcgatcctt
    18541 acaaccatga ccgtttcatg gacccgggaa cggattctgt aggcattgct ttttctaaag
    18601 aaaatatgtt agtcttattg caagaagaaa acggagaaag tagtgatagc agtgatcgtt
    18661 aacgaagagc tgtttcaact ggaggatcaa tgcctccaag tcgctgaaat gataaaaaag
    18721 agtaaaacga tggcttctta tcttgaacat aaacagtcaa tggaagaatc tgcaaaagtt
    18781 tgccagttga aagaagcgtt cttgtctaag aaagaagcat ttgaaaatgt agcggcatat
    18841 ggtgagtatg cacctgatta tcgtgaaaaa aaacgagctg ttcgaaaagc aaaaagacag
    18901 cttgatatgg atgagcatgt tgcagctttc cgatatagtg aaacacagct gcaaagtctg
    18961 ttggatcaaa ttgtccaagt acttgccgaa gaagtttcta atgaaatcaa agtagatgca
    19021 ggtagcccgt tttttgaaac gaaaaaacac tttggatgcg gaggtaattg tcatgcaatt
    19081 agacaatagt gaatttgaaa taacaaaacg acgcgggctg atcgtatggg tctacagctt
    19141 aaaacaatta aagaatctga aaaagttcgg cttgttgcat tatgtctcaa gaagaatgaa
    19201 atatgtcgtg ctttacttaa atgaagagac attggaagaa acagaagaac gaatccgtaa
    19261 attacatttt gttcgaaacg tagaacgttc ttaccgaccg gatatcgaaa tgaattttgc
    19321 cgaaaaaata ggaaacaaaa cagtattaaa agacgaaggt ttcgaaattg aagagctaaa
    19381 tacagaaatc cgattagctg aacatcttta aaaatctctt atgagcagtt tttaaataaa
    19441 aaataacttg gataacgatc gatttttgat cattgtccaa gtattttttt ggtatactag
    19501 taccagtacc atttctaagg agtgagtaga tgcggataat atcaggaaat tatggcgggc
    19561 gccgtttaaa aagtttagca ggggcgaaca ccagaccaac tacagataaa gtaaaagaat
    19621 ccatattcaa tatgattgga ccatattttg atggagagac agtattggat ctgtttgcag
    19681 gaagcggtgg tttagctatc gaagcagtat ctagaggctg cagtcatgcg gtatgcgtag
    19741 acaaaaatta ccaagcgcta aaaatcatca aagaaaacat cgaaattacg aaagaaccag
    19801 agaaatttac cacaataaaa cttgatgcgg acaaagcaat cgaacggcta gcagcggaga
    19861 aacaacaatt cgactacttg tttcttgatc cgccatatgc aaaacaaaaa ataatcgatc
    19921 aaatccataa aatggaagaa ctgggacttt tttctaaaac cgcagttatc gtatgcgaaa
    19981 cggataaaac agtcgaattg ccagaaactg tggctggatt ccatcaaata agaaaacaga
    20041 tttatggcat aacggccgta actatctata gaaaagaggg aaatcaatga aacgtgcatt
    20101 attcccagga agttttgatc cttttacaaa aggtcactta gatacggtgg aacgtgcggc
    20161 taaattattc gatgaggtag taattggtgt ttttatcaat acttcaaaga aaagtttgtt
    20221 tcccccagaa gaaagaatga cattgatcac aaaagctgtt tctcatcttc ctaatgtaaa
    20281 agtcatgcat caagaaaatc aattgacggt agaaacagca aaagagattg gtgcagatgc
    20341 gttgatcaga ggcatacgaa gtatcaaaga ctttgaatat gaacgagaga ttgctcagat
    20401 gaatcatcat ctatatgatg aattggaaac tgtcttctta ttagcaaaac cagaatatag
    20461 tcacgtgagt tccagtattt taaaagaagt actgcacttt ggcggagatg tgagcagcta
    20521 cttaccgcca gtgatcaacg aagctttagc tggaaagcgt gaatcaaatg aaagctaaac
    20581 aatggttgaa aacagtactg attatcatat ttggaatgtt gatcattgga tttgtagtag
    20641 ttcccttgcc gtattatgta gaatcaccgg gagccacgat cgatctgaaa gaactaatta
    20701 cggtcaatga taaggaagat aaaggttctg ggtctttctc tcttacctct gttggtatca
    20761 ggcaagctac aggtttctta gctttaaaag ctaaaatgac cccttttgaa gaattgatca
    20821 gtgaagaaga attgactggt ggggcgacaa atgaagaata taatcaaatg caacagtatt
    20881 atatggattc ttcccaaaat gcagcaatcg aacaagcgtt aaaacttgca gacaagcctt
    20941 ataaaatgga attcaaaggc gtatatgtca tgagtgtaga gaaaacatcg aatttctttg
    21001 gcaagctttc agttggagat acagtaacaa aagtagacgg gaaaagcttt caaagtacag
    21061 aagcatttat ggattatgta aaaagccaaa aagttggaca aacaatcgaa atcagttatt
    21121 tgcataatgg tgaagaaaaa acagcttctg gtgatctgat cgagttacca acagataaaa
    21181 aggcaggaat aggtatcacg cttactgacc ataccgagat cgaatcagat acagatgttc
    21241 gtattaattc tggctctatc ggtgggccat cagcgggatt gatgttcact ttagaaatct
    21301 atgaacaagt cactggtaaa aacttgcgac atggtaagca aattgcagga actggaacga
    21361 tcaacagtga aggagaagtt ggccgaatcg gaggaatcga taaaaaagtg gcaagtgcgg
    21421 ataaagctgg cgtagagatt ttctttgcac cagatgatac gatttcagac gatgttaaga
    21481 aagaatatcc tgaaataaaa accaactacg aagaagcaaa agctgccgct aaaaaattga
    21541 attcctcaat gaaaatcgta ccagtaaaaa cagtacaaga agcgcttgac tatctagaga
    21601 aaatgaaatg aaactaaat
//
LOCUS       DAYYME010000040        20656 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000040 DAYYME010000000
VERSION     DAYYME010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.040"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..405
                     /locus_tag="ACHBFW_001240"
     CDS             37..405
                     /locus_tag="ACHBFW_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFM9317553.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            575..1006
                     /gene="mraZ"
                     /locus_tag="ACHBFW_001241"
     CDS             575..1006
                     /gene="mraZ"
                     /locus_tag="ACHBFW_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFM9317554.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            1027..1986
                     /gene="rsmH"
                     /locus_tag="ACHBFW_001242"
     CDS             1027..1986
                     /gene="rsmH"
                     /locus_tag="ACHBFW_001242"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFM9317555.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            1991..2392
                     /gene="ftsL"
                     /locus_tag="ACHBFW_001243"
     CDS             1991..2392
                     /gene="ftsL"
                     /locus_tag="ACHBFW_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFM9317556.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            2393..4585
                     /locus_tag="ACHBFW_001244"
     CDS             2393..4585
                     /locus_tag="ACHBFW_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM9317557.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            4611..5573
                     /gene="mraY"
                     /locus_tag="ACHBFW_001245"
     CDS             4611..5573
                     /gene="mraY"
                     /locus_tag="ACHBFW_001245"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFM9317558.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            5587..6948
                     /gene="murD"
                     /locus_tag="ACHBFW_001246"
     CDS             5587..6948
                     /gene="murD"
                     /locus_tag="ACHBFW_001246"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFM9317559.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            6974..8062
                     /gene="murG"
                     /locus_tag="ACHBFW_001247"
     CDS             6974..8062
                     /gene="murG"
                     /locus_tag="ACHBFW_001247"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFM9317560.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            8077..9297
                     /locus_tag="ACHBFW_001248"
     CDS             8077..9297
                     /locus_tag="ACHBFW_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFM9317561.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            9453..10778
                     /gene="ftsA"
                     /locus_tag="ACHBFW_001249"
     CDS             9453..10778
                     /gene="ftsA"
                     /locus_tag="ACHBFW_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFM9317562.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            10801..12042
                     /gene="ftsZ"
                     /locus_tag="ACHBFW_001250"
     CDS             10801..12042
                     /gene="ftsZ"
                     /locus_tag="ACHBFW_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFM9317563.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            12059..12736
                     /locus_tag="ACHBFW_001251"
     CDS             12059..12736
                     /locus_tag="ACHBFW_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFM9317564.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            12750..13349
                     /locus_tag="ACHBFW_001252"
     CDS             12750..13349
                     /locus_tag="ACHBFW_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFM9317565.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            13430..13651
                     /locus_tag="ACHBFW_001253"
     CDS             13430..13651
                     /locus_tag="ACHBFW_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFM9317566.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            13700..14482
                     /locus_tag="ACHBFW_001254"
     CDS             13700..14482
                     /locus_tag="ACHBFW_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFM9317567.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            14503..15210
                     /locus_tag="ACHBFW_001255"
     CDS             14503..15210
                     /locus_tag="ACHBFW_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFM9317568.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            15472..18270
                     /gene="ileS"
                     /locus_tag="ACHBFW_001256"
     CDS             15472..18270
                     /gene="ileS"
                     /locus_tag="ACHBFW_001256"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFM9317569.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(18319..19845)
                     /gene="zwf"
                     /locus_tag="ACHBFW_001257"
     CDS             complement(18319..19845)
                     /gene="zwf"
                     /locus_tag="ACHBFW_001257"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFM9317570.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(19860..20507)
                     /locus_tag="ACHBFW_001258"
     CDS             complement(19860..20507)
                     /locus_tag="ACHBFW_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM9317571.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
BASE COUNT         6983 a         3575 c         4417 g         5681 t
ORIGIN      
        1 atacttgaag caacgataaa aaaggagttt acgttcatgg gcacattcac accacttatc
       61 ttgttttggt atatttaccc ggtaatcgtt ttgtttggtt gccaatttct tgtaaaatta
      121 ttttcgttaa atcgtcgttt taaaataaaa gcccctgatt tggcgattcc ttttttgtgg
      181 ggcggtatgc atgcattgtc tagaaataca gggacaattt ctatcttacc gtttttcttt
      241 atttctgttt tgctaatggg aatttgtatt gctgttttcc aagcatatta ttatgaagaa
      301 attatttatc cgcgttattt caagatgact tggcgtttgg tttttctgtt gacaatcgtc
      361 ttgtacgctg tcttgatcat tgtgaacata ctttcttacc tatgaaaaaa atagaaaagc
      421 tgtaaaaagt cttttaaaag gctttttgca gcttgatttt taaaaaaatg tttatgaata
      481 aaaaaagttg ttcccggatt tcccagaaaa ggtggtagaa agtgggggat tgtggtagac
      541 taattttagt tagtggagga atggggggct ttcaatgttg atgggtgaat tccaacataa
      601 tattgatgca aaaggacgtt taatcgtgcc ctcaaaactt cgtgaagaac ttggcgaaaa
      661 gtttgttctg acacgcggat tggatggctg tcttttcggc tatccgatga gcgaatggga
      721 aaatctggaa gctaaactga acgaaatgcc gttggctaaa aaagacgcac gtacttttgt
      781 gcgattcttc tattcggctg ccactgaatg cgaacttgat aaacaaggac gaatcaatat
      841 acccagcacg ttaagaaatt atgcagcttt gacaaaaggt tgcgtagtca tcggtgtatc
      901 taaccgaatt gaaatttggg atgagacacg atggcaagat ttttctgcag cagcagaaga
      961 gaactttgac gaaattgctg aaagtatgat tgatttcgga ctataagaat aggaagaggc
     1021 tgactaatga ctgaaacatt tcaacactat acagtcatgt tgaaagaaac ggtagatgga
     1081 ttgaacatca aaccggatgg cgtttatgta gactgtacac tcggcggtgc cggacatagt
     1141 gaatatttat taagccaact aagttctaaa gggcatctgt atgcgtttga ccaagatcaa
     1201 aaagcaatcg atcacgccaa gctgcgttta gcttcttatg tcgaacaagg tatggtcaca
     1261 tttatcaaag cgaacttcag agaattagaa gaacgcttga gtgagcatgt agataaggta
     1321 gacggcattt tgtatgatct aggggtttcc tctcctcagc tagatgaagc agaacgtgga
     1381 tttagttatc atcaagatgc accgttggat atgcgcatgg atcagtcagc tcctttatcc
     1441 gcttatcacg taattaatga atattcctac catgaattag taaagatttt cttccgctat
     1501 ggcgaggaga aattttccaa acaaattgct cgtgaaatcg aacgagtacg aaaaacaaaa
     1561 ccaatccaaa caactggtga gcttgtcgaa atcattaaac aagtcatccc agctcccgca
     1621 agaagaaaag gcgggcatcc tgcaaaaaga atttttcaag ctgtgcggat tgcagtaaat
     1681 gacgaactcg gtgccgaaga ggcctcttta gaacaggcaa tccggttatt gaaagtcaat
     1741 ggaagaatca gcgtaatcac attccattca ttggaagaca gaattgtcaa aagtatgttt
     1801 aaagaatata gtacggtaca agatctacca ccgggacttc ctgtcatacc tgacgaattc
     1861 caaccagaac tgaaagtcat tactcgtaaa ccaatccttc cttcagagga agagctaaca
     1921 gaaaacaatc gatcacgcag tgcaaagtta cgtattgcag aaaaaataag aatgaaagag
     1981 gagtagaatg atggcagaat taaagaaaat gcaagattac gaatatgatt tacctgaatt
     2041 ggtggatcag ccggaacaac aaatgcctga ttcttcttcg aatcaagaaa tcattcttcc
     2101 acaatcaccg gcaaaacgat taaagcagat ttctgctttg gaaaaaattg ctgtggcaag
     2161 cgtggcgatt gctattgttg ctttatgtgt tttaacagtt atgctgcgca caaatatcag
     2221 cggagctgaa aaagaaatca cgtcaataca aacagaaatc aatgataaaa agcaagaaaa
     2281 aacgagttta gaacaagaaa aaaacgaact ttctcgtact gaacggatta aacaaatcgc
     2341 agaagaaaaa ggattgtcta ttaatgacga caatttaagg aaagtgaagt aaatgagtct
     2401 taaaaataaa attcggcggt ttatggaaaa gaaaaatcta aatccgatga acaatcgcaa
     2461 aaaagtaggt atcattttat ttgctacgag tattggattg ttctttttat ttgccgtccg
     2521 tttctcgtat atcgttattg gaggtcatgt tgctggtacc tcattagctg aaaaaacaaa
     2581 acagttgtac caaggaagtg aagtagtaaa agcaaaaaga ggaacgattt atgatcgtaa
     2641 cggtgtggcg ctcgcagaag atgcctcctc ttattcaatt aaagcgatac tctcaaaaac
     2701 atatacctct ggggataaaa aactgtatgt tgaagaaaag aattttgata aaatagcaga
     2761 aatcctgcat aagaatcttt ctatagacaa aaaagatgca ctgaatattt tagaggacgg
     2821 cgcaaaaaaa gagctgtatc aagttgaatt tggtagctat gggaaaaata tctcccaaga
     2881 aaccaagcaa aacatcgaag cggacatgaa aaaagaaggt gtcgctggtc tttattttgt
     2941 cgaccatcag gctcggatgt atccaaatgg cgtcttttct tctcatttta ttggatacgc
     3001 agttccagac aaagatgaga atggcttagt tggtaaacta ggtttggagt ctgcttacaa
     3061 tgatatcttg agcggtaaag atggtaagat tatttatcaa aaagataatt ttcaaaatcc
     3121 tctgccaggt acagtggcag aagaagaaaa agctgtcgac ggacaagata tctacacgac
     3181 tttagacagc agactacaaa gttatttaga aacactgatg gatcaagtaa atgaagaata
     3241 tcagccagaa gaattgacag cagtcctgat gaaagcaaaa accggagaaa tcttggcaat
     3301 gggacaacga cctactttca atcctgaaac aatggaagga ttgactggaa aagatgctat
     3361 ctggcgtaat ttcttggtac aagatagtta tgaaccagga tcaaccatga aagttttcac
     3421 tactgcagct gctatcgaag aaggagaatt caacgagaat gaaaccttcc aatctggaaa
     3481 aatccaagta gaagatgcta cgatcaatga ccatgacttt ggtgaaaaag gtgtgctgac
     3541 catgcgtcaa gctctttcat ggtcaagtaa cgtcgggatg gtcatactgg aacagcgatt
     3601 aggcggacga tggtataact atttacaaaa actgggattt ggacaaagta ctcattctgg
     3661 attagatgat gaagtaaacg gtgctttacc tacattaaat attgtcgacc gagcaatgag
     3721 tgcttatgga caagcggtag gtgtgacgaa tttccaaatg atgaaagctt tcacttctat
     3781 tgcgaataac ggtacaatga ttcaacctcg atatatcagc aaagtggtgg atccccaaac
     3841 tggagaagag cgaacaactc aaacagaagt tctagggcaa cctttttcaa aagaaacaac
     3901 agaaaaagtc cgtgaatata tgagagatgt tgtcgaaagc gaaaactacg gtagtgctta
     3961 tggtgtatat agtgtacctg gatacaatgt atcggcaaaa aacggaacag cccaaatcgc
     4021 ttctgataca gggggatatc aaacaggaga tacagcctac ctgtattcga ttgtggagat
     4081 ggttccttct gaagatccag actatgtctt gtatctaaca atgaaacacc ctaagacata
     4141 tgatcgaatg gcattggcta aaatcgcaaa tccattgatg aaacgggcaa tggattttaa
     4201 agaaactgaa gaagattcag atacagaaac aaaaacagaa aaagtatccg tagccgatta
     4261 tcgtaatctt gaagcggacg ttgcagcagc tgatgctcaa aaaagtggac tgcaacctgt
     4321 agtgatcggt aatggtaaaa aagtacaaaa acaatctact gccaacggtg atcagttgat
     4381 ctctggagaa aaactgatcc tatatactgg tggtgataag ctgatgcctg acgtaacagg
     4441 atggtcaaaa gcagatatca tgaagctggg gaaaatcctc ggtattgaag ttagttttga
     4501 tggagatggt tattgtgtca aacaagaact tgctccatat gaaaaaatca ctgaagacaa
     4561 acttagtttt actttagaag aataagaaga gaaataggag agaaaagagc atggaatgga
     4621 cacaagcatt gattcccatt gtcagcagtt gtgcaatgac aattgccgca atgccgctat
     4681 ttattggtta ttttcaaatg aaaaagcaag gtcaggcgat tcgtgaggag ggaccaaagt
     4741 ggcacaatag caaagccgga actcctacaa tgggcggctt agtgtttctg atcggctcta
     4801 ttttgacagg aatttgggtt ggagcttggc aaaaacagct aactcctact cttttcatct
     4861 tgctttttgt attagcgtta tacggagtaa tcggctttct tgatgatttt ataaaaatat
     4921 tcaagaaaag aaacatggga ctgaactcga agcagaaatt gattggacag attctagggg
     4981 gaatcatctt ttatcttgtt taccgttctg aaggttatcc aggtgtattg aatttcttcg
     5041 gactagatct acctttagga atcatttatg gtattttcgc tctcttctgg cttgttggat
     5101 tttcaaatgc agtaaatctg acagatggca tcgatggatt agttgctggc ttaggaagta
     5161 tctcttttgc agcttacggt ctaattgctt ggcatcagca tcagtatgat gtattaatca
     5221 tttgcttaag tgttttagga ggtttactgg gtttcttcgt ctacaacaga aaacctgcca
     5281 agatttttat gggagatgtc ggttcattag cgttgggcgg acttttagcg gcaatttcta
     5341 taatgctaaa ccaagaatgg acattgcttt tgattggttt gatctatgtt atggaaacag
     5401 caagtgtgat gcttcaagtt acttccttta agcttacagg aaaacgaatt tttaaaatgt
     5461 ctccaattca tcatcacttt gaaatgtgcg gatggtcgga gtggaagatt gatacagttt
     5521 tttggttgat cagcattgtg acttcgttga tcacattatg gatcgtttgg taggagatga
     5581 cgggaaatga aaaaaatatc aacatatgaa aataagaaag tgcttgtctt aggactcgca
     5641 aaaagcggag taagtgcggc caaattatta catgaattgg gcgcacttgt caccgttaat
     5701 gatggaaaac cttttgacga aaatccggaa gcacaagagc tgctttcttt agggataaaa
     5761 gtaataacag gtagtcatcc aatcgaattg ttggatgaag aattttcatt gatggtcaaa
     5821 aatccaggga ttccctacag tcatccttta gtagcaaaag cccaagaaat gggtattccg
     5881 gtaattacgg aagttgagct agcttacgaa gtagcggaat gcccaattat tgggattaca
     5941 ggaacgaacg gaaaaacaac gacaacaacg atgacaggac tactactgaa tgcaggagca
     6001 gatcaaggaa ttgcacgttt agcgggtaat attgggtatc cagcaagtgg tgtagcgcaa
     6061 gaagcaaaat cagaagataa aattgtgatg gaattgtcta gcttccaact catgggaatc
     6121 acagatttcc gccctcatat agcggtgatt accaatatct atgaagcaca tattgattat
     6181 catggtacaa gaaaagaata tgtcaaagct aaatggaacc ttcaaaaaaa catgacagaa
     6241 aaagattatc tgatccttaa ctggaatcag tcagaactac aagaattagc gcaaagaaca
     6301 aaagcacgcg tactgccttt ctcgacaaaa gaagtgttgg aagacggtgt gtatgctgac
     6361 gattactcaa tatactataa aaaagaaaaa ataatggaaa tatctgaact tggcgtacca
     6421 ggtaagcata atgtagaaaa tgcactagct gctatttctg tagcaaaatt gtatggtata
     6481 tcaaatgaag ctatacgaga aacgttgcat tttttccatg gcgtaccaca tcgtacgcaa
     6541 tatgtaggag aaatccaagg cagaaaattt tataatgatt caaaagcaac caatatttta
     6601 gcgactaaaa tggctctaag tggatttgaa acgtctaagg tcgtgttatt ggctggcgga
     6661 ttagacagag gaaatacatt tgatgaatta attccttcat taaaaggaat caaagcaatg
     6721 gtcgtatttg gccaaacaaa agaaaagttg atggatgctg gaaaaaaagc aggcatcgag
     6781 acaatagtaa cagccgattc tgtagaacaa gcagttcctt tagcattaga aaacagtaca
     6841 gatggtgatg tagttttatt atcaccagcg aatgccagct gggatcaata cccaaatttt
     6901 gaaactagag gaaacagatt catggaagct gtgaaccgat taaaatagaa aaagtagaag
     6961 cgagcgataa aaaatgaaaa ttttggttac aggcggagga acagggggac atatttatcc
     7021 agctcttgcc tttgtcaatt atgtaaagac tcaagaaccg aatgctgaat tcatgtatgt
     7081 gggagcaaaa cgaggtctag aaaacaaaat cgtccctgat acaggaatgc ctttccatac
     7141 cttggagatt caaggattta aacgaaaaat ttccatgcat aacatcaaga cggtccgatt
     7201 gtttcttaag agtatccgtc aagcaaaaaa aatcctcagt gattttcaac cagatatcgt
     7261 tatcggtact ggaggatatg tttccggagc ggtagtgtac gctgcagcca aaatgggtat
     7321 tccaacagtt atccatgaac aaaacagtgt accaggtgtt accaataaat ttttaagccg
     7381 atatgtggat cggatcgctc tttcatttaa agatgcggct tccttttttc ctgaaaacaa
     7441 agcagttctt gtaggtaatc ctcgagcaca agaagtagca gatactaaaa aatcagatat
     7501 tttgaaaaca tttgatttgg atccagaaaa gaaaacagtc ttgatttttg gcggtagtca
     7561 aggagcactt aaaatcaatc aagcagtatc agttttttta agtgaatata ttccttcttc
     7621 ttaccaagtt ttgtatgctt caggagatag atattatgaa gagatcctaa ataaaataaa
     7681 tcctgaaata caaaatatta gtgtccagcc ttatatcaaa aatatggcag aggtcatggc
     7741 ttccagtgat ctgctggtgg ggcgtgctgg tgcaacttcg atcgcagaat tcacagctct
     7801 ggggttaccg gcagtgttgg ttcccagccc atatgttacg aatgatcatc agactaaaaa
     7861 tgcaatgagt cttgttcacg caggagcagc aaaaatgatt gcagataatg aactaacagg
     7921 tgaatcacta agtcagacag tcaacgaaat catgggtgat gaagaattgc aaaagcaaat
     7981 gtgccgagct tctaaagaac aaggaatccc tgatgcttca aaacgtcttt atgacttagt
     8041 aaaacaaata atcaaaaaat aaaggcggtg gtcttagtga gtaagaaaaa agaaccaaaa
     8101 aaattgtctc atccgtcttc tgatgaaaac aatttgactc cttggcaaaa ggctaaccga
     8161 aaatatctgg cagaacaaga gatgaaaaac caagagccaa aaaaagaaga cataacatta
     8221 gatacacccc tttcagattc tgaagaagaa aaaacggttc aagaggaaca gcaagaagaa
     8281 agccagtccg agaaaaaaat cgaatttttt gaagagatca agcaaaagaa aaacggtgga
     8341 ccatataacg gttctttttt gaaccggctg cccaatttaa aaagtcaacg gcacaaagta
     8401 ctatttcgtc ggttagcttt gatcatcact gtattgggta taccgctgat ttttttgatc
     8461 tattatgttt ctccttatag caaattacaa gcagttgcag tatcaggcaa taagacagtc
     8521 aacagccaag aaatcatctc tgatacaaaa ttgtcattag gagaaaatgt ttggggacag
     8581 tattttcatc gatctactta tatcgatcgc cttaaaaaag cacagccaag aatcgaaaca
     8641 gcaaatatac atttcaaagg gatgaatgaa ttcgatttgg atgtaacaga atataaagag
     8701 attgctttga tcgcaaaaaa caatcaatac tacccagtga ttgaaaatgg aacagtatta
     8761 gatgaaaaag tagcaaatcc tacaaaaaat ctccctatct tggaagagtt caaagactct
     8821 gcaaaaatca aagaattaac aaagcaatac aatcagttat catcagaatt acaaaaagca
     8881 atttctgaaa tcaagtacac gccaagagca agcaataaaa atctgatcca attgaatatg
     8941 aatgacggca atcaagttat tgtaaatatc aataatcttg cgaaccaaat gaaatattat
     9001 tcgcaagtag ccaaagatat ggatgaaaaa ggtgtgatcg acatggaagt tggtatcttc
     9061 tcgcatcctt acggtagcac tcaagaaacg gaagaatcgg gccaatcgga agaaacaact
     9121 gaaagtgaag aaaatgcgga tcaaacaaac gaatcaagtg aagaggcaga aacgcaaaaa
     9181 gcacaaacac ctggatcgtc tagcacaaac ggatcatctg attcaaatga ttcaagtgaa
     9241 acaagtcaaa tagaagataa tccaagtaca actccgtcaa gtttaaatga aaattgataa
     9301 aacaacttta aaaagtaaga atgtactttc taaaaaccag taatttatgg tagaattgga
     9361 agcagtgaat atatctatta ataagtatat tttaaaacgg acgctagttg ttcgcttgta
     9421 tatggaatag taagtaggag ggaatcccat tcatggcaaa aacaggaatg tatgtaggcc
     9481 ttgatattgg aacgacatct gttaaagttg tcgtggctga atatatcgac agccaaatga
     9541 atattattgg agtaggaaac gcaaaatcag aagggattaa ccgaggcatt atcgttgata
     9601 tcgataaaac ggtccaagcg atacaacgag cagtacgaca agcagaagaa aaagcaggta
     9661 tccagatcaa aggcgtaagt gtcggtttgc cagcaaacat gcttgaagta gaaaattgcc
     9721 agggaatgat tgcagttaac ggagattcaa aggaaatcac cgatgaagat gtcagaaatg
     9781 tggcttcagc tgcccttgtt cgttcaatac ctcctgaacg tcaaatcgtg tcaatattgc
     9841 cacaagattt tactgtcgat gggttcgagg gaatcaaaga tcctcgtgga atgatcggcg
     9901 taagattaga aatgtacggt ctgttgttta caggacctaa aacgatcatc cataacatac
     9961 gtaaatgcgt ggaaaatgcc ggattaatcg tcaacgaaat ggtgattact cctttagcgc
    10021 tgactgaatc aattctttct gacggagaaa aagattttgg tacgatcgtc attgatatgg
    10081 gtggcggcca aacaacaacg gctgttatgc atgataagca attgaagttt accaatcttg
    10141 accaagaagg tggcgaattt gtcactaaag atatctctat tgtattaaat acgtcattta
    10201 acaatgcaga agcgttgaaa ataaactacg gggatgcata cccagaacgg acatcaccag
    10261 atgaagaatt tccagtagat gtgattggtc agtcagaacc agtgaaagtc gatgaacgct
    10321 acttgtcaga aattatttca gctcgaatgg aacagatttt caacaaagca aaagatgcat
    10381 tggaccaaat cgaagcatta gagttgccag gtggcgtcgt attgacaggc ggagctgcta
    10441 gtcttcctgg tgttgtggac ttggcacaag aaatcttcgg cgtaaatgta aaattgtatg
    10501 ttccaaatca catggggctg agaaacccgg tatttacaaa tgtaataagt atcgtggatt
    10561 attcagcaaa cttgagtgaa gtgtatcaat tggcaaaaat tgctgttacg ggtgaaacct
    10621 cagcagctcg tcaaatggta gttgaacaag aaacaacaaa cacatatgag agctatgaag
    10681 cacctgaaga aacaatctac gatgagccgg aaccaaaaga gtccggagaa aatgtaaaaa
    10741 ataaaatcaa gggtttcttt acaaatattt ttgattaatt catcctggga ggaataagat
    10801 atggaattct caattgataa taatatcaat gacggcgcag taatcaaagt catcggagtc
    10861 ggtggtggcg gtggaaatgc tgtaaaccga atgattgaag aaaacgttaa aggtgttgaa
    10921 ttcattacag ccaacacaga cgtgcaagca ttaaaaaatt caaaagctga aacagttatc
    10981 caattaggcc ccaaatatac tcgtggttta ggtgctggtt ctcagccaga agttggacaa
    11041 aaagcggcag aagaaagtga acaatcctta cgcgaagctt tagacggtgc agatatgatt
    11101 ttcatcactg caggaatggg tggaggaacc ggtacaggtg ctgcaccaat cgttgcaggt
    11161 atcgcgaaag aattgggcgc attgactgtt ggtgtcgtga cacgtccatt cacttttgaa
    11221 ggaccgaaac gtggccgttt tgctgcagaa ggtatcgctc gtttgaaaga aaatgtggat
    11281 acattattaa ttatctcaaa taaccgttta ttagaagttg tagataaaaa gacaccgatg
    11341 cttgaagctt tccgtgaagc agataatgtt ttacgacaag gtgtacaagg tatctctgat
    11401 ttgatcacag cgccaggtta cgttaacctt gactttgctg acgtgaaaac agtgatggaa
    11461 aaccaaggta cagcattgat ggggatcggt gtggctagtg gagaagatcg cgtagtagaa
    11521 gcaacgaaaa aagcaatctc ctctccattg cttgaaacat ctattgatgg agctgaacaa
    11581 gtacttctta acatcacagg cggtttggat atgacattgt tcgaagcaca agatgcttca
    11641 gatatcgtag caaatgcagc aactggcgat gtaaatatca ttctaggtac atctatcaat
    11701 gaagaaatgg gcgatgaaat ccgtgtgacg gtcatcgcaa caggtatcga tgaatcaaaa
    11761 aaggaacgca agtctagccg accagctaga caagcacaaa tgcaatcacc tgctcaaaag
    11821 actgtattag atatggatca agctaaacca acttcttcag aagaagaaaa cagttttggc
    11881 gattgggata tcagacgtga acaaaatgtt cgtcctagag ttgatgattc caattttgac
    11941 aatatcgaaa aaaaagaatt tgatacattc aaccgcgaag aaacaaaatc tcatggcgat
    12001 gatgaattaa atacgccacc gtttttccgt cgtaaaaaat aaaggagagg aagagcacat
    12061 gattgctgac aacttgcaga aaatcaagca agaaattcag gattcgtgtg ctcttgttca
    12121 tcgtgatccg aaagaagtta cattagtagc tgtaacaaaa tctgttgaat ccgctcaagc
    12181 tgaagaattg gttagagctg gcgttatgaa ttgtgcagaa aaccgagtag ataaattatt
    12241 agaaaagaaa caagatttat ttgattttcc tgaaataaag tggcatttaa ttggtaattt
    12301 acagcgcaga aaggtaaaat tgattgtaaa tgaaattgat tactttcatg cgttggacag
    12361 tttaagatta gcagaagaaa tccagaagcg gttagagaaa gaattatcct gttttattga
    12421 agtaaatgtt tctggtgagg aatcaaaaca tggaatttca ccagatgagc ttttaccgtt
    12481 tgtggaatct ttagctgcct gcacaaaaat caaggttgtt gggttgatga ctatggcgcc
    12541 taaagaagct tctgatgagc aaatccataa agtgtttggt acgctgaaag aattgcgtga
    12601 tcttgttcaa gcaaaaggtt atgcccatgc accatgtagt gaattaagta tggggatgag
    12661 tcaagacttt caaattgcga ttgaagaagg tgcaacattt atccgtgtag gaacagcgct
    12721 attcaaagac gaataaagga ggaacaaaaa tgccgctttt aaacagagga gcaattgcta
    12781 attttttcgg attagctgat gaggaagagt acgaatacca agattatcct gaaacaacaa
    12841 aaaaggctgt acagccaact aaaaaaaata caacacagaa tcaatcaact cattctgtat
    12901 ctgctgcgaa tactcatgaa aaacaaaaaa cggcgacttc acgtcctgta acgcatgaaa
    12961 gtattcagcc ggctgaaaag aaaaaagtaa ttgaactaca tcaatcacct acaaaaactg
    13021 tagcgaaaga aagaacagct tcaagaacct ctacacaacc taaagcaaat cttgctggaa
    13081 aaatcactgt aatggaacca agagcttatt ctgaagctat ggcgattgct aaacgaatca
    13141 ttgctggaga agcaacctta gtgaattttc atttagtaga agaaaaacaa gcgagaagaa
    13201 tcgttgattt tctaacagga acagtttatg cactagatgg agatatcaaa cgtgttggag
    13261 atgagatttt tctttgtaca ccgactggga tggagattga cagttcaacc gctcaatctt
    13321 ttgcagatgc gaatttgttt gatctgtaag aggaggaact acgatttata ctattgcact
    13381 attacttcat ttacttcaac gggcagccta tttctatagc attatcttga tggtatatgc
    13441 attgctctct tggtttccag gcgggtatca atcagggcta ggacgattat tagctaagat
    13501 ctgcgaaccg tatatcagtt tgttcgatcg tctgcctttg aggattggac ccttggattt
    13561 cacaatttta ggggcaatcc tgatcttgaa cttagccgta caaggtttag cacagatcgt
    13621 tatatggata actgtccatt tagtttattg attgaagcaa caaaatcttt attctcagaa
    13681 tagataggag gggaaaattt tggatgcaaa tgtataccag cactttcggg cagatgaacg
    13741 tacatttatc gattcggtag gcgattggat tgaacaggtt caaggacaat atgcacccta
    13801 tctgaccgaa tttcttgacc caagacaatc ctatatttta gaaacactga ttcgacaaag
    13861 cagtgatttg cgtttcatgt tttatggcgg atatgaacag gcagaaagaa aaagatgttt
    13921 gattttcccc gattattacg aacctgtaga ggaagatttt gaaacagcat tgtatgaaat
    13981 ccattatcct agcaaatttg ccagcctaag tcatgggaaa atccttggaa ctttagtggg
    14041 aacggggatc aaacgctctt actttggcga tatcatctcg gacggtgatc gctggcaagt
    14101 atttattgca aatgaggtgg atcattttgt tgctgcacaa gtaacaaaga ttggaaaggt
    14161 tgctgtacgc ttggaaccaa tcaaatacac agaaatgatt atgccgaaag atagctggac
    14221 acaagaaaga ggaacagtta gttccatgcg actagacagt gtgatctctg agatttataa
    14281 tatttccaga cagcggtcca agcagctgat cgaagctggt aaagtaaaag tgaactggac
    14341 agaaaataca agacctgatt ttgtacttga actattagat attatttcag tcagaggtta
    14401 tggcagagtc cagatccaag aacttgaggg gaagacgaaa aaagaaaagt acagggtact
    14461 gttcggtgta ttacgtaaat aaaataaaaa ggtggtttac tcatggcttt aactccatta
    14521 gatatccaaa ataagacatt cccgacaaaa atgcggggat ataatcaaga cgaagtagat
    14581 gatttcttag atttagttgt tcgtgactat gaggaattga ctcaaagaaa tcgagaatta
    14641 gaaaaagcag tcaaacattc tgaagaaaaa cttgagtact tcaatgaatt aaaagatgct
    14701 ttgaaccaat caatcatcgt agctcaagat acggctgata aagtaaaaac aagtgcaagc
    14761 aaggaatccg aagtgatcgt tacttctgca caaaacaaag cagatgagtt agttgcgaac
    14821 gctgaaaaac gtgcacatca attaacaact gatgcagaag aaaaagcaag aaaaatcttg
    14881 acagatgcaa cagaaaaagc aagacagctt gcgacagaaa ctgaagatct taagaaaaaa
    14941 acacgagtat tccatcaacg tatcagcttg atgcttgagt ctcaattaga acaagtgaaa
    15001 agtccggaat gggatgaaat tttacaacca ttctccagct acgtgactga cagccatgaa
    15061 gtgatcaaag aagttttagc aaaacagctt gacaatgaaa atgatactga tgtaaactca
    15121 gacacaggag tcatagaagc acctatgacg gcatttgata cacaggctat tgatatctcg
    15181 atcgtgcctg gtgaaaatga aaacgagtag aacagaaaag cactttgtta ttcttaaagc
    15241 gagccgatga gagtggaagt tcggtaagac actgcatggt gaaagatcct ctacttgaga
    15301 cctgcttttc cgaaattaga gtaagaagca gcggctgacc tcgttattgg tcacagagag
    15361 tttgcactag atagatgcaa ctaaatttgg gtggtaccac ggcatgttcg tcccttgcgg
    15421 ctgaacatgc ttttttgtgt ccggaaaaca aataaaggag tgtatggttg aatgaaaatg
    15481 aaagaaacct tgcaattagg aaaaacaaaa ttccctatgc gtggtaattt gccaaaccga
    15541 gaaggcgagt ggcaaaaaga atgggaagaa aatcgaatct accaaaaaag acaagagttg
    15601 aacgaaggaa aacctagctt tgttcttcat gatggacctc catatgccaa tggtaatatc
    15661 catttaggac attcattgaa taaaatcagt aaagacatta tcattcgttc aaaatcaatg
    15721 tctggctatc gtgcaccata tgttccagga tgggatacac atggattgcc gattgaacaa
    15781 gtattggcta ataaaggcgt gaaacgtaaa gaaatgtcca tggcagaata cttggaaaaa
    15841 tgtcgtgaat atgcactttc ccaagtagat aagcaacgag cagatttcaa acgcctagga
    15901 gttgcaggag attgggaaca tccttatgtt acattagatc cttcttatga agctgcacaa
    15961 gtacgcgtat ttggtaaaat ggctgaaaaa ggctatattt acaaaggatt gaaaccaatt
    16021 tactggtctc cgtcaagtga gtcttcttta gcggaagctg aaattgaata taaagatgta
    16081 aaatctccat ctatttttgt agctttcaat gtcaaagacg gcaaagatat tcttgatgaa
    16141 gatacagctt ttgtcatttg gacgacaaca ccatggacat tgccagcaaa ccaaggtatt
    16201 gctgtaaatc caaaatttac gtatgtattg gtggaagccg atggccgtaa gtttgtcgta
    16261 gcgaaagatt tgctagaaac agtccaatca gaaatcggtt gggaaaatgt aacagttcta
    16321 aaagaatttg ctggacaaga aatggaatat atgactgcaa gtcatccatt ctatgaacgt
    16381 gaatcacttg tgattttagg cgatcatgtt actttagacg ctggtactgg attagtccat
    16441 actgctccag gacatgggga agatgactat atcgctggta gcaaatataa actatctgta
    16501 gtgtcacctg tagactcaaa aggattgttt acagaagaag cacctggatt tgaaggtatc
    16561 ttttatgaca aagcaaatcc tatgatcact gatctattaa aagaaaaagg agctttgctg
    16621 aagctagatt tctttactca tagttatcct cacgattggc ggacgaaaaa gcctgtgatc
    16681 tatcgggcaa ctccacaatg gtttgcatct atcgataaat tccgtcagaa tattttggat
    16741 gaagtagaaa aagtagagtg ggttatccca tggggtaaaa cacgtttgta taatatgatc
    16801 cgtgaccgcg gcgattgggt aatttcccgt cagcgtgcat ggggagtacc acttccgatt
    16861 ttctatgctg aaaatggaga agcaatcatc acaccagaaa caatcgaaca tgtggcaaaa
    16921 ttatttgctg aacatggatc aattatctgg tttgaaaaag aagcgaaaga acttcttcct
    16981 gaaggattta cccacccagg ttctcctaat ggcgaattta caaaagaaaa agatatcatg
    17041 gatgtctggt ttgattctgg ttcttctcat gaagctgttt tacgtcagcg accagaatta
    17101 tcattcccag cagatatgta tttagaagga tcagaccaat atcgtggctg gttcaactca
    17161 agtatcacga ctagtgtggc tatcaatggt gtagcaccat acaaatcagt tatttctcaa
    17221 ggatttgctt tagatggtga aggccgtaag atgagtaaat ctttaggaaa catcattgca
    17281 ccagaaaaag tgatcaaaca atttggtgca gatatccttc gcttatgggt atctagtgta
    17341 gattatgaag cagatgttcg tgtttctatg gatatcttaa gtcaagtggc agaagtatat
    17401 cgtaaaatca gaaatacgat gcgtttctta ttagcgaata cagaagattt cgatcctaaa
    17461 aaagatactg tttcgtacaa tgatttacgt tctgtagaca aatatatgac cgttcgattg
    17521 aaccaagtaa tcaaagaaat ccgagaagaa gggtacgaca aatataactt catgcatatt
    17581 taccgtacag ttatgaactt cttgacggtt gatctatcgt cattctacct ggattttgct
    17641 aaagatgtag tttatattga agcagaagat gattatcaac gtcgttgcat gcaaactgtt
    17701 ttctatcaaa cagctgtagc tttgacaaaa ttattgactc ctatcattcc gcatacagca
    17761 gaagaaatct ggtcattcct gaaagaagaa gaggagtacg ttcagttaag cgagtttcct
    17821 ggttatgaag agtttgcaaa ccaagaagaa ttgatggata cctggactgc tttcatggac
    17881 ttccgtgata atgtcttaaa agcattggaa gaagcaagaa acagcaaatt gattggtaaa
    17941 tctttggaag caaaactaac tgtttatcca aaacaacaag ttcgtgaaat gttaaaagct
    18001 ttggatgcag atattgcaca attattgatc gtttcaccag actactttga agttatggca
    18061 gcagatgaac aagtgccaga taacgctatg gtatttgatg atgttgctat tacagtggaa
    18121 aaagcagacg gggaaacttg cgatcgctgt cgccaagttc gtaaagatgt aggagtagat
    18181 gaaaaacttc ctcatctatg cggtcgttgt gcaaaaatcg ttgaagactt ctatcctgaa
    18241 gctgtagctg aaggattcga agaaaagtaa aagggaaaag ggggtgtgac atgttctttg
    18301 tcacaccccc ttttgttatc aatcgtttaa ttttccacgt tcacgatacc agttatctgg
    18361 ttgccagatc cattcgaatc cgtctttttc aagtagttcg aaagctgctt taggtcccat
    18421 tgatccagct ttgtagttgg gaaaatccgg ttgtgtttgg tcccaagctt ttcgaatgac
    18481 atccacgata tgccaagatt gagcgacttc ttcccaatga gagaagttcg ttccatcacc
    18541 attcaacgca tctaatagta atttttcgta agcttctggg ctattttcaa ccatttctgc
    18601 actatttcta aattccaatt tgattggttc cgtttcaaat ccttgcccta cttctttccc
    18661 attcaaggat aaagaaaatc cttcagttgg ctgaatatag atcgttagaa cattaggtgg
    18721 aagtgtcgta tcgtcacatg gttcatcgac agatgttttg aatacattaa caggaacttg
    18781 tttgaatacg atgttgatcc gagtcccttt ttcagtcaaa cgtttgcctg tacgtacata
    18841 gaaaggaaca cctgaccaac ggaagttatc gatagtgaat ttgcctgcga cgaatgtttc
    18901 agtctctgat tgagcatcca ctttgtcttc ttcgcgatag cccttgaatt gtacaccatc
    18961 caattcacct tgagcatatt gcccgcgaac gaagttttct aacacttctt cttcgttata
    19021 aatgcggata gctttcaatg ctttgatctt ttctgtacgg atttcttttt cggaaaatgc
    19081 tactggcggt tccattgcaa gtaatgctac aacctgtaaa atatgatttt gcaccatgtc
    19141 tttaagtgcg ccactatgat cgtagtagcc cccgcgatct tctacaccga ggttttcgcc
    19201 aaaagtaatt tggatattgt cgatatagcg attgttccat tgagcctcaa aaatattatt
    19261 ggcaaaacgg atagctgaga tgttttggat catttctttt cctaaataat gatcaatcct
    19321 gaaaatatct tgttctggga atacttctct gatttgctgg ttcaattcaa aagcagactg
    19381 gtaatcagaa ccgaacggtt tttctatgat caaacgattg aagccatttt ttgtcataat
    19441 attttgtgat ttcaaatgag aaacgatcgt tccaaaaaat tgaggtgcca tagccaaata
    19501 atagacacgg ttaccgttta attgatagtg tccatctaat cggtcagaca attcttttaa
    19561 tgtctgataa tgcgttgaat catttacatc atgggattga tagtaaaaat ggcttgcaaa
    19621 ggtagttgct tcttcatctg aaggattcaa tgcacggatt gtttctttga caacttcacg
    19681 gtagtgctca tcgctccagg gacggcgagc ggttccgatt acagcaaaat gctcgcctaa
    19741 atttcctttt ctgtaaaggc ggaacaaaga cgggtacaat ttacgctggg ctaggtcacc
    19801 tgtaccgccg aatatcgtaa acaatacatt tttttcatta gacatgtgga tacactcctt
    19861 tatattttat cgacaaaaat cgtgctggcc gccttaaagc tgacggggat tttctttcta
    19921 tcattttgga tagtgatcgg tccttcataa gctgcgatgt caacgatctc atattcttcg
    19981 ttgatattca ggtcaatagt gactaagtaa tccaacaatt ctttttcatc aagcactcgt
    20041 gcgatacgca cttttgtacc gacaggatag gactccaatg tttgtcggcg ttcttcatgg
    20101 acaacttcct cttttttagg aatcgatcct ccatgaggac aatgttcagg atgattcaaa
    20161 tagttttcta aatgatctgc aagatgttcc gaagtcacat gttccaatac ttcagcatca
    20221 tcatggactt cattccagct atagtctaaa tgttcaacta aaaaaacttc ccataagcga
    20281 tgtttgcgaa tcagactgct agctttttgc agtccgcttg cagtcaattg tacaccttga
    20341 taaggggaat gttctacaag ctcttctttc acaagtttag aaatcatttc actgacagat
    20401 gcagctgaga catctagact agatacgatt tgtttattgt tgactttcgt tgtatctcca
    20461 cctagttcta aaatgatttt caaataatct tctcgatttg gtgtcatatt cgatcatcct
    20521 ttcatggtaa aaaaccatca ttagtaagtg taacaaaaaa aacgttaatt gactattttt
    20581 ctaaataaaa aacaaaggat ttttagacgt ttttacaaca aaaaacgaaa gatgatagag
    20641 aagactcatt ctttcg
//
LOCUS       DAYYME010000041        20377 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000041 DAYYME010000000
VERSION     DAYYME010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.041"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..749
                     /gene="purC"
                     /locus_tag="ACHBFW_001259"
     CDS             21..749
                     /gene="purC"
                     /locus_tag="ACHBFW_001259"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFM9317572.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            749..1003
                     /gene="purS"
                     /locus_tag="ACHBFW_001260"
     CDS             749..1003
                     /gene="purS"
                     /locus_tag="ACHBFW_001260"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFM9317573.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1005..1676
                     /gene="purQ"
                     /locus_tag="ACHBFW_001261"
     CDS             1005..1676
                     /gene="purQ"
                     /locus_tag="ACHBFW_001261"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFM9317574.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1677..3899
                     /gene="purL"
                     /locus_tag="ACHBFW_001262"
     CDS             1677..3899
                     /gene="purL"
                     /locus_tag="ACHBFW_001262"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFM9317575.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            3884..5323
                     /gene="purF"
                     /locus_tag="ACHBFW_001263"
     CDS             3884..5323
                     /gene="purF"
                     /locus_tag="ACHBFW_001263"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFM9317576.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5346..6398
                     /gene="purM"
                     /locus_tag="ACHBFW_001264"
     CDS             5346..6398
                     /gene="purM"
                     /locus_tag="ACHBFW_001264"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFM9317577.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6395..6973
                     /gene="purN"
                     /locus_tag="ACHBFW_001265"
     CDS             6395..6973
                     /gene="purN"
                     /locus_tag="ACHBFW_001265"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFM9317578.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            6970..8505
                     /gene="purH"
                     /locus_tag="ACHBFW_001266"
     CDS             6970..8505
                     /gene="purH"
                     /locus_tag="ACHBFW_001266"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFM9317579.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8521..9795
                     /gene="purD"
                     /locus_tag="ACHBFW_001267"
     CDS             8521..9795
                     /gene="purD"
                     /locus_tag="ACHBFW_001267"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFM9317580.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            9917..10831
                     /locus_tag="ACHBFW_001268"
     CDS             9917..10831
                     /locus_tag="ACHBFW_001268"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFM9317581.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(10964..12529)
                     /locus_tag="ACHBFW_001269"
     CDS             complement(10964..12529)
                     /locus_tag="ACHBFW_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9317582.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12522..13973)
                     /locus_tag="ACHBFW_001270"
     CDS             complement(12522..13973)
                     /locus_tag="ACHBFW_001270"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9317583.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(13987..15003)
                     /locus_tag="ACHBFW_001271"
     CDS             complement(13987..15003)
                     /locus_tag="ACHBFW_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9317584.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15223..16311
                     /gene="chvE"
                     /locus_tag="ACHBFW_001272"
     CDS             15223..16311
                     /gene="chvE"
                     /locus_tag="ACHBFW_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM9317585.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16365..17891
                     /gene="mmsA"
                     /locus_tag="ACHBFW_001273"
     CDS             16365..17891
                     /gene="mmsA"
                     /locus_tag="ACHBFW_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9317586.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            17893..19098
                     /gene="mmsB"
                     /locus_tag="ACHBFW_001274"
     CDS             17893..19098
                     /gene="mmsB"
                     /locus_tag="ACHBFW_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFM9317587.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19355..20332
                     /gene="guaC"
                     /locus_tag="ACHBFW_001275"
     CDS             19355..20332
                     /gene="guaC"
                     /locus_tag="ACHBFW_001275"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFM9317588.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
BASE COUNT         6523 a         3462 c         4674 g         5718 t
ORIGIN      
        1 aataaaaagg agagacgtac atggagaaga aagaactttt gtatgaagga aaagccaaaa
       61 aaatatatct tacttctgat gagaacttga tttggataga atatttagat caagcaactg
      121 cgttgaatgg cgcgaggaaa gaccagataa gtggaaaagg agaattgaat aatcaaatca
      181 caagtgcaat tttccgttat ctgactcaaa gaggaataaa aaatcatttt attgaacaat
      241 tgtctaaaac cgaacaatta gcaaaaaaag tacaaattat ccctctggaa gtggttatcc
      301 gaaatattgc agcaggaagt ttctcaaaac gtctaggtgt tcccgaaggt aaagaattca
      361 tcaaaccaat cattgaattc tactataagg atgaccagtt ggatgatcca tttatcaatg
      421 aagagcatat ctatttctta caacttgcaa agccggaaga aatcagtgag ctgaaagccc
      481 aagcgttggc tattaatgaa attttgaccg aattatttgc taaggtcggt ttggttttag
      541 tagatttcaa gctagaattt ggccggtcga atggacaaat tttgcttgcg gatgaagtaa
      601 caccggatac ttgccgatta tgggatgaac gtacgaatga acatatggac aaagacgttt
      661 accgcagaga tctaggtgat ttgatcccag tctatcaaga agtatataac cgtctatcgg
      721 aactaactga agaaaaggag cgagtatgat gtatttcgta aaagtatatg taacctataa
      781 agattcagtg ttagatcctc aaggggaggc agtaaaaaaa gcagtccatc gtatgggata
      841 tgaaacaatc gaagatatcc gaataggaaa atactttgag atacaacttg ctgatgcaga
      901 agaaccaatc gatacagtga ttgaaacgat ttgtgacaag ctgcttgcaa atgtaaatat
      961 ggaaacatac cgatatgaaa tcaaaaaaat ggaggaagta taacatgaaa tttgcggtca
     1021 tcgtttttcc aggttcaaat tgtgacgcag atatgttatg ggcagttcgc gagatcatgg
     1081 gcgctgatgc cgaatttgta cggcatgatg agtcctcttt agaaggattt gatggggttt
     1141 tactgccggg aggattctcc tatggtgact atttgagatg cggtgcgatt gctcggtttt
     1201 ctacaatcat ggaagaagtg attcgttttg ctgaagaagg gaaaccagta ttcggtacat
     1261 gcaatggatt ccagatattg acagaagcag gacttttgcc aggtgtactg agacgaaatg
     1321 agtctttgca ttttatttgc aaaactgtac cattgaaagt caaccccaag acggccttta
     1381 cttcagagta tgaagccgac gaaataatct atcttccagt tgctcatggg gaaggaaatt
     1441 atttctgtga cgaagaaaca cttcaagaac taaaaaaaaa taatcagatt gcctttactt
     1501 ataatgacaa catcaacggc agcgtggaaa acattgcagg gattacgaac aaagcaggga
     1561 atgtgttagg aatgatgcct catccagaac gtgcagtaga agacttgcta ggatcagaag
     1621 atgggaaacg gttctttgct tccattttaa aaaacttcgg aaaggtgacc gtatagatga
     1681 aaacattgaa agaaccgaca gctgaagaga tcaagaaaaa aagaatctat gccgaatggg
     1741 gattgactga tgaagaatat cgaatgatcg aagaagatat cttaggaaga atgcctaatt
     1801 atacggaaac tggactgttt tctgttatgt ggagcgaaca ttgttcctat aaaaattcta
     1861 aaccggttct acgcaaattt cctactagtg gacctcatgt gttgcaaggc cctggagaag
     1921 gcgcaggcat cgtcgacatc ggtgatggac tagcagttgt cttcaaagca gaaagtcaca
     1981 atcatccttc agccgtggag ccttatgaag gagctgcgac aggagttggc ggaatcatca
     2041 gagatatttt cagcatgggg gctcgtccaa ttgcgttgct agattcattg aggttcggcg
     2101 aattagataa tccaagaacg aaatatttac tagaagaagt agttgcaggt atcggcggat
     2161 acggcaattg tatcggcata ccgactgtag gcggcgaaat tgcatttgat ccttgttatg
     2221 aaggaaaccc attagtcaat gcaatgtgcg taggtttgat tgaacacaaa catatccaga
     2281 aagggcaagc cgctggcgtc ggtaattcga tcatgtatgt aggtgcaaaa acaggtcgcg
     2341 acggtatcca cggagcaact tttgcctcag aagaatttaa tcaagaagaa gaacagcaac
     2401 gttctgctgt acaagtaggc gatcctttta tggaaaagct gctgcttgaa gcgtgtttgg
     2461 aattaatctt gcatcattcg gattctctaa taggtataca ggatatggga gcagcaggac
     2521 ttgtttcatc tagtgcggaa atggcttcaa aagctggctc aggacttata ttgacattag
     2581 atgaagtccc acaaagagaa actgggatga caccttatga aatgatgtta tcggaatcac
     2641 aagaacggat gttgatttgt gttaaatcag gagaggaaga aaaaatccaa gaactatttc
     2701 agaagtatga tctagatgca gtcactatcg gtaaagtaac agacgacggg caatatcggc
     2761 tatatcatca aggagaagaa gtagcaaatc tgccagtcga tgcattagct gaagatgcac
     2821 cagtttatca taaagaaatg aaagaaccag ctcgaatcgt agaatttcag cagttagctc
     2881 cttatcagcc agttatcgaa gaacctggac aagtattgct tgatctgctg cagcaaccga
     2941 cgattgcttc aaaacgttca atctacgaaa cttatgattc acaagtacaa accaatacag
     3001 ttgtccgtcc aggaagcgat gctgctgtga tgagggttag agggacgaat aaagcgttag
     3061 ctatgaccac agactgcaac gcgcgctatc tgtatttgga tccaaaaatc ggaggacaaa
     3121 tagcagtggc agaagctgct agaaatatta ttgctagcgg tggtaagccc ttggcgatca
     3181 ccgactgcct gaactatggt tctcctgata aaccagaagt tttttgggaa ttatcgactt
     3241 cagctgacgg aattgctgct gcgtgtgaaa cgttaggaac tcctgttatt tcaggaaatg
     3301 tctctttata taatgaaacg gatggtcaag caatctatcc aacaccaatg attggtatgg
     3361 tcggattgat tgaggatcat aaacatatca ctactcagga attcaaaaaa tcaggggatc
     3421 taatctatat attaggtaag acgtttgcag attttgatgg cagtgaatta caaaagatgc
     3481 agcttggaag aatcgaaggt gtgatccgaa actttgatct atccattgaa aagagaaacc
     3541 aagagttagt attgacagcc atccaaaacg gattgattga aagtgctcat gactgttcag
     3601 aaggtggctt ggcaattgca ctggcagaat cagctttcaa acatcagcta gggatatctg
     3661 tacagtttga attgtcctct gctcaattat ttgcagaaac tcaatcaaga tttgtgttga
     3721 cagtcgcacc ggaaaataag acacgattcg aagaaatgat gggagatgct gctgttttag
     3781 caggtaaggt gacggatgaa gcgataatcg aaatttcagc aactgacggt cagatcaaaa
     3841 tagaaactgc agtagcaaga aagtgctggg aggatgcaat cgtatgtctt atgaagtgaa
     3901 gagtttaaat gaagaatgcg ggatcttcgg tgtttggggt catccagacg ccgctagtgt
     3961 ggcatacttc gggctccata gtcttcagca tcgaggacaa gaaggtgcag gtatcgtgtc
     4021 caatgatcga ggaaagctta aggggcatcg agatttgggg ctgctttctg aggtattcaa
     4081 agatcaaaga aaattgcagc agttaacagg agaagctgca atcggacatg tacgttatgc
     4141 aacagcaggc aatggaagtg tggacaatat tcagcctttt ctattcaaat ttcatgatca
     4201 gtcgatcggc ttagcgcata atggtaactt gatcaatgct cgttccctta gaaaagagtt
     4261 agaggaaaat ggggcgatct tccattctaa ctccgatacg gagatattga tgcatttgat
     4321 ccgacgaagt gaagaaacag attttttaaa taaattaaaa agtgctttga atgaagtcaa
     4381 aggtggcttt gcctatttat taatgacaga aacggcaatg attgccgctt tggacccaaa
     4441 tggttttcgt ccattagcaa tcggacaaat gaaaaacgga gcatacgtga ttgcctcgga
     4501 aacttgtgca ttagaagtta ttggggcacg ttttataaga gatgtccagc ctggtgagat
     4561 agtgatcatc aatgacgaag gaatccagat tgattgcttt acgaaggaca cgcagctttc
     4621 tatttgttcg atggaatata tctattttgc cagaccagat tcaaacatcg cagggatcaa
     4681 tgtgcatact gcacggaaaa atatgggacg gaatttagcg aaagaatcgc cagtaaaagc
     4741 agatatggtc gttggcgtac ctaattcttc tttgtcagcc gcaagcggtt atgcggaagc
     4801 tagtggaatc ccttatgaaa tcggattagt aaaaaatcaa tacattgccc gaacatttat
     4861 ccagccaacg cctgagctaa gagagcaagg tgttcgaatg aaattatctg ctgtacgagg
     4921 agtagtggaa ggtaaaaagg tgattatggt cgatgattca attgttcggg gtacgacaag
     4981 cagacggatc gtccatcttt tgaaagaagc aggggcaaaa gaagtccatg tgaggattgc
     5041 ttctcctcct ttgaaatatc catgttttta cgggattgat attcaaacgc gtaaagaact
     5101 cattgcggca aatcacacga ttgatgaaat caaagaatgt atcggtgcag attctttagg
     5161 ttttttaagt gaagagggtc tgatcgaatc gatcggtctg gaaaaagatg ctccgtattc
     5221 aggattgtgt atggcttatt tcaacggaga ttatccgacg ccgttatatg actatgaaga
     5281 aaaatatcag gaatcactaa aagaaaaagt atcgttcttt taaaagcaga ccaagaaggg
     5341 agtatatgaa ttcaatggca aatgcatacg caaaagctgg cgtagatgtc gaagctggat
     5401 atgaagtagt agaacggatc aagaagcatg tcaaaaaaac ggaacgttta ggtatgatgg
     5461 gcgctatagg aggattcggc ggttgttttg atttatcggt ctttcaatta aaagagccag
     5521 tccttgtctc ggggaccgat ggcgtgggga cgaaattagc agtggcaatc gaagcagaca
     5581 aacacgatac aatcgggatc gactgcgtag cgatgtgtgt caatgatatc ttagcacaag
     5641 gcgcagagcc gttatatttc ttagactact tagcaacggg gaaaaatgat ccgttacgtt
     5701 tggaaaatgt agtaaaaggt gtagcagatg gatgtgttca aggaaatatg gcgctgattg
     5761 gcggagaaac agctgaaatg cctggtatgt actcaggtaa tgattatgat ctagccggat
     5821 ttgctgtagg gatagctgaa aaatctcaat tattgacagg agaaacgatc aacccaggag
     5881 atgtgttgct tggaatagca tctagcggga ttcattcaaa tgggtactct cttgttcgca
     5941 aaattttctt tgagcagcag cagttttctt tatcatcaaa actgccagaa ttgaaaaaaa
     6001 cacttggcga agaattgctt gagccaacga aaatctatgt ttcttccttg cttccgctga
     6061 tcaaagaaaa gaaaataaaa ggagctgcac atatcactgg tggagggttt gtagaaaata
     6121 tcccgcgaat gttaccagaa tctatggctg cacaaatcaa acttggctca tggccgattc
     6181 ttccaatatt cggctgttta caaaaatatg gtgaaattcc tgagatggag atgtatgaaa
     6241 tcttcaatat gggcatcggt atggtcctgg cagtccaaga atcgaaagta aaagaaatag
     6301 tacacgaaat tgagcaagct ggcgaaaaag tttatgaaat cgggaaagtg atcgccaaag
     6361 aaaaacatcg tgtggtgttt ataggagaag aacgatgaga atcgctgttt ttgcttctgg
     6421 aaacggaagc aactttcaag cgctagccga ttatctatcg aaaaaaggat tggaatcttc
     6481 gattgactgg ttgttttgtg atcagcctga agcatatgtt ttgaaacgtg caactgcgtt
     6541 gagtgtaccg gcagattgtt tttcacctaa agaatttgat tcaaaaaaag agtatgaaga
     6601 agcaattttg cataaattga aagaaaaaaa gatcgatttg atcgtattag ctggatatat
     6661 gcgaatcatt ggaccggtat tgctgaaaaa ttatgataaa agaatcatca atatccatcc
     6721 gtctctttta cctgccttcc ctggtttgca tggcatacgt gatgcctttg aagctggagt
     6781 aaaagaaacg ggtgtcacaa ttcattatat cgaccaaggt gtagatactg gaccaatcat
     6841 ccggcaggaa aaagtgagaa ttgaacagga ggacacgttc gattctttag aagaaaaaat
     6901 ccatagagta gagcatcgaa tctatccaga agtgattagt gaaattattg aaaatggagg
     6961 ctatctgaaa tgacaagagc attgatcagt gtatcagata aaactgggat tgtagcattc
     7021 gcccaaggtt tgattgaaca aggagtagaa atcatttcaa caggaggaac aaagcatgcg
     7081 ttggataaag ctgggatcaa aacgctttcg atcgaagaag tgacagggtt tcctgaaatg
     7141 atggatggcc gtgtcaaaac acttcatcct aaaatccatg gtggtctttt aggaagacgc
     7201 gatctcccta gccatatgga ggcaatggaa acggaaaata tccaaccaat cgactttgtt
     7261 tgtgtcaatt tatatccttt caaagaaacg attatgaaac cagatgtcga aatagcagaa
     7321 gctattgaaa acattgatat cggtggtcct agtatgctcc gtagtgcagc aaaaaaccat
     7381 gaatttgtca cctctgttgt tgatccatct gactatgaag aagtactttc agaattacgt
     7441 cagtatgggc atacttcact ttctttcaga caaaaactag cggcaaaagt ctttcgtcat
     7501 actgcggcat atgatgcact gatcgctcaa tacttaacgg attgggtgga agaaaaagaa
     7561 ccggaaaaat tgacacttac gtatgaacga aaacaaacgc ttcgttacgg tgaaaatagc
     7621 catcaagccg ctgcctttta tcagtccgct cttctggaaa aatattcgat cgcagcagca
     7681 aaacaactcc atgggaaaga actttcttat aataatatcc gagatgcaga tgctgcgcta
     7741 cggatcatga aggaatatga cgagccaacg gtagtcgcct tgaaacacat gaatccttgt
     7801 ggtattggga cagcatctac gattttagaa gcgtgggaag cagcgtatga agcagatcct
     7861 gtttcaatct ttggtgggat catcgttttg aatcgagaag tagatctgcc aacagcagaa
     7921 cagatgcata agctgtttct agaaatcatc attgcccctt cttatacaca agaagctttg
     7981 gaattactgc aaaaaaagaa aaacatccga ttactgacag tagactttgc gcaggaagaa
     8041 aatcaaaatc gagaagaaac ggtctcagtc ttaggtggac ttcttgtaca agatcaagat
     8101 ctggcaatag aaaatgaaga agagtggaaa gtcgtgacaa aacgtaagcc gactccagca
     8161 gaagcaaaag cattagcgtt tgcttggaga gctgtcaaac atgtgaaatc taatgcgatc
     8221 gtactggcaa acgaacgtca gacagtagga atcggagccg ggcagatgaa tcgagtcggt
     8281 tcagtaaaaa ttgcgattga acaagcgcaa gagaaaatgg aaggtgcagt tttagctagc
     8341 gatgcttatt ttccaatgga tgattcggtt gaatatgcag ccaaacatgg tataaaagca
     8401 atcattcagc caggaggaag catcaaagac caagattcta ttgatatggc gaataaatac
     8461 gatctagcaa tggtctttac tgatgttcgg cattttagac attagagagg agtcggataa
     8521 atgaacattt tggtgatcgg tgcaggtggt cgtgaacatg caataagtaa aaaactttta
     8581 gccagtccga aggtggatac ggtgtattgt gcgccaggaa atccggggat gacaaaagat
     8641 ggtatcaatt gtgtcgctat cagtgagcga gatcattcag cgttaatcca ttttgttaag
     8701 aaagaggcta ttgattggag tatcgttggt cctgaagttc ctttgttaaa tgggatcgtc
     8761 gatgatttta tggcggaagg attgaaaatc ttcggtccga caaaagcagc agctctgatc
     8821 gaaggttcga aaagttttgc taaacaatta atggatactt atcaaatccc gacagctaaa
     8881 tccagaagtt ttgaatgtta cgaaaaagca gcagcgtata tcaaagaaaa tggtgcgcca
     8941 atcgtagtga aagcggacgg cttagcagct gggaaaggtg tagtcgtggc taccacagtg
     9001 aaggaagcgc tggatgcggc agaacaaatg ttgaaccagc atcgatttgg ggatagtagc
     9061 aaaaaaatcg tcgtagaaga gtttttggca ggagaagaat tctccctttt ggcgtttgtc
     9121 cgtaatacca aggtgtatcc gatggtcatt tcgcaagatc ataagcgggc atttgataac
     9181 gataaagggc caaatacagg cggaatgggt gcttattcac cagtaccgca aatttcaaaa
     9241 aagatgattg aaaaagctgt ggaagaagtt ttaaaaccag cggctttggg aatgaaagaa
     9301 ctaggacgct ctttcacggg tgttttatat gcgggtttga ttgctacgaa ggaaggacca
     9361 aaagtgatcg aattcaatgc gcgtttcggt gatcctgaaa cgcaagtcgt tctttctcgg
     9421 ttgaagagtg actttgcgca agtaatcgat gatttattag agaatcgtac agtagagtta
     9481 aagtggcagc aagaaggata caatgtaggc gttgtggttg cagctgccgg ttaccccgaa
     9541 gaatatgaga caggaatggt tctaccagat tttctggaag aagagctttc tgtttactat
     9601 gctggagttt cctcaaataa cggcaaacta gttggagcag gaggacgtct ttatttagta
     9661 gaagcatacg gtgagacgat caaagaagca cagaaaaaaa tttacaccgc gttaatggat
     9721 aaaacgacag atggaacatt ttatcgtaca gatattggag aaaaagctgc caatagcgaa
     9781 ctgatcgtga agtaaatcag ctgcttccat ttataaaaaa tacaacaaac ataatcggaa
     9841 gcgccgattt tagtgaagcc ttttcataaa gagtgtggta cacttttgtt gtgtatataa
     9901 ataaggagga gttcagatga aaaaaattca attaggtact tctgatcaga aagcagctgc
     9961 agtgatctta ggctgtatgc gtatcaacaa tgcggaagat ccagtaaaag tgattgagac
    10021 agcttatgaa aacggcattg atttttttga ccatgcagat atttatggaa acggtgaatg
    10081 tgaagaaatc tttgcagatg ctctagcaaa aacatcaatc aaacgagaag atctttttat
    10141 tcaaacaaaa tgtggaatcg ttccaggtgt gatgtttgat ttttctaaaa aacatatcat
    10201 tgaatctgta gaaaaaagtt tgacccgtct gaaaatggat tatgtggatg ctttacttct
    10261 tcatcgtcca gatacattgg tagaaccaga ggaagtagct gaagcgtttg atgaattgga
    10321 acggtcagga aaagttcgtt attttggcgt cagcaatcaa aaaccaatgc aaatcgaatt
    10381 gctgaaaaaa tatgttcgtc aaccattagt agctaatcag ctgcaatttg gcttgaaaca
    10441 tactggaatg atcgatcaag ggattcatgt caatatgaca gatgaagcaa gtatcgatca
    10501 tgacggtagt gtacttgatt attcccgatt gcatgatatg acgatccaag catggtcacc
    10561 ataccagtat ggattttttg aaggtgtatt tatcgggaac gaaaaattcc ctgaattaaa
    10621 ccaaacatta gaagaactag cagctaaata tcaaacgaca ccaactggct tggcttctgc
    10681 ttggatttta cgccatccag ccaatatgca agtgatcgcc ggtacgatga acgtagggcg
    10741 aatcaaggaa atcgctcaag cttcagaaat taatatgtct cgggaagatt ggtacgaatt
    10801 gtaccgtgct gctggaaaca ttttacctta aagattgtca taagcaggtc aaaagggcta
    10861 taaattaaaa agacagaata acttgaaagc tgttttcagc ttcgagatat tctgtctttt
    10921 tttattgcaa atggaaagag tatcattgta cggatttcgt ttattagtta tttttgtttt
    10981 taaggaaaag ctgtgtccga tattcttttg gagaaatccc ttgttttttc ttaaataaat
    11041 aactgaaata gttagggtca ttataaccaa tttcaaaagc aatatctgaa atacgatagt
    11101 ctgattgctg caataatttt tttgctaatt cgattctttg ttctgtcaga taatcgatga
    11161 atgtttggtt aaacgattga gaaaaaatcg tactgaaatg agagggactt aatgcaactt
    11221 gttgagcgac ccaagttaag gacatatctg gatcggtaaa attttctttg ataaaatcca
    11281 atgcatggtt caaaattggc tgatatttgt tccttgaagg ctgtgagggc agagcagtta
    11341 agaaagaaat gaacatataa actgtcgaac ggtaaagcga gtcgtcggcg ctgattgcta
    11401 agcgttgact ggtggatgtg caatgaacca tttcttgcgg gattttcttt tctttcaggc
    11461 agacatgctg gagtagctta gtcaagacaa ataaacgata taatcgtgtt cgagttggtg
    11521 tgccttgtgg tttcgtcaag tgttcgatgt acttagatac ttcgtttttc gataatgaag
    11581 acaaatcaag tgagaggtca aagtaaaaga cagaatcaac tggaagtttc gcttcttgaa
    11641 tcaaatcctc atagctgata atttgttctt taagggaact ggcggtttca agtatcatct
    11701 gcgtgatctc aaaagatgca ggtaattcgc tgattctttc tgcgacagga ccaatggcga
    11761 cagaaaaatt atttttttgt cccttttcta attcatggac caacgtttgg gctacctgat
    11821 aactggtctc gatcacattt tcttcaagag aatcaactat taaaaatttg atgaatcgag
    11881 aggaaacagc agagaagatc acttgttgat tctccctaaa taaaaacttc aaataatcac
    11941 tgagatgagc gtaatcttca aagtctgtac tgacttggtt tgctgctaat aatacggtca
    12001 tttttttccc tgctaaagag cgattgaaag attcagcaag tttcaatgtt tcactgatcg
    12061 taagtgagcc tttgaacaaa ccgttcaaga aatgattttt tttcaattcg aaaacaagat
    12121 cagaagaagt gtgcgtttct ttttgctgca aatctaaagc agttactgct ttttctattg
    12181 cttgtttcaa ttcgctactt ttaatcggtt taagcagata atcatctgcc tgtacctgga
    12241 ttgctccttt catataatcg aaatcgtcga atccactaat aaatatgaga cgagtccaag
    12301 gaagtagttt ccttgcttct tttgaaaaag tcaacccgtc catgaaaggc atttggatat
    12361 cagtcagaat gatgtcgggc tgtaagtcga taattgaaga cagtgccatc tcaccatctc
    12421 cagcttcgcc gacataagtg attggataat aagaaccaag gacagttatt tgttcccgca
    12481 agttattgcg aatgaggtat tcgtcttcga tgataaaaac tttatacatg tttgtttcct
    12541 ccaattgttt tgggagatag agggatcatc agtgtgacag ttgttccttc tcggtatttg
    12601 ctgtcaatcg tgataggttt ttgatcattt ccgtaataaa gaaggagacg cttgtttacg
    12661 ttgtaaagtc cgtagccgat actgaaatct gaatcgatcc ctttggctaa ctctttacgt
    12721 atttctgcca gtttttctgc agggatgcca atcccgttgt ctataacttt gaattggatc
    12781 atctcatcaa ttttgcatgt ggtaatacga ataaatcctt tgtgacggac gtacttcgtt
    12841 ccatggtata cagcattttc aatcaatggc tgcaagatca tttttaaaat agaaacagga
    12901 aaaagtgttt ctggtatatc gatcttatag tcaagcatat ggccgtatct tatttttaag
    12961 atgactagat aatcattgat atggctaatt tctttttcaa gaggaatcca gtcttgtcct
    13021 ttgcttaaag acgtacgaaa aaagccggaa agtgcatggg tcatagtcag agcttcttca
    13081 aagcagtctt gttcgatcaa agaaataatg gcatccaaac tgttataaat aaaatgggga
    13141 gtaatctgag cttgcaatac acgaacttca ctttgtgcta aatggtattg tttcaaagcg
    13201 ttctcccgta aagactggtt tagatcttcc gccattttat tcatgctagc ggtaagtgtt
    13261 tgtaattcca gtgtttccgg cagggttaaa cgaaagccaa ggttccctga agaaagttgt
    13321 tctgacatct cgatcagctg attgatcggt tcttggacct ttctctctaa aaagcgacga
    13381 ttctttttcg catagaaaaa aatggtacta agaactccaa gttccactat tgttaataga
    13441 atgagcgagc ggacaagtgt cgtatttttt ttgcttgcta agtcgatctc cacacgaaca
    13501 aaatcttgta agatatctga cagcagttga gtgacagaat ctacctgatt gatgatctct
    13561 tcattggtat ccataaaatc ttttgccaag atattattga tcagctcgtt ttgataatct
    13621 tccagtgtat cgatcgtacg tagcgataca tctaacgtac taatttcttt aagtgtattt
    13681 gtattatttt tgatctctcg aatatcattc ctaagttctt ctaggatatt ttcctcattg
    13741 aactcatcta cggttacttg gccagaaatc aagtcccaca tactttccaa aactttttga
    13801 tcaaccttca aagcaattgc atctgctttt tggatatttg cgatcgtctg ttgataaatc
    13861 aaaagctgtt gcgaatataa cagtatgctc agtagcaaag ggataacagc aaatagaagc
    13921 atggctttgt ttgcttgggc aaaaatctgt tttatgctta aattttttgt catgatactt
    13981 ctccagtcaa taatttctgg ttgggatgtt agctatattc tgctgggaaa agacagtctc
    14041 ggaaataaaa atatctttag gtaatgtttt tcctgtttga atatttccaa aataacgagt
    14101 aacggtattg gctacatacc agccgacatc aggtttacac tcgactacac aattgacgat
    14161 tcctgagcgt agcttctcga tcatatcttc ttgtccatca atagttacaa tgatcaaatc
    14221 ttctccaggt ttgattgatg tttcttttat cgcagctaaa gcaccgtgag tcatttcatc
    14281 actatggctg tataatacat cgatttcgga taaatcattt gtttctaaat atcgtttcat
    14341 ctggtctttt cctttcatcc gaataaaatc accgacaatt cggtgaacaa tagtcatcgg
    14401 aggtttatgg tcgatggttc catctttttt tagggttggt ttacgattga ttgattccaa
    14461 aaatccttcg gagcgtagct tggtcggtgt ggagttctct gaaccagcaa gttctaggat
    14521 tctgatttcc tgtttctttg aatttgtgaa gtgattgctg acaaataaac ccgcccgatt
    14581 tccttctgct ttgaaactgg ggccaatgtg ggtgacgaaa agctcgggat cattcgtttg
    14641 aatatgtctg tcgacaagta tgacagggat ccctgctcgc tttgcttctt ttaatacaga
    14701 gtcccacccg gattcttgca aaggagtaaa aatgatcata tccactttat aggcaatgaa
    14761 cgtccgtata tcttggattt gccgttcctg attcatgtag ccatttcggt agagaacttg
    14821 gtaatcttct ttttcaagcg cagtgataat tgattcagta tgctttttcc gccagctact
    14881 ctccgttcct gattgcgaaa aaccaacgac tagtgggtca ggtgcttcct ttgtattagg
    14941 tgtacacccc atcatttggt aagcgattac aacaagggct aaaaaaaata aaacacgttt
    15001 cactttaaat cactccatct ggatttgtta gtactatctt atcagataaa agggaaaatc
    15061 aaagtcaaaa ggaatcaatc gaaaaaaatc caagaaactt aaaaaaatcc aagacaaaaa
    15121 aagtacacct aaaaaattta aagtaaatcc cataaaaaaa tcaatgttaa cgctttcttt
    15181 attagctata ctcggtgtat caattgaaat gggggataaa aaatgaaaaa caaatttaga
    15241 aagattgttg ggactatcgc tattttgacc gcaagcattc tattaagtgc gtgtggcaat
    15301 tcaggatctg cagatgatag tacggggtat gtaggaatcg ctatgccgac caaatcagca
    15361 gaaagatgga tcgctgatgg gaacaacatg gtctcggaat tagaaaaact gggttataaa
    15421 acagatcttc aatatggaga agataaagta gaaaatcaag tagcacaaat agaaaatatg
    15481 attacaaaag gggtagatac acttgtgatt gcttctatcg atggttcggc tttaactgat
    15541 gtgttagcta aagcaaaaga agcagatatc aaggtgatcg cctatgatcg tttactgatg
    15601 aattcagaaa atgtcgatta ttacgcgact tttgataatt ttggagtagg tgtatcgcaa
    15661 gcagcttata ttgaagagca cttaggattg aaagaaggaa aaggaccatt cacgattgaa
    15721 ttatttggtg gctcgcctga cgacaacaac gcactaatca attacaacgg agtgatgtct
    15781 gttttacaac catatatgga caacggtcag ttggtcgtgc catctggaca gacgagtttc
    15841 agtcaaatcg ctacgcttcg ctgggatggt tctacggcgc aagcaaggat ggataatctg
    15901 ttaagcgcaa attatactga ccaaacattg gatgctgttt tgtcgccata tgatccaata
    15961 agtttaggaa tcatttcgtc attaaaaggc gtggggtatg ggagtgaaag taaaccttta
    16021 cctgtgatta ctggacaaga tgcgacagtt gctggcgtta aatcgatcat tgccggggaa
    16081 caaacacaaa cgatctttaa agacacacgt atcttagcaa aaaatacgat tgaaatgatc
    16141 aaagcaatca gtgatggaga agaggttccg gtcaatgata ctgaaactta tgataatggg
    16201 gtgaaaactg tccctacgta tttagcaaat acagtatctg tcgataaaga taattatcaa
    16261 gcagagctga tcgacaccga ttattataaa gaatccgatc tgaaaaatta agaggaacca
    16321 aacaaggaac taaagagatc taagtcagag agaggataag aattatggcg gattgtattt
    16381 taaaaatggc agatatcacc aaaaaatttt ctggaatccg agcgttagat tgcgtgaatc
    16441 tgatgattga acgtggggaa atccatgcat tgtgcggaga aaacggagcg ggaaaatcaa
    16501 cattgatgaa tgtcttgagc ggcttgtatc catatggctc ctatgacggg gaaatctttt
    16561 atgatggaaa gctgtgtcaa tttaaagatt tgaaagatag tgaagaaaaa ggcatcgtca
    16621 tcattcatca agaattagca ctaagtcctt atctttcaat aaaagagaat atttttctcg
    16681 gaaatgaaca agcagaaaga ggcgtgattg attgggaact gacagaaaag aaaacatatg
    16741 aactgctgaa taaagtcggt ttgtctatcg acccgaatct tctagtatcc cagattggcg
    16801 tagggcaaca gcaactagtt gaaatagcta aagcattatc aaaatccgtc cgtctgttga
    16861 ttttggatga gcctaccgct gcattgaatg aagaagaaag cgcgaatttg ctgagattga
    16921 tccgagagtt gaaagagcaa ggtgtaacct ccatcattat ttcacataag ctgaatgaaa
    16981 tcgtggatat cgcagatcga atcaccattt tacgggatgg acagacgatt gagacactag
    17041 aaaatgaaat gatcagcgaa gagcatatta ttcgcggaat ggtgggcaga gatttagttg
    17101 atcgttatcc tgaaagaaaa ccggatattg gagaagttta ttttaaagta gaagactgga
    17161 cggtctatca ccctcttgat cacgagcgaa tcataaacga ccacatcaac ttgcatttga
    17221 aaaaaggaga gattgtagga atagctggac tgatgggagc gggcagaact gaattcgcta
    17281 tgagtctatt tggacattct tatggaagca agatatccgg acgtatatta aaggaagggg
    17341 cagagatatc agttaagacc gttccttcag ccattgcaaa cggactcgct tatgtctcgg
    17401 aggaccgaaa atctttagga ctgaatctgc tgatggatat tcgggaaaac gtgacgctga
    17461 gcagtttgga taaaataagt caaaaaggag tattagataa agaaaaagaa gtaactgagg
    17521 cagaaaattt ccggaaaaag atgcgtataa aaacaaactc catccatcaa attgtcagta
    17581 gtcttagtgg tgggaatcaa caaaaagtcg ttttatccaa gtggctgatg acacaacccg
    17641 atattctttt tttggatgag ccaacccgtg ggatagatgt tggggcaaaa tatgagatct
    17701 acacgattat cgaagaaatg gcagcaaatg gcaaagctgt ctgtcttatc tcttctgaac
    17761 ttcctgaaat attagggatg tgtgatcgaa tctatacgat gaacgaagga cgtattacag
    17821 gagaagtctc gcgagaagat gcagatcagg aagtcttgat gaaattaatg acgaaagaag
    17881 aggtgggtta agttggaaca ggcaaaaaat cttacggata aaaaagagaa aaaactaaat
    17941 ttgaaaagta gagcagctga tctttttggt cgctacagca tggtgatcat tttagcggga
    18001 ctacttattg cgtttcaatt gatgacggat ggtattttct ggcgtccatt gaatataact
    18061 aaccttgttc tgcaaaatag ccatatatta gtactagctg cagggatgtt attagttgtg
    18121 ctcttaggtc atgtggattt gtcggtaggg tcagtgatgg cgtttgttgg tgcaattgcg
    18181 ggcatgatga tggtcaatcg ccaaatgaac ccgtggatga cagttatcgc atgtcttgca
    18241 gtaggcgggc tgatcggcgc atggcaaggt ttttgggtag cttatgcagg gataccggcg
    18301 tttatcgtga ctttagcagg attgctgatg tttcgtggac tgacacaagt tgttctagga
    18361 ggccaatctc tagcaccttt tccagctgtt ttccaaaaaa tgtctacggg ttatttacca
    18421 gacttaagcg aaggaagcag ccttcacttc ttgacattga ttttaggaat gatcatcacg
    18481 atcgtgttag ttgttggtca atggcgaaca agaaatcgtc ggaaaaatca tttatttgaa
    18541 gtggaatctc caacagcttt cttggtaaag gcggtattta cagcagtcgt tgtactagga
    18601 ataacttata tctttgcctc ttatcaaggg tatccgatta ttttgatcat tcttggtttg
    18661 atcgttgcag catatgggtt tttgacaaat aaaacagtag caggtcgaca gatttatgcg
    18721 acgggcggaa atcgaaaagc ggctgaacta tcagggatca aaacaaagcg aatcacgttc
    18781 tgggtattcg tcaacatggg agtcatggca gctttagctg gattagtttt agctgctcga
    18841 ttgaatgcgg caacacctca agcaggtaca tcgatggaac tggatgcaat ggcggcagta
    18901 tattttggtg gtgcttcgac ttccggcggc attggaacaa tcatgggtgc gattgtcggt
    18961 ggcttggtca tgggtgtatt gaacaatggc atgtcgattt taggcgtcgg ggtagactgg
    19021 cagcaggcaa tcaaaggatt gatcttgctt ttagccgttg tgctagactt gtataataag
    19081 aaaaaacgaa caacgtagga gaacgccggt tattcagaaa aaagactgtg acaagacatt
    19141 tgtcacagtc tttttttcgc taaaagcgaa cattcattgt ttcgtttgta tagactaaaa
    19201 ataatatatg tttttttacc attcgtataa attctctttt tgctcttgat ttttttgctt
    19261 aattgagtta aattacatag ggcgctaaaa aaagccttga ttaaattaat aatgttcgtg
    19321 ttttgctgga tgtgaacatc aggaggataa tgtaatgaaa gtatttgatt acgaagatat
    19381 tcaattaatt cctaataaat gtatcgtaaa tagccgttct gaatgtgata caacggtcac
    19441 attaggtaaa catacattca aaatgccagt agtgcctgcc aacatgcaga cgattattga
    19501 tgaaacaatc gcagaattct tagcggaaaa tggttatttc tatatcatgc atcggtttga
    19561 tgaagcagct cgaattccgt ttatcaaaaa aatgaaaaag cgtggattga ttacctcgat
    19621 cagtgttggc gtcaagaaag aggaatattc ctttatagag aagttggcag aagaatcgct
    19681 aaatcctgat tatatcacta tcgacatcgc gcatggacat gctaactcag ttattgatat
    19741 gattcagcat attaaaaaat atttgcctga aacttttgtg attgccggta atgttggtac
    19801 accggaagcc gtccgtgaat tggaaaatgc gggcgcagat gcaacaaaag tcgggattgg
    19861 tccaggaaaa gtatgtatta caaaaatcaa gacaggcttt gggactggag gctggcaact
    19921 ggcagctctt agatggtgtg cgaaagcagc tcgcaagccg atcatcgctg acggtgggat
    19981 cagaacacat ggcgatattg caaaatctgt ccgttttggt gcgacaatgg tcatgattgg
    20041 ttcattgttt gccggtcacg aggaatctcc tggcgaaaca aaagtcgaaa atggaatcgt
    20101 gtataaagaa tactttggtt ctgcttcaga attccaaaaa ggcgaaaaac gaaatgttga
    20161 aggaaagaaa atctggatcc aacacaaagg atctttgaaa gatacactag tggaaatgca
    20221 acaagacctt caatcatcta tttcttatgc tggaggaaga gatctggaag ccattcgaaa
    20281 agttgactat gtcatcgtga aaaattcgat ttttaatgga gacacaatct aaaaaataaa
    20341 taaaatatta ggatgttgtg aaaaagttgt cctgcat
//
LOCUS       DAYYME010000042        20296 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000042 DAYYME010000000
VERSION     DAYYME010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.042"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..3374
                     /locus_tag="ACHBFW_001276"
     CDS             <1..3374
                     /locus_tag="ACHBFW_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9317589.1"
                     /translation="KLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAEVISDQS
                     GKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITLEISSNT
                     KQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIEEESSKE
                     NENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVLDHQGNK
                     NGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDVYLNARG
                     NVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINMGYVGYS
                     SEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPNGHKKVI
                     VLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQNNLSRR
                     IDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSSDEKGDL
                     YYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVGTSPTTV
                     TPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTTFQPNID
                     TNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAAAWKNGY
                     IRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIKELPVPG
                     YDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLTDHGDGT
                     YSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVTTSDDTI
                     QLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQTTNEKGL
                     ASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNVWTLTHQ
                     NRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLKPGTYTL
                     TETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITNQAKVPL
                     PETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            3377..4798
                     /locus_tag="ACHBFW_001277"
     CDS             3377..4798
                     /locus_tag="ACHBFW_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM9317590.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            4795..6672
                     /locus_tag="ACHBFW_001278"
     CDS             4795..6672
                     /locus_tag="ACHBFW_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM9317591.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            6764..7606
                     /locus_tag="ACHBFW_001279"
     CDS             6764..7606
                     /locus_tag="ACHBFW_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9317592.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(7758..8633)
                     /locus_tag="ACHBFW_001280"
     CDS             complement(7758..8633)
                     /locus_tag="ACHBFW_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9317593.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            8840..9871
                     /locus_tag="ACHBFW_001281"
     CDS             8840..9871
                     /locus_tag="ACHBFW_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9317594.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            9960..10265
                     /locus_tag="ACHBFW_001282"
     CDS             9960..10265
                     /locus_tag="ACHBFW_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFM9317595.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            10444..12459
                     /locus_tag="ACHBFW_001283"
     CDS             10444..12459
                     /locus_tag="ACHBFW_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9317596.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            12461..12745
                     /locus_tag="ACHBFW_001284"
     CDS             12461..12745
                     /locus_tag="ACHBFW_001284"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9317597.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            12771..14144
                     /locus_tag="ACHBFW_001285"
     CDS             12771..14144
                     /locus_tag="ACHBFW_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM9317598.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(14227..16242)
                     /locus_tag="ACHBFW_001286"
     CDS             complement(14227..16242)
                     /locus_tag="ACHBFW_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFM9317599.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16264..17478)
                     /locus_tag="ACHBFW_001287"
     CDS             complement(16264..17478)
                     /locus_tag="ACHBFW_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9317600.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            17699..18643
                     /locus_tag="ACHBFW_001288"
     CDS             17699..18643
                     /locus_tag="ACHBFW_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM9317601.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLKIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            18939..19883
                     /locus_tag="ACHBFW_001289"
     CDS             18939..19883
                     /locus_tag="ACHBFW_001289"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM9317602.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         6929 a         3718 c         3866 g         5783 t
ORIGIN      
        1 agaaactgaa agttattttc atgctgataa tattgagttt atcaaacttt gtgccattat
       61 ctgcaatagc agacactaca gatgatccaa cagttttaga aacaatttca gctgaagtca
      121 tttcggatca gtctggaaaa aaagcactga acatcaagct aaatgcgaat aacaccagtg
      181 ctgaaaagat agaaaaagaa attggtctag tcgaaaatta cttaagtgat gtggaaagaa
      241 aagaaggaga tggctatgct tatcaggtaa atagcgggaa aattacgttg gaaatctcat
      301 caaacactaa acaaactatc gatctgagtt ttccaatcga tccagcactt taccacagcc
      361 aggcaaacaa gctgatcgtc gataataaag aatatgacat tattgatgag acagaaaata
      421 agaaagatac agatgtgtca gtaccaaagc cagacgaaat agaagaagaa tcatcaaaag
      481 aaaacgaaaa ttctgtcagc ccatttacat tgcctacatt atccttgcca gctgtgagtg
      541 tgccatctaa tcaaacgatt cctacagaat atacaacaga tgatcagggc acttatccta
      601 aagccagttg gcaacctaca ggaaatacaa atgttcttga tcatcaaggc aataaaaacg
      661 gaacaaatca atgggatggt ataaattctt ggaatggaga tcctaatgat cggacccatt
      721 cgtatatcga atatggagga accggtaatc aagcagacta tgcgatacga aagtatgcaa
      781 aggaaacaag tacacccgga ttgtttgatg tttatttgaa tgctcgtgga aatgtacaaa
      841 aagatatcac gcctcttgat ctcgtattgg tcgtagactg gtcaggaagt atgaacgaca
      901 ataatcggat cggtgaagta aagattggtg tcgatcgttt tgtcgatact ttagcagata
      961 gcggtatcac agacaaaatc aatatgggat atgtcggcta ctcaagcgaa ggatatagct
     1021 acagtaacgg tgcagtacag atgggttcat ttgattcagt gaaaaatcaa gtaaaatcca
     1081 ttacaccttc acggacaaat ggtggtactt ttacacaaaa agcactaaga gatgcaggaa
     1141 gcatgctatc cgttccaaat ggacataaaa aagtgatcgt tttgctgacg gatggtgtac
     1201 caacattttc ctataaagta cagcgggtac acgcacaatc aagcagcaat tattacggaa
     1261 ctcagttttc taatacgcaa gatcggccgg gaaatacttc tctaatctca agaatctatg
     1321 atgcacctga ccaaaacaat ctatccagaa gaatcgacag tacgtttatc gcaaccatcg
     1381 gagaagcgat ggcactcaaa gaacgaggaa tcgaaataca tggtcttggc atccaacttc
     1441 aaagcgatcc ggcagctggt ctctcaaaag cagaagtaga gtctcgtatg cgacaaatgg
     1501 tttcatcaga tgaaaaaggc gatctttact atgaatcagc tgatcatgca acagatatct
     1561 ctgaatacct agccaaaaaa gctgtacaga tctcagcaac tgtaagcaat ggacaaataa
     1621 atgatccaat cgcagaacca ttcatttatc agcctggtac actttcagtc aagagtgtgg
     1681 ggacaagtcc tacaacggtc actccatcta tttccataga aggaaatacc atcaagagca
     1741 atcagatcta tttaggaaaa gaccaagaaa tccaaatcca ttaccaagtg agaatccaaa
     1801 cagaaaatga ggacttccat ccaaatttct ggtatcaaat gaacggcagg acaactttcc
     1861 agccaaacat tgataccaat gaattagctg aattcggtat accatctgct aaagctcccg
     1921 gagtcagtct tcacatcaaa aagttatggg aagaatttga caacaatcta gctgatcgtc
     1981 cagatcaagt tacttttgag attcaacggg aacatacgac aaatgctgca gcttggaaaa
     2041 acggatatat tcgaatcatt aaaccagcta aagatacaac aaatacgtgg gaacgtgcag
     2101 acattgacaa attatctgca aatagcggag aaagttatca agagatatta tcactacctc
     2161 aatacaataa tcaaggtcaa gcattcagtt accaaacaat caaagaatta cctgtaccag
     2221 gatacgattc tcaacaaata gatgcaatga catggaaaaa tactaaacaa ttcacaccgt
     2281 taaacttgaa aataacgaaa aattcctcta caggtgaaaa ggatcttatt ggcgctgttt
     2341 tcaaattaac aggagattct attgatactt tactaacaga tcatggcgac ggaacctatt
     2401 ctcttccaga aaatgtcaaa ttgcaaaaag aaatgaccta tacgctgaca gaaacaaaag
     2461 ctccagaagg gcatggatta agcaaaaaga ctacttggga aatcaagatc gcttctgatg
     2521 gtacggtaac cattgatgga aaaacagtca ctacttccga tgatacgatc cagttgacta
     2581 ttgaaaatcc ttttgttgaa gttcctgtag cagtacgtaa gtatgcgatg caagggacgg
     2641 acaaagagat aaatcttaaa ggagcagcat tttccctaca gaaaaaagaa gcaaatggta
     2701 cttatcagcc aattgacagc caaacaacga atgaaaaagg tcttgccagt tttgattcac
     2761 tcacacctgg taaatatcga gtcgttgaaa cagctggtcc tgccggatat gatacttcgc
     2821 cgggaaatta tgaattccaa atcgataaat atggaaaaat catttacacg ggaaaaaata
     2881 ccgagatgac aaataatgta tggacgctca ctcatcaaaa tcgactaaaa gcgtttgatc
     2941 taacggtaca caaaaaagaa gacaacggac agacattaaa aggagcaaaa ttcagactgc
     3001 agggaccaga aatggactta gaatcgccaa aagatggaca agaaacagat acctttctat
     3061 tcgaaaattt aaaacctgga acttatacgc tgaccgaaac ttttacacca gaaggatacc
     3121 aaggtctaaa agagccagtt actatagtta tacacgaaga tgggtcaatt caagtggatg
     3181 gacaagatca tgaatctgtt ctgtcaccag gagccaaaaa caaccagatt tctttagaca
     3241 tcacgaatca ggcaaaagta ccattacctg aaacgggagg aattggccgt ttaggaatct
     3301 atctagtagg gatgattggt tgtgcgtttt ctatttggta tctttttttg aaaaaagaaa
     3361 gagggggcag ctaaatatga aaaaacttgg ttggcttagt atgtgtctct tcttgttact
     3421 atttaaacca gcttttactc aggtagcaac agaaacagaa acagaaatgg ttcagattac
     3481 tttacacaaa ttgcttttcc caaacgggca actgccgaaa aatcatccaa atgacggaca
     3541 agaaaaagct ttattacaaa cgtatcgagg attaaatggt gtcacattcc aagtttatga
     3601 tgtcacagat tctttttacc atctacggga aaagggcaaa acggtagaag aagcacaagc
     3661 agagatcgca aaaaacggtg cgtcttccgg tatgtttacc gcagaagcaa caactacaac
     3721 tcttaacaac gaagatggta tcgcttcttt ttctctggcc gctaaagatc aagaaaaaag
     3781 agataaagcg tatcttttca ttgaatccaa agtaccagaa gtcgtcaaag aaaaggcaga
     3841 gaatatggta gttgttcttc ctgtacatgg acaaaacaat caaaaacttt caactatcca
     3901 tttgtatcct aaaaatgaag aaaacgacta ccctgatcca ccttttgaga aggtattaga
     3961 agagcctaga aatgatttta cgattggtga aaaaatcact tattccttgc atacgacaat
     4021 tcctgtaaat atccttgact atcaaaagtt cgaattgtca gatagtgcgg atgaagcatt
     4081 aacgttttta cctaatagtt taacgatttc atcgaatgga gaaaagctga cagaaggctt
     4141 tgtcatacac aagaaacctc acggatttga tgttttattt tcgatccctt cgttggaaaa
     4201 atatgctgga aaaaaactga ccatttctta tcagatgcag ctaagcagta cagcacaggc
     4261 gaacaaggaa atcaacaaca acggaacact ggattttggt tttggtgtca gtacaaagaa
     4321 agtctctgta tatacaggga gtaagcaatt tgtcaaaatc gagacaaata aaccagataa
     4381 acgattagct ggcgcagtat tccttattaa aaacaaagca ggaaattacc tccagcaaac
     4441 agccaacgga tacaagtgga caaagaacga atcagatgcg cttcacctga tttccgataa
     4501 aaatggcgct ttttcaattt ccgggttgaa aacaggaagt tatcgattaa aagagatcga
     4561 agcaccttct ggttatattt taagtgaaac agaaattccg tttaccattt caacttttct
     4621 ttctgaggat aaagaggcgg acagtatatt gaaagtagtc aataaaaaag aaaatagccg
     4681 tccatttctt ccaaaaacaa acgaaacgaa aaatacactt ttaggcgttg ttggtatggt
     4741 attcgcaagc tttgcaatct ggttgtttat caaaaaaaga acaggagtga aaaaatgaaa
     4801 aatcataaaa aaataaacgt tatgttagga gtccttttcc ttattttacc attactcaca
     4861 aacagcttcg gcgcaaaaaa agtgtttgca gaggagacag cagctcaagt catccttcat
     4921 aaaaagaaaa tgactgattt acccgatcct ttaatccaaa acagcgggaa agaaatgagc
     4981 gaattcgatc aataccaagg attagccgat atttcatttt cagtttataa cgtcactcaa
     5041 gaattttatg cgcaacgaga taaaggagcg tccgtggatg cagcaaaaca agcagtccag
     5101 tctttgactc ctggtacacc agttgcttca ggaacgacag atgctgatgg aaatgtcact
     5161 ttatctttac ctaaaaaaca aaatgggaaa gatgcagtct acacgatcaa agaagaacca
     5221 aaagacggag tgtcagctgc cgcaaacatg gttttagctt tccctgtata tgagatgatc
     5281 aaacaagcag atggctctta taaatacggg acagaagaac tagatactat ccatctctac
     5341 cctaaaaata cagtcggtaa tgatggaacg ttgaaagtta caaaaatcgg tactgccgaa
     5401 aacgaagcac taaatggagc agaatttatt atttctaaag aagaaggaac accaagcgtc
     5461 aaaaaataca tccaaagtgt cacagatgga ttgtacactt ggacaactga tcaaaccaaa
     5521 gccaaacatt tcattactgg tcattcttat gacatcggca acaatgactt tgccgaggca
     5581 tctattgaaa aaggccagtt gatcgttaat catttagaag ttggaaaata taatttagaa
     5641 gaagtaaaag ctcctgataa tgcggaaatg attgaaaagc aaacaatcac gccttttgag
     5701 atcctggcaa atagccaaac accagtagaa aagaccatca aaaatgatac gtctaaagtt
     5761 gataaaacaa cacctcaatt gaatggaaaa gatgtcgcaa tcggtgaaaa aattcaatat
     5821 gagatttctg tcaatatccc attaggtatc gctgataaag aaggaacgca aaacaagtac
     5881 acaacattca aacttatcga tactcatgac gctgctttaa catttgataa tgattcttca
     5941 ggaacgtatg cttatgcctt atatgatgga aataaagaaa tcgacccagt aaattattct
     6001 gtcactgagc aaacagacgg attcacggtt tcagttgatc cgaattatat tccttcatta
     6061 actcctggcg gtacattgaa attcgtttac tatatgcatt tgaacgaaaa agcagatcca
     6121 accaaaggat tttctaacca agcaaatgtc gataacgggc atacaaatga tcaaacacca
     6181 ccgtcagtcg atgtcgttac tgggggcaaa cgatttgtta aagtagatgg tgacgttaca
     6241 tcagaccaaa cacttgctgg agcagaattc gtcgttcgtg atcaagatag tgacacagcg
     6301 aaatatttat cgatcgaccc atccacaaaa gccgtcagct gggtatcggc gaaagaatca
     6361 gcaacggttt ttacaaccac aagtaacggt ttaatcgatg tgacaggtct aaaatatggc
     6421 acgtactatc tggaagaaac gaaagcgcca gaaaaatatg ttccattaac aaaccgtgta
     6481 gcatttacta tcgatgaaca atcttatgta acagcaggac agttgatttc tcctgaaaaa
     6541 ataccaaata aacacaaagg tacacttcct tcaacaggcg gtaagggaat ctatgtgtat
     6601 atcggtgcag gagtagtcct tctactgatt gctggactgt actttgctag acgcaagcac
     6661 agtcagattt agtctttttg caagaattgg aaaaaggagc agcggattac gcaaatgctc
     6721 ctttttcttt tattatcaaa cagtcatcac aaggagaaca caaatgaaac caaagaaaag
     6781 gaaacttcta gatctgctga tgcttttctt gcttctctca ggaataggga tactagctta
     6841 tccatttgtt agcgacgcgc tgaataatta tctggatcag cagatcatct ctcattatca
     6901 gcaacaagcg gtcaaagaaa atgaagaagt catggctaaa atccaagaaa acatgacaaa
     6961 gaaaaaccag caattagcaa aagaaggtgg aaatccaggt gcagatccct ttactaagaa
     7021 aaaagaacct gcgaaaaagg atagaaccta ttttgagaaa catacgatcg gtattttgac
     7081 gatccctgag atcaatgtcc atttgccaat ttttgatgaa acgaatactg tgctgctaga
     7141 aaaaggtgcc tcattattgg aaggaacgtc ttatcctacg gggggagaaa atacacatgc
     7201 cgttatttcc agccatcgag gactgcctca agcaaagttg ttcacagact taccgaaact
     7261 gactttaggt gattctttct atatcgaaat caatggacgg acactcgctt atcaagtcga
     7321 tcagatcaag acggtagaac cgactgatac aaaagatctg agaatcgaaa aagggcagga
     7381 tttcgtgaca ctcctaactt gtacacctta tatggtcaac agtcatcgct tacttgtcag
     7441 agggcaccgg gttccttatc atgcaaaaga agttcaaaaa gaagtacaga aagtatccga
     7501 gaacaaacga tttttcctgt atggattgtt agctgcatgt atactgattt tcctccttct
     7561 tatatatctt tttcgtcaat cgttcaaaat aagaaacaga agttagctat tcctcatttc
     7621 tataatatat ctattcgaat cgttccgtaa acaaacaggc tgaggtttga aaaagaaaac
     7681 ttcagcctgt ttttatgatg atatcaaaga gagcctatca aacactatct ttcttattac
     7741 taaatgagga tcttttctta agttttccga tattctttaa gaaggccttt ttgcatcaat
     7801 tcttgtttgt ttttcaagtt gcggatattc tcttcaaaat tttgagaata tatcactgag
     7861 aataacgtat ttaatacgta atcgaaggca atttgagagg agaaagtcgc tactttagca
     7921 aaatcatatt cctcctgcga tataacaaga cacatatcag cctgtttggc catttctgaa
     7981 tgctgatttc cggtaatcag caaggtaggg gcctctacat gcttcaaata ggtcattatt
     8041 cgttcataat gatggatgcg accgctgtag ctaataaaaa tggcgcaatc ctcgctagtc
     8101 aaattggctg cattccaaga agaatcactg tattcttcag ccatgatcaa aaacttattc
     8161 aacttgacca ttttattctg gaatttcctt gccgtgattt gagaatcacc ttttgcaaaa
     8221 agaaagattc gctgtgcctt gttcaatact ttaccgattt gatctaaatc ttcatgttca
     8281 atctgcaatt gtgctttttt tattgactgg atcgataaat ccgccatttt ctttgcaatt
     8341 tgctgagtgc tatcttcact tgaaaaagga aaattaggat caatcgtttg attgaactga
     8401 gcaatcaatt gatgaacttc ccgccccagc tcgaaacgga attctttaaa tccgctgaat
     8461 cccatttttt tgcataaacg aatgatagct gaatgggaag tatacgtagc ttttgctaat
     8521 tcttcaatcg tcatgtagac tgcagcttcg atattcctac ggatgtaatc gacaatccgt
     8581 ttctctgtcg tggtaaacaa ctcttgtttt gttagttttt cttcaataag catgatctga
     8641 cctccaaatt ctatcttact gataaacact tggttccgct actatctttt tgatttttat
     8701 ccttactttg aacattaatt tcaccaaacc gaatgataac aagtgttttt ctgaactttt
     8761 ttaccagaat agctattata aatactttct gcgcttttct aggatatact gaaaacatca
     8821 ataaaaggag gcaccactca tgcttacaat cgcttatctt ggcaatggaa agagtacaaa
     8881 tcgctatcat ttgccttttt caagaaaact tacgaacaaa atcaatatca aaaaaatttt
     8941 ttccccatct gaaccagtgt ggaaaacatt cgaagacatc gaatatactg atcagctgtc
     9001 tgatctttgg gatgatcctg agatccaatt agttgtcatc actacaccta gtcaatttca
     9061 ttatgaatat ggaaaaatgg cactcgaaca tggtaagaat gtactagtag agaaaccttt
     9121 tgcagaaacg tcaaaggaag cagaagagtt atttgcatta gcaaaagaaa aaggtctatt
     9181 tatccaatgt taccaaaaca gacggttcga ttctgacttc ttgacagtcc aaaaagtaat
     9241 cgaaagcgga aaattagggg atattttaga agtagaaatg cattatgatt atttcagacc
     9301 ggaagtacca gaaaatacgc atgaattttc tactgcgaac agctatttat atggacatgc
     9361 ttgtcataca gtagatcaag ttctttctta tttcggcgaa ccagatgata tcagctatga
     9421 tgtacgtcaa cttttaggga aaggacgaat gaatgattac ttcgatctgg attttttcta
     9481 tggaaatatg aaaatttcga taaaatccag ttatttccgc atcaaagaac gtccaagttt
     9541 cgtcgtttac ggaaaaaaag ggatgttcgt gaaacaaaca aaagatcgac aagaagaaca
     9601 tctgaaaatg ttttacatgc ctgatcatga agactttggt atcgatttgc ccgaacatta
     9661 tggtgtattg acctatatag atgaacaagg gagttatcat gaagaaaaag taatttccga
     9721 ggttggtgat tacagccgtg tctacgaagg gatttacgag acattagtta atggcgcaga
     9781 aaaagtcatc aaagatgaag aaacaatctt acaaatgaaa attttggaag aaggaatcaa
     9841 aagcattcaa gaatcagaag gaaaaatatg acatatttgt aagaatcggg tttgtttttt
     9901 gacgcataaa ctgtcttatt gtacgatgaa aaagtataga actactagga ggaaccaaca
     9961 tgacaaatta tgacggtgaa aaacacaaac gattaagagg ttcaatggcc cctaaaacag
    10021 aagaagaagt ggctttagca ggaactaatt ctggtgaaac agaagacaaa gaagaatcat
    10081 tagctggcac aaactctggt gaaacagaaa ataaagaaac agctttatct ggttcaaatt
    10141 ctgtagaaga ggaaacaaag cctaatgagc ccaatcactt tgaaaaagat atcgaaaaag
    10201 cggttgaaga tgtcaaagaa aaatttgaag aactgacctc aaaaaataaa catgatcatc
    10261 attgatcgga cgaaatcaat tcataaaagc aggagacatt tggtcacctg ctttttttct
    10321 gtatccattc gatagaaatc gttcggtaca gtcgtaaaaa aaatccaact gtaagttgta
    10381 ccaaacaccc ctcgatttaa aagcgctacc atactagaat ataagtagga ggtgaacagc
    10441 gctgtgaact acaaaattag ttatctcctg aaaacaccaa gagaaatcag tctagaaaaa
    10501 gcagcagaga tcatcgaagt aaatacaagc gaggcgatca aacaattaac tccttttgcc
    10561 gaatatgtgg atgatacccg tatccgtata aagaaattcc gagcaatcga ctgggcagaa
    10621 aaatttttgg atattactcg atttgaagct gtgtataccg aacaagagcg aagagatctt
    10681 ctttatttga tggtttattc gactgcaacg tctgtttctg tttttcattt tcaagatttt
    10741 cttcaggttt ctaaagggac agtcctgtca gatatcaaaa agataagaaa tgaattagaa
    10801 gtggaagatt gtcagctgtc gtatactagg aaaactggtt ttaggctgct agggtcagaa
    10861 gcaatattaa gaagagaagc aaaaaatcgc actgctcgtt tgatgcagtc acttgctgga
    10921 agatttgggc tcacgtattt aaccgcaacc ctttccattg attattacgc caaagttaga
    10981 gatttgctag ataaaacgat ttcactatca gatcttcgat tcgcaccaaa tcgaatggat
    11041 gaattagctt attatttgag tttgacaacc attcgaattg gaaaaacatc catggaactg
    11101 gtaaaagatg ctgaactgtt ggcttctctt gcagtcactg acattagtcg acaagtattg
    11161 actggccttg gctattccga acatcaaaat tcggagatta tatttttctc tctttgctta
    11221 gcatcgatcg ttgaaggaga tgtccaggag cctgcacttg attttttgct ggaatgttct
    11281 tcagaagtca tccatcgcat ggaattcctg atggccgtag aatttgcttc gtttcgtgaa
    11341 ttattgatga atgtctttta tcacttggtc cctgcctatt ttcgtatttc ctattctttt
    11401 tatttaccta atgtgatgat cgatcaaatt aagcatcaat atgcttccat ctacgaaatg
    11461 acaagaaaag cattacttcc gttagaaaga cgaataggga agagcatccc tgaagaagag
    11521 attggttttt tcactatact atttggcgga gagatcagaa aagcggacgc agaagaaaga
    11581 aatagaaaaa taagagccgt gatcgtctgt ccaagtggca ttagctcttc attgatctta
    11641 aaaagtgaac ttcagcaatt attcccgatg atctttttta cagagactaa ttcagctgac
    11701 cgcttacacg aggttgacga aacttcttac gatattattt tttctactgt acctctcaca
    11761 cttactgata agaaaaagtc tctctatgtt ctgcatccaa tcatgactga cttggaaaaa
    11821 aatcagctga tcaatcaagt acaaagagat tggctgatac caggattttc aatgccttct
    11881 gcccaagaat tgttgtcggc gctactgcct tatatcgaat taaaagaagg cgtagacgaa
    11941 gaaaagctct atcaagtatt aaatcgtaaa atgaacaaac ttttagaaaa gaaggaggat
    12001 caacgtccta tgctatctga attattaaca gaagaaatga tccaattatc ggatcaacca
    12061 aaaaactggc aagaagcaat cacatttgcc gcacagccgc tattatcaga aaataaaata
    12121 gaagaaagtt atatcaaagc tatgatcgag cgcgtggagc agtatggtgc gtttatccat
    12181 attggcgatc acatcgcatt acctcatgct cgtccagaag atggcgtaaa cgaagtaggg
    12241 atgtctttgc tgaaacttca acaacctgtg gatctgcttg ataatccagc ccatcccatt
    12301 agtttgttca tctgtttagc tgctatcgat aatgaggcac atcttcgggc tttggcaaat
    12361 ttaacaaagc tactttctaa taaacaaaat cttcaagatt tattacacgc atcaacaaaa
    12421 gaagaaatat tagcgatcat aagaaaagga gacgaatgat atgaaaattt ttgcagtatg
    12481 tcaaagtgga ttaggaacaa gttttatggt agagatgaac atcaaacaag cacttgcaga
    12541 tcttggagca gatgtggacg agtttgaagt aagtcatacg gatgttggaa gtgtatctag
    12601 cgatatggct gattatttct tcattgagca ttctttgaaa gatacgctaa cttctatacc
    12661 tgatgaaaag ctcgtacctt tgcaatcgat cattgacagc gaagaagtaa aagaaaaagt
    12721 aacgaagatc ttgtcaaaag aataaaaatg attcaaggga ggaactatca atggataaat
    12781 tgttgaattt gtttgtgtca atcgtcagtc agccctccat cctcgtttct ttgattgcac
    12841 tgcttggtct cgttcttcag aagaagaaat tttcagaagt gatccaagga actatcaaaa
    12901 catttgtcgg attcctagta ctgatcggcg gtgctggttt aatcagtaat tcactaaccc
    12961 cttttgcttc gatgttccaa acagctctgc atactcaagg agtagttcct agcaatgaag
    13021 cagtagtggc gattgctttg caacaatacg gtacacctac tgctttgatc atgctagtag
    13081 ggatgatcgt gaatatttta cttgctagat tcacaagata taaattcatt tttcttacag
    13141 gtcaagcaat gctttatgtt tcttgtctaa cagcagtcat tttagtaagc gcggggttta
    13201 ctgcaaattt gtggatgatc ttattaggcg gggtcttcga aggaacacta ttaactatta
    13261 cgcctgctct ttgtcaaccg tttatgaaga acatcactgg aaacaatcaa gttgcgttag
    13321 ggcataccgg aaatatcggt tatgcttcta gtggatggat cggaaagttt tttggaaaca
    13381 aagaccgttc tacagaggac ataaagattc ctaaaagttt cagctttttg cgagattcta
    13441 ctgtatcgat catgctcttg atgtcgatcg tatatgtcgc acttgctttg ttagctggtc
    13501 ctggatttgt tgaaagagaa ctaagcaatg gagacaacgc tgtaatcttt gcactgatcc
    13561 aagctggaac attcacagct gggtttgtta tcgtccttca aggggttcga atggtgttgg
    13621 cagaaatcgt tccagcattc caagggattg ctaaaaaact cgtacccgat tctaaaccag
    13681 cattagatgt tcctatcgtt tttccatatg cacctaatgc tgtattaatt ggcttttttg
    13741 ttagcttctt tgtaggaaca ctatccatgt ttgctatgat aggcttgcaa actacagtta
    13801 tcattcctgg ggtagtagga catttcttct gcggagctgc ttctggtgtc ttcggcaact
    13861 cgactggcgg tagaagaggg gcggtattag gttctgcttt caatagctta ttgattagtt
    13921 ggctgccatt atttatcctt ccagtattag gagatcttaa aatcgcttct tctacatttg
    13981 ccgatactga ttatctagta ccaggacttg tacttgggaa actaggagaa tacggtcaaa
    14041 tagccttgac agttggaatc atcgctttct ttttacttgt cgtattatct agctttcttt
    14101 ttgccggaaa aagaacaaac attgtttcta aagaacagaa ttaagcaaac ttcaaaataa
    14161 aaattgcttg gacaatatca gaagaagatt taaagtcttc tgatatgtcg caagcagttt
    14221 tttgttttat gcatttattt ttgaatagct tgttaagaca agtggctgtt ttgctagaag
    14281 aggataactt ttaccattca ttttcactgt tccgcctgtt ctatcagaag taatcacgat
    14341 cttatctttt gttttttcaa gcgaaatctt tccatctttg ataggtagcg tacaattcag
    14401 tgtttcaaat gaatctagat taggttgtat cataaattct tcataacctg gctttgttgg
    14461 atacaaaccg ataaaatagc gtcctaaaag atagattggg cttgctcccc atgcatggca
    14521 taagcttttt ccataaggat ctccatacat actgtacttt tcatttcctg tttgagtagg
    14581 gtcgtattct tcccagaaag ttaccgcatc atgagataac atccctcccc aataatcaag
    14641 aatcgtttgg tagacgatat tcgtctgtcc gagtttgcat aaagcatctt gttcatagaa
    14701 tttgaaatat ggagtagtaa tctgtgtgat agtgtcattc aataagacat tctttaagat
    14761 cgattgctgt tggtctttgt tagcaaaatc gaataaaaca gcgaaaatat tcgcatgacg
    14821 cgtcacattt cttctcccag attcatagca atcgataaaa gcgccaagtt cttcatccca
    14881 aaagtagtca aataggtttt gtttcaatag ttgatatttt tcttgataga gatgagtatg
    14941 ataattttcg ttctctccat atgtttcagc taagatatct ccgcaagtga tcatggaatg
    15001 ataggctttc agtaataaga cttgttctgc agcaagcgtt ccttctttat ccatttcaga
    15061 ccaatcaata aatatccaat cttttgctct cccataaata aaccctaatt cattcgtttg
    15121 ctccatcaag tatctcatta atgattgcat cttagggtag acgatgcgta aaaattcata
    15181 gtctttcgtc atcatatagt ggttttcgat accaatcaac cacaagatcg aatagtcaac
    15241 gatagtattc atatgctgct tgatttcatt ttgtcctctt aatgctaata tcgttcgttt
    15301 attgatttcc tcgtcaaaaa aaagatattg attgatcatg aatgattgat acgcatctcc
    15361 agaccaaatc cagcgatctc gtttgatacc gtcgataaaa aacaaatcac tacataactt
    15421 gaaggcttct tctgatactg accagatctg attcagtaaa gaagaatcgc ttgaaaaagc
    15481 agcttgtttc gaaagagata aaaattcatg aaaaacttga agttgaacca ttccagtaga
    15541 aacctctggt ataaacaaat agcgaaaagc gcgtttacga gctttcattt ttcttgtcat
    15601 tcctatttgc ttatagtagc aataatcgat atccatagct tctacatcgg actctccata
    15661 acatagagtg atagattcga tatggtcatc cagagatatt agttctacac agccattcac
    15721 tgctcgccca aaatcgaata agattcctcc gccaatctcc gtttcagtga ctggatggac
    15781 gcattctttt tgataataga tttgatttgg atcttttgcg atatcgacat aaagcggact
    15841 ccagccagct ggatattctt caataaaatt acttgctgtc cagccaatat ctgattttac
    15901 ttcgtctccc tcgataaaca tcgctggtag ccctgatgta tgtcctacaa agatacgaat
    15961 ttttgcttta ccagctggac aagtcagcca ttttccacat ggatgttttt gtccattaat
    16021 ttctacatac ccaactcctt ggatcgtcac tttaaacatg gaaggttgat caagaaagta
    16081 atatctctta aacttgacgt tccgatggca atcatccatg taccagtacg ctggccagtc
    16141 aaaacctcgt tcttcccgtt ggaaattttg tagcagtcca tggcggatct caaagtctcc
    16201 aggataccaa agccatctat ctttatgttt ttcatttgtc attttacacg acctccatca
    16261 aatttatttt gttgtcaatt gttcttttat ttcttcatct actcttatca ctgtctttct
    16321 attcgtcctg tgtactacta gaaggctaat aagcgcgcta atcaacagca agatactgcc
    16381 attgacgaaa acagcaagtg ttttactccc aagagaagca aaagagagaa agagcaaacc
    16441 atatggtgca atgaaagtac ctaaattagc gccaaccaat aagattgaag tattgaaact
    16501 ctccgcgtac ttgggcgctt ggatacttac ttggttgaat aagtaaggaa tgaataacga
    16561 aaaagaaatt cctgttagga tcgcaccaaa cccaatcaca tacaggttta tcacagtggc
    16621 aatcaataca aagccgattc ccatcagtag taaagaaagt gtaagcatat agcgatgtgc
    16681 ttttgtctga agccagccaa aacaaactcc ggcagccata cttgccaatt gcatgattgt
    16741 taagatattc gctgcattag tttcgtttcc tagagaataa gtcgtcagga ctgtggctaa
    16801 tttaatcgat acagcattat ataccacgat gaccagcaag agaaaaatac cataactaat
    16861 cattttagga ttcgttttga ttacttgtct tgttggggct gtgccatgcg attgaagttc
    16921 agttgtttca ggtgtaggaa caaacaaaac aaacaagaca agaataggta agctgatggc
    16981 gtagactaaa aaggtatatt gccaaccgaa ctgtactagc tgtcctgcaa caaatgtcat
    17041 taaagctgca ccaaatcctt gaaatgcact ttgaaaacca atcatagagg ctcgttcatc
    17101 tccgtgataa aaggcagcaa tcatgctgac tgccaaagaa ttgatcaatc caaagccaat
    17161 acctagtcca gctcttgaag ctaagacaat actgtaactt tgtgagaaga gggggaggat
    17221 accacaaatc gatgcaatca ctaatccgat gatcacagtt tgtttttgtc cgattttcct
    17281 agcaataaaa ctactaatca aaacaaaaat agtgaccata aaagagggag cagtcgttaa
    17341 taattcaaca gcagataaag aacgcgtggc ataagtagct gacatactgg gaattgcaga
    17401 cgtgactgca ccagcagaag tcaaaataag agagactgac agcagagaaa atttcatcat
    17461 ctttttattc ataagcatgt tcctttcatt tttgtaagcg cttatcaaaa atatagtatg
    17521 aagtggttat tctctcaata gcggaatcac aggaagttca tgtcccaacc atagaaagtc
    17581 tttatgaaac caaaaatagc tttgcatata atagcagtac agcgtgaact aataagcgaa
    17641 agaaaccgct tacaaaaagg agggagtcaa ttggaactag ctgaattaga catttttttt
    17701 gcgtaacgaa gaatcaatcg aaactctgca gcgtgaacaa ggaatgaata tcaatgattt
    17761 aaacttagct taccatcttc agacaccgcc aaaaatccac cgaggggatc tatttcaaca
    17821 gggaaatatt catatcagta aacaccgtcg atatgcggaa gttcccgagc atacacatga
    17881 ttttgttgaa atcaattata tgtatagcgg tacatgttta aaaattatta atggagaaag
    17941 gatagagatg gaagaaagtg acgtggtgat cattgataaa gaagctactc aaagaatcga
    18001 atacacaggg caagaggata ttttaatcaa catcttatta aaagaagaaa ccttatctac
    18061 agagatattg aatcatttgg caagcacaaa taatctactt tcttcttttt tgataaatgc
    18121 atcaaaagaa aaaggggaac atacacaatt cattcatttt cgcggcggaa aaacaagtcg
    18181 tttgacgcat ttgattgaaa atatgctgtt gatttatttt gatacataca gtcataagat
    18241 ccgttcgtta aatctctata catcactatt actgatcgaa ttgactcatt tattagaaca
    18301 acaaagcttt tcatcagcag atggatgcgc agaaaatgag atgcttagag tattagatta
    18361 tatcgataag cattttactt cttgtacgtt aaaagaaaca gcagctcatt ttggctacaa
    18421 tccggtctat cttagtaata aattgaaaat gtattttgat gcttcctttc aagaactggt
    18481 tctaaagaaa aaattcgact ttgcgttgga actgattcaa gaaacgagtt tttcgttgga
    18541 agaaatcgct aatcgtatcg gatataaaca aacgacttct ttattcaaat tattcaagaa
    18601 gtattcaaca tgtactccca atgaatggag aaataaacat taagaagaaa atctttcact
    18661 aaagcatatg aaggaaaatt gttttaggtg cgattgctgt cttattatat cgttgcagcg
    18721 aatttttttg gatttatttt cagttctaaa cttttggttg tattatcagg ggatataggt
    18781 gtaaacattt gtcatttatc tgttatgcgt attcaggaat tcgtttccat caaaaattat
    18841 aataacaact attataatta acatgttgat aaaatgaata aacaagacta tacttttcat
    18901 gaaagtaaca aatagttatg aaaaggagct gtttattcat gaaaaaaaca agtcgtaaag
    18961 tagtaatcgt cggtacaggt tttgttggaa caagtatcgc ttatgcaatg atcaaccaag
    19021 gaatctctaa tgaattagta ttgatcgatg tgaatcaaga aaaagcagaa ggggaagcat
    19081 tggacctttt agatggtatg gcttggggcg atgaaaatgt ggctgtatgg tcaggtggct
    19141 atgaagaatg taaagatgct gatatcgttg ttatcacagc tggtatcaac caaaaacctg
    19201 gtcaatctcg tttagattta gttaaaacaa atgcgtcaat catgcgccaa atcgtaaaag
    19261 aaatcatggg atcaggattc gacggtatta tcgttgttgc ttcaaaccca gtagatattt
    19321 tgacatatat cgcttggaac gaatctggtc tgccaacatc acgtgttatc ggtactggaa
    19381 caacattgga tacaactcgt ttccgtaaag aaatcgcatt gaaattaaaa gttgacccac
    19441 gcagcgtcca cggctatatc ttaggtgaac atggtgattc agaagttgct gcatggtcac
    19501 atacaactgt cggtggtaaa ccagtgtttg aaattgtaga aaaagaccat cgtattgccc
    19561 aagatgaatt agatgtgatt gctgataaag tccgtaatgc tgcttatgag atcattgatc
    19621 gtaaaaaagc aacttactat ggtattggta tgagtactgc acgtatcgta aaagctatct
    19681 tgaataacga acaagctgta ttgcctgtat ctgcttattt aactggtgaa tacgacgaaa
    19741 aagatatctt tacaggtgtg ccatcaatcg ttgacgaaaa tggtgttcgt gaagttgttg
    19801 aattatcaat caacgaagaa gaaaaagcaa tgttcagcaa atcaacaagt gctttaagag
    19861 aagtattgaa cactgtatta taagaaacaa tgaatatgaa ctaaaaatcc taaaactgct
    19921 taacagctga aatagctgtt aagcagtttt ttattgatat ctaataggta agcaaataaa
    19981 tagaatggtt aaaattaatc ggtccgaaaa tgtatccgta catatgaaca tatgaaattt
    20041 aatctgttga ttacaataag cgaatcgtta tcaatgttta tctgtggtta attaaaaaat
    20101 ccttccgcaa cggcaaatac aatgattcat atctaattcg ttcatatttg attaactgaa
    20161 taaagaaact ggtttttatc attcaattaa acgacaaaaa aagtaagaaa aactgcaaaa
    20221 tttttttata attttgcata agcagatttt gatggaaaaa tgacgatttt tgataaaaaa
    20281 ccgttcgaga gaggtt
//
LOCUS       DAYYME010000043        20253 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000043 DAYYME010000000
VERSION     DAYYME010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.043"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(249..1274)
                     /locus_tag="ACHBFW_001290"
     CDS             complement(249..1274)
                     /locus_tag="ACHBFW_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9317603.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1549..3234
                     /locus_tag="ACHBFW_001291"
     CDS             1549..3234
                     /locus_tag="ACHBFW_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM9317604.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3248..4531
                     /locus_tag="ACHBFW_001292"
     CDS             3248..4531
                     /locus_tag="ACHBFW_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9317605.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4601..5470
                     /locus_tag="ACHBFW_001293"
     CDS             4601..5470
                     /locus_tag="ACHBFW_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9317606.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5467..6303
                     /locus_tag="ACHBFW_001294"
     CDS             5467..6303
                     /locus_tag="ACHBFW_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9317607.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6329..7396
                     /locus_tag="ACHBFW_001295"
     CDS             6329..7396
                     /locus_tag="ACHBFW_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFM9317608.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7393..8220
                     /locus_tag="ACHBFW_001296"
     CDS             7393..8220
                     /locus_tag="ACHBFW_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM9317609.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8265..9290
                     /locus_tag="ACHBFW_001297"
     CDS             8265..9290
                     /locus_tag="ACHBFW_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9317610.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9287..11470
                     /locus_tag="ACHBFW_001298"
     CDS             9287..11470
                     /locus_tag="ACHBFW_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFM9317611.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11470..12600
                     /locus_tag="ACHBFW_001299"
     CDS             11470..12600
                     /locus_tag="ACHBFW_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM9317612.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12597..13274
                     /gene="pgmB"
                     /locus_tag="ACHBFW_001300"
     CDS             12597..13274
                     /gene="pgmB"
                     /locus_tag="ACHBFW_001300"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM9317613.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13358..13885
                     /gene="msrA"
                     /locus_tag="ACHBFW_001301"
     CDS             13358..13885
                     /gene="msrA"
                     /locus_tag="ACHBFW_001301"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM9317614.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14086..14772)
                     /locus_tag="ACHBFW_001302"
     CDS             complement(14086..14772)
                     /locus_tag="ACHBFW_001302"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFM9317615.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            14852..16390
                     /locus_tag="ACHBFW_001303"
     CDS             14852..16390
                     /locus_tag="ACHBFW_001303"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFM9317616.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16510..16977
                     /locus_tag="ACHBFW_001304"
     CDS             16510..16977
                     /locus_tag="ACHBFW_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFM9317617.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17511..17939
                     /gene="rplM"
                     /locus_tag="ACHBFW_001305"
     CDS             17511..17939
                     /gene="rplM"
                     /locus_tag="ACHBFW_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFM9317618.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            17955..18347
                     /gene="rpsI"
                     /locus_tag="ACHBFW_001306"
     CDS             17955..18347
                     /gene="rpsI"
                     /locus_tag="ACHBFW_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM9317619.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18435..19661)
                     /locus_tag="ACHBFW_001307"
     CDS             complement(18435..19661)
                     /locus_tag="ACHBFW_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9317620.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(19733..20035)
                     /locus_tag="ACHBFW_001308"
     CDS             complement(19733..20035)
                     /locus_tag="ACHBFW_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9317621.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
BASE COUNT         6526 a         3656 c         4240 g         5831 t
ORIGIN      
        1 aagggtaaat gtgtttaaaa aaacgaacta taaaaactta tacacaacgt ggttaaaaat
       61 agttttttct tccctgtgga aaaaatagtt taacacaaac attatagtta tccacagcta
      121 tctaaaacag atggaaaata agaaaaattc gctaaaatac cgggaggggg taacgaggga
      181 tgagtgattg aaaaaagaac agagtaactg acttatcctc agtgtttctg ttcttttata
      241 attctgttct acatttcttg ttgttggatc gaatctgtga gagtaatctc ggttgaaaga
      301 atgattcttt tttcttcagg gtattggtgg tggattagtt ggaataaaag ttctgtggct
      361 ttgattccta actgatttgt cggaatgtct atccgagtca actgtggcga aaatagggag
      421 gacaataagg gatcactgaa tcctgcgatc gaaagatcat caggtatctg ctttccagat
      481 tgttctgcca ctttgtaaat actcaatgat tttaatgcat cggtagaaaa aatcgcagtt
      541 ggtctttctc gtagcttcat taaatcattc attgctgcca tgctatcttc taagcgaaag
      601 ccgccgtcta taatccattc attacggata tctaggtggt gatcaaataa gcattgacgg
      661 taaccattga gacgatcatt ggatacataa acttctgtcg gagcatggag acagccgata
      721 tgacggtgtc cattcttgat cagtgtttcc accaaatcgt aactatcctg gaaattatcc
      781 gtgtccactg agtagatgtt cgtgtagtcg tttccgactt ttccgattac cacaatagga
      841 atattgtatt gatccaattc aaaaagaagt gtttcatcca tcgtagagct taaaataata
      901 attcctcgga tcattttttc ttggatcttt ttcgcgcatt tttctagctc ttcttctggt
      961 gtttttgccg tttgtagaat caagtcgaag ttattttgtt cagaaacggc agaaatggat
     1021 tgaataacat ctgaaaaaaa aggattgcca taggtcgtat gagaagtacg atgagataat
     1081 gctagaatcg cgtcaaaacc atttcctgac agtcctcttg caattttgct cggttcataa
     1141 ttcaattctt tgattgcttg aagtacactt gcttttgctt tatcagagat atttggctgg
     1201 ttatttaata ctctggagac agttgatttt gagacattag cttttttcgc aatgtcataa
     1261 atagtaggtt tcaattttat atgcacctcc tttttgggag cgttttagaa taattccata
     1321 gtacaataga cattcttttt tgtctatgat tttgaaaatt taggaatagc ttgcatgatc
     1381 atgagagacc atattttttt aagaagctat taattattga tacataagct ttcttatttg
     1441 tatttttgtg tatatcaaaa ttatttaaaa aaatgctatt gacagtaagc gttttccata
     1501 atatgatcaa atcgttggga acgctcccaa aaaagaaagg ggaatccgat gtatacaaaa
     1561 cttttacaag cgatttatcg cgactcagat ttgtctgaga aaaccgcctc tatgattgaa
     1621 caaaaagtca gtgtttatca gcagaagaaa ggccaggcag gcaaactgca ttgggacgaa
     1681 gaagatattt tcttgattac ttatggggat caattttttg aagagcagca accaactttg
     1741 cgtacattta agaaatttta tcaaaagtat ttggcagata catttagagt tgtacatttc
     1801 ttgccatttt tcccatatac atctgatgat ggtttctccg ttgtaaatta tcaggcaatc
     1861 aacgaccgtt tgggcacatg ggaagatata gaagaaatga gaagcgaaac acggttaatg
     1921 ttcgactttg tctgcaatca tatgtctgcc gaaagtactt ggtttcagga atatttaaac
     1981 ttgaatgata cgtacaaaga tttttttatt gatttgccac tggatacaga tacaagcagc
     2041 gtgactagac caaggaccag cccgttactt acaacgttca ttgacaaaaa cgggaaagaa
     2101 aaacacgtat ggacgacttt tagcgaagat caagtagact tgaattttaa aaatccagaa
     2161 gttttattga aaatgatcga tattcttttg ttttacttag aaaaaggcgc ttcatttatt
     2221 cgcttggacg ctgtgggatt tttatggaaa gaactaggaa cttcttgtat acacctgcca
     2281 gaaacgcata aaatcatcca attattccgt gcaatcgctg atgaagcagc tccaggaacg
     2341 gtattgatca ctgagacaaa tgtgccacac aaagataata tcagttattt tgggaatggc
     2401 gagaatgaag cacagatggt ctatcagttt tcgctaccac ctttgaccct tcaagcgatt
     2461 cgttcgggta atacaagtaa gttgaaagaa tggttaaaaa ccatcgattt cgcatcgaaa
     2521 aaaacaactt tttttaattt tttagcctcc catgacggta tcggattgaa tccggtacga
     2581 ggtattctgc cggaaaaaac gatccttgaa ttagtggaac agctgaagaa agagggtacc
     2641 cgagtaaact acaagaaaaa cccagatgga acgaccagtc catatgagat aaacgctacg
     2701 tatctagatg cgttaagttc tccaacagat acgacggaat ggagaagcaa acgcttttta
     2761 gctgctcatt catttttggt aacgttacca ggcgtgccgg caatttatat tcaaagtgtg
     2821 ctgggaagtc gaaatgatta caaaggcgta gaaaaaacag gagagaatcg aaaaatcaat
     2881 cggcaaaaat ttttgttagc agatattgag aaagagttgc agacagatcc attaagaaca
     2941 gcagtatatg gtgaattgac ccagatgcta tctattcgaa aaaaagaaaa agccttccat
     3001 ccgaatagtt tgatttactt actggactca gcagattctt ttatcagttt tattcgcgaa
     3061 gcgaaggaag agaaagtctt agttatccat aatttttcca atcaatcagc aggttatctt
     3121 ttaccaaaag gagattacct agacctgttt tctggacaaa ttatccattg cagtcaagaa
     3181 cgaaagttga tattccagcc gtatgaatat atgtggctga aaataataaa ataagaggtg
     3241 gaaaaaaatg aaaaaaatta ttgttactac ctgtctgcta gctactggat ttcttcttac
     3301 tgcttgtggt gaaagtcaaa gctctaacga atcacaacag acaacgattc aattcatgca
     3361 ctcttctgtc gaacaggaac gattaagtgt gatcaatgaa ctggttgctg attttgaaaa
     3421 agaaaatcca gacatcaaaa tcaagcaagt gccggtagaa gaatcagcgt tcaatacaaa
     3481 aatcgttacg ttagctcgtt ccggtaaatt accagaagta atggaagtca gtcaggattt
     3541 tgctaaagtg atggataaag atgaactgat tgatcagacg gcagtccaaa acgttatcga
     3601 ggaagctgga gaagataatt attatgacgg agccaaaaac cttgttcgtt cagaagatgg
     3661 aaaaagctat attgctgcac caattagcgg ctgggttcaa ggtatttggt atgacaaaga
     3721 aaaattatca gaagctggtt tttcagaacc agaaaattgg gatgatattt taaaaattgc
     3781 tcaacatttc acagataaag agaataaaca atatggaatt gctatgccta ctgctgaagg
     3841 aacaatgagt gagcaagcgt tctctcaatt tgctttgtca aatggagcaa atgttttgga
     3901 tgatgaaggc gaggtaacga tcgatacgga aaagatgaga gaagcattgt cgttttatca
     3961 aaacttatcc caatacacga tgccaggctc taatgatgtg acagaaatca aagatgcttt
     4021 catgaatgga acagcaccaa tggcaatcta ttcaacttac attttgcctt ctgtttatga
     4081 agcggggaac tcagaaaata tcggttttgc tatcccaaca caaaaagatc aagctgtcta
     4141 tggcacagta tctggattga cgatttcttc tggtctagac gagaaacaaa aagaggctgc
     4201 tgaagcattc acagctttct tatctcaacc gaaaaacatg gaaaagtggg tcttgatgtc
     4261 tcctggtggt gctcaaccag tgaataaaca agtagtcgaa ctggatggtt acaaagaaaa
     4321 tgaagtcatc aagtcttttg gcgagctgcc atctgagatt gctagtgctt ttgatgaagt
     4381 acaagtcttt ggactagtcg gggaaaagaa tttcacgaaa atgggagata tcacaagctc
     4441 aggtgtgatc ggaaaagcag tgaaccaagt aactgtcgga aacgaagatg tcgatcaagc
     4501 tttagctgac gcacaaaaaa atacgaaata acaagaagtt ctgccaatgg tgttcgaaaa
     4561 aagtagatag ctattggcag attatcaggg ggaggtttca atgaatcgtc caaggaaaca
     4621 ttctgactta aaattagcca tgggactttt agcaccaagc atgcttttat tagctgtttt
     4681 agtgctgatt ccaatgcttt ccaatattca gatcagtttt ttcgatatgc cgctgaatcc
     4741 taactcagac gccgttttta tcggattaag caattatctt gaaattttaa gtgatagtga
     4801 attttatcgt tctttaggtt taacagttgc ttacacattc ttagttgtag caggcagtac
     4861 agctttagga ttagccgtag ctattttgct taaccgcaag ttcagattta ggaaaacggt
     4921 gcgttcactg attttattaa gttatgttac gccatctatt tctttgattt ttgcttggaa
     4981 atatatgttc aataatagtt atggaatcgt caatgatctg ttagtagata ggttgcagct
     5041 tttcaacgaa gcaccgctgt ggttcgacca gccagttgct agcttcttta tggtcgtttt
     5101 gtttgctatc tggagatact atccgtatgc gtttatttca tttttggcta ttttgcaaac
     5161 agtcgacaaa acgctttatg aagcagctga aatggacggt gcaaatgctt ggcagaagtt
     5221 caagatcgtg acactgccgg caatcaaacc agtactagca actgtgattg ttttacgatc
     5281 tatctggatg ttctatatgt ttacagatgt ctatctactg acgaataaag tcaatatctt
     5341 aggcgtttat ttgtatcaga cagcctttgc gttcaatgat cttggaaaag cagcagctat
     5401 atcgattcta ttgttcatca ttattgcagc ggtcgtcttg ctctcacgaa agaaggtgga
     5461 cttgaaatga aaaataaaaa gagcaggaaa atcttatttt acgcgacgat tttgttgcta
     5521 ttagctgtca atttgtttcc atttttgatc atgctgatga catcattcaa ggattcacaa
     5581 gaagcaattg caacgacaca aactattttt ccgaaagaat ggacttttga acattatcag
     5641 gatattttca atgctgagat ttttccattc atcatgtatt tcaaaaatag tttagtgatc
     5701 tccttgatag cctccgtaac tgcagtagtg attgggatca gcggggcata cgctttgtca
     5761 aaactacgct tcaaagctcg ctcgaaaatc aatgctagct tttacaccgt ttatatgttt
     5821 tccggtattt tgatcgttgt tccattattt aaaatcattt ccggtctagg tttgtatgat
     5881 accaatacag cactgattat cacgatggtc gttcaaacac ttcctactgc gatattcatg
     5941 ctaaaaagtt attttgacac gattccgact gatattgaag aagcagcaat gatcgatggt
     6001 cttagccgga tccagatcat tattcggatc attataccat tggctatttc tggaatcatt
     6061 tctgtcttcg tttattgctt tatggtttcg tggaatgatt atttatttgc ttcgattttc
     6121 ctgtcgacct ccgaaaaatt tactttacca atcggtttga acacattatt cagtacacca
     6181 gactacattt ggggacgaat gatggccgct tcattggtga ccgctttacc agtcatcatt
     6241 atgtatgcgt tatctgaacg atttattaaa ggtgatttag tcgaaggcgg cgtaaaagga
     6301 taaaaagaaa agaaagagga aagaaaagat gaaaaaatta gttgcattag aaccaagaat
     6361 agccggattc aaagattacg aagaccgtga gatcgaaaag aacgaagtac gtatccgagt
     6421 aattttcgct actccaaaac caggaacaga aattgtcgac tttagaggat taagtccatt
     6481 tatcgatgaa gaattttcag aagaatggcg cctattcttg ccaagagaag aaggaagcca
     6541 acgcggtgtg gtatttggcg aatttcaatt aggaaatatg attgtcggta cgatcgaaga
     6601 aacgggcagt ttggtgacag agtatcaaaa aggagagtta gtctgcactt atggacccat
     6661 tcgtgaaacg ctgattacaa acggtgttga taattataaa ctacgtaaac ttgatcggtt
     6721 ggatcaatgg aaaaatgcag tatgttacga tcctgctcaa ttcgctttag gtggtatccg
     6781 ggaagcacat gttcgagcag gtgatgctgt agcaatcttc ggtttaggcg ctatcgggca
     6841 aattgcgatt caattagcta aaaaagcagg tgctactacc atcgttggga tcgatcccat
     6901 agcgcagcgt agagaattag cagaaaaata cggtgccgat tacacgctag atcctatgaa
     6961 agcagatgtc ggttttcgtc taaaggaatt gacagataaa cgtggagtag attcagttat
     7021 tgaaacgagt ggcagtgctg ccgcattaca agcttcttta agagggatcg gctatggcgg
     7081 gatcatttct tatgttgcct ttgctaaacc ttttccagct gggtttaatc ttggtcgcga
     7141 agcacatttc aataatgcaa aaatcgtctt tgctcgagca tctagtgaac caaacccaga
     7201 ttacccaaga tggaaccgta aacgaatcga ggacacatgt tgggaattat tgatgaacgg
     7261 ttacttggat tgtgaagaaa tcattgatcc agttgtgcca tttgcagaaa gcgcagaagc
     7321 ttacatgcgt tatgtagacc agcacccaga agaaagtatc aaaatgggcg ttcaagtccg
     7381 aggaggagaa caatgaaatt cggtacgcaa gatcaagcat ttttcccaag agacatttta
     7441 gaaaaattta gatttatcaa agactgtgga tttgaagaat acgaaatcga cggtaagttg
     7501 ttaatagaaa atatcgacac agtcaaacag gcaatcaaag aaacagggat cccagtagtg
     7561 acagcttgta acggctacga tgggtggatc ggtgacttta tcgaagaacg gcgtcttaat
     7621 ggtgtacgtc aaattgctga gattttgcgt gcactaagcg aggttggagg caaaggaatc
     7681 gttatcccag cagcttgggg aatgttcact tatcgattac cgccgatggt ttcaccccga
     7741 agcaaagaag gagactataa agcagtcagc gaatcacttc gttatttaga taaagtagcc
     7801 gaagaaacag gtacggttat ttatttggag cctttgaacc gataccaaga ccatatgatc
     7861 aatttgttag cggatgcgcg tcattacatt gactctaatc aattgaaaca tgtgcagatc
     7921 atcgctgatt tttatcatat gaatattgaa gaagatgata tttcaaaagc attgactacg
     7981 caaaaagagt atgtgggtca tatccattta gcagataatc atcgctacca accaggatct
     8041 ggcgcaattg attttactcg ccattttcaa acgctacttc aaaatgagta tgaaggttat
     8101 atggtatatg aatgtcgttt gcgaggagaa aatgaagtga aaatttatca ggaatcattg
     8161 caatacttga aaaactgtct ggaaacagcg cgtcatttaa atgatttaga gaaagaatag
     8221 agaaagaata acaataagta aataagcaaa ggagggagaa ataaatgaag aaagtagcaa
     8281 tcataggcgc tggacaagta gcagaaaaag tacatgctgc ttattatcaa aaaagatcag
     8341 atgtcttaga attggccgct gtggttgatc cagtcagcca gcgagcagaa gacttttgcc
     8401 gacgtacagg attttctcat gcgtatacat cagttacaca aatgctgaat gagatttctc
     8461 ccgatatcgt gagtgtctgt acacccaatc gctttcactt cgaacatgta ttggctgccc
     8521 tcgaagctaa tgcttcagtg atgtgcgaga aaccaccagc tatgacagct gatcaagcaa
     8581 gaaagatggc agagacagcc gataaaaacg ggcagatttt ggcttatgga ttccagcatc
     8641 gtttttctga cgaagcaaca atgatcaaga atcatcttga tgttctcggg gatatttatt
     8701 acgtagaagc gaatgcgctt cgtcggtctg gtgttccagg ctggggaacg tttatcgaca
     8761 aagaacttca aggaggcggg cctttgatcg attatggcat tcacatgctt gatacagcga
     8821 tgtatattac tggatttcct aaagtaaaaa gcgttcgggc gtacagcttc caaaaaattg
     8881 gtacgaaaaa aaattcaggg aaattcggga aatgggatcc aagcaaattc acagtagaag
     8941 atgcaatttt tggcacactg gaattagaaa acggcaagat catccaactg aatacttcgt
     9001 ttgctttgaa tatcagagaa gacaaactga atgtggcttt ttgtggcgat caggcaggtg
     9061 ctaatctgta tccaggcgaa ctattcgaag atgtagatgg aaagctggtt tccttacaaa
     9121 agaccacgcc agttaatgac ccacatgaac taagtatggc tcattttgtg aatcgggcgc
     9181 tcggtgacca gactcagatg attgcagatg gctggcaagg atatgtcatc cagaatctgg
     9241 tggaaaattt atatcgttcg gcagaaacag gagaagttat taccttatga aagtagaaga
     9301 aaaacagttt gatttagagt atatcaataa gtatgcgagt cagatgacca ttggaaatgg
     9361 attttttggt atcagaggaa gccaggagga agactatcgg aatcaagtaa ggggaatgtt
     9421 tgcagctggt gtatataacc gacctttagg agcagaatca gccgagttag tgaatctccc
     9481 agatgtcatc agatgggaaa taaaaatcga cggtgaacat ttttctcttg agacgatgaa
     9541 agttcttgca tacgagcggg cactagattt agaaatggga gagcttaaac gctatgcgct
     9601 cattgaaact gctcaaggaa aaaaagtgga agtcattact tgtcgtgttg ctgatcaaaa
     9661 taatctccat caggcagccc tgaaagtggt catcaagccg ttgaccgaag actgccaagt
     9721 agatgtgaaa acgggtatcg atggacagca aaccaatttt ggtacacagc agttggttga
     9781 aaaagaactg cgagtatttg gtgacgattt gatcagtgca tcctaccaga caacagaaag
     9841 taaaattgat attgggattg cagctaaatt caataaagca ggtgtattcc aagcaaaaaa
     9901 tcgccggatc accgcttcct tttcagaaaa gatcagaaaa aatacgtcct ttacgctgga
     9961 gaagatcatc gtgctctcta cctctcttca gcaaacagac ccgtttaaga aaagtattgc
    10021 ccaagcccag aaaagcgtta gttatacaga aattttagag cagtcgatgt cttattggac
    10081 agatttttgg catcagcatc ggataacgat ccaaggcgat tcttctttcg atcagttagc
    10141 gatggacttc gctgcttacc atttacagat tatgactccc cgcaatgatc caagtgttag
    10201 tgtaggggcg aagggactaa ctggcgaagg ttataaaggg catgttttct gggatacgga
    10261 aatttttatc ctgcctttct ttttatatca gtttcctgaa gtagcaaaga atttgctgct
    10321 gtaccgttat gatcgattag aaggcgcacg gcaaaaagca agagattatc actatcaagg
    10381 tgcacttttc ccatgggaaa gcgcacacac tggtcaggaa gagacaccca aatttgctgc
    10441 attgaatatc aaaaccggaa caagacaaga agttgcttca gctgccgcag aacatcatat
    10501 cagtgcagac cttgcttatg ccgttcaaga gtatgtccaa gcaacagagg atcaatcgtt
    10561 tttagaaaca aagggaaaaa cgctcattca tgaaacagca gctttttggc tgagcagagc
    10621 tgtaaaagtc gatgatcatt tagagataca cagagtcatt ggtccagatg aatacacaga
    10681 atatatcgac aataatgcat atacgaacta tctggcgcat tataatgtgg ccttagctgt
    10741 taaatatcaa gctggcgatc cgcttttttt gaaagaatgt caaaattttt tagataagtt
    10801 atatttgcca gtgcctaaca aagaacagct gattccgcaa gatgacacgt atctcagtaa
    10861 gccagttatt gatttgacca cttacaaaga acgtcctggc agccagtcta ttttacttga
    10921 ttatgcccgt agcgaagtaa atgaactcca agtcagcaag caagcagatc ttgtcatgct
    10981 gttatactta ttacctgagt atttttctca accaatcgtt tctaagaatt tgcattacta
    11041 tgaaaacaaa acaatccacg actcatcgtt gagtaaagcg attcacgcca tagaggcttg
    11101 ccgtatccaa gaaatggaat gggcgtatca tttattccaa gaagcgtgcc tgattgattt
    11161 aggaccagat ccgcattcat cagatgacgg aattcatgct gcttcattag gagcactatg
    11221 gctagcagtt atatttggtt tttcaggtat tacagtaacg aatggaactc tttctatttc
    11281 tccaagactt ccttcagcat gggaaaaaat ttcttttcct tttatttgga aaaatcacag
    11341 gttgctattt gttttgacaa aaaaacaaat agtaataaaa aaagagacga aagaaacatt
    11401 acctattttt gtcaatggag accaatacca gctggaaaaa gaactgatct tggaacggag
    11461 gggaaaataa tgcgtattct gattgcgcca gattctttca aagaaagtct gacagcctta
    11521 gaggcagcac aagccattca atcaggtttt caagaagttt ttgttcaggc tgaatatgat
    11581 ctgctaccta taggcgatgg aggagaagga acggtttcat cattagcaga cagtttaagg
    11641 ttgaagcggc aaacagcagt cgttactaat gcatttggca aaaacaaaga aatccagtac
    11701 gcttcagacg gtcaaactgc gatttttgaa atggcagaaa tcgtcggatt gaaagatgtc
    11761 ccactaaacc aacaacagcc acttaggtta tccacgcgag gagtaggaga actgatacga
    11821 caattgttgc aacaaggtgt tcgacacatc atcatcggcg ttggcggaag tagtaccaac
    11881 gatggcggca tcggcatggc agaaggtcta ggttatgctt tttacgatca agataatcaa
    11941 aaagtagaag cactgggaga aaatctaggt aaaattgcta cagttgataa aacgaacgtg
    12001 cctattgagc tgattaaagc caaaatcact gtcattactg atgttactaa tcttttatgc
    12061 ggaaaagaag gcgcttcttt tgttttcgga ccacaaaaag gcttgaaaga aaatgaatta
    12121 gcagtggctg accaagccat gcatgatttt tatatgagat ttgcccctca agtaatcgag
    12181 gcatcgggca gtagtgctgg tggtggaatg ggtgcaggac tgttagcatt tgcttcaggg
    12241 aaaatagtct ctggcatcga ctacgtattg gatttactgg agttcgataa acgggtacag
    12301 caagcagacc tcgtaatcgt aggcgaagga cgtctagatg gacaaagtct aaaaggaaaa
    12361 gcgccagttg ggattgctag aagagtacca gagggcgttc ctgtgatagc tatttgcgga
    12421 agtgttggta aaggaagcga aaaagcagca gatgttggca tttcggccgt ttttccgatc
    12481 cttcctgcag ctgtgccttt acaagaagct ttacagcaag ccactgataa tcttcatcgc
    12541 acagctaaaa acatcgcatc tttacttcaa gtcgttcatt ttcaaggagg taatcgatga
    12601 aaatcaaagc agcactattc gatttagatg gcgttcttgt agacacagct cgttatcatt
    12661 acgaagcgtg gctggtttta gctaaccagc tgtccattcc attcactgag aaagaaaacg
    12721 agcaactgaa aggaatatcc agaaccgagt cactagagcg attgctgtca tttggaaaaa
    12781 tggaacagaa attctcagaa aaagagaaat cagcctttgc tgagcaaaaa aacaatctat
    12841 atcttcaagc gattcaaaaa atggatgaaa catccgtttt gcctggtgca atagctgttc
    12901 ttgagtactt gaaaaaaaca aacatcaaga tcggattggg atcagcaagc aaaaatgcac
    12961 gcctcatttt agaaaaaacg aatttgacaa gttattttga tgtccttatt gacggcacgc
    13021 aagtatcaaa agcaaaacct gatccagaag tttttctcaa aggagcacag caattgaatg
    13081 taccgccaaa tgcatgcttg gtcattgaag attcagaagc tggttgtcaa gcagctttgg
    13141 ctggtaacat gcatgtattg ggaataggtg aaaacataaa tcttccttct gctgaatatg
    13201 tgattcctga tttaaccgtt tttgatcaag tacgttcgtt ttggcattta tcagaagccg
    13261 tatcacgtat ataatagttt ccgttttttc tgtcacaagc aaaacgttat cctccttttg
    13321 gaaatcaagg tatgatgaga aaaaaggggg attttatatg acaaaaaaag cagttttcgc
    13381 aggcggatgc ttttggtgta tggtcaaacc atttgaagag cagccaggga tcatttctgt
    13441 gacatccggt tatactggcg ggcatgtacc aaatccaact tatgaacaag taacaacagg
    13501 cacaacaggg catacagaag cagtagagat cgaatatgat ccagaagtta tttcttatga
    13561 tcagttggtg gaaatctatt ggcagcaaac cgatccgact gacgcattag gacagttcgc
    13621 tgatcgcggt gattcttatc gtccagtgat ctactataat gatgaagaag aacgaaatat
    13681 tgcagagcgt tcaaaacaag cattgcaaga cagcgggcga ttcgataagc caatcgtgac
    13741 aaaaatcgaa ccaagaagcg aattttatcc tgcagaagat taccatcagg attattacaa
    13801 gaaaaacaag ctccattaca atctttatcg agaaggttct ggacgtgcag gtttccttcg
    13861 tcgacattgg ggcgtcaaga aataagaagc cgaaaaagct acttatggaa caccgtatat
    13921 cagaggttgt aaaaccagca ttttgagcta atactgaggg tcagctgatt gaacaccatt
    13981 tagtggagag gttgtgaacc tgtcgcctag ctccaagtca caaggatttg gtaagagaac
    14041 actatatatc agaggttgtg acaagtcgtt tgtcacaacc ttttttcaat acctaagatg
    14101 aaggacaatc agcaacgcac tgctcaaaaa aggaatgaaa ggcagctttt ccaacttttt
    14161 tttatgacag ataaagtaaa cagaaaaaac ggatattcca aacagactag caagaaacaa
    14221 gatctgaagc aactcttcta gagacaagcc accactccaa gaaagcatca acagagtatc
    14281 gccagctccg agataggatt gaccatagcg aaggcacaca ccgcctacga taaacgttaa
    14341 cagcacactt tgccattgga agacgccggc ctgcaaccaa aaaatccata aaattgccca
    14401 agaacagaat agcgtgtgag aatggaccat gaagtaaaat aaatccatca acgaaaatag
    14461 caaagccgaa agcatccata tgaacgtcga aaaatttctt gcggaagaaa aagtaaagaa
    14521 ccaggcaaaa agaccaccac ataaggcttc tgccaacaaa tagtagaagg gcaaccgaca
    14581 cctgcaatag cggcagcgaa atcgttggac gatgatcgag agaatcggaa tcaattcata
    14641 ccaagaaagc gaacgacagc atttgacaca atgagatcta ggataaataa tagagtgacc
    14701 taacggaatc cgctgggcgg ttaaacataa aaatgatccg atacagcaac caatgataaa
    14761 ataaagtaac acgccatctc ccccttttca aaaccaagat acgcaagctt aaactaaatt
    14821 tttgtacata cataaaattt gaggtgaaaa aatgaacgtt tcaatcggat tagatatcgg
    14881 aaccacacaa accaaagcag ttgctttcac agatgaagcc caagaagcag catccgctta
    14941 ttttcgctac cctttgatcc aagaaacaca agggatggca gaacaagatc aggaattgat
    15001 ctttcaaggt gtctgcaccg ttataaaaga agtaattaaa cagttgcctt cagacacaat
    15061 cattcaattt gtctccttct cctctgcgat gcatggcctg atattgttgg atgaagagaa
    15121 aacaccgtta tctcgaatga tcacttgggc agataaccga gcagcggacg aagcagaaaa
    15181 actgaaatat accgtgcttg gcaaagaatt ttatcggcag actgggacgc cgattcatcc
    15241 aatgacacca ttgaacaaaa tcaagtggct gcaaaacagc caacctgaat tattcaaaaa
    15301 aactgcttat tttgtcggta tcaaagacta tatcttttat agattgtttg gcgaactgat
    15361 cagcgactac ggaacagctt caggaactgg atattttgat atccatgaat tccaatgggc
    15421 agaagaagtg cttgactact tgaacattcg aatagagcaa ttgccgcaag tctttccttc
    15481 cacctatcaa gtaacaggtc taccatcaaa aacagcagca gaactaggtt tgccaaatga
    15541 tatatcattt gttttgggag gggcagacgg gccgctttct aatctagggt taggtgcttt
    15601 tggtgatacg atcgccgctt tgaccgtagg aacgagcgga gcacttcgtt ttattactac
    15661 tcagcctcag ttccatccag aaatggagac gttttgttat gttctggatc agaaacattg
    15721 ggtagtggga ggagcgacta gcaatggtgc tggcatcttt gattgggcag ggcatacatt
    15781 tttacaggaa gtcgttcaat ctgctaaaga aaaaggagaa aatccatatg atgcactgct
    15841 ttcggctatc gcttttgtgc cagcgggtgc caatggtctg atttttcatc cctatttact
    15901 tggtgagcga gcaccgcttt gggacgcaga tgcgtttggc agttttatcg gtttacagcg
    15961 gcaacataca gaaaaagaaa tgatgaaggc gattctagaa gggatctgtc tcaaccttta
    16021 tcggatttta aaaggagttt gccaggaaaa tcagctcaca gagatccgtg ctacaggcgg
    16081 gtttgctcaa tctccgattt ttcgtcagat gatggctgat gtactgggtt acccgcttgt
    16141 tttccctact gtcacagaag catccgcatt aggagctgtg attttaggct ggagaagcat
    16201 cggaaagatc caaagtatca gtgaagcaca ggaattgata gaattgaaag agcaagttgg
    16261 agtacaagaa actgatcata aaaaatatca gaaaatctat ccattatttg tttcgacaca
    16321 aaagcattta gcacagactt atcagcaatt tgctcaactg cgagaagaat tagctgattc
    16381 tgaaaaatga ttggctttac ggataaaaaa gcgaactgaa aatcgttctc aggtaaatga
    16441 ttttgttgta cttttcattg aaagtctact attattttaa gtgtaagaaa atgttaggag
    16501 gcaagaaaga tgaaattcca acaaaccaaa gaagtattaa atcaattagt tgcagatcta
    16561 agccaaatgt ctgttgttgt tcaccaaaca cattggtaca tgagaggacc aggtttcctt
    16621 acattgcacc caatgatgga tgagttcatg gatgatttga acgctcagtt agatgaagta
    16681 tccgaacgct tgatcacatt agacggttca ccttattcta cattgcgtga atttgcagat
    16741 aacacaaaaa tccctgatga aattggccgc tgggatcgta ctattccaga acgtttggaa
    16801 acacttgttg caggctatcg ttatctagca gatctttacc aaaaaggaat cgaagtttct
    16861 ggagaagaag gcgacgatcc aacacaagat atctttatcg aattcaaaac agccacagaa
    16921 aaacgtatct ggatgatcca agctcatttg ggcaaagcgc cagagatcga cgcgtaaaaa
    16981 ctgttgttga acttgaccga aaaagaggtt gtgaaatcag cgttttgacc tgagtattag
    17041 agaaacaaac gtcaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    17101 cagctgattg aacaccgttt accaagaggt tgtaagcctg ccgttcagct ccgagtcaca
    17161 agaaacaatt ttaaaaaaca gcttcttatg ttttatggat attgtgactt gtgaccgagg
    17221 agttggcagg agaacaccgt ttattcaaaa taaggggttg tgacaagact tttgtcacag
    17281 cctctttttg tttttcattt tgacaggaaa cgaccctttt atataaaaga aagaaaagaa
    17341 gtaaagtttc atataaattt ttcaatcaag cagaaaaacg ttgacattga ctagattagc
    17401 gggtaatatg ttaaatggta ttgtttgccc cacaaagagc cccggaaact cgaagtgtat
    17461 gtcaaacatc agaacttaaa ttggaggaaa ataacgtgcg tacaacatat atggccaaac
    17521 caggcgaagt agaacgtaaa tggtatgtag tagacgcaac tgatgttcca ttgggacgtc
    17581 tttcaacagt tgttgcatct gtactacgtg gtaaaaataa accaactttc acacctcatt
    17641 tggatactgg cgatttcgtc atcgtcatca atgcagacaa agtgaaatta acaggtaaaa
    17701 aagcgactga caaaatttat taccgtcaca gcaactaccc aggaggattg aaatcaatca
    17761 ctgctggcga attgcgtgct aaaaattctc gtcgtttgat cgaaacttct gtaaaaggta
    17821 tgcttcctaa aaacacttta ggccgcaaac aatttacaaa attgaacgta tacggtggag
    17881 cagaacatcc acatgcagca caacaaccag aagttttaga catcacaaac ttaatctaat
    17941 aggagggaat ttcattggca caagttcaat atatcggcac aggccgtcgt aaaaacgcag
    18001 ttgctcgcgt acgtctagta ccaggaactg gcaaaattac tgtaaataaa aaagatgttg
    18061 aagaatacat tccacatgct gacttgcgtg aagtaatcaa tcaacctttc gctgttactg
    18121 aaacaaaagg cgcatatgac gtatttgtta acgtaaacgg cggaggatac gctggacaat
    18181 caggagctat ccgtcatggt atcgctcgtg cattgctaga agttgatcct gacttccgtt
    18241 tagctcttaa acgtgctggc ttacttactc gtgacgcacg tatggtcgaa cgtaaaaaac
    18301 caggtcttaa gaaagcacgt aaagcttcac aattctcaaa acgttaatat cctttatata
    18361 tcaacgtttc tggcactttt caattatttg gaaagtgctt cttttgtttt taagaacaca
    18421 tttaagaaca catatcaatc acacttattc aatcctctct gaaaagcttc aaaaacagcc
    18481 ttatcaacta tatctggtgt gttcacatat atctttgttg tagaaatatc tgagtgtgtt
    18541 aaagcatttg aaatttgatt catatctgct ccaccttgtc tagctatagt tgcataagta
    18601 tgacggagtt tgtgtggaga taaatgaact aagtcaggat gccttctttt aattgaattg
    18661 attcgatagt ttaggtaatc ggcatgaaca ggacaattta cttctccaga tggtttagta
    18721 taagtaaaga gatactgatc taacgtttgc ttaattttta atttcaacaa ttgatcagct
    18781 tgtaccgatt tccattcact tagcaatgtc attacaactt ctggtacttg aatgacggta
    18841 tcttttcgac cttttgtaaa cttttttctt tgatatttat ctaaagtatg tttaagacgg
    18901 accgtttgtt tttcaaaatc aatatatttc cattgcaaag cgtaagtctc actttttcta
    18961 tcaccaagat acagggtaat cataaaaagt gtgtaatcat gaaaagtaag taaatgatta
    19021 ttaaaatcat catttactgc ttctatccaa ttagtaagtt cttttgatga tagtgcttgc
    19081 ttaccttctt gtattctttt ttcttctaaa gcattttttt taggtgcagt gatactttga
    19141 ataattttag tagttcgatt aaattcgata taatttagta tttctgcaat atcgaacatg
    19201 gaacgaacat atccttttat gatttttata tttgcatatt ctttcgattt tttggttaat
    19261 tcatctgata ttatctcagt attgatattt agataattca ttgcgtattc accaaacata
    19321 ggtaaaatat gcaaccgaaa tatatctttt gtattactaa tggttatatc actaggaggc
    19381 ctgtttgatc taattgtttg acctaattca tatctaggaa gccatttaga ttcgtaaaat
    19441 tttttaaacg ttatttttcc ttttaattct aatgagtttg cattctcctc tctcaaaact
    19501 ttttctattt tcttcttcat atcattttcg gctttaatcg ccaattgcct atttggaaat
    19561 gttttttgat acttttctga tgcaattcct gttatttctt taaacctttt tggatatctc
    19621 agattagttt tatatttccc atttgaaagc tttgtaggca aaatcctcac tccttaaaaa
    19681 aagtaaggca gtatatttca ctaattttag tatatgcggc gagaagaaaa tttcaagtta
    19741 ttttaaatgt attcataaag tctttaatat cttctacgtc aaagcgaact acaccaccaa
    19801 ctcttattac tgggaaatta tatatacgaa tccacttatc tagagtatta ttatcgattc
    19861 ctagaaatgc acaggtttgt tttttattca ggtagactct ggattctata aaatcatctc
    19921 ctaaaatagt agttatgaaa tttttgaatt gctttgggta agcacatagg aatttcacct
    19981 ctccccagtt tacggcgagc cttacggaag tatcattatt tgctgctatt gtcatggcat
    20041 tttgttttcc cagaacaaaa agaaatctct aagtatttgt cgctaatcag gtcatggcgt
    20101 acttgagttt ctagctcgaa tagcagggat cgttattcaa aaattgtatt taattgtcaa
    20161 tgatcaaaaa tagttttttt cctattcact attaataacg ataaacatgt ataaaaagac
    20221 acactacgaa aaaagttaaa tggagtttac tga
//
LOCUS       DAYYME010000044        19629 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000044 DAYYME010000000
VERSION     DAYYME010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.044"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACHBFW_001309"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACHBFW_001309"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFM9317622.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACHBFW_001310"
     CDS             complement(1172..1867)
                     /locus_tag="ACHBFW_001310"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFM9317623.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACHBFW_001311"
     CDS             complement(1880..2596)
                     /locus_tag="ACHBFW_001311"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFM9317624.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="ACHBFW_001312"
     CDS             2926..3534
                     /locus_tag="ACHBFW_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFM9317625.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="ACHBFW_001313"
     CDS             3859..4182
                     /locus_tag="ACHBFW_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM9317626.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="ACHBFW_001314"
     CDS             4364..5734
                     /locus_tag="ACHBFW_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFM9317627.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="ACHBFW_001315"
     CDS             complement(5864..7165)
                     /locus_tag="ACHBFW_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM9317628.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="ACHBFW_001316"
     CDS             complement(7519..9510)
                     /locus_tag="ACHBFW_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFM9317629.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="ACHBFW_001317"
     CDS             complement(9654..9920)
                     /locus_tag="ACHBFW_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9317630.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACHBFW_001318"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACHBFW_001318"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFM9317631.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACHBFW_001319"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACHBFW_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFM9317632.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /gene="def"
                     /locus_tag="ACHBFW_001320"
     CDS             13264..13827
                     /gene="def"
                     /locus_tag="ACHBFW_001320"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM9317633.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="ACHBFW_001321"
     CDS             complement(14080..14601)
                     /locus_tag="ACHBFW_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFM9317634.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="ACHBFW_001322"
     CDS             complement(14604..15446)
                     /locus_tag="ACHBFW_001322"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFM9317635.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="ACHBFW_001323"
     CDS             complement(15439..16164)
                     /locus_tag="ACHBFW_001323"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFM9317636.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="ACHBFW_001324"
     CDS             16340..16636
                     /locus_tag="ACHBFW_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317637.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16892..17066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17204..18796
                     /locus_tag="ACHBFW_001325"
     CDS             17204..18796
                     /locus_tag="ACHBFW_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM9317638.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="ACHBFW_001326"
     CDS             18809..19549
                     /locus_tag="ACHBFW_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9317639.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5617 a         4028 c         3724 g         6260 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagccattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaagtaaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgagatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttc
//
LOCUS       DAYYME010000045        19141 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000045 DAYYME010000000
VERSION     DAYYME010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.045"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(269..790)
                     /locus_tag="ACHBFW_001327"
     CDS             complement(269..790)
                     /locus_tag="ACHBFW_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFM9317640.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            988..4668
                     /gene="nifJ"
                     /locus_tag="ACHBFW_001328"
     CDS             988..4668
                     /gene="nifJ"
                     /locus_tag="ACHBFW_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFM9317641.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            4857..5744
                     /gene="cdaA"
                     /locus_tag="ACHBFW_001329"
     CDS             4857..5744
                     /gene="cdaA"
                     /locus_tag="ACHBFW_001329"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFM9317642.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5741..6910
                     /locus_tag="ACHBFW_001330"
     CDS             5741..6910
                     /locus_tag="ACHBFW_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFM9317643.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6934..8289
                     /gene="glmM"
                     /locus_tag="ACHBFW_001331"
     CDS             6934..8289
                     /gene="glmM"
                     /locus_tag="ACHBFW_001331"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFM9317644.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICARYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8361..10166)
                     /gene="glmS"
                     /locus_tag="ACHBFW_001332"
     CDS             complement(8361..10166)
                     /gene="glmS"
                     /locus_tag="ACHBFW_001332"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFM9317645.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10576..10866
                     /locus_tag="ACHBFW_001333"
     CDS             10576..10866
                     /locus_tag="ACHBFW_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317646.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10879..11904
                     /gene="sppA"
                     /locus_tag="ACHBFW_001334"
     CDS             10879..11904
                     /gene="sppA"
                     /locus_tag="ACHBFW_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM9317647.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11907..12566
                     /locus_tag="ACHBFW_001335"
     CDS             11907..12566
                     /locus_tag="ACHBFW_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM9317648.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12723..13655
                     /locus_tag="ACHBFW_001336"
     CDS             12723..13655
                     /locus_tag="ACHBFW_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317649.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            13809..15098
                     /gene="tig"
                     /locus_tag="ACHBFW_001337"
     CDS             13809..15098
                     /gene="tig"
                     /locus_tag="ACHBFW_001337"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFM9317650.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            15345..16595
                     /gene="clpX"
                     /locus_tag="ACHBFW_001338"
     CDS             15345..16595
                     /gene="clpX"
                     /locus_tag="ACHBFW_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFM9317651.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            16678..17265
                     /gene="yihA"
                     /locus_tag="ACHBFW_001339"
     CDS             16678..17265
                     /gene="yihA"
                     /locus_tag="ACHBFW_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFM9317652.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(17339..17494)
                     /locus_tag="ACHBFW_001340"
     CDS             complement(17339..17494)
                     /locus_tag="ACHBFW_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFM9317653.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            17630..18958
                     /locus_tag="ACHBFW_001341"
     CDS             17630..18958
                     /locus_tag="ACHBFW_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317654.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT         6257 a         3383 c         4134 g         5367 t
ORIGIN      
        1 gtatgaaaat ttaagaatta gttagtatgc gcaagtcaaa aatatgaata aattatttgg
       61 tggagataat ttgctcagct tgtcataaga aatgagataa tctggcagat gcaagctttt
      121 ggacaccatt tattcggaat aaggaggctg ggacagaagc gtttaactcc aagaaataag
      181 aaggagttgc ttctgcttcc gccgtttata ggtgtttaga gcgtgagaca aaagtgttct
      241 ttacttttgt cctacgctcc ttattttttt attttgtttg gatcaattct tttaattcct
      301 ctattgaaat agattcatca aagatttctt tacccaagat gacagtcgga acaaatcgaa
      361 tatgtgcttg ctcagcttcg ttgatgacag cttggctcag ctgttcgtct ttttgttctg
      421 tcagctttag tttatctact gcaaaatccg ctacttcttg gagagaaaga tgtttccact
      481 cgtcttgagt atcgaatatt tgtttgatct ctttgatcgc tttttgacca tcgctgattg
      541 tgagataccg gtgcatcacg tttcctcgct gcaagctttc tttttcttta tcgaacaatt
      601 tgatcactcg ctgcaattgc ccgctttgga ctgcttcttc taatagctca taagattctt
      661 caaaccattg tcggcaatac ggacaagata ggttgatgaa ttcgaccatt tttttaggag
      721 cattcgatga accataaagg attccttttt gtgtatttgt tttcgttgca tcaatcactg
      781 atatatccat gttaaaacac ctctttcgct tatagtttat acatgtctga caaaaaaagg
      841 caacgattta gactgtttgt ctaaaaatta gatgaccaat tcaaatagac atatgtgaaa
      901 tatttttata aataaaagaa aaaatggaat gttttacgtt ataatggaac agataataag
      961 agagaatatg aacaaggatg atgaatcatg cgaaaaatga aaacaatgga tggcaatacg
     1021 gcagccgcat atatttctta tgcatttacc gagattgctg caatatatcc aataacaccc
     1081 agttcgacca tggctgaatt agtagatcaa tggtcagcag caggaaaaga aaatgtattc
     1141 ggtcaaccag taaaagtagt agaaatgcaa tccgaagcag gagcagctgg tgtggtccat
     1201 ggatcattga aaactggtgc actgacaact acatataccg cttctcaagg gctgcttttg
     1261 atgataccga atatgtacaa gattgctggg gaactactgc caagtgtttt ccatgttgct
     1321 agtcgggcag tgacgacaaa tgcgttgaat attttcggag atcacggaga cgtgatggct
     1381 gcaagacaaa caggatttgc tatgttagct gaaagcagtg tacaagaagt gatggattta
     1441 gcacctgttg cccatcttgc ttctattgaa ggagaagtac cttttttgaa tttctttgat
     1501 ggattccgta cgagtcacga gattcaaaaa atcgaagttt tggaatatga agaattagct
     1561 cctctgatca atcaggagaa attagcttca tttcgtaaac gagggatgaa tcccaatcat
     1621 ccgactgtta gtggtacgaa ccagaatcca gacatccatt ttcaacaacg ggaaacgatc
     1681 aacccctatt atgataaatt acctgaaatc gttcaaaaat atatgaaaga gatcaaccga
     1741 ttacgaggaa cttcttatga cttggtgact tattacggag cagacgatgc ggaagaagtc
     1801 attgtttcta tgggatcagt tgcacagacg attgaacaaa cgattgatta cttgaatgca
     1861 aacggacgga aaacaggttt tttgaatatc catctttatc gtccttttcc agtggaaaca
     1921 ttcgtgcaaa agttgcctca tacagtaaaa acaattgctg ttcttgatcg aagtaaagaa
     1981 ccaggggcag gtggagaacc tctcttatta gacgttcaaa gtgcgttata tgattctgaa
     2041 cttcgcccgg ctgttattgg tggtcgctac ggattggggt ctaaagatgt gactcccgac
     2101 caaatctcag cagtttttga tgaattgaaa aaagacccaa gcatacgcaa aaaacggttt
     2161 accataggga tcgtggatga tgtgacttat caatctttgg aaccaaagga aagtctggat
     2221 ttgacagaac cgcaaacatt ccaggccaag ttttggggat ttggatctga cggaacagtt
     2281 ggagcaaata aatcagctat caagattatt ggagatcaca ccgataaata tgcgcaaggc
     2341 tatttttatt atgattcgaa aaaatctggc ggtctgactg tctctcatct tcgttttgga
     2401 gatacgccga tacgttcagc gtatcttgtc gagcatgcgg accttgttgc ctgtcacaca
     2461 ccagcttacc tgcatagcta tgacttggtc aaaggattga agccaggtgg cattttttta
     2521 ttgaatactc tttggagcga cgaacaatta gaaacgcatc tgcccttaaa attaaaacgt
     2581 tacttggctg aaaataacat tagattctat acgatcaatg ccatgagatt ggcccaagaa
     2641 gtcggtttgg gacgccggat caatacggca atggaaacgg ctttttttaa attagctgac
     2701 atcattcctt ttgatgaggt gcttccgctg ctaaaagaag aagccctgaa aagctatgga
     2761 cataaatcaa tggctatcgt agaaaaaaac atacaagcaa tcgatcggac gaccgaactg
     2821 cttcatcaag tcgcgatccc agaccattgg agaacgttgg agatgcctga aaaaatcaat
     2881 aaaaggaaca ctggttatat acaagaaata cttgaaccag tgaatgcaca ggaaggaaat
     2941 caactctctg ttggtacact tgtagcaaat ggaatgacaa ctggcgaaat gcctttagga
     3001 acggcttcta tggaaaagcg gggcgtcgct ttagaagtac ctgagtggat tagtgatcgc
     3061 tgtacgatgt gtaatgaatg tgcctttgtt tgtcctcatg ctgccatccg tccattttta
     3121 gcagatgaag aggaaatgga agaagcgcca gaaggattta tcgtgagaga aatgcgaggc
     3181 gcagacggcg taaaataccg tatccaagtt tcagtggaag actgtacagg ttgtggtctg
     3241 tgtgtagagg cttgtcccgc aaaagaaaaa gctttagtga tgaagcctta tgaagaacag
     3301 aaagaacaag ctgtgaattg ggcttttgca atgacattaa gacaaaaagc aaatccagtc
     3361 aaaggaaaga atactgtctt gtctacacaa ttcaatcaac cgcttcttga attctcgggc
     3421 gcttgctcag gctgtggcga aacaccttat gtaaaattat tgacacaaat gttcggtgat
     3481 cgaatgatga tcgcgaatgc gacaggctgt tcttctattt ggggcggtgc ttctcctgct
     3541 tctccttata cgaccaatga atgtggtcaa gggcctgcat ggtcaaattc tttattggaa
     3601 gataatgctg aatttggtta tggtatgctt ctagcggcac agactcgtcg cgaggctttg
     3661 gcaagttcaa tcaaagaagt attgccaaaa gtatcagaag aactacgaga attgatggaa
     3721 gactggttgg tacacatgtt tgagagcgaa ggaacgcagc aaagagcagc aaaactgcaa
     3781 gctgcgatcg aaagcgaacg atcaattgtc cctgaactgg aggatatata caagagaaag
     3841 gatctattcg taaaaaacag ccaatggatg attggtggag acggatgggc ttacgacatt
     3901 ggttttggcg ggatcgatca tgtacttgcc agcggagcag acgtgaatat tcttgttttg
     3961 gataacgaag tctattccaa tactggcggt caaacctcaa aagcaacacc agcttcggca
     4021 atcgctaaat tttcggctag tggaaagtat gcttcaaaga aagatctagg aatgatggcg
     4081 atgacatatg gaaatgttta tgtggcgcaa attgcttcag gtgccaatca aatgcagacg
     4141 ataaaggctt ttgaagaagc agaaaagtat cctggacctt ctttaatcat tgcctatacg
     4201 ccttgtatca cacatggttt gcttggtgga atgaaagaat cgcttaaaga agcaaaagaa
     4261 gctgtttctt ctggctattg gtcattatat cgatataacc ctgcaatggt cgaaaaaaac
     4321 aagaacccaa tgactttgga ctacaaaaaa cctgatttct ctgctatgcc tgatttcatg
     4381 aggaaacaag ttcgcttttc ctcgctagag aacgcaaatc caaatcttgc aaaagcttta
     4441 tttgaaaaga ctatccaaga cgctaaaaca cgtttttaca actatgctgc actgacggga
     4501 gaattagaaa aaatcaagaa acgacttgaa ccagaggaat ctatcgatgt acccgttaag
     4561 actagaaaag aaaaaacgac ggaccttgaa gcagaagaaa gaagagctgc cagagcagaa
     4621 cgacgtaaaa aaatggaaca agctacaaaa gaaaaaaagg aaaaataaca gaaaaaatat
     4681 aatgaataat tctctgaagt ttaaaaatat ttattgcaga gaaaaataaa acgcatactt
     4741 gcaattttcc tgaaaattat gcgtttttct caagaaaaat acgagcagat cagcctctaa
     4801 aattaaaaca tatgatatac tagagaggaa atctcaaaag agaagagagg ctttttatgt
     4861 catttcaaat cggggaactt tttaatttgg actattggca gcaaatgttt tccggtgact
     4921 tgttctctct gaattttttg gtgaacatac ttgatattct ggttgtctgg tatcttgtat
     4981 acaaactgat ccaactgctg cgaggcacaa aagcgattca gcttctgaaa ggtgtagcta
     5041 tttttattgt tatccgaatc gtagcggaaa tcattggtct gcacaccttg tcttggctga
     5101 tgaaccaagt cattacgtac ggggtgatag ctgctatcgt gatttttcag ccggaaatcc
     5161 ggagaggact ggaacatctt ggacgcagtt cgcttttcaa acaatcaaaa agcgaacagc
     5221 aggaagatga gaagatgatc ttgtcttttg acaaagccat ccaatatatg tccaagcgaa
     5281 aaatcggtgc gctaattact atcgaacggc atactggatt agatgagtat attgagacag
     5341 gaattgcatt agatgcagat atcactggag agctgctgat caatatcttt attcctaata
     5401 caccgctgca tgacggtgca gttattgtaa aagaaggaaa gatcgcagta gcaagcgcct
     5461 atcttcctct atcagaaagt atgttgattc caaaagaatt cgggactcga caccgggcag
     5521 ctgtcggtat aagcgaagtc agcgacgcga ttaccatcgt tgtctctgaa gaaactgggg
     5581 atgtcagcat tacattagat aatgaactga tggctggctt atcccagcaa gaatatttgg
     5641 caattttgcg tagagaactg attgctgaag agaaaacaaa aaacaagaaa aacgtgattc
     5701 aatctttcct agacggtatg actaagggag gggatcgcaa atgatctcaa aagagaaacg
     5761 gacaagcatt ctttacagtt ttattgcatt gctgttcagt attgcgctgt acttcaatgc
     5821 aaatggccag tccgttccaa atacgctttc tggaaatgaa gcgtataatc aaacagttac
     5881 tggggtgcca atcaagattt cttatgattc aaagcgctac tatatccatg gatatgaaaa
     5941 tgcagtgact gtcaaacttt ccagtgcaaa ccgagttcag ttgaatgcag aggcaaacga
     6001 agatacacgg atgttccgcg tcacagcaga tatcactaag ctgggcgaag gaacacatga
     6061 ggttccgcta aaaattcaaa atttgagcag tgctgtcaca gcaaagatcg aaccatcgac
     6121 tattacagta acggttgaga aaaaggtaat aaaagatttc gatgtggaaa cgttgattcc
     6181 ttcaacgttc acaccatctg gatatgaact tgatgatact tccgttagtc ctaaaaaagt
     6241 ctctatcact acaggtgaca aaacattggc agaaatcaaa cgagtagtag ctgatgtgga
     6301 cgcttcgatg gtcacagatg atgggattaa tagtgaggtc ccaatacagg cgctgaatgc
     6361 agcaggggaa gtattaagta tcgtctctga tccagtgcaa gtaacagtga gagctgattc
     6421 gatcaagcca agcaagtcgg tacgacttta tgggatacag caaggaacac cagctgccgg
     6481 ggtagaaagc tatgatttca gtttttcaaa actggaagca gaagtaagcg gctctagcga
     6541 tttgcttgca tcgatcggtg acagtattgc tgtaccgatc aatgtctcag gtatcaatca
     6601 tcgaacaact cgtaaaattg aaataccagt agaagagggg ttaagtatca agcccgaatc
     6661 agttagtgtg gagattacac ccgtgttgca aacgtcaagc agtagctctg cttcgacgag
     6721 cagtcggaca caagaaaata caagtacgag tagaagtatt tcatctgctg attcaggaac
     6781 ggaaccatcg acaacaacca tttcttccag ttcggaatct gggacaagca gctcgaccac
     6841 aagtacagaa gaaacatcag aaacaagcga atcaacacaa atgagcagaa caaattcctc
     6901 agaaaattaa gttaaaggag taattttaca actatgggta aatattttgg aacagacggc
     6961 gtacgcggtg aagccaacaa ggaacttacg ccagaacttg catttaagct gggacggtgc
     7021 ggaggttatg tcctaagcca gcatgaagaa gaaggaaaac gcccacgcgt gttagtaggg
     7081 cgggatacaa gaatctcagg tcaaatgtta gaagaagcgc tgatttcagg acttttatcg
     7141 gtgggtatcg aagtgttcca gctaggtgtg atctctacac caggtgttgc ttatctgaca
     7201 cgtttgcaaa aagcaagcgc aggggtcatg atttctgctt ctcataatcc agcacaagat
     7261 aatggaatca aatttttcgg tgcagatggt tttaaactag tagatgacca agagctagaa
     7321 attgaagcat tattagatgc tgaagtggat gaattgcctc gtccatcagc tgaaggattg
     7381 ggaactgtag aagaatttcc agaaggctta ttgaaatatt ctcaattcct tgtacaaacg
     7441 atcaacggtg acctttccgg tttgaccatt tgtgtcgatg cagcaaacgg agcaacttca
     7501 acaagtgtta atcgtttgtt tgccgatctt gaaacagatt tttataccat gggcacaaat
     7561 ccagacgggt taaatatcaa tgatggcgta ggctctaccc atccagaacg tttagctgag
     7621 atggtcgttg aaaaaggagc agatgctgga ctagctttcg atggcgatgg cgatcgtatt
     7681 attgcggtgg atgaacttgg aaacattgtc gacggtgata aaatcatgtt tatctgtgct
     7741 agatacttgg cagaaaaaaa tcgattgaaa aaagatacga tcgttacgac tgtcatgagt
     7801 aacttaggat tccacaaagc ggtggaagga atcggtttga aagatgtcgt tacacaagtt
     7861 ggtgatcgtt acgttgtaga agaaatgcgc aaaaacgatt ataactttgg cggcgagcag
     7921 tctgggcaca tgattttctt agattttaac acaactggag atgggatgct ttcaggaatt
     7981 cagttactga atatcatgaa acagacaggt aagaaattat cagagcttgc agcagaggta
     8041 acaatctatc cgcaaaaatt agtgaatatc cgagtaagtg ataaaaacgg tgcgatggat
     8101 gttccggcaa tcaaaaaagt gattgaagag gcagaagctg aaatgggatc agaaggaaga
     8161 atcctagttc gtccttccgg tacagaacca ttacttcgag tgatggcaga agcgcctact
     8221 gatgagaaag ttaattacta tgtcgataaa atcgcggcag tagtcaaaga agaaatcgga
     8281 ctggattaag agatattttc ataaaaaata aaagaacaag gaccagtgtc agatgtctga
     8341 cactggtcct tgttctttta ttattctact gtcactgatt tcgctaaatt acgtggtttg
     8401 tcaacgtcga atccccgtaa tagcgttgcg taatatgcaa gtaattgagt cggtacgact
     8461 gtggttagag tagttagata agggtggaca gttggtagca ccagctggtc actttccttt
     8521 tctaatccct ctgagacaat aactagtgtt ttagcgccac ggctttcgac ttctttcagg
     8581 tttcctcgag tatgcgcacc agtcacttct tcactgatga ttccaatcac tggtgttcct
     8641 tcttcgatca aagcaatcgt tccatgtttc aattcgccag cggcaaaacc ttctgcctgg
     8701 atataactga tttctttcaa tttcaaagca gcttcaagag aaacgttgta gtcagcgcca
     8761 cgtccaatgt aaaaggcatt tcttgtatcg ctgagatata cttctgccaa ctcttctaaa
     8821 taatcttttt gggcgatgac cgattccatc gcattagcaa ctaaactcaa ttcatgagtg
     8881 atatcgaata caagagaagt ttcaaattct tttttatcac cgatagcttt ggctaataaa
     8941 gttagtacag ctatttgtgc agtataagct ttcgttgatg ctactgcgat ttcgggtcca
     9001 gcatgcaata gcagtgtaaa ggatgcttcc cgcgaaagag tagaaccagc aacatttgta
     9061 atcgtaagtg aagggtagcc gagacggttg atttttacca gaacttggcg gctatctgct
     9121 gtttcgccac tttgactcaa gaaaataaag aatggttttt ttgaaagaag aggtgtgtta
     9181 tagccaaatt cactggctaa atgaacctca acagggatct gtgtcaattt ttccagagtc
     9241 tgtttacctg ctaaccccgc atggtaacta gttccgcaag caacgatata gattcgatcg
     9301 ctatcagaca atgtatcgac cagcttttga tcaagagaga cacgattgtc ttctgttacg
     9361 tatttttgag cgatacgacg catgataata ggctgttcgt cgatttcttt cagcatataa
     9421 aaaggatagg tacctttctc gatatctgaa gaatctactt gtgcttcgaa tgaagaacgt
     9481 tctacgatgt cacctgcaaa attttgaata ataacttttt ctgatgtgac ggtaaccatc
     9541 tcttcatctt caatctcaac gaattccttc gtatgagcaa gcatagccat tgcatcgctg
     9601 gcaatcacat tgaatccatc gccgagtccg attaatagtg gacttttatt tttagcgacg
     9661 taaataacat caggtgctgt ccgatcaatc aaagcgaaag cgtaagaacc tttgatgacc
     9721 cgcaaagctt ttaaaaacgc ctcttgcgtt gtagtatcct tcgcgaaagt ttcaattaga
     9781 tgagcgataa tctcagtatc ggtctcacca ataaagtggt catttgctaa ataagcaatt
     9841 ttaagttctt caaagttttc gattacgcca ttgtgaacga gcacgaagcg tccactttgg
     9901 gaagtatgtg gatgggcgtt ttcttcagaa ggttgcccgt gagttgccca gcgtgtatga
     9961 ccaataccta tatttcctgt agtatcaata tttattttgt ttttcaaatt ttgaattctg
    10021 ccttgtgctt tgaccagata atcagttgtg ccatctgaga caaagatacc agctgaatca
    10081 taaccacggt attctaattt ttctagaccg tcaatcaatc ctggtgttac attttttaat
    10141 ccaatcattc caacaattcc gcacataaaa aacatcctac tttctataat gaattcgtat
    10201 gaatgcttcc agctgcagga aaatcctttt gttttatctt tgcagatatt ttttgtggat
    10261 tttctgtaga gtgcagtccc tagggccatc cgccgaatct ttcgataaac cctctcctcg
    10321 tcatctgttt acacagacct ggcgctatcc taaaaggtat aactatcctc ctcttttgtt
    10381 ttttatctgg aaacatttaa aatcataacc ttttgcgaat gttcttgtca acaaaaaaag
    10441 aataaaaaat aaattggtat atatcaaatg ctttgtcaaa tgctcttttt atgtcaaaaa
    10501 ataatgggtt tttatctatc atctttttca ggagttcggt acaatagaaa gtaatcggat
    10561 agaggaggag taaaaatgga ttcaaaaatt gagatcatga cactgggaat gttaaaaaaa
    10621 caattgtcca aattcgaagc atcagctggt gtatcagatg atacgaaaat atttttagat
    10681 actggctggg atagcatcca agaaatcgca cctgatgcct tggaagttgc tgaagctcgt
    10741 gagtttacgg tagaagatga attgacaaaa gagtcgtttt ccgggtatgc gcgggaagaa
    10801 aaagcagagc ggttcgacac ttctgagcag tcagaaactg tgatcgtcat aaaaaacttg
    10861 tattagaaag gagaaagaat gaataagaga cgttgggcag cagttctgat tgctgcagga
    10921 ttatttgtgg tttcgttagt ttcagctagt ttgactaagc ctcaggaaaa cgatcaaatt
    10981 gagggattga acagttggtt atatggggat gaagaactag cttcaaatgt gttggaagag
    11041 ggagacagta cgcaaaaaat cgtaaaatta tcagtagatg gaacgattgc tgcgggaggg
    11101 tcttcaggtt tatttgcttc tgaaggttac aatcataaaa attttatgga acaactgaag
    11161 gcgattgaag aagatccaac agtcaaaggt atctttttag aagtaaattc tcctggtggc
    11221 ggtgtctacg aaagtgcgga aatcgctaag aaattggata cgattcgtaa agaacatgat
    11281 atcccaatgt atgtcagcat gaaaaacatg gcagctagcg gtggctacta tatttctgct
    11341 caagcagata aaatctttgc aacagaagaa acagtgaccg gctcaattgg tgtgatcatg
    11401 tcaggattga attattcagg acttctagaa aaactgggtg tggaagatac aacagttaaa
    11461 agcggagcgt tgaaagatat gggttcagga acgcgaccag agacaaaaga agaagaagcg
    11521 gtgcttcaag catatatcga cagcgcatat agtcgttttg tagcaatcgt cagcgaggga
    11581 cggaataaat ctgaagaaga agtcaaaaaa attgctgatg gaaggattta tgatggtgtc
    11641 caagcaaaag aagtaggttt agtcgatgaa ttgggatttc ctgaagatgc tttaaaagct
    11701 atgcgaaagg aacaacagct tgaagatgcg gaactagtag aatattcttc cacgccatct
    11761 gggtttgcta gtacatggtt aggaagtaaa ctagcagaat tccagggatt gaagccttct
    11821 gagactagtc agatcttatc cgttcttgag aatctaggga cagcggaatc accaaaagca
    11881 atgtattatt acggaggtga gtaagcatgt cagagctttc aaatgaaaat aaggaacaag
    11941 aaaaagataa gcaacagcag cctgctgtag gacctgactc caacttttcc caaaaggtac
    12001 tgagctcttt tgaaagagag cctttgacgc ccgaagaaat caaaaagaaa caacgtaaat
    12061 ggaaaaagta tacggacaca agaaatgaac cgccaaaagc aaatgatttt cctaagtatt
    12121 tctatgcagg tttttttatc cgaatgtttg ctttcttggt cgatcttttg tgtatcggag
    12181 ctattacgcg tttaacactt ggtattgcca aaaatctagg ttggcttaat ttttcagatt
    12241 cttatctgag tatctacgga ttcacagcct tacttattta tctagcgtat tttattttat
    12301 tgacgaaatt caatcatggt caaacgattg ggaaaatgat ctttggtatt cgagtcatca
    12361 gctttgatga agaagaactt agctggtcaa ccgttttagt tagagaaggt gcttgccggt
    12421 tcattctaaa atttcctttg ctgatgttag ggtatcttcc agcaatcttt aatcagaaaa
    12481 agcaacatct aggcgatttt ttcactaata caagtgttgt tacgatcaat ctgatcaaag
    12541 cattcaacca ggaagtcaac agctgatgag caataaaaag cagtaacagt tgaaagcacc
    12601 ttctaaaagt cagatctaaa atctagcttt tagagggtgc ttcagttatt tttaattgtc
    12661 atgctttttt tgttttgcta ctattgaaca tgagctgaac atgagcaaga ggaggacact
    12721 gcgtgaagcc gattgatttt ccagaaaaat atgagaattt aatgcgagta gcacaacaag
    12781 cactagcaaa ccagcaatat cagcaggcaa aagaattatt tcaacgagct tacgagttga
    12841 aagaaagctt tgaggctaat agcctgcttg ttttttgtct ttacgagttg gacgagaaaa
    12901 aagaagcatt gaagcaagca ctcttgcatg aaaaacagta tcttcaaaat gaggagtttg
    12961 ctgaatttta ttttgacttg ttaatcagta cacaagattt tttatatgct agaaaattga
    13021 tagcaagtac agatttttat gaagctttcg agcagcgggt cattgaaaaa atccaatttg
    13081 cagaagaatt atccggacaa atggagaggc agaagatcaa ggcactccac aataaatcgg
    13141 aggaacttcc ttcgttggaa cctgcacgtc agcttgcgtc tattgatgag atcgaacagc
    13201 ttccttatca tgagtttatc caaacagcga gtaaacttat tgtatcgcca gaggtccata
    13261 ttttagcaag ggcaaaatta ctggagacac ttaggcaatt gaacgaatgc catcctgttt
    13321 tctatttgac gattgaagaa aagttagtca aagtcatacc aaaagatctg ccaaagccgc
    13381 agcagcaggc aagttaccag caattatgtg tattttcaga tcgttacggt aatgaagatg
    13441 cgctgcttag ttccgtgtta aatgaagaat tcacgttgca atctgcaatt gtttatcctg
    13501 tctatgatac atacatcgaa gacccgaagc gctggtttca actgactgta gaggcgtgta
    13561 caggaaaaac gatgtatgaa ggtacagaag atgaaaaaca agatttttta aaaaaaagag
    13621 aaaaaatttt gcagcaaatg atcttcttcc attagatttt atcagtgaaa aagataaaaa
    13681 tgttatgaaa aacaaggatt ctatccattc aatatgttta caatttgaaa gggttagtgt
    13741 actatttaaa ggtatgcatg aaacgttttt gatgtataaa atgaatttct aaactcggag
    13801 ggaattaaat gactgcaaca tttgaaaaaa aaggcacgaa tgatggtgtt ttaaaattta
    13861 cgattgcgca agacgaaatc caaaaaggat taacaactgc gtttaataaa gtgaaaaaaa
    13921 gcttgaacgt acctggattc cgtaaaggga aagtttctcg tcaagtattc aaccgtatgt
    13981 acggtgaaga agctttatat gaagatgcat tgaatgctgt attaccagaa gcttatgaag
    14041 ctgctgtcaa agaagctggc atcgatcctg tttctcaacc aaaaatcgat gttgaaagca
    14101 tggaaaaagg tcaagactgg gtaatcactg ctgaagtaac agtgaaacca gaagtaaaat
    14161 taggacaata taaagatcta gttgttgaaa aacaagaccg tgaagtaaca gatgctgacg
    14221 tggatgcacg tatcaaacgt gaacaagaag cacaagctga attagttatc aaagaagatg
    14281 ctgcagctga aaatggcgat acagtagtaa tcgacttcga aggctttgta gatggcgaag
    14341 cgtttgatgg cggaaaaggc gaaaattact cacttgaatt aggttcaggc tcattcattc
    14401 caggttttga agatcaatta gttggtcata aatcaggtga aagtctagat gtaaatgtaa
    14461 ctttcccaga agattatcaa gcagaagatc ttgctggaaa agaagcagtc ttcaaagtga
    14521 caatccatga agtaaaagca aaagaagtgc ctgaattaga tgacgaattc gcaaaagatg
    14581 ttgatgacga agtatcaaca ttagacgaat tgaaagaaaa atatcgcaaa gaattagtag
    14641 aaacaaaaga aaaagcagcg gacgatgcaa aagatgaagc agcgatccgt atggctgttg
    14701 aaaacgcaga aatcgttgaa ttgccacacg tgatggttca tgatgaagta catcgttcaa
    14761 tggacgaatt cttgaacaac atgcaacgtc aaggtatctc tcctgaaatg tactatcaat
    14821 taactggttc tacagaagaa gacttgcata aacaattcga aggtgaagca gaaatgcgca
    14881 caagaacaaa tcttgtgatc gaagcaattg cagctgctga aaatcttcaa gcaacagaag
    14941 aagaaatcaa tgcagaaatc caagaattat cagaaacata caacatgcca atcgaacaaa
    15001 tcaaacgcgt gttgacagaa gatatgctgg aacatgatgt gacaatgaaa aaagcaatcg
    15061 aattgatcac aggtacagcg acagaagcga aagaatagaa atcaagtaat aaatcatttc
    15121 tgaagaagct acttctagaa aattgtttat tcgaaataag ggactgtgac acgattttgt
    15181 cacagttcct tattttttgt taagagtaaa aaatgcaaag gtataggtca gttgatcctt
    15241 tgttaaacac tttttctttt tactaaacta tgttatgata gtaaaagact gtaaagtctt
    15301 tgatagtttc ttgcatactt tttgcaagtt aaggggtgaa gaccatgtat gacaatccaa
    15361 gtagcaatga gaccgtacgc tgttcttttt gcgggaaaac ccaagaagaa gtgaaaaaga
    15421 tcgtagctgg tccaggggtc tacatctgta atgaatgtat cgacctatgt aaagaaatca
    15481 ttgatgaaga attttatgaa gaagctgtac gcgaattcac tgaagtccct aaaccactag
    15541 aaattcttga agtattgaat aactacgtga ttggacaaga tcgcgcaaaa cgctcgttag
    15601 ctgttgccgt atacaatcac tataaacgtg tcaatcagca agcacaagaa aacacagatg
    15661 atgttgaact gcaaaaaagc aatatctgct tgatcggacc tacagggtct ggtaaaacat
    15721 ttttagcaca aacattagca aaaacattga atgtaccttt tgcaatcgca gatgcgacaa
    15781 gtttgacaga agctggttat gtgggggaag atgtagaaaa cattttgctt aaattgcttc
    15841 aatctgctga ttacaatgtg gaacgtgcag aaaaagggat catctatatt gatgagatcg
    15901 ataaaattgc gcgtaaaagc gaaaatgtat ctattactcg tgacgtttcc ggcgaaggtg
    15961 tccaacaagc cttgctgaaa attctagaag gaactgttgc aagcgtgcca cctcagggag
    16021 gacgtaaaca tccacatcaa gaatttattc agattgatac gacgaacata ttgttcatcg
    16081 taggtggtgc atttgatggc atcgaaacga ttgtgaagaa tcgtatgggc gaaaagacga
    16141 ttggatttgg tacacaaaac caaaaacttt ctgaagacga aagtgtcatg cagcatatca
    16201 ttcctgaaga tttactaaaa ttcggattga tccctgagtt tatcggacga ttacctgtta
    16261 tggcagcttt agagaaattg acaacagatg atctggtacg gatcttgact gagccgaaaa
    16321 atgcgctagt caaacaatat caaaaacttc tttctctaga taataccgaa cttgaattcg
    16381 aacctgaagc gttgagagcc attgcgaaaa aagcaatcga acgcaatacc ggtgctcgag
    16441 gacttcgttc catcattgaa gaaatcatga tggatgtgat gtttgatatc ccttcaaatg
    16501 aaaacatcga aaaagtgatt atcacaaaag aagcagctga attgtctggt gagccaacag
    16561 taatctataa agattcagag aaaaaagcgg gttgatgaaa agaggctgcc gtaaaagtca
    16621 ttttggcttt tgcgacagcc gcttgcttta ttcatcacct tgttaaggag agagaccatg
    16681 aaagtccatc aagctgaaat tatcataagt gcagtggcac caaaacaata tccagataca
    16741 caattgccag aaatagctct ggcaggacga tcaaatgtag ggaaatcttc ttttatcaac
    16801 acattgattg atcgaaagaa tttagcccgt acttccggta agccaggaaa gacccagaca
    16861 ttgaattttt atttgatcga gaacgcatta cattttgtcg atgttccggg atatgggtat
    16921 gcaaaagtat ccaaaactga gcgagcaaaa tggggaaaga tgatcgagac atatttaacg
    16981 actagagacc agctacgtgc ggtcgtttct ttagtagatt tgcgtcataa gccttctcaa
    17041 gaagatatcc agatgtatca atttttgaag tattatgata tccctgtaat tgttgttgcc
    17101 actaaggcag ataagatccc tcgtggtaaa tggaacaagc atgagagcga aatcaaaaaa
    17161 gcgttggatt ttgatcgcaa tgacacgttt attctctttt cttccgttac aaaacaggga
    17221 aaagaagaag cgtgggcagc aatcgaacaa gcaattgaat cataaaaaga ataaaaaaga
    17281 cttaccagtc aaactctttc ataaaagttt ggctggtaag tctttttttg agcttaactt
    17341 acttttgttt cttgtctttt ttcttttcat tttgtggctg tggttttaga aatcgatcga
    17401 tctcttcttt tggtgcaact gttttttccg gatcgacagc aaatgattcg aaaagtttat
    17461 ctaaattatc tgtacgttca aatttcaacc ccattgtaaa cacctctttg tttcttttta
    17521 cttagtctat catgtaatcg tccaaattgg tttctaagac ataaagtttt agtttttctt
    17581 tatcgttggt tataattaaa ttataaaaag attttaaaga ggtgagaaaa tgattggcga
    17641 agataaatta ttgatgacta tccagctcac agacttaaat gaagaagaaa aaaaacagga
    17701 aataagcgat tgggctattt tgacacgtct gttgatccag ccaacattta tccgctcttt
    17761 cacacaacaa gcggatccgt atgatcttcg aaagcttcaa gaaaaaataa tgctagcatc
    17821 ggtaagagac gaaagttggt tagttgtagg aaatgacgag aacgaatgta gttttcgctt
    17881 ggaagataat caattattga taaaaaatgt attgtctatc tctgttttca aagaaaagca
    17941 atttttgatt cgagacttca ttcagaaaaa aatgattgct catggggtgt tcgcctatat
    18001 gcgagcttat agcgagttta tctatcacaa tacaaaacag atctctcaac ggctattgtt
    18061 tgagaaaaag gatgaaatcg aacatttacc taaaatgaaa caacaaaacg gggaagtagt
    18121 agtcgattgc aaccaatttc caggatacga tcttttttac caaggtttat gtttcacttc
    18181 gtgttgggaa atgtattata gtcgctatta tcatcaaatc atccctaaac cgatttttct
    18241 cgacatccaa caagtagaaa aagtgaagga attagaaaac gaagtcatat gtatccagct
    18301 ttatcgagac ccctttaact ggcaaaagcc aaacaatcaa atgtttcaat cctattttag
    18361 ggaccagctt gggtttgatc atcttgcttg ggacaatggt gtcggtttat tgaaaccgcc
    18421 attcgttgaa tacatttaca ctgaccatac gatccaatct gtccagtatc agaatgcaca
    18481 aatgcaacct gttcctaaaa agaatgcttc attttttgta acaaaaagtt atgatattca
    18541 acaaggagat tataaagaaa ggcgtgttcg cggaacacta aatgctcagg cgtattttcc
    18601 ttgggttgac gagaatcgct cgcgcatgat gtgctataaa gttatcgatc cgactgttgc
    18661 tttggataat ggaatcgaag cttattgtta ttatattagg gaatatctag aagtagaggt
    18721 aacagatgaa aaatatcaag agtatctttt atccttaaga atctatgttc cttcagaatc
    18781 cctgtcagaa ttaccgttaa aggaaatcaa acatagactc tctgatgtta ccttcaaacg
    18841 aataaagaaa aagcggcgaa gtatccaatt cgatgtcaag aaaggtgagc agcatctgcg
    18901 tgtgcaattt ttagattacc gagaattaga acaattgatt actttacaaa aaatatgagg
    18961 tgaagaaaat ggataaacaa ctctattatc gaataatggt tccggcatca ttattatccg
    19021 tctgtctctt ttttgttcct aattttccta ttcgatcagt catcggtttt ttggtcatgt
    19081 ttggcgggat gggactttat tattatcttc ttcataagga aagaaaacct gcttctgttt
    19141 a
//
LOCUS       DAYYME010000046        18695 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000046 DAYYME010000000
VERSION     DAYYME010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.046"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..787)
                     /locus_tag="ACHBFW_001342"
     CDS             complement(53..787)
                     /locus_tag="ACHBFW_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM9317655.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="ACHBFW_001343"
     CDS             1114..1524
                     /locus_tag="ACHBFW_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFM9317656.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="ACHBFW_001344"
     CDS             1517..2218
                     /locus_tag="ACHBFW_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFM9317657.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="ACHBFW_001345"
     CDS             2218..3804
                     /locus_tag="ACHBFW_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9317658.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="ACHBFW_001346"
     CDS             3823..4044
                     /locus_tag="ACHBFW_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFM9317659.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="ACHBFW_001347"
     CDS             4041..4802
                     /locus_tag="ACHBFW_001347"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFM9317660.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="ACHBFW_001348"
     CDS             5182..5691
                     /locus_tag="ACHBFW_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM9317661.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="ACHBFW_001349"
     CDS             complement(5766..6305)
                     /locus_tag="ACHBFW_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFM9317662.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACHBFW_001350"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACHBFW_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFM9317663.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="ACHBFW_001351"
     CDS             7318..8169
                     /locus_tag="ACHBFW_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFM9317664.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="ACHBFW_001352"
                     /pseudo
     CDS             8197..8832
                     /locus_tag="ACHBFW_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            8852..9625
                     /locus_tag="ACHBFW_001353"
     CDS             8852..9625
                     /locus_tag="ACHBFW_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9317665.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="ACHBFW_001354"
     CDS             10033..10830
                     /locus_tag="ACHBFW_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFM9317666.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="ACHBFW_001355"
     CDS             10808..11221
                     /locus_tag="ACHBFW_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM9317667.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="ACHBFW_001356"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="ACHBFW_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM9317668.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="ACHBFW_001357"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="ACHBFW_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFM9317669.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="ACHBFW_001358"
     CDS             15998..16558
                     /locus_tag="ACHBFW_001358"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFM9317670.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACHBFW_001359"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACHBFW_001359"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFM9317671.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
BASE COUNT         6018 a         3426 c         3995 g         5256 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aataaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttt
//
LOCUS       DAYYME010000047        18505 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000047 DAYYME010000000
VERSION     DAYYME010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.047"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACHBFW_001360"
     CDS             99..746
                     /locus_tag="ACHBFW_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM9317672.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKLLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACHBFW_001361"
     CDS             1139..3784
                     /locus_tag="ACHBFW_001361"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFM9317673.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACHBFW_001362"
     CDS             4094..5407
                     /locus_tag="ACHBFW_001362"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFM9317674.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACHBFW_001363"
     CDS             5397..6050
                     /locus_tag="ACHBFW_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9317675.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACHBFW_001364"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACHBFW_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFM9317676.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACHBFW_001365"
     CDS             6947..7147
                     /locus_tag="ACHBFW_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9317677.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="ACHBFW_001366"
     CDS             complement(7401..9080)
                     /locus_tag="ACHBFW_001366"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM9317678.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="ACHBFW_001367"
     CDS             complement(9082..9294)
                     /locus_tag="ACHBFW_001367"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFM9317679.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="ACHBFW_001368"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="ACHBFW_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFM9317680.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="ACHBFW_001369"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="ACHBFW_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFM9317681.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="ACHBFW_001370"
     CDS             13269..13466
                     /locus_tag="ACHBFW_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317682.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="ACHBFW_001371"
     CDS             13619..14104
                     /locus_tag="ACHBFW_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFM9317683.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACHBFW_001372"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACHBFW_001372"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFM9317684.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="ACHBFW_001373"
     CDS             14981..15508
                     /locus_tag="ACHBFW_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317685.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="ACHBFW_001374"
     CDS             15599..16336
                     /locus_tag="ACHBFW_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM9317686.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="ACHBFW_001375"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="ACHBFW_001375"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFM9317687.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="ACHBFW_001376"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="ACHBFW_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFM9317688.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5983 a         3201 c         4022 g         5299 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag ttgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgc
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaac
//
LOCUS       DAYYME010000048        18470 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000048 DAYYME010000000
VERSION     DAYYME010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.048"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(183..1475)
                     /locus_tag="ACHBFW_001377"
     CDS             complement(183..1475)
                     /locus_tag="ACHBFW_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9317689.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(1472..2095)
                     /locus_tag="ACHBFW_001378"
     CDS             complement(1472..2095)
                     /locus_tag="ACHBFW_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317690.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(2098..2934)
                     /locus_tag="ACHBFW_001379"
     CDS             complement(2098..2934)
                     /locus_tag="ACHBFW_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9317691.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(2951..3838)
                     /locus_tag="ACHBFW_001380"
     CDS             complement(2951..3838)
                     /locus_tag="ACHBFW_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9317692.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(3854..5137)
                     /locus_tag="ACHBFW_001381"
     CDS             complement(3854..5137)
                     /locus_tag="ACHBFW_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9317693.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(5820..6503)
                     /locus_tag="ACHBFW_001382"
     CDS             complement(5820..6503)
                     /locus_tag="ACHBFW_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM9317694.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(6521..7138)
                     /locus_tag="ACHBFW_001383"
     CDS             complement(6521..7138)
                     /locus_tag="ACHBFW_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM9317695.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(7148..7972)
                     /locus_tag="ACHBFW_001384"
     CDS             complement(7148..7972)
                     /locus_tag="ACHBFW_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFM9317696.1"
                     /translation="MNKFLGFRAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(7965..8525)
                     /locus_tag="ACHBFW_001385"
     CDS             complement(7965..8525)
                     /locus_tag="ACHBFW_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317697.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            8699..9655
                     /locus_tag="ACHBFW_001386"
     CDS             8699..9655
                     /locus_tag="ACHBFW_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9317698.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(9960..10916)
                     /locus_tag="ACHBFW_001387"
     CDS             complement(9960..10916)
                     /locus_tag="ACHBFW_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFM9317699.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            11020..11622
                     /locus_tag="ACHBFW_001388"
     CDS             11020..11622
                     /locus_tag="ACHBFW_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM9317700.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(11710..13209)
                     /locus_tag="ACHBFW_001389"
     CDS             complement(11710..13209)
                     /locus_tag="ACHBFW_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFM9317701.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(13225..13749)
                     /locus_tag="ACHBFW_001390"
     CDS             complement(13225..13749)
                     /locus_tag="ACHBFW_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFM9317702.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            14047..14796
                     /locus_tag="ACHBFW_001391"
     CDS             14047..14796
                     /locus_tag="ACHBFW_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM9317703.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            14787..15572
                     /locus_tag="ACHBFW_001392"
     CDS             14787..15572
                     /locus_tag="ACHBFW_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9317704.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            15541..16257
                     /locus_tag="ACHBFW_001393"
     CDS             15541..16257
                     /locus_tag="ACHBFW_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9317705.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(16359..17378)
                     /locus_tag="ACHBFW_001394"
     CDS             complement(16359..17378)
                     /locus_tag="ACHBFW_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFM9317706.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(17397..18455)
                     /locus_tag="ACHBFW_001395"
     CDS             complement(17397..18455)
                     /locus_tag="ACHBFW_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFM9317707.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         5526 a         3901 c         3249 g         5794 t
ORIGIN      
        1 gttcgggttt aaatagttat ttttattttc tcctatctaa aaaagcagcc cacataaaaa
       61 gaaggcatga caaaaagcat gtcatagccc ctttttccga ataaatagtg ttcaaaagac
      121 agcctatcgg caataaggca agattttata aactacggga agtaatttcc gtaaatctat
      181 tcttattctt ttaagctgac aagctcgtca atcactactg cttttcctga agcaatagaa
      241 tgatttgctg cgattcctgt caaaatcgac aaaataccat ctacgtgagt tgctgcccga
      301 ttccacggat cttcttcttt ttcttcacca aatagatcat ctaataaacg cggatctcct
      361 ccgccatgtc cgcccgcttc ttgttcgacg attacctcat atggctcacc gaacatcgga
      421 tagacatgga tggactgaag aacggctgct ccttcatcct ttttatctcc acctgcattg
      481 atgtaagact tttcgatcac tttcatttcg atccgtcctt tgctgccatt gaaagaaaca
      541 ttgaatcctt cccacggcat atacgcgttt agcgaatacg tcaaaatcgc accagtttca
      601 tatttgaccg ttacgcctag tgtatcttca atagagatat catcggaaaa tacactgcgg
      661 tcacgaatat atccactgtc tttttctgct tctaaataca tttctcgcaa ttcctcattg
      721 gctgacaggt ctatagcaaa tggatcagct ttagctagtt cgcttccata gcatcgactg
      781 taaaatattt ctactccgcg tttctccgca ttatgcatcc cataaaagtt taatccaccg
      841 aatgcaaata ccttttgggg acggctgttc aaccaaaaat tcactagatc aaaatgatgg
      901 gtagatttat gaacaagaag cccgccgcta ttgtgcttat ttcgatgcca tcttctgaaa
      961 taatctgccc cgtgttgcgt atttaacagc cattcgaagt ggatagaaaa cacatcacca
     1021 atcacatcac tttctatcag ttcctttatc ttcgtgtgat acggtgcata acgataatta
     1081 aaagtgactc gcaggcttct tcccgtctgt ttttggaccg ttattatttc ttgagctttt
     1141 tcctcatcga tagtcagagg tttttcgcaa ataacatcat atcctttttt cattgcacga
     1201 atgatataat catgatgcgt tcgatctact gacgtaataa tgacttcagc cggccgtacg
     1261 tcatccagta gttgctcaaa ttgtgatggc atacatgtct ttactttttt ctgtccatac
     1321 ttgttcacaa gtatccgatt tgcataatcc atccttgttt gagatacatc acaaaaaccg
     1381 actagttcac aagtttgatg atacgtagag gcaattgcct cataaaaaaa acgcgctctc
     1441 ccaccaagtc cgacttgtac atattttttc ttcatttaaa gtcattctcc ttttctttta
     1501 agcaaaatac tgtgacaaat ggcagccctg gatacagtaa tgcagtgagt aaaaaatcat
     1561 gctgtataca ccagaacagt ccatacaaaa ggcataaata ccacagaaac cccttgacgt
     1621 tttttgacaa cagccagata ttccttttga tcaaataaac aagactatct gctttatctt
     1681 ctttgcagat atcaattagc gtactaaaaa aactatggga tacaaaaagc aacagacaca
     1741 atagaaaagg aatcagatac caagctccaa tctttcccac taataaatac caatcaactg
     1801 caaaaataat gacaaacagc tgaaacaacg taatgatgaa acacatccgt ttgatttttc
     1861 ctttcctcca acataccttt ttttctgcca agtaatggac agatagataa taagaaggat
     1921 acatcatcaa aaaataaatc agatagacaa cacgagcaga cagcactaat cctaaagtga
     1981 acaaacatcc tagaactaaa gcgaaaagag cattactgat aagactggtt aaaaaaatcc
     2041 gacagacata gtagttgagc ttgtaaagaa acatctcttc tattggcttt ttcatattca
     2101 gcccttcaga ccgcttgtac tgatcccctg gacaagatat ttattgaaga ataagaaaat
     2161 cagaaaaacc ggtagcagcg acaacgtaga catcgcaaac atactcgcgt aagtcgtact
     2221 agatccagcg tctgcaaata atttgatggc aatcgatacc gtgtaagatc tcggcttgtt
     2281 taaataaatc aatggtccca tgaagtcgtc ccatttccaa taaaactgga tgatgatcgt
     2341 cgtgatgatc gaaggtttca gcaaaggaaa aataattttg gtaaagacac tatacttgct
     2401 gcatccgtca ataatcgctg attcatctaa ctctttgggg atgctgacca tgaattgcat
     2461 gatctggtaa atgaaaaatg cttgtccaaa aaaatgtggt aaaatcagcg gcacatacgt
     2521 cccaaccaaa ttcagccgat tgtaaataat aaactgcgga atcaaaatca cttgtgcagg
     2581 aatcatcatc gttactaaca tgacgacgaa ccagaatctt tttcctttga aatcgattct
     2641 cgataatgcg taagcaatac aagtcgagct cagaactgtc cctatcgtcg aaactgcaga
     2701 gatgacaaac gaattcctaa agaaagtaaa aaatgaaatt ccagcaaacc ccgcccatcc
     2761 ttttgcgtaa ttttcccatt gaaaagtctc tggaatcaaa ttcagcgaac cactcataat
     2821 ctcttgatcc gttttaaaag aacctgcgat catccataag accggataaa tcatgactag
     2881 acctaatagc aaaataaaac cgtattgaaa ccatttcgtt actgtattat tcatttcttc
     2941 ctcctctcat tcacacaacg acccattttt ttgatgattt gaaaatcaga aaggtgacag
     3001 ctcccataat caacagcatg atccacgaaa tagcactagc ataccccata gaattaaaag
     3061 aaaaagcttg gttatacaca taaagagcat aaaagttcgt ggcatcattc ggaccacctt
     3121 gggtaatgat aaatgcctgc gtaaaagtca taaacgccgc gattgtctgc ataaccaagt
     3181 tatacaaaat gatcggcgaa agaagaggta acgtgatctt gtaaaacgtc tgccatttgt
     3241 tcgctccatc gatctctgcc gcttcataat acgagcctgg gatctctttg atacctgcag
     3301 caaagatgat cattgccgaa ccgaattgcc aagcagacat caaaacaaga gggatcatga
     3361 ccagtttttg atcgctaaac caggaaagcg caggtgcgcc taatcgttcc aggaaaatat
     3421 tcactaaccc gtttttagaa aacaactccc gccaaactag agcgatagaa atactgccac
     3481 cgatcaaact aggcacataa tacaaagaac gaaagaaagt cgttcctttg atattcctcg
     3541 ttaaaagata agcaatcaat agaccaaaga tcagtttcac aggtacagat gcaaatacat
     3601 agagaaaagt gactcggatg gagttcaaaa aacgggcatc actcagcatc tttatataat
     3661 tatccaagcc gatccatgag actggatttc ccaaacgata atcggtgaac gaataataaa
     3721 gtgacaagat catcggtatc actgttatca atagaaatcc taggatgaat ggagaagcaa
     3781 atacgtaacc gaccacattg tcttgatcca agaaccgttt tagtttttct tttttcatgc
     3841 cttcctcctg ctgctatcct agaatgtttg acgcaaactc ataaagactc tctgctgctt
     3901 cttctgctgt tacttcttca taaatgactt tttcaactaa aagattgtat tggtcttgaa
     3961 tttccggatt caaagcacta tcaaagacat tcacttcgcc gttgtccata tcggaaatca
     4021 tttctaaata atcaaatgtc gcttgcaaag ttgcatcatc tgcacgagcg agctgttctc
     4081 gaatattcga catgattggt acaccacgtt ctccttttaa aatttcattg gcttcttcac
     4141 tgttttgaaa gaaatcaata aacttagcag cttcttcttt atgggctgag ctttttgcca
     4201 tactcagcat ttgcgaagaa cgtaaaggga taccagattc tccatctgct tttctgcgag
     4261 gtggattaat caactgaatc tccatatctt caggagcccc tgacaatatt tctgtcaact
     4321 ggttacttga tagaaacacc attgccgctt ctctatccct aaccgcctga aaagacagat
     4381 ccgtcgtact tgcagctcca ccacctggtt ctggatgggc ccctttgtct accagatttt
     4441 tacgcagctg gaaaaaatct gttagatagt ctggattttc aaatcccaag ctgttagata
     4501 gatcttcccg attataaaaa ttcaatcctt tctcaacttg tggtatttga ataatacagc
     4561 cagctataaa atcttcgaat cttgaaatag catagattcc taattcttca gtgatttgat
     4621 agctgatttg ttcaaattca tcccatgtcc aatttgaagc tggttcaggg atatttaatt
     4681 gttcaaatat atttttattg taagcaatcg caaacgaatt caccccgtta gacagtccaa
     4741 ctaattgccc gtcgtaagtc gtcgttgcta aataggattc atctgcatta gaaatatcaa
     4801 ttatcccttg ctccacatat tcatctaatg gttcaatctg attgatgtat tgaggaaatt
     4861 gattgttcag ttggaaaatg tcccagatat catctgatgc tgctagtgta cctaattttg
     4921 tgtaatatcc ttcataatca taaaattcat aatcgatcgt cacatgggga tttttttctt
     4981 cataaagctc gattactttt actgttgcat cgtgtcggac ttgcgaaccc caccaagcca
     5041 tgttcagcgt gatttttttg tcatcttctt gcccacaagc cgtcaagaag aataacataa
     5101 cacttaaagc taagatgatt ctcattcttt tcttcattct ctgcacctct acttccattg
     5161 ttatttttca atatactgta tttttataca gaacatattt attaaacgat ctttttcaag
     5221 gggaataaaa accaagactt gccttatcgt tctgcctagg atgaacaact caatactttc
     5281 aagaaaaatt agatagtgct gattcttctt tcatcgctaa aaacacttct tgcttcaatg
     5341 atgggaggat aattacgcaa agaaaaaagg atatcagaca actgcccaat atccttgaca
     5401 tagctaaata agcatgcttc ttcgtgtgcg ctcttcccta aaaaacagaa aaataactag
     5461 taattctgta tgaaattagg tacaatatac acgtcgatgc acaagcattg actatggcaa
     5521 cttgattggt cagttcccgc tgacttttca agccccgaca atttagctgc tactaaattg
     5581 tcgggtgcct tttttagttt ttattcgttt ttcattctat agaaagcgct tcaataaatc
     5641 aagacatttt tttgttagaa tgctaataat aacaaaaggt tttaactctt cttatgtaca
     5701 ttacaggtaa aaaaacaaaa aaatttctct tttttttgaa ttctgaacaa atagtactgg
     5761 agtacaaaag acgaggaaga gccactatct gctcttcctc gtcttttgat tctcacctgt
     5821 tattcatgat tctttttctc agtttcccga tatttttcca ttttacgact aaaaagctct
     5881 ccgctacttg gtggcgtata tgtgatggca tttgctcctg cttgaatggt ctcagcaata
     5941 ctttcttccg ttggaccacc tgtcgcaatg ataggaagtt caggatattt tttacgaaaa
     6001 tggcgtactg cttctgcagt ttctttacct gcactgatat tgataatcga aataccagct
     6061 gctaactttt catctatttc tgtatatttc gacgttaccg tactgataac tggaatatca
     6121 actactcgac aaacttcttt tactgtctct aaaggcgtag gaccgttcaa aacgacccct
     6181 aacgaacctt gcgcttctgc gaacatgctc atgtaggaag aacgagcacc ttgtgtcaaa
     6241 ccaccaccta caccggagaa aactggaatg tcagctgcct caataatgct tttagtaatc
     6301 gctggatgag gtgtaaaagg atacacagca attactgcat tcgcatttgt attacgtatg
     6361 atcgcaatat ccgtagtaaa gatgatcgaa cggatcaatt ttccaaatat cttgatcccg
     6421 cttgcttcta aaatgatttc cggtactctt acgatatctt ttctcaattc cgtcatgatt
     6481 tctggcgtaa attgttctgc catcagccac ttctcctttg ctatctccgg tcatactgat
     6541 tcaattgata cgtctcgatg acctggatga ttttttgcgc ataaccgggg tctgtcgcat
     6601 aacctgcatc ttgcaatgct tgtgcggctt cctgatagtt ggtggccgta atcacttttt
     6661 catacagcgc aggattccaa tctactcctt ggacgaacag ttgtgtatgg tcatccatgg
     6721 actgttccca tgaatcatac actttgaagt ctccttggat agttatccat tcttcgttaa
     6781 caaattcctt tgtttccagg cttactttct tctgatcccc ataagccttg atcccgaaaa
     6841 gattattgta tttgctagcc aaggtacttt ttccccaatt tgattctaat atagcttgtc
     6901 ctaaaatgat actaggaagc actccataac catcttgtag ttcttgggca tgcggaacaa
     6961 gccgttcaat aaattgctga tgggattgcg cttcattttc ttgctgatta tcagtaaaag
     7021 gagaagacag attcttcaag gaaaaaacaa aggctaagag tatcactgct acaccggcaa
     7081 ccactgctgg ccaacggata ttcttgcgct tttgcttttt ccactttttt ttcgccatga
     7141 tcaattccta cttttcattt aattcattca gatattcttc tagcgtcaat tggtgtttgg
     7201 tgatatactc agcgttttct ttaccgacat aacgtaagtg ccaaggttca tatgtgatct
     7261 tagttatatc ttcgtgtcct tttggataac gaacgataaa tccatactgc gcagcattat
     7321 ctgcgatcca tttagctcca ggctgatctc cgtaacttgc atccaataca gtagaaggat
     7381 aactgttgta ccagttttga tcgacgacat cgactgccag acctgtgtgg tgttcgctgt
     7441 atcctggctc cgtaatcgtt tctttagtaa tctttgtcgc ctcctcttct gatacacctt
     7501 gacgactcat cacatcttgg acattttgtt cgaaaacttg ttgttgggat gatacagacc
     7561 ggtaagctga gaccatcact aatggaaatc cagctgcttc agcagccttg gcaaatgctt
     7621 catattctgg agcaatgcgt ttgtcgatca agtatccgtt acttaatgat gccaattgag
     7681 tagactcatc aatttctttt tctaatttgt gatctggacc aaccaaaact agcttccaat
     7741 cgtctgtttt aacatctggc aattgtttgg acgtacgatc ttttgtttct ttgtccttta
     7801 ccttgctgct ggttgtttga ctgttctttt ctttactagc agttgttttg gtgcttgttc
     7861 gttcgactgc ttgattgccg gcttgaacat gcgaatcatc atcgctaaaa ataacatagc
     7921 ccattgctgc gatcataatc acaactgctc taaatcctaa aaatttattc acttgaaaaa
     7981 tcctttcgtt gttcttcacg ttccccattc gcctcatgaa cgtaagtttc ttccagattt
     8041 tcattcaccc aatcgagaag ttcgattttt tctccttcga tctgtttttg aacatctgct
     8101 ggcagacgaa aattcaaaat atcatcatag ccccattcat catattgaat acttactttc
     8161 aagtcaaggt atctaccagc ctgctgatta tttttcaaag ggatcagctc gacttttgct
     8221 gctttgctgc taagccattt ttgcgcgatg cgtgttttga tttgcttgta ctctgcaacg
     8281 gcaacgtttc gtttttcggg aaaaataatc gtttgacgca aaatcttttg cgttagcatc
     8341 cattcataat cagtgaaaag attttttact ttctgcatcg cttcagatgg cagatttttc
     8401 tcgccatctt tccatttttg ccattgttct ggactgatca gcaagtattc atctaaaaaa
     8461 tttttttcag tttcaaactg ttctaataat acactcataa taagatcaat atatacttga
     8521 tgcacgttta tcccctcctt ttaactattc tacttaagtt ttgatcaaaa agcacctctt
     8581 aaatcgctgc tttcattttt tttaatgttt caagtcctct cctctttatt cttcgtgatt
     8641 tttgttataa taatagacag acaagaaact caatccaact atgaaggagc gttttcagat
     8701 gaaaaaatat caatggggaa tcgttggtct tggtaccatc gcacatgagt ttgccgaaag
     8761 cttcaatcaa gagacgagcg aactaacagc agttgcttcc agaacttcag aaaaagcgga
     8821 aaacttcgct caccgttaca atattccaaa agcttacgga agctatcaag aaatgttaga
     8881 cgatgctgaa atcgacatcg tgtacattgc tgtaccaaat agacagcata tcgaccatat
     8941 cttggcagca ttggaagcag gcaaacacgt tctatgtgaa aaagccatca cgatgaataa
     9001 aaaagaactg gctgacgcta tgcgcttagc agaagaaaag aatttgatct tagcagaagc
     9061 gatgacaatc ttcaacatgc cactttatca gcagttacgc tcaataatgg atacaggaaa
     9121 actgggggct ttaaaaatga tccaagcacc tttcggcagt tacaaagaac cagaccctaa
     9181 aaaccggttc ttcaatccag aattagctgg aggagctctg ttggatatcg gtacgtatgc
     9241 tgtatctttc gctcgtttct ttttgagctc tcaacctgaa gtagttgcct ctacaatggt
     9301 tccttttgaa acaggcgtgg acgaacaatc cgtcactatc ttgcgtaaca aagaaaatga
     9361 acttgctgct gtcagcttga cttttcaggc aaaaatgcca aaagtcggta tcgttgcctt
     9421 tgagaacggt tatgtgacga ttgctgatta cccgcgtgct gatcgtgcgg agatcttatt
     9481 tacagatggt acaaaggaat tcatcgaaag cgggaataca tctgaagcaa tgaattacga
     9541 aataaagaat atggtacaca tgattgaagg tgacttgccc aatcgctctc tcttcttgac
     9601 aaaagatgtc atcgaaatct tggatcaaat gcaagttcta tggaaaaata tgtaatttta
     9661 aaaggttgtg aaaaagctgt tctgcatcaa gtaataagaa cagccaaaca aaaatagctc
     9721 ctcctatttt tcgtttggct ggacttatta ccgcagagga aagcttttga acaccgttta
     9781 agaaagaggt tgtgagccta ccgttcagct ccgagtcaca aggagcaatt tcaaaaaaca
     9841 gcttctcatg tttgttgaat attgtgactt gtgaccgagg agttgacagg agaacaccgt
     9901 ttaaagaaga ggttgtgaaa tcagcttttt gacctgaata ttagctgaat tcacaacctc
     9961 tatttttgga ttttttgttg ggagaattct gccttgattt caataaaact tttaaaagac
    10021 tgcaaaaact ggaacaattc tgctcgatcc tcgaattcag ggcgactagt cggcagaggc
    10081 atttgccgat acaatacata aacttcttca atacgtgtta aaatttgtct gccatcattt
    10141 gactctgaaa atgtttctgc agtataaatc aataaaccat agatatgttt tccataataa
    10201 gctggttgct ggattctctc tagattttcc agcatgtctt taatcacatt ggcctgtgct
    10261 cttctcatag agaagtaagt ttcgtagtat agcggctgtc tgagccagta gttttcttga
    10321 tgttctctgg catcttcttg gctttcgcga atgaatgtca gtaaatgttc gcatttgatc
    10381 tgcacatctt ctttattttc tgataaaaga aattctgcca tttgtctcaa aatatttcga
    10441 aattcttttt cgatctgtat ttggttttcc ttcagctgct tcgtacgatc tggcatgtaa
    10501 acatttgcaa gcaaagcaaa tactaatcca atcgccatta atcccgcttc attgccgatc
    10561 agctgccaag acatgttttc ctccactaaa taatgagtga ccaaaaccga atttactact
    10621 atcccttcgg ttaatttgaa gcgagaggca agcgggatga acaggaataa aaaaagactg
    10681 aagccaaaaa tagtataccc aacagcaaaa aaactgaaca gtgctgttgc cgtagctaat
    10741 ataaaggctg cgattcgatt catgcccgta cgcaatgtag agcgcttggt atttcctaca
    10801 cttaataatg caatgatccc tgcagctggc caatacaaca gcgataagct acccgctgtg
    10861 accatcgaaa agaagacact gatcactgtt ttgactgttc gcattcctat acgcatttac
    10921 ttcccccttt tttattttaa ttatacttga ataatgaaaa actagctatc agacaaaagt
    10981 ttaggtatga tagtaaaaag accaaaaagg agaatctcta tgcaatttgg ccgttttcga
    11041 ctaggtatgc gtacgatcaa aagtgcctta gctgtttttt tatgtatctt gttttttcat
    11101 ttgactcaac gaggaatccc gatgattgct gcattatctg ctgttttttc tttgcgtcag
    11161 gacttgacta caaccgtttc ttttggaaag tctaggatca tcggcaatac attaggtggt
    11221 acattagcta tcatttattt ttttgtcaaa agctattttc acaatgattt tttggtagaa
    11281 ttgatcttgc ttcctattct agttattatt gtgatcgtcg tgtcagatgg tattaacaat
    11341 aattccggta tcatttccgc aatagctacg ttgttgctga ttgcgttgag catccctcaa
    11401 ggcgaatccg ctttgtatgc tatccaacgt gtactagata cttttatagg gacattcatt
    11461 gcaatcggta tcaatttctt tcttcgtcct cctgaaacag aaaagaagga agaactatca
    11521 gaagatttgg tcgaactcca aaagaaagaa cgattgctaa gaacaaattt acaagagata
    11581 gaagctaaga tccaaaaaca aaagaaaaat agcgaggact aattttcttt tgcaataaaa
    11641 taaaaaaatg gagagaagaa ctttaaaaag tcttcctcca tttttgattg aacggtgaag
    11701 tgtactactt tattcttcct tcatcgagtt ttgttctgcc tcttttggcg cttcatacat
    11761 atttacttgg ctagtagtcc cacgtgtcga caaattctct acgatttcct tcaagtcaag
    11821 tcctgttgtc gcttttagtg tttcttgagt agaagaaagc aaatttgtgg cataatttgt
    11881 cactcggttc gctccactgg tttctcctga actgcttcct gtatcgacaa cagagatttt
    11941 ttcgatattt cctagcggct gtgctgcttc gcgcatcaat tgcggtagca tatcgataac
    12001 catactcaag acagctgctt cgccatattc cttgaaggca ttggcgatct tttgttttgc
    12061 ttcggcttct gcttttcctt ttgctagtgc tgcttcggct tctgcttgtc cagctaaacg
    12121 tgtcttattg gcttccgctt ctgccagtgc ttccacacgg aatcgttctg cttcggcttc
    12181 agtcacttct ttgacttttt gagcttgtgc ttcttgttct ttagcgtacc gatcggcatc
    12241 tgcttttttc ttcacttccg aatcatattg cttttcacga cgtgtgattt ctttttcttc
    12301 cagttcgatt tgtttttgac gttctacgat tttaatctgc atttcctgtt cgattacttg
    12361 ttgttgtgct cgggcacttt ctaaattgta ggcttggtct gcttttgctt tagcaacatc
    12421 ttgttcttgt ttgtaggttg ctaatttcag ttctttttct tttaatgatt ctgcgatctc
    12481 tgtttgtcgc tgtaattcag ctgcttgtga ttctttttct gcttgagctt ttttgatacg
    12541 cgtttctttt agagcttccg cttctgctat atctgcatct cttttgactt gggcgattct
    12601 tggtttccct aaagagtcaa gataaccgtt tttgtctctg acctctttga tagtgaaaga
    12661 aacgatgatc aaccccattt ttgcaaggtc tacagaagcg acttcttgta cgctttgact
    12721 gaacttatcg cgattttgat agatttcttc tacagtcata gaaccaagaa tcgagcgcaa
    12781 gtgtccttct aatacttctc ttgcttcatt ttccagttct tctcttgtct tgcctaaaaa
    12841 ctgttctgct gctgtagcaa tctcttctac agatgaaccg attttgatga tcgaagtacc
    12901 atcacacatc accggtacgc cttgttccgt atacacttca ggggtggaga cgtccaattt
    12961 actcgataat agactgatcc gattcgaacg ttgaaaaact ggcaagacga atgcgccacc
    13021 tccacgcaca attttgattt tgttattgct ttcatcaatg tgcacatttt tgcttcctaa
    13081 ataacttcca ctgatgatca acgcttcatc cggttttgct gtctggtact tcgctacaaa
    13141 gacgatcagc aacatcagta aaatcaaagc tgcgattgcg atcgcgataa aaacaactgg
    13201 tatttccatt taacacacgc tcctttaagc ttttatcatg ctttcataag gcatgacata
    13261 acagattctt tccttgatct caatgatcaa tactttcgtt ccccgctcag ctgtctgttc
    13321 ttgggggctg taaaaacaag ctggacgact aatggaaccg gtaacacttt tcaactgaat
    13381 ttctcccata cctcgtacag ggatcggtgt gatcacaaca gctacttgtc cttccagagt
    13441 tttttcagaa gacgacatcg ttgattcggc atgtttcatc ggcatcaata tatagaaatt
    13501 aagcaaaaat accaacacta tcgatatgcc tccactagca ataaataaaa ataaagatga
    13561 ttgatctgtt aaacgctcac ctagaaaacc aaataagctt gtgaaagtta accaagggat
    13621 aataagcatc ggatcaagcg ggccatcaaa atcgaataca tctccaaaaa taatcaatag
    13681 gactgccaat cctgcacaaa tcagcaatgt ataccaataa atcgtctcaa gtgcgattcc
    13741 ccctatcata tacacgcctc cttccatttc tacgtctatc ataccaatgt ttgaaaattc
    13801 tgtatagctt cttttaccga ctcagtggtg ttttttatag gaaggttgtg tttatcgttg
    13861 acaatttgtt aaatttttgt tttcatgtta aaattttcac aataaattct ttcgaggtag
    13921 cctatgtttc ctttttttaa gaaaaaacca ttaaagctcc agaacacgat catccttgtc
    13981 gtttgtttct ccacattcgt ttctttgatt gtttctgcca tattgatcag gaattttatt
    14041 ctttcgatga accgatctag tgtgtcagat aaaatctcta gcattgctaa aatcgttgcc
    14101 aatgataaac tcatccaaaa aaagctgcag actggtgacc cgacacatgc gattcaagga
    14161 ataacaaatg aaatcatgat tgcatcgaat ttagatttta tcgttgtcat ggaccctgaa
    14221 ggcattcgat attctcacgc agacccaagt tacatcggtc ttcctttctc aagtcatgaa
    14281 gatgtgaaaa aagtgattgg aggacaagaa tttttctctg aaaaagaagg aatgttaggg
    14341 ttcggcttcc gctatttcgt ccctatttat tatgatcaag agattattgg tgcagtttgt
    14401 gttggcttaa caaacaacac gattgacaat caagtcaaag atggacaaaa aatgattgtt
    14461 ttttcattga ttattggact tagcgtaggc atcataggcg ccgtttttct tgcccaaaaa
    14521 atgaaaaaag tactgcttgg acttgaacca caagaaatag cagaacagtt aagtgaaaaa
    14581 acgattatcg aaaatgaagt agatgaagga atcttagcca tctcttctga taaaactatc
    14641 ctcttgatca atacacctgc aaaaaacatt ttctaccaaa cagagcagct attggatttc
    14701 gaagtaggaa aaaaatcagt gataatctat atgacgtttt gtttaaagag acgattgagc
    14761 atcaaacgac cgttcggact caatccatgt atttaaatgg gatagaaact atttctagcg
    14821 ttacacctct ttttagcaat gaccgttttt taggagcagt cgcaacttta cgaaatcgat
    14881 cagaagtaat gaaattaatg gaggagcttg gggggacgaa acaatatata aactccatga
    14941 gagctcaaac ccatgaattc atgaataaac ttcatgtcat ttctggtttg attgatttga
    15001 agaagtatga agaagtcacc cattttatcc agcagctaaa tcaaaattat aaaaaagaga
    15061 ttggccaagt ctcaagtctg atcaaagtac ctgcaattgc cggtttttta ttaggcaaaa
    15121 taaacgaagc caaagaacaa tctgttgaaa tgctgttaga aagcgattct tttatcccta
    15181 aaatggagat ggatgactcc atccattcac ttttgcaagt gttagggaac ttacttgata
    15241 atgcaaaaga agccgtgcta caaaaacatg aaagaaacgg acaggtccgg attcatttaa
    15301 actatgacac agaagctgaa gtattcatcg tgaccgtctg cgatgacggt gtcgggatat
    15361 cagaagaaat ctcagaaaaa atatttacgc taggtttttc gacgaaggga atcaatcgtg
    15421 ggtttggatt gaatctcatc cattcaatcg tcaaacgcca cggtggattt ttagaatttt
    15481 ccaaacaaac aaatggcggt acaatcgtct atctagaatt accaaaaaag gaagatcaaa
    15541 atgatgacaa atgtcctgat aatcgaagat gatccaatgg ttgcagaatt gaatcgtact
    15601 tttgtggaaa gcatccctaa tatgaacgta atcgaaagtt tcagaaatgt agaagaagga
    15661 aaaaaatatt tcagacaaaa aaacgcagat ctgctgctgc ttgatgtata catgcctggc
    15721 gaaagtggta tccatttttt atcttggctt agacagcaag gaaatgatat cccagtgatt
    15781 ttgatcactg ctgcagatga tatcgaaacc ataaagaatg tgatccgcta cggtgcctta
    15841 gattatttga tcaaaccgtt cactttgcaa cgttttcagc tggcgatcga aaagtatctt
    15901 gctttctctc agacaatcca aacgactgaa aaagccgatc agaaattgat cgatcaagta
    15961 ttcaaccaca ccactcccat tcacgaaata ccatcattca tcgatttgcc taaaggtctg
    16021 tcaaaactga ctttaaaaaa aataagtgca tttattctaa aagaagatac tattttttca
    16081 acaaaagaac tggctcaaaa aagctcactg tcacgtatat ccgtcaagaa atatttgaat
    16141 tttttagtag aacaagatat attgaacgaa agaatcaaat atttggaaat cggccggccg
    16201 ctaacctgtt atgaattgac aaagaatggg cggaaaaagc tcacagatta ctgttgagtc
    16261 atcgaattat cccatacaaa atagtccaag tatacagaag taacaactgt ttacttggac
    16321 tatttttaaa cattttattg acgaatatcc atgttttctt atcttaattg acgggcttta
    16381 ggaagagata cctcattttt cgtttttcca gtttctatta tttcacgaaa gttctcgaaa
    16441 cttgtcgaaa tcatatttga taatgcctca tctgtatttg aacctacatg cggggttaca
    16501 agaactctag ggtacatttc tactaattgc tggatagcag ggtctggaat cgtctcccat
    16561 ggcttgaagg atcggaaaaa aacctctgtt tcatttgcaa aaacatcggt tgcaaaacca
    16621 gctaaatgat tgcttttcaa agctcttaaa atagcttcat tatcttgaag ctctccacgg
    16681 gctgtattaa tcaagatgct accttttttc atttgagcta aaaatccttc attgatcatt
    16741 tgatcgtttt cacctgggat atgaggtaca tgaatactga caatatcgct tttggctaat
    16801 aactcttcaa gcgagtggaa cgtcaatact tttttagcag cttctgtctg atgaatatcg
    16861 tagccgatca ctttggctcc tagtccctta aacaaaacag cttccgtcaa ccctatcctg
    16921 cctgttccta tgatccctac tgtgcaattt ctgatttcac gactaaacat ttgctcatcc
    16981 actcgaaaat ctttgaaaga tgttcggatc gtcgtatacg cagtatttct caaaagcatc
    17041 atggcaaaag tcaaagataa ctctgctatc gcatttggcg aatacgatgg tacacgggct
    17101 actgtcatac caaaatcagc tgccgcttgt agatcgatat gattgactcc cacagtccgt
    17161 gtaaaaacat aacgaatccc atattcctgc atttttgcaa gattggtctc atctgctata
    17221 caatttcccc tcaacaatac agcatcatgg tcctttgctg acacgatatt gtcatgtgtc
    17281 aacagttctt cataaagact gagatcataa tggtaaatat tcaatttttc aaaaaaatct
    17341 atctcgttcg gacgaacacc gtaacatgcg attctcatac tttcttcctc catttcttat
    17401 gaaaataaac ctatgccatg aatcatttgt aatacaacga tccatacagg tgtcagtacc
    17461 acagcaaaaa tagttgacag taaggatgca ttggaggcta aaagtgcctc cctatcaaaa
    17521 ctgatagcat aggctgctgc cactgttgca gctggtgtag ccatcatgat aacgattgtt
    17581 gccagtgctt caaaattgac tggtagaata ttcgttgccg tcaaaactgc tagaatcacg
    17641 atattcaatg caggtacaaa taccactttg actagactgt aataccatga agttttttca
    17701 gatgcagctt ttttgaagct tacttcacct aatgttgcgc cgattgctag ccacgccaat
    17761 ggtgaactta aaccagctaa ataatccatc ggctgaaata gccacggtgc agtctggtca
    17821 attcgcaaaa acgctacttg gcttatctct ccttctgcac ctgtaactga cacttgtggc
    17881 agataccctt ggaacagcca aatgaaaagg ccggcaaaag tagcgataat gattggattg
    17941 acgaacatcg ctttgatatt tttagcttcc atcttcaagc cactcatttt tatgtatcca
    18001 taagaataca aaaagattcg gtagccaata ttgaaaattg aggaatacat cacgccgaca
    18061 ggaccataaa tcgcactgac gataggcgtt ccgaaaaagg tcgtagaacc aaagatcgtc
    18121 aggacacgca acgttgtttg cttatcccct ttatagctta agaaaaaagg ttttgaaact
    18181 agaataagaa taatataaat aactaatccc caaatcaaga cattgattcc ttgcatcagt
    18241 aattctgtat tgatatcttg catgaaagct cgaaatgcca gtgctggcaa agccactgtt
    18301 aagacgactt tcgatagaat ttttccaaca gattcagaaa aaatcccctt ttttctacaa
    18361 acaaacccta ataatataat aaaaactgtt gaagtgattg cactgacaat cgacatatct
    18421 gtcaaagtcg atcgaaaaat gtcaaataag ttcatctttc tcctcctaat
//
LOCUS       DAYYME010000049        18130 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000049 DAYYME010000000
VERSION     DAYYME010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.049"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..2714
                     /gene="adhE"
                     /locus_tag="ACHBFW_001396"
                     /gene_synonym="adhC"
     CDS             117..2714
                     /gene="adhE"
                     /locus_tag="ACHBFW_001396"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFM9317708.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2943..4049
                     /locus_tag="ACHBFW_001397"
     CDS             2943..4049
                     /locus_tag="ACHBFW_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM9317709.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4066..5094
                     /locus_tag="ACHBFW_001398"
     CDS             4066..5094
                     /locus_tag="ACHBFW_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFM9317710.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5100..5579
                     /locus_tag="ACHBFW_001399"
     CDS             5100..5579
                     /locus_tag="ACHBFW_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFM9317711.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5595..6890
                     /locus_tag="ACHBFW_001400"
     CDS             5595..6890
                     /locus_tag="ACHBFW_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFM9317712.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6901..7725
                     /locus_tag="ACHBFW_001401"
     CDS             6901..7725
                     /locus_tag="ACHBFW_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM9317713.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7890..8789
                     /locus_tag="ACHBFW_001402"
     CDS             7890..8789
                     /locus_tag="ACHBFW_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9317714.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8915..9784)
                     /locus_tag="ACHBFW_001403"
     CDS             complement(8915..9784)
                     /locus_tag="ACHBFW_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM9317715.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9937..11397
                     /gene="rhaB"
                     /locus_tag="ACHBFW_001404"
     CDS             9937..11397
                     /gene="rhaB"
                     /locus_tag="ACHBFW_001404"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFM9317716.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11394..12650
                     /gene="rhaA"
                     /locus_tag="ACHBFW_001405"
     CDS             11394..12650
                     /gene="rhaA"
                     /locus_tag="ACHBFW_001405"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFM9317717.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12664..13509
                     /gene="rhaD"
                     /locus_tag="ACHBFW_001406"
     CDS             12664..13509
                     /gene="rhaD"
                     /locus_tag="ACHBFW_001406"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFM9317718.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13511..13822
                     /gene="rhaM"
                     /locus_tag="ACHBFW_001407"
     CDS             13511..13822
                     /gene="rhaM"
                     /locus_tag="ACHBFW_001407"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFM9317719.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13904..15055
                     /gene="fucO"
                     /locus_tag="ACHBFW_001408"
     CDS             13904..15055
                     /gene="fucO"
                     /locus_tag="ACHBFW_001408"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFM9317720.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            15182..15940
                     /locus_tag="ACHBFW_001409"
     CDS             15182..15940
                     /locus_tag="ACHBFW_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFM9317721.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            16040..16684
                     /locus_tag="ACHBFW_001410"
     CDS             16040..16684
                     /locus_tag="ACHBFW_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM9317722.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            16691..17698
                     /locus_tag="ACHBFW_001411"
     CDS             16691..17698
                     /locus_tag="ACHBFW_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM9317723.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            17714..>18130
                     /locus_tag="ACHBFW_001412"
     CDS             17714..>18130
                     /locus_tag="ACHBFW_001412"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="4-deoxy-L-threo-5-hexosulose-uronate
                     ketol-isomerase; catalyzes the interconversion of
                     4-deoxy-L-threo-5-hexosulose uronate to
                     3-deoxy-D-glycero-2,5-hexodiulosonate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFM9317724.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYP"
BASE COUNT         5906 a         3130 c         4079 g         5015 t
ORIGIN      
        1 atttaaaaat aactgtgaaa aaaatcacaa aaaagtgttt acatagaaaa ataaatattg
       61 tatgatatac atgtgaatta tttaacaaat aaccatgacc cattaggagg ataatgatgg
      121 ctaaagtaat agaaaaagaa aaaacgaaga caattgacgt acaagcaatg attgatgatt
      181 tggcagaaaa agcaaatgca gcattgaaag aaatggaaag ctttgatcaa gaaaaagtag
      241 accatatcgt gcatgaaatg gcaatggcag ctttggatca acatatgcca ttagcgaaga
      301 tggctgtaga ggaaacaggc cgcggtgttt acgaagataa agcaatcaaa aatatgtatg
      361 cgtccgaata tatttggcat aatatcaaac atgataaaac agttggtgtg atcaatgaag
      421 atgtccaaaa agggttgatt gaaattgctg aaccagtcgg tgtggtttgc ggagtaactc
      481 caacaacgaa tccgacttcc acaacgattt tcaaatcaat gatcgcgttg aaaacaagaa
      541 atccaatcgt ttttgctttc catccaagtg cccaaaaatc ttctgcagaa gcagcacgag
      601 tggtacgtga tgctgctatc gctgccggag ctcctgaaaa ttgtatccaa tggatcgaac
      661 atccttcgat cgaagcgact tctatgctaa tgaaccatcc aggtatcgcg atcgtcttag
      721 cgacaggtgg agctggaatg gtcaagtctg cttactctac aggaaaacca gctttaggtg
      781 taggtccagg taacgtacca gcttatattg aaaaaacagc aaaaattaaa cgtgcagtga
      841 acgatttgat cgtttcgaaa acatttgata atggaatgat ttgtgcttct gaacaagctg
      901 tgatcgtgga taaagaaatc tatgcagcag taaaagcaga attccaagca caccaagttt
      961 atatcgtaaa accagatgaa ttgcaaaaat tagaagatgc agtgatgaac gaaggaaaat
     1021 atgcggtcaa tccatcaatc gttggacatt ctgcgatgga aatcgcaaaa ttggcaggaa
     1081 tcagtgtacc aaaaggaacg aaaatgctga ttgctgaatt ggaaggcgta ggcccagatt
     1141 atccgctatc aagagaaaaa ctttctccag ttcttgccat gatcaaagct aacaacacgg
     1201 accatgcgtt tgatttgtgt gaaggtatgt tgaatcttgg aggattagga catacagcag
     1261 ttatccattc agaaaacgaa gagttgcatg tcaaattcgg tcttcgaatg aaagcttgtc
     1321 gtattttagt gaatacacca tctgcagaag gcggcatcgg tgatatctat aacgaaatga
     1381 ttccgtcatt gactttggga tgcggttctt atggtaagaa ctctgtttct cgtaacgtct
     1441 ctgctgtaaa cttaatcaat gtcaaaactg tagcgaaacg gagaaataac atgcaatggt
     1501 tcaaattacc gccaaaaatc ttctttgaaa agaactctct attgtactta gagaaaatgg
     1561 aaaacgttga acgtgtcatg atcgtttgtg accctggtat ggtccaattc ggttactgcg
     1621 atacagttcg tgaagtattg gcacgacgta aaaatgatgt gaagatcgaa gtcttctcag
     1681 atgttgaacc gaacccatcg acaaataccg tatacgcagg aacgaaactg atggcagact
     1741 tcaagccaga tacagtaatc gctcttggag gcggttctgc aatggacgca gctaagggaa
     1801 tgtggatgtt ctatgaacac ccagacacat ctttctttgg tgcaaaacaa aaattcttgg
     1861 atatccgtaa acggacatat aagatcgaca aaccagaaaa aacacaattc gtatgtatcc
     1921 cgacaacatc agggactggt tctgaagtaa cgccatttgc ggtcatcact gacagtgaaa
     1981 cacatgtgaa atatccgtta gcagattatg cattgacgcc agatgtcgca atcgtcgatc
     2041 ctcaattcgt catgtcagtt ccagcatctg tcacagcaga taccgggatg gatgtgttaa
     2101 cacatgcaat cgaatcctat gtttcagtca tggcttcaga ttatacacgt ggattgagcc
     2161 tacaagcaat caagttagtc tttgactatt tagaaaaatc agtaaaaaca cctgatatgg
     2221 aatcacgtga aaaaatgcac aacgcttcta caatggctgg tatggcgttt gctaacgcat
     2281 tcctaggtat ttgtcactca gtcgcacata aaatcggcgg ggaatacggg atccctcatg
     2341 gacggacaaa tgcgattctt ttaccgcata ttatccgcta taatgcaaaa gatccacaaa
     2401 aacatgcgat gttccctaaa tatgactatt tccgtgcaga tacagattac gcagatatcg
     2461 caaaattctt aggtctgaaa ggcgaaacaa cagaggaatt agtcgaagct ttagcaacgg
     2521 ctgtttatga attaggaaaa tctgtcggca tcaacatgag tctaaaagct caaggagtca
     2581 cacaagaaac actagataca accgtagacc gtatggctga attagcttac gaagaccaat
     2641 gtacgacagc taatccgaaa gaaccattga tctctgaatt gaaacagatc atcattgatg
     2701 cttacaacgg ataagcaaaa gaaagcgtag aaaaaagata agaaaataga tgaaaaagaa
     2761 cttggaaaac gttgtttctc caagttcttt ttttaatttc caagtaactg aaagttactt
     2821 ttgttattta acatttaggt caattttaga cagtttttgt tcaaaaagcg atatttatag
     2881 tggacggtat tactgctata ttaaggatat aaaatgaaag cgtttaaaaa ggtggcgtaa
     2941 agatgattat taaagaagag atttttgtac ctaaagaatt agtgaaagaa aaaattgacc
     3001 tgctcgttga aaacctgaca acgatcaaag acgataatgg tgaatttttg ttggactttg
     3061 atggactgaa agtagatgac aaaagctgga cagtgtggaa ttggccacaa ggtgtcggtc
     3121 tatatggtat ttataaaaat taccagatga caaaaaatcc tcgcgcttat caagtggtca
     3181 atgaatggtt tgaggatcgt atggaagaag gcgctccgcc caaaaacgtc aatacaatgg
     3241 caccgctatt gactatggct tacttgtatg aagacacgaa agattcacgc tatttgcctt
     3301 atttagaaca atgggcagaa tgggtcatga atgacatgcc tcggacaaat gaagacgggt
     3361 tacagcatgc cacatatgga ccggaaaata agaatcagtt gtgggacgat acattgatga
     3421 tgacagtgct tcctttggca aaaatcggga aactgttgaa tcgcccagaa tatttagaag
     3481 aagctcgtta ccagtttatg atccatatca aatacttgat ggataaacga acaggattgt
     3541 ggtatcacgg ctggacgttt gaaggaaatc ataactatgc cgaagcattt tgggctcgtg
     3601 gaaactgctg gttgacgatt gctattccag agatcattga aatcttagaa ttatcgaaag
     3661 aagatgcatt gcgcaaatta ttgatcgaaa cacttgaagc acaagtgcgt gcattaaaaa
     3721 cttatcagtc tgaaagcggt ttatggcata cgttgttgga tgatccttct tcttacgtag
     3781 aatcttctgc aactgcagga tttgcttacg gtattttgaa agcggtccat aaacgttatt
     3841 taccacaaga atataaagaa gtcgcttatc gtgctatcca aggattgctt gaacaaatcg
     3901 atgaaaaagg agaagttcaa aacgtttcaa tcgggacagg aatgggggac agtttagatt
     3961 tttatcggaa tatcggaatc acagcaatgc cttatggaca gtcgttaacg gtgctttgtt
     4021 tgacagaatt gctcgtctct tattgttaaa agaaaggaaa cccatatgaa aaaaacatca
     4081 ctgctgtttg cagcaagttc gcttagccta ggtctgctag gtctaagcgg ctgcggaaat
     4141 aattctagtg tggcaactgt cgcagatgca gatgtaaatg agatagttac cttgcgcttt
     4201 gcttatgcaa gcaacagcca gccagtaatt gatgcaatga atgaatttgg gcgtttagta
     4261 aaagaaaaaa cagatggttc cgtacagatc cagtatttcc cagatggaca actgggtgga
     4321 gaacgtgaac tgatcgaatt gacacagtca ggagcagtag atttcacaaa agtaagtgcc
     4381 tcagcgttgg aaagtttctc taaagattat tcgatttttt cagttcctta tctattcaat
     4441 gatgaagaac atttctttaa ggtgatggat aatgaagaaa taatggagcc aatctaccaa
     4501 tcgaccaaaa atttaggatt taccggattg acctattatg attctggtca gcgcagcttt
     4561 tatatggtag acggtccgat ccatacgccg gaagatttga agggaaagaa gatccgtgtg
     4621 atgcaaagcg aaaccgctat caaaatggta gagcttttag gcggttcgcc cgtacctatg
     4681 ggaagtgatg aagtgtacac gtctcttcaa tccaatttga tcaacggatc agaaaacaat
     4741 gaatttgtct tgtatacagc tggacatggt ggtgtggcta aatactattc ctatgatgag
     4801 catacacgtg tgccagatat catcatcatg aatgatgcca tcaaagaaca gctaactgat
     4861 gagcaggaaa aagccattga agaagcagcg aaagaatcaa cggtttttga aattgaagct
     4921 tttgcaaaag caattgaaga agaaaaagct ttagcacaag aagaacatgg agtccaattc
     4981 aatgatgtag ataatacacc gtttcgagag gcagttgccc cacttcatga tatattcaaa
     5041 aacgatccta cctacaaaga cctttatcaa aagatccaag agcaaggagc gtagctgtga
     5101 tgaaaatgat tttaaacaag gtattagaga tcctgggagc tgttttgctt gttgggatgg
     5161 tcgctgttgt tttatggcaa gtattttcca gaacggtttt aggaaatcca aatacggtca
     5221 cggaagagct tgttcgattc ggtcttgtct ggtttgccat gttgtcttcc gcgtatgttg
     5281 tgggacaaaa agcacattta gccgtaacgg ttttaagtga aaaattaact ggcaaaaaaa
     5341 gtaaaatttt agaagttgtt gttcaaagtt tatttctggt atttgctgcg atgatcatgg
     5401 tgtatggcgg atggaatgct gtcacattga caatggggca gatctcgcct tctttaagta
     5461 ttcctatggg atatgtttat ctgtcagtac ctgtatcggg ggtactcatc attatctata
     5521 gcctgatcaa tctaatcgat acattaagca acaagccagc aattcaagaa aaaatataaa
     5581 aatggaggaa taaaatggca atagaagccg gattagtgtt attttttgta tttgttgtgt
     5641 tgcttgtatt agggatgccg attgctataa gtattgcatt ctcatcgatg atgactttat
     5701 tgttagttat accgtttgat gtttctgctt ttagttcagc tcaaaaaatg gtgggaagta
     5761 tcaatagttt ttcgctgatc gctattccgt ttttcgttct ctcgggcatt atcatgaata
     5821 atggagggat agctaagaaa ctagtggatt ttgcgatgtt gttcgtcggg cgcgtgccgg
     5881 gggctttggc tcatacgaat gtgttaggga atgctttatt tggttccatc tctagctcgg
     5941 ccattgccgc ttctaccgcg attggcggag tgttgatccc tcagcaagtg gaagaaggat
     6001 atgaccggaa gtttgcgaca gcggtcaata tcgcgtcagc tccgacaggt atggtcattc
     6061 ctccaagtac tgcttttatc atgtattcac ttgttgctgg tggtgcttcg atctctgctt
     6121 tgtttatggg aggttatctc gtaggcgctt tgtgggcgct aggaatcatg ctagtagcat
     6181 tcatcgttgc taaaaagaat aaataccctg ttgtcgaaaa aggacagatg gctcatgctg
     6241 gaaaaataat aaaagaggcg attcctagcc ttctattgat tgtcatcatt atcggtggta
     6301 tcttgacggg gatatttaca gcagtagaag cttcagcgat agctgtcatt tactccctgt
     6361 tgattgctat gttttactat aaaacggtaa aactaaaaga tatacctaat atgttgtttc
     6421 aatcagtagc aatgtcaggg acgatcatgt tcttattggc tacttcttcc atgatgtctt
     6481 tcgccatggc gtttaccggt attcctcagg caattagttc gttgattatg ggcgtaacgg
     6541 ataataaatt tattattctg ttattagtca atcttgtgtt attactggtg ggtatgttta
     6601 tggatgtggc gcctgctatc ttgattttta caccaatatt tttaccaatc gtcacaagtg
     6661 taggggttga tcctgtacat tacgggttgt tttcaatcat gaatctgtgt gtaggctcca
     6721 tcacgccgcc agtaggtaca ggactatatg tcggtgcaag tgtaggaggc gtaaaagcag
     6781 aggcaatgct gaaaccgctt cttccttttt atggggttat cttagtcgtc ttgtttttga
     6841 ttacttactt cccgcaaatc gtcatgtggc tacccaatat gctgagttag gagaatagtt
     6901 atgttgaaca ttacaattag aggacatgat ctttccgaag tccaaacgat agaagatctt
     6961 gcagaaaaaa caaaagaaca agggattcat acactgcagc tcgccttagg tctttcgttt
     7021 cctgaaatac cttctggcgc aaatgagatc aattctggga tgggaaatta tgtcaaacga
     7081 gttttagaaa agcaagaagt gaccgtagga attttaagtt gctatatcaa tatgattcat
     7141 ccagatttaa cgatacgaca agagttgctg acaaagttcg agcagtatct tcgctatgct
     7201 tcttcctttg gtgcgtcgat ggttgcttcg gaaactggat gtgttttgcc ggaaatccaa
     7261 tatacggaag aaaatttcac agatgaagca tttgctgaag ctgtgtccgt tatccgtcgc
     7321 ttggtaaaag ctggagaaaa ataccaaatg atggtgggga tagaaccagg tttgaatcat
     7381 ccggtgtatt ctttagcaag ggtagagcag ctgatccaag cagtagattc ggactatcta
     7441 ggaatcattt tagatccgac caatttgatc acaagtagtg attatcaaga acaagtccaa
     7501 ttggtagaag aagcttttga acgttttggt gaaaagatat gtgcggttca tttgaaagat
     7561 tttcgtgtcg aacaggaaaa aatcgtgccg gtaaatttgg gagacggtgt gatcgagtac
     7621 acaaaaatca aagaaatcat catgaagaat cgtccgtacc tgtatgtggt tcttgaagaa
     7681 acaaaagatg atgggattcg ttatggaaga agtcttcttg aataatgtaa agaagacgtt
     7741 ggttaactaa tatttagaat ggagggggcg gggctagaag atgccacgtc tcttcttttt
     7801 tataagagta tataaaagca gctctttttg attacaaaaa acgtggtata ataaaattgt
     7861 gcataagtga acaaaaggag gtgcggcaca tgctccaaaa gaaaatcagc ggtgaacaag
     7921 tcaatacgat ggtttctgat cagtatgaaa tctatcatgt caaagatgtc gtcagccaaa
     7981 ataaaacgat ctatcatcat catgatttct atgagatcca tgctacatta aagggagaag
     8041 cgacttttta tttgaacgga cgacaattta cgatcaaagc aggaagtgtg ctgctcatcc
     8101 attccaatga tttgcatcgt atcgtccgcc aatcttcaga tgtattcgag cgtgtctata
     8161 tctttgtcac cccttctttt ctagaaagtc gttccacgcc atggtctaat ctatcggctt
     8221 gttttcaacc aatgggagag aagaggagtc gtgtattgca aatagaacag gaagtattga
     8281 agcaaaaatt atcatttatc gaccatcctc ctgataaaaa aatttacggt gaagacataa
     8341 aatatgaaca agctctggta gaatacttga tttttctcaa tcaagttgta caaaaagaag
     8401 agagtgcgca agaacagaat gctgtagttc ctaatgagcg tgtggaacaa atgttacaat
     8461 atatctcaaa aaatttatct catccgcttt ctttagagga aatggaaaag cagttttttg
     8521 tcagcaaata ccatatcaca cgagaattta aaaagtacac tggatttacg tttcatcagt
     8581 atgtaatgaa gaaaaagctg atctatgcca agcacttgtt gcgagaatac cgcagctcta
     8641 gtgctgtata tagtaaaagt ggtttttctt cttatccgca ttttctgaaa gcattcaaga
     8701 gagagttcgg cataacgcct aaagaatttt tgaaacgtga tacacagaaa cagttggttc
     8761 attatgaaca ttttgaagat gaacaatgag aatgatcact aaaaaataac cgcagtagct
     8821 gctgttgttt acatagaagc agaaactgcg gttattttat ttttatctat atatttcaaa
     8881 atattttgaa aaaatgacgt acatattata tgaatcagct gaatttacga aagtctctcg
     8941 gcgatagtcc tgtttttgct ttgaacaggc ggctaaagta agcaaaattc tcaatcccta
     9001 tttcttcgga aatcattcga accgtaaatt ctgtataccg aagaaggtct ttagcgtaag
     9061 aaagtttttt gctgatcaaa taatcgattg gtggagaacc gataaatttg gaaaaatctt
     9121 tgctcagctg atatttattc aataagaatt gttgttctaa ttgatcaagt gtaatcgttt
     9181 ctttgaaatg ttcgtctaag tattgcttca gtttgtaaac gtaggcggga atatcctctt
     9241 cttcgaaatc tagctgatac ttttgcaaaa tcagttcatt cagcaactcg tggatagcta
     9301 cagaggactg gaaatcggta cgggcattcg tgccttgttg catgtggatc aattctgtga
     9361 tcaaatggtg gatcttgttt tctgatggat ctcctgttga atggaaggtg ttatggccgt
     9421 cttttgtgaa ctcctgataa aatgattgga cgttctgacc attcaaatgg atccaatcca
     9481 tttcccatgg ttcttcgctg atcgtttgat aacgctgata ttcttggcag tcaataaaaa
     9541 ataagtctcc agcttttagc tgttgctgat tatcattata tgtaagcgaa cctttgccag
     9601 acaaagtaaa ctttatcaag taagaaggta aatgtgctcg ttcagtatag taagggattg
     9661 gtgctttgaa gtaaccgatt tcttggatat aaaaaaataa attcctgctt ctttgtgtag
     9721 gcgtcaagat cactcttttt gagtcttccg accatgagtg gttggctttt tcccagtaat
     9781 ccatgtaaac actcccaact tgtcaagata atgcaattat ataccaaaat agtgtgaagc
     9841 aaaatataag attctgtatt atgatagcat tgtcaataaa tgataacgct ttattaataa
     9901 agtgctaatc aacaataaag taaaagaggt ggagaaatga atccaacatt tgttgccatc
     9961 gatatcggtg catcaagcgg acgactaatg aaaagtacga gggatgcaaa aggttctttg
    10021 atcttggaag aagtacaccg gttcaaaaac ggctttcgga acaaagacgg tcacgatcgc
    10081 tggaagatcg attatcttat caaagagatt ttgattggat ttgaaaaatt aaagcagaca
    10141 gggattgatc gcctttttgc ggggatcgat acatgggcag tcgattactg tctgattgat
    10201 gaacaagggg atcggataaa tgatccgatt gcttatcggg acaagcgtac caatggagca
    10261 gtagaacagt tttctgaaat ctattctcta gataagttgt atgagcagac agggattcag
    10321 atccagcctt tcaatacctt gttccaattg tttgctgaag aaaaagaaaa tttggcaaaa
    10381 gcacataaat tgttactgat acctgattat ttagggtatg tctttactgg taacatggtg
    10441 acggagaaaa ctaatgcatc tactatgcag ctgctgaatg cagggacaaa agaatgggaa
    10501 atagaacttc ttgaaaaaat cggtgtctcg aaggaattat ttccaccatt gacagagccg
    10561 ggaacgatac taggagatct gcaaaaagaa caattttcaa aatatgattt gccagatgtg
    10621 acctttatca cagtagcaag tcatgatacg gcgtcagctg tattaggaac gccaggtcaa
    10681 ggagaacgtt ggggttatat cagcagtggg acatggtcgc ttttagggat tgaaacaaag
    10741 atcgccaatg tttctcgcaa tgctttttct gaaaattata cgaatgaatg gggtgcccat
    10801 aacacggtgc gttttttgaa aaacatcatg gggatgtggt tgatccagga agttgcccga
    10861 atgcaagagt acgagtacag ttatgcggaa ttggcagagc ttgctgcaaa agaaaaaccg
    10921 ctgcagcaag tgatggaaat caatgaccct cgatttttga atcccccaaa tatgatagaa
    10981 gagattcagc aatactgttt tgaaacagag cagccagtcc ctgtctcatc gggagctatc
    11041 gctcgttgtg tgtatgataa tcttgctctt tgttacgcac aagagctgat caaagtagaa
    11101 cacttaactg gaacgaaatt ggaaaagctg cacatcgtag ggggcggatc gaataatcgt
    11161 ctactgaatc aattgacagc agattatgcc ggaatcattg tagaagcagg acctagtgaa
    11221 gccacagcaa tcgggaatgt tgccatgcaa atgctcagta caggtcagtt ttcgaatata
    11281 gaacaagcaa gaaatgtaat caaacagtcg tttccatgtg aaacttttta cccagatgaa
    11341 aataagcaaa acacgatgga atggtacgtg aataaaatca aaggagtaat cgtatgaaca
    11401 cgattgaaag aaattacgaa caagcaaaag aaaaatatgc aactatcggt gtagatacag
    11461 atgctgtact agaaaagata caggacatca aaatttctat gcattgctgg caaggagatg
    11521 atgtcaaagg tttcttgaca ccggatggcg aattgaccgg tggaatcatg gcgacaggga
    11581 attttccagg agccgcacga acacctgaag agcttagaca agatctggag aaggcttatt
    11641 cgttgatacc gggcaaacac aaattgaacc ttcatgctat ctatttggat acagaagaat
    11701 ctgtcgatct aaatgaaatt gagccgaaac attttgaaaa atgggtagag tgggcaaaaa
    11761 aagaagaaat cggtcttgac ttcaatccga ctttcttttc gcatccaatg atgaaagacg
    11821 gctttacatt ggctcatcca gataaagaag ttcgtgaata ctggatcgaa cacgggaaaa
    11881 gaagccgcaa aatcgcggag tactttggga aagaaactgg gcaaacaagt atcaataact
    11941 tttgggtacc tgatgggatg aaagataatc cgattgatcg ttttacccca cgggaacgtt
    12001 taatggaatc actcgatgaa atttttacag aagagttaga tgaacggtat gtacaagatg
    12061 cagtagaaag caaactattt ggtttaggcg ctgaagctta tacagtcggt tcccatgaat
    12121 tttatatggg ttacgggttg actcgtgaca aacttgtttg tctagatgct ggacattttc
    12181 atccaacaga agtgatttcc aataaattat cttcattagc tttattcagc aaaggaatca
    12241 tgttgcatgt ttcccgtcca gtgcgttggg attcagacca tgtggttttg atggatgatg
    12301 aattgcagga aatcgctaaa gaattagtcc gtaacgaatt gttggaaaaa acaaatatcg
    12361 gtctggattt ctttgatgca acgatcaacc gtatcgctgc ttgggtcatc ggtacgagaa
    12421 atacacaaaa agcactgctg aaagctatgc tggaaccagt tgagcgattg aaagaaatgg
    12481 agctggcttt tgactttact tcaagaatgg cttatacgga agaattgaaa gatttcccgt
    12541 atgccgatgt atggaattat ttctgttaca aaaatcaagt tccagttggt ttggattggc
    12601 tggaagaagt ccaggaatat caaaaagacg tgttggaatt aagaaaatag gagtgtggct
    12661 atattgaaaa aaatagatat tttacaagcg ccttatgtga aagaaatgat cgaaacgacg
    12721 tcgaatcttt atcgcttggg atgggatgaa agaaatggcg ggaatatttc ttatttacta
    12781 aaagaagaag agatcacacc gttcttggat cctgaaaaag tgatccgccg catctcgatg
    12841 atttttgatg ccagccgttt agcaggatgc tattttattg taactggttc aggaagatat
    12901 tttaaaaatg tagcgagcga acctgccgag aatcttggaa tcgttcgagt tgctgaagat
    12961 ggcaaaacac tggaactttt gtgggggctg gaaaacgaat gccttcctac aagcgagttg
    13021 ccaagtcatt tcatgagtca tatcgcccgt ttagaagtag acccagataa tcggatcgtt
    13081 atgcattgcc atgctagtca tctattagcg atgagtttta cgcatgaatt agacgaacgt
    13141 tcctttagtc gtacgctatg gcaaatgtgt acggaatgct tagttgtttt tccagaagga
    13201 atttccatca ttccgtggat ggttccggga acaaatgaga ttggggaagc aacagctgaa
    13261 aaaatgaaag aaactcgatt ggttttatgg ccacaacatg gaatctacgg gtcaggaaaa
    13321 gacatggatg aagtctttgg tttgatcgag acagcagaaa aggcagcaga agtctatacg
    13381 tacgtcaaag cgcaaggacc aatcttgcaa acaatcacgg acgaaaacct gtggcattta
    13441 gcagatgcgt ttggtgtcac tccaaaagca ggttatttgg aggaagttca tacgaaagca
    13501 ggtgtctgac atggtgaaaa aagctttttg catgaaagtt tacccagaaa aacaagaaga
    13561 atacaaaaaa agacatgatg aactttggcc agaaatgcgt acagcacttc gtgaacacgg
    13621 ggtcatcgac tattcgatct atcttgatcc gagtacaagt ttgttatttg gttacttagt
    13681 gatcgaagat gaaaagttat gggaaaaaat ggcaaataca tctatcaatc aaaaatggtg
    13741 gaagtacatg gaagatatca tggagacaaa tccagatgcc tctccagtca gttgggaatt
    13801 gatgccagtc tttgaactgt agcaacaagc aactgggcaa ccggttgctt ggggttcttt
    13861 cgtttttatt gtgaaaaata atacaaatta ggaggaatag aaaatgagta atcgaatgat
    13921 tttaaatgaa acatcgtatt ttggaaaagg agcgattgag tcgatcgtcc ctgaatttca
    13981 gcagcgagga ttcggcaaag cagctgtcat tactgacaaa ggattgatcg agcacggtat
    14041 agcaacaaaa gtaacagaac ttctagataa agcagcaatc gcttatgctt tatacgacgg
    14101 gatcgtacct aatccaacga tccaaaatgt aaaagatgga gtggcttttg ttaaagaggc
    14161 agaggcagac tgtttgatcg caatcggtgg aggttcgcca atcgatacgg caaaagccat
    14221 tgggattatt ttgacgaatc cagagttttc agacgtcatt agtttagaag gtgttgctga
    14281 tacgaaaaat ccatgtttac ctatattagc tataccaacg acttctggaa cagcagcaga
    14341 agtaacgatc aactacgtca tcacagatga agtaaatcac cgaaaatttg tttgtgttga
    14401 tcctcatgat atcccaatcg tcgcgttcat cgacagcgat atgatgatgg gaatgccgaa
    14461 aaaattggct gcatcaacag gtatggatgc tatgacgcat gctattgaag ggtttatcac
    14521 aaaaggcgct tgggaaatga ctgatatgct gcatttaaaa gcaatcgaaa tcatcggcca
    14581 ttctttggaa gcttctgtag atggcgatca aaatgggcga gaaaaaatgg cattaggaca
    14641 gtatattgcg ggaatgggct tctcaaatgt cggtttagga ctggtacacg ggatggctca
    14701 cccgttaagt gcatggtaca acattcctca tggcgtagcg tgtgcagcac ttttgccaac
    14761 tgtcatgaaa tataacaaag aatatactgg agaaaagtac cgtgagatcg ctcttgttct
    14821 agggattaaa ggagcagctg aaatgtcttt agaagatgta agagaagcgg cttgtggtga
    14881 gatcgatcgg ttgagcaaag cggttggaat cccagaaacc atttctgaat taggtgtaaa
    14941 agaagcagat attccagcaa ttgcagaaga tgctttgcga gatgtatgca caccaggaaa
    15001 tccaagagaa acgacagtag aagaaatcat tgctttatat caaagtttga tgtaaaaacg
    15061 aagcggatct tcaacattaa attgcctgta aagagaaatg gtagtttata tctggatatc
    15121 tcttttaaat tgatggtatt tccgctataa tttttcttat aaagaaatag gagaatatta
    15181 tatggaaaat aaaaagcctt atggaactgt attgatcaaa gcctctaaaa tactagacta
    15241 tttagcggaa aaccaagatg tctctttgca gacgatcgca aagggtgtgg gaatgacctc
    15301 ttctacagtc ttgaaaatac tagatacttt gttgatgatc ggctacgtaa ataaaaattc
    15361 agagaaaaac tatcgtttag gtgcaaagct tattcgctac gcgaataaaa acattgaaca
    15421 aatcgatttg gtagagttga ctttaccgtt tttagaacag ctgcagcaaa agatcgatga
    15481 gactatccac ttaggggtat tggacaacaa tgagatcttg tacgtgaaca aactagatcc
    15541 aaaacatcaa acgatccgca tgtcttcaaa aatcggaatc acccgaccat tatacaattc
    15601 tgcgatgggc aaagccgtat tagcagaatt cagtgaagag cagtacactg attatcttga
    15661 aaagcatccg ttgatccctt acacagaata tacgatcacc aatgcattac ggttgaaaaa
    15721 ggaaatcaaa acggtaaaag aaacccacat cgcatttgac gatgaagaaa tcgaacgtga
    15781 tattttttgt ataggagcct caatcgtgaa aaacgaacaa atcattggtg catttagtgt
    15841 aagtatgccg aaatatcgtt taacagatga gacaaaagaa caaataatca gtgccctcaa
    15901 gcaaacaaaa agcgaaatcg agcaaagaat tcaaaaataa tgaaaaaagc gtttgcaatt
    15961 tttcttttct gccgtataat atgggtatag atttccaagt tataaaataa aatttctaaa
    16021 taggaaatag gtggagaaga tgaaaaaaat tgatatttta tctcgattga aaaacgccgg
    16081 agtgattgcg gtggtacgcg gaaaaagcaa agaagaagct ttgaatgctt gccacgcaat
    16141 catcaaaggt ggtctaactg gtattgagtt gacttttaca gtccctcaag cagatcaagt
    16201 aatcaaagaa cttttgtcat tctacaaaga ccaaccagaa atagtgattg gcgcaggcac
    16261 agtattagac gctgtcacag ctcgcttggc tatcttagct ggcgcagagt atattgttag
    16321 cccttccttt gatcaagaaa cggcagagat gtgcaatctg tatcaaattc cttacttacc
    16381 aggatgtatg actattaccg agatcaaaac agcgctgaaa agcggggtag atatcgtcaa
    16441 actatttcct ggaagcgctt atggaccaag tatcatttct gccttcaagg cgccgatgcc
    16501 tcaagtcaat atcatgccaa ctggcggtgt gagtctggac aatatgaaag agtggttcga
    16561 tgctggtgtc gtaacagtcg gagtaggtgg gaacctttta gcacccgcag ctacaggaga
    16621 ttttgacaaa gtaacggaag tcgcgcaaca gtatgcagca aaaatgaaag aaatcaagag
    16681 gtagctgctt atgggaaaag tagtgacact cggagaaatc atgttgcgat tgtccacaga
    16741 gcagggaacc cgtatcgatt caagtgaacg attcgctgcc cattatggcg gaggggaagc
    16801 aaacgtagca atttctttag ctaattacgg acatgaagcg atttttgcta caaaagtacc
    16861 tgataacgca ttgggaaaag cagtcaaaaa gcacttgcaa tgttatggag tagatacgaa
    16921 acaggtatta tctggtggat caagactagg tacttattat atggaaactg gtagtggcga
    16981 acgcgccgcc tcagtgatct atgatcgtgc cggatcaagt tttgctgaaa tggatcattt
    17041 ggaatggtcg ctagaggagt tattccaagg aatagatata tttcatgttt caggaatcac
    17101 cccagctttg tctaaaaaat ggcaaaaaat gacgaaacag gtgatagaag ctgcaaaaaa
    17161 agctggctgc ctgatcagct tcgatatcaa ctatcgtgga aaattatgga cacagcaaga
    17221 agccagcgaa gtaatccatc agcttttacc tttagtagat atctgctcag ctggaaaaat
    17281 ggatgctctt tatttgttag gtatcgagaa agcgccagaa gcagaagaac aacctctaat
    17341 ttattattat caaaaaatgc aggaaaagtt cccgaatatt caggtgtttt attcgaccaa
    17401 gcgtaccgtt cattcagcaa gtgaaaatga attgaagggg acattgtgga tgaaccatga
    17461 atatgtggaa tcccaagtcc atagaatcac cccaatcgtg gatcgagtag gtggcggaga
    17521 tgcttttgca ggtggcgtgc tccatgggat tttatccaat atgtctccgc aagaaatcat
    17581 tgattttgct acggcagctt ctgccttgaa gcatacgatc catggggact gcaaccaatt
    17641 tagtcaagaa gaagtcgcac aatttttagc atgcggctct ggaaaaatca ttcgatagaa
    17701 ggagtagaag aaaatgcaag aaatggaaac acgttataca cacagtccag aagatatccg
    17761 tcattattca acggaacaat tgagaaaaga atttttagta gaaaaggtat ttgaaccagg
    17821 gaaaatcagt ttgacttata cgcacaacga tcgaatgatc tttggtggag tgacacctac
    17881 tgaaaaagaa ttagaaatca cattagacaa agaactaggt gttgattact tcttagagcg
    17941 tcgtgaacta ggtgtgatca atatcggtgg accaggattc atcgagatcg atgggcaaaa
    18001 agaagaaatg aaaaaacaag acggatacta tatcggaaaa gaaacacgac atgttgtatt
    18061 ttcttctcaa gatgctgaaa acccagctaa attctatatt agttctgttc cagctcatca
    18121 taaatatcca
//
LOCUS       DAYYME010000050        17964 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000050 DAYYME010000000
VERSION     DAYYME010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.050"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..425
                     /locus_tag="ACHBFW_001413"
     CDS             <1..425
                     /locus_tag="ACHBFW_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317725.1"
                     /translation="GMIEEMIYFYQLEAENLSNYGKLFEQAFYLTENGHLLSASMKKF
                     LGMMFHFVWNLQAYPFDFQLKNFFVWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKK
                     ILVLEKIGIIDGYQHPNEKELLRTMTVFQQIVSEIRGN"
     gene            427..1686
                     /locus_tag="ACHBFW_001414"
     CDS             427..1686
                     /locus_tag="ACHBFW_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM9317726.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            1692..2273
                     /locus_tag="ACHBFW_001415"
     CDS             1692..2273
                     /locus_tag="ACHBFW_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFM9317727.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(2355..3230)
                     /locus_tag="ACHBFW_001416"
     CDS             complement(2355..3230)
                     /locus_tag="ACHBFW_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9317728.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            3378..3710
                     /locus_tag="ACHBFW_001417"
     CDS             3378..3710
                     /locus_tag="ACHBFW_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM9317729.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            3822..5030
                     /locus_tag="ACHBFW_001418"
     CDS             3822..5030
                     /locus_tag="ACHBFW_001418"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFM9317730.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            5184..5639
                     /locus_tag="ACHBFW_001419"
     CDS             5184..5639
                     /locus_tag="ACHBFW_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317731.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            6001..6276
                     /locus_tag="ACHBFW_001420"
     CDS             6001..6276
                     /locus_tag="ACHBFW_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317732.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            6289..8367
                     /locus_tag="ACHBFW_001421"
     CDS             6289..8367
                     /locus_tag="ACHBFW_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM9317733.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            8530..10413
                     /locus_tag="ACHBFW_001422"
     CDS             8530..10413
                     /locus_tag="ACHBFW_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9317734.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            10425..11372
                     /locus_tag="ACHBFW_001423"
     CDS             10425..11372
                     /locus_tag="ACHBFW_001423"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFM9317735.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            11392..11919
                     /locus_tag="ACHBFW_001424"
     CDS             11392..11919
                     /locus_tag="ACHBFW_001424"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM9317736.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(11982..12635)
                     /locus_tag="ACHBFW_001425"
     CDS             complement(11982..12635)
                     /locus_tag="ACHBFW_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFM9317737.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            12767..13609
                     /locus_tag="ACHBFW_001426"
     CDS             12767..13609
                     /locus_tag="ACHBFW_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9317738.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            13767..14663
                     /locus_tag="ACHBFW_001427"
     CDS             13767..14663
                     /locus_tag="ACHBFW_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM9317739.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            14666..15379
                     /locus_tag="ACHBFW_001428"
     CDS             14666..15379
                     /locus_tag="ACHBFW_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFM9317740.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            15399..15917
                     /gene="msrA"
                     /locus_tag="ACHBFW_001429"
     CDS             15399..15917
                     /gene="msrA"
                     /locus_tag="ACHBFW_001429"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM9317741.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            15914..16141
                     /locus_tag="ACHBFW_001430"
     CDS             15914..16141
                     /locus_tag="ACHBFW_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFM9317742.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            16293..16844
                     /gene="lepB"
                     /locus_tag="ACHBFW_001431"
     CDS             16293..16844
                     /gene="lepB"
                     /locus_tag="ACHBFW_001431"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM9317743.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(16927..17469)
                     /locus_tag="ACHBFW_001432"
     CDS             complement(16927..17469)
                     /locus_tag="ACHBFW_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM9317744.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(17706..>17964)
                     /locus_tag="ACHBFW_001433"
     CDS             complement(17706..>17964)
                     /locus_tag="ACHBFW_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HFM9317745.1"
                     /translation="IMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQ
                     IAKENGGTFHEVDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT         6248 a         3046 c         3660 g         5010 t
ORIGIN      
        1 ccgggatgat cgaagaaatg atctattttt atcaattaga ggcagaaaac ctgtcaaatt
       61 atggtaaact gtttgaacaa gctttttatc tgaccgaaaa tggccatcta ctttctgcta
      121 gtatgaaaaa atttttagga atgatgtttc atttcgtgtg gaatctccaa gcatatcctt
      181 ttgatttcca actaaaaaac ttttttgtgt ggctggactt gcttggctat ccaatccact
      241 ggctggaaca gatttcaaag atggagtatt tggaaaaagt tctttttttg gcagaaaaaa
      301 gcaaaaaaat acttgtttta gaaaaaatag gtataataga cggataccaa catccaaacg
      361 aaaaagaatt attgagaaca atgacggtat ttcaacaaat cgtcagtgag ataagaggga
      421 attgaaatga atacaaatag cgaaaaaatg ttgcaagctt tatcagatga agacttgtca
      481 caagcacaaa tctttttaga aaaagcatta aaagaagacc cagcagatat attagctgaa
      541 ctaggcgaag aattgctggc tattggcttt ttgatcgaag caaaacagat ttttgaacaa
      601 ctgatcaacc aatacccaga aaatgacgga ctgaacatcc cgttagcaga aattgcaatc
      661 gaagataatg agatcgactt agcttttgaa tacctggaaa aagtggataa gcaaagtgat
      721 tactatccgc aaagcttatt ggcgatagct gatttgtacc aagttatcgg aatccctgaa
      781 gtgagtgaag caaaattgaa agaagcagct gggttgcttc ctgatgaacc attgatccag
      841 tttgcgctag ctgaattgta tttttcagtt gatcgtttca aagaggcagc agtggtttat
      901 gaaacattgt tggcaagcgg gataaatgaa atttcaggaa tctcgatgca ggaacgttta
      961 ggacaatcct taagtatgca aggagaattc gaagcagcta tcgtaccttt agaagaagca
     1021 ctgaaagaag aaaggacaga tgatcgactg ttccagctag cttttacgaa tcttcaattg
     1081 aaagaaaatc agcaggcaat caactatctg gaagaacttc gagaagttaa tccgcattat
     1141 caatcgttat atctatatct aggacaagct cttcaagaag aagaaatgat tgaagaagca
     1201 caaacagtgt tagaagaagg aatcaaagaa aatccttacc aagtcgaact ttatcacctg
     1261 gcctcggaaa atgctttccg tctgcatgat agacagcgag cagaagaact tttactgaaa
     1321 gctttagaag taggagacaa acaagatgaa acattactta ctttaagtaa tctttacttg
     1381 gatgatgaaa gatatgaaga tgtgatacaa gtgatcaatc agatggaaga aactgctaat
     1441 ccttatgcag aatggaacct agcccatgct tataatgaat tagaagactt taccgttgca
     1501 gctgtacact atgaacaggc ttatcatgaa ctatcacatg aaccagattt cttgaaagag
     1561 tatgcctttt tcttaagaga agaagggcag ctgaaaagag cgcaagaatt attgacttat
     1621 tatttaacac ttgaaccagg agatatggag gcactgtcat tattagatga cctaacagag
     1681 aggtgagaaa aatgctaatt gatgtacggg aaaagaagaa ttttttaacg tggatggtca
     1741 atcatgtatc ttttagccga agagaagttt tttggatctt gaattatctt gccaatcatg
     1801 aagcgatttt aagcaatgtc cactttgttg aaggggcgaa ttttgcggat cgaggactgc
     1861 agatcagtga tctatctgtt gaaggggaac ccatgaaatt gtttttgcag ggaaaagaat
     1921 ttacggacac agaccagatt tttcatgaaa tccgtttgaa ttggaaaaag cctttatatg
     1981 tggaatgtat attcgaaaat gcttggcaga caagagaata tctatctatc ttagaagaca
     2041 atccattttc ttcttgggat tcttcagtca gtgaagaaga tactgaatta attgaacgat
     2101 attttcaaga aaaagaagaa gaggcaaaac taaaaatgct ttatgctcaa atcgatcaag
     2161 cactagaaga tggagatagg gatgcttttt tgaaattatc tgatgaagtt aaccgaaaga
     2221 tccttttgca gacacaaaag aaaagtaacc gggtaagtga acataacaat tagttaaaaa
     2281 tagtatagta ctaaaaaaga caaaggtcag ctggatgtgg aatctttcct catagcctat
     2341 ctttgtcttt tttcttatag ttttttcagt aatcttgtct gtggcttcaa ataagtaaag
     2401 ccttgccctt ccacttcatg cacattgatg attgaaagga aagctttttc gtcaatctcg
     2461 tggacgatcc tcttagcttc agttaattca ctagagctaa cgaccatata gatgattttt
     2521 tttgaattac cagagaatgc gccttctcca gaaaggaaag tcacacctct ttgcagaccg
     2581 gtcatcagta gtggtgcaat ctcttcgctt ttgttggaga cgacaagtag accttttgca
     2641 gaatatccgc catctaagat cgaatcaatc acacgactga aaacatacga aacaatcaac
     2701 gtatacatca tacgtttcaa gtcgatatag gttaatgaag caagtaagac gatgatatca
     2761 aatgccaata gggaacgccc catagtgatg ccgtaatttt gttctaaaat ccgagcgata
     2821 atatcactgc cgcctgttgt tccacccatg cggtagacga tcccgcttcc tacacctgca
     2881 actaaacccg caagtagaga aacgatcagt agatcatgct gtaaattgat ttctagtgga
     2941 accctttgcc agatccataa aaagaatgac aaagaaagtg tacctagaac agtataggcc
     3001 aaggaacgtt taccgaggat ctttcctcct agtaaaatca atgggatgtt gattaataaa
     3061 gtcgtataag ctggatcaat accaaataaa gctcttaaga tcaaggtgat ccctgagact
     3121 cccccttctg ctaaatgatt agcgatgttc aagtaaacaa gaccaaatgc ataaatactt
     3181 gttcctataa tgataaaaag aacttctttt actgtttttc gtttgaccat atgaattcca
     3241 gctttcaatt atgtattttc acagaatttc catttagaaa ttccttccta aatgtagcat
     3301 ggaaattcga ttttctcaat tgctcttatc taggatttgc gttaaagttt tggtaagata
     3361 gttttggagg gaaaaagatg gataaaaagc gttctttata cagtatgcag caggaagtgg
     3421 atgaatatat ccagcaattc aaggtggggt atttctctcc attagctcag atggctcgat
     3481 taactgaaga agtaggtgaa ttggcaagag aagtcaacca cacttatgga gaaaaaagca
     3541 aaaaggcttc tgaaccagtt aactcggtag cagaagaact tggtgatgtt ttattcgtta
     3601 cgatgatcat ggctaattca ttgaatatcg acttaactga agtttttgaa aaaaacatgg
     3661 aaaaattcaa tcaaagagac cataatcgct ttgagcgtaa ggacgagtaa acaagatgaa
     3721 gtggtcctgg ccttgaagac tctgaaacgg acttgtcaaa cagtcaccat gcaactgagt
     3781 ctttatctca ggacaagaaa cacgcaggaa aggttggaaa aatgaaatta aaacaattac
     3841 ctctggaata ccaaaaggca attccagttc tgaaaaaatt agagaatgca ggatatgaag
     3901 cttattttgt aggtggaagt gttcgtgata tcttgctgaa ccagccaatc catgatgtgg
     3961 atatcgcaac aagcgcattt ccagaagaaa tcaaacagct ttttccaaaa acgatagata
     4021 ttgggatcga acatggaact gtgttggtat tagaaaatga tgaacaatat gagatcacca
     4081 catttcggac ggaatcaact tatcaggact accgtagacc agatcatgtc gaattcgttc
     4141 gttcgttggc agaagatttg aaaagaagag attttacgat caatgcgttt gctttgaaag
     4201 aagatggaga aatcatcgat ttgttcgatg gtctgcaaga tctagaagaa cagatcttgc
     4261 gagctgtagg caatcctcat gagcggttcc atgaagatgc cttacgaatg atgcgcgggt
     4321 tgcgttttgt cagtcaacta gggttcaaat tagaggaaga aaccttctac gccatcaaag
     4381 aaaaccacga gctattaggt aaaatatctg tagaacgtat aaatgtggaa ttcatcaaga
     4441 tgctcttggg aaatgaacgg gccgcgggaa tccatgcatt tattgaaaca gagtgctaca
     4501 tttgctgtcc cggtttgaaa gaagaaggcg aagcactttt acgttttgct gaattaccat
     4561 ccgtcccatt gaaagaagag agccaagcat gggcattatt gatcagccaa ctggatctaa
     4621 aagaaaatga gattcgttcc tttttgaaat cgtggaagtg ttctaaccaa atgattaaag
     4681 acgttcaagc acttgtccaa ggactacgac aacgagaaac tggagctttg gaaccattcg
     4741 aattgtactt cttaggcaaa aaacatgcac tgcaagtaga agaattgatg atctattttg
     4801 acgaaacacc tcagatggat gccgtaggag aatcctatga tcgtttacct atcaagtcat
     4861 taagcgaatt agcagtagat ggtaaaatct tgctagaaga aactggaaaa aaacctggta
     4921 agtgggttag tgaagctttg caattagctg aaaaagccgt cgtagaacaa agaataaaaa
     4981 atgataaaaa acaggttttg gattttttat cgaaagaaaa attgatttaa aaaacactag
     5041 actgtatctg ctcagctttg ttggctactt aaagtgggca gatacgtcat tagaagaaaa
     5101 aacataagat gtgaccactt acataaattt gtgctaaact gaggtagtac aagaataaat
     5161 tacatcataa aggagtcttt ttaatgagta aagaaatgac agcgctaaaa ttttatttta
     5221 gaaatggaga aacctggacg atcaaccgcc gtcatatcgg cgatctttgg atcaaacaaa
     5281 tcacaacaag ttttggacga atcaatggca gtgaatttgt tgaaatccat ccgtgtgctg
     5341 gatttaaaat cgaaatcttc catgaagggg acgcagttgc gacacatgat atcaacctag
     5401 gcggactgga aatgggcatg tttaatcgcg cattgaaata cgaagatatc gagcgaatgg
     5461 aaatccttta ccgaaatggc acacctgatc ttgtgtactt cccatatcta gataaaggaa
     5521 cagaaggatt agacaatcaa tatcaatcaa caaaaatcag cgaaaaaaca ggtaacttgt
     5581 atatcgtgat caatccagat caacgtgtag aagatgtata cggggaattt ttcgaataaa
     5641 tcgattttta cgcaaaaatg aggctgtgaa agagatgtgt cttattacta ctgataggct
     5701 gtctcttcca caccgtttat tcagaataag ggactgtgac aagatttttg tcgcagcccc
     5761 atttttttgt tgtgataaaa gaacaagaaa ggcaggaatg ggaaagtaaa catccttttg
     5821 tcagaaactt ttatctttct taagttttac ttgatatctg atactatttg gaatagaatg
     5881 aggcgttgaa aaatttatgg aaatttctaa ctagcaataa acgatgaaac aatgaaatat
     5941 ttttcatcca aaaagaactt tgctataatg atggaagttt gtgtatagga gatctggaaa
     6001 atgagagaga gttttgaaca acaaaaaaaa ctgttgcatg atcgctatgg cgccctttcg
     6061 atggatgacc gccgtcaaat actttgtaag ttgcgtaaaa gaaatatatt gatgtatcgt
     6121 cagttggaac gtctgaaaca tgatctgctt cgtttggagt caaaacgtgt acagtgtgaa
     6181 ttagagggga accaaacaca ggttgaagta gtcgaaacca aaattttaaa gaaaaaagaa
     6241 caatttttaa aaatgcttac acaaaataaa aaataaaggg gatggacgat ggaatttgct
     6301 gagttagtta ttgtatttgc agtaacgatc accttatcga acatagctag ccgcattttg
     6361 ccaatgattc cagctccact gatccaaatt ttccttggag tggtattagg tctgacagag
     6421 tggggacaat caatcgattt tgaacctgaa ttgttcttag ttatgattat tgcaccattg
     6481 ttatttagag aaggagaaaa agcagatatt tcttcgattc tcaaaaattt cgggacaatt
     6541 ttgttcttgg catttggagg agtcattttg acccttgtcg gggtaggtgc gacgttatca
     6601 ttcctgttgc caagtgtccc acttgctgcc tgctttgctt ttggagcagc gttaggtccg
     6661 acagatgcgg tagcagtcag ttctctttct ggacgagtga atatccctaa aaaggcgatg
     6721 catattttgg aaggagaagg tcttttgaat gatgcatctg gtgttactgc cttccagttt
     6781 gctcttggtg cgctgatcac cggatctttt tcagcagtaa acgcggggat gagtctagta
     6841 gtttctagta ttgggggagc tttgatcggc tttttgcttg tctggttcaa acaaaaaata
     6901 attcatttga tcgaaaaagc atcagcacaa gatgtcacag ggtatctgct gatcgaactt
     6961 ttgcttcctt ttctagccta tgtattggca gaggcatttg atgtatctgg aatcattgct
     7021 gcagtggcag caggtattct ccaagcttct ggttttagga aaatctctgt gtttgatgca
     7081 gaattgtcga gcttgtccca cagtacatgg acaactattg cttttacact gaatgcatta
     7141 gtatttatat ttttaggaat cgaattgacg caagtgttct cgccagtatg gggagacggt
     7201 ttatatccaa acggtctttt attagcgatt atcgtattaa tctctgtcat gctttttgtg
     7261 atcagattca tttctatcag tcttttttat gtattcaaag atggcagtaa aaagttcaaa
     7321 aaacagttga acgagatctt gatcttaaca tttggcggtg taaagggaac agtcagtttg
     7381 gctacgattt ttattttgcc attttcaatc aataatatga tgttttacca gcggtctttg
     7441 cttttgtttc tgacggcggg tgtcatctta gtgactttgg tcatagggat cattgtcttg
     7501 cctatgctga cggaaacaga ggaagcgaaa agcacggatt tgaatgcttt gatgatttta
     7561 gaagaggtcg ttgaagtttt gcgaaaagaa ataaaggaaa ttgatcgtaa tacaaaagaa
     7621 tttttagcca ctgaagcagt gatagaaaat tatcaagaac ggatacgtga tctgtacttg
     7681 gaagatttaa ccgatgatga aaagcaagaa gtacaagaaa tacaagcact gattttgtcg
     7741 atcgaacggg atggattgga tgaaagttat cgttcgggca agctttcttc taatggttat
     7801 cgtttttact cccgttttct ttctcgattc gaacattcta tcacaagcca gattttgtct
     7861 tttatcggct tttggttcat tgttgtccgt aggttaatgc gaatcgttct ccatccgaaa
     7921 atgttttggc aaagaagata tgctgatcgt cagacattta ttagtgatga agatatccaa
     7981 gaaatcagag agacatatca aaaaaatact cagttgatta ttgaaagttt agataacttg
     8041 actgatattt atgatgaaac gatggttcaa ttttttatcc agcaacgcaa acttgaagga
     8101 ataaaaatga actcggggaa tttgatttct tcgtggatga tccagcaaga tagtctgttt
     8161 acgaaaaaaa tgctaagagg ttattatctt gaaagaaaaa tcattgatga atacgaagtt
     8221 gctgaaaaaa tcacgacgtt ttctgcgaac agctatcgca ggaatatcaa tttgttagaa
     8281 tcttatacga tgaacaaacc atctgataac ttttctttcc gatttgcgtt tcgagcaaag
     8341 tcaaaaaaaa cgagtccaat aaaataaaaa ggtaaaaatt ttcaagtttt ataaaaaaca
     8401 aaacatatca gaacaggatc gaatcggtcc tgttcttttt ttgagaacac aaaaagaaca
     8461 aataccaaaa aaattttcct gaagttcact atttttatga tacaatatcc aagataagga
     8521 gggaacatgt tgaaagaatt aaaagtaact gacctcaaaa aaacttatgg tgaaaaagat
     8581 ttgtttgacc agatttcttt tttggtccac gaaaaagacc gtatcgggtt gattgggacg
     8641 aacgggacgg gtaaaacaag tctcttaaat attattgccg gtatcgatag cagcgacggt
     8701 gatcggcaaa cagtgtttta tccaactgat taccgtattg gttatctttc tcaagcagct
     8761 gaattttccg atgaattaac tgttctccaa gcagtctttc aaggaaacag ccccttgatc
     8821 caaactgttc gagcatatga ggaagcattg atcgaactag gggaaaacgg agaagaccct
     8881 gatgtgcaaa aacgttatgc taaagcagaa gaacaaatga ataaagaaga tgcttggact
     8941 acagatacca acgcaaagat tattttgcaa aaattaggaa tcagtgaatt agataaaaaa
     9001 atcagtactt tatcaggtgg tcagaaaaaa cgagttagtc tggcgcaagt gttgattgat
     9061 gaacccgatt tactattact agacgaacca acaaaccatt tagattatga agcgatcgag
     9121 tggttggaaa actatttgaa acaataccgt ggcgcactat tgatggttac ccacgatcgt
     9181 tattttctag atagagtagc aaaccgaatc ttcgaacttt catttggaaa attatacgag
     9241 tataaaggaa attatgaagc ttacgtctta gaaaaagcag aacgtgatcg tgtagaagta
     9301 gaacaagaag aaaagcgcaa gcggctttat aaacaggaat tagcttggat gagggctggc
     9361 gtacaggcac gtggcaccaa gcagcaagca cggatcaacc gtttcgaaga tctaaaggag
     9421 aacttgtacc aagtaaacca agaagatgat ttagagctga acctagcgac acaacgttta
     9481 gggaaaaaag tactggaaat caaagatggc agttaccgta taaatgacca aacactgcta
     9541 gaacatttag atttattgat tcaatctcgg gaaagactag gtattactgg taaaaatggt
     9601 gcgggaaaat cgacattgct taatattcta gctggcagaa ttcccctgga tagcggtaca
     9661 atgtctatcg gtgaaacggt tcatcttgcg tattatacac aagaaaatga agaaatggca
     9721 ccagataaac ggatgattgc ttacttacaa gaagctgctg aagaagcaaa aacagcagat
     9781 ggcagtcaaa tcggagtagc agaattactt gaacgatttt tatttccaag atttatgcat
     9841 gggacactga taggcaaact ttcaggcgga gaaaaacgcc ggttattttt gttgaaactg
     9901 ctgattcagc aacctaatgt tttattattg gatgaaccga ccaatgattt ggacatagca
     9961 acattgacga ttttggaaga ttattttaga agttttccag gtgctgtgat cactgtttcc
    10021 catgaccgtt atttcttgga caaggtagca gataagttat tagtattcca aggaaacgga
    10081 aaacaagaac tttattacgg aaacatgtcc agttatttac tgaagcaaaa agagacccag
    10141 cagcctgctg aaaaagcgaa accaaaaata cagagcaagg aacctgctga aaagaagaaa
    10201 ttatcttaca tggaacaaaa agaatgggaa acgattgaag atgagattgc agaacttgaa
    10261 gaaaaaatct cattgctcca agaagagatg aatcatcaag gagataattt cactcgatta
    10321 caagaattgc aaaacgacgt atctgaaaca gaagcacaac ttgaagaaaa aatggcacgg
    10381 tgggaatact tgagtgaatg ggtagaagat taggaggaaa aaggatggaa caagcgtatc
    10441 ttgacttagg aagaaaattg ttggaagaag ggcatcgtaa agaagacaga acaggcacag
    10501 gcacattaag cttattcgga taccaaatgc ggtttgatct gcaaaaaggg tttcctttgt
    10561 taacaacgaa acgagtacct tttggattga tcaaaagcga attgctttgg tttttaaaag
    10621 gagatacgaa tatccgctat ctattacaaa ataaaaacca tatttgggat gaatgggcat
    10681 ttgaacgcta catcaaaagt ccagactata atggaccaga tatgactgat tttggccata
    10741 gatgtttgac agatcctgaa ttcaatgaag aatataaaaa gcagagcaag cttttttgcg
    10801 agaagatcct aaccgatgat tcctttgctg aaacatacgg cgatcttgga cacatctacg
    10861 gctatcaatg gcgtcactgg gaaacaaaag acggtggctt tatcgatcaa atcaaacaag
    10921 tgatcgaagc aatcaaaaag actcctgatt ctagaagatt gatcgtttct gcatggaatc
    10981 cggaagatgt tcctagcatg gctttgcctc cttgtcatac gatgtttcaa ttttatgttc
    11041 aagagggacg gctaagctgc caattgtatc aacgcagtgg ggatgtgttt ctaggcgttc
    11101 cgttcaacat tgcaagttat gcgttgctga ctcacctaat tgcacatgaa acaggtttag
    11161 cggttggcga atttgttcac acattaggag atgctcatct ttatatcaat catatcgaac
    11221 aaatgaaaga acaattgagc cgagaaatac gcgcatttcc gactttagtt ttgaatcagg
    11281 aaaagcagtc agtatttgat tttgagatgg aagatatcca attagaagga tatgacccac
    11341 atccggcgat caaagctcct atcgctgttt agaatcagaa aggatgagaa aatgttagct
    11401 gcaatttggg cacaggatga aaacggattg attggaaaag aagaccgtct tccttggcgt
    11461 ttgccaaatg atctgaaatt cttcaaacaa atgacagaag caaatacact agtgatggga
    11521 cgaaaaacat tcgaagggat gggtagccgt ccgttgccga atcgtcagac gattgttttg
    11581 acgagagatt cttcctataa agcagaaggc gtgcatgtca tgcatagtgt ggaagacgtg
    11641 cttgctctgg aaaaagaaac agacggtatc tttttcatcg ctggagggtc agctgtttat
    11701 caagaatttc tgccattttg tacgatatta taccgtacag tcatccatca tgtattcgat
    11761 ggagatgctt attttccacc tgttgactgg tctgattggt cactgatcaa tactagtcaa
    11821 ggagaaatgg atgaaaaaaa tcgttatccg catcaatttg aaacgtatca gcgtaatgag
    11881 aatcctgtaa aaagcgggac aggcgaattg acgatataaa aaagctatgg atagactagc
    11941 tgttatttag agctgtccca tccatagctt tatttttttt attaagtaaa gaataaaaca
    12001 gagaagtaca taaaacctgc acctaacata acaaacaaat gccaaacaac atgcatatag
    12061 cggatagaat tcaagctata aaagaatgcg cctatcgtgt aagaaagccc gcctgctacc
    12121 atcaaaataa caccagtcat tccaagagag tgataaagag gaacagcagc cacgacacag
    12181 agccacccca taaaaatata gatgatcgta gatacttttg atactctctc ccgtttatgc
    12241 aacgtcaacg ctttatatat aatgccaaga atcgcaaaca accagattaa tacaaataaa
    12301 gtccaaccta gccatcctcg gatactcagc aaacaaaaag gagtataact tcctgcaatc
    12361 aaaagaaaaa tcgaactatg atcgaatact tggaacacct tttttgcttt tgtaaaaatc
    12421 aatgaatgaa acaaagtaga cgttaaaaat aaaagaatca tcatcgatcc atagatagca
    12481 taagagacaa tatggaggga ggaatgcaag tgggcacctt ttactaaaag aaccactagt
    12541 cccgcgatac tcaatccagt cccaattcca tgggtgactg cattaaacac ttcatttaat
    12601 atttcatacg ttcgattaaa tttcgttttt tccatgaaaa cggctccttt actaggtgta
    12661 attaatgaaa aatcattatt tgtctgttat actaaaaata gaaattgtct tacatgtagt
    12721 ttaacatgaa taaggcatct tgttaaagaa agagagagag aaaataatga caaatgtaaa
    12781 aatcgtaaca gattcttcat gtacgatgga aaaaaatgta cgcgatgctt taggcattcg
    12841 tgtcatgccg ctatccatca tgatcgacgg aacagtatat cccgatgatg accaactaga
    12901 cggcgaaaga tttatggaaa tgatggcgaa agcaaaaaat ctgcccaaaa caagccaacc
    12961 accaattgga gaattcatct cgatgtatga tgagctagga gaagacggca gtgaagtgat
    13021 ttcgatccat atgacaaaag gcttgagtgg aacagtagaa gcagcaagac aagccagtaa
    13081 tctaacaaaa acgaaagtaa cggttatcga cagtgatacc actgaccaag gattatcttt
    13141 tcaggtaatt cgagcagctg aattagcaaa agaaggtaaa acagtagaga aaatccttga
    13201 agaagtcaaa aaagtacaag acaatacaaa attatacatc ggaatatcca cactagataa
    13261 cttggtaaaa ggtggaagaa tcagccgaac aactggtttg ctgtctagtg ttttgaacat
    13321 acgcgtggtg atgaatttcg cccattctga attaatccct gtaactaagg ggcgaggaaa
    13381 gaaaacgttc gataaatggt ttaataattt gaaaaatgaa ctgaaagaat tgccaaatgt
    13441 gcgacaaatc ggtatttcac atgcggatgc aaaagaattg gcgcaagaat tcaaagaggg
    13501 tttgcaagaa ttattcccgg acatgcatat ccctgtactg catacgaatc cggttatcgc
    13561 tacgcatact ggtaaagacg cgtttgcaat catgtattat gtagattagt cgaaaagagg
    13621 ataagccctc agcctgaaag gcttgcagct tattctcttt ttctttataa aattaggaaa
    13681 ataattgttt tcttttttca taaagactac ctttttgata aaatttttga taacattaga
    13741 accaatcgaa tacaagaggt gaaaaaatga aattaacgaa acgacatttg atcctcgttt
    13801 tgttcccatt attaattgcg atcatcatct atgcaggttt agctgcttct ataccgaaag
    13861 ctggcccact aatgaaacca gtaactactg tgagagaaga tccaaataaa aaagaaaaac
    13921 ttcactacat agctatcgga gattcgctga ctgaaggagt aggagatcaa accaagcaag
    13981 gaggattcgt tccccttgtc gcaaatgatc taaaagaccg ttataatttg accgcggttg
    14041 aaacagaaaa ctatggtgta aatggtgaac gaagtgacca gatcttaaag cgagtaaaaa
    14101 caaaagaaga aattcgaaac aatatcaaaa cagctgattt tatcacactg actgttggcg
    14161 gtaatgattt gatgaaagtt atccaaaata atttattcgg gttaacggtc aattccttta
    14221 aaaagccact gaaaaactat caggaaaatg tcacagaact attggaagaa tttcgatccc
    14281 tcaataaaac cgcacctatt tacattttag gcatctacaa tccattttat ttgaactttc
    14341 ctgagatcac tgacatgcag acaattgtta acaattggaa caaaggaaca gaaacggtga
    14401 caaaaaaaat gccgaactgc tactttatcc caatcaatga tttgctttat caaggattga
    14461 atcaggaagt aggtgtcaca gaagaaggaa cagatcaaac aactagcacg acagaaaatc
    14521 taaatggcat caaaaataat gtgctctatg aagaagatca tttccatcca aataatttag
    14581 gttatcaatt gatggcaaat gcagtgagag atcagctgat cgagacacaa aaccaatggt
    14641 tagagaagga ggatgatcaa taaacatgaa aacaagatct gacgtatctt caaacaaaac
    14701 aaagaaaaca aattactgga aattcgcctt ccttatcctt cttggggtag tacttggttc
    14761 gaccgtattt ttaggcagcc gtatttttgc taatcgggaa ccagaactcc cagaaatacc
    14821 agctcttacg gaaagacaag gagatcctgt cttaacgatc aattcaaata aagaaaaagt
    14881 caatcaaatc atcagctttt tccttagtga gtatcaaaaa gatagtgata ttgaatataa
    14941 attttatttg gaaaatgaag cactactcaa tggtactttc gaagtactag gttttcctat
    15001 cgatttctat ttatattttg atccgtatgt gatggaaaac ggaaatgtcc aactaaaagc
    15061 tagaagtctt tctatcggaa cattgagttt gccaatcaaa gatgtgatga atatgatcaa
    15121 gagaaactat aaattaccag attggatcga agtcaataca gatgatctga ctatcatgct
    15181 taggttggat caattccgaa tgcaaaacgg gatgtatatt aaagcagata aaatcgactt
    15241 agtaaatgat gacattcggt ttagtctata tttgccaaaa gatacctctg atgcttcaaa
    15301 caactcaaat accaacaaaa ctagcaccag cagtactgaa agcaagacaa aagaaaataa
    15361 aaaatcatcg gatgagtagt attagagggg gaatcataat ggaacgagca atatttgcag
    15421 gaggctgctt ctggtgtatg atccagcctt tcgacacgct tcctggtatc catacagtga
    15481 tgtcaggata tactggaggg catgtaccag atccgactta tgaacaagta aaaagccaaa
    15541 cgactggaca tacagaagca gtggagatta tctatgatcc aacattgatt tcatatgaac
    15601 aattagtaga actttactgg cagcaaactg atccaactga cgctttcgga caattcgagg
    15661 atcgcggtga caattatcgc ccggtgatct tttatttgac agaagaacag cggatcattg
    15721 ctgaaaaaag taagaaacag ttagctgaaa gtggacgttt cgaacagcca atcgttacaa
    15781 aaatcgaacc agcccaacca ttttatctag cagaagaaga ccatcaagag tattataaaa
    15841 agcatcctga aaattttaaa agaaaccatg cacgccgggc tgcctttatc gaagaaaatt
    15901 ggaggaacga agggtgaatc gaagcttttt tcattattta atgacactgc gaggcggtgg
    15961 cacacatgat gctttatctg ttttcgctac agaagccggt aaagacatcc aatttcctaa
    16021 gcacagctct agctacgaag aaatttctaa ttatctggaa ctgaatgtcg actatttgcc
    16081 gtccatggat atctttgacg aagcatggga aaaatattta gaaaataatc atgacatata
    16141 agagatagtt ttgtacttta tgatcaaaaa agttttttgt ccaatagcct aattgaaaga
    16201 attattttta tttcagctat ctagtcgcat ttttttgata tttttcttat aataaagaaa
    16261 gaatgaaaaa aagagggatg gattgataga gattgacaaa aaaacaacgc tatattaatc
    16321 gcttctggct tgtattcaaa tacttacttg tttctgtatt ccttgcgttc atgttgcggg
    16381 gatttttatt catcccggta ccagtggaag gcaattcaat ggaaaacgtg ttgaagcaag
    16441 gtgatatggt tgtgatggaa aaattttcgg agatcagacg atttgatatt gttgtttttc
    16501 aattagcaga tggcacgatt tatatcaaac gagtaatcgg tcttccagga gaaaatgtca
    16561 gctaccaaaa tgatcaattg aagatcaatg ggaaagtagt taaggaacct tatctaacta
    16621 aaaacctgaa atcagatcat gcaaatgcat cctatacgac ggactttaca ctacaagagc
    16681 taacgggaca atccaagctt ccggaagata attattttgt acttggagat aatcgaagag
    16741 tttcaaaaga tagtcgttcg tttggcacga tcaataaaac tgatattcta ggtaaagcaa
    16801 gatttgtcta ttatccactt gatgaaataa aatggatcca gtgagaaaca ataaagcgtg
    16861 cagtctgttg agactgcacg ctttattgtt taatccaaaa tcagtttact accttagtac
    16921 gtaaaatcaa acagctcgtt taaacacttt tctagcctct aaagttttga taaatgaata
    16981 aacaaccatt atttgaatag ggatttggat catattcctg atgaatctac ctagaaaaat
    17041 tactttgaaa tctgcaccaa acaaaacaga catcagccaa gaattgataa aaaggctgac
    17101 tgggatcata atcaataatt gggcagcgat gacggaaggc aggtttatct ttttatgata
    17161 taaaaataag ccattaataa atccaccaag gaaagccgtg ataagaaaag cagcttgaaa
    17221 cgttcctgaa ccaaatatcg tgaaatttaa agtgttcgac aacatttcaa gcaaaccagc
    17281 ccagatcggt ccatatagat aacccataag cgccatactt aaaaatccga atgtgatttg
    17341 aaaataagca ggaccaatcg ctatcttgta taagatgaca tcaagggcta ataaaagagc
    17401 agaggtcgac aacaatcttg cattcatttc gggcaatcta aaaacgtctt ttgcaatcaa
    17461 agtgttcaaa atattctctc ctttgtcagt taataataaa aggataccat aaaatcacca
    17521 atgtgtcatg acacattggt gatttacata cactggcatc taacaattaa aaaaagggtg
    17581 agaagactgc ttttataaac tgtaaaaaag agattgtacg aataaaaaaa atagaggcgg
    17641 agaatactta tcgtatcatc cgcctttgtt tttttgttat aaaaaacgtc agattagtta
    17701 tctgcttaca tcttcatcca tacgggttct aatgtatttc cgtctagatc ttggacttct
    17761 aatgaaaaca tttcttcttc aggcattccc atatctactt cgtggaaagt accaccattt
    17821 tcttttgcaa tttgagcaaa tttttttact tcatccgcat tttcaacact aaacgaaatc
    17881 aaagcaccac ttgttttttt attatcagct atttctttat cttttataaa ttttttataa
    17941 aaatcatgag agagaagcat tatc
//
LOCUS       DAYYME010000051        17878 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000051 DAYYME010000000
VERSION     DAYYME010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.051"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(8..1591)
                     /gene="abc-f"
                     /locus_tag="ACHBFW_001434"
     CDS             complement(8..1591)
                     /gene="abc-f"
                     /locus_tag="ACHBFW_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFM9317746.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(1696..1824)
                     /locus_tag="ACHBFW_001435"
     CDS             complement(1696..1824)
                     /locus_tag="ACHBFW_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317747.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(2169..2312)
                     /locus_tag="ACHBFW_001436"
     CDS             complement(2169..2312)
                     /locus_tag="ACHBFW_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFM9317748.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(2535..3014)
                     /locus_tag="ACHBFW_001437"
     CDS             complement(2535..3014)
                     /locus_tag="ACHBFW_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM9317749.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(3087..3371)
                     /locus_tag="ACHBFW_001438"
     CDS             complement(3087..3371)
                     /locus_tag="ACHBFW_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317750.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(3449..3643)
                     /gene="ssrS"
                     /locus_tag="ACHBFW_001439"
     ncRNA           complement(3449..3643)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHBFW_001439"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3715..5001)
                     /locus_tag="ACHBFW_001440"
     CDS             complement(3715..5001)
                     /locus_tag="ACHBFW_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFM9317751.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            5139..5810
                     /locus_tag="ACHBFW_001441"
     CDS             5139..5810
                     /locus_tag="ACHBFW_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFM9317752.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            5776..6264
                     /locus_tag="ACHBFW_001442"
     CDS             5776..6264
                     /locus_tag="ACHBFW_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFM9317753.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            6277..7224
                     /gene="prmA"
                     /locus_tag="ACHBFW_001443"
     CDS             6277..7224
                     /gene="prmA"
                     /locus_tag="ACHBFW_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFM9317754.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            7225..7989
                     /locus_tag="ACHBFW_001444"
     CDS             7225..7989
                     /locus_tag="ACHBFW_001444"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFM9317755.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            8222..10435
                     /locus_tag="ACHBFW_001445"
     CDS             8222..10435
                     /locus_tag="ACHBFW_001445"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFM9317756.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            10446..10892
                     /gene="dtd"
                     /locus_tag="ACHBFW_001446"
     CDS             10446..10892
                     /gene="dtd"
                     /locus_tag="ACHBFW_001446"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFM9317757.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            11063..12322
                     /locus_tag="ACHBFW_001447"
     CDS             11063..12322
                     /locus_tag="ACHBFW_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFM9317758.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(12411..13358)
                     /locus_tag="ACHBFW_001448"
     CDS             complement(12411..13358)
                     /locus_tag="ACHBFW_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM9317759.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            13752..15044
                     /gene="hisS"
                     /locus_tag="ACHBFW_001449"
     CDS             13752..15044
                     /gene="hisS"
                     /locus_tag="ACHBFW_001449"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFM9317760.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            15059..16828
                     /gene="aspS"
                     /locus_tag="ACHBFW_001450"
     CDS             15059..16828
                     /gene="aspS"
                     /locus_tag="ACHBFW_001450"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFM9317761.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            16937..17812
                     /locus_tag="ACHBFW_001451"
     CDS             16937..17812
                     /locus_tag="ACHBFW_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9317762.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
BASE COUNT         5643 a         3333 c         3827 g         5075 t
ORIGIN      
        1 aaacgtccta gtcaatcagt tggccgagcg gctggcggct gatctgctcc tgatcaataa
       61 cgagtgcttc ttggaagtgt tgctgcaaaa agtagcggtc atggctgact gctaggagcg
      121 ttccttgata gtcttgcagc atttcttcaa tttcttctcg agcatagctg tcgaggtgat
      181 tggttggttc atccaaaatc aggacgttga ttttttctaa aaagagtttc agcaacgcca
      241 aacggatttt ttcaccaccg gataaatctt tgatccgttt acccacatcg ctttgataaa
      301 agccactttt ggctaattgc cgtcgagcga cttcttcgtt tccagtcaat gtgatggcgt
      361 aagccagcaa acggctatcg ggatcggcaa actgcagctg ctgcggcaaa taaccgattt
      421 gcaagctact acctaatttg atggtgccac cgtaaggctt ttcctcaccg agtaataatt
      481 tgagtaaggt tgattttcct gcaccattgt cgccgatgat ggctaaatga tctccacgat
      541 agactgcaaa actgctgtct ttgaatagta cttgatcagc gtactgctgg tgcaagtttt
      601 gaatgatgaa aacttctttg ccagattttc caccattaga caagctttgc agccgatttt
      661 tcgggggagg tggcggtttg acgagtgtca gtttcgccag gcggcgttcc agttctttgg
      721 cttttttgaa gaaggactca ttgtctcctt catgtgccca ctgacgaaaa cgacgaatca
      781 tgactttcaa ccgctggact tctttttgct gcagttcgta attttttgtc agctcagcca
      841 agcgagcttc tttcaagact cgaaaacggc tgtagtttcc gggatactca atcaatgcac
      901 cttcttccag ttcccatgtt tttgtggtga cttgatcgag aaattgccga tcatgagaaa
      961 cgaccagaac cgattttttt gtattctgta aatagttttc caaccagcga atactgttaa
     1021 gatctaaatg attggtcggt tcatcgagta aaagtaaatc ggcatcggct gctaaaattt
     1081 tggctaaagc gactttaact cgttcgcctc cgctaagttc tgttaaccgt gcttcctgac
     1141 ggtgtggaat ccctaagcct tgcaagatcg ctgcaatccg gtcttctaat acgtaaccgc
     1201 ctagttcttc aaagcgatgc tgcagctcac cgtaacgggc cagacacttc tctaagtttg
     1261 tatctggttc tgtcatcaat gcttcgtagc gactcatttg ctgtttgagg tcctgtatct
     1321 cagcggcaga atggagcaag tagtggttga cggtcgcttc ggaaactgta agttcttgtt
     1381 cgaccgtccc gatctttaag ccttttttgc gactgataac accgctgtcg acaccttctc
     1441 tgccggtcaa gatttgtaaa aaggttgatt tgccagttcc gttgataccg actaagacaa
     1501 ttttttcacc agcttcaatt tgacaggata aatcctcaaa taatggattc ccgccgattg
     1561 tttttttaat agaaattgct tgtatgatca tgatctctct ccttgagacc actcaaaaag
     1621 gccatggtta tccatggcct gtgttattta aactcagatt atcatgagaa aaataccttg
     1681 agatggccat tgattttacg tgaattcaac tgttttggac aaacttgtcc cgtcaacagc
     1741 aactgtaaaa ccaattgcat ggctaaatgc gatatggtat cgttggtaat tttcattact
     1801 tgttatttta gtcatgcgca tcatagtatt ttccctcctt cgtgtagttg tcttttccag
     1861 tgtacctgaa agtgtcagaa agttcaacta gcgctgacat ttacttaata tttatcgatg
     1921 tgtaattctg tcagagagct ttttttatct gactgagggt aatgtctcgt ggaccacatc
     1981 attgtattct gagctaaacc agcgttcagg cgaaatcata tggtgtttga taaaaaactt
     2041 tgtctacatg acttcctttt gccatttgct taataccgtt gcattaaaca gctcttctct
     2101 ttggaaatct caccaacatc gcgttcagga gtatcgttgt ggttgcaatg gcaagcggta
     2161 gcctttcatc aattcttgaa tgatttaccg aatgtagcac ttgccttctc gcggtacccg
     2221 aacccgaaat aaaattttag tttggcgata aaatcggtat ccgccatcga tacttcatat
     2281 tttttcgtat cagattcatc ggaaacttcc acattgacga accaataagc tttggcatgc
     2341 ttaggtcgtt ttaaaccaca ccagggcgac taatcgccct ggtgttcact tttttttatt
     2401 ttacttgaac tcattgactg ttttcccttt attttaaaca ttataaagac cgatgagtgc
     2461 ttgccgcgtc ttttgttttt atcacggttt tttgtgcaaa aaaacctgcc gatagttggc
     2521 aggtcgtcta cgtgttaagc tttggtgtgg tctttagctg gcaattggtt ttctaaatca
     2581 gttcggacaa caagcacatc gcaagctgcg tttctaataa catattcaga aacagaacca
     2641 ataaataaac gttctacagc attcaatcct gtggctccta acataatcaa atctacttga
     2701 ttatcttctg ggatttctct ggcaataatt tgttttggtg agccatattc tacgacagag
     2761 gttacattat tcaagccagc tttcttcgca ttgctttcat aatctgccaa tgtttgcttt
     2821 gccatttcag tagcttgttc tgcaagcatt ccatcaaagg atgaaacggt ttggaaagaa
     2881 cgtgtgtcaa tgacatgtgc taatagaagt tctccgttat ttcggatagc tacattgacc
     2941 gcctttttaa aagctaattc agcttcatca gaaccatcta cggcaaccat gatttttttg
     3001 tattgttgta acataagcat tcactcctca agtcaatcgt attgtactct tttattgtag
     3061 aacactttag ggaaaaagag aattatttac tgctttggtt cagcaatttt ttttgcaaat
     3121 cggatactga aggtggcaga aacaataatt acgatcagta aaaagaccaa ggcaataaag
     3181 cgttgattga aaaaagctgt taaaataccc attacgccta aaacagcata tgcagctccg
     3241 taaaattgta ggaatccttg atttttatca tttttgttga tcaatacaaa aaagatatcc
     3301 tgtttattaa ataaataata gctaacgaat aataatacta aagtggcgat aatcattaaa
     3361 atcctaatca tgaacaaatc ctccaaaata agcaacaaat agaaaaagca atttgccaag
     3421 agcaaattgc tttattcttt aaaagatggt aatctccgtt aatgccgttg tttgtcctgt
     3481 agtattgatc ccgcgaattc aagcaggtgg gtcccatggt cgtagcttcg aactaccaaa
     3541 tgataaggca tgttaccagc agtgacacaa gacgggtccc aagatatcaa taaaaatgtt
     3601 cggtcaacac acaaagagga caattcgaac atcacagatt tcccacctct atagtagcat
     3661 atgaatttaa aaagagcaat gagagagctc gcagaaacag aaccgttttc ttgtctagtt
     3721 ttttggagga tgctttttcc actcttttat acggtaatac tgttgaccca gagcctgttc
     3781 atatttccct gtatcttttg gctgataata ctgagcattc ttcagtttat ctggtaaata
     3841 ttgctgattg acccaagcct gatcaaaatg atgaggatac tgataaccca cgccacggtt
     3901 gagtgatttc gcacctttgt aatggctatc tctcaaatga tcaggaacat ctccagcttt
     3961 gccctctcga atatcagcaa gtgctgcatc taatgccatg taggcagaat tggattttgg
     4021 cgacaagcaa agatcgacga cgacgtctgc tagtgggata cgtgcctcag gcaatcccaa
     4081 tttttcagca gctaatacag cgttcactgt acgagccgca gcagcaggat tacctaaacc
     4141 tatatcttca taaccgataa ccatcagacg ccggcaaata cttgccagat cacctgcctc
     4201 aactaaacgg gcgagataat gaagtgcagc atccacatca cttccacgaa tcgatttttg
     4261 aaaagccgaa ataacgtcat aatgagcatc tccatttttg tcatgggtaa gagcttttct
     4321 ttggacacat tcttcaatga tcgacagtgt aagatgaatc cttccttctt tatctgaagg
     4381 agtcgatagt gtagccagtt ccagcccatt caatgcactc cgcaaatcgc cgttcgttgc
     4441 acgcgaaagg tgaagaagag catcttcgtc taactggatt gcttgttgtc caaggccgcg
     4501 ttctttatcc cttaaagcat gttcgactgc caactggatg tcttgttctg ttaatggttt
     4561 gacttcaaag atctgtgtac ggctgcggat cgctggattg atggtaatat aaggattctc
     4621 agtagtcgct ccgatcaaga tgattcggcc actttctaaa tgaggaagca gaaaatcctg
     4681 ttttgtttta tctaatcggt gtacttcatc caataagagg atcactgtac cactcatttt
     4741 tgcttcttcc gctacaactt gtagatcctt ttttgaatct gtagcggcgt ttagcatgcg
     4801 aaatgcataa ttagtagagc cggcgatcgc acttgcgata ctcgtttttc cagtaccagg
     4861 aggtccatat agaatcatgg aagagagcat gcgagcatcg accatgcggc gtatgatttt
     4921 tccttccccg actagatgct gctgaccaac gacttcatcg atcgttcgag gtctcatgcg
     4981 ataagccaat ggctgttgca taggattttc ctccttcttg tttttacgtc ccttaattat
     5041 atcatattta gcaagatgcc tagaaagaac agccttctgt tgttttgttt tcatagctta
     5101 tccaactaat attgtgtatg ataaagtagg tgagaaaaat gacggataaa caaaagatag
     5161 atgaattttt ttggaataaa acgacgccag aagtggcacg ggatttattg gggatgtatt
     5221 tggagcatga cacaccagaa ggaagattag ctggatatat tgtagacgcg gaagcatacc
     5281 taggtccaga agacgaggcc gcccatagct atggtttaag aagaacaccg cgtgtgcggg
     5341 caatgtatga aaaaccaggt acgatctacc tttacacgat gcatactcat cggattttga
     5401 acatcattac tcagccagaa gggattcctc aaggagtaat gatacgtgca atcgaacccg
     5461 ccgcaatgat tgatcaaatg agtaaaaatc gtggcggaaa aactggtcca gatatcagca
     5521 acggtccggg aaaattagtg gaagcattag caattcctca agaactttat ggtcagtcta
     5581 tagcagatag ttcgttacgc ttagtttttg agaaaaaaaa gacaccaaaa aagatcaatg
     5641 ctttgcctag aatcggtatc ccaaacaaag gtgtatggac agaaaagccg ttgcgttttg
     5701 ttgtgtcggg aaatccttat atctctttac aaagaaaaaa ccaaatcgag aaagattggg
     5761 gttggaggaa agaaaatgaa aaagaaggat ctattaacat atttagatga aataattgaa
     5821 aaacaagtga cagactatga tgtagcttta gattgggata caaaaaatca tacgatcgag
     5881 atcgttgttc gcttgtttgc agaaaataaa gcacaatttg agattgatga tgcagaaggg
     5941 atcgtttcgc aagaaccaat tatcgaattt gaagacggcg tacttctttt taatcctcag
     6001 aaatctgttt ttgatgaacg caattatcta gcagtaatcc cttatgaagg aaaaaaaggg
     6061 ttggcaaaat cagtagctga ttctttagtg gaatatctaa atgaagtatt agctcaaggg
     6121 caaagcgatt tgcttgattt tttggatgaa gacgatgacg aagctgtatt cgagcttcac
     6181 tgggataatc agcaactagc agaattagta gaagtaaaac agcaaaatga agcagatact
     6241 tatttaccgt acccaagtta ttaaggagtg acagatatga aatggaatga agtaaaagta
     6301 gaaactgaaa gtgaagcagt cgaagcagtt gctaatattt tgatggaggc aggggcgagt
     6361 ggtgtcgcga ttgaagattc tttagatgtt gaacattttc agtcggatca atatggtgag
     6421 attttagata aaaatgattt tacatcgctg aaagaaggcg cactagtcat ggcttatttt
     6481 ccggaaacga ctttcttacc ggaaatcttg ccttttatca aagatcgtat cacacaatta
     6541 cctgagtttg gtctagctat tggaaaaaat aaagtaacag ttagtgaagt agaggaaagc
     6601 aactgggcaa cagcctggaa gaaatactat catccggtgc gtattacgcg aatgctgacg
     6661 gttgcgccaa gctgggaaaa ctaccagaca gaagatcctc tagaaaaaat tatcacacta
     6721 gatcctggta tggcatttgg cacaggcact catcccacga cttccttgac attgacagca
     6781 ctagaaacag tcttgcgtgg tggtgaaaca ttgctagatg tgggaactgg gtcgggcgtg
     6841 ctaagtattg cagcaaaata tcttggtgca aaagatgtct atgcctttga tttagacgaa
     6901 gtagccgttc gttcggcgaa agaaaatatg gatatgaatg aaatagccaa agatgtccat
     6961 gtatcagcta acgatctttt gacaggaatc gagattgaaa gtgatgtaat cgttgcgaac
     7021 attttagcag atatcatctt attgatgatc ccagatgctt ggcgtttact gaagcaaaca
     7081 ggcacgctga tcgtttcagg aatcattgaa gagaagaagc aaatggttct ggatgccatg
     7141 ttagaacaag ggtttatcgt ggatcaaatt ttccagcaaa aagactggta tgcaattatt
     7201 ttgaaaaaac cagaggtgga atagatgcag cggtattttt taaaagaatc atatgaatca
     7261 aaagactatt ttgttttaga aggggaaaac tatcaccaca ttgtacgggt tatgaggatg
     7321 gaacccgagg atgaagtttt tttggcattc aatgatcaaa tagccattgt tgcagaaatc
     7381 acggagatta ctgaagaggc tgtttatctg aaagaaatta aaaaagaaga aaatcaaaag
     7441 gaactgccaa gttcgattac catagcgagt ggctatccta aaggggaaaa gctggattgg
     7501 atcgttcaga aatccactga attaggagcg caagcttttc ttggtttccc ggcatcagcc
     7561 tctgtcgtta aatgggatgc aaaaaaacgt atgaaacgac aacaacggct tgaaaagatt
     7621 gctcaagagg cagcggaaca atcccatcgg cagatcgtgc catcagtaga gttattggaa
     7681 acgaaacaag atttatttga gcggctagat gattatgacg tggtcttggt tgcttatgag
     7741 gaatcagcaa agcaaggcga aaaatcaaat gcagccaaaa ttcttagtga aacaaaacca
     7801 gatagtcgaa tcttagctgt ctttggacca gaaggtggat tatcccgcga tgaagtcgat
     7861 tattttgtag aacacaaagg gatactttgt ggattaggac caagaatcct tcgtacagaa
     7921 acagcacctc tttatttatt aagtgctgct agctatcaat gggaattaat gaatccttct
     7981 ttcagctaac gttcttcatt aataaaagag tatcgacaga aagaattgaa aaaatagtca
     8041 acaatttgta taatagaaac aattatttat acaatggata cgaaaaatcg tttgaaaaag
     8101 cttttaaaaa gcgaacattt ccgtaaaaaa acgtataact gcacggattt tgggacaatt
     8161 atcaaacaga tatttgtccc gattcccgtt ttaccgaaaa aagaaagggg gatcgattcc
     8221 tgtgacaaaa gaagaaattt taacgggacc cggtgtcata aagctcgtta gtcaatacat
     8281 gggtcccgaa catgtagcat ttgttgagaa agcatgcgaa tacgcgactg ctgcacatga
     8341 cggacagttt agaaaatcag gcgaacccta cattattcat cctatccaag tcgcaggtat
     8401 attagcagat ttaaaaatgg atccccatac agtggctaca ggcttcttac atgatgttgt
     8461 tgaagataca gaaatcactt tagaagatct gagagaagaa tttggcgatg acgttgctat
     8521 gttagtggac ggcgtaacca aattagggaa aataaaatat aaatcccacg aagagcagct
     8581 agcagaaaac caccgaaaga tgctgctagc aatggctcaa gatttacgag ttatcatggt
     8641 caaattagct gatagactac ataacatgcg tacgttgaag cacctgcgag aagataagca
     8701 aagaaggatc gctcaggaaa ctttagaaat ctatgcgcct cttgcacatc gtctagggat
     8761 cagccggatc aaatgggaat tagaagatac agcacttcgt tatctaaatc caaaacagta
     8821 ttaccgtatc gtccacttga tgcagacgaa gagagaagaa cgtgaaaaat acgtaagtgg
     8881 tactgttgaa gatattcgaa tagcgacaga agagctgggg atttttgcag aaatctatgg
     8941 acggccaaaa cacatttatt caatctatcg taaaatgaaa gatcagaaaa agcagttcaa
     9001 cgaaatttat gacttgctag ctattcgagt gatcgtagat tcaatcaagg attgttatgc
     9061 tgtattagga gcaatccaca caaaatggaa accaatgccc ggcagattta aggattatat
     9121 tgccatgcca aaagctaata tgtaccaatc tttgcatact accgtgatcg gtccggcagg
     9181 aaatccggta gaaattcaaa tccgaacaca ggaaatgcat gaaatcgctg aattcggggt
     9241 tgctgcacac tgggcctata aagaaggaaa aaatgaaaaa gtagaaccag atggtatgac
     9301 gaaacaatta agctggttcc atgagatact cgaacttcaa gacgaaagct atgatgcttc
     9361 tgaatttatg gaaggcgtaa aaggagatat ctttagtgat aaagtctacg tcttcacacc
     9421 aaaaggagac gttactgagt taccaaaagg atccggacca ttggactttg catacagtat
     9481 ccatacagat atcggtaaca aaaccactgg tgcaaaagta aatggcaaaa tggtgcagct
     9541 tgattacaaa ttgaaaaacg gagatatcat tgagatcatg acttctccaa attcatttgg
     9601 cccaagtcgc gactggttga aattagttgc tactagcaaa gcaagaaata agatcaaacg
     9661 tttcttcaaa gcccaagatc gagaagaaaa tgtgatcaaa ggccacgaat ccgtggtcaa
     9721 atgtattaca gatctaggat ttacgcctaa agatattttg acgaagaaca aactgcaaga
     9781 agcactcgat cgttttaatt atcaaacaga agatgatctc tatgcagctg tagggtatgg
     9841 agaagttagc cccttgacga tggccaatcg tctgactgaa aaagaacgta aagaacaaaa
     9901 aatcgagcag caaaagcaag aagcagaaga aatcatgaat cagccgaaaa aagaacctga
     9961 caaaatgaaa gtacgtcatg aaggtggcgt tgtcattcaa ggggtagaaa acttactaat
    10021 tcgtatcagt cgctgctgta atccgattcc tggtgatgat atcgttggtt atatcactaa
    10081 aggcagaggg atatccattc atcgtcgaga ttgtccgaat gttcagcctg acaaaccaaa
    10141 tgtagcagaa cgtttgattg aagtcgaatg ggaagataca tcgaatacac gaaaagagta
    10201 tgatgcagat ttggaaattt acggctataa tcgttcaggc ttattgaatg atgtacttca
    10261 aacagtcaat gcgctaacga aaaatctcaa cagcgttgaa gcacggacga ataaagataa
    10321 aatggcgacg atccatttga cggttggtat ccagaattta tcccacctaa agagtatcgt
    10381 ggataaaatc aaagcagtac ctgatgtcta cagtgtacgc cggacgaatg gataggagga
    10441 cagatatgag agcagttgtt caacgggtaa gtaaagctgc tgtgtcagtc gatcagcaag
    10501 aaattggaaa aattggctac ggcctacttg ttttattagg cattcatgaa accgatacgc
    10561 aaaaagatgt ggactatctg gtaaaaaaaa ttgcacaaat gcgtattttt gaagatgaac
    10621 aaggaaaaat gaatattagt gtagaagatg tgggaggaca aattttaagt atctctcaat
    10681 tcacactgtt tgcggatacg aaaaaaggaa atcgaccaag ctttgtggaa gccgcacgac
    10741 cagaaacagc tattccttta tacgaatcat tcaatgaagg aatcagaagt cgcggcatca
    10801 cagtcgctac cggacaattc ggagcagata tgtcggtttc tctcgtcaat gatggacctg
    10861 taacgatcat tattgattct aaaaacaaat aaagtaatct ggttttcact agtaaaaact
    10921 atgaaacacg tcgcttaagt tagactgata aactctaact taagtggctt ttttgtctgc
    10981 aaaaagcgct tcttttgtca gcagtcggaa aaaaagttaa gcttaaatta tacaagttat
    11041 tttttatagg aggttgctga agatgaaaga agtaccagta gtagatatca aaatcacaga
    11101 tttaaaaaaa ttgtatcaaa aaggatacaa catgttggag gagttacgcc atgaagcgga
    11161 cgctcccgtc gtcaaagcaa agattttcaa taaagaagct atcacgatct acggatcatc
    11221 agcagcaaaa gtattttatg atccaaggaa tttcaaacga aaaggtgcta tgccaaaatt
    11281 agtattgaag acactttttg gtcaaggtgg agtacagact ttggatggag ctgctcatca
    11341 ccatcggaaa aatattttta tggatctaat gacaccggaa agaatggaag attatcatcg
    11401 tatcttagat aagaatctga cacaagcact tgaagcacag catggtcaat ttgaactatt
    11461 tgatttaagt aagatggttt tctttacatc tatttgcgag tgggcaggta tcaatttatc
    11521 agcgatttct aaagatgagg tcgaaaaatt agcagaatat caaatctcta tgatcagcgg
    11581 aacgtttact tctccgattg atcacataaa aggtgtcgaa aatcgcaaaa aatcagaaaa
    11641 atgggcgcaa ggcttgattg aagaagctcg acaaaatcct gtggctggaa aagaaaatgt
    11701 ggcattatat gcttttgcca atgccacaga tttagatggt cagcttttac ctctggaagt
    11761 ggcagctgtt gaattattga acatcatccg tccaacggtt gctctaactg tgtgggcggc
    11821 attgatggga catgcacttt tcagtagacc agatttgtat cagcaattaa aaaatgactt
    11881 ttcgacgtta caagatccgt ttattcaaga aatgagacgc tattatcctt tcttcccaat
    11941 gctacctgcg atttcgctga aagaagtaga agtagatggt tatcgtatac cagaaggcag
    12001 ttgggttatt ttagatttat atggaacaga ccatgatgaa cgaacagttg aagcgccaga
    12061 ttcctttatg atcaaacggt atgtagggaa agcaaaagat atttcctata aagaagaata
    12121 tgagatgatt gcacaaggcg ggggaaattt tcgacaaatg catcgctgtg caggagaatg
    12181 gatcactctt catagtctac gtgtgttttc cgatcagcta gtaaataagt ttgagttctc
    12241 tgtacctgaa caagattgga cgattccatt caatcagttt ccgacttatc cgaatagccg
    12301 agcgctactt tacaagaatt agattagcat aaacttatcg aaaaaaagga agatcaaaca
    12361 gtgctgatct tccttttttc atattactat aaattgataa ggtcaattga tcaaaatcca
    12421 cgaaatgctt catcaatggt ttgataatct tcattggata aatgcaaatc taaagcttgg
    12481 acattgtcag atacttgctc tggacgctta gctccaggta taactacaga cactcgtgga
    12541 ttcttcatgt accaagctaa aacaagttgc gcaggagtgg cttggtatct tttagcaatg
    12601 gggcgaagta catcgacagc ggttaaaatt tctctgaaac gttctccttg gaaatctgga
    12661 tttctttttc gaggatctcc ttcaccaaat tgtttttcct caccaagttc atattttcct
    12721 gtcagcaaac cggaagcaag aggaaaatag ggaacaaaac tgattttgtt tttttcaaga
    12781 taagggaata attctttttc agcctgccga tggatcaaac tgtatttatc ttctactaca
    12841 tcgacatatc catctgcatt cgcttctttc aattgttcta acgtgaagtt tgacacaccg
    12901 actgctcgga tttttcctgc ttcttttaat tcgtgcaatg tagcaactga ttcatttttt
    12961 ggtgtgcttt catccggaaa gtggatataa aaaatgtcga tgtaatcagt ttgcagacgt
    13021 tttagtgcat cttccaccga ttgtttgaga aactcaggag aattatcaaa tgttcgccct
    13081 ttatttggta catgagcagc ttttgtagcg atgacgacac gtgagcgatc atattctttt
    13141 tccttcagta cctcgccaat cagttcttcg gaacggccgt ttccgtaagc ataagcggta
    13201 tcgatcaaat tgatcccgct attcaaagct gtacgaacta cttgttttcc tgtctcatct
    13261 tccaaaccgt caaaaagatt atgtcctcca acggcatttg ctcctaatcc aagctgttct
    13321 gcgtagactt gtgaatgtcc gattcttatt tctgccatag ctgattcctc ctttttgtct
    13381 agtctaaaac aaaataccaa aaagctgaac taaaacgttt ttgtgagggg aaatagtcag
    13441 atatctcttg actttgagac tggtttttag taggttataa gtaatatcga atgtcaatga
    13501 aagagcgagt aagtttttga aactttacaa gagaggatca tcgaggctga aaatgatcta
    13561 aagtttcatt agcctaaaga cactctggag atggacgagt gaaaagccgt cccgcgtacg
    13621 agcgttattc gtaagaggaa agagaaatct ttcaaaatta ggtggtaccg tgtatgaatt
    13681 tcattcaccc ttgttcatga tgaacaaggg tgattttttt atttacatag ctaagaaagg
    13741 gagagaaaac tatgagtttc caaagaccaa aaggaacaaa tgatattttg ccaggcgaat
    13801 cagaaaaatg gcagtttgtc gaagaaacag cacgtttatt attcagagat tatcagtata
    13861 atgaaatgcg tacaccgatt tttgaacact atgaagtgat atcccgaagt gtaggggata
    13921 caacggatat cgtttcaaaa gagatgtatg atttttatga caaaggggaa cgccatgtga
    13981 cgcttcgacc agaaggtaca gcaccaatcg ttcgttcttt tgttgagcat aaattgttcg
    14041 gcccagagta tgcgaaaccg ttcaaaactt tttatatggg accaatgttt cgctacgaac
    14101 gccctcaaaa aggacggtta cgtcaattcc atcaaatcgg agtagaagca tttggatcag
    14161 aaaaccctgc aactgatgtg gagagcatgg tgatggcact tgatttcttc aaacagttgg
    14221 ggatcacgca aatccggcta gtcatcaatt cattaggtga taaagaaacg cgtcaagcct
    14281 atcgtcaagc attgatcgac tatcttttac cttttgaatc agagctgagt gaagactcta
    14341 aacgccgcct tcatgaaaat ccattacgcg tattggacag taaagacaaa cgagatcaaa
    14401 aatttgtggc agaagctcca tctatcttag attatctaag cgagcctgct caaaaacatt
    14461 ttgataccgt tgtttccatg ctggatgcat tggctgttcc ttatgaaatc gacagtaata
    14521 tggttcgcgg actagattat tacacacata cgatttttga aatcatgagt gacgcaccaa
    14581 aaatgggggc acaagcaacg atttgcgcag gcggacgtta tgacaatcta gtggaagaac
    14641 taggtggtcc ttcaacacct ggcttcggat ttgcaatggg tatcgagcga ttactcttga
    14701 ctatggaggc agaggaagta gttatccctg catttaatga gttggatgct tacgtggtcg
    14761 cactcggaga cgaaacaaat atcgaagcat tgaaagtggt ccaagcgatt cgaaactttg
    14821 gtttttcagc tgaccgcgac ttcatgaacc gtaaagcaaa agcgcaattt aagacagcag
    14881 ataaagcgaa tgccaaatta gtattggtga tcggtgaaga tgagcttgct agtcaaacga
    14941 ttaatgtcaa agcaatggca agccgaaaag aaaaaagttt tccattagaa gagatatatg
    15001 aacattttga tgaagtctat gatgaaatga ctttattcga gtaggaggaa aaaaataaat
    15061 ggcacaaaga acgatctatt gcggattagt ttccgaacaa caattagaac aaacagttac
    15121 attaaaagga tgggtacaaa aaagacgaga ccttggggga gtaatcttta tcgacctacg
    15181 tgatcgtgaa ggaatcgttc aaatcgtctt taatcctgaa atcaacaaag aagcatggga
    15241 aatcgcggat aaatgtcgca gtgaatacgt gatcgaagtg actggggaag ttagaagacg
    15301 cggagaaaat gcagtaaatc ctaaaatggc cactggtgaa ttcgaagtga ttgcaagtga
    15361 aatcgtgatt ttgaatcaag caaaaacccc gccatttcaa attgaagatg atcaagcaat
    15421 cagtgacgaa atacgtttga aatatcgtta tttggatctg cgtcgtccaa aaatgactaa
    15481 aaacatcaaa ttaagaaatg cgacaacaaa agcgattcgt cattatttag atgataatga
    15541 ctttctcgat atcgaaacac cttacctagg gaaatctaca ccagaaggcg caagagacta
    15601 cctagttcct tcacgtgtcc atgctggaca tttctatgcg ttgccgcaat ctcctcaatt
    15661 gtttaaacaa ttgctgatga atgctggatt cgaccgttat tatcaaatcg ttcgttgttt
    15721 ccgcgatgaa gatttacgtg gagaccgtca gcctgaattt acgcaagtag atattgaaac
    15781 aacgttctta acagcagaag aaatccaaac ttacacagag ggattgattg cgaaggtaat
    15841 gaaagaagtt cgcggtatcg aagtcacatt gccatttcct cgtatgacct atgatgaagc
    15901 aatggctcgc tacggcagcg acaaaccgga tacacgtttt gatatggaat tgatcgatct
    15961 aagcgataca gtcaaagaag tagaattcaa agtcttccaa atggctcttg aaaacggcgg
    16021 tgtcgtgaaa gcattgaatg ctaaaggcgc tgcagatcgc tactcacgaa aagacatgga
    16081 tcagttaggt caatatgtag gacaattcgg agctaagggg cttgcatggc tgaaagtaga
    16141 agaagacgga ttgaaaggtc caattgctaa attcatggga gaagcaacag aagcaatcat
    16201 taaagcaaca gatgcaaaac caggtgactt gctgatgttt ggcgcagata aaagtgaaat
    16261 cgtcgctgct gcattaggtg ctatccggac acgtttagga aaagaattag gactaattga
    16321 tgaatcgaaa ttcaatttct tatgggtaac tgattggcca caatttgaat tttcagaaga
    16381 agaaggtcga tatgtctctg ctcaccatcc attcacgatg ccaaaagaag aagatatccc
    16441 gttattggct gaagaccctg caaaagtcta tgcagaagct tatgatatcg tcttgaacgg
    16501 ttatgaacta ggcggaggtt ctatccgtat tcatactcgt gagcttcaag aaaaagtact
    16561 agaaactcta ggctttacaa aagaacaaat ggaagaacaa tttggtttct tgttaacagc
    16621 tttagattac ggattccctc cacatggcgg tattgcatta ggattggacc gttttgcaat
    16681 gttattagcc ggagaagata atattcgtga agtcattgcc tttcctaaaa acggaaaagc
    16741 agcagatgtg atgagtgatg caccaagtat ggtttcgcct ttacaattgt tcgaattgaa
    16801 tattgatgta acgaatatag aagaatagcc gataaaaagg taaaaaggat ttgtcgaata
    16861 cgcttctttt gacaaccttg atttaattga aaaacacttt ataatgaagc cataacatat
    16921 aaagcggggc ttttccatga aaaaaacatt cgaaaattgg gcttatcatg attacacgac
    16981 caagctttcg gtatctatcg tttatgcgtt gatcgcatca gtcgcattga atttctttta
    17041 tcagccagga catatttatt caagtggtat tactggtcta gctcagattt tgacgacttt
    17101 gtcaaaatca ctgataggat tcgaagtacc ggtatcaaca actttgtatt tactgaatat
    17161 acctttattt gtattggcgt ggttaaaaat cgggaagaag tttacaattt tcacttttat
    17221 cacagtcact ttgacctccc tttgtatcca tattttaccg gaaacagtcc tatctaccga
    17281 tccaatcatc tgtgcgttgt ttgggggggc tgtgatggga ctaggtattg ggctagcgtt
    17341 gagaaacggc ctatcatcgg gcggtctaga tatcgtgagt atcacgattc gtaaaaaaac
    17401 aggaaagtcg attggttcta tttcgatcta cttcaatgca ttgatcgttt ttgttgccgg
    17461 aattctattt ggctggcaat atatgtttta cagtacgttg tctattttcg tcagtggaaa
    17521 agtaactgat gtcgtttata ctaaacaaaa gaagatgcag gtaatgatcg taacgaaaca
    17581 tccagaaaaa gtagtaaatg aaatccaaaa ctgtatgcgt agagggatca cgatcatcaa
    17641 tgaagcagaa ggtgcttata agcatgataa gcagacagta ttgatgacga tcgttacacg
    17701 atacgaactg cctctactta gagaagcaat gaaagcttct gatccacaag cttttgtaag
    17761 tattgcagaa aacgtccaga tacttggtcg tttctatgag gatggcattt agatcgagtt
    17821 tgacaaaaat tcatgaaata ctgctgtaga cagacgttcg tttgtctgca gttttttt
//
LOCUS       DAYYME010000052        17733 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000052 DAYYME010000000
VERSION     DAYYME010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.052"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..834
                     /locus_tag="ACHBFW_001452"
     CDS             22..834
                     /locus_tag="ACHBFW_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFM9317763.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            831..1631
                     /locus_tag="ACHBFW_001453"
     CDS             831..1631
                     /locus_tag="ACHBFW_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFM9317764.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1792..3060
                     /gene="rseP"
                     /locus_tag="ACHBFW_001454"
     CDS             1792..3060
                     /gene="rseP"
                     /locus_tag="ACHBFW_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFM9317765.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3128..4837
                     /locus_tag="ACHBFW_001455"
     CDS             3128..4837
                     /locus_tag="ACHBFW_001455"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFM9317766.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5049..9401
                     /locus_tag="ACHBFW_001456"
     CDS             5049..9401
                     /locus_tag="ACHBFW_001456"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFM9317767.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9545..10018
                     /gene="rimP"
                     /locus_tag="ACHBFW_001457"
     CDS             9545..10018
                     /gene="rimP"
                     /locus_tag="ACHBFW_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFM9317768.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10042..11217
                     /gene="nusA"
                     /locus_tag="ACHBFW_001458"
     CDS             10042..11217
                     /gene="nusA"
                     /locus_tag="ACHBFW_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFM9317769.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11239..11532
                     /locus_tag="ACHBFW_001459"
     CDS             11239..11532
                     /locus_tag="ACHBFW_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFM9317770.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11529..11840
                     /locus_tag="ACHBFW_001460"
     CDS             11529..11840
                     /locus_tag="ACHBFW_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFM9317771.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11853..14159
                     /gene="infB"
                     /locus_tag="ACHBFW_001461"
     CDS             11853..14159
                     /gene="infB"
                     /locus_tag="ACHBFW_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFM9317772.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14186..14533
                     /gene="rbfA"
                     /locus_tag="ACHBFW_001462"
     CDS             14186..14533
                     /gene="rbfA"
                     /locus_tag="ACHBFW_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFM9317773.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14641..15145)
                     /locus_tag="ACHBFW_001463"
                     /pseudo
     CDS             complement(14641..15145)
                     /locus_tag="ACHBFW_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15310..17673
                     /locus_tag="ACHBFW_001464"
     CDS             15310..17673
                     /locus_tag="ACHBFW_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFM9317774.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6047 a         3147 c         3906 g         4633 t
ORIGIN      
        1 ttaagtatag gaggaaaaag aatgttacgt ttttttccgc aaaaaaacaa atatattcaa
       61 gaaaaaagcg aatttcaatt taataccgat ggaccaattc ctcagcacat tgccatcatc
      121 atggacggca atggtcgttg ggcgcaaaac cgtcgtttgc cacgagttgc tggccataaa
      181 gaaggcatgg aaacagtaaa gaaagtcaca aaaaaagcat caaggcttgg agtgaaagtg
      241 ttgacgcttt atgccttttc gactgaaaac tggaagcgac caaaagatga agtaagtttt
      301 ctcatgcaac tacctgtcga cttctttgat acgttcgtac cagaattgat taaagaaaat
      361 gtcaaagttc atgtcatggg ttatgaaaac gtattgccag aacatacgca agatgctgta
      421 agacgtgcaa ttgagcaaac aaaaaacaat actggaatgg tattgaattt tgctttgaat
      481 tatggaagta gagcagagat cgtgactgct gtccaagaaa ttgccgaaga agtagcaaaa
      541 ggagaaatac acgcagaaga aatcgatgat gaattgattg ctaagcattt aatgacaggt
      601 ttcttgccta aagagttaca agatcctgag ctgatgatcc gtacaagtgg agaagagcga
      661 attagtaatt ttctgttatg gcagatcgca tatagtgagc tgtattttac aaaagcgctt
      721 tggcctgatt ttgatggtgc gcatcttgaa gaagcaatcg cctcttatca aaatcgagac
      781 cgtcgattcg gcggtgtaaa aaaaacagaa gatgagaaag gggatcagtc atgaaacaac
      841 gtgtgatcac agcagctgtg gcgctagtct tgtttattcc gattatatgg atcggaggca
      901 tagcggttga gcttacagca gcaatcttag cggtagtagg agtttacgag ctgttccgga
      961 tgaaaggatt gacactctta agttttgaag gagttttatc cgccgttggt gcagtgttac
     1021 ttgttttgcc aaaagaaaga tggtttttct ttcttccaga gaatgtaagt acatttatgt
     1081 tattttatct tatagttatg attcttttag gaacatcagt tatttctaaa aatacgtata
     1141 cgattgatga agcaggtttt cctgtgcttg tcagcttgta tgtgggaata ggtttccaga
     1201 attttgtcaa tgcacgaaca gaaagcttgg ctgttttgat atttggtcta tttattgttt
     1261 gggcaactga tatcggagca tatatggttg gtcgacgtta tggtcagcat aagttaagtc
     1321 ctgatatttc gcccaacaaa acgattgaag gtgcgctcgg tggtatcggt agtgctgttg
     1381 tagtcgcatt tctttattta ttgctatacc cagcaaaaga actattcaat catggtatgt
     1441 ttgtcatgct tctgatgacg attatttttt cagcagtcgg acaatttggt gatttggtcg
     1501 aatctgcaat caaacgtcat tatgatgtga aagactcggg aaatatctta ccaggccatg
     1561 gtggaatctt agaccgtttc gacagtttgc tgtttgtatt tcctatcatg catttatttg
     1621 gcattattta gtaagtatac ctgcgtttct ttgtgaattt cataaaagac gcaggttttt
     1681 atttttcatt atattgatac tattggtgtc tttggtatac tatggtaaaa ttatagtgtt
     1741 ttatcgttgt tttataaaaa acacaaaaaa caagtaaagg agaaaaaagc tatgaaaacg
     1801 attctgacat ttatcatcgt ttttggtata ttagtgattg ttcatgagtt tggtcatttc
     1861 ttctttgcga aacgatcagg aatcctcgtc cgcgaatttg ctatcggtat gggtccgaaa
     1921 atttatgggc atcaagcaaa agacgggaca acatatacgc ttcgcctatt acctattggc
     1981 ggttatgttc gaatggcagg caatggggat gatgaaacag aaatggcacc aggaatgcct
     2041 ctttctcttt tactaaattc tgacggcatc gtagagaaga taaatttaag taagaaaatc
     2101 caattgacaa atgctatccc gatggaattg agccgttatg atttagaaga tgagctgact
     2161 atcactggtt atgtgaatgg agacgaaaca gaagtagtaa cctaccctgt tgatcacgat
     2221 gcgacgatta ttgaaaacga cggaacagaa atcagaattg cgccaaaaga cgtacagttc
     2281 caatcggcta aattgtggca gcgtatgctg accaactttg ctggacccat gaataacttt
     2341 attttggcaa tcgtgttatt catcattctg gcatttatgc aaggtggtgt tcaagtgacg
     2401 aacactaatc gcgtaggaga gatcatgcct aatggagctg cggcagaagc aggcttaaaa
     2461 gaaaatgatg aggtagtcag tgtggacgga aaagaaatcc attcatggaa tgatctgaca
     2521 acagtcatta cgaaaaatcc aggtaaaaca ttagacttta agatcgaacg agaaggtcag
     2581 gtgcaatctg tcgacgtgac accgaaatct gtagagtcaa atggcgaaaa agttggccaa
     2641 cttggtataa aagccccaat gaatacaggt tttatggata agatcattgg agggacgcgt
     2701 caagctttca gcggttcatt ggaaattttc aaagccttag gatcactatt tacaggtttc
     2761 agtttagata aattaggcgg acctgtcatg atgtaccaat tgtcttcaga agcagcaaat
     2821 caaggtgtaa caactgttat tagtttgatg gcgcttcttt caatgaatct cggaatcgtc
     2881 aatctgcttc cgattcctgc cttagatggc gggaaattag tcttgaatat ttttgaaggg
     2941 attcgcggga aaccattaag tcaagaaaaa gaaggaatat tgacattagc tggctttgga
     3001 tttttaatgt tgttgatggt gctagttaca tggaacgata ttcaacgatt ctttttctag
     3061 aaaaagttga caaatagggt ataataacaa acgataacta ttttgaggaa ataaaggagt
     3121 atatagaatg agacaatcaa aaatgttgat tccaacatta cgcgaagttc caaatgatgc
     3181 agaagtatta agccatcaaa tcttgctaag agctggatat atccgccaag tatcagcggg
     3241 tatttattca tatctgccat tagcaaatcg tgtattggaa aaactgaaac gcattatgcg
     3301 ggaagaattt gaaaaaatcg gagcggtaga aatgctgatg cctgccatct tgccagcaga
     3361 actttgggaa gaatccgggc gttacgaaac atatggacca aacttgtatc gtttcaaaga
     3421 ccgcaatgat cgaaaaatga tccttggacc aactcacgaa gaaactttta ctgatttgat
     3481 gcgtaatgaa atcaattcat ataagaaatt acctttaaat ctttatcaaa tccaacctaa
     3541 atatcgtgac gaaaaacgac ctcgcttcgg tttgcttcgc ggaagagagt ttatcatgaa
     3601 agatgcatat tctttccatg caacggaaga aagtttggac gagacatata aagattacga
     3661 aaaagcctat accgaagtat tcaagcgttg tggcttagaa tttcgctcga tcatcggaga
     3721 cggcggcgca atgggcggga aagactcaaa ggaattcatg gcgatctcag aaatcgggga
     3781 ggatacgatc tgttattcga ctgaaagtga ttatgctgca aacttggaga tggctacaag
     3841 ctactatgta tcaaaaaaat cgcatgaaac acagcttgaa ttggagaaag tagctacacc
     3901 taacgtaaaa tcaattgacg aagtagcaga tttcttttct gttgaacctc aaaaaatcat
     3961 caagtctgta ttgttcattg cggatgaaca acctgtcctt gttttggtac gtggagacca
     4021 tgaagtcaat gacgtaaaac tgaaaaactt tttatctgct gactttttag aagaagcaac
     4081 agaagaagaa gcacaaaaat atctaggtgc tgagtttggt tctgtcggac cagtcggtgt
     4141 ttcagatgac gtaaaagtat atgcagaccg ttatgtccaa gatttagcga atgccattac
     4201 tggagcgaac gaaacaggat tccactataa aaatgtgaat ccaaaacgcg atttcactgt
     4261 gctttcttat gaagatcttc gttttgtaca agaaggagat ccttcaccag acaacaacgg
     4321 tgttcttgcc tttactcgag gaatcgaaat cggacatata ttcaaaatcg gcactcgtta
     4381 cagtgaatct atgggagcaa ctgtactaga cgaaaatgga cgagaaaaat ttgtgattat
     4441 gggctgttat ggtatcggtg taagtcgttt attatcagca atcgtagaac aacattcaga
     4501 tgaacaagga atcaattggc caaaaggaat tgcgccattt gatctgcata tcgtccaaat
     4561 gaatctaaaa gacgaatacc aaacaagcct gacgaatgaa ctagaagaag cgatgacaaa
     4621 agctggctat gaagtactgg ttgatgacag aaatgaacgc gcaggagtga aattcgcgga
     4681 ttctgatttg attggctgtc caatcagaat cactgtcggt aaaaaagcag tggatggaat
     4741 cgtagaagtg aaaatcaaga aaactggcga aatggttgaa gtacgtaaag aagaattggc
     4801 gaatacttta ccaatcctct tgaaccaagg cgaataataa gcgttaaaac acagaaaata
     4861 aaaatagctg aatcagctgt ttttgacaat ctgatgattc gcttttaaaa ttggaactgc
     4921 gatttgaatc gcagttccaa ttttttaatg taaaactcag aaaaaacgag tatactaaaa
     4981 ggtactgaaa aagaagagga taagatgcga tcaattgcat tgtttctcat cagaaagggc
     5041 gtatatcttt gtcagaaaaa cgagatttat ttgaaaaact gttggaacaa atccaactag
     5101 aggaagcaga aaaaaatcat ccactgctta ctgctggtga aatggatagt gtagtggtcc
     5161 atcgtaaatc gcgtttatgg gaatttacgc tacatttttc caaaattttg cctattatgc
     5221 tatatcggtc attgactcag catctgacta tcgcttttaa agatattgca caagtcaaac
     5281 tgaatatctt agcagatgac cagacattcg atgaacagct cttgcaagat tactgggccc
     5341 aagctttgga aaatcagcaa tgcgatacac cactagcaca gcaagtattg aaaacgcagg
     5401 tacctgtgat aaaagatcgt aaagtcatct tgccaatcga cagtacagga gctatttctt
     5461 atttgaagca acaatactta ccattagtag aagagttgtt cgtcagttac ggattcccaa
     5521 aatttcgtat cgaaccagaa gtggatgagc agcaagcgga acgtgtattg aaactttttg
     5581 aagaacgcaa acaagaacag gcagaagcct ttatgaagca agcagctgaa agtctagttg
     5641 tccacgaaca aaagaaaaag gaaagaaaag agcaatcgcc agctcttgaa ggacccatcc
     5701 agctaggacg aaatattcca gccgatgaac caacgacacc aatgatcagt atcgttgaag
     5761 aagaacgaag agtcacactt gaggggtacg tattcgacaa agaagtcaga gaactacgtt
     5821 caaaacgcaa gatcttgact ttgaaaatca cagattatac ttcatcattt atcgtcaaaa
     5881 aattctcgaa cggcgaaaaa gatgaacaag tatttgatgc gatcagtgtc ggtagctggt
     5941 taaaggtacg aggaagtatt caagaagata cgtttgtccg tgatttggtc atgaatgcgc
     6001 aagatatcat agaagtcaaa catacacctc gcaaagatta tgctcctgaa ggggaaaaac
     6061 gagtagagct tcatgttcat agcaatatga gtacgatgga tgcaacaaac agcatctctg
     6121 atcttgtggc acaagcagga aaatggggac accgagctat cgccattaca gaccatggtg
     6181 gtgcgcaagc ctttccggaa gcacatagtg ctggaaaaaa agcaggtgtg aaaatcttat
     6241 acggcgtgga agcgaatgtc gtagatgatg gtgtcccaat tgcttataat gatgcacatg
     6301 aagcattgag tgaagccaca tacgtcgtat ttgacgtgga aacgacagga ttgtctgcag
     6361 tatatgacac gatcatcgag ctagccgcag tcaaaatgta taaaggaaat gtgatcgaaa
     6421 gctttgatga atttatcgat ccaggacatc cattgtcacg aacaacggta gatctgactg
     6481 gtatcacgga tgaaatggtt cgagggtcaa agtcggaaga agaagtattg cggatgttcc
     6541 ttgaattttc aaaagataca attttggtag cccacaacgc ggcttttgat atggggttct
     6601 tgaatactag ctacgcaagg tatggtattc ctgaagcggc caatccagtc attgatacgt
     6661 tggaattagc tagatatctg tatcctcaat tcaaacgttt cggattaggt gtgttatcta
     6721 aaaagtttgg tgttagcttg gaacagcatc accgagcaat ctatgatgct gaagcaactg
     6781 gtcacttagc atggatcttt gtgaaagagg caatggacaa tcataacatg ctttatcatg
     6841 atcagctaaa tgagcatatc ggagaaggag attcttacaa aagggctcga cctttccatg
     6901 taacgatttt agcaaaaaat caggcaggac tgaaagattt gttcaaactg atttcaatgt
     6961 cgaatgtcga atacttcgaa cgcgtcccac gaataccacg ctcccaattg aaaaagatgc
     7021 gtgaaaatct attgatcggc tcagcttgtg ataagggaga aatattcgaa gccatgatgc
     7081 aaaaaggggt agaagaggca agaaaccgag caaaattcta tgattatatt gaagttatgc
     7141 caaaagcggt ctatgcgcct ttgatcgagc aagaattggt aaaaaatgaa catgatttgg
     7201 aagaaattat ccaaaatttg gtagaaatcg gaaaaagttt ggataaaatc gttgttgcaa
     7261 caggtaatgt acattatctg aacgaagaag atgccattta ccgaaaaatt ttgatcaatt
     7321 ctatgggcgg agcgaatccg ttgaatcgtc atagtttgcc ggatgtccat ttccgaacaa
     7381 ccgatgagat gctaactgca tttcactttt taggggaaga aacagcgaaa gagatcgtag
     7441 tagaaaatac gaataaaatc gcagatatct gcgaagaagt gattccagtc aaagatgaac
     7501 tttatacacc gaagattcca ggttcagaag atgaaatatc agaattgagt tacacaaaag
     7561 caaaacaaat gtacggagat ccgttacctg agatcattca aaaacgatta gaaaaagagt
     7621 tgaattctat taatggtaat ggcttctcgg ttatttatct gatcgcccaa aaactagtgc
     7681 ataaaagtaa tgaagatggc tatctagtag gttcacgtgg ctctgttggc tctagctttg
     7741 ttgcgacgat gacaggaatc actgaagtta atccattagc ccctcattat tattgtccag
     7801 agtgccaata ttccgagttt tttgaagatg gtacgtatgg ttctggattt gacatgccag
     7861 aaaaacaatg tccaaaatgt ggtgcacgct taaacaaaga cggtcatgat attccatttg
     7921 agacattttt aggattccat ggagacaaag tgcccgatat tgatttgaac ttctcaggtg
     7981 attatcaagc agaagcccat aactatacga aagtattgtt tggcgaagac tatgtttacc
     8041 gtgccggaac gattggtaca gtcgccgaca aaacagcata tggctatgta aaaggatacg
     8101 aaagggacaa taatctgcag tttcgaagtg cagaagtcga cagattagca aaaggcgcta
     8161 ctggagtcaa acggacaacc ggccagcatc caggggggat catcgttatt cccgattata
     8221 tggatgtcta tgattttacg ccgatccaat atccagcaga cgatcaaaat tcagaatgga
     8281 aaacgactca ttttgatttc cactcgattc acgataatgt attgaaatta gatattctag
     8341 ggcatgatga cccaactgtt attcggatgc ttcaagattt atcagggatc gacccacaaa
     8401 cgatccctac ggatgacccg gaagtgatgc ggatatttgc cggtcctgaa gtacttggag
     8461 taagtcagga acaaatttat tccaaaacag ggacattagg tattccagaa ttcggcactc
     8521 gttttgttcg agggatgtta gaagaaacgc atcctacaac ttttgcagaa ctactgcaaa
     8581 tttccggact atctcatggt acagacgtat ggttaggaaa tgcggaagaa ttgattcgac
     8641 gaggagatgc gacactggcg gaagtaatcg gctgtcgtga tgatattatg gtttatttga
     8701 tccatgctgg attagatagc gggatggcat ttaaaatcat ggaaactgtt cgtaaaggac
     8761 aatggaacaa gatccctgat gaattgcgag aaacctatct atcggcgatg aaagaaaata
     8821 atgtgcccga ctggtatatc gattcttgtt caaaaatcaa atatatgttt ccgaaagccc
     8881 atgctgcagc ttacgtattg atggctcttc gtgtcgctta tttcaaggtt tatttcccta
     8941 ttttgtatta ctgtgcatac ttttctgtac gtgcagacga ctttgatcta gtctccatgt
     9001 gcaaaggaaa agatgctgtg aaacaagcaa tgaaagaaat cacggacaag ggattagatg
     9061 cttccgtaaa agaaaagaat caactgacag tgctggagtt agccaatgaa atgctggaac
     9121 gaggattcaa atttggaatg attgatttgt ataaatcgga tgccgttaac tttgttattg
     9181 aaggtgatac gttgatcgca ccatttagag ctgtcccaag tttgggaacc aacgtggcga
     9241 aacaaatcgt tgaagcaaga aaagacggtc catttttatc caaagaagat cttgccaccc
     9301 gcggtaaagt ttcaaaaaca ttgatcgaat atatgaacga caatggcgta ttgaaagatc
     9361 taccagatga aaatcaatta tcgttattcg atatgctata agcatctgtt ttcctgtctg
     9421 cttgtaattt taagcagagt atggtatagt tcttataggt aatgatacta actgtgagtg
     9481 agcggatttt tcgctcactc tttttaatgg aattacgcac ttaattttta aggaggcgaa
     9541 acttttgagt agcgtcgttg aaacagtcac agaaatggtg acaccgattt tagatgaaca
     9601 aaattttgaa cttgtcgaag tcgaatttgt aaaagaagga aaaagctggt tcttacgagt
     9661 atttattgac aaagaaggcg gtatcgatat cgaagaatgc gcctttgtca gtgagaaact
     9721 gagtgaaaaa cttgatgcag cagatccaga tccgattcct caagcttatt tcctggaggt
     9781 atcctccccg ggagcagagc ggccattgaa aaaagaaagc gactatgaaa aggcacttgg
     9841 tgattatgtc catgtctcct tataccaacc agtagatggc gaaaaacaat atgaaggatt
     9901 tcttcaatca tttgatgctg aacagcttac attgaaaata cgcataaaaa cacgagaaaa
     9961 agaattgact tttgaccgaa aaaatattgc aaaagctcgc ttagctatcc aattttaatc
    10021 acggagagga atcagaaaaa aatgagtaaa gaaatgttaa atgcgttaga cgctttagaa
    10081 gctgaaaaag ggatctcaaa agaaatcgtg atcgatgctt tagaagcagc attggtttct
    10141 gcatacaaac gtcactatgg ccaagcacaa aacgtcgaag tggaatttga tcagaaaaaa
    10201 ggaaagatcc atgtctatgc ggtaaaagaa gtaacggaag aagtgatgga ttcacagttg
    10261 gaagtctcat tgaaagatgc acttttaatc aatccagcat atgagatcgg tgatacgatc
    10321 cgttttgaag taacaccaaa agattttgga cggatcgctg cgcaaacggc taaacaagtt
    10381 attttacagc gtgtacgtga agcagaaaga acgattattt ataacgaatt cagtgcttat
    10441 gaaaaagaca tcatgcaagg aatcgtcgaa cgccaggata aacgttatat ttatgtcaat
    10501 cttggtaaaa ttgaggcagt tctttctaaa caagatcaaa tgccaaatga attttatcaa
    10561 ccacatgatc gaatcaaagt ttatgtttcc cgagtagaaa atacgtctaa aggtccgcaa
    10621 gtatttgtta gccgcagcca tccagaccta ttgcgtcgct tatttgaaca agaagtacca
    10681 gaagtctatg atggaatcgt tgaaattgtc agcatcgccc gtgaagctgg cgatcgttca
    10741 aaagtggcag ttcgctcaaa tgatccaaat atcgatgcag tcggtacatg tgtcgggcca
    10801 aagggacaac gagttcaagc aatcgtcaat gaattaaaag gcgaaaacat ggatatcgtc
    10861 gaatggaatg aagatccagc tgtattcatc gcaaatgcat tgaatcctgc acaagtcatg
    10921 gatgtgatct tcgatgagac aaatccaaag gcctgcacag ttgtcgttcc agactaccaa
    10981 ttatcgctag caatcggtaa acgcggacaa aatgcacgtt tagctgctaa attgactaac
    11041 cataaaatcg atatcaaatc agaatctgac atgacggaat tttatgaaaa tcaagaacaa
    11101 caaaaagaaa cagaagaact gcatgatgaa gcaatcgttc agtcagattt aacagacgat
    11161 gaatatgaaa caattgcatt caacaatgaa acggtcgaag aaaaaccaga agcttaactt
    11221 tagtaaggag gcaaagagat gaaacaaagg aaaattcctt tacgcaaatc cgtggtttca
    11281 ggtgaaatga aacctaaaaa agagatggtc cgaatcaccc gttcaaaaga aggggaagta
    11341 tcgatcgatc caactggaaa aatgcctgga cgaggagcgt atgtctcact tgagccggaa
    11401 gaagtacaac aagcttggga taaaaaaatt ctcgatcgag tactcgaaac aacattaaca
    11461 gatgagtttt atcaagaact tcttgactat gtcactcacc aaaaagcacg taaagagtta
    11521 tttggaaaat gaatacagtt aatcggcaaa aagccatgaa tctcatcggt ctagcgatgc
    11581 gcgcgggaaa actaatcacg ggtgaagaac tgaccattgc tgaaatccgc aaacaaaaag
    11641 caaaattggt gtttgtggca accgatgcaa gtgagaatac aagaaaaaag ataaaagata
    11701 agagttcata ttatgaagtt ccttgctttg agctattttc tgaagcagaa atcacgcaga
    11761 tgatcggaaa acctcgcaag gtattcggaa taatggatgc cggatttgcg aagaagacca
    11821 aggagctaat tgaaggttag gaaggtgatt gcatgggcaa caaaagaatc tatgaattag
    11881 caaaagaact caaaaaatct agtaaagacg tcgtggaaaa agcacaacaa cttggcatcg
    11941 atgtgaaaaa ccatatgggt gcgattacta gcagtgatga gaagaaattg caacaagcgt
    12001 tcaaaaaccc aagcacgaaa caacaagctg cacaacaagc aagcagaaaa ccagccaatc
    12061 aacagccgaa cgatcaacgt aaaaaagaaa caacgaacaa tcaacaaaaa aataaaaata
    12121 accgcagtta tcaagatcgc ggtcaaggga gcggtcaagt gaatcaagga aaaaaaccaa
    12181 caacaaacca aaataatacg ggaaatacca gcagccaaaa tcgtcaagga acgagccaag
    12241 gcggcaatca aaaccgtact aacaataaca acaaaccaaa caataacaat cgcggcaaat
    12301 ttaataataa taaccgtaat cgttataata aaaaaggtaa aaaaggcaaa cagcaacaat
    12361 caactaaacc agctgtacca ccacgcaaat tccgtgaatt gccagatgta ttagagtata
    12421 ctgaaggtat gaacgtagca gatattgcga aaaaaattca tcgtgagcca gcagaaatca
    12481 ttaaaaaatt atttatgctt ggtgtcatgg tcaatcaaaa ccaagcactg gataaagata
    12541 cgattgaact gttagcgacg gactatggca tggaaccaca agaaaaagtc caagttgaca
    12601 ttgctgatat cgataaattc ttcgaggcag aagaagtcga tccagataaa ttagtctcac
    12661 gtccgccagt agtaacgatt atgggacacg tagaccacgg gaaaacaacc ttattggata
    12721 cattgcgcca ttcacgtgta acaagcggcg aagccggcgg aatcacacag catatcggtg
    12781 cctaccagat cgatattgat ggtaaaccaa tcactttctt ggatacacca ggacatgcgg
    12841 cctttacaag tatgcgtgca cgtggagcaa gtatcacaga tatcacgatc ttagttgttg
    12901 ctgcagatga tggcgtcatg ccacaaacag ttgaagcaat caaccatgcc aaagctgcag
    12961 gcgttccaat catcgtcgct gtaaataaaa ttgataaacc aggtgcaaat cctcagcatg
    13021 ttatgcaaga attaagtgag tatgaattaa ttcctgaagc atggggtgga gaaacgattt
    13081 ttgttgagat ctcagcgaaa ttcggccaaa acatcgaaga attgctggaa atgatcctgt
    13141 tagtcgcaga agtagaagat ttaaaagcag atccaacaca acgcgcaatc ggaacagtga
    13201 tcgaagcacg tcttgataaa ggtaaaggtc ctgtgtcaac cttacttgtc caagaaggaa
    13261 cacttcacgt tggtgaccca atcgttgtcg gtaatactta cggacgtgtc cgtgtaatga
    13321 caaacgatct tggccgtcgc gataaagaag caggacctgc aacgccagta gaaatcactg
    13381 gcttgaatga tgtacctcaa gcaggtgacc gtttcgtagt cttcgaagac gaaaagacag
    13441 cacgtgcagc gggtgaagaa cgcgcaaaac gtgcattatt ggaaaaccgt gctgccacaa
    13501 gccgtgtgac tttggacaac ttatttgaaa gcctgaaaga aggcgaattg aaagaagtta
    13561 acgtaatcat caaagcagat gtacaaggtt cagcagaagc actggctgca tcattgaaga
    13621 agattgatgt ggaaggtgtc cgtgtaaaaa tcgtccattc agctgtcgga gcaatcaatg
    13681 aaagtgatgt aaccttagca gcagccagca acgctattat catcggtttc aacgttcgtc
    13741 ctacaccgca ggcaaaagtg caagctgata ctgaagaagt cgatattcgt cttcaccgta
    13801 tcatctataa agccatcgaa gaaatcgaaa ctgcgatgaa agggatgcta gaccctgaat
    13861 ttgaagaaaa aatcaccggt caaatgatcg ttcgtgaaac cttcaaagtt tctaaagttg
    13921 gtacgattgc aggtgcctat gtaacagaag gatatattcg tagagatagc ggtgtccgtg
    13981 taatccgtga cggaatcgta atctatgaag gccaacttgc aagtctgaaa cgattcaaag
    14041 atgacgtgaa agaagtcaaa atgggttatg aatgtggtgc gatgattgaa aaattcaatg
    14101 acatcaaagt agatgacgta attgaaggat ttatcatgga agaagtcaaa aatgactaaa
    14161 aactgataaa aggaggccaa tcattatggc taactaccgt gatcgccgag tcggtcaaga
    14221 aatcttaaaa gaagtcaatg atattttgcg caaaaaggtc cgtgaccctc gtgtggagaa
    14281 cgtaacgatc acagatgtga aagttacggg ggacttacag caagcaacga tctattacag
    14341 cattttatct gacttagcat cagataagaa aaaagcacaa gatggattga ataaagcatc
    14401 aggattgatt cgtcgagagt tagggcacaa tctaagtatt tataagacac ctgaattgtt
    14461 ctttgaactt gatgaatcag ttgtctatgg gaaccatatc gatgaattgc ttcgcgactt
    14521 gaacaaagac taatcaaaaa atcccgcaaa tcctgtgaaa tcaggggttg cgggattttt
    14581 ttgttttcat taagacgtat tacctaagag ttccatgact ttagtctggt agcgtgtcag
    14641 ctaaccaaaa aagagaaaaa gaaaagtaaa aaattcgact ttcttttttc tatagtatat
    14701 aaatttttgc atatagataa agatcaagat accgataacg gaagctagat tgtaatctat
    14761 tttagtgaca gtcaggtata gccaagtaac tagaagatca gtggaacctg caccataaaa
    14821 gtttgagttt gtcggtccgt tgacagttaa aagaaaaatc acgttgaagt tattgatatt
    14881 tccgattacg ccgcttcgat ttggtcacga tccaaatttt ggataatccc tgtagaaatt
    14941 agcatagtag ctgggatacc aattcacata ttgacaaaga taacagagac ttttacataa
    15001 aacggatcaa tcaggaaagg aattgctcga tccagcccca gttttccaac aatgtgttaa
    15061 ctggcctagc gtcattcagc aaattatgca tgatgaggag cgaaatgaac tgcggaacag
    15121 ccattgtgat aacgacaagt tttttggatc aatgtaaaaa acgtcagcga aaggaaaaat
    15181 ttcgtggttt tttatacctg ttcatttggc tagatataaa atccaaacaa atgaacagaa
    15241 gtgcttatta ggtttcaatt gggagtatct gcgctagaat aagagtaata atgaaaagga
    15301 gtgttatgta tggattattc tagtaaagaa tactttgata aaatgacggc ttggtggcgt
    15361 gctgctaatt atctttctgt tgggcaactt tatcttaaag ataacccctt actgagacgt
    15421 acactgaaac cagaagatgt caaaaaacat ccaatcggtc attggggaac gattcctggc
    15481 caaaatttta tttatgtcca tttgaaccga gtcatcaata aatatgattt gaacatgttt
    15541 tatatcgaag ggccaggaca cggcggacaa gtcatggtat cgaatgctta tttagatgga
    15601 agttacacag agatctatcc agaagtaacg gaagatgaga caggaatgca aaaattattc
    15661 aagcgatttt catttcctgg aggcattgct tctcatgcag caccagaaac accaggttct
    15721 atccatgaag gcggagagct tggatactct ctttcacatg cagtcggagc tgtattagac
    15781 aatcctgaag taatctctgc tgtcgtgatt ggtgatggtg aagcagaaac cggtccttta
    15841 gctggaagct ggttctcaaa tgtctttatc aatccagtga tagatggagc tgttttaccg
    15901 atccttcatt taaatggtgc aaaaatagcc aacccaacga tcttggcacg taaatcagat
    15961 ggagaattag caaattactt taacggactt ggctgggagc ctttctttat tgaaggaaat
    16021 gatccagaaa aattaaatcc tgttatggct gaaaaaatgg atcaagcaat cgagaaaatc
    16081 aaatccatcc aaaaagaagc tcgcctgaag acagcaacag atgtcgtgat gccaaaatgg
    16141 ccagtattga tcgtacgcac accaaaagga tggaccggtc caaaagaatg ggacggggaa
    16201 ccaattgaag gaaccttccg agcacaccaa gtaccgattc ctgtagatca agaacatatg
    16261 gatcatgcag atgctttact ccgttggctg aaatcctatg aaccagaaaa attatttgat
    16321 gcacaaggac gtattttaga agaaatcaga gaaattgcgc ctactggtga ccaaagaatg
    16381 gcaaaaaatc ctatcaccaa tggtggaatc gatccaagac cgcttattat gccagattgg
    16441 aaaaaatata ctttgcaatt tgaaaaacca ggctccgtta aagcagaaga tatgaccgaa
    16501 cttggtaaat tcgttcgcga gatcattgag aaaaatccag aaaatttccg tatatttgga
    16561 ccagatgaaa caaaatcaaa ccgattgaat caagttttca aaacgacaaa tcgccaatgg
    16621 atggaaaaaa tcgaaccaga aaatgatgaa tggctttcgc catctggtcg cgtcatcgac
    16681 agtcaattat cggaacacca agatgaaggg ttcttagaag ggtacgttct gactggacgg
    16741 catggttttt ttgcaagtta cgaatcattc ttgcgagtgg ttgactctat gttgactcag
    16801 cacttcaaat ggatacggaa atcacatgac ctttcttggc gaaaagatta tccttcgctg
    16861 aatctcattg caagctcaac agtcttccag caagatcaca atggatatag ccaccaagat
    16921 ccaggaatat tgacacattt ggcagaaaag aaggcggagt tcatccgtga atatctgcct
    16981 gcggatgcaa atacattgct cgcagtcatg gataaagcat tccgctcgtc agaaaaaatc
    17041 aatttgatca tctcttccaa acatccgcgt gctcaatttt actcagcaga agaagcagcc
    17101 gtcttagtga atgaaggctt gaaaatcatt gattgggctt cgacagcaaa agaggaggaa
    17161 cctgaattgg tcattgctgc tgcaggaacg gaatctaact tagaagcatt agcagctgta
    17221 actttgctgc tggaagaatt cccaaaacta aaaattcggt tcatcaatgt cgtagattta
    17281 ttgaaattac gtcatccaag tcaagatccg cgtgggttga gcgatgaaga atttgatcaa
    17341 tattttacaa aagacaaacc gatcttattt gctttccacg gttatgaaac attggtccgc
    17401 acgattttct ttgatcgtca taaccatcat ttaatgattc atggctacaa agaaaatgga
    17461 gatatcacga caccatttga catgcgtgta gtaaatgaac ttgatcgtta tcatttggca
    17521 aaagacgcag ctttaaaaat caaaggaagt caagcagaag attttgcgga aaaaatggat
    17581 cagaaattac aagaacatca aaactatatt cgtgaaaatg gtattgatct gccagaagta
    17641 ttggattgga aatggaaaaa tcttgaccaa taaaatgata aaatcgatcc cgcaaaacag
    17701 aattttttac attgtattat ttgtcaaatt aag
//
LOCUS       DAYYME010000053        17647 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000053 DAYYME010000000
VERSION     DAYYME010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.053"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..899
                     /locus_tag="ACHBFW_001465"
     CDS             24..899
                     /locus_tag="ACHBFW_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFM9317775.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1044..1196)
                     /locus_tag="ACHBFW_001466"
                     /pseudo
     CDS             complement(1044..1196)
                     /locus_tag="ACHBFW_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1464..2795)
                     /locus_tag="ACHBFW_001467"
     CDS             complement(1464..2795)
                     /locus_tag="ACHBFW_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9317776.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(2944..3657)
                     /locus_tag="ACHBFW_001468"
     CDS             complement(2944..3657)
                     /locus_tag="ACHBFW_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9317777.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            3832..5256
                     /locus_tag="ACHBFW_001469"
     CDS             3832..5256
                     /locus_tag="ACHBFW_001469"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9317778.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5555..6262
                     /locus_tag="ACHBFW_001470"
     CDS             5555..6262
                     /locus_tag="ACHBFW_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM9317779.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6249..6572
                     /locus_tag="ACHBFW_001471"
     CDS             6249..6572
                     /locus_tag="ACHBFW_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM9317780.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6661..7698)
                     /locus_tag="ACHBFW_001472"
     CDS             complement(6661..7698)
                     /locus_tag="ACHBFW_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM9317781.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8019..9002)
                     /locus_tag="ACHBFW_001473"
     CDS             complement(8019..9002)
                     /locus_tag="ACHBFW_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFM9317782.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9417..10187)
                     /locus_tag="ACHBFW_001474"
     CDS             complement(9417..10187)
                     /locus_tag="ACHBFW_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9317783.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10180..11115)
                     /locus_tag="ACHBFW_001475"
     CDS             complement(10180..11115)
                     /locus_tag="ACHBFW_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9317784.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11431..13080)
                     /locus_tag="ACHBFW_001476"
     CDS             complement(11431..13080)
                     /locus_tag="ACHBFW_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM9317785.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13197..15800)
                     /locus_tag="ACHBFW_001477"
     CDS             complement(13197..15800)
                     /locus_tag="ACHBFW_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM9317786.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15812..16861)
                     /locus_tag="ACHBFW_001478"
     CDS             complement(15812..16861)
                     /locus_tag="ACHBFW_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM9317787.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(16887..17201)
                     /locus_tag="ACHBFW_001479"
     CDS             complement(16887..17201)
                     /locus_tag="ACHBFW_001479"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFM9317788.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17217..>17647)
                     /locus_tag="ACHBFW_001480"
     CDS             complement(17217..>17647)
                     /locus_tag="ACHBFW_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9317789.1"
                     /translation="NINNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQKDVFDR
                     ENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLLAIKDTD
                     KGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
BASE COUNT         5386 a         3586 c         2939 g         5736 t
ORIGIN      
        1 gtgtttcatg aaaggagaac tagatgaata attggcttga gtattttcct gaaaacgttt
       61 tggaacgcgg ttacagctat catctgcacg gttttgtccg tcatttgaac tatacaagca
      121 agtacttgtc cgcaacagtt tctggcacgg aagattataa ggtagtgatc acttgggatg
      181 aaaagaccaa catgacttgt gattgtctat atgccataga aggaaaaaaa tgcaaacaca
      241 tggctgctgt cttattcgct tacgaagaac gtccaataaa aaaatccaac tactcacttt
      301 ccgaactctc gtcccttgtt tcctccgcta gttcctctct tgtacgggag cttttaacag
      361 agattcttat agaacatccg caatttatcg aacgcttcaa agttaagatg ccatttcatg
      421 caataaatta ttcagataaa ttaacaacta tcatccataa atacgatcat attatcaaga
      481 aaaacaaaaa caggaaaaca gcgaagttca tcatggagat gcgaaagttc atccaagaag
      541 cagttgaatc gctcattcag caaaatgcct atcttcccgc attcgaattg atcaatgaag
      601 tgattgctac tctcgaaaca ttctattggg agcctgagga tgaacgaacc cttcttctga
      661 tagaggactg ttattattta tggaaagaat tattagcaga agcacctcat gcagaaaaaa
      721 ggcaaatgtt ctcttggttc gtctgccaag tcgatcatac tgacgcctct tattcaaaac
      781 gctactccat aaaaatcctg aaagaagact ttcgggaaaa agaattttct aaccaaaaga
      841 aaaaaataga taaaaaaact aaagaaacgg taaagaaaga cgatttcaat gaaaaatagc
      901 aagtaaacga taacaaccat cctaaatcca caagaaatac agcagacctt gctttaaaaa
      961 cacgaatcat gcaaaggatg aagtaaagaa gacttaacgc gtacccaata ttagtcattt
     1021 gactattagg tacgcgtttt gttttataag tcatgacaaa tttccttcgt tgctgggtag
     1081 attttccggc attgttgata gattttatga tacttttcta cattttgtaa gagaggttcc
     1141 actgcatctg tatattggat aaaattctga caacaacttt caggtgtgtc gaaccactct
     1201 aatctttaaa gataaatatt aaaataacga ttaataatat taacagtctt tatttttcac
     1261 caccaaaaac ctatctttgc atatactaaa tagacgtata tacacagata ggtttgtctt
     1321 gtgaaaaata atgattatcc ttaccaaaaa atgtttgttt ttctacatgt tcgtacttat
     1381 caaaaaatat gatccttatt gaaattactg gctatttata ttccaacgac ttcttgtgtt
     1441 aatttttgtt atctcatagt aaattatgtt tcattttcac ttaatttagt tgtcacgtgt
     1501 tcgtcaattc gtaaaaacgg taaatatata aacacacttg cggctataat taatatttgt
     1561 aaaacagctg ctcgccagtc tccggctgtt gctaaaaatc ccgatataac tggtggcgta
     1621 gtccaaggaa tttgcactac tacatgatta attaatccca cagcagttgc aaaatatgct
     1681 actgtcaaag aaaaaatcgg tgcaagcaca aagggaataa tcagactgat attaaataca
     1741 ataggcaatc caaaaataat aggttcgctt atattgaata tagcgggagt taccgataat
     1801 ttagcaaagt ccttataatc ttttctacga ctgaagataa atattgcaat caataaagca
     1861 atcgtatttc ccgatccacc cattaccgca aaggtatctt taaaggccat attgataata
     1921 tgtggaattt gctctttgtt tgcataagct gccatatttt cttgcatatt ctgaatcaaa
     1981 aacggatcta acaatgaacc tgatattact gcttgatgga taccaaatcc aaaaaataga
     2041 ttagcaatag atgtaattaa caaaaatcct ggtaaaccag ttgtaatttt acttaatggc
     2101 tctgtaacta tcgtggtaat tagcttattt atatccatat taaacaattg aatgattata
     2161 aatgacaata aagcaaacga ggtaatcgta ataatgattg ggattaatac attaaaagac
     2221 tttaaaacag cgggaggaat atttccggat agctgaatct gtagtttctt attattagaa
     2281 agtttaataa ataattctgt agccgctaat ccaacaaata tacctacaaa cattcctgaa
     2341 gctcctgtat aggatatagg tatcacaccc gtcacttcaa cagctttatc cgcattttca
     2401 ggagtaaaca tcatctgcat aggtgttaat acaacaaatg tcgaaatagc aactaatacc
     2461 gctgcaatat gatttgtata atttctgctt gcacacatat gataagatac tgctgctaca
     2521 actaataatg tagttatgct taatgtacca ttggcaattg aagtaccaat ttgttgtaac
     2581 gtggtcaatg tactagtatc gacaatttcc cccatgaatc ctgttggttc taagatcact
     2641 gaattaataa gtgtcacaaa tcctgctaac atcataaatg gcataaatgt agcaaacgca
     2701 tctcgcaaag ttcttaaatg gatctgattc cccaacttag tcgcaactat accaaatttt
     2761 tcaaatacaa tgtccttcct gctacttttg cccatatttt ttctccttta tgcttattgt
     2821 aatcgattac tttttccacc caaataatac atcacttatc aaaaaaagta aatactttta
     2881 attaaatata tatatctttt ttagtataaa aaaataagct ctgaaaaaat aattttcccg
     2941 agcttatttt tcaacaaaaa cagcttcaaa aacaaagtct tgatataaat gtctcgatac
     3001 cgaatactcg aaaggtttac cagacgatag agcaacaact ttgtctatct caatcataaa
     3061 ttcacctgga tctattttta atatttttgc gtcaaattca ttcgcagtat ctcctttgat
     3121 acgtatgtgc gaagtcttta tttccagacc cagttcattt gtgatataag catatacaga
     3181 tttctttaaa tgttttggtt ccaagcctgg tatgactgct aatggcatga atgtttgctc
     3241 aatcgagtat ggttgacaat caataaaacg gcttcgtaca atattatata catagtcttt
     3301 tacactgata cttaatttat cagctacttt tttcgatgga agttcaattg agaaagtatg
     3361 gatttctgaa tctacgtgtt cttcaccaac cacgctaaac aaaccttcgt ttggaccaag
     3421 aactcgtgag ttatcaattg gtgttctgac gacttcagtc ccatacccac ttctacttcg
     3481 caaaactcct tcggaaacaa gtaagtctac gcctttcttt actgttaact tgcttgtatg
     3541 ataaataact gccaattcat ctcctgtagg aagtttctca ccaattttat actctccatt
     3601 tacaattttt ttctttatat ctaagtaaat ttccatatac ttcgcaattt ttttcaatgg
     3661 tgaccctcca atcaaaaaca tatcttcatc tatactttaa cataaataaa ccgataaaat
     3721 ccacatcaaa attatacaca caataaaaaa gatatatata tttaaacaaa actatttact
     3781 ttaagttgta agcggtgtta tcattaagtg gaaacaggag gtaaaaaaat tatgacaact
     3841 atgaaaaagc taacttttcc agatgatttt tggtggggtt ccgcttggtc agctgagcaa
     3901 gcagaaggaa aaggagaaac aggaaaagca gagactatct gggatcgctg gtttaaagaa
     3961 caaccttacc ggttttatca acgaatcgat tcctctatta caaccgatca catccatcaa
     4021 tataaagaag atatagaact aatgaaaaaa actgggcata actcctttcg cgtttctatt
     4081 tcttgggcac gaatgtttcc aaatggcggt accggaaaag taaatcccaa agccatttct
     4141 ttctatcgaa aattattttc cgaaatgaat aataacggaa tcaaagtatt tgccaactta
     4201 tatcattttg acatgcctgc tactcttcaa gataaaggag gatgggaatc acgtgaagta
     4261 gttgatgctt atgttcatta cgctgaaact tgttttaaag aatttggtga cttagtttgt
     4321 cattggttta ccttcaatga accacttggg cctattttag gtacttatct tgaagatttt
     4381 cattatccaa acctcattga ctttaaacgc ggagcgcaag cagcattctt cactatactt
     4441 gcacacgcaa aagcaataca agtatttaaa gaactagctc ttcctagtaa aataggcgtt
     4501 attctaaatt tgagtcctac ttttcctcgt agtcaaaatc ctgctgatgt gaaagcagca
     4561 gaaatcgctg atttatttta tacgcggagc tttttagatc ctatggttaa agggaatttt
     4621 tctaaaaagc ttatcctgtt attacaagaa tataatcagc ttcctgaaga ttatacgaaa
     4681 gaagatcttg tatgtatcta cgaaaatacc gctcaaattc ttggactaaa ctattacgag
     4741 ccacgtcgcg tcaaatctcg ttcttctatg gtggatacat ctagtccatt tttaccagag
     4801 tggtttttcg aaccacatat catgcctgga cgacgaatga acgaataccg gggatgggaa
     4861 atttacgaaa aaggaattta tgatttgtgc atggatatca agaaccatta tggaaacatc
     4921 gaagcattta tctcggagaa cggaatgggc gttgcggatg aagaacgttt catggatgat
     4981 acagggcaaa taattgatga ttatcggatt gagtatatca aagatcatct tgcttactta
     5041 tggaaggcaa taaatgaagg atgtaacatt aaggggtatc atctttggac atttattgat
     5101 tgctggtcat ggatcaacgc ctataaaaac cgatatgggc tggtttcact cgaccttact
     5161 actcaaaaaa gaacaattaa aaaaagtggc gaattttaca agcaattaag tgatacaaat
     5221 ggatttagat acgatgtaga cttgttagtt gaataatatt ctaaaattcc acttgaagtg
     5281 aacacattag aaaacaagaa taatgagaaa taccaataac aaatactttt tcttcatact
     5341 tcatttatac ttgtcagtat aagagactgt gacaaatgta gtgatcctca gaagttaggt
     5401 ctggaaatct aacttgagaa gattttttta aactacgtcc agtctttttt atttgtttaa
     5461 aattgaagtt gacaagtctt cattaaaaat ttagaatatt cacaagtttg acgacaatta
     5521 tgaaaattta tgtcgtgcta aaggagtttt gttattggat gaaaagttag atataaaaac
     5581 tgcgatcaaa gacacgttac ctaccgtttt tggttatatc ggtattggac ttgcatttgg
     5641 tatcgttggg aaagctgccg ggtttcatcc gctggtcgtc acgttgatgt ccctgctgat
     5701 ctatgctggt tctgcccaat ttatcacggt cagcatgctt gctagccata gtccactgct
     5761 ctccatcgtt ctctcgacct ttctagtcaa ttcccgaatg attctgatga gtatgacgat
     5821 tgctccttat ttcaagaaaa atcggctgct tcaaaatctg ctgattggca cattgctgac
     5881 agatgaaagt ttcgcactgg gaatgaataa attgaactat actggacaaa agttaaactt
     5941 tcgttggatg aatacagcta attggatttc ttatttgacg tgggtgggtt cttctcttgt
     6001 tggcgcactt ttagggaatt ttattactga tccgaaaaag tttggtttgg atttcgccat
     6061 cgtcgctatg tttatcgggt tactttatct tcaagtcatt tctgatagaa acacctctaa
     6121 acgattacag ttgatcctga ttggtctcac tttgattctg gtatatgtcg gactgatttt
     6181 tatcccaagt aacttagtga ttttagttgt aacactgatt gggtgcggat tgggggtgtg
     6241 gatcaaacat gccttctttt gagtttacat tgctgactat tttaggttgt actttagcaa
     6301 cttggctttc taggatccta ccatttgtct tactgaaaaa attcgatttg ccgcaaccgt
     6361 tgcttgagta tttaagtttt gtcccgatcg tcattatgtc tgctttatgg ttcagcagtc
     6421 tatttaccca aaatatcgga catctgccgc agatcaatct cgaaaatgct ttggcttcag
     6481 ttcctacact tttagctgct attctttcaa aaagcttatt ggtgatcgtc ttggtaggaa
     6541 ttctttccct ttcgttgatt cgcttattat aaataaaaaa acaagagctt atggcaaatt
     6601 actgtcatgc gctcttgttt ttatcaaagt caaataagaa aaaaatcggc gtattcttgc
     6661 ttatccgttg aacggaccac taacaacgac ttttcctaat atatgtcctg tttctacgtc
     6721 tttggttgct tgtttcaagt tcttcgcatt catcccatct gaatagacct tgctcaatgt
     6781 actgcggata atcccttcat ttgctaaagc agcaatgtgc gctaaagctt ctccttgcgt
     6841 ttcgatttca tagttatagt ctgttttagc gaacatatac tcccagtcga agctgataga
     6901 ttggtttttc caaacaccta tatctaacgg ctctttgatc cctacgatcg ttcccacatg
     6961 tccaaatggt cggacaaggt gtttgatttg atcaaagtag cgggtaatat cgaataatac
     7021 agcgacatac tctacttgat cgattcctaa ttcacgaaaa cttttactta agtcgtgatg
     7081 ataatcgatt ggatagtcta cacctgtttt tttcagccat tcaaaattct tcggacttgc
     7141 agtcgcgtag acttctaggc cagcccaatg cgctagttga ttcaaaatcg aaccgacgcc
     7201 cccagctccg ttgatcacta agattttttt cccgcggttg gcattttctt ctggagtcag
     7261 accaaatttt tcaaataaca attcataagc ggtcaatgag gtcaacggca aggctgcggc
     7321 ttcttcatct gaaagatttt ttggtgcaag agcgacaata cgttcgtcaa ccagttggta
     7381 ttcttgattg cttcctgctc ttgtcgttgt acctgcgtaa aaaacgcgat cgccgacaga
     7441 aaatttttgc acttcatcac ctacagcaac aactttcccg actccatcaa accctaagat
     7501 acgtaaagca tttcttatcc cgttatcttg gcgaagtttc gtatcgacag gattaacaga
     7561 tactgcgttg acttttacca gaagatcccg tgaactgggt acaggtatct gttctttttc
     7621 atcaataaag ctttttggat catcgatcga caacccttca taaaaaccga ctaccttcat
     7681 taattccttt tctgacatac gaattcctcc ttagcttcat caagttggag catccataca
     7741 gttttcattt caatatatat agtatgctat ttgttccact tttattcatc ataattattc
     7801 tcttacttaa agtaaactat ttgcactttt ttggttaaaa aagaggttgc gacaagactt
     7861 ttgtcacaac ctcttttaaa cggtgttcaa tcaactgacc ctcggtattc gctcaaaaaa
     7921 cgacaaaata tgaggagcta tttttgtttg gctgttctta ttacttgatg caggacagct
     7981 ttttcacgac ctctaattca cggtgttcaa gcaagaaatc attgttcact gaaaagcgtt
     8041 ggatgttctt tttcaaaggt gtctaagatc tctcgtactt catcagtaga tttcgtattc
     8101 atcaactgaa ctcgcaagtc atttgctcct ggaaatcctt tgatatagat tttaaagaaa
     8161 cgatgtagtc ctgtgatgga acgcgggatg atttctgaat aatgatcttg cagatctagt
     8221 tgcagacgaa gtagtccgat cagttctttt tctgtatgtt ccctcggttc tttttcaaat
     8281 gcataaggat tcttgaaaat cccccggcca atcatgatgc catctacgcc atatttttcg
     8341 gccaattctt ctcctgtctg gcgatctggg atatctccgt tgatcgtgat cagtgtttgc
     8401 ggagcgattt ggtcacgtaa agcaatgatt ttagggatga cttcccaatg tgcatctact
     8461 ttactcatct ctttacgcgt acgcaaatgg acagaaagat tggctatatc ttgatgaagt
     8521 aggtgactga tccattcttc catctcagac atttccgtat agccgatacg cgtcttgacg
     8581 ctgacaggca aaccgccagc tttagccgct tcaatcaatt gtgccgctac ttctgggcga
     8641 aggatcaagc cactgccttt tccacgttct gctacattag ggactgggca gcccatgttg
     8701 atatcgattc cttggaagcc catttctgcc atagcaatgc tcatttctct aaaaaattct
     8761 ggcttgtctc cccaaatatg ggcaaccatc ggctgttcat cttctgtaaa ggtcaatctt
     8821 cctcgtacac tgtcttttcc atctggatga cagaaactgt ctgagtttgt aaactcagta
     8881 aagaagacat ccggtgctgc tgcatgcttt acgacatggc gaaatacaac atctgttaca
     8941 tcttccattg gtgctaaaac aaaaaaaggc tttggcagct ctttccaaaa attagtagtc
     9001 atttatttgt tactctccat tctatcaagc agtgaagtcg caatctttct caaaaacatt
     9061 gtcattatac taggaaacaa aaaaagaagc aaagtgaaca gcttaaaaga tgtttctgat
     9121 tgagcaaaaa aagactgtga caaaaatctt atcacaattc cttttccgga ataaatggtg
     9181 ttcaaaagct tgcatctgcg gtaataagtc caaccaaacg aaaaatagga ggagctattt
     9241 ttgtttggct gttcttatta cttgatgcag aacagctttt tcacaacctc tccactaaac
     9301 ggtgttcaat cagctgaccc tcggtattag ctcaacaaac gacaaaatat gaggaactat
     9361 tttcccgttt gtttctctaa tactcaggtc aaaacgctga tttcacaacc tcttttctaa
     9421 cttctcaatc ctcttccttt attgatcaaa taccaagtaa cggcgtacaa aacaacgatg
     9481 aatagcacaa gtacgatcat cgatgttgct actggcacat cgattgttcc aaggaatccg
     9541 taacggaaac cggagatcat ataaacgatc ggattgactt tcgaaacagc ttgccaaaat
     9601 ggcggcaaca tagagatggc atagaaaacg ccacctagat aagttaacgg ctgcaagaca
     9661 aatgtaggca cgatcgatac atcgtcataa gactgagcaa aaatcccgtt gagcagtcct
     9721 gctaacgaaa acaagattgc cgtcatcaat aatgtaatga tgaccatgaa ccatgaatac
     9781 acatgaagtg gtacaaagaa caaggaaatg atcgttacga gagagccgac gagaatactg
     9841 cgacccactc cgccaatcac aaagccccaa atgatcacat gcgtaggaac aggcgctacc
     9901 aagatttctt cgatgttttt ctggaatttt tgagagaaga aggacgaaga aacgttggca
     9961 tacgaactag tgatagcaga catcataatc aaaccaggca cgatgaattc catatatgaa
    10021 aaaccaccca tgtcaccgat ccgaccaccg atcatttttc cgaaaataac aaagtacaag
    10081 gatgtcgtga tcacaggcgg aaccagtgtt tggacccaga tacgcaaata gcggttcgtt
    10141 tccttgatag ctaaactttt taatgcagta aaataaagat taaacatttt tctcctccgt
    10201 atgattattt tcttctgtga ttttcaaaaa cagttcttct aaacgattcg acttattcct
    10261 catagaaaga actttgattc cttgtcttgt cagctgatcg aagattccgt tcaccccttg
    10321 atttcgttca acttcgacag cgattgtctg ttcatcttca aatgtacaag cgtatccttc
    10381 aatcacaggt ttggtctcat atggcgctaa atcaaagata aatgtttcaa attgcagttt
    10441 ggatagaagc tctttcatac tagtattctc gatcagttca cctgattgga tgatcccgat
    10501 gttgcgacaa agcatttcag cttcttctaa ataatgcgtc gtcaaaatga tcgttgtacc
    10561 attttcgttc aattctcgga gaaactccca catctcgcgt ctcaattcaa tatcgactcc
    10621 tgctgttggt tcatccaaga tcagcaaacg tggttcatgc atcaatgccc tagcaatcat
    10681 cagtcttcgc ttcattcccc cggaaagcat acgtgcacgg acatttctct tttcccaaag
    10741 attggactgc ttcaaatatt tttcgctgcg tttcatcgct tctttgcgag agacaccgta
    10801 gtagcctgct tgattcacga cgatttgctg gaccgtttca aagggattga aattgaactc
    10861 ttgcgggact aatccaatct gttgctttgc tcggactaga tccgtatcca gatcataacc
    10921 gaagacattc acttttccag atgttttatt gactaaagaa gtaatgattc cgatcgttgt
    10981 agacttccca gctccatttg gtcctaatag cgcatagaaa tctccttctt tcacggaaag
    11041 atcgattcct cgcaatgctt cgaccccact tccataaacc tttttcaaat tatttatttc
    11101 aagcgcattt gccatttatt tcctatctcc ttatagacat gattctttat tgtatcatgg
    11161 aatacagcag atgaaaaaac aacttggttc cagcgaaaaa tcaggttgct gggctcagga
    11221 aaatctccta atgaaagaca tcaatcggac gaagtcagac aaccttttca gaaaaaacaa
    11281 atcgaagata aaggattact attcatatgt gcaacaaaag gagcgtgaga caaaagtaaa
    11341 gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcaaaag ccaagatttt
    11401 tgatccaata aatactttcg atcacttcgc ctatcccaat acatgccact gccgcttttc
    11461 atcaaataac gcggcttccg tttttgcggt ttctactaaa gtctctgggt aatccaaaac
    11521 tttcagcaag gcaatcgcat ttcgggtcct tgaaggtcct tcttttaatt tgaaatcaaa
    11581 actgattcct tttccttctg ttacttgttc ttcaaagtgg acattctcac aataattctt
    11641 taaaatctct gtcaattcga tatcatgtgt ggcgacaaat gctaaagatg gatactccgc
    11701 aagccaccga acgactgaag cggaagaagc aatccgttcg atcgtatttg tcccttttaa
    11761 aatctcatct acgaagcagt agcagcgttc tttggaagcc acttggtcca acaaacgttt
    11821 gatcgatttg atttctgaaa caaaataact gtcgccttca aataaatcat cttcgattgc
    11881 catggaagtc aacacatgtc caggttgcag ggtaaactgt tctgcaatcg ctgtttggat
    11941 cgtctgtgct agaatacagc tgatggctat acttttgaca tacgtagatt ttcctgatgc
    12001 attggaaccc gtgactaatg tattcttctg ccagttgacc ggattcacta ccgcttcttt
    12061 taacaatgga tgatagatat ctttcgctgt cacaccgccc tcagtaaact ctggtcggca
    12121 agtgatgggc atataagtac ggtaattcaa tattgcagcg gctacttcta attctcccag
    12181 caattcccac agcttgatgg cttcagttga atgcttttct aatttagtga gaacgaaatg
    12241 ataggagata aatggcagca tgaacatgat gttgacatat tcaaaaatca actctgcttc
    12301 actcccactc ttcatacgaa atgaaaaacc taatttaggg atgtctctta atggtttagt
    12361 ttgctgtttc agctcttctt gaagtggtgt cggcagtttt gctagctgat tggcggctga
    12421 aatcgttgaa accaaataac tcatgctatt cagctctgtt tctagtgttt gtttcttcac
    12481 tagataatag accgtattga atactaaccc accgattgcc aaatagattc ctagtacttg
    12541 tcctaaaaga agcaaaccca aacccaccag cggaagcaag ccaagaaaaa taaataaacc
    12601 tacgcgtcca atctttttac tttttccatc cgctagatat tgttttgcaa aattatgatc
    12661 tttttttcct aaacgagcga attgatattg gattttttct ctcagttgag gattttcctg
    12721 ataaaaagca atcagttttt ctaactgctg atcttcacca aaatcaaagc tgcgtaaacg
    12781 ttgatacaac gcttctgaac ctacactaga ataggtactg ttgattcctt cgaatacttc
    12841 gaacatatcc aggtcatacc aagtaagatc atcgatttcg ctatcccatg aacggaactt
    12901 cttctccgtg agccatgctt ccttcaagct ttcttcttta tcaaatctcg gttgataagg
    12961 aattttttcc catttgctac gaactgtttc ctttaatttc aaacgtgaaa aaatgtctaa
    13021 agcaactaat aaaataacag ctgcaataat aattaaaacg ataaattctc tttgacccat
    13081 atccctctcc gttcctttat ttgttagctt tagtatagca ctgttgcgct atttgaaaag
    13141 agggtaaaaa aacacctttc aaaataaaag gtgttttttt cttgtatatt acatatttat
    13201 ctctcattta tcaaaattga caaataatca tagggaggaa ttgttgttaa ttcttgttta
    13261 ttctccagtg cattgacaac tgtcccaagg gaaaggatag cctcaggtac tattttttct
    13321 tgttctgttg gattagctaa gcgaagaaca aatccttgtt catgaaaata agaaggataa
    13381 atcgatgata caattaagtc ttcaggtaat tcaatcaatg aaaatttacg aggaacatca
    13441 agttcatttt gtaatggcca aatcttgtta tctaacctgt gaataaatac attcagtttc
    13501 tgcttttgat aagaaataga ttgttctaac cgtttttctg cctgttttgc cactttgaac
    13561 tcattaaatc ttccttcttc tacattaaat ccaaaagtga ctttatactc acctatctct
    13621 tgagctagcg gtgtttccat catgacatgc ccttcatttg tcgtatctcc tgaagctcgc
    13681 ccaggtcgcc aagcaagatt aggttttcca agttcccctg ttgttgaaaa taaggttatg
    13741 attaattgat tttctatacg ttcatactct tttaaaccat ctgcataaaa cgtaaaataa
    13801 tgatcatttt ctgccacaga gacactctta tcgaatattt ctaaattaac aggtttctct
    13861 accatagttt ctgaccaatt cggaggaatg acatcagggg tattttcaat aaatccgttt
    13921 tgaatttgtg caattgattt ttcagattta tctaaaacat taattgctaa gcgcatacga
    13981 tgcgagtaaa cagtattatg aattttgagt gtgccttcaa cgatttgact ttctcccaga
    14041 ctgaacgata tttcataaga tatttcttcc atttcgggtt tttcagataa acgatctttt
    14101 aaatctttag gaagttgagc ttttccgtaa acaactaatg tttcttgtaa agaatcttta
    14161 tatactttag ctgtttctaa ttttagagat agttctgtat ctccttctaa tggtgaataa
    14221 tcatacgtat cgccatcatt tgctgaatcg atcaaatgaa cgaaatcaag atattgtcta
    14281 tctccaacta tcaaattcaa ttgcccatct ttaaaagaaa gtttatattt attgttactt
    14341 atctcagttt tgtctatctc gattgctgtt tctaattttt cattattttt ctcaaattca
    14401 acaacagtgt atcctagtgc tggcacattt acatttattt ttatgtcaag ttcataataa
    14461 gctggttcat caaaatattc gaaacctgta gcaatcatcc gataagcatt tttccgttct
    14521 ggaaaataaa cttctttttc aatgatatta tagggactac ctttgaagtg tattttcttt
    14581 gaatcagcta ctacatgcac gattttcgat ccactataat tctcagtcgt tgtgttgaaa
    14641 acaaggactt cattcgattt tagctttaat gcatcagcca tccgttttac aataaggttt
    14701 tcgattccgt cagctatctc attagcttct ttgattcgat ggaaaatatc ttccgcaaca
    14761 ttatctgaaa cacagccacc aatactatca tgagcctggt tttgcaaaat ctttttccat
    14821 aaccgacgaa gtaggccttt actgatatgg ataccatttc tttctgcaat cagcatgagc
    14881 ggctcaactc gttttagaat tttttgttcc aatttaaaat tttctatttt catttttgtt
    14941 cttactgagc caatagaacg atgaacacga gagtaaaccg gctgacgaag ttcaccttta
    15001 tattcaggta attctttttt gtttctgata acttctacat aagaaggata atctgaaata
    15061 atattgtgga atttactgta tttattcaac ttctctacga cttgatcaaa atttaaaatc
    15121 attgactttt gatctacacc cgaaggtact aaaatatctt tgttttttcc ttgcatatga
    15181 agcatttcaa ctgcttcatc caacttttct tttgcaaatt caggaatatc tttaattgcg
    15241 tctaaggtca tcattccagt catataacca aatggaaaat tcatggcata aacaaattta
    15301 tctcccaatc ctttccagat aaaataagga gatggaacca tcgtttcaaa gtttaatcct
    15361 cgccaacaca tgaaattatc gatttctact tgatgcaaaa gtgtaggcaa attcgcattg
    15421 aacccaaacg tgtcaggcaa ataccctacc atcattggat ctccatattt aaggatcgta
    15481 tcactgattc caatcattaa gtttcttaaa attgattcag catccactaa taaggcatct
    15541 gtttgtgcat accacggtcc cacgaatagt tgtcctgttt tcatcagtcg ttggataacc
    15601 ttaagctttt gcggattgat ctccaaatac tcatcgacga ttgaagtttg tccatctaaa
    15661 caaaagttta ctttagggtt cctttccaat tcatctagaa cttcagtaaa gacctgatca
    15721 gacaatacga tggcatcttc atttgtaaaa taccattctc tgtcccagtg cgtgtgttga
    15781 attatatgtg catttaccat atcagattct cctactttcc ttcttctact gaatttactt
    15841 gttgttcttt tttatacttg atggatttta gtgcattgac cattaacgct gtaaccactc
    15901 ccccaataac aattgatact aaatataaag gtatattcgt aacaaaagga attgccataa
    15961 aggttgaaag cattgtttct tgagtaacac caaaaaatcc atttagacca catgctactg
    16021 cagtgcccac cacgatgctt ggtattactc gtaaaggatc tttcacggca aacgggatcg
    16081 tcccttcagt aatccccaca aggcccataa tcatagctgt ctttgcatta tctcgttccg
    16141 attcatcata aagttttttg tttaaaattg ttgctaaaaa cattccaaat ggcggaatag
    16201 caattcctaa taacatggct gtactaggtc caaagcttcc gcttgaaaaa gaagcaaccc
    16261 caaaagcata tgcgatttta ttgataggtc ctcccatatc aaacgccatc attcctccta
    16321 aaatagccga catcaaagca gcatttgctc ctgacatgcc ttctagccaa ttagtcagtc
    16381 ctgttgtcat tgcttgaata ggcgtagcta gtacatacca taatatgatt ccaacaacta
    16441 atgtagtaac aaaaggaatc agcataattg attttagact aataacttgc atcggtactt
    16501 ttactttttt caatgcatta gctaaatagc cacaagctaa tccagcaacc attcctccaa
    16561 taaatcccat accaagattt tgacacatca tcccagtgat aaaagctggt gctagaccgg
    16621 gacggtcagc aattgaatat gcgatatagg ctgaaatcat cggtacgata aactgtaatc
    16681 ccgtctgacc aatcattcgc aggactcccc agaatgtatc cgtctggtcc caaacacctg
    16741 tgccaccaaa aatgactgca atagaagtta gtaaccctgc tgctacgaca agcgggatca
    16801 tataggaaac cccactttgg aaatgattta gaatttctct tccttgtttc ttgaattcca
    16861 ttagtaactc ccccacgata tattttttat ttgttaatta atgcaattgc ttcatcaatc
    16921 gtctcttcac catcttttac tacctctgag gtccctactg ttaaaatcgg tttaccgtca
    16981 aagcgacctc gttctgaaac atttgtatct actgcaaata tgacaacatc tgcctcggaa
    17041 acatccgaaa ttgaaagttt gttttctatc cccatagccc cctgtgtttc tactttaata
    17101 tctaaccctt tcttcttcgc aaacttttct aaattagctt ttgccatata agtatgggca
    17161 atccctgcga tacatgctgt tactgctact atttttgtca tatgaaattc ctccttttaa
    17221 aatgatagag cttttaccaa ttctttttta tttctcgaat cttttattct tttaacaaac
    17281 tcgtcatcca tcaatcttcc agataattct gctaaaatac gtaaatgatt agtaccttta
    17341 tcagtgtctt ttattgctaa caaaaagacg agatcaactg gttgatcatc taatgcgccc
    17401 cattcaacag accgctttaa cttggctaca gccactgttg attcaattac tgctaggctt
    17461 ttgccatgag gaatggcgat atttttccca attcctgtgg tcatatggtt ttctctgtca
    17521 aatacgtctt tttgaaattg tttgtggtca gacacaaacc cattagtctc taacaatttc
    17581 gataattttt cgactacact ttttttatca gatgcattta aatctatcac tatattattt
    17641 atattga
//
LOCUS       DAYYME010000054        17632 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000054 DAYYME010000000
VERSION     DAYYME010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.054"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACHBFW_001481"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACHBFW_001481"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFM9317790.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="ACHBFW_001482"
     CDS             complement(2260..2511)
                     /locus_tag="ACHBFW_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFM9317791.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="ACHBFW_001483"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="ACHBFW_001483"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFM9317792.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="ACHBFW_001484"
     CDS             3461..3679
                     /locus_tag="ACHBFW_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317793.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="ACHBFW_001485"
     CDS             complement(3968..4480)
                     /locus_tag="ACHBFW_001485"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFM9317794.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="ACHBFW_001486"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="ACHBFW_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFM9317795.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7376)
                     /locus_tag="ACHBFW_001487"
     CDS             complement(6933..7376)
                     /locus_tag="ACHBFW_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFM9317796.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="ACHBFW_001488"
     CDS             complement(7377..8165)
                     /locus_tag="ACHBFW_001488"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM9317797.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /gene="rnz"
                     /locus_tag="ACHBFW_001489"
     CDS             complement(8162..9103)
                     /gene="rnz"
                     /locus_tag="ACHBFW_001489"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFM9317798.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="ACHBFW_001490"
     CDS             complement(9280..9867)
                     /locus_tag="ACHBFW_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFM9317799.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="ACHBFW_001491"
     CDS             complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="ACHBFW_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFM9317800.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="ACHBFW_001492"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="ACHBFW_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM9317801.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="ACHBFW_001493"
     CDS             complement(12784..13719)
                     /locus_tag="ACHBFW_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9317802.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13905..14864
                     /locus_tag="ACHBFW_001494"
     CDS             13905..14864
                     /locus_tag="ACHBFW_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFM9317803.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(14950..16044)
                     /locus_tag="ACHBFW_001495"
     CDS             complement(14950..16044)
                     /locus_tag="ACHBFW_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFM9317804.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            16233..16994
                     /locus_tag="ACHBFW_001496"
     CDS             16233..16994
                     /locus_tag="ACHBFW_001496"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9317805.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(17109..17549)
                     /gene="fabZ"
                     /locus_tag="ACHBFW_001497"
     CDS             complement(17109..17549)
                     /gene="fabZ"
                     /locus_tag="ACHBFW_001497"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM9317806.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT         5069 a         3838 c         3087 g         5638 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttagt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataacccg cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc
    13861 aaattgtatt tccgacgaaa gaaaacaaag gagtgtaacc ataaatgacc tacaaacatc
    13921 ttaccataga cgaactgaca atgatagaat catattatct tcaacataat aagccggttg
    13981 aaatcgctaa ccgaatggga cgtgctatac aaactattta taatgtagtc aataagttca
    14041 agcaaggcaa gactgctctt gattattggc accagtataa agaaaataag aaaaaatgtg
    14101 gtagaaaagt cattcaatta cctgctcatg aagtagatta cattaaagag aaagtcactc
    14161 ttggttggac gcctgacgtc attatcgggc gaaaagaaag gtctgtttca tgcggtatga
    14221 gaacacttta tcgtttattt tctaaaggaa tatttgatat tgacacacta ccgatgaaag
    14281 gtaaaagaaa acccaatggc catcaggaaa aacggggaaa acaacagtat cagcgctcaa
    14341 tccatgatcg acctgataat tatcctgatt tcaattctga gtttggtcac cttgaaggtg
    14401 atacgatcgt tggcattcat cataaaagtg ccgtcattac tttagttgaa agattatcta
    14461 aagtcattat cacgattaaa cccaacggcc gtaaggcatt agatattgaa actgccctta
    14521 atcaatggtt ttctcgcttc cctaaaaact tctttaaatc tattacgttt gactgtggaa
    14581 aagaattttc taactggaaa gccattagta accaacatga tattgatata tattttgcgg
    14641 accctggaac accttctcaa cgcccattaa acgagaattc taacgggatt ctgcgtcgta
    14701 atggactgcc gaaatcaatg gattttagaa aagtgaatca gacatttatt tccagtgtca
    14761 gcaatcaacg taatcatatt ccaagaaaat cattgaatta cagaacacca atcgaaatat
    14821 ttttgagcta tgtacaagaa gcattttatt ctagcttaat ttgacaaatc ataacctata
    14881 aaaaaacaaa ataaaaaagc agaaataact ggctatttct agcggattat ttctgcaata
    14941 actaattatc tattttttat tttcaattaa tttaaagctt gaccatggtt tcaaaaagtc
    15001 aaggaggaat aattcgtcat cggagattct tcctacaaca ttgacacgtc cgtcgttttt
    15061 catatccttc aaggcgattt gtgtttctcc tttgtattga ccgtaccctt cattgtcaat
    15121 cagtacatcc ccatgatgca tatctcgcgt attatgaggc gggaaatctt tatctttgta
    15181 gtatacacga gtcatcgttg aacgaaggat atattctgag cgatcccctc gataactatg
    15241 aaggttatca aacaagcaga ttcgttcgtt ttctgtaata ccttcttccg tatctacttt
    15301 caatgttgga taatccgcgt taaatgcttc tgccatttct ttcaattctt cttcacttgc
    15361 gtacgcattt ccaatagaaa tatcatcaat caatcctgta aggattaaat gcttcacttg
    15421 tgtcgcaatt tcaagatctc ggtggtcttc cagcgtgcac aagccatctt gcgtaggcca
    15481 agggccaaac tcagcagatt gcgaattgac gaatgccatc gtattcaaat tatattttct
    15541 gaatttttct gaacaaaaaa caaagtgttc gtatcctaaa ccagagtatc gatgaggata
    15601 aaaattatga gaacctaata aattatccgt gtttggcgaa taactcatga tgttgtcaac
    15661 gtaattcgtg cccgaactca tattgatctc gattttgata ccaaaaggat tgcgcgtcat
    15721 ttttgcttct tctgctcctg tgaatccgat atctaagcgg accccgtagg cacccatctt
    15781 atggaaaaaa gataagtcat cgtaagaaat ctccaactgt tcaaataacg ctggattgat
    15841 atcaaccatt acttccatgc ctaggctatt cgcgtaatca actacttctt taaattctga
    15901 aagaactttt tctttatcat cattgatctg cagcaaactg gtaaaaaccc gtttataccc
    15961 atacttatga gctaaatcca agtacgcttt gtccttttca aatgttgacc gttctggata
    16021 aatcgagatt cctaattttc ccatattacc atttcctcct gcatcttttc gtttttgtat
    16081 ccacttacat tatagcaaac acaaagtaaa tggtctacac cttttctttt tcttttaaaa
    16141 taaatgataa attccaataa tttaagtagg tattttccct ctattttgtt atcattaaag
    16201 ataacaaaat agtaaattgg aaaggaacag ttatgaagaa aaataaagtt attttaagta
    16261 ttagtttagc agcagcattg ctgtttgctg ctggttgttc tgcaaacaac acgacttctt
    16321 ctagttcgtc tacagaaagt tcaaaaagta catctactgc ggaaagcacg actgccagta
    16381 gcgtcgattt gaacgcattg gaattaccac aattgacgaa agatgtcgcc gaaaatgaag
    16441 atctggtcga aatggtgacg aataaaggca caatcaaaat caaattattt cccgaacacg
    16501 caccaaaagc ggttgaaaac tttatgacac atgcaaaaga cggttactac aacggactga
    16561 ctttccatcg cgtcatcaaa gatttcatga tccaaggtgg cgatcctaaa ggcgatggca
    16621 ctggagggga aagcatttgg ggagaaggat ttgaaacaga gatcaacaac cacctttata
    16681 atataagagg tgcgttatct atggctcgct cacaagatcc taacagtaac ggcagccagt
    16741 tctttatcgt tcaaaataca gatgatatgc atgatggtct tttaaaagac gactatcctc
    16801 aagcaatcat cgacgcttat aaaaacggcg gttatccttc actagatggc cattatactg
    16861 tcttcggcca agtgattgaa ggaatggatg tcgtggatgc tattgcaaat atcgaaacag
    16921 atagttcaga caaaccaaaa gaagaagtaa aaatcgataa aatcaatata ctgcaagaag
    16981 caaaacaaaa ataaaaaaat aacgtctgaa tcctgcatag atgacaacgt atgctaggat
    17041 tcaggcgtct tctttttaca taaaaagcaa gtaagcttcc ttttgttttg ctatatcagg
    17101 ttttatagtc aagcattttg gttgacgatg aacgtgaact cgcactcaca gactttttta
    17161 tcatctacat atgctgcaga taacgctgta ccgactgatc ccttcatttt ggtgatttca
    17221 aaatgacatt tcagcacatc tcctggaaca actttttgac ggaatttggc cttattgatc
    17281 ccaccgatat aagctctttt tcctagaaac tcttctgatt ttaggataag tatcgaacct
    17341 gcttgtgcca gtgtttctag aatcaacgca cccggcatca ctggattgtc aggaaagtga
    17401 ccttggaaaa aatcttcatt gattgttaca ttttttgtcg caacgatctt ttttcctggt
    17461 tctaaagtat ctacataatc aatataacaa ataggataac gattaggaat caattccatt
    17521 acttcagtag cagataatac gtaagacata attaaactcc ttctttcagg caattgccat
    17581 tttcattact ttgactatca aagtatcata ttcctaaata tttagcaatc tt
//
LOCUS       DAYYME010000055        17261 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000055 DAYYME010000000
VERSION     DAYYME010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.055"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..537)
                     /locus_tag="ACHBFW_001498"
     CDS             complement(<1..537)
                     /locus_tag="ACHBFW_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM9317807.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAE"
     gene            complement(549..1241)
                     /locus_tag="ACHBFW_001499"
     CDS             complement(549..1241)
                     /locus_tag="ACHBFW_001499"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFM9317808.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1260..1544)
                     /gene="macP"
                     /locus_tag="ACHBFW_001500"
     CDS             complement(1260..1544)
                     /gene="macP"
                     /locus_tag="ACHBFW_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFM9317809.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1557..2120)
                     /locus_tag="ACHBFW_001501"
     CDS             complement(1557..2120)
                     /locus_tag="ACHBFW_001501"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM9317810.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2348..3010
                     /locus_tag="ACHBFW_001502"
     CDS             2348..3010
                     /locus_tag="ACHBFW_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFM9317811.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3134..4330
                     /locus_tag="ACHBFW_001503"
     CDS             3134..4330
                     /locus_tag="ACHBFW_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFM9317812.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4431..5807)
                     /locus_tag="ACHBFW_001504"
     CDS             complement(4431..5807)
                     /locus_tag="ACHBFW_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9317813.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5844..6711)
                     /locus_tag="ACHBFW_001505"
                     /pseudo
     CDS             complement(5844..6711)
                     /locus_tag="ACHBFW_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
     gene            complement(6800..7825)
                     /locus_tag="ACHBFW_001506"
     CDS             complement(6800..7825)
                     /locus_tag="ACHBFW_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9317814.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7843..8517)
                     /locus_tag="ACHBFW_001507"
     CDS             complement(7843..8517)
                     /locus_tag="ACHBFW_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9317815.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8701..9594)
                     /locus_tag="ACHBFW_001508"
     CDS             complement(8701..9594)
                     /locus_tag="ACHBFW_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9317816.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9668..11365)
                     /locus_tag="ACHBFW_001509"
     CDS             complement(9668..11365)
                     /locus_tag="ACHBFW_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM9317817.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11358..12671)
                     /locus_tag="ACHBFW_001510"
     CDS             complement(11358..12671)
                     /locus_tag="ACHBFW_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM9317818.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12671..16612)
                     /locus_tag="ACHBFW_001511"
     CDS             complement(12671..16612)
                     /locus_tag="ACHBFW_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9317819.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16721..17179)
                     /locus_tag="ACHBFW_001512"
     CDS             complement(16721..17179)
                     /locus_tag="ACHBFW_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9317820.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4836 a         3889 c         3009 g         5527 t
ORIGIN      
        1 ttctgcgcct agtgtggact caaaatgtct caatgcccat tcatgtccaa cgggatcgaa
       61 acttgccacg gaatctttga aaatatggag tttgtatcct agattatacg catcgactgc
      121 tgtatgcaac acacagatat ctgtacatac gccggtcaaa taaatatctg tgatctggcg
      181 ttctcgcaaa cggatgtcta gatctgttcc actaaaagcg gaataatgcc gtttgtctat
      241 ccaataaaca ttttcctctt gctcatagtc ttggtataaa ctttttaaag agccgtacaa
      301 ttcccgtcca cccgttccta tcacattatg cggaggaaat aagcggttct ccggatgaaa
      361 ggaatctttt ggatcatgcg catcaatggc aaaaacgaca aaatcctttt gatcaataaa
      421 cttctttgta tgagcagtca gctttttttc aattacttgt cctgctgtcc cagtagtcag
      481 ctttccatcc tctgcaacaa aatcatatgt gtagtcaatc gagatcaatg cgttcatctt
      541 agtcctcctt atacgagatg tttgacaaat tcgatcacca tttctgctga tcgtttgcct
      601 gcgtcttcga taaattcgtc aaagctttgt gtcgccgagt gatctgctgt gtcgctcatt
      661 gcacgtacga tcaaaaatgg gatattgaac tgacgagctg tttgtccaac agcggctcct
      721 tccatttcgc aagctaatgc ttcagggaaa ttcgttaaga tttctttgat tttatctggc
      781 gagtcgacaa atgtatcacc tgtaacgatc aatccttctt ttgcgttcaa attcgttgct
      841 tttgctgctt taaccatttc actgcgcaaa tatgtgcttg cttcataata tagaggcatt
      901 cctggtaatt gtcctgggtt gtagccaaat cctgttgcat ctgcatcaaa ataagctact
      961 ttgtctgata taacgatgtc acctacttgt aagccttcac cgattccgcc agctgaacct
     1021 gtattgatca ccatgttcac gccgtattgt tggatcaaca gacttgtggt aatagatgct
     1081 aagacttttc ctataccgga acgtacgacg atcacttcgt ggttacctaa tgaacctgaa
     1141 ataaataaag cgcctgcgcg ttcccatgat aatggttcgc ttaggttctc tctcaaaatt
     1201 tttacttctt cttccattgc gccgataata ccgattttca tctttttaat tccttctttc
     1261 tatataaatg cgattgctaa aaaaaccaca cataacatta ggataacaat aataagagat
     1321 ttcatcaaaa aagagttcca tttggtcgtc ttttcccgtt cgctgatcct tgttttcgtg
     1381 attggtttat tctttttgct ttctttacga taaaagctgg caatcttttt ttcttcttgc
     1441 tgataagctg tttctgcttt tcgctgtttt tttaatgact cgcttgcttg tgcttgttgt
     1501 cgcttgcgaa gttcgcttcg cgtaataagc ggtcctttgg ccataagccg atccctctac
     1561 tttcctttgg tcaatagttc ccagtattga atcgcataaa tcgtctttgc atcacaaatc
     1621 aactgatctt tcatcgcttg ttgcgcctct tcaagagtta gatgatacaa ctccagtact
     1681 tcgtcttcat cttgtgctaa aggattctcg accttctcta ccccttgtgc atgataaata
     1741 tgaaggactt catttgcaaa acctggtgaa agatacatcg aagtaagatg gctcagcgac
     1801 ttcgcacgat agccagtttc ttcttctaat tctctagctg ctgtcatttc gggattttgt
     1861 ccttctcctg gatcgatttt tccagctgga atctccaaaa tcactttttc taaaggtttt
     1921 cgaaattgtt tgacaagaag cagacgatct tgttcgtcaa aagcaatgat cccgacaccg
     1981 ccaggatgaa ataccagttc tctcttcgaa gttcctccat ctggcaaacg aacttcgtct
     2041 acagcaacat caataatttt tccgctatag atttctttgc gactgattgt tttttcttca
     2101 aattgctcat tgttcagcat atcttctcct gctttctttg taatcataac tgcttttttt
     2161 tcatttttca atttttctca aaaactcctt attagtttac catagataaa ccttagattt
     2221 tctctaaata tagaaaatcc ttctttagtc cgatactctt tctagtagtc cgtatgctaa
     2281 aataagtatg taatcgaata acgaaaagaa ttcaatactc aacgataaat gacatggagg
     2341 gtttattatg ataaaacgaa attggccgtg gcttctagca ttggtcttgc tatttctctt
     2401 tgtccgaaac aatagttttt cttttgttct aggactttta ttgataggtg tagttttagt
     2461 tttgtgggga ctctttggtt cgcgcaggaa aaagggaaaa caagaagaaa ttcctgcact
     2521 gactgacgaa cttgaaactc actatgaaaa aagcggtatg acagcgaatg aaatttcatt
     2581 ttttcgacaa acaatgaatc aaacaaaaaa tgagatcaat caattgcaaa tgaatatgca
     2641 gcagacagcc aaacttaaag caatcgatct ccgccatgat ccagtcaaag cagcgaaagc
     2701 tttattcaaa gaattggtga aagaaccaaa ccgactgcat gaagcaagtc agtttcttta
     2761 tacgcatctg ccgaatctag ttgatttgac aaataagtat aatgagatca atgaccatga
     2821 gatcaaaaac aaacagacct atgaaaaact agaagaaagc gcgcagatca tcgaccagct
     2881 gtcagcttta atcgcacaag attatcaaaa attcgttgct gaggatctag atgatatcga
     2941 cgtagagata tctgttgcga aacagagctt aaagcgtgat aataaatatg aaccagaaca
     3001 aaaggattaa ctcttttgtt cataaaagaa gactcggaac attcagatga tgtctggctg
     3061 tttcgagtac tttttctaaa gtagagacat tcatgtaaga atcgagaaca gattcaacaa
     3121 ggaggaattt tttatggatc ataaaccgac tgaaacaaaa gatgttacac cagttaacga
     3181 tacacttgaa gatttattga acaacccgtt ttcaactcca acagatacgc tgaccacttc
     3241 acaacaagcg gagattgatc agctccaaga gcaacagaca gctgctcgtt taattgacaa
     3301 actaccggcg gaacgccaag aacaagctaa acagctagca gcaaaaatcg atgctaatga
     3361 tgcacagtct gtcatcagct acggatcggc tgcccaagca aaattaagtg aattctcaca
     3421 atcgatgttg aatcatgtgc aagcacaaga tattggtcct gtaggagatt ccttaactga
     3481 actgatgtat cgtctccaag aagccaatcc tgacgaactg cgtgccggag aagggaacat
     3541 cttccagcgt gtcttcggga aggtcaaaca atcgatctat gaagtgacag caaaatatca
     3601 aaagattggt gcacaaattg acaagatttc agtgaaatta gataaagaaa aagacggtct
     3661 gctaaaagat aatctgatgt tagaacagct ttaccagaaa aacaaagact actttgatgc
     3721 actgaatatt tatatcgctg ctggagaatt gaaaatggaa gaacttcaaa caacgatcat
     3781 tccagaagcg atgaaaagag cagaagaaac tggcgatcag atggatgttc agattgccaa
     3841 tgattacacg caattcctag atcgtctgga taaacgtacg catgatcttc gcttagctag
     3901 acagatcacg atccagcaag caccacaaat ccgtttgatc caaaacacaa atcaggcctt
     3961 ggctgaaaag atccaagcat ctatcgctac agccattccg ctgtggaaaa accaagtcgt
     4021 gatcgcattg accttgcttc gtcaaaaaga tgcagtaact gctcaacgcc aagtctctga
     4081 aacaacaaac gacttattga agaaaaattc agaaatgctg aaaatctcag ccatcgaaac
     4141 agcgaaagaa aatgaacgtg gaatcgtgga tatcgagacc cttcaaacca ctcaaaatga
     4201 tttgatcgaa acaatccaag agacattgcg tatccaaaaa gaaggaaaag aaaaacgtcg
     4261 tcatgcagaa gtagagcttg gacacatgga agaagatctg aaacaaaaac tgttggattt
     4321 aacccaatag atcacctttc gcttgttact aaaacaaacg cccaacaaca gtttattaca
     4381 actgttgatg ggcgtttttc ttatctttct tctaacttct ccattggagt ctatttctca
     4441 ttcatcttgc tgtgtccata gccatagcca aaataaatga tcagtccaat aaccattacg
     4501 ataccgaaca cgatccatgt cacagtttgc agacgaagca tcaatacaat acaagtcaaa
     4561 atggagacga ttggcaatag cggaacgaat ggcactttga attctccagc ttttggtttg
     4621 ccaaacattt ttctcaactt cagtaagcct agactaacaa gcatcaaata ggcgagcgcc
     4681 atgatattcg ttagttccgc taatacattc aatgggaaaa atccggcaaa gaatacagct
     4741 gccacaccag cggcataagt cgcttttttc ggtgtacgat gcttttgatc gatttctgtc
     4801 ataaacttag gaagtaagcc atccttgctg acagcaaaca gtaaacgagc aagtgaatac
     4861 atcatcgaga tcgttaccgt cagtagcgtt aaaattgccc ctacagcgat caccgcgccc
     4921 actgttccat gtcctacata cctcattgca aaagccactg ggtcattgac tcctagttca
     4981 gtatacggaa cgattcctgt caagatcagc gtcacgatga tgtaaaggac agtagcaatc
     5041 agtatcgaac caatgattcc tcttggcaca tctttttgcg ggtttttgac ttcctcggct
     5101 gccattgcaa cggcatcaaa tcccaagaaa gcaaaaaaca caagagctgc accatctagc
     5161 acaccggaaa aaccaaaagg catgaatggc tgccagttat caggtttaac gaaaaatatt
     5221 cctacaatga taaacaacgc gatgatacca aatttgacat ataccatggc attattcaaa
     5281 cgtaatgctt tcttggcttc aagcgacacg atgtaagtga cgaatatcac tacgagcacc
     5341 gcaatcaagt caatgtatgt accattttcc ggattgtacc cgccagttaa tgcttgtgga
     5401 agttcggccc ctaaactttt caaaaaacct tgcatatagc cagaccatcc ggaagcaaca
     5461 gaggaaaccg ctagtaaaaa ttccccgatc aacaaccacc ctgtcagcca ggcagcaaat
     5521 tcaccaaata caacatacag ataggcatat ggcccaccaa tcactggcac tcgcgaagca
     5581 aattctgcga aacttagtcc agacaaaatg acaacgattg cagctaagac aaatgaaagt
     5641 gacagtgctg gaccagcatt ttgattggcc gcaacccctg tcaccacaaa tattcccgta
     5701 ccgatgatcg caccaattcc aagcatgatc aaatcagacg tttttagttc ttttttcatc
     5761 tcactcgtat gattgagatt gatctctttc ttacgaaaca aatccattga agttcctccc
     5821 cattttaggt tggaaaacag cgattaaact gttttcatat accctaaaag ggataccacc
     5881 taaaatgact aaaaggcaac caacaataac ataaatgagc tcttttttcg tttttctttc
     5941 gccaaggatg aaaatcccac ctaaagtaga gataatgatc cccatttgtg aaagtgagaa
     6001 gccaacagct aaaccaagtg atttgactgt tagaagcatg cagatgtttc ctaagcccca
     6061 taataatcca cagatcatgt ttttccacac aaactgatcg actttaactt tacggaaagc
     6121 aaagaagctt gcgcctaaga tcattccgat actttgaggt aagataattg acatcgcatc
     6181 caaattcgcc cagttgacga tgatggtata tacaccataa ccaatcgtag agaagatcaa
     6241 tgctctaaaa cccttaccaa aatcaagcat tttattatct gtatctattt ttccttcgtc
     6301 atcttgtcgg gcagtcaaat acgcaccgct tacaagaaga gctaaagcaa taataccgta
     6361 tacataatct ttcgtttgtg tccattcatg gaagaaaacg gctcccgcaa tcgtattcaa
     6421 aattaattga aaaccagtag acaatggtag ccctacagaa acacccatgt acttcattcc
     6481 atggaactgt ccattttgtc caacactcca agctaagccg gataaaaatc cgatcaagaa
     6541 aatccaaggg gtgattacag gactaacaac gaaaaagaca actaaagaaa acagaaatgc
     6601 accgatcgtc attcctagtg tttgttgatt ggcatcgcca ccaattttac cacttactaa
     6661 accaatacta ccccatgcca acatagggac taatgctatt aaaatttcca taaccaatct
     6721 ccttttattt cacatgataa gaaacttatc agcgaaaacg ttaacaacac aagcactttt
     6781 tttcgtttct aacgattttc taacgttttt cattataaaa atttaagatt gggaaaaata
     6841 aagtcattgt tgtccctttt ccttcagttg attcagccgt caaatggata cctaattttt
     6901 cggccagatt ttttgctaaa taaagtccta atcctgttga atgtgttttg ctaagtcgac
     6961 catttctccc agtaaatccc ttatcaaaga tccgtttcaa gtcttccgct gggataccga
     7021 tccctgaatc tttaactgaa aggctgactc cttgcgcttc cttttctatg ctcacaatga
     7081 tgtttccgtg atccggcgta tatttgatgg cattactaag caattggttg aagataaaag
     7141 cgatccattt agcatcggtc aaaacagtat gatcctcacc aacgatctcg tagcgaatat
     7201 ttttttggat aaaataattt gcctgactgc gtatcacagg ctgaatgatt ccttttagtc
     7261 catattcttg gatgaggtag tcccgcgaga aactgtcgag tcttgcataa tacaaaactt
     7321 gttctacgta ttcatcgatt tttgataatt cattttctag taatgtatac ttttgatcgt
     7381 caatatcgaa ctcgatcgaa cgaactagca atttagaagc ggaaagtggc actttgattt
     7441 catggaccca tgagtcaata taatcttttt ggtcttgttg attactgatt gctgattgca
     7501 ttacttcttg gtgttcacgg atcagctgat tgatatattc ttctaccagt ttttcttctt
     7561 ctgtcctccc accactcaaa taattttgca aaggggaagt tttttgtatg tcggctaatt
     7621 ttccccacca tcttctcttg ctcaagtaac cacccagcag ataaaaaatc agaaagacgc
     7681 cctcaatcaa agctaaataa gcaattgtgc tcaaatcaac tttgatgttt ggcgccagcc
     7741 acatcacaaa gatcgtcaat gcaataaaaa acagccatcc caagatcaat gtccactgat
     7801 ctttcaaata tttaaaaaaa ttcatcttaa ccgctcatcc tttcagggaa cgatatatcc
     7861 ctgtcctact tttgtttcga tatatccttg gatacctgcc tgttcgattt ttcggcgtaa
     7921 ccgattgatg ttgactgtca atgtattgtc atctacaaat cgttcgtcat cccacagtgc
     7981 tcgcagcaat ttttcccgac ttaagatctt tccattgtgc ttcatcaaaa taaataatag
     8041 acgatattca tttttgctca aatcaatgat ctctccgttg atttccatac ttccgttgtc
     8101 aacattcaac gtaatgccat tgtggctcat catttcacta gataattctg tgtagttgta
     8161 agaacggcga agtaatgcat tgatttttgc aatcagtaca tcgatttgga aatgttttgt
     8221 cacaaaatca tctgccccca tgttcatagc catgatcata tccatattcg tattcctgct
     8281 ggagataaaa atgatcggta ctttcgatac ttcacgtatt ttttgacacc aataatagcc
     8341 atccaaaacg ggaagattaa tatctagtaa tacaagctgt ggttcttctc tttcaaagtc
     8401 tgagaacact tgattgaaat cggtcgttcc aaaggtttca aattgccatt tctcaagttc
     8461 ctccatgatc aattgtcgaa tgatctcttc gtcttcgact accatgattt ttgccataaa
     8521 aaatccctcc atctttcttc ttgctactac aatatcaaag aagcccttgt gaaacaagtt
     8581 ggcttacctg gaaaatgtca ttttagcaaa cgttaaaaaa aactggaaat atgcgattta
     8641 atcgcgtttg tctcttccaa gcagcttaaa tttattatgt tattcttaat aattggtcta
     8701 ttatctgtta tttttctttc gttgtttttg tcttttaaat tttcgataac accaataacc
     8761 gaaaagcgtt agcactagca atatacctag aaggagtata agtaatcgat aaagatggta
     8821 tttttttgtc tgttctatct ttgaagagac ttcttctgta aagggaactc gtcttcctgt
     8881 cactagtaat cggtgagtgt tcacggtgta aggtgtacaa gtgataagtg tgactagatc
     8941 ctgcttttct ctaattgcta atgtatttgt ttttgttgga aggacaacaa ttttctcaac
     9001 gacttcataa gcgagcatcc gaccatacac ttcaagatag aatcggtcat cttgctccat
     9061 ttcttcaaga tccgtaaaga gttttttctc agcaataccg ctatgggcgg taatcacgga
     9121 atgtgtactc tctcctccag ttggaaaaga ggtgccttgc aggactgtcg ctcctctatc
     9181 taacaaaaga tcattcgttt catcaaataa aggcaagcta acattgattt tcggaatata
     9241 gatggctccg atcatatgtt gttcgtaata ttcttttcct ggattttgga catttctaac
     9301 cgcttgctca aaaggatctt tgacttgtcc cattcctggg attactgatg tatgagcaag
     9361 cgtctgattt ttcttttcat gcactgctaa ttgttgctca tgtttttctt ctttatcgcg
     9421 ttcaagctgc ttttgataat tctcgatcct tctttgatcc aaataattgc tgagagcatc
     9481 tgctaaaaat ggatatgtaa aaactgcagc tccggcaaga aacatcactg ccataaataa
     9541 ttgtaataaa agactgattt gggtttgagg tctattacct attttctttt tcattttctt
     9601 caccttctta tttaaaaaaa ctgcagcaac ctttataaaa ccgattgctg cagcttattt
     9661 ttgttgctta tacttcagac ttcattttag atttacggta ccagctgtac gctccaatca
     9721 tcaaacttat tccgattagt aagaatgcca agagcccgtt cccaccagta gcaggaagtg
     9781 acccgcgttt catattcggt actctttctg gccctggatt ttctactcct acgatctgaa
     9841 tttcttcatt atagcttcca taagagatga taaattcagt aaaggctgcc tcacctgttg
     9901 gaagtacata accttctggc gccatggtct ctttcatttg ataggttcca taatctaacc
     9961 cttgaacctc aaagtatcca tttccatctg cgacaaattc tgttgcttgt ccgcttgttg
    10021 cccactcaat tctatctgct cctgattcat aaacagctaa aggcgactcc cctttataaa
    10081 atattgcgta ttcagaacgg tcttcattcc acaattcgaa tcttgcatct ttaagcaatt
    10141 cactgcttga tgcatcatat ttttcaaact ttcttccacc tgttgtgact ttctctggcg
    10201 gctctactcc ttcttcataa tcaaattcat catttcgtcc gatttcaata accgctgtgt
    10261 tgttatggaa atcatccgct tctagttctg tggaaacctt caaattatac gtgatcgtta
    10321 aatctcctcc acgtgcatct gctaatctac ctaaagtatc tactttggct actccatttt
    10381 cttctgtcaa aatcgtcacg acaaacccgt tgcttactac ttctactata taatcttgtc
    10441 cttttgttaa aatcgtctcg ccggctcgca cttctatttc ctgatcataa taagttagac
    10501 caactgtagg cgtatctgtg attttgtaat ttttgataac agcgcttccg ttttcaagga
    10561 cagaatcgat gaaataagga ataggtgctg tgatcgtgta agaagccgtt tttcctcttt
    10621 ctagatcaat ataagaaatc gtttcttgtt ctccagcttc attaataatc gttacttgtc
    10681 taaggtcgct ttcctctaca ttcatctgtt tatctgcttc tctgatttcg tttttcggat
    10741 acaaatgcac atctgtattg tatgtgtctt tccatgtaga accatcccac acgccatcca
    10801 ccatatcagg catcatcact ggcatcataa caaccattgg agacgcgatt ctggaaatat
    10861 gagctggcga ataagtttct aagaaaagat agactttatc tctagaagaa ggtgcttctt
    10921 ccgtcacttg gacggtagag aataccgctt ctccagcttc attcgttgtt tgtttgtcaa
    10981 tacttgtcag tcgatatttt aaaaaccaac tttctgtgtg ccgtttttga atccattcaa
    11041 tcgcttcggc agaattaaga ccgtcttctc gttccggtgt taaaggatta tctcgaatca
    11101 aatcgtaata tacttccgtt acgtcaaaca aatcaaaatc gactcctgga atattttctt
    11161 caccaaaatc cgtagaaacc aatccgctat tcggtcgttc ttccggaaca gatgagaaac
    11221 cttttttgtg taatgtaatc gtcacttcac ttggtttttg tgcgacttct tcggctttga
    11281 ctagttttcc gttcccgaaa atccctattc ccgcagtaac gagtaacagt acactcattc
    11341 ccgtgacaat cattttttta ttcatttttt tctccccgtt ctttttttat taataaaaca
    11401 gcacctaaca ataaaagaat tcctagtaaa gaaatcaacg ttcttgcttc attcgtttgc
    11461 gggaatacgg accattttcc tgattgacct ggattgcttg gaccactcgg ctctttcggt
    11521 ttgtccgttg tttcttttac ggtctcgtta taaacacgtg gtgttgcgga ttcataggca
    11581 gagactggaa ctttcccgga ttctaattca gccatctcct gtccgttaac tactacatat
    11641 tgtggttggc cttttgcatc cacccaaaat tcagggatga tgacctcgat tctcttactt
    11701 gcttcatcca tctcgtaacc ttccacacct tttaattctt caaaataata ggtgccagaa
    11761 ggcaagaaac gttgtcctgt cgtaacaagg ccctctttat ccgaaatgaa tttgcttaca
    11821 tttttgtcat tcaaaggatc attgtctgct ggtttgatcc attgattttt caagtcatta
    11881 gcaggagcca tatctaaata atatttcccc ccttctatca tctgatatag agcaaaaaca
    11941 gcaccttcta atggttttcc tttttcagtt gttcctcttt gacgtccata cttgaagaag
    12001 tatggatctc ttagataacc gacattttta ggatagatat gaatctcttg cagttcctcg
    12061 gttgattctg tcgggtgata aattggcaaa atagcagcaa ttgggatagc tgtctgatcg
    12121 atatcaacat ccaatccaac ttctggatca atcgattctt cgaaaatcaa ataaactgca
    12181 tcccgatctt ggtttttctt aggtgctttg atacgtgcaa tcccgtctcc taatccgcca
    12241 accgtatctt tcccagttgt taccgaagtc cctatttgga caagcctttc ttctgtgatt
    12301 agctgacgga tattgtttct gttcgtttca gcgatttgct ggacgaattc tttaggagtc
    12361 atgttttcag cagctttttt ttcatgataa taatcggtca tgtcgtaaat ggcaaatgtc
    12421 gctccattta gtggcaccgt ctgtgaaatg atatctgctg tttcccctgc ttcctcagag
    12481 atagccaagc catcattttc atggtattca aattgatcgt tataatcgat atctcgaatc
    12541 atccgtttat gcaacacgaa ttccacgaca tcttcccctg attcttctgc cgacatcgca
    12601 tgtggaagaa ggactgctgc aagcggtagt aaaacaagta attgaagcat caaacacaac
    12661 agctttttca ttaccttgct ccctcctttt tagattgtct gacaaacaaa ccataggctg
    12721 ctcctgcaag cgccatgaag aatgcagcaa gcaggatata gaataatcgt ccggatcctc
    12781 ctgtagcagg caacggattt tcaggatcgt tcccgacact aagctgtaat ctgattcgat
    12841 ttgtctccaa aagttcatat tctaactctt gctgtagatc aggcgtctct gcttcttcgt
    12901 ttggataata cgaaatctca acttttagtg tacccgcttc ttttcctgga actacccgat
    12961 cattttctat gtcaggttct tctcgataac taatttctaa aataaagtaa cctggaagtt
    13021 ttctatagct gtcaggtgac ttcacttcct tgatccgata gaccccttcc aacaagtctg
    13081 aagcattgaa tagggaagaa tcgtttggat ctggtgccaa gcctccctcg atgatttcat
    13141 actctcctgc actattttgt ttctcaattg aaaattctgc gccttcaaga cgtcggctat
    13201 tcatactgtc aattttcaaa agttccaaat cgattggcgc tagttcatct tctaaggtta
    13261 cttgtagtac accatcgatg atttcatagc cttccggcaa gggagcagtt tcgtcttcga
    13321 tgaccgtatc ttcaatgata aacttacctt cttcattcag tgcaaactgg aacacttgat
    13381 cagatagccg atacccattt ggtgcgacag attccgagac ctcatattca ccaatagaca
    13441 gatgcagagg ttggtcgtaa tttaaatcga catacttttc tactcctacg gcacgagaat
    13501 agaagtatcc gatcccaggc aaacgtttcg attcttctgg ttctaacgga ttaccagtgt
    13561 atgctcgata cagcgggaag gaagaatccc ccactttcct gatatcgaaa cctgcaccat
    13621 ctaatgtatg gccttcatgg tctaattttc taactcggat ctcccgataa tcaaacgtat
    13681 tgtaagcagt gaatgctacc cataaacgat cctcttcatc tgttgtatac gtgagatatt
    13741 gccaattttc ttcacctgtt tgcttgagtt ggatagtccc tatagcatta tcgtaaagtc
    13801 gcaagatcca tgtgtcattt tcatcaggtt ctttgaaacc ttctgggttc tggctctcaa
    13861 tgattttgta ttctgcatag ttggtagttt cttcactatc cttataaaca aatgaatctg
    13921 gctctccgat tggatgaata actttctcac catccacgat tacatatttc ccgtcttcat
    13981 ctactgaata gaacgtgagt ctccctttct cttcctctac ttcgttcaca ccagaaagcc
    14041 ctttcgctac tggatatccg atttccagtc cgttaccatc tgtttcataa agttcaaact
    14101 ctgcgccatt gattggcata ttactgcctt caattagttt attgatattc atgtggattg
    14161 gcttgaattc atttcgcatt tccagtttga catacaactc atcatactct gtgccttcta
    14221 tgttaggctc ttgtacaaca gaaagcttgt gatattctcc ttcataatag gaattcggat
    14281 ctttaccttc cggtccgccg actctcgaac cagatgtata ttcatctaca agctcgtagt
    14341 gacctaacat ctcatcttcg ttggctgcat attttttgat ttggaattga tacgtctcat
    14401 ctactaactg ataacgtggt ggtccagcga tctctctgat ccaaaatatg tcgctgactg
    14461 ctccagtttc atctcctaaa atcggcatct tcgtttcaaa gatcgctatt ccgtccgtgg
    14521 tagttttact tgtttcgatt ggttcggctt ccccactctc tggattttgg tcatataatg
    14581 caaattcgac tccagccaac ggctcgccat tttgaccctt cttgctgact tggaaattac
    14641 ggcctttatg ttcgtaacga atggaagaag atgggaaata catccgtcca gtagtgatat
    14701 cattctcata atcctccagg ttctgaatat aggccatatt gttcactgtg caaaaatttc
    14761 cgtcttgagc tcccgcatca agttttactt ggtacttgat cctgaccatt tccccttcac
    14821 ctaaagaaat cggagaaaat ttataagctt ggttttcccg tccgacatac gttgtcggag
    14881 tcgccactac ttctggtggg gcttgtccat gacgttcttc atcccatatt tctggctctt
    14941 ggtacttcat gccgttttct cctggatcta gatggtaaaa ctgaatatct gccgatctgc
    15001 cagctccaaa tggtgagaca ttaaaaccag tttcatcata aagtgtggct tgctggatcg
    15061 ttctcttgaa ggtagaagag atatcctcca aggctgtaac aatgtcattc ttgttgttcg
    15121 cattgtaata tcgcggtgtt acgccatctt cttctaaatc agcaatattt ttcaatacat
    15181 ttcgtccaat agcatctagt cgttgtctcc cgctaacgtt tccatcgatt cctaaaccaa
    15241 tggagaagat acttggcggg ttctcttgat acttgttttt cagccaatga tgataagcaa
    15301 ctgtgttgat cgcagatgac ttttcttgcg aagtaggact gccattcgta ttgttattag
    15361 aagaaccatc accaacccag tgagtcgctt tattatctcc ttcttttacc tctccataac
    15421 gatagtgtac atttacttca tcttctggac ggttaaagtc agatgcatag gtaactggat
    15481 gagtaaaccc tgcattagaa gtattcccag aaaactcctc aaaatttctg aacaaaacgt
    15541 cattatcttc agcaatctta tcttccatca cagtccagtt gctccaagct ccattagtgc
    15601 tcgtacgata acttgactga atcggtgcat aactgaaggt cggtgtccca tctcccacaa
    15661 cgattaggat tttttctgga tttctatttt ctccgccatt gtctttatac aatgtttcat
    15721 agccattttt aagccctagt gtaagcggag ttcctccgat tggtgtacga tttaatgctg
    15781 tattctcact tcccgtcaaa cgattgatat catttgtcaa tggaaagatg tccgaactga
    15841 tttgctcatg gttattgata gctgtactat tatggtaaaa attcaccata ccgattcgaa
    15901 tatccagact cgggtcagag agcagatctt tactcatttc gtttactgcc tcaatcaacg
    15961 cagcattttt agtttgacta ttggtcccag cggttaattc actcatactc gctgacttat
    16021 ctagaacaaa aacgatatcg attggactga tttccttttt ctcgcccccg atgacatcta
    16081 gatagacatt gtaaagggtt gttgcatctt caggatgtag atcagatgct gtgctgcttg
    16141 gcagaacagg ttctacccat tttttgatgt acgccccgtt ataatccaag taactgtttt
    16201 caaaattccg ttcatttccc caaagctgaa cggtttgagc tggaacgttt ctatcttctt
    16261 cgcctgcagt tgatccatta ttagtagcat agtcgtaatt tcgccagttc gctcccccgt
    16321 ttgtataatc actatcatcg atcaagttct ctccgtcata tggcgagcct ttgtctcctt
    16381 gatagcgttc attgctttgt gtccaatttc ctaacacttt tgtatagtca ttcgttgtaa
    16441 acttcgggta gcgatgctgt tcgttttcaa cttctccgct aggatagata tattcatagg
    16501 ggtcttgcgt agctgttggc ccaagacgct ccaaaatacc gttgaataac tggcgttgaa
    16561 tgcgctggag tgcttgaccg ctgggtacga ttggttcgtc cagcttttcc atctcatttt
    16621 cttcgctacg ttgaatttca gcggaatctt ctgtatcagt ttcattttct tgtaattctt
    16681 ggttatctat tctttcatca accaccactt gaagaggtag ttacccttcg ttcacatcgg
    16741 ggatcttttc tgtttcaaaa atcaatgtgc cggtttctgc ttcttttgaa taaacttttc
    16801 cgacatacca ttcttgacca tcgatcgtct cgacttttag gtcttctcca tcttcatcat
    16861 aggaggatag acctgttcca cgaacgtttt ttacggtacc aatcccggcg gctgccgcat
    16921 cgattgctaa acgtattcta ccttcattgg cactttcctt tttttcaaaa gccagatgcc
    16981 attcaagggt ttcttctttt tcaatatact tcccttcaat ttttgcatag tcctcatcta
    17041 agaatacctc atcctccttt gtttctgttg actcagcagc tacatccagg gtcgtcatat
    17101 gatagactga ctggaaatat ggtccaaata gcaaactgac agttactaac tgcaaaagct
    17161 tcaattttaa ccctttcaca tccttatact cctttcaaaa atatttgtct tctttcacaa
    17221 caatcaacaa aacaatgccc atctaattat aatttaattt a
//
LOCUS       DAYYME010000056        16869 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000056 DAYYME010000000
VERSION     DAYYME010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.056"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..1757
                     /locus_tag="ACHBFW_001513"
     CDS             57..1757
                     /locus_tag="ACHBFW_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFM9317821.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGRGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            1727..1945
                     /locus_tag="ACHBFW_001514"
     CDS             1727..1945
                     /locus_tag="ACHBFW_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFM9317822.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            1965..2855
                     /locus_tag="ACHBFW_001515"
     CDS             1965..2855
                     /locus_tag="ACHBFW_001515"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFM9317823.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            2873..3157
                     /locus_tag="ACHBFW_001516"
     CDS             2873..3157
                     /locus_tag="ACHBFW_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317824.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            3644..4258
                     /gene="gmk"
                     /locus_tag="ACHBFW_001517"
     CDS             3644..4258
                     /gene="gmk"
                     /locus_tag="ACHBFW_001517"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM9317825.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            4263..4568
                     /gene="rpoZ"
                     /locus_tag="ACHBFW_001518"
     CDS             4263..4568
                     /gene="rpoZ"
                     /locus_tag="ACHBFW_001518"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM9317826.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            4821..7229
                     /gene="priA"
                     /locus_tag="ACHBFW_001519"
     CDS             4821..7229
                     /gene="priA"
                     /locus_tag="ACHBFW_001519"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFM9317827.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            7244..7735
                     /gene="def"
                     /locus_tag="ACHBFW_001520"
     CDS             7244..7735
                     /gene="def"
                     /locus_tag="ACHBFW_001520"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM9317828.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            7728..8666
                     /gene="fmt"
                     /locus_tag="ACHBFW_001521"
     CDS             7728..8666
                     /gene="fmt"
                     /locus_tag="ACHBFW_001521"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFM9317829.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            8666..10024
                     /gene="rsmB"
                     /locus_tag="ACHBFW_001522"
     CDS             8666..10024
                     /gene="rsmB"
                     /locus_tag="ACHBFW_001522"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFM9317830.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            10037..10777
                     /locus_tag="ACHBFW_001523"
     CDS             10037..10777
                     /locus_tag="ACHBFW_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFM9317831.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            10774..12843
                     /gene="pknB"
                     /locus_tag="ACHBFW_001524"
     CDS             10774..12843
                     /gene="pknB"
                     /locus_tag="ACHBFW_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFM9317832.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            13008..13907
                     /gene="rsgA"
                     /locus_tag="ACHBFW_001525"
     CDS             13008..13907
                     /gene="rsgA"
                     /locus_tag="ACHBFW_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFM9317833.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            13920..14570
                     /gene="rpe"
                     /locus_tag="ACHBFW_001526"
     CDS             13920..14570
                     /gene="rpe"
                     /locus_tag="ACHBFW_001526"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM9317834.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            14571..15209
                     /locus_tag="ACHBFW_001527"
     CDS             14571..15209
                     /locus_tag="ACHBFW_001527"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFM9317835.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            15364..16218
                     /gene="mreC"
                     /locus_tag="ACHBFW_001528"
     CDS             15364..16218
                     /gene="mreC"
                     /locus_tag="ACHBFW_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFM9317836.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            16222..16731
                     /gene="mreD"
                     /locus_tag="ACHBFW_001529"
     CDS             16222..16731
                     /gene="mreD"
                     /locus_tag="ACHBFW_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFM9317837.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
BASE COUNT         5654 a         3039 c         3693 g         4483 t
ORIGIN      
        1 gagactcatg acctttttat gacgtactgg tatacttgaa gaaaaaggag agcgcgatgg
       61 agataaaaaa agtttacttt tcacagaaaa tgtcgctgta ttcgcctatc cagcaagcaa
      121 aacaacccct tccatttttt caaagtcatc aagaaagtag agacgctttt tggcgttctg
      181 caaaaaagca atgtgctttt aaagcagtat cgaaaaaata taccgtctat tttactattg
      241 ctaaaagtag aggagatgtt atttttaata atctgttgat tgaaggtgaa acttttgaat
      301 ggaaaaaatt ttttcggtat atggaagctt gcgctcgttt ttttattaaa gaaaactgtt
      361 gttttttatt tcaatctcct ctatcagaag aatggttgcg tatttttcat aaaaacggct
      421 atcaaggaac cacacaatta aataaaaagc tggtctatca tacagcactt gttttaggta
      481 gaggaggagc gcacggtgct tatcagattg gtgtgtggca ggcgctgaag gaacatgata
      541 tcaactttga gattatcaca ggtacctcgg taggtgcgtt gaacggcgca ttgattttac
      601 aaggagatat gaaaaaagct gtgaatttat ggaaaaagct gtcgacaaga caagtgcttg
      661 cgttaccaga aatggctgca gttgaagacc ttcgtgaacg cttctatcga gaaagacgcc
      721 aaatgacgaa aacagcacta attgaaggcg gggtttcttc tgctccattg gaaaagatgg
      781 tcaaagacaa tttggatttg tccttactca aacataatcc caaaatacgt ttatatacgg
      841 tgtctactaa actgccagaa ctagcggaag tagtgactga tatccaacag acaaaaacag
      901 aagaaatacc agattggata ctcgcctctg cttcatttta tcccgctatg gcttatcgaa
      961 agatcggaaa aaataaatat gcagacggtg ggtatcgcaa caaaatacct attgatattg
     1021 cgattaacaa aggagcaaca gaggcatttg tcgtagatgt gcaagggcca ggcccagcga
     1081 aaaaaattcg tatgccagac atttttatcc attggaaatg tcaaacattg tggacactag
     1141 gcagctttct gctttttgat agccaaagga accaattaaa tttacaatta ggctatctgg
     1201 aaatgaaaaa acgattaggc tattactatg ggaactggta tacatttcat tctgttaaac
     1261 ctgcaagaac atactggcga ggatttttgt cttacttaac aaaagaaatt cagttagaat
     1321 tatctttttt caaaagcata aaattttggc aaaaactgcg taatttatat aagaacaggg
     1381 tcgtaccaga gacatgcgga ttggcgatgc tagaactttt agcaaaaaaa cattttttac
     1441 tgcccaatga aatctatcaa gtagacatga tgattcaaat gatctgccaa caagatataa
     1501 aaagtatgcc ggatgattta ttggcacaaa ttggtcaatt aagtactgaa gaatggcgaa
     1561 ggtatcgaaa ataccagcaa aaaatacaaa atgagcgcac gaaaactgtt tattttgatt
     1621 atctgctaca aggaaaacgc aaggatcgtt tgcagcatga attgcttaag caaccagttg
     1681 acacattact gatcctatat ttatattatc tcaaggagga acaaccatgg cacaagaatt
     1741 ttcatatgaa atcgtagaag aaatcgctat attgtcagaa aacaacaagg ggtggcgtaa
     1801 agaactgaac ctagtcagct ggaatggtcg cccgccaaaa ttcgatcttc gagattgggc
     1861 gccagaccat gaaaaaatgg ggaaaggcct gacattgacg aacgaagagt ttgaacaact
     1921 gcaaaaagca atagaaaata tgtaatgagg aaggaccctg tcgaatgaaa agtatgactg
     1981 gttttggcaa agccatccgc gaaacaaaag cgtatcagtt agatattgaa ataaaaagtg
     2041 tcaaccagcg ttttctagat gttcaaatac gaagtcctaa aatcctgaac tttgttgaaa
     2101 acgattttcg tcagcttatt aaacagcagt taagccgcgg tcgaatcgaa gtatttgtga
     2161 atctagctta tattggcaaa aacgaaaaac agatcgtcat caactgggaa ttgattgatt
     2221 cacttatcac tcaattgaca gacggaattc aacaacgata cggtagtcaa aatcaagtgt
     2281 caattagtcg gatggcggaa cgcttcgtgc tggacgaaaa cttcgtaacg gtagaagaaa
     2341 aaccggaaga gagcatggaa gaattggcac agttagctct agatacattg aaaagcgcac
     2401 ttgattctat tgaggcgagt cgagaaaaag aaggtcaagc tttagcttta gtgttgaaaa
     2461 agaatactga agaattcaaa gaagtactaa aaaaactcga tagttttgta gaaatctatg
     2521 aaaaggaatt tcaaataagg tatcaaaaaa agctggagga atttcttgga gcgaaagtcg
     2581 atgagcaacg gttgttgacg gagctagcca tcttgttgga acgcggagat attcacgagg
     2641 aactggatcg tctggcgatc catgtacaaa aactagatga acttatcgaa gcacaatgtc
     2701 ctgtaggtcg ggaattagat ttcctgatcc aagaaatgaa tcgggaaatc aacacgatcg
     2761 gttccaaatc aaatgcaatc gaaattaaaa atcaagtcgt ccagtctaag acgatcttag
     2821 aaaaaatcag agaacaagta caaaatattg aatgacgaaa gaggtagaga taatgaaata
     2881 ttatgacgta acgttccacg aattaagtgg aaaaactgtg gttaaacggg atatcccttc
     2941 tgaaaaaaac ggctttgacg tatggaaaga tgcctgtgca gactataatg agaacgaact
     3001 gtttattttg atcaacgacg gtgcttacgt gacgatgaat cggaaattta ttgttcgaat
     3061 tgacacagaa gaggtagaag atccgacaga aaaagcacgt tctcgaaaag atgagatcat
     3121 gggtgtagtg aatactttgt ccaatatggg cttttagcaa ggatgctgaa caaagaaata
     3181 caataaataa acagaattgg atttcttcgc ttagcttctt tgctctagga atacatttaa
     3241 aggttgtgac aaaattcgtg tcacaatctt tttttatttt gaataagtga tgttcaaaaa
     3301 ccattttctc ggtattaagc caaatttatg caaaaatgtg agaaactatt ttcacaaaat
     3361 ttttcttcat actcgaaact aatcggcttc ttcacgacct cttatacacg gcgttcaaaa
     3421 gcttgcatct gcgataataa ccccaaccaa acgaaaaaca tgagaagctg tttatgtttg
     3481 gctgttctta ttacttgatg caggacagct tttttacgac ctcttggtac agggtagctt
     3541 ttttaccaat ttctagctat aattaaagat aaccttgaaa gttgcagaaa aaaagtgcaa
     3601 aataaacgat aagtacaaaa agactagttg aaaggacgtc gtcatgtcag agcgtggatt
     3661 attaatcgtg ttgtctggtc catccggtgt aggaaaaggc acagtacgaa aagcaatttt
     3721 tgagagtgag gataatgatt ttcagtattc gatctcaatg actacccgta aaatgcgtga
     3781 aggggaggtc gaaggcgttg attatttctt ccgaagcaaa gaagagtttg aatcaatgat
     3841 cgaagctgga gagatgctgg aatacgcaga atatgtcggg aattattatg gaactccttt
     3901 gtcatatgtc aacaagacat tggatgaagg aaaagatgtt ttcttggaga ttgaagtcca
     3961 aggagcaaaa caagtcaaag aaaaagtgcc cgatggtgta ttcatctttt tgactccgcc
     4021 agatttagcg gaattaaaat cacggatcgt aggccgagga acagatgcag atgaagtaat
     4081 cgaaaaacga atgaaagttg caaaagaaga aatcgaaatg atggctcttt acgactatgc
     4141 tgttgtaaat gatgaagtgc cgctagcagt tgaacgaatc aaaaacatta ttgccagtga
     4201 acattttcga gtagatcgcg taatcggtaa atatattaaa atgttaaagg agatgtagtc
     4261 ttatgatgtt aaaaccttca attgactcac tattagatcg tgtaaattca aaatattctc
     4321 tagtgatttt agcaagtaaa cgcgcgcatg aattggatgc aagtgcacag cctactctag
     4381 attcttttga ttcagtaaaa agcgtcggtc aagctttaga agaaatcgat gcaggaaatg
     4441 tagtcaatga tccacatcct gaattaaaac gtgaacgttt gaagatggaa gaagaggaac
     4501 gtcaagcaca aaaagagcgt gaacaacacg aattggaatc acgtatccgt gaagagcaaa
     4561 aaatgtaata aagaaaaata gagcgaacat ctaaaaagaa aagcaaaccc agctgtttcc
     4621 ttttcaaaca ttcgccactt attgtctggt agaaaaggct gtgtctgaac tttttgacac
     4681 agttcttttt tttcttatct gctgttaatt ttttgttagg atttggcaga agataagaca
     4741 aatccatcga tctctagtaa aataggcaag gaactttttg tcgcttgata agaaaagaca
     4801 aaaaaaggag gaaacgaacc atgacattaa cagctgaagt cattgtggac gtacctacta
     4861 tgcagaccga tcagccattc acttatttgg ttccctctga aatggaaaca gctttgcaag
     4921 tcgggatgcg agtagaagtc ccttttggta atggcaatcg tcatgtccaa ggatttgtta
     4981 tggctatcca aaaatcagaa gaatcagcta atccatcttt gaaggcagtt attcgtttgc
     5041 tagaccttgc tccggtggtc aatgaagaat tactttcatt agctgattat atgaaaaaaa
     5101 ttacttacgc attcaaaatc acttgtctac aaacgatgct tccgagtgtg atgaaagcag
     5161 aatatgataa attgatctat cctttggcag acacaccaga agttcaggta ttgtttgatc
     5221 agcgagaagt gatttcctgg aaggaagcag aagaagaagg cagcctttct caattgattc
     5281 gctggcgcca agagcaatta gtagatatca aatatgaagt ccatacacga aataaagtaa
     5341 aaacgattcg tttggtccgt tctttgttaa ctgaaaagca aatcgaagaa gaatgggcaa
     5401 aattacggca aaatgccaag aaacaaaaag aacttctttt gtgtttatct gaactgagcc
     5461 aagaagaacc aattgcttat ttcaaaaata aaggaatcag tactgctgtc ttgaatcaag
     5521 gaaaagaaaa gggctggctg gagtttatag aatcagaacg ttatcgcgac ccttataaag
     5581 accgagtatt tgaccaaacg acggcattgg aactgaatgc agaacaaaaa aatgctgtcg
     5641 aacaaatcat cacagcaggt cagcagcaaa aagatcaggt ctttttgctg gagggaatca
     5701 ctggcagcgg gaaaacagaa gtatatcttc aggcaatcgc tgatgttttg tcagaaaata
     5761 aaacggctat tatgttagtg cctgaaatcg cattgactcc gcaaatggtc gagcgattca
     5821 aaggacgctt cggagaatca gttgcggtct tacatagcgg actttcccaa ggtgaaaaat
     5881 atgatgaatg gcgcaagatt gagcgggaag aagcgcaagt agtagtaggc gcacgatctg
     5941 ctatttttgc cccgctgaaa aatatcgggc tgatcattat cgatgaagaa catgaatcaa
     6001 gttacaaaca agatgagaca ccgcgttatc atgcgagaga tctagcaatt tggcgcagta
     6061 aatatcatca ttgcccaata gttctaggaa gcgcgacgcc ctctttagaa tcaagagcac
     6121 gagctcaaaa aggcgtttat cagttattgc agttaaacca ccgagccaaa gcagctgcac
     6181 agcttccagc tattgagatc gtagatatgc gtgaagagtt tcagaatcat cggacctcta
     6241 ctttttctgc taatttgcag gaaaaaatac aaaatcgatt agacaaaaaa gaacaaactg
     6301 tcctactgct caatcgtcga ggatattcgt cttttgtgat gtgtagggac tgtggatttg
     6361 ttcttccatg tcctaattgc gatatctctc ttacactgca tatggataca cgttcaatgc
     6421 gctgccatta ttgcggacat gaagaaccta ttcccaatcg ctgtcctaat tgtggcggca
     6481 ataaaatccg ctactatgga acaggtactc aaaaagtaga agaggaactc cgagaacttt
     6541 atccgcaagc taggattttg cgtatggatg tagataccac tcgaagaaaa ggggcacacg
     6601 aacagatttt acaaaaattc ggtgcaaaag aagcagatat attattaggg acacaaatga
     6661 ttgctaaagg tttggacttt ccagaagtga cattagttgg ggtgttgaat gctgatacct
     6721 ccctgaattt accggatttt cgttctagtg agcatacatt ccaattgctt acccaagttt
     6781 caggacgagc agggcgagcg gaaaaagcag gcgaagtcgt gatccaaaca ttcaatcctc
     6841 aacattacgc catagaattg gctaaaaaac aaaattatga gcagttttat cagcaggaaa
     6901 tgcatgtacg gcatcgtgga ggctatcctc cttattactt tactgtgaaa ataaccgcca
     6961 gccatcctga agaacaagta gcggcaaaga aaatctttca gattgccaat cagctaaaag
     7021 aagtgttaag ccctcaaagc ttgctattag ggccaacgcc cagcatgatt ttgagagtga
     7081 aaaaccgata ttattatcaa ctaatcatca aatataaaca tgaaccgaat cttcctcatg
     7141 ttttggatga aattttgaat ggaagccaga aagaacagcg acaaggtctt tttgtggcaa
     7201 ttgacaacga accattaaac tttatttaag aaagggccaa attatgcgct atcctatatt
     7261 gatccaccct aatgataaat taaaaagaac tgctcagcca atcgatgtaa tcacggacga
     7321 gacaatcgct ttattagaca atttatatga gacaatgatt gcgaatgacg gaatagggat
     7381 cgctgctcct caagtaggac aaaacaagag aattgctgtg atcgaagtag atgaaggcga
     7441 gaaatttgaa ctgattaacc cagaaatcat cgaagcaaaa ggtgaaagcc ttgacgtaga
     7501 aggttgtctc agtattccgc atgtctatgg aacggtcaag cgagcagatg aagtgaccgt
     7561 gcgttattat gatcgagacg gagaagagat agaagtgacg gcttttggct atttagcccg
     7621 tgccttccag catgaaatcg atcacttaga cggtatcttg ttcattgaga aaatgataca
     7681 gcagatcccg gaagaagaac ttgaagaata tatggaggaa catcttgatg actaagctag
     7741 tatttatggg cacacctgct ttttctgtgc cgattcttga agggctgata gaatcaggtt
     7801 acgagatcca ggcagtggta acacagcccg accgtccagt tgggcggaaa cgagtaatta
     7861 cgccgacacc ggtaaaaaaa gcggctctga agcacggtat ccgtgtgctg cagccagaga
     7921 aaatctccgg ttcgccagaa atggaagaaa tcattgaatt ggtgcctgat gtgatcgtca
     7981 ctgcggcatt tggccaattc ttgcctgaaa agttattgca agtaccaaaa ttaggggcaa
     8041 tcaatgtcca tgcttcttta cttcctaaat atcggggagg tgcgcctgtt cattatgcca
     8101 tcatgaatgg ggagaaagaa actggtgtaa cgatcatgga aatgatcaaa aaaatggatg
     8161 caggcggaat ctatgcgcaa gaaagcatac caatcactaa acaagatgat gtagggacga
     8221 tgtttgaaaa gcttagcctt ttaggaagaa agctattgct agaaacattg cccaatattt
     8281 tggatggaca aaaaccagta cctcaagacg agtcagaagc aactttttcg ccaaacatca
     8341 cacgggaaca agaagcaatc gattggcata aaactgcaga agaaattgac aaccaagtac
     8401 gcggaatgcg tccttggcca attgcattca cgacctatga gggcatacgc tggaaattat
     8461 tatcggttga agtattggca gaaactacag acaaagcacc tggtacgatc atccggaaag
     8521 acaaaaaaaa tctatggatt gcttgcggta aacagaccgt cttagccatc aatgaattac
     8581 agccagctgg taaaggaaaa caggccgtac atgatttcct taacggaagc ggtcagcaag
     8641 ttcagattgg tcagcaggtg aaataatgaa aaaaatccct aaaaaaatga aaaaatcagt
     8701 acgatatatg gcattactga cattagagcg agtgaacaag ggtggcgcct actcgaatct
     8761 tttgttgaat gagatgatca aacaaagcga gctaaatcaa aaagatgtgg gtttatttac
     8821 agaattgatc tatggtacga ttagccatca gcgactattg gaattttacg ttgcaccatt
     8881 gatcgctaaa gcaaaaaaag tggatgactg ggtcaagatg cttctttatc tttctgtata
     8941 ccaattagaa tttttagata aagtccctgc acatgctatt ttaaacgagg cggtcgaaat
     9001 cgcaaaagta aaaggaaatc ctgggacagg gaaatttgta aatggtgtct tgaggaatta
     9061 tcagcgtcaa ggagcaccag atctgcaagg cattgctgat cctatcgaaa gactttctgt
     9121 caaaatcagc ctgcctctat ggatgacgaa acgactggtg gatcaaatcg gatacgaaga
     9181 aactgaaaag ctcggtttat cgctatatca gcctagccat gctagtgccc gagtagatac
     9241 acgccgactt tccagagatc aggccatcac tgttttgcaa gaagaagaga tcacagctga
     9301 aaaaagccaa gtcagtccat acggtatagt agcgaaaaaa gggcatttag cttctagctc
     9361 attgttccac gatggcgtca tgacgatcca agatgaaagt tcgatgcttg tagcgccgtc
     9421 tatgcaaatc gagcctgaac atcatgtctt agatgcctgt gcagcacccg gaggtaagac
     9481 gacgcatatt gcttcttttt tagatccgtc atgcggtgga gaagtggtcg ctttagatgt
     9541 acacgaacac aaagtcaaac tcattgaaga taatgcagca cgtctaggtg tctcttctgc
     9601 tgtctacgct caaaagatgg atgctagaga agtcagtaaa cagttcgaag cagagcagtt
     9661 tgatcggatt ttagtagacg cgccttgttc aggtcttggg ctgcttcggc gaaaaccaga
     9721 tattcgttat aaaaaacagc cggatgaatt agcgaattta ccaaaaatcc aattggaaat
     9781 tttagaaagc tgcgcaccaa ctttaaagtc ttccggtatt ctgacttata gcacttgtac
     9841 gatcttaaaa gaagaaaacc aagacgttgt ttctgctttc ttggaaagac accctgattt
     9901 tgagaggatt gatgttttga ccaatcctat gctacaatca tcaatcgaag agaaaatgtt
     9961 gactttgtat ccgcatcagt tttacacaga tggatttttc atttgttgtt tgcgcaaaaa
    10021 gtaatgaggt gaaaaaatgc agattgaata tcaatcagat gtaggaagac gacgtaacac
    10081 aaatcaagac tatgccagtg tatttaccag ccgttcaggt ctcaagcttg ctgtattagc
    10141 agacgggatg ggtggtcatc gtgccggaga cattgccagt cagatggcgg taacgaatct
    10201 aggcgaagct tgggaaaaac aagaagctgt tgacgatgaa aaaatcgccc aatggttcat
    10261 tcaatcgatt caagaagaaa atatgctgat ttatcagcgt ggacaagaac agccggaata
    10321 taacggtatg ggaacaacaa tcgtagcggc cgctctgtca gatgactggt tcacgattgc
    10381 caatgttggg gatagcagag cttatttgat tcgtgaagat aaaatcatgc aattgacgga
    10441 ggatcattct ttagtcaatg aactagtcaa atcaggagaa atcagcgaag aaatggcagt
    10501 gaaccatcca aggaaaaata ttttaacacg ttctgtcggc atgcctggaa cagtcgaagt
    10561 agatgtttct actcatcttt ggcaactaaa agatcgactg ctgttatgtt cagatggctt
    10621 gacgaacatg ctgtctgaag aagaaatagg aacgatcatc aatcaagaag gcccattgtc
    10681 tcataaagta actgagctga ttgatcgtgc caatgaagca ggcggagcgg ataatatcac
    10741 cgttcttctt atcgaatata aggaggacag tgtatgatcg agttgggcaa aaaattaaat
    10801 ggccgttatc aaatcactgg aaatatcggt agcggtggta tggcaaatgt atttttggcg
    10861 cacgatttaa tcttggatcg tgatgtagca gttaaagtct tacgatttga ctttcaaaat
    10921 gaccagactg cgattcgacg atttcagaga gaagctttag cagcaacaga actcgttcat
    10981 ccaaacattg ttagtgttta cgatgttggt gaagaagaca acatgcagta tctggtgatg
    11041 gaatacgtca aaggaatgga cttgaaacga tatatccaaa cacattatcc tgttccttat
    11101 gaaacagcag taaatattat gcagcagatc ctttctgcta tttctttagc tcataaccat
    11161 caaatcatcc accgagattt gaaaccccaa aacgtactga tcgataatga aggtgttgta
    11221 aaaatcacgg atttcgggat tgctatcgct ttatctgaaa catcgatcac gcagacgaat
    11281 acgatgctag gatctgttca ttatctgtcc cctgagcagg ccagaggaag tatggcaacg
    11341 aagcagtcag atatttatgc tttagggatc atcctttatg agatgttgac gggtagcgta
    11401 ccgtttgatg gtgaatctgc tgtaacaatt gctttaaaac attttcaaga tgatcttcca
    11461 tctataaaag cacttgatcc aaatgtgcca caagcactgg aaaacgtgat tttacgggca
    11521 acggctaaag aacctgcaga ccgttataag tcagccgagg aaatgtctga tgatctttct
    11581 acagtattat caccagctcg tgcaaacgaa gaaaaatggc agcctcatgt gatggataat
    11641 gaaacaaaag ttatcacacc ccttacggaa gatgcaccaa tgcctgattc attcaaatct
    11701 atgcctctgc caaaagacaa gccagaaaac gaaaccttac cagaggagga agaagttctg
    11761 cctgatccta aaaagaaagg acagaaaaaa tggtggctga tcctattagc tattcttgct
    11821 gttttaggaa tcggaacagc tgtattcttt gcttctggcg ggcgaggaga agttgttgtt
    11881 cctgatgttt ctgatctttc tgaagcttct gctagagaaa cactcgcaaa agcgaaatta
    11941 gaagtagcaa caaaaacaga agaaattgca gatgacaaaa tcgaagaagg taatgtagtg
    12001 aagacagacc cggcagcggg aacgacagtc aagcaaaaac gcgaagtcac tctgtatatt
    12061 agtagcggca cgaaaaaaat caaattagat gattacagtg gtaaaagtta tgaagatgcc
    12121 gaagcaagtt tgaaagaatt agggttttct gctgacttga ttcaaaaaga agaggaattc
    12181 agttcagagg tagaaagcgg aatgatcatt tcgcaatctc catcagaaga tacagaagtc
    12241 gatcctaaaa cagacacgat tactttcaca gtcagcaaag gaccagaaac agtgactttg
    12301 agtagtcatg taggagaaaa ctacgaaact gttcgtaatt ggcttgttac acaaggattc
    12361 aatatcaata atattagtgc tacttatgac tatagcgaca ctgtaccatc aggttcgatc
    12421 atcagtcagg atccagcctc gggtgaagtt gtagcagaag aaacttacat caattttgtc
    12481 gtaagccaag gaacagagcc taaattgaat gatatctccg gctatacaaa aagcgaagca
    12541 caaagttatt tagctagtgt cggtgcagaa tatatcgggc atgagacttc cgaatattca
    12601 aacacggtgg ataaagataa agtgattcgt acagtaccag gggcaggaac gacaattacc
    12661 aaaggaatgg ttgtgaacgt aatttactct aaaggaccag atccagcgtc ttctagttct
    12721 gaaaaagact cttcagacag ttctagcagc tcaagtagct ccagtagcac aagcaactct
    12781 tcaaaagaag aatcttccag ctccacaagt tcatcagcta gcacctcaga aagttcaaaa
    12841 taagtgctga ataaaatata tcagaaggga atgcgacatt gatttgttgc attctctttt
    12901 ttgtgtttct atctcttttg atcaattttc aagccatata ctttgagaag tttgcatgga
    12961 ataacaccta ctttcctctt cgtatggtaa aataaacaaa aacggaggtg gcacttatca
    13021 aaggacaaat cagaaaagca atcagcggtt tttactatgt atacgcaaac ggagagacct
    13081 atcaaacaag aggaagagga aatttccgga atcggaaaat cacgccgctt gtcggagatc
    13141 aggtgatctt tgagagtgaa aatcaaacag acggctatct tctggaaatc ttgccgagaa
    13201 aaaatcaatt agttcgccca ccagtcacaa atgttgatct tggaatcatt gtcacaagta
    13261 tcgtagagcc tgcattttct tataatctac ttgaccgctt tctagttacc ttggaatacg
    13321 agtcgatcga accgattatt tttttgacaa agaccgactt gtcagacgac ccagaaaaaa
    13381 tcgaagagat caagcaaacc tatactaaaa taggctatcc ggtgattgta cccaatcata
    13441 cgggtgatac gacagaactg atccgttact tcccagaacg gctatcggtc tttatgggtc
    13501 agtctggagc aggaaaatcg acgctactga atcaaatatc accggaactg aatttagcaa
    13561 caggagagat ttctgatgca cttggaagag gacggcatac aacacgtcat gtggaactga
    13621 ttcctatcta tggcgggtta gtagcagata caccaggatt tagctcgatt gactttttga
    13681 caattgaagc aggagaactg ccgagacaat ttcctgaatt tgtagaagct tctgtccatt
    13741 gcaaatttag agagtgcatg catgctaaag aacctggctg cgaagtaaaa catagagtag
    13801 aaacaggtgg tatcgcacag acacgttacg acaattattt gcaattctta caagaaatcg
    13861 aaaaaagaaa accaatgtat gcaaaaaaag aaggtaaacg aaaataggag gaaacaagaa
    13921 tgaagatagc accatccatt ttgagtgcag attttgccaa tttacaaaga gacattgagt
    13981 tagtggaaaa aggaggcgca gattacatcc acgtagatgt aatggatggc cgatttgtcc
    14041 caaatatcac attagggcca aatattgttc aggcaatccg tcctgttact aaattaccgt
    14101 tagacgtcca tctgatgatc gttgatccag aaaactacat tccagcattt gcaaaagctg
    14161 gagcagatat cattactgtc cacgtcgaag caactcctca tattcatcgg gcacttcaaa
    14221 tgacaaaaag cttaggagtt aaagctggag tagttatcaa tccaggaaca ccagtctcca
    14281 tgatcaaaca tgttttgcct ttggctgatc aagtactagt catgactgtc aatcctgggt
    14341 tcggcggaca aagctttatt gaagaaacag tagaaaaaat tacggaactc gctgatctac
    14401 gtagagagaa caattggact tatgcaatcg aagtagatgg cggaatcgtt ccagaaacag
    14461 caaaaatctg taaagatgca ggggcggatg tcttcgttgc aggatcatat atctatggag
    14521 ccgaaaatcc agtcggacaa atcgaacgat taaaagaggc attagactaa atgaaaattt
    14581 tattagctgc tggaggtaat ccgcaaaact ggccgcagtc tctgctgtcc aaatacgatg
    14641 cctatgtcgg gatagaccgt ggtgcgctgt acctgcttca agcaggctac ccagtagatc
    14701 tggcagtggg tgattttgat tcattgtcga acgaagagca gaaggaagta tttgcaaaag
    14761 ccaaagagta tacccgttcc cctgcagaaa aagacgatac agatacacag ttaggtatct
    14821 taaaaacatt tgaacgttac ccgcaagcgg aagtcacttt gattggagct acgggcggaa
    14881 gattggatca tcttttagcc aatctctggt taggtatgga acctcgcttc aaaccctttg
    14941 cccatcagct tattatcaga gattgtcaaa attatttgtc ttattatttg cctggagagc
    15001 atatagtaag acagatacca gatatgaagt atttagctta ttgctgcttg actcccgtct
    15061 cgaatcttac attaaaaggc agcaagtatg tattggaaaa taaagaagta gaatatccga
    15121 catcttatgc aagtaacgaa tttctttcag ataccgcaga attttctttt tcagatggta
    15181 tgattgcagt tattcaaagc aaagattgat ccataaacaa ataaaaaaat ttgtctttca
    15241 ggctaaaaat gacagaatct attatttgct aaaaaaaagc ttaagaggag caacttacct
    15301 tttgtttcac ggcatttagt gataaaattg aacacaagag tctaattaat tcgaggtgtc
    15361 tttgtgaaga agttcaatcc taataaaaat attattatta ccttggttat tgttatcatc
    15421 gtggtgacga tactaagtgt gaccgctgcg cagcgagcga atgaaggaaa gcccaatttc
    15481 ttccaatcca tcgttaatga cagtgttgca atggttgata aaacaatctc tgccccagta
    15541 cggtgggtac gaaatggagt agattctgtc cataatcttt tttcaactta tgccgaaaat
    15601 gagcggctaa aagaaaaaat cgatagctat gatgacgttt cacagcaaaa caagaattac
    15661 gagagagaaa tcgaagcgtt gaaaaaagaa cttgagttga atcagacgct gactagttac
    15721 gaaaaagtaa cggcaaatgt cattactcgt tcgcctgaca gttggcagga catgctgatc
    15781 gtagacaagg gttctaaaga cggtatcgaa gcaaatatgg cagtcatgtc gcaaaaagga
    15841 ttgattggcc gagtgatcga ggtcaatacg gcttcgtcta aaatcgaatt actgtcatcc
    15901 tctaatgaaa gctccaatca ttttccagta cgggtatctt cggctaatgg cgaagcgttt
    15961 ggtttgctta aaaactatga tgaaaagctc catgccttag tggtgaccca attaactggt
    16021 gatacggata tcaaagaagg ggatgttgtc cagacatccg gtcttggagg gaattctcca
    16081 gctaacttgc cgatcggtac ggttattaaa acgaaaccag atagttatgg gctggatcgg
    16141 gaagtttatg tgaaacctta tgcagaaatg tatgacgtgt cagttgtgac gattgtccaa
    16201 cggctagcgg aggattagtc catgttgaag aaagaaacaa tgaagtacta tctgccaatc
    16261 gttttgttct ttttgatgtt gatagatggt catttaacaa gaatgctagg ggagtggtcg
    16321 aaaggcacct atatgtcaaa tgcccacttt ctgatattgg cattattatg ttgcagtatg
    16381 gcgtttgaaa aacgttattt actgattacc acgattgttc tcggggctat ctatgacgct
    16441 tactatattg gcgttatcgg tatctatgca gtagctctcc ctttaattgt atggttgatg
    16501 tatgtaatga aagacgttat ccatgtcaac atctttactg aatttttcag tatgatcatc
    16561 tttgtcacgg gttatgaatt gtttacgatg gtggtccagt tgatttttaa attagcagta
    16621 gtaaataaca cgtattttat tacaaggttt ttaggaccta cactgctgtt gaacatgatt
    16681 atatttgtat tattcatttt tccctttaag aaattattca gtgatgaata atttcttttt
    16741 ttatttttag gtcgaaagcg aaaaagaatc gttgttatct taagaaatgc tagacttaac
    16801 gcggcttttt catgatcatt ttctgaaacg aacagttttg tcatttttaa aagttgtttt
    16861 gaaacattt
//
LOCUS       DAYYME010000057        16739 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000057 DAYYME010000000
VERSION     DAYYME010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.057"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..781
                     /locus_tag="ACHBFW_001530"
     CDS             29..781
                     /locus_tag="ACHBFW_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9317838.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            778..1692
                     /gene="pfkB"
                     /locus_tag="ACHBFW_001531"
     CDS             778..1692
                     /gene="pfkB"
                     /locus_tag="ACHBFW_001531"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM9317839.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1710..3641
                     /locus_tag="ACHBFW_001532"
     CDS             1710..3641
                     /locus_tag="ACHBFW_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM9317840.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3752..5311)
                     /locus_tag="ACHBFW_001533"
     CDS             complement(3752..5311)
                     /locus_tag="ACHBFW_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFM9317841.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5466..7700
                     /gene="pcrA"
                     /locus_tag="ACHBFW_001534"
     CDS             5466..7700
                     /gene="pcrA"
                     /locus_tag="ACHBFW_001534"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFM9317842.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7717..9753
                     /gene="ligA"
                     /locus_tag="ACHBFW_001535"
     CDS             7717..9753
                     /gene="ligA"
                     /locus_tag="ACHBFW_001535"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFM9317843.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9859..10164
                     /gene="gatC"
                     /locus_tag="ACHBFW_001536"
     CDS             9859..10164
                     /gene="gatC"
                     /locus_tag="ACHBFW_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFM9317844.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10164..11636
                     /gene="gatA"
                     /locus_tag="ACHBFW_001537"
     CDS             10164..11636
                     /gene="gatA"
                     /locus_tag="ACHBFW_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFM9317845.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11633..13063
                     /gene="gatB"
                     /locus_tag="ACHBFW_001538"
     CDS             11633..13063
                     /gene="gatB"
                     /locus_tag="ACHBFW_001538"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFM9317846.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13073..14128
                     /locus_tag="ACHBFW_001539"
     CDS             13073..14128
                     /locus_tag="ACHBFW_001539"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM9317847.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14239..15615
                     /gene="rlmD"
                     /locus_tag="ACHBFW_001540"
     CDS             14239..15615
                     /gene="rlmD"
                     /locus_tag="ACHBFW_001540"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM9317848.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIECV
                     GLLTRVER"
     gene            15824..16660
                     /locus_tag="ACHBFW_001541"
     CDS             15824..16660
                     /locus_tag="ACHBFW_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM9317849.1"
                     /translation="MKQIFIFSDTQKDNISYFEREPAILVSMSRSDLPYLKEQDFSNY
                     PAVYVLIGGNKRYVGQAAGQSISLRLSQHFLKEYKAWVESVLFFTRADGKMSKADTDY
                     LEKRLIQDFQEKSDYEMMNSTAGNTSYIDKLQKAKSDQLYDTVFEIIDEIANIDLFGT
                     SEDSETQIYEFSSSGQYEIEYDGKKISSKSARGLLVDFVSQLFADGNYNNQLQELIVD
                     ETPSFAIILGRKPSMYNGRPNSARVADGVWVYANFNKKNVRIKIDKLARQLGIPVKIK
                     WN"
BASE COUNT         5648 a         3029 c         3679 g         4383 t
ORIGIN      
        1 tattatttgt attgaggtga tcgtgaaaat gcttacagag gaacgtagac aaaaaatcct
       61 agacttgctt gaacagcaga aaatcgtgaa atcacaagat cttgtaaacc tttttaatgc
      121 ttctgaatca acgataagac gtgatttaca agagctggag aatagtgggt ttctagaaag
      181 agttcatggc ggagctaaga aagagcaatt attgaatttt gagcagaata tgagtgaaaa
      241 atcactcaaa aacgttcacg aaaaacagca aatcgccaaa tcggcagctg aaaccattaa
      301 tgacgaagat gtcatttatt tagacgctgg ctctactacg ttagaaatga ttccttttct
      361 gcaaggaaaa cagatcacag tagtgacaaa ttccgttcaa catgcggcaa atttagttga
      421 tttggagctt gctaccatta ttttaggtgg aacgataaag ctggcaacta atgctgtttt
      481 aggatctaat gcgatagaac aactaaaaaa ttttcatttt aataaagcat ttatgggaat
      541 gaacggtgcc catcttgaac aaggattcac gactccggac ccagaagagg cagctgttaa
      601 acgtttagcc atgaaaaatt ccgaagagac atttgtattg attgatcata ccaaattcaa
      661 ccagttgtcc ttcacctctg ttgcaccttt ggaagcagcc acgatcataa ccgagcgttg
      721 tccattagaa ttcgttaatg aattccgtga aaagacaaca attaaggagg caatacaatg
      781 atttacacag taacacttaa tccatcaatt gattatatcg tacgcgtaga tgggttaaaa
      841 ctaggcgatt tgaaccgaat gaaggaagac ttcaagttac ctggaggaaa agggatcaat
      901 gtttcccgta ttttgaaacg gatcgattct caatctacag cactagggtt tttaggaggg
      961 ttcactggcg aatttatttc gaagtggctt gaaaaagaac aaatccagtc tttgttcaca
     1021 accgtaaaag aagatacaag aatcaatatc aaactaaaat cagatactga aactgaaatt
     1081 aacggtttgg gcccatctat ttcagatgaa gaaatcgatg agttgaagca agcattagga
     1141 acagtaggac aaggagatat cgtcgtattg tcaggaagcg cgccggctag cctgcgaaaa
     1201 ggattttatc aagaactgat tgatatcatt cgagaaaaag gtgcagattt cgtgattgat
     1261 acaacaggtg atgacctaaa aaatgcactt gttaagaaac cattgttgat caaaccaaac
     1321 aatcacgaat tagctgagct ttacaataca accttccaat ctgtagaaga aatcattccg
     1381 tatggtaaaa aattgctagc tgaaggggca cagaatgtgc tgatctcaat ggctggagac
     1441 ggtgctttgc tcttcacgga acaaggaact tataaaacga atgtattagt tcgcccattg
     1501 aaaaattcag taggtgcagg ggattcaatg attgccggct ttattggggc ttacagccgt
     1561 acgaaagatc caatcgaagc ctttaaatgg ggagttgctt gcggaagcgc aacagctttt
     1621 tctgatgact tggcagctcg cgaattgatt gatgagttgc tgccagaagt tgaaatcaca
     1681 aaaattgatt agtttcaagg aggataatca tgaatatcaa ggatttaatg gtcaaagaag
     1741 cgatgatcat ggatttgcaa gcgactgata aaaaaggcgc aatcgatgaa atggtccaaa
     1801 aaatgtttga tgccggccgg atttccgata tcgaaacata taaagaagga attctagccc
     1861 gcgaagcaca aacttctaca ggtcttggtg atggaatcgc catgcctcac gcgaaaaaca
     1921 gtgcagttaa agaagcaaca gttttattcg caaaaagcca aaaaggagta gattatgaag
     1981 ccttagacgg acagccaact tatctgtttt tcatgatcgc agctccagaa ggcgcaaacg
     2041 acacacattt gcaagcatta gcagcactat ctcgattgct gattgatgca gatttcgtag
     2101 gaaaattaaa agaagcaaat acaccagaag aagtccaaga cttgttccaa acagctgaag
     2161 agcaaaaaga agcagaagaa aaagcagaac aagaaacaca agctgcagaa gcttctgctc
     2221 atccagaaag aaaattcgtt gttgccgtta cagcttgtcc aacagggatc gctcacactt
     2281 acatggcaga agatgctctg aagaaaaaag cgaaagaaat gggtgtggag atcaaagtcg
     2341 aaacgaacgg ttcagaagga atcaaaaacc gattgactgc tgaagacatc gcccgtgcag
     2401 aaggtgtaat cgttgcagca gacaaaaaag tagagatgaa tcgctttgac ggaaaagaac
     2461 tgatcaatcg tcctgtcagt gatggtatcc gtaaaccaga agagctgatc aatctagccc
     2521 ttagtggtac tgcaccagta ttccatggtg atggaaaaga aagcagttca gaagaaggaa
     2581 atgcggatgg tactatcgga caaagaattt acaaagattt gatgaacggc gtatcacaca
     2641 tgttaccttt cgttattggt ggaggaatcg ctattgcgct ttctttcatg atcgaccaat
     2701 ttattggcgt gccacatgat caattatcaa atctagggaa ttacaatcaa gctgctagct
     2761 ggttcaacca aatcggacaa gctgcatttg gtttcatgct gccggtatta gcaggattta
     2821 ttgcttcaag tatcggtgac cgccctggtt tgatcgttgg atttgctgct ggtgcattag
     2881 ctaatacagg cggtgcagga ttcttaggtg cactgattgg tggattctta gctggttatg
     2941 tcatcgtatt tttacgtaaa ttattcaaga acttaccaaa atcattggat ggaattaaaa
     3001 cgatcctctt ttatccagtg tttggcttac tgattactgg tttcttgatg ttgctggtca
     3061 atgtccctat gaaagcaatc aatgatgcat tgaacggttt cttgacaagt atgagtggtt
     3121 cgaatgctgc tttacttggt gcattattag ctggaatgat ggcggcagat ttaggcggtc
     3181 caatcaacaa agccgcttat gtgtttggta cagcaacttt agctacgact gttgcaactg
     3241 gcggaagtgt cgtgatggct tctgttatgg ctggaggaat ggtacctcca ttggcaattt
     3301 tcgttgctac acgtttgttt aagaataaat tcagcaaaac agatcaagat gctggtttga
     3361 cgaacatcgt gatgggacta tcgtttgtca cggaaggatc gattccattt gcagcagctg
     3421 acccgattcg tgccattcca agttttatta ttggttctgc tttaactggc ggattagtcg
     3481 gagctttcgg gatcaaatta ctggctcctc atggcgggat tttcgttgta ttcttactga
     3541 gtcacccggt catgtatttg gtgtttattg ctatcggagc agtcgtatct ggtgtgattt
     3601 atggtttatt gagaaaagct cctgcagcag cagaagcata gaaataaaaa agaagaggct
     3661 ggtttttccc agtctcttct ttttttatag aatcatatag aatcaagtag tgtagatgaa
     3721 tagttgttgt aaaatagttt ttccatgttc gttaaaatga ttgcttcaaa tttgtgatag
     3781 aagatttagc ctcgatttcc tgcttgattc ttggcaagtt tgcttcatca gtcaagataa
     3841 ccaaaatatc tcctacattc atgatcgtat caccgtgagt caagatttcc tgatttcctc
     3901 tgcgtataga gattagcagc atatttttag gccaattgaa gtctcgtacc attaatccgt
     3961 ctaaagaact ctctgccgtt actggaaaat caaaagttgt ccttgcacct tttagtgagg
     4021 cggtctgagt atttccaagt agacgttcca ataatgattc gtaaattgga ttaccgccga
     4081 gaaggtcatt gacgatataa gcaacaagag cagccaatcc taaaggcatc agatgattaa
     4141 gagagccaac catttctgtc actaaaataa ttgctgtcag cggtgctttt ccaatcgctg
     4201 tgaaataacc agccatggca ataacgacaa aatccttgat gtagattgga tccatcccga
     4261 aatcaaccat aatgtttcca taaaagctac cgattaaagc ccccaatgtc aatatcggaa
     4321 ggaaaatccc ccctggaaga ccaccaccgt aagaaatcat ggaaaaaacg aatcgaagaa
     4381 caaacaaacc gataatcata atgattggca aggaagcatg tccaaaagct aaaacgattt
     4441 gattgcctcc gcctagagta ctaggccata aataaccaat tggcaaaatc agcaagaaag
     4501 gaacgaaccc gtgaaaataa gctggtaaag gcaatcggtt gtaaaatttt ggcaaagcca
     4561 gcaagacctt ctgataaata aaacctaaga tgcctagaag tactcctaag atcactaagg
     4621 aaccataata tttcaacggc aaagaaggaa ggtcacctag atataaaaca ggtctcaagc
     4681 cgaaaaagta aagagagacg aagttagcag tgattgctga agcaaatgat gtcagccaaa
     4741 caagtggaga aaaggagtga tggatttctt ctaaaacaaa gagtaatcct gcgataggag
     4801 cattgaatgc tgctgcaagt ccgctacttg caccgctaga aattaaaatc ttttcttctg
     4861 aagatggact ccgaaacaat tgtccagctc cttgtccaat ggatgcgcca agctgtatag
     4921 aaggtccttc tctacctaga aaaaggcccg caccaactga aagtacaccg ccaatgaact
     4981 ttttccataa aacagagaac cagttcattg ataattcccc gcgaagctgt ccctctactt
     5041 gtggaatccc gcttcccttg atatccggtt catttttgac cagataaccc acgaaaaatg
     5101 ctgcaaggat gcagcaaata gctaggccaa ttaagtaaat cggctgttcc cgactcattt
     5161 catagatact tcgaactaaa tcgcttagct tttcgataga taatcgaaac aggctaacga
     5221 cgattcctgt cagcagtcca acaaatattc cttttagaat aaaatatatc tgagtcttat
     5281 ctaatttctt gatttttcct tttttttcca ttcaaacgcc tcgctttctt ttaatttagc
     5341 gcgaaatcaa cgaattgaca aggaaaaatc cataaaaaga aacaaatgat tttgaactag
     5401 ctagtttaat gaaagccaag aaaatgctat aattaacaag attgataaaa gaaaggatgt
     5461 tgttaatgtc atccaaagct gaattattaa acgggatgaa cccccgtcag aaagaagctg
     5521 ttcttcacac tgacggacca ttactgctaa tggcaggtgc aggaagtgga aagacgagag
     5581 tgctgacaca ccgcattgcc tatttgattg aggaaaaaga agtcaatcct tggaacatcc
     5641 tagcgattac ttttaccaat aaggctgcaa aagaaatgaa agaacgtgtc aatgcgattc
     5701 tcgcatctgg aggcgaagat gtctgggtat cgacttttca ctcgatgtgt gtgcgtattt
     5761 taagaagaga tgttgatttt atcgggtata accggaactt tacaatcatc gattcttctg
     5821 aacagctgac cttgatgaaa cggatactaa aagaactgaa cattgatccg aaaaaatatg
     5881 atcctcgaag tatattagga acaatctctc aagcaaaaaa cagcctgcag acccctcagg
     5941 attttgcaaa aatgcaaggc agttattacg aagagattgc ggcaaaatgt tatgctgctt
     6001 accaaaaaga gcttcaatat aatcaatgta tggactttga cgacctgatc atgaatacga
     6061 ttcgattatt tgaagaacat cctgattcac tcacatatta tcaaaacaaa ttccattata
     6121 ttcatgtaga tgagtaccaa gatacaaatc acgcacagta tacgctagtc aatttattgg
     6181 caggtagatt taggaacctg tgcgtcgtgg gagatgcaga tcaaagtatt tacggctggc
     6241 gcggtgcaga tatgcagaac atcttggatt ttgaaaaaga ttatccagat gctgcagtaa
     6301 ttttgctaga acaaaactat cgctccacga aaaacatcct tagcgcagca aaccaagtca
     6361 ttgaaaacaa tagcaatcga aaaccaaaaa acttgtggac agaaaacaaa gaaggaaaca
     6421 agatcactta ctaccgtgca gataatgaac gagacgaaac ccgttttatc gtagatcgga
     6481 tgcaagaaga gattcgaagc aatcatcgga attacggcga ctttgccatc ttgtaccgga
     6541 cgaacgcaca atctcgtgta atggaagaaa ctctgttgaa agcaaatatt ccttataaaa
     6601 tggttggagg gcacaagttc tacgatcgta aagaaatcaa agatatttta gcgtatttga
     6661 atgtgctggc aaatcctcaa gattcaatca gttttgagcg tatcgttaat tcgcccaaac
     6721 gaggcatcgg accaggatct atcgaaaaac tacgcagttt tgcttctctt catgaatggc
     6781 cactgcttga agcagcgcaa aatgtggatc tggcaaatat cggtggaaaa gcaggacagc
     6841 aattaggtgc atttggagag atgatccaag aagtcactca gatgatccca tacttgacag
     6901 tgacagaatt gacgaaagag gttttggatc gtagcggata tctagaagac ttaaaaatcc
     6961 aaaatacatt agaagcacaa gcacgcatcg aaaacttgga ggaattttta acggtcacac
     7021 aagaattcga caaacaattc gaacagcaaa atgaagaaga cgctgatgca ccagaagaaa
     7081 aactaacggt cttcttaaat gatctggcac ttgtttccga tattgacaat ttagaagaag
     7141 atgcttccca agtgacattg atgactttgc atgcagccaa aggattagaa ttcccagttg
     7201 tattcctgat tggtctagaa gagggggttt ttcctttatc tcgagcattg atggaagaaa
     7261 gcgaactgga agaagaacgc cgtctcgcat atgtggggat cacgcgagca gaagaagcgt
     7321 tgtatttaac aaatgctttc tccagaacat tatatggacg gacgcaatat aaccgacctt
     7381 ctcgttttgt agaagaaatc gatcaggagt tactggaaat cgaaggcatg cgtcctacgc
     7441 ctaaaaaaac acctgtattc gctaaaaaaa cagcatatag ctataaacag ccagagacag
     7501 cggttgttcc ctctaaaagt gctactggag gggaaaataa cagctggaaa cctggagata
     7561 aagtcaaaca caaaaaatgg ggtctaggaa cagtcgtccg agtaagcggg acgtcgaagg
     7621 atctagaact agatgtggct ttcccaagtc aaggagtaaa acgtcttttg gctgcctttg
     7681 cgccaattga aaaagcttag tagtggaggg taaatgatgg aacatgaaat gacgattgac
     7741 gaagctgcta aacgagcaga agaacttcgt acaagactga accaatggtc tcgcgaatac
     7801 tacgtagaag ataaaccaac agtggaagac tatgtttatg ataaagaata cgcagaatta
     7861 gtagcaattg aggaacaata tcctgatttg atcacgtctg attctcccac tcaacgagta
     7921 ggcggaaaag tattagaagg ttttgaaaaa gtcactcatg atattccgct ttacagtttg
     7981 aacgacgttt tcagcaaaga agagctgata gcttttgatc aacgtgtaca aaaagcagtg
     8041 ggacgtgtag ttgactattg ctgtgaacta aaaatagatg gactttccgt ttctttgcgt
     8101 tatgaagatg gaaatttcgt gagaggtgcg actcgcgggg atggaactgt aggagaaaac
     8161 atcactgaaa acctaaaaac tgtccggtcg gtcccaatca aattaaaaga accaatgaac
     8221 atcgaagtaa gaggcgaatg tttcatgcca aaacggtcat ttgtccaatt gaaccaagac
     8281 agagaagcag agggaaaaga tatctttgcg aatcctcgga acgccgcagc tggttctttg
     8341 cgtcagttag attctaaaat cacagcgaaa agaaatctgg acactttttt atacacagta
     8401 gctgatttcg gtcctatgca agcaaaaacc cagtatgatg cgctagaaga gctagaaaaa
     8461 attggattcc acacgaatcg ggaaaaacgt ctatgtcatt cgattgatga agtgtggtca
     8521 tacattgaag aatatcatga caaacgtgtg gatttgccct atgaaatcga tggaatcgtc
     8581 atcaaagtga atgaattctc gctacaagat caacttggtt ttactgtcaa agcaccgaga
     8641 tgggcagcag cttacaagtt tccgccagaa gaagttgaaa cgctaatcga aaacatcgaa
     8701 tggacagtcg gtcgaacagg ggtagtaacg ccaacagcta tcatgacacc agtccgagta
     8761 gcaggaacga cagtaagccg tgccagcctg cataacggtg attatattaa gttgaaggat
     8821 atccgcttga aagatacagt tctgatttat aaagcgggag acatcatccc agaagtttcg
     8881 caagtcgtgt tagataaacg acctaaagac agcgaagaat accaattgcc tacacattgt
     8941 ccagtttgtg gaagtgaact tgtccatttg gatgaagaag tagccttacg gtgcatcaat
     9001 cctaaatgcc ctgctcaaat gaaagaaggt ttgaaccact ttgtttcaag aaatgcgatg
     9061 aatatcgatg ggttggggcc gcgcgtacta gaacagatgt atgataaaaa actagtagca
     9121 gatgttgctg atctttacaa attgacagaa gaagagttat tgacgttgga caaaataaaa
     9181 gaaaaatcag caaataatat tttgacagca atcgacaaca gcaaagataa ttcggtcgag
     9241 cggttgattt tcgggttagg aatccgacat gtcggagcga aagcagcaaa aattttagca
     9301 gaacatttcg gtgatctaga aacattgagt aaaagtgact atgagtcgat tattgcgctt
     9361 gatacgatcg gtgatatcat tgccgacagt gtagtcactt atttcagtaa cgaagaagtc
     9421 catgaattga tgaatgaatt aaaacaagca ggcgtcaatt tcgaatacaa agggcttcga
     9481 aatgcccaat tgcaagaagt cgagtctcct tttaaagaaa aaacagtagt ccttactggt
     9541 aaactaacac gatttactcg tgaagaagca aaagagacga tcgaaaatct tggcgggaaa
     9601 gtgactggta gcgtatcgaa aaaaacggat atcgtggtag caggagaaga tgctggcagc
     9661 aagctgacca aagcacaaga attaggaatc gaagtttgga cagaagatca aatggcagat
     9721 gcattggcta aaagtcgctc agtagaagaa tgaaaaatga gttttttcag acaaatttca
     9781 ataaacaatt tcagacacgg cactcttgtg ttaaaataga gagcaaagtt tttaactcgt
     9841 ttagaaagaa ggggtagtat ggcgattagt gaagaacaag tgaaacacgt tgccaaatta
     9901 gcaaaacttg cgttcgcaga tgaagaactt gcaagtttta ccgaccaatt aggtaaaatc
     9961 atcgatatgg ttgaaatgct ggaagaagta gatacagaag gtgttccatt tacgtcgaat
    10021 gtggttgaaa cagtaaacgt gatgcgacaa gatcgggcgc aagctggatg gaaccgcgat
    10081 gaattattga aaaatgtgcc ggaaacagaa gacggcttta tcaaagtacc cgcaatcatc
    10141 gataatgggg aggctggcgc ataatgacag aattacacaa tcgttctcta gaagaactgc
    10201 atcatatgct tgtatcgaaa gaaatcactg tacaagattt gacaaaagca acatttgaac
    10261 gaatcaaaga aacagagcct gaggtcgaag cattcattac attgaacgaa gaaaaagcat
    10321 tggaacaggc aaaagcttta gatgaaaaag ggattgatga aagcaacgtc cttgctggta
    10381 ttccaattgg tatcaaggat aatatcgtaa caaaagatat cttgacaacc gctgcatcta
    10441 agatccttta caattttgaa ccaatttatg atgcaacggt catggataaa atctatcaag
    10501 cagatatgat ccctgttggt aagctaaaca tggatgaatt tgctatgggt ggaagtacag
    10561 aaacttctta tttcaagaaa acaaaaaatg cttgggaccc aacaaaagta cctggtggct
    10621 cttctggtgg ttctgcagca gcagttgcag ctggacaaat tccagtttct ttaggtagtg
    10681 atactggggg aagtatccgc caaccagcag cttttaacgg catcgtaggg atgaaaccta
    10741 cttatggccg tgtctcacgt tttggtttga ttgcctttgc ttcatctctt gaccaaatcg
    10801 gaccaatgac ccgtacggtg aaagataatg cattagcatt gacagctatc agcggttatg
    10861 acgaaaaaga cggtacttct tcaggcgttt cagttcctga ttttgcagag ggactgacag
    10921 gcgacatcaa aggaatgaag attgctttac ctaaagaata cttaggcgaa ggtgtggccc
    10981 ctggcgtaaa agaagctgta ttgaaagcag cagaaacatt ccgttcatta ggtgctgttg
    11041 tggaagaagt cagcttgcct cattcaaaat acggtgtggc tgtttattat atcattgcct
    11101 catcagaagc aagttccaac ttgcaacgat tcgatggtat ccgctacggt tatcgctcag
    11161 aaaatgtgaa atcattagat gatgtatatg tcaactcacg ttcagaagga ttcggtgaag
    11221 aagtaaaacg ccgtatcatg ttaggtacat tctccttaag tgctggttat tatgacgctt
    11281 acttcaaaaa agcgggacaa gtccgtacat tgatcaaacg agattttgaa aaagtctttg
    11341 cagattatga cttgatcatc gggccatctt caccaacagt tgcattcgga atcggtgaga
    11401 acattaacga tccaatcaca atgtacatga gtgacatctt aacgatccct gtcaacttgg
    11461 ctggacttcc aggtatgtct gtaccggcag gtttatcaga aggattacca gttggattgc
    11521 agatcatcgg taattatttt gatgaaaaaa cgatgtatca agcggcatat gcgtttgaac
    11581 aagcaacaga attccataaa caacaaccag ctattttagg agggaaagac gaatgaattt
    11641 tgaaacagtc attggactag aagtccacgt cgagttaaaa accaactcaa aaattttctc
    11701 tccagcgcca gctcattttg gtgcagaacc aaacagcaat acaaacgtga tcgactgggg
    11761 ttatccaggc gtgctgccag taatgaataa agctgcgctg gaattcggga tgaaagcagc
    11821 tttggcatta aactgtgaaa tctctaaaga tactcatttt gaccggaaaa attactttta
    11881 cccagataat ccaaaagcat atcaaatttc ccaatttgat caaccaatcg gtcatgacgg
    11941 atggatcgaa atcgaagtag aaggtaaaaa gaaaaaaatc cggatcgaac gtgtccattt
    12001 agaagaagat gctggaaaaa acatgcatgg tattggcggt tattcttacg ttgatttgaa
    12061 ccgacaaggc acgcctttga tcgaaatcgt ttctgaagct gatatgcgtt caccagaaga
    12121 agcatacgct tatctagaag cattgcgatc aattatccaa tttacagaag tcagcgatgt
    12181 gaaaatggaa gaaggctcta tgcgctgtga tgccaatatt tctcttcgcc cttatggaca
    12241 agaagagttc ggtacgaaag cagaattgaa aaacttgaac tctttatctt tcgtgaaaaa
    12301 aggtttagct tttgaagaaa aacgtcaagc aaaagttctt ctttcaggag gagaaatcca
    12361 acaagaaaca cgccgttttg acgaaacaac gaataaaaca gttttgatgc gtgtcaaaga
    12421 agggtctagt gattatcgtt acttcccaga gcctgatatc ccgaaattcg tgatcgatga
    12481 cgaatggatt caaaaagtaa aagctagttt gcctgagatg ccagcttctc gtcgcgaacg
    12541 ctatatccgt gagtttggac ttccagagta tgacgcaatg gtattgacac ttacaaaaga
    12601 aatgtctgat ttttttgaag ctgttttgga aaatggtgca gatgccaagc aagcatctaa
    12661 ctggttgatg ggtgaagttt ctgcttactt gaacagtgag aaactggaat tagctgagac
    12721 aaaattgaca cctgagaact tagcaggaat gattcgtttg atcgccgatg gaacgatcag
    12781 ctcaaaaatc gccaaaaaag tcttccgtga attgatcaca aatggcggag aagcccaaga
    12841 agtagttgaa aaaaatggtt gggtgcaatt atctgatccg aacaaactac tgccaatcat
    12901 taatgaaatt ttggataaca accaacaatc tgtcgatgat ttcaaaaatg gaaaagatcg
    12961 tgcggttggc ttcctagtcg gtcaaatcat gaaagcaact aaaggacaag caaatcctgg
    13021 tgtcgtgaat aagctactta aagatgaatt agcgaaacga tagaggctga aaatgaaaaa
    13081 agcacgcgta atttataacc ccacctcggg aaaagaatta ttgaaaaaaa atttggcgga
    13141 tatcctacag gcattagaag aagcaggatt tgaagccagc gcctatgcaa ctacaccaga
    13201 accagattca gccaagaatg aagcatgtag agtcgcagaa ctaggttttg atctagtagt
    13261 ggcagctggt ggcgatggca caatcaacga agtggttaat ggaatcgctc cgcttgaaca
    13321 ccgtcctaaa atggccatca tcccagctgg tacaacgaac gactatgctc gagcattaaa
    13381 aattcctaga gataatatta aagcagcagc agaagtgata aaaaaacacc aaaccgtaaa
    13441 aatggacatc ggcctttctg ataacagtta cttcatcaat atcgctgcag gtggctattt
    13501 gacagaattg acttatgaag ttccatcaga actaaagagt atttttggtt atcttgctta
    13561 tcttgcgaaa ggagcagaga tgctccctcg agtaaagcca atcaaaatgc gcttgaagta
    13621 tgatgaaggg gaatatatcg gaaatgcttc gatgtttttc ctagggctga cgaactctgt
    13681 tggaggattc gaacagatcg cgccagatgc caaattggat gatgggaagt tttctttaat
    13741 cattgtgaag acagcaaata tctttgagat cttgcatatt gcagcactca tgctgaatgg
    13801 cggtcgacat gtcgatgatc caagaataat ctataccaaa acaagcagtt tgttcgttga
    13861 aacagaagat gatatcaatc gaccagtaat gatcaactta gatggtgaat acggcgggga
    13921 agcaccgatg catttccaaa atcttcatca acacattgag ttctttgcca ataccgatca
    13981 gattcctgat gaagcaatca caggtacaga tgaagaagaa ttagaggttg tcagcaaaga
    14041 attcgtaaaa gaagtcgaac gtttaacgga tgaagatatc gacggtgacg gaaaaatagc
    14101 agacgaaaca cctaatcaaa agaattaatc aaaagccggt gctgaacgta gtaaggtcag
    14161 tgccggtttt agttgtgtaa ttagctgatt tatagtagag ttcagtagga ttgaaaaata
    14221 ggcttaaagg agcaaaatat gacagaacag caaatagtaa agaaaaacga acgatataca
    14281 gtcgaaatcg tcgacttgag ttatgaagga atgggggtag caaaaatcga tggttatcca
    14341 ttatttatcg aaaatgcact ccctggagaa gaagtagaaa tactagtagt aaaagcaggc
    14401 aataagtttg gatatggaaa agtcgaaaca tttattacac aatcacctga tcgtcagcct
    14461 gtagaaaaca atgcattact tcgcataggt atcgcacctt tagcccattt gaagtatgaa
    14521 caacagcttg tcttcaaaca aaaacaagta gagcatgtaa tggcgaagat tgctaagatg
    14581 ccagaagttc ctgtacttcc aacggtcgga atggcagaac cattcggcta ccgtaataaa
    14641 gcacagatcc ctgttcgccg tatcgatgga cagttagcca ccggttttta caaaaagaat
    14701 agccatgatc tagtccctat cgaagatttt tatatccaag atcctgtcat cgatcaagca
    14761 atcaaagtag tcagagatat cttgcaacgg ttccaagtac gaggatataa tgaagaaaaa
    14821 aatgaaggac agcttcgtca catcattatc cgtcgtggtc attattccca tgaaatgatg
    14881 gtcgttttag ttactcgtaa agaaaaattc tttaaatcca aggaaattgc tgaagctatt
    14941 catgaagaat tgccagaagt ggtttcggtc atccaaaata tcaatgaaga aaaaacaaat
    15001 gtgattttag gagacaaaga aaaagtttta tatggcagag actatatcga agatcagcta
    15061 ttaggaaaaa cctatcgaat ctcagcaaag tcattttatc aagtaaatac tcaacaagca
    15121 gagtatctgt accaaacagc catcgagttt gccgagttag ataaaaaaga cattgtgatc
    15181 gacgcttatt caggaatagg tacgattggc ttgtctttag cagacagagt ggataaggtg
    15241 tatgggatgg aagttattcc agaagcaatt gaagatgcac agttcaatgc tttaaataat
    15301 aaaatcgaaa atgttcatta tgaagtcgga aaagcagaaa aagttatgaa aaaatggcaa
    15361 gaaaaaggga tcaaaccatc tgtgatcgta gttgaccctc cacgaaaagg attagatcat
    15421 cattttattg aatcagcaat cgaaatgtca ccagaacgaa tcgtctatat ctcatgtaat
    15481 ccggcaactt ttgcaagaga tgctaagtta tttgcagaag caggttatgc cgtgaagaaa
    15541 gttcagcctg tagatctttt cccgcaaacc catcacatcg agtgtgtagg actgcttaca
    15601 cgtgtggaac gttgatatga caaggattag agcgattcgg gatggtttcc ggattgctct
    15661 tttttcgtgt tgaaatgacg atttttgggt gagcgtagag ttgatggaaa tgctatggag
    15721 ctttgagttg ataggagtta caccgtagac ttgataggtt agatgatttt gataacggtt
    15781 acgtgatata atatattatc gagttttcaa gggagacaaa cttatgaagc aaatttttat
    15841 attttcagat acacagaaag ataatatttc ttattttgaa cgcgagccag caatcttggt
    15901 ttcaatgagt cgtagtgatt tgccgtattt gaaggaacag gatttttcaa attatccagc
    15961 tgtttatgtt ttgattggtg gtaataagcg ttacgtgggt caggcagcag ggcaatcaat
    16021 ttcacttcgt ttatctcaac actttctcaa ggaatataaa gcttgggttg aatcagtttt
    16081 attctttaca agagcagatg ggaaaatgtc aaaagcagat acagattatc ttgaaaaacg
    16141 acttatccaa gatttccaag aaaaatctga ctatgaaatg atgaattcaa ctgctggtaa
    16201 tacttcgtat attgataaac ttcaaaaagc aaaatcggat cagctatatg acacagtgtt
    16261 tgaaattata gatgaaattg caaatatcga tttgtttggt acttccgaag acagtgaaac
    16321 ccaaatttat gaatttagtt catctggaca atatgaaatt gagtatgatg ggaaaaagat
    16381 atcttcgaaa tctgctagag gtttattggt tgattttgtt tctcagctat ttgcggatgg
    16441 aaactacaac aatcaactgc aagaattaat tgtcgatgag acgccgtcgt tcgctataat
    16501 tttaggacgt aaaccatcca tgtataacgg aagaccaaat tcagccaggg tagctgatgg
    16561 agtatgggtt tatgcaaatt tcaacaaaaa gaatgtcaga atcaaaattg ataaactagc
    16621 tcgtcaattg gggattccgg ttaaaataaa gtggaattaa tatttgttat ctcgccttta
    16681 gtaaatctat gctgaaggct tttattttag aaaaatagtt tgcgttcgcg aaaatattt
//
LOCUS       DAYYME010000058        16716 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000058 DAYYME010000000
VERSION     DAYYME010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.058"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(253..1464)
                     /locus_tag="ACHBFW_001542"
     CDS             complement(253..1464)
                     /locus_tag="ACHBFW_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9317850.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKASSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLKHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFENLLKKDIK"
     gene            complement(1583..2218)
                     /locus_tag="ACHBFW_001543"
     CDS             complement(1583..2218)
                     /locus_tag="ACHBFW_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFM9317851.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAKNNVLQAQTGYFGTETVSIALHN"
     gene            complement(2354..3007)
                     /locus_tag="ACHBFW_001544"
     CDS             complement(2354..3007)
                     /locus_tag="ACHBFW_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317852.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQVTAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFFKYGIQ"
     gene            complement(3110..3316)
                     /locus_tag="ACHBFW_001545"
     CDS             complement(3110..3316)
                     /locus_tag="ACHBFW_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFM9317853.1"
                     /translation="MPSYVVLQVVLKEKFIGKGSQNLSELENTINRQCSKGYRLHTIS
                     TTNGGSKGFGGGDRIQATLVFESL"
     gene            complement(3392..4138)
                     /locus_tag="ACHBFW_001546"
     CDS             complement(3392..4138)
                     /locus_tag="ACHBFW_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004564412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFM9317854.1"
                     /translation="MKQTTQQRLNQLMSERNLKQVDILNMSLPLQKETGIKMSKSHLS
                     QYVNGKSSPDQHKLYLLAKTLNVSEAWLLGYDVPKEDKKNNIPSIETIYNQLEKPRQT
                     KVYNFAEQQLEEQNRGNVVHFPKKEKLPTIKNSASAANPTELVYGDTVVEEEEFERVP
                     NNADFAVPIIGDSMEPVIRNGQFVFVKEQPDVEDGEIAIVEIDGDGVTCKEVYKDYES
                     QTIILRSINELYEDRVVSPEQIRIIGKVVF"
     gene            4298..4522
                     /locus_tag="ACHBFW_001547"
     CDS             4298..4522
                     /locus_tag="ACHBFW_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HFM9317855.1"
                     /translation="MSFDYSKLSGRIVEKYGTQYNFSIALGLSERSLSLKLNNRVPWK
                     STELQKAIELLDIPKEEIGEYFFNLEVQNV"
     gene            4546..5307
                     /locus_tag="ACHBFW_001548"
     CDS             4546..5307
                     /locus_tag="ACHBFW_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFM9317856.1"
                     /translation="MNTQKFENNLFELEVKTENGESLFNAETVARSLGFVETKNGKEY
                     VRWRTVNGYLKKYLSQEVAKNDFISEPMVYKLAFKANNVLAEKFQDWLATEVLPQIRK
                     HGMYATDELLNNPDLLIEVATKLKEERTLRLIAEQRVNELQPKADYYDRILNNKGLVT
                     VSTIAKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNR
                     QVKTRMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            5319..5576
                     /locus_tag="ACHBFW_001549"
     CDS             5319..5576
                     /locus_tag="ACHBFW_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317857.1"
                     /translation="MNEEMSQTIKVQKMIDDLTHAIDSQADKIIKELQGQKVWDAKML
                     FKTIKFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            5668..5919
                     /locus_tag="ACHBFW_001550"
     CDS             5668..5919
                     /locus_tag="ACHBFW_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HFM9317858.1"
                     /translation="MEQLALVNLSDLKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWVNSTEH"
     gene            5895..6068
                     /locus_tag="ACHBFW_001551"
     CDS             5895..6068
                     /locus_tag="ACHBFW_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317859.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPTEGAD"
     gene            complement(6182..6589)
                     /locus_tag="ACHBFW_001552"
     CDS             complement(6182..6589)
                     /locus_tag="ACHBFW_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317860.1"
                     /translation="MSFDNTITISIILALVALISPSITAVINNKHVESMKDKEIELQK
                     HDSKTQTIQTTFSTFLNNVGICIGSNTDKNISAVKASGYAVLPYIQNEDIEVMKIFLS
                     RFGYGNTDAEQKSLETYLIDKVLPILNKSLEKL"
     gene            6750..6974
                     /locus_tag="ACHBFW_001553"
     CDS             6750..6974
                     /locus_tag="ACHBFW_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317861.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            6984..7178
                     /locus_tag="ACHBFW_001554"
     CDS             6984..7178
                     /locus_tag="ACHBFW_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317862.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDAILEYVQQHYPKIELGGI"
     gene            7181..8203
                     /locus_tag="ACHBFW_001555"
     CDS             7181..8203
                     /locus_tag="ACHBFW_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFM9317863.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKDSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDITPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            8166..8981
                     /locus_tag="ACHBFW_001556"
     CDS             8166..8981
                     /locus_tag="ACHBFW_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFM9317864.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKMFMPIIAAVSKQTPSEAKLITI
                     DQDKMSYELIMLKENIERIVKVKNGEERPIPCGTCEYCRGNLSITGFTSMDDL"
     gene            8999..9781
                     /locus_tag="ACHBFW_001557"
     CDS             8999..9781
                     /locus_tag="ACHBFW_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317865.1"
                     /translation="MNLGYIKLYRKVTSSFVWTNSDMFKLWILCLMKASHEDRKFLFN
                     GQEVRLTSGQFVTGAHAIAKEYNEGVPSDKAIAWRTLWRWLKKFENEELLTIQSNARY
                     SVITIINWSDYQSSDKPVTSDGQSSDKPVTTNKNDKNDKNDKNNNPRNSRKTREYADD
                     DPNKKLAILLLKLIRKNRSLKEPDLNKWANTIRLTIEADKRTGKEVQDMIVWATNHDF
                     WSTVISSPTSLRKNFDTMVGQKNKRKQQNISNDELPETGEDW"
     gene            9781..10629
                     /locus_tag="ACHBFW_001558"
     CDS             9781..10629
                     /locus_tag="ACHBFW_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9317866.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVNGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKSDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            10626..10868
                     /locus_tag="ACHBFW_001559"
     CDS             10626..10868
                     /locus_tag="ACHBFW_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317867.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            11011..11379
                     /locus_tag="ACHBFW_001560"
     CDS             11011..11379
                     /locus_tag="ACHBFW_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFM9317868.1"
                     /translation="MATRSKYGNKKHEVDGITFDSITEAEYYDLALWQAQANGWKVKL
                     QERFELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQ
                     YQVPLILAKKYRNTFKEERF"
     gene            11394..11555
                     /locus_tag="ACHBFW_001561"
     CDS             11394..11555
                     /locus_tag="ACHBFW_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM9317869.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            11552..11878
                     /locus_tag="ACHBFW_001562"
     CDS             11552..11878
                     /locus_tag="ACHBFW_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317870.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            11875..12690
                     /locus_tag="ACHBFW_001563"
     CDS             11875..12690
                     /locus_tag="ACHBFW_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFM9317871.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            12692..12817
                     /locus_tag="ACHBFW_001564"
     CDS             12692..12817
                     /locus_tag="ACHBFW_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317872.1"
                     /translation="MGFFMAKILLFLGLVGAAYLVYALLSQTDDKEDDNNDEMKF"
     gene            12829..13140
                     /locus_tag="ACHBFW_001565"
     CDS             12829..13140
                     /locus_tag="ACHBFW_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM9317873.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEGS
                     K"
     gene            13137..13382
                     /locus_tag="ACHBFW_001566"
     CDS             13137..13382
                     /locus_tag="ACHBFW_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317874.1"
                     /translation="MIKKLIQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            13379..13795
                     /locus_tag="ACHBFW_001567"
     CDS             13379..13795
                     /locus_tag="ACHBFW_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFM9317875.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIYENSELLEVE"
     gene            13792..14130
                     /locus_tag="ACHBFW_001568"
     CDS             13792..14130
                     /locus_tag="ACHBFW_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317876.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMYGNSESAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            14127..14357
                     /locus_tag="ACHBFW_001569"
     CDS             14127..14357
                     /locus_tag="ACHBFW_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317877.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            14364..14567
                     /locus_tag="ACHBFW_001570"
     CDS             14364..14567
                     /locus_tag="ACHBFW_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFM9317878.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            14564..14860
                     /locus_tag="ACHBFW_001571"
     CDS             14564..14860
                     /locus_tag="ACHBFW_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317879.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMELINDK"
     gene            14937..15350
                     /locus_tag="ACHBFW_001572"
     CDS             14937..15350
                     /locus_tag="ACHBFW_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM9317880.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            15363..15749
                     /locus_tag="ACHBFW_001573"
     CDS             15363..15749
                     /locus_tag="ACHBFW_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317881.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            16300..16527
                     /locus_tag="ACHBFW_001574"
     CDS             16300..16527
                     /locus_tag="ACHBFW_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317882.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMLREAT
                     PKEIDQYKLVLIGIGWFKDEHIQTNMKRWLR"
     gene            16524..>16716
                     /locus_tag="ACHBFW_001575"
     CDS             16524..>16716
                     /locus_tag="ACHBFW_001575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317883.1"
                     /translation="MKLYYVEKANCWVVEGCLSKYATDIISTQFSELGLKVIFCGEFV
                     SVKEAVLPMTAKSRRSRGSS"
BASE COUNT         5666 a         2726 c         3485 g         4839 t
ORIGIN      
        1 aataaaaaaa gaacagcgta aatgttttcg aaatgctttc gcaccaacat ttacctgttc
       61 cgtgggtttg gcacccaatg ggccgtgagg ggctcgaacc cgcgacccgc tgattaagag
      121 ttcgttattt aatggtattt taaatgaaga taagcgaatt tacagcattt ttaaatgctt
      181 atataccgca aattatccca agtttttgta tatcctaccc gagtttatac ccgaaaatat
      241 aattttttag tgttatttta tatctttttt taataggttt tcaaacgctg tagttgctgt
      301 atctttttct ttgcgtaaga catggccata aacattcatt gtagtattaa aatctcggtg
      361 tcccaatcgt ttttgaagct cttttggagt catattagga tcgctaagca aataggtagc
      421 tgatgtatgt ctaatgtcgt gaaaacgaat gtgacgcaaa ttgtgacgtt tggtgaatcg
      481 tttgaacttc ttatacagat aacttccacg tggcaaagtt ccatcttgtt ttgtatcaaa
      541 tataaaatag tgttttaatt tctttatatt taatttccaa cgaatctttt tattctcttt
      601 gattaattca tctaacatat caaatagata agcaggaccg gttactattt tttcatcatc
      661 atcatttttc aaacctggca gcaaccgaat atctgatttt ccatccactt ctgaaatccg
      721 ctgatgaaat cttatttctt gtttgtcaaa gtcaatatct ttttcttcta aagctgcaat
      781 ttctccttgt cttgctcctg taataaatgc tagaacaatt aaagttttaa tttctatgct
      841 ttcctcaaat gctgctttta gcattgcatg aatttcttct tccgaataag gttcttcgat
      901 attctttttt acgccttttt cttttggtat ttttacattt tccacaggat tttctttaat
      961 tgctccgtac tcatttttag ccatcaaaaa taagttgtta acggcgtata acatacgaag
     1021 ctttgtattt cttgataaag gtttatcaga gtttctccca ggatcttttg tattgattct
     1081 ttgagcatta acaacaattt tctttatcat gtatggtttg atatcgatta gagggataaa
     1141 atcaaattta tcaagaaatc tattctcgat cattcgctta tagttatgat aagactttgg
     1201 ctctaaattt ttttcggcct ctgaaagcca tatttttcta taaaattcac caaaagttat
     1261 attgaacaaa tccaacgaat catcggtcat tccttcgaat tgctctaacc agatgttcaa
     1321 ttctttatat gccgctcttt tactacttgc ttttatagtt ttgctttttc tttttgccga
     1381 gccatcaggt ttgtatccta atacagctct tagtctatat gtttcgttgt ctacttgttc
     1441 taagtatcct gttacttctg acataattga atcagctctc tttctctgat acaataggta
     1501 ctataaagaa gcctatcgta taggtttgtt tctttcttag aacacgctcg ctttggtcgg
     1561 tggggcgtgt tttttatact aattagttgt gaagagcaat tgaaactgtc tctgttccaa
     1621 agtaacctgt ttgtgcttgc aatacattat tttttgcatt tgcttgggct tcagagatat
     1681 caaaaactat tttaccagtt tgttgcatat caggatttaa gctttccata aagaaagagt
     1741 tagtgatatt accattttca tcttgatttg ccgacataga tgcagccgaa tcagcttcga
     1801 aagtttttcc atctgctttt aatttgaaga atgagctatc aacagtcaca gccttatcac
     1861 ctgcattttt tacagataag tcaactacta agaaagtgtc ttttgcgtta gtaggaagta
     1921 cagatgggcc aacttgtttt actacttcaa cggagtttac tttgtattcc attttcccta
     1981 cagcaacgtc atcaccgatt ttataagtct tttcttcttt tgttgtagtt tcttttgtag
     2041 tttctttatt agtacttgtt gatgttgcag tagctgaatc tttgttttca ccgccactta
     2101 gagcaccacc aataccaaaa aacaaaagta ctattaatac ccaaaaccat acccgtttat
     2161 aaaacggttt tttaacctta tacattttcc catcttgacc cataactttt tttgccattt
     2221 aaatattcct catttcttgt tataatatat ttgtgatctc agaaatgagg tatgagtccg
     2281 tgttgcagca cgggcttttt tatttataac ttttttagag attataggca aaatagtagg
     2341 gcataaaaat atattattga attccgtatt taaaaaatcc taattgaact tcaccggaag
     2401 tcttttgctg tgttgtacgc aatgcttctt cttcagacat tccattctgt actttccatg
     2461 caacaggcga catcccgtat ttgttaacaa aatcagtaag tgataaagtg tcagcgtctt
     2521 gctgagcgct tgtttgttgg tcttctggat tttgttgaga tgctgcttgt tgttctttct
     2581 gatcttgact gataatattg ccagcatcat ctgtagtcaa tccattttca taaagggcca
     2641 cgccgaaagc ttcccactct ttgttggacc aatttgcacg atcagctgga gttgactgta
     2701 aagtgcgttg tttcatctgt tcatatgttt cttcttgagg tgcggtttgg attgtactct
     2761 gactggagct tataactgtt gggctaggtt ccgccgtaac ttggttggag cttgtaactg
     2821 ttgaactaga atctgtttta gatgtagatt tgctagtaga tgaactggtt tcagttgttt
     2881 cttttgtttt acttattttt gtttcttggt tagaagtggc atctgttgat tcagcttttt
     2941 tattatttga acaagctgaa agtagcagag cagtacttaa caacaacata acgctaactt
     3001 ttttcatttt atactcctct tttctttgat acaataagtt gtaaagaagc ctactatagg
     3061 tttaacatcc cacgtccaca aactttggtc ggtgggcgtg tttttttgtt tatagacttt
     3121 cgaaaactaa agtagcttgg attctatcgc caccaccgaa acctttacta ccaccgtttg
     3181 tagtggaaat agtgtgtaat cgataacctt tagaacactg tctatttata gtgttttcaa
     3241 gttctgatag gttttgcgac ccttttccta taaacttttc ttttaataca acttgcaata
     3301 caacgtaact tggcatattc ttttccttct ttctactatg ataataaata ctaaccccta
     3361 gccggagcgc caactacgac tggggtattt tttagaatac gactttcccg attattctaa
     3421 tctgttccgg tgagacgact ctatcttcat ataattcgtt tatagagcgt agaataatcg
     3481 tttgactctc ataatcctta tatacttctt tacacgtcac accgtcacca tctatttcaa
     3541 cgatagcaat ctcaccgtct tccacgtctg gctgttcctt aacaaataca aattgtccat
     3601 tcctgataac tggttccatt gaatcgccta taataggaac agcaaaatca gcattgtttg
     3661 gaactctttc aaattcttct tcctctacaa ctgtatcgcc ataaactaat tcagtcgggt
     3721 tggcggcaga agctgaattt ttgattgtcg gaagtttttc ctttttagga aagtgcacca
     3781 cgtttcctcg attttgctct tcgagttgct gttcagcaaa gttgtagact tttgtttgtc
     3841 ttggtttttc taattgattg tagattgttt caatggatgg aatgttattt ttcttatctt
     3901 ctttaggaac atcgtaacca agcaaccatg cctcactcac atttaaagtt ttagccaaca
     3961 aataaagctt gtgttggtcg ggcgatgact taccattgac atattgcgat agatgacttt
     4021 ttgacatttt tattccagtt tctttttgca aaggtaaaga catattcaaa atatcaactt
     4081 gctttaaatt tctttcagac attaactgat taagtcgttg ctgagtcgtt tgcttcatct
     4141 tatacctcct ttctgatact tattatatac cttattgaac ataagttcaa taaaaaagtt
     4201 aaattaattt gaacttttat gttgacaatg ttttatgcag gagttatact ttggttacaa
     4261 agttcaaaac atttgaacta gaaaggagcg aactaatatg agttttgatt attctaagtt
     4321 gtcgggaaga attgttgaga aatatggaac gcaatacaat ttttcaatcg ctttaggttt
     4381 gtcggaaaga agtttgtcat taaagttgaa taaccgagta ccatggaaaa gcaccgaact
     4441 tcaaaaagcc attgaattgc tagatattcc aaaagaagaa attggcgaat atttttttaa
     4501 tttagaagtt caaaatgtct gaacttaaga aaggaattcg aaaaaatgaa cacacaaaaa
     4561 tttgaaaaca atctatttga attagaagtt aagacggaaa atggtgaatc gttatttaat
     4621 gctgaaacag tagcaagaag tttgggtttc gtggaaacaa aaaacggtaa agaatatgtt
     4681 cggtggagaa ctgtcaatgg atatttaaaa aaataccttt cgcaagaagt tgcgaaaaac
     4741 gactttatca gtgagccaat ggtttacaaa ttagccttta aagcaaacaa cgtacttgct
     4801 gaaaaattcc aagactggtt agcaacggaa gtcctacctc aaatccgaaa acacggcatg
     4861 tacgcaacag atgaactact taacaatcca gacttgctga ttgaagtagc aaccaaactc
     4921 aaagaagaac gcacgttacg attgatagct gaacaacgag taaatgaatt acaaccgaag
     4981 gcagactatt acgaccgaat cttgaacaac aaaggattag ttacagttag tacgattgct
     5041 aagaactacg gtatgagcgc tgtatcgttt aataaattgc tacatgaatt aggtatccag
     5101 ttcaatcaaa gtggcacatg gctgctatac agtaagttcc aagacaaagg atacacgcat
     5161 attgagccat ttgattatga agataaaaac ggcaatcggc aagtaaaaac aaggatgaaa
     5221 tggacgcaaa aaggacacat cttcttatat gaaacattga agaagaataa ttacttaccg
     5281 atgattgaac gtgaacaaac agcataggag gtaacactat gaacgaggaa atgagccaaa
     5341 caatcaaggt acaaaaaatg atagatgatc taacacatgc aattgatagc caagccgata
     5401 aaatcatcaa ggaattgcaa ggacaaaaag tttgggatgc aaaaatgctt ttcaagacta
     5461 tcaaatttga aatgaatcca acaaaaagaa agctcgccga tgtgttggaa gaaaagttgg
     5521 cttccgctat caacgagcaa gaattactat ttgaaaccga tacttttaac agctgatgtt
     5581 ttatagatga cagtttcatc cgaatctata tcattataga ttcgattatt gcaatgtacg
     5641 aattataaat acaggaggaa ataaccaatg gagcaactag ctttagttaa cctctcagac
     5701 cttaaagctt ctttggcaga atctgagatt gcaaatgaag tgtgggatac aaagcaagct
     5761 gcggaatatt taaaaaccac aacacgcacg ctgactaaag atgccgaatc tggaaagatt
     5821 ccagcagcga aagttggcag agagtggagg tttagtagta tcgctttgta tcagtatcta
     5881 aaaggaggaa aaacatgggt aaattcaaca gagcactagt gttcagcgca cccctaatcg
     5941 tctatgcttt aggactttgg ggaagcagac aagcattaat cggcacgatc gtttacatgg
     6001 tttggatttt tatggggctt gatgaagctg agtacagagc gaaaaagccc accgaggggg
     6061 ctgactaaag tgtggttctg tttattaggt gtttatctcg tggccgtttt agtaaataac
     6121 tacatgagaa aacgtggcga atactggtat acatcctatg cggttctagt atgtctaatg
     6181 cttacaattt ttctaatgat ttattcaaaa taggtaatac tttgtcaatc aaataagttt
     6241 caagagattt ttgttctgca tcagtgttgc cgtaaccaaa acggcttaag aaaattttca
     6301 taacctctat atcttcattt tgaatatatg gcaggactgc ataacctgat gcttttacag
     6361 cagatatatt tttatcagtg ttactaccaa tacaaatacc cacattgttg agaaatgttg
     6421 agaaagttgt ttgtattgtt tgggtttttg aatcgtgttt ttgtaattca atttctttat
     6481 ccttcatcga ttcaacatgc ttattattta taaccgctgt aatcgaaggg gatataagag
     6541 ctactaaagc taggataatc gaaattgtga tcgtgttatc gaaactcatt ttttcaccat
     6601 ccagttttta aaccattata tcaaaaagga gagaagaaat aatgcaagaa ttagtaattt
     6661 tgaaaaataa aaaaagacac gaacgccagc aagcatgatc gtgtccaaaa gaaaaatctt
     6721 ttaaaccatt ataacacagg aggaaataaa tgatctcagt taaagggtta ggcgatgaaa
     6781 tattcgaagc aatgatgagc aaagcacaac aggacatcaa agaaaaaatc atttctgctg
     6841 cagcgcaagg acaaactagc tgcactgttc gatcgagagg aacgacacca agttttcttt
     6901 tggcgctaga agatgaaggc attttgaata tcaaacgaag agatggcagc gtcagattgt
     6961 tttggcaatt ttaggaggcc gtgatgaaag attttgattc tttaggcgcc agacagcaac
     7021 caccaaatga agctagtcct gttggagttg attggcaaga aaatccgtta tatccaggtg
     7081 atacttgcta tctaacagaa gaaggctatg taccagtaga cgcaattcta gagtacgtcc
     7141 agcaacatta tccaaagatt gaattaggag gaatttagga atggcaaatg atttaacaca
     7201 aacaacacaa cgttcattag atgagcaggt aatcagtaat cttggacggt tacaagaaca
     7261 aggtttagaa atgccaccag gatatagtcc acaaaatgcg ttaaaaagtg cttttttcga
     7321 actcactaac aatacaggag gaaacttgtt gcagatggct gcaaacaatc aagaaatgaa
     7381 aacctccatt tctaatgcac ttttagacat ggttattcaa gggttatctc cagcaaaaaa
     7441 acaatgctat ttcattaaat acggaaacaa ggttcagcta atgcgttcat attttggaac
     7501 aatggcggtc cttgatcgtg taactggagg ggcagatatt acgcctgtag tcgttcgaca
     7561 aggtgatgag tttgaagttg caatggatgg accaaacatg gttgtcaaaa agcatgaaac
     7621 aaaattcgag aatttagaca atgaaatcat tgcagcctat gtagtcatca agttaacaaa
     7681 tggtaaagag accacaacag tcatgacgaa aaagcaaatc gatcaaagtt gggctaagtc
     7741 aaaaatgaaa gattctggac cgcaaaagga atttccagaa gaaatggcta aacggacagt
     7801 aatcaatcgg gcagctaaaa cattgatcaa cacaagcaac gacaacgatt tattagttca
     7861 agctgcaaaa gatacgttag aaaatgaatt tgacaatgat cggaaagata taacgccaca
     7921 aacagaaaaa gtggctactc tcgaacaaaa attcttttca aacaaaaaga ttactgaacc
     7981 aatacaaaaa gaaccagatc cgattgtgat tccagatgat atccaaaatg aagtaactcg
     8041 agttgctgat gtacctgccc atccagaaat agagcaagca catttgatcg aaaatgagga
     8101 tatcaatcca attcaagaag aattgataga tattccagac tttggacgcg aggaaggtgt
     8161 agaagatgtc tcagaatttg aagacgacga gtaccctttc tgatgagaat tattactcca
     8221 atgaagctga ctggcagtac atgtcaacat cacaatataa gtccttttta aaatgtgaag
     8281 ccgcagcctt agcaaagttg aaaggcgatt gggcaccgac gtcggatccc aaagccttac
     8341 tcgtaggaaa ctacgtacat tcttattttg aatcaaaaga agtacatgaa gcgttcaaag
     8401 aagaaaataa atccaaaatg ttttctagta ggaagccgta tggattgttg aaagatttcc
     8461 aaattgctga acaaatgatt gaacgactta agcaagaaga agcattcatg aacatttatc
     8521 aaggtgagaa agaaacgatc gtgactgggg aactatttgg aacgacatgg aaaggcaaga
     8581 ttgattgctt gaatgttgag gatgagtatt ttgttgatat caaaacaacc aaagacatgc
     8641 atgagcggaa atggaacgaa aactacggat caagagaaac gttcattgtc aattttggtt
     8701 acgtacttca aatggcaatc tatcaagagc ttcttttaca gcaatacgga aagatgttca
     8761 tgccaatcat cgctgcggta tcgaagcaaa cgcctagtga ggcgaagttg ataacgattg
     8821 atcaagataa gatgagctac gagctaatca tgctaaaaga aaatattgaa agaattgtca
     8881 aagtgaaaaa tggcgaagaa aggccgatac cttgcggtac gtgcgagtat tgtcgaggta
     8941 acctttcaat tactggattc actagcatgg acgatcttta aaaggaggtg ctttgcgaat
     9001 gaatctagga tacatcaagt tataccgtaa agtgactagt tcattcgttt ggaccaattc
     9061 cgatatgttc aagctatgga tattgtgttt aatgaaggct agtcatgaag atagaaaatt
     9121 tttatttaac ggtcaagaag tacgtttgac aagcggacaa ttcgtcacag gagcccatgc
     9181 aatagcaaaa gagtacaacg aaggagtccc gagtgacaaa gcaattgcat ggcgaacgct
     9241 atggagatgg cttaagaaat ttgaaaatga agaattattg acaatccaat cgaacgctag
     9301 atatagcgtt ataacaataa taaattggtc tgactatcaa tcgagtgaca agccagtgac
     9361 aagcgacgga caatcgagtg acaagccagt gacaacaaac aagaatgata agaatgataa
     9421 gaatgataag aataataatc ctcgcaactc tcgaaaaaca cgagagtatg cagatgacga
     9481 tccaaataaa aaattggcca ttcttttatt aaaactcatt cgaaagaatc gtagtctcaa
     9541 agaacccgat ttgaacaaat gggccaatac gattcgtttg acgattgaag ctgacaaacg
     9601 aactggaaaa gaagttcaag acatgattgt ttgggctact aaccatgatt tttggtccac
     9661 agtcatttca tcgccaacta gcttaagaaa aaatttcgat acaatggtgg gtcaaaaaaa
     9721 taaaagaaag caacaaaata tttctaatga tgagttacca gaaacaggtg aggattggta
     9781 atgaataaaa aactaagtgc aatggctgca ccttacggcg gattaaaaac agcagatcat
     9841 aattgcccga aatgtggtga tccattgtat atctggaaaa caaaaaataa agatggtact
     9901 gatcgatgcg gtcctacatg tatcaataag atttgtggtt atcgagaaat ggtaactaaa
     9961 aatcaaaaag aagctatcca aaaagcgaat gaagcaatga aaaaggacgc tatcaatcga
    10021 atgcttaaca gttcaatgat tacagatgat gccatatgga ccttcgattt tgatggatac
    10081 aaagtagttg atcaggaaac agcacaaata aaagcgatgg ctcaagaatg ggctaaaaaa
    10141 attgtaaatg gtagcacgat tcatgcagtg ataaccggta gaacaggagc cggcaaaacc
    10201 catctaggcg ctgcagtgat taaagaagtc atgagggcat ctaattataa aattgcttgt
    10261 tcatttatca gttatcgaga acttttagaa cagttgaaat ttgcaatgaa tgatccagaa
    10321 gcaagaaaag ccgtaacagg ttcgctgatg gctgagataa agaaatcaga tttcgtggta
    10381 atcgacgatt tgggtgcaga actaggaaga atggaagaaa acaaccaggc aacgccttat
    10441 gatgttgatg ttctcacatc gctcacagaa gctcgtctaa acaaagctac gatattcaca
    10501 accaatttat catcgaagca attaaaacac gcgtacggtg agcgagtgtt ctctcgtgta
    10561 atgaatggaa cgaaagggaa catagctgta ttcaaaacaa cgacagacaa aaggaggaat
    10621 ccagtttgac ctttgtagtg aaaaaaatgt gctacttaga tagccgaggg cgaggagaag
    10681 ctagtgtcga gtttgctaaa caccacacga ctaaagaaga ggctgacctt gttgcaagtg
    10741 tttgtggcgg cgaagtagtc gaagttatta aacccaaaag acgattttca aaacctaaga
    10801 caaagccagt gaaaaaagag tgttgttgtc cgaaaagtaa ccaagcatgg atgagaggtg
    10861 caaaatgact tgtttaaaat gcaacgatga aacagttatt tggtataaga cattgcttgg
    10921 atggtcaact tgtgaacctt gtccaatatg caatgaaaat ggacgacgtt cgaaagaacg
    10981 actcgaaaga ctaaagaagg agtatagcaa atggcaacaa gaagcaaata cggaaacaaa
    11041 aagcacgaag tagacggcat cacttttgat tctatcacgg aagcagagta ttacgatcta
    11101 gccttgtggc aagcacaggc gaatggttgg aaagtgaagc tccaggaaag gtttgagctg
    11161 atgccgaagt ttgaactaga gggcaagaag tatcgcaaga tcgagtatat tcccgacttc
    11221 acattttaca aaagcggcaa gctggtcaaa gtcgtagatg ttaaggggat gcagacaaaa
    11281 gactttaaga tcaaggcaaa gttgttctgt catcaatatc aagtgccgtt gatattagcc
    11341 aaaaaatatc ggaatacgtt caaggaagag cgtttttaac gaggtggtcc atcatgacaa
    11401 cagaagaagt gattcaaatg cgaattcgaa gccttcggcg tgagattgac gatctggaac
    11461 gaacaaaggc agtgatggtc aatgaaacgg ctagaaaggc aatcgatttg cacatagaga
    11521 atttaagaag ggaaatccgt agattggagg aatgagcgtg gataaggaag cggcttggcg
    11581 aaaattaatg ttgctgattc aagatgagaa ctggcaagaa gatgaagcag tggttgctga
    11641 agttcagcgt ctaggaaaga ttgctaacgg acgtatacga aaaaagccag acaaaagaaa
    11701 acagcgcaaa gggaaaatcg tcgttgtttt acacgaaggc aaaattttga tgcaaggaac
    11761 agctagtgag ctgtctgcag aaactggata tacgcgtggg actattcgaa cgtacgcttg
    11821 gcgaaatcac accgatcgaa aagggcacga atataagtat ttggaggaag aaaaatgaac
    11881 gaaaacaaat taatcaaatt gggtgtagca ggagcagtaa tcgtaggtat tggagttatc
    11941 ggaggattta agttcttcga aaaaatcgat aatggatatg tgggtgtgcg ctattcaatg
    12001 aacggcggta tcaaagatga agcactgacg caaggtgtga aatttgtagg gattgacaaa
    12061 gtgatccaat atccaattcg cttgcaaact atccaatcaa aaaatatttc agtatctaca
    12121 agcgacggca aaaagacaac gattgatatc aaatatgact acaaagttga ttcaactaaa
    12181 gcagcaaaaa tgtacaagga atttgggaat atcacttcgg aagatatcga aagtggatgg
    12241 ttaaaatcta agcttcaaaa ggtcgctcgt gaagtttatg cgaaatatag tctgcttgat
    12301 gtcctttcag gagattcctc taaagttgaa gctgaggtat taacgaactt tgctaaatca
    12361 gttgaatcta aagggtttga agtcgaagac gtaacacttg gtgttccaga tgtcgataaa
    12421 gaaacacaaa aatcaatcga tgcgatcatt cgagctggtc aagaaaatga aaaagcgaag
    12481 ctagacgcag aaactgcaaa aactcaagct gatagtgaag cttacaagaa aacaaaagct
    12541 gcagaagcag aggcagaatc taatcgcaaa gtcgccgaat cagtaacaga caatttgatt
    12601 cgttatgaag aagctcaagc tcgcaaaaag catggatggg taacagtaaa tggagcagat
    12661 actgtagtta cggatgaagc aggcaaataa tatgggattc tttatggcta aaattctctt
    12721 gttcttaggt ttagtaggag cagcatatct cgtgtatgcg ctcctttccc aaactgatga
    12781 caaagaagat gacaacaacg atgaaatgaa attttaggag gagaaataat ggacgaacta
    12841 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcaga tccaaaagca
    12901 cagtttttga aagtagctga ggaattcggg gaaatagctt cggcgatggc aagaagtaat
    12961 gatgaactat ttaaagatag cgtaggagac gttatcgtca cgctgattat cctttccatg
    13021 caaaaaggga caaacgtaca agagtgttta gaaatggcat acaacgaaat caaaggacgc
    13081 acagggaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggaggg cagcaaatga
    13141 taaaaaagct cattcaattc agcatggatt tatatgatat cgaatcagga gcaacactat
    13201 ctgtggaatc ggaccatcta atcataaatt ttggtggaaa gcgccagatt attttgtggg
    13261 tagttgatga tgtactgttt ccagaaattg ttcatgattt cgaagaatca aaagcggttg
    13321 agtttgaaat agtgaaaaaa gtaatggaat tgattgaaaa atacgaggag gacagcgaat
    13381 gataccgaag tttagagcgt gggataaacg aaagaacgta atgagagatg tagccgtctt
    13441 gcattttact aaaaacggca aaacaaactt tattgaatat tggataaatc ctaccgaatt
    13501 gaaatcatat cacgtgcgaa acatcgacct catgcaatcc acaggaatga aagataagaa
    13561 tggtgtggat atatttgaag gggatatagt attagtcagc gtgcgaaatg gcttcgatta
    13621 cttagataat aaagtttgta ttgtcaaaaa ttcaatagat tattccggat tagtttgtgc
    13681 cactgttgat gaagacttag agtatcaaat ttttaacaca gagctgtttg aagaatacac
    13741 gtatgaagtc atcggaaata tatacgagaa tagcgagtta ttggaggtag agtgattatc
    13801 ttagctactg actacatcga ttctttaaaa gatgaaaatg ggaatgtccc agcattgcta
    13861 aattgtggaa ttaaaagcaa gaatagaatc attttgttac ggatatcggc tgatttagca
    13921 aaatatcttt atctgagcaa gacagcagta tattacactg cggctacacg ttatcagtac
    13981 aagggaaaaa caatcagtca ggattattat gatcgtttaa tcagtcctga tatgtatggg
    14041 aattcagaat cagcaattaa gctgtttggc gcaatagaga tatatgccga tgattttcca
    14101 aatttatggt tgaaggagga agcggaatga aatacgaaat accactaagt gaagcagacg
    14161 tccagtcaat tattaacggt cgggaggtta ataaaaaact tcctgatggt actgaattag
    14221 tcatcagaca aagttatttg aaagatatgg cagctccagt attaattgat cgttttaacg
    14281 tgactgattc tgtggtagag aaccacttga aagaatttcg atcaagtata aacgacactt
    14341 tcagattagg gagttgattg acaatgaaca ccagacaccg cagagtagca aaactaagaa
    14401 aacaggaact gaatgtacta aagacaaagt ttgaaaaaga atatggaatt tcagcagaag
    14461 aaacatataa agtggcaagt cagtgtgttg ctgatgcaag tgatgctatt cgtaagtttg
    14521 gaatttcgat attaagtgat gatcgtaaat gggaggaaaa agaatgaaac taaaagacgg
    14581 attttacgct agcagtcacg gtatcggcgg tttaatgcta gatatgccga caaagaatcc
    14641 taaaacacgt aagaaaccaa aagtcaaagt cggtgacatg gtccgctgtg aagcagagga
    14701 gttcatttat ccatttcgtg gatatgtaga acacgtctat aatcactcag cgatcattcg
    14761 cattgaaaac acgatggaat gtgataaatg gacagctaaa agcaaagaga atttagcagt
    14821 agtgagattg gtggatatgg aactaatcaa tgacaaataa aaaagccgga tcgctccgac
    14881 taacataata aaacagacaa gtttattata tcacataaaa ggagcagttt gaattgatgc
    14941 aattgttacg agaggtagat ttcaaacaga caagatgtaa tgcgagagat gtgctgaaga
    15001 actttcggcg tttggagcgg atggcaggtc gctctttgat agatattaag tcgccgatta
    15061 ttacggatat gccgaaggca ccgaagcacg gcaataaggc agaggatgca ttgattcaga
    15121 tgatggatat agaagcggag agagacgcga ttttagcggc tttgatggct cttagtctga
    15181 ttagtcgtca gatactctac tacagttttt gtgtgccaga cagcttctca aactacagaa
    15241 ttagccgtga agtgggttat tcagaaagaa gtatacaacg gatgaaatcg gaagctctaa
    15301 tagagtttgc agaagcatat aaacacggaa gaataattgc ttataaatag ggggaccata
    15361 taatgtggaa agattatgta tcgttgaaag agttgaaaaa agatcttgtt ttcaaaagaa
    15421 ttgtagaatg gtcagagagt gaattaattt tagaagatgg aaccaaaatg gaagttgtat
    15481 gtagcgaatc agattgctgc gcatgggccg aaggtgaatt caaaaatgta aagcttgatg
    15541 cagtgattac agatataaag atttttgata aaggtaaccg tctctataat ggtgacggac
    15601 atacctctta tgctgaagtc gttgtttatc ataatcgtaa tgagatcgct aaagcagaat
    15661 gcacagcgaa tgatgggaat ggcggctact attatagtgt ttgtgcttta aaggtcaaag
    15721 acaaactttg tatagtgact gacgcataaa aaaatggcgg ttttttggcg gaatgatggc
    15781 ggtttttagc catttatcag tgatattatg gtagtgtcga aagattagtg ataggtctga
    15841 gacaaaataa taataaaagg aacatcgttt tattattgtt tcacaattaa gcttcgatag
    15901 acagcaacgg aaatattaag aataaggatg tgaattttaa ctccttctaa attgttctta
    15961 ttatctatca tccgttgctg tttattgtta catacagatc gcttaggcgg tctttttatt
    16021 ttggccgaaa acctacattt tcgatggccg attattggag gaggaatagc atgttcaaac
    16081 tatctgaaat cattaagaag tccgacgttg agaaattaga gatgttaaaa aagaagttga
    16141 agaaatagca gtctcttcgt gaggctgttt tattttgctc acaaaaatag accactatcg
    16201 ggtaatagtg gtcaggaatt aaatgaaaaa gatgttaggg ttgttagcta agtataacat
    16261 cataacgctt acaaaacaat gcaagaagga taaattatta tgagaactta ctggtatgtg
    16321 tcattaaaca ataaatatcc gaagccaatg aaaggacagc ataggcgtgt agtcatgtct
    16381 gttcaaatga aggcgaagta ttcgattgta gaaatgctaa gagaagctac accaaaagag
    16441 attgatcagt ataagttggt tcttattggc attggttggt ttaaggatga acatattcaa
    16501 acaaacatga agaggtggtt gagatgaagt tatattatgt tgaaaaagca aattgttggg
    16561 tggttgaagg ctgtctgtca aagtacgcta cagatattat atctactcaa ttttcagagt
    16621 tgggattgaa ggttatcttt tgtggtgagt ttgtcagtgt gaaagaagcg gtattgccaa
    16681 tgacagcaaa gagcagacgc tcgcgtggtt caagtt
//
LOCUS       DAYYME010000059        16321 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000059 DAYYME010000000
VERSION     DAYYME010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.059"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..499)
                     /locus_tag="ACHBFW_001576"
     CDS             complement(101..499)
                     /locus_tag="ACHBFW_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFM9317884.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(496..1269)
                     /locus_tag="ACHBFW_001577"
     CDS             complement(496..1269)
                     /locus_tag="ACHBFW_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM9317885.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1266..1949)
                     /locus_tag="ACHBFW_001578"
     CDS             complement(1266..1949)
                     /locus_tag="ACHBFW_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM9317886.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2116..3003)
                     /locus_tag="ACHBFW_001579"
     CDS             complement(2116..3003)
                     /locus_tag="ACHBFW_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9317887.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3000..3869)
                     /locus_tag="ACHBFW_001580"
     CDS             complement(3000..3869)
                     /locus_tag="ACHBFW_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9317888.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4006..5292)
                     /locus_tag="ACHBFW_001581"
     CDS             complement(4006..5292)
                     /locus_tag="ACHBFW_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9317889.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDEE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5565..6143
                     /locus_tag="ACHBFW_001582"
     CDS             5565..6143
                     /locus_tag="ACHBFW_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9317890.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6143..7072
                     /locus_tag="ACHBFW_001583"
     CDS             6143..7072
                     /locus_tag="ACHBFW_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9317891.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7069..8805
                     /locus_tag="ACHBFW_001584"
     CDS             7069..8805
                     /locus_tag="ACHBFW_001584"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9317892.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8868..9704)
                     /gene="pheA"
                     /locus_tag="ACHBFW_001585"
     CDS             complement(8868..9704)
                     /gene="pheA"
                     /locus_tag="ACHBFW_001585"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFM9317893.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9701..10210)
                     /locus_tag="ACHBFW_001586"
     CDS             complement(9701..10210)
                     /locus_tag="ACHBFW_001586"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFM9317894.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10203..11498)
                     /gene="aroA"
                     /locus_tag="ACHBFW_001587"
     CDS             complement(10203..11498)
                     /gene="aroA"
                     /locus_tag="ACHBFW_001587"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM9317895.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11511..12602)
                     /locus_tag="ACHBFW_001588"
     CDS             complement(11511..12602)
                     /locus_tag="ACHBFW_001588"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFM9317896.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12619..13785)
                     /gene="aroC"
                     /locus_tag="ACHBFW_001589"
     CDS             complement(12619..13785)
                     /gene="aroC"
                     /locus_tag="ACHBFW_001589"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFM9317897.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13787..14851)
                     /gene="aroB"
                     /locus_tag="ACHBFW_001590"
     CDS             complement(13787..14851)
                     /gene="aroB"
                     /locus_tag="ACHBFW_001590"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFM9317898.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14884..15906)
                     /gene="aroF"
                     /locus_tag="ACHBFW_001591"
     CDS             complement(14884..15906)
                     /gene="aroF"
                     /locus_tag="ACHBFW_001591"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFM9317899.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4703 a         3616 c         2900 g         5102 t
ORIGIN      
        1 tactatttca taagtattca cgagaaaaat acaaacaaaa aagcggaaat aacgctcatc
       61 cgccttcttg tttgtgttta tttcctcttc aaacgcttgt ctatgattca gacttttctc
      121 tatcgttatt ttcatctaaa agttcttgca ttccgataat ttctcgatat tgatgaatct
      181 tagtgatacc aatgactttg tctacatcaa tactaaaaca aataccatta cctggaatat
      241 cgatgttgat gccttgacca atcgcatcca ttactttatt cgtcaaacta tcgggtacta
      301 atgtcaacac aatatctttt tctggcgaaa tgtccaaacc taaaaatttc cctgaatcat
      361 gtacaccaga acctcttcca tgtaagatcg ttccgcctgc tgcacctgct tcttttgaaa
      421 agtcgataac atcttcgccc attcctccgt caacgatcgt cacaatcatg tgaagattca
      481 atgctacaag atctttcatt tgctttcccc caatttttgc aatctttctt tttcgattct
      541 gtctttttca tcttttcgtt tataaatata tcctagtact aaagtggata atattggtgt
      601 caatgcaatc attgcaacca caccaaaccc gtcaatgatc ggatctcttc cttcaatttg
      661 tgcagcgatt gccacgctca ttgataaaat aaatgtcgaa cacatcgggc ccgttgctac
      721 tccaccgtta tcaaaagcca ttgctaaaaa gatcttgtcc acttttcgtc ctaaaataaa
      781 tgctagcccg tatcctggga tcaagaaata ataaagagag aatccgacga gcaaacgcca
      841 catagacaat cctactgctg cgccgatccc aattgaaatt accatcagca tgattttcga
      901 tcggattctc ccctcgctca attcttcaac ctgtttgatc attacatgaa tcgccggctc
      961 agcaaaccct actaagaacc ccattaaaaa gccaagggga accagtatat aattgtaagg
     1021 caatcccgca atagcactgc ctaaatgctg tcctaccggt acataagcca tattgactcc
     1081 gtgcaggaaa aggatcaacc caatagtcgt tatcaagaaa cctttcataa tgtcaaagaa
     1141 acgctttttg cgcatacgaa aactaatgat attcattaaa acaaatagga ttacgatagg
     1201 tgccactgcc aaaatgactt ccatggtaac gatttcgatt ccatctatca cttgctgcca
     1261 gaaactcatt ggaagatcac ccccaaaatc atcacggcaa gaatcggccc taacgaagca
     1321 ataccgacca ttccaaaact gtcattttca tttttctttt gtttgatcat actagtcatc
     1381 cctccggcaa gggccaagat aaatggaaca gttactggac cagtagtcac tccccccgaa
     1441 tcaaaagcaa tcggcataaa ttcggaactg gtgaaaaatg aggcgatcaa aacgcctatg
     1501 tagcctatag ccatcaattg ataataggat agcttgaata cgacccgaag cagcgcaaag
     1561 gctagaaata ctccagtgcc gacgctgacg atcccaatca gcaacactct gccaatctct
     1621 ccgtcagata tatcatatac ttgttgtccc aaaacttgca cggaaggttc agcaatcgtg
     1681 atcacgatcc cgatagcaaa tcccaaactg accaaaacgg tcaaactttt ctttttgatc
     1741 atatatttcc ctactagatc tccgacttcc atcatagaat aatctgctcc aaacaaaaag
     1801 agcgtcattc cgatcatcat gattgccgca ccaaccagaa atgaagcgag gtcttctcct
     1861 tctaacggtg caaaaacaaa cgttaaaatg acaatcagga cagtcatcgg caatatagca
     1921 gcaatcactt ctttaaaatt ttttagcatg tatcctcctc ctttactctt taagtttacc
     1981 ttaaaatgaa agagttttcg agcacaaact taaaacgtta tcatttaatt tttctcaaca
     2041 taaaataaca cacgtcccaa aagtatcttt ttacttggaa aaatgacttt gaaggaacgc
     2101 gcttattgtg tttttttatt ttacagagcc gatcattcct tgaacgaatt gcttttgcaa
     2161 aacaaaaaat acgatggcag ttggtaaagt agcgatgact aatccagcca tgatcattcc
     2221 atagtctggt gtataggaag cacccatagc agataaaaca agcggaaccg tccgtttatc
     2281 aggagattgc aaagctacta agggccataa ataattgttc cagctactca tgaatgtgat
     2341 gattgcagcc gcggcatagg tatttttagc cgtcggcata tacactctaa aaaaaatact
     2401 tagttctttt agaccgtcta atcgagcggc ttctactaga tcttttggaa aagatttaac
     2461 attttgtcga aagaagaaga tcaaaaaagc agtgctaaca gatggcagga tgacaaccaa
     2521 gaaactgttg attcccaaaa ccgttccgtt caattgtgaa aacattcgat ataaagggat
     2581 catcaatgcc gcaaaaggaa ccatcatcga tagcagtaga atattgaaaa ttaggtcttt
     2641 tcgtttactt tgataaatct caaatccata tcctgccatt gacgaaatca gcaatgcaaa
     2701 cacagtggtc accaaagcga tcatcgctga attccaaagg gaacgagtaa aattcagctc
     2761 attggaaaac aaattttgaa aattgatgat caagttactg ccaatcttta tttttcccga
     2821 tgtgatatcc acgggtgtat tcgtcattcc caaaaccatc cagacaaaag ggaaaatgga
     2881 aataaatgat aggatgctta atgctgtata ctttaatact gacccaattg tcgaacgaag
     2941 ttctaatgct tgttttttga cgttggggcg ttcgattgtt tctttttcta ccatattttt
     3001 catcgacttt ctcctcctac tttcgtttgg attatcgaca atatcacaat aaaggaaaca
     3061 ataacaaagg aaactgctgc cgcatatcca aaattaggcg tgaatttata actcaaatta
     3121 tagatgtatt gagatagggt ggtcgttgca tttgctggtc ccccgccagt aatattttgt
     3181 acttcatcaa aaagctgcaa tgtcccgatc gtcgaagtaa tggaagtaaa caaaatgatt
     3241 ggttttaaat ttgggatcgt cactttgata aactgctgcc atttagaagc accgtcaata
     3301 gatgctgctt cataaatctc cgaatcaata ttttgcatgc ctgaaaggaa aaaaatcata
     3361 ttgtatccag tccaacgcca agtgatcgaa agaatgatca gaactctcgc ccaaattgga
     3421 tgagtcaacc agccaatcgg tgtgtcaatc ataccaatgc ccaacaacaa gttgtttaca
     3481 aggccagatt gagaaaatag acttttcatg atcagagaat aactgaccat cgaagtgata
     3541 catggaagaa agatagcggt tcgaaacaac cctttatatt tcaacttttt atcatttaaa
     3601 aggttggaaa taaaaagtgc tagaaacaac atgatcggta cttgtacaag taaatacagg
     3661 aatgtattaa aaaaagcttt tttaaaagtt gcatctgtca acatccgctg ataattacct
     3721 aacccgttaa atgtcatatt gacaggggga ccactttgaa aagacgtgat caaagccaaa
     3781 accatcggaa taaaaaccat gattgatatc aaaatgactg gcaagattaa aaaagcatgc
     3841 ccgtttttga ataaatcttt ctgcttcatt cttttccctc caattctatc attgattatc
     3901 tacatgaaaa gacaagaaag acaatatctg taaggcagac gtgatctttt ttgtcttttc
     3961 gtttgtctga tgaaaaagcg aagaaattca cttttttacc accgatcaat tagccaattg
     4021 tgcttctact tgtttttgtg tatctgctag taccttgtct gtctcttctc cattgatgat
     4081 tcgatgaaga gcttcagcta cgactgcttc aattgcatag gtctcatgtc cgtaattcac
     4141 ttctggtatt tcagatgtcc atgttgaaaa atcgtcaaag atcttttgac cactgtaaaa
     4201 ttcagaagct tcttgataag cttcttgttc attcgcgcta agcatcgtag atactagtcc
     4261 gatttctttc gcaagagtgc ccatcagttc ttcattcgaa gcaaacgttg cttctaggaa
     4321 atctttcgct gaatcttctc cagctactcc tttgatcaca taccagccag cgccgccaag
     4381 gttagaagct tgcgcttttt gcagattttc tggaagtgca ggtattggtg caatcttcca
     4441 ttttccagat tggttttctg ctccttggat cgttgaagaa taccaagcgc cagttgggct
     4501 agaagctact gctcctgatt gtatcgcagt tactccagca ttccagtcag aggtttgttc
     4561 gactagatct tctttcaaaa gagtggcaaa caattcaaga ccatacttta aagatgcatt
     4621 gtcttggatc gtgactgtct cctcatcttc agatgtatac cattctcctg cctgttgcat
     4681 aatcatcctt actcgaccaa ggtcagaagg attgacttca gcgattttct ttcccgtctt
     4741 ttctttgaca tctcgcgcta cttgaatata ctcgtcccac ttcagattct ccatatcctc
     4801 ttctgtataa cctgcttctg ccatcagatc cgtacgatag aagtttgctg ttacgcccga
     4861 atcgaaagga acaccgtata ttttatctcc tactttgttt accgcaaatt tatacggtgc
     4921 aaaatgatct tcctttacaa tagcagacaa attagcgaat gcatctggat aagaagtcaa
     4981 atagccttgt atcctataat cttcaatcaa gacgatattg ggcaatcctt ctgtattccc
     5041 gctcgctaaa gcggtgttca gtttctgaac gatatcatcc tgagacatcg taacaacttc
     5101 tatctctgtt tcttcgttct cataaacttt ttttgcttca ttcactgctt tgatattgaa
     5161 tgtctcatcc cacgcccaga cagtgacttt ttcttctttg tctgctgtac tactagcact
     5221 tccgcatcct gttaagaaac ctgatgctgc cgctaaacaa acgactccag caattaactt
     5281 tctgatgttc atttttatcc gctccttttc catccattag ttgtttacgt ttgctacatt
     5341 catcatacca aaatttgaaa acggtttctt tattattcag atacttcaaa aatttctcct
     5401 aaaataataa aaaaatacaa atcaactatg acaaaaggaa taatcttagc atctagacca
     5461 aagatcgaaa tggtgaggtt tctttaaaat aaaaatatcc ttgcattttc tcctagaaat
     5521 aagtattttt aaattacaag actattctta aggcgggtaa aaaaatgcca atacgcgtaa
     5581 ttcttatcga tgatgaacag acgattttag atggaatgaa acagttgatc aattggtctg
     5641 agcacgggtt tcaattaata gggacttttt ccagtccgct gaaagcgctt gatttttctt
     5701 tggtacatgc aatcgatatc gtggtcacag atgtaatgat gcctgaatta aatggaatcg
     5761 aattatcgcg gctattaaaa cagcaacaac ctgaaacaca gatccttatc ctctctagct
     5821 atgatgaatt tgatatggtt aaagattctt ttaaagaagg cgtcgctgat tatcttttaa
     5881 agcctcgatt gagtcctgat ttttttctaa attccttaac tgccgtttca caaaaaatca
     5941 aaaaaaagcc caacaaaagc ccaatcacca atcgttatga gcagatctgc gagaatcttt
     6001 cacagcagat tgctggacaa actcgtccat tgcttgaccc tcttcttttt gaacacacct
     6061 tgtttttctg ttatataccg aaaaacggcc attcagttca aaacaaggct ctaaacaaaa
     6121 acagtcgatc acccattcat tgatggatag cgacgattcg tttactattt atcctgtttc
     6181 taccttttcg gaagaatccg gctatgtaat caatactgat tccaaagata cacttaggaa
     6241 gtttgttcaa aagtttcgtc aagaagtttt tccaggtgat ttttttgttg tttctgattc
     6301 ttttgcaatc caagatatca atgaaatatt ccaagagatc agaaattgca ccaaaggaca
     6361 acgtttctat cataagggaa agaaccttgc ttatcagcat gaacttcttc cgttattaac
     6421 caaccacgag cagcaagcaa gtagttattt aacaaaaatc gttcataaag actacgcttt
     6481 agctgtcaaa gaacttgaga atttcttcac gacgatattg gaacagccta ttgatccagc
     6541 ttatttgaaa catgaggcaa tcaatcgttt ctatgcatta ctcaacgctt tggctgacga
     6601 gcatgttggg aatgaagaac tttctttgct caagatcgcc cttccttctt tattagccga
     6661 tgctgagtat ttcgaagatt tttctattct tgtcaaagaa acacttgaga aactcaccca
     6721 cttttctgca aatgcaaaaa aagacaattt cgatatcttg actttgatct acgagttcgt
     6781 ccaggaaaat tatgagaaag aaatcaattt acaactttta tccgaaaaat atcatttttc
     6841 atatagctat ctctcagcca tctttactga aaaatttggt atcaactttt caaaatatct
     6901 aaaaaaagtc cgtatcagta aagccaagct ctttttgacg gaaacacggc ttactttgac
     6961 ggaaatctgt gagaaagtcg gctataccga actgggttat ttctccagag tttttaaaga
     7021 agagacagga atgacacctt ctcaataccg taaaggaact tttgtaaaat gagaaaaaaa
     7081 atcaaagagc atgaactctt tgccaaactt ctgttcattt tgttcatcgg tctttttctg
     7141 caagccatcg tcatttcttt gtttatttat catcgatcaa gggatgccta tatccagctg
     7201 ttcaatcaat cgaatgatgt ggtactaaaa aagattcaat ctgaatttga aagtttgaat
     7261 gatacaatcg agaatacatt agccgctttt gattctaatc cagctgtcaa aagctatttt
     7321 tccaatgatc cagcccaaca tatggaacag cttcagcaac taagaacaat ccaaaaaatg
     7381 aatgactctc tttcaaaaat ccatccaatg atcgattacg atgtattgat ctttggagaa
     7441 aatggaagaa cttttgtcgg aaacgacatg ctcactgccg tatctgccga ttcttttttt
     7501 caatctgcga ttgctcagag agtgaatgaa cgtgctgctg atacacaaat gctttttgca
     7561 caccacggat tgactcttcg cgataagaaa gcacccagcg tcttctttgt caaaaaactg
     7621 aaaaatacat tgaatcacgt ttacggatat gctgttttat cgatctcatc caagcaacta
     7681 gcgaatcttt ttcaatctgt agtaaatcct gagatatcga agatctcctt catgaacgaa
     7741 gaacagacga tcatcgcatc aaatgaagaa aatacaattg ggagccgatc aatgattttc
     7801 aaaaaactaa cagtaggtga aacaacgatt tcaaatggac agcagttgac ccgtcttcct
     7861 ctgtaccgcc aggattgtac attactcagt caagtcgatc ttcattcttt atctaaccaa
     7921 atgggcgtga tttttccgat tattttgtat aatatcgtga cacttgcttt tgctggctca
     7981 ttggtattta tgtatttgga tcgacacaca aaatcgatct accgcttgat tgattcgcta
     8041 aaacggctag aggaagatgg cttaaaagct actgttcctg tacaaggaag ccatgaagtc
     8101 caattgctag ggaatacgat caatcaattg ctaagtaatg ctcagcatca ttatgaacag
     8161 acgatccaag acgaaaagaa gaaacgttcg cttgaaatcc aaactatgca agcacagata
     8221 cagccccatt ttatttataa tacgctgact agcttgaaat ttttgatttg gcagaaaaaa
     8281 acagatgaag cagttcaagg aatcgacagt ttcattactt tactaagaag tacgattggg
     8341 aaaaaagaag aagtgattcc agtcaaggaa gaaatcaaaa gcgtacaaag ttacatcgat
     8401 cttttgagta tgcgatatgg cgaaagaatc tcggctaaaa taatgattcc tgatgaactg
     8461 cagtcattat ctatgccgaa tatgatcctt caaccaatta tcgaaaacgc ctatcttcat
     8521 gcttttcaca ataaaacgca aggattcatc acggtgtacg gcaaaacaaa aaatcagctt
     8581 cttttattcg aagtgattga taacggcgat ggcttcgata ctgaaaatac caaacaaaaa
     8641 aatgatcatt tttcaggaat cggcatcagc catgtagatg agcggatacg cctaatgtat
     8701 ggcgatgcat atggcttaac gatccaatca gtgattggga caggaacgac tgtaacgatt
     8761 tgtctaccgc ttcttcctaa aaatcaatca gaatatcaaa aataaagaat agccaagcgc
     8821 tgattttttt attttaagtt aaataaatca atcagtattc ttgactttca tctacgcgtc
     8881 tcttctactg tcagcactgg ataacagccc aatatttgac tttctcctcc gagcatcttg
     8941 atttcttgga gcgcattctc tactaatgtc attggacggt ccaacaacaa atcgatcacg
     9001 aaaaaatatt ctcccaagct agttttcaat ggtcgggatt caatcttact taagttgatt
     9061 tcccgccagc caaaagctgc cagcatttta tgaagcatcc ctggccgatt agcaggtagt
     9121 gtcaagatga cactcatttt tactggagct gactgctggc tggtcatctt tcggtcaccg
     9181 acgatccaaa aacgtgtctg gttcaactca ttatcttgga tattctcagc taagatctcc
     9241 aatccaacat gctgtgctgt ttctaatgaa gcaatcgctg ctgcgtcttc tttcggatgt
     9301 tctgctacat acattgctgc agcagttgtt gattctgttg ctactaaagg aacattagga
     9361 taatgcgttt ctaaaaactg ttgtgattgt gctagtgctt gtggatgtga aaggattttt
     9421 gtaatctttg ttgctggatt gcctaaaagc tgctgcttga taggaagaac gatttcactt
     9481 ttaacttcga cttctggatg tttggaaaga agatcgattg tgtgatgaac agctccttcc
     9541 aatgaattct ctacaggaac gacagccaga tcgacttgtt tgcgaaacaa agcgcgcaaa
     9601 caaacaggga tggaagcata cgcagtcaat tgttctgtag gaaatagctg acaagccgcc
     9661 tgataagtga atgaactttc tggccctaga tagctgacgt tcatatgctt ttgatccttt
     9721 ctataatggt atcgatgatc ttttctggtg tttgattggt cgtgttgatt cgatgtgtcg
     9781 cactttcttc ataccaagaa agtcgttgtg ccaataatgc ttttatttct tctcttgttt
     9841 ttccaagagc taaaggtcga atagacgttt catcttgttg gatacgatca actagacagt
     9901 ccgcttctgc ctctaagtaa agaacaatcg gctgtgtttt taaaaaacgc cgattttctg
     9961 cactttggac aatccctcca ccagtcgcga taattttgtc ttcatttgac agctttctta
    10021 aaatatctga ctccactttt cgaaagcttt tttcaccgta atagtgaaaa tattcggcaa
    10081 tcgacaattg gatatgttcc tcaatctttg tatctaaatc tacatactct ttttgtaaac
    10141 attgagctaa ccctttaccg attgttgttt ttccagcgcc catgaaacca atcagcaaaa
    10201 tgctagccat gagcttctcc tcctgacaca agttctgcta aatcgtcaaa aaatgccgga
    10261 taagagattg aaacagcttc agctccttct agttcgcaag gtccatcagc tatcaaagca
    10321 gctacttgca gcatcatacc gattcgatga tcgcctctgc tggacacttt tgcgccatgc
    10381 agaggtgtcg gcccatggat gatcagaccg tctgctgtcg cttttatctt tgctcccatt
    10441 ttctgtaatt cttctgccac cgcatcaatt ctgttggttt cttttacttt taattcttct
    10501 gcgtctttga tgatcgtgat tccttctgct tgagtagcca ctaaagctag aatgggcaat
    10561 tcatcaatca gacgcggaat gatttctcca ccaatcgttg ctttcttcaa atgactcgtc
    10621 ttgacactta aatcagccga ttggttctgt tcattgtgat tcgtctgggt aatcgctgcg
    10681 cccatctcct ccaacacatc taagatacca gttcttgttg gattgacccc gacatttttt
    10741 aacaacagtt ggctttccgg aacaagaagt cctgctgcta gaaagaaagc agctgatgaa
    10801 atatcacctg gaacagttat ctgttgacca gttaattttt gcggtccggt cactatgatc
    10861 gttttatctt ccactgtgat ccttccgcca aattgacgaa tcatttcttc cgtatggtta
    10921 cgggaacgtt ctttttcaac gattacggat gtgccttctg cttgtaatgc cgcaaacagc
    10981 aaagcagatt tgacttgcgc actggctaca ggcatattgt atcggatagg tgataaagag
    11041 cgggttcctg tcacagtgat tggtagatac tggtcatttt cttctccttg caagtcagcg
    11101 cccatctcgc gtaaaggttc catcactcgc cccattggac gtttagacaa cgaagcatcc
    11161 ccgaacagag tagtcgtaaa aggctgtcca gccaaaatac ccatcaataa acgtatggtc
    11221 gtaccggagt ttcccatgtc aattgattcg gttggttgga caaatgagtg gctaccgcga
    11281 ccatgaatct tgatcacctc ttcctcttct tcgatctcga cacctaattg ttggaaggca
    11341 tgtaaagtac tcagacaatc ttctgcgcgc aaaaaatttt gtatagtcgt cgttccttca
    11401 gcaattgctc caaacatgat gctgcgatgg gaaattgatt tatctgctgg tatcctaaca
    11461 gtccctctta atccatgtat ttgttgtaat aattgcattt atccagcctc ctatttgatc
    11521 cagcaatgat aactggtatt agacataatg attctttttg cttgtaacaa atcctgttgg
    11581 tttttaaaac tgatttgcag tattcctaaa atatcttccc tggtctcttg gatcttcaaa
    11641 ttaatcaaag aaactcccgc ttttccgatc agtccagtta cttctgcaat aacgccaggt
    11701 tcatctggca catcgataaa taaatcaaaa aaggcaggaa tcgcgccttg cttatggaca
    11761 ggcaactggt ctcgactttc ttttgcccga ttgaaaaaag aaaagatttc cgattggttc
    11821 tcagtcatga tccattctgc tatttgtgtc atttcttgtt gccaatctgc caatcgttcc
    11881 aataatatct gcttattgct caacaaaata tctgtccaca tgacaggatc tgaggaagcg
    11941 atcctagtga tatctcgaaa gcctcctgct gccagttgtt tggctcgcgg atactcttga
    12001 ttgaattgtt ctgcctgatt gactaagcca gctgccagaa tatgtggaaa atgacttagc
    12061 attcctgtta tacggtcatg ttctcttgct gtaagagtta catattttgc tttggttccg
    12121 caaaatagtt cttgcagttt ttcaacatct tttttcgtct tttccaaagg tgtaaagatg
    12181 taataggcat tttcaaatag tttagcatct gctgcctgta tacctgattt atgagaacca
    12241 gccattggat gtccaccgac aaaccgaaga tttttctgct cagcggatgt cacgacttgc
    12301 tgcttcgtac tgctgacatc cgtaatgatg acgtgatcag acaagttgat tctttcaagg
    12361 acatcaagat aagctaaggt tgtacggacg ggaacggcta ataagataac gtctgcttgt
    12421 tctgcacctg tttcaaaatc acttgcaacg taatcaacga tctgctgttc tactgcttgc
    12481 tcacgggttt ttgactgact atcccaacca actactatca gttctggatt ggtctgacga
    12541 atacaaagtg ccatagaagc accaatcagt ccaagcccca cgataaaaac atgttgtgcc
    12601 atcttatttg cccccttttt agtaatgttt caagtagtca cggtactcct tcactgcagc
    12661 tttcaactca acaagcgaat cacttgggaa ttttgacaat agctctgtcg ctaccacagt
    12721 ggctacaaca ttttccgcaa cgacgctagc tgcaggaact gctgtactat ccgaacgttc
    12781 tacacttgct ttgtaaggtt cttttgtatc gatatcaaca ctttgaagtg gcttgtacaa
    12841 tgtaggaatg ggtttcatga ccccgcgaat gacaagtggt tcgccattgg tcattccgcc
    12901 ttcgaagccg cctagattat tggtacgtcg gaaatagcct ctttcttctt cccataggat
    12961 ctcatccatc acttctgagc ctggtaatga accagaagtg aatccgaccc caaattctac
    13021 gcctttaaag gcattgatac taaccacagc ttgtgcgatt ttggcatcta gcttcgtgtc
    13081 ccattgtacg tagcttccta atccagctgg aacgcctcca acgagcactt ctacaattcc
    13141 tccgatcgta tctccatttt tctttgtctg atcgatcaaa tcttttattc tttgctcaac
    13201 tgctggatca acaactcgta cttctgacgc ttcagaaatt tctttgattt cttgaatcga
    13261 gatatttcca ggtatttctg cttggattcc accaagacaa atcacgtgtc ctgctacgat
    13321 gatgtcaagt tcttccagaa ttttcttggc aattgcgccg attgctacac gcatggtcgt
    13381 ctctcttgca gaggaccgtt caagcacatt ccgaagatct cgatgatggt atttcatacc
    13441 gcctaccaaa tccgcgtgtc ctggacgcgg acgtgcaact cgccgcagtg attggtcttt
    13501 ttcaggcact tctgctacag acatgacagt tgtccaattt ttccaatctt tattttctac
    13561 cactaaagtg acaggtgaac caattgtttt gccgtgacgg atgccagacg tgatccgaac
    13621 acgatctttt tcgatcttca tccttcctcc tctgccgtaa ccagcttgtc tgcgagcgag
    13681 ttcgtgattg atttcttctt ctgacagagg taatcctgct ggtagtcctt caataatggc
    13741 tgttaattct ggtccatggg attctcccgc agtcatataa cgcatcttac agtcctccta
    13801 agtaatcttt gaatgtctct gtgggaacag gaacgatttt tgcttgccca atcttttcta
    13861 gcaagatgat attcaattgg cttcctcttg ctttcttatc atgggttatc gcgtggaata
    13921 attcatctgg atgacttaga tcgtacgtaa tcggcagatg gaatttttga agtatccttt
    13981 ctaaagcagc tgtcgatcca gttggagaca agccgatttt ttctgcatga gaagtgatgg
    14041 ctaccattcc aatagctact ccttctccat gactgactac cccatatcct gctgtatttt
    14101 caattgcatg gccaattgta tgaccaaaat ttaaaataag tcgatttcct tggtcaaaga
    14161 catcttcttc cactactttt cgtttgattt ccaacgtagc agtaatgatt ggaacagcat
    14221 gttccagaag atctttttcg tctttcagct cgttcagctg tttccaaagc gttgcatctg
    14281 ctatagctgc tgatttgatg atttcagcga ttccttctcg taaacgacgt accggcaaag
    14341 tttttagaac ctcaggatca atcaatacac cagctggctg gctaaatgtt ccgacaaggt
    14401 ttttcgcttt gtttgtattg actgctgtct tcccgccaat actagaatct acttgtgcaa
    14461 gtagcgtagt cggaatttga acgaaatgga tcccacgcat gaacgtcgag gccacgaatg
    14521 ctgctagatc acccaccatt cctccaccaa gtgcaagtat cccatcgctg cgggtaaaat
    14581 cattatctgc taaaaaatcg tataactcag ctgcttgttc taaagattta ctggtctcac
    14641 ccgccggtac gattgaaagg actggttcaa aattagcttg tctcaattgg aacatgattt
    14701 tttctgcata tagtggagca acgtttgtat cagtgatcac agctactttt tgaggtctcc
    14761 aaatcgtttg aagccatgat ccagcctgtt ctagtgcgcc tttttcaata tgaatatcgt
    14821 atttttggtt atcaacatgt acagtaagca ttcgtttcac ttctttcttt taaaaagctg
    14881 gaattaatcg agtgtctgaa tcttttccat tgcttctttc atgatcgcaa cttctttcat
    14941 catgcgtgag taagtctttt cattcaacga ttggggaccg tctgaccaag catttgcagg
    15001 atcaggatgg atctctacga tcatgccatc tgctccgctt gccacacccg cacgagccat
    15061 tggtggtaca agttcccaaa ttcctgtacc atgacttgga tcaacaatca cagggaaatg
    15121 agttaatttt ttcatcaatg ggacagcact taaatcaaac gtattacgtg tcgctgtttc
    15181 gtaagtccga atcccacgtt cgataaaaat gacaggagat ttgtcttcga ctgcaatgta
    15241 ttctgcagca ttaagccatt cattgatcgt accggaaatt ccacgtttca atccaacagg
    15301 tttacctgtt ttaccgacag cagatagcag tttaaaattt tgcatattac gtgcgccaat
    15361 ctgtaggata tcgctgtact cagccaccat atcgatatgc ccttcatcca tcacttcagt
    15421 aatcactttc attccaaatt catcagccgc ttgacggatg tatttcaatc cttcttcttc
    15481 taaaccttgg aaagcatatg gagaagtacg tggtttgaag gcaccacctc gcagaatttt
    15541 tgcgcccccg gctttggcca ttcgcgcaca ttctctgatt tggtctaatc cttcgataga
    15601 acaaggacca gccatcgtca cgaaactgcc gtcaccaatt tttactccgt ctacatccac
    15661 gactgtattg ccaggatgga attctcgaga agtcaatttg tatgtgttta aaatacgcac
    15721 agcattttct actccttcat agcgtgtaaa tgctccttct tgtaaacttc gcgtatcacc
    15781 ttttagtcca atcactactc gttctgttcc ttgatcgatt gagactttga atccttcttt
    15841 ttcgatatga tcgacgactt tttttatttg ttcttccgtt gcttcttttt tcataattac
    15901 tatcattgtt tttcccgctt tcttttgttt acttatctat cttggctgat tttctctact
    15961 ttttcaaggt ggttttacat taattcctac taaatgaccg tgtcctgttg ttctcatttt
    16021 cgctccttct ttcctactta tgacccagat aatcaacaaa aaaagatgtc cctgtaggaa
    16081 attgcctcta cagtgaacat cttcaacgag ccttaaaaag cgctgcaaaa atataaaata
    16141 gccactatag atgaatttct ctacgtggct gtaagtcgtg tttaaaaatc tttagccatc
    16201 atagatacaa acctggtttg tttgtaccca cgaagtcatg aatacaaacc tatctaaaat
    16261 agctaaagta atacgtattc agttttgtaa gttttctaga tttcataaaa aatcgctcca
    16321 a
//
LOCUS       DAYYME010000060        16320 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000060 DAYYME010000000
VERSION     DAYYME010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.060"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..906)
                     /locus_tag="ACHBFW_001592"
     CDS             complement(<1..906)
                     /locus_tag="ACHBFW_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9317900.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNR"
     gene            1093..1584
                     /locus_tag="ACHBFW_001593"
     CDS             1093..1584
                     /locus_tag="ACHBFW_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFM9317901.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(1608..3983)
                     /locus_tag="ACHBFW_001594"
     CDS             complement(1608..3983)
                     /locus_tag="ACHBFW_001594"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFM9317902.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            4309..4935
                     /locus_tag="ACHBFW_001595"
     CDS             4309..4935
                     /locus_tag="ACHBFW_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM9317903.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            4958..5662
                     /locus_tag="ACHBFW_001596"
     CDS             4958..5662
                     /locus_tag="ACHBFW_001596"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9317904.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            5854..6687
                     /locus_tag="ACHBFW_001597"
     CDS             5854..6687
                     /locus_tag="ACHBFW_001597"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFM9317905.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            6884..8812
                     /locus_tag="ACHBFW_001598"
     CDS             6884..8812
                     /locus_tag="ACHBFW_001598"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9317906.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            8950..9405
                     /locus_tag="ACHBFW_001599"
     CDS             8950..9405
                     /locus_tag="ACHBFW_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFM9317907.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            9485..9613
                     /locus_tag="ACHBFW_001600"
     CDS             9485..9613
                     /locus_tag="ACHBFW_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317908.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            9875..10144
                     /locus_tag="ACHBFW_001601"
     CDS             9875..10144
                     /locus_tag="ACHBFW_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317909.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            10544..10798
                     /gene="rpsT"
                     /locus_tag="ACHBFW_001602"
     CDS             10544..10798
                     /gene="rpsT"
                     /locus_tag="ACHBFW_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFM9317910.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(10887..11933)
                     /gene="holA"
                     /locus_tag="ACHBFW_001603"
     CDS             complement(10887..11933)
                     /gene="holA"
                     /locus_tag="ACHBFW_001603"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFM9317911.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(11971..14244)
                     /locus_tag="ACHBFW_001604"
     CDS             complement(11971..14244)
                     /locus_tag="ACHBFW_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFM9317912.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(14237..14776)
                     /locus_tag="ACHBFW_001605"
     CDS             complement(14237..14776)
                     /locus_tag="ACHBFW_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFM9317913.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(14912..15130)
                     /locus_tag="ACHBFW_001606"
     CDS             complement(14912..15130)
                     /locus_tag="ACHBFW_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFM9317914.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(15127..15372)
                     /locus_tag="ACHBFW_001607"
     CDS             complement(15127..15372)
                     /locus_tag="ACHBFW_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM9317915.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(15488..16216)
                     /locus_tag="ACHBFW_001608"
     CDS             complement(15488..16216)
                     /locus_tag="ACHBFW_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFM9317916.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         4995 a         3171 c         2899 g         5255 t
ORIGIN      
        1 acgatttttt atttctggca atacgccagt tgttcgatac attttttgtt cgttttcatt
       61 tgcaaaccgg ccaaatactg aatgagttgg taaaatattg ataaatgcat cgtcgtcgat
      121 ttcatgaacg atctgatcaa gatcatacat ttcgtagcgg gtgatgacca tcatgatcat
      181 gttcccttgt ttttttgaat agccaccgac agacggcagt aatgtcattc cacgtaccat
      241 ccgttttgct agttcttctg ttacctcttc tggcttgacg gtaacgatca ttgctgttac
      301 tttttgatga ctcgtgtgga tggcatcaat tacttgagtc attgcataaa tggaaataat
      361 cgtatacaag gcactttccc agttaaagaa gaagcctgcg atgactacaa tgataccatt
      421 tagaatcagc atgtagtttc cgactgtttt tccagttgtt tgtgaaagta caagagagat
      481 gatatccatc ccaccagttg taaagcccat ttttaaagat aatcctacgc caagacctaa
      541 taaaacaccg cctactaggg cattcatcaa aatattatca gtgatctgtc cgactggaag
      601 cagcatcgtc atcaccgata cacatacaac attcacaaaa ctaaggatcg ttgctcgttt
      661 tcctactttg agaaacccta atacaaacac agggatattc aaaagaaaaa taaatgttcc
      721 tgtgtctatc ttgatatgaa accattcata taaaagcgag gaaatgattt gagcgatccc
      781 attcatccct gcagaaaaga cattggctgg aatcaaaaaa taatttaaag cgaccgcagc
      841 ggtcaatccg gttatcaaaa taaccactat tttctttaat ctttcatttt tatgatacgc
      901 agtcataatt ttactcacaa gcacctctcc cctactttgg tattccaaat gtaagaataa
      961 cagtttatct attaaaataa aatacttcga actcaaatga gaagaatttt agcaaacagg
     1021 taagaatctg ccctaatcct atgataagat agatgtatgg taaatattta tatgtatatc
     1081 aggagggttc ctatggcaaa agatgctagc ttcgacattg tatcagaagt caatacagaa
     1141 gaagtaaaaa atgcgattca gcaaacaaca aaagaattgt ccaatcgctt tgacttcaag
     1201 gattctacag tagaaatcaa attagagaac agcaatctag ttgtattgag cgatgatgat
     1261 ttcaaaatcg aacagatcaa agatgtatta tttggtaagc tgatcaaaag aaatgtgcca
     1321 atcaaaaata tccatttttc cgaatcagaa cacgctctag gtggcaaagc tcgacaaact
     1381 gccgatttgg tcaacggaat cgatcgtgaa aacgcgaaga aaatcactac agctatcaaa
     1441 aatgaaaaac tgaaagtaaa agcacaaatc caagaagatc agattagagt gacaggtaag
     1501 aaccgtgatg atctacaagc tgtgattgct ttattacgta aacttgactt accaatcgag
     1561 ttgcaattca ctaactatcg ttaatcaaaa aacgaggctg acgtctgtca ttcctcgttt
     1621 ttttgattta ctgcttcttt tgataaccaa aaacgctgta ttccttcttc caattcccca
     1681 acacttcgta cttctcttgc tgtttgccaa tgttgtggga ataaacgtcg ataagaaaaa
     1741 ctagaaaatc gttgatctgc caatacaacg attccttgat ccgccatgcc acgaatcact
     1801 cgaccaccag cttgcaagac tttgttcatt ccaggaagct gataagcgta agcaaatcca
     1861 aggtgttcct tctcatcata atatgatttg atcagttcct gctcatgatt catctgcggc
     1921 aaacctacac ctacgatcat actgccgatc aatcggtccc ctctcaaatc gattccttct
     1981 gaaaaaatcc cgccaagtac gcaaaatcca atcaatgttt cagcgggatt tgtttgaaat
     2041 tttgttaaaa atcgttcgcg ttcttcctca ttcatgtccg tttcttggat tagcacattg
     2101 ctgtcgggat attgctgtct gaatgcttcc accgcaagat ctaaatattg ataagaagga
     2161 aagaaaacga aataattccc gacctttcca tcagcaaaag catgaatcgt ctctactaat
     2221 tttgccagac tgtgttccct ttttcgataa gtcgtctcta ggtaatttgc gatgaaaact
     2281 tgctggtttc cttctgggaa aggactcggc agtttgtaag ccagactttt ttgtcccccc
     2341 agtgtttcct gataataaga gagtggtgaa aaactagcag aaaaaagaat actgcttttt
     2401 cctttatcca acgactgctc aagaaacaag cttggatcaa tacaaaactc ttttactatc
     2461 agatcgcgat acgttttttc gactgttgtt tcgtagtgat catcataaaa ttcactgacc
     2521 ttcaaaaaat gaagcaaatc aaaataataa ggaagcaatt gttcttgtga tgggtgctca
     2581 ggaaattctg ccagccattc tttaattttt tcgcttaatt gataacctgc ctttactaaa
     2641 gattcagccg gtgctttttg atgatgataa tcccaatgat cttcttttgc tatttctcgg
     2701 atcgaatcaa attcttttaa tagtttgttg aaacggcgat gaagtttttt gaattccttt
     2761 ggtatagctt ccttacttcg tttcgttttt tcataagata gttctgccga atacatctct
     2821 cgggaacgat tcaccaaatt atgtgcttcg tcaatcaaaa acagatagtc ctcgtttttt
     2881 tcatcttcaa aaaaacgtct taaataaaca gttggatcaa ataaataatt gtaatcccca
     2941 atgattacgt cacaccagat actgacatct aatgatagtt cgaaaggaca aagtgtatgc
     3001 tttcttgcat aagtttcgat gacttcacga gtgatctgat tctcatgatt caaaagatcc
     3061 cataaacctt cattaatccg attgtaataa ccattggcat atgggcactg atctggatta
     3121 caggtcgtct catctaaaaa gcagatcttg tctttcgccg tcaaagtgac actttttact
     3181 tcagctcctg tatcttttaa tgctgtcata gcatcttcag ctacttgtct agttattgtt
     3241 ttagccgtta gataaaagat cttttctccc tcttcttccc ctacagcttt caaagcgggg
     3301 aataacgtag agattgtttt tcctgttccg gtaggtgctt ctacaaacaa tttctgtttc
     3361 gtacggatcg ttttataagc agctaccgct agttcacgct gacctttcct atactcatca
     3421 tgggggaatt tcaaagccat caaagaggca ttgcgtacag tgcgccagtt ttcttgaaaa
     3481 acaagccatt ctttataatc ttcagtcagc tttttaaaaa aagtttctaa ctgctctctt
     3541 gtctgatgtt caacctttct cgtgatcact tcttctgccg tctggaagta agtcaattgc
     3601 agatttattt cttgtaaatc atgttcttga ctatagatat aggcatacac ccttgcttga
     3661 tgaaagaaaa gttccacttg ttctggttct aaatcttcaa atcgtggttc ggaggttttg
     3721 atttcatcta tataataaat accgtctctt ttgaaaatac cgtctgcacg gccttcaacg
     3781 atcaggtaat attcatctaa ttgaactgtt gtttttaaaa agacttcttt ttcatagttt
     3841 tctcccgcat ctttttggag cttgcggtgg attctcgctc cttctaatgc agtatggtta
     3901 ctttttttac gattgtcgat acttccacgt cgcaaaataa attctactaa gtgccgcaca
     3961 gatatttttg actgtttgat catttccacc acctctttct ctctaagatt acctgaaaaa
     4021 acctagttta gcaaaaagag ctctctttcc aaacataaaa aaacaccctg ctgattaggc
     4081 agggatatag gatatagaat ttggattttt ggatgaatct aatgtaacaa ggacatatta
     4141 aaatgctatg aaaacttact ttccttctct ttagaatatc caaatacttt tcatatagtc
     4201 tgataattca gataagtaaa gagaaataag tacctgtttt tgtttcctag caatgaatgc
     4261 gttatactaa tggatgaaag aagttttggc tatcaaggag gaatgcacat gcatatcgaa
     4321 agattaaaaa caggaatgat tgaagaaaac tgttatttgg tctacaacga agaagcactt
     4381 ttgatcattg accctggaga agatgcggaa cgaatcaaac aagaaatcaa aaaaacaaac
     4441 caacagccgg tagcgatttt gcttacccat actcactacg atcatatcgg tgcagtggaa
     4501 cctttacgcg cgtattatca aatcccagtt tatgtcagcc cactagagca aaaatggcta
     4561 ggtgatccga ttttgaatct ttctggactc ggtagacatg atgatattgc tgatgttatt
     4621 gtctcgcctg cagaatatga atttgaaatg aaaacttata ctttaggaaa catgtctttt
     4681 cgcgtcgtcc ctaccccagg gcattctata ggaagcgtca gctttatttt tgatgatttt
     4741 gtcgtttctg gcgacgccct gtttagagga agtatcggta gaaccgatct gtatacaggg
     4801 aatttagaac aattacttca tagtattcgg acccagctgt ttgtattacc tgatgaattt
     4861 gcagtctatc caggacatgg cgatgcaacg acgattgaac aggaaaaaag gacaaatcct
     4921 tttttcaatc gttaaaacga cttaggagga gaataaaatg acagaaacta cactttatat
     4981 cgtacgacac ggaaaaacga tgttcaatac aattgaacga gtacaaggct ggtgtgatac
     5041 acccctcaca aagcaaggac aagaaggcat tcattattta ggtcgcgggt taaaaaatgt
     5101 cgacttttca tttgcttatt ctagtgacag tggtcgtgca atcgaaactg cccgtattat
     5161 tctaggagaa cattctaagg gaaaagaaat tccttatttt attgaaaaaa ggattagaga
     5221 atggtgtttc gggtctcttg aaggtggata tgacatggaa atgtggggtg tgatccctcg
     5281 tgtattgaat tttaaaacat atgatgaaat gtttaccaca gaagtttctt ttgaacaaat
     5341 tgctaatgct gtatacaact tagataccgc tgggtgggcg gaaacttatg aaacattgcg
     5401 agaccgtgta tggacaggat ttgaagatat cgcccatcac tgcgaaaaaa atggtggtgg
     5461 aaatgtttta gtcgtatctc atggtttgac gatcgccttt ttgcttaatc tgatcgatcc
     5521 taaacagcct gttcgtgctg ggcttttaaa tggcagcgta acaaaggtca cttatgcaaa
     5581 cggtcgattt tctatccacg gcatcaatga tacgtcttat atcgataacg ggaaaacaca
     5641 tagtcgcgac tcgtttctat aatggggata ttttaatttt taccagagaa tcataaaagg
     5701 caaagtctga acgagggctt tgtcttcttt tttctcttcg tgtacttatc tgctttattt
     5761 ctcaagtttt tcttaatatt aaaaaacaga ttgggtttct caaaaaataa cggtatacta
     5821 taagatgtat cttaattggg aaggaaggaa tttatgtttt ttgcaaatat tatcaaagca
     5881 gtgattctcg gtattattga aggaataacg gaatggctgc ccatcagtag tactggtcat
     5941 ttgatcctag cagatgaatt catcaaactg gatgccagtt cacaatttat gtcgatgttc
     6001 aatgtcgtta tccaattagg ggcgatcttg gctgtcgtcg ttctttattt tcacaagtta
     6061 aacccatttt ctccaagtaa atcaacggta gaaaaaaaag atacgtggac actttggttt
     6121 aaagtgatcg ttgcttgcat accagccatc atcattggtt tgcctttgga tgattggtta
     6181 gaagcaaatt tccacaagtt cttaccagtt gccattgttt tgatcgttta cggtattgcc
     6241 tttattattg tcgaaaaacg caacaaaaac aaaacacctc gttggacttc tctgaatgac
     6301 tttacgtacc aagcagctct tcttgttggg ttattccaag tcttggcact tgttcccgga
     6361 acatcccgtt ctggcgcaac gatcctagga tctatcttga ttggtgcttc gcgttttgtc
     6421 gctacagagt tttcattttt cttagggatt cctgtcatgt ttggcgcaag cggcttgaag
     6481 atcgtcaaat atttagctga agggaattca ttcaaaatgg aagaaactgt tgttcttctt
     6541 gtaggaacat tcgtatcttt tgtggtttcc gtcttagcta tcaaattctt actaagctat
     6601 ttgaagaaaa acgactttac cgcttttggc tggtatcgta ttatccttgg tatcattttg
     6661 attggttatt ggtttattgc aatgtagaaa aaaccagaac acttctctta tttgagaaat
     6721 gttctggttt tttattttct ttaataataa tcattataaa taataaaaac aacatcaagc
     6781 ttaatcacca aatgataacc attatcgttc tcatttagct gttattatta tcaattacat
     6841 ttttattatg ctatccttgt tttgacaagg aggtctaata acgatgacta atcagaaaaa
     6901 agcttttgca gcaacaatcg gctgcctttt atttatgatc ttcggtttta tcttcgaccg
     6961 attctctctt accatcaccc cactttttta tatcagtgcc attatcatcg gtggatataa
     7021 acaaacatcg gaaggcttga aagaattgtg gaatgatcgg acactgaatg tggatttatt
     7081 gatggcttta gctgctattg gtgcctgttt gatcggtaat tactttgaag gagcaatgtt
     7141 gacattcatc ttttgtttaa gcggtgcatt agaggaatac acaactaata aaagtcaaaa
     7201 agaaattact gctttgatga atcttcaacc acaaaaagcc caattattaa aagaaaatgg
     7261 acaaatgcaa gaagtagatg tttctcaact aaaggtggat aacttggtat ttgtcgctaa
     7321 aggagcagct gttccgatag atggcttttt agaaagcaca caggcaacga tcgatgagtc
     7381 tgcattaagc ggtgaatctg tgcctgtaga aaaatattta ggcgatgaga tgtatgcagg
     7441 aacgctgaat caaggaaatc ccatcacgat tcgagtaaca aaaacgagtt ccgaaactgt
     7501 ctttgctaag attatccaat tagtagaaga agcgcagaat acgccaactc aaacagcttc
     7561 atttatcgaa cgaatcgaaa ataattatgt aaaattgatt ttgcttgctg ttcctctaat
     7621 gatccttctc ccccactttt tcctaggttg gagttgggat gaaagctttt atcgaggaat
     7681 ggtattatta gttgtggctt ctccttgtgc gcttgtcgca tctgctacac cagcttctct
     7741 agctgctctt tcaaattctg caaaaaatgg gattttaatc aaaggcggta tccatttaga
     7801 aaagctttct gaattaaaag cgattgcctt tgataaaaca ggaacgctca ctaaaggaaa
     7861 accagttgta actgacagct ttttctttga tgaaaaggat ttggcacaac aacttcttgt
     7921 tgctatggaa caaaaaacga cgcatccact tgctcatgcg attttgcaat atttcgattg
     7981 cacaatacct gaagaaatcc aatacttacc aatagaagaa attactggtt ttggattaca
     8041 aactacctat ttaggtgcgc aatggaaagt tggcaagcac gcatatgatc ctgagacgat
     8101 gataatcagc aaagagattg cggaaatgat cgaacgtttg gaaaatcaag gcaaaaccgt
     8161 gatttatctt tctaaagatc agcagttaat tgctgtttta ggtctgctag atattccaaa
     8221 agcaaatacc caacaagtaa tttcctactt taaatcccaa aacatccata caagtatgat
     8281 aaccggcgac cattctggca cagcaaaggc cattgctgaa caagtaggga ttgaacatta
     8341 ctatgcaagc tgtactccgg aagaaaaaac acaactagtc aaaaaagaga atgaacttta
     8401 ccaagtaaat gcgatggttg gtgatggagt caatgatgcg cctgctttag cagccgcttc
     8461 tttaggtgtc gctatggggc aagggacaga tgccgctatg gatatcgcag acatcgtctt
     8521 aatgaaaagc gacttaaata aatttgtcta cagtcatcgg ctatctttaa aaatgcggcg
     8581 gatcatcaaa caaaatatta ttttttcgat tcttgttatt ctcttgttga ttgcttctaa
     8641 cttcctgcaa tttttaaatc tacctcttgg agttgtagga cacgaaggaa gcacaatatt
     8701 ggttattctg aatggcttgc gtttactaag acctttacct gatctttctg attcttcaaa
     8761 aaaatgtaag tcttgtccac tatacaaagc gcggctgtct tctgtcaaat aagcaaagtg
     8821 tttttttatg attttgttta ttttatgaaa aaaacagctt atgaataggt cttgaaggat
     8881 gaattcctac ttttgtaact ttctaaaaac atgttatgat tgtttcgaag aaagtgagga
     8941 atatatttca tggaaagttg gcttttttta ggaattattt tacttgtagc aattttagct
     9001 aaaaatcaaa gtctgatgat agcggcagct tttgttttga ttatgaagct cataccagcc
     9061 tcccgttcct ttttcccttg gcttcaaggc aaaggaatca atattggggt gacgatcatc
     9121 acagcggcta ttctaatccc tatcgcaaca ggagaaatcg gtctacgtga tctattagaa
     9181 gcctttaaat cccctattgg ctgggtagct gttttttgcg gtgggctagt ggctgtctta
     9241 tcttccaagg gtgtaggatt gattgcttct gatccacaaa tcactgttgc acttgttttc
     9301 ggcacgattt taggtgttgt cttcttaaga ggaatcgccg ccggaccggt cattgcttct
     9361 ggtatcactt actgtatctt acaagtattt gctttaggaa aatagaacaa attttgtcta
     9421 ttttaggaga ttcttaacac ccccaattta ctgatccgtg atatactgac aacgaggtga
     9481 tacaatgttc gaaaaattaa agtatttcat aggaacaatc ggctggtttc ttctagcggt
     9541 acttttgatt ctgggcttag cttatctatt atttcctgat ttgatacaaa atgtgtttga
     9601 tactgctttt tgattgacct attaaaacga tataggggat gctcctgcga tgcaggggct
     9661 ttttttaatc aatgttttgt aataatttag gccgtgccaa tgagacttcc tatagaattc
     9721 gatgctcttt tttctttttg gctcttctgc tcttttttgt tatactagat tacagtggac
     9781 aaaagaaaga atgatcgcat ccacttcaac gaatatcaaa atggaagaat tagtgcctgt
     9841 tttcaggtta ctttgaaaag aaaggaagac gcttgtggaa acttttgaag gtcttttaat
     9901 tgatacgtac gttttacaag aacagccaat gatgctgact gggatcaact tgcagcttga
     9961 ggaccgtgtg atcaatggcc tgcttaaaga tcgctatttg gcaaaatata tcttacgttt
    10021 gcccctaaat aaatatgtgc tggaagtaag tggcaaatgg aaccgtcgga accagttggt
    10081 cataaaaaaa atgttgataa aaaatatcga tgaatacatt aaaataatcg gtacaccaga
    10141 atagccttta ctgatcaaag gatactggtt ataaaataac tgcagtttct taatagaaaa
    10201 acaaagaaca acagggaatc gtatcccttt tcaaaaaaaa ctttgcaaac ggttctcttt
    10261 gttatataat aacttagtac caaacattcc cattcaaact ttgtttaaat agataaataa
    10321 aaatctagtt tggtttgacg ctattgcctc ccacaatagc gtctttttga tttttttcag
    10381 cttgtactat ccgtttttac tgaaaaaaaa tcctaataaa agactgttaa ggaaaaaaac
    10441 ttgtcatgtt catattgcaa agcacgctgc ctttaggtat aataatgaat gttgaattat
    10501 gcggtaacac gcgtaagaac ttaagttatc ggaggtgaaa ctaatgccaa acattgaatc
    10561 tgcaatcaaa cgtgttcgta caagcgaaaa cgctaatgtg aaaaattcat ctcaaacaag
    10621 cgctatgcgt actgcaatca aaaaatttga agatgctgta gcttcaggtg ctgacaacgt
    10681 agatgcgtta tataaagaag ctgttaaagc tattgatatg gctgaatcaa aaggtctaat
    10741 ccacaaaaac aaagctaacc gcgacaaatc tcgtttaagc aaaaaaatcg ctaaataaaa
    10801 gaatcggctt cggccgattt ttttatttgt caaaaaaagg acaaaaacaa acgaccttcc
    10861 cgcatgcttt gccctcgtct ttcttcttat cgatttctta acaactgccc tgtgcgcaaa
    10921 acgaatagtt gaaaacttaa ttctttttcg atttgtcccg ttttgatttg ataatccatc
    10981 tcaactaatt gatcaaacaa ctgtaccagc aaatgttcat cgaatttttt tacttcctgc
    11041 atggcaagtt ttacacgata tggatggatt ttcaatgttt cagcaatatt tgcctgctga
    11101 taaccaatct ttacaagaaa ttttgtctgt aaaaataaac gtaattgaga aagcaaaatc
    11161 gcattaattt tgattgtttc ttccccttgc ataactaaat cttcgtataa acgcaaagct
    11221 tgttccgtct tcccttttaa aatatattgg gtcatgtcaa aaatattatg ttctaacgtt
    11281 ttaggaacta attcttctac ttctcttacc gtgatttgct gttgattttg tccataaagg
    11341 accaatttct gtaactctct catcattttg gataaatcta agtcggtcaa cctcaagaat
    11401 aaatcaattg cacttcgttc cattttgatt gaggaattct ctaaggtatt catcagatat
    11461 tgacgtactt ctttttcatt caactgttgg acattgatga taattgctgt ttttttcaat
    11521 tgtttcgtta cttttttccg ttcatccaac ttttcatatg gcgcaaagaa tactaataca
    11581 gtcgattcta atggttcttt caaataagcc gtcaataaat cagtattttg ttcgattcca
    11641 ttgtttactt tttcacctgt taaaaaataa ggattctcta cgaatactaa ccgttgatct
    11701 ccgaaaaacg gcaacgtctc tgcttcagct actacctcat ctaaacttgc attttccatg
    11761 tcaaaactta aaaaatttaa gtcatcttcg ccctctaatt ggattttttt gacaatttct
    11821 gtccgaactt gatcttggag aaacttttct gttcctaaaa tcaagtaaca agatgcaagt
    11881 ttttctttac gtattttttg taactctgct tgtatactca aattactcac cactttcaaa
    11941 aattatccta ccatgagcag atgagaatta ctagtctatc attgtttcca ctagcccatg
    12001 aggatgaaaa actggatgcc agcgataggt gatcattcct tgttgatccg ttcgccatgt
    12061 ttttatctga ttttcttcta gtgtctccaa tacttcgctg tgcgggtgac cataacgatt
    12121 atttacgccg caggatataa tcccatgttt tgcttcaata tgggaaagaa aatctgtgtt
    12181 acttgacgta cggctaccgt gatgacctaa tttgactaca tcggctttca gattaggata
    12241 aatcaaaagc aattgttttt ccattgcttg attcaaatct cctaaaaata aaaagcgcct
    12301 attgttatat tccatccata gaccaatcga atcttcattt cttccctctc ccttttcaaa
    12361 aggagtcaat acataaagat cataataacc gctgatcctg tccccatgtt tgatttcaat
    12421 gatctcagta ccattttttt gagcttcaga taaatgcctt tgtaatggct catttcgtcc
    12481 agcgccccaa ccgatataaa tttttttgat agtaaaatgc tgagataacg tgggtaattc
    12541 tcccacatga tccgtatcgt cgtgtgtcaa gatgagttta tcgattacac ttatcccctg
    12601 accttttaga tatggaacga tatttctttc agcaggagga cgtatcatac cttgcgccca
    12661 cttttctctt tcaaagtgta actgacctcc agtatcgatc aaaatcgttt cttttcgaaa
    12721 aggactttgc aaaaagatac tgtctccttg accgacatcg ataaaagcga tcgttgaaga
    12781 aaagcgtaaa aatggcaaag tcaaaaaaat aaaagaaaaa agaccaacat aaaacaagtg
    12841 tttcacaggt tgttctttca acctatccat acaaatcagt aatccgaaaa tatacatccc
    12901 agatagcaca aggggcaaac gtcctataat gagagacgac cctgaagaaa aatcgattat
    12961 tcgctcaaat aagattaatc cctcttctat cagcaaaagt aaagtttccg gcagccagcg
    13021 attgaagagt gtgagaaata acacaccagg aagaaaaaga aacttgaata ccggtgcaat
    13081 cagtaatgta aaaagactac ctaaaatagg ccactcataa aaatgccata caatcaacgg
    13141 catcgtgatc atagtaaaca ggacgtgaga ggccgttttt ttcaaccaat ctccatcaaa
    13201 agaaatcagg ataaacaaaa aactcatgaa aaaactaagc tgtccacccg catgaaaaag
    13261 aaatcgagga tcaaatagta aaagaagcca aaagacaact gcaaataagt ccatggtgga
    13321 ataatgctta ttcagcaatt tgactagcaa tcgcaataaa aacagcaaac tagcacgcaa
    13381 aacagaaata gaacttcctg caatagaaac ataacagata gttagtagac tcaacggaat
    13441 aatactgacg tgtagtggcc atccgatacg tcgaaacaaa taatacagcc accctaaata
    13501 aaattggatg tgtagacccg atagactgaa cagatgcagc aaaccactct ccctaaaaat
    13561 ttcttctggt tctctgaatc ctgcatcttt atagccccat aataaagaat taatatatac
    13621 acttgttttt tgcggaaatt cagattggac aaaggagata aaagatcctc tttttctctg
    13681 aagccagttt cgccagtgat tttgcatggt gatctgttca atcttttgaa tttggaaccg
    13741 tccagaaatt ctatggttcc tgaagtaagc ttcttggtca aaattataaa gattccgctt
    13801 ttttgctgga gagatgaact cgccttcagc agtcaattct attccctttc ctcggaagtt
    13861 aagccatgtt ttcaattctg cttctgtgtt tacttgataa gaaagctgga gcttttgttt
    13921 attctctgat aaccttcctt ccattgtcaa aaaattacca tttgcacgaa tcgtatctgt
    13981 aaatacagta atttgcgcag atgttgtttt ttcctctaaa aatgaatact cttgtagcct
    14041 ataagaaatg gataccgtta tgctacacaa aatacacaag tacgcgataa aacgtccttc
    14101 ttttagcgaa aaccataaaa aaattccggc aagaagcaaa cgataacccc aattgtcagt
    14161 gtaatacaac aaacagataa tcatcgacgt aattgccgga aaaatcaacc gatttcgaac
    14221 atgcctttct aaaacgctag acatgttttc ctgttaattc attttgaagt tttgaaaaat
    14281 gctgtgagtc taaagcaact tggtgtacag atacacctaa ttgttcgatc aacgcttggg
    14341 cataaggatc atttcgatag tcatggagat aattgatccc tttcacacca gcttgcaaca
    14401 aagcctttgt acaggccaaa catggaaaat gtgtgacata gatttctgaa ccttccgtcg
    14461 ggataccaag ttttgcacac tgtaggagtg cattcatctc tgcatggatc gttcgcaagc
    14521 agtgtccatc cacgacgtaa cacccttcat ctatgcagtg gacatctcca ctgactgctc
    14581 cattatagcc gccagcaatg atcctttttt ctcgaacgag tgtcgctcct acttctaaac
    14641 gtgtacacgt actacgtaaa gataacagta ctgcctgagc cataaagtac tggtcccaag
    14701 gaatgcgttc attatgtgac ttatcagtaa ctttttcttt attcgtatcg catgcttttg
    14761 aagatttttc taacatgttt tatattcctc cgttttttca agttgattaa aaagtactca
    14821 ttgcttaaac aaaatattct ttaaatataa cgaatcatcc tgtctgacaa aagatcctac
    14881 ccttcactgc cgaaatgaag tccgtcatcc attagcccat tataaaaatt cttcgaatcc
    14941 atttgttcaa gaaattccgt ataaaaatcg atacttcaca atcatggcgt tttgctactt
    15001 ctgtgagaac agagatatat tctttgatca acttgttatt ccgatatttt tgttttgtct
    15061 catcgactgc tggtggcgtg ataaagaaag catgatcaga actatagtct tttttcagct
    15121 gaactatcat ccactgcaga ttttttggta cgtttcttta gaaagttgtt tatgctctgc
    15181 tagatcattc gtcccaatga gaataaatac tttatctgct gtatagctaa gtacatcttt
    15241 ggcaaagcgt tctcgtagag cttgactgtt attgccgctg acagccgtat tcataatatg
    15301 aatcccagat attttatttt ggagatagac attcagcatt ggttttttta atgtttcttt
    15361 gcgaacaatc aagctatttc ctatccaaaa atactacctc ctcgtcctgc caagctctta
    15421 gaattggatt attcttagta tccattagcg tccatttatt gctcgtgcca gtcgattttg
    15481 aatcattcta tttaattgta acgaagtttt ttagtttttc aactgtcttt tcaccgatac
    15541 cagaaatctc ctgcaggtct tccactttct ggaaggatcc gttttcttct ctaaatcgaa
    15601 tgatttcttg cgctttgact gatccgatcc caggaatctg ctgaagttct gatggagtgg
    15661 ctgtattgat atttattttg ggaggctcgg aatctgctgt aggctgtttt ccttccgagg
    15721 atgccaactg atcaagatgt tctggcagct cttcacctac agcagggata tataggactt
    15781 gctgatcttc tatcttcgca gaaaagttga ttcgatcgct atccgcttct tctaataatc
    15841 cacctgcacg ctttagtaca tcatagaccc gttcttctga agaaaaggaa tagataccag
    15901 gctccttcac acttcctttg atatcggtgt acatgacctt tggacgagca tcctcttcta
    15961 catcgtttcc ttcagctgat ggatcttctc cactggaact aaacgaagaa agtatttctt
    16021 ttgcagcagt ctcttctgtt tctctataaa atagcaaatt tgataataaa aaaccacttg
    16081 cgatacaaaa gataagaact atcatagaca tccaaatgat caatttgggt gaagctagta
    16141 ttttcttgct gattttttct atcattgttc ttttttcaaa gaatgaccag taaaaatcat
    16201 tcattactcc tttcatttaa aattgaagaa acagtgagct ctggcaaaat cgtcccttca
    16261 acatctaatt acgtcaattt tttcttttgt ttttattttt atcgtgaaga tttttgacga
//
LOCUS       DAYYME010000061        15644 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000061 DAYYME010000000
VERSION     DAYYME010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.061"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            87..653
                     /locus_tag="ACHBFW_001609"
     CDS             87..653
                     /locus_tag="ACHBFW_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9317917.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            798..885
                     /locus_tag="ACHBFW_001610"
     tRNA            798..885
                     /locus_tag="ACHBFW_001610"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:830..832,aa:Ser,seq:cga)
     gene            1221..2195
                     /gene="bsh"
                     /locus_tag="ACHBFW_001611"
     CDS             1221..2195
                     /gene="bsh"
                     /locus_tag="ACHBFW_001611"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM9317918.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            2520..4415
                     /gene="asnB"
                     /locus_tag="ACHBFW_001612"
     CDS             2520..4415
                     /gene="asnB"
                     /locus_tag="ACHBFW_001612"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFM9317919.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(4610..5056)
                     /locus_tag="ACHBFW_001613"
     CDS             complement(4610..5056)
                     /locus_tag="ACHBFW_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM9317920.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            5284..5433
                     /gene="rpmG"
                     /locus_tag="ACHBFW_001614"
     CDS             5284..5433
                     /gene="rpmG"
                     /locus_tag="ACHBFW_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM9317921.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            5521..6039
                     /locus_tag="ACHBFW_001615"
     CDS             5521..6039
                     /locus_tag="ACHBFW_001615"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM9317922.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(6116..7072)
                     /locus_tag="ACHBFW_001616"
     CDS             complement(6116..7072)
                     /locus_tag="ACHBFW_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9317923.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            7238..8044
                     /locus_tag="ACHBFW_001617"
     CDS             7238..8044
                     /locus_tag="ACHBFW_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9317924.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8041..9030
                     /locus_tag="ACHBFW_001618"
     CDS             8041..9030
                     /locus_tag="ACHBFW_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM9317925.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            9027..10031
                     /locus_tag="ACHBFW_001619"
     CDS             9027..10031
                     /locus_tag="ACHBFW_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM9317926.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            10163..10705
                     /locus_tag="ACHBFW_001620"
     CDS             10163..10705
                     /locus_tag="ACHBFW_001620"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFM9317927.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            10725..11423
                     /locus_tag="ACHBFW_001621"
     CDS             10725..11423
                     /locus_tag="ACHBFW_001621"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM9317928.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            11444..11656
                     /locus_tag="ACHBFW_001622"
     CDS             11444..11656
                     /locus_tag="ACHBFW_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFM9317929.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            11656..12618
                     /locus_tag="ACHBFW_001623"
     CDS             11656..12618
                     /locus_tag="ACHBFW_001623"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFM9317930.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            12636..13031
                     /locus_tag="ACHBFW_001624"
     CDS             12636..13031
                     /locus_tag="ACHBFW_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM9317931.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            13194..13559
                     /locus_tag="ACHBFW_001625"
     CDS             13194..13559
                     /locus_tag="ACHBFW_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFM9317932.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            13556..15367
                     /locus_tag="ACHBFW_001626"
     CDS             13556..15367
                     /locus_tag="ACHBFW_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFM9317933.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(15429..15596)
                     /locus_tag="ACHBFW_001627"
     CDS             complement(15429..15596)
                     /locus_tag="ACHBFW_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFM9317934.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
BASE COUNT         4863 a         2632 c         3275 g         4874 t
ORIGIN      
        1 gttttgagta aaatgtttgc aaaaaatgct aaaaataggg tacaataaaa caacatatac
       61 tgcagtaaag aagtggggga gttttaatga gaagaaaagt ctataccaag gatcaaattc
      121 taaaagctgc ttatgatgtt gttgcaaagg aagggtttaa aggatttact gcgagaaata
      181 ttgctaaaaa gatggggatt tcgacacagc cgatttatct ggaattcaaa aacatggaag
      241 atttaaaaaa tacgttgctg gattctatct ttgaacaact aaaagaagat gttttcccta
      301 ttgaacgtac tggtgataaa atcattgatc tcggtttgaa ctatatctac tttgcaaaaa
      361 agcatcatag tctttacatg gcattgtaca ttgatgaaca tggggacgga aagaaaatgc
      421 atgatttttc atacaattat ttccgtgaaa tagttaaaga agatgaaaga tacaatcaat
      481 tatcggatga atatgtcgat gctctgcata ctggtacatg ggtagcagtg actggcttag
      541 cgtcattgat gtcatcaaac atcatgcatc caacagaaga acaaatcatt gcactgatca
      601 aacggacaat tgactcgatt ttagcattag ataatcctga aataatcaga tagtgttcta
      661 tcagtatgaa tttgatcggt aagtccgttg gctgcaaact gtcaatggac ttttttgcta
      721 aaaaaagcga tttctgttat ttatctatta gaaataggct tgacatttcg tgaaaaaagt
      781 ttaaaaatag tacttacgga aggttggcag agtggtaatg caccggactc gaaatccggc
      841 gaaccggcta ataccggcgc gcaggttcaa atcctgtacc ttccttatat caacgattag
      901 aggttgtgaa gaagctgtcc tgcatcaagt aataagaacg gccaaacaaa aatagctttt
      961 cctatttttt cgtttggttg ggcttattac cgcagatgca agcttttgaa caccgtttgt
     1021 tcggaataag gggctgtgac aagacttttg tcacagcccc ttattgttgt acttttaacc
     1081 tacaatattt tttgattccc cctgagtact attttttata aatgctagta aatcaatctt
     1141 ctttggcctt ttttttgata aaaaaaaata ctagatgtaa gataaaagtg taaaaacgat
     1201 ttgttttagg agggaaaacg atgtgtaccg caatcactta tgcaacgaaa gaccattatt
     1261 ttggaaggaa ttttgattat gagatgtctt ataatgaagt agtcactatt actccaagaa
     1321 attatcgttt cgattttaga aaggtaaaaa atttagataa acattatgca atgataggaa
     1381 tagcagctgg ggtatccaac tatccactct attatgaggc tacaaatgaa aaagggttga
     1441 gtatggcagg actgaatttt cctggcaatg ctgattataa agaattacaa gaaggaaaag
     1501 ataacgttgc accatttgag tttattccat ggatactagg gcaatgctca accatagacg
     1561 aagctaaaga attattggct actatcaatc tagtcaatat cgattttagt gaaaaactac
     1621 ccttgtcacc tttacactgg ttattagctg ataaagaaaa atcgatagta attgaaagta
     1681 tgaaagatgg acttcattta tatgataatc ctgtcggtgt gttgacaaat aatccgccat
     1741 ttgattatca attatttaat ctgaacaatt atcgctcgct atcaaatgga acaccagaaa
     1801 atcatttttc aaaccagatc agcttgaatg tttatagtcg tgggatggga ggattgggct
     1861 tacctggaga tctctcttct gtttctcgat ttgtaaaagc cacatttact aaaatgaatg
     1921 ctgcatcagg tgattcagaa tctgaaagta tcagtcaatt ttttcatata ctaggctcag
     1981 tcgagcaaca aaaaggagta tgcgatactg gagaaggaaa atacgaatat acgatttatt
     2041 cttcttgctg taacgttgat aaagggattt attattatcg tacgtatgaa gacagtcaaa
     2101 ttactgctgt aaatataaat aaagaagact tggatcgtca tgaattggtc agttatccag
     2161 tcataaaaga gcaacagata aactatgtca attaattaat gaatagatcc gtttggtatc
     2221 atattttctg atacagtcat aattagacac gatctttagg tcgtgtcttt ttttgctgtt
     2281 ttttttggaa atagcatcca ctaatatttt tttaacacga agagaataag gtagcctcaa
     2341 aaaagaaaac atggtaaatt agatattggg actttatttt ctgtaaaaac agaaactttc
     2401 atcttttgtt ttcacgtgac gaaattgtaa gagagaaaat attcgtccgc tatttattct
     2461 atgataagat gcatatgaat gaaataaaca gtaattttat attaggatgt gaaagttaaa
     2521 tgtgcggtat tgtaggtttt gttaacgaca aggacaacaa aaaaacaatt attaatacaa
     2581 tgatggatcg aatcgttcac cgtggaccta atagttcagg agaatatatc gataaacatg
     2641 ttgctttagg attcagaaga ttaagtatta ttgacttaga gggcggtact cagcctattt
     2701 acaatgaaga tcggacaaag atcattattt ttaatggcga aatctataat taccagcctt
     2761 tgagagaaga attgattgca gctggccacg tttttcaaac acatgcagat acagaagtcc
     2821 ttttgcatgg ttacgaagaa tggggaacag aattattgca aaagatccgc ggaatgtttg
     2881 cttttgctat ttgggataat gaaaagaatg agttattcgg tgcaagagac cactttggga
     2941 tcaaaccata ttattatgca gaaatgaacg gaacatttat gtttggttca gaaatcaaaa
     3001 gttttctgcc tcatcctgat tttaacaaag aattgaacaa agaagcgttg aaaccttata
     3061 tgactttcca atattctcca ttaaacggcg aaacattctt caaagatgtc tatcgtttgc
     3121 ctgaaggcca ttattatacg tataaagatg gaaaactaga cattcagcaa tattgggatg
     3181 cagattttga aacaaaagaa acgcattccc gacaagaatg gatcgaaaaa atcgacgaaa
     3241 ccgttcaagc ttctattgaa gcacatacgg tcagtgatgt agaagttggt tctttcttat
     3301 ccagcggggt tgattccagt tatgtaacct ctgtattgaa accagatcat tctttctcaa
     3361 tcgggtttga tgataaaacc tataatgaag cgattgaagc cagaaaacta accgaactat
     3421 tggacttaga taatacagca gcagtgatcg acggagacat gtcgttcaaa gcattccctt
     3481 tgatccagta tcacttagat gaacctgatt caaatccttc ttgcgtacct ttgtactttt
     3541 tagcaaatct tgcttcacaa agtgtgcggg tagtccaatc tggagaaggg gcggatgaac
     3601 tgtttgcggg ataccaaacg tatggattcc atacgaattc aaaattcatc cgtgtgattg
     3661 ctcaaggatt gaaaaaatta ccaaaaggaa cacgctataa tttaggacgt aaaatcggaa
     3721 aaatgaaaaa tttccatgga cggatccatt tatatgaatc acttgctcct gcaaaagaat
     3781 attttatcgg tcatgctcga gtgttcgaag aatcagaagc atcagaagtg ttaacaccaa
     3841 aatatcaaac agcaccatct gtggatgaaa tcatgactgt tcattacgag aaaacagaag
     3901 gaatcaaaga tgaagtcaat aagatgcaat atgtcgatct tcatcaatgg atgccaaaag
     3961 atatcttgtt aaaggcagat aaactttcta tggcaagttc tttggaagtc cgagtacctt
     4021 tattggatat cgaagtcatg aaattggctc aacaaatacc aagtaagttt ttattgaatc
     4081 aaaacaacac aaaagatatt ttcagacaag ctgcgaacaa acatcttccg gaagaatggt
     4141 cgaatcgtgt gaaattagga ttccctgtgc caatcaaagc ttggttgaaa gaagaacacg
     4201 gctacgaaca agtcaaagca ttattcgaag cagattttgc aaaagaattt tttgatcaag
     4261 aaaaaatcat gaagttgcta gatgaccatc acgaaggacg taaagaagag caaagaaaaa
     4321 tctggacaat cttcagtttc ttgacatggt ataaagtcta ctttgtagat gaaacgattc
     4381 cgcaagcaga agcaatcgac tatgtgactg tttgatgaaa atcaaatatc aataaaaaag
     4441 aggttttctc ctaccgttta acactgagtg ataagaaaca atttcaaaaa acagctcttc
     4501 ccgttttata gatattatga cttatggttc ggagttggca gaggaacacc attgtttcga
     4561 acataagggg gttgtgacac gattttcgtc acaacccctt tacatatgtt caataatcga
     4621 tttctttttc ttttgtccca tcttttttat aaatgaacaa agttgattct ttgttttgtg
     4681 ctacttgctt tcctttttcg attgcttctt tttttgtatc aaaatggttg cttgcctttt
     4741 ttgccccttt agatttcacg atccattgtt cttcctcata ttctacgatc acatctgaat
     4801 caagcagttt tccagcacga ggattcgtat cgtgttcatc cgtcttgctt ggattttttt
     4861 ctttttcgaa agttcgtttt tctgattcac tagcatcttc ataccatttt tttgcttgat
     4921 caatggcaat tgggatcgca cggttatcag ggtacccttc atctaataat gcatttgcga
     4981 tgtcgattgc tttttttctt gtcaatttat ctaagttttt cattgatgca ggaaagtcct
     5041 tcatgttcca tggcatatta gtatccccct ttattaaaaa acataatcta tataaacaag
     5101 tgtaagggta tttatacaaa aagaaaaaga ttttgtttga gaagtaaaac cgttgtattt
     5161 atcgtcaagt agttttactt aacaaaatcg tgaaaaacac tagcaatcta gaaaaaatca
     5221 tggtaagata atcaagtctg agagtcatct tagaggattc ttgtaaaaat gggagggaaa
     5281 aacatgcgcg taaacatcac tttagagtgt acttcttgta aagaacgtaa ctatctaaca
     5341 agcaaaaaca aacgtaacaa tcctgatcgt ttggaaaaac aaaaatattg cccacgcgaa
     5401 cgcaaagtta ctttgcaccg tgaaacaaaa taatgttttt aaagttctca attccttgct
     5461 ggtcaaagaa ttgagacttt ttttatctca aaacatctaa aatcataagg agcggataat
     5521 atgtttattt ccatgtgggc acaagataaa aatggtctga tcggcaaaaa tggtttgtta
     5581 ccatggcgat tgccaaatga tatgcgtttc tttcgcgaac atacaatgga tcgaatttta
     5641 gtgatgggaa gaaagaccta tgaaggaatg ggggatcttt cacttcctta tcgccatatc
     5701 atcgtgttga caacgcaacc aaattttcgc acgaaagaaa atgcggaagt aatgcattcg
     5761 atcgatgaac ttttgtcgta tagtcaaacg atctcagaag atatctatgt ttcgggtggt
     5821 agtcggattt ttcaagaact tcttccttac actggtcgga tctggcgaac attgatcgat
     5881 agcacgtttg aaggcgatac gtatattggg aatattgatt tttcagactt cgcccttata
     5941 gaggagtatg aggggataac ggatcaagaa aatctatatg cccatcgttt tcaaaaatgg
     6001 gaaagagtaa aaaatagggt tgcttcaaaa gaagaataaa atctatgcgt ctagtttgtt
     6061 aataagaaaa aaagcatctc acgactaatc gtgaagattc tttttttagt taaaatcaat
     6121 ttagtttttc tttgatatca ttgaccattt cctgactggc aataggtccg ttatatagcc
     6181 aactagattc aggtccgaat tgcagtacat gattgtctgt cactgcttta agattcttcc
     6241 agcttggttc attgaacatg gcagcatctt tgtcactgtt cacaaggatg atgtagtcag
     6301 catctaattc agctagcttt tcagaggaaa cagcagacca gtcagaagtg gcttttttgg
     6361 agacctcttc cactagatca ggaacaccaa attccagatc gtcgtatacg attcttccac
     6421 ttgaccgatt tttgctgacc ataaaggctg agttgttggt cacccacaac actgcagctg
     6481 tcttgttctc gattttatct gctagttctt ctcgagtttt cttgatggat ttttgataat
     6541 cggaaatgat tttttctgcc tctttttctt ttgccaaagc tttgccgaca tcttttagtt
     6601 gttcttccca tgtgacatct gttccgtttt ttacaacata agttggcgcg attttgctat
     6661 attgttcgta tttcccgcct tcgacagtag cggaagaaga aatcaacagc aaatcaggct
     6721 caaagcttaa cactttctca taaggaaggt cataagaaat cgttgggaca tcctttaaat
     6781 catcttgcag ataatcttga atggaaccac tgccaacagt ccactgggcg ataggttttt
     6841 cccctagtgc aatcagatag tcttctaagt aactgccgat gatccgtttg ggcgcgtttg
     6901 gaatctctac tttatgtcca agtgtatctg tgactgtatg agtactattg gatgaagtcg
     6961 tcgaattggc agttgtagct gtttctttat ctgaagaggg ggagcaagcg ctcagtactc
     7021 cagccagcag caatatgcta gataatgtga atacttttgt ttttaatttc atttctttcc
     7081 tcctaacata attgagaatc tttctcattg gttattattc ctgatatttc gaacgctgtc
     7141 aaccgcttca tttggctata gatatgaaag tagcgcttga cgaaatgttg aaacagcgtc
     7201 ataattgaga atgattttca gcgaaaggat gggtgaaatg acgagattaa gagcagctga
     7261 cattgcagcc ggctacgaaa acaaaaaaat cattgaaaac atgtctgtta cgattcctga
     7321 agggaaaatc actagtttga ttgggccaaa tggaagtgga aaatctactt tattgaaaag
     7381 ttttacccgt atactacctg tgaaatcagg aaagatcatt gtcaataact ctgatatctc
     7441 tacatacagt tcaaaagaac ttgcaaaaaa aattacatta ttagcacaaa ctagtgaaca
     7501 ccctacaggt atgacagtag aagaagtagt atcttacgga cgataccctt atcaaaaact
     7561 gttcagtggc atggacacgc aagatcatga agcaatcgag tgggcgttgg atgctacagg
     7621 tctagctgga tttcgtatgc gggatatcgc aacgctgtct ggtggacaaa cccagcgtgt
     7681 ctggatcgct atggcgcttg ctcaggaaac agatatcttg atcttggatg aaccgacaac
     7741 atttttagat ccggcacacc aattagagat cctacagtta ttgactgaaa tcaatcaaat
     7801 gaaacaaaca acgatcttga tgtcgattca tgacattaat catgcttcac gtttttcaga
     7861 ttatttatta gcaatgaaaa acggtaagtt gatcacagaa ggtacacctg aagaagtgat
     7921 cacaaaagag tgtatgaaaa aaatatttga gattgatgca acgattgtca cgcttccaag
     7981 aacaaataaa ccagtttttc taacctatga tttgttgcca aaagagcagg aggatcaatt
     8041 atgaggacta tgaagaaaaa tagactcctg cttttggtct gttttgttct tttgattatt
     8101 ttttttgttg ctctttcaaa tggcgcagcg actgtgggaa aagcagattt ttggcaggcg
     8161 ttgtttgatt ttgatagatc aaaacaaagt caccaaatca tacgaaatat tcgtttgcct
     8221 cgtgtggcgg gagcatttct tgtagggagt tgttttgcat tgagcggtgc attgatgcaa
     8281 ggagtgacaa gaaatccttt agcagattca ggcttactag gaatcaacgc gggtgcttcg
     8341 ttggcaatgg cccttagttt tgctttttgg cctaaagctt ctgatttttc tgtgtttcta
     8401 atgtcattgg caggttcttt tgctgtaact ttgctcgtat ttggtttttt gcagttttcg
     8461 aaaatgggca tgaatccttt acagctgatt ttagctggcg tagctattag ttcttttttt
     8521 actgctatca gccaagcact tacccaattc tttaatctgc agcaggatct tgctttttgg
     8581 tttgtaggcg gagctgccaa tatcacatgg aaacaattag cagtattgtt tcctttatat
     8641 atacttgctg ttttggtttc tttttcatta ggaaaagcaa tatctttgat tcatttgagt
     8701 gaaacacatg cattagctct tggaagtcat cctaaacgaa cgcggttgat tgttttctgt
     8761 ctagttgcag tgttagcagc cattgccgtt tcgttagtcg gacctgtttc atttattggg
     8821 ctgttggttc cgccagttgt tcgcggaatg gttggaacgg actatcgtta tgttctacct
     8881 gtctcttttt tagttggcgg tggattggtt atgttggcgg attttacagc gaggatgatc
     8941 aatccccctt ttgaaacacc ttttggatta gtcattgcat tgattggcgt tccgttttta
     9001 ctgatacaag taaggaggga acaacggtga aaaaaatata ttggacgttc tatttcttgc
     9061 tttttgtact tgtcatcctg tctataggta gtttaatgat tggtacgcct tttatttacc
     9121 tggatcagct ttttgccacg ttcataggga aagaatcatc ttctttggcg ctgattgtta
     9181 gagaatttcg agttcctcga ttactgatca gtttattagc aggagcttgt cttggcgttt
     9241 ctggttatat cttgcaagga gtaacaagaa atgaattggc agattctagt atattgggca
     9301 ttaatgccgg agcaggattt ttagtgatgc tttatttagg tttcttttcg cactattcgc
     9361 tgccgtttct attgctagtt gtagcctttc taggtggtgt tttagctgct tgctgtgtct
     9421 atcttgcggc ctatgatcga aatggctttt tgggtatgaa tcgaatctta ttgtccggta
     9481 ttgccgttaa tgccggcctt tctgcattaa cattgctatg cacgatccaa ttatctaagg
     9541 aaaattatgg atttgtgaat gcatggttag cgggatcaat ctggggtgcg agctggcctt
     9601 atgtttgggc attgcttcca tgggcggtta tcttaattcc gctaggagga ctttatgcac
     9661 agaggattgg attgctatca tttggtcaag aacgcgcgca aggattaggc cttaacgtgg
     9721 ctaaaagtca aaaaatcctt ttgttgttag ctgttgcttt ggcaagcggt agtgtagcgg
     9781 tgacaggtag tttatctttt gtaggactac tagctcctca tgctgcgaag ctagtcgtac
     9841 gtaaagaaaa taactggacc tttgttctga gcggtttgtt cggttccatt tttctgctta
     9901 gtgcagatat ggttgctaga gtgattctac caagtgggga agtacctaca ggaattttga
     9961 ttgccctttt tggtgcgcca tattttctgt atgcactttt tatcagacaa aaacacgcgc
    10021 tgaacagttg aaatttatta aaaaaggaag gccaatcatt tctaggtctt ctttttattc
    10081 tatttatgca tttatttgaa agttcgattg ctatttttct ttttttccta taccatttta
    10141 tgagagaagg aagggtgagt ttttggacca attgaaagta agattacgcc ggaaaggaat
    10201 cgaaaagctg aaaaatctgg ccaaaagtgg caagaaaaag gaaaaagaac agcgaatcta
    10261 taaacagcta ttcgccagcg agtactggaa gtcagcaaaa gtgataggga cgactttatc
    10321 gaatgaattt gaactggata cacagccggt tatccaacga gcagaaaaag aacaaaagaa
    10381 aatagttgta cctaaaactt tgccgaaacg tcagatggcg ttttatgaaa tggatgaaca
    10441 gtccgttctt gtacgttcga gttttggtgt tcttgagcca caatcagaga agctctattt
    10501 gccggagatg atcgatctcc tcattgtacc aggtattatc tacaatagag ccggttatcg
    10561 aatcggtttt ggcggtggtt attacgaccg gtatttaaat aattttcata aaaaaacatg
    10621 tagccttgtt tttcgtgaac aattgaatga acaatggcaa ccggagagct ttgatcagaa
    10681 gatccaacaa ttgtttattg actaaaatga aatgggaggt tttcatgaat tatcaacagc
    10741 aaataaaatt gcgtgcatgg ttaagacgac cgttatggac ctatgctttt ttagggatac
    10801 agacagcggt cttcctcatg atggaattat ttccgcgact tgagatccct tattatgccg
    10861 gaatgtatgg tccttatctt gttcacttca atgaatggtg gcgtttagtg acaccaatct
    10921 tcatccattt tggactgatg catttcgtaa tgaactcact gatactgtat tttatggggc
    10981 agcaaatcga ggcaatctat ggccattggc gttttttctt gatttatatg ttcagcggga
    11041 taatgggaaa tacagcaagc tttgctttta acgaagcgaa tgttctgtct ggtggtgcga
    11101 gtacttcgat attcggtttg ttcggtgcgt tgtttatctt agggtttcat tttaaacata
    11161 acccagcgat ccagcagcta gtcagacatt ttcttttatt tattgtgttg acatttgttt
    11221 ttggtcttct ggatacctct gtagatgttt ggggacatgt cggtgggcta attggcggat
    11281 tagtgttagg gaatgttttg ggtctaccga aacaacagac aagctattcg atacaccaaa
    11341 ggattctttc tacactagtt tttgtatttc tttttgtaat atgtatttta ctgggtttaa
    11401 aaaaatatgg tttacttgta taatatgaag gaagtgctta aagatggaga gcttatatga
    11461 tgtacagcag ctgttcaaaa aatttggcat ctttatctat gtgggtgcac gcatctatga
    11521 cattgaatta atgactatcg aattgcgtaa tctgtatgat agccgtttga ttgatcatga
    11581 tacatacatg acagcgtggc gcatattaaa aagagagcac cgaattgaag aaagccgaaa
    11641 gaaaggaaat ttgtaatgga caagaagatt attgggattg atttaggtgg aacaacagca
    11701 aaatttgcaa ttttaacacc agaaggagag attcaacaaa aatggagcat cgataccaat
    11761 attttggatg atggtaagca tatcgttcct gaaatcattg aatcaatcaa ccatcgtttg
    11821 aatttatata atatgaaagc cgaagacttt atcggaatcg gcatgggtac cccaggaagc
    11881 gtggacagcg aagctggaac ggttattggt gcgtataact taaactggac agaagttcaa
    11941 tttgtgaaaa aactgatcga ggcaggaaca ggaatcaaat tcgctatcga taatgatgcg
    12001 aatgttgctg ctttaggtga acgatggaaa ggtgctggag aaaacgatcc agacgttgta
    12061 ttcgttactc taggaacagg tgttggcggt ggtatcgttg ctggcggaaa tttgattcac
    12121 ggcgtgagcg gagcaggcgg agagatcggc catatcactg tggatcctga agggttcgaa
    12181 tgtacttgcg gtaaacgcgg atgtttagaa actgtgtcta gcgctactgg tgtggtacgt
    12241 ctaggtcgtt acttggcaga agaatacgca ggagattcaa aactgaaagc aatgttagac
    12301 aacggtgaag aagtaacaag taaagatatc tttgaaatgg ctcaagaaga cgatccgttt
    12361 gcattgatgg ttgttgatcg cgtatgcttc tacttaggtt tagcttgcgc caacttgggg
    12421 aatacattga atccgtcaag tattgtgtta ggtggaggag tatcggcagc aggagaattt
    12481 ttacgtagcc gtgtagagaa atattttaaa gaatttactt tcccgcaagt aagagaatca
    12541 acaaaaatca aattagcaga gcttggtaat gaagctggtg tcattggtgc tgcctcatta
    12601 gccttgaaat ttgcttaaaa aaacggaggc attacatggt tttatgggta attaatatca
    12661 ttttgctatt gattgtgctt gcaatcatct tttgggaagt ctatctacga gtaatggcca
    12721 agcgttcagc aacgactttg acagaagaag aatttcgaga aggaatgaga aaagcacaag
    12781 taatcgatgt tcgcgaaaaa gatgtgtttg atgctgggca tattttaggt gcaagaaaca
    12841 tcccttacac tgttttgaaa gattcattag gatctattcg aaaagaccag cctgtctata
    12901 tttacgatca aaagaaaagc ttgagcatcc gtacagcaaa taaattacgt aaagcaggtt
    12961 atcaagagat ttatttactt aagggtggat atgacggatg gtctggaaaa atcaaaagta
    13021 aaaaagtata aaacagatga ttgtatgaca gctttaaaaa ccgaatcaat cattagattt
    13081 tgaccaagat cacatgaacg gcgcaatgga tagttgtgcc gttcattttt tttattttgt
    13141 gctattatct acatatataa gctaagcgaa aaggcagaaa gaaggaaaca actatgatca
    13201 aattaaaacg tgtttacctt gatgctgaaa aagaggatgg ctatagaata ctcgtggata
    13261 ggttctggcc acgaggaatt tcaaaagaaa aagcgcagtt agatctgtgg ttgaaagaaa
    13321 ttgcacctag taatgaagca cgcaaaactt ttgaccatgt accagaacga tttgcttcat
    13381 ttgcaaagaa ataccagcaa gaattgattt tcgaagaaga aaagaagcaa gctattttta
    13441 aactcagtca acttatgaaa gaacatccag tcattacttt tgtttatggt gcaaaaaatg
    13501 aacatcagaa ccatgctgtt ttattgaaaa aatttatcga aatggaggca tgcaaatgaa
    13561 aatagggatc attggtgccg gacctagagg attaagtata gcagaaagga ttctccataa
    13621 taatcaggac agacattctc ttgaactttt tcttttcgat cccaatggtc ctggaggtcg
    13681 agtttggcgg ttgaatcagc caccggagtt attaatgaat tctgtttctc aacaagtgac
    13741 gctttttaca gatgaaacat tgtccagtgg aggattggta acacagggac ccaacttgtt
    13801 tcaatggagt aaaggacctg ctacagatta tatcgaacag acggtaacca aaaataagca
    13861 actaattata gaagaaatca atcgtctgga aaaagatcag caatctactc gatgtttata
    13921 cggactctat caacgctggt ttttctcaaa gttgcaagaa gaatttccag cttctattca
    13981 gtttgtttat tctttagtaa aaaaagtcag caaaagaaaa gatggttatc ttttacagac
    14041 agatcagcaa agctattcta tcgatcagct tgttttagca agcggtcact gggaaaatga
    14101 tgagacagaa gaagaaagag tgtgggtaga ctacgcaaag aaaaatgatt tactgtatca
    14161 gcttccttct aatcctgcag atgtcgatct ttcgaagata ccgcctaaag aaaatgtctt
    14221 tttaagaggt ctaggcttaa gtttttttga ttatgtcgga ttgtttacag tatcaagaga
    14281 agggcgcttc gagaacgaaa aaggaaaact tgtctatcat ccatcaggca aggaaccgat
    14341 tgtatacagt ggatcaagaa gggggctccc ttattaccct cgaggaagaa accaaaagca
    14401 aggcggatca atggcatggc cacggttact aacaaaagaa aacctggagc aatggcatca
    14461 gtcgggattt ttgactggcg aaacattttt tgactatctg aaaaaagacg ttgagttgtt
    14521 ttattacaag aaaatcatcg aagaatatca tttgccgatt ccaaagaaaa attttgagca
    14581 tgattttctc tccctctctt cagaggagtg tctcggaaag catccagcac taatccctta
    14641 tagatgggat tggtcgtatt tagaaattcc tcttacctat caggatgagt cctttgcaga
    14701 agctagccga gcatttattg aagggcagat aaaagaagcg gaaaaaggca attgcacagg
    14761 agcatttgct tcaacatttg atgcattaaa agactggcgt gatccaatcc ggcaggctat
    14821 tgaatggcac gttttttctg caaaggaata caaagaaata ctatggggat ggtttactca
    14881 tctaaatagc tttttaacga tcggtccgcc agtcattcgg acgaaagaat tagcagcact
    14941 gatagatgcg ggtatttttc atttgttcga accgccattt gatattcagc aaaagcacaa
    15001 ccgctatgtt atttctctac aagataaaga aatcagcagt cgctatttga ttgaagctcg
    15061 attgccacaa acaagctttt ctcgttcaag taatcctgtc ataaaaaatt tgaaggaaaa
    15121 caaacttgtt cgcccttttt cattttcaga taatacagga agttatcatt cagaagctat
    15181 agatgttgag cgaggtacga atcgcgtgat tgatgcttca ggggaaatcc agtctgactt
    15241 ttattgctat ggaatacctg ttgaaggtgt ggattggcta actgcagcag tagctagacc
    15301 ttatacagat ccatggaatc tgagacaggc agataaaata gcacaactta tattgactga
    15361 caaataaaaa tacgcatgat gacttatctg ttctttcaca agtcttcatg cgtgttttta
    15421 aataattatt aacgagaaac gcgtttgcgc attgcttttt tacggttttc ttcgatcatt
    15481 gcttcttttt cgtcaattgg atcgatcact gtttttttca cagaagcgac caaaccagca
    15541 acggcagcgg ctgttgccag acttcctact aatattccag atacaaattt tttcattgaa
    15601 tccacctcat ttattatttg ttatatctat tatggaacaa aaga
//
LOCUS       DAYYME010000062        15191 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000062 DAYYME010000000
VERSION     DAYYME010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.063"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(399..964)
                     /locus_tag="ACHBFW_001628"
                     /pseudo
     CDS             complement(399..964)
                     /locus_tag="ACHBFW_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1143..1799)
                     /locus_tag="ACHBFW_001629"
     CDS             complement(1143..1799)
                     /locus_tag="ACHBFW_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317935.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(2367..3302)
                     /gene="whiA"
                     /locus_tag="ACHBFW_001630"
     CDS             complement(2367..3302)
                     /gene="whiA"
                     /locus_tag="ACHBFW_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFM9317936.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(3336..4334)
                     /gene="yvcK"
                     /locus_tag="ACHBFW_001631"
     CDS             complement(3336..4334)
                     /gene="yvcK"
                     /locus_tag="ACHBFW_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFM9317937.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(4331..5215)
                     /gene="rapZ"
                     /locus_tag="ACHBFW_001632"
     CDS             complement(4331..5215)
                     /gene="rapZ"
                     /locus_tag="ACHBFW_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFM9317938.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(5350..6081)
                     /locus_tag="ACHBFW_001633"
     CDS             complement(5350..6081)
                     /locus_tag="ACHBFW_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFM9317939.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(6085..7350)
                     /locus_tag="ACHBFW_001634"
     CDS             complement(6085..7350)
                     /locus_tag="ACHBFW_001634"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFM9317940.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7613..10429)
                     /gene="uvrA"
                     /locus_tag="ACHBFW_001635"
     CDS             complement(7613..10429)
                     /gene="uvrA"
                     /locus_tag="ACHBFW_001635"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFM9317941.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10439..12433)
                     /gene="uvrB"
                     /locus_tag="ACHBFW_001636"
     CDS             complement(10439..12433)
                     /gene="uvrB"
                     /locus_tag="ACHBFW_001636"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFM9317942.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            12692..14194
                     /locus_tag="ACHBFW_001637"
     CDS             12692..14194
                     /locus_tag="ACHBFW_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM9317943.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            14196..14933
                     /locus_tag="ACHBFW_001638"
     CDS             14196..14933
                     /locus_tag="ACHBFW_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9317944.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
BASE COUNT         4400 a         3291 c         2558 g         4942 t
ORIGIN      
        1 ttatgtacgg tgattcacac tagtatccca atcacttttt aagttatccc gatttcaatt
       61 ctacttcaaa aatagttgct tttatgtatg ctgttagttg attagatggt cgtttgttga
      121 gctaggtcgt ttattttaaa caagttgttt ctttagctta tacctatctc gtctaccaaa
      181 cgcaagatgg caaacacaaa tgcatacgca tctgtatttg ccactttgat tcgtataaag
      241 ctaacaactc ctaactcctc tcatttaata gttgctctct tttttattca gatacatttt
      301 tgctgatcac agcctagtat ttcaatggtt ttgaacacta aaccaaattg ttccattaca
      361 acattcactg gctgatgaaa tggattaata gatttcgatt attcaaagcg attttctatt
      421 ggtggattgt gttcccaact aacaagacgg attccaaagt cattatataa ctcttcaaga
      481 gttaaccctt tcctgttccc aatagatgta tctctcccac gctcgatata aaatatggag
      541 tttcgaatgc caagacctgg caaatctttc cttgttacag ttacactgct actaacttcc
      601 ccatataaaa tccagctcct ccaaaatgat aatatatttt tcgaccatca tctaactcat
      661 gtaacaataa ttgttgtgcc ctcttgtcac tcgccaaatg caaacgccac gtgcgatacc
      721 atgccatgct acctgcatct gcagaaaatc catcaaataa tgcaacaatt gtatcttcta
      781 tcacaatcct ttccccatct acttcatatt ctaatctaaa atcaaactcc cctcgggtaa
      841 tcgcaggtct tggtggctta ggacccaata ttattctact taaaaccgca ttcgttccca
      901 gatgaaacac agctataaca acaataatga ttaatatttt gtttctattt gataaatttc
      961 tcattattcg caaatcacct ctacattctc acatactagt tcgtaatact cctttaatat
     1021 caaaatatct actgatcgta ataatattcg ctatgaattc ctactactcg tataagaaag
     1081 gccaaatcat cagcttttac ttgctgatga tttggcttgt ttctatttat cgtaattgtt
     1141 ttctaatcac caaaattgtt aacaatcggt tcagatattt cccaattcac tagccttata
     1201 ttatattgat ttaacaattc ttcttcattt attgtatttt gatcaagcgg accaaattgt
     1261 tctaataatt ctttgtcatg taaaatatcc cttctagtaa tgcctctttc cctttcaatg
     1321 aaagaagcac taggaaaagt atgattataa ccattcaacc tatctcccat gtaataatta
     1381 gcaggtcctg gaaaataaaa tatttgctta ttatcactaa cttctaacag caacactaaa
     1441 tcttctccgc tggctaaacg acttgtccat ctacgatatc tccctatgcc ttcgttcatt
     1501 ccaaatccat caaattcaac tattaacgtg tcttctatca catgtaattc ttcattcaat
     1561 tcatactcaa gtctaaaagg aaattctcca tatgtaatct ctggcaatgg tggtgctgga
     1621 gttctccaaa taaatacaat aactgtgaat agaaaaaatc caacgatcga tataaatgtt
     1681 atgagacctg ctcgaagtaa aacccgatat aataaaaatg ctgtatttcg ctttgctata
     1741 tctagaaaaa taaaaagtac tgcaatcata atgatagtaa aagttgtaaa aaaacgcatt
     1801 tttgccctcc ctttataaac atcgattctc aaattctaaa ccaaatcttg gtagttcaag
     1861 ttcaggtata acgtattcca accatacatc tcaattacta tttatgaact tatgattatc
     1921 actgaataaa tcactccatc cccttgttaa aacttctaca ttaattacac ctggttgttt
     1981 tcgactagct aatccaatga acttatcaac catccttatc caccaaccac gagaatcttg
     2041 accagaagta agaagagcag ttctcaagga tgatcgagca gttccttcca gttttttaat
     2101 taatcaggcc agttatagat aaatacttga tcttatctat atcaggactt ctatattaag
     2161 caaaaattta tgtacaaaac taaaatcttc attcagttgt ctccccgctt tttatacgat
     2221 tgtattaaaa acaatagaag cctttccaaa ttatgttact ccagtaaaga tggaattata
     2281 gtttgacgga agaaaagcaa gtataacttt taaaaaaaac tgatacattc gcatctcctg
     2341 cttcatgtat cagttttttc tttggattaa actgcttctt cacgcagttt ttccgcaaat
     2401 tcatttattt tgcgaattcg gtgattaatt cccgattttg atatcgcacc tgagggaatc
     2461 atttccccta attctttcaa gctgacctct ggatgttgaa gccgaagttc tgcgatttcc
     2521 tgaagttttt caggaagggc atgtagaccg acctttgctt cgatcaattc gatgttttcg
     2581 atttgtttag aagccgcatc aatcgtcttg ttcagatttg ctgtctcaca attgactaga
     2641 cgattgaccg aatttctcat atcccgtacg attcgaacat cttcaaatcg gagcattgag
     2701 ttggttgcac caatcaatgt taagaaatca gcaatttttt ctgctccttt taaataagaa
     2761 atatagccat tgcgccgttc aagcgttcga gcatgtagac cgtaataatt taacatatcg
     2821 caaatgtctt ggttatgctg ttcataaata gaaaatattt ccaaatgata acggctcgtt
     2881 tccggattat tgactgaacc tgaggccata aaagcaccac gtaagtaaga acgcattttt
     2941 tgcgaatttc ccataatctc gtccgaaacg tgtccattga acatcatacc atccatgata
     3001 tcaagatcca tcaacacatc ttttgtgcct tgcttcaacc ggacgatata gacattgttc
     3061 ttttttaatt tcattttttt acgaacgagt agctcacttc gtacctgata atgatctttc
     3121 aaaagagaat agatacgtct ggcaattgca gcattttctg tttggacgtt caaaataaac
     3181 tgctggttca caatacctaa agagccgttc attcgcagca aagcagctaa ttccgctttc
     3241 gcatgttctc gatgaacttc caaaccagtt agttctttct tcacatctgc agcaaatgac
     3301 atcctattct cctcctttct ccaacaataa attgcttaat attttgaacc gaaaactaaa
     3361 cggaacaatt cttctactac tttgtctcca tcgtggaaga caccaccatc tttcaattcc
     3421 aaaaagtcgg tggaaatcac tcgacatcct tcatttctca atccgttgaa gtcgtgtgcg
     3481 acttgtacta gatactcatc atagatctct gggtccatat aatcttcagg cactttttcc
     3541 gtattgacca atactgtatc gataaaaggc tgattcaaat gcttgtgtaa aacacgtata
     3601 tgatcggcat ctgtgaaatg ttccgtttcc cctttttggg tcatgatatt acagatatag
     3661 acggtttcag cagttgtctt gagcattgct tcgccgattt ctggtatcac taaatttgga
     3721 aggatactgg tgaataaact tccgggtccc aaaacgacca tgtcggcgtc cataattgct
     3781 gaaacgactt ttcgagccgc tttggcttca cttttatcat ttgtgttgca gacatacaca
     3841 cggtcaatgg ttttacggtc gatcgcgatt ttagattctc ctactgcagt cgttccgtct
     3901 ttaaagacag catgcaaggt caaaggagtt tctgaagaag gataaacatg tccatctaca
     3961 tgcatcattt ttgctaataa ttgaatcgcc tcataagtgc ttccccgcat ctcggaaact
     4021 gcggcaatga tcagattacc gatcgtatga ttggctaaaa actgatcttc ctttttgaaa
     4081 cggtattgga aaatatcttc atagaattgt ggcatatccg ataacgccac taagacattt
     4141 ctcaagtcac caggaggagc catagcgatc gattctctga ttgcaccgct gcttccccca
     4201 tcatctgcaa cagtcactac tgctgtgata tctgctcctt gatttcgcaa acttttcagg
     4261 ataactggca gaccagtgcc accgccgacc actacgatct ttggttttct tatccggtag
     4321 gttttcattt tcacgaccgg ttcaccgtct ctttcctttt atccttgtcc cgatgtgtca
     4381 cattgacttt atagtttttc cctaaagcat ttgccaaacg ttcagtcaat gccacagaac
     4441 ggtgttgacc gcctgtacag ccgattgcaa ctgtcaagct gctttttcct tctttaatgt
     4501 atccaggcaa tacagtttcc agaagatcca aaaatttatg gtagaatgat tgcgtttcat
     4561 caaaggacat cacataatca tagactggct gatcttttcc ggtcaatggt cttagttccg
     4621 ggatataatg agggttgggc aaaaaacgca catccatcac aatatccgaa tcgatcggca
     4681 acccgtattt aaaaccaaat gatacaaatt cgatccgaaa tccttggtct ccctttgatt
     4741 tgaatgactc attgattttt tcacgtaatt gtcgtggtgt caatgtcgtc gtatcaatga
     4801 tcacggaagc ttgggctttc aagtcgtcta gtatcgcccg ttctttacgt atcccttcag
     4861 tgatcaaacc gtccatcgcc attggatgtg ctcgtcttgt ttctttgtag cgagccacta
     4921 gttcttcgtc tgaagcatct aaaaataaga tagaagtgtc gatgaagttc gtattttcga
     4981 tttcgatcag catgttttgg atttcttcaa agaaggaacg agaacgtaaa tccactccta
     5041 aagccatttt tgtgactttg cctgattctt tgattaattc ccagaatttt ggaatcaatc
     5101 gaggcggcat attatcgata caaaaatagc ccatatcttc aaaactttgg atagctactg
     5161 ttttccctgc accgctcata cctgttatga tgactaattg caaattatct gtcataatta
     5221 cgaactcctc tcacattgtg catagtaaaa ttatatcatt ccgggcgtat cattaccagt
     5281 tttctttttc ttttacaaaa agatttggac caaatatctt ttaggatatt tgccccaaat
     5341 ccaatttttt tacttttccg atgctgtatc caatgccttg cttctttcag ctagcgctcg
     5401 ttcaatcgtc cgatctgcta aaatagattg ggcatcgatc actggataat ggttatcttc
     5461 agcaaattca tgcatcaatg acaattctgt gcaacctaag ataacttttt cacagtttag
     5521 gcgctctacc gcttcttcta agatttcgta atatagctct tgattcaaat aatccgattc
     5581 cttgatctca tgatagatca gataattgat tttttcttga agaacagtat caggaatcac
     5641 tgtttcgaat ccaagatttt tgacttcgcg ttcatagatt ccagctttca tacttccttc
     5701 tgttcctaga atcgctactc tccctgtcgt atgctgacgg acaagttcat ttgctgcttc
     5761 tcttggcata tgaaggatag gaatatccgt tgccgcttgt aattcttcaa aaaagtagtg
     5821 tgcggtgtta caagtcaaca caatgaagtt cggctgcaat aaattttgtt tctcaatatc
     5881 atccagcaga aacggcattg gattttcttc tgatcgatct aaaatataag ctgtacgatc
     5941 aggaactgtt gcatgattga ataagacata attcaaatat tcttgatctt ttgtagcttt
     6001 cgttcggtgg ttgatcaagc gaacaaaact ttctgtcgcc atcgtgccca ttccgcctaa
     6061 aatactgaaa aaattctcca tgaactagcc ttctttcaca tgaaaatact ttttgaatct
     6121 tggaatatag ttatggaaca tatatttcat caacaaccag cgcttgattg accggtcttt
     6181 ttcataaaac acagtagtac cgtaacgatt ttctttgatc atttgctcag ctagtttttt
     6241 gtcttcattt tcccgagcat attctaaaaa gatttttttg ggcacaccca tccataatct
     6301 tgctttatct gattgattcg ttccataggt cgtttcaaca aagggtttgt taaacactcg
     6361 atcttctgtg acgaaacgag ccagattcaa gccgttcaat gtcacaaaga aactgctacg
     6421 accttgtctc aaattgattt caaataattt atattctcca tctctaggat catacttcat
     6481 atcgaaattg gcaaagcctg tatattcgat tttttccaaa aatgccttaa tggtttgata
     6541 gattttttcg ttataatctg gcatgatcac aacgtaatta ccaatcgatg ctggtgtagg
     6601 atcttctaac agtggatgac caagacacat catccgaact tgatgatctt catctacata
     6661 cgcatttagc acacgcatat tactatcatc acccgggata aaatcttgta caatcatttc
     6721 acttttataa ccggcttcgt atatccggcc taaaattaaa tcgaactctt ctctagtctc
     6781 taaaataaaa gctttttttc ttccttcaaa ttggacagaa agatactcca cactgtttgc
     6841 aggtttcaaa gcaactggga attcaaaagg caattcctgt tccaaatgtc catttactaa
     6901 catttcttct ctgacgatca atgttttagg atacggaaga tcatattctt cacaaacttc
     6961 ataaaagctt actttattga tcagtctttc aaataaagaa taatcaatat atggacagat
     7021 aaaatattct gacagctctt gtttatgttg ggagatcaat tctgcatatc catcaccaca
     7081 agcaatcaaa agatattttt tactcttatc agaatatctt tcttttgcca aacgaagcat
     7141 cgtctcgata aacacagggt ctttatcaaa tcctggaata acttctacat tgacaatttt
     7201 actgtagcgg gtaggggcta actgatccga agcatatgct tggcagattt tgccgtatgc
     7261 ttcattaaaa gaacgtgcca taccataaac gttcatgtcg cttcctaata atatagggat
     7321 aaattcttca ttttcaatac tgttcatcat tatctctcac ttactcataa tctcttgttt
     7381 ttcttttcca actacacaag gaatatcata ccataaagaa aaaagcaatc aaaggaaact
     7441 cctccgattg ctcaattctt ttttgaacgg attgaactta taggaagttt ttgtctatac
     7501 caatcagtgt agaaagaact accatgtaat gattctagaa cactgatatt aataaaacaa
     7561 gcaaaaattc atttttttat gtttttttaa ctatctcagc tatctatcct ctttatacca
     7621 ttattttttt caaatagtga cctgtataac tttcttttac gttaatgatt tcttctggtg
     7681 ttccagtcgc aaggatcgtt cctccacctt ctccaccttc tggtcctaaa tcaatgatat
     7741 aatctgctgt tttgattaca tctagattgt gttcaatcac taaaactgtg ttaccagcat
     7801 ctactaatct ttgtaataca tgcaacaagc gggcgatgtc atcgctatga agtcctgtcg
     7861 ttggttcatc tagtatatag aaattctttc cattagagat tttgtgcaat tcactggcaa
     7921 gtttcatccg ttgtgcctca ccaccggaca atgtcgttgc tggttgcccc atcgtcacat
     7981 aacctaagcc aacatcaaca atcgtttgca gtttgcgatg aatctttgga atgtgcttga
     8041 agaattctac agcatcttct actgtcattt ccaaaatatc agcaatgctt tttcctttgt
     8101 aatgcacttc taatgtttca gagttatatc gtttgccatg acatacttca caaggaacat
     8161 agacatcagg caaaaagtgc atttcgatct taataattcc atccccgcga caagcttcac
     8221 aacgaccgcc ttttacgttg aaactaaaac gccctttctt ataaccccgc atttttgcct
     8281 cgttcgtttg agcaaataac ccacggatat catcaaatac acttgtataa gtcgctggat
     8341 tacttctcgg cgtccggccg attgggcttt gatcgatatc gatgatcttt tcgatacttt
     8401 cgtagccgga aattgtcttg aatttacctg gcttagcaga attcttattt aatttttgcg
     8461 ctaacgattt ctttaagata ctattgacca atgtactctt tccagaacca gacacacctg
     8521 tgactgctac aaattctcct aatggaaatt caacactaat atttttcaga ttgttttctg
     8581 aagcaccagt gatcttgatt gctttcccat tacctttacg gcgttctttt ggtacaggga
     8641 ttgatttttt ccctgaaaga tatttccctg tcaaagaatt ttcatcatta gctacttctt
     8701 caggagtgcc tgcagccacg atttgtccgc cttgttctcc ggccccaggt ccgacgtcaa
     8761 tcagataatc tgctgcccac atcgtatctt catcatgttc gactacaacg agggtgttgc
     8821 caagatcacg cattttcttt aatgagccaa tcagtcgatc gttgtcgcgt tgatgcaatc
     8881 cgatcgatgg ttcatctaaa atatacaaaa ctccagaaag attcgatcca atttgtgtag
     8941 ccaatctgat tcgctgcgct tctcccccag ataatgttcc ggaagcgcga ctcaaagtta
     9001 gatagtctag tcccacattt tccaagaatg aaagtctgtc atttacttct tttaaaatcg
     9061 gtttggcaat tactttctct tgttcagata acgttaagtt tgagaagaaa tctactgctt
     9121 tttgaataga caactcattt gtttgcccga tattcgttcc attgatttga acagatagag
     9181 cttggggatt caatcgataa cctttacatg tctgacaagt taattctgtc atataaagac
     9241 gcatttgctc acgcgtgaaa tcactgttcg tatcatgata acgacgctcg atattcttca
     9301 ataccccttc gaaagtcgtt tctacatcgc gaacgccacc aaaatcattt tcataatgga
     9361 aatggaaaag atcatctgtc ccattcaaga tgatatcctg atgctcttcc ggtaattctt
     9421 cgaatggtgt atctaaatcg ataccgaaac tttcacaagc ctgcgctaac atttgaggat
     9481 aatactgaga gctgattgga ttccatggaa cgatagcacc ttgactaagt gtcttcgttc
     9541 tatctggaat cactaaatct tgatcgactt ctaatttgac acctaaacca tcacagtctg
     9601 gacaagctcc aaaaggcgca ttgaatgaga acagtcttgg ttctagttcg ccaacagtaa
     9661 atccgcaata aggacaggca tagtgttcgc taaacaacat ttcttcttca ccgatcacgt
     9721 caaccagagc atagccgtct gccaatcgca gagccgcctc taaagaatca aatagacgag
     9781 aacgaatccc ttctttcaca atgatccggt cgatgacgat cgcgatatca tgttttttgt
     9841 ttttctccag ctcaggcact tcacttaaat catagatctc accatcgact cgaacgcgga
     9901 cgtatccctc tttttggatt cgttcgaata tttttttatg ttgtcctttt ttctttacca
     9961 cgatcggtgc caacacttgg atctttgtgc gctcaggcaa agccattact tgatctgcca
    10021 tttgatctac tgattgactg gtaatctcga tatgatcgtt gggacaaatc ggatgtccta
    10081 cacgtgcaaa caacaagcgt aaatagtcat tgatttccgt cacagtacct actgtcgacc
    10141 gaggattttt acttgttgtt ttctgatcaa tagaaatcgc cggactcaat ccgtcgatgc
    10201 tatctacgtc cggcttatcc atttgtccta aaaactgacg agcataagca gaaaggctct
    10261 ctacgtatct ccgctgccct tcagcgtaca gcgtatcaaa tgctagggaa ctttttccag
    10321 aaccggacaa acctgtaacg accacgaatt tatctcgtgg aatcgttaca tctatatttt
    10381 tcaagttatg ggcacgtgca ccgtgaatcg taattttatc gttagccatg ttgtcctcct
    10441 actttgaagc ttttaattct aaaatcatat ctcgcacatt agcggctgtt tcaaaatcca
    10501 atgctttcgc cgcatttttc atttcttttt ctaatttcat caataacgta tctttttctt
    10561 ctttcgtcat ttcttcatac gagacttgta ccgcttcttt tgtatcatct tccgattctt
    10621 tcgtaataga aatcaaatct cggatttctt taatgatcgt ttttggaacg atcccatgtt
    10681 cttcattgta cttctgctga atcgtacggc gtcttgatgt ttcatccatc gctagacgca
    10741 tagaatctgt tactttgtct gcgtacatga ttactttacc ttcttcattc cgagcagctc
    10801 gtccaatcgt ttggactaag gaacgttcac tacgtaaaaa gccttcttta tccgcatcca
    10861 agatagcaac aagggaaact tcaggtacgt ctaatccttc acgaagcaaa ttgattccaa
    10921 ctaacacatc aaattcacct aatcgaagat ctcgaatgat ttctgttcgt tccagcgttt
    10981 tgatatcact atgcaaatat ttgactttga tccctaattc tttgaaataa tctgtgaggt
    11041 cttctgccat ttttttcgtc aatgtcgtta caaacacccg ctggtctttt tcgactcgtt
    11101 catttatttc gcctactaga tcatcgattt gtcccatgat tggacgaact tcgatcactg
    11161 ggtcaagcag ccctgtcgga cggatgattt gttggataac ggtatctgtt tgttcatgtt
    11221 catatggtcc tggcgtcgca gagacataaa tgatctgatt gacatgtttt tcaaactctt
    11281 ctaaccgaag tggccgattg tctaaagcag atggcaaacg gaaaccgtaa tctactagca
    11341 tctgttttct cgctcggtca ccattataca ttcctctgac ttgaggcatg gttacgtgcg
    11401 attcgtccgc cacgatcaaa aaatcttcag gaaagaaatc caatagcgta taaggcggtt
    11461 caccttcttt acgtccgtcc atatgccgag aataattttc aattcccgac gtgtagccca
    11521 tttctaacat catttcaata tcgtaattgg ttcgctgttc taaacgttga gcttccagaa
    11581 gtttgttttc atttcgcaag acagttaaac gctgttctaa ttccgctttg atattcgcta
    11641 cggcatgctc catatgttca tcattcgtca caaagtgagt cgccgggaag attgccacgt
    11701 gttccgtttc tcctaaaact tcacctgtca gtgcattgac ttctcggata cgttcgacct
    11761 catctccaaa aaactccaca cgtaaggcac gttcatctct tgaagccgga aaaatctcca
    11821 cgacatcacc gcgcactcga aaacgaccgc gctgaaaatc tatatcattt ctttcgaatt
    11881 ggatactgac taagtcacga atcaactgat tgcgatctag ttctgctccc tgtcgaatcg
    11941 aaactacttg tttttggtac tcaaaaggcg aacctaatcc aaaaatacat gaaacggatg
    12001 caatgacgat cacatcattt ctttctaata atgaactagt agccgaatgt cgtaatttgt
    12061 cgatctcatc atttacactt gaatcttttt cgatatacgt atcgcttgaa ggcacataag
    12121 cttcaggctg ataataatca taataagaaa caaaatattc aacagcattg ttagggaaaa
    12181 attccttgaa ttccccgtac agctgaccag ccaatgtttt gttatgggca ataattagtg
    12241 ttggtttatt cactttctca ataagattgg aaatcgtata tgttttccct gttccagtcg
    12301 cacctaataa gatttgtgct tttttcccgc cgactacgcc atccactaat tggttaatcg
    12361 cttcaggctg atcaccagcc ggttggtatt tcgagacaag ctcaaagtgt cgggaggtat
    12421 ctctctcaat cattcttcgt cctccttttc attcattcct tttcatttta cgaccaataa
    12481 aaagaaaagg aaagcaaagg cattccttac taaaaaatta taccacaccg aacatacttt
    12541 cgccactttt ttatcttatt atgcatgtta cgttttataa cataaaatgt tagaaactcg
    12601 catttttttc attttttcga gcttacagtc ttgtctgatt cagcactttt cgcctataat
    12661 aataaaatat tggaaattta ggaggttgat tatgaaaaga aagcatttat ttcactcatt
    12721 tgttttctta atgacaggac ttgtgacact tgcgtttgga catgttgcac aagctgacaa
    12781 cactacaacg gcagagtcaa gcacaagcga gacaatgcaa aaaatagagc ccaaaaaaga
    12841 cgtatacgta attgccagtg actctgcttt tgctccattt gaatttcaaa acgcaaaggg
    12901 cgagtacgaa ggcatcgatg taaaattgat ggaaaacatc gccaaactgc aaggatttaa
    12961 catccgaatg gactaccgtg gtttctctgc tgctttgcaa gctttggaat caggacaagc
    13021 agacggaatg attgccggaa tgactgtaac agatgaacga aagaaaagct atgatttttc
    13081 tgattcttac tttgatagcg ggatccaatt agctgtcaaa aaaggagata cttcgatcaa
    13141 gtcttatgaa gatttgaaag gaaaaacagt cggagcaaaa gttggtaccg aaagtgctga
    13201 atttttaagc gaccatgaat ctgaatatgg ctattctatc aaatattttg atgctgctga
    13261 tcaattgtac gacgggttaa aagttggcgc aatcgatgca atgatggatg attatccggt
    13321 aatcggttat gcgatcaaac aaggacaaga attggaaaca ccgatcaaaa aagaatctgg
    13381 cggaaaatat gcatttgccg ttaaaaaagg acaaaatcct gagcttctta atatgttcaa
    13441 tgaaggattg aaagaattaa aaagaacagg cgaatacgat aagattatta aagaatatgt
    13501 agcggatggt tcagaaactt ccagtgagtc ttccactgct gatgaatcaa catttcttgg
    13561 acttatccaa aacaactatc aagcattgtt gaaaggactt tggagaacaa ttctcctaac
    13621 tttgatctcc tttgctttgg ctatagtaat cggtgtgatt tttggattat tcagtgtagc
    13681 accaatacgc gctcttcgca caattgctag tatttatgtc gatatcatcc gaggaattcc
    13741 aatgatggtt cttgcgttct ttattttctt tgggttgcct ggtatcgttg gatttacgat
    13801 tcctgacttc cttgctggtg ttatcacctt gactcttaac gcaagtgctt atatcgcaga
    13861 gattgttcga ggcgggatca acgccgttcc tgtcggacaa atggaagctt ccagaagttt
    13921 aggtctttct tataatcgga caatgcaaaa aatcattttg ccgcaagcga tacgcatcat
    13981 gatcccatca ttcgtcaatc agttcgttat ctcattaaaa gacacaacga tcatttccgc
    14041 aatcggtgta gtcgaattgc tgcaaactgg taagatcatt gttgcacgta caacacaaag
    14101 tacgtatgtt tacttgatca ttgcgatcat gtacttgatt ttgatcacag cattgacaaa
    14161 attagcaaaa gttttagaaa agaaggtgaa gtaagatggc agaaaaaatc ttagttgagc
    14221 atttagtcaa aaaatacggc gataacacag tattaaacga catcaatgtg tccatcaacg
    14281 aaggagatgt cgtctgtatc attggccctt caggttcagg aaaaagtaca tttttgcgtt
    14341 gcttgaatcg tttagaagaa ccttctgacg gtgaaattat tattgacggc gcccacttga
    14401 tggataaaag cacagatatc aacttggtga gacaacatat cggaatggta ttccagcact
    14461 ttaacctatt ccctcatctt tctattttgg aaaacatcat tttagcaccg atggacctga
    14521 agaaagaatc tagagaagaa gcagagaaaa aagcaatcaa attgctgcag acagttggtc
    14581 tagaagataa aaaagatatg tatcctgaaa tgctttccgg tggacaaaag cagcgtgtgg
    14641 ctattgctcg tgctctagcg atgaaccctg acatcatgct ttttgatgag cctacatctg
    14701 cactcgaccc cgaaatggtc ggtgatgtct tgaacgtcat gaaaaagtta gcagaacaag
    14761 gtatgacgat ggttatcgtt acgcacgaaa tgggcttcgc taaagaagtg gccaatcgtg
    14821 tcatgttcat tgacgacgga aactttttag aagacggaac gcctcaacaa atctttgaaa
    14881 atccgcaaaa tgaacggaca aaagatttct tgaataaagt attaaatatt taataaaaac
    14941 caaaaatgcc cggtcgcttc atgacgaccg ggcattttta gtttcttgtt ttttcactta
    15001 actgtaccag ccgcatcgct tgttctcctg cacaaacaag caatcgctct acctgttcca
    15061 tcgcataaga aagtaccatc ggttcattga cgatatcaag tacatagtcg ataccacctt
    15121 cagaaactat gatgattatc ttattctaaa gctggctttt ttgttggtac aaacatgaca
    15181 actacaaaaa a
//
LOCUS       DAYYME010000063        14655 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000063 DAYYME010000000
VERSION     DAYYME010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.064"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            459..791
                     /locus_tag="ACHBFW_001639"
     CDS             459..791
                     /locus_tag="ACHBFW_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9317945.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            701..895
                     /locus_tag="ACHBFW_001640"
     CDS             701..895
                     /locus_tag="ACHBFW_001640"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9317946.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            934..1080
                     /locus_tag="ACHBFW_001641"
     CDS             934..1080
                     /locus_tag="ACHBFW_001641"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317947.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            1518..2858
                     /locus_tag="ACHBFW_001642"
                     /pseudo
     CDS             1518..2858
                     /locus_tag="ACHBFW_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3099..4715)
                     /locus_tag="ACHBFW_001643"
     CDS             complement(3099..4715)
                     /locus_tag="ACHBFW_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM9317948.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            4833..5051
                     /locus_tag="ACHBFW_001644"
     CDS             4833..5051
                     /locus_tag="ACHBFW_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9317949.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5246..5707
                     /locus_tag="ACHBFW_001645"
     CDS             5246..5707
                     /locus_tag="ACHBFW_001645"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9317950.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5900..6139)
                     /locus_tag="ACHBFW_001646"
     CDS             complement(5900..6139)
                     /locus_tag="ACHBFW_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9317951.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(6163..7686)
                     /locus_tag="ACHBFW_001647"
     CDS             complement(6163..7686)
                     /locus_tag="ACHBFW_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM9317952.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(7704..7826)
                     /locus_tag="ACHBFW_001648"
     CDS             complement(7704..7826)
                     /locus_tag="ACHBFW_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM9317953.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(7856..8839)
                     /locus_tag="ACHBFW_001649"
     CDS             complement(7856..8839)
                     /locus_tag="ACHBFW_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM9317954.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(8863..9012)
                     /gene="rpmG"
                     /locus_tag="ACHBFW_001650"
     CDS             complement(8863..9012)
                     /gene="rpmG"
                     /locus_tag="ACHBFW_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM9317955.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(9033..9410)
                     /locus_tag="ACHBFW_001651"
     CDS             complement(9033..9410)
                     /locus_tag="ACHBFW_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317956.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(9442..9621)
                     /gene="rpmF"
                     /locus_tag="ACHBFW_001652"
     CDS             complement(9442..9621)
                     /gene="rpmF"
                     /locus_tag="ACHBFW_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM9317957.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(9636..9905)
                     /gene="rpsN"
                     /locus_tag="ACHBFW_001653"
     CDS             complement(9636..9905)
                     /gene="rpsN"
                     /locus_tag="ACHBFW_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM9317958.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(10428..12194)
                     /locus_tag="ACHBFW_001654"
     CDS             complement(10428..12194)
                     /locus_tag="ACHBFW_001654"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9317959.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(12154..13908)
                     /locus_tag="ACHBFW_001655"
     CDS             complement(12154..13908)
                     /locus_tag="ACHBFW_001655"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9317960.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(14018..14587)
                     /locus_tag="ACHBFW_001656"
     CDS             complement(14018..14587)
                     /locus_tag="ACHBFW_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFM9317961.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         4684 a         2845 c         2228 g         4898 t
ORIGIN      
        1 taaatttgaa actatcttct ttataattat aaagtatcga ttttacagcg ttctataatg
       61 attcatagaa tctaaaacga atgattttaa ttatcaacgt tttttaattc gtttttagct
      121 taaaaataac ttttgtcaca gtctcactcc taaaattact ttatttacgt atgaagtggg
      181 caggaggtcg aatccagaac tgggctatgg tacttcaacg aggacttctc ctctagaatt
      241 gaaaagcatg cgatttacct tccgtaacga cggtagattt attcaactat gtgtccaatc
      301 aaaatttgtc tgatttcaaa aatagtcaag agaattttgg ttgttaaaaa ctcttatcta
      361 tcaacttcca cagtttagtt gacaaaccct gatactaagt tgggctatga tacacttttc
      421 ttgactatca gttacgttac cggaaggaat gaactctatt gagaaaaaca tatgatcgta
      481 aatttaaact aaagatttca caggatatcc ttgagaaaaa agtaactacc aagaaaattg
      541 ctgaagaata caatatctct cgtccaacaa tttcccgatg ggtttcagaa tatcgtcgat
      601 acgggaaaaa tgcttttgct ggacaaggaa aaagattacc tgataaagca gatttctaca
      661 ttcttgaaca ggaaaataaa cgactaaaag aggaagacga gtgttctacg aacacaaaga
      721 tcgatacgga tctccaggga tcactcaaaa actttacgat gaaggaatcg aaatcaataa
      781 acgtgttgta gctgttctta tgcgtgaaat gaatttatgc acaagaggat ttcatcgacg
      841 caaatcttct catggtagag aaaaaacaat caaagaaatg gtaaaaaaat gttgaatcga
      901 taatttgaac agaaccaaat tgatgctgtt tgggtgatgg atattactta cattccatgt
      961 aatgacggtc gcctctattt gtccacctat attgacttag caactcgtat tctacgttac
     1021 tatatggtag attcacacat gaaaaaagag attgtcgtcc gtccattaca aatttattaa
     1081 ggagaattgc ctacggtaat tcattccgat agaggtagcc agcatcggtc ttttatttat
     1141 caagagctgt tgaacgacca ccacatcaca cattcagtga gccaaactgg gactcctgtt
     1201 gacaatgcag taattgaagc gtttcatcga tcaatcaaat gagaattaat cgactccaac
     1261 aaatataaaa gtaaaataga gatgaaggtg ttaatccaag attatttaga agactactac
     1321 ccaaacaaaa gaatccatac aaaatttatg atgactcctt gacaatttga agcgaagaaa
     1381 ataagggatc tataatatct agtgttggtg acgccttctg ttgccaatac tttttctttt
     1441 ttctaataaa cgcaaacaga caccttaaaa tcctttagaa taaagtcgac gaaaaccatt
     1501 caaaggagtg tttcaagatg tcctattcag agtttaccaa agagttacta gatattctag
     1561 acctaaatct tatctttcat gaagatgcct ttcgcaaaga gcgaatcaat gacgaaacat
     1621 gttttgtttt tgatggcaca ttgacctatc aaccggagga atgctttcat cgccactatc
     1681 aaaatgaaca aacgatcatt aaatagggct ggaaaaaagt atccatctta ttgaatgatg
     1741 tcagtaacta caagaccatt ctgcggatta acaaacagcg cttcaaatgt aaacactgtg
     1801 gaaagacttt tttagcagaa gattcagtta gtgatcgtcg ctgctccatt gctcgcagag
     1861 tcaaacaagc cattcttgag ctattaagcg aacctctttc tatgtctttg atcgcacgta
     1921 tgaagcacat ttctccgacc actgtcattc gaatacttcg cagccttcgg cctaaaacca
     1981 tttctttgag ccagctgtta ccagaagtgg tgtgtttcga tgaattcaaa tcagtgaaaa
     2041 atgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
     2101 ttgtggaaaa tcggcaactc aatcctttac gcgactattt cttgcgctac cctataaaag
     2161 tccgtgaaca agtgcgtcta gtggtttcag atttctatac accctatcgc acattggtga
     2221 aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacattg
     2281 gtcgggcttt taccaaccac cgaattcaag taatgaagac ctttaaaaaa ggggatcgtc
     2341 ggtcgaagca cttaaagaaa tactggaaac tacttcaaaa gaatgcgtgg gaattgaaag
     2401 gtcagcattg ctactggcgt ccgtcatttc gagatcatct gacagaagca gagatagtcg
     2461 atcggctatt atcttatgat gattccttaa agcgaggcta cgaagtctac caggattttc
     2521 tttccgttat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
     2581 aggagttgcc agagcactac caaccagtgt tcacgacctt caagaaatac caaacagaaa
     2641 tcaaacgagc gttgcgcgtt ccctattcca acggccctat agaatgtttg aataaccata
     2701 tcaaagtact aaaacgaatt gcctatggct atagaaactt tcaaaactac cgagaacgaa
     2761 tctttttata tcgaggaaaa tattttaaga aaactaagaa tactacccaa ctgactaaag
     2821 ccagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattatt
     2881 ctattgaatt tcttcaccaa cactcattga cgaagagcca aaataagtaa tctaaccgac
     2941 tgttaactaa accattgcag gtcccgttaa agatacttgt gcctgttgtc accgcttctt
     3001 tttcttgtcc acaagcgctt aacacactta ataaagcccc tgctattatt aataataata
     3061 tctttttctt attaaaaacc tcctcatcta tttccagatt agtaaatgta gtaatctgtg
     3121 ctttcttttt tctcaatact aataactaac ttatggggta aacaaatact cgtttgaccg
     3181 ggttttgaga tccatccagt tctaaccgcg atttgatcag gactattatc ttctttaact
     3241 cgaattcttc catctttaat ctcaacaata ttatattgtc ccttcgcagg atgataattc
     3301 acgagtttat gattgatctt atccaaatcg aagcgatcca tttccttgcc atcaattcga
     3361 acgatggcat atttgatagc atcttcagtt gtttcattct ccttttctat agagaataca
     3421 aataagggaa taaaagaggt aataatcaaa atgccaataa ttatgacatc gcctatcttg
     3481 acaccagctt ttcttaaaaa tttcattaac gatatctggc taaacatatt agactttttc
     3541 catggaaatg gtgaaatttt tcggaaatta tctgcattaa gagaatcagt taaacaaccc
     3601 tcaagttgtt ccttaataca ttgttgatcc atcttcatac caataaaaac tagtttatta
     3661 atgcgttcac caaacaacgg atgccaggct tccttcaatt cgggattttt ttgtataatc
     3721 atttgacact caattttagg taaactagct acccaataat ttaatgattc aatactttta
     3781 tagctacccg atgtttcaaa gatcgtcgaa aaatcactat gttgggcaaa ccaaagaaac
     3841 ccttttgctc ttgtaatatt gccaggaaaa tttttatccg taagatgggc aaatttttgt
     3901 gcgtcaagga gtaaccgatt tcgatagata aaacttgaga taccatattc ttctgtttca
     3961 ggaatatgat tttgatagcc aagctctaac tctttgaacc agcccactga ataggaagtc
     4021 tcatctatat taaaaagatt tgtatttaga attttcgata agtctacttc acctttaatt
     4081 gtcctaatta ttttggcatt ggactgtaac ttagcaatta gaccgttaat tttgtaaatc
     4141 gtcttggttg acactaagtc actattattt aaaagtaata tgttacaaca ctcgaattga
     4201 tcaattaaca agccacgaat atcctgttct tcttcaacta cattaccttc agcttttctt
     4261 tcctgaatct tttcacccgt ttcgaaatta tcccaaaaac gttttacatc cactactata
     4321 ataatggcac caatctctaa tcgttgggtc aaatcaatgt ctaaagggtt tttagctaat
     4381 ataattgctt gaataattgg aattggtttg ctgattcctg aagcttcaat aacaatttga
     4441 ttcaagtcag aaaattgcac taattgatct agttcaacaa tcaaatcttc gcgtaatgta
     4501 caacaaatgc atccattcgg catcgaaaca agtttttctc cagttctttt aaaacctttc
     4561 ttgtcgacta ataccccatc aacatttact tcccctatgt catttacaat gacagcaatt
     4621 ttctttccgt gatcaccgta cagaatatga tttagaagta tcgtttttcc tgaaccaaga
     4681 tagccagtta agattgtaat aggtattttt ttcataggga acctctttct ataccttgaa
     4741 tttacacttt aagtgcaaca cataaaaatg agtcataatc gatatccaac tagtataagt
     4801 ttgtcgagaa cactcctaga aggaaaataa ttatgagcca gtatcgtcat cttactatac
     4861 tcgaacgaga aagaatcttt ctgcttcacg aagaaggatt ttctattagt ataattggca
     4921 acgacattca gcatagcccg tttactattt tacatgaact gagaagaaac aagaaaaaaa
     4981 ctattcacct tccaaagcgg aaaaaaatta tgcttatcga agaaaaaaat gcggacgtta
     5041 tcacattcta aatgatccgt gagtttggtc aattgttcgt catttatttg tcgattttca
     5101 gtggtcgcca gaggaaatct cgaatcgttt gaagcatgaa catatggact tttcaattag
     5161 ttttatcacg atctaacgtg caatttgcaa tgatcttttc gatacagaac ccctctcaca
     5221 cggcaataaa ggcctgatcc gttcattgca ccatcgagga aaaacaagac atacaaaaaa
     5281 tcatgtcgag tctcgtggaa agatttgcct tactcattcg atccatggcc gtccaaaatc
     5341 cgcagatgat agaagtgatt ttggtcattg gaaagccgat attgtagctg gcaaaactgg
     5401 tggtacttgt ttagtgactt tgacagacag aaaatcatgc ttccttttag caggaaagaa
     5461 ggtccccaga aagaaagccg cttatgtgag cgagaaaatg attgaacttt taagcagtat
     5521 tccaaatgag gcggtcaaaa cgatcactcc agatagagga aaagaattct ccaatcattt
     5581 cgcagttaca gaagcattga ataatatgcc cttttatttt ccagatcctc atgcccttgg
     5641 caacaatgaa caaattaaaa tacgaataga ctcattcgag aatacttgcc aaaatcgaaa
     5701 gagatagcta cggtagataa ctcaacgata aatcagatga ttgaaaaact gaataacaga
     5761 cctagaaagt gtttagattg gagaacacct tatgaagtct tctttgataa agtgttgcgc
     5821 ttgatttgac aattcaagac ataataaaaa gcagatcaaa tccaaataag ggaaaaatga
     5881 tctactctgg tattatcaat taattggcca agctattatg ccgttttgct aaacgtctac
     5941 ttcttccagt attgaccttg ataaaacaac aatcaggatc ccgttttaat actctatgaa
     6001 ctgtgtcaat actaacatgt aaagttgcag caatatcagc taatgtactc ccatttaatc
     6061 tcatttgctt tgcaaatcga taacgccgat aagcatcaat attcttggaa tccatagaca
     6121 ataatacttt tcttaccatg tatttcctcc aaaaattgaa ttttaatgag ccatcatttc
     6181 ttgagcaatt tcatgtttag acaatgaagc tggatagaaa gtaggccaat tatgcaattc
     6241 attatagagt ttttcttgac tttctccacc aaagaaaata tggaaatgct ctgccttact
     6301 tggtgcaatt ccatgatcac taaattgaat atatttaaaa cgaccagcac ctgcatcatc
     6361 agtttcaaaa ttaaaacgaa caccacggtt tcctttttca taattcaaaa ttttgtatcc
     6421 aacatatttg tatgtgtatt gatgatgttc accgttgact acaaaatcaa tggtgttgtc
     6481 tttaatgttg atagtatcaa tatctgtctt atagccagta gtataataat ctttgtactc
     6541 ggcagctgtc atgtctttat tgatttttgc tttgtaatcg aaaacttcat ccaaaattcc
     6601 atcttttaac aacggataaa cagattgcca ttcgcctgta taatcactca attcgcgatc
     6661 cttcacatcg gcatctttaa agtacccatt agcaactgtc ttttcatcct tcgcttcttt
     6721 ttctggaact acctcttcac cagcaaccat agttgttttt tctaacgctt ttagattgtc
     6781 ttccattact gaaacataat cttcaccatt atccatttgt tcttttgtaa gactttcaag
     6841 aggattcagt acttccaatt ttatcccagc ttcgttggcc aaagtttttg caattttatc
     6901 attagcattc ttttcaaagt aaatataatt gatcttattt tttttaacat actcttttaa
     6961 ttcagctaat cgaccagaag atggctcttc ttcaggactt agtccagcaa taggaacttg
     7021 tttcaatcca taatccaaag ctaaatatcc aaaggctgcg tgttgcgtca caaaactttt
     7081 ttgtttcgct tctttaagac tagtggtata gtcagcatcc aaccgtttca gtttagtcaa
     7141 atacttttca gcatttgtct caaatacttt tgcttttttc ggatacaatt taactaactg
     7201 atccttgata tttgaaactt ccttaattgc cattttgggt gaaacccacg tatgaggatc
     7261 tagttcatga tggtgatctt cttctccgtg atcatgatct tcttcttcac tacctggcaa
     7321 taaaatcatg tcttttgttc cttctactac attaggccca gctttcttcc aactttcttt
     7381 agcttttggt acccatgatt ccatgttttc gttatgatat acaaaaacat cagcatcatg
     7441 agcttttgcc atatccttag ctgagggctc atagtcatga ggttctgatc ccgctggaac
     7501 cattaaatcc acgttcgctt catcgccgac gatgtttttg gtgaaatcat acattggata
     7561 gaatgttgtt acaatttcta attttccatc tttatcattc gatacttttg catcattagt
     7621 gctggagcaa gctcccaata atcctagagc tgctatccca ataagtccta cactgatttt
     7681 cttcattctt tttccttcct taattatgct aatttcttca ttttatttcg tttatgttct
     7741 ttgataattt ttaatgccct ttttctagtt ttaatattcg ggcttttaag tcgtcgatat
     7801 gcactagata tatccgtttt ctccattaga aattaccccc aatcactact tatcattatt
     7861 tgctttccgc cagtaattta tcaaaactct gtttgatcct ttcttcatcc aacatttttc
     7921 cgattagaat aatagtggtt tggtcttttt gtcgagtggt gtattgaaaa ttaacctgtt
     7981 cgtttacctc ttgcatggca actataaaat cctgctcttg taaagcaatc aatcctttaa
     8041 aacgatacag gctcccttgg ttggttaaaa tcagccagtc aatccattgg agtaaaaaat
     8101 ctttttttat gtcttgatct gaagttaaga gaattgattt aaactcatgt acttgatggt
     8161 gttgatgata gtatttgcca ttatagtgat tttctgatga atcactttca aaaactaagg
     8221 gatgattaaa ttttttcaaa ttataaaaat cctttttttg aatcgtttca ctatgtgtga
     8281 acattcgaat atcggataaa gggttaatac ttttgatttc tctctgtaat ttttttagtg
     8341 cagataaatt caagttttgt tttctagaca caaaaatgcg atctgccatg acaagttgct
     8401 taagggcttg tggttctctt tcaataagtt caaaactgtc actatcaata acagtcaata
     8461 aactatcgat atatactctt cctcttagtt gaggcgctgt taaaagtgtt tgaatgattg
     8521 gttggggatc tgctatacct gtagtttcaa tgatgatttg tgaaattggt tgttgatgtt
     8581 cttctattgt ttccagtaaa gaaatcaata ttgctaataa atcactgcga agactgcaac
     8641 acatacatcc attatttagt tgtaacaccc gttcctcgct atgaactaat aatttgtgat
     8701 caatcccaac ttcaccaaac tcattttcaa ttataataat ttcttccctg gggtagggtg
     8761 agacttgcaa ggcttgattg attagcgttg tttttccagc ccctaaaaac ccagaaacga
     8821 ttgtaactgg tatgcccatt ttgtcacctt tttaccactt agctatttca cttctttaaa
     8881 aattgcccgt ctgcgcaatt ttggcgaata cttttttaat tctaaccgtt cagggttatt
     8941 ccgtttattt ttgctggtga gatacagacg ttctccagtt tcgacacatt ccaaaataat
     9001 attttgacgc ataaatcttc ctccttcaat acttatcgat cttcaagata tgcttgaatt
     9061 tgttgtgcat cttcaaaagt taactcgcca gattccaatt gtgaaactaa attttctttc
     9121 atatcaacag gtatctccca ataaccatcg atacattgaa ataactcctc gtcatactct
     9181 tcaaaaattg gatagattct taaaaaatca tcaaatgata agccttggtc ctcatagtaa
     9241 tctttgacta atccattttc aattgcctgt tgggtcggta aacccgtttc agtaataatt
     9301 cctctctcta ccatcgaacg ataaaaagtg catttatcta gcgattgctg ggtcgtttca
     9361 agataatact ttaatccttc ttcaactaaa ggtgggtaaa taatttccat tttgattccc
     9421 tcctaaaagt tatatattaa actattttga agctccttta attactttct ttccattata
     9481 gtaccctttt aaagatacat gatggctacg tctataatca ccattcgctt cgtcaaatga
     9541 aatttctggt tttactacag tttgatgagt tcttcttaac cgtttcttcg ccttagatgt
     9601 ttttcttgct ggtactgcca tgatatttcc tccttttacc aacttgcttt ttttactcca
     9661 ggaatcaacc cttgatgggc aagttctcta aacttaatac gggacatacc gaactttcgc
     9721 atataccctc ttggacgacc atccgtctga tcacgaagcc tcagacgatt cggatttgaa
     9781 tctcttggca atttagcgag agcggtgcga tctcctgcgg cttttaattc ctgacgttta
     9841 tctgcatact tttcaatcat cgccatttgt ttctttgctt tagcaatttt cgactttttt
     9901 gccatatacc tttatactcc ttttattttg atttagacac ctcaaatcgt aatcgttacg
     9961 ttttgacact gatactttac caacaagagt tgctcttgtc aacaataaat aacatgaaat
    10021 aaaactcata tgaatagata actatttgaa aacgaattta tcggtacata gagataatca
    10081 aatcatttaa aagaatatgc agtttaggct ctgaaaaata agaattcact tttattagtc
    10141 caatcaaaca caaataccgt tttaagtagc ttttcctcta cttaaaacgg caatatgaaa
    10201 gaaactataa ctttttattc aatcttttat gtttaactgg tttatcgcca attttttaaa
    10261 actttgatta ttccttatat tataagaata gacattaagt gaactaattc aatcatactg
    10321 attctttcgt gtattcaaaa ttagatgctg tttaataaaa ttattctttt tgaaaaagaa
    10381 ttataactat atcccaaaaa ttgtaggtta atttaaagcc tatttcatca tcgtgattga
    10441 gcttcttttt catagacgaa tttctcagat agtttagttt tctttatgag atttttgtat
    10501 atttctgacc ttcgatacaa ctcattatga gtaccttgat caactagttt tccttgatcc
    10561 atcactacaa ttttatttac cttttcaata gacttcaagc gatgcgatat aattagtact
    10621 gtcttatctt gtattaaacg atttaaactt tcctgaatac gtttttcatt ttccgcatct
    10681 aaactggcag taatttcatc taaaattaaa attggcgcat ttttcaaaaa agcccgtgct
    10741 atggataggc gttgtcgctc tcctccagaa agtgaacttc cgttttctcc gaccaatgta
    10801 tgataaccgt caggtagttt ttcaataaag tcttcgcagt tagctaaacg agctgctttt
    10861 ttcacttcct catctgacgc ttgactattt ccaatacgta tattttccaa aatacttgta
    10921 ttaaacaaag ttacctcttg aaaaacaata gcgattttgc taaacaaaga agctggagat
    10981 acacgtttaa tatcataccc atcaattaaa atacagcctt catcataatc atacagtcta
    11041 gacactaatt tcaagatact agttttcccg gaaccactag cacctactaa cgcagtcact
    11101 tctccttgtt tagccgtaaa agagatatga tctaaaattg gggtattgtt atcatatgag
    11161 aaagagacat ctcgcaactc aatgtcaaaa gatttcagag gactatctga tccctcttgg
    11221 atactggttt ccttcatagc tctaattcgc tgtattttag gagctaaata aaatatttca
    11281 agcactgctt ctttcataga atcgaaggaa tcctttattt ttattgcggc tagtaagtaa
    11341 ccaacgacgt agagtatagt cacttcaccc gttaacagta agtgaacacc tactagaatg
    11401 actactgcta aactaacata tgagaaaata gaggataagg ccatgactga aaaagtactt
    11461 aattctacct ttaaatgaat cctttcactc tcttccattt ttttgaaaag tctgtcttga
    11521 acttttttag atagattaaa gctattaatc tcttgctgca attctatagt ttcttgaaat
    11581 tcttccgagt tctctctcaa agtatcgtaa tatttagtat tggctttcgt ttgggatttc
    11641 tttgataaca agattaagac aaaactaaat aacgttggca aaataacagc taatcccatt
    11701 ttgacattac caaccaaaag catcactgaa ataaaaggga aaaacagagc cataccgccg
    11761 gctttaggta ttgcatgact catcgcatgc tcaatacctt caacgtcaga catgatagtt
    11821 tgagataaat ctgataaatt atgttttgaa aaataggata atggtaaatt tgacaaatcg
    11881 tctgcaattt gcactcttaa atgcgcactt tctttataag tactattata taagttttca
    11941 tactccctat ctaacaacca atacatgaaa agcaaagtaa caaacgatac ggcaaaataa
    12001 agccaacggg gctttgtatt ttctaaaact aattcgtcta tcagcatcat tataatgaat
    12061 gctggtacca tattaatgca gtatacaaaa aaactagaaa tagaagcttt agttaaagct
    12121 tctgcgccct gatcacttaa agcaaatttg tttttataaa aacttttcat tgtcaagcct
    12181 ccaatcattt gcattctcat aattagtgac caattgttta tacacttgat gtttagccat
    12241 taattcatta tgatttccac gttctactat tttcccttct gacaagacaa gaatttcatc
    12301 tacagattta atgctggata atcgatgagc tatcataata actgtttttt ctttcatcag
    12361 atgttgaaaa gccttttgta gttcatactc attatctgga tcaatagatg cactggcttc
    12421 atccataata acaatctttg catcttttaa aattgctctg gcaatagcta tacgttgttt
    12481 ttctcctcct gacagataaa ctcctttact accaattaag gtatgctctc tttcgggaaa
    12541 tttttctaaa atcgattcgc aaccggctaa tcttagcgcg tcaaatactt tacttgctgg
    12601 cgcttgagga tttgctatag aaacgttatc gtaaatactc tgattgaata gtttcgtatc
    12661 ttgaaagaca aaagcaattg tgttaataat tgtgtctttt gaatagtgat ttaaaggctt
    12721 accaccaatt ttaatctgtc ccttatccac attgtagaaa cctgaaatta gtttagctat
    12781 cgtactcttt ccgcttcctg attttcctac taatgcataa attctttttt gttctaaaac
    12841 taagttgaaa ttttctaaaa cacactcttc tttgtatgaa aagctgacat tttcaaatgc
    12901 aatgtcacaa tttttaatct gtgtctcgtt cccatatttt agttggtctt gcttcatttc
    12961 taaataaagg gagtctagtg tttcgactgc atatcttgct tcaaacaagt gcatacttac
    13021 atacattatt ctcatgaaag aaacaaaaat aattccactt aaaaaaaaca acatcaacaa
    13081 gtcaacagct aatatcttgg gagtggtaag agaagttata aaaaaggtta taggaactat
    13141 caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga acaaaaggtg ttttcccact
    13201 caaagaatac ttatatgcaa acttagcata atcgttaatt gctttattca aggctttaaa
    13261 tgatgttact ttagcaccaa atatctttat aacttgaatt ccgcggatat attctaccgt
    13321 ttcagagctt aacacatcta acgattcttg atatttttga ataaaatttg tattctctgt
    13381 cattttcttt aacaagaata caccaattac taacatggcg attagaacca atccaatttt
    13441 aaagttaatg aaaaagccta aggcaactga taaaacaggt gttactatgg cttgagcact
    13501 atctggaatc aagtgagcaa ttgccatatg tgttttagcc gcgttgtcat caattgtctt
    13561 tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa cttgcttctg ttaaaccgct
    13621 aattcccttt tttctcaaat tagtctctaa gcgaaaccca aggatatgag aaaataagcc
    13681 agaaaaaata taaaataaag ctcctaaagt taaccaagca acagtttgaa ccgcataact
    13741 cttagctaaa tattcatctc ctacaataat tagagactga agaaatttgt aaatactgta
    13801 atatccatag actgttaata cgacagaaaa atcagaaaat aaagtggcta accatccgta
    13861 gtatttcatt ttagggacat aactaaataa ttttttgtat actttcattt tttcctcccc
    13921 cgcattcaat ttcttctctt attcgtttac aactgttaat gctttccgta atagcgtaaa
    13981 ttagagatgt acaaacagat aaaaggtaaa ctacgttcta ttttttgtat ttttttaaga
    14041 cttttcttcc aatcaataca ccaatcatag ataaacttgc tacaactaac actgaaatga
    14101 ttccccatat acctaccgca tagtggatca ttgcatccat tgttgcagca gattgtcctt
    14161 tatctatcca atcttgacga aaacgatctg taaagaacca tataggaatc cactgtccgg
    14221 cagcactgcc taattgcatt agagcccaag acgcaacttg aggaattgct cgatcatatc
    14281 caaatacaga tagaataata tcagctaaga tcgccatact aatcgaactg ataaaccaag
    14341 gtaagtacgc cccgcccatt aacgtaaaaa ataacaataa aacaattgtg taaatagtaa
    14401 aaattccttt tctagggtag ttatagctaa tataaacaaa tatgattcca gataataatc
    14461 caaaaatccc gggggatatg ctgtgaccaa acgccccacc aatactacta accattattg
    14521 aaaaaaagta aattactagg gaaataactg tagctaaacc aataaaaata taatcttttg
    14581 ttttcatata tattctcctc aattctattt cgttatgtca ttaaaaatct gttctatcaa
    14641 agttgaatct tctac
//
LOCUS       DAYYME010000064        14497 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000064 DAYYME010000000
VERSION     DAYYME010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.065"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            42..1955
                     /locus_tag="ACHBFW_001657"
     CDS             42..1955
                     /locus_tag="ACHBFW_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9317962.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            1960..2961
                     /locus_tag="ACHBFW_001658"
     CDS             1960..2961
                     /locus_tag="ACHBFW_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9317963.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(3163..3369)
                     /locus_tag="ACHBFW_001659"
     CDS             complement(3163..3369)
                     /locus_tag="ACHBFW_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFM9317964.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(4015..4863)
                     /locus_tag="ACHBFW_001660"
     CDS             complement(4015..4863)
                     /locus_tag="ACHBFW_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9317965.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            5027..5929
                     /gene="gnd"
                     /locus_tag="ACHBFW_001661"
     CDS             5027..5929
                     /gene="gnd"
                     /locus_tag="ACHBFW_001661"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFM9317966.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            5931..6932
                     /locus_tag="ACHBFW_001662"
     CDS             5931..6932
                     /locus_tag="ACHBFW_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFM9317967.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            6929..7213
                     /locus_tag="ACHBFW_001663"
     CDS             6929..7213
                     /locus_tag="ACHBFW_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFM9317968.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            7246..8043
                     /locus_tag="ACHBFW_001664"
     CDS             7246..8043
                     /locus_tag="ACHBFW_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM9317969.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            8057..8890
                     /locus_tag="ACHBFW_001665"
     CDS             8057..8890
                     /locus_tag="ACHBFW_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9317970.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            8908..9765
                     /locus_tag="ACHBFW_001666"
     CDS             8908..9765
                     /locus_tag="ACHBFW_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9317971.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            9787..10296
                     /locus_tag="ACHBFW_001667"
     CDS             9787..10296
                     /locus_tag="ACHBFW_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM9317972.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            10333..10734
                     /locus_tag="ACHBFW_001668"
     CDS             10333..10734
                     /locus_tag="ACHBFW_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317973.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            10846..11199
                     /locus_tag="ACHBFW_001669"
     CDS             10846..11199
                     /locus_tag="ACHBFW_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317974.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            11233..11676
                     /locus_tag="ACHBFW_001670"
     CDS             11233..11676
                     /locus_tag="ACHBFW_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9317975.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            11693..12778
                     /gene="uxuA"
                     /locus_tag="ACHBFW_001671"
     CDS             11693..12778
                     /gene="uxuA"
                     /locus_tag="ACHBFW_001671"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM9317976.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            12771..13484
                     /locus_tag="ACHBFW_001672"
     CDS             12771..13484
                     /locus_tag="ACHBFW_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9317977.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(13691..13840)
                     /locus_tag="ACHBFW_001673"
     CDS             complement(13691..13840)
                     /locus_tag="ACHBFW_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317978.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            14236..14436
                     /locus_tag="ACHBFW_001674"
     CDS             14236..14436
                     /locus_tag="ACHBFW_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9317979.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         5010 a         2282 c         3084 g         4121 t
ORIGIN      
        1 gtttgcgttt acatataatt ctcctagaaa cataaaaagg agtgaatagc ttgagaacaa
       61 aacaagtatt aaagggagcg ttggtcatct tatttgctgt aagtacaagc gtcccgcaat
      121 ggacagtata tggagagagc atttcttccg aatcggaatc tcgggaggta gaaaccacag
      181 aaactactga tgctggaaat gaaacgaaat cgagtacttc acaaacagaa acaaatactg
      241 aaacaacggt tgagacatct gttactagca cagatcaaga aacgaccgct accacagata
      301 accaacaaac agaagaagca acagctacaa aaatgtcaaa cgacgaatcc tcttctgcta
      361 aagtaagtga caaagagaat gggacagaaa atgatgatat cggtaaaggt gaaaatttca
      421 atggtaaaga atatttgaat tacttcggta aacagatgac agaaatactt aatggaccaa
      481 cagaagacat caagtcaaaa ttaatagagt tattagtcac tgagcttgct gttaatgatt
      541 atggtgtaga taaagagatt atcaaacatc tgattgctac ttatctattg tacgatatcg
      601 cttttgcaaa aaacagtgtg ttgatgtcga tgcttggagg tacagatgat gggacaacgc
      661 ctacagaaaa cacaggcttc tatccaaata caaaacaact tcatcaaact gtactaaaca
      721 aacaagatgg caaaatgatg gatgtatatg catattatgt cgatcaaggt tcagataaaa
      781 cagtcatgat ccatggcgga tttcgaggga attggaataa cgggatcgtt acagaagaat
      841 acaatgattt ttataaggcc gggtataatt tattatttgt tgattccaga gcgactggta
      901 atagcggagg cgattatgtg acctatggac aatatgagtc agatgacgtc ctttactgga
      961 taaatcaaga agttcgagag cggccatctc aaaagatttt actttatggt ggttcgatgg
     1021 gagcagctac gatgatgtct gtattagcga aagatattcc tgtcaatgtc aaaggaatta
     1081 ttgaaaattg cggatttgca tcaattgatg aacaactacg ttttacgtac agccagacag
     1141 tagcccccgc actaccggat gccataaaaa atcagttaga catcattggg gatcaagagc
     1201 atgaagattt atttatgggc cttttgaaac aatactattt tgatcaggag atgcatttgg
     1261 atacaaaagc tgctctacct acgattggaa tgtcagattc attgcctaaa ttgattatcc
     1321 atggaacagc agatgatgta gtacctgtgt ctaacgcgca aaaattatat gagttatcag
     1381 gagggtataa agatctttta ttagtagaag gagcagggca tggaaaggca caggaagttg
     1441 accatgctgc ctacacgaaa catgtaacgg actttttgaa agtagtgttc catgatcaga
     1501 taaacgtaaa gtatgtggat gaaaacaata aaagtctact caaagaccag gacgaaatcc
     1561 agctttacgg agcttatgga gaaaattata tgacagagca aaagacattc gaaggatacg
     1621 aattagcaaa tgttgaagga ccgactgaag ggatttttaa tgagacgata ccaactatta
     1681 ttttctatta caaaaaaatc ccagtagctc caccaaaaaa gcaagatccg acagagaatg
     1741 cagacaataa gggaaagaat gaacctcgag aggatcaatc aagtgatgat acgcaaccga
     1801 ttcagatgac agtgaaagta caaaaaaaga cagatgcaaa aaaaagaatg ttgccgaaaa
     1861 ctggagaaaa gaaacattcg cttctcattt attcaggtgg atttttatct gttagcacga
     1921 ttatcacatg gatttggcga agaaagagag ggtaaatgaa tgaaatggtt caagcgaata
     1981 gtgattgcag taccagttat catcgtagtt attttagtca ctgtattttt gattaatcaa
     2041 atcacaccaa agccgattag cttgcttgtt agaaaacaat ttgacacaag tgaaaaagaa
     2101 caagtatatg cccgaccaga tgatttccag caaaaaatcg aacaaatcga tataaaaaaa
     2161 gaaatatcct atccttcaaa gtacaaaaat aataatatgg atatctacac accgaaagtg
     2221 gaaaagaaaa aattaccgat tcttttctgg atgcatggag gcgcatatgt aggaggggat
     2281 aaaaatgatt gtcgtgatta tttggaatat ctatgttcag acacgcagca aatcatcgta
     2341 aatatagatt atgaacgatc accggaagca aaacatccta caccagttat ccaattgaac
     2401 gaagccattc aagcagctaa aaaagagata aaagatcaag cagattggtc gaatatttcg
     2461 attggtggaa attcggcagg tgcacaaatt agtggagaat atttgttagc acttcaaaat
     2521 gaagaaatta aaaaagctga tcatcttgat ccgactttgg agaacaagca gattacgaag
     2581 tttatttctt tgtctggtct gttggatcct tctgcactta ctcaagtaag tgataaaatc
     2641 tctagctttc tttatgaaaa atgtggatgg gcttactttg ataataagga ctttgaacaa
     2701 tcagagaagg tcaaaagttt agccttggtc agacatgcgg atcgttggac gcaaaacaca
     2761 ttcttaacgg atggaaatac aaatacgttt acgaaacaaa tggaagaagt aacttctgta
     2821 tttgagaaag cgggggtgaa agtcacaaaa gtagattatg cggagaaaaa tgctgttttg
     2881 aaccatgaat atcagtttga tttcagcaat aaacaagcgc aagaaacata tcaaaagtta
     2941 gtaatgtttt tcaaggagta aagtcttatg atgcaaaaga ggttgtaatc tgtcacttag
     3001 ctccaagtca caaggagcaa tttcaataaa caagcttttc atggtttatg aatattgtaa
     3061 cttgtgactg aggagttaga gatggcaagg ggcacctttt attcgaaatt tgggaatatc
     3121 acacgattat tgtcacgatc tcttttacgt aaattttata agtcagattt gttgttttga
     3181 tcgtttaaaa aaagcaacaa atccgaagcc tgcggaggca agaagggaca aaaagccgag
     3241 tggtaagcaa aagaatgctg gttccactaa tattccgttg ttattgatat aaggctggga
     3301 acaaagatga atagcaaata aaatcaatgc actagcgaaa aaaagcaaag aaatatggat
     3361 agctttcata agcagcacct ccttttttat aattgtatga taaatagatt aagaatctat
     3421 aaatgtgaca agtttgttag gatgtgaata aaatgatcat ctagcgaact tgaaacaaag
     3481 atggctaatg ataagcaatt aatgattcag caatcagata ggtacaaagt ttaccatcat
     3541 acgtctagac caataataga aaatatggga atataataga aaagatttag gataccccca
     3601 aaagaatttc cccaaaaatt cgatgtatcc taaacttttt tattttacat aacaatcata
     3661 tcaactgtga aataaaatta caaatgataa cctaaaatga ggccaaaagc cagtgttatc
     3721 aaggaaatac ttaattcacc aaaatttttt ttaatttttt agaaaataag aaacaaaaat
     3781 aataaaaaaa agaaaagata aattttttac tactgtatac taactttgtt atcgcatcta
     3841 acaagtaact cgaataatac caatacaacc ttctccaaag atgcgatgac atacgttcta
     3901 acccaccgac tattgatgtt ctgtaccacc cttgcagaac caaaaaagcc actaccaaat
     3961 atttggtagt ggcttttttt gttttatgga tttttcgctt tttctgttat ggtgctattc
     4021 tttgatttca gaaatagtat gtcgaacttt agcgatagaa cgacgtgatt ctttttcgtt
     4081 gtaaaacatc aaaatcacat atagagagtc caaaatgctt agttgagaaa tacgggaagc
     4141 taaagcttca gatcggaatt ctgtttcttc agaaatagaa ataaaaacga catcgccaag
     4201 tttagcaagg gaagaattag cttggctagt aataacgatt gtttttgctc cagtttttct
     4261 tacggtttca gcaatatgga ttgtttcttt tgattgccct gtgtgagaaa tcaaaacagc
     4321 acagtctttt tccgtcaata aagaagcttc catcaattga atatggtaat ccgtgctgtg
     4381 agaagttgcg ataggggaac gcaaaaattt atgataagcg tcagttgcta caatctctga
     4441 accgccaaca ccgaaaaaat agacacgttt agattcattg ataatagtag ccgctttttt
     4501 tagatccttt tgttgtaaca acttttttgt gtcatttaaa gttgaaatac tagactcgaa
     4561 caccttttgc gccatagtga gctcagtatc tgttttctga atattttcgt ggatagaaat
     4621 agcagataga tcattttctt ggatcaacat ggccatttta aaatctttaa atccattata
     4681 accaagtttt tttgtaaatt gaaaaaaagt agagtcagca atgcccagtt cacttgctaa
     4741 attgctaata ggactatgac ttactttgct tggattttct aaaatataat cggcgatagc
     4801 ctgttctttt gggctgaagt cgttgtacat tgttttgatt tgtagtctta tggagacgct
     4861 catgagaatt cctcattttc ataataagat tatcattttt tccgttctca ttaatgttaa
     4921 agatacaagg agagtaggaa aaaatcaaac aaaaaatatt tttgtaaaat ctatttacaa
     4981 aaaaattttt ttgtataatg agggtataag aaatggaggc gctatcatgg aaattggtgt
     5041 aattggtttg ggaaaaatgg gattgaattt agctttaaac ttaaaagagc accatcacaa
     5101 agtacaagga ctcgacattt caagtacagc tgttagtcag gcaagagaaa atgacattcc
     5161 tgcttatcaa gagatctctg aatttctagc tacttttgag cagaaaaaaa tcatctggtt
     5221 aatgcttcct gctggagaga taaccgaaaa catgttaaga aaactttttc caaaactcgg
     5281 accaggagac attattattg aaggcggaaa ctcttattat aaagattcaa tccgtcgagc
     5341 agaagagttt cagaaaaatg aaatcggtta cttggattgt gggacttctg gaggaatcga
     5401 aggagcacga catcaagcct gcttaatgat tggaggagat aaagaaactt ttctatccgt
     5461 ggaacaatta ttcaaagatg tagcaattga aagaggatat ctgtatgctg gtgcatccgg
     5521 aagcggacac ttcttgaaaa tgatccataa tggaatcgaa tatggaatga tgcaagcaat
     5581 cggtgaagga ttccaacttg tacaaaaaag tgagtatgct tttgacttgg aacaagtcgc
     5641 acgtgtttgg aatcatggtt cggtgattcg cggctggctg atggaaatca tggaagatca
     5701 gttaaacgaa catcccaatt taagtaatta tcggggaatc gtttctgcat caggtgaagc
     5761 aaaatggacg gttgaaacag cgctagaaat ggctgttcca gttccagtaa ttgcaactag
     5821 tttgttcatg aggaatcttt cacaggaaga agatagtttt tcagcaaaag tagtttcagc
     5881 actaagaaat gggtttggcg gacatgaaat agttgaaaag ggtgagtaaa atgccagatt
     5941 atcatatagc aatcgttaat tccagcagtt tcggaagagt ctttccacac cacttggaaa
     6001 gattaaagtc agttggagac gttcatttct ttactgttga tcaagagatt ccaggaaaag
     6061 aactagcgga gttacttaaa ggattcaata tcattattgc aagtgttacg ccattttttc
     6121 gacgagattt ttttgaaaac aaagatgaac tgctattgat ttcccgacat ggtattggtt
     6181 acaacaatat tgatttaaaa gcagcaaaag aacaccagac aatcgtatct atcatacctg
     6241 ctttaattga acgaaatgct gtagcagaaa ataatataac taatttgttg tccatgcttc
     6301 gacgcacgac acaagcacaa gcaagagtca aggacgatcg atgggaagat cgggcggagt
     6361 tcgtcggtag gacgctgttt aacaaaacag ttggtgtgat tggtgcagga aataccggaa
     6421 gcggagtaat cgaaattttg cgtaacggtt ttcgctgtga tgttctagct tatgatcctt
     6481 acaaatcaga tttatacatc gaaagctttg gtgcaaaaaa agtagaactt caagaattac
     6541 tggaacaatc agatatcatt tgtttatgtg cgaatttgac agaagagaac tatcacatga
     6601 tttctgataa agaagtcgat cagatgaaag aaaatgttta tctatctaat agtgcaagag
     6661 gcgctctagt tgatgaagaa gcgatgatta atggtctgaa aagcggaaag atcgcaggat
     6721 atgccacaga tgttttagaa gaagaaccgg gacgtcaaga tcatccgtat ttagcgtttg
     6781 aacaagtagt gattacgcca catatatcgg cctacacaag ggaatgttta gaagcaatgg
     6841 gtgaaaaatg tgtcagagat gtcgaacaga tcagtcagca ccttcttcct gaacgttctg
     6901 tccagacaaa gagcaactat ataaaaggat gatgaagaaa atggtgaacg gtttaaaagt
     6961 gaaaacaggc cctcaatttt atttatatga agaaggtggt atttcaaaag tttctgatct
     7021 cttgaaatca tacggcgcga aaagagtact ggtcacccac ggaacggtat catgggaaaa
     7081 ggcgctacct aaattagtat ttttgaatga cgaaacgatt caattttttt atcatcgtta
     7141 ttctggagaa tgtagttatg ctgaagccag acgaatagct acaatcatca aaaaaatgaa
     7201 atcgatttcg tagttggcgt tggcggagga aaactgaccg atctagtggg ttatagtgcc
     7261 aatcttgcaa gaatcgattt cggagtcatc cctactttag ctagtaattg tgctccatgg
     7321 acacctcttg ctgtcatgta taaggagaat ggacaagcag aagggaaaac agaacacttt
     7381 tacagacaag ctgctttttt tataacagat ccccaattag tgattgattc ccctgttcca
     7441 tatttcattg ccgggttagc agatacacta gcaaaatggt atgaatctga gctcatctta
     7501 gaacaagatt ttttgcaaga tgaatcattt ttgaagctag cacaagatac tgcgaagatc
     7561 tgcaaagaag agattttgaa tcattctgct aaagcaatcc aagatatggc acaaagaaaa
     7621 ctgacatcgg agttcaaaca gctttcagag attatctttg ctgttgctgg tttagttgga
     7681 ggattaggag ataaatacgc aagaaatgcg gcagcacatg ctatgcatga cgctatcagt
     7741 aaatatattc cggaaagcca tcgttttcta catggagaaa aagtagcgta tgggattttc
     7801 tatcaactgg cactagaagg tcgctgggaa aaaatagacg agttatatcc tttgtatcgg
     7861 gaattagaac tgccggtttc tctagaacaa atgggattat ttccgaaaga taaacagata
     7921 atagataaaa tggctgaatt tattgattcg aaagagaaag tgcatttgat tcctatagag
     7981 gtaacacaga aatcactgat acagtcaatg atggaactgg aaggttatat cagaaagatc
     8041 tgaaggagga ggacacatgg aaacattaag tgtagtacaa agtttgttga ttgctttatg
     8101 ggtagccgcg gttatgtccc gctggttagg tggaggagcc acgttgacct tacgtttttc
     8161 tcctttgatg actggattag tcgtcgggtt gatcatgggg gaagttgcac aagcgatgat
     8221 tgttacagcc gctttgcaaa tgatttatat gggggttttt tctccaggag gatcaatgcc
     8281 agctgaacca tctattgcag cagctatcgc agtaccagtc gcattattag gggatctgtc
     8341 gccagaagca gcgattgccg ttgcggtgcc agtcggattg ctcggaagtt acttgtatca
     8401 gtttcgtttt ttcatcaata cttttttagg taaatataca gacaaagctg tagaagattt
     8461 gaatacaaaa gctatggccc gatcaattat tctttatcca acggttgcct catttttatt
     8521 attcgttccg cttgttttca ttgctttata tttaggggcg ccattgatcg ctgatgtaat
     8581 caaagcttta gagggaacag tcgttatcca cattcttgaa gtagtaggtg gagggttagc
     8641 ggcaatcggt atcgcgacaa ctgtatacgt tatcggtcga aaagattatc tggtcttttt
     8701 cttccttgct tattttatga gtgttgtttt gaaatcttta gatattacga tggttactta
     8761 tgccattttt gggattatta tcgctttgat ctttgtccag tcgcaaaaag ggaaacaagt
     8821 acagcctgcc acaagcaata acaattcagc atcttttgat gacgaagatg actatgatga
     8881 cggattttaa tttaggagga atgggaaatg gctgaaataa tggataatca aacaggtgct
     8941 cctgaagaaa tcacgaaaaa agatgtaaca aaagcctatc tgagatggca ttttgcaaac
     9001 gaaattcctc actcttttga acgttacttg gcgccatcac tactatacgg aatgatgcca
     9061 atcttgaaaa aactttataa agatgaagac tctttaaaag cagcatataa acggcagtta
     9121 ctgttcttca acactcaatt aagctggggt ggcggtgtga ttactggcct aatgtcttct
     9181 atggaacagc aaagagcaga agaagagtat gaagaaaaag agatcttgat gcaggatgac
     9241 ttgatgtata acacgaaagc gggattgatg ggggcactag caggaatcgg agatgcaatc
     9301 gattcaggaa ccgtccaata tatttttatc gcaatcgcgg taccttgggc acaacaggga
     9361 agtcctttag gcgcactttt tccttttatc tgctttgggt tgtatcaggt tttactaggg
     9421 gtatttttcg caagacaagc tttcactatg ggccgtaatg ctacaggcct aatgcaaaac
     9481 actggtgttc agacagcgat tgaaatgcta tcagttttag ggttgtttat gatgggggtg
     9541 cttgcgggaa actatgtaaa agtagaatct tcattgaaat ttgcgatatc cggacgtgag
     9601 tttgttatcc aggaaattct tgatcaaatc gtgccaggga tattaccttt agctgtcgta
     9661 atgggtgtgt acttgttcta tacgaaaaaa ggattgaaag tcacacaagc attattatgg
     9721 ctgactggaa tattaattgt gctagcagga atcggtattt tataaaaaga agggaacgtg
     9781 aataagatgg gtgtagtcaa tttagcgcgt gtggatgagc gtttgatcca tgggcaagta
     9841 atggtaacgt tatctcaaaa aagtggagtg aattccattt ttgttgtaga cgaagttgta
     9901 gcaaaagata aatttatgag agatctttac aaaaatgcag gaagccgtac aggacaaaaa
     9961 acaattgtca ttacaccaga gaaagctaaa ttctattggg atgaatatca atttaaagaa
    10021 tacaattgta ttttgatcgc aaaaacagtc caaacgattt acgacttagt aaaacacgga
    10081 atccccatgg aagaattgaa tattggaggg attgctcaaa aaaatcctga tacagatctt
    10141 ttagtgacga aatctgttta tttaaataaa tcagatgctg aaaaattgaa agaaatgaat
    10201 gaacaatatg gcgtaaagaa tatttatttt caagccactc catctgcacc taaaacagga
    10261 ttaaaagatg tattgaacaa gtttgatttg tcataaacaa atgcttagaa aaaagataaa
    10321 gagggcgaaa aaatgaagaa gaacgagctg tttcgtgatt gggaatttcg ttatcgctat
    10381 atttaccgta agcgccgtac aaaaaagagt aaacagcgtt ttctttcagc attagtatca
    10441 gatatttaca gtatgagaac agatgtgaca gtcattgctt acgatacgct agcctaccga
    10501 tcaaaaaata tttatgttgg agatatcgaa aaagcagaga aagttatctg tacttattac
    10561 gatacacccg ttcatgcatt aggaagttat ttcatgtttg attggaaaga ccaaaggaaa
    10621 aaaacaatct actctatatt attgtccttc attttgttgt tcagtttagg ttggtgggga
    10681 atgatgatat ataacaaaaa tcctcatcac gtattcgatc tgctatccgt ataaacaggt
    10741 atcactgtac tagcatttgg gagctacttt ttcttattgg gaaaagcagc tcgcggatgg
    10801 cctagcagac aaacttttat tcgaaatact tcatccatat taacgatgct agaaatgatc
    10861 cgtacgattg atgatccgag tgtagcgtat gcgtttgttg atgaaggctg ttatggagaa
    10921 aagggcttag acagcgtgag atcgggtatg aaaaaagagg ctattctttt ttatctagac
    10981 tctgttgggg cagatacacc gctacagttt tcgggaaatt atttttcaaa taaagaacag
    11041 tggctgaaac aagttgataa gttgaaagaa aaaaacgtca attatatttt tagtgcaaga
    11101 aaaaagcaag cgcagttctt ctatttgacc aaaacagatt tacgagggaa aacatttaat
    11161 tggcaaaatg ccaatcagat tattgctctt tttcgataag taaaaagtgc aaaacaagga
    11221 gtgagaagac taatgttgga aatcatgatc gttacacatg gtaagttgag cgaaggactg
    11281 aaagatgcgg cggaagtaat tgtcgggaca tcgagccata ttgaaacaat gtgcttagag
    11341 tctggggagg acatccaaac attcggagag aaggtcaaag aaaaattaac tatgatcaac
    11401 acagcagaac ccatgctagt atttgttgat ctaattagcg caagtccgta caatcaagcg
    11461 ctcttggcag tcaatgaact gactgaagaa aagaaagcag ccacttatgt catagggggc
    11521 gtgaatctgc caatgttgtt agaagcaatc aatcatcaga tgcttcacac accgatagaa
    11581 gaagcggtgg aacaaattgt caaacaagga aaagataatt taactgtttg gcatgcttca
    11641 gcggaagaaa aaaaattaga tgaggacgat ttttagaagg aaggctgaaa agatgaagtg
    11701 gggatttaga tggtatggtg aaaaagggga ttcgatccca ttaaaatatg ttcgccaaat
    11761 accgggaata aatgggataa ttggaacttt gctgactaaa atgcctggag atgtttggga
    11821 gatttcagaa attgaggagt tgaaagcatc tgttgagagc caaggactgg aactgcttgg
    11881 aattgaaagt gtagcgatcc acgatgcaat caaggcaggg acagatgaac gagattacta
    11941 tattgataac tatatacgaa caatcaaaaa cttgtctcga tgtggggttc gattgatttg
    12001 ttatagcttc aaaccaattt ttggatgggc gaaaactgat ttgtttaatg aaaacaacga
    12061 tggaagctat tctcttttat acgatcagaa aatcgtcgat cagatggagc caggagaaat
    12121 gtatcaattg atacacagcc agtcaaaggg attcaaattg ccgggttggg aagaagaacg
    12181 gttgaaaaaa ttcgctcagc tggaaaaact gtatgaagga atgaccgaag aagaattatt
    12241 tgaaaatctg acttattttt tagaaaaagt gattccagtg tgcgaaaaag cagatgtaaa
    12301 aatggctatc catccagatg atccgccatg ggaacttttt ggttttccta gagtgacgaa
    12361 aaatcttggc gacttgaaga aaattctaaa tgcagtagat tctccatata atggactaac
    12421 gctgtgcaca ggatctttag gggcaaatcc ggaaaacgat ttagttgaaa tcatccatga
    12481 ggtaggttcg cgcatccatt ttgtccactt cagaaacgtg gagtttttag gagaaagaaa
    12541 gttcaaagaa acggcacatc cctctacaga aggctccctt gatatgtatc atattatgaa
    12601 agcgttggtg caagaagggt tcgacggtcc tatacgccct gatcatggta gaaccgtgtg
    12661 gggtgaagtg gctatgcctg gatatggcct gtatgatcgt gcaatgggac tcacttacat
    12721 gcaaggtcta tatgaggcga taagtaagga aaaaccactg ggagagcatt atgaataaaa
    12781 tcaagctctt catctttgat atggatggcc ttttattaga aactggacgt ttagcttatc
    12841 gtgcttatgt aaaatcagca caaaagtatg attatgaaat gcgtaaggaa gtctattatt
    12901 tactaactgg ccaaacagaa atggcgataa gagaacaaat gggcattttg tacggagaag
    12961 atgttccata tatcaaatgg cgagaagcaa tcaatcaata taaagaaaaa atcgtgaaag
    13021 aagataagag agtatatacg aaaaaaggag cagaagaaat tttatctttt gcaaaagaaa
    13081 gaggaatcca tacaatcgtg gcttcgtcta atacacgggt aaaagtagaa atgtatttaa
    13141 aaatggaaaa tctatatgcc ttgtttgatc aaatcatttc aggggatgat gtaaaaaaag
    13201 gaaagccgga accagagatc tttttgaaag cttgttcaaa aatgaatatt cctccaagtg
    13261 aagcacttgt atttgaagac tcgattgcag ggatcgaagc agcaaggcga gcgggcattt
    13321 tgtcttttct catcaaagat catttgatag ggttgcccga tcataatggg aagcataagt
    13381 tgaaagtaga tgtaagttta tatgaatcca ataaagcaga ctatgaattt tatgacctgc
    13441 atcaagcaaa acgattttta gaaagtaaag ggttgtatct ctaataaaga ggactatttt
    13501 ctagtcctct ttatttattg tcctgcacga ctgagggaac agatgaaaaa gaagtaattt
    13561 tttattttga agtatctacc ttatttgtag tgaacaattt agaacaaaca caaaacgcat
    13621 ctactgttat ctaccagtag atgcgtttaa agagaagaac gagaatcgtc catatgaggg
    13681 tttccactcc ttatgcagat ttttttaatt cttgcactgc ttgttcgatc gtaacacctg
    13741 tcgctacatg attggtattt tctttgtcaa aaacgacaaa tagattcaaa tctgtatcca
    13801 aggttactcg atattgtata tctttgttat agaatgtcat agccattctc ctttctttaa
    13861 atacttgctg aaaggcaacg aacgcgttga taacgaaatt ccatttataa gtataccacc
    13921 aattgaaagg ttttcatagt taagaaattt taaagtcaaa agttgcagat aaaagcaaat
    13981 aaacaagaaa acaaaaagtg aatcaatcgc ggttattcaa ctttttcttc gattcgaaga
    14041 caatagaaga caatgaattt tatatttcat tgcattatcc ttgtgttatt agaaaaagca
    14101 tgatatatta aatgagtaat ctattttgaa acgtaatttg agcgatatat ctacatagat
    14161 aaaactcctt ttgcctatca aatatttatt gcaacgaaaa aacgtacatt gatacgtatc
    14221 aggaggaatt tatttatgaa taacggtaca gtaaaatggt tcaacgcaga caaaggtttt
    14281 ggctttatca ctggtgaaga tggaaatgat gtatttgcac atttttcagc tatccaaggt
    14341 gacggcttca aaactttaga agaaggccaa gcagtgtctt acgatgttga agatggtcaa
    14401 cgtggccctc aagcaacaaa cattgttaaa gcttaataaa aattgacttt taaccgtctt
    14461 ttcgaaagaa aagacggttt ttttgtacga aaaaatt
//
LOCUS       DAYYME010000065        14332 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000065 DAYYME010000000
VERSION     DAYYME010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.066"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            81..1421
                     /locus_tag="ACHBFW_001675"
     CDS             81..1421
                     /locus_tag="ACHBFW_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9317980.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1639..2082
                     /locus_tag="ACHBFW_001676"
     CDS             1639..2082
                     /locus_tag="ACHBFW_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM9317981.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2586..4004)
                     /gene="nhaC"
                     /locus_tag="ACHBFW_001677"
     CDS             complement(2586..4004)
                     /gene="nhaC"
                     /locus_tag="ACHBFW_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM9317982.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4407..5630)
                     /locus_tag="ACHBFW_001678"
     CDS             complement(4407..5630)
                     /locus_tag="ACHBFW_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM9317983.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5661..6500)
                     /locus_tag="ACHBFW_001679"
     CDS             complement(5661..6500)
                     /locus_tag="ACHBFW_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM9317984.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6517..7197)
                     /locus_tag="ACHBFW_001680"
     CDS             complement(6517..7197)
                     /locus_tag="ACHBFW_001680"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM9317985.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7199..8272)
                     /locus_tag="ACHBFW_001681"
     CDS             complement(7199..8272)
                     /locus_tag="ACHBFW_001681"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM9317986.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8328..8566)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8609..8842)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8919..9590)
                     /locus_tag="ACHBFW_001682"
     CDS             complement(8919..9590)
                     /locus_tag="ACHBFW_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9317987.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9677..12325)
                     /gene="mgtA"
                     /locus_tag="ACHBFW_001683"
     CDS             complement(9677..12325)
                     /gene="mgtA"
                     /locus_tag="ACHBFW_001683"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFM9317988.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(12667..12841)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            13014..13451
                     /gene="ndk"
                     /locus_tag="ACHBFW_001684"
     CDS             13014..13451
                     /gene="ndk"
                     /locus_tag="ACHBFW_001684"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFM9317989.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13473..>14332)
                     /locus_tag="ACHBFW_001685"
     CDS             complement(13473..>14332)
                     /locus_tag="ACHBFW_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM9317990.1"
                     /translation="GELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSFVTL
                     RSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHFKKT
                     EDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFPWIF
                     SDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNEDMKR
                     SLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSLEKK
                     LLAIAEPSN"
BASE COUNT         4101 a         2995 c         2541 g         4695 t
ORIGIN      
        1 ttatcattag tcattgttaa atattgctat ttattcttaa tataaatttt gattgtgaat
       61 ttaaaagaaa gaggtcattt atggaagata aaaaattgaa aaaacaactt agctccagac
      121 atatcaccat gttagcttta ggtggagcaa tcggtgcagg tctatttaaa ggaagtggcg
      181 aagcgattgg gatcgccggc ccttctgtat tgattgcttt tttgattggt gggctgattt
      241 tatatatcgt gatgaaaggt ctgggaaaaa tcgttttaag cggtggcgac acgcatcacg
      301 gattatctgg tttgatacgc ccttatctag gtgctcattc tgctgatttt actgattggg
      361 tctactggtc tatgtggatt atcaatatca tcgctgaagc tgttgcagct gcttcctttt
      421 tgcagttgtg gttcccgcac attccagcct gggtatttgt ttttatgttg gctgttttaa
      481 ctacgatcat caatctttat tcggtacggt tattcgcaga aacagaatat tggctggctt
      541 ttgctaaaat cagtgtcatt atcttactga tcatttttgc tacttatctt gtcggtcagc
      601 aaatgttggg cacaggcgtt ttccctaccc ttcagcaatt gactgaccat ggtgggttta
      661 ctcctcatgg tatgaaggga atcatcagtt ctttgcttgt agttatctat tcttacggcg
      721 gttctgaact gattgctatc acggtaagcg aagcagatga tcctaaacgt gcgattccta
      781 aagcaatcaa aggtgtcatg ggaagaatca tttcctttta catcattcct ttgtttttgc
      841 ttttgattct ttttccttgg aatactttag caggcacgaa tgtcagtcct tttgtgatgg
      901 tatttgaaaa aatgaatatc ccatttgcag cggatatcgt caattttgtg attgtccttg
      961 ctttgttctc atctatcaat tcgggtgtgt atgcatcttc aagaattttg ttcttccgtt
     1021 tgaaggatcg aaaaggttcc tctagaaaac tagctgtttt aaacaagcat caagtaccgc
     1081 aacgtgctgt tttattttgt accagtgttc tttatcttgg tgtagctctt tcttattttg
     1141 tcggtgacaa attatttggc tatctggcag gttccctttc gtatacagta ttattgatct
     1201 ggatcattat cagtgcggca tcctttgtac tggcactaag aaaaggaaca ttatgggata
     1261 aaggaatcag cttttttgca ttagctgtct taggattgat tttccttggt atcctgttca
     1321 cgaactccat tggggtaact gtattgactg gcctgcttta cctgttcatt tatttcagtt
     1381 atcaaaaaaa gaatgatgcg ttcgtattga cgaatgaatg aaatcgatag aaaattttat
     1441 tttatttatg tggaatagat ggagctattg aagaaaggag ttgtgacaaa agtcttgttg
     1501 caactccttt ttccgaataa acagtctcat gacctcttgg taaacggtgt tcaaaaaccc
     1561 tctgtgcaaa ataattgatt gttctccatg ctcatgacat gatagaagaa aggaatgaaa
     1621 aaaaggagtt tgttttaaat gaaaaagtat tggaagtttt tgttgatgat cggtgtttca
     1681 acggtagtta tgtttggtat gatgtacttg aatgtctatg cgctcgatca tatcttcttc
     1741 agtcgtacac gagtattcat ggcgttgatg atgggtgcag tcatggcagt catcatgctg
     1801 ttgttcatgt ggaaaatgta tgaaaacaaa aagataaatg ttatgatctt agccgctagt
     1861 cttcttgttt ttgccggttc cttgtttatg gtacgaagcc aaactactgt caacgacaca
     1921 gcttggatga aggccatgat tcctcatcat tctatcgcta tcctggcaag taaacgagct
     1981 gatttaaaag atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa
     2041 gagatcgaca aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc cgtccaaata
     2101 aaaaaagagg atgttccaga agttatcagc ttcaagaaat aagaagaagc tacttctgga
     2161 acgccgtgaa taaggggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa
     2221 caaaaatagc ttctcctatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt
     2281 gaacaccgtt tattaagagg ttgtaaaatc agcgttctga cctgagtatt agagagacaa
     2341 atggaaaaat agttcctcat attttgccat tttttgagct aataccgagg gtcagctgat
     2401 tgaacaccgt ttatcgagag gttgtgaaaa agtcgcttag tttcgcgtat taagaaaaat
     2461 tttgtgaaat ggctttccac atttttgtat aaatttggct taataccgag aaactggctt
     2521 ttgaataccg tttattcgta aaagggattg tgacaaaagt cttgtcacaa tcccttttta
     2581 tgtttttatt gttctaattg cgctgtcttg cttactgctt cttgcggttt cacaaatttt
     2641 tttgagattc cccaacctgt gaaggcactg accatcgaaa acacaggaga aagcaaactg
     2701 aaaaatacga atggcaagta agtgagtgta ggaataccaa atgtacttgc tacgaaactt
     2761 ccggcaattc cccaaggcac taagtagttg attactgttc caccatcttc tagcgttcta
     2821 cctaatacag ttggatcaaa tcctgctttt tcataagttt ctttaaatgc attaccaggc
     2881 agaataacag ataggaattg ttcaccaata aaaatattta ctccgatact tgtcatcaat
     2941 gttgtaaaaa tcaaatttgg tgtgttcgtc atctttcttg atactcgttc catcacggtt
     3001 gagatcaagc caaattcgat caacaatcca cctaatgaca aagttagaat aatcaatgac
     3061 acagtaggca tcatactttc gattcctccg cggctaagca gtgaatcgat ttgttggttt
     3121 ccagtatttg aaacaaaccc tgattcgatg accccagcca acttcgttaa ggaagtttgt
     3181 gggttttgaa taaaaatcat tcctactgct acagcgatat tcaacaaaat cgttaaaatt
     3241 gccggcacct ttttccatgc acacagtaac attaaaaata aaggaatcag tgaccagatt
     3301 gaaatagtaa agtttgcttc tagaacatca gtcacttcac gaatagatga aagacttgcg
     3361 gctttacttg tgtgtcctaa aatcatgaat aaaaagaaag aaacaacaaa tgccgggaca
     3421 gttgaccaca tcatatgctt gatgtgtttg aacaaatcgg catcaacaat agctgctgct
     3481 agatttgttg attcggataa tggagacatc ttatctccaa aaacggcacc tgaaacaata
     3541 gctccaacaa ccagcgctgg atttaagcct agtgttgtcc caataccgaa aaatgcaatc
     3601 ccaatcgttg acataacagt aaaggcactc cctacagcgc tacctacgat tgcacacaca
     3661 acaaatacag aagggacaaa ccactggaca ctgatcaatt tgaacccaat gaccattaga
     3721 gttggaatga tccctgcttg tatccataca gcaatcaatg aaccgactaa aataaaaata
     3781 aagattggga tgattcctgg tttgatgcca ttgatgattc cttcgtgcag ccaatctgtc
     3841 gggtatcttt ttatcactgc atacaacatg accattccga tcgctgctaa gatcgtgata
     3901 ttaggtgaga cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat aaccattaat
     3961 acaagtgttg cttctaaaaa acttggtgtt cctttttttc ccatgataat ctctcctttt
     4021 ttagttgttc ttatgttcta aaaaagttaa gatatcggat tactccgccg caattcatcc
     4081 gcgattcctc ctttataaaa ataaaaagac ctgttttaaa ctaaggacga atgactccgc
     4141 ggtaccacct taattaaaaa cagatctgtt tttcactttc aaacttttat tcaaatgtat
     4201 ggactttcca tgtaattcgt caattaggct tgatcggttc acagcgacca ccgactttct
     4261 gaacaaaaca cctaaccgct actgcgtgaa aagttgactt gtacgaagtt taccttccat
     4321 aagatcattt gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat tatttttata
     4381 actttcacac acaagtttat tttttactag aaagtagcgc cctcttgttt ttttcttgct
     4441 actatttctt gatgttcttg cttgattctt tctaatagct ttggctcttt caacagatca
     4501 agagctgtat tggcgagtag ttctgctgca atcgcaatag aagcaaggcc tttgtcactt
     4561 ttagcagcag ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga gattgctacc
     4621 gtaggctgga ttgttggtat gacttggctg acatttccta catcagaaga accgagactt
     4681 gttctttgtt ccgtttctat ttcttcatct ggaacacctg cttcttttgc atgctggaag
     4741 aataagtcat caaaagaagg agtaacgatg acatcatcaa ctgcattttg gaacaatcca
     4801 aactcaaaag tcgtgcccgt acttatcgca gcacctcgta cgatattttc cactttttca
     4861 tatactttat ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc agcagcgtaa
     4921 tctggaatga cgttggcagc tactcctccg tctgtaatga tcccatggat attgacatct
     4981 tttggcaatt gcaagcgtag agcgttgatt ccattaaaca cttggatcaa tgcttccaaa
     5041 gcatttactc ctttttcagg tgctgctgct gcatgggaag cgacaccgta aaatttgatg
     5101 tcgactggat cattcgctaa agattctgtc gttttcccat aacgaaaagc tggatggacg
     5161 cataacgcta catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc tttagctgat
     5221 ccgttctcgc ccccttcttc ccctggggtg ccatatacac ggatttctcc accagtttca
     5281 tcaatgaatt gcttcactat gctggcggct aatacggagt aagtaccaaa caaattatgc
     5341 ccgcatgcat gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc aattgtggga
     5401 ccgggtttat tggaaatata acgtgcatca aaggctgttc ggtgtccagc tacttctttt
     5461 tctacccaaa acctttcttt ttctaattgt ttaatcaacg ctgctgagga ataaaactca
     5521 tagttactta cttcaggatg ttcgtggata tctagagcaa gtgcttgata ttctggtaaa
     5581 cgatattgga ttagatcctt caattggttt gttgagattg ttgttgtcat aaaaaattcc
     5641 tcctatgcta gtcatttttc ttattttctc ccgaatgttt cccatgcagg cacgcttgat
     5701 ccttttgatg ttttctcgat tactttgact gtgtcttctg tttgataagc gtctactatt
     5761 ttttgatacg ttttattgtt ttcatcttct tttcttgcaa cgatgatatt cacataagga
     5821 cgtgcatggt catccactgg ttctaagaag atggcatcag aagcaggatt aaatccagca
     5881 tctacagcta caccgctatt gatcactgaa atgtctacat ctgacaaagc acgtgctgtt
     5941 tgggaagcat ctaattcaga aatgtctaga ttttttctat tctcggtaat atcagaaaca
     6001 gtgggcgctt gtccttttgc cttatccact ttgatcaatc cagcagtttg caataacaga
     6061 agagctctgc cgccatttgt aacatcatta gggattgcta cttttgctcc gtctttgata
     6121 tcagctacat ccttcaaagt gttagaataa attcccagcg gtgcattgac agtatttccg
     6181 atcgatacta aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa ttgatgttgg
     6241 aaagaattca aatcaatcga tccatctgct aaagccacat ttggttgatt gtaatctgta
     6301 aatttcacgt attccaaatt aatgttctcc tttttcaaac ggtctttcac gttgtcccat
     6361 acatctgtat cttctccaat aatccctagt tttacgatag tttctttttt gtcagttgta
     6421 tctgcttttc cttggctaca ccctgccaac caaataattg ctacaaatgt aatgactaca
     6481 aaacgaataa tttttttcat tttttctcct ccactttcaa tgtgtaattt ttttaatcag
     6541 ttgattactg ataaattgac ttaaaaaaac taagattaga atcaataatg tagccacaat
     6601 cgttacatct gtttggaatc gattataccc tctcgatata gcaagattcc ctaatccgcc
     6661 tgcacctact gctccagcca tggtagtcag tccgatcacg ttgatgatag tcaacgccga
     6721 gacccgaatg ataccaggca aaccttctaa caaatacaca cgtaaaatga taccgattgg
     6781 actggttccc atcgcttctg ccgcttcgat caccccagga tcaacttcta gcagtgcgtt
     6841 ctcgatttga cgagcaaaaa acggaacaac tccaaaaatc aatggcacta aagctgcttt
     6901 cgcaccaatc gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag aaagcaagat
     6961 aatgaaaggg atggaacgaa tgatattgat cagcttatca agtatttcaa atagcagacg
     7021 gttagctagt aatccattag ggcgtgtcac tactaacgtc acacctaaga tgattccaat
     7081 catcccgcca atgaatgctg tccaaaaaga catatatagt gtttgcttcg tcgcttcgac
     7141 aaattcatcc ggaatcaaat aaacattggg caaatatgtt ttaaagaaat cagtcatttt
     7201 atcttcctcc ttcgatcact ttgatacgac tatctaaatc gtttttttgt aaaatattta
     7261 tttttacacc agatttgatc aaataatcca atgctttttt ccgctgtttg gtttctccag
     7321 ctaaagtaac gatcaaactc cctaacggcg tttcttgtaa taactctaca ttgccatata
     7381 aaatattcgc tgttacttga tatttgctgt acagatgagc gattagaggt tcgttcgtct
     7441 gatttccaat ataacttaat tccaccagcc atttattggc gatctgatcg gcaaaagcag
     7501 aatgggcaat gatcgtttct aatgcttggt caagatgagt agctgttcga ataaaatctt
     7561 tggtcaattc ttcttcagga ttgctgaaga tctggatgct gctacctttt tcaacgattc
     7621 ttccatcttc cataaccgct accttgttgc agatttcttt gacaacttgc atctcatgag
     7681 tgatcaggac gattgttaaa cctaactggc gattcaattt ttttaacaat gcaaggatct
     7741 gaagggtcgt tttcggatca agtgcactgg ttgcttcatc acataataat accttaggtt
     7801 cattcgccaa agctcgtgcg attgctactc gttgtttttg tccccccgac agttgtttag
     7861 gataagcaga tgctttttct tccaaaccga caagttccag tagttcattc actttttgac
     7921 gacgttcctg tttcgatttt ccagaatact ttaaagaaaa atcaacgttg tcaaaaatag
     7981 ttcggctttc cattagattg aaatgctgaa agatcatacc gatggccttt ctttcttttc
     8041 gcaattcttt gggagagagc tgactcagat tcgtctgatt gacaatgact tctcctgctg
     8101 ttggtttctg cagcaaattc atcacacgaa ctaacgtact tttccctgca cctgagtaac
     8161 cgacgattcc ataaacatcg cctttttcaa tcgttaaatc tacagaatct actgcctgta
     8221 tctctttttc tttttgtttg aaacatacag aaacatctcg caattcaatc attaaaattc
     8281 ctccttatct aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt gaggacgctt
     8341 ttttgcgtgt taccacctca tgttcaagct gtgttcacac acaactcctc ttcaagtacg
     8401 atcatactct agcaagatat cggtcgcagc cgtcatcgcc ttacatctat gctcgggatg
     8461 aattgctcga tggccatttt ccaatagtcg ttccttgctt cttctcagct accgaagctc
     8521 tctgtcaaag gttcttctat cgtacttacc atttcaatgc aaataatatt caaaaaaaaa
     8581 gtccttttac taacataaac tatgtcagta aaaggacgac gtatcgcggt accacctttt
     8641 ttcggaagtt tccttccctc aagcccaaca tctcaaaaag agagattggc gttcgtttta
     8701 acggtcgaac agttccgaat ttgcctactt atcgacaaat tattctccaa ggccattttc
     8761 agcttttctt acttgactct tctcaccagc cagagcctct ctgcaaagtt tccaaactta
     8821 ctttcctttt caacgaaatc gatattttgt tgttgggatt aatctacatg aggaaaactg
     8881 aaaagtcaag tacgaaattc aaattatttt ttctatattt atttttctgt ttttttattt
     8941 aacatttcaa taacagttcg aagatctttt ccttccgcaa gaagtttcaa tttttccttt
     9001 tcttcgacgg tataatttcg gacaaaactg gaatcaggta tcaaaacaac gcctaatcct
     9061 gctcgtgaag cagctgttgc tcctactaaa gaatcttcca ctgcaaaggc tgaactagct
     9121 tctaatcctg ttcgttccaa agctgtcaga taaggaatcg gtgaaggctt gttctccgac
     9181 acttcgtctc cgaatgtaaa cgtgtccaag taatcagcta atttaaagtg atccaagata
     9241 tctgttgctc ttttcgtaac agtggtcgta gcaatgccta gtttcatatg acgttctttt
     9301 aagtctgcca atatttcttt tgcatatggt tttaagtgga tggctccgct tgtcagctgt
     9361 tgataaaaat attcttcacg cttcgctcgg acttcctgta ctttttcagg gctacctgct
     9421 gctttcacta aaatatcaaa actttgtttc cagttttttc cgccccaagc gtcaacgatt
     9481 tcttttggga tatggatatc aaattgctga aatccccaaa gccagccatc acgatacgtt
     9541 ttttctgtgt caactaacaa accatctaaa tcaaatattg cacctttcat ctatacatta
     9601 atcctttcca aaacaataca catagtttag cacgactggt ccaaaaagaa cagaagcaaa
     9661 gaaccagcct tctcgtttat aagagttctt ggaatcgacg gatatagatt ttcttcacag
     9721 ctgtcacaag cgccagataa gcgatgactg tagcaaataa ccataaccag taattgccag
     9781 gaagtggcat caagcctaat tcagcaccaa aacttgtaaa aggaaggaca gaaccgacaa
     9841 taattcctaa tgtagtgata acaaacatgg caaatgttgc gttgctttgg ataaatggta
     9901 ctttcggtgt acgcagtgca tggagcacta aggtttgtgt ccatagtgat tcgacaaacc
     9961 atcctgcatg gaataatgcg atgaaaatca ctcgctgtga actatccaat gtatggaaac
    10021 ttccgcccac tacagcagga caaataacaa aatacatcaa caagtaagta gtaatatcaa
    10081 atatcgaact tgttggacca aagtaaacca tgaattcccc aatactagat gcattccatt
    10141 tttttggctc gtccaaatat tctttatcca tccgatccca tggcaaggaa atgcaggaaa
    10201 catcatagat cagattcaaa aacaaaagtt gtaaaggcag cattggtaaa aatggcagga
    10261 acgtacttgc aatcagcacg gaaaacatat tgccaaaatt agaacttgct gtagccttga
    10321 tatatttcat gatattccca aaagtttctc gtccagaaag aaggcctctt tccaaaatcg
    10381 tcaaatcttt ttctaaaagg atcacgtctg cagattcttt cgcaatatcc acagctgtat
    10441 ctacagaaat accgatatca gccactttca ttgcaggagc gtcattgata ccatctccta
    10501 aaaatccgac agtatgacca tttttacgta aagctgtcgt caaacgtgct ttttgctgag
    10561 gattcaattt tacgaatact tcatggtctt cagctacttg tgctaattca tgatcactca
    10621 tttccgagat tttctcaccg gtaaccaatt cattgatctc tagacctact tgacggcaca
    10681 ctgaacgtgt aactaattcg ttgtccccag tcagaacttt tacagctaca ccatgttctt
    10741 tcagagcttt caacgcttgt ttcgttgttt cttttggtgg atcaaggaaa gcaagataac
    10801 caatcaaaac catgtctgac tcatctttga ctgaaaattc tccaacgacg cttggattgg
    10861 tcttttgtgc gacaccaatc actcgtaagc catcttcatt aagtgactgt acttttgcaa
    10921 gtacggcttc ttttcgttct tttgttaatg gtgtaacagt accgccaaga tcaataaatt
    10981 tagaaatgtt cagcatttct tcaatcgcgc cttttgtgat catttgtgtt ttaccagctg
    11041 gatcagcaac cacaacactc atacgacgac gttcaaaatc aaaaggaatt tcatccactt
    11101 tttgataatt gatcttatct gtatctaaag tttctgttgc ttcatcaatg atagccacat
    11161 ctaacaaatt tttcagccct gtttgatagt agctatttag atacgcatgg cgcaaaacac
    11221 gatcatcttc atttccttca caatccatat gatattcaag aatgatttta tcttgtgtca
    11281 atgtccccgt tttatccgta cataacacat cgattgcacc aaagttctgg atggcattca
    11341 aatttttgat cactgttcct tttttggcca tcacacttgc tcctttgacc aaattcgtcg
    11401 taacgatcat tggaagcatt tcaggtgtta gaccaactgc tacagatagt ccaaaaagaa
    11461 aagcttctag ccaatcccct tttgtaagcc cattgatgac gataactgct ggagccatca
    11521 atgacataaa tttgatcagt agcatggatg ttttatggat gcctaattca aagcttgatt
    11581 gaattggttt ttcagatagc gtttctgcta catgaccaaa tagtgtttca tttccaacag
    11641 aaacaatgat tccttccgca cttccactga tgacattact tcccataaag acaagatttt
    11701 cgtaacttgt ttcgctttgc tgatcgtctt cacatacttt cgcatgcttt tctactggat
    11761 agctttcccc tgtcaatgct gcttgggaaa tgaataaatc ctttgcggac aataaacgca
    11821 tatccgctgg tatcatatcc cctgctgaaa gccgaacaag atctccacag acgatttctt
    11881 ccataggaat ctctgtgtac tttccattac gttttacagc tgccgttact ttcacaagtg
    11941 atttcagttt ttcagccgct tgattggaac gtacggactg aatcaacgtc attgtcccgc
    12001 tgacgataac catagtgaag ataatggcta caccgaaaag atctttatct ccaggagctg
    12061 ctaatacata ttccgtgatg aatgaaataa ttcctaatgc gatcaacact aatgtaaatg
    12121 gcgtgatgta tgctttcaat atttctacaa tgaatggtgt ttttttgcca tatgaaatcg
    12181 cattttcacc gtaattttct cttgaacgtt cgatttgttc ttctgtcaat ccatttgctg
    12241 atgcatggaa tagttcaaat aattcttgtt tcgtttttcc agcaaaagct gcatagattt
    12301 tttgtgtatc tttctttttt tccatctcaa tctctccttt gtgtccaaaa gacagctgtt
    12361 ctcgctgctt ttggtcttta gctattcatc ggatcgatag agaaaaaact gaaaggggct
    12421 gagaacagat agataatagc cctggccttt cctctagatc cttgtttttc gaatgactgt
    12481 catccatttg ttctcatctc ctttactaag taattttaat tcatgacttc caatcatttt
    12541 tcgaatagtt atttttccaa ttagaccacc tcctttttat ttttcaccga tggggatttg
    12601 cggcaacaaa aaaacctctc gccaaatact cagcgaaagg tttgaaaaag catattaaaa
    12661 aaagcttctc agatctcatc gttgagtttt gacactgtac aacgtaaaaa gcagatacct
    12721 gcttttagct accttatgaa aagccttatc tcgacaattc ctgttctacc cattggcgtc
    12781 tctcgacgtt tttgggcagt agcctatgtt tgtataggag cctcacctaa caaatcttat
    12841 tcggttttcc ttcgttaata taacaactaa aaaagaggat gtcaaatcaa ttttccatgt
    12901 ttttcatact ttttttaaaa tacacacgtt atattcctgt ctcacgcggt tttatttgtc
    12961 tcattttcca cctcccgcta taatagaaat atagtaggaa acgaggtgct gttatggaaa
    13021 gaacacttgt tattatcaag ccagacggcg tgagacgcca tttagtcgga agcatcatcc
    13081 aacgttttga agcaaaggga ctggccattg ctgaaatgaa gtttgaaact atgactcctg
    13141 aattagcaaa agaacattat cagcacttga ctgagcgcag tttttttgat gaactgatcg
    13201 attacatgac ttccggtcct gtcgtttatc ttgtgctggt cggcgaggaa gtgatcgata
    13261 tcgtccgtaa gatggttgga gcaacaaaag ctgccgatgc tgttccaggt acgatccgag
    13321 gagactacgc gctgccgggg actgaaaaca tcattcacgc ttctgattcc cgtgatgctg
    13381 cggtaaaaga gatcgcccgc tttttcccgc cattcgatat aaacaaaccg tctgaaaaag
    13441 cggcgaagtg atacaaattc taaactgctg gtctagttgc tgggttcagc aatggccagc
    13501 agttttttct ctaaggacct ccgttgattc tttcctaaca tatattcgag cactgtgaag
    13561 aattcttggt agtctttcgt cttttcttcc cctaaagcag cagaaatcaa tctttgataa
    13621 taatctgcca tcaatgtttc atagatcact gtacttcttt ctttatgtga taagctccgt
    13681 ttcatatctt cattcacact acgtgccttt ttcaaccgac cattttctat ataaaacgaa
    13741 gtcaggttca ttaaaaaagt aaagagatac tgctcatgat tgttcaactg tgctaatcgt
    13801 cgcattcttt ttttgatttt caaataattg ttctcaatga accgatcact gaatatccaa
    13861 ggaaaatcag ctagaataga tagttcaaat tgaccatagt ctggtatctg catcaagtat
    13921 tcttcaatga ttcggcattc ataacgtaaa aattcaaatt ctgtctcaga tacttctgtt
    13981 ttttcgacca tgtgaagatt caaaaccaaa taatacagat cttctgtttt cttaaaatgc
    14041 ctacggttat tgctgatctc tttataagga tcaaagctgt cttcttgtac ccgatctagc
    14101 attcgtcttt ttttctttaa ctttattggt tcatccagct ggtttcgcat ccattcatat
    14161 tctgcaagtg tcatgttcat tcgctctaaa aatgatctca atgttacaaa tgacattttt
    14221 ttcactgaat ttgtctcaat cccgactaga gaagtcttaa aacagattcc ttccgctaat
    14281 tgggactgtg tcaacttctt gttgactcga agtttcttga tcaattcacc at
//
LOCUS       DAYYME010000066        14257 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000066 DAYYME010000000
VERSION     DAYYME010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.067"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(433..1617)
                     /locus_tag="ACHBFW_001686"
     CDS             complement(433..1617)
                     /locus_tag="ACHBFW_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFM9317991.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(1642..2649)
                     /locus_tag="ACHBFW_001687"
     CDS             complement(1642..2649)
                     /locus_tag="ACHBFW_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9317992.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(2821..3180)
                     /gene="comGG"
                     /locus_tag="ACHBFW_001688"
     CDS             complement(2821..3180)
                     /gene="comGG"
                     /locus_tag="ACHBFW_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFM9317993.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(3152..3577)
                     /gene="comGF"
                     /locus_tag="ACHBFW_001689"
     CDS             complement(3152..3577)
                     /gene="comGF"
                     /locus_tag="ACHBFW_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFM9317994.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(3540..3887)
                     /locus_tag="ACHBFW_001690"
     CDS             complement(3540..3887)
                     /locus_tag="ACHBFW_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9317995.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(3859..4329)
                     /gene="comGD"
                     /locus_tag="ACHBFW_001691"
     CDS             complement(3859..4329)
                     /gene="comGD"
                     /locus_tag="ACHBFW_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFM9317996.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(4304..4582)
                     /gene="comGC"
                     /locus_tag="ACHBFW_001692"
     CDS             complement(4304..4582)
                     /gene="comGC"
                     /locus_tag="ACHBFW_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFM9317997.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(4579..5628)
                     /gene="comGB"
                     /locus_tag="ACHBFW_001693"
     CDS             complement(4579..5628)
                     /gene="comGB"
                     /locus_tag="ACHBFW_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFM9317998.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(5585..6514)
                     /gene="comGA"
                     /locus_tag="ACHBFW_001694"
     CDS             complement(5585..6514)
                     /gene="comGA"
                     /locus_tag="ACHBFW_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFM9317999.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            6646..8400
                     /gene="ade"
                     /locus_tag="ACHBFW_001695"
     CDS             6646..8400
                     /gene="ade"
                     /locus_tag="ACHBFW_001695"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFM9318000.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(8498..9160)
                     /gene="trmB"
                     /locus_tag="ACHBFW_001696"
     CDS             complement(8498..9160)
                     /gene="trmB"
                     /locus_tag="ACHBFW_001696"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFM9318001.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(9230..10012)
                     /locus_tag="ACHBFW_001697"
     CDS             complement(9230..10012)
                     /locus_tag="ACHBFW_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFM9318002.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(10216..11427)
                     /locus_tag="ACHBFW_001698"
     CDS             complement(10216..11427)
                     /locus_tag="ACHBFW_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9318003.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(11420..12157)
                     /locus_tag="ACHBFW_001699"
     CDS             complement(11420..12157)
                     /locus_tag="ACHBFW_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9318004.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            12295..12729
                     /locus_tag="ACHBFW_001700"
     CDS             12295..12729
                     /locus_tag="ACHBFW_001700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFM9318005.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            12722..13066
                     /locus_tag="ACHBFW_001701"
     CDS             12722..13066
                     /locus_tag="ACHBFW_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM9318006.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            13246..14256
                     /locus_tag="ACHBFW_001702"
     CDS             13246..14256
                     /locus_tag="ACHBFW_001702"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9318007.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         4533 a         2910 c         2401 g         4413 t
ORIGIN      
        1 ttttttcgca acaaaaaact tgattttcat acctgtgctt tcacgattca ggcaaagtac
       61 aggtggaaaa tcaagttttt attattcgat caatgaatgt ttcggatcgg ttttctagct
      121 aaacataaag cgaagaataa agtcgacagc ccacgaggct gatctaaaaa tgttttttct
      181 ttttacatat gtaatgtccc ttataaagat gaatgaaaat aaaatattgg gattttcctg
      241 aaaatcaata aataaaatgc cagtcttttc ccttttgacc acttttccaa gcaaaattcc
      301 ctaagaagat cctgtaaaaa agaagggaaa ttgaagttaa attaccattt tgcggtcttc
      361 tccgcaaaaa aaacacatgc aagaattccc tcttgcatgc gtttcatctt ttatcctaac
      421 gaatgctttg gcttagccgc gcagacgctc cacatcacgg gcaatcatta gttcctcatc
      481 tgttgggatc actaatactc ttacttttga ttcttttgta gagatatcta cttcttcacc
      541 acgcaattca ttcaacgctg gatcgatttc acaaccaaac caagacatac ctttgatgat
      601 ttcactacgt acatgtccgt cattctcgcc gataccagca gtaaagacaa ttgcatcaac
      661 accattcatc gttgtcacat aaccgccgat gtattttcgg atgcggtcca cgaaaatgtc
      721 ataagctaaa cgaatatgtt ctttttcata gttcttttca agatcacgca tatcactaga
      781 caatcctgtt aggcctagca gaccagattt cttgttcaaa acattgatca tttcatcaat
      841 atcaatctct aatttttcca ttaaatatgg gagaacagct ggatcgatat cacctgaacg
      901 tgtacccatt gtcaccccag ctaatggtgt gaatcccata gaagtatcca tggatttacc
      961 tccatcgact gctgtgatcg atacaccatt tcctaagtgg caagtgatga tttttagttc
     1021 ttcgatcggt ttgttcaata attttgccgc acgttccgac acataacgat gacttgtgcc
     1081 atggaagcca tattttctta ctttgaaatc ttcataataa gacaccggta agctatataa
     1141 ataattttgc tttggcattg ttgagtggaa agatgtatcg aatacagcca cactcaaaat
     1201 gtcaggtaaa atctttttaa acgctttgat ccccattaag tttgcaggat tatgcaaagg
     1261 agcaaattct gctaattcag caatgcggtc cattactttc tcatcgatca ctactgaatc
     1321 cccataagtt tctcctccgt gaacaacacg gtgccctact ccggtgattt catcataaga
     1381 gcttaaaatt tgcagttcaa tcaactgatc taacagcatt tttactgcta cttcgtggtt
     1441 ttcgatatcc atgatttgtt cgaatttttg attatcgccg tacttgatgg taaaaataga
     1501 atcttgtaaa ccgatacgtt cgacgatccc tttcgcaact acggtttctt ctggcatttg
     1561 atataattgc catttcaaac ttgaactccc agcattgatt gcaattgttt tagacatatg
     1621 actctccttc tcgttgaatg attacagagc agatgatttc cagtttttaa actcgttgaa
     1681 aaattccgtc accttctctg gttcttttaa tgaagcaagt ttcacaagca agacttcttt
     1741 tacttgttgt gcttgtggtc cttttttctg taagatcaaa atactttttt gcgattgttt
     1801 attacggaac aattcatctg gcaattgaat cattccttga agataacctt cttctttaaa
     1861 ccattttttg atttcttcac tttgatccgt ttctaaaaat ccactcggca tcaaaaacaa
     1921 accaaaacca tccggcttca catatttcat tgattgctct agcaataaat gatgtgcata
     1981 actatggcct tcttcggtac ttgttaaaaa ctcttttgct ttttcgtcat tcggataata
     2041 accgattggc aaatcgctga ttgcaaaatc gacaggatct attaataatt cctgcaaccc
     2101 gtcttgatga aagtattgaa catttgcttg tgtcaaatca cttgtagaag ctgcgacagc
     2161 caacaatgta tcatcaatat ccacaccaat tccttctgtt tggtaccccg cattcgatag
     2221 gtttaaaaga actgttaata acaagttgcc cattcctgcc gcaatatcca gaatcttaac
     2281 cggtgctttt ttatttgtgt atagttgttc aataagaaaa acaaataaaa aaccgatact
     2341 atcaggagtg agttgatggt tcgcctgcaa atgttctgtc tggctgccct tcaataaaag
     2401 taattgagac agtctgcgcc attcttctgg ctcgaaagaa agtttcttca actcttcata
     2461 aagagctgtg atccgttgcg ttgtctcttt tgtcggcact ccatccacca cacgtacttg
     2521 ataatcatct attaaatttt ctgcattttc gacataggct tctaaaaagg aagttcccaa
     2581 tgcattctgt aaaagctgaa tagcttccag gttttgatta aacccttgtt ccattttttc
     2641 tggtaacatg gtgcacctca tttctagata tattataaca attttttcac ctgtttaatt
     2701 ctatcgaaca attgacaaaa tttcaatcgt tttcactgtt ttttatttgt gaaacggcaa
     2761 acattttttt attaaaaaca gtgttataac agaaaagagg gcgttttcac gaacattcca
     2821 tcattctgta ctagtatctg tatcattttt tacttcttct gtgaaccgaa agataaacgg
     2881 tgagacttcc acctctaaga atagggactg ttgtacccac tgatatgtta cagatccagt
     2941 tgtgtagtaa aaaactcctt ccgaactttc ggcaagcgta gactgttccg ataaagccat
     3001 ctgcttcatt atttttgcta tataataatt tttgattcgt atattaagat ccattgacaa
     3061 acggtagctt tctaatgctg ccatcaataa aaacgtacat aaaagaaata aaagcaagac
     3121 agaaaataaa atagagccct tataacattt tctactgatc gttttttgtc catatggcat
     3181 atccatcttc tccattttca aagtgtacat aaaaagaaat tgtgtgttct ttttccaata
     3241 attgaagctc agtgatactt gttaacatag gttgatgtcc gccggaagtt cttcttttac
     3301 gtatcattgc ctgatatctt tcgatgatat actgtttatc ctcgatcgtg tagtgcagtt
     3361 gctgattttt cacagagaca ttgtgactgc tcttgagttc ttcttctaat tgtgctaaaa
     3421 atattagcca ctctttttga tctttacgct gcaaaaattc acttacttgg atggtttgct
     3481 gaatcaaaag attgaatgtg agcaatagac tgctcaatat catcagtcca attagacatt
     3541 caatcagcgt aaatgctgga attacttgtt ttttcaatac tgatcgcttc tttctccata
     3601 ttgacaaaca ctttttcgat tactccatta ttatcgacaa tttggatatc gtatgggatc
     3661 atttcttccc ttcgctctgg tcttttgtga tacatccgct gcgtcttgat atcttcataa
     3721 atcattcgaa taaatagcag ttcctcccca cttttctttt ctgcctgaag caaaaacagc
     3781 tggccaaagc tcactaagta gacacagaca gaagcgatca taagcgctgt aagggactcc
     3841 aacaaaacaa atcctgtttt attcaattct ttttgtatag cgaccactcc ctatctggaa
     3901 ttgatacgta atcgtccgct ctttttcttc ccaataaaat tgataagctt ttagtgaact
     3961 ttcattcccg gttctagcag caaaagtaat agaagcatgg cgtttcaagg taatctcatc
     4021 aggaatatct aatacttccc aatttatttt atctggattc ggaacatgaa aaatgatttg
     4081 attctcttca ctattccaat aaaagcctgt ttggatctga ttcacaatag cgattttttg
     4141 cgtagaatag attcgttttt caaattggcc gaaaaattga tagactgcca attcattctt
     4201 ccatgcagaa aatgcgagaa ctggaaaaga aaggatcgtt gtaagtaata aaatcagtaa
     4261 gagtgattca aacaacgtat atcctgatgc taaaacctta ccttcaaggc cgctgataga
     4321 tttctgcatg ttctcgtttg atatagcctt cattcaacaa ttcctccaca gtaggaactc
     4381 ttccattatt ttgaagcgca tagagctctt tttgtgtgtc taccaactgg atgatcgctt
     4441 cacgactttc ttggtctaca tgattcctat aacgcgacaa attagggacg aacaacaaca
     4501 ctaatacact gatgatcagt aagacgatta gcatttcgat caatgtaaaa ccgctataat
     4561 aagatatttt tctctttttc atggcaaaac ctcctctatg cttccgtaaa caggtaataa
     4621 aacagcggca taaactaata aaatcaaagc cgctacgccc aaaaaaatga ttggctgaat
     4681 aaaatgcatc cagtgatgaa gacgtttgaa aagctgtttt cttgtccatt cgctgtaaaa
     4741 aatcagttct ttacctaaac ttccctttgc ctctccctgt aaaacaattt tactgaattc
     4801 ttctgataga aaacgataat tctggaactg ctcagccaat gacatacctg cctccaatcc
     4861 ttgaatcaac ttagaggcaa gatgctgaat cagactttct tgtttcgttt cctttaagca
     4921 gactatgatt tgccttaatt cgaaaccttc atgaaaaagc tttccaatct ctagtgacaa
     4981 ataagccgat tgatacaagg aatatatcgg tccaatcaat aattgtctgc ttatccattc
     5041 actctgatca acaggagaca acttcatcag acgatacctt actagtaaag ccaatagagt
     5101 aaccgctagg atgaatagta gaacatacca atgaaagact tgtatcagct gaatacccca
     5161 atgctcttgc atgaccattc ctgaaccaat aagctgaggc aaaacgaaca accgcatact
     5221 aaacaagata cctagcaaaa aggtgagcaa tagcatcgga tatgcaagca gctttttcaa
     5281 atcattgcga aatccttcaa caagccgaaa ctgtttggca atttccaaca aagttgtcgg
     5341 cagattccca tgacgttcag ctaactcgat ctgtaccagt tgctctgctc catatcctat
     5401 ttgcgaaagt acctcactaa acgcttgccc ttcctttagc tgatgttgga tatttgccaa
     5461 aataacaggt ggaaacgaac ggatcgtgag taataaataa agggattctt gcagactaaa
     5521 accagataaa agcagttctc ccatggtttg tgcaaactgg ttttgctggc ttcttgttaa
     5581 tcctttagaa tagttctgac tgccaaatat tttcctgagc ttcttcataa ctttcctccc
     5641 atgatttttt tactgattct tcctccataa attgataact ccataaaaca gaaggatggt
     5701 gattggcatc ttccaaaagt tcttgataaa tgacaccagc taaacattca gatagctctt
     5761 cttttttctt tgtcagttca tggattctga ccaatgtacc ttcaagattg cgtgcatgta
     5821 cggttgcaaa aacccggtgc cctgttagtg ctgctcttac cgcagcatgt gcagttgatt
     5881 catctcttat ctcgccaatg attagcaagt ctggacgatg ccgtaatgcg agtctcaaaa
     5941 gtgtatcata agtctgtgct attttcggat tgatctgtag ctgcagaaaa aacggttctt
     6001 ctatctctac aggatcttcg atagtaatca cttgcaattt ttcttccaaa gccaaccgat
     6061 acattagaga agtcttgcct gatccgactg gtccgctgaa aaggtataat ccacgtctga
     6121 cggttttctg taaaatagtt gcaaaatctc gagaactaaa gcaacgatgg ggtgtttcaa
     6181 aagtaccgta taatagccga atcactaaac tctctcgttg aaggtaatct cctacagaag
     6241 acaaccgcag acgaacttcc tgtttttcta aagaataagt aaatgcacct aactgagctt
     6301 ttcttttttc tccgatatcc atctgtccca aatacttaaa tcttgagatc agttgctgtc
     6361 catctgccga agagactttc ttccagaatg ttcgtttttt tccatagcga aaatagatgt
     6421 aaaatacttc tgaagctggc aatatatata cgtcttgggc atgtttggca aaactttcac
     6481 ttatcatttg atccgataat cgttttattt ccattattct cccctccaca aaaaacatac
     6541 gtctttttga tctttttgtt tcaaataaaa tgaatttaca aaaaaatatt ggcaaaaacc
     6601 gaatttatgt tatacttata aaaatttatc ttgggaggca ctccattgga aacaacatta
     6661 aggaaatata tcgaagtagc aagtaaaaaa aagcctgctg acctagtgat caagcatgct
     6721 aagatcgtca acgtctttac gaaagaaatc atggaaaaag atgtagcaat ctgcgaagga
     6781 atgatcgtag gaatcggtga atacgaagga aaaagcgtac ataatgctaa tgggcaatat
     6841 ctagtgccag gattcatcga tggccatgtg catatcgaaa gctcactttt atcaccaagc
     6901 gaattttcaa aagtatcctt gcttcatgga gttactactg tagtgacaga tccccatgaa
     6961 ataggaaaca ttgctgggag tacaggtctt gagtttatga tagaagatgc aagacaaaca
     7021 ctcatgaata tcttttttat gctaccttct tgtgtacctg taaccccttt tgagaccaat
     7081 ggtgcaaatt taaatgcaaa aaaattagcc ccttttttac aaaaacccga agttttaggc
     7141 ttagctgaag tcatgaacta tcaagcagta gcaaataatg aaacagacat cctagaaaag
     7201 atccaattga tgcaccagca taagaaaaaa attgacgggc atgcagctgg tatcggcatg
     7261 gaagagttaa atgtttatcc tgcagcaggt attcggacgg atcatgaagc tacaactgca
     7321 aaagaagcta aagaacgttt ggatcttggt atgtatttaa tggttcgaga agggaccgtt
     7381 gcaaaagatc tggcatcgct actaccggcg atcacacctg aaaattcaag aagatgcttc
     7441 tttgtcaccg atgataaact gatcaatgat ttagtaaaag aaggatcaat cgatcatatc
     7501 gtccgtcaag ctattgcact tggaatcgat ccattacaag ccattcagat ggcaacattg
     7561 aatgcagcag aatgttttga tttgaaaaca ctaggtgcga ttgcccctgg gtatcaagct
     7621 gatttcttct taacagacga tcttaccaag ctgccaattc atgaagtttt ctataaggga
     7681 acattagttg ttcaatctgg gcaactccaa gaccatttat ttgctgatca ttcaaattca
     7741 tatacgtgtt gtctgccaaa actaaacgct caaccattaa atccacattc gttagagctg
     7801 cttctttctt cttcacaagc ccatgtgatc gaaattatcc cgaatagtct agtgactaaa
     7861 gatttggtag aaaaagtgga ccgacaaggc gaacgattca ttccttcaat cgaaaaagac
     7921 caattaaaga ttgcggtgat cgagcgtcac catcaaacgg gaaacattgg attaggcata
     7981 gtgaaaggat ttcagctgaa acaaggagcg atagcgacaa cgattgccca tgactcgcat
     8041 aatctagttg tcgttgggac aaacgacgag gatatgctgt atgctgctaa tcttttgatt
     8101 caaaaaggtg gcgggatgac cgttgtcaac aatcagcagg aaatagcttg tgtttctctt
     8161 cctataggcg gaatcatgac acaggaacct ttttcgatcg tctatgaaca gttgaatgaa
     8221 ttaataacta aagcacatca actaggagca gctaaaacct ttgatccttt tttgacactt
     8281 tctttcttaa cattatctgt catacccgaa ttgaagatca ccgacaaagg attattttca
     8341 ttttctcgct tccaattgat tccaccttgt ggaaaaaccg atgaaataaa tacattttaa
     8401 atcaaataaa tcccagaaaa tacattacat ctgtaatgtt cattttctgg gatttattta
     8461 tggatattaa tgtttttatg aaagcagaaa gttagtttca cagacttttc tttttggtct
     8521 tatcttgaaa ttgagcttct actcgataaa tacgttgacc ttttgaagaa aatttttctt
     8581 catactccgt cataatattg ccttcaaatt gactttgatg taaatctaac catacctgtt
     8641 tgaggatcat gccatattga gaaaaacttg ccagagaata ctcaaacaat ccttgattat
     8701 ctgttttgaa atggatttct ccatttggac gaagaatctg ttcatctaca gcaagaaaag
     8761 tcttatatgt caatcgccgt ttttcatgac gtttttttgg ccatggatcg gaaaaattca
     8821 gatagatttg atctacttcg ctgtcagcga aatactctgt caacgctgat ccatccacat
     8881 gtagaagttt caggttagga agcggttgtt cgatcaactt gtctagtgca atcgaaacaa
     8941 cactgacctg catttctact ccaatataat tgatttcagg atgggctttt gccattccag
     9001 tgatgaactg gcctttcccc ataccaatct caatgtggat tggatgatcg ttttcaaaca
     9061 attcattcca ttttcccttc cacaatgttg gatcactaat tacaaaattc gggtgagccg
     9121 ctaacatttc cgctgctccc ggacgatttc ggacacgcat tatttatctc cattcctttc
     9181 ctctaattaa aaaggtctgg ccaaatctcc gcagagtttg ggccaaacct cagccactga
     9241 aaattcgttt taattgtaaa atttcttcgt tcatccgtcg atgatcttca cgtttgcaat
     9301 atctggtgat ttcttggagc aaattcatgg ccgcatacca atgaaatttt tcaagcattt
     9361 cgtcagtcgg gcgtatgcca taactgatca gccactgatt ccagctagac agtggcacat
     9421 aatttcctaa aatcgttgca atatccaccg cagggtctgc aaacatgatt gaatcccaat
     9481 cgactagata taaataattt ttacatacta accaattacg atggtttaca tctccgtgta
     9541 ctactgataa gttttcctta tctattgacg ggatctgctc acgaagaaaa cgatggacaa
     9601 tttgcagaaa ttgatttttc tggagttctt ccggtaattc tttttcatat ttttgcagca
     9661 tcttctccgg cgtcatgaca cgaccaccga tacgggtcag catggtttta agagaatttg
     9721 aatgatgtaa atggtacaaa acatcaatca cgtcgtttct ttgaccaatc tcattaggag
     9781 tcagcaagcg accttccagc cactcttgtg ctgtcaaagt atcgccattc cccgttcgtt
     9841 ttgtccaaac aagttttggt gtaattcctt cccttgaaag agcagccaac atcggagtcg
     9901 tgtttcgttt aataaagact ttgtcctcat cacgcatgcc tatgtatgtt tttcctgtgt
     9961 cgcctttgat gggacgtaac cgccactctt ggtctaactg aaactccatc atgcattctc
    10021 ccgtcctctt actctttgtt ttgcagtact tattattcta gtcgcctgaa cactacacgt
    10081 caagcacata tacgttcttt ttcatttaat tgccattcaa aaaaccagct tttctcaaaa
    10141 aaaatagaac gaacagcatt cttactgtca acgctctatt tttcattctc atttttaccc
    10201 tacacagctt tgttttcagt ctgacatctt cttcaaacga gttggtacat atagtttaac
    10261 aaagagcata gtaacaatca aataagccgc tagcggaacc catcgctgct gcccatctaa
    10321 tacgactgca gcaatcaaac caaaaataac cgacgcgata attaataccc atgtgagtag
    10381 atactgaatc gcctgcttct tctgattctt atggattgga taaagctgaa cgagaaccat
    10441 atagcggaac tggttcgcta aaggtaacag ttggaaaccg atcaaatata aaaataagaa
    10501 accgataatc agactaaaat aaaagtcgac tactccggca atcaataacc cacctataat
    10561 tactaatcgg aaataaagac cactaaaatc agaacctctt gccaatcttc ttgcaaacaa
    10621 gtaaagataa gtgttttttg attctctttt aatatgagtc aatataggat ccaaatagcg
    10681 tcttcttttg atctttgccg taatttcagg aacatctgtg aataaattga taaattgata
    10741 gatctgatgc aacctatgtt cttctacatg caccattttc tcccaatcca atcctttttc
    10801 ttgttgcttc cacaaaaacc agtaatatag acttgcctgt actaatgcac ctgctattcc
    10861 tgcccatatt agccaataaa atgatagaaa caaaaagatc agagaagaaa aaaaccagcc
    10921 taatttccaa taacgaataa cttgttgatc tagtcgaaac aattctgctc tcttgatttg
    10981 cagatgactg aatttcaaac tccataatat gatcagatag acgaaaaaca tcgaaaagga
    11041 ttgctccgta ctaaccacca ctaatggcat ggccaatccg catgccaata tcaaaaagat
    11101 caacggaaaa atgcaagaat agcgaaaagc tcgttccaaa taggtttgca tttctttttc
    11161 tttaggaagt aaaaaaacaa tatctgctgc ctctgcaaaa gtagccatct tacccacgaa
    11221 taaacagagc atccaaaata cagagattat gagccctcca attgcaaacg gtggctgcag
    11281 ggttttcaac caattagaat aatataatcc cacaccgcca atcaagaaaa gacaaactaa
    11341 aacaaaatga tcgttcaata cataacgcat atatttcatc attttctttt gatgtcttgc
    11401 taaacgtttt tgaaaaaaac tactcatgtg cctgctcctt tgttaaagca atatagattt
    11461 cgtccaaaga agaatccggc agctgaaatt cttttctcaa catctcaaga gaaccgtctg
    11521 ctcgtatctc tccgttatgt aagaccacaa aacgatcaca atatttttct gcagtagcta
    11581 aaatatgcgt agacatcaaa ataccggctc cttctttttt catttctacc atcaattcaa
    11641 gcaatgcatt gatagctagt ggatccaagc ctaaaaatgg ttcgtcgatg atatataaag
    11701 aaggctcgat caaaaaggcg cataagatca tcactttctg tttcattccc tttgaaaaat
    11761 tagctgggaa ccagtcaaga cgattagata accgaaaagt ctccaacaac ttttctgctc
    11821 ttttcattgc tatatccata ggtatatcgt aggccatcgc agtgacttcg atatgttctc
    11881 tcaatgtcaa ttcttcatat aaagaaggag tctctggaat atacccaatt tgtttgcgat
    11941 attcttccgt atttttcttt aaggatagat gattgatttc tatcgttcct tttgaagctt
    12001 ccagcagacc gataatgtgc ttgatggtcg tacttttccc tgctccgttc agtccaatca
    12061 accctactat ctctccaggc tgtacatcga aactgatatc ttttaacaca gggagatgtc
    12121 cgtacccccc tgtgacatga tcgataatta aactcattct ttttcctccg ccatttattt
    12181 ttatttatta taccacgaag aaaatgacgg tattttgttt ttttccttac aatagcttta
    12241 acttatggta aagttaagct gaagtattta ttctttatag aaaggatgtt ttatatggaa
    12301 aactgtattt tttgcaaaat catcaatcaa gaaatcccaa gctataaaat atatgaagac
    12361 gacaaggtct atgctttttt agacatctct caagcaacga aaggacacac attagttgtg
    12421 cctaaacgtc atgtagcaga tatttttgaa tatgatcccg aacttgcagg agaggttttt
    12481 tcgcgtgtgc ctaagattgc tcaagcattg gaaaaagctt ttccagaaat gaaaggtctt
    12541 aatatcttga ataacaaccg tgaactagcc tatcaatctg tttttcattc acatatccat
    12601 ttagtccctc gctacagcaa agaggatgat ttttctattc actttgtcaa tcatcaagat
    12661 agttatggat cggaagaact aaaagctatt caagaaacaa tcgtaaagca ggtgtcctgc
    12721 gatgattaaa aactttttca aaggactgtt atttggagct gctgctggca ctgtcggagg
    12781 tctgttattg gcgcccagga gcgggaatga aacaagaaag aaactgatcg aagaattgga
    12841 agaagcaaca actctgacaa atgatttaaa taacagcctg aatcatttta aaagcgcatt
    12901 gatcgaaaca aaagaaacag ctgaatctgt gattcctgct ttccaagaag caatcaaaaa
    12961 agatatcgaa gattttaaat tccaagccga accacgtatc acacaaatca atgaacaagt
    13021 agaaaaacta aaagctgatc tgccagagta tcctgaacaa atataataat gattattttc
    13081 tcaacagatt taactatata agtgagaaaa gtttgcgtat ttcctaggaa tgtgatgaaa
    13141 ttttgaattt tgtttgaaac taccacgaca acgtttttaa aggaaatggg atatggtata
    13201 gttaatatat aaaaataata actaattaca ggagtgcaaa ttttaatgaa aaaaaaatca
    13261 attattttgg cagctactag tgcgttggct gttttaactc tagcagcttg ttcaggcgat
    13321 actaataaag atatcgcaac gatgaaaggc ggaacgatca ctgtatctga tttctatgat
    13381 gaagcaaaac ttgaatcatc gaaccaatcc ttagtacaac ggatgatcat ttataaagta
    13441 ttcaataaca aatatggcga taaagtaacc gataagcaag tggatgcaga atacgacaaa
    13501 caagcgaaat cattaggtga tacttttgaa tcacaattag aagctgctgg atatacgaaa
    13561 gatacttata aagaatatat tcgtaacaat ttagcttttg aagctggctt gaaagctcac
    13621 gtagatatta cagatgacga tttgaaaact gcttggaaat ctttccatcc agaagtagaa
    13681 gcacaaatca tcaaattatc aagtgaagat gaagcaaaag atgtgaaaaa atcagcggat
    13741 gatggagatg atttctctaa attagccaaa gacaaatcaa ctgatacaga gacaaaagaa
    13801 gatggcggta aagttaaatt cgactcaacg acaacaacta ttcctgcaga agtaaaagaa
    13861 gcagctttca aattgaaaga cggtgagatc tccgatgtta ttactacaac aaatcctact
    13921 tcttatgcga cagaatacta cgttgtcaaa atggtgaaaa accaaaataa aggcaatgac
    13981 atggataaat acaaagatca actaaaagat atcgctactg aaactaaact aagtgacaat
    14041 gcattcacaa ctaaagtaat cggtgaagaa ttaaaagatg caaatgtaaa aatcaaagat
    14101 gatgcctttg aaaatgtgtt gagtgcattc acaacaactt catcaagtac gaaagactca
    14161 agtgaaacta cagcttcaac aaagagttct gacacaaaat caactgattc aacaaaagaa
    14221 tcttcaactg aagaaacaac tgattcatca aaataaa
//
LOCUS       DAYYME010000067        14243 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000067 DAYYME010000000
VERSION     DAYYME010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.068"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACHBFW_001703"
     CDS             complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACHBFW_001703"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFM9318008.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACHBFW_001704"
     CDS             complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACHBFW_001704"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM9318009.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8118..9086
                     /locus_tag="ACHBFW_001705"
     CDS             8118..9086
                     /locus_tag="ACHBFW_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9318010.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9104..10009
                     /locus_tag="ACHBFW_001706"
     CDS             9104..10009
                     /locus_tag="ACHBFW_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9318011.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10006..10812
                     /locus_tag="ACHBFW_001707"
     CDS             10006..10812
                     /locus_tag="ACHBFW_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9318012.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10969..11937
                     /locus_tag="ACHBFW_001708"
     CDS             10969..11937
                     /locus_tag="ACHBFW_001708"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFM9318013.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12254..12778)
                     /locus_tag="ACHBFW_001709"
     CDS             complement(12254..12778)
                     /locus_tag="ACHBFW_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFM9318014.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13065..>14243
                     /gene="scm"
                     /locus_tag="ACHBFW_001710"
     CDS             13065..>14243
                     /gene="scm"
                     /locus_tag="ACHBFW_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM9318015.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     S"
BASE COUNT         4037 a         3079 c         2810 g         4317 t
ORIGIN      
        1 gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca
       61 ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg
      121 ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga
      181 tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga
      241 tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat
      301 ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc
      361 ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac
      421 ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa
      481 ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta
      541 cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg
      601 gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt
      661 tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta
      721 cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg
      781 tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac
      841 cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag
      901 taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt
      961 cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac
     1021 atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga
     1081 tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta
     1141 cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc
     1201 cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga
     1261 ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca
     1321 aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg
     1381 atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg
     1441 ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag
     1501 aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga
     1561 tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga
     1621 tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga
     1681 tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg
     1741 tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt
     1801 tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata
     1861 ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt
     1921 ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag
     1981 gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc
     2041 attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat
     2101 gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt
     2161 cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc atatcttggg
     2221 aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca
     2281 acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc
     2341 catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt
     2401 gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca
     2461 gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac
     2521 gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac
     2581 gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca
     2641 tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta
     2701 gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg
     2761 ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta
     2821 ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta
     2881 acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac
     2941 ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag
     3001 ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa
     3061 tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt
     3121 tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg
     3181 cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt
     3241 ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag
     3301 caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg
     3361 aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca
     3421 tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc
     3481 ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa
     3541 aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct
     3601 caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta
     3661 tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat
     3721 ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta
     3781 aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt
     3841 cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat
     3901 caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt
     3961 cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga
     4021 atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt
     4081 tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg
     4141 caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag
     4201 ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca
     4261 atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc
     4321 catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac
     4381 cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg
     4441 ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga
     4501 tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag
     4561 gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac
     4621 gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta
     4681 acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt
     4741 gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt
     4801 tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg
     4861 gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac
     4921 ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt
     4981 cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag
     5041 tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc
     5101 cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca
     5161 tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg
     5221 gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat
     5281 ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac
     5341 cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag
     5401 taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac
     5461 ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg
     5521 caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt
     5581 tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg
     5641 ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt
     5701 ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga
     5761 atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag
     5821 gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac
     5881 cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca
     5941 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
     6001 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
     6061 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
     6121 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
     6181 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
     6241 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
     6301 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
     6361 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
     6421 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
     6481 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
     6541 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
     6601 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
     6661 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
     6721 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
     6781 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
     6841 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
     6901 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
     6961 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
     7021 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
     7081 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
     7141 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
     7201 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
     7261 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
     7321 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
     7381 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
     7441 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
     7501 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
     7561 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
     7621 agtttttgca acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt
     7681 tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga
     7741 caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga
     7801 ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac
     7861 tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt
     7921 tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct
     7981 aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta
     8041 ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa
     8101 ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac
     8161 tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa
     8221 caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca
     8281 aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa
     8341 atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc
     8401 ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca
     8461 ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca
     8521 atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa
     8581 tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg
     8641 gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg
     8701 tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc
     8761 tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa
     8821 gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc
     8881 aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggtaaacaaa
     8941 cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa
     9001 cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc
     9061 cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc
     9121 tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata
     9181 cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg
     9241 tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct
     9301 aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa
     9361 aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg
     9421 tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga
     9481 gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat
     9541 cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac
     9601 tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat
     9661 cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg cgcaaaacaa
     9721 cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat
     9781 catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt
     9841 aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag
     9901 tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca
     9961 gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta
    10021 acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg
    10081 aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg
    10141 gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa
    10201 attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc
    10261 agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat
    10321 cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa
    10381 gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta
    10441 tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc
    10501 acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct
    10561 acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct
    10621 tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg
    10681 catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc
    10741 caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt
    10801 ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt
    10861 ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt
    10921 gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa
    10981 gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca
    11041 cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca
    11101 ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat
    11161 gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg
    11221 ttgacttctg aatctacgat gaaagatgtc aaactggcag cagctcaagg agattctgtt
    11281 tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt
    11341 ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt
    11401 gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct
    11461 gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa
    11521 gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc
    11581 atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa
    11641 gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc
    11701 agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc
    11761 tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact
    11821 tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa
    11881 cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta
    11941 tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca
    12001 aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta
    12061 aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag
    12121 tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata
    12181 gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt
    12241 tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag
    12301 agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg
    12361 tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa
    12421 tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga
    12481 tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg
    12541 gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa
    12601 tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg
    12661 gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac
    12721 gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt
    12781 attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata cctacttata
    12841 tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga
    12901 ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt
    12961 cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa
    13021 aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa
    13081 gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag
    13141 cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg
    13201 gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg
    13261 cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag
    13321 agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg
    13381 ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta
    13441 acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact
    13501 taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg
    13561 aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat
    13621 atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag
    13681 ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc
    13741 aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat
    13801 tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt
    13861 cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc
    13921 cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg
    13981 gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg
    14041 aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat
    14101 ctaccacaag tgatagctca gattcatcaa cttcaagcga cagtacagat actacctcta
    14161 ccacaagtga tagcacggat acatcagctt caagcgacag tacagatact acctctacca
    14221 caagtgatag cacggacaca tca
//
LOCUS       DAYYME010000068        14189 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000068 DAYYME010000000
VERSION     DAYYME010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.069"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACHBFW_001711"
     CDS             complement(<1..1835)
                     /locus_tag="ACHBFW_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM9318016.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACHBFW_001712"
     CDS             complement(1832..2536)
                     /locus_tag="ACHBFW_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM9318017.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACHBFW_001713"
     CDS             complement(2548..4857)
                     /locus_tag="ACHBFW_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM9318018.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACHBFW_001714"
     CDS             complement(5052..5516)
                     /locus_tag="ACHBFW_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318019.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACHBFW_001715"
     CDS             complement(5516..6142)
                     /locus_tag="ACHBFW_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM9318020.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACHBFW_001716"
     CDS             complement(6149..6514)
                     /locus_tag="ACHBFW_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM9318021.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACHBFW_001717"
     CDS             complement(6504..6842)
                     /locus_tag="ACHBFW_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318022.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACHBFW_001718"
     CDS             complement(6832..7158)
                     /locus_tag="ACHBFW_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM9318023.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7139..7417)
                     /locus_tag="ACHBFW_001719"
     CDS             complement(7139..7417)
                     /locus_tag="ACHBFW_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM9318024.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7419..8783)
                     /locus_tag="ACHBFW_001720"
     CDS             complement(7419..8783)
                     /locus_tag="ACHBFW_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM9318025.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACHBFW_001721"
     CDS             complement(8796..9365)
                     /locus_tag="ACHBFW_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFM9318026.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACHBFW_001722"
     CDS             complement(9337..10524)
                     /locus_tag="ACHBFW_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM9318027.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACHBFW_001723"
     CDS             complement(10588..12315)
                     /locus_tag="ACHBFW_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM9318028.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACHBFW_001724"
     CDS             complement(12312..12764)
                     /locus_tag="ACHBFW_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM9318029.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACHBFW_001725"
     CDS             complement(12876..13256)
                     /locus_tag="ACHBFW_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM9318030.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACHBFW_001726"
     CDS             complement(13253..13639)
                     /locus_tag="ACHBFW_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFM9318031.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACHBFW_001727"
     CDS             complement(13641..13919)
                     /locus_tag="ACHBFW_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318032.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13982..>14189
                     /locus_tag="ACHBFW_001728"
     CDS             13982..>14189
                     /locus_tag="ACHBFW_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318033.1"
                     /translation="MLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGVSSYSA
                     FDNDRIFGVLVQCINDECNKFYSLQ"
BASE COUNT         3924 a         3056 c         2233 g         4976 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaat
//
LOCUS       DAYYME010000069        13838 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000069 DAYYME010000000
VERSION     DAYYME010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.070"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            151..798
                     /locus_tag="ACHBFW_001729"
     CDS             151..798
                     /locus_tag="ACHBFW_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM9318034.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            882..1826
                     /locus_tag="ACHBFW_001730"
     CDS             882..1826
                     /locus_tag="ACHBFW_001730"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFM9318035.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1961..3412
                     /locus_tag="ACHBFW_001731"
     CDS             1961..3412
                     /locus_tag="ACHBFW_001731"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFM9318036.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3433..4269
                     /locus_tag="ACHBFW_001732"
     CDS             3433..4269
                     /locus_tag="ACHBFW_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM9318037.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4269..4928
                     /locus_tag="ACHBFW_001733"
     CDS             4269..4928
                     /locus_tag="ACHBFW_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9318038.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            5054..5743
                     /locus_tag="ACHBFW_001734"
     CDS             5054..5743
                     /locus_tag="ACHBFW_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318039.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5749..6705
                     /locus_tag="ACHBFW_001735"
     CDS             5749..6705
                     /locus_tag="ACHBFW_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFM9318040.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6749..7261
                     /locus_tag="ACHBFW_001736"
     CDS             6749..7261
                     /locus_tag="ACHBFW_001736"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9318041.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7517..8065)
                     /locus_tag="ACHBFW_001737"
     CDS             complement(7517..8065)
                     /locus_tag="ACHBFW_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM9318042.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8154..9335)
                     /locus_tag="ACHBFW_001738"
     CDS             complement(8154..9335)
                     /locus_tag="ACHBFW_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFM9318043.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9571..11079)
                     /locus_tag="ACHBFW_001739"
     CDS             complement(9571..11079)
                     /locus_tag="ACHBFW_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFM9318044.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11238..11429
                     /locus_tag="ACHBFW_001740"
     CDS             11238..11429
                     /locus_tag="ACHBFW_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318045.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11632..12549
                     /locus_tag="ACHBFW_001741"
     CDS             11632..12549
                     /locus_tag="ACHBFW_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFM9318046.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12542..13615
                     /locus_tag="ACHBFW_001742"
     CDS             12542..13615
                     /locus_tag="ACHBFW_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318047.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
BASE COUNT         4615 a         2522 c         2728 g         3973 t
ORIGIN      
        1 aacttctagg attgtctcta aagcttagtt tctttttctt tatattttca taataaaagt
       61 ttggttcttt aatttttaaa catgacagaa aattttttct tcgttatact tgtaatagaa
      121 tactatttta taacaaaggg gatcgaatca atgaaaaaaa ctgttattta tactagttta
      181 tttgcagctt tgtttttagc tgcttgtcaa tcaaatacca atcaaggcac atctgggaat
      241 acgtcttcca gtagcgaaca gcgctcttca caggtatcgg actcctcaac aaaagcaagc
      301 acatcttcgt ctagccagac aaaaagttct agtgaagcaa gtactcagtc agatatcaaa
      361 gtgtctgcag ctcgtgcgat cgaattgttc caagataaat acccagatac tgcaattact
      421 agtttggaat tagatagtga ttggggatct tacttctaca agatagaagg tgtcgatgat
      481 caaaaagaat acgaagcaag aattgatgct caaaatgaaa aaatggagac agagaatcca
      541 gaaactctag atcgagatga acaaaatggt caaaagaaaa aagaagatgg tctggatgtt
      601 gataaattga tcacgattga ggaagcagca aaaatcgccg tcgacaaagt aggaagcggt
      661 acggcaacag attgggattt ggataaagat ttaggtacta cttattggga agtcaaagta
      721 aaagacggca atcaaagtac atcagtcaag atcaatagcc aaacaggcga agtactgtca
      781 actgaacaag atgattaaga atttcttcgg aaaacaagag ttgatttttc ttttagtcat
      841 gatacactag acaaaataac ttgtttgaag gagcgattta cttgagttta gaaacgacct
      901 ttgccaatca tatctttgcc aatcctttga tgaatgcatc tggtgtccac tgcatgacta
      961 cccaggaatt agatgaactg gctcattcag aagcaggagc atttatcact aagagctgta
     1021 cgatcaatga acgaaaagga aatcccgaac cacgctattt tgatgttcct ttagggagta
     1081 tcaattctat gggacttcca aacttaggtt tttcttatta tctagagtat gctcttgcgt
     1141 atgaaaaagt gcaggaaaac caaaatcaac cgttattttt ttccattgcc gggatgagtg
     1201 tccaggaaaa cttggaaatg cttgagaaaa tagaaaaaag tgggtttaat gggatcactg
     1261 aattaaattt atcttgtccc aatgtgcctg gaaaaccaca actagcttac gattttgagg
     1321 ccacttatga aacactgaaa gaagtattca gcatcttttc taagccgcta ggaatcaaat
     1381 tgcctcctta ttttgatttt gctcattttg atcagatggc tgatatcttg aatcagtttc
     1441 cattgaccta tgtcaatgct atcaacagtg tgggcaatgg tttgtatatc gatacagaac
     1501 aagaagcagt agtcatcaaa ccaaaagaag ggttcggcgg cattggcgga gagtatatca
     1561 agccgactgc tttggcgaat gttcgcgctt tttatacacg tttgaaacca gaaatccaaa
     1621 tcattggtac aggtgggatt cggacaggac aagatgcatt tgaacatctg ctatgtggcg
     1681 caagcatgtt acagattggt accgagcttc ataaagaagg gccggaaatt ttttctagaa
     1741 tcataaaaga attaacgcag attatgtcag aaaaaggata tacgtcaatt gacgaattta
     1801 aaggcaaatt acgcaccatt tcatagcaaa gagacgaagg tcatgattga tcttcgtctt
     1861 tttttataaa aaacactcgt ctgttcattc ctgtgcaagc gtactcattg tatgttagac
     1921 gaacttgtgg gaaaataaca gaggcaaaaa ggaggataat atgaatgatg caacagaatt
     1981 agcaaaagag cttaaagaaa aagaatttag ttttcaagaa ctagttacgg atatacagaa
     2041 aaaaataaag aagcaaaatc atgaattgaa tgcgtttgtc gcgcttgaac cattcgagag
     2101 taattcccac gagctattga ttccccagtc agaaaaaggg ccattggctg gtatcccatt
     2161 tcctttaaaa atgctcggtc aagaaaaaaa gggatggcta gctacttcag gatctcgttt
     2221 atttgaaaca caccgagctt ctcatacatc taattatgtg cggcaagcag aagcgatagg
     2281 tctagttcct tttggacaaa ccaatgcgcc agagtttggt ttcaaaaata ttacagaccc
     2341 agtcatctat ggcccagcaa gaaacccttg gaaccttgaa tgtacaccgg gcggatcaag
     2401 tggaggagct gctgctgcag tttcatcagg aatcgttcct ttagctggtg ctagtgatgg
     2461 cggtggttct attagaatac ccgcttcgtt ttgcggattg attgggctga aaccttcgcg
     2521 agggacgatg ccagttggtc catatgcttg gagaggatgg caaggagctg ctattgattt
     2581 cggcttgaca gtttctatga gagacacgga ggctttattt tatgggatga gaagtatcca
     2641 ttccggtgcg ccatatcaag tatcgccagt tgagtggcaa acacatccaa gaaaaaaacg
     2701 gttgaagata gctttttgtg ttgattcacc gatccactca cagattagtg aagaggcagt
     2761 acttgctgta aaaaaagctg tagatttcct tggaaaagaa gggcatgatt tgacagagat
     2821 tccttaccca ctagatggtc gtaaactgat cacgtcttat tatcagatga atggcgcaga
     2881 aacagcagca atgatccgct cgattgaaac aggcatccag cgagctgtca gaaaagaaga
     2941 acttgaaccc atttcttgga ctttgttcca atatggtcag aagctctctg ccgctactta
     3001 catcgattct ctgcatgtat gggatgaagc agcaattgtc atggaaaacc tacatcaaga
     3061 atacgatcta ttcctttcac caacgactgc atttaccgcc cctaaaatca gcgaagattt
     3121 gcaaagtgat gcgataagag cacagatgga acacatcagt gattgttctg aagtagaagg
     3181 tttagacgtt gtttcttcca tgtttgagga gagcttgaag atcacacctt atacacagtt
     3241 ggctaatctg actggacaac cagcaatcag cttgcctact catttgacaa aagaaaacat
     3301 gcctctagga atccaattta tggctgccag aggaagagaa gatttgctat ttcaaatcgg
     3361 ctatctattt gaagcaaatc atcagttcca tttaccaaat ccatggaaat agcagaaaca
     3421 aggagaagaa gaatgaagaa aaaaagattg tttgctctaa taactagtgc cttttttatt
     3481 ttaagcgctt gcggtacagc tgccgctaat caggaagata acagctggag taatgtagaa
     3541 aaaaagaaag aattagtagt agcgacttct ggcacactgt ttcccgcatc ttattataat
     3601 gatcaaaatg aattagttgg ttatgatgta gatttggcaa aagaggtggc taaacggtta
     3661 aatgtcaaaa tctcctttaa agaatacaac gtggatggac aaatcacttc tgtttcaaaa
     3721 agagaatcag attttgctgc gaacgatttt ggtatcacaa aagaaagaga aaaaaaattc
     3781 tccctttcag agactttcaa atattcattt gacagtatga tcgtacgaaa aaaagatgat
     3841 tcaggaatcc actcacttga agatcttaaa gggaaaaaag cagctggcga acccaatact
     3901 agctatatgc gtttagcgga aaaacacgga gctaaattag tcacatatga caatgccacg
     3961 aatgatcagt atttaagcga tgtagcaaat ggtcgaacag atgttattct aaatgattat
     4021 tatctgcaga aaatggccgt atctgcttta ccagatatcc ctgtaaaaat actggagaac
     4081 gtttatttca atgaaaacga aagtggtttt ttattgaaag aaggaaatga tgctctgaaa
     4141 aagaaaatcg atggcgtatt aaaagagatg aaagaagatg ggacgacaaa gaaaatatct
     4201 gaaaaatatt ttcaaactga tgtttcagtc aaaccgaaag aaacgatggc tgagtcggtg
     4261 agtgagtgat gtactttcct ctaattgatt tttccctcat gtggacttca cttcctttcg
     4321 tactgcaagg cttggtatac acattaggga ttggcttcgt gtcgtttctt ttaggaaatc
     4381 tggtagggtt gcttttgaca gttttaggtc ttttggattg gctgcctctg aatgtattta
     4441 ttcgttttta tttatcgttc tttagaggca ttcccgcact agtcctgctg tttctgttat
     4501 attttgggtt accttatcaa ttaagtgcct taacggcttc agtcatctgc tttacgatta
     4561 ccagcagtgc atttattggt gaaatctatc gaggatcact agcaggcgtt tcttcaggtc
     4621 agtgggacgc tggtttagct tctggttttt ctttctttgg ggtattgaaa tatatcgttt
     4681 tgccgcaagc ttttcgaatc tcagtaccgg cactcagtaa cgtagctatg gatctagtga
     4741 aaggtacttc gttagcagca acaatcacag tgccagaaat atttcaaaaa gcaaagatca
     4801 taggcggtcg gacattcgat tatttttctc tctatatttt agttgcactg atttattggg
     4861 tcatttgtat cgggatcgaa aaagtgcaac agtatttaga aaaacgttct atgcatcagt
     4921 caatctgaaa aatagaaatg gtgctagcgt ggctggcacc atttctattt tttgtttctt
     4981 gtatattctg cctagtattt taaaaccatt tcatgtaaaa tgtaaatcga aaatatgttc
     5041 ggaggtagaa aacatgaaga ccccgctaac catcgaaata gaatcctcgt tataccaata
     5101 ttgcatcgag caaggcggct tggttgtaga agaagtcacg atgcctgatg accaaggaat
     5161 tgtagatacg cttagctgct tgacaaaatt tgatggaagc cgagaatggc gatgctatga
     5221 gctgaaagtg acaaaagcag attttcgctc tacagccaaa ctgtcatttg ttggacatta
     5281 taattatttt gttttaccaa gtaaactata tgaagaagta aaaactgaaa tccctacagg
     5341 aataggtgtt ttagtgtatc gtccgtacac ggttgaagag gagatgcctg cctctggtac
     5401 atttctgatt gcgaaaaaag cagcaaagaa agagcttgct gtaccggaag aagctttgac
     5461 aaaccgcttc atggcttctt tattcaggga agtaagaaaa gcaaagcaaa tggagcgagg
     5521 cgtaaaatat tttccaacag atcaattgta caaagaattg aaaagacgtt cagaaaatca
     5581 agatccattt tctaacgaac gatattatga acggttttta gaggaaatag ctagtcaggc
     5641 aatcaatgag ttgcaagaag aagttatcgc gcttcagcat gattatgaat atttgaggca
     5701 acaacagcag atccgtcggt tgcctacaga gccattggag tgaaaaatat gtactatcct
     5761 tatttaagag gaaaacaatt tgacctatta gcattaaaag aagcactgtc gcgaggattg
     5821 ctctccaata aaatccaacc agtgatcgaa ccagtacgtg attccgctac gcttaaaaat
     5881 gtgatcgaat tatttcaaaa aaagcaccat cgaatcgctg tgatccaaaa tccccaagta
     5941 ggacaattca aattattcga tcagcatgtg cacagttgga cagtcaaaga aaatagctct
     6001 gtcgtccctg cacagatctt aacgccagaa aatctcagtg aagtactgga ttcacctcca
     6061 gcgtttcttg ttttcgatgg acagcactac ccaaaagata cagaggtgtg gaaacagttg
     6121 gcaggagttg attcgaaatt tttgatacca gatactagtc gctttcgcat ctggctgccc
     6181 gaaaacaaaa ttgtgatccg tgactctttt caaacgagaa agcatgttga aagttatgca
     6241 gataaaacag atgacttttt tagtgatgac tatctttttt tccacgcaga cggatatatt
     6301 ggattttctg atttcacgat cgaaggtagc cgttatttcg ataaagggtt ccctagccga
     6361 gcgcttgcta tccatctaac gtatattgat gcttacggca atatacgcgt gaaacatttt
     6421 gtctctgaca gtaacgactc tgctaaagac caagcattga agtttcttga agcaggagat
     6481 aaaatgaaaa aatggatcat gcgtcaccat catcaactct tgattacttc aggaatgatt
     6541 gaattattgg ctctttacca gcaacaaaaa ttccctggac tgggagtgtt gaaaaagtgg
     6601 tcacttatgc atcatttgga acttattagt cacctgctag atcatccaaa agattggctt
     6661 aaatattatt caaaagttca ggaactatat gaagtgatcc aatgattaga taatgtaaat
     6721 gaaaaaggaa tagaaaagag gaaactggat gtatattcgg caaacaagga aagaagaagt
     6781 agaagaagtg gtagaaatca ttaatgccgc agcggatttc attgcttcgc aaggctctcc
     6841 tcaatggcaa aacgggcaga aaccaacaaa agaaacgatt ttgaacgata ttgaaagaaa
     6901 agaaagctat gtattaatag tggaagatca aattgctgga acagctgcat tagtctcaag
     6961 cgtggatccc gtttacacaa aaatagatgg tagttggaaa gaatcaactg gtccttattt
     7021 atcgattcat cgtgtcgctt tagcagaaag tactcgagga aaaaaactag caaaaccgtt
     7081 cttagaacat ttgttgacga ttgcagccag caaaaatatc caagatatcc gtatcgatac
     7141 ttatccagct aatgaaccga tggaaaagac catctattct gctggattca cttatcaagg
     7201 gatgattgaa tttccttttg aacacggcga aagaaaagca tatcaaataa tcatcaattg
     7261 actataaaat catctgagca aagataaaga cattaacact actgaaaaaa acaatcatat
     7321 gatagccaag aggttgtaac agaagtggcc agcttcaaaa aacaagaaag aatttccgaa
     7381 aatgcttctc aaatttttgt ggaattttag cgtattttac gaaatagctg ctttctgaaa
     7441 ctcccttcat cgagaggtcg tgacatagct tttgtcacga cctctcggct tatcctacga
     7501 gtattattcg atacatttat atcaataatc ctttttttat tttatctgca gcttcttgga
     7561 acgctccatc gtttgacacg actaaaagtg aggggatagc tttcaaacgt ttcaactgtg
     7621 ctaaatcttc tacataaacc gccagtctgg ctgaaatctg ttcagatgaa tctccacgtt
     7681 ttttcaggcg cgattgcaat gtttctttag agatcgtaat aaagatagga cggactaccg
     7741 tcccgaaata ctcatataaa tgccaaaaac catttgctgt caaaacggcg taacaatttc
     7801 ctactgtcag tttcttttta atttctgctt tactggaacc atatttatgt tgagcatatt
     7861 ggtcccactc tgcaaactca tttcccttga tcatctgatt gaatgtattg tcagaaacaa
     7921 aataataatc ctcttgatct ttttccccag gtcttggtga tcgattcgtg taagtaatga
     7981 tcttttgttc cggctgaaaa atagcttctg cgagtgaagt ttttccagaa ccagaaggac
     8041 cgataaatac atagcaataa ttcattgtca tttctccttt gatgattgaa caagtgaaaa
     8101 agctttggaa aatatttcca aagcttttaa aagtaacttt gcttttttgt atattatacc
     8161 agcaagccag tcacaatagc acttaaaaag ctgactaaag tcgctgttag caaaattttc
     8221 aatccgtgtt ttgcgacaag atttcctttt tcttcattta atcccttcat cgcaccagaa
     8281 atgatcccga tagaagagaa gttggcaaat gaaacgagga agacagatat aattgctgtt
     8341 gtacgctcag taaaatggaa attaccattt gctaattctg tcatagcaac aaattcattt
     8401 gtaaccagtt tagtcgccat gatactaccg gcatccactg cttctttcca tgggattccg
     8461 ctgatgaagg caagaggggc aaagatgtaa cctaggatct cttggaaagt gatgccgaat
     8521 atcccgtgga agatagcatt gatcatggca atcaaagcaa caaaaccgat aagcatagca
     8581 gcaaccgtga tcgccacatg gaaaccatca agaatgtatt ctcccaacat ttggaaaaat
     8641 gtctgttctt tgttttcttc aatgatcagc tcatcttctt tttcttctaa tacataggga
     8701 tttacaattg aagccagaat ataaccgcca aataagttca aaacaagggc agtgatcaca
     8761 tatttagaat cgatcagtgc catataagat ccgacgatcg acatagaaac tgtagacata
     8821 gccgatacac tcatggtgaa caaacgtttc tcagatagga agggtaattc cttttttatg
     8881 gaaataaaaa cttccgattg tcctaaaata gcagaagcta ctccattata agattccaat
     8941 ttgcccatcc cgttgatttt acttaatcct agaccaacag ctttcataaa taatggaaga
     9001 atcttgatat agcgtagaat accgataata gcagaaataa atacaattgg catcaatacg
     9061 gaaagaaaga aaggtgcact tcctttattt gctacaccgg caaaaacaaa gtcaacacct
     9121 tgcccagcaa aagcaagaag atgatcaaac atttttgcca atacagaaac aaccgctgta
     9181 ccaaaagttg tacgtaataa tacgaaacca agaacaagct gtaaaacgaa catcacaatt
     9241 agtggtttca atttgattgc ttttttgtta ctgctgatca aaaaagcaag ccctaatgtg
     9301 acaaataaac caataatacc taataaataa ctcaaataat cactcctatt ccaaattccg
     9361 ttttatttta cctgttttgc aaataaaata ctaatcttta gtgaaatttt cattactttt
     9421 ttagagtgaa acgcattcat tttccattag aaaataaagc gaacaaaata aaaggtttaa
     9481 aaagtgtaac gtcttagtaa agtctataaa aaaacgaaac tatcgattaa gctaatagtt
     9541 tcgttttcaa catttataag taaacttttt ctattggtaa tatagatttt cggaagggta
     9601 gacaggtgcg caggtaatat cgattcctag ttttttcaat gtttgttcat catttttatt
     9661 gaccatcaca gtagagtggg cttgtgctcc ctcaagttca gctagtttat cataagcaac
     9721 ttgagcgatt ggattcgtta ctgcactgat tgataaagcg atcaagactt cattgatgct
     9781 tagtgcatcc aaagaggaat gaagtgtctt tgatttcaat ccttgtattg tttcaagaac
     9841 taatggtgac aataaaggaa tgtcatctgc gatatgggct aaatgtttta ctgcatttaa
     9901 gataactgcc gctgacgcat ccatcaatga acttgtacgc ccggtaatga tgacttcatc
     9961 tggtaattcg attgcaatca cagcagaagg atcttttgta ggactttgcg cttgaatctt
    10021 atctgcatag tcatgggcag ggactaccac gtttcggtct tcaggacgta aaccgacttc
    10081 ttccataatc agtttgattc gattaactgc ttcttcgtcg atcaatcctt ttttgaagtc
    10141 gcattctgtt tggaaataac gacggatgat ttcttgttta gaggcttctt ggacaacggt
    10201 atcatccacg atcccaaatc ctacacgatt cacacccatg tcagtaggag attgataaac
    10261 agattcttta ccagtaattt tttcaataat acgtttgatt acaggaaatg tctcaacatc
    10321 ccgattatag ttcacagcta ctttattgta ggcatcaaag tggaaggaat cgatcatatt
    10381 gacatctttt agatcgactg tggctgcttc ataagcaatg ttcagcggat gtttcaacgg
    10441 aacattccat actgggaaag tttcgaactt ggagtaacca gctgcatgtc ctttttggct
    10501 ttcatgatac agttggttca aggaagtagc gagctttcca cttcctgggc caggagctgt
    10561 cactacaacg attggtttag ttgtttcaat atactcattt tttccaaaac catcttctga
    10621 gacgattttt tctacgttga ctggatagtc ttcgatttct gagtgtttat agaccttgat
    10681 gtttcgtctt tctagtttat tgataaagac tttcgtggca ggctgtccac tatagcgagt
    10741 gataacgaca ctgttgatta ataagccata accacgtaat tcatcgatta accgcaagat
    10801 atccatatca taagtaatcc cataatcacc acggatctta ttacgttcaa tatctccagc
    10861 atagacgcaa atcagtattt cagcttgatc tttcaatttt tgcagcaatt tgattttcgc
    10921 atcttcttca aatcctggaa gtacacgttt cgcatgcttg tcgtcaacta gcttcccacc
    10981 aaattctaaa tataatttgt catagtggtt cactcgttcc aaaatatacg ctgactgctc
    11041 ttcaatatat ttttctgaat caaaaccaat ttttttcact aggttccctc cttaagtact
    11101 aaagcatttt ttcatttttt tgaggattcg tcaactggaa gagcgaaatt cctcaaaaaa
    11161 acaaagaaaa tggtttaaac caacttgtct agatagttat tcattggtat gatagttttt
    11221 gataaggaag gtgctacatg aaaaaaagac agaaaaagaa aaacgcatat aaacactata
    11281 tccgttcgat ttttaccggt tatgaaagaa tgcttgaaga tcccgaattg gaacaattga
    11341 cgtttactta tctaaacgaa gaaacgcagc taactcgtga tgagcatcag cgcattcatt
    11401 tcacaacaag agatctgcct agtaaataaa aaataagata aaggtcatcg gattcgattc
    11461 tgtcctatca gatggaaaga ctagttgatc gaaaaaaagg ctgtaccaaa ttttggtgca
    11521 gcctgtttta tggagcattg cccgctcttt tttataacaa ttttttacag aaaatggtac
    11581 tattgaagta ttaataagtg aaaaacagat gagtaagagg aggcaagtag tatgaaagag
    11641 ccagatacaa atgcaaatta tgagccagac gaactagagg agacgaatgc ggatcaggat
    11701 acaggacaag aatcgtcttc tttttacgat atcaaagaaa aagcaagcat gaatctttcc
    11761 caattattag aagacttaca ggcttttgaa aaagctgtcc gcgaagaaga tatccctgag
    11821 atctaccgtt tatacaatgg ccgattgaat gaagaattaa aaagaagctc aaatgaaaat
    11881 cacgagatcg atcaattgct gatgcgtaag atccatgatc gttttttaca ggaatttcct
    11941 tttatggaac aagtagaaaa catctcgcca acgatgagct attacaaaat cggcacttat
    12001 taccatgatc ggcccacggt cggtattgat gcaagtctgc cagaaatttt tgttttgcct
    12061 aagatcgatg aagagtggga gaaatattcc caaccggagt caacggacta tgacaaaaaa
    12121 atcaatgagc tagacgcaaa ggtgatcact gctcagacag aaattgaacg tttagatgaa
    12181 caaataaaag aaatcaatcg acaaatgact gatttgaacg acaataaagg attcttgaat
    12241 cgtaaaaaaa tcgaggaaga aattcaagaa ttagagaaga agaagcaagt actatctaat
    12301 gaaaaattag gctggcttcc ttatattgag acgcctgaaa caatccagca gcaaaaagaa
    12361 gcattgaagc aagaagcaag agcagaccag cttcgtgcag ccatcgtaga aaaagaacaa
    12421 aggcaaatca gacgctattt cggtagcaaa gagggattcg gtcaagcaat ccatgaattc
    12481 ttgatgaatt atcttggcaa tgagaatcct aataatcaat ctaatgaagg aggttccgag
    12541 tatgaatgaa cacgatgaag cagaatggac aaaaaatgaa gaacaaacag aagatcatga
    12601 agaaacgaac acttcattgg aagaacaaac aagtaatcct ctggatgaca cagaagaaat
    12661 ccctatgcta gaggaaacaa aaatacacga aaaagaaaaa gtaactccga cgaatacgaa
    12721 tactgcagaa aacacccatg cttacgaaat aactgaaaat cccgggtatt tagaaggaaa
    12781 agagcaagat catcaagata tcgtcagttt gattgaaaca gcggaagcag aattagcgga
    12841 tttacgtatg agaaaagatt tgatcgaatc agattttcca caattattag aatcatatga
    12901 tcgttttata ttgaatgaag aaccaatctc cacgatcgga aagacaagcc tgatcgaata
    12961 cttcacgtat gaattactga aaccgttgaa tgatattggt ctggaacggt caaattcagc
    13021 ttatgacaca tggaaaacaa gacatttttc gatcaattat acgttgaatg gacaaaacga
    13081 attagtcttt tcctttgtat taccgaccat cgatcaacga taccaagtgg aatcgatgag
    13141 tctcctaaac gtatcaccag agacaatgga aatcaatgtc caagatgacc gggtgctttc
    13201 tttgatccga cactggtcag tggatcgagt attctctgca gggcagatca cgatattcaa
    13261 tcacaaattg aatcaattat tagcccatgc aagaacactt ggttttaccg ttaatcagac
    13321 attactagac aatacgaagc cacttcgttt gaatcttcag agcgaatttg aattgacgaa
    13381 tgaagtattg gatgatattt ttatcgtggc aatgaaaaat ccatcctacg atatggaaaa
    13441 gatcaacgaa actacttatc aagtacgatt agataaagga cagtctttaa cgataacaac
    13501 aaacgaaaaa gatcaaacag tcttgtcgat ctcatctgga gactacccac gatctgtcat
    13561 tgatttcttt atcaattacg aattcttggt cccattaatg gtgagaaagt cctgattgga
    13621 taagttggat taccagatgt cattcagata attctgttcc aaaggacttg cggcagttat
    13681 ccagaataat tgggttctat aatattttgt gttggtggaa aatcgaaagc gattttctac
    13741 taacactttt ttcttcgttt caactcaaaa agagactcac ttttggtaaa atttagtcga
    13801 ccaaaacgtc accctaccaa aggagtctct caatggat
//
LOCUS       DAYYME010000070        13259 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000070 DAYYME010000000
VERSION     DAYYME010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.071"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            10..2382
                     /locus_tag="ACHBFW_001743"
     CDS             10..2382
                     /locus_tag="ACHBFW_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFM9318048.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2443..3903
                     /locus_tag="ACHBFW_001744"
     CDS             2443..3903
                     /locus_tag="ACHBFW_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFM9318049.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3914..6118
                     /gene="topB"
                     /locus_tag="ACHBFW_001745"
     CDS             3914..6118
                     /gene="topB"
                     /locus_tag="ACHBFW_001745"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM9318050.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6134..6283
                     /locus_tag="ACHBFW_001746"
     CDS             6134..6283
                     /locus_tag="ACHBFW_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318051.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6358..6951
                     /locus_tag="ACHBFW_001747"
     CDS             6358..6951
                     /locus_tag="ACHBFW_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFM9318052.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6964..7863
                     /locus_tag="ACHBFW_001748"
     CDS             6964..7863
                     /locus_tag="ACHBFW_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFM9318053.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7866..8363
                     /gene="ssb"
                     /locus_tag="ACHBFW_001749"
     CDS             7866..8363
                     /gene="ssb"
                     /locus_tag="ACHBFW_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM9318054.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8729..8989
                     /locus_tag="ACHBFW_001750"
     CDS             8729..8989
                     /locus_tag="ACHBFW_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318055.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9434..9640
                     /locus_tag="ACHBFW_001751"
     CDS             9434..9640
                     /locus_tag="ACHBFW_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFM9318056.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9661..9891
                     /locus_tag="ACHBFW_001752"
     CDS             9661..9891
                     /locus_tag="ACHBFW_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318057.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9907..10344
                     /locus_tag="ACHBFW_001753"
     CDS             9907..10344
                     /locus_tag="ACHBFW_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318058.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10337..11044
                     /locus_tag="ACHBFW_001754"
     CDS             10337..11044
                     /locus_tag="ACHBFW_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318059.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11208..11408
                     /locus_tag="ACHBFW_001755"
     CDS             11208..11408
                     /locus_tag="ACHBFW_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318060.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11424..11603
                     /locus_tag="ACHBFW_001756"
     CDS             11424..11603
                     /locus_tag="ACHBFW_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318061.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11928..12191
                     /locus_tag="ACHBFW_001757"
     CDS             11928..12191
                     /locus_tag="ACHBFW_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFM9318062.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12185..12535
                     /locus_tag="ACHBFW_001758"
     CDS             12185..12535
                     /locus_tag="ACHBFW_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM9318063.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12559..13173)
                     /locus_tag="ACHBFW_001759"
     CDS             complement(12559..13173)
                     /locus_tag="ACHBFW_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9318064.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         4904 a         2194 c         2728 g         3433 t
ORIGIN      
        1 ggaaagaaca tggcagaaga aaaagaacca ctagacaata gtcgtttggg aaaatccaaa
       61 agaaaactag tacgattgca aaatgaatta aacgaacaaa ttgaaaagat gtttgaacac
      121 caaaaaatga cgaacggtca accaatgaac gacaagagga atggccgttc atggtttaac
      181 cagcaagaac gaatcgaaaa taaagtccaa agtctgagag aagagatcaa gcaacaagaa
      241 gaatacgtgg aaaagcttga aagacaagaa gagctaaagg aaatgggcta taacaagtac
      301 ggcggactgg atatgacgat cgataatatt ccaagaatca aagaagaaat cgaacgcttt
      361 gaaaaaggtg aatcggcttt ttcagccgcc acgatccgaa aataccaacg gaagttggaa
      421 acattagaac agctgaaaga aaaatcagaa aaaggaaaag aaaatatcct ccctgaagta
      481 caagcgatca ttgacagcgg acgagtgacg caatggaaaa aaaatccaac catttatttt
      541 ttgaaaggct atcgaaaagt tgctttagaa ctgaatgaaa acgggggatt tgaggaatca
      601 aaaaaatacc aagccacgtc ggaagaggaa aaagcagctg ttcgtgaatt acttgaagag
      661 gctagaccaa ctgattctat gaaggagaaa gaaaacgatc atttctatcg agtagaattc
      721 aacgaaaatg atccagaaaa aggattgaaa agttatggtg gggagatcgt aacattcgaa
      781 ttgatcgatc aactcaaaac attggataac caatacgccg cagaagcagg ttattataaa
      841 ttctattttg aggaagtgaa gaacggagaa gtagtcgatc agatccgaat ggatctaggg
      901 gatggcgtca acagtaatca accaatctat ttagagttag caaaattgtg cgaacaagta
      961 cctgagaagg aacaaaaaca atcggtcaaa aatgaacctg tttcgaaaga acaaatgcag
     1021 tcgaaagaaa caaaaacaaa acaaaagggc tatcaaaaac gaacggcaga agagattaaa
     1081 caagaagtta aatccctttc gcaacaggca ctggatgcgg tgaaaaagca tacccattct
     1141 cctgaagacg tcaaagaact tttgaacttt atgtctcgtt ttccagaacg atcctttaga
     1201 aatcaattat tgattgagga acaatttcca ggtgcaacgg cttgtttagg tcgtgccgcg
     1261 ttgaaaaaag aaggaatcta tatcaaaaaa ggggaacaag ggaacaagat ctttgtcaga
     1321 aaaacggtca aagggtttta tgagaaagga cgtggctttg ttcgagaaac agaagctaca
     1381 ccagaagata aaaaacgaat cgaaagtggt caaatcgaag tgatcaaaaa accttactat
     1441 acgattgaaa aagtgtttga tatcacacaa acacagttaa aacctgaaga ctatccgaaa
     1501 atctttccga accgtgtctt tgattttcaa ttggataaaa aaggacagtc agaacttcaa
     1561 gctgggattc aagcggtagc caacaatatt ggaatacaaa ttcgagatat gacagaaagt
     1621 gaagtctatc aaagagaatt aggacaagca cgtggggcgt atgttcgtaa cgaatttact
     1681 ggcgaagaag aaatcgtcat gaatactcga aatactccta cgcagcattt agctacaagt
     1741 attcatgaat tagcccatgc aagaatgcac aaattttcag aattagatac cgcaactaaa
     1801 gaattgcagg cagaaatgac ttcttatatt gtgtgcaaac attttggaat ggacacgtcg
     1861 gagaaagcta ttccttatat tgcagcatgg acgaagaatg gtcagacgtt ggatgataag
     1921 gaagctgcgg aacgtgggaa aatcatgaat gatgtcagcc gtgtcgcaaa tgaattcatt
     1981 caaaccattt ctaatgaaat caatcaatac agagaaagag aaccagagat ccaaccagca
     2041 gaagtgaagc aggagcataa aaatacttta gaaacgccag agaataagaa tattgtgccc
     2101 gttggttcac tttggatcga caagaaagac gggaaagtaa tggaagaaga tacgggtcga
     2161 acgttacatt tgaaggatgc ggcgtttatt gaagatccgc agcataatca agtaatttta
     2221 aaaggaaaaa gttatggaaa agagatccaa acggcgttac cttcagaaga atttgcgcta
     2281 ggaaatccat tccgattttc acttgagaca gtgtcagaag tggcaaaacg aaaaaatgaa
     2341 atctattatg aacaacaatt gtcacaaaaa gaacgtgtat aaaacaagta aaaaagacta
     2401 tctgaaaaac ggatagtctt ttttcttttg ggaggtttta aaatgaccga gaaaaaaatg
     2461 agtcttattg atcgatgtaa acagatcgat atagtggatt ttgctcgaaa caatggaatg
     2521 gctgtagtca ataaaggacg agattatcgc ttagaagacc atgattcatt tgtttttgat
     2581 cgaagaaaac aacgttttta ttggaacagt caaaatatta gtggcgacat tattgaatta
     2641 gctaaacttt ttttcataga caaagagatc caagacccta agcaacaatt taaagcagcc
     2701 actgatttta ttctaaagaa tgaagacaaa acagaacgag tggaaaatct tcattttgaa
     2761 acagaaaagt acaaagatca ccccgttgat tatcaaccac tcacagaaaa aggaaggaat
     2821 tatctgaagg aagaacgaaa actaccagag tggctgattg attatgcaga aaaagaaggg
     2881 ttgattgccg aactaaagcc aaaatatgaa cggcaaaatt tcttagttcg agacgatcgt
     2941 ttagatcatg cggtcgcctt tttatggaaa gatccacaga caaaagagac agtaggggcg
     3001 agttatcaag gaacgttcat tgattatgaa cgttttggtg agagaggcac atataagcat
     3061 atagataaaa attcgacagc gaatcatggc tttaacctaa agatcggtga ccctaagcag
     3121 ataaaatttt ttgagagtag tattgattta cttagctatg ctgcactgaa tcgtgatcag
     3181 ttaaacgata cgtggttagt ctctatggaa gggttaaaac accatgtgat cagtcattat
     3241 tttggtgagg cagtatctga attgagaaag aaacaagcct ttcctcaatc catcgagatt
     3301 tgtgtggata atgaccgtgc aggacatatt ttctacgaaa aagaacaatt aatgggcgca
     3361 gtcgatccat ttacgaatca gaaagtacgt tgtgaacgag gaatcgctaa tgattggcaa
     3421 gtgccaaaag aatacaaaat catttatgaa gaagtcgcaa aagaagaaaa ggtcacacca
     3481 gaagctatta tggccataca caagacagaa aacaatctgc aattaacgaa ccaactggtt
     3541 tccgctcaca aagtaaatgc gttttttggc cagcagcttt ctgttaacga ttcaatagaa
     3601 gccattaatt tgaaagatat ttgtagagaa gtcgcaaaag aattaaaagt ttgtgaacgc
     3661 gtggatggta cgtacgattt tgatcgtttt tatcaagaaa aaggggatat taatgcgcag
     3721 attcttttct cttataaagc agaacaatac tacaaaggct ataaaaatca tgaacatgaa
     3781 tttgtacctg aagtaaaaaa ggactggaac gatcaattga agcatgagat ccagcaacaa
     3841 gaaatcagaa agcaaaaacg tgccatgttg tttcaacaag gcagacaaca agaaagggag
     3901 tgaaatcgtc atgatgaaaa ccgttatttt agcagaaaag ccgtcacaag cgaaagctta
     3961 tgccgatagc ttctctaaag ccactcgaaa agacgggtat tttgaaatcc aagatcgatt
     4021 gtttcctggt gaaactgtga tcacgtatgg ttttggtcat ttagtcgaat tagattcgcc
     4081 cgatatgtac gatgaaaatt ggaaacaatg gtcattagaa catttgccta ttttccctac
     4141 acattatcac tatcatgttc ctaaagataa gaaaaagcaa ttcaaggtgg tgaaacagca
     4201 acttcaatcg gccgacacca ttattattgc gacagatagc gatcgtgaag gggaactgat
     4261 cgcttggacg atcattcaac aagtaggcgc cgatcaagga aaaaccttta aaagactttg
     4321 gatcaactcc ttagaaaaag aagccattta tcagggattc cagcagttac gagacgcaaa
     4381 agagacctat ccaaaatttg aagaagcaca agcacgtcag atcgccgatt ggttaattgg
     4441 gatgaacggt agcccactgt atagtttact cttgcagcaa aaaggcatac caggaagctt
     4501 ttccttaggc agagtccaaa ccccaacgct ttacatgatt tatcaattgc aggaaaagat
     4561 tagaaatttc aagaaagaac cttattttga aggaaaatcc gaagtaatcg ctcaaaatgg
     4621 cgcatttgat gcgaagcttg atccgaatga aacccaagcg acgcaagaag catttgaaga
     4681 ttatttaaaa gaaaaggggg ttcaattggg aaaacagcca ggcatgattc gccaagttga
     4741 aaccgagaaa aaaagcgcgg caagtccacg tcttttttct ctatccagcc ttcagtctaa
     4801 aatgaatcag ctcatgaaag ctagtgccaa agacactttg gaagccatgc aaggcttgta
     4861 tgaagggaaa tatctaagct atccgcgaac ggatacccct tacattacgg aaggggaata
     4921 tgcgtatctg ttggatcact tagacgaata caagcatttt ttgagggctg aagaaatttc
     4981 gactcctatt catacaccga atagtaggta cgtgaacaac aagaaagtcc aggaacatta
     5041 cgcaatcatt cctacaaaaa cagttatgac agaagcgacg tatgaaagtc tgacaccttt
     5101 acaacaagcg atttacgagc aagttttaaa aacgacagtg gcgatgtttg cggaaaaata
     5161 tacctatgaa gaaacaacga tcttgacgca agtccagcag cttcagttaa aggcaatcgg
     5221 aaaagtaccg atagatctag gttggaagaa gttatttggc aaagagagcg acggaaaaga
     5281 gaaggaagaa gagccgctac ttcctaaagt gacaaaagga gaaatggtga cggtagatct
     5341 acaagtcctt gaaaaagaaa ccaagccacc acaaccttat accgaaggta cgttgatcac
     5401 tgcgatgaaa acagctggga aaaccgtgga tagtgaagaa gcgcaatcca tattgaaaga
     5461 agtggaagga atcggtacag aagcgacacg ggcgaatatt attgaaacct tgaaacaaaa
     5521 ggaatatatc aaggtagaga aaaataagct tgtggtgacg aataaagggg ttttgctttg
     5581 ccaagctgtg gaaaaagagc cgctgcttac gagtgccgaa atgaccgcca aatgggaaag
     5641 ctacttgtta aagattggag aacgaaaagg aacacaaacc acttttctag ccaatatcca
     5701 aaagtttgtc tctcacttat tggaagtcgt cccaggacaa atccaatcca ttgattttgg
     5761 atcgacgtta caagaagtga aagcagccag tgaaaagcaa gaagccgcgc gtcatttagg
     5821 aatttgtcca aaatgccagg aacaagaagt cttactctat caaaaggtgg cagcttgtac
     5881 gtctgaagcc tgtgatttta aattatggac aacgattgcc aaaaagaaac tcacagcgac
     5941 acagctgaag gagattattc aaaatagccg aacaagccaa ccagtgaaag ggttaaaagg
     6001 tcagaagggt tcgtttgaag caacgattgt tttaaaagaa gacttcacta caggctttga
     6061 attttctgaa aagaaaaaaa cgaacttcaa gaaaagaaca cgacgcacga caaaataatg
     6121 gagggttaat cttatgcgta atacaacaca caaacaacaa gaaatcgaaa atcaaaaagc
     6181 caaaattcgt tcagaacaag agaaattacg tgccagtaaa agaagacttc agcaattaaa
     6241 gaaccagcaa cggattcaaa aagaaatcga attagatcga tagacattaa ccttgttaca
     6301 aatacactat catgatatat ttaatgtagt gtatttgtaa caaaaggggg gaagtaaatg
     6361 caagagaaac tgaaagaatt gttggctatt cgcgatggaa atttatctat gaaggaagcc
     6421 agaaaattag gaatcgcagc aaccactgta caaagattgg tgaatcaagg aaaattgatc
     6481 aaagttgatc gtggttatta tgtagaggat ggtcatggaa tagatgattt attttggctt
     6541 cagcaacgtt tttccagggg aatcttctct catgaaacga ctttagatat ttaccagctt
     6601 tctacgaata tgccgaaatt cattcactta acttttcccc atggatataa cgttgatcgt
     6661 tcaattacaa aagagcagca gttacaattt catttcgtga agaaagaagt ttatgaacta
     6721 ggaaaagtag agggaacttc ctttcaagga aatcctatta ctatgtatga taaagagcgt
     6781 acactttgtg atatttggaa tccgagatat gaaattgaat atgaaatgaa attagaagca
     6841 ttaaaagagt acatggaaga cccattgaga gatccaagta aattaagaga ttacatgaca
     6901 aaactacatg tagaaaaaga aatgaaatac cacatgcaaa gcttatattg atggaggaaa
     6961 aagatgacac cgacacaatt tgcagcaaaa acaagaaatt tagcaaagag gacaggatta
     7021 aatgcacagt tagtacaacg gcattttatg atggatcgtt taattgaaca aatttctgaa
     7081 tctaagtata aaaatgattt cattttgaaa ggtggctttt tattaggatc aaaatatgga
     7141 atcgatagaa ggacgacaat tgatatcgac acgacattta gaaatagcaa attaactgaa
     7201 gaaaaattga agagtatttt taatgattta actaggaaac ccacgaaaga aggtattgtt
     7261 ttctctattc aaggaatgaa ggaaacaaga gaagccgatt tttatcctgg atttcaaatt
     7321 aaattggtgg ctaatttaga gaaagtacgt gtgccattta aaatggacgt gacaacagga
     7381 gattctattt atcctgaagt agatacacac tatcaccaat taatgtttga agataaaaag
     7441 gtggagattc cagcttatcc aacagcacaa attattgcag aaaagctaag tgctacgttc
     7501 agctttggca cagataattc gagagcaaaa gattactacg acttatttac cattccgaaa
     7561 ctagaaaata tcgatacgaa agagttatac aaaagtgttc gaaatacatt tacaaaacgt
     7621 ggggatacaa aaccgttgtc cttttattat gaaagagaaa tggataagat cataggaaat
     7681 gactatttgg aaaagcagtg ggaaaaatat cgctcagtta acacgtttgc gaattccatt
     7741 tcatttacag atacagtcaa cgaaattgat cacctaatga aaagtgtgat caaaattgaa
     7801 aatcaagaaa gaaaacggca gtttaaaaga tcacaacaaa tgaatcaaga aatggagaga
     7861 taaagatgat caataatgta actttagtag gacgcttaac caaagatcca gatttacgtt
     7921 ataccgcgag tggtactgca gtagcgactt ttaccctagc tgtcaaccga aatttcacga
     7981 atcaaaacgg caatcgagaa gctgatttta ttaactgtgt gatttggcgc aaaccagcag
     8041 aaacaatggt gacattagct aaaaaaggta gcttgattgg tgtagttggg cggattcaaa
     8101 cgcggactta tgacaaccag caaggacaac gtgtgtatgt gacagaagtt gtggccgata
     8161 atttccaact attagaaagc aaagcagcga cagaaagccg tgcgcacgct gatcaatcgt
     8221 ccaccagtcc aagcaccacc acttttgaac aacgggatac agcgacgcct aataacaatg
     8281 gattgaacgc tttacagaat ccatttggga atccatttgg tggacaatca atcgatatta
     8341 gcgatgatga tctaccgttc taagtagcat tttctattgg ttgcatttgc tacggtttat
     8401 gttataattt agtcaacaaa aagaattttc aatctataac caaaaaccac tagctggcgg
     8461 gctagtggtt ttcttttacg tttctattat aagaattaac gggtaggctt actgtctttc
     8521 ttggcaaatt tacaatcaag ccatttgcag atatagtgcg cggctacttc tgccatgatt
     8581 gcaaaaacaa aatcaaaaag aattttcaaa cctataacct cctccccttg ccagattagg
     8641 ccggggtagc agacagcaag actattatag cataattgaa atatccaaat gattggattt
     8701 ttgctataat tttagcgagg tgattgtgat gaacaaatat gttggtttgc ttgataaaat
     8761 ccgagtaata aaaacgcaac cgttattggt tcgctttact ttacaaacga ttcatgaatc
     8821 gattaattgt gtagttgcag atattgaaat tatcgataaa ttgctaatta tggatgatgg
     8881 aaaatacaat attgccgtca caggtcactt caataaacga aatcagctag ttattgctag
     8941 catgtatgtt agaaatcccg atcattttac gcgatcgatg ggcatataaa aaaagaagag
     9001 ttgcaatcct aatgtaggac tgcagctctt tttgttttac atagaaacaa gaaaaatgaa
     9061 aaggcctttt taggaaggcg cgagcgcctt ctcaattgaa aaacctctct cactccaacc
     9121 gccaccgccg ttcgagaaga aaggaagtga acgttgcacg ttcactaaga taaagggtct
     9181 tgcaaagcaa gacaaaatag aaaaaacttc ctctcaccaa aaggttcgtt cctgttcgtt
     9241 atctctttat tgatttgcca gaccggcaac aagttgcctt ttatcaccaa aaagcgacaa
     9301 ggacaagggt aaatggcacg ctgcgcgccc ttgtccttgt cgcttttcgt tgattcattc
     9361 tggccaacaa atcaataaag aaagaactaa cgaaaggaag taaaaccttt cctaagagga
     9421 ggcagaatca atcatgcgaa agaaatttga ttattggggt gttcccttca gcgaattatt
     9481 cccaaactat catgcaccac atactgtcga atgcgattgt ggacaaagag cgaaatgtat
     9541 caaaagctat cgtttgtatc aatgtccaac atgcggaaaa aaatacacct tgtcttatgg
     9601 cgattatatt ttgattgatg agaaaggaaa gaaacgctga tgaaaacata acataaatta
     9661 ttgactgtaa aagaagtcgt tcaaattttg gtggacaaag aagtcatttc tgaaaaagta
     9721 gcagaagctt tacttattcc gcggtactgg acgatagaaa cccctttagt agaggatgca
     9781 ccgactatgc tatttgaaga cctagtgaag attgcaggat atgaactgca ttatcaagaa
     9841 aacatggaaa cttatgaaaa cgaatattgg ttaaccaaat tagagaacta aaaaggagaa
     9901 aaaaatatgg ctcaaaatta ctatgacgaa tttgtcaaat tacctttgga taaaatggca
     9961 caaaaaatgg aggatatgac attcctatac catgagacac gtgttccaaa aaaacattat
    10021 aaggaaagat tgtcggtagc tgtggaagaa atgattgaat caggtgtcga aatgaatctc
    10081 attgcgacgt actatcgaac actggaagaa ctgaaaaaac aaaatgcaaa atggtttttc
    10141 caagccttac tatgcttgga agtaggtgtc aaaccaagca ctattaaacc ttctgaatat
    10201 caagctttag aactaacgta tgggaaattt gtcgaaacga aaaaagccaa gactgtttca
    10261 agtgaatggt tagattattt tgaaaatatc aataagtatg gcgcctatta caccatgaaa
    10321 aaggaggaca acgaaaatga ataaggtgat ttatcaacta acaacggaag atgtgtcaca
    10381 ctttttaaca aacaaagaac atttgaatga agtaatcaga cggattcaaa aagactttag
    10441 ttgtatggaa gaaatgaagt actttattca aagagtcgaa gaacaattgt atttggaaag
    10501 tgaaacgagt caaacgaagg tcacagtcag caataattat ttagacgtgt ataaacatcg
    10561 tcctgaaacg gtaaaaaaag gaacggagct tctacgacta gaaacagaaa aatacatcat
    10621 gaatgtttac ctagaaagga gtgaactggt cgaaaacttt gtcagctgca cgatttgcag
    10681 gaaacactat tactcagaat cagaatatta ttcaattttt gagagcatgg aattaatgga
    10741 agaagaaatg gaagagctaa acagcaaaaa tgcgacgata gacgattatt atcttgtatt
    10801 tactagattt ttgacgtgtg gaacggaatc tatcgtgtta atgggaatgg attatgaact
    10861 cgctgtaccc gaagcatttt tgagggaata tgtagaagaa gtagataacc caggcgacga
    10921 ctggtacacg actttaactg attttttaga tacctacact tacgacgata cagaagaaat
    10981 ccaaaatgaa cttgaccgta aacgaatgcc atatcaacga tttgaaagga aaaaaaggca
    11041 ataatcattt ataccgaaaa gtgagaatgt gtgaaataag aacaaaaacc ttgaaattaa
    11101 gggaatggtt cttatttcgc accaatcgct ccaaccgccc ccgccgctta ggtggtgcga
    11161 aataagcggg caaagcccga taacctaaga gaaagaagga gatccatatg aataaaatca
    11221 ttcctctgtt gttcgtactg tgtattggcg agatacttat gcgtcatggt gcagctgtac
    11281 atttaaactt cgtagtattc ttaggaatac tagtattttg tttgattttt tggcgctttg
    11341 tgttagccct tgtgagtcta ctattcaagt tgattgcagc catatttttc atagggttat
    11401 ttcgatgaaa ggagagagta ctaatgccaa ttgcaatagg aaataaacgg ttacccgtga
    11461 cattagatga aaagagacaa aaagaattgc agcaactaaa gcagaagtac ggaaaaagtg
    11521 aatctaagat tatgtgtatt gcgttagatc tattgatcgc gcaagaaaaa gcaggatttg
    11581 atataccagc actaaaaaag tgacgtcact tttcatttga agaaacaaaa gtgatagcac
    11641 ttaattgttc ctactattta gaatattttc tatgtccttt ttattccatt ttgtatcaat
    11701 aactatgaaa tctttaaagt atccagatca atttttttct tacctgattc taaagatttt
    11761 gttttcttaa acatattatg atttcctttc gactattttc atataaatgt taacatagtt
    11821 tatggattca tggaatgtta atctaattta atagaaaagg ataattaagt atatacgttt
    11881 tttcttatgt atgttatact atgtatatac agaaagaggt gtctactatg gaaatgttac
    11941 ctataaaaaa atggggaaat agtaatggcc ttagattgcc aaagtatatt atggaatatc
    12001 ttggaattca tacagaagac aaagtgaaga taatacaaga agaagtaaac ggtcagaagc
    12061 ggctaattat cgaagcggct aatatagaaa atgaattaac aattgaacag ttatttgaaa
    12121 attacactga tgaaagaaat catgtgacga ttcaaaattt aggtgaagca gtaggtaatg
    12181 aaaaatggtg agaaaaccta gacaaggcga tattctatta ttgaatacag cacctcgatc
    12241 tggacacgaa cagacaggta aaagacctta tattgtttta agtcatgata ttattgcgga
    12301 ttatagtaac gttgttacag ttgctccaat ttctagtaca actagaaact atccgctata
    12361 tgtaaatata aactcagaat atgaaatgaa gacaacagga aaagtattat tagatcaact
    12421 aacgacaatt gattatgaag ccagagagtg tatttttttg gaaaaagcgc atgatagttt
    12481 agttgaggag ttattgatga aagttagaac agtttttcaa aaagtaagca aataaaatag
    12541 aaacagcagc atgatcaact attcgtgatt gaccatgctg tcttttttta ttctatatac
    12601 ggtttgtctc gtaacattgt agtcttttgc aatcttagcg atcgcaacac ctttttttag
    12661 atcctcaacg atgtttttat aaacaagacg acgttgggga tcttttgcat tggcactgta
    12721 tagcttagga cgtcctttat aaacaccgtt cgcttttgca atcttaatgc cttgtgcctg
    12781 acgtcgttta gattcggtac gttcttgttc agcgatcatg gccaaaattt gaatgatcaa
    12841 atctttaatg aatttatcca ggagaggatt gccaattgct tcggccatga tcggtagact
    12901 tgtgatgatc agctgcacat ttttcttctt caagtaattg actgaagcga tgatctcgtc
    12961 gtaatttctt ccaagtcgat caatagcttc cacaataaaa atatcttgat ctcggacgaa
    13021 atccagtgct tcttgaaaaa caggtcgatg agtaatcgtt gcgccagatt gtttctcaat
    13081 gaaaattctc tctgcaccga attttttcat ttcttcaatc tgccgttgtt cattttgatc
    13141 gatggatgag acacgtatgt atgcaactct caaaattttt acctactttc tgcaactatt
    13201 taaaaaacac cctaatcata cacgagaaaa ccagattttt caatgtcgcg gtcgtgtct
//
LOCUS       DAYYME010000071        13141 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000071 DAYYME010000000
VERSION     DAYYME010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.072"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..753)
                     /locus_tag="ACHBFW_001760"
     CDS             complement(109..753)
                     /locus_tag="ACHBFW_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9318065.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(847..981)
                     /locus_tag="ACHBFW_001761"
     CDS             complement(847..981)
                     /locus_tag="ACHBFW_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFM9318066.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(983..3115)
                     /gene="feoB"
                     /locus_tag="ACHBFW_001762"
     CDS             complement(983..3115)
                     /gene="feoB"
                     /locus_tag="ACHBFW_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFM9318067.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(3112..3585)
                     /locus_tag="ACHBFW_001763"
     CDS             complement(3112..3585)
                     /locus_tag="ACHBFW_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFM9318068.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            3986..4210
                     /gene="nrdH"
                     /locus_tag="ACHBFW_001764"
     CDS             3986..4210
                     /gene="nrdH"
                     /locus_tag="ACHBFW_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFM9318069.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            4369..6528
                     /gene="nrdE"
                     /locus_tag="ACHBFW_001765"
     CDS             4369..6528
                     /gene="nrdE"
                     /locus_tag="ACHBFW_001765"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFM9318070.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            6578..7543
                     /gene="nrdF"
                     /locus_tag="ACHBFW_001766"
     CDS             6578..7543
                     /gene="nrdF"
                     /locus_tag="ACHBFW_001766"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFM9318071.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(7632..8771)
                     /locus_tag="ACHBFW_001767"
     CDS             complement(7632..8771)
                     /locus_tag="ACHBFW_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9318072.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(9080..9793)
                     /locus_tag="ACHBFW_001768"
     CDS             complement(9080..9793)
                     /locus_tag="ACHBFW_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFM9318073.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(10047..10748)
                     /gene="nagB"
                     /locus_tag="ACHBFW_001769"
     CDS             complement(10047..10748)
                     /gene="nagB"
                     /locus_tag="ACHBFW_001769"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFM9318074.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            10982..12514
                     /locus_tag="ACHBFW_001770"
     CDS             10982..12514
                     /locus_tag="ACHBFW_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9318075.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(12705..12884)
                     /locus_tag="ACHBFW_001771"
                     /pseudo
     CDS             complement(12705..12884)
                     /locus_tag="ACHBFW_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(12904..>13141)
                     /locus_tag="ACHBFW_001772"
     CDS             complement(12904..>13141)
                     /locus_tag="ACHBFW_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9318076.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         4199 a         2654 c         2314 g         3974 t
ORIGIN      
        1 tttgtcaaaa atgaaagaaa tttgcttgtc caaactgaaa aaaacttggc taaatctttg
       61 taaataaaag gtttagccaa gttaatattt ttttaatatt tcacacaatc attctgcaag
      121 ctgatgctcc gggtaaaaaa agtttgtcat acgctgtagt gtatattcat ttccaataaa
      181 atgaacgatg tcccctgccg taaatttagc ataggggcca ggtgaaacaa gcagttcttc
      241 attatgagaa attgcgataa ctgtggcgcc agtcgcctgc cagatattca actcgctaat
      301 tgaacggtcc agatgcattg ctttttctga taaagataat tcaaacggaa gtaaaggatt
      361 catcttatgt attcgcttcg tttgattgac cagctgatcc aataattgag aaaaatgatt
      421 cagctcatct tgttgcttct ccacacttgc catgatttct gcttttgtat cttggatgga
      481 ttgtacatct tgatattgca ataggaaatc ttttgctttt tccttcgaag caacaaatgc
      541 cccacttcca tgccgtactt ccatgattcc aagatctacc agcacattga tagccttgcg
      601 ggtagtctca ggagaagcat tgtacgtact ggcaagtgtt gagcgggcat ggatcttctc
      661 acccacttta tatcggtttt caacgatacg ttcggctaaa tcaacagcaa tctgctggta
      721 gcgcgggcgg acgatttttt tctttccaga cattttcttt cctctttctt tttttacttt
      781 ccctcatctt actatgctct agcaaaataa gcaaaaaagg ggagaatagc ctcttgcaac
      841 gactgatcat cgcttgatct gttccttttt tactgggcaa gctgtttgac agtcatcaca
      901 attcttaccg gattttaaac gagtatagac aattgcgcct gcacttccaa agattaacac
      961 acttaataag aatgtcgcca tcctattcct ctccttctaa tgacaatata gtataaaact
     1021 ggttcttgga ttttggctga cggaacaaaa cataaacgag tcctatcatc agtaaaatag
     1081 caagatacgt ccctaacgct acctgtccac cctccaataa gtgtcccaac tgataaatca
     1141 caaaactaac taaataagct aaaccgcatt gatagcctat ggcgatccaa gtccatttct
     1201 tggcattcat ttcccgatgg atagccccaa ttgccgcaaa acaaggcgca caaagtaaat
     1261 tgaaggctaa caatgaataa gctgctgcgg gagtatatgt gtgttgtagc acttgccaga
     1321 tttctctgcc attttcggat acttccgcat gaccaaacaa aataccaaat gtgccaataa
     1381 tattttcttt agcgaccaat cctgtaatcg ctgcgactgt tccttgccag tttccccaac
     1441 caagcggctg aaaaacaact gccaggattc gcccgaaaaa ggctaagata ctttggtctt
     1501 cagaaacagg ttgagcataa aaattataag aagagctgaa ccagatcacg atactagaaa
     1561 caaagataat cgttcctgct tttttaacaa aagacttgct gcggtcatag gtttgtctta
     1621 ggaccgctga ccattgagga aaatgatatg cgggcaattc catgataaaa ggagctgtat
     1681 ctccagcaaa caaacgtgtt tttttcaagc tgattccaga gaaaataatt gcagtcatac
     1741 caagaaaata ggcagaagga gctacccaag attgatgagg aaaaaaggcc cctgctacta
     1801 aggcaataat cggcaacttt gcagaacaag gcatgaaggt tgtgaccatg atcgtcaaac
     1861 gacgatcttg ctcgttttca atcgtgcgac tggccatgac gcctggcact ccacatcctg
     1921 tagcaatcag cataggaatg aaggattttc ctgaaagacc gaatttcctg aaaagtcgat
     1981 ccatgacaaa agcgatcctc gccatatatc cgcagtcttc cagaaagctc aagcataaaa
     2041 acaatataat caattgcggt agaaatccta acactgctcc cacgcctgcg atgatgccgt
     2101 tcaagatcag ctcctgcatc catcccgcta cttgccattc agctaacaat ctgccaacat
     2161 gttctggtaa ccatgtacca aacaatgtgt cattgatcca atctgtcccc attgttccta
     2221 ctgtttgaat agccagataa tagatcagcc acatcacaaa tgcgaagatc ggcaaggcta
     2281 accaacgatt cgttgcaatc tgatcgattt tgtcactcat cgataattgg aaagaatcat
     2341 ttgacacaac acagagtgta catagttggg taataaattc ataacgttca ttgaccagaa
     2401 tcgtctcact atcgtcgtcc aatagtttct ctgttaactg gatcagttgc tcgatctctt
     2461 tttgctgaga aactgctatc tcgtaatcct ctcttacctt ctgatctcgt tcaaaatatt
     2521 tcattgcata ccagcgtttc ttgccgcttt gttctcctag tacatcttca atttcatgta
     2581 aagctgcttc taaacgtgga tcatatgtac agggaacagg aatagaaggc cgcttttttg
     2641 cagactcctg aatcttttcg attagttttt cgattccttg ctttttcaat acgctgattg
     2701 gtaaaactgg tacaccaaga ccataggaaa gcttttctag attgattttt tttccttctt
     2761 ttttgatgac atccatcata ttcagaccaa tgatcatagg aatgtctaaa tccaataact
     2821 gcgtagttaa ataaagattt cgttctaaat tggtagcatc aataatgttg atgatcagtt
     2881 caggctgttc ctgaaccaaa taatctcttg caatcacttc ttctggtgta taaggagaca
     2941 aagaatagat gccgggtagg tcctgaatat gaatgttcgg attttttttc aacgatcctt
     3001 cttttttttc tactgttacg cccggccaat tccctacata ttgattcgaa ccagtcaaga
     3061 gattgaacaa gctagttttc ccgctgtttg gatttccgac taacgcgata ttcattcttt
     3121 ttcctccctt tgtatcaaga ttagttcggc ctctgacttt cgtaacgtca gttcataccc
     3181 tcgcactgtc agttcgattg gatctcccag aggggctagt tttcggataa ctatttttac
     3241 atttcgcgtc aatcccatat ccattaaccg tcgcttcact gcgccttgtc cgtggattgc
     3301 tacgacgatc ccagtctctc ctgcttttaa ttgatcaaga gaagtccagc tttcagagga
     3361 agcagtcttt ttattgacaa agatttgacc tagtatttcc cgacttactg ccactcgatg
     3421 actttttacc agtaaaatgg cattgttgcc tgataaattg acgacgacga ttttacttcc
     3481 ggcaacgatc ccgatatttt gcaaatggcg ttttgttttt tcttctgttt cgattcgttc
     3541 gacttgataa acgaaattct gctttgcctg cgctaaggtc tgcatattga ttcctctctt
     3601 gtgaaataaa tcatgtatta aaatcattca ttatatgttc taattttctt acgtgttacg
     3661 caaataaatg agaatcattc tcacatagaa tactctcggt aattgagaat gtcaattata
     3721 cttgtgatat tatgagcgaa tctttagtat tgatagataa aaatgagaaa aaaatatttc
     3781 ttcatagttt tccacaattt tttctctatt aaatttgcta ttttcccaca cgatacttgt
     3841 tttcagctct tttttcaaaa acacaaaact tagtattcca cagtttccac aacacaatat
     3901 gtagtgtttg ttgtttgact tttttactaa gagttttata ctatctatta tagataataa
     3961 gaatgtgtta gaggaggcac tcaccatgaa cgtaaaaatc ttttctaaaa ataattgtat
     4021 gcaatgtaag atggtcaaac ggtttttaac agaaaatcaa attgcttttg aagaagtcaa
     4081 tatcgatgaa cagcctgaag cagtagactg gttgaaagca caaggattcc aaagtgttcc
     4141 tgtcattact tctgatgcag aaacagttat cggattccgt cctgatcagt tgcgtaaact
     4201 tgccagctaa catgactaaa agaatcaaaa cgtgatacat ggatttgaag ggcttgagac
     4261 agcaccagtt tttgtcgcaa acttccaaat ccttttttaa agcttaaaaa caatgcaatg
     4321 atttgcaatc aagcatcatc aaatacgaaa tgaaggttgg gcgtacgtat gagtcttaaa
     4381 aatttacaag atgtcagtta tttcaagctg aataatgaaa tcaatcggcc agtcaatggt
     4441 caaatccctt tgaataagga taaagaagct ttagttgcct tttttgagga aaatgtcaaa
     4501 ccaaatacga tgacttttcc atctattatg gaaaaaatca attacttgat cgatcaagaa
     4561 tatatagaag cagaattcat tcaattgtat tcaccatcat ttattgaaaa gctatatcaa
     4621 tttttggacg agcaacattt ttctttcaaa tcttttatgg ctgcttacaa attttattct
     4681 caatatgcgt tgaaaaccaa cgacggttcc gcttatctgg aaacttatga agatcgagta
     4741 gccttcaatg cgctttactt tgcgaacggg gatgaagaat tagcacttgc tcttgcagac
     4801 gaaatgatcc atcaacgata tcagccagct acaccttctt tcttgaatgc tggacgtaaa
     4861 cgtcggggcg aattggtgtc ttgtttcttg actcaagtca ctgacgatat gaactctatc
     4921 gggcgctcaa tcaactctgc tttgcaattg tcccgtattg gtggcggcgt agggattact
     4981 ttatcaaatc ttcgtgaagc cggcgctcct atcaaaggat atgagggtgc tgcaagtggt
     5041 gtcgttccag tcatgaaatt attcgaagat agtttcagct attctaatca attaggtcag
     5101 cgtcaaggag ctggcgtggt ttatttgaac gttttccatc cagacattat tgctttcctt
     5161 tcagcaaaaa aagaaaatgc agatgaaaag attcgcgtaa aaactctttc tcttggcgtg
     5221 atcgtacctg ataaatttta tgaattgaca cgtaaaaacg aagatatgta tttatttagt
     5281 ccatatagtg tcgaaaaaga atatggtgtc ccattctctt atgtaaatat tacagctgag
     5341 tatgatcatc tagttgcaaa tccaaacatt cgcaaacaaa aaatcaaagc gcgtgatctt
     5401 gaaaatgaaa tctcaaaatt acaacaagaa tctggctatc cttacgtaat caatattgat
     5461 acagcgaacc gcgagaatcc aatcgatggc aagatcatca tgagtaattt atgttcagag
     5521 attttacaag ttcaaacgcc ttctgtgatt aacggcaaac aggaatatga agtattagga
     5581 acagatatct catgtaactt aggctcgaca aatatcgtga atttgatgca aagccctaac
     5641 tttggtcaat ctgtgcgtac gatgactcgt gcattgacat ttattacaga tgcatctgat
     5701 atcgatgtcg taccaccgat ccaaaacgga aataagctga atcatacgat tggattaggt
     5761 gccatgggct tacacactta tcttgcaaaa gaacaaatgg aatatggctc agaggattca
     5821 ttagacttta caaatattta cttcatgctg ttgaattatt ggacacttgt tgaaagcaac
     5881 aatatcgctc gtgaaagaaa acaagttttc cataattttg aaaaatcagc ttatgctgac
     5941 ggcacttatt tcgaaaaata cgtaactgga gaattccagc caaaatcaga aaaagtcaaa
     6001 gcattgtttg aaggcatttt tatcccaact gctgaagatt ggaatgcttt gaaacaagca
     6061 gtaatgaaag atggactgta tcatcaaaat cgcttggcag tagcaccaaa tggctcaatt
     6121 tcatatatca acgatacgag tgcaagtatt catcctatca ctcgcatgat cgaagaacgc
     6181 caagagaaga aaatcggtaa aatttattac ccagcgcctt atctatccaa tgagacgatt
     6241 ccgtattaca cttctgctta tgatatggac atgcgtaaag taatcgatgt ttatgcaacg
     6301 gcacaacaac acgtcgatca aggcatgagt ttgactttat tcatgcgttc tgaaatccca
     6361 gaaggcattt atgaatggaa gacaacaacg aagcaaacga cacgtgattt gaatatttta
     6421 agacattacg ctttccacaa aggaatcaaa tccatttatt acgtacgtac ctttactgat
     6481 gatgcagaag aaatcggaag caatcaatgt gaaagctgcg tgatctaata tcatagatag
     6541 attcgcttat tcatatttta attaaaggag cattctcatg tcagaaacat attatgaagc
     6601 aataaactgg aatgaaattg aagatatcat tgataaatcc acttgggaga agctgacaga
     6661 gcaattttgg ctagatacac gtatcccact ttcaaatgac ctagatgatt ggcgtacatt
     6721 gtctcaattg gaaaaagata cagtaggaca tgtatttggc ggtttgacac tgctagatac
     6781 tgtccaatct gaaagtggca tggatcagtt gcgtaaagat gtccgcactc cccatgaaga
     6841 agccgttttg aacaatattc aattcatgga atccgttcat gccaaaagtt attcttctat
     6901 ttttagtaca ctgaatacga aaaaagaaat cgaagaaatt tttgactgga caaatacgaa
     6961 tccttatctg caaaaaaaag ctgagcgtat caatgaaatc tacaaacacg gtacaccgct
     7021 agaaaagaaa attgccagcg tctttttaga aacattcttg ttctattcag gtttttatac
     7081 tcctctttat tatttaggaa acaacaaatt ggctaacgta gcagagatca tcaaactaat
     7141 catccgagat gaatctgtac atggaaccta catcggttac aaattccaat taggattcaa
     7201 cgagttacct gaagcagaac aagaaacgtt aaaagactgg atgtacaatc ttctttatga
     7261 gctttacgaa aacgaagaac gttatacaga agaattatac gatccaatcg gctggacaga
     7321 agaagtcaag acattcctac gctataatgc caacaaagca ttgatgaacc tagggatgga
     7381 tccactcttt ccagatactg ccaatgatgt caacccaatc gtaatgaacg ggatttcaac
     7441 aggcacaagc aaccatgact tcttctcaca agtcggaaac ggctatttgt taggtcatgt
     7501 agaagcgatg aaagacgacg attatttgat tggattggaa taaggattta ttttaattca
     7561 aaaatttttc gaaaaataaa aagagtttag cgtacagata ttttaaatgt acactgaact
     7621 cttttttatg cttattcact tgtttcttcc agcacttttg gactgtctaa cttttccgaa
     7681 acatgttcca ttcttctcaa taaatagatt ttccggatat ttaatattat cgtcttaatc
     7741 actgcataga caggtatgca aatcagcatg cctaatattc ctgcgacact tcctgctcca
     7801 atcaatacca agatgatcgt caaaggatgg atattcattg atttcccaaa taacaacggc
     7861 ttgatcaaat ttccatctaa ttgttggatc actaaaatcg acaacgccat atacaaagct
     7921 tgaaatggag agataaacaa accgacaatg attgctggta ccgctccaat aaaagggccg
     7981 acatacggga taatattcgt gattccgcaa atgaccgcaa gcaacaagct ataaggctga
     8041 tcgaatattc tcattgccaa aaagctcatt acaccaataa tcaaagcatc taagatcgta
     8101 ctgctgatat aggcagataa tgtttcgttt aattctttta ccgtttggcg taactcttta
     8161 cgaatcgctt tcggaaagaa gtgagacaac gcatcaataa atttgtgccc atccttgaac
     8221 ataaatagca aaataaatgg aacggtaaat aacaaaataa aaaattgcat cataaatgaa
     8281 atgatttttg agacacttgc agataaactg actaatatga tattgaacaa attactaaga
     8341 gataaatttg ctgatgctaa ttgttcttcc aaattgaaat ttttaaactc ttcccgctgc
     8401 gccaattcat ttaaccattt gcttgtttca tctgcataca ttggcaagct ctgggtcaat
     8461 tgaattgtct gttccattaa ctgaggcact acattcatga cagttaaaac aactaaaatg
     8521 attacgacca aaaacgtcaa taaaaaaccg aacactctcg ggatccgatg cttttcgaga
     8581 aaaacaacga ttggatcgaa catataatag ataaaaccag caacaagcaa tggtaaaaaa
     8641 attgccgaaa gcattttcaa tattggacta aaaatattcg gcatttgtaa aataaagtac
     8701 acgccgatga tcgtgagtac tagctccact gtccagaaaa ataattttga ttgtttaaaa
     8761 cgtgattgca tacatctcct ccaaggtcct ttgatacttc tattctatca tttaacaaag
     8821 agttacgcgc tgagattttc atttgcttaa aaaaaacaaa aaaaataaac atattttaat
     8881 gagtagacga gtacttaaac aaaaacacgg tttggagtag tgacaaaaat tatactgcag
     8941 tccctttttc caaataaacg gtgttcaaaa gcttgcatct tcgtaataag cccagccaaa
     9001 cgaaaaatat aaggaactat ttttatttgg ctgggcttat tacttgatgt aggacatcta
     9061 ttttacaacc tcagatcatt tattgcgcaa tactgactaa atgcaacttt gtgatatttt
     9121 tgtgtacgga caactcctcg atcacatcgg cataagtgag cccttttgga agatcaatcg
     9181 tatagatatt tttgtacact cggtagtcat caacgaattt tacgtcaaaa ttcacatctt
     9241 cgattacgat atttttctct tcaaaataat cggtaagaaa atctttggtt tcttttctat
     9301 ggataaattg gatttctaac tttttagtcg taggtacatg gatcacgcgt ttcactactg
     9361 tgagtgcaat agcaatcccg ataaaactga tgatcgcaat ggaatagtaa cccatgccga
     9421 tggcgatccc taatccagca attgcccata aagaagcggc agtcgtaagt cccgtcaccg
     9481 attgtttcgt gacgatgatc gttcccgcgc ctaagaaacc gacaccactc accacttgag
     9541 caatcagcct tgcttcatct gaacgaatcg ttccagcaag ctgtggatac tctaaagcat
     9601 ctcttaatgc accactagcg atctcaactt ggatcaatgc aatgatacat gctcccatac
     9661 aaactaaaat atgcgtacgc atacctgctg gacgattttt atattgtcgt tcaaaaccga
     9721 ttgctccgcc aaatagcata gcgatcccta aacgtaaaat aatctctgga attgtcaata
     9781 ccatatcatt catctaaaca ccctccagtc caatatatca tgtctgaaag gagcagacaa
     9841 aaaaagtgca atttttacct cttacgaaaa aaggactgtg acaaaatttt tgtcatagtc
     9901 ctttttatga ataaacggtg ttcaatcagc tgaccctcgg tattactcaa caaacggcaa
     9961 aatatgggga gctatttttc ctttgtcttc tctaatactc aggtcaaaac gctgatttca
    10021 caacctcttt agcctactat tgaaaattac aacttacttg ctgcttcttc atcgatgatc
    10081 acaacaacat tgtcgtgatt ttgcaataca cttgctggca aatgattcgt tacaggtcct
    10141 tcaatcatcc ctttgattgc atcagctttg ctttcgccaa atgccatcaa gacgatttct
    10201 ttacttttca taattgaacc gattcccatt gaaacagctt ttgttggtac atcttctgct
    10261 ttatcgaaga atcgtttgtt tgcattgatt gttgattctg ttaagtcaac tactgaagtc
    10321 gttccgtcaa atgaagcacc aggttcattg aaaccgatat gtccattttg cccgattcct
    10381 aagatttgga tatcgattgg atgtgcatcg atgatttgat catacttttt gcatgctgct
    10441 tccagatctt ctgcttttcc atctggtaca aacgtttctt tgaatggttt tttatcaaat
    10501 aattggacat ccatgaaatg acggtagctt tgttcatctg aaccgcctag tccaacgtac
    10561 tcatctaagt ttacagaagt catttcactg aaatccaaat cgcttgaagt catttcttta
    10621 tataaagtaa ctggtgtgct tcctgttgcc aatcctaata cttttgcacc tttgttcata
    10681 ccttctttga tgatttcaaa tgcttttttt ccgccttctt cagcgttttt tacacgaatg
    10741 atttccatag tgattgctcc tttatatttt ggtatagccc aattatagca ttcttttttt
    10801 ctttttacaa taattagtat agaccaaatt gcattttttt cattttcttg acaataaata
    10861 ggaatccgtg ttaggtttca taagatggtt caatcaaaca atccatgttg catgaaaacc
    10921 aagcaagtga ttcactgtgg acgctttatg tctgcagttt ttttatgaaa aaggagttat
    10981 tatgagtata ctgacattaa acaacattac ccagcaattt ggcgacaaaa tcctctatga
    11041 aggtgtcgat ctgcaattaa atgctggcga acatcttggg ctgattggac aaaacggagc
    11101 cggtaaaagt acattgatta aaatcatcac tggtgagatc ttaccagatg acggacagat
    11161 ccattggcaa aaaaatattc acaaaggtta cctcgatcaa tatgtggaag tcgatgaaac
    11221 attaacaatc gaagaatttc taaaaactgc gtacgcaaaa gaatacgaaa aggaagaaaa
    11281 aattgctttt ctttaccaaa catatagcga gaccttcgat gattccttgc tggaaaaagc
    11341 tgggaaactc caaacggaat tagaccaagg agtgttttat caaatcgaca ccttagttgc
    11401 tgagatgagc agcgggttgg ggatcgatgt cttaggttta gattcactgt tgaaaaatct
    11461 aagtggtgga caacgttcaa aggtgatttt agcaaaacta cttttagaaa acccagatgt
    11521 tctgatctta gatgaaccaa ccaatcactt agatgatcag catatccaat ggctgacgca
    11581 gtttcttcag gattttgacg gtgcgtatat cgtgatttcc catgaccaag aatttcttga
    11641 tcagatcacc acccatatcg cagacatcga gtttggaaag attactaagt atactggcca
    11701 tctaaaacaa gcattgaaac aaaaggaaca aaatcgcgaa agctatttgc gccaatatca
    11761 tgctcaacaa aaacaaatcg aaaagaccga agcttatatt cgaaaataca aagccggttc
    11821 ccgatcaacg atggcaaaaa gccgacaaaa acaattagat aaaatcgaac ggctgacgcc
    11881 tccagcttca gcaagtaaag ctgtctttga ttttccattc tcgcctattg tcacgacatt
    11941 agcagtggaa acaactgatt tagtcatcgg atatgatcga ccgctacttg cacccatcaa
    12001 tttgaccatc cgctttggtg aaaatattgc cattcgaggc ttcaacggga ttggaaaatc
    12061 tactttatta aaaacactga tcggtgagat tgaaaaaata agtggcgatt tccacttccc
    12121 agataacaca aaaatcaatt acttttcaca agatctgtac tgggaaaatc cattagaaac
    12181 accactacaa tatttaagca gtcaatttcc aaaagcgact atcaaagaat tacgtcgcca
    12241 actagctaaa gcagggttag ttaatcaatt agcaagtgaa cctctcacta cattaagtgg
    12301 tggggaacaa actaaggtga aacttgcaca gttgacaatg aaccaaggta atttactgat
    12361 tctcgatgaa ccgacgaacc atatcgatca agaaacgaaa gaaagcttac aagacgggat
    12421 acagaaattt ccaggaacag taatcatcgt ctctcatgaa caagaatttt atcaagatct
    12481 tgtgcaacga gtcattgaga ttgaaccaaa ataaacgttt gattgaatag ttgaaaaaaa
    12541 ggagctgtga caaaagtttt gtcacaactc cttcttctga ataaatggtg gccaattagc
    12601 tgaccttcgg tattagctca aaaaacggca aaatatgagg agctattttt atttggctat
    12661 tcttattact tgatgcagaa cagcttttca caacctcttt tcgtctattt tttattttcg
    12721 agatttctct ttcctttaaa aacaactgtt tgaaagaaga acgcacatcc ttgaaaaaca
    12781 agcccgctaa ccgttacacc taaccagcca tactgctgcc acattgtcgt ccctagcaat
    12841 gaaccagccg cacctccaat aaagtacata aacataaata cagacccgtg gtgctagtta
    12901 attttaatat cctaaagaat atctcaaagt tccttcaaaa cgtcgttgtg ctcgacttaa
    12961 tagaacactg taagccagta atatgctttc aaatctactc tctagccctt taacagaacg
    13021 tgagttgaaa ttttgacaac ctaatttttc taaggaagaa aaaacagtct caactgtttt
    13081 tctttgtttt ccaagtaagc tgtggtctag tttttctgat ttatccatgt ttttacgtgg
    13141 c
//
LOCUS       DAYYME010000072        13086 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000072 DAYYME010000000
VERSION     DAYYME010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.073"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..2199)
                     /locus_tag="ACHBFW_001773"
     CDS             complement(79..2199)
                     /locus_tag="ACHBFW_001773"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM9318077.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="ACHBFW_001774"
     CDS             2429..3607
                     /locus_tag="ACHBFW_001774"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFM9318078.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="ACHBFW_001775"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="ACHBFW_001775"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFM9318079.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="ACHBFW_001776"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="ACHBFW_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFM9318080.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="ACHBFW_001777"
     CDS             complement(4960..6213)
                     /locus_tag="ACHBFW_001777"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM9318081.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="ACHBFW_001778"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="ACHBFW_001778"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFM9318082.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="ACHBFW_001779"
     CDS             7790..9136
                     /locus_tag="ACHBFW_001779"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFM9318083.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="ACHBFW_001780"
     CDS             complement(9444..9830)
                     /locus_tag="ACHBFW_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM9318084.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10098..10247)
                     /locus_tag="ACHBFW_001781"
     CDS             complement(10098..10247)
                     /locus_tag="ACHBFW_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318085.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10709..10921
                     /locus_tag="ACHBFW_001782"
     CDS             10709..10921
                     /locus_tag="ACHBFW_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318086.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11199..12998)
                     /locus_tag="ACHBFW_001783"
     CDS             complement(11199..12998)
                     /locus_tag="ACHBFW_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFM9318087.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2354 c         2577 g         3864 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
    10501 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
    10561 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
    10621 atgaataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
    10681 aataaaaata aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat
    10741 atgcaaaccg atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa
    10801 cacggctgtc gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag
    10861 caagtatcgc atgctcgtaa aattctgtga tattagcgca cctactgaat tggtttctta
    10921 aacagtattt ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag
    10981 cgagaagata tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc
    11041 tctgcaattc ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa
    11101 gcacacatta tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt
    11161 tttgttgtat aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa
    11221 tttagcagtt tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt
    11281 agtcccatct catcggttat gataccatcc acaccataaa acatcatgcg actcatggca
    11341 tctgaatcgt tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg
    11401 aagttacggt tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc
    11461 ccaacaatat tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa
    11521 tctgaaacgg tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg
    11581 cggtatttat ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc
    11641 tcaataagta atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa
    11701 acaggggctt gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga
    11761 tcctgtggtg ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg
    11821 aaatgaccat cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg
    11881 cttgtttttt caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg
    11941 tgagagacag tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga
    12001 cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag
    12061 aaacgcaagt tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg
    12121 tcatccatga aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat
    12181 aataaaatcc cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg
    12241 aaaaattgtt caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg
    12301 agtaaaatcg taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg
    12361 gtcaatgacc agctttcccg aatggctgct cgaaaagaac gatctcgtag aatcatttcc
    12421 ggtaaagcaa aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag
    12481 gaactgacga tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt
    12541 atttttgaca gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa
    12601 taagcaagga aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag
    12661 tgtaagagtt gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa
    12721 agaaaagtaa attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag
    12781 agtagaaccc caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca
    12841 attgcccctc ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat
    12901 aaaataaagc catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag
    12961 tcccaagcat tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt
    13021 gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga
    13081 tttaac
//
LOCUS       DAYYME010000073        12963 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000073 DAYYME010000000
VERSION     DAYYME010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.074"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..771)
                     /locus_tag="ACHBFW_001784"
     CDS             complement(<1..771)
                     /locus_tag="ACHBFW_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM9318088.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTK"
     gene            complement(821..2317)
                     /locus_tag="ACHBFW_001785"
     CDS             complement(821..2317)
                     /locus_tag="ACHBFW_001785"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9318089.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(2511..4133)
                     /locus_tag="ACHBFW_001786"
     CDS             complement(2511..4133)
                     /locus_tag="ACHBFW_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM9318090.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(4461..5774)
                     /locus_tag="ACHBFW_001787"
     CDS             complement(4461..5774)
                     /locus_tag="ACHBFW_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM9318091.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(6019..6243)
                     /locus_tag="ACHBFW_001788"
     CDS             complement(6019..6243)
                     /locus_tag="ACHBFW_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318092.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(6263..6685)
                     /locus_tag="ACHBFW_001789"
     CDS             complement(6263..6685)
                     /locus_tag="ACHBFW_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9318093.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(6827..7537)
                     /locus_tag="ACHBFW_001790"
     CDS             complement(6827..7537)
                     /locus_tag="ACHBFW_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFM9318094.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(7555..8052)
                     /locus_tag="ACHBFW_001791"
     CDS             complement(7555..8052)
                     /locus_tag="ACHBFW_001791"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9318095.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(8089..8541)
                     /locus_tag="ACHBFW_001792"
     CDS             complement(8089..8541)
                     /locus_tag="ACHBFW_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9318096.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(8676..9281)
                     /locus_tag="ACHBFW_001793"
     CDS             complement(8676..9281)
                     /locus_tag="ACHBFW_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM9318097.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(9731..10351)
                     /locus_tag="ACHBFW_001794"
     CDS             complement(9731..10351)
                     /locus_tag="ACHBFW_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9318098.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(10353..11222)
                     /locus_tag="ACHBFW_001795"
     CDS             complement(10353..11222)
                     /locus_tag="ACHBFW_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM9318099.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(11245..11652)
                     /locus_tag="ACHBFW_001796"
     CDS             complement(11245..11652)
                     /locus_tag="ACHBFW_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM9318100.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(11671..12960)
                     /locus_tag="ACHBFW_001797"
     CDS             complement(11671..12960)
                     /locus_tag="ACHBFW_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9318101.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT         3724 a         2757 c         2129 g         4353 t
ORIGIN      
        1 ttttgtgagg gtctctacca tcggaataaa taatgtatac ctgtttgttt tttctaaaag
       61 ttcttctctt cgaagaatgg tcaaaacgag ataccgagaa aatcgtatga agtcctctcc
      121 tgaaacaata taattatatg atataagaac ctcttttaat gtcttttgga cagtcggcat
      181 gatattggaa aaaattgtac tgatttgttg aggaaacctt gcgtgttgga tcaccgtttc
      241 tgttccaaca agagataaga aaattctttt tgaattttct tcagcaatga tttttagtcc
      301 ttttgtcgac gaaacttcca gttctaattt aggattgcgc tcgatccaac gcaaaatcgt
      361 ttttatttgt aatgatacgg ctgtttttga aagaaagaaa atgtctgcta tcttttgcat
      421 actgacatac cctttttgaa acaataagta cgtcacaata gttgaagctc ggaaatccat
      481 gctggaatag attttatgtt tcgtcacctc ttgtatgatt tgctgtttag tttcagattc
      541 aattgattct aaaaaataac ctgaacgttt atttgactgg ataatgattt tcttctcgta
      601 gatagcaagc atatttttga tttcagtttg gatcgttttc ttgttggtac ctaaagcttc
      661 agctaattct tcgcttgtta tccaatgatt cttatttctt aatactacaa gaatttggag
      721 ttgtcgttta tttaagtcca tttgcttaca cctcctcttt gttataaaca tacaggaaaa
      781 acctctcaaa tgacaatagt cacttgcaga ggtttaaaaa tcatttgaag aaagatctat
      841 attcaaggtt tttaccatta gactggataa tttgtttata atattcaaaa ctatcttttt
      901 tcaatctcct aaagcttccg ctcccatcat tttgtctatc cacataaata aagccatatc
      961 gcttcctcat ttcccctgtt cctgccgata cgatatcgat tggtccccac cataagtaac
     1021 caatgatttg gcaaccatct tttattgcct cagcaatttc ctgaagatgt atttttagat
     1081 actcacaacg ttttggatcg tggattcgat tcaattcatc tggtgcttca tctagtccaa
     1141 tgccattttc aactatgaat aatggtaatt cataacggtc tgtcaattca ttcatgacat
     1201 aacgaaaccc tagaggatca attggccaac tccaaggttc gggagatttt tccttcaaaa
     1261 agggattatc gaaaccattt gctccgccag tatctacgcg catttctgct tcttttttat
     1321 agacagcgct acgataataa ctaaatgcaa tataatctgc cgtgttagct tgcaagcact
     1381 gttcgtctcc atctaacatt actggtgcac agtcttcttc cttccatgtc ctgtaaatat
     1441 aagctggata atgccctcgg cacatgacat cggagaaaaa ccatgaattg cgtttaaatt
     1501 gcagtgaacc aaatacattc tctggatcac agtcatatgc ataagtcgct aatgcagaaa
     1561 agctacacat aatacccatc ttagcatttg gatcgatttt ctttaataat tgagtagcac
     1621 gggcgttcgc tagaaacata tgatgacagg cttgataaat cacttttttt gttagtgtag
     1681 caatgggttc aagtttatct ctagcaggga atgtaaccaa tccagcagct gcataaggca
     1741 tacgaaaagc attattgatc tcattaaagg tcatccaata tttcactttc ccttggtagc
     1801 gttcaaagac ggtctgcgca taaaattcaa aaaagtcgat taattttcga ttttgccacc
     1861 ccccatactc tcctaccaat gctaaggggg tttcataatg actaagagta attactggct
     1921 cgatatcata tcgatttaat gtattgatta gtctgtcata aaatacgaga ccagcctcat
     1981 ttggcttctg ttcgtctcct cttggaaata ttcgtgccca tgagatgctt gttcgaaaag
     2041 cttttaaacc taactccttc agtaaacgaa tatcctcttc aaatcgatga taaaaatcaa
     2101 tcgcttcatg agaaagataa tggagtgatt cgtctaaagc aatctcccaa atcttttttt
     2161 cttcattcca ctgaatacta ggatgctggt tcatgatccc gataagcgta tctgtcacat
     2221 taggtaattt gccgtcttcc aaccatgcac cttcacattg attagcagct actgctccgc
     2281 cccataaaaa attttctgga aatatttcct gatccataac tatctctcct cgctatcaac
     2341 aatttcttac ttgaagtata gctcgattca tcaaaacaag atattctaac tatttcggca
     2401 gaagagaaga aaaaaattga atgctactct tcatttttaa tatttatata taacaaatac
     2461 ataagacgat ccccgaaaga aaacactcgt taaccatcta agcaaacgat ttattttact
     2521 tccaacacaa atgcttcata aggtcgtaaa tcaactttgc cgtctggtaa ctgtttcaga
     2581 ggataatttg acacaataac ccttaaagct tcacgtggaa tagtgaacgt ctgcttttca
     2641 ctggacatat tacaaataac gagccattgc tggtcttcat atatgcgaga ataggcaaag
     2701 acatgctcag gcgtcttttc cactaattca tagtctcccc agacgatgat gtcatgtttt
     2761 ctacgcaaat cgatcaattt cttgtacata taaaaaacag aatcttgatc tgctaacgct
     2821 tcttctgcat tgatttcttt ataatttgga ttgactcgaa gccatggaat tccagtagta
     2881 aacccagcat gttcacgggc gttccattgc ataggcgtcc tagcattgtc tcttcctttc
     2941 gccttgattg attccaagat ttcctctttt gtaaatccag aagaaatcct ttcgttatac
     3001 atattgattg tctcaatatc ttttgcttcc gcgatatcat cgattgggca attcgtcatg
     3061 cctaactctt ctccttgata gatataaggg gtgcctttca tcatatggag caagatagca
     3121 aacatcttgg cactttcttt tcgatacaca tcgtcatttc cccaacgaga aacaattcgc
     3181 ggaagatcgt gattattcca aaataaacta ttccatcctt catcacctaa agatgtttgc
     3241 catttggata atactttttt cagttcagag atactcaagg gtttcaaatc ccatttgtct
     3301 ttgccttctt gctgatctaa tcctatgtgt tcgaattgga agaccatgga taattcattt
     3361 cttttaggat tggaataaag tttagcgatt tcggtagttg ctccccatgt ttcgccaaca
     3421 gtcatcagat ttttgtcacc aaaagttgct tgattcattt cctgaagata ctcatgcagt
     3481 ttaggtccat tacttgtgat ttctttatcc ggttctttgc caatcatatc gatcacgtcc
     3541 atacgaaagc ccccaatacc tttatcgatc cagaaattca tcatttgata aatctcttgg
     3601 cgaacctgtt tatttttcca attcaaatca ggttgacgct tgctatacaa gtgcagaaaa
     3661 tattgccccg ttttttcatc caactgccaa gcagaaccac tgaatattga gcgcaatcta
     3721 ttaggctctt ttccattgac cggatcacgc caaatataat agtcccgata aggattttct
     3781 ttatttttac gggcctcaat gaaccacgga tgttcatcag aagtatgatt caccaccaga
     3841 tccatgatga tacggatatt tcttttcttt gcttcttcaa tcagttcttc catttcttcc
     3901 atcgtaccga attctgccat gattgcttga taattactga tatcataccc attgtcatca
     3961 ttcggagact cgtatactgg agaaagccaa attgcatcaa tacctaaata cgccagataa
     4021 tccaacctgg aaataatccc ccgcaaatct ccaataccat cttgatttga atcttgaaaa
     4081 cttcttggat agatttgata aacaactgaa tggtgccacc atttttgttc catgattttc
     4141 ctcctataaa tggatgtttc tattttttgc ttaatcattt attttagtct ctctcactaa
     4201 aaattgtaac gctttcaaat agctattgca ccttgttatc ggtaacagaa taaaacaaaa
     4261 aaacagctga ggaaattccc tcaactgttt tttgaaagga ttgatttatg cagggaagaa
     4321 tgctgatttc acaacttctt tataaacggt gttcactcag ctgtcccttg gtattagctc
     4381 aaaaaacggc aaaatataag aaactatttt ttcgtttttt tctctaatac tcaggccaga
     4441 acgctgattt cacaacctct ttatttcaca ttcaatatcc acatgaatcc cacaaataca
     4501 aagaacagac catacatgat tggagatact tcttttcctc ttttagctgc taacatggta
     4561 attggataga agataaaacc taatgcaatc ccatctgaaa tactataagt caaaggcatg
     4621 ccgatcaaga tcaagaatga agggatcgca atctctaagt ttccccattc gatttgtcgc
     4681 aaagattgtg ccatcaatac tcctaccata atcaatgctg gtgcagttac ttgtgaagta
     4741 acaactgata gtaatggaga aaagaacaag cctaacaaga agaaaatccc tgtagtaata
     4801 gctgtgatac ctgaacgtcc tccaacggcg ataccagctg atgattctac aaatgcacca
     4861 acaggagaag taccaaataa ggaacccgca agcatagctg ttgaatcaga agctaaagct
     4921 ttccctacac gaggcatctt gttgtctttc atgaaacctg cttggtttgc taatccaact
     4981 aaagttcctg ctgtatcaaa gaaagtcaca agcaagaatg tcagcactac gacccacatt
     5041 tgaagagaat tgatgtcccc tacatgtttt aatgcaacta aaaatgttgg tttcaaactt
     5101 ggtgctgcgg caacaatttc agaaggcatt ggaatcaaac ctgtgaccag acctaaaatc
     5161 gttgtagctg ccatcccgat gaagatgcca cctggtacac gccgaacaag caagatagca
     5221 gttacgacta atccaaaaat cgtcaaccaa gtagaaccaa cgttgagtga tcctaatcct
     5281 actaatgtag attcattcga aacgatgatc ccaccttcgc tcagaccaag gaaagcgata
     5341 aatagaccga taccgccaga aatcgcatat ttcaaatctg ctgggatcgc atcaatgatc
     5401 atttcccgca gcttgaagat tgtgatcaaa ataaaaatca aagaagcaac gaatacgccg
     5461 gctaatgctg tttcccatgg aatccccatt cctaaacaaa cagaataagc aaaaaaagca
     5521 ttgatcccaa gagcaggtgc cgttccaatt ggataacggg ctaatacccc cattaaaaca
     5581 cagccgatcg cactggctaa tgcagtcgca gtaaacactg ctccttcatc cattccagaa
     5641 gcacctaata cagttggatt tacaaataag atataagcca ttgagataaa tgttgtaaat
     5701 cctgccagta cctcacgttt taccgtggtg ttcaattttt ctatttcgaa gtaggaaagt
     5761 attttttcct tcatcttcca tccccctaaa tccgaacatt ataaataaag tccgttaaaa
     5821 tttcgattta ttaataaaac aacgtgtttt atcatatact aaaacaaaac ataagtaaac
     5881 aaaaaattcg gattttaagc aaaatccgaa cgttccatct gattaaatcc ctttatctca
     5941 aagttcatga acattcactt tggattacaa gaatttcttt attgtgttaa taaagtttct
     6001 tcattctagt ctcaaaaatc aatagtgtga tttgatttta ccatttcgtt tctgttcttc
     6061 tttgatgaat tcttttgtat cgacaggatt cagattattt agtccgccaa aacctgtatc
     6121 gaccattttt ccccagcctt ttgtcttctt ttttcctgat ttgagattct ttgctcgaag
     6181 cttctcacta tgttttgctc gccgatactt caaataacct tctaatcttc ccattagatc
     6241 cactgttttt cctccttttt attcaatcag acgactcagc ttttcctata acaaaataat
     6301 tgccttcagg gtcagaaaaa ttgaaagtca agatgccatt gttatctgtg atttctcctg
     6361 gttcttccat gttctcgtag atagtttcaa attcatccgt aaaaaatact aaagaaggcg
     6421 gtggtcctaa tacctctggt gaaaacttct gcacaaatac ttttgggaat acgcctaatt
     6481 ctaattcttt tgagatgctg agtacaatac tcttaaattc ttctggcaat tcgattgttt
     6541 cagaaatttc tgcaccaatt ttttctacaa agaatcggca aatcatctct acgtcattga
     6601 cataaagcat cacacgtgtt ttttgtatca tcatttgacc cttctttcta tttgattttc
     6661 tttcctgtct ataaactgat tccattatat cacgaaagcg caaacacctt gtgtttgata
     6721 ctattcaaga aaaataaagc aatagtttct aggattgctt tcctccacaa acttttttta
     6781 caaaaaaaaa acggcaatga atgccgctct tcttatttcg attcttttat aattctgctt
     6841 ctgattcatc tttggttagt gtgtgtctaa cactagttac gccaaatgtt tgatcgattt
     6901 tttccttcaa cgcttcgatt gcctgtgcgc cacgtgccgc ttgttcagca ttgatggatt
     6961 caatgaccag caccgctgct tgtgtttctt ccgtcagcat cgttctttgg gcttcacgcc
     7021 agttgagaca gcgacagatc gctccttgtt catcatagta gatgatttct tccggaagtg
     7081 ccggagcatc ggaatctgca cctaaaggca ggaatgattc gccaccttgg gcttttccta
     7141 aatgcagatt tcctgcgatc ttttcgatat cttctccgcc gcaaggtacg ccaaaagaca
     7201 aggaaatact attatagata tctacaagcg gattgattgg gaaaaattcc cgttcttgat
     7261 ttgcacgttt cagtaaagct tcaatagaag aacgtgcacc ttttttagtc ttgaatgtgg
     7321 taaatgcctg acgccactga gcaatgactt cgttttggct gaatgtttct tctgtaaggt
     7381 aatttcttgc ttcttttgct gcctcattca ataattgatg atacgtttct tcactttctt
     7441 ttgttacgtg gttatcgata ccatttacgg ataagatttc gatggttgct tcaggaaata
     7501 cttcccagaa attggtatcc actattactt ttttcatctg ttcacacctc ttgattagaa
     7561 taattgcttg atgaatagat agtctgtttg gggatcatct cccatataaa aaacatgttc
     7621 gctgtatttg ataaatcctt ttttacgata aaaagcgagc gcaacatcgt tgtgttccca
     7681 aacaccgagc caaatggctg ttttttgctt ttcttttgct ttttcaatag ctgtatctag
     7741 taaaaatgta ccgatacctt gatgtttaaa tttttttcgt acatagattc gctcgatttc
     7801 taaaaaatga tcagccattt cctcagattg actcgttcct gtatttagtt tcaggtaacc
     7861 cgcaggctct tgatcgatac tcactaggaa aaaatcggtt tctttatctt ttagctcttc
     7921 tgtaagtttt tctagtgcat aagcttgtgt caagtaatca gctaaatgct ccggtgaatt
     7981 atcttttcca aatgtatctg taaatgtttc gatactaatt tgttgtagta agtctacatc
     8041 tgaaggcgtc atttttcgaa tttcgtattt attcacggct tcttcctttc aatagatcct
     8101 tttattgcct tttttgacaa aattccaatc ttcgctaaca ttcttgcata ctttatttaa
     8161 aagatattcc aattgttttg cttcttcgtc agaaagacca tttaatgcga ctgcattgga
     8221 gtagttgttt tctcgaatga tgaaaggata gatttctgct ccttttttag tcggatagag
     8281 tttcttgatt tttttatttt ctttatcttc caaacgttcg atcatcccat tggattctaa
     8341 tttcttgatt gctcgggcag tcgttgtacg atctactttg atcatttctg ctagtttttc
     8401 ttggatgatt ccaggatttt cgcaaatacg tactaaataa agatactgtc ctcttgttaa
     8461 atcgacttct ttgaattcga tattgctaat agaatctagc gctcgggcaa tcattccgat
     8521 gtctcgaagg atttcagcca tttcatccca cctttatttt gtttgtaaga ctagttcacc
     8581 tctttttgtt gcaaatgcaa ctattttcaa taaaaaaatc aagttctttt ttaataacta
     8641 gtaaagtata ttcttttctt cttttacgaa aaagctcaat cacaatagat ctggtttttg
     8701 ttttctaaaa taagtaactg tttcttcgtt gttcgctttt ctgatagctt tggcaactct
     8761 tctatactaa aaaatcccat gttggaagtt tcattattct caataaactt ggcctcttca
     8821 ccgtggatcg cacaagcaaa aacaagttta taatactgaa aaagctgtgg aatatctttt
     8881 tgtttattcg tatcaaaaac tgctcgcaac tgagtagttt ctactgttaa tcctgtttct
     8941 tcatagactt ctttacgcac attttcttca ggagacaatc ctatttcagc aaatccacca
     9001 ggaagtgacc attctttcgt ccgtttatct tcgattagta ggaccttgcc atcttttttg
     9061 atataggcac gaacatctac ttttggtgtt ggatgtcctt ctccaatcgt tgtgagcgct
     9121 tttatttctt cgaaagtttc ttttcctatt tcactgatca gttccaatga aatcgtccgg
     9181 atctcctcaa aacgctcttt atcaaataca tcttttccat aatatagacc agcttgagaa
     9241 agtgcgagta gtcttttgta ttgagccaac ttcttgttca ttctcttccc ttctttttta
     9301 gcaaatgtat gactatgtaa tcaagtatag aagactggtt taattacggc aaataaatat
     9361 aatgaaatct gcaataaata aaagaatgtt aaaaaactcg agaacaaaag acattcgact
     9421 tctctggggc tgtgaataaa tggcgttcaa aagcttgcat ctgcggtaat aagccccacc
     9481 aaacgaaaaa tatgacgagc tattcttgtc tggctgttct tattaattga ggcaggacag
     9541 ctttttcaca acctccccac taaacggtgt tctcctacca actcctcggt cacaagtcac
     9601 aatattcatc aaacatgaga agctgttttt ttgaaattgc tccttgtgac tcggagctga
     9661 acgacaggct cacgacctct cggtaaacag cgttcaaaag ccaatttttc gatattaagc
     9721 catttctttg ttacttattg tcattgattt ctttttctaa tttatccaat agtggagtgt
     9781 aatccacttg agtatagtct gttacatcaa attcatatag tgttcctaac tggaatttgt
     9841 catattttcg gtcggcgatg atctttttga atgcttcttt cgtaatatga ttggtccctt
     9901 tcgttcgatc ttccaatacg tcaccatagt gataatgatc catgatatag ctcaaatgac
     9961 gatctgattc acgatcacgt ttgtaccgac gttgccataa cacttcaaag tctgctgtta
    10021 ggcgaatcgt gatcacctga tatccgtatc tatctgctaa ttcttggagt cgtgcttttt
    10081 gcttatcact aaaaggatac tcggacacaa tcactttctt tcctgcttcc atatacaaat
    10141 caagtgcttt gtaatagaaa ggataaactt tcgtcctttc ttgttccgcc cgttcttcca
    10201 aattgttaaa cccgatggat tctgcatagt attccttgat ttcatcgagc gtgatggcat
    10261 acatatctgg aaatcgttcc cgtagtttat tcataagata tgtttttcct gtaccaggac
    10321 taccagccag aagaatgaaa tactttttca tctcatttat ttcctaagtt aattggactt
    10381 tccaagttca taaacagtcc aatcttatgc ttgacaattt cttttactgc ttcatttgct
    10441 ggaatcacat tatggcgaag agacttgttg atttctgttt cactaaaggt ttctttagct
    10501 actttcgtcc agttgactaa aagctccgtt ccaacattga atttgcggat cccgttattg
    10561 atcaattcag gataatcttc ttctttcaca cctgttcctc catggataac tagggggaca
    10621 tcaactactg catcgatttc tttcagcaag ggaatgttta cttctgtttt tgatttgaat
    10681 tgtccatgat tcgtaccgat agcaatagct aatgcatcca cgcctgtagc ttctacaaat
    10741 ttcactgcgt cttccggtct tgtatatacc ttgtcatttt cagcgacatg gattccttct
    10801 tctgtaccgc cgattgttcc aagttctcct tctacggaaa caccgtgcgc atgggcatat
    10861 tctactactg cttttgtctt caggatattt tctttgaaag gcagatggga cccatcatac
    10921 atgacagaag tatatccgct gtcgattgct tctttgatat catcaaagtc tcgggcgtgg
    10981 tctaagtgca agactgcatc aaccatttct tcttctgcaa tagctgtcat tacattgact
    11041 aacacatgca tgccgatata tttagcggta tcgacacttg tttgaataat gatcggcgaa
    11101 cccatttctt ttgctgcgcc tagcatagca ggaagcattt ctaggttatg cgtattaaaa
    11161 gctccaactg taaaattcaa ctcttctgct gtttttgtta cttctttcaa tgttgtatac
    11221 atcttttatt gccaccttat ctttttattt gtttcctttg atcgttactt gttttgcaat
    11281 ttgtcgcatc tcatccattt tgcccatgta gtaatcgatc ccttgatcat caccagcacg
    11341 agcagcatct gcacgcttct ggttatagag gctcatcagt tttttatacc catctccgat
    11401 cgtctgttta ttttccaaac tcttttctaa atattggatg gcttgatcgt tcacatacaa
    11461 ttcagaaaat ttaagtccaa cttgttcata aagtttcgct aatttatgct tatcttgggt
    11521 agtggaaatc tcttgtttca attgtgtaat ctctgattga atcttttcgg tcgcttctgg
    11581 cgatagggct ttttcttctt ttgccagaac ttttttcggt tctttttctt ttttcttaaa
    11641 aaatccaaac atcattttcc ctactttcaa ttaaaatgct ttcaaaactg ggcttaagat
    11701 ccaggcatat aagagtgctg cggctactgt atctacatcg gttgctgtag cattgttgaa
    11761 tcccatcgaa ttaaagatcg ttactagcaa agcgggcaat aatgtaataa agaaaccatg
    11821 agcaataccg ccaatgatcg ctcctcggcg tccgccaact gcgttgccaa atatccctgc
    11881 tgtccctccg gcaaagaaat tggttagcat accaggtaaa atcatcgcta agccgaacat
    11941 gggcaaagta aacatggcaa taatcgtacc aatcgttgtc gtaatgaaac ctaaaatgac
    12001 agcatttggg ctataaggaa agaaaactgg acaatctaag gcagggatcg catcaggaac
    12061 taatttcata gctatccctc gaaatgccgg cacgatttca cctaacagta gacgtacacc
    12121 agctagcaat acatacacac ccactacgaa ttggatggct tgtaaaaagg caaacatgat
    12181 atagttttga tctcctgata attcagaagc gaatggttcg cctgcaaata aaacagtgat
    12241 gatatatagc gggaccatca cgaccatgac agacaaataa gtatcttgca gaaattcgaa
    12301 atgtgtcggc aacttgatat cttcgatcga ctttttattt tcgcctttct caccaaaaag
    12361 cttagcaaca ccggcttcaa ataagtaccc gatcgtacaa aaatgaccta aagcaatatc
    12421 atttgatcca gtgattttac gaatgatcgg ttgcgctact gccggcatag caatggcgaa
    12481 acaagctccg ataaaaccac cgaccaagat caatacgatc cctcgcagac cggcaaaata
    12541 gccaaagact gtcgtcatcg ttgccatcca taaaattgct tgaccagtta aaaaaatgta
    12601 cttccatttt gtgaaacgag ccaaaataat attgaataca aaaatggcta agaacgtcag
    12661 tgcaatatct ctacctaaac ccaaatcatt cattgcttga ccattgatag attcaataga
    12721 agggatgatc ccttgcatat gaaatccttc agtaaagatt ttcccgaaat aagttaaact
    12781 tccaacgatg atgctcgaac cggcactcaa cacttgaaat cctaggagtg tctttaacgt
    12841 gccagagatg acttgtcctg ctgatttctt ctgcagtagt agacccaaca tagcaatcaa
    12901 tgcaatggtg atcgatgcct gagtcaaaat attttcgatg ataaaattga taacgcccat
    12961 gtt
//
LOCUS       DAYYME010000074        12835 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000074 DAYYME010000000
VERSION     DAYYME010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.075"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..3579
                     /locus_tag="ACHBFW_001798"
     CDS             52..3579
                     /locus_tag="ACHBFW_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM9318102.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3576..7298
                     /gene="addA"
                     /locus_tag="ACHBFW_001799"
     CDS             3576..7298
                     /gene="addA"
                     /locus_tag="ACHBFW_001799"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFM9318103.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7367..7708
                     /locus_tag="ACHBFW_001800"
     CDS             7367..7708
                     /locus_tag="ACHBFW_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM9318104.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8017..9060
                     /gene="pheS"
                     /locus_tag="ACHBFW_001801"
     CDS             8017..9060
                     /gene="pheS"
                     /locus_tag="ACHBFW_001801"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFM9318105.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9065..11485
                     /gene="pheT"
                     /locus_tag="ACHBFW_001802"
     CDS             9065..11485
                     /gene="pheT"
                     /locus_tag="ACHBFW_001802"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFM9318106.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11655..12665)
                     /gene="trpS"
                     /locus_tag="ACHBFW_001803"
     CDS             complement(11655..12665)
                     /gene="trpS"
                     /locus_tag="ACHBFW_001803"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFM9318107.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4350 a         2237 c         2727 g         3521 t
ORIGIN      
        1 ttttttgata taataaagga gcaccgtatc atgtgttgaa ggaggaaaag catgtcttta
       61 caatttattc ttggaaacgg gaaaaaagat caccgtcaag tcttgatcga cgaagcatcc
      121 gcttggctcg accaggatgc aagtaaccaa gtatttttcc ttgttcccaa ctacagcaaa
      181 ttcgaacagg aacaagaaat tttagcggag atgcgaagaa gaagcggaaa aaaaactttc
      241 agcacaacaa acgttcaagt atttagtttt tatcgtctgg cttggtattt gctgcaacag
      301 acaactcttc ttacaggaaa tgaattatca gaatcaggat ctgcaatgat cttgcgacag
      361 attttagaaa aaaatgagga agaattaaca atctttcgcg gagaaatcaa taaaagaggg
      421 tttattcgtc aattacaaga actctacagt gaattgcgta caggaaatat tcaaccagaa
      481 gacctgatgc ttttatttgg tgattcacct aatgcagaag accagcaact taaaatgaaa
      541 gacttgaagc tgattttttc agcttatgac agcgaattga ctcaacgtgc actaacaaat
      601 gaagatgcac tatctatact agcaaattat atgcagacac aagatttcaa caacgtcaaa
      661 tttattgtca gtggtttttc tcgaatcaat ggtcaagagt atcaattact gcaggcaatg
      721 atggaaaagg ggcaattggt tgtcgattta ttgctggatc gtccttatac agcggatctt
      781 ccagaagtac ttacgctctt tcatgagact ggaaagcttt atttccgtct ttttcaaagt
      841 gcaagagaac ggaaagtccc tatattagtg gaccgatttg caccagagca gactcgtccg
      901 acagagcttc aactgctgca aacaatgtgg gaagagacat ctagccaaaa gaaagcggca
      961 gtacacaaaa tagggggaca agagcatctc catttgtggg aagctgaaaa tccaactgaa
     1021 gaagtccgac aattagctgt tgaaatcaga cgactagtca gtgaagaaaa ctaccgttat
     1081 cgtgatatcc aagtgctgac aaaaaatatg tctttatatg gaaatatcct tcggccgatc
     1141 tttcaagaaa tggcaattcc tttttatatc gatgaagaac aaatgatgga aaatcatccg
     1201 ctgatcgaat ggatcaacag tctgttcgct cttgaccgct attcttatcg gctaagtgat
     1261 attatgcgtt ttttcaaaac tgaattattt ctgtcgacgg aagcagaaac cgaacttgat
     1321 gaatggaaac aagagcgaga tgaattcagg aaaaaactgg acatcactga aaatgtagca
     1381 cttgcttaca attatcaagg aacttattgg actcgtgaaa aagactggca gtttgtggcg
     1441 tatgattttg aagcagaaca gattgaagac gtacagtatt tggaagaaca gtcaaatacg
     1501 attagacgat tatttcagaa agctgtacca gtatttttga aaaaaataaa gaaaagcaaa
     1561 acaggacaag aggccgcaaa tgttttttat caatttttga tccaaagtgg cgtacagcaa
     1621 caattattgt tttggcgtaa tcaggaagta gacagaggaa acttagaagc ggcaagaaac
     1681 catgagcaga catggggagc gctgattgat ctgttggatg aatatgtagt aatttatggt
     1741 gaatcggctt ttgactggtc actgttccaa gaaatcatta caagtggtct agaaaatcta
     1801 agttatggta aaatccctac cgcaatcgac caagtgcgaa tcaatcgttt ggaattagtt
     1861 cgaccaaatc aagctgccgt cacttttgca ttagggttga atgatcaagt gtttccagac
     1921 cgaaaagaga caaaaggatt gttgtccagt gaagaacgag aagtattaaa tcaggccttg
     1981 ccagatggac agtttttatt cgacccatca aaggaaagta tctcttttga gcctttccaa
     2041 gcctatcttg tttttctttc cggaacagat cgattatatc ttagttatgc acaaagctat
     2101 gacacagatg gttcgctgaa gatgtctcct tatttgcgta gaatcacaga atatttatct
     2161 gttcccgtcg agagaaaaga gcatttgacg atcgaaagcg accctaattg ttatgtaggg
     2221 acttacagaa gcggaatcaa tacagtcaat aggatttatc gattagcttt agatgaaaaa
     2281 aaacctgtac caaagcactg gcaaaagctc aaagaattag tactgaattc gtcatggcga
     2341 aattttgctt tacagatttt tgaaagtcag gaacatttaa atgttcctgt ttcgatgcca
     2401 gaaaaaatgg cggaagagct ttatggaaaa gagatttata cttcaatttc acgtatggag
     2461 aatttttata attgtgaata caaatacttc atgcaatttg gtttgaaatt gaaagaacga
     2521 actgtttatg gtctaactcc agcagcaact ggtgattttt atcatgaatc attggatcgt
     2581 ttcttcaagc tactgttttc aaaccaatta tccttagtga atatgtccga tgcgcagcgt
     2641 agagacttta ctgaaaaagt gctgcaggaa gtattcggag aacttcggtt tgagatattg
     2701 gatagttctg caagaatgaa ctatatccgc taccagttag gacagacgat tcaaaaagtg
     2761 acatgggctt tgcaaaaaca gagcaaaaat acaggcatgc atcctcttca gacagagatt
     2821 ctatttggtc agattgctgg agcaaaaggt atcacaggtc tggaattacc gttgagtaat
     2881 ggtggaaaac tccatgttcg agggaaaatc gatcgaatcg atgtagcttc tgaacaagaa
     2941 gatacttggc taagcgtcgt tgattacaaa tctagcggac gttcattcga tgtcacagaa
     3001 gcttattatg gcatggctat gcaattattg acgtatctgg acgtagcatt gatggatgcg
     3061 gtacgtttga ctggaaaggc ggctgttaaa ccagcaggat catattattt acatgttcat
     3121 aatcctgtat tgacggagac agatgatgta gagaagaata cattgaaaaa atttagttat
     3181 gatggcgttt ttgtcgatga tcccgctctt ttagaagtac tagatcattc actggaagca
     3241 aaagaacaat cactgatcta tccagtgaaa aaaaatgcga aagatgaata ccaaaaaggt
     3301 agtttcagta aagacaaatt tttcacggaa gaagaactgc aactattcat ggcgcataat
     3361 cgggaaaata tgcggttagc tggagatgcg attacttccg gagaaatcaa actaaatcca
     3421 gcttataaag ataaacagcg gattgcctgc gaattttgtc cattcagaag catctgtacg
     3481 tttgatgtga tgctgaaaga aaataattac caccgtctgg agaaaataga taaggaagaa
     3541 gcactgcgta gaatattgaa aaggagtgaa gaagagtgaa tcaaattcct ttaaaaccgc
     3601 aaaatgaacg attcacagat gatcagtggc aagcgatttt tgatcaagga gataatctat
     3661 tagtctctgc gtctgccgga tcaggtaaaa ctacagtgct tgttcgaaga gtgattgaaa
     3721 aactaaaaat gggttttgat atcgatgaat tattgattgt gacatttact gaagctgccg
     3781 caagagaaat gaaagaaaga attcaagagg cattacaaga atccgtcaat agcgaaagtg
     3841 accctgtacg tagacagcat tttactaagc agttagtttt attgcccaca gcgaatatct
     3901 caaccttaca tgctttttgc ctgacagtta ttcgacgtta ttattactta attgatattg
     3961 atccagtctt tcgcatgtta acagatgaaa cagaaacgat cttgatgaag gaggatgtgt
     4021 gggacgagct gagagaagca ctttatgcag aaaatgatga acgatttttc caattaacaa
     4081 tgaatttttc taatgatcgt tcagatgacg gcttgacgaa tctggtgttt tctctttatg
     4141 agtttgcccg tgccaaccct gacccccaaa aatggctaga acagttaagc gataattatc
     4201 gtttgcctga ggggctagcg aaatctcgat tatatcaaaa acaaatccgt cctttagttt
     4261 tggcagatat ataccaatgt gtacagttat atgaacagat gactcagtta gcacaaggtg
     4321 aaggattaga aaaaatgcat gagcaagtag ctggagaaca acagcagatc aagaatatct
     4381 atgaagcgtt ctcgcaagat cggttagaag aagcttatgc tggtttggaa caactaactt
     4441 tttcaacttt taagagcagt cggaaagcag aattgaaaga aatttccaat gaagtcaagg
     4501 gcatgaggga taaagcaaaa aaactgattc agcagataag taaaagttat tttcctgttt
     4561 ccccaagcca aatggaagaa ttaacggaca aagcactacc gttagtcgaa gaaatgacaa
     4621 aagtcacgca aagcttcatg gacggtttca gtatgcgtaa acgggaaaaa ggtgtgctgg
     4681 atttcaatga tctggaacat ttagcgttgc agattctcac agaaaaaacg aaagatgtat
     4741 ggctgccaag tgaagcttca aaacattatc gaaaaaaatt caaagaagta atggtagatg
     4801 aatatcaaga cgtgaaccaa ttacaagaag ccattttata ctggctaaga gaacccgatg
     4861 atacaaaagg aaatatgttc atggttgggg atgtcaaaca atccatctat tctttccgtt
     4921 tagcagatcc tagtttattt atcgggaaat acgagaattt ctcaaagaaa gaaggcggac
     4981 gtcgaatcgt tttggctgag aacttccgct ctagaaaaga agtcctttct tttacgaatc
     5041 ttatttttga acaactaatg gatccagcag tgggtcaaat caattatgac gaagcagcca
     5101 aactgatcca aggattttca gactttccag agaatgaaca gtttgagccg gaaatcatga
     5161 tctatgaaaa agaacaggaa gaatcagaga ttgagattcc gacggatgat atactggaag
     5221 acaaaacaga aggcgaactt tttatgacag gactaaaaat tcgtcaatta attgattcct
     5281 cctttatgat ttatgataaa aaatcaaaaa aaagtcgtcc gatagagtac aaagacattg
     5341 ttctcttgac acctacaaag aagaacaact taacgatttt agagattttc aaaacattgg
     5401 atattccatt agagatgaat gatgctcaaa actattttca agcgacagaa attcgcacga
     5461 tgatctcgct gctacaatta atcgataatc cctatcaaga tattccgctt gcggctgtat
     5521 tgcgctcacc gatcgtgggt ctgattgaac cagaattagc cagcattcga ttagctaacc
     5581 gtgcacatac ttactatgat gctgtgcttg cttatcaagc atcaaacgaa gatgaattag
     5641 cagctaagct ggaacatttt ggcaagcaac tagagcattg gcgagaactt gctcgccgtt
     5701 cttcgatcac tgacttgtta tgggatatct attatgagac gggatatcta gagtatgtgg
     5761 tcggtctgcc agcaggggca cagagacaag ccaatcttta tgcattggtt gatcgtgcca
     5821 aagcttacga gcaaagttct ttcagaggat tataccagtt cgtgcgtttc attgaaaaaa
     5881 tgcaagaaaa agataaagac ttagcagaac cagtcatttc aatagaagat aatgctgttc
     5941 gcgttatgac gatccatgca agtaaaggct tggaattccc agttgtattt ttgttagata
     6001 tgacaaagga gttcaactta caagacttgc gcaatcggta tgcttttgaa gaaaaacttg
     6061 gagcaggaat tcgctatatg gatcctgaaa cacgagtgtt atatgatacg ttaccgtttc
     6121 aagccatcaa attggcaaag caaaacaaat tgttgtcgga agaaatgcgt aaattgtatg
     6181 taggattgac tcgggcagaa caaaagcttt tcattgtcgg ttcctataaa aataaagagc
     6241 aaatgatcca aacgtggtct gaagcagctg atcatgagga gttagttttt gatcctgcgc
     6301 tacgtttgaa gggcagaagc agcttgatga attggattgg ttacggattg atccgtcatc
     6361 ctgaaatgca gaaatactta gaggaagaaa tttcgacttc tcttcttcaa cattcaaatg
     6421 cacagttttc tatttcatgg atgaatcagc aatcgatcat tgaacaaaga cagttgcttg
     6481 ctgaaaaaga actggttaac ttagatcagc agatgaaaga agatgaaact ttattagcag
     6541 attcgttaca aaaacgtctg gcatatgaat atccttatca agcttcttca caaaccacaa
     6601 gttatcaatc tgtatctgaa atcaaacgat tattcgaaga tccagatgat acacaagaat
     6661 cacgtttgac tctggaaagc agtcagaaca aagcagcatc ccgtcaattt cgttatacgc
     6721 aagaacaact agcagaacca aaatttttgc aaaaagatcg gcaagtgtca gcagctgctg
     6781 ttggaactgc tacgcatgct ttgttacaat tactgccatt agagatgcca actacagaga
     6841 gtatccatca aaaattacag gaattggtaa ggaaacggtt agtagacgaa aaagtcgcga
     6901 aaaaagtaga tgtttcttct attatatggt tctttcaaac agaacttggt caacagctga
     6961 ttgccaacaa agaaaacgta aaacgagaac aacctttttc catgctgctt cctgctgacg
     7021 aagtatttca agactatcca aatcaagaag atgaactatt aatccacgga atcgtcgatg
     7081 gatacctaga agaaaaagat catctcaata tctatgattt taaaacagat tttattttac
     7141 atccggatga tccagcagaa atagatgcaa tcgtccaaaa atatcaagga cagctaagac
     7201 tttaccaaca agcaatgtca gaagctttga ataagccagt agaaaatgtt ttcctaattt
     7261 tgcttcgagt gaaacaaatt ataaacatta acaaataatt ataattgagc aaacttgact
     7321 tatgttttgt aaggattatg ctatactctg ggaagaggtg agtatgatgg agctaacaca
     7381 gacagaattc gttttatgcc ctaaatttga aaaaacattc tctattttag gaaaaaaatg
     7441 gaacggattg attatcgatg tactactcga aaacggtccg caacgctttg ttgagcttgc
     7501 ggcaaaaatc ccgatggtca gtgatcgagt attagtcgaa cgcttgaagg aattagaaaa
     7561 agaaggaata gtcactcgaa actttgcctg tagcgaaaag agccgttcag aatatctctt
     7621 gactgaaaaa ggagcagact tgcaaaaagc gatgcgtcaa atacaagttt ggagcgaaaa
     7681 atggatcaca gaggaagaat gcggttgatt ttgtaaaaaa aatcttgtaa actatagtcc
     7741 atacgaataa gtaaagaacg atgacgaaaa agagtagttt ggctaaacca gttcagggag
     7801 atctgtcgca gactgaaagc agatcctgtg tttgaaaaat gaagttcact ttccgagttc
     7861 gtctgataaa atcagaccgg caattgtcgt taacgaagta agagtgcggt gtaagtcatt
     7921 tatccgcaaa ttaggatggt aacgcgaaga cctcgttcct taacaggaat gaggtctttt
     7981 tgttttgtat ttggaattta aaggaggaac aagcacatgt ctctacaagc acaattagag
     8041 agcttgaaag aagcaacgct cgtcaaaatc acagcggcac aagatttaaa tgaattaaat
     8101 caaatacgtg ttgaaacgtt aggaaaaaaa ggaccgatca cagaagtatt acgtgggatg
     8161 aaagatctaa gtccagaaga gcgaccaaaa gtcggaagtt tcgcaaatga gatccgagat
     8221 cttttgacag aagagatcga gcgaaaaaaa caagtattag aagaagaagc gttaaatgca
     8281 gctttggcaa aagaaacaat agacgtgaca ctacctggta gaaaagtaaa acatggtaca
     8341 cgtcatatcg tgacacaaat catggaagaa attgaagata tttttgtggg tatgggctat
     8401 cagatcgtag aaggatatga agtagaatct gatcattaca atttcgaacg gatgaatctg
     8461 ccaaaagatc atccagcaag ggacatgcaa gatactttct atatttcaga cgagatattg
     8521 attcggacac atacttcacc agtccaagca cggacaatgg aaaaacatga tttctctaag
     8581 ggtgctctac gaatgatctc gccagggaaa gtcttccgca gagatacaga tgatgcgacc
     8641 cacagtcatc agttccatca aattgaaggg ctagttgttg ataaaaacat cacaatgggc
     8701 gaccttaaag gaacattaga agtcgtaatg aaaaaaatgt ttggggaaga tcgtgaaatt
     8761 cgtttgcgtc caagttattt cccatttacg gaaccatcag tagaagtaga tgtcagctgt
     8821 ttcaaatgtg gtggtgccgg ttgtaacgta tgtaaataca ctggatggat cgaaatttta
     8881 ggagctggca tggtacaccc gaatgtgttg aagatgtcag gaatcgatcc agaagaatat
     8941 tcaggctttg ctttcggcct tggaccagac cgcgtagcta tgttgcggta tggagtgaat
     9001 gatatccgta atttctatca aaatgactta cgtttcttaa gtcagttcaa gggggagtaa
     9061 gaaaatgtta gtttcatata aatggctcaa tcgttatgta gatttatcga atgtaacacc
     9121 aaaagaatta gcggataaga tgtctgtgac aggaatcgaa gtagagggag taacggttcc
     9181 agaagaaggc ttgaaaaaaa tcgttgtcgg tgaagtcaag gaatgcgtac cacatccaga
     9241 ttctgatcac ctttctattt gtcaagtcga tattggcgaa gacgaactgt ctcaaatcgt
     9301 ttgtggagcg cctaatgtga aggttggaat caaagtgatt gttgcacttc ctaactcacg
     9361 tatcgctggt aatgtaaaaa tcaaaaaagg aaaaatgcgt ggccaagtat caaatggcat
     9421 gatttgctca ttacaagaaa ttggctattc agatagtgtt ataccgaaag aatacgctga
     9481 aggtatctat tatatgccac aagaagctgt aaatggcgat ccagtttttc cttatttgga
     9541 tatggatgat tcgattattg aattatctat cacaccgaat cgtgcggatg cattgtcaat
     9601 gcgtggcgta gcttatgaag ttggggcaat ttatcgtcag actcctacat tcgatgacga
     9661 aaagctggta gaatcctcac gtcctgctag cgataaaatt caagtggaag ttgaagataa
     9721 agaagcagtt cctgcttatc aaatcagaat cattgaagga gtaagaatcc agccaagtcc
     9781 tcaatggctg caaaatcttt tgatgaacga aggcattcgt ccaatcaaca atgtagtaga
     9841 tgtgacgaac tatattttgc ttttattcgg acaaccacta catgcatttg actacgacaa
     9901 attgaaatca aaagagatct ttgtccgccg tgcaaaagaa aatgaaacat tagtaacact
     9961 agatggagaa tctcgggaat tgacaactga caatctcgtc attactaacg gcaaagaacc
    10021 agttgcttta gctggtgtca tgggagggtt agactcagag atcacgaatg aaacaacaac
    10081 agttgcacta gaatctgctt tatttgatcc aatttcgatt cgtcgtacag caaaacaatt
    10141 taatttgcgt agtgaatcct ctgcacgttt tgaaaaagga atcaaccatg cgactgttgg
    10201 agaagccagt gaggttgctg cagcaatgat cgtttctctt gcgggtgggg aagttctaca
    10261 aggcgcggtc aaaggctcag agatcaaagc agaagacgta tcagtgaaga tttcacttga
    10321 acgcatcaat caatatttag gaacatctct tgtcgttaaa gaagtaaatg agatttttga
    10381 agcacttgga tttagctatg aagaaaagga gtatgtttat tccgtagtag taccaccaag
    10441 acgctgggat atccaaatcg aagcagatat tatcgaagaa gttgctcgta tttatggata
    10501 cgaccagttg ccatctacac ttccaagtgg agaaacggtt gctggaagct tgtcaaaaga
    10561 acaagaaact actcgtaaaa ttcgtacaat ctttgaagga agcggattga gcgaagcaat
    10621 cagttatgca ttgacaacag aagaaaaatc aagacagttt gttataaatg aaagtaatat
    10681 tacacgtttg gactggccaa tgagcgaaga acgttctgta ttacgaatga atttgatcag
    10741 cggactatta gatgatatca gttataacac tgcccgaaaa aatacagaga ttgctttata
    10801 tgaaattggt cgggtgttct tccaagaaaa tgatccacgt acacatctgc cgcaagaagt
    10861 aaaacatgca gcaatcgcta tttctggtat ttgggaagaa aaagattggc agacgaaagc
    10921 tcaaaatgta gactttttca ccatcaaggg tcttttagaa aacttatttg aacaactagg
    10981 catcactgaa gatgtccatt atcaaacagt gacagaaatg aaggaaatgc atcctggaag
    11041 aactgctgcg atttaccttg gcgaaaaatt tattggtttt gtcggtcaag tgcatccaac
    11101 gattacaaaa agttatgata ttccagaaac atatgttgct gagtttgatt tatctgctat
    11161 agtggaagct gctcaaaaag ggatcgtttt cgaagcagtg acaaaattcc cagcagtcag
    11221 ccgggacatc gctttgcttg taaaagaaac tgtgacaaat caggaattaa cagaagttat
    11281 ccgttctgct gccggaagat ttttaacaga tatccaatta tttgatgtct atcaaggaga
    11341 aaacattgaa aaaggacata aatccatggc gtatagcctg acattcgtca acccagaagc
    11401 aacattgaca gatgaagaaa tcaataaagc aatggaaaaa gtcgaaaaag cgttggtaga
    11461 aaacctagag gcaagtattc gctaagaagg aaaatatagt aagtaacgat caagtagctt
    11521 ttgaagacca tacatttgaa aaaaagatgt gacacaactt cgtcacatct ctttttttga
    11581 ttttaaaaaa ggaatagcac agagacgaga taaaaaaaca ggcctttaca gcctgctttt
    11641 ttagagtaaa tgaattaatg gaagtaattg attcccattg ctgattttac ttcagaaagt
    11701 gtttgtgcag ctactttagc agcttcttca ctacctttac gtaagatttc cataacagct
    11761 gaaggatctt tttccaattc ttctcggcgc gcacggatcg gtgcaaattc agcttccaat
    11821 acatcaatga ggtatcgttt gatctttaca tcaccaagtc cgccgcggcg gtaatgagct
    11881 ttcatttctt caattgcttc tttatctgtt ccgaatacat ccaagtaggt gaagaccata
    11941 ttcccttcta cttgtccagg atcttgtaca tggatgtggt ttggatcagt atacatactc
    12001 atgacttttt tctgtaagac atcggcagga tcggagatat aaataccgtt tcctaacgat
    12061 ttactcattt tgccatttcc atcgattcca ggtaatctac ccattccttt tggtgggaat
    12121 acgccttttg gttccaccaa gacttcacca tatgtatgat tgaagctttg tacgatttcc
    12181 tgtgtctgct ctagcattgg tttttgatcc tcaccaacag gtacaagatt cgctttgaat
    12241 gcagtgatat ctgctgcttg agaaacagga tagataaaga atcctgtagg cacgctttca
    12301 ccgaattttt tttgttcgat ttctgttttg acagtcggat tgcgccgaac gcgtcctacg
    12361 ctgactaagt tcaaataata catggttaat tcagctaatt ccggaatttg tgattggata
    12421 aataaagtag attttgcagg atctaatccc acggctaaat agtctaaagc aacttgcaag
    12481 acatttgatg aaaccttctc aggattttta gcattatctg tcagtgcttg catgtcagca
    12541 atcatgacaa acagttggtt gtcttcatct gcctgcatag ccactcgggt ttttagagaa
    12601 ccgacgtaat gccctaaatg cagcttccca gttggtcgat ctcccgttaa aataatattt
    12661 ttcaaaaaag acacctcttt acgatttgta agtctgagca cttaatatct taccgttttt
    12721 cctgaaaaga aacaacagtt cctaaggaaa aaacaaggaa agaggataaa ataactaata
    12781 gattagaaat ggaagaaata tttatgggag aataaacgtt ctaagaaact ttaca
//
LOCUS       DAYYME010000075        12794 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000075 DAYYME010000000
VERSION     DAYYME010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.076"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..1323
                     /gene="efmA"
                     /locus_tag="ACHBFW_001804"
     CDS             37..1323
                     /gene="efmA"
                     /locus_tag="ACHBFW_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFM9318108.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(1461..2060)
                     /locus_tag="ACHBFW_001805"
     CDS             complement(1461..2060)
                     /locus_tag="ACHBFW_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9318109.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            2464..3585
                     /gene="mnmA"
                     /locus_tag="ACHBFW_001806"
     CDS             2464..3585
                     /gene="mnmA"
                     /locus_tag="ACHBFW_001806"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFM9318110.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            3831..5252
                     /gene="arcD"
                     /locus_tag="ACHBFW_001807"
     CDS             3831..5252
                     /gene="arcD"
                     /locus_tag="ACHBFW_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFM9318111.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            5266..6384
                     /locus_tag="ACHBFW_001808"
     CDS             5266..6384
                     /locus_tag="ACHBFW_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFM9318112.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(6444..7736)
                     /locus_tag="ACHBFW_001809"
     CDS             complement(6444..7736)
                     /locus_tag="ACHBFW_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM9318113.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            7886..8446
                     /locus_tag="ACHBFW_001810"
     CDS             7886..8446
                     /locus_tag="ACHBFW_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9318114.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            8575..8952
                     /locus_tag="ACHBFW_001811"
     CDS             8575..8952
                     /locus_tag="ACHBFW_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFM9318115.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            8885..10957
                     /locus_tag="ACHBFW_001812"
     CDS             8885..10957
                     /locus_tag="ACHBFW_001812"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM9318116.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(11168..12115)
                     /locus_tag="ACHBFW_001813"
     CDS             complement(11168..12115)
                     /locus_tag="ACHBFW_001813"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9318117.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            12361..>12794
                     /locus_tag="ACHBFW_001814"
     CDS             12361..>12794
                     /locus_tag="ACHBFW_001814"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFM9318118.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
BASE COUNT         4119 a         2333 c         2730 g         3612 t
ORIGIN      
        1 acgctataca aaatgaaaaa gaaaagaggg atgtttatgg aaaatgaaca gtcggtcgtt
       61 ttaacgaatt ggaagagaaa ttatttgttc tttttatcgg gacagttttt gtcaggaatc
      121 actagtatgg tcgtccaata tgcgatcatc tggtatttga caagagaaac cggttcggcg
      181 acaattttga gttttgctac tttactagga atgattccca tggttttact aagcccattt
      241 gtaggaccgc tagtggatcg ctgggataaa aaagctcttt tgattgtcac ggatattatt
      301 gtagcgattt tcgcgcttat tttagcagta gtcggaacga tttcagaatc cttcccgatt
      361 tggctagttt ttgtgtcttt gtttatgcgt tcggtcgcac aaacattcca aatgccaacg
      421 atccagtcga ttatgccgac aatcgttcca tcttctcaca taacaaggac gaatggacag
      481 ttaggaatgg tccaatcagc gaatttcatc attgcgccag ctctaggtgc ggctttgttt
      541 tcagtcgttc ctgtcaatta ccttatttta ttagatgtat tgggggcagt ttttggtgtc
      601 ggcttattga tttttgtgaa gatcccaaaa gtttctcctg aaatattgga agttccgctt
      661 actattttta aagatgcaaa atttgggctg cagcaattga tggataataa agggttatgg
      721 tacataacga tcaatggtgc atttgtgatg ctgttgttca tgccagcaat cagtctgtat
      781 ccattgatga cgctagatta ctttggtggt tccgttggac aggcgggagc agtggaagta
      841 gtctacgcag tcggaatgct cttaggcggt gcactgatca gttttatagg aacatggaaa
      901 gaccgaatga aacctattat tatagcctac atcattatgg gcctgacgat cggtgcaagc
      961 ggattggttc caaacgatag tcaagggttc ttgtactttc ttatcttgaa tgcaggagca
     1021 ggttgtgcaa cgccatattt caatacactg ctgatggcaa tgatccagca gagttatgaa
     1081 tcgaatgttt taggtcgtgt cttagggaac ttcaactctt tgatgaacct ggcaggtcca
     1141 attggattat tatttgcagg accattagct gatcgtttag gggtagaaaa aatgttcttg
     1201 ttttccggaa tcgggatttt attatgcgga atcgttttat ttttaacgtc agctgcccga
     1261 aattatgata aagaattaca gaaaaaactg gtaaaagaac accatgagca aaaagacgaa
     1321 tagtatggaa tggagttgtg acagaagtct tgttacaact ccatttctga tcctaatgtt
     1381 cctgtggcaa taagcctact caaatgaaaa atatgagtaa ttatctttat ttgtttaata
     1441 cttgatatag ggcagttttt tcacgaccta tcgtacttcc tctttgcttc atagatttct
     1501 ttgatttgtt gctcagataa cggagtgcca aatggtagtt tttcctttaa gagttctctt
     1561 tccggtgcgt ctacgcctac attgacatcg ttctcgatag gtacgctgta atgatggata
     1621 tgtccatgaa gattgcggat agtgtcgtta ggcccgagca acatcggata atgagtcaag
     1681 taatattgtt gatggtcgaa tttaacgatg gcacctacat cataaaaata aaatttggga
     1741 cttccgtcag taagcctagg atcatttttt tctaaataac ggaaaaaagc gcgactgtca
     1801 tggttccctt tgatcaagtg gattgttcct tgcagttgag tgaccagttc caaaatttct
     1861 gcatttgctt tttcatattg cggatgcata gcaaggtcgc ctagatgata aacatggtcg
     1921 gtttctttta ttactgcatt ccagctagtg atcaactgtt ggttcatttc atccacattt
     1981 gcaaagagcc gcggagcaaa gtcgtttttt cctaataagc gttcatggaa aaagtgcata
     2041 tcactgataa agtaattcat gtatcttttg cctccctttt ttcttagagt aaacaaaatc
     2101 acttatctga gcaactcata acatctaaaa agaaaaggtt gtaaatgaga cgtgcagctt
     2161 taagcgataa gaagcaattt tgaaaaatag tttttcacaa tttataaaat tattccttat
     2221 tgccgaaagg ctggcccttt ttatgagtat gccaattttt tatatcgaat gctcaaaaga
     2281 tgagtgattt tttggttgac tatcatgttt gtgtactgaa taggtccagt agaacagtat
     2341 ttttacgact tgataattaa gggatgaaat tttcagaaat atgacgtata atgtaaaata
     2401 ctgaaaagtt gcgaccttca actcgtagat gtttcagaat ctaattgaaa tgatggtgat
     2461 atcatgacag acaacagcaa aacacgcgtt gtcgtaggga tgagcggcgg tgtcgactca
     2521 tctgtaacag cacttttgtt aaaggaacaa ggatacgacg tgatcggaat attcatgaaa
     2581 aattgggatg acacggacga aaacggtgta tgcacggcca cggaagatta taaggatgta
     2641 gcgaaagtag ctgctcaaat cgggattcct tattactctg tgaattttga aaaagaatat
     2701 tgggatcgtg tatttgaata tttcctagcc gaataccgcg caggacgtac acctaaccca
     2761 gacgtgatgt gcaacaaaga aatcaaattc aaagcattct tggattatgc aatggatctt
     2821 ggcgccgatt atgtagctac tggacattat gcgcaagtca ctcgcgatga aaacggcgtg
     2881 gtccacatgc ttcgtggagt agacaataat aaagatcaaa cttacttcct tagccaatta
     2941 tcacaagaac agctttcaaa aacgatgttc cctctaggcg gcatggaaaa atcagaagta
     3001 cgggcaattg ctgaacgcgc aggccttgcg actgctaaga aaaaagattc aacgggaatc
     3061 tgctttattg gtgaaaagaa cttcaaacaa tttttgagca actatctgcc agcaaaaaaa
     3121 ggaaatatgg tcactcttga tggtgaagtc aaaggacaac atgctggctt gatgtattat
     3181 acgattggtc aacgtcaagg actagggatc ggtggcggag gagattcgca agaaccctgg
     3241 tttgttgtag gaaaagatct agcgacaaac acattatatg tggggcaagg gttccatcat
     3301 cctgcattgt atgccacaag cttggatgca agtgaaatcc actttacgac gaatgaacca
     3361 atgccaaaag aattcaagtg tacagcgaaa ttccgttatc gtcagcaaga cgttcctgtg
     3421 acagttcgct tattggatga taatcgtgca gaagtcgtat tcgacgaacc tgtacgtgca
     3481 atcactcctg gacaagcagt tgtattttat gatggaatgg aatgtctggg tggtggtttg
     3541 atcgaccacg catatcaaga aacaaaagtt ttacaatacg tttaagcgat attaaatgaa
     3601 tcaaaacagc tatggctaac aaaagaaaca ccttttcttt tggatagcat agctgttttt
     3661 ttgcaataaa attaattttt attcatttta tacttgaaat acttgtgtaa aaagttcata
     3721 atatccaata atatgaataa agttgctttt tgttaagcaa gagcagttgc aaaatagaag
     3781 ggaatctttt gcaagtgatt tgttcaattt tataagtgag ggtgatggaa atggaaaaga
     3841 aatcgacggg tattagcaaa tggggactgg ttgctctggt tgtcagttcg tcaatcggaa
     3901 gcggtgtgtt tgggatcaca agtgatctgg ctagttctgc agctccaggg ccagcgatcc
     3961 tttcatggat aatcgttggg attggtattt tagctttggt attatcgctg aatcatttag
     4021 gagaaaagcg tccagatcta gatggtggta ttttcggtta cgcggaagct tcatttggta
     4081 aattaggtgg cttcatcagt ggttggggtt actggttgtc tgcgtggctg ggaaatgttg
     4141 cttttgcaac gatgctgatg agcgccatgg gagaattttt cccgatattc aaaggtggac
     4201 aaaatgttcc ttctatttta tttgcaagta tttttatttg gggtctgact ttattagtaa
     4261 acaatggagt tgaaggagcg agcgtcatca atacaatcgt gacgatctgt aaattagtgc
     4321 cattattcct atttttaata tttgtattta tcgcatttaa agtgaatata ttcactgctg
     4381 atttttgggg taacgtatct gataatctag tgaatggaac agggcagcaa ggaacgatct
     4441 ggttgcagat taaaggttgt ttgatggtta tgatgtgggt ctttgtaggg atcgaaggcg
     4501 caagtgtttt agccaaccgc gcgaagaaac gttcagaagc ccagcaagca tcgatcattg
     4561 gtctgctttg tttattgttt atctatatcc ttgcttcgat cttgccatac ggcgtcttat
     4621 ctcaagaaga gttagcaact atttcacaac ctgctatggc aaatatttta aaaggaatcg
     4681 tgggaaattg gggagcagcg ctgatcaata tcggcttgat tatttcgatc attggttctt
     4741 ggttgtcttg gacgatgctc ccagcagaaa caacgatgtt gatggctcga gacggtctgt
     4801 tgccgaaagg ttggggaaaa acgaataaaa aaaatgcccc aacttattcg ttagttgtta
     4861 ctgcaatctt gactaatcta ttcttattga ctttcttagt tactgattat gcctatcaat
     4921 ttgcctattc tttatgtaca gcggcaattt tgatttgtta tctacttgtc gggatctatc
     4981 aagtccagtt ttctttaaaa gaaggaatca aaaaacaagt agtgatcggt gggattgctg
     5041 tagtttttga attgatgggc atcattttag caggattttc ttatgtcctt ttatgtagta
     5101 ttgcctatct tcctggtttt tgcttctact ggatcgcttg tcgtgaaacc aaacatcaaa
     5161 ttacgggaaa agaaaaagta atgattgggc tgatttgtgt ggccgcactg ctttcaatcg
     5221 ggttgcttgc tgtaggctgg atcaagatat aagggggaaa gaaacatgaa aatcgcaaat
     5281 ttcggtgtag aagaatggct aaataaatgg gaaaagaaag cggtatatga tattgcacaa
     5341 agctcgatcg aagcattgac attagacgaa ctcgttggct tagatgggac tagcgtaagt
     5401 aaatttttcg aaaaacgcaa aaacgaacca ttggactatg gctggatcga aggatcagat
     5461 gttttcaaac aagaagtcgc ttctctttat caaaccgtcg atccggaaaa catcttacaa
     5521 acgaatggag cgactggtgc taatctttta gctctatacg cgttagtgga agcaggagac
     5581 catgttgttt cgatgcttcc tacttatcag caattatatg acatccccaa atctttaggt
     5641 gccgctgttg actttgttca tttgaaggaa gaagaagatt ggcagtttga tttagaacag
     5701 ctagaagcct tagtcaaacc tgaaacaaaa atcatctgtt tgaatagtgc aaacaatcct
     5761 acaggcacat tgctagatcg gaaaacttta gaaaaaatcg cagaaattgc tcgtacagta
     5821 gatgcatatg tattgataga tgaagtatat gcgcctttaa cggataaagg tgaattcttg
     5881 tcgatcgtag atgtctatga aaaaggtata gcaaccaatt ccctatccaa aacctactcc
     5941 attccaggta ttcgtattgg ctggacagct actggacctg agttggcaga agtttttcga
     6001 aagtatcgag actacacgat gatttgcggc ggagtcctgt cagatgatct tgctgtacat
     6061 gcattgaaga ataaagaaaa aatattggcg cgaaacaaaa agatcattac agagaacctt
     6121 gtgattttaa aacaatgggt agccaatgaa ccgaaagttg aacttgtcgc tcccaattac
     6181 gtctctactt ctttcattaa attagccatc gaagaagatg accagacatt ttgtatcaat
     6241 ttattagaag aaacaggagt actattagta ccaggaagtg cctttgattt accgaagcat
     6301 gcccgactag gttactgttg taaaaaagaa accttggaaa agggattatt cctcttatct
     6361 gagttcttga aaaaacaggc ctaaacaaaa acactgcaag gacagtctgt gacaactgtt
     6421 caagcagtgt ttttcattta cgcttattgt ctatgagaag gagagtgccc tgaagctatg
     6481 gagactcctg aagctttttc ttctgcagtt tcttgctgat ggatctcttg tgtattgtgt
     6541 cgtacaagca agaagttgac caaaacaaga agagaagtcg aaagaaagac gccgcgataa
     6601 ccaaatgcac tggaaacact ggagccgatc ataggcccta ctacgttacc agttgcttgg
     6661 aaagattggt tataactgaa gatccgtcca gcagcttgat gaggcgaata ttttgtgatc
     6721 aatgcttgga cagcagggag cagacaagca tcagagatac caatcaaaaa acgtaaggca
     6781 gctaattgcc aaacattcgt gacgaatgcc attgggatat aaaccagaat cgcaaaaccc
     6841 aaaccaatcg caagtattct ttcactccca atcttgtcac ctaagcgacc aaatcttgga
     6901 gcggctatca aagtggcaat cccaggtatc gaagcaatga ttccgctgat caacgtaaca
     6961 ttcccatgtc catgcagcaa ttggcgaatg tacagactga tgattggact aatggaattg
     7021 ttggatgctt ggatgatcat cgttgtgacg aacataccga tgacgacgtg tggatatttc
     7081 aagtctttga aaatctgttt ggcagaaacc atgttttctt ttttcactgg gacaaatttt
     7141 tcatggacga aaaacagact caacaagaag acgaaaaata agatgatccc tgtaatgaaa
     7201 aaggtagggc gatagccaaa cgctgaagca gagatcccgc caagaagcgg tccaagtaat
     7261 gttcctgtaa cagaaccagt tgctaaggtt ccaagaactt gaccactttt ttcttttggc
     7321 gtcccagttg cgacaagggc agtggcatta ctgatataac cagaaaaaat cccttgaagc
     7381 agacgcaatg cgactaattg atacacacta gtcacgcaac ccatcaaaga aatgacgatt
     7441 gccattccta atgaagcacg caacagcatc aattttcttc ctttttggtc ggctaatttt
     7501 ccccaccaag gagagataag cgtagtcacc aagaaagtag atgaaaaagt caatccgctc
     7561 caaaagttca attgtgacgt actataatta cctaacgtat ctatgtaaag tgacataaaa
     7621 ggcatgacta aactaaatcc gataccagcc ataaatgttc caaaccataa gacaaacaag
     7681 ttttgttccc aagtttctct tttcctaaat aatttttcct ttattcttgc agccaattag
     7741 actccttctt tctgaaaatt ttcatctaca gctctttatt ataaactaca atatgtagtt
     7801 tatcaatgaa agtctctttt ttctctttct ttttcgtggt aaactgaaag agtgaaggat
     7861 ttattttatg agaggaaaca tggaaatgaa aaaaagaaat ctgtctcaag agaaaatcat
     7921 cgactgcttt cgagaactag cagaagagat ggatgttcag caaataacct ttcagcattt
     7981 agccaaacgc ttggatatca aggctccctc gctgtataac catttcaaaa atattcgtga
     8041 tgtcaaaaca gcgctaacag cgaaacttct gcaggaactt aatgaccagc tgcgacgggc
     8101 tcttgtggga aaaagtggtc aggaagcact gcagatctat gcagaaactt atcgatcctt
     8161 tgctttttca aatcaagcag tgtatgagtt actgatcagt gtaccgcata ccaatgaagc
     8221 tgttttatta gatggaatcc atgaaacgaa tcagatcatt ttgcagatat ttgaggcttt
     8281 tcaattaacg aaagaagaaa aagttcatcg aagcagggaa cttcgtagta tgatccacgg
     8341 ttatctttcc ttacgttttt taggatactt tacaaaagaa ccaacaatcg atccagaaga
     8401 cagctatttt tggatgatca ataactttat cgctactttg ccttaaacaa tcagattcat
     8461 ttgtaacaat tttaagatgt tcattaaatg acccttttat aagagaaaat tcttgcaact
     8521 ttcttgaaaa gtgactaaga tgagtctaga aaagctgaaa gaaggagcac gagtatgtat
     8581 caagtaatta ccatgttcgg ggacaatgag ccttggtggt tttttgaaga ctgggaagaa
     8641 gatattgaag aagaagaaac attcgagtct ttcgaagaag cacgacaagc gtacgaaaaa
     8701 caatggaacg aaatccatca aaattatgaa tatatcaatg caaaaagtaa ctttctcagc
     8761 gccttttgga atgacggaga tgagcgctgg tgtgaagagt gtgacgatga tttacagcaa
     8821 tacaagggac ttgcgttgtt gaaaaatcat cagcccatca cagtagaaag cagaaaggaa
     8881 ttttatgaaa caactaattc tagcggaaaa accaagcgtt gcgaaagacc taagcaaggt
     8941 gcttggtgct aatcaaaagc acaaaaatta ttatgaagga cccaaggtga tcgttacttg
     9001 ggcccttggt catttattag gattgaaaat gccggaagat ctgaataagg aatggcagag
     9061 ctggcaaatg gacacgttgc caatgatccc taaaaaacta ggaatcaaac cacttcctaa
     9121 aacaggacat caattaaaag caatcaagca gctggctcag cgaaaagatg tctcagaagc
     9181 agtcattgcg acagatgccg gaagagaagg ggaacttgtt gctcgctgga tcttagagta
     9241 tgtgcatttc aataaaccag tgaagcggtt atggatctct tctcaaacag ataaagcaat
     9301 caaaactgga ttcaagcaac taagacctgc gaaagaatat gatgcattgt atgaatctgc
     9361 tcttgcccgg gcaaaagcgg actggctggt aggattgaat gtgaccagag ccttaactgt
     9421 gaagtacgcg gataatttat cagcaggccg tgtgcagaca ccgacactag cattagtgag
     9481 agaccaagaa aagaaaatcg agcagttccg tccacaaacg tatttcacga tcacgttgca
     9541 agctggaaaa gaacaagcca aaatgcttca aaaaaatcaa tttgctttaa aaacacaaga
     9601 agaagcacaa aaacttgttc aattgatcag tacaagcaaa ggaactgtca cagaagtcca
     9661 agaaaaatta aaaacggaat ctgcgccatt gccttatgat ttaacagaga tccaaagaga
     9721 agccaatcaa cgctatggct attctgcaaa gaaaacatta agtcttgtac aaagtttgta
     9781 tgagacccat aagatcgtta cttatccgcg tacagacagc aaatatctga caaatgacat
     9841 gaaaagtacg atgaaagaac gattgcaagc agtcagcgac tttgctcctg aggtcaagca
     9901 gtatttgaaa aacggagcca tcgtccaaca gacaaaagta ttccaagaca gtaaagtgac
     9961 cgatcaccat gcacttttgc caactgaaaa cagagcacgt tatgaaaaat taagtaatga
    10021 agagcgaaaa atctatcaaa tgattgtctc tcgtttttta ggactgtttg ctcaacccca
    10081 taaagtcagc caaacaaaag ttacggttga atttggtaaa gaacgattca tttttcggca
    10141 aaatcgtgtc gtccaagcag gatggaaagg cgaaactgaa actgagacgg aaacagtcaa
    10201 atgggaaaaa ggaatgagaa tcaatcccga tttcgccatc aaaaaagaac tatctgcacc
    10261 gcctaaacca ttgtcagaag caagcttgct tggtcaaatg gaaaaataca gtctgggaac
    10321 cccagctacg cgtgcagaga ttatcgagaa actaatcaaa tctgaactga tggaacgtac
    10381 ccctcaagga ctgcaagtat ctcctaaagg taagcaatta ttagtattgg taaatccctc
    10441 tttagtaaca ccagatttga cagaaagctg ggaaaaagac cttgaagcta ttgctgcagg
    10501 aaagaaacaa gcaggaacct tcttgaatgg aatcgaaaaa gaaacaaaac gcttggtgaa
    10561 tgagatcaaa tcaagcaaac aagaatatca agatttctcg attacccaaa agaaatgccc
    10621 agaatgtggg gccaatctgc gcgagaaaaa cacacgtgac ggcaaaatct atgtctgcac
    10681 caattcagat tgcaactacc gcagaagaaa agatccaaaa gtctctaatc atcgatgccc
    10741 gcaatgtcac cggaaaatgg aaatcatcga aggtaaaaac ggtgcttatt tccgttgtaa
    10801 atacgatgga acgacagaaa aattgcttga taaaaaagag cagaagaaga aaatgaccaa
    10861 acatgaagaa cgacgcttgc tgaaaaaata tgcacaagca gaagaaccag aagaaagccc
    10921 attagcagca gcactaaaag cagcaatgaa gaagtaataa gatatgaaag aggttgtgat
    10981 agaagtggtc agcttcaaga aataagaagg aatttacgaa aattgcttct caaatttttg
    11041 tgaaatttcg gcttatttcc gaagaagcta cttctggaac accatttatt cggaaaaaag
    11101 gactgtgaca agacttttgt cacagtcctt tttcatttaa aaaaatgttt acttcatttg
    11161 acgtatatta ttggtgaggc ttcaattcat ttggcttcac tttttctgtt tctcttatga
    11221 tataaacagg acgctgtttc gtttctaaga agattttccc aatatatttc ccgatgattc
    11281 caagacataa caattggatt ccaccaatga acaggaagat cgagaccatg gaaggccatc
    11341 ctgatgtagg atcaccaaac aacaatgccc gcaaaataat gaacaaaatt gcaataccag
    11401 aaccgacaca agacaaagcg ccgataaatg aagcaatcgt taacggtgca tccgagaagt
    11461 tcacgatacc atcaatcgag tagttgaata atttccagaa ggaccaagat gtttcgccag
    11521 ccacacgttc gcggttttca taagaaatat attctgtttt aaatccgacc cagctaaata
    11581 atccttttga aaatcggttg tattctgtta attctaggac ggcatccacc atttgacgag
    11641 tcatcaagcg gaaatcacga gcgccatcga ccatttctgt atcgctgatt ttattgatca
    11701 actggtagaa ttttttcgca aagaaactgc gtatcgcagg ctcgccttca cgagtcgaac
    11761 gccgggtgcc gacacaatcg atatcagaag tagtgatccg gttgatcatc tcaggaagca
    11821 gttcgggcgg atcttgcaga tcaacatcca tcacggtcac taaatctcct gtagaagctt
    11881 gcaatccagc gtacagagcc gcttctttcc caaaatttct ggaaaatgag acaaagcgga
    11941 cataaggata agctgaggct agcttcttga ttgctgctaa cgtaccgtct tttgatccgt
    12001 cgtcaataaa caaatattcg aactcatggg cacctttgaa tttttcaacc tcatcaaaaa
    12061 aaagaggaac agttttttct tcattaaaac atgggacaac aattgatact ttcatcgttt
    12121 ctatcctttc aatcgtatgt ttatatatgg tttacattca aaatttaata aaccttaaat
    12181 aacaccttta aaatttgatt ttacgtaaaa gaacaggtat tctttttgta aactaacgta
    12241 tttagtttac catgtttcct cggtttatca aacttttaag aacaattttt attagttggc
    12301 aagtagtttt ggatagagaa aaaggaggaa gttccagtct attgactagg agcaaacaaa
    12361 atgaaaatac acacacctat cggcttttta tggttagatg cagatgaatc cggattgact
    12421 gctgtatctt ttcagccaat ttcagaaagc gaatcagcga atcagcgaat tttaacgcgc
    12481 acacaagaac agtttgaaga atattttgcg ggaaaaagaa aagaatttga tgttccttta
    12541 tctataaaga gaggtacgcc ttttcagcaa aatgtctgga aagctttgtc gaccattcct
    12601 tatggagaaa tccgaacgta taaagaagta gccgttgctg ttgatagtcc gaaagctgtg
    12661 cgagcgattg ggcaggcaaa tcgaaccaat ccattgccaa tcgttattcc ttgtcatcga
    12721 gtgatcgggc aaaatggaaa actgacagga tatatgggaa acgcaataga tggggttgaa
    12781 atcaaacgcc aatt
//
LOCUS       DAYYME010000076        12736 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000076 DAYYME010000000
VERSION     DAYYME010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.077"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(87..266)
                     /gene="rpmF"
                     /locus_tag="ACHBFW_001815"
     CDS             complement(87..266)
                     /gene="rpmF"
                     /locus_tag="ACHBFW_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM9318119.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(324..893)
                     /locus_tag="ACHBFW_001816"
     CDS             complement(324..893)
                     /locus_tag="ACHBFW_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFM9318120.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1079..1981
                     /locus_tag="ACHBFW_001817"
     CDS             1079..1981
                     /locus_tag="ACHBFW_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFM9318121.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="ACHBFW_001818"
     CDS             complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="ACHBFW_001818"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFM9318122.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="ACHBFW_001819"
     CDS             complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="ACHBFW_001819"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFM9318123.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="ACHBFW_001820"
     CDS             complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="ACHBFW_001820"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFM9318124.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8549..8734
                     /locus_tag="ACHBFW_001821"
     CDS             8549..8734
                     /locus_tag="ACHBFW_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFM9318125.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8796..9992)
                     /locus_tag="ACHBFW_001822"
     CDS             complement(8796..9992)
                     /locus_tag="ACHBFW_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM9318126.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10162..11598)
                     /locus_tag="ACHBFW_001823"
     CDS             complement(10162..11598)
                     /locus_tag="ACHBFW_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM9318127.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11891..12700
                     /locus_tag="ACHBFW_001824"
     CDS             11891..12700
                     /locus_tag="ACHBFW_001824"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9318128.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2673 c         2250 g         4010 t
ORIGIN      
        1 atatttttta tttaccgact acaaaaaaat aaacctcaag gctccatggt tagccttgag
       61 gttttacatt ttctaattaa aatgtattaa gcttcttttg acattacgtc cttaccatca
      121 tagtaaccac aatttgcaca tacgtgatgg ctttttttca tttcaccaca gtttggacat
      181 tcgtttaacc ctttgattgt caatttgtag tgtgtacgac gttttgcttt tttagctttt
      241 gatgttcttc tagctggtac tgccattttg ctacacctcc ttgtaaagtc ttgtatctaa
      301 attagacaca atatattcca atcttacttg ttatcgtcgt cttttgattt agaatcaaaa
      361 agttcagata gttttgcaag acgaggatca attttattct cggcttcttc catttttctg
      421 cgctggtatt cttcttcgga aacaacttcc caatcattgc cttttggaag ttctgttgct
      481 gcttcctctt cttttgtcaa tacccgcatt ggaatcgcca ataagatgtt gtcttcaatc
      541 gactctgcta aattcaataa tggaccttca attagcaaaa tctcatctgc agacatttgt
      601 tcatctctac tttggaattg ttcttctgtc atgaatactt cgtctacaga aaaatccatg
      661 ggcagttcaa caggttctag tgaacgtgtt gaaggaactg tcaaagtcgt ctgcacagtg
      721 taatgaacga tatagccttt cttatcaact gagaccaaac cttcgacttc cactggcgca
      781 acatcaagta ttaaattgtc tcgcttcatg agagcggctt tcaagtcgag cgtctcatga
      841 aaagttaacg gtgtctcctg gtattttctc aattccaata aagaccattt catttttcat
      901 cactcctaag caacaaaagc aattatacag ggcaaaaagc atgttgtcaa tgaaattttc
      961 ttgacacctt gcagttcata aaaaaacaga aaaatcttta ttagaaagcc tctgtttgct
     1021 atcatcctca gaatcattta caataatcat tattccatta aaggagcgga tgatcttcat
     1081 gaacacgaaa gatatcgaat actatgtcca aatcgtcaaa caaaaaaatt ttacgaaagt
     1141 ggctaattat ttccgtgtga gccaacctac tattacatat gcattacaac gtcttgaaaa
     1201 agagtttgac accgtactta ttacaagaga tcgatcgcat cacgaattaa tcgtcacgcc
     1261 ttctggaaag cagctgtttc tccatgcaca aaccattctc caagagattt ctactgcaag
     1321 agctgaaatc actcagttgc aaaagaaaaa gcttcgtttt ggcatgcctc ctattatcgg
     1381 caatgaatac tttcctaaac tgtcaagtta ctttatcaag catgaattga tgaatcaaat
     1441 tgagattacc gatggaggct ctcgggactt atacgggatg atccgacaag ggaggatcga
     1501 tttagctgta ttaggctcaa cacagccaat cgtggatcaa tatttagaaa cagagttact
     1561 tttggacaaa cgtttcatga tcgtcgtgcc acctacacat cctctttcca agcaggatac
     1621 tgtctctttc aaagatatag cagatgaaca gtttgtacta ttgaacgaac attatgtcca
     1681 ccccgcagca ttcactaagc tttctaaaca aacacatact catccaaaag ttgtttatca
     1741 aagcaatgac ttgacgattt taaagagtat ggtccgagaa aatgtgggta tcggtttttt
     1801 ggcagagatt gctgtccatc ctgaagatca tttagtttgc ctccctcttt cagatgaagt
     1861 tcagcctcgc ttcttgattt cattggcaaa acgaacacag cagatcgtca cgcctcttca
     1921 agaacaagta gtccaagtta tccatgaatt cgttacagca caaaaaaaca gctcttcgta
     1981 aaagaagagc tgtttttatt ttttattaag ctaaattaga tagctgttgt ttcgccacgg
     2041 taaacgatgc cgcgacgtga atcaacggta atcaattcac cttctgtaat tgttgttgtt
     2101 gcgcctttag caccaacgat tacaggaata tcttgtgcaa ttcctacaac tgcagcgtga
     2161 gaagtcaatc cgccttcttc aacgataaca gcagctgctt tttcgattgc aggcatatat
     2221 tctttgtcag ttgatggtac aactagaacc attccgtctt ttgctttttt tacagcttct
     2281 tcagcagatg aagcaataac tgcatttgca acgacagatt tttgtccaac gccttgagct
     2341 tctaataatt tagaaccaat catttgaatt ttcatcacgt tagttgttcc gcgttcacca
     2401 actggtacac cagcagtgat caagattaaa tcgccttctg atgcgaaacc taattcaaca
     2461 gcttttttag ctgctagttc aaacatttca tcagttgttg atggtttttc agcaactgtt
     2521 gggtaaacgc cccagtttaa catcaaacca cgttttgtgc gttcgtcaaa agtaacagcc
     2581 aagatatctg catctggacg atattttgaa atcatttttg cagtgtatcc tgattcagtt
     2641 gcagcaacga ttgttttcac gcctagattt tttgcagcac gagctacaga taaaccgatt
     2701 gtttcagtta catctgtttt atcgaattcg ttcaattgga atgtacctaa ttctgataat
     2761 gcattttcag cttcttggtc gatacgtgcc attgttgaaa cagcttcaac tgggtagtcc
     2821 ccgtttgcag attcaccaga tagcattgtt gcatctgtgc catcaaatac agcgttagca
     2881 acgtcagaag cttccgcacg tgttggacgt gggttttctt gcattgattc taacatttgt
     2941 gtagcagtga taactgggat accagcagca ttacattttt tgataatgcg tttttgaacc
     3001 attggtacta gttcagctgg gatttcaaca cccatatctc cgcgggcaat cataacaccg
     3061 tcagaaactt tgatgatttc gtcaatgttg tcgatacctt cttgagattc gattttaggg
     3121 aagatttgaa catgagtcat gtctttttct tctaagattt cgcggatatc tagaacgtct
     3181 tgtgctttac gtacaaaact tgcagcgata aagtcgatat cgttgtctaa accaaaacga
     3241 atatcgtcag catcttttgg agtaatacct ggcaaactaa tagaaacgcc tggtgcattt
     3301 acccctttac gtgatcctag catacctgca tttttaactt ctacgactaa ttcacggttt
     3361 gcttcgtctt tttcaatgat ttgcatatcg atcaaaccat catcaaataa aatgtgtccg
     3421 ccaacgtgaa catcgtcaaa taatcctggg taagtaaccg cgattttttc ttttgaacct
     3481 acatgttcag gatccataga gatgcgtgtt tgatctccta cttcaaattt gatgtatcct
     3541 gcacgaccaa aatcagcttc tgttgtgcct tgaacagttg tacggatttc agctcctttt
     3601 gtatctaaaa gaataccgac atcttttcct gtctttttaa ctgcttcacg gaccatagtc
     3661 atacgtgata attgttcttc atggtcacca tgagaaaaat taaagcggaa gacatttgcc
     3721 ccagattcaa tcaattgaga aataatttct actgagttac ttgctggtcc taatgtacta
     3781 acgattttgg tttttttcat ttaaaaaact ctcccattca attttgttta tttaaagctg
     3841 tacacagctt aaatagcttg gtttctttca ctcttaaaat gaaagtgaat tatttagatc
     3901 atatagtgat aagtctggtt tatgtttgtt gttttcaagt gtatcaatga tgtcagacgc
     3961 aacgatttcg ttgtcatgga taccgacaca taatccacct tttccttctt gaagcaattc
     4021 gacagcgtaa gcaccgaatt tacttgccaa cacgcggtcg cgtgcgcttg gtgctccacc
     4081 acggacaacg tgtcccaaga tagatacacg tgtatgatag tcaccaaatt ctgaaagttt
     4141 ctcagcgaat tcatttccgc ccattacacc ttcagccaag atgatcaagc aatgtttctt
     4201 cccgcgatcg cggccttctt ggattttttt agcaactgaa gccatatcaa aatcatgttc
     4261 tgggatgatg atttcatccg ctccgccagc cacacctgac cataaagcga tgtctcctgc
     4321 attgcgtccc attacttcga taacgaatgt acgaacatga gaagtcgctg tgtcacgaat
     4381 acggtcaatt gattctagta ctgtattgat tgctgtatca aaaccgattg tgaaatcagt
     4441 cccaggaata tcattatcga ttgttccagg aatacctact gctgggaaac cgcgttttgt
     4501 caaagccatt gcgccatgat aagagccgtc tcctccgata accactagac cttcgattcc
     4561 gaattttttc aattgttcga ttccttttaa ttgaccttct tcagttgcaa attctggata
     4621 acgtgcagaa tacaagaatg taccaccacg ttggatcttg tcacctacat ctgccacatc
     4681 aagacgacga atatctccag caactaaacc ggcaaaacca taattgatcc cgtatacttc
     4741 cagtccttcg taaatacctt tacgaacaac ggcacgaaca gctgcattca ttccaggtgc
     4801 gtctccgcca ctggtcaaaa ttccgatgcg tttcataata aacattcacc tcattaaaat
     4861 actttatcct aagtatgact taggtttaca accatttttg cgttaaccac gactaacatt
     4921 ctaacattat ttttctcact tgtcttgatt tacggataaa aaaatgaaaa aaaatgaaag
     4981 caagtagtat tttttaccgt aagttatttg aaaaccacat tatcttcgcc aagcagataa
     5041 ataagttgcc cctttatact cttgctgtca tccacccaat aagcatctga cagaactact
     5101 tttcgttgcg ttttttcgtg atacataact actggtatat atcctgggtg tttctgaaaa
     5161 atcacttgta attgtttcat tatctcggga tcttcccgtt cttctgtaat acgcaaatag
     5221 caagtttgtg aaggatactg ggcttctaac tggttaggat cagcaaagct ttctccaatt
     5281 agttgcagtt cctgattata tttgctgatt tcgctccgcc cttgaacaaa gatcactcga
     5341 tcaacggcaa acaaattacg atatttacga taaagagttg gaaaaactgt gacagaaatt
     5401 tctccactac tgtcggttcc ttcaacaaag gccatcagtt ctcctttttt tgtccgaatt
     5461 tctctaactt ccttgatcat cactaataaa ttgactggtt gattagggac gatatctgta
     5521 atcagctgaa cttgtttagc taaacgaaga cgatcaaacc cttctgcagg atgacctgac
     5581 aaataaatac ctaagtactc ctcttccagc ttcaagcgtt cttctagtgt atagtcagcg
     5641 acttcttctt cttttaatgc catgattccc agcaagtcaa gacttccacc actgtaaacg
     5701 atattttgga tttttccttc gagatcaagc atcaattgtt tcctgttagg agataattca
     5761 tcaaaaactc ctactgctgt cagtgattgc agtaactcta atttcagcca acgcttatcg
     5821 atccgtatga ggaattgatc cactgactgg aacgggccat tttgttttct ttcgtttaat
     5881 atatcttcta caaaatcacg ccgaatacct tttatcgctg tcagtccgaa tctgatttct
     5941 tccttattga ccaactcaaa actggaatta ctttgattaa tggacggcga ggaaagtttg
     6001 agtttatttt tacgtgcttc tgcaatatat tctttcaatt ttgatgtgtt atgccgaaca
     6061 gaattcatca ggctggtaaa gaaagcacct ggatgatggg cttttagata cgccatttgg
     6121 aatcccacga atgaataagc aaatgcatgt gaccgattga atccatagtt tgcaaatttt
     6181 tcgatatatt catatacggt cgctgctgtt ttttccgaat atccctgttg taatgctcct
     6241 gatacaaaat gcgacttttc ttcatctaat acgtcttttt tctttttact cactgcccgt
     6301 cgcagaatat ctgactgccc taatgtaaat ccagccatag tcgaggcgat ctgcatgatt
     6361 tgttcctgat agacgatcac tccatatgtg tttttcaaaa tcgggatcag tgaatcatct
     6421 gggtagcgaa tggcttcttt tcctttcttc ctggcaatga aggtatctat attttgcata
     6481 gggcctggtc gatacaatgc attgactgcg gcaatatctt cgatgttttc tggatctaat
     6541 cttcgtaaaa cgttccggat accagcagat tcaaattgaa atactcctga ggtttcgcct
     6601 cgttgaaaca agagcagcgt ttttgcatca tttagcggga tctcttttaa tcggatctca
     6661 tacccttcta ctcgcttaac agctttcaaa gtaaaatcaa taatcgataa gttgcgtagt
     6721 cctaaaaagt ccattttcaa aagaccgatt gcttctacct cattcatcgt atactgggtc
     6781 agaaaagctt catcagaacc gcgttggagc ggaactagtt caagtagatt cttatcgctg
     6841 ataactacac ctgctgcgtg agtcgaaaca tgtcttggca atccttctaa aatcactgca
     6901 gttttgaata aaagttggtt tttttcgctt gcctgaacta attctactaa ttgatgcgaa
     6961 gttttataag cttgggtcaa tgtgattttc aattgattgg gtattgcttt tgaccagcgg
     7021 tttgcttcac tttgcgataa accaaacact ctagctgcat ctctaagaac catttttgct
     7081 gccattgtgc caaatgttgc gatttgtgcc atatgatatt gtccatactt ttctcgaaca
     7141 taattcaata cttcttcacg tcgattgtca ggaatatcca aatcgatatc tggcatcgta
     7201 aagcgttcgg ggtttaaaaa gcgttcgaac agcaaatcat attctaatgg atccacatct
     7261 gtgatttcta acacataggc tactaatgaa cccgcagcag aaccacgacc tgcaccggta
     7321 acgattttgt ttctatgagc aaaagccatt acatcccata cgatcaagaa ataatcatcg
     7381 aatcccattt gatggatgac agataactcg tagtccaaac gttcttggta ttcctttgta
     7441 tagtcagtga ttcggcgttc cacttgcgaa tagcataact ctcttaaata ttctccagct
     7501 tgttttccat taggaactgg ataatgcgga agtaatttct gatggaactg gaattcaaat
     7561 gaaatcgatt cagctaaacg ctgggcgcct attacagctt gggaaagacc cgctttttcg
     7621 aatcgtttga tcatttcttg ttctgaaaga agatagacat ttgctgttag tggtaaagat
     7681 gcagataaat tttctaaagg tatttggctt ccctctttga tatgtttcat cacttcaagt
     7741 gcaaaaagtt cttcttgttg taaactagcg atgggatgta atgcacaaag cgaataattt
     7801 tcttttatca catactgata ccattcaggc aattgatcag gcgtgttttc tgtcaagccg
     7861 atataaaaat ttctagtaaa aacattttca agtaaaaact gccaatgacc gaaaaagtta
     7921 tcctctttat tgaaagcctc agaaacagaa tgatttgccg gaacaacaaa cgctagatgg
     7981 tcggtttctg tcatttctgc aagttccaat ttttcttgga tcatttttcg gctggaaagt
     8041 ttcatcagct gctgataacc tatttcatct tttgcgtacg caagcaagcc ataatttttt
     8101 tcttcataag tataactaag atgtaatcca atgattggct gtactccttc ttctacacaa
     8161 atacgataga attcaggtgc accgtataag acatctttat ccgttatggc taatgaagtg
     8221 tagccttgtt tttttgcttc ttgtacatat tctcgaatac gtatcgtact ttgtaaaaga
     8281 gaataagaag taattgtata aagttgtggg aaaaacatct tgcattccgc cttttttttc
     8341 gttaaaattc actagcgtta aaaggtttga tccttttact ttcattaaga tagcaggcgc
     8401 tgaagttgat tattccactt catttgcttg cttgtaatct gtaggaatga gttctttcgc
     8461 aagaactcaa attttattga gatttggtct cgtttcttta cattcaaaga aaatctggta
     8521 aaattaagat agaaaagagg tgttctttat gcgatatatc gttacattat tctgggctgt
     8581 cgttctaggt caagtagtcg gttatattgg tgctgcatta acaagcggaa catatgattt
     8641 tactttgaca acaatcattt cattcattgc gggtgtcatt atccttttga ttggtgcagt
     8701 tgcgccaaga aaagaaacct ctgcccattc gtaaaaaaac agccaagagc ttgagatttc
     8761 tcaagctctt ttttattttc tactcctcac caccattata atctgctgcg aagaacttta
     8821 cgaaagttga ataaagaaag aagaatattt gacagaacaa acaaacttgt cacaataaaa
     8881 acagcactgt aattataagc atgcgcgact gtcgatccta gcatgggacc taaaacttgt
     8941 ccaaagttgc taaacatctg attgtagcta tagattcggc tgactccttc ttgaggcgta
     9001 atcttactga tcagtgtatt gacagaaggc atcaaagcgc cagttgaaaa gcctaaaata
     9061 aagcgaagta tacctaattg gagcggtgta gtgacgaatg ccataggtaa ataacagcaa
     9121 aatgataaaa tgagtcctcc aattagaact ttttgattcc caatcttatc ccctagtttt
     9181 cctagtttcg gtgaagaaaa gacagcggaa acaccagcta tcgaaacaat caagccgctg
     9241 acaaaaagga tattttctgt acttccgctc aattcacgaa tatataaggt caaaataggg
     9301 ctgatcgtag tgattcctat ctgtaaaatc aaggtcgtaa tgaagagacc gatcaaaata
     9361 gagagatgat ccatttttga aaaaatttct ttggtgctta tcagatcttt tttctcgatt
     9421 ggatggaaat cttctttgac taagaaaatg gtcaataccg ttgtgatcat caacaaagcc
     9481 cctgtgatca agaatacatt ttccatacca aaccactgag ccagtgcacc gcccattgaa
     9541 ggtccgatca gatttccagc aatcgctccg gtagacaatg ttcctaacgc ccatccgctc
     9601 ttttctctag gtgcctgtga agcaatcatc gctgtggcat ttggaatata tccagacaaa
     9661 atcccattcc aaaaccgcat gatcaaaagc caatacgcgt ttggtacaaa agccaaagat
     9721 cccattgtta atgtcatccc tgctgctgca cggatcatca tgagttttcg gccttttcga
     9781 tcagctagat ttccccagat tggtgcaacc acggcagatg caaaagcagt cacagagatt
     9841 gctagtcccg aaaacaattc gatttgatct tttggtgttc ctagttgctc aacataaact
     9901 ggtatgaatg gcataactaa actaatactt gcaccagtga aaaaacagcc aatccacgca
     9961 atcactaaat tttttctcca attaatctcc atctatcatc cctctttcgt ctcttttatc
    10021 atacctattt tcgaataaac aagcaacgat ctcgccagta aaaagagaaa aatcccgcaa
    10081 aggtcatttt atatgtaacg ttctttttat gtaaaacaaa aagagatcag aacaaatact
    10141 gttccaatcc cttctcttta gttaaattgt tgctagaatg atgaagttga taatgaataa
    10201 aatatcaacg atccacaaag caacagaaac ttgattgtat tttcctttga ttactttcac
    10261 aattgtatag aaaataaatc ctgctgcaat cccataggaa atgctatagc acaatcccat
    10321 aaagatcgat gcaaagaagg ccggtaaagc ttcttccata tctagccatt tgatgtctgc
    10381 aaaagaagcc atcatcatta ctccaactaa aatcaatgct ggcgcagttg cttgatttgg
    10441 tacaatggcg atcagtggag agaataaact acttaatgcg aatagcactg ccactactac
    10501 tgaagtcaaa ccagtacgtc caccggcccc gatcccagct gcagattcga cataagttgt
    10561 tgtattcgat gtaccaaaga tagcccccac agaagtagcg atcgcatcgg caaatagtgc
    10621 tttatccatt tttgttttga acccacgacc atcttccaac gcgatctcgt cttcttttga
    10681 gaaaatacct gtgcggcgtc ctgtaccgat aaatgttccg atcgtatcga aagtatcaga
    10741 taaactgaat gcgatgatcg tcatcaatac ttgaggaatc ttggaagaat cactgaataa
    10801 agattgcatg ccttcagaac caaatgctgc accgaaagtt gttcctaatt ctttaaatga
    10861 attacctaac gaattcgttt gccaatcaat ggaacttaaa tcaacaacac ccataaaaat
    10921 accaaggact gtggtcgcta cgataccgat aagaatagcc ccacggacat tttttacaac
    10981 taaaatggct gtcagtacca aaccaatcac agccaataag ataggagcgt tgtcaaagtt
    11041 ggctaacgca gggacgattc caccatttat cgtaacattt gtcgcttttc cgccttcaac
    11101 gacagagctc gaaatagctg actggtctgc tgaaaaagat aagaatcctg catttttgat
    11161 tccgacatac gcaacgaaaa taccgatccc gccaccaatc gcatgttgaa gactttcggg
    11221 aatcgcccga ataatcattt ttcgaatatt tgtgacagta ataaaaatat tgattaatcc
    11281 gcaaataaac accattgcca aggcttgttg ccaagaataa ccaagcccaa aaacgaccgt
    11341 aaaagtgaaa aatgcgttca atcccatacc aggtgcctga gcatatggta catttgcgaa
    11401 caatcccatt accagcgtac caataatcga tgcaatgatt gtcgctagaa atactgcttg
    11461 gaagggcata cctgatgctg acaaaatcgt cggatttacg aataagatat aactcatggc
    11521 aaagaatgtc gttactcccg ccattatttc agttgataca tttgtattgt ttttctttaa
    11581 accgaaaaac ttttccataa aaagttccac tccctgaatt tttttctgat ggaaaactat
    11641 aataataaaa aataagtaaa atagggcaaa tccatcgaac aatttcaaat tatatagacg
    11701 tttcattcgc ttttcaacgt ttttttaaca aaaaaagcga aaataatact taacgttcgt
    11761 gtttagctta tgtttagcta tttatatagc cataactgta gtttttgtta ggaatagctg
    11821 attgctcttg ttaattacct aaaatatgct agactaaaac aaatgctagt tgaggaagga
    11881 aggaccctta ttgaataaaa aattaattgc catcgatctg gacggaacta ccctcaatca
    11941 agattcgatg attacatcta aaacaaaaga gactctaaaa aaagcaatca atgccggtca
    12001 tcatgtcagt attgctacag gacgcccatt ccgaatgagc catcagtttt accaacaatt
    12061 agaattaaca acaccgatgg ttaattttaa cggcgcatta gtacacatcc ctaatcagca
    12121 ctgggatggt gaaagagaaa cgttgatcaa tcgtgaaatc gcttttgaaa tcttatcgca
    12181 aaaaaagcag ctaaatttag attttatcgc tgctgaaaat cgtgatactt tttttattga
    12241 cagttttgat ttctttgatg aaaaaatttt tgcttcttct cgtccaggag agaagaattt
    12301 gctttctcct aaaaatttga cgacaaatcc tacatctttg cttgtccgaa cagacaaacg
    12361 ttttgcagga gctgtatctg ctgaattaac acgtcaattt ggcagttatg tcgatgttcg
    12421 gacatggggt ggacctactg ccatcttaga gattgtttca aaaggaatcc aaaaagcaaa
    12481 aggcgtgcaa gagattgcta actacctgtc tatcgatcaa aaagacgtca ttgcctttgg
    12541 tgacgagcat aatgatttgg aattgttgga ttatgcaggt tggggagtag ctatggctaa
    12601 cggtactgac caactcaaag gcattgcaaa tgatgtcact cctttgagca atcaggaaga
    12661 cggtttagct gtttatctgg aaaaactact taaactataa tagagaatta gattccacaa
    12721 aaaagaggtt gtgaca
//
LOCUS       DAYYME010000077        12735 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000077 DAYYME010000000
VERSION     DAYYME010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.078"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..816
                     /locus_tag="ACHBFW_001825"
     CDS             4..816
                     /locus_tag="ACHBFW_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFM9318129.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            806..1291
                     /locus_tag="ACHBFW_001826"
     CDS             806..1291
                     /locus_tag="ACHBFW_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9318130.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            1314..1859
                     /locus_tag="ACHBFW_001827"
     CDS             1314..1859
                     /locus_tag="ACHBFW_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFM9318131.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(1932..3575)
                     /locus_tag="ACHBFW_001828"
     CDS             complement(1932..3575)
                     /locus_tag="ACHBFW_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM9318132.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(3704..5932)
                     /locus_tag="ACHBFW_001829"
     CDS             complement(3704..5932)
                     /locus_tag="ACHBFW_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9318133.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(5965..6444)
                     /locus_tag="ACHBFW_001830"
     CDS             complement(5965..6444)
                     /locus_tag="ACHBFW_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFM9318134.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            complement(6675..7523)
                     /locus_tag="ACHBFW_001831"
     CDS             complement(6675..7523)
                     /locus_tag="ACHBFW_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9318135.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            complement(7680..9290)
                     /locus_tag="ACHBFW_001832"
     CDS             complement(7680..9290)
                     /locus_tag="ACHBFW_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM9318136.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            complement(9534..9851)
                     /locus_tag="ACHBFW_001833"
     CDS             complement(9534..9851)
                     /locus_tag="ACHBFW_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM9318137.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            complement(9844..10623)
                     /locus_tag="ACHBFW_001834"
     CDS             complement(9844..10623)
                     /locus_tag="ACHBFW_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM9318138.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            complement(10756..11691)
                     /locus_tag="ACHBFW_001835"
     CDS             complement(10756..11691)
                     /locus_tag="ACHBFW_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM9318139.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            complement(11767..12159)
                     /locus_tag="ACHBFW_001836"
     CDS             complement(11767..12159)
                     /locus_tag="ACHBFW_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFM9318140.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            complement(12140..12658)
                     /locus_tag="ACHBFW_001837"
     CDS             complement(12140..12658)
                     /locus_tag="ACHBFW_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318141.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
BASE COUNT         3744 a         2605 c         2289 g         4097 t
ORIGIN      
        1 gttatgaaaa gaatcttgac aattggcggt tctgatccat ttgctggtgg aggaatccaa
       61 acagatttaa aaacttttga gaattttggt ctattcggac taagcgcctt gacttctata
      121 ggggcactag atattaatga gcgatttatg ctggaatcta tatctttgga attgcttaaa
      181 cgacagttag tttcgattga taaaatgact tcattagatg gtatcaaaat tgggttattg
      241 aattcaagtg aagcaatcat ggttgttcga gacttcataa aaacaaaaca aaatatccca
      301 atcattttag accctgtttt ggcattcaaa gaaactgaag ctgtgatcaa tcaattgtat
      361 atttcgcagc tcatcgaaga actttttcca cttgttacga tgattacgcc taatttggca
      421 gaagctgctt tactttctgg tcaagctctt ccctcttcgt taacagaaat gatccatctc
      481 gctaaagaac tcactgcttc aggaacaaaa aacattgtga tcaaaggcgg cacaaggatg
      541 gctggagatg aagctgtgga tcttctctat tcaattgatc attatgaggt attttctcat
      601 aaaaaaatcg acactacaac agtaaacggg gccggttgtg ccttttcttc tgcaataaca
      661 gcgaatcttg tgttaggggc agattttacc aaagcagtag cagaaagtaa aaaatttgtt
      721 gcccattgta tttctaatgg cgtgctgatg aacgatcata ccggaagtgt ctggtctggc
      781 ggtattcaaa aaggaggaat gagaaatgaa aactaagcaa ttaacgatct atgctatgct
      841 gattgcgttg acagttgccc tttcattaac aattttgatc cctgtaccgg ctacgaatgg
      901 atttgtgaca ttatgcgagg ccgggatcta taccgctgct tttctttatg gtccaacggg
      961 tggattagtt gttggtgctt ccagtggtct attgatcgat ttgatttcag gatatccgca
     1021 gtgggcagtt ttttcattag tgatccacgg gttacaagga tatgttgctg gttctttcag
     1081 caaatcttct actcgtatgt ggggattagg gcttttatta ggaagcttag ttatgatcat
     1141 cggctatggt atcgccggtt ggattttata tgattggccg gctggagtgg cttctatacc
     1201 aggaaacatc ttccaaaatc tgattggaat gggacttgcc tttcctataa ctgcaagtct
     1261 tcaacgtatt caagcacgga agcaatatta attgaaaaag aaggttttag aaaatgaagt
     1321 tgtcagaaaa agaattaaaa gaccaattga ctgaaatagt taatgacata ttggctgagg
     1381 cacacttgaa aaaaggtgat ctttttgttt taggatgcag cacaagcgaa gtcgttggag
     1441 gacatattgg aaaaaattcc agtgcggaag ttggtcaatg gatcatccgt acgttaaaag
     1501 agttgttgga tcctaaagaa attgcgctag ctgtccaagg ttgtgaacat ttgaaccgag
     1561 cattagtagt agaacgaaca gtggcagaag caaaaaattt cgaaattgtc tctgttgttc
     1621 ctgcactaca tgctggaggt gcttgttctg ttgctgcttt tgaccagttc aatgatcctg
     1681 tcgaagtgga acacgtagtt ggacaagccg gtatcgatat cggtgacaca tcaatcggta
     1741 tgcatatcaa acatgtccaa gtacctgtca gacctcgttt gaaaaccctt ggacaagctc
     1801 acgtgacagc ccttcgctct cgtcctaaat atatcggtgg tcctagagcg aattattgaa
     1861 atgagtaatt gacagagcaa tctgaaaaac ttcctcgcaa agtccaacta atgaacttta
     1921 acgaggaagt tttacttgtt tgttttatat aaccgagctt cataaggtgc tagtatgcct
     1981 ttagcttcaa attctccgct agaggaattt gacaagagta atgtccagga ctgatctgct
     2041 tcttttggaa gttcatagtc agcaagtgat tctgttaagt ttaccataat aagatattgt
     2101 tcatcttcta aatatctttc atagacataa agttgtggat gttcatgaag atattcttta
     2161 taagaaccgt atatcagacc tttattggtt tgtctaatgc ggatcatgtt cttatagaat
     2221 tgcaggaccg actctggttc atattcttgt tgttctacat tgatcgtttt ggtatttgga
     2281 ttagtgatca gccaaggttc atgtgtagaa aaaccagcat attgttccga agtccattgc
     2341 atgggtgtcc tgctgttgtc tctcgttgtt tcacgaatga tatccaatgc ttcttctctt
     2401 gtcttacctt ccgcaagcag ccttttataa agtcttttcg aatctactgc atcaacttga
     2461 tcgatcgatt caaagggcat attcgtcatt ccgatttcct gtccttgata aataaatggc
     2521 gtaccttgca aaaagaaata catcatagcg attgcttttg cagacatagc ccaaaaatcg
     2581 ggacgagtat ctccaaacac tgaaacggca cgaggaacat catggttttc catatataaa
     2641 gcgttccatc cgataccatc caatgaagtt tgccaagcgc ttaatgcaag tttcaattta
     2701 aggacatcta attgcccttc tttttcttgc tgccagatat ggatatgatc aaattcgaat
     2761 atcatatcga aataaccgtc tttccctacc cattgtggtc cttcttctgc agtgacacca
     2821 cttgcttctc cgacagtcat gatatcgtac tcttcaaaaa catgtttcaa atcggtcaaa
     2881 tgttcttcga ttcctgagac attttgaaag ggtgagaaag ggttctctgt ggcttttact
     2941 gaatattcat ctttttttac atgagaaatc gcatctaaac gaaatccatc tatcccttta
     3001 tccaaccacc atcggatcat attgaatagt tcttctttca atttctcgct ttcccaattt
     3061 aagtctggtt gttcttttgc aaaaacatgc agataatact gtccagtttc atgagaatac
     3121 tcccaagcag aaccgccgaa aatcgatgtc caattattcg gttctttgcc atctaccccg
     3181 tcgacccaaa tgtaataatc gcgatacggg ctttctttag attttttcga ttcttcaaac
     3241 cattgatgct gatccgaact atggttgatg acaagatcca taatcacttt gatccctaat
     3301 tcatgtgctt cttttagcaa taaatcaaaa tcttccatcg ttccgaatct ctcttctata
     3361 tcttgatagt cacttacgtc ataaccatta tcgacaaatg gagattgata gatcggattg
     3421 atccagataa agccgatacc aagatctttc aaataattaa gtttttcacg gatcccattg
     3481 atgtctcctt gaccatcgcc attgctatct ttgaaactag caggatagat ttgatagcct
     3541 actgcttctt tccaccattt ttgttcttgt atcatatcgt ttcactcctt taacggtccc
     3601 tcttgattat aaccgattct attttagaag tatccttgtt accggtaact agaaacgaaa
     3661 agctcaacga atctgctaag tgcgttcgtt gagctttatt ttatcataga tagcgggcaa
     3721 tcagttgaat catttgcttg ggaaattcat tcaagtcagt aatatctaca aaacgacttt
     3781 caccgtagat cgacttgatt tcttctttgt cttggccaat agctgcggag acaaatagga
     3841 ttccttgacg ttcatattct ttcaaaacag actggatatc ttcttttgct tttttgcctg
     3901 tataatccgg caacgctttt ggctgaccgt cactaatatt aagcaacagt ttggttgttg
     3961 cggattgtct gtttaatttt tctgctgcta aacgaagtgc agcaccatca cgattgtttt
     4021 gccttggttt catagtgact aatttttcat ttaaccattg aaatccgtct tcaaattctt
     4081 tataagaaaa caaagaagtc ttttctcttg acgaacggtc tgctgtatct ccgtaaatca
     4141 acaatgggat atcgactttc ttagaaaatt cggcaatggc aatcgctgct ttttttgccg
     4201 cttcgatccg atcatcccgt atcattgacc cagattcatc gatccgtaca gctaccgcta
     4261 aagaaggttg ctcatgtggc gggttctttt tgtcaaaatt tctcaaatca ccatatgcta
     4321 ctttgcttgc attgaaccgc tgaccttcgt attttccttt ttggtacgtc tctgtttgtt
     4381 cattatccag taactctaaa atttgccggc taagtgattc tactattggc attacttgca
     4441 tgtgtagctc acgtgcttcc ggcgtttttc cttggaaagc tggtcggtga acgatgagac
     4501 cttctttttc atgaatcgtt tccttcatga tttgtcgcgc ttctttattc agttgcttct
     4561 tgatttcctt atcatgtgct tccatgtcag aagcagacat ttcttgagaa tcatctcctg
     4621 tttgttgttc tttttgattt tttccttcga tatcgctact gcgttctttt tgcatttgag
     4681 gaggaatctc gtttttttct attgtttctt cttgtttctc ttctccctca tttggttctt
     4741 cagcagacaa cgatttcttt tcatctaagc gcagaatttc ttcagcattt tcagtgatgt
     4801 ttcgttcatt tgcttcttcg gcgctaatgg ttcttccttt tgtatgctca gattgaggat
     4861 gaccccattt ttcttttctc aatatgtcct gaagttccag tagcaatcca ctgtcagaca
     4921 aagcttgttc aagaattgcc aattcatctg aatcagtgga taatttatac aaaacttttg
     4981 gataaatcga ctggagtaaa tcttgtcctt gcttataggt atttgcccaa taaaaataag
     5041 aacgcattcc tgctacacct ttgatggcgt tagcttttgc agtttcatcc aacaaacgga
     5101 taatctctgc catctttaag agaagttctt gtttttggat catagttttc gccatcgcac
     5161 gttctgccat gacttctaag gcaggaagat ccattttttc cgcatgttgc atccgatctc
     5221 ttaaagattc atttaatggg cgatttcctt ggtaatttcg atttgtagtg atcacaatga
     5281 cgcaatctgg atggcgacga atgattcctg tcggtaaatt caacatccca tttggttcga
     5341 gcgctgagtt aagtgccaca agaacagagg catctcggat gacagtcggc tcttgaatct
     5401 ctaataaata tcctttttca atcgctcgaa cgatttcaga agggtaatag ctgtaatgga
     5461 ttgcatctgg ataatcagct tcgatccttt gaactagctc tgctaatttt tctttagcag
     5521 aaaaaggatc aatgccgtaa tggttctgga tcagtgtcag tacagcttca agtgtatctg
     5581 tttggtaaat agcttctagt aattcttggt cttgttcctt attagaatct actacaggta
     5641 ataaggctcc gaaaacatct gatttgtcca tatctgcaaa acaggtcact tttgtatagg
     5701 gaagttgcag atcagcagat agtgctttgg ctaattgtgt cttccccgac ccggcatctc
     5761 cttccaaaag tacgttactg attttcattt caggatcttg ccaattccct ttgatttcgg
     5821 aggcgatccg ccattcttct gcacttgtcc gatggctttt cggtttcttc cagatcattt
     5881 gcttttctag ccgtgaaaag gttcggtttt ctgttagtga gaaattctcc atgcttctct
     5941 tccccttttg atcatctctg tctattaata tcctagctca tctaaaagac gttttaaaat
     6001 acggaatcgt tccccaatca gttctccgtc tttttgtagt gtatcgtaat acaaaatgat
     6061 gtcttcatcg atctttggat ttttggtatc tacttctacc gtcattcggt tcccttgtaa
     6121 tccaatgatg tctacgactg gattttctgg atcataaaat ttttcataac gataagcatc
     6181 ttggaagtat aaacttcctt gtgcagctaa gatcgctaaa tctaaaatac gtgtgattgg
     6241 taattcttcc gactgtcgtg accatttttc tccagtatgt cgccatacct tgcctgagat
     6301 atcgactttt ccacgatcat tccactgagc aaggcccagt gaaagccctt ttgcatccgt
     6361 atttaatgca tttcttccat caactttatc atagtcttct acaacgatta ctggtttatg
     6421 ttttaaattt gttggtattt ccattatttt aactcctcat ccttttattt ttagtaaata
     6481 agtaaattag tagtttactc atttactaaa ctatatgcga cattatagag gattgaagtg
     6541 aggattgcaa agaaaaagag cttcattgat agaaataaaa acaccatctc gttcttttac
     6601 acgctcatta gtaacatgta gaaagtccaa gatgatgttg gaatattttt ggtatgtttt
     6661 ttgaataaaa ggaattattg cgctgaaatg gcaatgccga tggctttttc acctaaatgt
     6721 gtaccaatga ctggtccgaa atgaccaatt tcgatttctg catttggata ttttttctgc
     6781 aatttctctt tctccgcaat cgctacttct aaattatttg catgtatcac gtaaagtttg
     6841 actggcggtt gtatttcgtt atttcttcta ccaataattt cttcagctcg agcaaatgct
     6901 tttttacttg aacggatttt ttcgaataaa acaattttcc cttcagaaaa tgtcaggatc
     6961 ggtttgattt ttaataagct gccaatcagc gctgcaccat ttgtcaaacg tcctcctcgt
     7021 actaaattgt tcaaatcatc tacaatcaga taagcgtagg tattatctcg gattcgatcg
     7081 atatgtgcaa gtatttcatc aagggactga ccttcatcat ttaagtctaa ggcagcttct
     7141 accatgtgtc ccatcggcat actagtgatc ttagagtcat aaggaatgac cgttactccg
     7201 ctgatgtcat ctttcatcgc aaataatgtg ttgacaaagc cagaaatccc tgaagaaaga
     7261 tggatactta aaatcgtgtc ataccctttc tctttcaatt cctcatataa acccaaaact
     7321 tcccctacga caggttgtga agtagtagga aaatctttac tgttttttaa aagtccataa
     7381 tattcatccg cttcgatatc gattccttcg ttataaattt tcccatccat aatcaaaggg
     7441 attggaataa cgaataagtc gggatggttc ttgatacgtt ccggcagata tgccgaacta
     7501 tctgtaacta ctgcaagttt catgtgttta atttcctcgt ttcaaagaat ttttccagac
     7561 cttataacta tatcataagt aagattagat atgaaactaa aattggaaaa tagaaaaacg
     7621 acaagcgtaa tcatacgagt tgccgttttt caagtgacag caaaaaacga ttagttaacc
     7681 tattctttgc ttctgctatg atgtttaaca tttttcaaca ttcgaaaccc attttttccg
     7741 aattgagaaa ataaaaatac taagccgcca atacttccta cagccacaca ataaatcatt
     7801 gcatagcttt ttgattccat tgacaatcgc tgattcagca aaaagaaaac ggcaaaacat
     7861 actgcaagca taatcagggt ggagcggaaa gtttttaaga aaaaattgct gatctttgct
     7921 aaaggtgcta ttcggtattc tttgcacata aagtgatagc cgcgaacaaa gacaataccg
     7981 aatgctaagc catttgataa gctcatcccg tatccgccaa ctaatcctaa tccgattact
     8041 tggaagatga ctttcaggat aattgcttct acagttaatc ggatcgcaaa acgatggtga
     8101 ttcaaagatt gaagcatcgt agataggatc gtgaataaag agaaaaacaa agaagctagc
     8161 aaagccattt ggaaatatcc gacactttca ggatcatagc caaaaaacaa tgtgtatagt
     8221 ggtccagcta ataaagacat tccagtcaat gaaggaagaa gaatcaataa cgcaatcgaa
     8281 aaactatctc ctactgtttt ttcgatttgt atccgatctt ttttcttcat cgtgcttaat
     8341 attggcaagc tgcttatagc tactgttcct accatggaga tcaggatcaa tgtcaattta
     8401 tttggattga cagaagcacg gctgaataag aattccagtt gctgatctgc gatctctgga
     8461 cggaaaaagt gaagcagtgg tttcatcgtt acttggtcga tcatttgaac gatagtaatg
     8521 actgagccga caaaaataaa cggtagtgtt tcccgaatga tagagacaga ggcagatcgg
     8581 ttttctcttt cgaaataacg gctggaaatc aaaaaatctt taagttcgaa gaagtctttt
     8641 ctgcgtccga cgatatacat atggataatc gcagctaacc caccgaagaa agaagcgact
     8701 gtactgatca atacagcttc taatatggtt ccgtcagtca atacgcgcaa gtaataagta
     8761 ccagccaaga taacgatcac tcgcacagcc tgttcgataa tcaaagaagt tccataaggc
     8821 cgcagatcat tttttccttg gaaatatcct ctcattgcac taagaattgg gatagcaatc
     8881 aaagatgggc ataaacttct gatagctagt atcccattat taacgttagc aatcggactg
     8941 attccggcta gtaaaggagc aaataaatac atcagtgcag cagaggcgat accgatgaca
     9001 aacatgatat tgatcgtact tctaaaaatc agccgacaag cagcgttatc tccttctttt
     9061 gtggctacag ctactttttt tgcaatcgca tttggaaatc cgactgtacc aagcatcagg
     9121 aacaatccat aaggaagata gccaacatta tacaatgtcg tagcaagcat accatcttga
     9181 ttgttcccca tcatacttag ccagggaatc aaatagacta cccctaaaac tttacataga
     9241 agattagcaa aggttagcca aaatgttcct tgcatcaatt ttttattcat ttaaattccc
     9301 tcaactattc tttcataaaa taaacatctt ccctattata gcaaaaaatc atagaaggac
     9361 aaatccctct ctaattccta aaataaaaaa gagcttgcat cataagccaa cactaatctt
     9421 ttttatccct acgaataaac ttgcatccag aattgactgt tgcaaatgca gttcggatag
     9481 atcagtttga acaacttatg tcgcaatctc tgtttttaat aaaataaaac agtttaaagc
     9541 atagacagca aaaagacaga cgcaagtcct gtcaaaactg acaacgccat cgaacgtgtt
     9601 ttgtaagcca cgcctgctac tagaatcatt gcagctattt tgatattcca agcaatatcc
     9661 agatgttcat gagtaatgaa aacatcttta aaagctaacg ccgcaaataa tgccaccggt
     9721 acatatttca tccagtcact gaaccaggat ggcacttttc gatgagtaaa ataaagcatc
     9781 gggaacaagc gcgggatata tgccacgatg aacagacaag caactaaaag tagcaaatat
     9841 agtttagtca tggtgattgg cctcctgttc cgggaagttg aagagcggct cattgacatc
     9901 tttaccgcgg tctcttagtt tcttagcttt ttcttttttg aatgccttct ccagcataaa
     9961 tccggcaaat gaagcaatga ctgttgctgc aatcaatccg aaagtatttt ggaataatac
    10021 catgaatacg acccctaata cgcctgagaa taaacctgta aagatcagta gtgcgctttt
    10081 catctgcatg ataaacatga aaatgaacat ggccgtcaaa gcaaaatgga caaggtcagc
    10141 atctactcga atcaccgagc cgaaaaaatt accgatcata ttgctaccgg cccatgcagt
    10201 cattgagaac caattgacat ataaagcctt tcttttattc caggaagggt cagttgaaaa
    10261 ctttaagtag ttcaccgcat agttttcatc attcagtgat tgagaaaaga cagccaaaaa
    10321 cgtccgcttt tcttttttca taaaaccggc aagacttgct cctaataatg tatagcgtaa
    10381 ttctaaaaag aaaaccatca ataatgtcgt tgcaaatgat gcttgcgtcg taagcataga
    10441 tgcaactaaa aactgcgcgc caccggaaaa gaccagaatc gataacagtc ctgataagag
    10501 aggatcaaat ccaacttttt gcaacaatac tccgcaagct aatcctactg gtatatagct
    10561 tatacaaagt ggcatcactg catgcaaagc ttgaagccat ggcgcacttt tctttttgtt
    10621 cattaaaata tctcctttaa ttacaacaaa taaacagcgg actcattgta acataaattc
    10681 gctaaattgt agccgtttta tttaaaaaca agattatatt tttctcattt ttgcttcaat
    10741 tcatgaataa acgttttatt tacgagaata atttgggtca tatttatctg tttcaatgta
    10801 tatatacatc ttcttgtcag gaagagactg acgcatattt ttttcaatat cattgatgac
    10861 cgagtaagct tcattctctt tgttaccaac gatatctgct tttgctgcaa ctaaaatctc
    10921 cgttggactt aaatgaactg tttttatatc gatcagccga ctgatctcag gacgttcaaa
    10981 agctaacttg atttttgata aatcgcttgc tgtcacgctt tcaccgatga tcagactata
    11041 aaattctttt gctaggaaaa tggcggctcc catcagcaag aaaccgatca gcaatccgct
    11101 gatcgcatca aatgcagcaa tcccggttgc ataagtcaat attgttcctg ctaaagccaa
    11161 gaccaaaccg attacagcac acaagtcttc cgtaaaaata atcagtattt cactgtgtcg
    11221 actttcgcgc aaaaagcgca tcgtggaaag tttttcagta ttcaattctt tgatttcttt
    11281 cattgccacg cgtaacgaac ttccttcgat taccaaacca aataacagta ttgctataac
    11341 aatgatcgga ttacccactt catgggatgg atgcgtcaac ttttcaaaag cttccatcac
    11401 cccaagtgcc ccaccgccga agaaaagcat cattgcgacg attgtactga aaaagtattt
    11461 tgctcgtcct tcaccaaatt gatgcaactc gctctgtcct ttattggctt gtttatcacc
    11521 gaataacagc aaaatctgat tgctgcaatc aacgatactg tggatgcttt cgtttagcat
    11581 ggcggcactg ccactgattg catagccgac aaatttgctg atagcaacta gaatattagc
    11641 ccctagagca gcaattacgg aaaacattcc gcttttttta aaattttcca tgttattttc
    11701 cttctttcat caactgaact cactgaatta tggcaatatt cgccctttaa cagtttttta
    11761 tgaaacttat agtttagaca aattgaaaat gaccgattgt ccatcattca atttgatctc
    11821 tttggcacca gtagtagcac ttttcccgtc cacagtaaac atccaataga gttgattttt
    11881 ctcatcctgt gagtgccctt caagcgaagt gatgaagccg ttatcttctt tgacttcaaa
    11941 atttcgtttc aaaatactca gtaaatcatc gccttttttg aagaacactt ctttttccga
    12001 aaagttttga ccattttctt gtaacgtgat tgttgctttt tgcgatgttt ttacttcctc
    12061 cttttggttg gaagccgatt gtgtataggt tgtactgcaa cttgtcaaaa tgagtatact
    12121 gacactcatt gcaagcaaac tataaattat ttttttcatt ttttcctcct gaatgattgt
    12181 aaaaacgttt gacgactggc agaaaaacaa gaaagaacac aacattgcct atactgtgca
    12241 tcaaatcaaa cgacacccct tgaaggtaat agatccaagg ttgaggcaaa cgatataaca
    12301 gcgtatcaaa aacggtcata cctattccgt aaaccatgcc cgccagaaaa aaacagactc
    12361 cctttaacca tagattcctt tgcactgttt ttctcaagca gacgattgaa aacaaactca
    12421 gaacaacagt atagctgatc cattggccga tgacccatgg tccaacacca aaataaaaac
    12481 tgctgatcgc aatagaaaga ctcatgacaa ttaacgcatc ttttaagctt aagtaaagta
    12541 ctataaataa aaaaatagca gtcattggct ggaaattcgg aataaattga aaagcgatcc
    12601 tacctgctac acaagcagca gacaataagc taagctgtgc tattttattt gtggacattc
    12661 ctaatgaaat ccctccttca tcagcaagtt attcacgtcc atttagtaca tacagaaaag
    12721 acttgtgaca attat
//
LOCUS       DAYYME010000078        12705 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000078 DAYYME010000000
VERSION     DAYYME010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.079"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            211..1056
                     /locus_tag="ACHBFW_001838"
     CDS             211..1056
                     /locus_tag="ACHBFW_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9318142.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            1247..2434
                     /locus_tag="ACHBFW_001839"
     CDS             1247..2434
                     /locus_tag="ACHBFW_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM9318143.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            2440..2796
                     /locus_tag="ACHBFW_001840"
     CDS             2440..2796
                     /locus_tag="ACHBFW_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFM9318144.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            2798..3136
                     /locus_tag="ACHBFW_001841"
     CDS             2798..3136
                     /locus_tag="ACHBFW_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFM9318145.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    3212..3468
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3531..4565
                     /locus_tag="ACHBFW_001842"
     CDS             3531..4565
                     /locus_tag="ACHBFW_001842"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM9318146.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            4558..5244
                     /locus_tag="ACHBFW_001843"
     CDS             4558..5244
                     /locus_tag="ACHBFW_001843"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM9318147.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            5268..6116
                     /locus_tag="ACHBFW_001844"
     CDS             5268..6116
                     /locus_tag="ACHBFW_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM9318148.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEEKPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            6313..7086
                     /gene="sufC"
                     /locus_tag="ACHBFW_001845"
     CDS             6313..7086
                     /gene="sufC"
                     /locus_tag="ACHBFW_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFM9318149.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            7099..8385
                     /gene="sufD"
                     /locus_tag="ACHBFW_001846"
     CDS             7099..8385
                     /gene="sufD"
                     /locus_tag="ACHBFW_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFM9318150.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            8382..9617
                     /locus_tag="ACHBFW_001847"
     CDS             8382..9617
                     /locus_tag="ACHBFW_001847"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFM9318151.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            9604..10074
                     /gene="sufU"
                     /locus_tag="ACHBFW_001848"
     CDS             9604..10074
                     /gene="sufU"
                     /locus_tag="ACHBFW_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFM9318152.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            10079..11470
                     /gene="sufB"
                     /locus_tag="ACHBFW_001849"
     CDS             10079..11470
                     /gene="sufB"
                     /locus_tag="ACHBFW_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFM9318153.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(11568..>12705)
                     /locus_tag="ACHBFW_001850"
     CDS             complement(11568..>12705)
                     /locus_tag="ACHBFW_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9318154.1"
                     /translation="TFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAEAQ
                     TALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILPAF
                     GELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTLLP
                     RKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWEDID
                     YFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMILY
                     GYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLFES
                     GASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
BASE COUNT         4081 a         2370 c         2776 g         3478 t
ORIGIN      
        1 aatagatttt agtaccaaga tgaacgaata cattgtaaaa tgaataggaa tgtatgatta
       61 cgagcaagca gacagctgat tgtcttcatc taagttacta gttggatcag caactgtttt
      121 gttgtacatt tctattatag atggtttttt gtatatttaa atcaaatagt ttttatagta
      181 attatcataa aaagttatgg aagtggtgcc atgtttaagt tattaacgac ttttcgagca
      241 gtatatgaaa caagaaattt ttccagagca gcagaagtac tgtttttatc tcaaccagca
      301 gtctcgaatc aaatcaaaca gttggagcgg gaattgaata tcgatctttt tcaacgtaac
      361 ggaagaaaag agatgcttcc aactaagcaa gccgacttat tatatgaacg gacactggtt
      421 ttgttagaag aatgggaaga aactcagcaa aaattgctca atgaacaaaa tgaagaagaa
      481 gtatgtcgga tcgttgcttc ccacaccttt gctgtctatc ttttgcctca gttgatgaaa
      541 catctgatca aaaggtttcc taaagtgcat tttgagatcg aaatcacgaa ttctcatgaa
      601 tcattagaac aagtagcgaa acatgaatcc gattttggtt ttatcgaaaa gccattagag
      661 acttccggcg tacaaagata ctctttgatg gaagaccagc tagtcatcgc tggggaccca
      721 aaaagcaaat tgtggcttgt aagagaagca aactctggtg tttttcatta cacgcagcgt
      781 tattttgaag agatgaatat ccaagataag aagatgatcg tgaaaagtaa cgatgtaatt
      841 gttgcgttgc tgaaggaagg aatcggtcgc tcgattttgt ctaagcgctc tgttccagaa
      901 aatgtgccaa tgactccgtt gaatttccat cgatattttt atttcatcca acgaaccaat
      961 ctggtttctg atcagctgct agagattgcc gaagagatcc ttcgatacaa tgaaagcaca
     1021 gaaacttctc acagcgagga gaatctcagc tggtagataa ttgcatgtat caaaaaaagt
     1081 tctatgatac actaaagata agcagtaggc ttttgtactg catttgctca agcaactgca
     1141 caaacggaaa gttaattttt cccaaatgtc tatggaaaga ctttccatta tctaactggg
     1201 ccgctataat aatcagcagt atggttttta agaaaaggga tgaaacatgg aaaaaaaatt
     1261 aaaagcttcg aacgataatc gtattgatta tggcgtgatt ttaccagtct ttctgttatg
     1321 tttgattggt ctgctctcat tatatgtagc acttagccat gatccgaaca atccagcagt
     1381 cttgaaaggc gtagggatgc aagttgtctg gtatttagtt ggagcggcag caatcgttgt
     1441 catcatgcat ttgaattcca aatggatctg gaaactgact ccttaccttt atggtttggg
     1501 gttagtggta atgggactct tgctcaaatt ctatgatcag aatttagctg cttcaacagg
     1561 gtctaaaaac tggtttcgat tcggtgcgtt tactttccag ccagcagagt tgatgaagat
     1621 tgcgtacatc ttgatgatgg cactgatcgt aactaaacat aatacgcaag taaaagaacg
     1681 gacgatgaaa tcggactttt ggctgattgg gaagctcttg atcgtgacaa taccggtttt
     1741 agcattgatc atggcccaag atgactttgg aacgatgctt gtctttcttg cgatctttgg
     1801 cgggattttc ctgatgtctg gaatctcttg gcgcatcatc gcacctgtag taatcttggc
     1861 agtagtcgtt ggagcaggaa cgatcttctt ggttacgact gaaggcggaa gagacttgct
     1921 ttataaagtt ggattcaaat cttatcagtt tgcacgtatt gattcatggc tggacccttt
     1981 ccatgatacg tcaggaatga gttatcagcc ggcccaagga cttttggcaa ttggtaccgg
     2041 tggcttgttt ggtaaaggat tcaacgtaag caatatctat gtgcctgttc gtgagtccga
     2101 tatgattttt accgttatcg gtgaaaactt cggctttatc ggtggtgcat tcgtcatttt
     2161 cttgtatttt attttaatct atcgtatgat tcgggtgtgt ttcgatacga ataatgaatt
     2221 ttacgcctat atcgcaagcg gtttgatcat gatgctgctg ttccatgtct ttgagaatat
     2281 tggagcaaat atcggattac ttcctttaac agggatccca ttgcctttca ttagtcaggg
     2341 aggatcctcg attttaggga acatgatcgg tatcgggttg atattatcga tgagatacca
     2401 aaatgaagca cctgcaagat cttcaaggag gtaagaaaat tgtacacctt ttactggtac
     2461 ccgaaatgtt caacatgcaa aaaagccaaa gcgtggttag accagcatgg cgttagttat
     2521 caaaccgttg atatgatcaa agaagtaccg aaggcctcaa cgattgagac ttggctagaa
     2581 acgagtcaag taccaatccg tcgcctgttc aacacaagcg ggatgaaata tcgggaactt
     2641 ggactgaaag atcaagtagg cgagtttacg gcttcaaaag cgagccaagt cttatcgaca
     2701 gatggtatgc tgatcaagcg accactgatc ttacgagagg accagcttgt tgcaattgga
     2761 ttcaatgaaa aaatttatga aggagaactg atataaaatg gcaggcaagc atttgaagaa
     2821 aaaggacaac ctttggatct tatttaatgg aaaagaatac tgtgttggtt tgacaaatga
     2881 agcgcaagaa gaacttggag acatcacgtt tgccaatctg ccaaaagcag gacaatcgct
     2941 aaaaaaagat gaacctctga tcgaagttga agcggaaaaa gcggtcaatg aatttgccag
     3001 cccgctaacg ggtacagttt cttctgttaa tgataaaatc ggtgaagata tcaatatctt
     3061 gaatgattca gatgaaatga atgcgtggat cttaagtttc aaagatgttg atccaaaaga
     3121 atttgacgaa ttataaaatc agaaaatcac ttgaaaaaag ttgacactga ttcttccagt
     3181 tgctataatc ataagcaatc ctatatgaaa cgagaaagct ttgaaaagaa gagtacatgt
     3241 cacggacgtt tgaagagagc cctgtttgct gaaaatgggt aacgaaaatg agatggaaga
     3301 tggtcttaga gcagagtaag cgagcgtaag caaacttact acgggagtgc ccgttacagc
     3361 gcgacagtat acggaaagaa gctaagtcca agtactggaa gaggctgtta ttgcgaagta
     3421 acggtaaatc aaggtggtaa cacggaagtt caagctttcg tccttttgct taaggcaaga
     3481 gggcgaaggc ttttttgttt gaaaaattat aagagaaaga tgtgaatggc atgtcattaa
     3541 tccaactaac gaatgtcaaa aagacattca atgggaaaaa cggaaacatc caagccgtca
     3601 atgacgtttc actaaacgtt gaaaaagggg atatttatgg aatcgtcggc tattctggcg
     3661 caggaaaaag tacattagtc cgtctcctga atggacttga gcttcccacg agcggcgaag
     3721 tgatcgtaaa cgatcaagat attacaaaat taaaaaacaa agaattacgt acattccgaa
     3781 agaaaatcgg tatgatcttc cagcatttca acctgttatg gtcaagaact gtacttgaaa
     3841 atatccagct gccgctggaa ttagctggcg taccaaagag caaacgtaaa gaacgggcag
     3901 aagaactgtt gcgtttagtc gggttagaag gacgcggaca agcatatcct tcacaacttt
     3961 ccggcggaca gaaacaacgc gtcggtatcg cacgagcatt ggcaaatgat cctgagatcc
     4021 tgctttgtga tgaggctact agcgcattgg acccgcaaac aacagaagaa gtattagact
     4081 tattgttagc gatcaataag aaattgaatt tgaccatcgt cttgatcaca catgaaatga
     4141 atgtgatccg caaaatctgc aacaaagtcg ctgtgatgga gttggggaaa gtcgtagaag
     4201 aaggagacgt attgactgtc ttcagacaac cgaaaaaaga agtaaccaaa cgctttgtac
     4261 aacaagacat cgaaccagaa gctgattcga cagaactatt agctgaactc atcaaagaaa
     4321 atccaagcgg ccgtttggca actttgacct ttagcgaaag aaatgccaac gagccagtca
     4381 tttcccaagc gattcgacgc tataacatcg atgtcaacgt tgtgtatgga aagatcaaac
     4441 aagtgaaaga aggttccttt ggttcattga ctgtcttgat gactggtgaa accaaagaat
     4501 tagatcaagc agaagtgttc atcaaagaac aaggagtagg aattgaggtg atccatcgtg
     4561 gataaaagct tttcagaaac atatctagat tttaaacaaa tcaacccgga aacgatgaat
     4621 caggctatcc ttgatacact atatatgaca ttaatctcaa tggtcttagt gacgatcatc
     4681 ggattagttt tgggattgat cctttactct attggacgca aaaacaaacc gtatgctcga
     4741 atcatttatg gaatcgtatc gatcatcagt aatattttcc ggtcaacgcc atttatgatc
     4801 ctgatggttt tgatcattcc atttactaaa gcaatcgtag gcactatgct aggtgcgcaa
     4861 gcagccattc cagctcttgt tttatccgca gcaccatttt atgcccgtct agtagagatt
     4921 gcttttcgtg aagtcagccc tggtgtactg gaagcagcag atgcaatggg agcaagttac
     4981 tgggaaatca tctggaaggt cttgatacct gaaagtattc ccgcattgat ttctggatta
     5041 acagttacaa cgatctcgat gatcggattt actgcgatgg ccggcgcgat cggcgcaggt
     5101 ggacttggag gtcttgcttg gcaggaaggc taccagcgtg gtaatctaac agtgacattc
     5161 gttgcgactt taattatttt actaatcgta tttatcgttc aaggaatcgg tgatttctta
     5221 actaagaaaa cagacaaaag ataataggat atagggggat gtaggatatg aaaaagaaaa
     5281 tttttggatt tgcagcagcg cttttgctga ctgtaggatt agcagcatgc ggtaacaaca
     5341 gcgacagcaa agattcagca aataaagcag acgacacgac attgaaagtc ggcgcttcac
     5401 cgacaccgca cgcagagatt ttggaacatg tgaaaccatt attgaaagat gaaggaatcg
     5461 atcttgaaat cgtcaaattc gatgactatg tattaccgaa ccaatctttg gaagaaggcg
     5521 acattgatgc caattacttc cagcatattc cttatttgaa caaagaaatc aaagaaaaag
     5581 gatatgactt tgtcaatgcc ggtgctgtcc acatcgaacc aatgggcttg tactctaaaa
     5641 aagtcaaaga tgtttccgaa ttaaaagatg gtgcaacaat cattacttca aattctgaat
     5701 cagactgggg acgcatcatc acaattctac aagatgctgg cttagtcaaa gtcaaagatg
     5761 gagtagatct ggaaacagca acattcgatg atattgaaga aaaacctaag aacttgaagt
     5821 tcaaccatac gatcgaccca tcacttttag cttctacgta ccaaaatgat gaaggtgacc
     5881 tagttgcaat caacgcaaac ttcgcttacg gcgcagggtt gaatccatta gaagatgccg
     5941 tattgcttga aaaggacaac tctccttacg tgaatattat tgcgactcgt aaaggtgatg
     6001 aagacagtga caaaatcaaa aaactgattg aagtgcttca ccgcaaagaa gtccaagaat
     6061 ggatcacaga caaatggggc ggctctgtta aaccagtaaa ggctgatgca aaataaggta
     6121 tggaacaaaa aagatgagaa ataagtctgt aatggcttat ttctcatctt ttttatacct
     6181 attctaattt agaaatcaaa attgtttaat aataaataaa agatatttga ttttagtaag
     6241 gaaggtcatt gcgcttcatt ctcaattagt ctaaaatgag tgagaacgaa agagaatttg
     6301 ggggtataat gcatgtctgt cttagaaata aaaaatctac atgtgtcgat cgaggaaaaa
     6361 gaaatcctta aaggcgtaaa cctaacaatg aaaaccggag aaatccatgc catcatggga
     6421 cctaacggca caggaaaatc taccttatct gccgctatca tgggcaatcc aaactatgaa
     6481 gtaacagaag gcgaaatttt gtttgacggg aaaaatgttt tagacttgga agtagatgaa
     6541 cgtgcgcgac ttggcttatt tttagctatg caatatccaa gtgagatacc aggtatcacg
     6601 aatgcggaat tcatgcgtgc agcgatcaat gcgaaacgtg atgaaaacga caaaatttct
     6661 gttatggcat ttttgaaaaa gttagatgaa aaaatggctt tactaaatat gccagaagaa
     6721 atggctgaac gttatctgaa cgaaggtttt tctggcggag aaaagaaacg gaatgagatc
     6781 ctgcaattgt tgatgttaga acctacattt gcaattttag acgaaatcga ttcaggtctg
     6841 gatatcgatg ctttgaaagt cgttgctaaa ggagtaaatg aaatgcgcgg agacaatttc
     6901 ggtgcattga tcatcaccca ctatcaacga ttattgaact atatcacacc tgatgtcgtt
     6961 cacatcatga tggaaggccg agtagtcatg acagggaatg ctgacctagc taaacgtctg
     7021 gaagcagaag gctacgctgg tatcagtaaa gaattaggaa tcgactataa agaagaagaa
     7081 gcgtaagggg gagcaagaat gaaagaaaaa aactggctcg attatcttga cgcggtaaat
     7141 gatttttctt taagtaaagg cgaacctgat tggatgagaa ccttccgtca agacgcacta
     7201 gcgaaagcag atgaactgcc attgccgcat atcgatcggg tgaaattcca tcgctggtcg
     7261 ttatttgatg tgaaagaaac acaaaccatc agtgaaacag gcacgattcc ggcatttgat
     7321 gcaatgaaag acaatcctgt gcttgtccaa caaggttcat ggacgatttt tgaacaactg
     7381 cctgtggaat tagcagaaaa aggagtcatt tttaccgact tgttcactgc aatgatcgaa
     7441 tatccagaac tagtccaaga atattatatg aaaaaagcag taaacatgaa cgaagaccaa
     7501 ttgactgcac tccatgtagc ttttatgaac agcggtattt tcctgtatgt gcctaaaaat
     7561 gtcgtgatcg atgaaccact cgagtcctta ttcattcaag atggcgcatc cgatgagcac
     7621 ttcttcaagc atgtgttgat cgtggcagat gaacatagtg aattttctta tctggaacgt
     7681 ttccagacaa ccaaagaaca agtagcaaaa tcatcaggta atatcatcgt ggaagtcatc
     7741 gctaaagctg gttcaaaaat caaatattca gccgtagatc aattaggaga aaatattact
     7801 tcttatatga atcgccgtgg gcacatttta cgagatgctt ctgtcgactg ggcaattggt
     7861 gtcatgaatg acggtcatgt gatcgctgac tttgactcag atttagcagg agaaggtgca
     7921 cacgcagaag tcaaaatcgt ggcaatcagc tcgggcagac aaatccaagg aatcgataca
     7981 cgagtaacca ataaagcccc tcatacgatc gggcatattt tgcaacacgg agtgatccga
     8041 gaaaaaggaa cattgacctt taatggtatc ggccacatct tgaaaggggc aaaaggtgca
     8101 gatgcccaac aagaaagtcg tgtcttgatg ctttctgata aagcacgtgg tgatgcgaat
     8161 cctattcttt tgatcgatga aaacgaagta actgctggac atgctgccag tgtaggacga
     8221 gttgatccag aagaaatgta ctatctgatg agtagaggat tgcataaaga agaagcagag
     8281 cgcttagtga ttcgaggatt cctcggttct gtcttaacag caatccctgt agaacaagtt
     8341 cggaaagaat tagtcgaagt aatcgaaggg aagttgaatg gatgatcgat gctaaaaagc
     8401 tacgcaatga ttttcctatc ttatttcaag acgtcaatga tgaaccttta gtttatctgg
     8461 ataatgcagc gacgacccaa aagccaaaac aagtactcca ggcattagcg tactattacg
     8521 aacacgataa cgccaatgtt catcgcggtg tccatacgtt agcagaacgc gcgacccatg
     8581 attatgaaca agcaagagaa aaagtacggg cgttcatcca tgcaaaagaa acagcagaag
     8641 tgctgtttac tcgtggtacg acgacaagct tgaattgggt agccagaacc ttcggcgacc
     8701 gctatatcaa agcaggagac gagatagtgc tctcttacat ggaacatcat tctaatgtta
     8761 tcccgtggca gcagttggca aaacgaacag gtgctgtctt gaaatatatc gaactaacag
     8821 aagaaggctt cctggatatg gaggatgcag cgaaaaagat tacggacaaa accaaaatcg
     8881 tctccattgc tcatgtatcc aatgtcttag gtgtcttcaa tcctgttcaa gaattagctg
     8941 aacttgtcca tgaaaacggc ggggtcatcg tagtagatgg tgctcaatct gccccgcaca
     9001 tggccattga tgtccaagca ctagactgtg atttctttgc tttcagcgga cacaaaatgt
     9061 gcggaccaac aggtattggt gttttatatg gtaaacgaaa atggttagaa gaaatggaac
     9121 ctgttgaatt tggtggcgaa atgatcgatt ttgtcgaatt atacgacagt acatggaaag
     9181 aactaccttg gaaattcgaa gcagggacac caaatatcgc cggcgctatc gcattaggcc
     9241 atgcaatcga ttatttgcaa aaactcggta tggacaatat ccatcgatat gaagaagaac
     9301 tagccgctta tgtgttgcca aaattacaag caattgatgg attgactgtt tatggtccac
     9361 aagacccgaa ttttcggaca ggagtcgttg cattcaattt ggatgggtta catccccatg
     9421 atgtcgcgac agctttagac atggaagggg tagccgtcag agcaggtcat cattgtgcgc
     9481 aaccgctatt gaagtacctt gaagtatcag cgactgctcg cgcgagcttt tatttctaca
     9541 acacaacgca agacgccgac cgtttagttg aagcaatcat ggcaacaaag gagtttttcc
     9601 aacatgggac tatctaaatt agacaattta taccgtcaag tgatcttgga tcattcttcc
     9661 catccgcatc atcgtggaag cttgactgac tcgacaagca agatcgaatt gaacaacccc
     9721 acatgtggag atgtgatcga attgcaagtc gatgtaaaag acggaatcat caaaaatatc
     9781 gcctttgacg gcagtggctg ctccatcagt acggcaagtg cctcgatgat gacagacgcc
     9841 gtcatcggaa aatcaataga agaagcagaa catttaactg tagactttct acagcttgtc
     9901 caaggaaaag aagtcaaaga tcccgaccaa ttaggcgatg ctgctatgct tggtggtgta
     9961 gctaaatttc ccgctcggat caaatgtgcg acacttgcct ggaaagcact aggaaaagca
    10021 atggaaaacg atggtaccgc agctttccat cattaccaca aggaagagga gtgagaacat
    10081 gggcgtacca gaattagaag aatataaatt cgggtttcac gatgatgtcg aaccggtttt
    10141 cagtacagga gaaggcttga cagaagaagt gatccgtgag atgtcaagaa tcaaaggcga
    10201 accagaatgg atgcttgaat tccgtctgaa atctctagaa acatttaaca aaatgccaat
    10261 gcaaacatgg ggccccgacc tttcagacat cgatttcgat gccatcaaat attaccaaaa
    10321 accaagtgac aaacccgctc gtgactggga agacgtacca gaaaaaatca aagatacatt
    10381 tgaaaagatt ggtataccag aagctgaacg agcttatcta gccggtgctt ccgcacaata
    10441 tgaatcagaa gtcgtgtacc ataatatgaa agatgaattc gaaaagttag gaatcgtctt
    10501 tactgacact gactctgctt tgaaagaata ccctgaatta ttcaaagagt acttttcaaa
    10561 acttgtaccg ccaaccgaca acaaattagc agcgctgaat tcagccgtat ggtcaggtgg
    10621 gacctttatt tacgtgccaa aaggcgtcaa agtcgatgtt ccattacaaa cgtacttccg
    10681 gatcaatgcg gaaaacacgg ggcaattcga acgtacgctg atcattgttg acgaaggagc
    10741 aagcatccac tatgtcgaag gttgtacggc acctacttat tcaagtaaca gcctgcatgc
    10801 cgccatcgta gaaatcttta caagaaaaga tgcgtattgc cgttacacaa cgatccaaaa
    10861 ctggtcagac aatgtgtaca acttagtaac caaacgagca aaagcatacg aaggcgcaac
    10921 cgttgaatgg atcgacggaa acctaggtgc aaaaacaacg atgaaatacc caagtgttta
    10981 cttagatgga aaaggtgcac gaggcaccat gctctccatc gcctttgcag gagcaaacca
    11041 aatccaagat acgggtgcaa aaatgatcca caatgcacca aatacatcta gctctatcgt
    11101 ctctaaatca atttcaaaag acggcggaga agtaaactat cgaggacaag taacctttgc
    11161 caaaaacagt gcgggttcga tctctcatat cgaatgtgac acgatcatca tggacgaatg
    11221 gtcgaaatcc gatacgatcc cattcaatga gatccacaac agccaagtct ctttagaaca
    11281 cgaagccaaa gtctcaaaaa tctcagaaga acaactctac tatctgatga gccgaggcct
    11341 gaccgaatct gaagcaactg aaatgatcgt catgggcttt gttgaaccat tcaccaaaga
    11401 acttccaatg gaatatgcag tcgaactcaa tcgattgatc agttatgaga tggaagggtc
    11461 tgtaggttaa taactatttc attgctcgaa acgttgatat taaagcgttt cgagcgtttt
    11521 tttatttgta ttttaatggt tggctacgtt tttggctacg tttggaatta ttcgcccata
    11581 aattttgcaa atcggtcacc agttttctta acagattgtg gtgtgacatg agcatagata
    11641 ttcatggtag ttttaatatc tttatgacct aatcgttcct gtacttcttt tatcgacgca
    11701 ccactttcaa acaataagct acagtgggta tgcctaaatc catgtggagt aatttgaggc
    11761 tccatcggtg tctttttgta aatccagtct aaccaatcgt ttactacttg ggggtagtac
    11821 aacttgtttg tgtttgtagt gaaaagaaac tgttttggtg aattcgtgtt atagccatat
    11881 aagatcatta ttttcatttg ttcttgttgc caaatacgca gttgtttaag tgtaaaatca
    11941 tcaagctgaa tcgttcttga tgaattttta gttttaggta cttgtagaac tacttgatta
    12001 aactcgtctt gagcgatagt tttaccaatt gttattgttt tattgaaata gtctatatct
    12061 tcccattgaa gcgccaatgc ttcgctttta cgcattcctg tataagctaa taaacggaaa
    12121 aaagtgtaca gtttaactga attattttct ttagtattct gtaaaaacag ttgtaattga
    12181 tctttcgtgt aaaacttcaa gggttgttct tcttcaattt ttcttggaag caatgtttta
    12241 gacataggat tatccttaca gacttccatt gctactccaa atttaaatat ttgagcagtt
    12301 gtttttctca agaaaccata ttgttttaat gttttgtgcc attcattcac ctgtttttgg
    12361 caatagctaa cacttatttt atccaatcgt aattccccaa aagcaggtaa tatatggttt
    12421 ttgcaaaaac gttgagcaac ggcaacagaa gacggtttta ctcttgttct gtagttatca
    12481 atccataatt catacaactc ttgaaaagtt gtaacattga ttttaaactc ttgtttttct
    12541 ttaaagtcta caagtagtct attaagtgct gtttgcgcct cagcttttga tttgaatctt
    12601 ctacgctttg tacgtatatc ttttccagtc ttgggatctt tccctaaata agtttcaaat
    12661 ccccaatatt tttcgccatt ttgtaattta taactaaaaa atgtt
//
LOCUS       DAYYME010000079        12498 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000079 DAYYME010000000
VERSION     DAYYME010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.080"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..1933
                     /locus_tag="ACHBFW_001851"
     CDS             50..1933
                     /locus_tag="ACHBFW_001851"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM9318155.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2008..2292)
                     /locus_tag="ACHBFW_001852"
     CDS             complement(2008..2292)
                     /locus_tag="ACHBFW_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM9318156.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(2282..3016)
                     /locus_tag="ACHBFW_001853"
     CDS             complement(2282..3016)
                     /locus_tag="ACHBFW_001853"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFM9318157.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            3145..3768
                     /locus_tag="ACHBFW_001854"
     CDS             3145..3768
                     /locus_tag="ACHBFW_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFM9318158.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            3960..5081
                     /locus_tag="ACHBFW_001855"
     CDS             3960..5081
                     /locus_tag="ACHBFW_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM9318159.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            5088..8216
                     /locus_tag="ACHBFW_001856"
     CDS             5088..8216
                     /locus_tag="ACHBFW_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9318160.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            8235..11429
                     /locus_tag="ACHBFW_001857"
     CDS             8235..11429
                     /locus_tag="ACHBFW_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFM9318161.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(11575..12282)
                     /locus_tag="ACHBFW_001858"
     CDS             complement(11575..12282)
                     /locus_tag="ACHBFW_001858"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9318162.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         4258 a         2207 c         2638 g         3395 t
ORIGIN      
        1 tacccgtata ttacgaatga aaaaagttta tgaaaagttg gaatattgaa tggaaaaaca
       61 aaaaaggtta aaaaacagcc gctacatcac tgggtttgat gggctccgtt cattagctgt
      121 ggtgggtgtt atcttctatc accttctgcc aactagtatg aaaggcggct atttgggcgt
      181 tccgattttt ttcgtcgtat ctggttattt gatcacggat ctgctgcggc aggaatggga
      241 acaaaatgga aaaatcaata tttggcaatt ttatgtcaga agaatgaaga gactctatcc
      301 ggcacttgtt tttatgcttg tgacagcttc ggcttatatc actttgtttc agcgagggct
      361 gttaaacaat cttcgaggag ttgtggtcag cagcttactg tatgtcaaca actggtggca
      421 aatcaataac ggtttatctt atttcgatcg atttgctaat gagtcaccgt ttacgcatat
      481 ctggtcttta gcagtagaag gtcaaaacta tttggtttgg ccgatcttgt ttgtattgtt
      541 gatggtattt gttcgtaaaa gaaaatggat cttttatact gtgttaggta catccttcct
      601 ctccgccatc ttgatgatga tcttgtatac accaggaggc gatccaaccc gtgtttatta
      661 cggtaccgac actcgtctgt tttctatatg gttgggaagt gcgcttgcgt ttgtttggcc
      721 aagtacgcgt ttaaagaaaa acatcccgaa acaagcgaaa aatattctga atatcgctgg
      781 aggaatctcg cttttggcac tgattctttg cttcttcttt ttagatgatc attacaattt
      841 catctattac ggaggaatgc tcctagtgag tctgttatgc gtagtgctag ttgcagtgac
      901 agcacacccc ggagcaagtt tgaatcgatg gctgacaaat ccagtattta cttggattgg
      961 caaacgaagc tatggcattt atctgtatca gtttccagtt atgatttttt atgaagcaaa
     1021 ggtgactgat attgccgatc atgttttgtt gcatacttta gtggaaatca cattgattct
     1081 aatcatcagt gaactttctt atcgatatgt ggagcgtccg ctagcacgtt ttgattatgg
     1141 tcgtacattc caagtgatga aaggatggtt cagcaaaccg atcatcagta agagcaaacc
     1201 atgggttgtt ccaggcacat tacttgtagc aattgcactc gttggtttcg ttacggcacc
     1261 gaaaaacgct gtaacagccg atcaaaaaca actgcaggca aaaatcgctg aaagtaaaaa
     1321 attagcagaa gaaaccagaa acaattcaga aaacaccact agttcggttg accaagcgat
     1381 ccttgataag tacgagttgt ccaaggcaca agggaataag gcacaaaaat tagaactaac
     1441 ggcatttggt gattcagtta tgttggatgc tgcaacggat ctacaagaac tttatccaaa
     1501 agtagtggta gatggagatg tcggacgaca attatatgcc agtgtaccgt acattgaaaa
     1561 attaaaggaa caagatttgt tgaaagacac tgttctgatc ggactaggaa cgaatggctc
     1621 attctcggaa gctcagtttg atagtgtgat ggatgcaatc ggtgatcgaa aagtatactg
     1681 ggtcaatgtc cgagtaccga cgaagcgctg gcaaaatgac gtgaatgcga tgcttgaaca
     1741 gatgacgaaa aaatacgaca atatgacgct gattgattgg tacagctaca gtaatgacca
     1801 tagtgactgg ttttatgatg atcaagtcca tccaaatcct gaagggatga cacattacat
     1861 tcacttagtc agcgaagcct tgctaggagg aagttcaagt acgaacagca gtgagacaaa
     1921 cgtgacagaa tgatagctac aaaaaacttc aggcaaaaac atttgttttt gcctgaagtt
     1981 tttttatatt taccgtgttt ttaacattca tatagattca ggaagcaaaa cggttgagtg
     2041 tgtctgtaaa taacgttctt tttgttttcg ggtgagtttt ttgaatgcag cttctgcttt
     2101 tgttgcttcg cttcttgaag aaaatacttc tgcatggatc atcttaacag gtcttctttt
     2161 ttgaagacga gtatatttag caccagtacc attgttatgt tcctctaatc gtctagaaag
     2221 atcggttgta tatccaccat aaaatgtctg atctttacag aataatacgt aaaaataatg
     2281 ttcatttgcc atagagcatc tcacgaatct ctgcagtgta ttctccatcc atattgtaag
     2341 tgatcaatgg tggagcgact ttgaatccgt ctaatttgcc ttctttgatt ccttcaacta
     2401 acaaaatatt tgcctctttt ccaggttttg ggtaaacaaa ttggatcctt ttaggtgcaa
     2461 ttcgatagtc ttccatgacc tgaatgattt ctaagaaccg atcaggacga tggaccatag
     2521 taaagcgtcc atttgttttt agtagcttac ttgaaatatc gatcacttct tctaacgtcg
     2581 tatgaatctc atgacgggcg attgccaggt gtttattagg gttcgtttta tttgttggca
     2641 gccctttgaa ataaggtgga ttacagacga ctaagtcaca agaattatgt cgaacgacag
     2701 ataagctgtc tttcagatcg atggtgtgga cagtcatttg ttcatttaaa tggttaagcg
     2761 cgatactacg tcttgccatg tctgctagac gaggttgaag ttcgatagcg tcgatagccg
     2821 cctgggttcg tttacttaaa aaaagtccaa ctgcgccatt tcctgcacaa aggtcaacaa
     2881 tcttcccttt tttaggtaca cgggaaaagt gagcaagcag cacagcatct aaagaaaaag
     2941 aaaacacttt ggaactttgg ataatcttga tatcttctgc gtataattgg tcgattcgtt
     3001 cgtcttcgtg aagcatggat tccctccaaa atgaaaaaaa tagtcgtctg tattatacta
     3061 tttttattaa aaagaaccaa ggtggcttgt caaacctcta ttttttatgc atactatgac
     3121 cataagaagg gaaaggaaga gactatgttt tatcgattta tgcgcggggt agttcgagta
     3181 ctattagcct tagttaatgg aaatgcccgc tatgagaata aagaagtttt accgcaagat
     3241 gagaattaca tactagttgc tccccatcgt acttggtggg acccattata tctggcggta
     3301 gctgcagcac ccaaggaatt cagctttatg gcaaaagaag aactgttcaa aaatccagtt
     3361 ctgcgattta tattaactcg atccaatgct tttccagtta aaagagaaaa tccaggacca
     3421 agcgtcatta aaacacctgt aaagattttg aaatcgacaa atttgagttt gatcatgttt
     3481 cccagtggga caagacatgc tacagaactt aaaggcggaa tggcattgat ttccagaatg
     3541 tctaaagcga aaatcgtgcc agctgtatat caaggtccgt tgacacttgg ggatctgttc
     3601 aagcgtcaga gagtgacagt gcgtttcggc gaaccaatcg acttatcaga tatcaagaaa
     3661 acggataaag aaggtatggc ggagatcgaa cgacgaacac aagcagcttt tgacaaattg
     3721 gacaaagaag tgaaccctga tttcaaatat gaagtcaaaa aaaaataaaa tgataagttt
     3781 atgcgtgggc ggtcttttga atctactgtc ctgttcagtt cagacttttt caatgaaaac
     3841 gagggagaga cgtcaagtct gccctctttt tgcgttccaa tatgtaaaat agaagacagg
     3901 tgtcatgtaa tggatcaaag tgttataata attagaagga cattttttga ggtgattgaa
     3961 tgcgtttttt acatacagcc gattggcata tcggaaaaaa actacaaggt tatgacttga
     4021 ttgaagacca gcgtcatgta ctatcttcta tactagatat cgcgaaagaa gaaaaagtag
     4081 aggcaatcgt gattgccgga gacttatatg ataggagtgt cccagctgtt gatgccgtag
     4141 aattatacaa tgaattgatg gtcgattgga atttaaagga aaagatcccg atttttgcta
     4201 tatcaggaaa tcacgatagc agtacgagac tttcagcggg tacgccatgg ttcagttatt
     4261 cgaactattt tttgcgaaca cagctagcag aagcatttga accagtcgag tttaaaaacg
     4321 tacaattttt ctttcttcct tactttgaac caattgcagc gagattatat tttgaagatg
     4381 acaatatccg aacgatcaag caagcggtag ccaaggtgat agataaaatg caggagctat
     4441 ttgattcaga aaaaaaacaa gtattgatta gccatttttt tgtaatggga agtgtaaaat
     4501 ctgattcgga aacgaaaatc gaagtaggcg ggttggattc gatccctggt gaactactta
     4561 caccgtttga ttatgtagca ctaggacatt tgcataatca agctgctttg agacaggata
     4621 atgctcgcta tagcggttcc ccattgaaat tctctttatc tgagatcaac caaaaaaaag
     4681 gcgtatggat cgttgacctg acaacaggcc tggagttgaa ttttagggag atcgaacccc
     4741 tgcatgatat tgaagaaata acagcgagtt tcaaagaatt gctggatcct gccttttatc
     4801 agtccgtcaa tcgggagaat tacatctata tctatctcac tgatagaacg atcatcccga
     4861 atatgatgaa ccagcttcgg caaatatatc ctcgtatttt ggggattgaa cgggcaaacg
     4921 gtcgagcaga acaaacaaag cgcaaacgaa aagtactaga aatgaaagat cctcaaaaat
     4981 tagcagaagc attttttagt gaaatgacgg aagagcagct aactgcaaac caagaaaaat
     5041 ggctcggaga aacgttgcaa gaaatcatgg aaatggaatg atcaagaatg aaaccgaaaa
     5101 aattacaact taaaaatttt ggtcctttta tcaatgagac cgtagatttt tcacgtttga
     5161 cagaagcacc gctttttttg atcagcggga agacaggagc tggcaaaacg acgatttttg
     5221 atgggatcac cttcgcactt tttggtgaaa cttccggaag acttcgctct ggaaaagaga
     5281 tgcgttcttt gtttgctaca ccagacgaag aaacaagcgt gactttttct tttgaacatc
     5341 agcagatgag ctatgaaatc gaacgaaaac cagaacaagt tttagcaaaa cgaaaaggaa
     5401 acggaacaaa aaaacaggca gccaaagtaa cgttgaccat ttttgatggg aaaggaaaag
     5461 aaataaaaca aatcacaaaa cgtacagaag cagatcagtt gatcagagaa ctgatgaatc
     5521 tcgatgcgaa acagttttct caaatcgttt tactacccca aggagaattc cgtaattttt
     5581 tggtttcatc aagcagtgat aaagagactg ttttacgtca tttattcggt actcaatttt
     5641 tccaacagtt caatgagcga ttaaaagaaa aagcaaaaca acagcaacaa aaattggatc
     5701 atttagagca agagctgcat cttctgcaaa aaaggtttgt tttagtcaaa gaagaggaac
     5761 cagcagcagg gtttgaagcc gttttagccc aatggacaaa ccatcaggca atactcgaaa
     5821 aacaaatcac ggaagaaaaa gaagctctaa cggaacttca ggaaaaacaa aagcaactag
     5881 aaaattccta ctatcgtttt gaaagcttga aaaaaagtta tgaagaaaaa acgatattaa
     5941 taaacaagca aaaggaatta gctgctcaaa aagacgaaat agaggaaaag aaacgatgga
     6001 ttgggtatta tgaatttacg caacgattag tcgaaccaat ccagcgccta caagaaataa
     6061 aagttgaaaa aatagaactg tcacaaaaaa gtagtgagca aacaaagaaa ctagcgatga
     6121 ctacgcagaa ttatgaagag tggcaaaaaa atgaagcaaa ccgcgtacgc cgacaaacaa
     6181 aaatcaaaga agctgctcaa aaactgcaaa acaaacagat gatggtgcca attgtcgaat
     6241 ccttacgtag taaaaaacaa gacgcagaac aattaacaat ttcattggat aaagaagaat
     6301 tgaatgccga gaagctgtat caaaatctcg ctatctatca ggaaagaact cgaattcttc
     6361 aagaaaattt actggatcag gaaaaactgc aagaagatcg tcttgctttt ggacagttaa
     6421 aatatctaga tgaaaagcag caagaaatag aaaaacaaaa agcaaaagac actgggtcgc
     6481 tcgcacagat aagagaagaa ctcaaaaaag caactgaaga aataacaaaa aatagtcagc
     6541 tattgaaaga acagacagaa caagcagaac agctgaaaag ccaatgggca aagcagcaaa
     6601 ttgctcgtct tcagcttttt ttgcgtccag gggagccatg tcttgtttgt ggatcaacgg
     6661 aacaccccat acaacaatta aaacacgaac agccaaataa ggaagatatc ttaaaagtag
     6721 aacaacaact ggcagaagct gagtctgctg tagaaagaac tcagcaaatc attgccaaaa
     6781 gtgaagcaca tgaaaataat cttcaacaac aattatcaga tttagaacaa aaaatcatgg
     6841 caacgaatat acaattggct gaacaagaag aacacttgca acaggctttg aaaaaggcat
     6901 ttcctgagat aacagaacaa aaaacaaaaa caactcttgt agtaatcgaa caatctttag
     6961 atagacgaaa aagcaagatg gccaatgcaa gaatagagct ggaagaaata agacaacaag
     7021 cagaaaagct cgaacaacaa ttagctgtct acaaacagca gcagacagaa aaaaataatc
     7081 agctccagca actgcaagga gaaatcaaaa gtttagaaca acaattgcaa gatacggata
     7141 gtaactgttt agaagaggag atacgacgac ttgaaaaaca gttagaagaa gactcgaatc
     7201 aactagctgt tgagcagaaa gcaggagtgc aattgttgca agatcttcat acgcttcgga
     7261 cacagcttga tcttcaacat gaacaaatag aaaaaataga tgaaaaaata gaaaaggaac
     7321 aagaaagaat agctaaaaag ttagaaagcc agtcgtattt ttcaacaatt gaagaaatct
     7381 gtgcatttgc acaacagcaa gcacaatata aagaaaacca acgtacagtt acttcttatc
     7441 atgaagagca attagtgaca gctgaccggc tgcgtcaatt agaaaatatc cagctaactg
     7501 agacattccc agatacagaa gagatcagcc aacagttaga agaaataaga cgtatgatcg
     7561 aaataaagca agaaaagata tacggtttac aagaacaaaa aattagtaat caaaaaatct
     7621 acgatgaatg tctttcgatt tatcacacaa gccagacgaa attagatgag cttacgcaat
     7681 tacagcagct ttcgcaaaca ttcaacggag aaaatcctaa gaagactagt ttggaacggt
     7741 atgtgcttca ggtttatctt caagaagtgt tacaagtagc aaatgatcat ctccaacgtt
     7801 taacgaaaaa tcgttatcaa tttgagctag cggataccat tggcagttat cgagggaaaa
     7861 ccggattaga aatcaatatc tatgatgatc aggcaggtat gtctcgaagt gcgcataccc
     7921 tgtctggcgg tgagagtttt attgcagcac tttctttagc attagctctt gccgatgtga
     7981 tccagacaca agctggtggt gtagcaatcg atgcattgtt tattgatgaa ggatttgggt
     8041 cattagatga agaagctttg gaaatggcaa tggaagcttt agaaacgatc gaaagtgaag
     8101 gaagaatgat cggcatcatt agtcatgtaa gagaattgaa agaacgagta ttacaacaaa
     8161 taagaattga tacagaaggt agcggacaaa gcaaaatacg ctatcaactt ggataaaaag
     8221 ggagattggt aactatgaaa tggagtattc cagaaaaagt cattgcacgt ggacgtcaat
     8281 acttgcaaga aggccgagtg ttatcggttg tccctgacca agaagaaaac gtgtggcatg
     8341 cagaagtctt gggcagtgaa ttgtatatgg ttgatttgga tggtacagca aaagagatag
     8401 attactgcca atgtccctat tgggaggaac ataaatattg taagcataca gttgctgtgg
     8461 aactttattt acggtcgaaa aatctatctc gtatcatgaa aaaagaccag ccaatgctag
     8521 aaccggtaaa agcaacaagt gagggagaaa tcattgcaaa aggtttttct cgtctaaaaa
     8581 cacaaaacaa tcctcaagcg gtgcaacctt tgattattga ataccaagta gaaacgattg
     8641 agactaatca gtatcaccca gagctttcta ttttggcact ttacttgcgt atcggttcat
     8701 tggcatcacc gaaaaaaaca tatatcgtaa aaaacatcta tgatttttta caagcttaca
     8761 cagaaaaaga aacttacact gttaataaac agtaccagtt tcgtctagat aaacgggcgt
     8821 ttcaaaaaga agatcaaaaa atactcacgc atcttgcagg aagtgctcag acgcatcaat
     8881 tattaggtac gaacggctta caagtcaaag gaaaactaga taaaagatat ctgcttctcc
     8941 cagtagaaca agcccgcttc ctgttagagc agatgaatca gactacacgt ttgcatctgc
     9001 agataaatga acaaaacgat caaggaatac aattttccga tcaaaacaaa cctttatcat
     9061 tcaaagtgga aaaatttgga gaagattatt tgctgcaagc attgaatgat tttgattttt
     9121 tctttatgca ttatcagtgg ggagttatcc aagggacatt ttattctctg acgaaatatc
     9181 aacaaacggt ttatcaaact tggaaacaat tgattcgccg attggagaaa ccagaagtag
     9241 tgttcaagaa aaaagaatta ccgtctttgt ttaaagagat tatccctctg ctgtcagaga
     9301 tcggcgacgt agctattgcc cctgcagtag aagaggaagt agcagatatc cccgtcgaat
     9361 ttattttctt tttccgaaaa gcaaaaggaa aaatccaagc tcgagtcgat ttcgtctatg
     9421 gcgaagtcat ctattcgact gacccgaagc atgaggtctc gacagaacag gcttttcagg
     9481 tgttacgtga cacagtagaa gagaaacgag tacttgatct attcagaatg tatcgctacc
     9541 agaaaaatga aactggttat gaacgtccat tgccaagcgg ggaagaacta tatgcctttt
     9601 tccgcacaga actatctgtc ttccgtcagt ttggtgaagt cagacttgga agaaaattgc
     9661 gagagttgta tttagatgcg cataaacatc aaccgaaaat cgaagtaacc gagaccggtt
     9721 catggctcga tattcgattc gatgtgacag ggatagagga gcaagaaatc gatcatgtcc
     9781 tgcaaagctt gctgagaaat gatgcttttt atacattaga aaatggtcag gttctttctt
     9841 ttgattcaga agaatttcaa caaacaagcc aagtgttgca acaattacgg gaatcgatac
     9901 gtacagaaga aggaacgatc catgtaccta aaaatcaagg attgatcatt caaaatcaat
     9961 tggaaaaaag caatgctact tttagtgaat cgttccaaac gatggtacaa gatctcattc
    10021 atccagaaag ataccaggca cagcttccca aaggattgaa tgcaacgatg agagattatc
    10081 aaaagcaagg ttttcgatgg ttgaagatgt taggtcatta tcagtttggc ggtattttag
    10141 ccgatgaaat gggacttggg aaaacactac agacaattgc ctttttactt tcggaaaaag
    10201 aagaacggaa aagcttctct gctttgatcg tagcgcctgc gagcttaatt tacaactggc
    10261 aggcagaggt gagaaaattc gccccttctt taagtatcca agtaatcaat gggaataaaa
    10321 aagaacgtga agaattactg gctaaagaca cagatatccg agtgacttct tatgccagct
    10381 taagacagga tctagctaat tatcagtcgc aaaaaattga ttatttgatt cttgatgagg
    10441 cacaaatggt caaaaatagc agtacgaaaa ctgcacaagc attgagagaa ttagctgtac
    10501 ctcagcggtt tgcactgagt gggacaccaa tcgagaataa tttagaagaa ttgtggtcat
    10561 tgtttgcaac gattatgcca ggatttttcc cgactaaaac aaaattcagg gaacttgccg
    10621 cagaagaaat tgcgcaaatg attcgaccgt ttattttacg gcgagataaa caaactgtgt
    10681 tgaaagactt gccggaaaaa acagagatga atctatattc tgcacttaca gaagaacaaa
    10741 aaactgttta tttagcttac ttgcgtcaaa tgcgtgaaga aatcacatca atggattctg
    10801 aagcattcaa gaaaaaccgg atcgggatac ttgctggtct gacaagactg cgacaaattt
    10861 gctgcgatcc tcgtctgttt attgaagatt accaaggcgg ttccggcaag ctcgaacaag
    10921 taaaagatct actggtagct gccaaagaga acaaacgaag agttttgctg ttttctcaat
    10981 ttacagggat gctgactatc ttgcaagaag agttagccga gctagggatc tcgacatttt
    11041 atttaagagg cagtacgaaa ccacaagatc ggttaagtat ggtagacgca tttaatgctg
    11101 gcgaaaaaga tgtttttctg atttccttaa aggctggggg aactggattg aatctcacgg
    11161 gagcagatac ggtcatcttg tatgaccttt ggtggaatcc tgccattgaa gaacaagctg
    11221 ctggacgtgc acatcggatc ggacaaaaaa atgtggtaga agtgtggcgt atgatctctg
    11281 aaggaacgat tgaggaacgc atggattcac tccagcaaga aaaacgcgaa ctgttccaaa
    11341 aagtgatcca aggtaatgaa gaacagttga cgaaactgac tgaagaagac atccgcatga
    11401 ttttaagtgt aggagaaatc gaggagtaag agactcgaaa aagcgttcag taatgggtaa
    11461 gaaagccttt ctaaagaagc gattgtttat ttgatttaaa aaatagcaag gaggctggga
    11521 ttccatttta tcccagcttc cttgctatca tttttttatt tctattttta aaggttatac
    11581 aaagattgtc ttcattgtct ctaacagctg atcaaaagaa gaaatctcga tatcgcaaat
    11641 tggagtctgt tcaattttgc gttctcgatg gttgaaccaa agcgcttgcc agttagcact
    11701 ttttgcacct agaacatcat tgtcgtaatt atcgccaacg tataaggtct cttctggaag
    11761 catatgaaat tctttctcag ctagctgaaa aatctctttt tctggttttt ggaaacctgt
    11821 tgcttgagaa atgagcatat tgcgggaagg aatccaattg ttcaattgaa gttgcttcag
    11881 tttttttgtc tgatggtcag tcggcccatt tgtgataatt cctaatggga cattcttttc
    11941 cttcaagaaa tcgagggttt ttttgacctc ctcatgcaag cagatattat caagttcttc
    12001 ctcgtaaatt ttttgaaata agaggccatc ttcttccgta atatgagggt agtctaaatc
    12061 ttttagtgat tggctgatcc gatgtgctcg catatattct agtgtccaat ctcctgccat
    12121 tacttttggg aagttttcat cactatgatg gcggaaccgg atatatagtg gatgcatatc
    12181 tgcatctgaa acaagcggaa cgactctttt gaccgcgttt cgaaatggct gctgctgatc
    12241 atacatcgta tcatcaacat caaacacaac tgatttaatc aaaattttac acgcctttcg
    12301 gttttcattg attttttcac aaatccttga ctattgtaac agaaaaaatt gtctaaaaaa
    12361 gctgataaat aaaggttttt cttactatat ttctatttcc ttgacagaat aaggttttta
    12421 tatttcagtt tttttcacta aaaatcgtga aacacgtcaa taagtttgat gaacaaatta
    12481 atcattatgg tagaagtc
//
LOCUS       DAYYME010000080        12423 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000080 DAYYME010000000
VERSION     DAYYME010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.081"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..405)
                     /locus_tag="ACHBFW_001859"
     CDS             complement(43..405)
                     /locus_tag="ACHBFW_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318163.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(479..859)
                     /locus_tag="ACHBFW_001860"
     CDS             complement(479..859)
                     /locus_tag="ACHBFW_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFM9318164.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            941..2116
                     /locus_tag="ACHBFW_001861"
     CDS             941..2116
                     /locus_tag="ACHBFW_001861"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFM9318165.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2193..2777)
                     /locus_tag="ACHBFW_001862"
     CDS             complement(2193..2777)
                     /locus_tag="ACHBFW_001862"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9318166.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            2978..3976
                     /locus_tag="ACHBFW_001863"
     CDS             2978..3976
                     /locus_tag="ACHBFW_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM9318167.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4088..4597
                     /locus_tag="ACHBFW_001864"
     CDS             4088..4597
                     /locus_tag="ACHBFW_001864"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFM9318168.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4627..5286)
                     /locus_tag="ACHBFW_001865"
     CDS             complement(4627..5286)
                     /locus_tag="ACHBFW_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFM9318169.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5288..6052)
                     /locus_tag="ACHBFW_001866"
     CDS             complement(5288..6052)
                     /locus_tag="ACHBFW_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFM9318170.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6060..6737)
                     /locus_tag="ACHBFW_001867"
     CDS             complement(6060..6737)
                     /locus_tag="ACHBFW_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFM9318171.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6774..8159)
                     /locus_tag="ACHBFW_001868"
     CDS             complement(6774..8159)
                     /locus_tag="ACHBFW_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFM9318172.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSCLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8369..8453)
                     /gene="ffs"
                     /locus_tag="ACHBFW_001869"
     ncRNA           complement(8369..8453)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHBFW_001869"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8476..8991)
                     /gene="tadA"
                     /locus_tag="ACHBFW_001870"
     CDS             complement(8476..8991)
                     /gene="tadA"
                     /locus_tag="ACHBFW_001870"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFM9318173.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9097..9954
                     /locus_tag="ACHBFW_001871"
     CDS             9097..9954
                     /locus_tag="ACHBFW_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9318174.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10035..10370)
                     /locus_tag="ACHBFW_001872"
     CDS             complement(10035..10370)
                     /locus_tag="ACHBFW_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318175.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10437..11807)
                     /locus_tag="ACHBFW_001873"
     CDS             complement(10437..11807)
                     /locus_tag="ACHBFW_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFM9318176.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(11885..12421)
                     /locus_tag="ACHBFW_001874"
     CDS             complement(11885..12421)
                     /locus_tag="ACHBFW_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9318177.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3677 a         2534 c         2092 g         4120 t
ORIGIN      
        1 tttgaaaaaa attcaaactt ctttgttcgc ttaatctgta ttttagaaga aaggcactcc
       61 ctctacttcg taagttccaa gaacagagcg catttgacct ggttcttccc aaatatatcc
      121 ttcgcctaat tcttctcttt gattcttttg ttcagtttca aagacaaaca acggaacatt
      181 ttcctcttta atatgtgtat ttgtcaattc catcaaacta atcttgctaa catcgatatg
      241 aactatttct tttaagaaat taagtatatt cgctaaacca gtctttcctt cagaaagttc
      301 cgctaacgca aattccaact tgtcatcaat tgaatggacc aaaaattttt tagtaccatc
      361 ctccaaatgc aacataattg taccagcaac tcttttgctt gccaaaattt ccacctcgat
      421 caacttgttc tatggcttac ataaaaattc tagcacattt tcagaaaaaa tacacttttt
      481 atttcttatt ttctttccgt tctagttcag ataatgcttc ttctatccaa ataggtaact
      541 gctccgcaat agtggtaaat ccgatcttac gatttttatt cgtgaaatac ctttttctgc
      601 gatatggaac agctggattt gttttttcta acgttcttaa agataaactg atttttttgg
      661 aatattcatc aatgtcgatg atctgaactg ttactggctg tccgactttt aacaatgaat
      721 ggatattttt cgtataaccg gattgtactt ctgaaacatg aataagtcct tgcgtctcgt
      781 tatcaagaga aacgaatgcg ccataaggct gtatccctgt cacttttcct tcaattattt
      841 gtccaatttt ataagtcatc tacttccctc tcttcgcatt tatcattata ctgtaaatag
      901 ctcaaaaaaa gaagcgacga aaatgactgg aggtcttaag atgatcgaat ttgataaaca
      961 gatttcaaga aaagatacag aaagtgtaaa atgggacgca attgctgaga catatcaaat
     1021 ggatgactta cttcctttgt gggtagcaga tatggacttc ttgtctcccg gtggggtagc
     1081 taaagcgttt agtgaataca tcaaacatgg aatctttggt tatgccactc tgtctgataa
     1141 actatatcag gctatcattc attgggaacg ccaacaccat gctgtggagt tgacgaaaga
     1201 aaatatcgtt tttacaagtg gtgtgttaac cagtttagct gcagcagtac aaacttttac
     1261 aaaaccaggc gattctattt tgattcatga cccagtttat cctccatttt cctctattat
     1321 agaaacaaat caacgtcaca tcgttcgaag ctctttgcta gaaaaaaata atcatttcat
     1381 catggacttg tctgacatgg agaaaaaaat cgaagaaaac aatgtaaaag caatgatttt
     1441 atgcaatcct cacaatcctg gagggcgtgt ttggagcaaa gaagaattga aacagttaag
     1501 cgaattgtgt ttaaaacatc aagtgattct gtttagtgac gagatccatc aagatttaac
     1561 tttattcgat catacattta cttccatgct aacgattgat cctaatttag atgatctact
     1621 agttgctttt acttccgcaa ccaagacttt caatctagct gcaatcaaaa attcaatggt
     1681 atttatcaaa aatccagaga tgaaagctga ttttgaaaaa catttagtga tgaaccaaca
     1741 gcacgaaatc aacacttttg gactgattgg aacccaagcg gcttatgaga cgggtggaga
     1801 atggttaagc caactgattc cttatctaga aaaaaatgtt caaacagtta atcagttttt
     1861 tgaacagtat cttccgaaag tgagaataat gaaccctgaa ggaacttact taatgtggtt
     1921 agattttacc gcctatattt cagacgacca agaattggag aacactcttg ttcggaaagg
     1981 aaaagtcgta ttaaatcctg gcatcacgtt tggtccttcc ggtcatgggc atatgcgctt
     2041 gaatattgct tgtccagaag aaactttact ggaaggactt aatcgaatca agtatgcttt
     2101 agagacaatc gattaaatca tttattcaaa ataaaaggac gagaccttac taggtttcgt
     2161 cctttttcat aattactccg taccatacca aactattctg aaatttcaat tgtttcaatc
     2221 actacatcat gtacaggtcg gtcttgtgga ccacgttgta catttgcgat ttcatctact
     2281 gtgtccatcc cttctagtac atgtccaaag actgtatgtc ggaaatctag ccaaggagtt
     2341 ccgccacctt tatacgcttc aatgatttct gatgggaatc cagcaccttc tagttgtcca
     2401 agcatattag ctggcacatt tgtgttattt acaataaaga attggcttcc gtttgtgttt
     2461 ggacctgaat ttgccataga taaagctccg cgaaggttga acagttcacg actgaattca
     2521 tcttcaaaac tttcgccata aatactttcg ccacccattc ctgttccagt tggatcgccg
     2581 ccttggatca tgaaatcagg aataacgcgg tgaaaaatca cgccatcata ataacctttt
     2641 ttggaaagtt caatgaaatt ttgaaccgtc ttcggtgcta gttcagggaa taattgaaca
     2701 gtaatgtcac cacggtttgt tttgataaca gcttttggtc ctttttcact ttttaaatct
     2761 agttgtggga atgccatatt gtttcctcct aatatttgca tacttcataa taataataac
     2821 ataataccta acctctcgta aaaatactag atttgttttc ttttattttt catcactaga
     2881 tacattctat aacttaccgt ttcgatttcg aattcaaaca tttttgagaa gcattctcaa
     2941 ttaaattctg atatagtgtg aaaagggagg aatttttatg gaaatctatg acattacaat
     3001 catcggtgca ggtcctgtag ggatgtttgc cgctttttac gcaggaatgc gccaagctaa
     3061 aacaaaaatc attgacagtc tacctcaact tggtggtcaa ttagcaactt tgtatcccga
     3121 aaaatatatt tacgatattc ctgggtatcc agcaataaaa gcaagtgaat taatcgatca
     3181 attagaaaaa caattaacta cttttaacca tacattccat ttaaaagaag aagttctctc
     3241 gcttactcgt gaagacgaag ttatcgagat tactacaaat aaaggcatcc attattccaa
     3301 agcagtcatt ttagctttag gaaatggttc attccagcca agaaaattga atttagataa
     3361 tgctgaatca tttgaaaacc acggattgga ttatttcgtg aatgatttga tgaagtatgc
     3421 tggaaaaaaa gtcgctattg ctggaggtgg tgattctgct atcgattgga cgttgatgtt
     3481 agagcctatt gcaagcgaag tttacttgat tcatcgtcgc ccggaatttc gtgcgcacga
     3541 acatagtgta tcacgcttga aatcttcatc tgtcaacttg cttactccct atctcatcga
     3601 tggattatca ggcaataatg gggaactgac agatatccgg ttaaaaaaag tgaaaagtga
     3661 cgagacgatt gatttgatga ttgattctct gatcgtcaat tatggattct cttctaattt
     3721 agaacatctt tcttcttggg gacttgatag taccagaaat gccattactg taaaatcaga
     3781 tatgtctact tctattccag gcgtttatgc agctggtgac atctgtacct atgaaggaaa
     3841 agtgaaactg attgctactg ggctcggtga agcacctact gcagtgaaca acgctttaca
     3901 ctatattaat ccaaaggaac gaactcagcc tggtcacagc acaagcttat acgataaaaa
     3961 tcttcgtcct agttgatttt ttatatctct ttgcatttca agttaaaccc gaaagcagtt
     4021 tcttgcattt ttttcatgtt ccgttaacat gaaatagaga aagaaaaaat aaagggggag
     4081 caataatatg gtcgtcaaaa ctgagacttt agaagaaaag gcaagagaac tattgaagga
     4141 acgtggcgta actgtagaag atatcgcaca acttgtcatg tttttacaaa aagactatat
     4201 ttctgatttg actttggcgg attgtactga aagtgtcaac agcgtgctaa caaagcgtga
     4261 agtacataac gccattatca ctggaatcca attagatata ttagcagaaa agaaaaaatt
     4321 gatgagtccg ttgcaacata ttatcgaaga tgacgaaggc ctttacggta tcgatgagat
     4381 cctagccttg tcaatcgtga atgtttacgg tacgatcggc tttacgaatt atggttatat
     4441 cgacaaggtg aaacctggta ttttaaaaga actaaacagc cacgatggcg ttcatgtcca
     4501 taccttctta gatgatatcg tcggagcgat cgccgctgct gctgccagtc gattagcaca
     4561 caaagaacca gaaaaaagaa gtcgccttac tcaataattc aacaaaaaaa gaaagaaaac
     4621 gtctatttag acgagttctt tcttttttgt ttgtttcaag ctattttttc ctaagaaaag
     4681 aaaaacagca aaaaataatt ctaataatac aaaaaataag ataaagctat gcatgaaaaa
     4741 ttcttctgga agtacattga tgattggatc agtaacggga tcgaataacc agtcatcgtt
     4801 gttgaaaaac gtttcatgaa aaaggataaa aaagcgatca aatcctatca tcatgaaaaa
     4861 accgaaaaca actggcagta acatgccgat ttgaaacgga cgaatcaacc gccaaagccg
     4921 atcattcttt tgcaaatatt tgataaacaa aatactcggg atcaaagtga tgaaaaacac
     4981 agcataatcc actaaaaaca aaattttcac ttcataaaag tgatgggctc cgctagcaga
     5041 aactgggaaa tctggcaagc tgaggatggt tttgaaggga ttattgagat aattcattag
     5101 gtgatcaaaa tttttcagta acgtctcttg atctaaagaa gtataatcca aaatttgcaa
     5161 atgtccgata tcaaatacgt atagtggtcg aaagttgatc gtcacaccaa ctgccagact
     5221 gatcagtgtc agaaataggc tgaacaatcc agcatattcc atccactgcc atctcgaatt
     5281 tttcatatta gaagctccat tcatccagtg attgtttcac ataggtagct gggataggca
     5341 acccaggtac atcttcttct tttgtaaaac ctgataaaac gagcaaagta tctatattat
     5401 tgcgtatccc agcttggata tctgtttcat agttatctcc aaccatgatt acttcttctt
     5461 tttgcagccc taatacttca acggctttat ccataatgat ggcttctggt tttccgatgt
     5521 agatcggcgg cgtttgggtt gctgtctcta caaaagagat gactgaccct gctccaggaa
     5581 gaaggccgcg ttcagtaggt atattcttat ctgggttcgt tccaatgaaa gtagcacctt
     5641 tttgaatagc aagtgttgct ttaactactt tttcataggt cagataatta tctaagccta
     5701 ccactacata gtcaggagcc tcttcttccc atgtaaatcc ggcagctaaa atcaagtcaa
     5761 tcaatcctgc ttctccgata acatagactt ttttcccttt tccatctgct ttcataaaat
     5821 cgattgttgc caaagtagct gtgtagactg tctctggagc tacatgaatg tcaaactcat
     5881 ctgctagacg attagctact gtttctggtg acttagtcgt gttattggta acaaacaaga
     5941 aaggtaattt tcttttttgc aattcttcca caaatcgttt accggccggt atgggttgtg
     6001 tcccacgata aatcgttccg tctaaatcaa ttaaatatcc tttatagtcc attcatccac
     6061 tactcttccc tttttctgat cgtaaatgct gtttttgttt tttctacttg tttaggttgt
     6121 tcttgttttt ttactttttc agaggttttt gctgactctg tttttcgatt tttaataacc
     6181 ggttttttgc gattagatgc gactggtcct tttttctcat cgatatacgc acgattatgg
     6241 ttttttttct tttttcggcg ctgagttgtc ttttcttttt taccgccgat tctctcgatg
     6301 acaaaatacg cacaaccaaa attgcaatat tcgtaaagat agtcttccaa ggaatcgatt
     6361 cgctgatctg gatgcgcccg tcgattatcc gcttggaaaa atccttttag gcgtagttgt
     6421 tcataacccc aatctcctac gatataatca tatcgggcta gtacatcact gtaacgttct
     6481 ccaagctttt ctgcattaaa tccttcacga taatcggaca caagccgata atgtctttca
     6541 cctatcaaaa aattactgtc atctaacagt gtcactagct cgcctttgac tttttctgct
     6601 ttaggctcct caacgatttc ttccaaagca gctgtcagtt cttcggtgac tgttgtttcc
     6661 tgttcagctg atgatttttt cttagcaatt tctgttttat gctcaacgtt ctgctgttca
     6721 ttttgttttg cagacatgcg ttcaccacct atctttttta ttataccttg tttttatttg
     6781 attgggtagt gagctttcaa gtaatctcct accacacttc ggataaattc agggaacaaa
     6841 aattcatttt ttccagcaat ctcaatcgtt ggaaaaaatg gaacaaacat caaatgatct
     6901 acggtagtaa aagtataatt tttattcaga tcgattggct tgttcttcca aaagacttga
     6961 tggttaatag aatcatatgt gattccatta tagaccatct gcccaaaaat cttaccgcga
     7021 aatcccattc ccttcatagg atagttacgt aaaaagttac gatttttttc tatttctaaa
     7081 actaagcgga tcagatcatt tccacccaat gtgacattta acaagtgcat cggatgcggc
     7141 aacgcagtgt gaagctgatc ctgattgacc cgcccttttg gaatttcagt taaaaacaga
     7201 ccactgttta atattgctgc ttccgtcttt cctcgttctt tcaccgcctc aaaggctact
     7261 tggataaacg aatgctcatt gaagatatct agactcagag aaaatggcaa atctgctact
     7321 tctttttctt ttaataattc atgcccacgt tctagacaac tatttatttc ttgttcatct
     7381 tctgtaaaag ctgtcattgt ttcggtaggg atggctttgg ctgaatgctt aaggattttt
     7441 ttctcttcat cgatggttaa atggacttca ccgacatact gcccgtactt tccagcggct
     7501 gctatttgga catcattgat tacgtgacct tctctgaata aatgatgggt gtgcgatcct
     7561 aaaataacat caattgctgg tatttctttt gcaatttgga aatcatcctg aatcccgaga
     7621 tgactcatca aaactaaaac atccacttgt gaacgtaaat cttcgactaa ttctggcaaa
     7681 atatcataag gattccgtat gtcccagcca ttcggattat aagtaagcgg aaatggagca
     7741 gtaaatgcaa ttagtccgat tttcgtctgg tgtttcgttg taatgatttt atatggtttc
     7801 gtccatgccg gtggctggag cgtattttta tcgaataagt tatccaggat gatatcaaac
     7861 tcagcatgat cgtataaatg attcaactct tctttggaat ttcctacgcc ttcattgttt
     7921 cctatcgtcg ctgcatcata accaacttca ttcatcagca agatattttc ctgtccattc
     7981 gttgcttctg acaatggatg ccaacgatcc acaaaatcac ctagatctac tgcaatcgtt
     8041 gtcgtttttt ctttcttttc gttttctcgt ttacgttgat ccaaaaacct tctgatcctt
     8101 ggccaatttt ctaaatgaga atgcagatca ttcgtatgta aaatcactat tttttccatg
     8161 taccctctcg cctctcttta tctttatcat accataggca gctttttctt ccattgaaac
     8221 aagctctatt tcagagtttt tcctgccttg aagcaaattg tataagtaca atattcgata
     8281 aatcagtctg gctaaaaaat caggaacatt catttttaca gtactataaa aataaaaaaa
     8341 acagacccga aagtctgttg tcgtttcatt ataatcagac acatggcgga acctgcttag
     8401 tgctgcttcc ttccagacct gacacgcttc acaagcccac cattgtccta gcctttcggc
     8461 aaagaatagt ataccttatt tttcattcgt tgaccagtct ttttttaatt tcttttttcg
     8521 ctggcggagc tttcggaaaa agtcagtcaa aatcatccca cattcgtctt ctaaaatgcc
     8581 accttctaca tatgcttggt ggttaaaccg ctcgtcctcc aaaagattca tcaaggtacc
     8641 ggctgttccg ccttttggat catacgcgcc gaaataaact tctgctacac gagacaaaat
     8701 catcgcgccg ctgcacatag gacaaggctc taaggtaaca aaaagtgcag catcttccaa
     8761 gcgccagctt cccacttttt cacaggcttc cttgattgca agcatttctg catgtgtagt
     8821 agcatcttgc gtggtttctc taaggttata tcctcttcca atcacttttc catccaatac
     8881 aaccactgct ccaatcggaa cttcatgaag catttctgct ttctttgctt ctgccaatgc
     8941 aaggcgcatc catttttctt tttctactgc atcaagattt gtatttacca attacccttc
     9001 cctgcctttg ttttttcttg ttttacttta gcatatgata aaaaggctgt aactaacttt
     9061 ttttctatgg taaaataaat acaagaggtg aacaagatga aaatggataa attacttgct
     9121 ctttacccac aagcacagaa aaaaaactct ccagcatcag aagcggatat cctttctttt
     9181 gcaattgatg atgcttttat ctggatcaaa caagattcat tatctcaaca agaaaccagc
     9241 cttttgaaag cgttattccc tgtaatcaac gatcaaaaaa aacacccatg gtatcactat
     9301 ctttttaaag atgcttcttg tgttcaagaa aaaagctttc gtttgatcca gcttcacgtc
     9361 gaatcaaaag gagaattctt aaaaaaagaa tggcaagata ctattttgga attatttcgc
     9421 tattctgctg atttcttttt ttatacagac acagatgcgc tcttagtcga acaaataaac
     9481 cgtgattatc ttgatgctac tgaaatcaac ggtatttttc tatctttaga tgctgatttt
     9541 gatacgacaa cgtctgtatt cgttggttct ttccattcac ctggcaccga cctcgtccct
     9601 ttgtttgctg aagaaagaaa aattttttta acagagcaaa acaatcttta tactcacagc
     9661 cgtacttttt ctatgcaaga tgtggcttta cattactata caaaagatac tgtaaaagat
     9721 agtaagttga tcaaaagtta catagaactg atcaaacaaa atgaaatgga agtgatcatt
     9781 caagaattat ggaatgaatt aggcaatgtc agctctgctg caaaaacact gtttcttcat
     9841 cgtaatactc taaaatatcg aatcgaaaaa tttcaagaac agtctggttt caatctaaaa
     9901 aaagccaacg atttactgtt ttgctatctt ttattgcttc atgaacataa ttaaagatag
     9961 attaaagggg ttgtgacaat aattttgtca caacccctta tttcgaatga atagtgttca
    10021 aaagctagtt tctcttacat cattttttca acaaaatgca ttagccggtc ttttacatct
    10081 tcggttgaat ctttgattga atcaccataa acagagattt tcttttttcc tgatttgatt
    10141 tttgctaaca ttcccatcaa agaatccaag tcactatctg aaagaccatc tacgatttct
    10201 agtaattttt catttccatc aaatttgtca tagacgaatt tttttacttt cgtccgagta
    10261 gtcatatgat ggatcttttc cattactttt cccgatacaa cgactgctgt cgctacactg
    10321 gcagcggctg ctacgcttaa tccgattgtt acttttgttg atgttttcat aagtatcagc
    10381 tcctttttat ctatctccat catagcatag acaaaaaaat aaacgaacta atccactcag
    10441 tgaaatctca atcctttagg gaaaaagttc tttactgttc cttttacttg tttcccaaat
    10501 ccgacaggta tcttatctaa tactagcaaa acccatcctt gattcccatc acgttgaaat
    10561 gtttctcctg ctgtgtaaga ttgccattcc ttttgagtga tcggcaagca aggtatattc
    10621 tctatctttt ttgtagcaag cgctaatgca tagctaggtt caaatcgatt ctttttgaaa
    10681 tctcctaaat gcaaacctgt acgtacgact tttaaaccat cgagagaagg ggctaactca
    10741 ggaacttccc aaagatggtc gttgaacact aaaagtcttc cagtagcttc ataatgaaaa
    10801 tcatttgaaa actccgtcca gagtttttcc tgctcttttg tcatttgcac tttacttttt
    10861 tttcgtgtct tcttttcttt atgcatctga ttttgtccat gaaaagtcag ctttgcaaca
    10921 aaatgtcctt caccctgatc tttatgaggc catatacgta ttgttttttc caaaccagct
    10981 acgcttcccc attcagaacg tcctgaacta acggattgag tcaaaggaat ctcctcaatc
    11041 gttactggat aattttctac aagccagcta atgatctctt cattttcttc tggtgcaaac
    11101 gtacaagtcg aataaatcag ttgtccttta ttttttagca tttttattgc tgaagacaaa
    11161 atctcttgct gacgtttttg acagtatagc ggagattctt ctgtccactc ttttattgca
    11221 tttggatctt ttcggaacat tccttctcct gaacaaggag catcaaccac gatccgatca
    11281 aaaaagcccg aaaaatgagg gacaagttct gcgggtgcat gatttgtcac tattgcgttc
    11341 gaaactcccc atcgttctat attttctgat aaaatcttcg cacgtttcgg aaagatctca
    11401 ttcgtcacta ataacccttt ccccttcatt tgagcagcta attgggttga tttaccaccg
    11461 ggagccgcac acaaatccaa aactttttca ccgggttttg ctgcagcagc tgttccaacg
    11521 atcattgcac ttggttcttg gctatattca tacccagctt gatggaggaa agactttcca
    11581 ttgacagtgc caagaaatcc ctcattgctg tagggagcgg gttgtaattc ttcgctatgg
    11641 taagtttgga ccatgtccaa tcctgccggt tttagtggat tccaccgaaa tccttttttc
    11701 acgcttcctt gttcaagcgc agaaaaaaaa tcggatgctt cttcgcctaa aagaagacga
    11761 tattttttga taaattgctg tggtaacgtt gtcgcttcct ccttcattcc aatgtttcgt
    11821 tagacatttt acaatctttt ttacgaaaaa taaaggtggc tgatctactt tttcatgttg
    11881 ctgcctaagg tgactgttcc tttaagatat tttccaagta ttcatccgtc acctcgtttt
    11941 tatcccaaac agcgttcatc atttcaagaa aatcttgaga agaatcatct gagcgcaaac
    12001 ttcccaatgc attaagccat accaattcaa gcgacttttc ttctttgatc ttcgtgtgac
    12061 ctttgacata tgagaaaaat ggccgcttaa atagcgaatc catgtctaaa tcagattttg
    12121 caatgatttc acgaatactt tcatggatag aaaaaaaatc tttcaataag tagacaatgc
    12181 ttaaaacatc atcggaactg cattcagcta agtagttttc aaaataaaca gtgatttctt
    12241 ctaatatctt ctcttttaac ccgctgattg atttgaaatg tgtataaatc gggtatgtag
    12301 aacaaccgca gaactctgct aactctcttg cagtaattga cttcattcca tattcttcca
    12361 cgtataccat acttttgaac agcaaatatt ctttactata atttgttact ctccccatca
    12421 ttc
//
LOCUS       DAYYME010000081        12383 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000081 DAYYME010000000
VERSION     DAYYME010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.082"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(6..812)
                     /locus_tag="ACHBFW_001875"
     CDS             complement(6..812)
                     /locus_tag="ACHBFW_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318178.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            950..2464
                     /locus_tag="ACHBFW_001876"
     CDS             950..2464
                     /locus_tag="ACHBFW_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFM9318179.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(2867..3850)
                     /locus_tag="ACHBFW_001877"
     CDS             complement(2867..3850)
                     /locus_tag="ACHBFW_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFM9318180.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(3855..4502)
                     /locus_tag="ACHBFW_001878"
     CDS             complement(3855..4502)
                     /locus_tag="ACHBFW_001878"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFM9318181.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(4587..5219)
                     /locus_tag="ACHBFW_001879"
     CDS             complement(4587..5219)
                     /locus_tag="ACHBFW_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9318182.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(5235..5786)
                     /gene="coaC"
                     /locus_tag="ACHBFW_001880"
     CDS             complement(5235..5786)
                     /gene="coaC"
                     /locus_tag="ACHBFW_001880"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFM9318183.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(5783..6520)
                     /locus_tag="ACHBFW_001881"
     CDS             complement(5783..6520)
                     /locus_tag="ACHBFW_001881"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFM9318184.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(6517..7506)
                     /locus_tag="ACHBFW_001882"
     CDS             complement(6517..7506)
                     /locus_tag="ACHBFW_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM9318185.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            7764..8306
                     /locus_tag="ACHBFW_001883"
     CDS             7764..8306
                     /locus_tag="ACHBFW_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM9318186.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            8323..9408
                     /locus_tag="ACHBFW_001884"
     CDS             8323..9408
                     /locus_tag="ACHBFW_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9318187.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            9420..10226
                     /locus_tag="ACHBFW_001885"
     CDS             9420..10226
                     /locus_tag="ACHBFW_001885"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9318188.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            10227..11054
                     /locus_tag="ACHBFW_001886"
     CDS             10227..11054
                     /locus_tag="ACHBFW_001886"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9318189.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            11051..12124
                     /locus_tag="ACHBFW_001887"
     CDS             11051..12124
                     /locus_tag="ACHBFW_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9318190.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         3869 a         2237 c         2562 g         3715 t
ORIGIN      
        1 atgttttatc gtttgatttt tttaggaata aaattcttga tgaacgtaaa tttttggtaa
       61 tcgtttcgac tatagaggta accaaataca ctgaaaacaa ctgctccaat aaaattaaca
      121 aataaatcct tcatagtatc catgattccc acgtctaagt aaccattttg gatgattgtt
      181 ttatctgttt taccatctgc attttgactt tcaatgattg tccgagtgat atctggaaca
      241 tgatagacag tgtttccgtc tttacttaac tccacactat tgatttgctg gatgatcgta
      301 tctttttgca tatcgaattg cagtaattta tctgctgaaa attccacaaa ctcccacata
      361 acaccaactg tcatcgaaaa acagaaggaa acaactgcta gtaataaagg agtcaagttc
      421 atggtttttg tatttttatt gagcaaataa acaagggaaa agccgacacc cccagctaaa
      481 aatccgctga atgcatgtag aaaagtatcc cacattgaaa aatagccata aaaatcactt
      541 aactctccta aaactgttga actaaaaata aaagataaca aaatgatttc tagcatattt
      601 ggaatctgta tctttaatat cttttccaga aaataaggga tttcaaataa aattaatgtc
      661 agtaataaaa gcattgtatt tccccagtgt tggttccaga cctgatccac tagcataata
      721 acgacgatga tacgtaataa aatatagatt ccgtttgtga ttctacctgc agtttttgta
      781 ctgttcgtaa aaaaggtttt caaatgattc ataaaataac tccttcatgt atatccttaa
      841 ttatactaag aagcgtgtgt ttaccaaagc tatatgactt aaagaaaaac agagttgata
      901 ttgtgaaaaa aatcacttaa tatagtataa ggagaaaagg gggatacaaa tgaaaaaatt
      961 agttgctggt ttattaacag ccgttgcact tcttgctatg agcgcatgcg gcgcagatca
     1021 gaaaaataca gcgcagacaa gttctagtac gaaggaatct gccgaagtat catcaggcgc
     1081 ttctgaacaa gtatatacag atccaaaaga aatgaaagaa tcttatgatg tcgtgattat
     1141 tggagctggt ggtgcaggga tggcagcagc tatcgaagca aaagatgctg ggttgaatcc
     1201 agtaattttg gaaaagatgc ctgttgccgg aggaaacaca tctaaatcat caagcggtat
     1261 gaatgcttca gaaacaaaat ttcaaaaaga agaaggaatc aaagactcga atgatgcctt
     1321 ctatgaagaa acgctaaaag gtggtggagg aacgaatgat aaagaaatgc ttcgatactt
     1381 tgtcgatcac tctgctgatg cggtcgattg gctagatcaa aacggtatca aactggacaa
     1441 cttaacgatc actggaggga tgagtgaaaa gaggacacat cgtccgtctg atggctcggc
     1501 tattggtgga tacttagtgg aggggttatt gaataacgtc catgagcggg aaatccctat
     1561 ttttgtcaac gcagatgtga cagacattaa gaaaaatggg gaaacggtca atgaagtgac
     1621 tgtacatgtt caaggagaag aaccaaaaga ggtcaccgga aaagcaatcg tagttaccac
     1681 aggcggtttt ggtgccagca aagaatttat tgaaaaatat cgtcccgatt tagctgatta
     1741 tattacgaca aatcaagaag gatcaactgg agatggtatc accatgattg aaaaattagg
     1801 tggacaaacc gttgatatgg ataaaataca aattcatcca actgtgcagc aggatgaggg
     1861 ggtcttgatt ggtgaggttg ttcgcggaga aggagcaatc ttggtcgatc aagaggggaa
     1921 aagatttgtc aatgaaatgg atacaaggga taaagtatct gcagcaatta cagcattgcc
     1981 tgaaaaatca gcttatttga tttttgacca aggcgtgcgt gatcgtgcca cagctattga
     2041 attttatgac caaaaaggat atgtcacaac tggaaagaca atggaagaat tagcagaaaa
     2101 aatcgattta ccaaaagaaa cactagcaaa aacagttgat ggatggaata cggatgttgc
     2161 ggagaaaaaa gatgctcaat tcaaccgtgc cacagccatg gaacatgact tatcgtctcc
     2221 gaattattat gcgatcaaaa tcgcaccagg aatccattac acgatgggcg gtgtgaaaat
     2281 caatacgaac actgaagtat tagataaaga aaaccagcca attagggggt tatatgctgc
     2341 tggtgaagtc acaggtggct tacatggaaa caaccggatc ggtggaaatt cagttgcgga
     2401 gatcattatt tttgggcgac aagcgggcat acaagcagct cgatttgctt cggaaaatga
     2461 atgaaataga acgtaaataa aaagaggttg tgaaatcagc gttttgagct aataccgagg
     2521 gtcagctgat tgaacactgt ttagtgaaga ggttgtgaaa aagctgccct gcatcaagta
     2581 ataagaacag ccaagtaaaa acagcttctt atgtttttaa cttggttgga cttattaccg
     2641 cagatgcaag cttttgaaca ccgtttattc ggaaaaagag cgtgggacaa aagtaaagaa
     2701 cacttttgtc tcacgctcta aacacgtata aacggcggaa gcagaagaaa ttccttcgga
     2761 aataagctga aattcacaaa aatttgaaaa gcaattttcg tgaatttctt cttatttctt
     2821 ggagttaaac gcttctgtcc cagcctcttt tttgatcagc atgattttat tcagttatct
     2881 tcaaaatgat ttttccgatg tttttgtttg cttccatata ttcatgcgct tgtttgactg
     2941 cgctgaattg gaatgtacgg tcaatgattg gccgaatctt gccattttct agaaaaggcc
     3001 cagctttttg cataaaagtt tgtgcgagct ctgctttata ctgatcactt ctaggtgtca
     3061 ataatgtacc aagcagttgg acacgtttcc caatcaatac accaaggtct atctcaggca
     3121 cgatggtccc acctagcata ccaatcaaga cccaacgtcc atcaacggct atagaagcta
     3181 aattcttttc ccagtaagaa gcgccgatga aatccaaaat aacatcgact ccccgacctt
     3241 cagtgatttt tcgtacctct tcttgaaagt cttgtttttt atagttgatc aattcatctg
     3301 caccaagttc tttacaaaag gcgagttttt cttctgatcc tgcagtaacg atgattttcg
     3361 cgtgagaaag ctgtctagct aattggatcg ctgctgttcc cacaccactt gctcctgcat
     3421 ggatcaagac agtttcgcct tcttgtaatt ttccaagcca gtaaagtgtt tgataagcag
     3481 tcaaaaaaac ttctgagatt ccagcagcag aaacatagtc tagcgtatca gggagaagga
     3541 ttgcacgatc ggcaggcatc acagcatatt cggcataacc acctagattg actaatccgt
     3601 atacttttgt cccaggagta aaagtggaga tatcactttt attctcaact acctctccag
     3661 ctacttcgac tcccaaaata gggtagggag gtttcaaaga gactgattct cttttcataa
     3721 tatctgttcg attgacagct gctgtatgga ctttgatcag taattcgcct tgttttggtt
     3781 taggaacagg cacttcaatt tgttctaata catctacaga accaggatgc ttgattgaaa
     3841 tggcacgcat ggatctattc ctcctttgcc cattcttgga atgctgtatt caactctttg
     3901 ctaccttcag aaagtactag agtcgtacga gaaatatccg tttcttccac tagagcggca
     3961 agtacagcag caactgtaga gcgagtgatc gtttttgatg ccttctcatt aggcttagtt
     4021 aattgaatgg aagtaacttc ttcatcgtta gtcaatgtga ctggttgaag aatgacaaaa
     4081 tctaagttag ttcgtttcag ccattcatct gcataatgtt tagtaatata ataatctgtc
     4141 atggactcag gccattttgc tggttcatct gcataaacag cactgaccat cagatagcga
     4201 gagacatgcg cattttcagc tgcgatcact gttttgattg cgccatccaa gtctacttgc
     4261 agaagattct ttccttgaga gccggcagca aaaatcaata catcgatgcc tttgaatgct
     4321 tcggtcattt tttccacgga ccaagttaaa tcaaaagaga catacgaaac attctctttt
     4381 tttactaatg tttgggaagc aacatctctc acccctgcaa aaatttcatg ggaacttgga
     4441 gctaaatctt ttatcaggtg tcggccaatt tgtccgtttg cacctacaac aaaaattttc
     4501 atttggatca tcctttcgac gtcaaatctt tccattcaat agtaacacca aaagagaaaa
     4561 aaatcagaga tcggctacga tgactatcag ttagtaagag aagggtgctt gaagtatctt
     4621 aacaagacat tgcatactgc tgcagtcgca agacctgcga cgattccttc aggtatacca
     4681 cttgtaaaaa taaccgctag aatagctgaa taaacagcat ttaaatttgt tccgatgact
     4741 ttggcataat cttgctggaa taaaaagtag atcatattca tcactaaaat cgtattggtc
     4801 aatgaaccgg aaagtccagc taagaataaa gaaacggaat ctgctttttg tttcattagt
     4861 ttgcgcagac ctttataaac gaaataagga accaccccaa tcaagatgcg aggaataaac
     4921 gcgatcagca aggctttcca gcttccatgt tctgttccga tgactgggat aaacggagaa
     4981 aagacaaaag acaaaggtgt ttggataact gtgctacgga tcatgctgat cagtccaaat
     5041 gtaccgccaa gaaaagcacc tagtcttggt cctaaaacga tcgaagcaat gattacagga
     5101 atatgcatgg ttgttgcatt gattggaccg atagggataa aacctaaagg cgtaacagct
     5161 aataaaatca aaatagctaa aaacatcgca gttaatgtaa agttttttgt atttttcata
     5221 agtactcctt ttttctaagc atttctttca ttaagagtca gcatgacctg ttcgatgatc
     5281 gtctgaatat cagctaaggc accttttcca aaatctccac aagctaaaag agattctctt
     5341 ggttcaattt cctgatagcc gacagatctc aatgtttcca gattacgctg agtgatcggg
     5401 ttttgataca tattcgtgtt catggcaggg gcaatcaatt ttggtgttcc cactggaagc
     5461 gccattgcga ctgttgaaag aagatcgtct gcaatgccgt ttgctagctt gccgataatg
     5521 tttgctgtag caggcgcgat gagaaacaag tcagcttgtt ttgctagttc gatatgattg
     5581 attacctgcg gatttgattc ttgcatgacg tctgtatgga ctgcgcgctt cgacagagat
     5641 tgcaaggtta atggcgtgat aaaatgtgtg ctgcttttag acataatcac atctacctgg
     5701 gcacctaatt tgacgaattg acttgtaaga tcagcgcttt tgtaagcaga aatacttcca
     5761 gtaacgccta gtaagatctt tttcattttg attcctccag tctaattttc tcagtaatca
     5821 atgcagcaat ctcagattta ctttgagctt cttccactgt tccatcttta gataagagat
     5881 agccgtggtg tgtctcgcca tggatttccg tcaaatcatt tgcgaaaata aaatcagttt
     5941 tgttatttat cagactattt tttgcgactg ccagaagaga ttcctttggt acatctacta
     6001 atagtttgaa accaatgatt tttgcttttg gctgccattc acgtagataa gaaatgattt
     6061 ttggtgtttt cttcaaaacg agcacaagat gctcggtgtt agaagagatc tttgaaacag
     6121 tggtatctgt ctgttcattt tccgcaaacc aattgtccag ttcttcttgc gttgaagaag
     6181 tgggtaggtt ttttgccagc tgttcttctg aaaaactaaa agcaggagta aaatcactga
     6241 ctgccatact gtggataatc gcatcgtagt gttctttttt cgttagttct tctaattgta
     6301 aaaataagtc cagtgcagat tcgatcgtat aaatcgttat attggagtgt cttttcggtt
     6361 tcagtgcttg tttcgtagtc acgtaatcga ttgtggtatc cggattggca gctaaataat
     6421 cagctaatgc ttgccctaat cgtcctgtcg aatgatttgt aatagcacgt acttgatcga
     6481 ttttttcagt cgttcctcca gctgtgatta gaatcttcat ttatggtcac ctacatttac
     6541 tagtgttctt ccttggtgtg ttccagcaag tagtttagat gctgtttcag gcaattgatc
     6601 cagagtaatc tcttggacga ccagttcatc tgcaatctgc aaatccgcca tctgttgcca
     6661 taaagaaaga cgttgatcta ttggtacatt gacagaatca attccaatca actggatgtt
     6721 tcgcagaata aaaggcaaga cagtcgcgtt tagtttgatt cccccagcat tgccacataa
     6781 agctactgca ccgttataag agatcaatgg aagaagactg cttagctgct cgccacctac
     6841 tgtgtcgatg acatagtcga tttgctgttt tcctaaagct tttgttgtct ctggcagaaa
     6901 ttcatcaggt cgtacgactt gcaaagcacc tagacttttt agccatgtga cagactcttc
     6961 ttttctggaa aaggcaatga ttgactggaa tcctagttta tgtaacaggg ctatcgctgt
     7021 actgcctaca ccgccagaag caccagtaac gacaactttt ttatcggagg tcataccttg
     7081 acggattaaa gcgttcacac acaacatagc agtaaaccct gctgtaccaa ggatcatcgc
     7141 ttgacgcagc gtcatatttt ttggtagagg aactaaccag tctcctggta ctttttgata
     7201 ttggctataa ccgcctggat gggtgacgcc aagcccaaaa ccagtagcaa gtacaagatc
     7261 ttcttttgaa aaacgtggat tgttactttg gacgacagtt ccggataaat caatccctgg
     7321 agtcatcgga tatgttcgta tcacctgacc agactcgctc attgccaatg catctttata
     7381 attgacatca gaataggcaa tttttaccaa gacttcattt tcagccaact cagtaaatgg
     7441 cagatcactg attgctgtat ggaaaacagg ttcctttatt acctgaaaag atcgaaaagc
     7501 gttcattcgt gagacactcc ttttaaaatt ttcttttatc ataccataaa gcctactctt
     7561 ttattcttgt tcttccgtaa atttagacaa aaattaggtg aaaaataaag aatcaaacaa
     7621 ctagaattta ttccaaaaga taaagaaata ttcagcaaaa aaaattttac aaagggttgc
     7681 ctttttcaat aaatttcgta tactaatacg agtggtagta aacaggaagt ttactattag
     7741 aaaacttcga ttcggaggaa attatggaaa ttggtgagaa gttaagaaat ttgcgcatcc
     7801 aaaagaattt gacacaagaa gaactaggcg aacgcacaga tttgtcaaaa gggtatattt
     7861 cccagttgga acgagattta agttcaccat caatggaaac attcttttct attcttgaag
     7921 tcttaggtgt gactccagaa gaattttttc atcaagaagc agcgaatctg caagtggttt
     7981 attcaaaaga agatcatacg gtttatttcg atgaagaaaa cgggtatgaa ttggaatggc
     8041 tcgtggctga ttcgaatgaa aaagaaatgg agcctgtctt gctgactttt gaaggtgcag
     8101 gagaatataa gacgtttgaa ccttcattat ctgaaacgtt tatttttgta ttagaagggg
     8161 aattagagtt aagcttagga gagacggtct atgtggcaaa agcaggacag tcgatctatt
     8221 atcaagcgac aaaacaacat caattaagga atcatctcaa aaaacgaaca aaagtattga
     8281 ttgttgcaac gcaatcatat ttgtaatata gggggtaaaa acgtggggaa accaatcatt
     8341 tcatttgatc aagtagtgaa acgctatgat gatgaaacag tattaaaaaa agtaagtttt
     8401 gagatcgaac aaggaaaatt ttatacactg cttggtcctt ccggctgtgg taaaacaacg
     8461 atcctacgta ttatagctgg attcaccgaa gctacagaag gggacatcta ttttgaaggg
     8521 aaacggttga atgatttgcc agctaataag cgacaagtaa atacagtctt ccaagattat
     8581 gcactttttc ctcatatgaa tgtttttgat aacgttgctt ttggattgaa gatcaagaaa
     8641 atgtccaaat cagatatcga aaagaaggta aaagatgctt tgcgtatggt ccagcttcct
     8701 ggatacgaaa aacgggagat tagtgaaatg tctggtggtc aaagacagcg tgtagcgatt
     8761 gcacgagcaa ttgtaaatga accgaaagta ttgctattag atgaaccgtt atcggcgctt
     8821 gatttgaaac tacgtacaga tatgcagtat gaactgcggg atctgcaaca acgattagga
     8881 atcacgttta tttttgttac tcatgaccaa gaagaagcgt tggcaatgag tgatgagatt
     8941 tttgtcatga ataaagggaa aatcgtccaa agtggtacac ccgtagatat ttatgatgag
     9001 ccaatcaatc atttcgttgc tgattttgtt ggggagagca atatcgtgga cggtgtaatg
     9061 atcgaagata atctagttga gtttgttggt aagcaatttg aatgtgtcga tggcgggatg
     9121 cgtctaaatg aaccagttga agttgtttta cgcccagagg atttgacgat cacgacacca
     9181 gataaaggaa aactagtcgt aacggtcgat actcagctgt ttcgtggtgt ccattatgaa
     9241 atcatctgtt atgatgaaca gcaaaatgaa tggatggtcc attcaacgaa aaaagctaaa
     9301 gaaggaagca aagtaggatt ggcctttgag ccagaagata tccatgtcat gcgtttcaac
     9361 gagtctgaag aagaattcga tgcacgtctt gacagctacg aagaataggg gggacaagaa
     9421 tgaaaacaat gcgcaggatc tattccgtcc cttatatttt gtggctcggt ttattcgtga
     9481 tcgcgcctgt attaatgatc atttatcaat ctttttttga tatgaatggg caattcacgc
     9541 tgaataatta tctatcttat tttacgtcgg ggacatcgct ttctatgacg atcaattctg
     9601 tatggtatgc atttttgatc acgtttttca cgctgatcat cagttacccg actgcttatt
     9661 ttttaacaaa actgaagcac aagcaattat ggctgatgct gattattttg cctacatggg
     9721 tgaatctgct gcttaaagct tacgcattta ttgggatttt cagtgtgcac ggcagcgtga
     9781 atcatttctt tgattttatc gggatcgggc cgaaacagat attattcaca gacttcagtt
     9841 ttctgttcgt ggcaacgtat atcgagattc catttatgat tatgccaatt ttcaatgcat
     9901 tggaagagat gaatccttct ttgatcagtg ctagtagaga tttaggcgcg aatagctttg
     9961 agaccttccg acgagtgatt tttccgttat cattaaatgg tgtgaaaagt ggtgtccaag
    10021 cagtatttat tccttctctt tctttattca tgctgactcg tctgattggc ggaaatcggg
    10081 tgatcacttt aggaacggct atcgaacagc atttcttggt tacacaaaac tgggggatgg
    10141 gttctacaat cggtgtgatt ttaatcattg ctatgttcat cgtgatgctc ctgacaggcg
    10201 agaagaaaaa aggaggtcgc tcataaatga aaaaatttaa atggtcgtat ctttatttag
    10261 ggatcgtctt tttattccta tatgcaccga tcttctatct gattttttat tccttcaatg
    10321 aaggaggaac gatgaacagc tttacaggat ttacttggga aaattatgaa gctgtttttg
    10381 aagatacacg tttgatcatt atcgtattga atacgttcat gctggctttc ttatctgcat
    10441 tgatcgccac gatcatcggg acatttgggg ctatgggaat ctattacacg aaaaaacgac
    10501 gtgcacggac agcgttgttg agcatgaata acatcttatt agtctctcct gatgttatta
    10561 tcggtgctag tttcttgatt ttctttacac tactaggatt cggattaggt tttcagagtg
    10621 tattgctttc ccatattgct ttcagcattc caatcgtcgt cttgatggtc ttgccaaaat
    10681 tgcaggaaat gaacgattca atggtcgatg cggctagaga ccttggtgct aataatttcc
    10741 aagtagtcaa aaatatcatt ttgccttatc tttctccagg aatcatagct ggatatttta
    10801 tggcatttac ctattctctt gatgattttg cggttacatt tttcgtcaca ggaaatggct
    10861 ttacgacgct ctcagtagag atctattctc gtgcaagaca aggaatcagt ttagaaatca
    10921 atgcgctaag tgcgttagtt ttcttgttct ccatgatatt ggtcattgga tactacttta
    10981 ttagtaaaga aaaccgtcca aaacggctga agaaaaacag aagagtacgc gagggggtgg
    11041 cacggctgcg atgaaaaaat tacaatctct gtttttaggt attctcatca ttattgcggt
    11101 gctgctgctt ggtgttcgtc aacttgaaaa aacaagtggc atgagtggag caaaggtact
    11161 taccatctat aactggggag attatatcga tcctagtttg atcacgaaat ttgaaaaaga
    11221 atatggatac aaagtgaatt atgaaacatt tgattctaat gaagcgatgt tcacaaaaat
    11281 ccagcaaggt gggacaaact acgacatcgc tataccaagt gaatatatga tccaaaaaat
    11341 gatcaaagaa aaactagtac ttccattaga tcattcgaag atcaaaggat taaataatat
    11401 tgatccacga tttttagact tggattttga tccagataat cagtattcga ttccatattt
    11461 ttggggaact ttagggatcg tatataacga taaattcttt gatgcaaatc aaattaagca
    11521 ttgggatgat ttgtggaagc cggaattaaa agacagtttg atgctgattg acggtgcacg
    11581 agaagttatg gggctttctt tgaatagttt aggctattct ttgaatagta agaatatggc
    11641 acagctgaca gaagcagcaa ataaattgaa tcatttaaca ccaaatgtca aagcaatcgt
    11701 ggcagatgag atcaaaatgt atatgatcaa tgaagaagcc tccgtggcag tgacattttc
    11761 tggcgaagct gccgatatga tgtgggaaaa tgaacattta cattatgtga tcccaccaga
    11821 aggatccaat ctttggtttg ataatatcgt tatgccaaaa accgtgaaaa ataaagaagg
    11881 tgcctatgat ttcatcaact ttatgctaga accggaaaat gcggcacaaa atgcagagta
    11941 tattggttat tctacaccaa atgaagaagc aaaagcgtta ttgccaaaag acatttcgtc
    12001 ggacgaacaa ttctatccat ctgatgacac gatctcccat cttgaagtct acaaagatct
    12061 aggatcgaaa tacctaggca tctataatga cctattctta gaattcaaga tgtataggag
    12121 ataatggcaa aactcagtaa gaatggggac ttatcaacgt tctaaagagc taaaaatcgt
    12181 cctttttcta tctgagtttt ccaatcttct accaaaagcg gatgaaacgc cgcttttttt
    12241 gtgttatttt attttttcta ccacgttttt ctaccagaga aaagtggaca aaaccattgt
    12301 aaaatagggc ccgagacggg ctagtaaatc taagattgaa aaagttacag aaggataaag
    12361 taattggaaa ctatgcaaat ata
//
LOCUS       DAYYME010000082        12286 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000082 DAYYME010000000
VERSION     DAYYME010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.083"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..610
                     /locus_tag="ACHBFW_001888"
     CDS             <1..610
                     /locus_tag="ACHBFW_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9318191.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="ACHBFW_001889"
     CDS             723..1001
                     /locus_tag="ACHBFW_001889"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9318192.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="ACHBFW_001890"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="ACHBFW_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFM9318193.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="ACHBFW_001891"
     CDS             1458..2213
                     /locus_tag="ACHBFW_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9318194.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="ACHBFW_001892"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="ACHBFW_001892"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFM9318195.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="ACHBFW_001893"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="ACHBFW_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFM9318196.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="ACHBFW_001894"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="ACHBFW_001894"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFM9318197.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="ACHBFW_001895"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="ACHBFW_001895"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFM9318198.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="ACHBFW_001896"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="ACHBFW_001896"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFM9318199.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="ACHBFW_001897"
     CDS             6950..7492
                     /locus_tag="ACHBFW_001897"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFM9318200.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /gene="atpA"
                     /locus_tag="ACHBFW_001898"
     CDS             7515..9071
                     /gene="atpA"
                     /locus_tag="ACHBFW_001898"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFM9318201.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="ACHBFW_001899"
     CDS             9085..9987
                     /locus_tag="ACHBFW_001899"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFM9318202.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="ACHBFW_001900"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="ACHBFW_001900"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFM9318203.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="ACHBFW_001901"
     CDS             11430..11852
                     /locus_tag="ACHBFW_001901"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFM9318204.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4086 a         2171 c         2708 g         3321 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttatttgggc tctttgtcaa atgatgttgg taaagagaaa tcttagaata
    12181 atttggacag aacagaaaga agaaaacctc tttctgttct gtttatttgg ttaaaaatgt
    12241 tttgccatga cgaatcataa ttcgtagttt gaaattgtaa aagttg
//
LOCUS       DAYYME010000083        12218 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000083 DAYYME010000000
VERSION     DAYYME010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.084"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(258..872)
                     /locus_tag="ACHBFW_001902"
     CDS             complement(258..872)
                     /locus_tag="ACHBFW_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9318205.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1478..1855)
                     /locus_tag="ACHBFW_001903"
     CDS             complement(1478..1855)
                     /locus_tag="ACHBFW_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318206.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2155..3036)
                     /gene="galU"
                     /locus_tag="ACHBFW_001904"
     CDS             complement(2155..3036)
                     /gene="galU"
                     /locus_tag="ACHBFW_001904"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM9318207.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3079..4098)
                     /locus_tag="ACHBFW_001905"
     CDS             complement(3079..4098)
                     /locus_tag="ACHBFW_001905"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM9318208.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4731..5735)
                     /locus_tag="ACHBFW_001906"
     CDS             complement(4731..5735)
                     /locus_tag="ACHBFW_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9318209.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(5748..6797)
                     /locus_tag="ACHBFW_001907"
     CDS             complement(5748..6797)
                     /locus_tag="ACHBFW_001907"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9318210.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(6814..7635)
                     /locus_tag="ACHBFW_001908"
     CDS             complement(6814..7635)
                     /locus_tag="ACHBFW_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9318211.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7622..8401)
                     /locus_tag="ACHBFW_001909"
     CDS             complement(7622..8401)
                     /locus_tag="ACHBFW_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9318212.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8418..8888)
                     /locus_tag="ACHBFW_001910"
     CDS             complement(8418..8888)
                     /locus_tag="ACHBFW_001910"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9318213.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(8900..9319)
                     /locus_tag="ACHBFW_001911"
     CDS             complement(8900..9319)
                     /locus_tag="ACHBFW_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9318214.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9400..12177)
                     /locus_tag="ACHBFW_001912"
     CDS             complement(9400..12177)
                     /locus_tag="ACHBFW_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9318215.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
BASE COUNT         3556 a         2585 c         2006 g         4071 t
ORIGIN      
        1 tctctttttc cactactttt tatttttctg acactacata ttcttataac tcatttctca
       61 taactgtgaa acaacttata aaaataacaa actttaaaca aaacaagctt aaaatcaaag
      121 atgatccatc ttaaaataac taaaacaaaa aaagtagtcg ttgttaatcc tcattaataa
      181 tcgttttctt tttttcaaac gctccatttt tatgtggaac attatttttt taaggaactt
      241 tgtcaacatg tggataacta ttttccactt gtgtcctgga ctagctgttt tgctgtaaaa
      301 tacaaaactt gctgcagcaa aaagatcgtt gcaagagagg ctgttccgca gatcgctgga
      361 tctagatttg gcaaagtcaa gactgcatca tgtaaattgg cgtggttgtc caaaaccaaa
      421 tcagcaaatt caaataatcg caaaccactg ctatgttgcg atttgatact tcgtgacaaa
      481 tcaaatccag taacaatgat taatggatga ccattttctt tgatccactg agctagttcg
      541 atgattgccg ggtttcttcc ttcattcgaa agaagaagaa aaatgtcttc tgatttgaca
      601 gaaagctgat caataattgc ctgtcctgca ccttctagcg tttcatagac acctccggct
      661 ggattttcaa tcaatatact tggtacaaga tccggggttt gatcccaaaa ttcaaatgct
      721 acaccactta gtgtgcggct agcatatatc tgcaatctac caccttgatt aatttgtttc
      781 gcgatccaag tgccagcttg aagcatcagc ggctcttcta tccttaattt ctcttccaat
      841 tggtgataat agtcaaaata acataaactc atttatctct cccgcctagc tatacttgac
      901 atgaatagaa acagctgcct aatctaagga agtacataaa aaaacagtag ttagacagta
      961 acacatgtgg tctttgctgt ttcaaacaca aaaaaggcat tgaacgaata cgcccaatac
     1021 cttgacatag caaataaaac aacgtgcaga ccatggaaaa tatctacata ggggtagaat
     1081 tttcacccta gattaggtac aataaattag gtcgatgcac tgcattgact atggcaaaat
     1141 gatcggtcag ggtgtatgtg tgtgactggt cattccctga ttcttagcgc caactaaggg
     1201 tcagggagcc tttttttatt cagctgtttc tatctgaatt tattgtaagt aatctcttat
     1261 ccaagtgcaa gcaagaaaat acaaaaaaac attggtatga tttatatata tataaataac
     1321 ataaaaaaag gaatggctta tcccgataca acaagcattc ctttaaaaaa gattctttta
     1381 tataagtgaa aaaagctttt ttccaccaat taaataaatc tctcatctca atagtttatc
     1441 cattatttta tctttttttg cgtataccta ctaaatatta ccgtcttctt ctcgttcttc
     1501 tactttgttc gagagaatct gtactagaaa aagtagttgt atgtctacct tttctgccta
     1561 tccgagactt tcttttcata tatgggatat aaatgattag atacagtaca gcaaaaaata
     1621 aagcaaatac gcaagcttcc caactaccta acgccaattc aaccatcaag gtagcaaccc
     1681 caacggatgc tgcataccag ataaaaatca gaaacgtttc tttttttctg accatgcgaa
     1741 acatgataaa tagactaata agcaatagtg gcaacacaaa tatccaattg cttccttcca
     1801 tcagccactt gagtatatca agcagcgact gtgcaacagc gactaatttt cccatagtga
     1861 acacacacct ttttttataa ttaaaaaatt cgccttttta ttctactatg aatcttctgg
     1921 cttggctttc atttctctta tgtaatcatt ttcttttata caacttaaaa aaaaattaaa
     1981 ccattagcat aatattttct gaatctattc acgaaagtct ttttctatct ttcgttttcc
     2041 acgactcaac tgatctagag tcaaaaaact aaaacgacag ttgcgcaaat gacgcacaag
     2101 ctgccgcttt aataacaaat agcctttcat tctcagcagg ccacttattt ccagttaact
     2161 ttctgttctt agctttttac tcaactggat aatatatgca cttaatgaag tcccaatttc
     2221 cgaatgttga agtccatatt caatatttgt cttcatatat cctaacttat cgcctacgtc
     2281 gtatcttttt cctttaaact ctctcgcata gaccgcctct gtctggttaa gagtctgaag
     2341 tgcatccgtt agctgtatct cgtttcctgc tcctggttct tgagtttcta atatctcaaa
     2401 tattttagga gtcagtagat aacgtccgat aatggctaaa ttacttggtg cttttcccat
     2461 ttcaggtttt tctacaaacc cttttactct gcaacgaccc ttttcgctat ctgttttagg
     2521 atcaataatt ccgtatttag atgtttcatt ttctggtaca cgcatgacgg caacagcgga
     2581 agcttgtgtt tcttcataat ccatcatcaa ttgttgagtc aatgggatat catctttcat
     2641 caaatcatcc cccagcatga cgacaaacgg ttcatttcct ataaatgctt ttgcttgaag
     2701 cacggcatcc cccagtcctc ttggatgaga ttgacggatg aagtgaagat tgacttgagt
     2761 cgtttcttca actaaagcga gtaattctgt tttttccttt tcccgcaaat tgttttccaa
     2821 ttcgatattt gcatcaaagt gatcttcaat gggtcttttt tcttttcccg ttacgatcaa
     2881 gatgtcttcg attcccgaat ccaatgcttc ttccacaata aactggatcg ttggtttatc
     2941 cacgattgga agcatttctt ttgccatggc ttttgttgcc ggcaaaaaac gtgttcctag
     3001 cccagcggca ggaatgactg cttttttgac cttcacttgc tgttcctcct aaacgacgat
     3061 taactattga tcatctattt aacgaagaga aaattcattc tccatttttc cgtcacgtaa
     3121 catgatttct tttgctgctt ttttgatatc ttctccatta tacaaaacat tatagattgt
     3181 ttgagtaatt ggcatctcaa cacctagctg ttgagctagc tctactgctg cttttgtcgt
     3241 tgccacaccc tcaacgacca ttcccatgtt ttctagaact tcttccagct tatgtccctg
     3301 tcctaacaag ttaccggcac gccaattgcg agaatgaaca cttgtacaag taacgatcaa
     3361 gtcccccaca ccactcaaac cgataaatgt cagaggattg gcacccatcg cgacaccaag
     3421 acggctgatt tctgccaatc ctcgagtcat gattgctgct tttgcatcat ctccgaatcc
     3481 taatccatgg atagcgccgg caccaattgc aatgatattt ttcagtgctg caccagtttc
     3541 tacgccaatc acatcattat ttgtataaat cctgaaataa tcattcatga acagtttttg
     3601 tacataggct gctgcttttt gatcaatact tgcagctgta attgttgtga tatcatgtac
     3661 tgccacttcc tcagcatggc taggaccaga taatacaaca attgcctgtc ttttttcttc
     3721 tggaatttct tcagccaaaa cttctgagat tcgtttatgc gtatcttgtt ctagtccttt
     3781 acttgcatgt ataatgattg gcttagtatt cattttacca ataagttcct gtgcgactga
     3841 acgaatcgct tttgttggaa caacaaataa aatagcatct gcgttatcca ctgcttctgc
     3901 caattcttta tatccgataa tcgattctgg gatcgtcaaa tcaggtaaaa aatgctgatt
     3961 tgtatgatac ttattgattt catcaatctg ttcaggtaca tttccccaaa tacggacatc
     4021 atgcccgttt tctgccaatg tttgtgctaa agcagtgccc caagatccag gaccaagaac
     4081 tgcgattttt tgtttcatgg atattcctcc aaataaataa ttgataattt ccattatagt
     4141 atacatttct taaagttggg actaatttcc attgttttta agtgtttgtt tacttctttt
     4201 atcgttttta cttgaaataa tcaatgatat gttacaatag tctacatttt atttttacac
     4261 tcgttatgct ttgcttgttc ctaagaggaa gcagttttga caatgttact accagcatta
     4321 ctattaggag gttaggaaac ttctcctagt gagtattgat ttaaaataca taagagacag
     4381 attgaacatt tctaaatctg tcaggtcctg caacggacag attgtctctg ttgacggttg
     4441 acataggaac ttttgtttta gtcaacattc tggatacaaa acgctatgta aactggcgca
     4501 actcgttgta cttggacttt tactacggat ccatgcaaca actaaatagt actattgcat
     4561 aacaaaaaat ggttggcacc cacatgtccc attaaacaga ttataagttg aactgtagat
     4621 aatgcaaaaa atgtgtttta taacgtaatt gatgccaata ttttaaaaaa ataaagggag
     4681 cagttatcgc tgctctcttt attttttata ttctttttat ccagtctatc ctaataatca
     4741 aactgacgat agtagcggcg tatctctaat ggatggcagt tcaactgctc cacatgagca
     4801 tcgatgcgtt gtgtcactac atgcatcaat aagaaagaca aaacacgtcc gcgatatttt
     4861 tcactgatac cttctactgg atagtctttt gtgtctatca atgtataatt gctgcaaatt
     4921 tttggcaaaa gtttagccac acgttcagat aacactctct gttcatcttc gcctaaaaat
     4981 aatgtgactg gggttgtttc ttctacgatc tctaacgtcc cgtgaagaaa ctcggcagca
     5041 tggatagatt tcgatggaag ccagctttgt tcttcccagt aacacatggc atatgaataa
     5101 acagctcccc attgattccc tgctccaata aaataatgca tactatcacg tcgatgtttt
     5161 tctgcaaatg ataagccaaa tgcatctgct tttttctctg cttccactaa ccctgttggt
     5221 aaataacgtt cgagctgttc atagtattct gaataagctt cgaattctcc attcaaatac
     5281 atcaatcgat ctgctgccat aaaaaatttg atttgttctg ttccaggagc tggataagtg
     5341 accacaaaat cgcatagctg agacaactta ctgttcgctt tatcgataaa tccaatcagt
     5401 gtcgctccta ctttcttgat ttgttctact gctttcacta cttcttgagt tgtaccggta
     5461 acagaggaca aaataacgat agaatctttt gttaatcttt tgtttccagt agtgtaatat
     5521 tctgcagcat gctgtacaaa gacaggcaag ttgcttttcc cattcatata agtgacagct
     5581 tgcatcgagg aagcatacgt tccaccgatt cctagataat agacagcgtc aaatttttcc
     5641 gcataaagtt tatcgatgat ttcttctact tgcggacgta aagctagtgc gccttttatt
     5701 gctttgattt gttcttcttc attgaatttc agcatgattt ccacctctta taatttactt
     5761 ttcaagactg tatcaaagtc ttcaaaaatc tttttgttca agtcaatacc tttagctact
     5821 gcggttttat aagcgaatag ttgaaacgga acgacaagcg ctaatacaga cagatattca
     5881 tcacattcta ttgctaatcc aagtgttttc tcgtttcttg ctttttttgt ggtaatggtg
     5941 agagtctctc caacgtaccg actcatatat ctttgcaact tttgcgaacg ttccttattt
     6001 acactttccc cttcaataaa gaatagcaaa tgcgaagcat ctgcctccag gtatgggccg
     6061 tgcatataag cttctaactc aaatccttgt gacggaacgc ggattgtttc agtcaatttc
     6121 gtctctgctt ctttagctgt tccccagttt ggaccatatc caatggtgac gaaacgctgt
     6181 gctaaacgaa ataaactttg atatccatca aaaaattctt ccgttttttg aataatcgct
     6241 ggtaaatggt tgattatttt gcgcaactgt tgcatgtagt cttgttctat ttttttgctg
     6301 attttatttt tacttattcc aattcctatc cctaagagta gtaactgtaa aacagtcgca
     6361 gaatagcctt ttgtaacgaa gcctactgat tctaccccgg cacctaaatc aataatttgt
     6421 gcagcttcta aagcaagtgg actttgcacg tcatttgtga tagcaacaac aggtaaccct
     6481 tgtttttgta tcatttttac tgcttcaaca gtagaagcac ttttcccgct ttgtgacaca
     6541 gcaatcacag tatcgattga aggagaaagt tttccataat tgaaaaaata aaaaggttct
     6601 tgaatatcga cagtaagatc accataagat tccaatgctg gcttcgctgc taaacaagcg
     6661 ttataagatg aacccgttgc taagatcagt aaattcactg tttttttcat atgttctctt
     6721 gtcgaaaaat cattcttttt caagatcgta cttagtactt ccttttcttc atagatataa
     6781 tcttgcatcg taaacatttt ctctgctcct tttttaaaaa atccctatcc acgaaccaaa
     6841 aattccaaag aaagctagtc cgatcaagat cgttaatggt ttcacttctt tcttcaatag
     6901 ataaaacact atgccaaatg ctccaagaca taagatattt ggaacgatat catcaaaaat
     6961 actttgaaca gtcacagcat tttctcctga accgattttt aaaggaatat tgatggtgat
     7021 catcgtggca gtcattgctc caacgaccat cagtccaata atagacgcgc ctaatgtcaa
     7081 actttccatc ataccgtttg cctggatctt tttcaaaaaa cccgttccta agcgatatcc
     7141 ccaacctgtt gctagatagc gaaacagaat gtgcggaata ttgaaaatca aaatgaacaa
     7201 gatagggcct aaaatatttc cttgcatggc taaagaggta cccacacctg tggcaatcaa
     7261 tcgcaaagtt ccccaaaaga aagaatctcc taaccctgca agaggtccca tcaaagatgt
     7321 tttgatacta tcgatcgcag tcacatcaaa ttcaggatca tccttatttt cttcctccat
     7381 tgcagccgtg attcctaaaa tcagtgtaac gatatgtggg gtcgtgttaa aaaactctag
     7441 atgccgtttc aaagctgctg ccatttcttc tttcgactga tataactttt tcaaaatcgg
     7501 aatcattgca taaacataag ccatattcat ctgacgttca taattccaag aaaactccat
     7561 ttgaaaagaa cgccaaaata cgtgatttag ctcttttttc gttattttgc tgtttttgat
     7621 ctcagaattc ttcatcttct tctacctccc ctgcagttgt ttgaagtggt agttgacgga
     7681 cattcataat attgactacc acaactgcta aaatcgcacc aaaaatagct actccggtca
     7741 caggaatctc taagtaagaa gctatagcaa aacctaagaa aaaatatggt gcaacttgtt
     7801 tgttgatcag caaacgagca agcatcgcaa aaccaagagc tggaatcaac cctgtagcta
     7861 ctgacaaccc cttttgaacg aagtcaggaa tcatatttaa tagatttccg atcgtattgc
     7921 ttcccaccat ataagaaacc attaccacaa caccaagcat caaggaaaga ccgataccgg
     7981 caagcaagtg catatgctcg actcctttat aatttccttc ttctgcataa ttgtctgctt
     8041 tatggttcat aattggaatc aaaagcccta agtaaatatt tttcaatatc aatgtcaacg
     8101 tagcaatcgg caaacctagt aaaagcgcgg tttctgtacc ggctccagct gtaatagcaa
     8161 aagccgtccc taaaatccca ccggtcacaa catcaggtgg aatcgaagca cctactgaga
     8221 acgatccgat aaatgctaac tctaaagttg cgcccataat gatcccagtt tttacatcac
     8281 ctaacacgat tcctgtaaac agccccgtca caatcggacg agaaagcaac gatgtcccta
     8341 gtgcatattc tgattgtgcg acaaatgcaa caattcctaa taagactgct tgtacaatca
     8401 ttgaggttct tcctttctta caaaacttct cttacatcca cttttttatc tgctggtact
     8461 tgacggattt ctacttctat ttgtttgtca attaatcgtt tcaagcgttc ctcctcttct
     8521 ggtaaaatat ttaccgcttt tccgatgctg cgagtaccag gttttgcctt gatccctcct
     8581 aagttgatgg ttttgatttc tggatacgct tctgccagct tatatgcatc ctcgatcgat
     8641 tccactacga tgaataactt atatttatct gtcaccccgc ttttcaacgc ggcaatcgag
     8701 tcatcaattg atttgatcac tagtttgact ccttgtggct tggctaattt gatcgttgtc
     8761 tttcgtatct cattcgtagg tacatcatca ttagcaatca aaatacagtc tgcgcctatt
     8821 gtttgtgtcc atgaaaaagc tacttgtccg tgtaataacc gatggtcgac tcttgtcaat
     8881 acaatcattt ttcttcctcc taaaattcgt cttcttctat tttctgattg agccgctcgt
     8941 tacaaaactg gatcattttt tttgaatcag ttagcacctg ctggatcaaa acagttgtct
     9001 ctacttcagc gtccaaccga gatactagct caatcaatag tggcaaattc aatccggcaa
     9061 cgagatagac atgatcccga ttgatatatt gtaagaattc gttgttgaca cttccgccaa
     9121 aaagatccgt cagaacaatg atttctttat ctttgtacct cataaaaagc tgccttactg
     9181 tttccgctaa atcaaaattc tcatcagtat agcaatcgaa tacagtaata gaatgatttt
     9241 tcccatagat cagctctaat gaatctaata tccctgaagc aaattttcca tgggaagcta
     9301 agataatttc tctatccact ttttcatctc ctcttttcac ttacacatac taaaaaagca
     9361 agttgcatgc caaaattagt ggcaacaact tgcttatgtt caaaattctt cttctatcga
     9421 gagaaactct gtatcttcac tcaaaatatc atagatatag gcaatctcag aattggggat
     9481 catgacacta taatgctctt caatgacact aaacgcagat ttaatagcag ataacccttc
     9541 tttttgacac tgcttccatt tctctaatcc ttgataggtt tcgattggca tttgtcggat
     9601 caatcgttca atgagacagc tcacatggac atacaacgct aattttttgg gattgctgat
     9661 gcttcgattc aactgcacct ctaattctct cataaaatga tcgatttctc ccatcacctt
     9721 ctctgtatct agaatagtca cggaatccag tactttttcc aatgaaaaat tacgaatgat
     9781 gcgctgattg aatatttcct tctctgaagc attcatagta gtgtccaacc attcagctag
     9841 tacagacatc ttttcccccg agatgaggtc ttctaatgaa agatagtcga tattggtgat
     9901 atcaggatct gtggtgccaa tcattcctac gacttcgtat atggaaaata atgggtcact
     9961 gtttttctta tcttttaatt gctgatattc atatgggatg attttcaact gacaagccgt
    10021 aggtaagctt ttttctagca gttcacttaa gtggaccgcc gtaccgattc cagtgaagca
    10081 agtagtaatg agtgctttgg gtttgttttc ctctggatag agcagtcgcc attctggctg
    10141 gatctgtcga acagtctctt ctgctatctc ttctaatgat acattttttt gcaggcattc
    10201 ccctactgtg attgctaaag gtgtcgtcac attattcaaa atcaaaagcg gtacggataa
    10261 ctgtttttta aaaaactgat ggatttcttt cagagatccc atgtctacta atatgatcaa
    10321 tccatttgaa acatcgctgt tttcgcaata atcaaggatt tctgaagcaa tctgctgtgg
    10381 tgtgacatct agtggcatat caaatgattc aaatagatcc tcttgtaata aacgatttac
    10441 tacatttgcc atactgctag cagttgcata accgtgagca gcaatcatcg cttttggcag
    10501 tcgtttttct tttgaccatt tcgctttttt tagatagatc gtcagtaaaa tccgatccat
    10561 acttgtgtct tcaatatcta atttgatctt acttaattcc agaattcgtt ggacatagtg
    10621 ataactagat ggataagact gagaaacttg ttgttctaat tctttgatta gttggataat
    10681 atcatgttct tccggtaacc atttgatagt gcttcgctga tagagatagt aactcaaggc
    10741 atagacactg tttccatcga atttgatttg ataggcagtt tccatttgtc gaaaggtctc
    10801 tcggatagat tgtgtccaat aaaccagcag ctcgtgtttt tcttgtctac tggtctcaaa
    10861 cagcaaaaaa tcaaataatt gaatcacttc ttgctttaat tttccttcgc aagaatatag
    10921 cagacagtta tttttcttga aatcaattag gatttgctca aaacttttaa cgattcgctg
    10981 ctgttcgggc tgttgcttct ccattaattg ctctaaggtc tggtcttgcg taatatagat
    11041 tccttgctgt gctgtatttt tagtaatagg ctgcgcaagt agttcttttt gcaaatgatg
    11101 gatcgtgaca tggatttcag gctgttcacg ctgttcagca tatgcttttg ctgctgaaac
    11161 tttgacgata tttttcagct ctcctatgtt tccggataat ttctgacttg ttaaaagtga
    11221 caaagctggt ccactgactg acaaagggct ccccattttt ctttgttctt cgagaaaaaa
    11281 agagtaaatc agttctagcc gttcattacg actacgctga ttaagtgcag gtagttcgat
    11341 tcggataggt attcttcgaa tgaaagttgt taagaaatga ctagtaatct cttcagtcgt
    11401 agcaaaaaag agacgtgttt tgatccggcg tgacaaatta ggttctccca tacgataaat
    11461 cacaccttga tcaagatagg tgaacagttt ttcctgacct tctggtgtta agcgatgtac
    11521 ctcgtctaaa aacaagatcc caccatctgc tgcttcaaaa gccccttttt tattttcttc
    11581 tgcaccagtg tacgctccct taacatgtcc aaacaaattg ctagtcaaaa gttcaggatt
    11641 atctgcatat tgtgcacaat taaaagtgat gaatggtgca gaatcttcta gcagatcatg
    11701 aagcagacaa tactgataga ccaaattaac taaataactt ttacctgtgc cactttcgcc
    11761 tgtaatcaaa agtggtaatc cgccatctgg atagtaaaga gctgttttga tttgttcgat
    11821 actttctttc aaactggcgt cgtatccgat cataaaagag aataagtctt ttggttgcgc
    11881 gaaaaaaggc tgttcctctt tgagatcatc gatagaaaaa taaaaattcg tgcgtaactt
    11941 aaaaaattgt ttttcaaaag cttctttgtg gaaataaatc actggtcgag aattgatttt
    12001 gactagtagt tgctcatcat ttaactggtt caaataatga ctgactgtgt ttctttttac
    12061 cccaaaatga tcagctaacc tttgtgctgt aaagacctcg ttcatatgtt ctaaatcgaa
    12121 aaaggctgtc tggttctcta agtaacataa aatttccttc ttcaacaact tattcacccc
    12181 tcctccatca tagaaaacgg attcattttg tcaataaa
//
LOCUS       DAYYME010000084        12171 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000084 DAYYME010000000
VERSION     DAYYME010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.085"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..297)
                     /locus_tag="ACHBFW_001913"
     CDS             complement(133..297)
                     /locus_tag="ACHBFW_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318216.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            592..1920
                     /locus_tag="ACHBFW_001914"
     CDS             592..1920
                     /locus_tag="ACHBFW_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFM9318217.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2122..2955
                     /locus_tag="ACHBFW_001915"
     CDS             2122..2955
                     /locus_tag="ACHBFW_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFM9318218.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2971..3708
                     /locus_tag="ACHBFW_001916"
     CDS             2971..3708
                     /locus_tag="ACHBFW_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFM9318219.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3863..4831)
                     /locus_tag="ACHBFW_001917"
     CDS             complement(3863..4831)
                     /locus_tag="ACHBFW_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFM9318220.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5063..5896
                     /locus_tag="ACHBFW_001918"
     CDS             5063..5896
                     /locus_tag="ACHBFW_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFM9318221.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5930..6769
                     /locus_tag="ACHBFW_001919"
     CDS             5930..6769
                     /locus_tag="ACHBFW_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9318222.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6795..7355
                     /locus_tag="ACHBFW_001920"
     CDS             6795..7355
                     /locus_tag="ACHBFW_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9318223.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7554..9275
                     /locus_tag="ACHBFW_001921"
     CDS             7554..9275
                     /locus_tag="ACHBFW_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFM9318224.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEISYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9440..10582
                     /locus_tag="ACHBFW_001922"
     CDS             9440..10582
                     /locus_tag="ACHBFW_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM9318225.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10598..11809
                     /gene="thiI"
                     /locus_tag="ACHBFW_001923"
     CDS             10598..11809
                     /gene="thiI"
                     /locus_tag="ACHBFW_001923"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFM9318226.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4148 a         2041 c         2527 g         3455 t
ORIGIN      
        1 aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat
       61 gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt
      121 ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc
      181 tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt
      241 attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt
      301 attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat
      361 cttattgtat gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc
      421 atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga
      481 agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa
      541 attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct
      601 attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag
      661 gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca
      721 catgtggaga atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa
      781 atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg
      841 aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa
      901 tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat
      961 agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg
     1021 atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg
     1081 aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca
     1141 gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa
     1201 gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa
     1261 gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca
     1321 ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc
     1381 aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat
     1441 gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta
     1501 gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct
     1561 tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat
     1621 acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat
     1681 gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa
     1741 aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg
     1801 gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat
     1861 gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag
     1921 acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta agccagctct
     1981 ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat tctaaataaa
     2041 ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag tagaataaaa
     2101 ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct gaaattaaat
     2161 ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat aaaaaccgaa
     2221 catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt tgaagcagaa
     2281 gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta tatgctaggt
     2341 gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa aggggatcaa
     2401 cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca atttggtttt
     2461 cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga agattggtgg
     2521 acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga aacgaatcga
     2581 tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt gaaatcggtt
     2641 gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg taatgtgttc
     2701 tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg gaatcgggaa
     2761 caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt agatgcttat
     2821 caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta ccagttatat
     2881 tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt agatcaatta
     2941 ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa aaagcatgaa
     3001 gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat gttaggagta
     3061 gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga attcgtcaat
     3121 agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg tctccctaga
     3181 gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt tttctgttat
     3241 cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga atctgttttc
     3301 gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat cattgctcct
     3361 tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa gattgattcg
     3421 ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca gcatgttaat
     3481 aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt gactacgcat
     3541 gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa tgagatcgaa
     3601 agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga aatccgaatg
     3661 caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa aagaggttgt
     3721 gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt tctcaaattt
     3781 ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc tctttttaag
     3841 tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct tcacccgaca
     3901 aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta atactgttac
     3961 agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca taaacaggct
     4021 ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg acaagaagct
     4081 tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct tcgattacca
     4141 aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc ccagcatatg
     4201 ctttaacgat cgggatagtc ccatctacag cggaaatgtc cagtcgcttt gtttctaaaa
     4261 gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc aatgtaggtg
     4321 ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca gaatggatct
     4381 catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt gcgacacgaa
     4441 tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa cgcattgccc
     4501 cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa gcagtttctt
     4561 ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca taagcggaca
     4621 agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag ggaagattga
     4681 agatatcttc aactaataat tctacatgct tggggattgt gatttctgca tctaataatg
     4741 ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg gcgatcgttc
     4801 cgccggtgtg tagtacaagt atttttttca ttttacagac ttccttacga ataattttgc
     4861 atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt gaagaataac
     4921 gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga ctcaaccttg
     4981 acctcctaat acaatggagt atctggttga gtttctttta caagaaagca gccaaggaaa
     5041 tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta aagaacatga
     5101 caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc tacccttagt
     5161 ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga attggataag
     5221 tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca gcggtcaagg
     5281 agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt tagatgttat
     5341 gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc cctcatcatt
     5401 caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt ttgagggtta
     5461 tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc ttgaacgtac
     5521 aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc cacagttgct
     5581 gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc tttttcgatt
     5641 ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta aaaaaagaac
     5701 gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca aagtagttga
     5761 ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg atgcggctga
     5821 aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca atccaaccca
     5881 aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta tgatcaaagc
     5941 tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt tggaatcaac
     6001 aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag caacggggcg
     6061 cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt atgtcgtgta
     6121 taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt ttgaacaaaa
     6181 agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg tttttggtgc
     6241 gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt ttatcaagcg
     6301 actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa tcctccaggt
     6361 ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa aagaaccaat
     6421 ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac atcgctttcc
     6481 cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa aaggtggttc
     6541 aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg aagaaattat
     6601 ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa ttggagtggc
     6661 catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc aatcaaatga
     6721 agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt caatgaaaga
     6781 aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca ttccacaaaa
     6841 cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa gctcatacac
     6901 gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca cctaataaag
     6961 aacaactgca tgaagctgtt atgctgctgc acgaagcatt agataaagca gaagaaaaaa
     7021 tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat gcactgacag
     7081 atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct gatcgaatat
     7141 tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa cctcattatg
     7201 accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat gctccagaac
     7261 ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag aagaaatccg
     7321 ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat ttttgttatc
     7381 tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg aaaatagtat
     7441 gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat ttatgccaag
     7501 cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca taaatgggaa
     7561 ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc gtctttttgg
     7621 ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa aaacgcaagg
     7681 aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa atgcatctgg
     7741 ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac atatctactt
     7801 cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat gaaagttttc
     7861 ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac caaatggaga
     7921 aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa gaacgcaact
     7981 cactagctgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa gtcagagaag
     8041 acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa gtgatcgaaa
     8101 aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa gcccgcgagg
     8161 ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa aaaattccac
     8221 cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc agtgatactt
     8281 acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct gaagatatcg
     8341 caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca gaagttgaaa
     8401 cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat gatatcttag
     8461 aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt gcagagtata
     8521 tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac acatctcaaa
     8581 gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc gaagtggaag
     8641 aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa atttcttatt
     8701 cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc gaaaagcaac
     8761 aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa gcgcaagaaa
     8821 agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa aaacaacgtt
     8881 tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac cgtattgaag
     8941 aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat cgtttagttg
     9001 ctctatgtga agaagatcta gaattattag ataagaagac acatgattta gtagatgcag
     9061 cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca cacgaagaag
     9121 tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac tatcaagaag
     9181 cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc aaacgcatcg
     9241 aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt aaaccagcaa
     9301 gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa attgaaacaa
     9361 gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag aaaagaattt
     9421 caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc caatctttcc
     9481 gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc catcaagctt
     9541 gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc agatcgctga
     9601 tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag aaggagataa
     9661 ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata tgatcatttc
     9721 aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat taggaatcga
     9781 agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat tgaaagaatt
     9841 aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag taggatcgat
     9901 tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc atttccatgt
     9961 agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag aaagagttga
    10021 ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt tcatttattg
    10081 gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa gaaatcagcg
    10141 aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac gtttatcatt
    10201 agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc taatcaatgc
    10261 gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg caccgcatat
    10321 tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct tagaagaaaa
    10381 acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg caagcagtac
    10441 attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa tcagcttaga
    10501 tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc agctttataa
    10561 gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg aaattatggt
    10621 ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca tgcaactagc
    10681 acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg ctgatcggga
    10741 ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa aactagcaaa
    10801 aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg tcgaagtttt
    10861 gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa catttaaaat
    10921 tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga atcaaacatt
    10981 aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc aaccagatat
    11041 tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa cgatcaaggg
    11101 agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt ctggggggat
    11161 tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg aagctgttca
    11221 ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag atctaacagc
    11281 caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta ccgagattca
    11341 agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc gtcgaatgat
    11401 gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt tgaatggtga
    11461 atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca atgaagtaac
    11521 gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca ttgaagtagc
    11581 agaacagatc gatacattta atctttctat ccagcctttt gaagattgct gtacgatttt
    11641 tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat atgaagagcg
    11701 tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg aagagataca
    11761 agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag aggttatgaa
    11821 aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc atgtatttta
    11881 ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa gaagttatgg
    11941 aaaagccggt tcttttttac taactgaaaa ataaaatttt atttagagat caagctacag
    12001 ttgataaatg atataaaaaa gagaatttcc catgaaatag cgaggttgtg acagaagtgg
    12061 gcagcttcaa gaaataagaa ggaatttacg aaaattgctt ttcaaatttt tgtgaaattt
    12121 cggcttattt ttcgaagaag ttacttctgg aacaccgtgt atcagaggtt g
//
LOCUS       DAYYME010000085        12081 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000085 DAYYME010000000
VERSION     DAYYME010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.086"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..528)
                     /gene="frr"
                     /locus_tag="ACHBFW_001924"
     CDS             complement(<1..528)
                     /gene="frr"
                     /locus_tag="ACHBFW_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFM9318227.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAA"
     gene            complement(531..1253)
                     /gene="pyrH"
                     /locus_tag="ACHBFW_001925"
     CDS             complement(531..1253)
                     /gene="pyrH"
                     /locus_tag="ACHBFW_001925"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFM9318228.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1389..2270)
                     /gene="tsf"
                     /locus_tag="ACHBFW_001926"
     CDS             complement(1389..2270)
                     /gene="tsf"
                     /locus_tag="ACHBFW_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFM9318229.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIEYDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2369..3151)
                     /gene="rpsB"
                     /locus_tag="ACHBFW_001927"
     CDS             complement(2369..3151)
                     /gene="rpsB"
                     /locus_tag="ACHBFW_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFM9318230.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3510..3989
                     /locus_tag="ACHBFW_001928"
     CDS             3510..3989
                     /locus_tag="ACHBFW_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM9318231.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4206..5297
                     /gene="phnW"
                     /locus_tag="ACHBFW_001929"
                     /pseudo
     CDS             4206..5297
                     /gene="phnW"
                     /locus_tag="ACHBFW_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            5290..6075
                     /gene="phnX"
                     /locus_tag="ACHBFW_001930"
     CDS             5290..6075
                     /gene="phnX"
                     /locus_tag="ACHBFW_001930"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFM9318232.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6421..8112)
                     /gene="argS"
                     /locus_tag="ACHBFW_001931"
     CDS             complement(6421..8112)
                     /gene="argS"
                     /locus_tag="ACHBFW_001931"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFM9318233.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8534..9481)
                     /gene="arcC"
                     /locus_tag="ACHBFW_001932"
     CDS             complement(8534..9481)
                     /gene="arcC"
                     /locus_tag="ACHBFW_001932"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFM9318234.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9596..10615)
                     /gene="argF"
                     /locus_tag="ACHBFW_001933"
     CDS             complement(9596..10615)
                     /gene="argF"
                     /locus_tag="ACHBFW_001933"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFM9318235.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10706..11935)
                     /gene="arcA"
                     /locus_tag="ACHBFW_001934"
     CDS             complement(10706..11935)
                     /gene="arcA"
                     /locus_tag="ACHBFW_001934"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFM9318236.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3307 a         2519 c         2179 g         4076 t
ORIGIN      
        1 agcagcgatc ttatctactt ctttaatgct gttatctgtc aatgtttgta cgtctttttc
       61 taatccgcgc aaatcatctt cggtaatatc accatttttt tgttgttttt tatattcatc
      121 gatcgcatca cgacggatgt tacgaatcgc gatttttgca ttttctgctt cttttttcac
      181 atctttagct aattcttttc tgcgttcttc tgtcagttgc gggataacta agcgaatgac
      241 atttccatca ttggttggac tgattccgat atcacttgcc ataatagctt tttcaacatc
      301 ttgaagaatg ctcttgtcaa aaggtgtgat cattaaaaca cgtgcttctg gaatttggat
      361 tgatgccatt tggttcaatg gtgttggcgc accgtagtag ttcacagtaa tacggtccaa
      421 aaggctagcg ttcgcacggc ctgcgcggat ttgcccaagt tcacgttgaa gattttgtgc
      481 tgcttttttc attttttctt ttgcttctgt cataattgca tctgccattt ttatttcccc
      541 cttacagttg ttccgatatt ttcgcctaag attgcacggc gaatatttcc gtgttcattt
      601 aaattgaaga caagcagtgg aatatcatta tccatgctta gtgaacttgc agttgaatcc
      661 atgacttgta atcctttggc aataacttcc agatgagtca gttcttcaaa ttttactgca
      721 tttgcatcta ctttcggatc agcagaataa acaccgtcta cattgttttt agccatcagg
      781 ataacatctg cgccgatttc tgccgctcgt aatgctgctg ttgtgtctgt cgagaagtac
      841 gggttgccgg taccgcctgc aaagataacg atccgtcctt tttctaaatg acgttctgct
      901 ttacggcgaa tatatggctc tgcgatttga cgcatttcaa tcgaagtttg cacgcgtgtc
      961 ggtacaccca cgttttccaa cgtatcctgt aaagctaagg cattcataac tgtagctaac
     1021 atacccatgt aatcagcttg agcacgttcc atgcccattt gtgctccgat ttgtccgcgc
     1081 cagatatttc cgccacccac aacgattgcc atttcaacgc ccagctcgtg aacttctttg
     1141 atttcttgga tgatttcttt gattactggt ggtttgattc caaatcctgc atccccagct
     1201 aaagcttctc cactcaattt taagactaca cgctgatatt tcggtgtgac catgttcatt
     1261 cctccaatat tttctattgc cattttacca tacttagatg atttcgacac tctaagactg
     1321 gtttttcttg ttttgcttaa aaaaagggag cgcacaaacc gtgcgttccc gtctgttatt
     1381 caacagaatt attttttcac ttggctcata acttcttcta caaagttatc ttcgcgtttt
     1441 tcgatacctt cgccaacttc gaaacgtacg aatgttttaa cagttgcgcc ttttgatgca
     1501 acgtattttt caacagtcat atcaggatct ttaacgaatg gttggtccac caaagcgatt
     1561 tctgctttga atttcttcaa gcgtccttct accatttttt caacgatatt cgctggtttg
     1621 ccttcgttca atgcttgttc tgtaagaaca gttttttcat gttctaattc agcttctgga
     1681 atttgtgatt cgtttacata acgaggattg atagctgcta cgtgcatagc tacgtctctt
     1741 gcaactgttt cgtcagttgt tccatctaga acagtcaata cggcaatacg tccgcccata
     1801 tgcaagtatc caccaaatgc agcattgtcg tctttttcta caacttcaaa gcgacggaag
     1861 ctgatttttt ctccgataac ttgagttgct tcgatcaagt catattcaat tgtaccttta
     1921 tctgttttga ttttcattgc agcttccatg tctgctggtt tgttttcagc aacaagttct
     1981 gcaatttctt ttactaaatc ttggaacatt tcgtttttag aaacaaagtc tgtttctgag
     2041 tttacttcaa cgattgctgc agtgttacct ttcacagcaa ctgaagccaa tccttcagct
     2101 gcaatgcggt catttttctt cgcagccttt gccattcctt tttcacgtaa aagatcaact
     2161 gctttttcca tgtcgccttc tacttcaact aatgcttttt tcgcatccat cattcctacg
     2221 ccagtcattt cgcgtagttc ttttaccatt ttagctgtaa cgtctgccat gtttaactcc
     2281 tcctgtgtgt ttttcttttt aaaaaaagct gcttcaaagg agagtgaggc ttgtcgccca
     2341 gcctttgaaa cagctccatc attcaaaatt attctgctga agaattgtcg ccttcaacaa
     2401 catctacgat ttcttcgatt gatgttgctg tgttttcttc tgtgaaatct tcttcaacta
     2461 cttgatcttc accttggttt ccttcgatga aagcatcagc catttttgaa gtaatcaatt
     2521 taacggcacg aattgcatcg tcgtttgaag ggattactac gtcgatttca tctggatcgc
     2581 agtttgtatc aaccatagct acgattggga tatttaattt gtgtgcttct tgaacagcaa
     2641 tacgttcttt acgtgggtca acgatgtaca ttacatctgg gattcttggc atatcagcga
     2701 taccgcctaa gaatttttca agacgttcac gttctttgtt caaaccagca acttcttttt
     2761 taggtagaac ttcaaaagtt ccatcttctt ccattgcatt gatttgtttc aaacggctga
     2821 tacgtttttg gatagtatcc cagtttgtca atgttccgcc taaccaacga tggttaacga
     2881 agtattgtcc tgcacgagtt gcttcttctt tgattgcttc ttgtgcttgt ttttttgtac
     2941 ctacgaataa tgctacgccg ccttcttcag caacgctctt catgtaatca taagctgcat
     3001 ctactaattt aactgttttt tgcaagtcaa tgatgtagat tccgtttctt tctgtgaaga
     3061 tatatttctt catttttgga ttccagcgac gagtttggtg accaaagtgt acgccggctt
     3121 ctagtaattg tttcatagaa attactgcca tgtttgtttc ctccgatttg gttttatttt
     3181 tcctcttctt atcttctacc ttgccggcaa actttttcaa gcacctaccg acaatccaat
     3241 aagaatgtgg atttgctgat gcataaagca ccttcagcta ttatacagac aaaagatccg
     3301 tatcgcaaga gcaaatatac ttttctgctc aacattcaat agtttacctg aaagataaaa
     3361 agaattcaag aaaaacagta gatttctctt tgtttcttta ttatcctttt tccataaaat
     3421 aattttaggt ataaaagacg aatattattg ggcaatcctt cttttttttg ttatgataca
     3481 tgtgaaatct ttcgtaaagg agaaagaaaa tgcgcaaagc tcaaagacat ttgctgatta
     3541 aacaagtcat tgaagaattc ccgatccgta cgcaagaaga actattaaac aaacttgggc
     3601 attatggcgt taccgccacg caggcaacga tttctcgaga tatccgtgat ctaaaaatcg
     3661 tgaaggcacc tgatgaaaat ggcgtttccc gcttcgtatt gtttcgcgaa aaaggaactc
     3721 ctgagactag gactgaggaa gaggatcggc tggttcaaat gattgaagac attgtattga
     3781 aagtcgaacg agtccacttt ttgacgatcg tcaatacctt gccagacaat gctcatttat
     3841 ttgcatctgt tttagatgag atcaaacctc cctctattgt cagtacaatt gctggattcg
     3901 atacgatcat ggtcatctcg gaaaatgagg aggacgcaaa acgagtagaa gaatttcttc
     3961 accatccaac tgaagtatca cttttttaat cagagcacgt attcctaata tggaatgcgt
     4021 gtttttttat tttcaatatt ttccttatta aataaacctt tacactcttt acaaaaatac
     4081 atcatttttt attcaatttt ttcaacacct tgattgaatc acaataatca tcatttatat
     4141 tggatgttaa caagagcagc ttcttgctct ttgtgtattt caaaaaagaa atgaaaggaa
     4201 atcatatgta taaattatta actcctggac cattgacgac ttctgaaaca gtgaaaaatg
     4261 ctatgctgat cgatcattgt acttgggatc aagaatacaa agaaattaca caatggataa
     4321 gaaaaaaatt attggatctt gctcaagtat cggaaggaat atacacaacg gtcttaatgc
     4381 aaggtagcgg ctcctttggg gtagaatcgg tattaagttc tgtgatcagg ccagaagata
     4441 ctgtactgat ttgttcaaac ggcgcatatg gacaacgaat gatcaacatg tgcgaatgtc
     4501 tgaaactgaa ctataccgtt tatgaggtat cgtaacatca aatcctccaa gctcatgtga
     4561 ttacacagct gattgaagag aatccctcta tctccgcact agcaatggtc catagcgaga
     4621 ctacttccgg tattttgaat aatttggaag agattgctgc tgtcacacaa gaaaaagatc
     4681 ttttgtttat agtggacgcg atgtcaagct ttggtgggat accgattcgt gtagccgaac
     4741 tagggattga ttttttgatt tccagctcaa acaaatgtat tcaaggtgtt ccaggctttt
     4801 cttttgtact tgctaataaa gcggaactag aaaagcgaaa aaatgtgagt cgaagtttgt
     4861 cacttgatct atatgaccag taccgcgtga tggaaaacga gaatggcaaa tggcgtttca
     4921 cttcccctac ccatacggtt ttggcttttc aacaagcgct gaaggaacta gaggcagaag
     4981 gaggaatcga aactcgatat tcacgttatc tcgccaataa tcatgtacta cgacaaaaaa
     5041 tgacagagat ggggttccag cctttcattc aagaatatca ggggccattc atcacctctt
     5101 ttctttaccc taaggactgg cgatttgatt ttaatcattt ttatcagtat ttaaagcaaa
     5161 acggatttgt gatctatccc gggaaaatca gcgatcatga atgttttcgt atcggaaata
     5221 ttggcgagat ctatccggaa gatattttgg aactgacagc aattatcaaa gactataagg
     5281 agaacgttaa tgattaaaac tgtaattttt gattgggcag gaacaactgt ggactttggc
     5341 tgtatggctc ctgtccacgc ttttagaaat gcttttctag aaaaaggaat ccaactgact
     5401 gacaaagaaa tccgcgaacc gatgggaaaa ttaaaatggg atcatatcca acagcttctt
     5461 tccctccctt ctgtcaaaaa acaatggata gctatttatg gacaactacc tcaggaaaaa
     5521 gaggtggagg aattgtatca acaatttgaa cattatctgt ttaaagattt ggtcaagcat
     5581 tctaccttga aacccgacac attggaaacg atcgaacggt tgagaaacgc tgggatcaat
     5641 attggttcca cgactggatt tactgctcct atgatgacaa tcgtagcaga aaccgcaaaa
     5701 aaagaagggt attcaccaga cttcatggca acttctgaag acgtcgaagg gtatggtcga
     5761 ccgtttcctt atatgatcca taaaaacatg cagtacttca aaaacaagtc ggttgatgaa
     5821 gtaatcaaag tcggagatac ggtctcagat atccaggaag ggaaaaatgc cggagtgttg
     5881 acagtaggtg taattgaagg tagttcgcta atggggattt cttatgatga gtaccaaaaa
     5941 ttagagatgg ggaaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagct
     6001 ggcgctgata tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac
     6061 aagaatccag aatgactttt atacaaaatc agaggtcgtg aaaaagctgt actgcatcaa
     6121 gtaataagaa cagccaaaca aaaatagctc ctcatatttt tcgtttggtt aggcttatta
     6181 ccgcagatgc aagcttttga acaccgttta ttcgaaaaaa aaagggagct gtgacacgac
     6241 ttttgtcaca gtccccttat ttacaaacaa acagtgttta agagacaacc tctccactaa
     6301 acggtgttca ttcagctgac tctcggtatt tgctcaaaaa acgataaaat ttgaaaagct
     6361 attttctctt ttgtttctct aatactcgga tcagaacgct gatttcacat tctctttttc
     6421 ttacatctta tctggtgcat gaagtcctag caaacgaagg tcttctttca ataatacagt
     6481 cactgcataa actagagcta agcgtgcttc tttttgttca tcatcagcta aaatcttcgt
     6541 atgagcatag tatttgttga atgcttgtgc taatttgatt gcatgtttcg caatcactga
     6601 cggttcgtat ttttccccag cagacaagac cgtttcaggg tatttttgaa ctaatttaac
     6661 cacttcccag ctggtatcgt cattcaatgc atagtctgct gtttctgaag ggacaaatcc
     6721 tgctttttcc aacaggctga ctgcacgagc atgtgtatat tgcacataag gtccagtttc
     6781 tccttcaaat cgaacaactt cttccaggtt aaagtcaaat gtattcagtc gatcgttttt
     6841 caaatcgtgg aaaatgactg ccccaacccc aacttgttta gctacagcat ctttgttttc
     6901 caaatcaggg tttttctcgc tgatttgttc tttagcagat tggattgctt catttaatac
     6961 ttcttctaat aagacgatct ttcctttacg agttgataat tttttaccac cttgcgtgat
     7021 caaaccaaat ggaatatggc gcatatcgtc agaccagtca aagcccattt ccttcaagac
     7081 agctttcaac tgtttaaaat gatagctttg ttcattccct actacatata gagattgttt
     7141 gaagtcgtaa gtacgtttac ggtacaatgc agcagctaag tcacgtgtaa tgtatagtgt
     7201 cgcaccgtct gacttcttaa tcaacgctgg attcaggtca taagcagata ggtcaacgat
     7261 ctctgcacct ttatcctcgt tcagcaaatg tttttcttct aaaagtttga cgatttcatc
     7321 catcttgtca ttgtagaaag cttcaccgtt caacgaatcg aaacgtactt ccagcagatc
     7381 atagatttta ttgaattctt tcatcgattc atcacggaac cattgccata attgaatagc
     7441 ctcttcatcg ccttcttcca gacgtttgaa ccaagcacga gcttcttcat ttaattctgg
     7501 ttctgtttca gctacttcat ggaattggac atataaacga agcaattcat tgattggttc
     7561 tgctttaaca gcttcttctg taccccattt tttgtaagcg acgatcagtt ttccaaactg
     7621 tgttccccag tcacctaaat ggttgatgcg gattggctga taaccgattt tttccataat
     7681 gaaaccgatc gaattaccga taactgtcga acgcaaatgt cccattgaga ttggtttggc
     7741 aatattagga gaagacatgt cgattggtac tgttccttga ttcccgatat tgctatcccc
     7801 ataatgttct ttttctttca ctactgtttg caaaactttt ttgctgataa gttctttatt
     7861 catgaaaaag ttcaagtaag gtccgacaac ttcgattttt tcaaagttgg cagaatcgat
     7921 tttttctgct aaatctgcag cgatttgctg tggcgctttt cggtaaactt ttgctaatga
     7981 aaaggctgga aatgcaacat ctccgtgttc agctgatttt ggattttcta gtaattgttc
     8041 gacttgttcc agtgttaaat catctttgac agcgtcatag acagcttttg cgacaagatc
     8101 tttattattc atctttattc ctcctaaagt gctcccttga ttgttaaaca aaaaaaatct
     8161 ctagcgacgg catacttagt atgccatcac tagagacgag atttcccgcg gtaccactcc
     8221 aattgtccta aaaaaggacc agctccatct gatatcggta gacgaccgtc tgtttaaaac
     8281 agaattctcc aagtgcgctt cgttgtatct tcttcattcg gcttccacca cttccgaatt
     8341 cgctagccaa gaatgataca agtactctct tgttcaacaa atcttagtct tattaaactt
     8401 tcttgaatta tatcagaaaa aaaaacgaaa ggctagtcgt ttctgataaa aaaacaatcc
     8461 agatggccta aaggagaaga aataccacct ggatagctat aaatcatttc gtaaatacga
     8521 aagacttaac aaattaatca gctacgataa tcgttccttc ttcagaagct aaaagattct
     8581 cgatattttc taaagaagtg atgattgcct tagcgtttgg ttttgcttca acaaatgcaa
     8641 tagctgcctc tactttcgga agcatgcttc ctggagcaaa ttgcttttca tcaatgtatt
     8701 gtttcatttc tgaaacagta acagtttcta atttcttttg atctggtttt tggtaattga
     8761 catacacatt gtctactcct gtcaatacga tcagcaaatc cgcatcgatg atttcggcta
     8821 atttttctga agcaaaatct ttatcgatta cggcttctcg tccttctaat ccagtagctg
     8881 tttctacaac aggaatccca ccgccaccaa cagagactgt gataactcct tgatcgacca
     8941 gcgtttcgat gacacgtgct tctttaatcg agatgggttt aggggaagcg actacttttc
     9001 tccatccacg tccagcatct tctttgaatg tcacagtgga gtcagcattc atttgttctt
     9061 tggcttcttc ttccgtgtag aatggaccga ctggtttact tggatttttg aatgaaggat
     9121 cattttcatc cacaataact tgagtgacta aagaaaccac atctttatcg atcccttttt
     9181 ccttcagtac ttcgcccatg gcattttgta accagtaacc gatagatcct tctgtcattg
     9241 caacacatgt atctaaaggc attgccggtg ttttctcaga atcagcagct tgctgctgaa
     9301 tcaataggtt accgacttgc ggaccatttc cgtgggagat gatcaattca tctccttgtt
     9361 cgataaattt cactaaatac tttgctgttt ccattaacgc ttcttgttgg gcttttgcac
     9421 ttgcatccgt agacaagatt gcattacccc ctaaagcgac aactactttt cgtttggcca
     9481 tttcattcgt cctttcttca gttctaccga attttctaga taacaaaacg agccagcgtt
     9541 ttgtgatttt ccctcaatgc tgactcgttt tttcgtgaga tgaatgtggt tatgcttaaa
     9601 cgcgagggat aaacaagtta cctaatgttg ctgccatgat cgctttgatt gagtgcatac
     9661 gattttctgc ttggtcaaat tgacgagcat atttgctgcg gaagacttca tctgttactt
     9721 ccatttcagt gatgccaaag cgttctttca tttgttcacc gtaaacagtg tttgtatcat
     9781 ggaaagctgg caagcagtgc aagaagatca aacgatctgt gttgtgtgtt ttttctacca
     9841 tttccatatt gatttggtaa ggtttcaaca atttgatacg ttcttcgaat ttatcttctt
     9901 cacccattga tacccataca tcagaataaa ggacgtctgc tccgtctaca cctttatcaa
     9961 catcgtcggt aatcatcaat tgtgcaccgg atttttcagc aaagccttct gccattttga
    10021 cgatttcttc ttcaggttgt aattcttttg gtgcaacgat acgcatattc gctcctaaaa
    10081 ttgcacctgt gactaataaa gagttagcca tattgtttcg accatctccg cagtaagcaa
    10141 cagtgatacc ttctactgtt ccaaagtttt cttggatagt taaaaagtca gcgatcattt
    10201 gtgttggatg ccattcatca gtcaagccat tccaaactgg aacaccagag aattctgcta
    10261 gttcctctac cattttttga ctgaatccac ggaattcgat tccatcaaac atacgaccta
    10321 gtactttagc tgtatcttct gtggattctt ttttacctaa ttggatatca tttgcaccta
    10381 gatattcagg atgtgcacct agatcgatcg ctgcagtagt aaatgcagaa cgagtacgtg
    10441 tagatgtttt ttcaaataaa agcgcaatat tttttccttc tagatagtga tgtgggatac
    10501 cacgtttttt caagtctttc aagtgttctg aaaaatcgat caaatattgt aattcttctt
    10561 ttgtaaagtc tttttctgct aataagcttc ttccttggaa tactgattct ttcatattaa
    10621 acattctcct ttagattgtt tttatttaag attaaagagg aaatcccgac ggcagctgac
    10681 agtaagaccg ccgtcaggaa acaaattact tcaagtcttc acggacaagt ggttggctca
    10741 tgcagcgtgg accaccacgg cctcgtgaca attcacttga attgatttct aacactttga
    10801 tgccatagct acgaagcaat tcgttcgata cataatttcg gttataagtg actacaacac
    10861 ctggagcgat tgccaatgtg tttgatccat cattccattg ttctcgaggt gcaacgatct
    10921 catctccatt accagttggg atcagtacaa gatcatctaa tccaagagca gctttcatcg
    10981 tttcgtgtaa gttatctgaa tgagtaatct tgatatcatc accatctggt tcgattgtaa
    11041 agacatccat cttaccgtct ttactttgaa ttgctggatg gatagtgaat ttatcgtgat
    11101 ctaccattgt aaatacggta tccaaatgca tcattgcacg gttgtttgga attttgattg
    11161 ctaacacttt ttcaaatcct gaattttttg cgaataggtg gcgtgctaat ttttctaaag
    11221 ctttcgcatt agttcgttga gaaatcccaa cagctactac tttatcgctt aagatcaatt
    11281 cgtctccgcc ttcgatatga tctggatttt cacgatccaa ccatacttcg attcctttat
    11341 ttgcaaatct tggatgatgt ttcaaaatat attctgtaaa catcgattca cgacgacgtg
    11401 cttcaaaagt catcttattg actgtcatcc catttcccat tgatgcggaa gggtcgcgtg
    11461 taaagtacaa gtttggcatt gggtccatgt aaaatggata gtcatcgtct gctgacagat
    11521 cgtatagact gctagaacgt acttcaatat cttttgtacg aaccccagcc atgattttat
    11581 ctaccatttc ttgcgtttcc attgatagaa ggaactcatg aagaccctct cttacagcat
    11641 tcgatgcaat atgagattca tccaacattt tattcaagaa ttgttctttg acattgcctg
    11701 cgtcaatcgc ttctgccgct aatttttcca agtaaagagt ctcgacgcct tcattttgca
    11761 atgtctttgc aaagttgtca tgttcttctt gagcaatcgg aagatatgga atatcatcga
    11821 acaacagacg atccataata tctggtgtca aattttccac ttcttgtcca ggccttttca
    11881 gtaaaactgt ctttaatttc cctatttcag agaaaacgtg aataggttta tccatgttgt
    11941 gttgcctcct tctttttgat tacatgtata ggatatcctc cttttgtaat cactttcata
    12001 attttgtctc attcaaaatg agacattcct cacattaata ttcgataatt aaataattaa
    12061 acgtttttct atttttaagc g
//
LOCUS       DAYYME010000086        11908 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000086 DAYYME010000000
VERSION     DAYYME010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.087"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..465
                     /locus_tag="ACHBFW_001935"
     CDS             <1..465
                     /locus_tag="ACHBFW_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFM9318237.1"
                     /translation="WKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQAAYAF
                     LKRLVKQFDEPKVVVTDKAPSIASAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVK
                     RRNKFYRSLRTASPTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPD"
     gene            complement(1080..2120)
                     /locus_tag="ACHBFW_001936"
     CDS             complement(1080..2120)
                     /locus_tag="ACHBFW_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM9318238.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2469..2795)
                     /locus_tag="ACHBFW_001937"
     CDS             complement(2469..2795)
                     /locus_tag="ACHBFW_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318239.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2782..3570)
                     /locus_tag="ACHBFW_001938"
     CDS             complement(2782..3570)
                     /locus_tag="ACHBFW_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9318240.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3938..4228)
                     /locus_tag="ACHBFW_001939"
     CDS             complement(3938..4228)
                     /locus_tag="ACHBFW_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM9318241.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(4364..4543)
                     /locus_tag="ACHBFW_001940"
     CDS             complement(4364..4543)
                     /locus_tag="ACHBFW_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318242.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4540..4890)
                     /locus_tag="ACHBFW_001941"
     CDS             complement(4540..4890)
                     /locus_tag="ACHBFW_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318243.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4883..6208)
                     /locus_tag="ACHBFW_001942"
     CDS             complement(4883..6208)
                     /locus_tag="ACHBFW_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM9318244.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6658..7272
                     /locus_tag="ACHBFW_001943"
     CDS             6658..7272
                     /locus_tag="ACHBFW_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9318245.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(7437..7679)
                     /locus_tag="ACHBFW_001944"
                     /pseudo
     CDS             complement(7437..7679)
                     /locus_tag="ACHBFW_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7883..9202)
                     /locus_tag="ACHBFW_001945"
     CDS             complement(7883..9202)
                     /locus_tag="ACHBFW_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFM9318246.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(9336..9944)
                     /locus_tag="ACHBFW_001946"
     CDS             complement(9336..9944)
                     /locus_tag="ACHBFW_001946"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318247.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSLNNSYF"
     gene            complement(10582..10839)
                     /locus_tag="ACHBFW_001947"
     CDS             complement(10582..10839)
                     /locus_tag="ACHBFW_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM9318248.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(10832..11101)
                     /locus_tag="ACHBFW_001948"
     CDS             complement(10832..11101)
                     /locus_tag="ACHBFW_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM9318249.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(11232..11411)
                     /locus_tag="ACHBFW_001949"
     CDS             complement(11232..11411)
                     /locus_tag="ACHBFW_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318250.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            11509..11745
                     /locus_tag="ACHBFW_001950"
     CDS             11509..11745
                     /locus_tag="ACHBFW_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318251.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
BASE COUNT         3675 a         2151 c         1826 g         4256 t
ORIGIN      
        1 tggaaaatgg atgaaacgta catcaaaatt aaaggaaaat ggcattattt gtatcgagcc
       61 atcgatgcag atggtttaac cttggatatt tggttacgta aaaaacggga cacacaagca
      121 gcctatgctt ttcttaagcg gttagtgaag cagtttgatg aaccgaaggt tgtagtcaca
      181 gataaagcac catcaattgc tagtgccttt aagaaactaa aagaatacgg attttatcaa
      241 gggacagaac atcgtaccat taaatacctg aataatttga ttgaacaaga ccatcgtcca
      301 gtaaagagac gcaataaatt ctatcgaagt ttacgcactg cctcacccac gattaaaggc
      361 atggaagcca tccgaggatt atataagaaa acccgaaaag aaggcactct cttcgggttt
      421 tcggtctgta ctgaaatcaa ggtattattg ggaatcccag attaaatcat agataccgta
      481 agggatttta ttctttattt aaaactttgc aacagaaccg tccgttgcgg gtaataccgt
      541 aacactgaag ggcacgcatt aaggcgtcat cttaatgctt tcctgtagga cactgacgga
      601 gattaccagt ctcccgagcc ttacaggggc ctttcttttt ttgtattttc aattatcaca
      661 atttatcgtc caaaagtgca gaacgaaaat caattcatca caagcatatc agacaattaa
      721 atttaaaaca actaaaaata tacaaaaaag tgtaattgaa tattagcagc actgcctgaa
      781 aagaagtaat atcaggcttt tcagcttggg gaaataagtg aattacctct cttcatgaat
      841 tattataact caaaaaagca catatgcaaa cactagttcc ctattttttt gaatgaaaaa
      901 taactaaaat atctaacgat tatgacttta aaacctacgt ttgcgattgt tttcataaac
      961 ttttaatcta aaaactccgg aataatagct atttatttgt aacatttgta ataaaattaa
     1021 aatcataaaa aagttctaat ttaaaattga tcatttttat agtgatcgct ttttatcaac
     1081 tactctagcc aattttccgt actaactgtt ggtaattcgt taaatgtttc tctttgttcg
     1141 tttgcaagct ttgccgcaat agctttttcg acaaaccaat tttttacata aacgaaaatt
     1201 agattctgca aattgtttac tttttcagtt ttttgttttt gataagcttt cagcaaagtc
     1261 atatataatc ctttgtcagc atcaattcca taagattcca gttcttcata aacaacaact
     1321 gtttgctcta attcttcagc ttttttcttt gcattatgaa tagtttggac ggttttttga
     1381 gcgtcttcgt aagtactaga aaatgttttt atcaagttta atacattttc tggtacaaat
     1441 gtatttatac tgtcctcttt catgatgtcg acgaagttat ctagtaaaac ttgattttgt
     1501 aactgatcag tttcagtgtc tattatgtgt ctattagtgt ctatagaatt agaatagaat
     1561 agattgtctg cgattttcgc aattacggtt gagtccttga tatgactgga ttttgccgta
     1621 tttgcgattt tcgcaattac ggtggtatca ttgatttctc gcggttttgc cgtatttgcg
     1681 attttcgcaa ttacggtggt atcagtattg tcttcctctt ccatgatttt aaagacgtct
     1741 tgattcgtta cttctggctt gcctaagtac aataaattag gcatggattc aatcttaccg
     1801 tcacgtttat ttactcttcc tctcttctgt ttcagaagac ctgctttttc taattccttt
     1861 ttgattttag atactttacc ttcgcggcat tgtaacaatt tcattaattc ttcaactgtg
     1921 aatatgaaaa atatattatc atcagaatct acccagttat ttttgactga ataatcaaat
     1981 cgatctttaa gtagcatata agcaattttt gcatcatttg atagatccat gtatttttcg
     2041 ttagtaaaaa ataccttagg catttgataa tatttttcac gataaatttc ttttgatttg
     2101 taataattaa aatcattcat agccaatcct ctttctgtag gatagcgttt tgttcaaaac
     2161 tatgttacaa tgaattaaac aaatatatct atccactata tatttttcta ctgctgataa
     2221 gtcgccaaac tttgttaaag cagtagtctt tttttatctt tttttagttt accttaaaaa
     2281 ataaattaaa gcaaaccgct ctatttcaag gtttaagctc atagtaacta tatcacgata
     2341 ttacaataat attatatcac gatatcgtaa tattacaata tgaagataag cgacttatca
     2401 gctttgatac tactgatttt tataaaaaaa atatcacgat attatgatat cgtgatattg
     2461 aatgtaaatt aaattttatt ttgtttcttt gctagtttat atccttcaat caagtattct
     2521 tcaggtatgg actttaatag cgcatcttct ataacgtctg gtttactttt gtctgattca
     2581 gcaatagtcg tccaatattt gagtgctgac attagaattg gatttatacg aatgctatct
     2641 gctgttctta cactacttcc ttgatctttt atgactttac ttatattttt cttggttaat
     2701 tcgatttctt ctacttcttt tgatttaaaa ttctttttat ttttatttcc tagtaatcta
     2761 cttactgcag cttgactgcg attatcttct gccatcaaaa tcaccaactt cttttaaata
     2821 ttcttctgta aatgctttat agtcatcttc agctgttgag ggtttattaa aattgattag
     2881 atctgatcca gcagcttgcg cttcgtcaac ggttattgtc ctacgaatat aagttttgaa
     2941 aatttttgca ccaatgactc tagaaatttc ggttgctgca gacgtcattt ctttagaaat
     3001 gttataacgc ttattatact tagtaaatag aactcctcgt acttcaagct tgtcgttcgt
     3061 atattctttt accgtttcaa tagtttcact taactttatt agtcctttta atgagaaata
     3121 cgcagcttca actggaatga taatttcatc agaaacagta aatgcgttga ctgtaagcat
     3181 tcctaaactt ggtggacaat caattataat ataatcatag ttgtccttga ttggttgaag
     3241 tactctttta agcctgtgtt cacttcctac ttgagtaaat tccctctctg cagaagaaag
     3301 ggctatgtcg gctggcaaaa catcaattcc tttatagtcc agaatagcat ctgtaatgtc
     3361 agcctcccct tttaatactt cataaattgt aggttggttt gttccatcta tatcgaaacc
     3421 tacattatct gtagcatttc cctgaggatc aatgtccact aaaagtacat ttttaccgta
     3481 aacaaatttc aaacatgctg ctaaattaac gctagtcgta gttttagaaa ctccaccttt
     3541 ttgattggct actgacaata ttcttgccat taaaatatcc cccattcatc ttatattata
     3601 atataattat atcataatat tgtgatattg caatatcgta tatgcattat attgtaatat
     3661 tgtgtgatta ttttgtttta aagatatata tcggttcgga tataaagatt attcatatgt
     3721 tattcgaaat taaaataatc actttttatt actttaaaat tttttattaa tgataagaaa
     3781 tatgttttgc gagcagcaac tagaataatt taaacattac taaactgtct atattgttac
     3841 taataattcc cttcttatag tgcattttct tagaaaaaaa caatattacg atatcatgat
     3901 attaagatat cgtaatatta tacaattgtg attgatatta tatatgtttt tttatttttt
     3961 tatcatgttt aatcttgaag gaatcatatt ctttttctaa agacaggagc ttttcataaa
     4021 catcagacat gtttaaacta gaggccaggc ttaaatcata aacgaatgaa gcaggtaatg
     4081 attttattct acgttgtcgg gttatccagc ttgcaaccgt tccttgtggg taggcatata
     4141 actcacaaaa ttgttctaca gtgattccta aattcttgat aatgtaaaca ttgatcgggt
     4201 gcgagtaaac aaatgtattg atagacatgg tgcgtaagcc tactttcttt tattattgcg
     4261 tttgcaataa tagtttaaca cacgcattat tctagtatct actcctattt gtaaaaacaa
     4321 aaaaggattc atagagataa ctctatagaa tccgtaaaat accttatata tataacggga
     4381 agatataaaa cctaaaatta tgataacctg ctgcagcttc tacatagaaa gagaagcgct
     4441 caacaccaac ctcatttatc aaacgttggc cataacccca aatctcttct gatagctgat
     4501 caaaggttat atactttagc tcatattgct ggataatgtt cattctaatc gactccattt
     4561 attatcctgg taaaattcga tatgacgtat ttcgtcgtag tgaattttgt cttttccaac
     4621 gtagatcccc agctcatcac tacctaagat cttgccaatg atatcttctg gatagtatcc
     4681 ttcagcgttt cgttcttctt tttgaataga tactgatcta ttttttatga ctgcagcgtt
     4741 caatacttcc gcaatctctt cagtcgtcat ttgttctttt gctaaatttt gactggttcg
     4801 ttgttcattg tcggcatcaa tttcttcagt atgatcagat aaataaaacc ctgcccactt
     4861 taaacgtcca cgatcattgt aatcaatcat ttttaattcc cccatttccg cccgcatgac
     4921 caccaacaag tgtgcttcta gtaatgcttc tagcgccttc taacattgaa gaagcatgta
     4981 caatcgacgt aaaaccatat ttttgccgaa tcttgtcaat aattttatct aaattttctt
     5041 cgtcaatttg cttttgtgga tcactaaata aatccagctg aagcgaatcg gtgtacacaa
     5101 gcttgctata ggttattcca acatgcctta cttcttgtcc ttcataatac ttatcaaaca
     5161 agaaaagaca ataattctgc aattcctttg tattatctgt gatagggatc ttcatctggt
     5221 gtgaaaaccc tggtctcgtt tctaagatcg acgtgccaat gtgtaaatgg acacaacctg
     5281 ttttacagtt atgctgccta atgcgtatcg ctacttgttc cgccatttct tttaccacca
     5341 gctcgatttc atttcttcta gtataatctc gtggtaacac ttgtgaattt ccatatgatt
     5401 tttcctttgt cggttctggt gggattgcta tatctgttcg atcgatccca ttagcatgga
     5461 agtacagctg taagccaatc acacctagtc tattcttaat cgtataagga ttccagttag
     5521 caagatctct aatgctcata atccccatct gattgagtct ttttttcatt cgagagccaa
     5581 ttccccaaaa gtccgtcatt tcaggaatat tccatacttt ttcaggaacg ttttcgtacg
     5641 tccattccgc aacaaatcct tcattatgct ttgcttcgtt gtctaaggct agtttagcca
     5701 aaaggggatt atctccgaca cctactgtag caatcaatcc tagctcttct ttaatattct
     5761 cctggatcat ttgagccagc ttctttctcc gttgacttcg tgttccttca gtcgtaaaaa
     5821 gattcagtga tcgggtcact tttaagattg attcatcgat cgagtaaatc agtagatctt
     5881 catcagccac atatcttctg aaaatattat ttacctgcat atttcgcttg atatataggt
     5941 tcatacgtgg tggaacaacg tgtagtgttt taggaaatgg ttgtggtaag tcacgtggtc
     6001 tactcacatt tgtaatacca taccgctttt ttgcttcagg agaagaagct aatatcaatc
     6061 ctgaaccagt attatcagct cgactcataa caacaagttc tgttgttaat ggatctaaat
     6121 ttctttctat acattcgaca cttgcgtaaa agcttttcat atcgattaaa aagtaatcat
     6181 ttactggctc ttttgaataa tccatcataa ataacactcc attctttcac attatacaaa
     6241 catatgttcg atattgtaaa gaagaaatag ctaaatacat aaaaaaagaa gcataggtat
     6301 gctatcaccc tgcttcttct tttgatttgt atgatcaatt tattctatca ctttactaat
     6361 taatttgaaa atctaataaa aactctcact ctaaaagagt gagagactgc aaattagagt
     6421 acggactttt ttggagttcc gttctttaat attaacaaat gaataaaatc cttacaacat
     6481 ttgagaataa gttatcagta gacacttaca aaaaattctc atactatttc ctccatcatt
     6541 agtttttgtc tatataaggt ttaccctaaa tagacactgg tataacattg aaaaatatgg
     6601 cttcctcgtg tatgattagg gtgtatttta aatagttgca gaaagtaggt aaaagttttg
     6661 agagttgcat acatacgtgt ctcatcgatc gatcaaaatg aacaacgcca gattgaagaa
     6721 atgaaaaaat tcggtgcaga gagaattttc atcgagaaac aatctggcgc aacgattact
     6781 catcgacctg ttttccaaga agcattagat tacgtccgag atcaagatat ttttattgtg
     6841 gaagctattg atcgacttgg aagaaattat gacgagatca tcgcttcagt caattacttg
     6901 aagaagaaaa atgtgaagct gatcattact agtttaccga tcatggctga agcaattggc
     6961 aatcctctcc tggataaatt cattaaggat ctgatcatcc aaattttggc catgatcgct
     7021 gaacaagaac ggactgaatc gaaacgtcgc caggcacaag gaattaagat cgcaaaagca
     7081 aacggcgtct acaaagggcg gcccaaatta tacagtgcag acgcaaaaga tccccaacgt
     7141 cgccttgttt acaaaagcat cgttgaagat cttaaaaatg gtgttgcgat tgctaagatt
     7201 gctaaagact acaatgttac gagacaaacc gtgtatagaa taaaaaaaga cagcatggtc
     7261 aatgacaaat agttgatcat gctgctttta ttatcttctc atctttaatt tcctacatat
     7321 aattactatc tataaaatta agtcttactc agaacagata aagcataaaa ttccaacaaa
     7381 ccatttttta cttaactaaa taaagagtct gttaatataa tcttcactta tttacgttaa
     7441 tcccctactg tatttgttgg ttccttatct atattttcta caaaatcctt attatcttgt
     7501 gatttagctt cgtataattc tttgtatgtc gacacattgg ttatactaat tcctatagct
     7561 agttcttgag actctacttt tctctctagc tcctttgaca aagtgatttg agatttattt
     7621 ggttccgttg gagtcaattc tatagattct tcataaaaat ctctatacat aaagctatcc
     7681 ctgaataatt catagcttta attctttggc acattttatt gaaatatgta tcactaatat
     7741 ccctatcaac tgattttgac taaaaagtta cttcaaacat tcactgttca aaaatattga
     7801 ggaataaatt ttgggtgtaa ggcattttga gcatacttct ccctggataa cttcccagta
     7861 aaaaaatcgt tagattgttg gattagatcc attgactggc tcgcctcagg cgagcaaaga
     7921 ccctgtagaa ttcattctga tacacatgat aactagataa tttgagatag actcgtctac
     7981 ccgtttggac taatttccca gcaactttca gaaacttcaa tcgaatagaa tgaatggtca
     8041 ttcccttttg gacttcttca aagccaatcg ttcttaagaa gttgactaag ttgtaagcta
     8101 tcaagctgag catcattcgg acatgattct ccaaaaagcg aggactgtct gttttgtcaa
     8161 agtaaaagcc agctttcgct tctttaatga agttctccat tgtgccacgt ttggcataga
     8221 tagaaaagat ggtatcagga gaaacatttt ctgatagatt cgtcacgata aattcatgtc
     8281 gaaagagtag ttcgcccgct tcacgtgttg agcgtataca cacgcgacga ctttgtgacc
     8341 aggattgtgc ttgataagtg gtcgagaagt actgaacttc tcgttcttcc cacttttgat
     8401 tatcgccata aagtactgat ttctcagcta tttgacctag tctacgatta ttcttcagtc
     8461 gaataacata atgactcttt tttgattcac acgaatcata cacaccaggt gtagcgaacc
     8521 cgctgtctcc acgaaccaag atgtcagtgt ttggtagaga gtgattatag tgctctaata
     8581 aaggtgtgag aaactccttt acgccttttg atgtatattg atttcctgaa cgtagttcag
     8641 cttttaagaa gtcaccagtc aatccgtcaa aagctaccaa tggatggtaa ccataggttt
     8701 ggtagtgtgt attataattc gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat
     8761 ctaaatcaat gattaattcc gtatcattac ggatgaggcg tgctttatcg ataagttctt
     8821 ggttcaaagc ttgaagttca tggatattct cctcagatag tcgatctaaa aatcgggaaa
     8881 gtgaagattg agaagcgagt tctttcctat ctaaaacagc tttaaacaca ggatcttgtc
     8941 tcaggagatt agctgcagaa tcagctgagt aaccagcaat taattgcata atgaactgct
     9001 caagtatgga taagttgtca tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt
     9061 gcttagccag ttcagaaaaa ccaaagatgt tcatcagctc tttcactagg acgagacccg
     9121 aatcactcga taattgacca cctgtatgag aaatagtgat atttgaattg aattttactt
     9181 ggtttttgtg taagctagtc atgaaaagaa ctcctttctt ttggttggtt tagtcacttt
     9241 aaccatagca gaaaggggtt cttttttcat cacttaacgg gtgaagatga aaaagtaatt
     9301 gtaagctgcc gtgaggcagt ttcacatggt tttaattaaa agtatgaatt attcaggcta
     9361 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     9421 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     9481 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     9541 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     9601 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     9661 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     9721 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     9781 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     9841 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     9901 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     9961 aaatcagcta atttttcttt agaataaaaa aaaacattta tatcagaatc aataaattct
    10021 atttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
    10081 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
    10141 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta
    10201 aattctatga gaagtttatt tttgttgttt ttgctaatta actaaaacta gtacaaaaaa
    10261 tttaaaatag attattaaac ctattgctta ttctattcag tcatatatat aagcgtcatc
    10321 ctttagtaac taacatccca cactacaata gtattataac atatctattc aacttcaaat
    10381 tgttgaaatt taagccattt atatttactt ttcctcaact ttagaaatga ttttactaac
    10441 cttatatccg taatacgcgt aacaaacaca ttcattaata acaatcaaca ctaagctata
    10501 ttagcttttt aaatgactag ggtaaataaa ataaggatca aaaactttta agtttctgac
    10561 cctttcctta cttccgattg gttaatagtg atcttttgca gaataaataa atatcgtttc
    10621 attttcaact ctatatatca gtctatgttc atctgtaatt cttctggacc attttccaga
    10681 taaatcatgc tttaatggct caggttttcc taatccagca aagggggaac gatcgatatc
    10741 ttttattaac ttgttaatct tttttatatt gcttttgttt ccttgctcat gccaataaag
    10801 ataatcatcc caagcatcat cagaccaagc cttaatcatc agattcaacc tcgattaagt
    10861 catgtgtttt aaatgcacct ttggagaatt gttcatctcc tcgacgaatt ttttccatga
    10921 cgtaattatt agaaagtgtt ctcaacgttt cttgcataga atcataatct ctttttgata
    10981 atacaacaac tgtatcttct acatctttac ttgttacaat aagtgtttca gcatcctcat
    11041 taacttgttt catataacta cgtaaatttt ggcggaaatt tgaataagct actgcttcca
    11101 ttcctttcac cccttattta tttctataaa acaattgtac attatattgt acaattaagc
    11161 aaatgattaa tattctaact tcttataatt aaaagtgcta tcacttttag tttttaggat
    11221 aaaaagtgac gtcacttttt gagtgctggt acctcaaatc ctgctttttc ttgggcaatc
    11281 aataaatcta acgcaataca cataatcctg gattcacttt tgccgtactt ctgctttagt
    11341 tgctgcaatt ctttttgtct cttttcatcc aacgtaacgg gtaaccgttt atttcctatt
    11401 gcaattggca ttagtactct ctcctttcat cgaaataacc ctatgaaaaa tatggctgca
    11461 atcatcttga atagtagact cacaagggct aatacaaagc gccaaaagat caaacaaaat
    11521 accagtattg ccaaaaatac tacgaagttt aaatgcacag ctgcagcatg gcgcataagt
    11581 atttcgccaa caaataggac taacaataga ggaatgattt tattcatatg gatctccttc
    11641 tttctcttag gttatcgggt gttgcccgct tatttcgcac cacctaagcg gcgggggcgg
    11701 ttggagcgat tggtgcgaaa taagaaccat tcccttagtt actaggtttt ttgtccttat
    11761 ttcacacatt ctcacttttc ggtataaatg attattgcct ttttttcctt tcaaatcgtt
    11821 gatatggcat tcgtttacgg tcaagttcat tttggatttc ttctgtatcg tcgtaagtat
    11881 aggtgtctaa aaaatcagtt aaagtcgt
//
LOCUS       DAYYME010000087        11904 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000087 DAYYME010000000
VERSION     DAYYME010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.088"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..2098
                     /locus_tag="ACHBFW_001951"
     CDS             80..2098
                     /locus_tag="ACHBFW_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9318252.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    2168..2374
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2525..4939
                     /gene="leuS"
                     /locus_tag="ACHBFW_001952"
     CDS             2525..4939
                     /gene="leuS"
                     /locus_tag="ACHBFW_001952"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFM9318253.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            5287..6840
                     /locus_tag="ACHBFW_001953"
     CDS             5287..6840
                     /locus_tag="ACHBFW_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFM9318254.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7221..9005)
                     /locus_tag="ACHBFW_001954"
     CDS             complement(7221..9005)
                     /locus_tag="ACHBFW_001954"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9318255.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(9020..10741)
                     /locus_tag="ACHBFW_001955"
     CDS             complement(9020..10741)
                     /locus_tag="ACHBFW_001955"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9318256.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            10841..11602
                     /locus_tag="ACHBFW_001956"
     CDS             10841..11602
                     /locus_tag="ACHBFW_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9318257.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         3894 a         2155 c         2416 g         3439 t
ORIGIN      
        1 gctcttaaat aacttttaat aaatattaag agaagttata attattaata atagataaat
       61 tgaagttgga ggatagaaga tggaaaacat tgcaagaaaa gaacggagac gtttagaaca
      121 gaccaaacgt tttcgaaaag caaaacgagg ggcagcaatc gtgggaactg cgatggtagg
      181 ctgttctgtc gctgccccat tagtacagcc ggttcctgtt caagcggatg aaacacctgt
      241 tcagttcggc gcaagaatca atacagcggc atttatcaca gaaattgcta cttatgctca
      301 accaatcgct caagccaacg atctatatgc atcagtgatg atcgcacaag cagttgtaga
      361 aagcggttgg ggcagtagtg cgttatctca agcaccttat tataatcttt ttgggataaa
      421 aggcagctat caaggacaaa ccgtctacat ggatacgctt gaatttttaa ataatcaatg
      481 ggtaaataaa aaggagcctt tccgacaata tccttctttt gctgaatctt tcaatgacaa
      541 tgcctatgta cttagaaata cttcatttgg caacggttat tactatgccg gtacttggaa
      601 gagcaacacg aaatcctata cagatgcgac agcttgtttg acagggcgtt atgcgactga
      661 tcctggttac gcaggcaaat taaataatat catcgcaact tacggattga cccaatatga
      721 tacaccagct agcggaaacg caggcggtgg agcgactaca ggtggcaata atactggaag
      781 tgcaggtagt actggcggtt caagcaattc tggtaataca aactcaggtt cagcaacttc
      841 gaccacttat actgtccaat ctggtgattc tgtttgggga atctcaaata aattcggtat
      901 cacaatggat caattgatcc aatggaacaa catccaaaac aatttcattt atccaggaca
      961 aaaactgaca atcaaaggta gccaggaaaa tggctcttcg acgaataatt ccggtaacaa
     1021 taccaattct tcaggaaatg ctggtacatc aaacggagga caaacaactg gtgcgaaata
     1081 cactgttcaa tctggtgatt ctgtttggaa gatctcaaat gatcacggaa ttacgatggc
     1141 tcaattgatt gaatggaaca atatcaagaa caatttcgtt tatccaggac aacagctaat
     1201 cgtatcaaat ggcggttctt ctaatgctgg agcaaatact ggctcaaata ctgggaatac
     1261 aagtggctct agttcgtcca gctcaagttc ttctaataca gcttcaggaa cgaaatatac
     1321 agttaaagcc ggagactctg tttggagcgt ctcaaataaa tacggaatta cgatggctca
     1381 gctaatccaa tggaacaata tccaaaataa ctttatctac ccaggacaac aactgactgt
     1441 ttcaaacagt ggatcaacaa acagcacgtc aggtaataca ggttcgtcaa ccaatacaaa
     1501 taacagcagt tcagttaata ctggcagcag ttcgtctcaa ggcacttata ccgtgaaagc
     1561 aggtgaatct gtttggagcg tggcaaataa aaacggcatc acgatggatc agttaatcga
     1621 atggaataat atcaaaaaca acttcattta tccaggacag caattgatcg ttaaaggtgg
     1681 aacttcggca aatacaaata caggttcaac tacttctgca gcgaaaccaa atacaccgaa
     1741 tacgtctgct acgacaagta catcatccac tggtaatacg atgtataccg tgaaagcggg
     1801 cgagtcagtt tggagcgtag cgaacaaaca tcatatcacg atggatcagt taatcgaatg
     1861 gaataatatc aaaaataatt tcatctatcc aggacagcaa gtgattgtga agaaaggttc
     1921 atctcaatca acaaaccaac aatcagttac tggttcgaaa acctatactg tgaaatctgg
     1981 agaatctgtt tggagtgttg ctgacagtca tggaattaca atggctcaac taatcgaatg
     2041 gaacaatatt aagaacaact ttatttatcc aggtcaaacg ttgattgtag ctaaataaaa
     2101 tagctgttcg aagaaataac gattgaattc cacaaaaata tttgttacta taaggctact
     2161 taaatatgac aacacttgaa aaggaagtag taaaaagact gaagtttcag agagcgcatg
     2221 gctggtgaga atgtgcacgg atgcttttga actcgccttt gagttgccct tgctgaatac
     2281 ccgagtaagc aagcggcggt gacggccgtt acaacgttcg aggccttttc actaaggtaa
     2341 aattaggtgg taccgcgtag attacgtcct ataaaaagag caatcttttt ataggatttt
     2401 tttatttctt ttgtgaagtt tgagaaaaca aagcaaaagg aaataaaaat aaacagattc
     2461 tgccggtttt ttcgttatat ttataataga aacaagtgtt ttcaatagaa caggaggaca
     2521 gcaaatgagt tacaatcata aagaaattga gaaaaaatgg caaaaatact gggcaaaaac
     2581 gaatactttc aacacccatg atgatcctga aaagccaaaa ttttacgcac tggatatgtt
     2641 tccctatcca tccggtcaag ggctacatgt agggcatcct gaaggttata cggcaacgga
     2701 tatcctttca cgtgtgaaaa gaagtcaagg atacaacgtg ctgcatccaa tggggtggga
     2761 tgcattcggt ctgcctgctg aacaatatgc gctagataca ggaaacgatc cagctgagtt
     2821 tacccaaaaa aatatcgaaa cattccgacg ccaaatcaat tctttaggat ttagttatga
     2881 ctggaatcgc gaagtgaata caactgatcc ggagtattat aaatggacac aatggatctt
     2941 tacgaagctt tatgaaaaag gtctagctta tgaagcagaa gtagcggtaa actgggtacc
     3001 tgaattagga acagttattt caaatgaaga agtgatcgat gggaaaagtg aacgtggtgg
     3061 ctatgatgta gtcagaaaac caatgcgcca atggatgctg aaaatcactg cttatgctga
     3121 tcgcttattg gatgatctag aattagttga ttggccagaa agcattaaag aaatgcaaag
     3181 aaattggatc ggtcgttcag taggagcaaa tattgaattt aaagtggctg gtacggataa
     3241 atcctatacc gtatttacga cacgaccaga tactctattc ggtgcaactt actcggttct
     3301 tgcacctgaa ctagatcttg ttagagaaat tacgacccct gaacaaaaag ctgctgtaga
     3361 agcttatatc gaagagacag ctaaaaaatc agatctaaag agaacggatc ttgcaaaaga
     3421 aaaaacagga gtatttactg gtgcttatgc aatcaatcca gttaatggca aggaaatccc
     3481 aatctggatc gccgattatg tattatcttc atacggcaca ggagccatca tggctgttcc
     3541 agcacatgat gaacgtgact ttgaattcgc tcaaactttc ggactagaaa ttcttccagt
     3601 tatcgaaggt ggagatgttg aaaaagctgc ttacacagaa gatggtacgc acatcaactc
     3661 tgaatttcta gacggcatga acaaacaaga agcaatcgat aaaatgaatg aatggctaga
     3721 agaaaatggc gtagggaaaa aagaagtcag ctatcgctta cgtgactggc tattctcccg
     3781 tcaacgttac tggggtgaac caatcccaat catccattgg gaggacggaa cagtcacagc
     3841 tgtcccagaa gaagagctac cattacggtt gcctaagaca aaaaacatca agccaagcgg
     3901 tactggagaa tctccattag caaatatcga ggagtgggtc aatgtcgttg atcctgtaac
     3961 aggtaaaaaa ggccgtcgcg aaacgaatac aatgccacaa tgggcaggaa gctcatggta
     4021 ttacctgcgt tatatcgacc ctcataacaa aaaagaacta gcagattatg aaaagttgaa
     4081 gcgttggcta ccagttgaca tctacattgg tggagcagaa catgcggtac ttcatttact
     4141 atatgcacga ttctggcata aattcttata cgatatcggt gttgttccta caaaagaacc
     4201 attccaaaaa ctatacaatc aaggaatgat cttaggcgaa aacaacgaaa aaatgtcaaa
     4261 atctcgtggt aatgtagtca atcctgatga tgtggtcgaa acttacggtg cagatacact
     4321 gcgcatgtac gaaatgttca tggggccatt agatgcttcg attgcttgga gcgaaaatgg
     4381 cttagaagga agccgtaaat tcttggatcg tgtatggcgt ttgatcgttg atgagaacaa
     4441 taaaatgcgt gaccgtatca ctacattgaa tgatggcaag cttgataaag tgtatcatca
     4501 aacagtgaaa aaagtgacag aagactatga aaatcttcat ttcaatacag caatctccca
     4561 attgatggtc tttatcaatg aagcctataa agtggacgct ctgccttatg aatatatcga
     4621 aggatttgtt caactactcg caccgattgc tcctcacatg ggtgaagaac tatgggcaat
     4681 ccttggaaat gatggtggta tttcttacgc accatggcca acatacgatg aagcagcact
     4741 tgttgaagac gaagtagaag ttgttttcca agtaaacggt aaagttcgtg cgaaaagcaa
     4801 tgttccccga gacttaggaa aagatgagct agaaaaagca gcattagcca atgaaatagt
     4861 gcaagaatat attgaaggca aaacagtcag aaaagttatt gcggtaccaa acaagttagt
     4921 caatatcgtt gctaattaat tggctctttg tcaataagga ctgatgagtt gtgcaaaatc
     4981 aaatctgggc cagaatgaac ttcattctgt ctcagatttt ttgttatttc aaattgatta
     5041 attgattgat caaaaaggtt ttgattttca tgttctcaaa cacgttcaat gtctttttct
     5101 tctgaatggt cttgtaagag atctctgtaa tcatcaaatt tttgtctatt aatcttagta
     5161 ttttttgatg tataaactac ctgaaattga gagtacatac caaattcata aattttttat
     5221 tagtgggact agtgattgta tggattttga cgcataatga atgaagtatg agaaaaggag
     5281 agacaaatgg atcaagaact atttattatc gaagtattat cagctaaaca aaaaattatc
     5341 gagaaagcga aaaaaagagc gaaaagaata atgttcaagc ttttaagctt agtatttttc
     5401 tctggtttgc tttttttcgg agctgtatcg ttcctatatc gccatatgag tgcagtagtc
     5461 cgttcagctg aagggatgca aaaattaaat aatttgtatc acttttcgtg gggagttggc
     5521 ggaataacaa ttgttgtgct tattgttttt cttctactta tttggctccg ttaccatcaa
     5581 ttgtcgcgtc atttcgatca atggcttagt caaagagcac aacagttttt acataataaa
     5641 ccaacttctc aagatgatga ctatctttat tttgagaaga cttcgacgag tcaagctttt
     5701 cggatacaaa aaagtgattg ccagttattg ctgagatcac ttgatgattt acctatctac
     5761 ttaggcgaaa gcaaatcaaa attcggaatg aacacattcc atattttttt actagaaaac
     5821 gaacccagat gggaaaaaag ccagtcttcg cctggtggat acctgacaac taagatgagt
     5881 acattagtca ctggtttttt cctcattctt ttagctggaa caatctatta tgggagatac
     5941 actcagccaa aaatgtcttc tcatataaat gctgcttctc acatgacaat gaactcaact
     6001 aacgatacgc atatacaaaa gacaaatgaa gggttgttag ctgatcctaa gcaaaaggta
     6061 aatttgacaa cacaaaagat aaatgatctc aaacttgata cagagacgca ctctctctat
     6121 atgacaacag atagtggtgc taattgggta tttgtgccga ttgattcgca atggttgcgt
     6181 tttggcgaat acaccctgac aacggggctg attcctgaag gttattggat ggacaacaca
     6241 tatgatattt ccgccgattt ctcatggttt atctattctt ctggggatga tgtacgtctg
     6301 ctgacttcaa gagataatgg taaaacatgg caaacgtcta ttatcaccca atcagttcag
     6361 ccgatacgtt atcgaaaagc ggtattttat ggcaacgatg gtctatttat ctgtacatca
     6421 gccagtggga tgtctgcaga aactttgtac atttacacta ctatggatgg cggaatcact
     6481 tggataaaaa gtggaagtac aatgatcaat cagccagtac aaaatgtcag cttcgttaca
     6541 acttctttag gttttgtttc gacacgtgaa agtttgtttt atacaaacaa cggaggacgc
     6601 tcattcaaag aatcagttct gtcgattccg gaaaactatc agacaggggg gatagatctt
     6661 tttcaatcac caaatgaagt tactcaaata agcgcgaata cgttagagac aaaattttat
     6721 ttgttgaaaa aaggaccaat cgatgcaggg aaaatgtttg cctgcctctt ccgttcaact
     6781 gataacgggg aaacatggca attcgttcag caactatcgc aagtagaaca aggagattaa
     6841 agaagagaga ttgtgagcct gacgttcagc accgagtcac aagaaacaaa ttcaaaaaac
     6901 agcttctcat atttgttgaa tagtgtaact cgtgattcgg agttgacaag agcaacaaga
     6961 agcgattttg aagtatagct tctcgtattt tacaagatcg tgtcttattg ccgataggtt
     7021 gtctcttgaa caccgtttat tcgtaaaaag ggattgtgac aagtcttttg tcactactta
     7081 ccgaacgaaa ctgaaggagc tatccaaaaa gtatcattag tattattctt tgtgtttcta
     7141 gtggcaccac tagtacattt aaaaaagcta tcgatcatgg actaatcata catgatcgat
     7201 agctttttag ataatgtttg ttacgctatc tgaaattgac tattataaag actggcataa
     7261 agcgttggtg attggagtag ttcagaatgt gttccttttt cgacgatatc gccattcttc
     7321 attacaagga tcatgtctgc attttcaatc gttgacaaac ggtgtgcaat cacaaaactt
     7381 gtcctatctt ttgtcacagc ttccattgct tgctgaatca atgcttctgt tcgtgtatct
     7441 acgctagaag ttgcttcatc taaaataacg acaggaggat tcgctagaat gactcgagca
     7501 attgtcaata attgttgctg tccttgagat aacgtgccgt tttcgctaga aatgatcgta
     7561 tcatatccgt ctggcagtgt tcgaataaag tgatcacatt gtgcgatttc agccgcttcg
     7621 ataatttgtt ctcttgtggc atccattttc ccgtaggcga tattatcagc taccgttcct
     7681 tcaaacagcc aggtattttg taaaaccatc ccaaaagaac gacgtagttc tccacgtgaa
     7741 agcttggtaa tatcaagacc gtcgaattcg atctttcctt tattcagttc ataaaaacgc
     7801 atcagcagat tgactaaagt cgtttttcct gcaccagttg gaccaacgat cgctaccgtt
     7861 tgttttggct ttactgaaaa atcgacttga ttcatcaata ttttatctga tgtgtaacca
     7921 aactgtacat ttttgaaagc tatttttcct ttcggttgtt ctaaaagtct tgcatctgga
     7981 aggtctggaa tttcatctgt ttcatctaat atctcaaata tcctatcgat tgctgccatt
     8041 gcggattgga tcgagttgat cacataagaa gcggtagaaa ttggttcaga aatctgattg
     8101 atgtactgaa gataagcttg taagaagccg atcgtcacac cacctgatag aacaagcata
     8161 gcaccgaata ccgcacttat gatgaaggcg agttgattaa tgaaacgaat ggccggatag
     8221 atagcacagt tcataaattg agcacgttta aaagcgttgt attgcttttt atttacttcg
     8281 tgtatagcag cttctgcatg agcttgctga ttgaatgttt tgaccactag attaccagct
     8341 aagatttctt ccattcgatt atttaaaaca cccagttcat actgattctg ttcagaaatc
     8401 tgtttgtttt tgttcgcaat cttagttgta acaattgaac tgccaacgat cagcaataag
     8461 acgagcaaag taagcttgct ttcgatataa aacagcatca gaccagcagc agctattgtt
     8521 acgacagaag taaaaaattg attgatacct gtcaataaca cttgagaaag ttgattgaga
     8581 ccggtagttg tccgactaat gatgtcacct acttgatgat tgtcaaaaaa cgacatcggt
     8641 aatgttttga atttttttgt gatttctttt cgcaccctca atgtgatctt ttcgcttaac
     8701 gctgccattg tccgttcctg aataaatgaa gtgatgctgc ttaagactga acagccgatc
     8761 agcaataaga cagggaacaa caatgcttgt ttgatatcac tgaaagaaag ctgtccaata
     8821 cctgtttttc ttatttctga taagagatca tcgatcccga ttcccatgat caagggcatg
     8881 gctacgacta ataggttccc gaataaactg catagaaaca atccgtaaaa atatttatgt
     8941 tcaggtttga tcattttcca aaaacgtatc aaagcacgcg tgttcaacgt ttttttcttt
     9001 tccatcgtat cccttctttc tatattcctt gtgattttgc aaactcctga taagtttggc
     9061 aattagacag tagttcttca tgagtacctt gtccaacgat ttttccttca tctaaaacaa
     9121 tgatgttatc tgcttgttgg attgtactga ggcgttgagc aacaatcacg agagttttct
     9181 ccgacatgtg tttatgaagg gctgctctta atgctgcatc tgttttatag tctaaagcgg
     9241 agaaactatc atcgaataca tagatctctg ctggtttgat caacgctcga gcaatacata
     9301 tccgctgttt ctggccccct gaataattag aaccgccttg agcaacaaag ctgtccaatc
     9361 cgtcaggaag ggtgtccact agttcttcta attgagcgat cttgatcgct ttagacaact
     9421 cttctgttga ggcatattca tttcccatct gcaagttgct taaaattgtc ccgctgaata
     9481 agaaagcctt ctgagggaca taactgatgt gggagcggat ctgttcttga gtcatttgag
     9541 taagaggaat accaccaagg gttacttttc ctatcgtagg gtcactaagt ctcaagagaa
     9601 gtttcgccaa actacttttt ccagatccgg tcccgccaac gatcgctgtt gtttttcctt
     9661 taggtattgt gaaatcaata tcttctagaa ctggatcaga gatttcagta tatttaaatg
     9721 tgacatgttc aaaagcagct gcatttgtct gatcaaaggt tggttcagct actgttttga
     9781 tcgtctcttc ctcaatttcg atctctgctt gcagtatttc ttctatccgt cgtaatgagg
     9841 caaacgattt agggataatg accaatacca tagctgcgat catcaagtaa gcaagtgtca
     9901 gcatagagta ttcgactaca gcagtcacga caccgatttg taatgctcct tctgctacaa
     9961 gatttccgcc aaaccacaaa atagaagcat aaacgattcc catgagcaaa aaagcaattg
    10021 gagtgaccca agcaaatatt cggttcactt tgatcatttg attagcatag tcagcaaagc
    10081 tcttattcgt tcgcttttct tcatatgatt gattatcaaa ggcacggatc atattaatcc
    10141 cagtaaagaa ttctctcaat gtgatggtga tccgatccat ttttggctgg atactcaaag
    10201 ataaaggtgc ccctcgtttc atcagtcgat aaacaatcca tacataaaaa acaacagaga
    10261 tcaatgtaac ccatgttagt aatagagaat aacgaaacat catgagaatg gtgaaaacgc
    10321 aaataaccgg tgctggtaag atcatttggc aaaataaaac aatcatttgc tggacattat
    10381 cgacatcatt cgtcattctt gtcaaaagag atccagaact aaaacgactg atatccttca
    10441 ttgacagctg ttggaattta cggaaatatt tctcccgcgt ttggaatcca aacctagcgg
    10501 aagccaatgc tgaaaagtaa ctagaggcaa ctgcagcgat tgttcctaga aacgctacaa
    10561 gcagcatttc aactccaatt ttttttataa ctgaggcttc gccacttgct atacctgtgt
    10621 cgatcagctt tgctactaat tttggtacac ctaatgtacc ggcgatttct aaacagatca
    10681 tcacaaaggt aaggagagcg agtcctttgt ttttctgtaa gaatgttttt aagatagaca
    10741 tattattcct ccttttttcc tgatactttt cgattctata ccttcaagta acttgaaggt
    10801 caatagaaaa aagaataaac tgaaaaagag gtgagaaaaa atgaaaaaaa tgtatacgat
    10861 tggccaagta tcggaactat ttgatttgcc aaaatcaacg atccgctatt gggatgaaca
    10921 aggattaatc cgttcaagta gacaagaaga gaatgattat cgattgtttg atatcgatga
    10981 tatttttatg ctttatgata tcgtttttta tagaaaatta gatataccga tcaaacagat
    11041 gaagaattta tatggaaaaa cattgacgga gttatatgag acacttgatg aaacagaaag
    11101 aagaatccat aaagaactgg tggtgatgaa acaaaaacaa aaagagataa gggagcaaaa
    11161 aaaacagttg aaattgatga tcgatacaaa cgaggaagaa tttcctgtag aagagatccc
    11221 ttttgactgt atgatctcaa cggagtttga agatattgtg gaaatcaaaa aatttctacc
    11281 taactattca agctttggga tgatgagtat gccagcttcc agttcacaga cgatgtatgg
    11341 attttttatc gaccctagtg aagtacattt atttactcaa gacgtgattt gggagaaaaa
    11401 agatacggct gtttatcgtc gattcttatt gaaatccgag atgaaccatg cggaacgtaa
    11461 taatatttta gaaatcagag aaagaatgta tgaaaaagga tggaaaacag gagaagtgat
    11521 cggccagtat ctattgacga atacggatga aaacaatatc agaactgaat attatcatgc
    11581 atggatagaa atgaaaaaat aggaaatatg tgtttaagat aactcgaaaa tgatggttgt
    11641 tttagttata aagaaaaatt ttctggttcg attacgggtt taatgtgggg gattattagt
    11701 cgtggataga tagaatttta gttttttaga ggaaatatgt ctttaaaaat ggaatggttg
    11761 caattcttag cgatatatac gatgtaattg tcctcttaaa aaagtatgga aaaataattg
    11821 tattgggtgt tatgtataat tcatggtgaa ctgagactaa tgagaagaaa aattcatatt
    11881 gttatgagtg agattgtatc agat
//
LOCUS       DAYYME010000088        11837 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000088 DAYYME010000000
VERSION     DAYYME010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.089"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..177
                     /locus_tag="ACHBFW_001957"
     CDS             <1..177
                     /locus_tag="ACHBFW_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9318258.1"
                     /translation="YLLGMALAYSVGSLIKVNVSLDLFTILLAVGVSSVIGLVFSVMP
                     ASEAAKKDLIDILR"
     gene            578..1183
                     /gene="fic"
                     /locus_tag="ACHBFW_001958"
     CDS             578..1183
                     /gene="fic"
                     /locus_tag="ACHBFW_001958"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFM9318259.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1199..1771
                     /locus_tag="ACHBFW_001959"
     CDS             1199..1771
                     /locus_tag="ACHBFW_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9318260.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2023..2280)
                     /locus_tag="ACHBFW_001960"
     CDS             complement(2023..2280)
                     /locus_tag="ACHBFW_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318261.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2406..3221)
                     /locus_tag="ACHBFW_001961"
     CDS             complement(2406..3221)
                     /locus_tag="ACHBFW_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9318262.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            3738..5063
                     /locus_tag="ACHBFW_001962"
     CDS             3738..5063
                     /locus_tag="ACHBFW_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM9318263.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5056..5406
                     /locus_tag="ACHBFW_001963"
     CDS             5056..5406
                     /locus_tag="ACHBFW_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318264.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5403..5573
                     /locus_tag="ACHBFW_001964"
     CDS             5403..5573
                     /locus_tag="ACHBFW_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318265.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(6477..6752)
                     /gene="prgO"
                     /locus_tag="ACHBFW_001965"
     CDS             complement(6477..6752)
                     /gene="prgO"
                     /locus_tag="ACHBFW_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFM9318266.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(6724..7677)
                     /locus_tag="ACHBFW_001966"
     CDS             complement(6724..7677)
                     /locus_tag="ACHBFW_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9318267.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8289..9782
                     /locus_tag="ACHBFW_001967"
     CDS             8289..9782
                     /locus_tag="ACHBFW_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFM9318268.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            9917..10213
                     /locus_tag="ACHBFW_001968"
     CDS             9917..10213
                     /locus_tag="ACHBFW_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM9318269.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10314..11165
                     /locus_tag="ACHBFW_001969"
     CDS             10314..11165
                     /locus_tag="ACHBFW_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318270.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11186..11398
                     /locus_tag="ACHBFW_001970"
     CDS             11186..11398
                     /locus_tag="ACHBFW_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318271.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4075 a         1820 c         2197 g         3745 t
ORIGIN      
        1 tatttattag gaatggctct tgcttatagt gttggttctt tgataaaggt aaacgtgtca
       61 cttgatctat ttactatttt actagcggtt ggggtatctt cggttattgg actggtcttt
      121 tctgtaatgc ccgcttcaga ggctgcaaaa aaagatttaa ttgatattct tagataaaat
      181 cagaatgaca agaatattta cgttctagat tctaaaataa aacacaaaga gaccaaccag
      241 gcaccctatt gtttctttgt agtatagaga tattctttgg cttgtaagag atcaatagag
      301 ttaggtgacc ggagttcgaa ataaaggaaa tttttgattg tataggagaa ttctttatcc
      361 ctaatattta gaattttgag tatgccatgt tgatgtttta gattctctat aaatatagag
      421 gtgtatcaaa ggtcaactag actgtgcaaa atagagacac ttgaaaaatc tatcgggggt
      481 cagaaactta atagtttttg acacataaac aaaatcgcga tataatgggg ttgagagtgt
      541 ttcataaact tatgtcataa agttaaggag atttcgcatg attttagaga ataaattggg
      601 attaaccaat caagttgagt tggctaaggt tgaagaaaaa ctcagtaaac aaaaagcgaa
      661 agaattgtac gattgtggaa aaataaatga aatcgaagtt ggtacattta aaggtttatc
      721 tgaaattcat gaatttctat tttcggatat ttacgatttt gccggaaaaa tccgatcggt
      781 caatattgca aaaggaaatt ttcgatttgc accagttatg tatttagaac attcgttaca
      841 gcatattgat cagatgccac aaacttcttt tgaagagatt atcaagaaat atgtggagat
      901 gaatatcgct cacccatttc gagaaggaaa tggccgaagt actagaattt ggctggatct
      961 cattttaaag gaagaattgc aaaaagttgt agattggaac ttaatcgata aagcagacta
     1021 tctttctgca atggagcgaa gtcccataaa cgatttggaa atacgctatc taatttccaa
     1081 tgctctgact gataaaattg acgatcgcga gttatatatg aaaggaatcg atgtcagcta
     1141 tttctatgaa gggtattctg agtatacgat agatgatttg taaaagtagg tggaccaaat
     1201 gacgaaatat ggatatgcac gtgtctcaac cgcttctcaa agtacaaaag aacaaattca
     1261 acagttgctt gagaacggcg tagagaagaa gaatattttt tcagaaaaat ttacgggtac
     1321 aaaacgggat cggccaaaat tcaatcagct gttggctggt ctgaagcctg gagatgaaat
     1381 agttgttacg aagcttgatc gatttgctcg taacacgaga gaagcattag agatcattga
     1441 acctttactc gatgataacg taacgatcaa ggttttgaat ctaggaacga ttgaaaatac
     1501 gtccatgggg cgaatggtga cacggacgtt gttgagtgta gcagaaatgg aacgtgatat
     1561 gattgtggaa cgtacccaag aaggaaaaat gtttgcaaga aagaataatc ctaatttcag
     1621 agaaggacgt ccgaaagcaa caatcacacc gaagaagagg cacgcatatg aactgcttac
     1681 ttcaggtaaa tcgtataagg aagttgaagc tataaccggt ttcagtcgca gtacgctttt
     1741 tagaataaag aagaaaattg aagaaagtta aaaaagaagt ctcagcgaac cagacaggta
     1801 aatacttaat gcgttctaga tatgagtcta ttagcatgtt gtgaaatata atattttttt
     1861 atttacttga tttctgctca aaaaatgtgt atggtaactg taaaaggtaa agtaattaaa
     1921 ggtatgctag ccacgcttac tatattagga taccatcata tatttataat gaacgatttg
     1981 ttcgtctacc aaacaacaca tatcgttcag cgtaagtcac gtttaggaac gtggcttttt
     2041 tagtgttatt atcgataaat aatctattga ctctattgat tttatctcca tatccctacg
     2101 gtgttgattg tctttagatc tttcatttaa gaaccaaata actatcataa aaatcagcac
     2161 tgctgctaat ataataaaca gcattcctat tgtgatatta cctattgatt ttaccataac
     2221 tacgattaaa gtaataatgg cattcaagtc tcctttgttc tctatatata aattagccat
     2281 aaatcatctt cctttcaaat taagatactt gtttgatacc aataaatagt aagcgtggct
     2341 agcatacctt tttctgtaat agcaagtgtt ctaatagtcc aaacttaatg atggaccgat
     2401 gctagttata gtacagtaat gttttctttc ttatctagat ttttagtaag ctctatcagc
     2461 aaatctaaca aatctgagtc cttttcagaa actaaattaa atctagtgtt tacaacaaag
     2521 ttttcatgtt ctatattgtt tagattattt gcggaaagga gggcttctag atcttgacat
     2581 tcaaatctat atgcatcgtc ataaatctta ctagaaggag tgcttaattc gaaattactt
     2641 attttctcat cttggaccaa gataaattta tagatagtat ctttttgtgt ttttctttta
     2701 aaaacacaac agagtgtgtt ataaggaacg ttataatttt catcagcctc attttttctc
     2761 cattcaaaca aatgataaac tgcaaccaag ttgccatcaa tttcatggac agtgctctcg
     2821 gtaacatcct ttaaagaact atcaagggct aatttatagt aactagagag aggaagtaaa
     2881 attgtcgatt tactgagatg gttatcgtct atacgtgcaa tcatacctaa atcaactaag
     2941 gttttattcc aggtgtttat agttttagtt gtcactccta gagactctgc tatggtattg
     3001 accgaatgcc aggaatcccc gccttcattc tttgccttaa aacagtaata cagatataaa
     3061 ttaatagctt tgttattcgg taaatatttt tcaagttctg ttggtagaat aaagtaagaa
     3121 tccttaatgt tttttctgaa ctgtctccag tctctgtagt tttgtttata aagttgtgaa
     3181 acggtttttc cacctttaga aagtcctgct atcttaacca atcttattgc tcctttcttg
     3241 aatctgtcat gatcatacag tattttttac ttgtagtcca gcaccttaat atattatcct
     3301 atgtaattaa tttcgtacta gatagtaatt attttttatt taattctcta gtcacttaca
     3361 aataagttat ataaactacg attaataaat tattacaaaa actattaaga aaacttctac
     3421 atacaactat tacaaaacta ttaagaaact gaatactcct attttttgtt tttaaggaaa
     3481 gttttttctt ttttgaattt cttatttttt tctaatataa ctaagcaaaa ctggataata
     3541 cgacacttat tttcaaattt tcacaagact aatagtaaat atccatttaa taaagtgata
     3601 caatgtgttg attttacaaa tcaacagaag aagtagggaa ataacttacc tatgcttctt
     3661 tttttgtaca tttaatcgat tcttctttac aaaaacgaac gtatgtttgt ataatgtgga
     3721 agaatggagt gttatttatg atggattatt caaaagagcc agtaaatgat tactttttga
     3781 tcgatatgaa aagcttttac gcaagtgtcg aatgtataga aaggaattta gatccattaa
     3841 cgactgaatt agtcgtcatg agtcgagcgg ataatacagg atcagggttg attttagctt
     3901 cttcaccagc tgcaaagaag aagtacggta tttctaatgt cagtcgacca cgagatctac
     3961 cgcaaccttt tcctaaaacg ttacatgtcg ttccaccacg catgaatctc tatattaagc
     4021 gaaatatgca ggtaaacaat attttcagaa ggtatgtagc agacgaagat ttattgattt
     4081 actcgatcga tgaatcgatc ttaaaagtga ccagatcaat gaatcttttt acgactgaag
     4141 gaacaagaag tcaacggaga aagaagctgg ctcaaatgat ccaggagaat attaaagaag
     4201 agctaggatt gcttgctaca gtaggtgtcg gagataatcc cttattagct aaacttgctt
     4261 tagacaatga agcaaaacat aatgatggat tccttgctga atggacgtat caaaatgttc
     4321 ctgataaagt atggaatatt cctgaaatga cagacctttg gggtattgga tctcgaatga
     4381 aaaaaagact caaccaaatt ggtatcatga gtattaaaga cttagctaat tggaatccat
     4441 acactattaa gagccattta ggtgtgattg gcttacagct gtattttcat gcgaacggga
     4501 tcgatcgaac agatatagcg atcccaccag aaccgacaaa ggaaaaatca tatggtaatt
     4561 cacaagtgtt accacgagat tataccagga gaaatgaaat cgaactggtg gtaaaagaaa
     4621 tggcggaaca agtagctatt cgtatcaggc aacataactg taaaacaggt tgtgtccatt
     4681 tatacattgg cacgtcgatc ttagaaacga gaccaggttt ttcacaccag atgaagatcc
     4741 ctataacaga taatacaaag gaattgcaga attactgtct tttcttgttt gataagtatt
     4801 atgaaggaca agaagtaagg cacgttggaa taacctatag caagcttgtg tacactgatt
     4861 cgcttcagct ggatttattt agtgatccac aaaagcaaat tgacgaagaa aatttagata
     4921 aaattattga caagattcgg caaaaatatg gttttacgtc gattgtacat gcttcttcta
     4981 tgttagaagg cgctcgtagt attactagaa gtacattggt tggtggccat gcaggcggcg
     5041 caggagggct taaaaatgat tgattataat gatcgtggac gtttaaagtg ggcaggtttt
     5101 tatttatctg atcatactga aaaaattgat gccgacaatg aacaacgagc caatcaaaat
     5161 ttggcaaaag aacaaatgac gactgaagag gttgcggaag tattgaacgc tgcagtcata
     5221 aaaaatagat cagtatgtat tcaaaaagaa gaacgaaacg ctgaaggata ctatccagaa
     5281 gatattgtta gcaagatctt aggtagtgat gagctgggga tctacgttgg aaaaaacaaa
     5341 gttcactacg acgaaatacg tcatatcgaa ttttaccagg ataataaatg gggtcgatta
     5401 gaatgaacat tatccagcaa tatgagctaa agtatataac ctttgatcag ctatcagaag
     5461 agatttgggg ttatggccag cgtttgataa atgaggttgg tgttgaacgc ttctctttct
     5521 atgtagaagc tgcagcagga taccataaat acgaatacta tattggctat taaagtgcaa
     5581 taaaattata tttcttttat gttttacatt ttgtatcaat tctgataaaa ttacctatag
     5641 gtaattaaaa aatataaaaa aactagttac aaccttagga cgttgtaact agcttgaagc
     5701 atactagagc taactaaaat agcactagaa agcaatgtta aacccgtcca acaaacttga
     5761 tttaacattg agaccagctt attcagctaa atatagttga ataagcttct tttgttttaa
     5821 aaaaacaaaa ggtgtactac gaataacgca agtaaaagaa atactattac aaccagtagg
     5881 ctacaagtaa gtattactaa acattgcgta gttagaacct ttgctctatt gtctaaagcc
     5941 acaaaattag agatcagctt tatgatgcta agagcgagct gcatccatgc ggacataggt
     6001 tcttttgaca ctattaattc tcctttttag gataagggaa attgctagta gttttttcat
     6061 tagcaccatc ccccttcatt tgattgagtc tagtgctgct gtgatagttt gctgtttaat
     6121 atgcttacat tcttattata acataaatga aacaaacaca tggttagagg tcgtatcaat
     6181 caaaatagtg tctcacaaac gatttattac ttactcctgc tgactaacgg actgttcttc
     6241 agtaactgta actatatgtt tgttaaaacc accattacct gtagataaaa accaatatat
     6301 ttactatact cactatatta aatatattta gtatagtaaa aactagaaac aataaaattt
     6361 gtgaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
     6421 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
     6481 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
     6541 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
     6601 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
     6661 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     6721 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     6781 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     6841 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     6901 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     6961 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     7021 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     7081 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     7141 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     7201 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     7261 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     7321 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     7381 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     7441 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     7501 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     7561 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     7621 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     7681 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     7741 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     7801 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     7861 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     7921 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     7981 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     8041 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     8101 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     8161 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     8221 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     8281 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     8341 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     8401 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     8461 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     8521 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     8581 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     8641 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     8701 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     8761 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     8821 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     8881 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     8941 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     9001 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     9061 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     9121 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     9181 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     9241 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     9301 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     9361 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     9421 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     9481 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     9541 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     9601 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     9661 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     9721 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     9781 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     9841 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     9901 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     9961 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
    10021 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
    10081 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
    10141 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa aaaggaagaa
    10201 gtatatccag tagtagtttt tatttactta aaaaattggt tcattgagca acctatcgct
    10261 gctaaagtag caaaggaaaa cgttaaagga aagaatttag ggaagaaatc gaaatgaaat
    10321 cggacgactg gattgaacac acctggcttc ctctgaaaag aaatagcaaa cgaaagaaat
    10381 tttgggtatt gcaattatgg ccattaggaa aactttgtag ttacggggca cgacttattc
    10441 ttgatttaac cgtttactcg attggagtat ttggaacggc ttattacttg ataaatgatc
    10501 ccaataggat tgcagcatca gacattttta cagtgctaat gacgttgatt tttgtcatgc
    10561 tattgtctat ttttactcaa ttagtcatgc aatttaccag gcaaaaatta acagcaggaa
    10621 aacttttcaa aagcctattc gatttaattg ttgtaaatgc aattctagcg actttaataa
    10681 aacaaattga acaatctgga agaacaacaa aaggagtgaa ggatttttta ttgacgttta
    10741 ttcactttgt aaatggacac atgtatttgt ttgaagtggg tattattctc ttttttgtcg
    10801 ggtatgtatc cacgaaactt attaaacgtt caattgaaaa agaaattgaa attgacctga
    10861 ttccgacact tcttggttat gagtacaatg ttgattttgg aagatatgac gaattgatca
    10921 atacggatat cgttcacacg agtatccatg aatctactgt taagggcatc agatctattt
    10981 taaaaaatgg ccaggaaatg aaagcaatag atcgaagaaa agagatctac aatttttatg
    11041 aagtcgaagc aagcctacga tatggcatcg gaaaattgtg gttagatgga agtaatattc
    11101 gtcaaaacaa accatacatt ttttggaaca gtagtgattt agaacttcca atgaaagata
    11161 aataattatg gagaatcgtt tgaaaatgat tcctctatac aggattgtgc ttatagttgg
    11221 catggagtta aaaaccttaa agaaggagca gttattcaaa gtgaaagaac gtcataatca
    11281 gttactatgt agtaaaacgg gtacgatgaa cattgaatac cgaacagaat atttagaatt
    11341 aacgaatcaa actgattttt tgttgattcg aaagcaacaa gagcaagaaa aaaattaatg
    11401 gctacttata gccaattgat tgatgaatgg gaacaattca aggaatctaa aattccagaa
    11461 cgtctctaaa agcctttatt tgtaaagagc ataaggaaac gtgggaacac tttttaaaaa
    11521 aagtgttcca ataggctgtg cctattacgt ttctcaaaaa taaatgtggg aacaaaatat
    11581 ttaaaaatag gatagtgaaa ggatagtgaa ggatagtaaa aggagggtag taggatagca
    11641 aaaaggatag gctccttttc gctatccttt ttaaattgga ggaattaata tggaagtaag
    11701 aataagaaat gtggatgaga aaatcgtaag agaaattgat cgaagatgtg acgaacggtt
    11761 ctgttgcaaa gttttaaatc tactatcaaa taaggtagaa taatagaaaa agatagcagg
    11821 aggaatgacg atgaatc
//
LOCUS       DAYYME010000089        11671 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000089 DAYYME010000000
VERSION     DAYYME010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.090"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..716)
                     /locus_tag="ACHBFW_001971"
     CDS             complement(111..716)
                     /locus_tag="ACHBFW_001971"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9318272.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            912..1835
                     /gene="coaA"
                     /locus_tag="ACHBFW_001972"
     CDS             912..1835
                     /gene="coaA"
                     /locus_tag="ACHBFW_001972"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFM9318273.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            1894..3459
                     /gene="guaA"
                     /locus_tag="ACHBFW_001973"
     CDS             1894..3459
                     /gene="guaA"
                     /locus_tag="ACHBFW_001973"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM9318274.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(3950..5581)
                     /locus_tag="ACHBFW_001974"
     CDS             complement(3950..5581)
                     /locus_tag="ACHBFW_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFM9318275.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(5595..7268)
                     /locus_tag="ACHBFW_001975"
     CDS             complement(5595..7268)
                     /locus_tag="ACHBFW_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFM9318276.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(7281..7598)
                     /locus_tag="ACHBFW_001976"
     CDS             complement(7281..7598)
                     /locus_tag="ACHBFW_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM9318277.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(7623..9023)
                     /gene="lacG"
                     /locus_tag="ACHBFW_001977"
     CDS             complement(7623..9023)
                     /gene="lacG"
                     /locus_tag="ACHBFW_001977"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM9318278.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9260..10045
                     /locus_tag="ACHBFW_001978"
     CDS             9260..10045
                     /locus_tag="ACHBFW_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9318279.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            10048..10740
                     /locus_tag="ACHBFW_001979"
     CDS             10048..10740
                     /locus_tag="ACHBFW_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9318280.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(11014..11385)
                     /locus_tag="ACHBFW_001980"
     CDS             complement(11014..11385)
                     /locus_tag="ACHBFW_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFM9318281.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         3558 a         2493 c         2119 g         3501 t
ORIGIN      
        1 aaaaaatagt tctttgtttt taaaaacttc tcaacaaaaa aagaactgat acgtacccgt
       61 acatgcacgc ccagttccac tctttcgtcg ctccactatt tttcagctag ttattctttc
      121 acacttttga tgatataata tcctttatct tttgtgacga tttctgcatt accaaatgtt
      181 tcttccattt tcttttgcgc actcggcgct ccttgtttct tctggatcac aattgtcagt
      241 gtaccgccct tcttcaaccg aggatatgcg cctgtcaaaa tttcatgtac aacttttttt
      301 ccagcacgga tcggcggatt gcttacgata gctgcatagg tttcttcatg aactgcttca
      361 tagatattcg attgatggat atcaacatgt tcgatttggt tacgctttgc gtttccttgc
      421 gctagactta ccgctcgttc attaatgtcg accatttcaa caaaccgctg tgtcgcaaat
      481 gccaaagcta acccaatcgg tccatacccg catcccacat ctaagatctt tccttcatca
      541 ggtaactctt cccaatcgaa agcatcaatc aggacacgtg aaccataatc cactgtttca
      601 cgtgaaaata caccagaatc tgttacaaat tgaaaggttc tgccttttaa ctcaaaagcc
      661 cattgttcaa aatcgtgcgc cgtatctggg cgttcagaat agtaatgatt tgtcatgtct
      721 tccatccttc tcatttcttt catgacaaca gtctatcctt ttatgtatcc gtgcgcaagt
      781 caagaataag actccgtttc tttctattat aaagattata caaaaagatc ggcaatctct
      841 cttaagtggt ggggtttgcg tttattttgt gttaaaatag ttccattgat gggagaacgc
      901 aggaggctaa tatgaatgat ccaatgaact actaccgtgt ggcaagagaa gaatggcgag
      961 aattttatcg taatgggcaa gcacctttga cacaagatga actagataat atcaaaagtt
     1021 tgaatgaccg catttccatg caggatgttc gagatatcta tgtacctctt gctcatctga
     1081 tttatcttta tatgaaagaa tttgaatctt tgttcttgag taaaggttta tttcttcata
     1141 aatacgtaga agtaccgcct tttattatcg ggatcgccgg aagtgtagcc gttggaaaaa
     1201 gtacaacagc cagactgctg cagacgattt tgtctcgtac cttcaaacga agaaacgttc
     1261 agctgatcac gactgacgga tttctttatc caaatcgtat attaaaagag cgtggaatca
     1321 tggaccgtaa aggatttcct gaaagttatg atatggagat gctgatcgat ttcttgaacc
     1381 gtgtaaaatc tggtcaggaa gagataaaag tacctgttta ctcacatgat gtgtatgata
     1441 tcattccaga caagtacgaa ttgatccagc agcctgatat cttgatcgtt gaggggatca
     1501 atacgcttca attacctgcg aatcagcaaa tctacgtcag cgactttttt gatttttctg
     1561 tctatgtcga tgccgatccc gtgctgatcg aaaaatggta tttggatcgt tttggtgcgt
     1621 tactggatac ggctttccaa aatccagaga attattatta tcgttttgct atcggagatc
     1681 gtgaagaagc ctttaaaatg gcaaaagatg tatggaaaaa tgtgaatttg aaaaatttac
     1741 aggaatatat cttgcctaca cgaggaagag ccgatataat tctccataaa actgaaaatc
     1801 atatcatcga ttcgattttc atgcgaaaat attagcattt taaaatagtt tgactatttt
     1861 gagataaaat ttaaagggat aggggtaata aatttgacaa acgttgccga tatgacgaac
     1921 gttgaaaaga ttatcgtatt agactacggt agtcagtata accaattgat cacaagacgt
     1981 attcgtgatc ttggagtctt ttccgaatta ttgagccacc gcattactgc agaagaagta
     2041 aaagcgatga atccaaaggg aatcattctt tctggcggac caaacagtgt atatgatgag
     2101 aatgcatttg ggatcgatcc tgagatcttc aatttaggta ttcctgtatt agggatttgc
     2161 tatggtatgc aattgatcac gtacaaactt ggcggttccg tagagcctgc taaaaaccgc
     2221 gaatatggga aagccttgtt agaagtaaca agcgatcaag ctgtcttgtt taaagaaaca
     2281 ccaaaacaac aaacagtatg gatgagccat ggtgacttgg taactaaagt tcctgaaggc
     2341 tttgaaacag tcggaacaag caaagattgt ccgattgctt ctatccaaga cacaacacgt
     2401 aatttttatg gtatccaatt ccatgcagaa gtacgtcatt ccgaatacgg aatcgaattg
     2461 ctgcgccact ttgcctttga tgtttgtcac tgtgaaggta actggacaat ggataacttt
     2521 atcgatatgc aagtagaaaa aatccgtgaa caagttggtg acaaaaaagt cttgcttggt
     2581 ctttcaggtg gcgttgattc tagtgtcgtt ggggttcttt tacaaaaagc gatcggtgac
     2641 caattgacat ctatctttgt tgaccacggt ctgctacgca aaggcgaagc ggaacaagta
     2701 atggaaagtc taggcggaaa attcggcttg aacatcatca aagtcaatgc aagagatcgc
     2761 ttcttagaca agttgaaagg tgtatctgac ccagaacaaa aacgtaaaat cattgggaat
     2821 gaattcgtat atgtttttga tgacgaagca acaaaacttg ctggtgaaga aggcgtctct
     2881 ttcctagcac aaggaacgtt gtatactgac gttatcgaat ctggtacaga aacagcacag
     2941 acgatcaaat ctcaccataa tgtaggtgga ctgccagaag atatgcaatt tgaattgatc
     3001 gaaccattaa acacattatt caaagacgaa gtccgtgaat tagggactca gttaggcatg
     3061 cctgattcaa tcgtttggcg tcagccattc cctggtcctg gtcttggtat tcgtgttctt
     3121 ggcgaaatca ctgaagacaa gctagaaatc gtacgcgaat ctgatgctat cttgcgtgaa
     3181 gaaatcgcag ctgctggact tgaccgtgat atctggcaat atttcactgt tcttccaggg
     3241 atccgctctg tcggtgttat gggtgacgga cgtacttacg actacacagt cggtatccgt
     3301 gctgtaactt caatcgacgg aatgaccgct gactttgccc gtatcccatg ggaagtcttg
     3361 caaaaaatct ctgtacgtat cgtaaacgaa gtagcccacg tcaaccgtat cgtgtatgac
     3421 atcacttcta aaccaccagc aacagtggag tgggaataaa gagccaacat aatatggagg
     3481 gggaatccct ataaatcaac attcttcaat agctaaaatg cttgattttc attaaaaata
     3541 agatggttcc ctaccaaaag agactataat ataagttaat ttgtttcatt cttatcataa
     3601 aaagaagttc tcgaaaaacg aactgttttc cgagaacttt tttctatcca tctaagtgct
     3661 caaatcatat tcacgacgga attctaaggt taccctttct taaacaaact ttatgttatc
     3721 tgcacaactt tttgcaattt tttcattgct tcttacgaaa tcgttgaact ctctttagca
     3781 taatacaaag tgtagatgaa gtctggtgct ttactcgttt tggtaaaaat gactggaaat
     3841 cgtttctggt tatttttggc aaaggcgagg tgcaggttcg attcctgcct tctacgctaa
     3901 cgatgtggct gaagggaacc tccgatcttg aatcgaaacg atccgcagtc tattgtttca
     3961 ccaatgaaac aaaatcaacg ttcgtccaag catttcctga agccttgcta taaaccccaa
     4021 tttcacaaac acctgttgta attggaatgt ctttgattgt aatttttgtc cattcacttg
     4081 ttttaacaac cgcagtattt agttccgctc caccataatt tttgatatat aactgattag
     4141 tatcaaactc tcccccagac attacatagg cactcaatgt gtaagttcct ttgcttatgc
     4201 cactaacatt ttgataagct gatgcctcgt atgcttgatc ctgccaaaaa gttagtttgt
     4261 agtccccaaa aaaagtgccc gcttccgttt tgatcgtacc cttagcggtt ccttgttgaa
     4321 accaggtttg ccaattggaa ggactggtcg ttggtccatc gctttcgaaa cttgcatttt
     4381 taactagatt tatttgcgcc cctgcttgtt taaacgcacg caagcttggc aaggcgtttc
     4441 cagcaaaatc aaacatcgct tggttgtccc attcgtttcc catagtggat tgaacactga
     4501 gataattcat acctgcttgt gttgcccaac tcacatttcc gttgtaccaa agaggttccc
     4561 aatagaagaa gcccgccccg cgattattcg gtactgcctg aatccgatcg gcaagatcca
     4621 gcaaatactc tgcttgtcct gtcggtgttg gttcatatcc ccccgcagct gcttcggctt
     4681 caccaaaagc atttggcata gtatcggcat tcgctgttgt atgcccataa gctgtttcaa
     4741 ctacgatcac ttctttacca taccgttggc tgatcgcatt catattggtt tgaagttcat
     4801 tcattgaacc gtgccaatac ggataatacg acactccaat taaatcaaat gacacacctc
     4861 gactcgtgat ttcatcaaac caccaagtaa atgctcctgt atcgccacca tcagctaagt
     4921 ggagcattac tggagttgtt ttaggttgag tgtctcgaac agcttggata ccagacttca
     4981 aaaaagcagc caatcgatca aattctcccc cgccttctcc ccaagacttg ccataaggcc
     5041 agagcattcc gctattcagt tcattgccga tctgaaccat atcaggataa acgtccagct
     5101 gcttcatttc cgacagaacg ttctttgtat agtcgtacaa tgccgtattc atttcttcaa
     5161 aggtcagacc ttgccatgct ttaggaagat tctgtttccc aggatcaacc caaaaatcac
     5221 tgtagtgaaa atccagcagc accttaagtc ccagattttt tgctcgtttt gcaaggtcaa
     5281 tcgtcgtttc cagatcattt gtccctgctc cataactttc tccattacta ttataaggat
     5341 cctgccacaa acgcagtcga acatagttga caccattatt ttttaaaatc tctaaggcat
     5401 cttttgaaat atcgtcttca taatattttg caccagattt ttccatatct gacaggatcg
     5461 aaatatctgc tccgcgaata aattcgctgg cagatgcttc aacagttttt gaaacagccc
     5521 ccaaagcccc taacacagct gcaaataaaa cagctttacg caaccaaatt cccattttca
     5581 ttatggctcc tcctttattg aaattggttc agaataaact gaacagcttt ttcaggatct
     5641 ctcgttaatc cgatatattc tgctcctttt gtcgcaacca gcttgattcc taagcgatcg
     5701 gtgtcttctt tgatatcatc atagtaagaa ttgacttgcg gtgctaagac caccatatca
     5761 aaatcctgca gcatttcgta atgagaacca taagcacccg ccattgcact gatcggtgct
     5821 ttcgattcag atgcaccttt ttttaaagca ttagctaaca tcccacttgt ccctgcgcca
     5881 gcacaaagta ccaacacatt gatttcttta tttgcatcgc tgataacttg actcgctgcc
     5941 acaggtactt cctcacgtac atcggcagaa gcaccttcct cggttgcgat cacctctgct
     6001 ttttgtgctt ctttctctac taagacagca tcgtatgctt tgcaaaatgg tagatagatc
     6061 aggaagtcaa cgacgattag aaccgctgct agaacaaaag ctaagatacc catgcccgtt
     6121 cctagaacca aaccgatcgg tgctggagtt gcccaaggca aaacgtacat aaagctgttc
     6181 atgcccaata catcgacaaa aaacttgaac aggcaaacgt ttacaacagg tgttaacaaa
     6241 aatggaataa aaaaataagg gttcaaaatc atgggtgccg caaatagcaa tggttcgttg
     6301 acagcgaaac ttactggaat aaaggaagct ttcccaactg ccttcaactg cttagactta
     6361 gcaaagagta ggaagataaa aggaacaacc aatgttgccc cagttcctcc catagttcct
     6421 acaaaattcc ctaaacccac cgtcagtact ttagaagctt gttcaccgga attgaacaat
     6481 tgcagattgg tttccacatt ggcatagata attgccgcaa ttgccggctc cacgatcgac
     6541 ggtccatgaa caccgacaaa ccaaaacagc gccatcgctc cccagataat agcgattcct
     6601 aagtacccgt cagctgctgt aaatagcggc tgcagcaccg taatgattgc ttctgcaaat
     6661 gagatttgga agatttgtcg aatgcctaaa tcaattaaaa cggccgcaaa tactgaaaaa
     6721 gagaaaggaa aaatatctcg gaaggtttgc gaaatcgttc caggaacttc tttaggcatt
     6781 ttaatggtaa tatctttcag cacacaaaac ttatataaat taactgttaa aaatgcagcg
     6841 acaaaggaag ccagtagtcc ctttgtcccc atatattccg ttgaaaatcc accttcaatc
     6901 ggagagacgc ttaacaacag gaaacttacc acagaggcca gcatcacaga ggtggtgttg
     6961 attactttgt ttttcggtaa gcgacgattc atcgaatcag ccaagcagcg agcagtggtt
     7021 gctgaaacga gtaaccctac aacacccatc gaataattat agactttcca tagccatgtg
     7081 gagacagatt caggccattg gtaaccaaaa atctcaggaa ccgcagcaat caaaatgaaa
     7141 atgcttgaaa ataaaatagc tggcatcgct gttaaaaatc cgtcacgaac ggctcctaaa
     7201 taactattac gtgagatttt ttcaaaaaat ggtttccctt tttcgatttg tttaatgatt
     7261 ccatccatag cgttcttctc ctatccttga ttctgtctgt acagtacaat aaagtcttga
     7321 atcagatctc tcagcaataa agtggtcatt aaatgatcct gcccatggat aaaaatgaat
     7381 cccatctcca tctctttccc agatgcttct tccgctaaaa tttttgtttg cgaattatgt
     7441 gcaagggtta aattttcgtc ggcttctttc aacgccgaat caacatgttc aaagtttcct
     7501 tgtcttactt ctttcaataa tttcaataat gtactgcgag cgtcgcctga atatgcaacg
     7561 atctcaaaac caagcatttg taattcctct ttattcatct tttcttcctc cattatcact
     7621 tattaaataa tcgttttggt ttcactgacc aacttgtacc agtaagctga ttcttttggg
     7681 taacgcttct gggtttcaaa atcaacataa aacaagccat accttttgtt atacccattt
     7741 gtccatgaga aaagatccat aagcgaccac aaaaaatacc ccttcacatt taccccggct
     7801 gttatcgcct tgcttaagga ttccaaatag actctcaaat agtcgattct cggctgatcc
     7861 ataataatgc catcttcaaa ctgatcttta taccccatcc cgttctcagt gatatagatt
     7921 ttgttgtaat gtgggtaatc gcttttaatt cgcagcaaca agtcatatag accttctgga
     7981 taaatcagcc agtcccaatc agttcgagga attccttctt tataaatacg ttctccgatt
     8041 cccttcactt tataaacaga ggtccctttt tcacccgtac cgttatgatg gattgcattt
     8101 tctccatcgt aagctttgac aaaatggcat tggtagtgat tgatccctaa gtagtcatta
     8161 cgcgtggatg cttttttcaa ttcaacgaaa tcttcttcag gaaaatgata gctggcctga
     8221 tttgcttcac atatctcatc aagagcagtc aaggtttcag ttgaataata gcctaaatag
     8281 gtagcatcta ataagaaacg gatcgacaat gcatcgtcta aaaaggcagc atgcttatct
     8341 tccggcgcgt ctgttgccgc atatttcgtc tctaatgagt gaactacacc aatttcaccc
     8401 ggaagttcgt tttctttgaa atagttcact acacgggcat gagcaaccat catattatga
     8461 agacaggcga cgatctttgt aaaatcatat ttgattcctg gtgggaaaac acctaacaaa
     8521 tattgattgg ttgcaactgg atagatttca ttaaaggtac tccacacctt gacttctttg
     8581 aattcatgaa aacaaaaaat ggcataggaa acaaatgctt ctattgtttc tcgattcaaa
     8641 aaatctccat ggtcaaacaa acgtttaggg gtatcaaaat gatgcaacgt cacaaacggc
     8701 gttacgtttc ttttcttaca ttcttcgaac acacgatgat aaaaagcgat tccttctgga
     8761 tttggtttcc cagctccatc aggaaaaatg cggctccaag caatggataa tcttagacca
     8821 tttacaccaa aacgttcaca caattcaatg tcctttgcat attgatgata aaaatcactt
     8881 gccggatcgg gactgaatcg cccttgttcc tctaaaaagt catcccaagc aacagcgcct
     8941 ttgccacctt ccttagtggc tccttcaacc tgataggctg ctgtggctcc gccaaaaata
     9001 aaatcttctg gtaatttaag catgtacatc tctcctcaaa tctataatca gttttcgctc
     9061 tttctaactg attgcattgt aaacgcatcc aaaacatttg tcaacaaaaa caaacacaaa
     9121 taatcataaa aaccagaaaa cagcaataaa atctattgta acaaacataa aaaccaccct
     9181 aaatgtttac ttttgttcga attagattga ctgttgtccg tttatatgat agattatcct
     9241 taaattagag gtgaagacaa tgttgaaaaa agaaagattg cttactatcg tagaaatggt
     9301 caataaaaaa ggaatcctta ccgttaatga aatcatcaat caattagatg tttctgatat
     9361 gaccgttcgc agagatttag atgaattaga aaaatcgggc aagctgctgc gtgtccatgg
     9421 aggggctcaa agtctttcct acactttaga tcaagaactt tctcacactg aaaaagccac
     9481 tctgcagatt caagaaaaaa aagaaattgc tgccacagca gcctccttga ttcacgaggg
     9541 agaaacgatt ttcttaggac caggtacaac aatcgaacta ttggcgacgc atttactgaa
     9601 caagaaaatt cggatcatta caaataatta tccagttttt gatattcttg ctcaagcaga
     9661 tggaccagaa attctactga ttggcggtga tttccggaaa aataccggcg catttgtcgg
     9721 accgatcacc aacgacaatt taagaagact aaagtttaca aaagcattta tcagttcaaa
     9781 tggcgttcac aacgaagcga tctctactta cagtaccgaa gaaggagaag cacagcagat
     9841 cgctctaaat aattcccgta agaaatatct actcgttgat aataaaaaat tcaatcgaga
     9901 tgatttttat gtgttttata atttgcatga tttcgatctg ctcattacgg atcgccagat
     9961 ctcgcgggag gttcaagagc actatgcaca atatgtcgat ctgcaaattg ctgattcaac
    10021 cgaaaatacc actataggag cgtaactatg aaagggatta tatttgattt caacggaaca
    10081 atgtttttgg actctcatct gcatgaagct gcttggctgc atatgataca tgatcataca
    10141 aaagatggtt tatcagacga ggatatttta aaaaatattc atggtcggac gaatacggag
    10201 attttgaaac atttcatttc agagcatttg accgcctctg agatcgccgt attgtcagaa
    10261 gaaaaagaaa gctattatcg tatgctttgc ttgcaaaatg aaaacgagct tcagttgaca
    10321 aaagggcttg aagcgactct tgaccagctg actgaccaac agattccatt aacgattgcc
    10381 acggctactg tcaaagagaa tgttgctttt tattttgata ttttcgattt agcccgctgg
    10441 tttgactggg acaagattgt ctatgacgac ggctctttcc ctgggaaacc gcaaccggat
    10501 atttttctta aagcggcaga caaactgagc ttagctccgg ctgattgtct tgttatcgaa
    10561 gatgcttact caggattact tgctgccaaa cgtgcgggga tcggaacaat tattgcaatt
    10621 gatccattcg gtaaaaacaa aacagtattt gaacaggcac agctaaataa agatggaacc
    10681 atcaaagatt ttactggttt ttggacagag cacctgtctg ccacatccgt taattcttaa
    10741 acgattcaac taactaccaa agaaaagcaa ctgaaatgca cacctccttt tataaaaaac
    10801 tgccagtgaa acgacatgaa agatacactg ctatatcctt agtgaaatat agcagtgtat
    10861 ctttgacttt ttcattcttt taaccatatg acgcgttcat ctctatctat aattaacatt
    10921 aaaaatagtg ttttcacatt atttctcaaa ccaatcttaa aaatcatcag gagtactttt
    10981 ttgatatact tttattaatg ctgggcgtaa tggttaaaag atttccacta gttcctctcc
    11041 accaagctga gttgaaaaat gcttatctgt aaacaacata gacaaagagg aaataaacgt
    11101 ttccgtatct ttctctgtcc caaggatatt aattaatttt cctactaggt aagtatcatt
    11161 aaacttaaga tctgccttca catcaaaagt tttgataaaa gcttttatca tgcgtacaat
    11221 tttgtcttta tcgccataat tgatttcacc actcatatca atatagtcac taatccctga
    11281 agaaataatg gcaactgctt cttctttcgc cttatcagat aattcattta atgactggga
    11341 ttgattgatg acttcttgag ttttatctgt tacgtaatct ctcattatcc tatcggcata
    11401 ctccgcaaag taatcttgaa aaaattcttc tacagggtaa ccaacttcgt tttcccaacc
    11461 aggagaaatt tcattatcag tacttattaa atctattaaa aaccaacccg ttctctgagt
    11521 gttgattcta taatattttt aatttcctat ataaatcaaa aatattatag aatcaataag
    11581 ctaattaaga aacaaacttt agaaactcta gaagaacttc tgaatttata tagaagtgtg
    11641 tagctactta ctaaatatac aaaagttaaa a
//
LOCUS       DAYYME010000090        11585 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000090 DAYYME010000000
VERSION     DAYYME010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.091"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHBFW_001981"
     CDS             6..305
                     /locus_tag="ACHBFW_001981"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9318282.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHBFW_001982"
     CDS             378..1739
                     /locus_tag="ACHBFW_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFM9318283.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHBFW_001983"
     CDS             1761..2021
                     /locus_tag="ACHBFW_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318284.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHBFW_001984"
     CDS             2035..2787
                     /locus_tag="ACHBFW_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFM9318285.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGKKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHBFW_001985"
     CDS             complement(2881..3486)
                     /locus_tag="ACHBFW_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9318286.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACHBFW_001986"
     CDS             complement(3539..4513)
                     /locus_tag="ACHBFW_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFM9318287.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACHBFW_001987"
     CDS             complement(4891..5610)
                     /locus_tag="ACHBFW_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9318288.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHBFW_001988"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHBFW_001988"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM9318289.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACHBFW_001989"
     CDS             7161..8558
                     /locus_tag="ACHBFW_001989"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9318290.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACHBFW_001990"
     CDS             8639..9079
                     /locus_tag="ACHBFW_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM9318291.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACHBFW_001991"
     CDS             9227..9553
                     /locus_tag="ACHBFW_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9318292.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACHBFW_001992"
     CDS             9554..10303
                     /locus_tag="ACHBFW_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFM9318293.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACHBFW_001993"
     CDS             complement(10383..10838)
                     /locus_tag="ACHBFW_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM9318294.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACHBFW_001994"
     CDS             complement(11265..11540)
                     /locus_tag="ACHBFW_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318295.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         3656 a         2013 c         2353 g         3563 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtaaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcc
//
LOCUS       DAYYME010000091        11530 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000091 DAYYME010000000
VERSION     DAYYME010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.092"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACHBFW_001995"
     CDS             complement(105..629)
                     /locus_tag="ACHBFW_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFM9318296.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACHBFW_001996"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACHBFW_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFM9318297.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACHBFW_001997"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACHBFW_001997"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFM9318298.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACHBFW_001998"
     CDS             complement(2351..3031)
                     /locus_tag="ACHBFW_001998"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM9318299.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACHBFW_001999"
     CDS             3157..4026
                     /locus_tag="ACHBFW_001999"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9318300.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACHBFW_002000"
     CDS             4052..4567
                     /locus_tag="ACHBFW_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFM9318301.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACHBFW_002001"
     CDS             complement(4614..5177)
                     /locus_tag="ACHBFW_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9318302.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACHBFW_002002"
     CDS             complement(5232..5411)
                     /locus_tag="ACHBFW_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318303.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACHBFW_002003"
     CDS             complement(5488..5997)
                     /locus_tag="ACHBFW_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318304.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACHBFW_002004"
     CDS             complement(6093..6473)
                     /locus_tag="ACHBFW_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9318305.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /gene="pnuC"
                     /locus_tag="ACHBFW_002005"
     CDS             6803..7552
                     /gene="pnuC"
                     /locus_tag="ACHBFW_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFM9318306.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="ACHBFW_002006"
     CDS             complement(7736..9013)
                     /locus_tag="ACHBFW_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318307.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="ACHBFW_002007"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="ACHBFW_002007"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM9318308.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="ACHBFW_002008"
     CDS             complement(9935..10879)
                     /locus_tag="ACHBFW_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFM9318309.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11528)
                     /locus_tag="ACHBFW_002009"
     CDS             complement(10881..11528)
                     /locus_tag="ACHBFW_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFM9318310.1"
                     /translation="MLGFAFKLDSILIVMVALVVTALTGGLGIQGMLELLGTSFVNNR
                     GMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGIFRMIFAAFNVSFG
                     GVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCWFFGQVLFIGGAGA
                     LLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKLAKKYYPGKGQ"
BASE COUNT         3275 a         2448 c         2037 g         3770 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaataacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa
//
LOCUS       DAYYME010000092        11298 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000092 DAYYME010000000
VERSION     DAYYME010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.093"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..415
                     /locus_tag="ACHBFW_002010"
     CDS             <1..415
                     /locus_tag="ACHBFW_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFM9318311.1"
                     /translation="NIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYA
                     ISKNSEDEDKIKKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHD
                     MDAFIKSAAQFIAADYDADLADQFINGNNFMPTTN"
     gene            complement(546..1022)
                     /locus_tag="ACHBFW_002011"
     CDS             complement(546..1022)
                     /locus_tag="ACHBFW_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM9318312.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(982..1338)
                     /locus_tag="ACHBFW_002012"
     CDS             complement(982..1338)
                     /locus_tag="ACHBFW_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318313.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(1316..1501)
                     /locus_tag="ACHBFW_002013"
     CDS             complement(1316..1501)
                     /locus_tag="ACHBFW_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318314.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(1502..1774)
                     /locus_tag="ACHBFW_002014"
     CDS             complement(1502..1774)
                     /locus_tag="ACHBFW_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFM9318315.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(1771..1971)
                     /locus_tag="ACHBFW_002015"
     CDS             complement(1771..1971)
                     /locus_tag="ACHBFW_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318316.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            2099..2311
                     /locus_tag="ACHBFW_002016"
     CDS             2099..2311
                     /locus_tag="ACHBFW_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318317.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(2344..2538)
                     /locus_tag="ACHBFW_002017"
     CDS             complement(2344..2538)
                     /locus_tag="ACHBFW_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318318.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(2551..2733)
                     /locus_tag="ACHBFW_002018"
     CDS             complement(2551..2733)
                     /locus_tag="ACHBFW_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318319.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(2730..2870)
                     /locus_tag="ACHBFW_002019"
     CDS             complement(2730..2870)
                     /locus_tag="ACHBFW_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318320.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(3360..3725)
                     /locus_tag="ACHBFW_002020"
     CDS             complement(3360..3725)
                     /locus_tag="ACHBFW_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318321.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(3765..4067)
                     /locus_tag="ACHBFW_002021"
     CDS             complement(3765..4067)
                     /locus_tag="ACHBFW_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM9318322.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(4067..4366)
                     /locus_tag="ACHBFW_002022"
     CDS             complement(4067..4366)
                     /locus_tag="ACHBFW_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318323.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(4359..4523)
                     /locus_tag="ACHBFW_002023"
     CDS             complement(4359..4523)
                     /locus_tag="ACHBFW_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318324.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(4529..4798)
                     /locus_tag="ACHBFW_002024"
     CDS             complement(4529..4798)
                     /locus_tag="ACHBFW_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318325.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(4810..5559)
                     /locus_tag="ACHBFW_002025"
     CDS             complement(4810..5559)
                     /locus_tag="ACHBFW_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9318326.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(5556..6248)
                     /locus_tag="ACHBFW_002026"
     CDS             complement(5556..6248)
                     /locus_tag="ACHBFW_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM9318327.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(6254..6925)
                     /locus_tag="ACHBFW_002027"
     CDS             complement(6254..6925)
                     /locus_tag="ACHBFW_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM9318328.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(6918..7259)
                     /locus_tag="ACHBFW_002028"
     CDS             complement(6918..7259)
                     /locus_tag="ACHBFW_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318329.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(7437..8135)
                     /locus_tag="ACHBFW_002029"
     CDS             complement(7437..8135)
                     /locus_tag="ACHBFW_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFM9318330.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            8222..8692
                     /locus_tag="ACHBFW_002030"
     CDS             8222..8692
                     /locus_tag="ACHBFW_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318331.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(8735..8914)
                     /locus_tag="ACHBFW_002031"
     CDS             complement(8735..8914)
                     /locus_tag="ACHBFW_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318332.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(8901..9239)
                     /locus_tag="ACHBFW_002032"
     CDS             complement(8901..9239)
                     /locus_tag="ACHBFW_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFM9318333.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(9255..9458)
                     /locus_tag="ACHBFW_002033"
     CDS             complement(9255..9458)
                     /locus_tag="ACHBFW_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9318334.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(9473..10249)
                     /locus_tag="ACHBFW_002034"
     CDS             complement(9473..10249)
                     /locus_tag="ACHBFW_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFM9318335.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            10548..10868
                     /locus_tag="ACHBFW_002035"
     CDS             10548..10868
                     /locus_tag="ACHBFW_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9318336.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            10886..>11298
                     /locus_tag="ACHBFW_002036"
     CDS             10886..>11298
                     /locus_tag="ACHBFW_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM9318337.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYS"
BASE COUNT         3200 a         2222 c         1719 g         4157 t
ORIGIN      
        1 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
       61 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
      121 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
      181 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
      241 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
      301 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
      361 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
      421 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
      481 ttttgacact aatagaatat catgctgcta acaatgttta cgaacattta gtgaacagtg
      541 taattttaga ataaagagag ttgttctttg tacttctcgc tattgtaaaa atcaatttcg
      601 ttttcttttt tcaatcggtc agcttgtctg tgttcatact catctaaaaa gttcaatgtc
      661 tttctaattt ctgcatgtcg ctgtctgaca tacgatgagc tatacccaac agattcagca
      721 atatcttcta agctcatttg atcaacatat ttcatcttaa caatttgatt atcgatacca
      781 gaaaagctat ctatcagcaa taacatttct tctttttgct cgatcaacaa ttccaattca
      841 ttttctatct tttggatatt ctcttctaaa gaagaagttc tcgaattctt ttcgatgcgg
      901 acgtttgcta aatcaccaca gacccatcga ttcaattcaa gcttactttt attaagattc
      961 cactttaagt aaagaatctg ctcatctagt tcttggtaat cttttaacca atgaaatctc
     1021 acaaacgcca ccccttatgg tagaatagta ttgcgctgct atctcgaaag gggtagcttt
     1081 tttatttttt cttagccgtt ccattctgga ttgccttttg ttccaaacgt cgttttttct
     1141 tcttaatctt tgattttgtt tttcccatac aattctcctt tccgaaaaca cattttgttc
     1201 gatttttaat actaaataaa agttggccag aatttttcgt tcgtctttac tcatcttttt
     1261 ttgaataaga ttactagcat aaaaatagtt agtaccacga aaatgaacag gtaaatcaat
     1321 tacgttcacc ttcttcatgc cagttatcag ccactagaat ctttgcgtta gtgccgccac
     1381 cgttttcttt tttgtatttt tcaaatgctt tataagcagc tgtttttgat ttatagattt
     1441 ttccggaacc gactgggaac atccatgtgc cctctgtgat agctaagatg tatactttca
     1501 tttaattcac ccctttaatc cccaacacta catatccgga cttctgcatg taatcagtaa
     1561 tataagtgat ctctgctttg tatgagtcac cagtgtaact aaatgttggt ctcgttagtc
     1621 caggtaattc atgtttaatg tattttttat attcacgtag tattaactga tcaccaactt
     1681 tgaagtcacg atcattcttg cggatctcga attgtttgcg tccgctcgtc actgcttcaa
     1741 aatattctgg tagtattttt agttcgtgga tcattctgtt tcctccttca aaattcgctc
     1801 atcatcaaaa agtgaatctc cacaaaatcc ttctcccaaa tatttatcgt tcatgtataa
     1861 gtcaaccaaa tatttgtgat ctgcttctcg atattcatta atattaacga tctttatttt
     1921 gtaaacatta ccttcgtcgc tcactatctt gaattgttct ctgactttca ccaccgttcc
     1981 tcctcttttt gaaaaaattt tagtgttttt gtaagacccc ctaaatcagc ttttttattt
     2041 gatataatac ttgtatcgat taatcaaatc gaaattaaat ttaaaaaggt gatttatcat
     2101 gaatatcaac aatttagtaa aactcgttga acaacgtttt aaagaatcag gattagaaca
     2161 attaaaaaaa gataattctc cagaattatt ttctaatttt accgaagacg acattcctac
     2221 tcttaaacaa ttaaaatatt attctgatta ttggacgcaa actcgtgaga atgaattaaa
     2281 tttagaaaaa ctcattatta aaaagaaata gcaactccag cccacacggc tggtttttta
     2341 gtttcaactt ccctccttac gggtcaaaat catcggttcc ggcataatct gatcatcctt
     2401 tagcaacaaa tcaggatatt ttttccttag cttcttattg ttcgtgtaag ttttcaatag
     2461 ccattggttg gcttcgcttt tgctttctcc agaaaaataa attttccctg tttggatgcc
     2521 tgcaagttga atcatcattt tcacctcaaa tcaaattttt tctgctagat ggagcaaaag
     2581 caatccgaca tcaagaatct tttgacccat tatttcagct actttgctcg gcttaactcc
     2641 ttgagaaaag agcaagcgtg cttgctctat ctcttcgtga gtccaaataa atttggcttc
     2701 ctcaaggata attactggat cagttgctct cattgttcct ctggaaaaat gatgtcattc
     2761 ttatgcttgg accaggtatc tgcatatgga acaaagtatt gtctagctaa ttcgatttga
     2821 tcaattaaag cagtttctga taattcatgg tctgcagcaa tttcattcat actatcgcct
     2881 tcatctaaac agataagacc gttgacgtta tcgattttta catttgtgac tgcttgactt
     2941 tctccacgct cataatattc tacagttgat actgatacat cagcttcgga gtttaattgc
     3001 agaatctgtt actgccggga taatttaata ttcttttcat aattcacctt ttttcctact
     3061 gacatcgaat atttttcatg ttatgattag ccgtgaaagg agagctatat tatgtgttta
     3121 cacatgatgt ccttagctca ttcggttctt tggaagcttt tttggaaata actctcaggt
     3181 tcttgacgca aatctcattt attctttgaa acaaaaaact tccattttcc gagagctttg
     3241 gatcacttca aaagagattt gcatttatat ttctgtgacc ttgagtcgcg taaagaacct
     3301 tacagcaatg gcggcaattc cactttcagg aaaccgcctt ttttcattga tattggtggt
     3361 tagcggacta cccattttct ttcggcagtg ccaaaccaca aatgatctgt gttgatgttt
     3421 aggtagttgt ataccagata agctaaatcc aaagtgcctg catcttcaag acgacaatat
     3481 tccaaaaatt ctcccatagc tggacataaa aatcctggag ctatcatatc ccaacagtta
     3541 atagtgcaaa gaaattcgtt tagcttctga acgttttctt tggtttcacg aattacaaca
     3601 tactccaatt cttctccgct ggcagagcat tccaaaactt caaattgatt aagaaatggt
     3661 agaacatatg cttcaaacat caacattcca tctttctgtt tttgcacttg ttttgcatca
     3721 atcatgtcta tcctcctatt cagttatttc ttccattaat ggaattattt caggtcactg
     3781 gacttaacga aaacaccatt gaccatcttt ccagtccgac ctttgatttc gtcataagca
     3841 aaatccaagc actcgtataa atccatatca ttttgcattg ccaagatgat caaggttact
     3901 actacatcgc caattccgtc tcttaaacca tgttcatctt ttctagctag agaagcggca
     3961 acttccccga tttcttcgac cgtttttaac atttgcttgc tgaaatccgc ttgatccaat
     4021 cccttatctt ttgcccactg ctctactttc gcgattagtt cgtccattat ttctcctcca
     4081 catacctaaa ctgtcttcct tttgaatcaa tccataagct cctagctcta tcccaaataa
     4141 tatttttgct cagaccagta atttcagata actgtttagc ggtacccgtt actagaattc
     4201 gatcaccatg ccagattgca atctttctcg gcgttctccg tttgggcttt tcagtccaca
     4261 ttgatttacc gagcttttgg acttctgcaa ctattttttt atcttcctgc caatcttctg
     4321 aataagtcaa ttcgataatt cgctgcattg ccgctttctt atccatcccg acattctcct
     4381 ttcagtaatt tgagtacttg atcaagtgcg ctctcacgtc cgccatgaaa tgtgttgagc
     4441 cacttgtctt cgtacgacac actttgtctt aaagcttctt gatgcattaa ttcgatctgt
     4501 gctgtaaatg tctttaggtc catcttgttt acacctgctc aagttcatta agatgttttt
     4561 gcaagccttt aacacaatca atgaatagta atttggtata tgctaaattt cttaattgcg
     4621 ttccatcgat atagagtgcg aaatagtatc tgagtttact ccaactcgaa cgatcattct
     4681 taattccttc aattccagct tcttcgagtt gatcatatac gtctctcagg acgtctaatt
     4741 cctcgccctt tttataattc gctatttcat taattagttc tagataatcg atcttcattt
     4801 ttccacctct tagaatggtg cttttgattg tctattagct cgttctagcg cttttttctt
     4861 gagataggct tcttggtcga ttgcccattc aggaagcttc tctcgtcttc ctgtgcgctt
     4921 gtatccactg cttgcgttct taggttcact tttttctttc cttgcccaac ttcgaatagt
     4981 tgccaaatag tttttataag tcttaccaga tgattcacaa tactctgaca gtcgttcgat
     5041 tcgctcttgg taatcattag ggaattctgt tttgagtttc tccatctgct catctgacaa
     5101 aagaacattt ttatactctc cgtatttatg acggacgggc ttagccttcg attttttcga
     5161 aggcggtaac tctcttatat attcttttgt attattaaat gtattattaa tagatgtatt
     5221 attatctttg actttttcgt caataggggt attgcgtttt tcgtcaatag ggtattgatt
     5281 aattcgtagg tacctattga ttatttgatt ggtaccctct ttgtaaatga tttcccgatt
     5341 caagtatcca aacttaatca aatcacttac ccatcgcgat atggtctctt tattcacacc
     5401 atataaatct gcaaagtact cattgcctgc ccaacaaaag cctctttcat tacacaaggc
     5461 cgttatctct ccgtataaca acttagtatt tggtttaagt cttttgtcgt accttacgtt
     5521 ggctggtata atcgcataat aacttcgatg ttctgtcatt tttcccctcc aatatttaac
     5581 tttttgattg ttttctggtt taatttgatc cctttgattt gatatttatt tttgaaatta
     5641 atcacaccta ttttgtgctt ctctgtgtga tggattctgc agagtgctgc aaatgtgtac
     5701 tctgcatgat caacttcttt gcgctttcgt cttcctagcg ctttgtcaaa gtgatcgatg
     5761 tcagctcctg ttttgccaca gatgcagcag actctttttg tgatgcattt gtagaagtaa
     5821 tattcttgat tcgcaggtaa aatctcatag ccttctttga aaggaatatg atgttcaaag
     5881 atgaaatcta agatgatatt tgctaagaca ttggcatcac tcacagttgt attcgattcg
     5941 tctttcaggc ttattttgcg ccctgtgaca ccttcaaaac gaaagtagaa gaattccttc
     6001 cagaagtccg ttggcatgcc tgtatcgata aaaatatcgc ctatcagcgc atagatgaag
     6061 tttcgttgct gtacggtgaa acgtctagga tcaataaaac gaatttcaat gactcgatca
     6121 ccatcgtagc cgtcatacat cgtctttagt cgatcaatgt tcacttcctc attgatggtt
     6181 gcgcttatgt cttttccttt gaactttttc agaaccgctg aatatgaatc gattaatggt
     6241 ttaaacactc atatcacttc ttatctaatt cttttctctt agctgctatt gctcgctcca
     6301 tcaaggcaca ttgctcatag cttaactgtt caatagtttc aacgttatca gctaagagcc
     6361 ctaatttatc tgtctgctca ttaacatatt cgattaaggt tttggtcata tctttaccca
     6421 tctgctcatt gaaagcttct agaatcgtct ctagcatgtt taatttcttt gtatcgattc
     6481 taggtggtgt tggaatatct tccccttgaa atacatataa tcccagtccg tgtagagcca
     6541 atgctttcac aaagcatcgc ttcaatgagt tattgatttg cattgcattt ggtttaacaa
     6601 ctggttggtt tcgataatct aaaacaggaa ataattcggt ttccgtgtgt cctttaaccg
     6661 ttactgagac agatacataa gtcccagttt catccataag aaaaggttta tattcctcaa
     6721 caagaaagtc ttgatgagtt ccagaaacaa ccctgtagtg tttgtactca ttaatagtta
     6781 ccgttgcctg tggatcattc tttttcataa tctcccacgc gtgagcccaa gataaataat
     6841 caaaatttcc ttttttcttg agaattttat ttaacttacg actaaaaagt ttttcaaagt
     6901 tcgttgtccc tttgatttca ctcatcaaat tctgcctcca tttcagcaat gtatttctta
     6961 cctggtccgt aataagagat atcaatcaag ttatctctgt cgtactcttc tagcgcatca
     7021 atcaagccat cttcgatgac gtaaatatat tcaggtttat tcgaatgctt cgatagatgg
     7081 ataagataaa catgatccca aatagttaca tggttgccta aatcatcttg atcccaagct
     7141 agttcttcat tcgtcaaaag atttcgtctg atttttcgac cacttgtttc ctcaattttc
     7201 ggcttgcccc aatcaggatc aatcaaatat tgatctagag tggaaagttc tttttccata
     7261 tgctaaaatc tccttatgat gtgttttctt tgtgactctt tgcttgccgg cggagtcact
     7321 tttttatttg ttgccatgct ttttgcttgt caatatgttg ttggcttagg atgattggtt
     7381 tatagtattt ccaccagcaa ttagcaattg ccgtccctat tcttagcgct tcagctctat
     7441 tcattgtcat caccaaaaag tctctgttgt ctgttcagtt gatcaatttc catacggatc
     7501 gcagtttctg gcaaccacat ttcaataaat gaaacagcat catcgaatct cttacgaggt
     7561 aactcgccat atcttgggat tgaaaaggta cgtttaaatt cagaccaaaa ttttgagaat
     7621 acttttttgc tgatttcttc ataagctcgg ctttcttttc cccctagaac tcccataact
     7681 ttcatatttc ctttttgctt aatttcaaac tcttgttgtc cgctaattcg catagtatct
     7741 ttaagcatgg aaacatcttt tttaacatct ttcatttctt ctaattgata gatcatcata
     7801 tcttcaattg tttgaggaac agtattcttg cgaataacat cttccatttc gttgaatgct
     7861 tcaatgtatt tttgtttgaa gtagatagct ttctttcctg taaaccccat agccagcaag
     7921 aaaaatccat ctctactaat gaagaaaact cgtcgatttc tgccatatga atctggttca
     7981 ttaccttcta caaacatctg tccaaaattg gacacatctt tttttagtgc atcaatatct
     8041 cttaaaacat gttgatgttt tttctcgaag ctttctgcca cttgtaagct cgtagtcaca
     8101 gcttctttat ttttcaaaat tactaattct tgcattattt cttctctcct ttttggtata
     8161 atttagttaa aaactggtgg tgtttatttt gaattttttt cacttaacaa tttttcaatg
     8221 gatgtcaata ataagtttta tattgagtac gtttttaaca gggataaaaa taaaaggaat
     8281 gcgccctcaa ctagaagttg aacttaatgc ttcttacttc gctgcagata tgatttatac
     8341 aaaagtaatc atttctaact attcaacaga accagctatg ttagttaatt tagaactttc
     8401 atccgctaac ttaaaccgca gatggtcagc tactccattt aagaaattaa ttgctaaagg
     8461 aggatcagtc gatgataata gaatatactc tgaagctgta ccgctaaata ttcctcctaa
     8521 aagtgctata tcttgttacc ttgcatttga agtagggaaa attaatttta ataaactcct
     8581 taatcaccaa acaaaaatga tatttactct aaatagaacc caaatccaca aaattgttga
     8641 tatcaaaaat acaaacttcc caatagagaa attagtgaaa gaattaaact gagtaatagt
     8701 aatatacatt ctagtttctc ttttctattc atttttagtc agccccctcg gttggctttt
     8761 tcgctctgta ctcagcttca gcttcatcaa gccccataaa aatccaaacc atgtaaacaa
     8821 tcgttcctat caacgcttgc ctgcttcccc aaagtcctaa agcgtagatg attagcggtg
     8881 cgctgaacac tagcgctcta ttaaattttc ccatccgctt gcctccttaa gatttccgaa
     8941 aaatttgttt ctaaaaattc aagtgtttta cttcttaaaa atagatatgt gtctcttcct
     9001 tcaactggat aatagacaaa tccatttttg tttttctcga tatcgataat atttctatat
     9061 cttgggttct ttaaaactct agaagtaaac caatcatatt ttctgttaat ccgttctagc
     9121 acttctggca acgtcatcca tctaccagta tcatcagctt tttttaactc ctcataatcc
     9181 acttgggaga taattacata gccttctgga attgggattt ttgcttctag atattgcatt
     9241 agctgttcct ccctttacaa ttcgtacata gtgataatcg aatctataat tctgtttgct
     9301 tctgcagaag tctttttacc gtttaaaatt aaagataagt agcttttgct aattccaaat
     9361 ctttcagcaa gcatggtgta agttaagaac tttgaacttt cgacatattc tttgattttt
     9421 tctctatctc gttgagtgat ttctgcaatg tcagtcatac taaaactcct ttctaaccaa
     9481 tttcctctaa atccatttga gggtaatatc cttctttttt tagtaattcg tagataaata
     9541 gacgcccttt ctgtgtccat ttggtattca ttacaatttt agtgccacca tcggctttcg
     9601 ggatctcagt tgtatgagat tttgtgtatc cttggttcat gtgttttttg cacaataacc
     9661 attggttacc gacttttttc tgaataccta gtttatgaag taatttattc atctgttgtg
     9721 gagacatccc ataatctgct gcaatctgac taattgttac tgaatctgta gaagataata
     9781 tgctatctaa ataggagatt ttaggttcgt actctgcaat cttttgttct gcgattaatc
     9841 ttccagttct ttcttctttt agttgagttg ctaattgaat gattgtatct ggattaagca
     9901 aagcttcttc tactttttct ggagttagat aacctccatg ttttctaatt gctggcaaca
     9961 cttcacttgt tacccaacgt ttaaattttt tggcagaggg aagttttgat tttaagatta
    10021 aactgtacaa accagattcg ttgatgattg tcatctctct tgattgacct gaggtcgtga
    10081 ttcgcgaccc catcttatct tccaaatcta catgccgcga caaagcatct ttagtgtttg
    10141 aataacccaa aacactcgca acgtctttgc ctacaaaata tggttcatca tttactaaaa
    10201 ttgtccgaac ttcgttttgt tcgaaattaa aaatttgcgg tgtgttcata tttctcattc
    10261 ctttctttta tattcgtaaa caaatttaac aacttttttc taaattcaat tgacagtatc
    10321 tgagtaatac tctatactaa gagcataatt aaataagaca taaaaacatt gattttaaag
    10381 ctttcttggc ggtctgcatt tacatatcaa tagtgttttt tgttgtcttt ttagttgtta
    10441 aatctgttta caagatatag tatagctcat tactcagaat tagtcaaatt atttctgcgt
    10501 atttttctaa actttttttg taaacattta gaaaggttgt tttatcgatg aatacctatg
    10561 aaataataaa agacttggcg aagaggaaaa agatatctat acggcagtta gaaattaatt
    10621 ttggatattc taacggttat ctagcaaagt ggaaaaataa tactcctaat tcagatgaat
    10681 taagtcggat tgctgactat ttcgatgtct ctgtagacta ccttctaggt agagaagaaa
    10741 gagaaacccc taaacatgtg gatttatcag aagacgatac tgttttttct tttgatggaa
    10801 aagaaatatc taaggagaca atgcgtaaag cgattgcaat tgctaaagct ttagaggaaa
    10861 atgaatagtt ggagtgatgg tttgtatgta tttgaaattg aaagaaatgt tgagcgaata
    10921 caatttgaaa gtagtttata tggagatgaa agagcctggt ttctactatc caaaacccag
    10981 aatcattttt ttgaatgaaa atctatacgg tgaaactgca gaagcctttc atttatctca
    11041 tgaacttgca catttcagcg cctctcattt tgaattttca gttttgtacg atacttctac
    11101 aacttttcac tcaaaatttg aaacagaagc agataaaata gctattctaa ttttattaaa
    11161 tatctatatt gagaacgaac tgactgatga atctcagttt aatctcgaaa aatttatgga
    11221 gtattattct attcagaata agctcagata cacttgttat gctgtctgcc aatgctattt
    11281 taagaaaaaa tattctta
//
LOCUS       DAYYME010000093        11281 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000093 DAYYME010000000
VERSION     DAYYME010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.094"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..709)
                     /locus_tag="ACHBFW_002037"
     CDS             complement(47..709)
                     /locus_tag="ACHBFW_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9318338.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(722..2224)
                     /locus_tag="ACHBFW_002038"
     CDS             complement(722..2224)
                     /locus_tag="ACHBFW_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9318339.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            2772..4451
                     /locus_tag="ACHBFW_002039"
     CDS             2772..4451
                     /locus_tag="ACHBFW_002039"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM9318340.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            4578..5057
                     /locus_tag="ACHBFW_002040"
     CDS             4578..5057
                     /locus_tag="ACHBFW_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFM9318341.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            5276..5956
                     /locus_tag="ACHBFW_002041"
     CDS             5276..5956
                     /locus_tag="ACHBFW_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFM9318342.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            5964..7295
                     /locus_tag="ACHBFW_002042"
     CDS             5964..7295
                     /locus_tag="ACHBFW_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFM9318343.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            7310..8272
                     /locus_tag="ACHBFW_002043"
     CDS             7310..8272
                     /locus_tag="ACHBFW_002043"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFM9318344.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            8383..8721
                     /locus_tag="ACHBFW_002044"
     CDS             8383..8721
                     /locus_tag="ACHBFW_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFM9318345.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            8800..9507
                     /locus_tag="ACHBFW_002045"
     CDS             8800..9507
                     /locus_tag="ACHBFW_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFM9318346.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(9610..11091)
                     /locus_tag="ACHBFW_002046"
     CDS             complement(9610..11091)
                     /locus_tag="ACHBFW_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9318347.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         3566 a         1955 c         2353 g         3407 t
ORIGIN      
        1 gagacagata tggttcggat tttttatttg atcattatcc tgttttttaa acttcgattt
       61 tttgaaattt tttatttttt agttcaaaga cttcatcaca gctttttgct acttcaatct
      121 catgagtcac gatgataata cagcgatttt gttcatgggc gatttcttgg aaaagtttga
      181 cgatatcttg tgaagttgtt tcatctagat ttccagtagg ctcatccgct acgattagtt
      241 gatgttcaca gcagatagca cgaacgatag cgactcgctg ctgctgacct cccgaaagtt
      301 tggtcacctg tttttttgct aatatttcat caattcctac acgagccagt cgatctaaag
      361 caacttgtcg ataattctct tgcttggtct tagtgatttc cattgctgct atgacgttgt
      421 ctagagccga taaataagga agcaagttgt aagcttggaa aataatcgag acatcatttt
      481 tacgataact tcttaacccg attttgttca gtgaacgatc ttgatagtat acctttcctt
      541 cttttggtgc atctagtcca gaaataagcg aaagaaaagt cgtttttcca gaaccacttg
      601 ctcctaaaat agcgtacatt tttccagctt cgaaattcag ttctacatct ttataaagcg
      661 catcttgcgg atcttgatac caaaatccca attgttctgt ccgtaacatc aaattcctcc
      721 tttagtttaa taggattttt cttgggttta atcttaatat ccctatagaa gctaaaaaga
      781 cagaaaacag actgattcca aatgctactc cggctaatag tcccaattgt gcaggtttga
      841 cattgatttc caattcgctg acttgatcag aaacagcaaa tggattattc gtggttcctt
      901 gttgaccatc gcgttgaccg ccttgttctt gtctgttacc tggcatttgt ccaccgccag
      961 gagcttgctg gttttgtgtc gtggtttctg tctgttggtc aagtagctgc tgtcccacgg
     1021 cattcgcgac gacgtttccg ctaagcgaag caactcctaa tgcaagaatc atacaaatag
     1081 ccacttcggt aaacaattgg agaatgattt tacttctgga ttctcctaaa gatagcaaga
     1141 cgcctaactc atgccgacgc tcccgaatgg taatcataat gatcagagta agtatgataa
     1201 taccggctac agccactagt aaaacaacat ttttagaaaa acttgccacg ttgtttaaag
     1261 gttccaacat tgactgatac atggaatcat ttgattgtaa gctaaatgta tctgtgtcga
     1321 tcagcttctc agcttctttg agaaactcat ccattttatc tggatcagat agagtgtaaa
     1381 tagcagaatc tatcgtgtta tcgtcagatg tcccatttag ttcatttgca aaagtgtaag
     1441 aagtgtaaag tgtattcgac ggattcatga agttgaattt catacccata ctgccgcttg
     1501 tttcgctgga ttcataaatt cctttgatcg tcatttcata agtggtatct tcatcttcag
     1561 tactcgtgat tgtgaaagta tctccgactg ccaggtcatt tgtttctgct agagtagaat
     1621 caatgactac attgtttgtg cctttgtcgc tgtcatctat tccttcacca tccgtgattt
     1681 ttgctgttcc atcagaaaaa ttactatttt gtgcagttgc tgacacacca ctgatttgaa
     1741 agtctgcttg agtcatttgc ggattcccgc ccattcctcc agcaggttca ccgcctgcat
     1801 cagtagtttc tgaactgtct tcacttgaat cagaactagt gatggcagtt attccatctt
     1861 ttgccatagc tgaggcggaa ctttcaaagg aataactctt tacattatct aattcagcta
     1921 ttttttccgc gtccgttaag gaaactggtg taagagaaaa actgcctgga tctggacgtt
     1981 cttcagtggt agaaccgctt gaagaagttt gttcctgttt tttaaatgaa gcttctcgat
     2041 tggcacttaa agtcactgtt gctccaactt ctttttgtgc ttgattcgct gcttcttctg
     2101 cagcgcttcg aatcgtgagg ccagctagca caaaaataag aatggctgtg aacacggcaa
     2161 acaataacaa tgaacgtccc cattttactt ttgtactttt ccatgcacgt ttcataaaat
     2221 tcatattttg catccctcct agtagatgca tttgataata gacaacctat cttaaagcta
     2281 gcttaaatct tatgctgttt gcacaccatc aacatgaggt tctgtatata taaaaagtca
     2341 ctatagttat tgcaaaaaat gtggtaaaat gaaaaaagta tgtttcaaac cgacgaagaa
     2401 gaaatgaaag aggaatagca aaagaaatcc tagatgctac agagggattt ttacgtgcat
     2461 agttgttcta acaattgctg atactagcga tttagtttat ttgttgtata ataatgggga
     2521 acagctgaat acttatataa gtattctggc tatagcgttt cgcaaaagat ccattttgtt
     2581 aatgaagtat atccaaaata aaatgcccat ccaactgtca gaacgcggat gtgctggtaa
     2641 ttttcctgct gattctccat aagttgagaa atagtgattt tgcatatgct tcattggaat
     2701 ggctcatata tttagataaa gaagaaaatc gacatcttta aacttggatg tcataagaag
     2761 aggtgaagtt tgtgagtacc attaaaatta tcccattagg cggcgttcgt gaaaatggaa
     2821 aaaacatgta tgtcgcagaa gtgggcgagg atatttttat tttagattgt gggctaaaat
     2881 atcctgaaaa tgagttgttg gggatcgacg tcgtaatccc tgattttacg tatttagaag
     2941 aaaacattga tcgtgtagct ggtgtctttt taacacatgg acatgcagat gcgatcgggg
     3001 cacttcctta cttgttgtca aaaatttctg taccagtatt cgggacagag ttgacgatcg
     3061 agttagcaaa attgaatgtc agtcgtaatg aagcagccaa aggatttaag gatttccatg
     3121 tggtagatga gcatacagaa atagactttg gtgaaacagt agtgagcttt ttccgtacca
     3181 ctcatacgat accagattca gtggggatca atctgaaaac aaaagaaggt agtatcgttt
     3241 atacgggaga ttttaaattt gaccaaacgg ctatcccaat gtatcaaaca gattatgcac
     3301 gattggcgga aatcggcaaa gaaggagtct tagccttact aagtgaatcg gcaaatgcag
     3361 aaaatcctca gcctgtagct tctgaagccc aaatagcaga tgaagtattt gatacgatca
     3421 aatattggga agggcgtatc atcgtcgctt gtgtagcaag taatttgcaa cgagtgcaac
     3481 aagtattgga tgcagcagaa aaagcacaac gaaaagttgt cttgactggt caagatttcg
     3541 aacgaattat tcgcacagcg atgcgtttag aaaaacttca attaccaagt gaagatctgc
     3601 ttgttacgtt gaaagacatg aaaaactatg atccagaaga actgatcatt ttagaaactg
     3661 gccgaatggg tgaacccatc aagtctctgc aaaaaatggc aaacggtaca caccgtaatg
     3721 tcaagattga agaaggagat ttggtttata tcactactac gccaagcatc gcgatggaaa
     3781 ccatcgtggc gaaaacggaa gatatcattt atcgagctgg tggaacagtg aagttgatct
     3841 ctgagaatat gcgcgtttct ggccatgcca atcctaatga cttgcaatta atgatcaatt
     3901 tgatgaaacc aacatatttt gtgccagttc aaggagagta tcgtgaatta gctgctcatg
     3961 cagatttggc tcatgcagta gggatgccat acaaaaatat ctatattact ggacgcggag
     4021 acgtgctgga atataagaac aagcgtatga gtgttgcagg aactacaagt gcagaaaatg
     4081 taatgattga tggattaggc ataggtgata tcggaaacat tgttttacgt gatcgaaaaa
     4141 tcttgtcaga agacgggatt ttcgtagcgg ttgtcactat cagtcggcga gaaaagaaaa
     4201 tcatttcaaa acctcaaatt acttcacggg gattcgttta cgtaaaagct agccgtgatc
     4261 tgatgaaaga aagcagcaat atgattgaaa caatcgttga gaaacattta gaaagcaatg
     4321 attttgagtg gagcaaatta aagcaagaca tccgagatca attaggccgt tacttatttg
     4381 aacaaacaaa acgccgccca gtgatcttgc cagtcattat ggaagctact caacgtcgaa
     4441 ataaaaaata aacaaaaaga gtaagatgaa cctctctata gagtgctcgt cttgctcttt
     4501 ttttgcgaaa aacaaataat ttttgaatac agcatccctc gctttataat aaacgataga
     4561 ataaagggag tgaatcaatg atgagtcaaa caaaagtcga acgttattta gaagaacaaa
     4621 agatcagcta tcaaccactt gattttagtc atgtggtaga agaatctttc acagaagaac
     4681 tacaaaaaag agggattgat ccagctttga tatgtaaaac tctcgtagtg aaaggaaaca
     4741 aaactggggt gatcatcgca ttgattcctt tgaacgatca cttagattac aaaaaaacga
     4801 gacaagtgac gaaagatcgt aaagttggtt ttcctggaat ggattttgta ttaatgcata
     4861 caggatatcc ccacggagca aacaccccta ttggaataaa actcgcacat cctgattatt
     4921 gttttttagc agacagttct ttaaaagaaa aagaagaagt aattgtgtca agcggtgaaa
     4981 tcgggagaag ccttcggata aaagtagccg atttagaaaa gatcgtccat cctatttacg
     5041 ctgatttaat tagttaaggg acgagaagag aagctaagca gagcagagac ctcaaacttg
     5101 agattatatc tgcttggtac ttggtgcatc ctgtattatc tgttacaatt gctttgtaca
     5161 tactgtacta tcaatccaat gaaaatatat ttagcagcta tattttttca ttgatgtgca
     5221 agtgatgaaa tcgtttttaa taaaaaagat acagagaaaa cgcgaggtgt aaaatatgaa
     5281 aattacgtat catggacatt cctgtttaac aattgaaatg gaaaatggcc aacgcgtgat
     5341 gattgacccg tttattacag gaaatccatt gacagactta actgcagaag aagtaaagac
     5401 agattggttg ctgattactc atggtcatag tgaccacata ggagatatga ttcctattgc
     5461 aaaagaaaat caagccacaa ttatcagtat tgttgagatt gctgattatg cgaacagcag
     5521 aggagtagat tcattcggta tgaatatcgg tggaaaacat gcgtttccat tcggcgtggt
     5581 aaaattcgta catgcacagc atagttcaag ctatgaagta gacggtatcg ttcagtatat
     5641 gggcgagcct agcggaatta ttattcaagc agaaggaaaa acgatttatc atgcagggga
     5701 tactgcttat ttcagtgatc taggattgct tgcagaagaa ttcgatattg atgtcgcttt
     5761 cttgccgatt ggcgataact atacgatggg gccagaagat gcagcaaagg ctgcgcaaac
     5821 aattcgtgcc aagaaagtag ttccaatcca ttacaatact ttccctgtca ttgaacaaga
     5881 tcccgaatct tttgtagagt tactggaaga tagtgaagga aaaataatga gcgtctcgga
     5941 aacgctggaa gtgtaggcag aaaatggcga caaaacatga tttgatttta gaatatattg
     6001 aaagtttacc tgttggcaat cgaatttctg taagaagcat cgcaaaagaa ttgaatgtca
     6061 gtgaaggaac agcgtaccgt gcaatcaaag atgcagaaaa tgtcggacta gtttcaacaa
     6121 ttcaacgagt agggaccatc cgtatcgaac gcaaattaaa aaaacatatt gaacgtttga
     6181 catttggcga ggtcgtcagg atcattgaag gggacgtttt aggcggggcg gcaggattag
     6241 acaaggtctt gaataaattc gtcatcggcg cgatgactaa acatgccatg gaacgctata
     6301 ttacgccagg atcactaatg atcgtcggca accgggcgga agtccaaaaa ttagcattgg
     6361 aagatggcgc cgcagtattg atcacaggag gattcaatac gactgctgag attgcaaagc
     6421 tagctgatga tcttgaaatg ccgatcttac gcacctctta tgatacattt acagtcgcaa
     6481 ccatgatcaa ccgagcgtta agcgaccaat tgatcaaaaa agatatcatg cttgtcagtg
     6541 atatctatat gccagctgaa aagactcatt atttgcagca gacagatacg atttctgatt
     6601 atcagcgttt gtcagaagaa acgcagcatt cgcgttttcc agtcgtcaac cgccatcatc
     6661 gtttgatggg gattgttacg gcaaaagatg ttttagggaa atcgccaaac cagatcatcg
     6721 atcgggtgat gacgaaagaa ccaatcagtg taaagaaaac aatgagtatt gcctctgtta
     6781 gccatcaaat gatctgggat gggctagaag tcatgccggt cgtttccgat gacttgacat
     6841 tggaaggctt gattacccgg caagatgtaa tgaaagcgat gcagcttgtc cagcgacaac
     6901 cgcagattgc agatacgatt tcggatcaga tatctggaaa tatccatacg attgatactg
     6961 accgtgaagg aaatcgtttg gaaaacccta agttcaaatt cgtagtagca ccacaaatgg
     7021 tcaatggcgt tggaaccatt tcttttggcg tacttagcga aatcatttct gatgtcgcac
     7081 aaaaaacaat ggtgatgaac caaaaacgaa atatcttgat tgaacaagtg aacctacatt
     7141 atttgcggct gatccaattg gaaagcgaat tagacattcg tccacgtgta ttggaaatcg
     7201 gcagacgttc cgctaaactg gatatcgaag tgtttattga aaacaccatc gtttccaaag
     7261 caatcgttgt ttgtcaagta atggaacgtt cttagaaagc aggagtgaaa tgacagtaca
     7321 gcaagaaata gtagatacaa taaaaaaata cgataaaatc attatccaca ggcatttgcg
     7381 ccctgatcct gatgcattag gatcacaagt aggattagcc gaactattac gggaaagttt
     7441 cccagaaaaa gaaatacgtc aagtaggtga aacgattgaa gggcttcgct ttttagcaga
     7501 aatgcaagaa gttgaagatg tcttctacca aggagcgcta gtcatcgtga ctgatacagc
     7561 taattcaccc agaatcagtg atcaaagata tcagttggca gaaaaactga tcaaaatcga
     7621 ccatcatccc aatgatgacc cttatggaga tcttgtgtgg gtaaatacaa aagcaagtag
     7681 ctgtagtgag atgattgcag aatttgcttt gatgtttcct gaatctttaa agatgaacgt
     7741 taaggctgca cggttacttt atgcaggaat cgtcggagat acagggcgct ttttgtatcc
     7801 ctccacgacg tcccgtacat tagagattgc ttcgatattg agaaattatc catttgatgc
     7861 aagtgctttg aacagagaga ttgaacaaat gccgatgaaa gtcgctaaac tttccggtta
     7921 tctttatcaa aatatccagg tggatgaaaa tggtgcagga agaataatcc ttccgcaaga
     7981 aattcttgat aaatatggaa tcgatgattc agagacagct tcgatcgttt cacttcctgg
     8041 agtgattgac gaagtgctgg cttgggggat ttttgttcag cagccttcag ggtattaccg
     8101 cgtacgttta cgttctaagg ggccgatcat caatgaattg gctaaacgcc atcatggagg
     8161 aggccatcca ttagcaagtg gtgcgaatgc aaaagattta gcagaagtag aggaaatcta
     8221 taaagagatc caagatatat gccaatcgta taagagcaga aaaacagaat gatacttcgt
     8281 tcgaaaacgt gccttttgta gtaaaaatca caaatgtatc ttttcaagat cttctttttc
     8341 cgttacaata gtgctcacta aagattttaa aggagaaaga agatgacatt accaaattgc
     8401 ccagaatgtg gctcagagta cacatatgaa gatcgaggct tgttgatttg cccagaatgc
     8461 gggaacgaat ggaacaaaga agaacaagaa actgtcatag aagacggatt agtggtatta
     8521 gatgcaaatg gaaaccgtct gttagagggc gatagcgtga cagtgattaa agacttgaaa
     8581 gtaaaaggtg cgtcaggtgc cattaaacaa ggaacaaaag tcaagaacat ccgtttagta
     8641 gaaggcgatc ataatatcga ttgcaaggta gaaggatttg gaccgatgaa actgaaatcg
     8701 gaatttgtga aaaagaatta aagaatattc aaggtctttt cacattcttt tcctactact
     8761 aatgataaaa taaagtaaac gaaaaggagt gaaacgaaaa tgtatggtta tggaatgtat
     8821 ggttttttag atccgacatt tcttttggtc attcttggat tagttatttc cggaattgcg
     8881 tcagcgtacg tcaatagtac tttccgaaaa tacgatcaag tgagaagcaa aaacaatgtg
     8941 actggtacac aagcagcaca atatatatta caaagtcaac agataaataa tgtcggtgtt
     9001 caacaaattg ccggagatct cacagataac tacaattcag gtaataagat gttgagtttg
     9061 tctcaagcaa ctgcacagtc tacttcagtt gctgcgatag gtgtagcagc tcatgaatgc
     9121 ggacatgcgg tacaagatgc ttcgggttat gtcccattga agattcgtgc agcacttgtt
     9181 ccagcagcta actttggttc gatgatttcg ttccccttga ttatggtagg tgtattgctt
     9241 agctggaact ctacgttaat caatatcggg attttcgctt tttctttagc attacttttc
     9301 caattggtga cattaccagt cgaatttaac gcgtctcggc gagcaatcca aattttgagt
     9361 gaaggcggac tactgacgga agaagaagta ccgatggcaa gacacgtatt atttgcagca
     9421 gcactgactt atgttgcagc agcgctttcg acattcctgc agctgttgcg cttgatctta
     9481 ttgttcggcg gcaaccgaag agattaaaat aaagaagttg tgaaatcagc gctttgatct
     9541 gagctaatac cgagagttag ctgattgaat accatttatt tggaataaga ggatgtgaca
     9601 agaattttgt cacatcctct ttgttttgtt ctcataacct tttttcatat acagttcatg
     9661 aaccgctttt attaacttgc caaataacac tgtattgtgc gttgtatcca gatagaaagt
     9721 ctccgtttca tctgaaattt tctcatgctg atctgtaatg atcaaatcag catgttctga
     9781 atatgcggta attttaacag catctttgcg aaataactgc tgcaaatggc tttgaagatg
     9841 ataatttcct gtaaactgtt tggttatctg caaatggatg agtaactgct ctttcgtttc
     9901 tgtttgtagt aagtgttgga tcaattgact aaggtattga ctgatttggt ttctttcccc
     9961 attttttgaa aaaattgtat cgattttttc tcttaatttc ttttcctgta taaaagaact
    10021 agaatgattg gacgtggctt ctggaaagca aagtgtagaa aaactactgc tggattcacc
    10081 caacagttcg aaaaaaacaa ctgccaacac aagaaaataa aaataaattg gtattttatt
    10141 ttctaaatgg ttgagaggaa actgttgagt taagtaagtc atcaattgac cgacttgtcg
    10201 acaaataagg tttttgcttg tggaaaataa aaaaccaatc tcctgttttt gtcgaaaaga
    10261 gatttgattt ggaaaaaaac aatagaaaaa ataattaaag taggctctct ctgtttctgt
    10321 ctcttcacaa gggcgactca ctgaaaaaga agtaaagtat tctgaataat catgtgtatg
    10381 ctgcaataag tccatcaatt cagtgatttc agaagaagtt tttggaaaat atgcctgttg
    10441 tttgtttcgt aatatgaata aagttaacag caagtagata tttttttctt gttttgaaat
    10501 tttttctatt tgctcatctg tactgttcat ttttagtgca tttaattcaa gtgaggaaaa
    10561 tggccaatcg ttacctccaa atgtatctga aagaaaaaag taaagataga gacggataga
    10621 taactcattt ccttcaagag tacagcaatt ttttacagat aggatccgta aatggaattt
    10681 tattaaggaa tgattgagct gttttgtcag tttttcgaga taggagggcg atatataaag
    10741 ttgttcaagt aaatattttt ttggtacagc agtttgtgga taattgatta gtaacatcaa
    10801 atagttaaaa cgattggatt cttttaggta gcataatttt aacttagaaa aaacagctaa
    10861 ctttttagtt tgatcggttt ctttggtttg gatctctcct ttatgattag aataaataaa
    10921 ttcatcaatc aataaaaatt gcttgagtga tacatttatt tttcggatca gttctttgat
    10981 tgtccgtttt ggaatatgta agtcattgga taattcttgt aaagaacaat tatttttttg
    11041 aatgaccatt tgtagaacaa gtaactcttt ttttcttgct tcaattagca taaatacctc
    11101 ctttttattc tgtttttgta tatagttaga caaaaactgt gagtggaaag tcatttcagt
    11161 ctgtgttatt ctcacagagt gtcgccttct tttttatata acgttattta atttttttgt
    11221 taccttgttt attctattgt ttatttccta gggtattttg tcaagatatt cttgttttta
    11281 a
//
LOCUS       DAYYME010000094        11054 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000094 DAYYME010000000
VERSION     DAYYME010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.095"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1044
                     /locus_tag="ACHBFW_002047"
     CDS             <1..1044
                     /locus_tag="ACHBFW_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM9318348.1"
                     /translation="YEIKENAWDSLPEHLYKRHIDNVLILHGKTSWKAVKDVFPDLSI
                     TKIFEYYGGECTDPLADSFVSLCKKKDFNGIIAVGGGKVADLGKLIAYKTNLPIIILP
                     TLAATCAAYTPLSVVYNEEGVMLRYDMFPKANDLVLIDPKIILHSPREMMIAGIGDTL
                     AKWYESHAIISQLTIKPIEVQVAEFAALRCREILLEKSAEAMEAMDQQKINQAFIDVV
                     ETNILLAGMVGGFGDDYGRTAGAHSIHDAMTVLPDSHQQLHGNKVAYGILVQLMIENK
                     LAEIEHLLPFYHHLNLPTSLADMGLHLTESDYQAVAERACLPDEMIHFMKETITPALV
                     VQKMKELEQVTQG"
     gene            1378..2316
                     /gene="yidC"
                     /locus_tag="ACHBFW_002048"
     CDS             1378..2316
                     /gene="yidC"
                     /locus_tag="ACHBFW_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFM9318349.1"
                     /translation="MNKWKNWLLGSGLVTIMLFLSGCVRMDSNGNPDTSGIIYRILVH
                     PMGQAITYLVENFNWSYGWAVILMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     ATQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESTFYGIDLGNPSLVLVAIAGIAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGIFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTPAEPAKQTKKQVQAPKNNAKNGRNAGKQQKRK"
     gene            complement(2497..2901)
                     /locus_tag="ACHBFW_002049"
     CDS             complement(2497..2901)
                     /locus_tag="ACHBFW_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9318350.1"
                     /translation="MSRQLVLISHGKFCEELKNSTEMIMGPQENIYTIPLLPEDGADT
                     YKERFLSVTAPLKDYVVLCDLLGGTPANTVSKLIMEGETIELYAGMNLPMVIEFINSQ
                     MIGAEPDFIGSGRESIVSVNQVIAGMGDDEEE"
     gene            complement(2917..3726)
                     /locus_tag="ACHBFW_002050"
     CDS             complement(2917..3726)
                     /locus_tag="ACHBFW_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9318351.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDDTPELKEMMKTHTQFFNTSNFFNTIITGIDLAIEEKEGVSSKETVSGMKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPVGILIWILAQVAVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKLLPAGIVGGVYWMLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(3713..4603)
                     /locus_tag="ACHBFW_002051"
     CDS             complement(3713..4603)
                     /locus_tag="ACHBFW_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9318352.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLKAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDQHVETMNYKAIERNFLMGALPWALSRMVPVFLAL
                     AFGGGLVQNVVAVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHISYLILGFT
                     ITALLATVFSNVQLLGTSVASVVKDFPGTFNSLPMLAVALIGLALATISYKNDQKNDQ
                     HPVVSQQSQSDEGEIEDDEI"
     gene            complement(4616..5095)
                     /locus_tag="ACHBFW_002052"
     CDS             complement(4616..5095)
                     /locus_tag="ACHBFW_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM9318353.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNIGRIMVVDDEVATNAIEK
                     SGLKLATPAGVKLSILPIEKAAANILAGKYDSQRVLIVAKKPDRILALVEKGVPIKEL
                     NVGNMSQTDETRSITKSINVVDADVEVFKKLDQLGVHLIAQMVPNDKAEDFMSLLIK"
     gene            complement(5112..6878)
                     /locus_tag="ACHBFW_002053"
     CDS             complement(5112..6878)
                     /locus_tag="ACHBFW_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HFM9318354.1"
                     /translation="MTRFEIKEEFLLDGQPFKILSGAIHYFRVHPTNWERSLHNLKAL
                     GFNTVETYVPWNLHEKEEGTYDFSKILDLSHFLELAQSLGLYAIVRPAPYICAEWEFG
                     GLPAWLLTKECRIRSADPRFMKYVKRYYQTLLPKIVPHLVTHGGNVLMVQVENEYGSY
                     GEEKAYLRQVKKYLEEGGIDVPLFTSDGPWQATLRAGILIDDDVFTTGNFGSKAKENF
                     ADMAQFFEAHGKKWPLMCMEFWDGWFNRWKEPIIKRDPEELADAVKEALQIGSINLYM
                     FHGGTNFGFMNGCSARGAIDLPQVTSYDYDAPLDEQGNPTEKYFALKRMMAENFPEMQ
                     QMEPWIKETIVKKGINLKEKVSFFATLDTISKPIHSTYPQTMEELGQNTGYLLYRTTI
                     KKDAVNEKLRIIDGRDRVHVFLDEEKQAIQYQTEIGETIPLTLEKEDHQIDLLFENMG
                     RVNYGHKLLADTQRKGIRTGVMSDLHFITEWTQYCLPLETTEHIDFSKKWEAGQPAFY
                     RFEAELSEIGDTYLDLSEFGKGIVWVNGTNIGRFWEVGPILSLFVPEGLFRKGQNEIV
                     IFETEGRFSEVIDFVKEPIEKS"
     gene            complement(6924..7865)
                     /locus_tag="ACHBFW_002054"
     CDS             complement(6924..7865)
                     /locus_tag="ACHBFW_002054"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFM9318355.1"
                     /translation="MITTVTMNPSIDIAYTLDELRIDQVNRVKTVNKTAGGKGLNVAR
                     VLHLLADDVLATGLVGGQHGAYIKEQLDQSGITHQFLPIKGETRDSIAILHGVNQTEL
                     LESGPVVTEEEQHKFTEHFARLMETTDIFTISGSLPQGFSKSYYAELLALTNKKGTRV
                     LLDTSGQTLLEVLEGKEKPFLIKPNLEELGELVHQTISSATSLDEIQSLLDHPLFEGI
                     NWIVLSLGAKGAIAKVGRHFYQVTIPKIAVVNPVGSGDSTIAGLAHALSKSYTIEETL
                     AFAMACGMANAQQEKTGWIDPVKVDYFRGEINVRKIR"
     gene            complement(7862..8857)
                     /gene="lacD"
                     /locus_tag="ACHBFW_002055"
     CDS             complement(7862..8857)
                     /gene="lacD"
                     /locus_tag="ACHBFW_002055"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM9318356.1"
                     /translation="MNEEKIAAMKSLSTKEGIISALAIDQRGALKKMIKALGKEPTAQ
                     QITEFKELVSAELTPYASAILLDPEYGLPAAKARHQEAGLLLAYEKTGYDATTPGRLP
                     DLLADWSTLRLKEQTADAIKFLLYYDVDEDPEINHLKHVFIERLGSECAGEKRPFYLE
                     LVSYDAKTNDSTSLEYAKIKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVEGYSDG
                     EIAYSKKEAAAFFKEQSDATPLPFIFLSAGVSTALFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGREGAIEWLRTQGRQNIESLNKVINENATPWFDKVKEDSVTAK"
     gene            complement(8850..10043)
                     /locus_tag="ACHBFW_002056"
     CDS             complement(8850..10043)
                     /locus_tag="ACHBFW_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9318357.1"
                     /translation="MFELSKDKLKELGAEITTREIKQQPQLWKETAEILAQNQEKLAL
                     FFDQIKKAANGKRVRVIFTGAGTSQYVGDTAVPYLNEYGDNDHFIFHSFGTTDIVSSP
                     KEFLFPDEPTLLVSFARSGNSPESVATVKIANEQIKTLFHLTITCAKEGQLAKNAATD
                     ERNYLLLMPEKSNDAGFAMTGSFTCMLLSALWVFDQTAVEKKESYLQQLIRTGEDLIS
                     RDQEIQKLLPKSFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVIGFVNNQPYTRNYDLDILEEIKDDQITTSVIAIAQPGNIDFTGTSLLLE
                     ASISLPDAYLALPMIMTAQIIALDTSIKVANKPDTPSPTGTVNRVVKGVIIHEYVNKG
                     ETKDE"
     gene            complement(10061..10789)
                     /locus_tag="ACHBFW_002057"
     CDS             complement(10061..10789)
                     /locus_tag="ACHBFW_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9318358.1"
                     /translation="MESSSKGKSLYYQLVDVLKERIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELETQGYIYRRHGKGTYVSDLSDKSSDLAGAYSFTEEMRELGRVPNTV
                     ILEFKQMEANKKVAKNLNINLGETVFKLKRLRLADDEPLMVERSYLPAKLFIGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDADPLRIADGAPVLYLSRTTYNVKN
                     EIIEYTDSVARADQFHYKIRHVRK"
BASE COUNT         3162 a         2413 c         1959 g         3520 t
ORIGIN      
        1 tatgaaataa aagaaaacgc atgggattca ctccctgaac acctttacaa aaggcatatt
       61 gacaacgttt tgattctcca tggaaaaaca tcttggaaag cagtaaaaga tgtttttcca
      121 gatttatcaa taacaaaaat ttttgaatat tatggtggag aatgtaccga tccacttgct
      181 gacagttttg tttctttatg caaaaagaag gatttcaatg gtattattgc agtcggcggc
      241 gggaaagtag cagatctagg aaaattgatt gcctataaga caaacttacc aatcatcatc
      301 ttacctacct tagctgcgac gtgcgcagct tatactccac ttagtgtggt ctataatgaa
      361 gaaggagtga tgcttcgcta tgatatgttc cctaaagcaa atgatttagt gttgatcgat
      421 ccaaagatca ttttgcactc ccctcgcgag atgatgattg ccggtattgg tgacacctta
      481 gcaaaatggt atgaatctca cgcaatcatc tctcagttaa ctatcaagcc tatcgaagtc
      541 caagtagcgg aatttgcagc gttacgatgc cgcgaaatcc ttttagagaa aagtgcagag
      601 gcaatggaag cgatggacca gcaaaaaatc aatcaagctt ttatcgatgt cgtggaaaca
      661 aacattttat tggctggtat ggttggtggg tttggcgatg actacggccg aacagctgga
      721 gctcattcca ttcacgatgc gatgaccgta ctccctgata gccatcagca acttcatgga
      781 aacaaagttg catacggtat ccttgtccag ctgatgatcg aaaataaatt agcagaaatc
      841 gaacacctac tgccgtttta tcatcacttg aacttaccaa cgagtttagc tgatatgggt
      901 ttacatttaa cagaaagtga ctatcaagcc gtagctgaac gtgcatgttt acctgatgaa
      961 atgatccact tcatgaaaga aacgatcaca ccagcactgg ttgtccagaa aatgaaagag
     1021 ttagaacaag tgacacaggg atagttagcc ccttatacca tggcagtcag cttttttatg
     1081 gatcggctca atggtaaaaa aaaagaagtg gaaaaaattg acattccact tctttttttt
     1141 gcgctaaaat tatgatttca aggaaatatc cgttgctcat cttattttag agaacggagg
     1201 tgctaagtat gctttatact gaagtatttt tagctggatt cttcttttta acattaagcg
     1261 tcttttttga tcctccaatc ggttatttct atgaaatttc tatgaagttg attgaattat
     1321 gagcgctttt ttagtatagt taagcttgtg taaaaattat aaaagaggaa gtattttatg
     1381 aataaatgga aaaattggtt attgggctct gggcttgtca cgatcatgct tttcttgtcc
     1441 ggttgtgtcc gcatggattc aaatggtaac cctgacactt caggaatcat ttaccgtata
     1501 ttggttcacc caatgggaca agcgattaca tatttagtgg aaaactttaa ttggtcctac
     1561 ggctgggctg ttatcttgat gacagtcatc gttcgtatca tcattttgcc attaggtatt
     1621 agtcaatcga aaaaaacaat gatccaatct gaaaagatgc aagcattgaa accacaagtt
     1681 gaagctacgc aacaaaaatt gaaagctgcg acaactcgag aagaacaaat ggctgcacaa
     1741 gctgaaatgc aacaagttta tcgtgaaaat ggcttaagta tgactggtgg tatcggttgt
     1801 ttaccgcttc tgatccaaat gccgatcttt tctgcgctat atttcactgc tcgctataca
     1861 gaaggcatcc gtgaatcaac cttttacggg atcgacctag gtaaccctag cttagtttta
     1921 gtagcgattg ccggtattgc ttatttactt caaggctata tctcaactat cggtatccct
     1981 gaagaacaaa agaaaacgat gcgtacgatg ttgatcgtat cacccgctat gatcgtcttc
     2041 atgtcgatca gtgccccagc tggtgtcact ctttactggg tagtcggtgg gatctttagt
     2101 tgtctacaaa cattcattac gaatgtcatc atgaaaccaa gattaaaagc gcaagttgcg
     2161 gaagaattaa aacaaaatcc gccaaaacaa gtggtgacgc ctcgtaaaga tgtcacacct
     2221 gctgaacctg cgaagcaaac aaaaaaacaa gtacaagcac caaaaaacaa tgcgaagaat
     2281 ggtcggaatg ctggtaaaca gcaaaagcgt aaatagttat ttgtaactag cttcttcgat
     2341 cgacccagat catttctgat tttatgacga taaaagccaa cgtactgtga aatatagaaa
     2401 actatatttc gcggtacgtt ggctttttta tacacggcct ctttatacat ccgtatttat
     2461 atccagttgt atcaccagtc aagaagttgt gcttgattat tcttcttcat cgtctcccat
     2521 gccagcaatc acttggttca ctgaaacgat actctcacga cccgaaccaa taaaatctgg
     2581 ttctgctcca atcatttgcg aattgatgaa ttcgatgacc atcggcaaat tcatgccagc
     2641 atatagttcg atcgtttcac cttccatgat tagcttggaa acggtatttg caggtgtccc
     2701 acccaacaga tcgcaaagca ctacgtaatc tttcaatggt gctgtaacgg agaggaatct
     2761 ttctttatac gtgtccgcgc catcttcagg caataaaggg atcgtataga tattttcttg
     2821 tggtcccatg atcatttctg tactgttttt tagttcttca caaaatttac catgactaat
     2881 caagactagt tgtctactca tctttccaat tctcccttat ttcgaaatca cacctaatgc
     2941 agataatgct acacatacga tcaatacgat gaatattgct cgagtaggtg tcatattttt
     3001 cttacctaac atccaataaa cgccaccaac gattccagct ggtaaaagtt taggtaagat
     3061 catatcgata tagttttgca tattcaacgt tacatcccca atcgttggtg cccatgagaa
     3121 tttaacattg atcatggttg ctactaacgc tccaaccatg aaaactccta gtaaagtagc
     3181 cgcatctgtc aatgcagtca agcgtgattg catcgtggta actagagaaa tcccttcttt
     3241 gtaggcaaat tctagttgtt tccaacgaaa gaccatgaca gcgacctgag caaggatcca
     3301 gataaggata cctactggat taccatctgt tgccatattt gcggctaaag ctccaaatat
     3361 ggtcgggatc aatgccgcaa atacagaatc accaattgca gcgaaggaac ccatcaatcc
     3421 tgctttcatc ccagaaacag tttcctttga agagactcct tctttttctt cgatagcaag
     3481 atcgatccca gtaatgatcg tgttaaagaa attagacgta ttaaaaaatt gcgtatgtgt
     3541 tttcatcatt tcttttaatt ctggtgtatc atcaccatag atttttctaa gttgtggcaa
     3601 gatagtatac agataacctg aaccttgcat tctttcatag ttccaaccta attggaaagt
     3661 aaacaaactt ctacgattga tctgcttaaa atcttctttg gtcaatttat aattagattt
     3721 cgtcatcttc gatttccccc tcatcagatt gtgattgctg acttactact ggatgttggt
     3781 cattcttttg atcattctta taactgatcg ttgccagtgc tagaccaatc aatgccacag
     3841 caagcatcgg taatgaattg aatgttcctg ggaaatcttt gaccacgcta gccacacttg
     3901 ttcctaacaa ttggacattc gagaacactg ttgctaataa cgctgtgatc gtaaatccta
     3961 aaatcagata agaaatgtgt ttctttactg gtaggtagcg tagtaaaatt gcaaacccaa
     4021 ctgctggtaa gactgcccct gcaacagata agccatctcc tagccatttt aggtcgccgt
     4081 ttaaaacagc cacaacattt tggactaagc caccaccgaa tgctagggcc aagaatacag
     4141 gaaccattct ggaaagtgcc caaggtaatg cccccatcaa gaagttccgt tcaattgctt
     4201 tgtaattcat tgtttccaca tgttgatcga tccgatgtgc aaaatacgta ttcgcaaaac
     4261 gtgccaaaat atctaattgg atcatcaaac ttgctacagg aaccgcgatg gccgcaatcg
     4321 cttgttctgg gttcatccct agagaaatgg agaaagctgt tgctaagatc gttccagagt
     4381 tggcatcgat ttttgatgca ccaccaaatg tccctacccc taaaacagtc aactgcatac
     4441 ttccaccaat gattaaacct gcttttaaat cacccattac taacccagca tataatcctg
     4501 caaaaactgg ggcgctcatt gaagaataaa tctgtaattc atctaaaatt tgataccctg
     4561 catataatgt taataataat atctgccacc attgaatatc catgaatcat tcctcctatt
     4621 tgattaaaag actcataaag tcttctgctt tgtcatttgg caccatttga gcgatcaaat
     4681 gaacgccaag ctgatctaat ttcttgaaga cctctacatc cgcatctacg acattgattg
     4741 attttgtgat ggaacgtgtt tcatctgttt gtgacatatt gccgacattt aattctttga
     4801 tcggtacacc tttttctact agcgctaaga tacgatctgg ttttttcgct acgatcagca
     4861 ctcgttgaga atcatattta cctgctaaaa tatttgcggc tgctttttca ataggtaaaa
     4921 tacttaattt gactccagct ggcgttgcca actttaaccc acttttttcg atcgcatttg
     4981 ttgctacctc atcgtctacg accatgatcc gaccaatatt caactttgtc gtccaaagat
     5041 ttgccacttg tccatgtatc aaacgtccat caattcttac tccaataatt gccatcatcc
     5101 attctccttt tctaactttt ttctattggt tctttgacaa aatctataac ttcactgaat
     5161 ctaccttctg tttcaaagat aacgatttca ttttgtcctt ttctaaataa accctcggga
     5221 acaaacagtg aaagaattgg tcctacttcc caaaaacgac caatattcgt gccattcacc
     5281 caaacgatcc ctttcccaaa ctcactaagg tccaaataag tatcaccgat ttctgacagt
     5341 tccgcttcaa agcggtaaaa tgctggttga cctgcttccc atttttttga aaaatcgatg
     5401 tgttctgtag tctctaaagg taaacaatac tgtgtccatt ctgtgataaa atgaagatcg
     5461 ctcatcactc ctgtacgtat tccttttctt tgcgtatcag ctagcagctt gtgtccatag
     5521 ttgacccgtc ccatattttc aaataataga tcaatttgat ggtcttcttt ctcaagtgtc
     5581 aatggaatgg tctcaccgat ctctgtttgg tattgaatcg cttgcttttc ttcatcgaga
     5641 aaaacatgga cccgatcacg tccgtcgatg atccgtaatt tttcattgac cgcatctttt
     5701 ttgatcgttg ttcgatacaa taggtaccca gtattctgac ccaattcttc cattgtttga
     5761 ggataggtcg agtggatcgg cttgctgatg gtatccagtg ttgcgaaaaa gctgactttt
     5821 tctttcaagt tgattccttt tttgacgatt gtttctttta tccatggttc catttgctgc
     5881 atttctggaa aattttctgc catcattcgt ttcagagcaa aatatttttc cgttggattt
     5941 ccctgctcat ccaatggtgc atcgtaatca taggaagtga cttgcggcaa atcaatcgcg
     6001 cctctggctg aacagccatt cataaaccca aaattggttc cgccatggaa catatagaga
     6061 ttgatactgc cgatctgtaa tgcttctttg actgcatccg ctaattcttc cggatctctt
     6121 ttaatgatcg gctctttcca acgattaaac cagccatccc aaaattccat acacatcaac
     6181 ggccattttt taccatgtgc ttcaaaaaac tgggccatat ctgcaaaatt ttcttttgct
     6241 tttgagccaa aattccctgt cgtaaaaaca tcatcgtcaa tcaaaatgcc tgctcttaat
     6301 gtggcttgcc aaggtccatc agaagtgaat agcggtacat cgattccacc ttcctccaag
     6361 tattttttta cttggcgcag gtatgctttc tcttcaccat acgaaccgta ctcattttct
     6421 acctgcacca ttaagacgtt gccaccatga gtgactagat gaggaacgat tttaggtagt
     6481 agcgtttggt aataccgttt gacatacttc atgaagcgag gatccgctga acgaatacgg
     6541 cactcctttg tcaatagcca agcaggcaag ccaccaaatt cccattctgc acaaatataa
     6601 ggggctggac gtacgattgc gtataatcca agggattgcg ctaactctag aaaatgggaa
     6661 agatccaata ttttcgaaaa atcataggtt ccttcttctt tttcatggag attccaaggc
     6721 acataggttt ccacagtatt aaatccaagc gctttcaaat tgtgcaatga ccgctcccaa
     6781 ttcgtcggat gcactcggaa ataatggatg gcaccagata aaattttgaa aggttgaccg
     6841 tctaacaaaa actcttcttt tatttcaaat ctagtcaagc aaatttcctc ctcgttttta
     6901 gaaaccgctt tctaatttta taatcaacga atctttctga cgttgatttc ccccctaaaa
     6961 taatcgactt tcacaggatc gatccatcct gttttttctt gttgtgcatt cgccatccca
     7021 caggccatcg caaacgcaag tgtttcttca atcgtatagc tctttgatag agcatgtgct
     7081 aagcctgcaa tagtcgaatc tcctgacccc acaggattca ccaccgcgat tttaggaatc
     7141 gtaacctgat agaagtgtct ccctacttta gcgattgctc cttttgcgcc tagcgaaaga
     7201 acgatccaat tgattccttc aaaaagcgga tgatccaata atgattggat ttcatctaac
     7261 gaagtagccg aagaaattgt ttggtggacc aactctccta attcttccaa attaggtttg
     7321 atcaaaaatg gtttttcctt gccttccaag acttcaagca aggtttgacc agaagtatct
     7381 aacaatactc gtgttccttt tttattcgtt agggcaagta actctgcata ataactttta
     7441 gaaaatcctt gaggcagact acccgaaatc gtaaagatat cagttgtttc cattagtcgt
     7501 gcaaaatgct cggtaaattt atgttgctct tcttccgtca caacaggacc gctttccaat
     7561 agttctgttt gattcactcc atgtaaaata gcaatcgaat ctctcgtttc ccctttgata
     7621 ggtaaaaatt gatgcgttat ccctgattga tcgagttgtt ctttgatata tgctccatgc
     7681 tgcccgccga ctaatccagt agctaaaaca tcatctgcta ataaatgaag gacacgagca
     7741 acattcaagc cttttcctcc agccgttttg ttgaccgttt tgacccgatt gacttggtcg
     7801 atccgtaatt catccaacgt gtaagcgatg tcaattgaag gattcatggt aactgtcgtg
     7861 atcattttgc tgtcaccgaa tcttctttta ctttgtcaaa ccaaggggtg gcgttttcat
     7921 ttatcacttt attcaacgat tcgatatttt gtcgcccttg tgttctcaac cactcaatcg
     7981 caccttctct accattttca acatatgctt tgactccatc tttccaagtc gcacgtccgc
     8041 ataacacacc gttgaatgtt gaaccagatt catgagcaaa gatcaatgtt tgttggaaca
     8101 atgcagtcga aacccctgca ctcaagaaga taaatggtaa cggtgttgca tcactttgtt
     8161 ccttgaagaa tgccgctgct tcttttttac tatacgcaat ttcaccatca gaataacctt
     8221 ctacatagtt catattgact ggcacttcta ctttcaatac atccacttta tatttaggat
     8281 cagaaaattc tttcatcatc tcgatcactt tatgcggttt gatcttcgca tattctaatg
     8341 aagtgctgtc attggttttg gcatcatacg aaaccaattc tagataaaac ggacgctttt
     8401 cacctgcaca ttcactgcct aaacgttcga taaatacatg tttcaaatga ttgatctctg
     8461 ggtcttcatc tacatcataa taaagcaaaa acttgatcgc atcggctgtt tgttccttta
     8521 atctaagcgt agaccaatcc gctaataaat caggcagacg tcctggagtt gtcgcatcat
     8581 aacccgtttt ttcataggct aaaagtagtc ctgcttcctg atgtctggct ttagcagctg
     8641 gcaatccata ctctggatct agtaaaatag cagaagcata aggcgttaat tctgctgata
     8701 ctaattcttt gaattcagtg atctgttgcg ccgttggttc ttttcctaac gctttgatca
     8761 tctttttcaa agcaccgcgt tgatcaattg ccaatgcact gatgattcct tcttttgttg
     8821 ataaactctt catagctgca attttttctt cattcatcct tcgtttcccc tttgttcaca
     8881 tattcatgga taatgacacc tttgaccact cgattgactg tacctgtagg cgatggtgta
     8941 tctggtttat tcgctacttt tatcgacgta tcgagcgcaa tgatttgggc tgtcatgatc
     9001 ataggtaagg caagatacgc atcaggtagg ctgatcgaag cttctaacaa aagacttgta
     9061 cctgtgaaat cgatgtttcc tggttgggcg atcgcgatga cactcgtggt aatttggtca
     9121 tcttttattt cttctagaat atctaagtca taatttcttg tatacggttg gttattaaca
     9181 aaaccaataa caagtgtttc gctatttaaa aacgattttg gtccatggcg gaagcccata
     9241 gaagaatcaa agatcgttgc cactttaccg gctgtcaatt ccaatatttt caattgtgct
     9301 tctctcgttg caccagccaa acttcctgaa ccaagatagg cgatacgttc aaatgactta
     9361 ggcaataatt tttggatttc ttgatctcgt gaaattagat cttctccagt tctgatcaat
     9421 tgttgaagat aactttcttt tttctcaacg gcagtttggt cgaataccca taacgcactc
     9481 aacaacatgc acgtaaaact tcctgtcatg gcaaatcctg catcatttga tttttctggc
     9541 atcagtaaaa gataatttcg ttcatctgtc gctgcattct ttgctaactg accttcttta
     9601 gcacaagtaa tcgtcaaatg aaacaatgtc ttgatttgtt cattggcaat cttgactgtt
     9661 gccacacttt ctggactatt tccactacgt gcaaatgaca ccagtaatgt cggttcatcc
     9721 ggaaatagaa attcttttgg tgaagacacg atatctgtcg taccaaagct atgaaaaata
     9781 aagtgatcgt tatcaccgta ttcatttaaa taaggtactg ctgtatcccc gacatattgt
     9841 gatgtgcctg caccagtaaa aatcactcga actcttttgc catttgctgc tttttttatc
     9901 tggtcaaaaa acaatgctaa tttttcttga ttctgtgcta atatctcagc tgtttctttc
     9961 cataattgcg gttgctgttt gatttctcgc gtagttattt ccgcacccaa ctcttttaat
    10021 ttatccttcg atagttcaaa catgttgttt tccctctcaa tcattttcgg acgtggcgga
    10081 tcttataatg aaactggtct gctctcgcta cactatccgt atattcaata atttcatttt
    10141 tcacattata ggtcgttcgg cttagatata ataccggggc accatcagcg atacgaagcg
    10201 gatcagcatc tttcttttga acgatacttg cataaaattc ttcatctgct aattggactt
    10261 gctgttcata atcctgagaa aacacatcat acaatggctt actttcaagt aatggcagat
    10321 caagaccaat aaatagtttt gcaggtaaat aagaacgttc caccatcaat ggttcgtcat
    10381 ccgctaaacg aagtcttttc aatttgaaaa ctgtttcgcc taaattgatg tttagattct
    10441 tggcaacctt tttgtttgct tccatttgct tgaattccaa gatgactgta ttaggcaccc
    10501 gtcccaattc cctcatctct tctgtaaaac tatacgctcc cgccagatca gacgacttat
    10561 ctgatagatc agacacatat gttccttttc cgtgtcggcg ataaatatac ccttgcgtct
    10621 ctaattcttg caaagcaagg cgaaccgttg tccgactcac tccataatgc tgacaaagtt
    10681 ctctttcaga taacaatttg tcatgaggtg tcatctgtgt ttcgatacgt tctttcaata
    10741 cgtcaactag ttgataatat aatgattttc ctttacttga gctctccata aaaatacctc
    10801 ttctttactg gttataacca caacacaaga atacccacat attcctcatt tgtcaaaggg
    10861 tttttggcgt aaaaaagcta ttataacaag gtttcaaggt aatttagaac tggtatcaaa
    10921 agatacctca aaaataccag ttgacaaata aaaaacaaaa aaaagctgaa tcttggcaaa
    10981 gattcagcta aatggtctag agaagtaaag aaaaaatatt ctttatacat ctgtggctat
    11041 ttctactatc gaca
//
LOCUS       DAYYME010000095        10897 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000095 DAYYME010000000
VERSION     DAYYME010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.096"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            181..1440
                     /gene="lysA"
                     /locus_tag="ACHBFW_002058"
     CDS             181..1440
                     /gene="lysA"
                     /locus_tag="ACHBFW_002058"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFM9318359.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1427..2227
                     /gene="dapF"
                     /locus_tag="ACHBFW_002059"
     CDS             1427..2227
                     /gene="dapF"
                     /locus_tag="ACHBFW_002059"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFM9318360.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2230..3111
                     /gene="dapA"
                     /locus_tag="ACHBFW_002060"
     CDS             2230..3111
                     /gene="dapA"
                     /locus_tag="ACHBFW_002060"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFM9318361.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3108..3824
                     /gene="dapB"
                     /locus_tag="ACHBFW_002061"
     CDS             3108..3824
                     /gene="dapB"
                     /locus_tag="ACHBFW_002061"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFM9318362.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3830..4525
                     /gene="dapD"
                     /locus_tag="ACHBFW_002062"
     CDS             3830..4525
                     /gene="dapD"
                     /locus_tag="ACHBFW_002062"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFM9318363.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4541..5542
                     /locus_tag="ACHBFW_002063"
     CDS             4541..5542
                     /locus_tag="ACHBFW_002063"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFM9318364.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5539..6900
                     /locus_tag="ACHBFW_002064"
     CDS             5539..6900
                     /locus_tag="ACHBFW_002064"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFM9318365.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6860..8011
                     /locus_tag="ACHBFW_002065"
     CDS             6860..8011
                     /locus_tag="ACHBFW_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM9318366.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7986..9155
                     /locus_tag="ACHBFW_002066"
     CDS             7986..9155
                     /locus_tag="ACHBFW_002066"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM9318367.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9330..10709
                     /locus_tag="ACHBFW_002067"
     CDS             9330..10709
                     /locus_tag="ACHBFW_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9318368.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         3616 a         1906 c         2433 g         2942 t
ORIGIN      
        1 cttctaatag ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat
       61 atttcgaaat atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca
      121 aatctttatc aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa
      181 atgacagaat tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca
      241 cctctgtatg tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc
      301 tttgtatcga aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt
      361 gagatgctgc atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa
      421 atctataccg ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac
      481 aagacgccag aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat
      541 aatttgatgg aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt
      601 atcatgcttc ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg
      661 catattgatt caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag
      721 ttgataaaag aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa
      781 atttttgatg ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat
      841 tttgaccaac ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat
      901 gatcagccga tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct
      961 ttggaaaaac gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt
     1021 ggagaagctg gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa
     1081 tattactttg tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa
     1141 tacgattgtg atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca
     1201 ggaaaaatgt gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa
     1261 tcgggtgatc ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac
     1321 tacaatcgag cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt
     1381 gttcgcagac aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa
     1441 atataatggc tgcggaaatg attttatctt attagattac gcagaagaca cagattatag
     1501 tcaattagcc gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt
     1561 aaaaatcgat ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg
     1621 cggaaatggc attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca
     1681 ggaattcaac gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc
     1741 tttttattgc agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa
     1801 tcaaacttat ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac
     1861 tagtttattt ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt
     1921 ctggagaaaa agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt
     1981 caactttgta gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt
     2041 aggttggaca cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac
     2101 tgggaaaatt tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc
     2161 tggtgaggat gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga
     2221 ggaatgaaga tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga
     2281 tcagtagatt atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat
     2341 ggattattag ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag
     2401 cagatccttc aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct
     2461 ggaacgaata atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca
     2521 gacgcacttt tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct
     2581 cattttacag caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct
     2641 agaacaggca tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc
     2701 attggtttga aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt
     2761 ggagaagaat tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta
     2821 ggaggaagcg gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta
     2881 gtaaaagata cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt
     2941 gaactgatcc atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca
     3001 ttattagact taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa
     3061 aagaaacaat tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac
     3121 tgattggcag tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata
     3181 cggtccttct tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc
     3241 cagaactgct tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct
     3301 tatcatatca acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag
     3361 aaataaaaga acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca
     3421 tcatggtgat gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg
     3481 aactgattga aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc
     3541 tgcttcaaac aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa
     3601 aaagagaaga aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac
     3661 atgatgtatt gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta
     3721 atcgaatctt tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag
     3781 gttattacaa attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca
     3841 agaaatcatc cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa
     3901 tgtaaccgag ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt
     3961 tgtttttggt gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat
     4021 tgaaatcgaa atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa
     4081 aattaatgct cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa
     4141 tgccgtgatc atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat
     4201 gattgatatg ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg
     4261 tgcaggagct gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga
     4321 agacggcgta ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga
     4381 tgcagtcgtg gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg
     4441 tggaatacct gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt
     4501 gattgctgcg ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg
     4561 tattgtgggg gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga
     4621 ttttccagta ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt
     4681 attcaagaaa aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga
     4741 tttagcgcta ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag
     4801 tcagggacta tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt
     4861 aatcgttcca gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa
     4921 ttgttcaacc attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca
     4981 gcgagtcaat tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga
     5041 tcttcatcgt acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac
     5101 ggtgattccg gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat
     5161 gataaatgag acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt
     5221 acgtgtgcca atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt
     5281 tacgacggaa gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga
     5341 tctagcagca cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt
     5401 tgggaggatc agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga
     5461 taacatacgc aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa
     5521 aatagcggag gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg
     5581 atgtctcatg aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc
     5641 caagtgatcg ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac
     5701 ttactttatt tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa
     5761 aggatttgtg atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa
     5821 gatgaactag cacgcataca gaaacaactg gaagacgaaa agataactga agattattta
     5881 gtaagtagag gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt
     5941 attgacgcaa aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca
     6001 gcacaggaaa tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac
     6061 ggggcgaacc ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc
     6121 gcgatactgg cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga
     6181 atcatgatgg ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt
     6241 agagaactga gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc
     6301 ccagttcaag aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa
     6361 gggacaaaaa tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga
     6421 cgcaaggatt ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt
     6481 atctggaaag caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca
     6541 gggatcgaca atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc
     6601 atcacgaaag aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta
     6661 gctttgatct ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg
     6721 ttatcgatat tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag
     6781 ttgaatctga tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat
     6841 gaagggatgg tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga
     6901 aatcgggttt catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga
     6961 ggccgttatc catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga
     7021 agaaacgatt gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct
     7081 ctcttttgct tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc
     7141 gatgctttta gggttgactg actttataac taaacacatt gaggatctta tttataatgt
     7201 agtactgatc tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc
     7261 aggattgttg gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact
     7321 agctaaggga gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga
     7381 cattatcatt aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct
     7441 ggaggcagct gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga
     7501 gtggaatcat ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc
     7561 tggagaagtt attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt
     7621 gaagagggga ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg
     7681 ctataatgac ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga
     7741 ttcaaacaaa ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata
     7801 tacgcaacaa tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca
     7861 tgatccgacg ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac
     7921 gattcttttt acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt
     7981 aaataatgat gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa
     8041 aaatacgaca attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg
     8101 gagaaccgga ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag
     8161 aagacttttc tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta
     8221 tatttcagca acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag
     8281 ttggtgcaac ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg
     8341 ttcttattcc agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg
     8401 aattgatccc gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac
     8461 gagcatttga acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc
     8521 ctactgggac gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat
     8581 atccggtatt tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata
     8641 tgtccatagc tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac
     8701 atgcgatgac tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac
     8761 taattaaagt ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg
     8821 ctgaagcgct aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac
     8881 gaagagacta tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg
     8941 gtgcatttta cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt
     9001 gtgtagaatt agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag
     9061 aaggagaagg ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct
     9121 gcaaacggat caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa
     9181 tcgttaattt tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg
     9241 aacgcgtttc ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt
     9301 ttaaaagaat attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg
     9361 catctcatgg agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag
     9421 atatcacgct atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt
     9481 atttagaaga caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc
     9541 cgcaatccaa tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa
     9601 aaacagtaac ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat
     9661 tgattttgag ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa
     9721 atgtctactt gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc
     9781 cggctattaa aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag
     9841 caagccgaaa agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca
     9901 cttatttaga tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg
     9961 aagttttgac aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca
    10021 aaacaagtga aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa
    10081 atacagaatg gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg
    10141 aatacttgca aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt
    10201 ctatcccagc acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac
    10261 gttatgtagt aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag
    10321 gttcctctgc attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta
    10381 cagcagcaaa agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa
    10441 aatatgtacc agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa
    10501 tgacccatca attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa
    10561 atgtattggc actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact
    10621 tcttcttcca gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag
    10681 cgttaggaga agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa
    10741 aaagctgcct tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta
    10801 acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag
    10861 ggactgtgac acgatttgtg taccgacccc caaaagt
//
LOCUS       DAYYME010000096        10856 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000096 DAYYME010000000
VERSION     DAYYME010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.097"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..84
                     /locus_tag="ACHBFW_002068"
     tRNA            12..84
                     /locus_tag="ACHBFW_002068"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ACHBFW_002069"
     tRNA            88..160
                     /locus_tag="ACHBFW_002069"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ACHBFW_002070"
     tRNA            168..249
                     /locus_tag="ACHBFW_002070"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ACHBFW_002071"
     tRNA            267..339
                     /locus_tag="ACHBFW_002071"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ACHBFW_002072"
     tRNA            351..422
                     /locus_tag="ACHBFW_002072"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ACHBFW_002073"
     tRNA            439..524
                     /locus_tag="ACHBFW_002073"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ACHBFW_002074"
     tRNA            535..608
                     /locus_tag="ACHBFW_002074"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ACHBFW_002075"
     tRNA            618..691
                     /locus_tag="ACHBFW_002075"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ACHBFW_002076"
     tRNA            711..783
                     /locus_tag="ACHBFW_002076"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ACHBFW_002077"
     tRNA            811..884
                     /locus_tag="ACHBFW_002077"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ACHBFW_002078"
     tRNA            898..971
                     /locus_tag="ACHBFW_002078"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ACHBFW_002079"
     tRNA            995..1084
                     /locus_tag="ACHBFW_002079"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ACHBFW_002080"
     tRNA            1097..1170
                     /locus_tag="ACHBFW_002080"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ACHBFW_002081"
     tRNA            1189..1264
                     /locus_tag="ACHBFW_002081"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ACHBFW_002082"
     tRNA            1266..1336
                     /locus_tag="ACHBFW_002082"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ACHBFW_002083"
     tRNA            1350..1423
                     /locus_tag="ACHBFW_002083"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ACHBFW_002084"
     tRNA            1448..1535
                     /locus_tag="ACHBFW_002084"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ACHBFW_002085"
     tRNA            1557..1628
                     /locus_tag="ACHBFW_002085"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ACHBFW_002086"
     tRNA            1651..1740
                     /locus_tag="ACHBFW_002086"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ACHBFW_002087"
     tRNA            1753..1826
                     /locus_tag="ACHBFW_002087"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ACHBFW_002088"
     tRNA            1845..1917
                     /locus_tag="ACHBFW_002088"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ACHBFW_002089"
     tRNA            1921..1993
                     /locus_tag="ACHBFW_002089"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ACHBFW_002090"
     tRNA            1998..2068
                     /locus_tag="ACHBFW_002090"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ACHBFW_002091"
     tRNA            2081..2154
                     /locus_tag="ACHBFW_002091"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ACHBFW_002092"
     tRNA            2166..2237
                     /locus_tag="ACHBFW_002092"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /gene="metK"
                     /locus_tag="ACHBFW_002093"
     CDS             2503..3696
                     /gene="metK"
                     /locus_tag="ACHBFW_002093"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFM9318369.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ACHBFW_002094"
     CDS             3826..5298
                     /locus_tag="ACHBFW_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM9318370.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ACHBFW_002095"
     CDS             5381..6169
                     /locus_tag="ACHBFW_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9318371.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ACHBFW_002096"
     CDS             complement(6236..6904)
                     /locus_tag="ACHBFW_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFM9318372.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ACHBFW_002097"
     CDS             7019..8731
                     /locus_tag="ACHBFW_002097"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9318373.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="ACHBFW_002098"
     CDS             8734..10506
                     /locus_tag="ACHBFW_002098"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9318374.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(10588..>10856)
                     /locus_tag="ACHBFW_002099"
     CDS             complement(10588..>10856)
                     /locus_tag="ACHBFW_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9318375.1"
                     /translation="SKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTE
                     LGIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT         3114 a         1960 c         2503 g         3279 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggcagaaaag gggcaattta aatttctatt tttcctagtt tttctactag
    10621 ttgattttgc atatttttag ttgtatgagt ataaatcttt aatgttgttt ttgaatcttc
    10681 atgtcctaca cgatccataa tggcttttaa tgggattcct aattctgtaa gtagagaaat
    10741 gtgggaatgc cttaacatgt gagaagaaac ttttttatct aggtttaatt ctttggatac
    10801 tgctcttaaa gattgattat atgcttgaat agatatcggg ttaccatttt tagaga
//
LOCUS       DAYYME010000097        10392 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000097 DAYYME010000000
VERSION     DAYYME010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.098"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(130..615)
                     /locus_tag="ACHBFW_002100"
     CDS             complement(130..615)
                     /locus_tag="ACHBFW_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFM9318376.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    705..1050
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1095..1736
                     /locus_tag="ACHBFW_002101"
     CDS             1095..1736
                     /locus_tag="ACHBFW_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9318377.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1755..2390
                     /locus_tag="ACHBFW_002102"
     CDS             1755..2390
                     /locus_tag="ACHBFW_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9318378.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2408..3223
                     /locus_tag="ACHBFW_002103"
     CDS             2408..3223
                     /locus_tag="ACHBFW_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9318379.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3542..4978
                     /locus_tag="ACHBFW_002104"
     CDS             3542..4978
                     /locus_tag="ACHBFW_002104"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9318380.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5167..7599
                     /locus_tag="ACHBFW_002105"
     CDS             5167..7599
                     /locus_tag="ACHBFW_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM9318381.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            7829..8896
                     /gene="rlmN"
                     /locus_tag="ACHBFW_002106"
     CDS             7829..8896
                     /gene="rlmN"
                     /locus_tag="ACHBFW_002106"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM9318382.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            8886..9092
                     /locus_tag="ACHBFW_002107"
     CDS             8886..9092
                     /locus_tag="ACHBFW_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318383.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9089..10192)
                     /locus_tag="ACHBFW_002108"
     CDS             complement(9089..10192)
                     /locus_tag="ACHBFW_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM9318384.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         3428 a         1783 c         2265 g         2916 t
ORIGIN      
        1 actcgtatta gttttcagcg ttcatactag ttgttacatt gtttgattgg ccaaaagaat
       61 ttctgatata ttttgtgcta aataaagaaa aagacgcttg aattgggaaa taagaaaact
      121 actctatcat tacaaatttt cataaactgg cagatgttct aagtaaacaa gtatcttttc
      181 aaagtcggaa aaatccataa tcaacgtagt ggtattgata tttggatgaa agcctacggt
      241 atcttgacga tcaatagcag catcgatcac gacttggatt tttttctctt tgtcgtgcat
      301 caatgctaat ggagtcactg tacctgctgg aacgcctagg aggtcaggaa gctgttcttc
      361 agaaacaaaa gataagcgct tttcttttaa ttgttcagct agttgtttta aatcggcgtg
      421 cttttcatca ggcaagatga caagatagat ttgttttcct tttcttgatt tcaataatag
      481 attttttact tgcggaccag gtagggaaaa agggacattt ttgactgacg taatcgcagg
      541 atgatcgact ttttgaaaag agatacccag tttttttagt agttcataag cttcttgttc
      601 ggttgcacga ctcatcgcgc atcaccctcc tttttttatt agtgtatcat aaaaaagagt
      661 tgacaaatga aaaataatca tgtaaattac acgtaaagtt aaaacaaatg acatcgaaaa
      721 gaagagtagc cgttaggtgt ttttagagag tctctggttg gtggaaagag gcagcagtta
      781 accgtgaaac acatcttgga gtcgattttc tgaagcgtat agtaggaaaa tccggggagc
      841 ccgttacagc tgcggtcgtt gctagatgac cagataaggc tggcttttcc gtgaggagtc
      901 ggtaaaaaaa ggtggaacca cgtgattaac gtcctttgat cttcggatca aaggactttt
      961 tgttttcatc tgaacggcaa cgactgtgtg aggttttttc tgcaaggaaa aaataaacag
     1021 aggtggaacc acgtataata cgtcctcttg gcatttgcca agaggatttt tttattttaa
     1081 aggaggaaaa gaagatggag tatttgattg aagttatgcc gcaattatta gtaggagcac
     1141 tcacaacatt gaaattattt ttatttacct tgattggctc attaccatta gggattgttt
     1201 tagcattagg attgtctttg cattttaagc cgctacgcta cttgcttcaa acatatgtat
     1261 gggttatgcg gggaacaccg ctgttgttgc aactgatttt tattttttat gggttgccga
     1321 cgattgggat cgtttttgat cgtttcgaag cagcactcat tgcctttatc ttaaactatg
     1381 cagcttattt tgcagagatc tttcgtggag ggatacaatc gattcctcaa ggtcaatatg
     1441 aagcagcgca agttttgcaa ttaagtccct ttcaaacggt gacgaaaatc gtcatcccac
     1501 aagtgacaaa gattgtttta ccttctgtag ggaatgaagt tatcaacttg gtcaaagatt
     1561 cttccttgat ctatatatta ggtttaggtg acgtaatgcg tgctgggaaa atcgcgatgg
     1621 aacgcgacgc gactttactg cctttgctag gcgtggcaat catctatctt acattgactg
     1681 gtattctaac aatcgtaatg aaacaagttg aaggaaaatt aaattattac cgctaactga
     1741 taggaggaag aaatatgatt gaattgaaga aagtgaacaa agcatttggt gaaaaaaaaa
     1801 taatcaaaga tttgaatcta atcattccag atgggaaaac attagctatc gtaggtcctt
     1861 ccggtggcgg taaaaccacg cttttacgta tcttggctgg cttagaaagt tctgacagcg
     1921 gagaatttta tttagacggt gtaccgttca acccattcga gcttaaaatg aaagaacaag
     1981 ttgtaggagt cgtattccaa gacttccagc tatttccaca tctgaatatt tttgaaaaca
     2041 ttaccttagc accaaaaatg gtattgaaac agacaaaaga agaatacacc aaaaaagcag
     2101 aagaattagc ggcttcatta gggattagtg atttattgaa tatctatcct tatcagcttt
     2161 ctggcggaca aaaacagcgg gtagcgattg ccagagcatt ggctatgaat ccacgggtat
     2221 tggcttatga tgaaccaaca agtgctttag atcctgaatt acgccagcaa gtcgagcgtt
     2281 tgatcctatc attgaaagaa gatggggtaa cccaaattat tgtcacccat gatttagtat
     2341 ttgcagaaaa tgtcggtgat caattactaa aagtttcccc aattcattaa gaaaggagaa
     2401 gatagagatg aaaaaaacca gttttctatt cgctttgatc gcaggtcttc ttctttttgc
     2461 aggctgttcg aacaaaaaaa cttcagcaga taattgggcg actatcaacg aagagaaaaa
     2521 aatcgttata ggtttagacg atacatttgt tcctatggga tttagagata aagacgggaa
     2581 gctcacagga tttgatattg atttagccaa agcagtcttt gaacaatatg ggattacggt
     2641 agactttcag cccattgact ggtcaatgaa ggaatttgaa ctgaacaatg gaacgatcga
     2701 tttgatatgg aacggctatt ccaaaacgcc ggcaagagaa aagaaagtgc aatttacaaa
     2761 accttatatg gaaaacgatc aagtgctgat cacacctaaa gaatcaggaa tcacaaactt
     2821 tggacaaatg aataataaga gattaggtgc gcaaaatggg tcatctggtt atgatgtatt
     2881 tacgaaacaa ccagaagtat taaaagagat agtcaaaaac caagatgctg ttttgtatga
     2941 tagttttaat gaagcattga tcgatctgaa aagcagacgg gtcgatggtc tgctgatgga
     3001 taaagtatac gcagactatt atttgaaaca aagaaacgaa ctagaagact tcaacataac
     3061 aaaaggttct tatgagagtg aggattttgc agttggtgca cgcaagtcag atagtacatt
     3121 agtgaaaaaa atcaatgaag ccctagatca actagagaaa acaggtgaat tccaaaaaat
     3181 atccgataaa tggtttggcg aagatgttac gccgagaaat taacaaatag gggactgtga
     3241 caaaagacgt gtcacagtcc ctttttccga ataaacagtg ttctcctgcc aactccttgt
     3301 gactcggagc tgaatggcag gctcacaacc tcttggtaaa cggtgttcaa aagccagttt
     3361 ttcggtatta agccaaattt atgcaaaaat gtagaaagcc attttcacaa aatttttctt
     3421 actactcgaa actaagcgac tttttcacaa cctcctttac ataattttat tttgttgtgg
     3481 aaggggtttc gtgttattct aaaagaggaa cgattgaagg ttataggaaa ggaagagatg
     3541 aatggaacac aaagaactta cctcatttcc aaaagatttt ctgtggggct ctgcttctgc
     3601 agcgtatcaa gtagaaggag cttggcaaga agatgggaaa ggagaatcag tctgggatcg
     3661 atttgtacgg atacctggaa agacatttaa aggaacgaac ggtgatttgg cagtagacca
     3721 ctatcatcgt tataaagaag atattgcttt aatgaaagaa caaggtctga aagcttaccg
     3781 cttctctgta gcttggacac ggatttttcc taatggacga ggagaagtca atcaagctgg
     3841 gctcgctttt tatgagagat tgatcgacga attgattgaa aacgatattg aacctatgtt
     3901 gactttatat cattgggatc ttccacaagc attgcaagat gaatataatg gttgggaatc
     3961 acgtcagatc attgaagatt tcactcatta tgcagaaaca ttatttgagg cattccgcgg
     4021 gaaagtccat tactggatta gtttgaatga acaaaatatc ttcacttcat taggttattt
     4081 gttagcagca catccgccag gtgtgacaga cccgaaacga atgtatgaag ttaaccatat
     4141 tgctaattta gcaaatgcat ctgtcatcaa taaatttcac gagttagaaa tgcctggaaa
     4201 aatcggacca agctttgcgt atacgccaaa ttatccaatt gacagtaatc ctgaaaatgt
     4261 tctggctgct gaaaatgcag aagacctgat ggccaattat tggatggatg tctatatgtg
     4321 gggaaaatat ccgattgctg ccatgaggtt cttagaagaa aaaggatggg caccaacgat
     4381 tgaagctggc gatgcagaat tgctagaatc tgctaaacca gattttcttg ggatcaatta
     4441 ctatcaaacg gcgacgaatg cattcaatcc attaaacgga gttggtgctg gaaaaatgaa
     4501 tacgacagga aaaaaaagtt catcagaaga aactggagtt cctggaatgt ataaaaaagt
     4561 tgagaatcca tttgtcgaac gaacaaattg ggactgggag attgacccag aaggattaag
     4621 gatcggactg cgtcgtatca caagccgtta ccgcatgccg gtactgatta cggaaaatgg
     4681 cttaggagag tatgacaagt tgacagaaga taaacaaatc catgacgact atcgaatcaa
     4741 ttacttacaa agccacgtca aagccatcaa agaagcaatt tcggatggag cagaagtcct
     4801 aggctattgc acctggtctt atacagattt gcttagctgg ctgaacggtt atcaaaagcg
     4861 atatggattt gtatatgttg atcaagacga aacacaagaa ggatctttgg aacgatataa
     4921 gaaagacagt tattactggt accaaaaact gattgaagaa aatggagaga acctttagtt
     4981 cacatactta aaacaagaag gtacggctgg atatggccgt gccttttctt gataaaatca
     5041 tttagaaatt aagaaaaact gtaaaataag ctgaaaaatg aaagaaagcg tcagttcttt
     5101 caaacaaaac tttagttccc ttactttttt ttgtataatg aaacttgatg aaatgaggga
     5161 taataaatgg cacaaaagaa acggcctact aaaaaaagga aaaagacgaa aaaaacgaaa
     5221 aagcaaaaac agcagcagga acaaatgctg acgattggtg ttgggtttgc cttgatttta
     5281 tttgctgtct tcggcttttt aaaattaggt tttttaggaa tattgattgc aaacggcttt
     5341 cggatcattg caggaaatac ttaccaaatc ctctgtcttt tattagccgt attaggattt
     5401 tggattgtca ttaagaatac agagttttca attggtaaaa accgacgctg gtttggcggc
     5461 atactctttt atttcggtat cttattattg ttacatgccc atttatttgg caaactccat
     5521 actggggaac caaatatcat ggggacgaca tgggatttgt tagccagtga tataaaacaa
     5581 agtcaagtag acaataacgt tggtggtggg atgattggag caatccttta tcattttact
     5641 tacttcttga ttgcacagcc tggaagttat ttagcagcag tgctattact ggcaggagga
     5701 gcattcctgt tcagtaatct ggaaggctat cagctgttaa atggtatcca atcaattgga
     5761 gaacgagtac aggaattatt ggaaggtgat cctgccaagc aagctcgaaa gcaagcggca
     5821 aaggaagaga gaatgaaaca gcgcgcagaa gcgaaagaag caaggcgctt agctgcaaaa
     5881 gaagctgcag aatatgagaa gaaaaaagcg gtgagccagc aaagggaaaa tactgctgat
     5941 gaatggcagg aaccagaaaa tacagagccg gagcagctaa gttttgttcc aatcgacagt
     6001 tttcaagaaa atatccatcc tgcgaatcta gagaaaccag taccagatac accaaaacag
     6061 accaatacag cagaaggttt ttcagatgag ttgccagagg atgacggtac ctcattagaa
     6121 tttgagattg aagcagaaca ggaaaatcaa gactatgaat taccgacagt tgatttgctt
     6181 gattctatac caactgtgga tcaaagtgat gagtataaaa aaattgaaaa aaatatcggc
     6241 gtattagaac aaactttcca aagtttcggt gtcgatgcca aggtcgtcaa agccagctta
     6301 ggaccatccg tcacaaaatt cgaagtccag ccggctgtag gggtaaaagt aagtaaaatt
     6361 gtgaacttaa cagatgatat cgcgcttgct ttagcggcga aagatgtccg gatggaagca
     6421 ccgattccag gtaaatcact gattggtatt gaagtaccaa atggaaagat cagtatggtt
     6481 tcctttagag aaatcatcga agcacaacca aaccacccag ataaattact agaagtacct
     6541 ttaggacgtg atgtttctgg acgggtacaa acggctgatc tttcgaagat gcctcactta
     6601 ctcgtggctg gttcgactgg tagtgggaag tcagtagcaa ttaatgggat tatcacaagt
     6661 atattaatgc gtgctaagcc ccatgaagta aaactgatga tgatcgatcc gaaaatggtt
     6721 gaattgaata tgtacaacgg catacctcat ctgttgacgc cggtcgtgac gaaccctaga
     6781 aaagcagcgc aagccttaca aaaagtagta caagaaatgg aagaacgata tgaaaaattc
     6841 gcggcaaccg gagtaagaaa tatttctggt tacaatgaat ttgttcaaca gaaaaacttg
     6901 gaaaacggaa cgaaacatcc aaccttacca ttcatcgtag tgatcgtaga tgaattagct
     6961 gatttgatga tggtagccag caatgaagtt gaagatgcga ttattcgttt agcgcaaatg
     7021 gctcgggcag cggggattca tatgatccta gctactcaac gtccaagtgt ggatgttatc
     7081 acaggaatca tcaaagcaaa tgtaccttcc agaatggcat ttgcggtatc aagcggtaca
     7141 gattctcgga cgatcattga ttctaatggg gcagaaaagc tcttagggcg aggagatatg
     7201 ctattcttgc caatgggtga gaataaaccg atccgtgtgc aaggagcatt catttctgac
     7261 cacgaagtag aacgggtcgt tcaatttgtt acagatcagc aagaagctca ttatgaagag
     7321 aaaatgatgc cgacagatga agttgaaacg acaggtgctc cagaacagcc acaagatgag
     7381 ctgtttgaag aagcaaaagc acttgtcgtt gaaatgcaaa cggcaagtat ttcccttctc
     7441 caaagaagat ttcggatcgg ttacaaccga gcagcacgtt tagtagatga actggaagct
     7501 catggagttg ttggcccgtc agaaggaagc aagcctcgga aagtattcat tgagcaggaa
     7561 gaaacatcag aaacgttgcc ggacgataat gacatgtaaa acttgtgtga tgaagggttg
     7621 aaaaacgaat aaccaataaa aagacaaggc atgatgatac caaaagaaaa aatggttttt
     7681 ttccatcttt tctaatacgg tatgatgtct tgtcttttta gtttttgtga gattaaagtt
     7741 ctattcgatt tatttgtgaa gtattgcatt aaggaaatga atcatgtata gtatgtagcg
     7801 aaactttgag aaaaagaggg aaaatacttt ggaaaaacag tccatttatg gcttgacgaa
     7861 tgaagaatta atcaattggt ttatagaaaa cggcgagaaa aaattccgag ctgcccaagt
     7921 ttgggaatgg ttgtatcaaa aacgtgtaag taatttcaca gaaatgacga atctatcgaa
     7981 acaattgatc gaaaaattat cggagcattt tatcatcaat cctctaaaac agatggtcgt
     8041 ccaagaagct tcggatggta cagtaaaata tttgtttgaa ctccctgata aaaacatgat
     8101 tgagactgta ctgatgagac aagaatatgg cttgtcagtt tgtgtgacga cacaagtagg
     8161 atgtaatatc ggctgtacgt tttgtgcaag cgggctgctc aaaaagaacc gtgatctgac
     8221 tgctggagaa attgttgcac agatcatgat ggtccaacat tattttgatg agcgcaatct
     8281 tggcgaacgc gtttcacatg tcgttgtaat ggggatcggt gagccatttg ataattacga
     8341 caatgtaatg gactttttgc atatcatcaa tgatgccaaa ggattggcaa ttggtgctcg
     8401 gcacattaca gtttccacta gtggattagc acacaaaatc aaggaattcg ctaataacgg
     8461 cttgcaagtc aatctggcta tttccttgca cgcgccgaat aatgaggtgc gaacatcgat
     8521 catgcggatc aaccgaagtt tcccaattga gaaattgatg gaagctgtgg atgagtattt
     8581 agagaaaaca aaccgtcgaa tcacgtttga atatatcatg ttgaatcaag taaatgatag
     8641 accagagcat gcgcaacaat tagcagatct attaaaagat aagaaaaaat tagcatatgt
     8701 caacttgatt ccatataatc cagtcagtga acatgatcaa tattctcgca gtccaaaagc
     8761 tgatgtattg agattttatg atgtattgaa gaaaaacggc gtaaactgtg tgatacgtaa
     8821 agaacatggt acagatattg atgcagcttg cggacaattg cgaagcaagc aaatgaaaaa
     8881 aacagttgcc aaataatcgt atcctactcg ttttttcaat taccataaaa ggagcagttt
     8941 tcttaattaa ggaactgttc aacttaaata agagaaaaga ttctatagcg aaagctataa
     9001 agcttttttt gacaagaaaa accctcatca agcaaagaaa tgtcctcaat ttgatgacag
     9061 cctgcttgat gggggtttta gcaattattt aactgatttt cttatatcct tgctggcttt
     9121 cttgtgtagc atgataagtt tctacgtcaa gaatggcctg tttaaatttt tctcgggtga
     9181 tttcataagg cacatgttta atatcatttg ttgccatggc tttatctaag attgcttcta
     9241 aatcggtcgt gacattgaga ttcatgtcgg ttaacgatac tggaagttgg ttctttttca
     9301 taaacacata gattttatca aacaaatcga actgctgatc catcatcaat aaggtcagta
     9361 caccataact gacagatgct ccgtgaaggt atccctccat tttttctaga acagtcgtac
     9421 cgtaatagaa tgagtgtgcc atagcagaat tatagttatt gttaactaaa acagaaacca
     9481 atccggtagt aacaatgata tccgaaacga cttgttcaat tgcatatgag ggacgatcgt
     9541 cggcactatc tttcaatgct tgcgtaccat aagtcaagag acgatcaatg cacatttttc
     9601 cgatttggat tcctaatcta tccgtgtagc ctagatcatc gccacgagca gaaaaagtag
     9661 attcgacttc tttggataaa gcatcgccaa tacctgccca taagtattta tcaggtgctt
     9721 cggcaataat tgctgtatca atgaaggtgt gtacaggtgg ttttgaagga aagaagagtc
     9781 cgtccattac gccttcttcg ttatagatga cacataccgc agtgacgggc gaacagttcg
     9841 aaccaatagt gggaaaggtg aagactggtt tagctagttt ttcaccaatg acttttacag
     9901 tatcgcaagc acggccacca ccgactgcaa acaggacgtc tgctttttgg acactttcat
     9961 gctggatcaa ctgttcaata tttccataag ttgaattacc cccgtaccag agactggcga
    10021 caacctctat ttcctgtgtc aaagctactt ttattttgtt ttctgccttt gaaagtgcag
    10081 tcttccctcc aatcagcact acttttgtcc catattcttt gacgattcca tttgcttttt
    10141 catacgcatc tgttccaatg gtataatttg gtaagaaaac actttgactc atattcattt
    10201 ccttctttct tcgttgaaac gggttaaaat tttaaagagt gatcgatctg actgcggctg
    10261 tagatggagt agaggtgaac ctaagacagg ataagtgatg aataaggtgg catgaacacc
    10321 ttgaaatcat caataagtat cctaaaaacg aacaagtaca aaaacattga aaacgacaat
    10381 aatgaatttt tg
//
LOCUS       DAYYME010000098        10076 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000098 DAYYME010000000
VERSION     DAYYME010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.099"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..579)
                     /locus_tag="ACHBFW_002109"
     CDS             complement(<1..579)
                     /locus_tag="ACHBFW_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318385.1"
                     /translation="MSQWNTIDELRDIFIRKYTDSELSNELEKACSEEYELMRDYNGR
                     QILELLQNVDDAYGDIKSENDASNDEVKVQITYKNNILEVGNTGTSFSKDTIERLCLG
                     RASNKSSQNIGNKGTGFRSLLNDAEWVELYSGEFAIRFSEEFTKKLFEQYVSRDSDKF
                     SELIFEQKKNWKKEDYDLCFPIMNCPQPINKID"
     gene            complement(702..2252)
                     /locus_tag="ACHBFW_002110"
     CDS             complement(702..2252)
                     /locus_tag="ACHBFW_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9318386.1"
                     /translation="MAGKQVTVIPMKPRQWAAENTEEKPKLKVAAYCRVSTEMEEQAS
                     SYEAQVQHYTDYIQRNPDWELVGIFADEGISGTGTKKRDGFNRMIEACQNGEVEYIIT
                     KSISRFARNTVDCLQYIRQLKELKIAVFFEKENINTMDAKGEVLLTIMASLAQQESQS
                     LSQNTKMGVQYRFQQGQLRINHNHFLGYTKDEDGNLVIEPKEAEVIKRIFREYLEGSS
                     LQEIANGLMADGILTGGKRKLWRGEGVRLILRNEKYMGDALLQKTYTTDFLTKKRVKN
                     DGSYAQQYYVEDSHPAIIPKDIFMQAQQELDRRKSMKNKKSQCFSGKYALSGITVCGD
                     CGNAYRRVHWKNRGTVWRCKSRVDKREHNCTGRTIYETDLHGAVIKAINETLIDRDAF
                     LQQLNDNINSVMTDGLTDRLAELDSQLKELEAEIISLAVGGQGYDELASQIFSLRDER
                     DAVAKQIAANTNLQQRVDEMVVFVKEHDVINEYNEVLVRRLIEKVTIFEKNIVVDFKS
                     GVRVTVEI"
     gene            complement(2254..2619)
                     /locus_tag="ACHBFW_002111"
     CDS             complement(2254..2619)
                     /locus_tag="ACHBFW_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9318387.1"
                     /translation="MDEQQAQQIREFFNIYLEGVGLQEAGKRSGINKPHPFMKRILMN
                     EVYLGNEIYPKIIDEDIFFQADLEQERRIKFFKKRKEIKVPDTKVIPTHFKSGKITEK
                     YSDPFKQAQYAYSKIKEVK"
     gene            complement(2667..4220)
                     /locus_tag="ACHBFW_002112"
     CDS             complement(2667..4220)
                     /locus_tag="ACHBFW_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9318388.1"
                     /translation="MKTIHKLARPELPEASKLKVAAYARVSTSSNEQLASLQTQITHY
                     ENHIKNHEQWDYVGVYYDEGISGTKTVKRDGLKRLIKDAELGKIDLILTKSISRFSRN
                     TVDCLNLVRQLTDIGVTIFFEKENINTGDMESELLLSILSSLAESESYSHSENLKWAI
                     RNRMSKGKFKAYPPYGYKRKGYDFHIVAAEAKVVKQMFQWALDGISTGMIAKRLNDGK
                     QFTKRGANWQATSVRSILKNEVYTGTLTMQQTFVDDQYKRRINKGELPMYQIKNNHEP
                     IVSKEDYDRVQELIRRKAEAKGNTGDGKKYTKRYAFSGNIICDKCGSKFKRVHTYWKG
                     NTKKVQWKCTGHLKNKDSCDALPITDEDLKVAYLTMLNKLIFGHKLVLEPFMKSLNKA
                     GANQEGLEVLADEIDKIQEKLEVLASLTASSILSTKVSLEEQSKLQQERAVAQEKQRL
                     IIESVNGKSTQQIELEALYKFARMAEMLTDWDEDLFKKFTNSITVYNRQEVGFELKCG
                     LLLKERLPE"
     gene            complement(4281..4454)
                     /locus_tag="ACHBFW_002113"
     CDS             complement(4281..4454)
                     /locus_tag="ACHBFW_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFM9318389.1"
                     /translation="MTNKKTEINYSDEAAYYISQQILLNLLVTGKINLEEYVKIDNKN
                     RETFNPFLSRIMV"
     gene            4749..5054
                     /locus_tag="ACHBFW_002114"
     CDS             4749..5054
                     /locus_tag="ACHBFW_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318390.1"
                     /translation="MSSFDMEGLEKEYEHFCKSATTKELEEFDESFKQEFEKCFKTIE
                     AEEALYQFLQANINAMNNRMESIESPLFCINYKEQVFQLRNHLILMQNAVNTYLKEN"
     gene            complement(5115..6152)
                     /locus_tag="ACHBFW_002115"
     CDS             complement(5115..6152)
                     /locus_tag="ACHBFW_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFM9318391.1"
                     /translation="MVTFKDLLPEPTESQQAFILDHGKTEDDGQLKYADDAKSYGWNM
                     RQYGKLKAGAVVLNRHPGKITKDRKWEIYGGGYVESISEPDEAGNVTAVITHAFTIEP
                     AIKQGDSFIENFDWNTPSKKKRKKPNSWAYFWDQYGMNEITYADFVALIENRHLIPID
                     DTQSLPSELDLTNEEVEEIEQESSKGFTVLVDEVGPNRTNGTQKRKFTGRHTDWERVN
                     KAKQKTGALGEEIVLDFLIQEAEKNKTKLPEHVSKTEGDGHGYDICAFDQSGNEIHIE
                     VKASKTNFSDGFEMSTNEVAASLGDTPYKIYFVHDLDVASKVCKIKIYDGPITEENFT
                     MVPTSYKIFNK"
     gene            complement(6268..7176)
                     /locus_tag="ACHBFW_002116"
     CDS             complement(6268..7176)
                     /locus_tag="ACHBFW_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM9318392.1"
                     /translation="MGISNLATKYGFQSFSHYSNGRSGAKVDKIVIHHMATTNYDIVP
                     DIWKTREASAHYGIGKNGEIRAYVDEGNTAWHAGNWNANISSIGIEHCNSTGSPNWGI
                     NQATIDASAKLCADIAKRYGLGKLVVNKNIFPHSHFSATSCPGQLLGKLQEIADKANA
                     INNGSSTPSTPASGLYRVRKSWADAKSQIGAYKDLNNAKRAADSHSGYKVFDQNGNQV
                     YPSGSSTSNVKTLVVQVSGLNVRNKPSLSGAVVGSYNKGATWTLPKSEKMTIADGWVW
                     ARCPIGYCAVGKNTGKTESDDYILIS"
     gene            complement(7166..7585)
                     /locus_tag="ACHBFW_002117"
     CDS             complement(7166..7585)
                     /locus_tag="ACHBFW_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin family protein"
                     /protein_id="HFM9318393.1"
                     /translation="MKEVWNWIQLAIAGGGGVIGWYVGGIDGLIYTLVTFVVVDYVTG
                     LLRAINEKKLSSKIGAKGITKKIMIFLLVGVAHMLDVEVIKGGNVLRDAVIFFYISNE
                     GISLLENAVAIGLPVPEKLREILQQLHEKKEETENGN"
     gene            complement(7586..7993)
                     /locus_tag="ACHBFW_002118"
     CDS             complement(7586..7993)
                     /locus_tag="ACHBFW_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HFM9318394.1"
                     /translation="MLKIRNEDLVDVMSFLKGFDLPPKISRVRTKLAKLIQVKVDELY
                     QDEVDLLQKYGKKDEHGNLVQHDGAFSLIPETATEYHKEKADLLNEETHINVDELQDK
                     LPLLVAGFENSEVTISGKDAEMLDLILEQLEDL"
     gene            complement(7980..8147)
                     /locus_tag="ACHBFW_002119"
     CDS             complement(7980..8147)
                     /locus_tag="ACHBFW_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318395.1"
                     /translation="MNEEKEEVIVTEPIEADIPKPMKVGTDMVDLLVMVVDDLCVQIG
                     DLKEKIDNAQN"
     gene            complement(8157..9626)
                     /locus_tag="ACHBFW_002120"
     CDS             complement(8157..9626)
                     /locus_tag="ACHBFW_002120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318396.1"
                     /translation="MSITSGVTTNPYSNSGDYLVIYKLPLEGKVAPSVISWNGRKDTS
                     DNIVLLYTGGAWEQISTVTSSSNATYSWTLTAEQISGITTDSVYLAFFSVKTGTYAGI
                     YNSSNTPFTLNPSDGSSYHQISYTGSSGAITVPSGTLKMRVRVNTSIKPDEYEGNIYP
                     TTTRQDYTNANTLYSAILMQKDLINLRVGKGDVINQINISPESILIAGNKVHITGQTT
                     IDSAVISSAMIQNAAITNAKIADATIQSAKIASIDAAKITTGTLSAARIGANSITADK
                     IAANILTALTASSSIRITGTTIGYYSGSTLVTEINSQGMTIRRGGTTVGTIGANNISG
                     HSDWRGLVFDLEYGTEYMSWAHKDSSSASVYTQKLSWYARTLQSGMEKGFHFDDDIYL
                     PSMLRIGTQHQISVGGITFSSSLYTKIGSSSGSAGIAFNGSNLMLGDDGKWVDFGVIR
                     EICAKLAGRTIALPTGFSSNGTASGWYNPQSFNSMTTYS"
BASE COUNT         2716 a         2312 c         1758 g         3290 t
ORIGIN      
        1 atcaattttg tttattggtt gaggacagtt cattattgga aaacataaat cataatcttc
       61 ttttttccaa tttttcttct gctcaaaaat taattcactg aatttgtcag aatctcttga
      121 tacatactgc tcaaaaagct tttttgtgaa ttcttcacta aatcgaatag caaattctcc
      181 cgaatacagt tcgacccact cagcatcgtt tagcaaggaa cgaaaaccag tccccttatt
      241 tccgatattt tgtgatgatt tattactcgc tcttcctaag cacaatcttt caattgtatc
      301 ttttgaaaat gaagtgcctg tattcccaac ttccaagata ttatttttat atgttatctg
      361 aactttcact tcgtcattgc tagcatcatt ttctgatttt atatctccat aggcatcgtc
      421 tacattctgt aataactcta aaatctgacg cccattataa tcacgcatca attcatattc
      481 ttcgctgcag gccttttcaa gttcattact caactcagaa tccgtatatt tacgaataaa
      541 aatatccctt aattcatcaa tggtattcca ttggctcaca gaacttcgcc cccagcctat
      601 tttttacata atatattata tcatcaatca ctttatgaaa tccaatcaat aaagacgtta
      661 atatggctac ccacattaac gtctagtcct atctattcaa attaaatttc tacagtcact
      721 ctcaccccac ttttgaaatc aacaacaatg ttcttttcaa aaatggtgac cttctcaatc
      781 aaccttcgaa caagcacttc attatactca ttgatgacat cgtgttcttt tacaaagact
      841 accatctcat caacccgttg ttgcaaatta gtattcgctg ctatttgttt tgcaaccgca
      901 tctcgttcat ctcttagtga aaatatttgg cttgccaatt catcgtaacc ctgaccgcca
      961 actgccaggc taataatttc agcttcaagc tctttcaact gactgtctag ttctgccaat
     1021 cggtcagtta atccatcagt cataactgag ttgatattgt catttaattg ctgaagaaaa
     1081 gcatcacgat cgattaacgt ttcattgatt gccttgataa cagctccatg caggtctgtt
     1141 tcgtagattg ttcgacccgt gcaattatgt tctcgtttat ccactctgga tttgcatcgc
     1201 cacacagttc ctcgattctt ccaatgaact ctgcggtaag cattgccaca gtcaccgcaa
     1261 actgtgatac cagataacgc atacttccca gaaaagcact gtgacttttt gtttttcatt
     1321 gatttacgcc ggtctagttc ttgctgggct tgcatgaaga tatcttttgg gataatggcc
     1381 ggatggctat cttcaacata atactgctga gcgtaactcc cgtcattctt cactcgtttc
     1441 tttgtcaaga aatcggtagt atatgttttt tgaagcagcg catcccccat gtacttctcg
     1501 tttcgaagaa tcaatctgac tccttctcct cgccagagct ttcgcttccc tcccgttagg
     1561 atgccatcag ccattagacc attagcaatt tcttgcaagg aactgccctc aaggtattcg
     1621 cggaagatgc gtttgatgac ttctgcttcc tttggttcaa tcaccaagtt accatcttcg
     1681 tccttagtgt acccaagaaa atgattgtgg ttgatacgta gctgtccctg ctggaaacga
     1741 tactgtacgc ccatcttggt gttttgactt agtgactggc tttcttgctg ggccagactt
     1801 gccataatgg tcaaaagtac ctcacccttc gcatccattg tattgatgtt ttccttttca
     1861 aaaaacactg caatcttcag ttcttttaac tgtcgtatgt attgcaagca atcaaccgta
     1921 ttacgggcaa agcgtgaaat tgattttgtg atgatgtact caacttcgcc gttttgacac
     1981 gcctcaatca tccggttaaa accgtcacgc tttttcgtcc cagttcctga aatcccttca
     2041 tccgcaaata ttcctacaag ctcccaatcg ggatttcttt gaatgtagtc cgtatagtgt
     2101 tgaacctgtg cttcgtaaga actcgcctgt tcctccattt cagtagaaac acgacaatag
     2161 gctgccactt tcaactttgg tttctcttcg gtattctctg ctgcccattg tctaggcttc
     2221 attggaataa ccgtcacctg ttttcctgcc atttcactta acctccttga tttttgaata
     2281 tgcatattgc gcttgtttga atgggtcaga atacttttcg gtaatcttgc cagatttaaa
     2341 atgagtgggt attaccttcg tatctggcac tttgatctcc ttgcgttttt taaagaactt
     2401 aattcttcgt tcttgctcca gatccgcttg gaagaaaata tcctcatcaa tgatttttgg
     2461 atatatctca tttcctaagt aaacctcatt catcaaaata cgtttcataa agggatgcgg
     2521 cttgttaatg ccagagcgtt tgccagcttc ttgtaagcca acaccctcaa ggtaaatatt
     2581 gaagaactca cgtatttgtt gagcttgctg ttcatccacg ttgatttctc cattcttaat
     2641 gacatacccg tatggaattg tattgttcat tctggtaacc tctctttcaa aagtagtccg
     2701 cacttaagtt caaatccgac ttcttggcgg ttgtaaactg taatgctgtt tgtgaacttc
     2761 ttgaataaat cttcatccca gtccgtcaac atctctgcca tccgcgcgaa tttgtataag
     2821 gcttctagtt caatttgctg agtgcttttg ccgttgactg actcaatgat gaggcgctgc
     2881 ttttcttgtg cgacagccct ctcttgttga agtttgcttt gttcttctaa agacaccttc
     2941 gttgataaaa tactggaagc agtcaaactt gccagaacct caagcttttc ttgaatcttg
     3001 tcgatttcat cagcaagcac ttcgagccct tcctgattcg cacctgcttt attcaaagac
     3061 ttcataaaag gttccagaac caacttgtgt ccgaaaatca acttattcaa catggtcaaa
     3121 taagcaacct tcaaatcttc atccgtaatt ggtaatgcat cgcagctatc tttattttta
     3181 agatgccccg tacacttcca ctgaaccttc ttcgtattac ctttccaata agtgtgaacc
     3241 cgcttgaatt tgctgccgca tttatcacag atgatgttgc ctgaaaatgc gtatcgcttg
     3301 gtgtactttt tcccgtcacc agtattccct ttggcctcag cttttctccg aatgagttct
     3361 tgaactcggt cgtaatcttc tttagatact atgggttcgt gattgttttt gatctggtac
     3421 attggcaatt cgcccttgtt tatgcgtcgt ttgtactggt catcaacaaa ggtttgttgc
     3481 atcgtcaaag tcccggtata aacttcattt ttcaaaatac ttctgactga tgttgcttgc
     3541 caatttgccc cacgtttagt gaattgtttt ccatcgttta accgcttggc aatcattcca
     3601 gtcgaaatgc catctaatgc ccactggaac atttgtttaa caacttttgc ttcagctgca
     3661 acgatgtgaa aatcatagcc tttccgcttg tagccatacg gcgggtaagc tttgaatttc
     3721 cctttagaca tccggtttcg aatcgcccac ttcaagtttt ctgaatggga ataggattca
     3781 ctttctgcca agcttgataa aattgaaagc aacaactcgc tttccatatc accagtatta
     3841 atgttttcct tttcaaaaaa gatggtgaca ccaatatcag ttagctgccg aaccagattc
     3901 aagcagtcaa ctgtatttct tgaaaatctg gaaatagact ttgtcagaat caaatcaatc
     3961 tttccaagct ctgcatcctt aatcaatcgt tttaaaccat cacgttttac agtcttggtt
     4021 ccagaaatcc cttcatcata ataaacaccg acatagtccc attgttcatg gttcttgatg
     4081 tgattttcat agtgagtaat ctgggtttgt aaacttgcca gctgttcatt acttgatgtt
     4141 gatacacgag cgtatgctgc cacctttagt ttggaagctt ccggcaactc tggacgtgcc
     4201 aatttatgta tcgttttcat cagctcaact cctttccatt aggtgacatg ttagctctaa
     4261 agtgccgtta tatcaacgtt tcagaccatt atccttgata aaaaaggatt gaatgtttcc
     4321 cggtttttat tgtcgatttt gacgtattct tccaggttga ttttaccggt aacaagtaag
     4381 ttcagaagga tctgctgtga aatgtaatag gctgcttcat ctgaatagtt aatttctgtt
     4441 ttcttgttag tcattgcttt cgcctcctca agatacaggc gaagaaaatc catgaaagta
     4501 aacccaatca caaattttcc tttgcctttc atccatatgc gaaagaaata accgactcga
     4561 acccacaaaa cgaaaaaagc ctgcaagaat gaaacgaatc actcttgcag gcttctaagt
     4621 ttgtttatat atagtaggaa ttattctttg taaattttga ctttttactt cccaaacatc
     4681 aaataagcct ttacaagctt catccaataa ataacaatat aaatatcaat tgtgggaagg
     4741 agaaagttat gagtagtttt gatatggaag ggttagaaaa agaatatgaa catttttgca
     4801 aatctgcgac aacaaaggaa ctggaggaat ttgatgaaag cttcaaacaa gaatttgaaa
     4861 agtgctttaa aaccatagag gctgaagaag ctttatacca gtttttacaa gccaatataa
     4921 acgcgatgaa taatcgtatg gaatcaatag aatcgcctct tttctgcatc aattacaaag
     4981 agcaggtatt tcagttaaga aatcatttaa tcctaatgca aaatgctgta aacacctacc
     5041 ttaaagaaaa ctagacgaaa aaaaggcccc acaataaaac ttgtgaggct gttttgaatt
     5101 ataaagacgt caaactactt attgaaaatt ttgtagctcg ttggaaccat cgtgaagttt
     5161 tcttcagtaa ttgggccatc ataaattttg attttgcaaa cttttgacgc cacatcaagg
     5221 tcgtgaacaa agtaaatttt atatggtgta tctccaaggg aggcagcaac ctcattagta
     5281 ctcatttcaa atccgtcaga gaagtttgtt tttgaagctt tcacttcaat atggatttca
     5341 ttacctgatt ggtcaaacgc acaaatatca tagccatgac catctccttc agttttagaa
     5401 acatgttcag gaagttttgt tttgttcttt tctgcttcct gaatgaggaa atcaagtacg
     5461 atttcttcgc ctagagcacc cgttttttgt tttgccttgt tgacacgctc ccaatcagta
     5521 tggcgtccag tgaattttcg tttctgagtc ccattcgtgc gatttggtcc cacttcatca
     5581 acaagaacag taaatccttt tgaactttct tgctcaattt cttcaacttc ctcgtttgta
     5641 agatccagtt cagacggcaa cgattgtgta tcatcgattg ggataagatg acgattttca
     5701 atcaatgcaa caaaatcggc ataggtaatt tcattcattc catactgatc ccagaagtag
     5761 gcccatgaat tcggcttttt acgcttcttt ttgctaggtg tattccaatc gaagttttca
     5821 ataaatgaat caccttgttt aattgccggt tcgattgtaa atgcgtgtgt aattactgcc
     5881 gtaacatttc cagcttcatc aggttcagaa atgctctcaa cgtatccgcc cccgtaaatt
     5941 tcccacttac ggtctttagt aattttcccc ggatgacgat tcaaaacaac cgcgccggct
     6001 ttcaatttac catattggcg catgttccaa ccgtaacttt tagcatcatc agcgtatttc
     6061 aactgaccat catcttctgt tttcccgtga tctaaaatga aagcttgttg gctttctgtt
     6121 ggttctggta acaaatcttt aaacgtaacc atatcgatac ctctttctat aattcaatcg
     6181 gaacaaattt atgttctggt tgaattaata ataacacgtt cgatatttta agtcaacaat
     6241 ccggacataa ttttgttccg attaaattta tgaaatcaaa atataatcgt cagattccgt
     6301 cttaccagta ttcttaccga cagcacaata tccaatcggg caacgagccc atacccaacc
     6361 atccgcaatc gtcatcttct ctgacttcgg aagcgtccaa gttgcgcctt tattgtatga
     6421 accaacaacc gcaccactta aacttggctt gttgcgaacg ttgagaccac tcacttgaac
     6481 cacaagagtt ttcacgttgg atgttgatga cccagatgga taaacttgat taccgttctg
     6541 gtcgaacacc ttataaccag aatggctatc tgcagcccgc ttcgcattgt tcaagtcctt
     6601 ataggcacca atttgtgact tggcgtcagc ccatgatttg cgaacacgat acagaccact
     6661 agctggcgtg cttggggttg atgaaccatt attaatcgca ttggctttat ccgcaatctc
     6721 ctgcagttta ccaagcaact gtcccggaca tgatgtggcc gaaaaatgtg agtgagggaa
     6781 gatattcttg ttcaccacca atttccctag accataacgt ttggcaatat ctgcacacaa
     6841 ctttgcactt gcatcaatag ttgcttgatt gataccccag ttcggtgatc cagtgctatt
     6901 gcagtgctca atcccgatac ttgagatatt ggcgttccaa ttaccagcgt gccaagctgt
     6961 gtttccttcg tccacgtatg ctcgaatttc tccatttttt ccaataccgt agtgtgctga
     7021 cgcctcacgc gttttccaaa tatcaggaac aatatcatag ttggtggttg ccatgtgatg
     7081 aatcactatc ttatctactt tggcaccact acgaccattc gagtaatggc taaatgactg
     7141 aaatccatat tttgttgcta gattactaat tcccattttc tgtttcctcc ttcttttcgt
     7201 gtaactgctg caagatttca cgcaactttt ccggcactgg cagtccaatc gccacggcat
     7261 tctccagcag tgaaattcct tcgtttgaga tatagaagaa aatgaccgca tcacgcagta
     7321 catttccacc tttgatgact tccacatcaa gcatatgagc cacaccaacc aaaagaaaaa
     7381 tcatgatttt cttagtgatg ccctttgccc caatcttgct ggatagtttc ttttcgttga
     7441 ttgcacgaag cagaccagtt acatagtcca ctaccacaaa agttacaagc gtataaatca
     7501 aaccatcaat tcctcccaca taccatccaa tcactcctcc accacctgcg atggcgagtt
     7561 gaatccaatt ccacacttct ttcatttaca aatcctccaa ttgttctaaa attaaatcga
     7621 gcatttcagc atctttccct gaaatggtga cttcactatt ttcaaatcca gcaaccaata
     7681 aaggcagctt gtcttgaagt tcatccacgt tgatgtgggt ttcttcgttg agcaagtctg
     7741 ccttttcttt gtgatattca gttgcggttt ctgggatgag tgaaaaggca ccgtcatgtt
     7801 ggaccaaatt gccgtgctca tctttctttc cgtatttttg aagcaagtct acctcgtctt
     7861 gatagagttc atcaaccttc acctggatga gttttgcaag cttcgttcgc actcggctaa
     7921 tctttggtgg taagtcaaaa ccttttaaga aactcatcac gtccaccaaa tcttcatttc
     7981 taattttgag cattatctat cttctccttt aaatcaccaa tctggacaca aagatcatcc
     8041 acgaccatca caagcaaatc aaccatatcc gtcccaactt tcattggctt tggaatatct
     8101 gcctcgatag gttcagttac tatcacttct tctttttctt cgttcatggc ggcctcctaa
     8161 ctgtatgtgg tcattgaatt aaacgactgc ggattgtacc aaccactcgc tgtaccgttg
     8221 ctactgaaac cggttggtaa agcgattgtt cgtcccgcaa gtttggcaca gatttcacgg
     8281 atgactccaa agtcaaccca cttcccatca tctccgagca tcagattaga accgttgaat
     8341 gcaataccag cgctgccgga acttgagcca atcttagtat aaagggaact actaaatgta
     8401 atcccaccaa cagatatttg atgttgtgtc cctatacgaa gcattgatgg taaataaatg
     8461 tcgtcgtcaa agtggaaccc cttctccata ccagactgca atgttctcgc ataccaagaa
     8521 agcttctggg tgtagacact cgcagaggag gaatctttat gagcccatga catatactct
     8581 gttccatact caagatcaaa taccaatcca cgccaatcag agtgaccact gatgttattt
     8641 gcaccaattg ttccgactgt cgttccacca cgtcgaatgg tcattccctg cgagtttatt
     8701 tcagtcacaa gagtgcttcc tgaataatat ccaatcgtag ttcctgtgat tcgaatggac
     8761 gaactcgcag tcaatgctgt taggatatta gcagcaatct tatcagccgt aatcgagttc
     8821 gcgccaatac gggctgccga caaagttcct gtggtaatct tcgccgcatc aatgcttgca
     8881 atttttgcac tttgaattgt ggcatctgca atctttgcgt ttgtgatggc cgcattttgg
     8941 atcatggcag aactaatcac tgcactatca atggttgttt gaccagtgat gtgcaccttg
     9001 tttccagcaa tcaaaatact ttcaggtgag atattgattt gattaatcac gtctccttta
     9061 ccaactcgta aattgattaa gtctttctgc atgagaattg ctgaatataa tgtgttggca
     9121 ttggtatagt cttgccttgt ggtcgtagga taaatatttc cttcatattc atcgggtttg
     9181 atgctcgtgt tcactcggac tcgcattttc aatgtgccac tcgggactgt aattgctccg
     9241 ctcgaaccag tataagagat ttgatgataa cttgaaccat ccgacgggtt tagagtaaaa
     9301 ggtgtgttgc ttgaattata aatcccagca tatgtgccag tcttaactga aaagaaagct
     9361 agatacacgc tgtcagtagt aatccctgat atctgctcag ccgttaacgt ccaagaataa
     9421 gtggcattgc tgctacttgt aacggtcgaa atctgttccc aagcaccacc tgtataaagc
     9481 aagacaatat tatctgatgt atccttgcga ccattccacg aaattacact cggagcgact
     9541 ttgccttcca atggcaattt gtaaatgacc agataatctc ctgaatttga ataggggttt
     9601 gttgtcacgc cacttgtaat actcatcgat tcaacgttct tacttactag ataactcgca
     9661 atggtcgcat tagtataagt tcccgccgtt agggtttcac taaattgttt tgccgttggg
     9721 acaaaatcaa tgtaaggtaa atccatgccg tagtaatgat agtagaaagc agcttgagtt
     9781 ccgtcacgca gttgcccgat atacttatct cccactttga catcaaggaa ctcacttcgg
     9841 agataattgg ttgcggacac ttctgaaccg tcagtggtac tcaaacttcc ttgttcccat
     9901 tgattgactt taaatggaac gccgattgct tctttggttt ctgaaacact taacagtaag
     9961 gcatcagtgg tcgctgtaag ttttacgacg ttacttatct cggataaatc aacatagcca
    10021 tttgtcacat ccgttttcaa catatagcaa ctgatggttt ctggtgcatc actagt
//
LOCUS       DAYYME010000099        10051 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000099 DAYYME010000000
VERSION     DAYYME010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.100"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..213)
                     /locus_tag="ACHBFW_002121"
     CDS             complement(16..213)
                     /locus_tag="ACHBFW_002121"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM9318397.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(359..1237)
                     /locus_tag="ACHBFW_002122"
     CDS             complement(359..1237)
                     /locus_tag="ACHBFW_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9318398.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1276..2394)
                     /locus_tag="ACHBFW_002123"
     CDS             complement(1276..2394)
                     /locus_tag="ACHBFW_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM9318399.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2391..4490)
                     /locus_tag="ACHBFW_002124"
     CDS             complement(2391..4490)
                     /locus_tag="ACHBFW_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFM9318400.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4541..5362)
                     /locus_tag="ACHBFW_002125"
     CDS             complement(4541..5362)
                     /locus_tag="ACHBFW_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9318401.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5359..6168)
                     /locus_tag="ACHBFW_002126"
     CDS             complement(5359..6168)
                     /locus_tag="ACHBFW_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9318402.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6189..6683)
                     /locus_tag="ACHBFW_002127"
     CDS             complement(6189..6683)
                     /locus_tag="ACHBFW_002127"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9318403.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6695..7195)
                     /locus_tag="ACHBFW_002128"
     CDS             complement(6695..7195)
                     /locus_tag="ACHBFW_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9318404.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(7260..9956)
                     /locus_tag="ACHBFW_002129"
                     /pseudo
     CDS             complement(7260..9956)
                     /locus_tag="ACHBFW_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
BASE COUNT         3056 a         2264 c         1655 g         3076 t
ORIGIN      
        1 ggctgaaaaa atctatcacc aaaaaaattc aaacattaag ccaaggatta tgaagtcaac
       61 atttcgaaaa atgaacatac attggtaagc aactataact attaccacag acataaagta
      121 acacaatata ttactattta agtgtacgcc accagtgaat atcctcaagg tatacagcgc
      181 acaacaaaaa gaaaaaagga aacgcagaag catttccttt cagtgattta atgaaaacaa
      241 cacgatttga cttagagcct ttttatttga attcattttc gctttatcca acttcatttg
      301 atcatttgat tactcggtta aatacttttg acatgagtat caatgaaaca ttcattaatt
      361 atggttgctt atttcttacc gcttttctgg ccaatgctaa tccgccgatt attccggcat
      421 tatcacctaa ttcgggcgta ataatgtagc tatcaattgg tggtatttca acatatccat
      481 ttataagctc aacaaattta tcctgaactt ttttcttcat gtgttcttgt ttcattacac
      541 caccgccaaa aataattcta tctggcgaaa acatcagtgt tgtattgtat gcacattggg
      601 caaggtaaaa ggcttcaatc tgccaaaacg aatcgtcagc caataaattc tggcctttta
      661 cacccaaccg tttttcaatc gctggtcctg ctgccatccc ctctaaacaa tcttggtgga
      721 agggacagtt tcctgaaaaa ttgtcctttg gatgtcgctt gatcaacata tgccccattt
      781 ccggatgact gaacccttcc acgaaagtcc cattgaacaa ggcaccacct ccgatacctg
      841 ttcccacagt gaagtatgca acacctgcta accctttgcc agaaccagca acatattcac
      901 catagcaagc cgcattgaca tctgttgtcc attcaatcgg aatattgaaa tattgcttta
      961 gcgttcctac aaaattgaaa ttttgccaag caagttttgg tgttgatgtg atatgaccga
     1021 aactcgttga tttgcattga atatcaattg gaccaaagca gccgaccccg atgcctgcca
     1081 attgttcctt aaaaggttta aaaaaatcaa tgacctgctt cattgtttca actggagttg
     1141 ttgtgggaaa actgactctt tccaaaatcg ttaattcctc agtaccgact gcacagacaa
     1201 atttagtccc tccagcttca attgccccat atatcatcat aggttcctcc cttcgaatat
     1261 gtgaaaaaca cttcattaac gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt
     1321 tcctgaagct tctgttgaat accagtaagc gacactagca acatcgtctt gccgctcaaa
     1381 taagcgaaca tcatcgttgc cgatttgttg taaagtgacc gataaatctt cggtaaaaca
     1441 gatggggtct tgaagatgcc aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa
     1501 ggcatgaacg gcattcaatt ttcccgtttc aaaacgatct cttgtggcat ctctatttga
     1561 ttggtaagga taccctaaaa aggctgttga atagtttttc gcatgtggac gtccgaactc
     1621 atcgtactca tgaaaggccc acgccccgcc aaaataatct tctgaacctg ttgaggttac
     1681 ggtcggatac tcactatccc catcaatgta gaatttaaac tcgccttcgc cccaccaata
     1741 acgttctagg caggtaagcg caaggtaagt acccacgtaa tagccttgac cctttaatcg
     1801 atcaattaac ttataatcct cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg
     1861 aaaatatagg ctattttctt ccaattcgtc caccaaagcg taatttatcg tataaaaaaa
     1921 tgaggacata tccttcggat gtaaattctc gatcgtaatt ttagcggatg tttggaaagg
     1981 catttcgaaa taggaattaa agccccctgt aggattaacc actatcggca acgagttcac
     2041 ctcgcaacgc tctccaaacc cattacaaaa gaaatcccct aaaggacttt caacagcagg
     2101 gctatccgac ccatcccaat aaattcttaa gattaaatca cgtaaaacaa aactgccaaa
     2161 ttctgttcga tcacgaatcg tcatccacat atgacgaatt attcccgttc cagtaatatc
     2221 cgcaatcaca gtttcttcac cagaagtcag cggaattgcg ccacgacctt tgcggcttgg
     2281 tcctagctcg cttggcaaca tcgcagcttt acctttctcg cctgttggat tttcgggagt
     2341 gattgctcga ctctggattt ttttaaattg ggtaatattt tttagtatac tcatcttttt
     2401 ctcctttgaa tgtgaatcgt ttttcccttt tctacgtgaa gaattttttt cgtatcactt
     2461 attttcaatg gcttttctga caaatttgaa atactcaact cggattgcgt acattcaatc
     2521 gaaatgaagg cttgaccgaa cttcaaattc ttccaagaaa acgttaaatt atcaacagga
     2581 tcaatttgca atactccttt tttgaatttc aatccgaaat attgactgat cattaggctg
     2641 acaaaaagac ccgatgccca gccgcatttg ccgcagccaa agtctcgcac gacttctcct
     2701 tgttgagcct ctaagcgata cggccaccac caccacgaac cgtctaaatc tgccaaacaa
     2761 attagttctt ctaatcgggt tacgaaagct tgagggttgt gcagctcgct cattaaaaca
     2821 gtgataaagc caggaaaagt ggcgccggat tctaatcccc aagtgatgcc tttgatttcc
     2881 tcgctatagg ttggattgaa agaactagca gaaaaggcca tggtgttctg atataaatga
     2941 tctttattat ctaaaaattg ataaaaaggc atcaacgtag tgtctgattc ttctccatca
     3001 tgcatcaata aattaatttt tccatccaca atcacatcac ttaaaaagat taacccttgc
     3061 tcaagaatcg gtttttgata gtgcttcact gaataggttg ttttctcctc atcaccgatt
     3121 ccctccaaaa attgctcacc aaaaggacca tcgatagtca tttgtttaca aattgattcc
     3181 tttagtttac aggcaatgtt tttgtatcgc ttgctaagac tcagttgttc tagggcttca
     3241 aaaatttctg caagtccaat acaggccttc caaagacaaa gattgctgcc ggtatgatac
     3301 tttccaagtg caaaggcgtc tgataaccaa acagatggaa aaagcattgg ttcccctgac
     3361 cgacgttgag ctaatatgtc ctcaataatt cttgaagcat tcagtagaac ctgcggacac
     3421 cgctcaaaga attttgtatc cccagtatgt tcaaaataaa gactagccag taacgcactg
     3481 gataacgaat tactcagaga atgattgata cctcctgtaa attttgaacc aggaaactta
     3541 acgccatatt gatcaaacca tagaaccgtt ttttgacata actccggttc aaacatggtg
     3601 aatggcaatg atgcgtaata catatcctta ttccaaattc gattagtagc aggatagctg
     3661 ccccaattgc tgccgacgac ctgtccctgt cgattcatac cgaaactaga aaagctttga
     3721 taaagtgcac gacgatagag atggacaact ctatcgtttg gcattgacag ctgcccaaat
     3781 agctgagcaa aatagttcaa tgtttccttc agccattttt cgatatcaga ttcctgaaaa
     3841 gtcattactt cttcaaaagc atttgggtcc acaaaagcaa tggcgaagct tgccacttcc
     3901 tgttgtgcga tcgtgtcctt atttcctttt tgagaaatca acacattttg ctgatccgaa
     3961 tacttctctt gatacgaagg cacatctttc actgaaactt gaaggctttc gtgtgtttca
     4021 ttcacaatgt aaacctgttg aacaagcata cttaaacgct tattagccag tatgggagca
     4081 aaggatacta caatgacctt aaacgtcgaa taatgatgaa tatatctagg caaacagtct
     4141 tgaatcacat cgacttcaat acgatgatcc gattctgaca aatggtagac aatcccgttt
     4201 acttcaacct ttaaggataa cgaattggtt tgagagagct gtttattgac ccaaacacct
     4261 ggtttttcct cagttagtaa attttctcga taaaaggtca acgtttgaat cgtcccgcaa
     4321 acatcaaggt ttgctaatgt cttttgatta cttacatcga aggtcaatcc ctttacatca
     4381 ggaatattcc gatagtagct gtattcgttt gaagtagtca catttccgtc gtttaactca
     4441 gcattaaaaa atgggtgttt gggtgaataa attacttcat taatagtcat cttttccctc
     4501 cttgctaaaa aaactgcagc ctagtagctg cagcttccta ctatgcgatt ccaagtaatg
     4561 acaataaaat tcccaagacg accattccaa aaataatttt gatgacgccg acttttttat
     4621 ctagtaattt gaaacagctc aatgttacca ataacggtac aattccttta aaaatactat
     4681 cgaggatttc ttggacattc attagttctt cgcccttcat tgaaaagctc aaaacggtat
     4741 tgaatttgac catactgatc gtcattgcac ctaccatgat cagacccaag atactagcag
     4801 cttttgtcag aatttcaatt aacccgttcg catacagctt ttcaatatat ttagagccta
     4861 atgaatagcc taaaacgccg ccatagtatc gcatcaagta attaggtata ttgaacataa
     4921 ctaaaaagat aattggtgcc aaaatatttc cggcattggc taagttgatc gctagtcccg
     4981 cggtaacgac acgccaaact ccccagaaca atgaatcgcc aattccagcc aatggtccca
     5041 tcaaactcgt tttgatcgcg ttgatacttt ccacgtcaaa accagctttt tcagaatttt
     5101 ccttctccat cgatgccgca atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa
     5161 aatactgtgt atgacgttcc agcgccgcag ctcgatcttc ttcacttttg tagaaatgat
     5221 taatgaaggg catcatagaa taggcgaaac caccgccgcc catcgtcacc ggattcaccg
     5281 aagcatacat agtcatcgag cgccaaaaca tcttccgaac caattttttc tcatctttag
     5341 aaattccatc agctaatttc attgaaagaa atcctcctct tcttggtcaa gcgttgcatt
     5401 tacagcagca tcgctttgaa ctgcatgaac agatatattt tttagttgta gatctctttg
     5461 ggctataaca attgcaataa tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag
     5521 atacgccata acaacggccc caaagaaaaa ccacacagca atatttttac tccaaagcat
     5581 ccgtaacagc atcgccatcc caactgccgg cagcagattt ccagcgactg ttaatccgtt
     5641 catgatgacc tctggaatat tatccaagac gcttttgaca acatctgctc caaataaaac
     5701 ggcaaagaat ggaataagag cataaagcgc atagtggaca aaccacataa agaagtgcag
     5761 attgatgatt cctttttgat caccagattt tgcaaatttg aaaaatttag cagccatcgg
     5821 tccacataca aaagaaaaaa tcatattcat taactgtaat cctaatactg ctagtggaaa
     5881 gacaatggga ataatcgcat ccacgtttcc tcccattttg attgcgaacg ctgtagccag
     5941 cccagcagcc aaagaaggct ccgccgtact agagattccg atattgacga cacccataaa
     6001 gatggcttct agtgacgccc cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc
     6061 tgctaacgga gcaacaacaa tcggacgact cagcatggac atcccgaaca tttcatgtcc
     6121 agcaactcca ataaaaacgg ccaaagcgac tacaaatgca tcatacataa tttttctcct
     6181 tcctttttct aaaaatcact caaaatagag atagctgaac gtttttccat cgccggtgta
     6241 ggctgaaaaa cggtatctgg ataaagttca actaattctt ttaagtcttg aatttctgac
     6301 gtcgttaaca tcacttcctt tgataaggtt ttcttatctt ccgatggcgg tgtcattttc
     6361 ccagcattcc ccacgttcat taattcgatt tccgttggga aagctttcgc aacttttacc
     6421 gcatctttga cattagggac taaaaccatc aagcgcatgt ccttcgcccg cggatctttt
     6481 aacaaatgaa tcgcgtcatc aactgtttta ataatgactt tgacattcga tggtgcagcc
     6541 atcttcaatg tcatcttttg aatatcgttg gtagccgcct catcgcttgc tactactaac
     6601 ccggttaaat ttagactctt tgtccaagtc atcgctactt gtccatgaat taatcgttca
     6661 tcaattcgca ctgctttaat catttttctt cctcctaaaa aaaatcttcc tcttcattta
     6721 ctacatgtac ctcatcaaga atggtcatct ttaattcctg ttggcattta tttaccagtt
     6781 cctgaatatc ctcctttgtt aattccttgg ttgccaatac tatttcgagc agaattggca
     6841 gattaaagcc agacaaaata gttacagcaa tctctttctc caaaaaggtt ttggtaatct
     6901 tctgattgac acttccgcca aataaatcgg taaaaacaaa aatctgatcc tgctcgttca
     6961 cctggtttac aaatacattc aactcctgtt caaaatcaca ctcatccaca taagcattaa
     7021 tcaccgtaat ctcttgaacc ttccccgtca gtaattgaat actgctaaga aagcctgaag
     7081 ctaagtgacc gtgagtcgct attagaattt tcttcatatg gttaattcct ttctttctgt
     7141 tcacttttat ataagcaatt cctgtgccaa cttttaaaaa cattggttta atcacgttaa
     7201 ttcacattaa aaaaagtgtc taacctaaaa aaaacagatt agacactttt ttagacactt
     7261 tatttttgaa tatacggtcc taataattca tagagcagcg aaagctcata agcattgact
     7321 ttaaaattat atttcatctc cattgattga aaaatacttc tagaaacata ataaaattca
     7381 tcttcatctg gtttcaaatc ataattcggt tcttcagatt tttctgtccg agccaagaat
     7441 aaacgttcca tcatcaatgc cgtatgcata tagagattta gttttacacg gccatttaaa
     7501 gtgaaatgat agtaattttc atatttagaa atgactgttt ccacttcact catcacgatc
     7561 gttgggttca gaaagttcaa acggtccgca attccttcca acgaaaagaa ttttactagc
     7621 tcctgaatca agcggttaaa ttctgccggc ttcatttcag gactcaaaat atgctccaaa
     7681 ttggctgctc catcaccgtc taagatgtca taaacattaa tattaggaat tgaaaaggaa
     7741 cgtggaagat ccgaagtcgt gatgaccaat tttgtcttgt caaaataagt ttcatcattt
     7801 tggtcgatta attcacgtag ttctctgtag tccttcgtga aaacttcgat ggattcatcc
     7861 gataaatgat gacgaaaaac atctttcaat ttgtccgaaa tccccaaacc cgacatacag
     7921 gaaatgataa tgttgctact ttgagaaaag ccttcaaagt attgaacatt gattgaatac
     7981 ttgtctgctg catcctccgc aatctattta aatggcgcat tcatcgtcat cttcaagcca
     8041 atatcaagtg caatggaagt cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg
     8101 ttcttgatct gtgtgtaaat ttgatttaaa gagcccatat ctacgatcag cactagacta
     8161 tctgtattca cttgccgttt cacgaaattc cttgtcgatt cgacaatctc tgacacatta
     8221 ctctctactg gcatatccaa cgcttcaaaa acataattgc cgcataattg attcactact
     8281 gcttgaatgc tgctagccgt actatctccg tgagcaagca acaggccctt cagctcgatg
     8341 gattcatcta cataatcaga taaagtcacg acaataagca gtcttagaaa gttctcgtcc
     8401 ttttctgaga gcggtggtaa atgctccaag aactgattac ataagtaagt cgtccgaggt
     8461 agatttgcag ctaatgtacg atttatcccg gttaagtctg tcttatagct tggggaaatg
     8521 acataggatc tgctaataat ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt
     8581 cctaaaacgg atacccagac ctttctaaac atctgaatca tcaattttct actcattgat
     8641 aaaaaagaat ccttatcaat gtgatgcacc aaatgctgta cttcattctt tatcgaagaa
     8701 aaattttcat tgaaattacc ttcatttatc agctgagtaa ttttttccaa aatcgaatca
     8761 taatcaaacg gtttgattcg atccgttttt atcttttgat ttttctttat caataaacta
     8821 gcaatcggca agctttctac ttcggattcc agcatattta actgaatcaa gatctcattt
     8881 ttcccaatcg ttaatggttc ttcttgtgtt cgactataca catccgcaca actgatttta
     8941 atgatattct tcagcttacc aatattccca ctaaagggat gagataacat taaagacaag
     9001 gcttctcttg aaacaataat atctttttgt aaaacatccg cttcattttg aaaaaataat
     9061 tgaatcagct ctaatcgttc tcgaatgggg cgatcttcaa aagttggcaa cataacacta
     9121 atttgaatac gccgatcaaa ggtgtctaat aaataatttt cacctttttc cgtcgtggcg
     9181 aaaataaaac gaaccttagc agaatgccaa tttacatttt caccgactgg acgatagttt
     9241 ccagtatcaa taaatagaaa gagcttttct tgattttctt tcgataggcg atggacctca
     9301 tcgagaaaaa gatagccgtt gttggccgtt gccaataaac catcggtatc cttttcagcc
     9361 cccgtaaagg cccccttctt gtatccaaac agtgttgccg aaagcagttc gggattgtcc
     9421 gcataatcgg cacagtttaa gatagcaaat ggtgcatctt tttcaataat ttgctcatgg
     9481 atagcgtatt caaaaatttt tgtggctaaa aaactttttc caacacccgt ggctccattt
     9541 attaatacac tgagtccgtt aggcggatat ttgacagcgg ctatacattt ttgaatgacc
     9601 tctctcagac ttccgtcatg accaactaca gaatgaaagg cttcgtttat ttgctccgta
     9661 ttatctacaa ccgcatattc atctaaaggc ttccacaaaa caggctttcc cggtaatttc
     9721 tcgacctttt tatcttccac taatctcgtc aaataatggc tggcattctg acgagagata
     9781 cctagattct tcgaaatttt ctcggcagtg acagaagaca tattccctgt tagtcgtatc
     9841 agctcatcaa aaactcgatc tttagcgctc tttttcattt tttcctccag tttaatgtgt
     9901 ctaaaagcat aataaactat cggctgcaat atgtctattc tatatttgta ttccaatttt
     9961 tactttgaaa cacaaatagt cgaatatttg cctcagatat ctgacaaaat aatttatcta
    10021 ccaatttagt ttttatctaa tatgattttt a
//
LOCUS       DAYYME010000100         9812 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000100 DAYYME010000000
VERSION     DAYYME010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.101"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..3308
                     /locus_tag="ACHBFW_002130"
     CDS             30..3308
                     /locus_tag="ACHBFW_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFM9318405.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3849..5756)
                     /locus_tag="ACHBFW_002131"
     CDS             complement(3849..5756)
                     /locus_tag="ACHBFW_002131"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFM9318406.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5886..6761
                     /locus_tag="ACHBFW_002132"
     CDS             5886..6761
                     /locus_tag="ACHBFW_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFM9318407.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6841..7815
                     /locus_tag="ACHBFW_002133"
     CDS             6841..7815
                     /locus_tag="ACHBFW_002133"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM9318408.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            7995..9140
                     /locus_tag="ACHBFW_002134"
     CDS             7995..9140
                     /locus_tag="ACHBFW_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM9318409.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9277..9621
                     /locus_tag="ACHBFW_002135"
     CDS             9277..9621
                     /locus_tag="ACHBFW_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFM9318410.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
BASE COUNT         3195 a         1803 c         2070 g         2744 t
ORIGIN      
        1 ctttctaatt tgttacggga ggaatacact tgttaaaaaa acgtataaaa tacagtgcgc
       61 taatgatgaa tctccttatg ctcagtaccc cggttttaat ttctgtcgtt gaagttgctg
      121 ctaacgaaca acagtctgtg aacgaagaaa atgcacacgt gaacattgaa gggatgtcaa
      181 caatggagga aataccaaga actagtgaag atatggatac ggaagaagga aatgatccta
      241 tgatgaaaga atcagaaaat gaaaagacaa aaaagcaatc tgaagaagca aatgaagagt
      301 ggaaaggggt tgttttcgga gaaagcacaa gtgctagtag gtatgccatt gaaccattag
      361 aagacggcgt gcggctctct tccacgaata acggcggaaa attccagtca agcggatcag
      421 atggaatgac ctattattat caagcaatcc ccaaagacat caactttagg atgcgggcga
      481 cgatcgaaat agaatcttgg acctatacga atgcccaaga aggatttgct ttaatggtaa
      541 gagacgcagt accaaaaaat catttgtttg ggcaggcatt ttattcaaat tcatttgcca
      601 tactaggcag tcggatcgaa tatgtctggg actcggatag acaagaagtg gtcaacacga
      661 gcggcagcaa atatacgatg cgacttggat tggggacacg ggcaatcaca ggaatatcat
      721 ctgaagatcc gcaagctgcg ccagctcctg ggagtgtctc agtggaaaca acacctttag
      781 agacttcagc agggttcttg gaaaaagaaa caggttctta taatatattc ggaaacggtc
      841 atgggaatct gtttccggcc acgaactcga ttactaaact ggacgtagag atgaaaaaaa
      901 cgaatactgg tttagaggca tattattacg atcctgaaac aggagaagaa atggcttctg
      961 acatcatgta tgactgggaa aaattatacg cttcagatga atcggttatt tatgcaggat
     1021 ttgctgcagc aaggaatatg acgatcaagg tagaaggaat cgaagttgct tactcagatc
     1081 ctgcgacaga tcctcctggg caagaaagac ctactcgctt gattgatccg atttacactg
     1141 tgacctctag taatcattca ggaaaccaag atcatactat gtcctttaga gcaaatgctg
     1201 acggattatt gacaattaaa aataaagcaa caggagaggt gcttaagaac gcaaaaaact
     1261 tggctatcac tgcgaatcgg gaatttgatt atcagacaaa actgatggaa ggaacgaacg
     1321 aatttacttt cagttttacg ccagataaaa actatccgcc agaagaatac gtagagatga
     1381 ctagttatga aacaaaagag attcttcata cagtggacta ccgtaaacct aaatattcga
     1441 cagtttatgt aacaccagaa gggtcagatg caggaaacgg aagcagacaa aatccattat
     1501 ctctgacaca agcattgaga tacgcatatg ctgggcagac aatcgtcatg gcgcctggaa
     1561 cttattcctt cgaaagagga ttgacgattc caaaaggcgt taacgggaca agagaaaacc
     1621 cgattcagtt gattgcagag aaaaatccag aaaacaagcg tcctgtactt gattttaaag
     1681 gaactggaaa tgggatgaca ctgttcagtc actactgggg tttacgaggg tttgacatta
     1741 caaatagtgc agctatgcaa aaaggattac agatttctgg aaatcataat ttgatcgaag
     1801 agattcatgc ttatcggaat ggaaatacag gaatccaaat cagcggtagc tcaaatgatc
     1861 ctttcgaagc atggcccgcg cataacttag tcaagaactg tacatctttt gaaaatgccg
     1921 atccaggatt tgaagatgcc gatggatttg cggccaaact gacaatagga gaaggaaatg
     1981 tatttgacgg ttgtatcgcg tatcataatg cagatgacgg ttgggacctt tttgctaaaa
     2041 atgaaacagg ttcgatcgga aaagtaatca tcaaaaactc agttgcttat cgaaacggct
     2101 gggtaccagg aattgaggga gaaggtaacg gtaatggatt caaaatgggc ggttcttctt
     2161 taacgggccc tcatgaattg atcaatagtt tgtctttcga aaatcttgcg aaaggaatcg
     2221 attccaatag cggaacagat attatcgtca atagcagtac aagttttaat aacggttcat
     2281 ataatgtggc tctttatacc tcttcagcag gaaacacaga ctaccatgct tctggtatct
     2341 tatctttccg aacagagaat cttgagatga gagagcagat ccgcccattg aatcaaaacg
     2401 aagatcaaat ctaccactcg ttgaactact attggaatga acgagagaaa caatcagaaa
     2461 atactcttgg acataccata tcaaaagatt ggatcgagtc tctaaataca tgttcgaaag
     2521 aaggtcagca gcctgttacg cgctatgaaa atggaagtat ccatgttcat ggactgatgc
     2581 agattaaacc ggggaatcgt caaacagaaa aggaaagagt ggatggtctt ccattatctg
     2641 taggtgctat ttttgacgga gaaacctcac cgtcttcgga atatccagag tatgcaatcg
     2701 tacaagaacc aaatcagaca aaaccaaatg aacaaaataa tccgattatt atacaagaag
     2761 caaatggtgt ggcggagaat gcagaaggaa atatcattga actgaagcct tttacagttt
     2821 ttgtgtcaac tttcacacca aatcaaggaa cgcctgtctt tgaagatatg cgttatttcc
     2881 cagaaaagat ccaaatcgtt gaaacacagt caagtgctca attttctaat cataccgcta
     2941 ttttttcagc accttcaaaa ggagaatata cgctagaagt cacttatcgt tcggaaatat
     3001 ttgatgggga aaaatggtct gctcatacag aatctgttac ccagcaaaaa gcgatcactg
     3061 tgcgagaaac tgccgatgtg cccccgggaa atgacaaacc agaaagagaa aatccaggaa
     3121 atgaatcacc aaatggaaaa ccaaatggaa aaccttctca tccggatagg acagcaaaag
     3181 aaagtgaaaa aaatggcact caaggactcc caaaaacggg agaatttaat aacccgttgc
     3241 tagcattagc tggaggaatt ttgttaatcg gtgtagttgt ttatgtcatg aaacagcgaa
     3301 aaaaataagt ggaatttctc cttttatgat gtaaaacaat tgactgagga aagtaaaaat
     3361 agaaggaaat gagaccaata gatgtctcac ttccttctat ctttttatag atgaggttct
     3421 ccttgctgct tttgattggc tgttcttatt acttgatgca ggacagcttt ttcacaatct
     3481 ctccactaaa cggtgttcaa tcagctgacc ctcggtatta gctcaacaaa cggcaaaata
     3541 tgaggaacta tttttccgtt tttttctcta atactcaggt caggacgctg atttcacaac
     3601 ctctcgataa acggtgttca aaagccagtt tctcggtagt aagtcaaatt tatgcaaaaa
     3661 tgtggaaagc cattttcaca aaatttttct tactacttga aactaagcag ctttttcaca
     3721 acctctaata catggtattc cagaagtaac ttcttcgaaa aataagccga aatgtcacaa
     3781 aaatttgaga agcgattttc ccaaattcct tcttatttct tgaagctgac cacttctggc
     3841 acaacctctt accagattcg cactcttttt tctggcgcta gatacatagc gtcttgttcc
     3901 gtgataccaa atgtttcata gaattcgtca aggttttttg gttgcacgtt tgctcggagt
     3961 ttagcagggg catggacatc gatttgcagt aaaagttgtt ggtattcttt ttttgctttt
     4021 gtacgccaaa tcgttgccca attgacaaag aattcctcga tagaaagatc tgattccgtt
     4081 tttgctgctt ccaatgcaca gcttagaccg cctgcatcag caatattttc cgaaacggtt
     4141 aattttccgt ttacttttcc gtcagcaaaa ggcagaccat cgaattcttc gatcattgct
     4201 tgtgctttgt tttgaaattg tgtaagatct tcttctgtcc accagttgtt caagttgcca
     4261 tattcgtcaa ataaagcgcc gttattatca aatgcatggg aaatctcatg agcgataaca
     4321 gctccgattc caccataatt agcagagctt gattgttcta gactgtaaaa tggtgcttga
     4381 aggatcgctg ccggaaagac gatcacattc cgaaatggat gataataagc attgaccgtg
     4441 tcagcactca tttcccattc tgaacgatcc actggtttgt tccatttgct gaaacgatct
     4501 tttaatgtga gtttgctgaa aacaagagta tttgaaagca aagtaccacc ctgatctgca
     4561 ggtactgttt tgaactggtc gaataatgct gggatcttat cggggtagcc tacttggata
     4621 cctaagttat ttaattttat gacggctttt tcttttgttg catccccaag ccagtcattt
     4681 tcttctagac gttttttata aacggcgatc atttttttca ccatttgttc tacgtctttt
     4741 ttagcagctt cgccaaaata tttttttccg tagtaatcgc caactacttg atcgaattgt
     4801 ccagaggcaa ggtaatacgc tgcttttttc tgaggcattg cttcgtctgt tccagacaat
     4861 acgcgagaat agattccgct gacttggcgg aattcttctg atagataacc actaagagaa
     4921 cgtactgttt taatgatcat ccagcttttg aataacggaa aatgagcttc tgtcaggatg
     4981 tcattcaaat gatcgaagta cactggatca gttacgatca ctttatctgg agttgtccca
     5041 atcaaacccg tgacaagttt tttcaagtcc agtttatctg tgtatttaat aaatgtatca
     5101 aaagattgcg gattatacat tttgctgtaa tctgcgtttt cttctgcact ttttacatga
     5161 ggagcaatca atttatcaaa ttgcactgct tgttccgtga ttgcttctgc ctgacttttt
     5221 tctttgccgg caagctctaa taactgaaca gccatatcaa agaaaacgcc tattagttgt
     5281 gcaccgttgg gatggtctga tgcataatac gtcttatctg gaaggaataa agaaggcgga
     5341 taagcaaaga aggcgttcgt ttgcgcattc ttcatatctg catctacatc cagattaaat
     5401 ggcaatggta atgtatccaa cagccattct ggaaattgct tattcaattc gtcgaaagac
     5461 tgcaatgatt caactttttc gattataggt tgaagcggtt gagcatgcaa ctcatttctt
     5521 cggtcataat cgtttgccag ttgataatat gcaaggaaat gtttcatttt ttctgaagac
     5581 actttttctg gatgggcaag cagatcgtct gtttctccca tcaaaagttg gtcgatcccg
     5641 tcaactaaat cttggaaacc gcctgtagct ggtttatctg cagggatttt tgctgttttt
     5701 tgccattctt cattgactgc ttcatagaaa tcttgtttca atcgttcttt gttcattcat
     5761 cgatcgctcc ttttttgttt cgtgtgccca ttatcttaca aaatgaagga attaacaaat
     5821 agaacataaa aaattgaaaa ttcgaacaat caaactgatt tgtttttttc aatccttcac
     5881 ttataatgaa aaaaaggagg cgatcccaac tgaatcaagt agtggataaa cctttatatt
     5941 tcaaagtaga taaaaaaatt aaaaaattag ccagcactca gcagctgcag tcaagaaaaa
     6001 gtaaattgct ttttttagct ctggtttttg aagatcaaag ttatgtgatt atcgatcaat
     6061 caggacatcc ggtagaatat tctccagcaa aatatactta tcaagagggt ctttcttgta
     6121 aaaaatggaa gctgatcaat gaagagccaa tcgagttatc tagatggatc aacagaaaag
     6181 aagatattcc cgttttgatc gaagaaaaaa ggtcaggaaa ggaactcgca aattgctggg
     6241 tgggattgcc agaagaacgt tttttgaggt ataagaaatg ggctaccccc tcaggctatc
     6301 tttgtggtac ttacgcggca gcagttctcc ttgcctatta tcaagactat cgtaaggggt
     6361 ggatgcttcc aaatgagatt cgcaagaaaa atacggctga ttcgttggtt ttgacgaagg
     6421 cattgcgtag ccagatccaa ccactaggac ttcctacgat tccttttcag gttagtacag
     6481 ggatttctag ttttttaaag aagaatggca atcatgagcg tgcacgagca actcttctag
     6541 gttcatggca gcgagctaca aaacgtatac gtgaaggcaa acctgtgatg attggtattt
     6601 tgaaagtact tggcagtaca tatggcaatc attgggtgac agcgtacgct tattttgaaa
     6661 ctgaaacagg agaacgttat tacaaagtac acgacaactg gggcgattat cataaagtca
     6721 ttcctgccag ttggagtaac ggaacagttt cacttcctta agaatttgct gaaattaaca
     6781 gaaaatcttg gaaaaatatg ttaaactatc gaagaataga gaagttttgg ggaggactac
     6841 atgagtaata agtatcagga tgacacgttg aagattttta gtttgaatgg caaccgtcca
     6901 ttagctgaaa aaatcgcgaa agtgttcgga acagaattag gtaaaagcgt cgtgaagcaa
     6961 tttagtgatg gcgagatttc catcaatatc gaagaaagta ttcgagggga tcatgtgtat
     7021 atcgttcaat caacgaatgc acctgtcaat gattactata tggaactact gatcatgatc
     7081 gatgcaatga agcgagcaag tgcgaaaacg atcaatgtcg tattgcctta ctatgggtat
     7141 gctaggcaag accgcacggc gaaacctcat gaaccgatca ctgcgaaatt gatcgctaac
     7201 ttgatcgaag aagcaggggc aactcgagta ctgactttag atctgcacac ggttcaagta
     7261 caaggattct ttgatattcc tgtagataat ttatttacga tgccgttgtt tgctcattat
     7321 taccgtcaat taggtctgac gggagatgac atcgtagttg tttcgccaaa aaacagcggt
     7381 gttcagcgtg cacgtagtct ttcagaatat ttgaacagca cattagccat cgtagatcat
     7441 gcagatgatg cttcaactga gggtggttat gtgatcggtg atgttcaagg aaagacatgt
     7501 atcatggtag atgatatctt gaacacagga gcaacgcttg cacgagcagc aaatgtatta
     7561 aaagaaaacg gcgcaaaaga agtatatgcc tgtgcgtcac atggattatt gtccgatcat
     7621 gcgaaaagta ttttagacaa tgcaccaatc aaagatattt gtatcactga ttctgttttc
     7681 acagaagaaa atcgtcatcc agaaaactta acaatcgtta catgttcaga attaatgggt
     7741 gaagcggtca aacgcatcca tgaaaataca ccgatgagtc cgttattccg tctggaagaa
     7801 aaagaattcg aataaacaaa aaaaagaggg aaagaagttg tgacagaagt gagcagcttc
     7861 aaggagctgc gacaagactt ttgtcacagc tccttttttc tatgcccatg aaacgtcctc
     7921 tagacaaaaa tgagctcttc tttggtttgt tcgctatttt atgctagaat gtgcctagca
     7981 ataatttgga agggatgaat gaagtgaaaa ctatttatct ggatcatgcg gcaactaccc
     8041 ccatgcatcc acaagtaatt gaagcgatga cggcaatcat gcaagacacg tttggaaatc
     8101 cttcaagcat acatggattt ggtcgccacg cgcatgaaaa attggaaaca gcaagacaaa
     8161 tcattgcaga cagtctgcaa gcaaaacctc acgagatcat ctttaacagt gggggaacag
     8221 aaggggataa tacagctatc ttagaaacag cgttctcgcg tcaaaaagaa ggcagacatc
     8281 taatcactac agctatcgaa catcctgctg ttcttaatac catgaaatat ttagagacaa
     8341 aggggtttga cgttacatat ctgcctgtag atgaaaaagg gaatctgtcg atcgaagagg
     8401 tcaaaaaagc gttacgagaa gatacgatct tggtctctgt gatgtacggg aacaatgaaa
     8461 tcggaaattt gatgccaatc aaagaaatcg gtgaactttt agcggatcat ccagcttatt
     8521 tccatacaga tgcggtacaa gcgtacggaa accaaacgat ccatccagaa gaattaggaa
     8581 tcgatttatt aagtatctct gctcataaaa tcaatgggcc aaaaggcgtc ggtttcttat
     8641 ataaacgaga cggagtaaat atccctgcat tgcttttagg cggggagcag gaagaaaagc
     8701 ggcgtgcagg tacagaaaat ttagcaggga tctggggtat ggcaaaagca gttgaactgc
     8761 tgtcacctga agaacgtgaa gcacgtaaca acaaatataa aggattccag caattgattc
     8821 ttgatacatt agaagcaaac gaaatccctt atcaagtaaa tggtgattta acgaataaac
     8881 tccctcatgt gttgaatctt cgcttaattg gcgaagcaaa tgatttgcta ctgatgaagc
     8941 ttgatcttaa tggggtcgct atttctactg gttcagcttg tacagcagga aatatcgagc
     9001 cttcacacgt attagaagca atgtatggag aagatacacc gatactaaaa gaatccatcc
     9061 gtatcagctt tggtttggga aataccgaag acgacatcca gtatttcctt gatcaattag
     9121 ttcgtgctgt caaaaaataa ctagtgaagt cattcttata tgaacagatg tctgagagaa
     9181 cgccttcatt ttttagggaa cttaactgaa aaacctagta aaaatcagga taaactttta
     9241 taataaagag aaactaattt tatcgaggtg aaaatcatgg catttgaaac aacagcaacg
     9301 gtactgggcg caccaattag ctatcgtttg catcctgatg caaaaagata tacattgcgc
     9361 gataatggat ttacagaaac aaacggaggc aattatcaat tgatccgacc attagatgcc
     9421 acgccgcaaa gcaaagaagg attcaaatta aaaatcactg tatcaaaaga tattcaaaca
     9481 ttgaaaatgt cgatcactac agcaaatgga ttgaaagcag tcaatatttt caaagatcca
     9541 aaacagaaaa tgagccaaga taaattttat tttctgatgg atgggatgat tagccgtggg
     9601 ttattcgaaa aagtcgaatg atctgtggat ataaaaagac aactagtttt gaaacggcct
     9661 attgttagat gactaacttt gtaaggacga cgcaaattag ttgtcttttt tatttttaaa
     9721 tttaacacat gttgttattt ttaatatttt gctgatttat tgtttctaag taaagaaatt
     9781 taaattgaaa cgattgttat ttagggaaaa ag
//
LOCUS       DAYYME010000101         9779 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000101 DAYYME010000000
VERSION     DAYYME010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.102"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            332..1381
                     /gene="trpX"
                     /locus_tag="ACHBFW_002136"
     CDS             332..1381
                     /gene="trpX"
                     /locus_tag="ACHBFW_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9318411.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            1378..2259
                     /locus_tag="ACHBFW_002137"
     CDS             1378..2259
                     /locus_tag="ACHBFW_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9318412.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            2262..3032
                     /locus_tag="ACHBFW_002138"
     CDS             2262..3032
                     /locus_tag="ACHBFW_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9318413.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            3311..4165
                     /gene="aroE"
                     /locus_tag="ACHBFW_002139"
     CDS             3311..4165
                     /gene="aroE"
                     /locus_tag="ACHBFW_002139"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFM9318414.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            4343..5506
                     /locus_tag="ACHBFW_002140"
     CDS             4343..5506
                     /locus_tag="ACHBFW_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM9318415.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            5623..6033
                     /locus_tag="ACHBFW_002141"
     CDS             5623..6033
                     /locus_tag="ACHBFW_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9318416.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6207..8435
                     /gene="topB"
                     /locus_tag="ACHBFW_002142"
     CDS             6207..8435
                     /gene="topB"
                     /locus_tag="ACHBFW_002142"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM9318417.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGGHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            8606..8890
                     /locus_tag="ACHBFW_002143"
     CDS             8606..8890
                     /locus_tag="ACHBFW_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318418.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9050..9286
                     /locus_tag="ACHBFW_002144"
     CDS             9050..9286
                     /locus_tag="ACHBFW_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM9318419.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            9283..9681
                     /locus_tag="ACHBFW_002145"
     CDS             9283..9681
                     /locus_tag="ACHBFW_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM9318420.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3399 a         1654 c         2072 g         2654 t
ORIGIN      
        1 aaaattcgat tagacttaaa tcaattctta aggaaagggt gacctgtaat gaacttatca
       61 aaacgtattt cagcagaaaa caaaaaaaat tggcgcagct ggcgtagata agttgtatta
      121 atggtgagac tttcccatag atacaacttt ttagagtgca tttctaaagc ttgtatctat
      181 gctagacatg aacattgact gccgcataga ttagaatcta tgcggttttt tgtgtaaaga
      241 caatgaccgc tttggccatt gtcttttttt gtagactgga tacttgcctg attataataa
      301 ataaaaagat tgactttgca ggaggaaaaa aatgaaaaac aaacgattaa taacagttgt
      361 agtaattatt ttagtttatt tagcaggtgc atttatttat ggaaaagcct caggcgaaaa
      421 agaaagtact tcgacgaacc agacaaaaaa aacaacagtt gttggggtat tgcaatacgt
      481 gagccatccg gcattagatg agatttatcg aggaatcaaa gatggtctga aagaagaagg
      541 attaaaagaa ggagaaaatc ttgagatcaa atttcaaaat ggacaagccg atcagagcaa
      601 gctagccaca atgagtcaac agctagtaca atctgatcca gatgccttga ttggaatcgc
      661 aacacctgcc gctcaatcat tagcaaacac gacttcatcg atcccagttg tgttaggtgc
      721 tgtaacggat cctgtggggg ctggattagt cgcttcttta gagaaacctg gtggtaatgt
      781 aacaggtgtt tctgatcagc cgcctgttac ttcacaaatc aagcttgctt ctgatttgtt
      841 gccaaatgca aaaaaagtcg gaatcttgta tgcttcttca gaggataatt caaaatacca
      901 agtagcacaa gcagaagaag ctgcaaaaaa agaagggtta gaatcagtaa aatatgcggt
      961 gccttccact aatgagattg cacaaacggt tcaagtgatg agccgacaag tcgattttat
     1021 ctatgttcca ttagataata ccattgccaa tgcgatgcaa acagtggtaa aagaagcaaa
     1081 caaagcaaat atccccgtca ttccatctgt ggatacaatg gtggaacaag gtggactagc
     1141 aacaatcggg atcaatcaat atcaattagg gcttcaaagc ggtaagatgg cggcagaact
     1201 tgcgagtgga aaagaaaaac cggaaacgac gcctgtttat acatttgatc aaggggatac
     1261 agtgatcaat caatctcaag ctgatcgctt aggaatcatt gttccccaat cgatgaagga
     1321 aaaagccaaa atcattacag atgactcgca gcaagaaaca tctaaggagg atgacaaatg
     1381 atcgtatcag ctatttcaca aggcatgctt tgggcaatat taggattagg gattttcatg
     1441 acttatcgga tcttagactt tcctgatatg acgacagaag ggtccttccc attaggtgct
     1501 gctgtctgtg tgacagctat tactcatggg gtagcgccga ttgtagctac attattaggc
     1561 gtaggtgctg gcatgatagc aggactcgtt acggggttac tttatacaaa aggaaagata
     1621 ccggttattt tagcagggat tttagtgatg tcaggattga actcagttat tttgtttgtg
     1681 atgcgaacac caaatctttc tttattgaat catccgattc tacaagacag cttccatcgc
     1741 tttgggttac ctgattactt tgacacagtc cttcttggct tcatctcttt gggcatcttg
     1801 attgctgtac tgctattctt cttcaataca gatttgggtc aaggctatat tgcgacagga
     1861 gacaacgaaa cgatggctcg gtcgttaggg ataaaaaccg atcgtatgaa aattttaggt
     1921 ttaactttgt cgaacggtgt cattgcatta tccggtgcgc tgattgccca gaacgacgga
     1981 tatgcagatg taaataaagg gattggcgta atcgtcatcg gtcttgcttc catcatcatc
     2041 ggtgaagtga tcttccaaga attgacgcta gcagaaagat tgattgctat tgttgtcggc
     2101 agtatcattt atcagctatt gattttagta gtaatcaaac tagggtttga cacgacttat
     2161 ctgaagttgt tctccgctat catcttagct atttgtctga tgatcccgca attgaagaaa
     2221 gcactgaact taaaaacggg ttttgagaag gaggcatagt aatgactgat atattgacaa
     2281 tcaaaaatgg cagaaaagtc gtaaacaatg gaatgaatga agagaaagta ctattggatg
     2341 aaattaacct tacaatcaga gaaggggact tcatcactgt ccttggcggg aatggtgctg
     2401 gaaaaagcac actgttcaat acgatcgctg gtacgcttca actcagtcaa ggatcgatct
     2461 cttttaaaga tcagctgatc acaaaagatt cagaagaaaa aagagcacga ttcctttctc
     2521 gagttttcca agaccccaag atgggaacgg ctcctcggat gacagtagca gaaaatttgc
     2581 tacttgcaca aaaaagaggt caaaaacgga aattccgttt acgtaacttg aaaaatcaaa
     2641 aagaagattt tttcagacta tgtcaagaag tcggtaatgg cctagagcaa cacttggata
     2701 ctccagcggg tgaattatct ggtggtcaac gccaagcatt aagcttgctg atggcgacga
     2761 tcacagctcc tgagttacta ttattagatg aacatactgc agctttagat cctaaaacag
     2821 ctaaagcatt aatgcgatta acaaatcagc ggatcaatga acaaaaattg acttgtctca
     2881 tgatcacgca tcgaatggaa gacgccttga aatacggaaa tcgtctgatc gtactccaaa
     2941 aagggaaaat cgttcaagat atttctgaaa aagaaaagca acatttgaca atgggcgacc
     3001 ttttgcagtt ctttgaagaa gtagtggatt gatttcgaca gaaaaattcc tttcagtaga
     3061 aggacttgaa aactcattga catcttcctt tgattcatcc tatacttaaa acaataaaca
     3121 attaaacata tttgggtgtt ttgtaggatc acgtagtcaa tgattttaac aattaaatag
     3181 aatattcttt gctctgacaa gtaaacgatg agaaaagaat ttgctacttt gccacctaga
     3241 cgtttcgtac tttttcgtat caaacgctaa gtggcttttt ttattgaagt aagaacaaag
     3301 gaggaataag atgatttctg gacaaaccaa attagctggg ctctttgctg atccggcttc
     3361 tcacagttta tccccattga tgcacaatac tgcttttgaa gcaaacaaca tcgatgcggt
     3421 gtatttagct ttccaagtaa atccaatgaa tttgaagcaa gcagtcgaaa gtattcgaac
     3481 ttttgatatg ttaggtgtaa atctttcgat gccgaataaa acagcggtta ttccttatct
     3541 ggatgaggta agccaggaag cgcaattgat cggagcagtg aatacgatcg tgcacgaaaa
     3601 gggtcgtttg attggctata ataccgatgg tgcgggcttc atgcaatccg tgcaagaagc
     3661 tgggatttcg atcaaaaaga aaaaaatgac tctactggga gctggaggtg cagcaaaggc
     3721 agtcactgtt caagctgcgt tagacggcgc tgaggaaatc gtagtttata aacgaaacaa
     3781 ccaaacattc gctcaagtaa aagaaacatt cgaacaactc agctatcgaa cttcttgtaa
     3841 gataaaagtt cgtgactttg ccgatacgca tcaattaaga aaagacttga aagaaagctg
     3901 tcttctagtc aatgcaacag atatcgggat gggaaagaaa atcaatcagt caccgctctt
     3961 agatataagc atgatgcatg agaatctagc ggtttttgat ttgatttatg cacctagaga
     4021 aacacgttta ttaaaagaag caaaaaaagt aggagcaaaa ggatacaatg gacttggcat
     4081 gttgatctac caaggagcaa tcgcgtttga attatggaca aaggaaaaga tgccgatcca
     4141 tgtgattgaa aagctgtttg attaaaaaag gataatagat aaagatcttt tattctcgca
     4201 taaacggtct caagaaaagt aatcctttat gtatcgaagc atttatttcg ttatttttta
     4261 ttaaattatc aaaaaatgat aggcttttgt cgattggtta tcgtatcctt tatttatcag
     4321 taagaaacga ggaggaacca caatgagtcg gatggatcgc tataaggaca tccataagaa
     4381 ggcgaaacct ctgaagcggg atacggggag tatttttgct agaagagaac gaaaacgaac
     4441 gcgtatggaa aatgaggaag atgctacccg tatttatgaa agagagcagc attcatttga
     4501 acatttgaac caatacgagc aacagatata cagagatgca ccaagagcag aagaacaaca
     4561 aaaaaataaa aaatttttca atcggcaaaa gaagaacaaa ccgttaaaac ctcgaagaaa
     4621 gcgatcgtgg aaaaaaatca ttctatcgat tttgcttgtt ttgatccttt attcaggtat
     4681 ctcatttttt attggtaaat atgtagctga acatgacaca agcttagagg cagcgacttc
     4741 agaaaccttt catgggcaaa aagcgacgaa tggtgcgcat aatattttga tccttggaag
     4801 tgatacacgc ggagaagatg ccgggcgagc cgatacgatc atggtcttac agttggatgg
     4861 tccttcccat aagccgaaac tgatttcttt tatgcgtgac agcttcgtta cgatcccagg
     4921 ggtaggacaa aataagatca attcagctta tgcatacgga ggagccgatc tagtgcgtca
     4981 aactttagta gaaaattttg gaatcgattg tcaatactat gcgaaagtcg atttcaaatc
     5041 gtttgaaaaa gtgatcgatg ccttattcat gaacggagtg aagatcgatg cagaaaaaga
     5101 tctgaatctg gatggtgtag atattaaaaa aggggtccag aagatggatg gacatgttct
     5161 tttgcagtac gcacgtttcc gaatggatga acaaggagac tttggccgtg tcagacgcca
     5221 acagcaagta atgaacgcta ttttcagcca attgaagaat ccattgaatc tgatccgtgc
     5281 accttatgca gctggtaaag ctttaggata tacatccaca gatgtcactg cttggttctt
     5341 gacgaaaaac ctcttttcga ttgcacgagg agctggtggc attgatcgaa taagtgtgcc
     5401 agtagacggt tcgtggaatt atgggcaaag tgattatgct ggcagtattc ttgaaattga
     5461 taatcaaatg aatcaatctg ctattacaga ttttttgaat aaatgatcat aaaaaagatc
     5521 aagaagaagc tgcgcaaggc ttttgtcatg aatcaatcaa acgattcact cgtaaaaaac
     5581 gctttcttat ggtagattta accaagagag gagctgttta cattggatat ctatctgaac
     5641 ttcaaaaatg agtcgcgtga agccatcgct ttttatgaaa aagtttttca gactgaagca
     5701 tcaggtatca ccacatacgg tgaaatgcct ggaatagaac aaatgaactt gccagaagaa
     5761 gcaaaaaatc taatcacgaa tgcacggctg aacatttatg atacattagt tatgtttgca
     5821 gatacgccag aaggaattgg tcctgaatta gttgtgggaa ataatgtttc tttagtcatc
     5881 cagacaagaa atgaagaaga aacgaagcgc ctatttgaaa gcttagctga gggtggtaag
     5941 attgagatgc cactggatgc tgttccgtgg gctcagttgt atgggatggt aactgatcga
     6001 tttggcatca actggcagat taaccaagag tgacagctaa agatgaatat tttctggaaa
     6061 catcaagcag caaacggatc atcgtttgtt tgcttgatgt ttttgtgtct atttcgacta
     6121 tacggtatct tttttttatt gccagatgcg aaaatatgtt ccataataga gaagtccgca
     6181 aaaaattaag taacggaggt cagaagatga agacggtgat actagctgaa aaaccttcac
     6241 aagcaaaagc ctatgccgaa accttccaac agcaaaagcg gcatgaaggg tattatgaaa
     6301 tcagcgatcc attgtttgct ggtgaagtaa ccatcactta tggttttgga catttggtag
     6361 atatggttgc tccaggagct tatgaagagc gttgggcaaa atggtcttta gaaaatttgc
     6421 ctattttccc agaaaccttc cgttacgaag ttccaatgga caaacgtgca caattttcga
     6481 tcgtaaaacg agaattgcag tcagccgata ccatcattat tgcaacagat ggtgatcgag
     6541 aaggtgaagc catcgcgtgg tcgattattc gacaagcaaa cgcattttct aaggaaaaga
     6601 actatttacg tttatggatc aattctcttg aaaaagaagc aatctatgaa ggttttaaga
     6661 atctccaacc tggaaagaac tattttccaa aatataaaga agcgcaagca aggcagaacg
     6721 ctgattggct gattggaatg aatggaagtc cattatatag cttgctactt caacgaaaag
     6781 ggattgatgg cagtttttct cttggtcgtg tacaaacacc gacactctat atgatttatc
     6841 agctgcaaga agaaatcaaa cacttcaaaa aagaaacgta ctttgaagga gaaggtttaa
     6901 ttacgacaaa caaagggcag ttttctggaa aaatcgtacc aaaaaaagca tttaaaaccc
     6961 aagaagagtt aacgaaggaa atagaaaaac ttggagggca tttaggtaag caaaacggac
     7021 ggattcttga agttacgaaa aaggaaaaga ggttgcatag tccacgttta ttttcgctat
     7081 ctagtttgca gacaaagatg aatcagctaa tgaaagcaag tgccaaagct acacttgatg
     7141 ctgctcaagg attgtacgaa agcaagtttc taagctatcc aagaacggat tccttttaca
     7201 ttacagaaaa tgagcatcag tatctggtta aacatctgca aaattataaa aaatttttag
     7261 gaatagaaga agtacagaca actgagatca aagcaaagaa acgatatgtc gatgcgaaaa
     7321 aagttcaaga acatcatgca attattccga caaagacgat tccgactcct gcacgctttg
     7381 cgaagctttc caaattacag caagcaatct atttgcaagt tttgcgcact actgtggcta
     7441 tgtttgctgc agattatatc tacgaagaga cgaccgtaat gacgggcgta cagcaattaa
     7501 agctaaaaag catcggaaag atcccattac agcaaggttg gcaggaaatc ttacgcatca
     7561 agtcaaaaaa gaaagaagct cagactcttc ctcctatcgt agaaaatgaa gaagcagaag
     7621 tagaactgaa aagtatggag aaagaaacac aacctccaaa accctatacc gaaggaacgt
     7681 tgatcacagc aatgaaaact gcgggaaaaa cgctggataa tgatgaagcg caagaaattc
     7741 tcaaagaagt agaaggaata gggacagaag ctacacgagc caatatcatt gaaaatctta
     7801 agcagaagca ttacatcgaa gtcaataaaa acgaaataac tgttacgcca aaaggaatca
     7861 ctctttgtaa ggcagtagcc aatgaaccat tactgacgag cgcagaaatg actgcgcgct
     7921 gggaagggta cttgagaaaa attggtaaac aagaggggac accatctgtt tttttagaga
     7981 atatcaaaaa attcatccta catttattgg cagaagttcc taatcaaatc gacaaggtag
     8041 acttttcaga tgagattagc gggacacgtc agataaaagc gatggagaag aaaaacgatc
     8101 aattaggaag atgtccaaaa tgtaaaaagg gaatagtcat gctttatcct aaaatcgcta
     8161 catgcttgaa tccggaatgc gacttcaaat tgtggccaac agtagctaaa aagaagctga
     8221 ctaaaacgaa tctcagagat ttgctttcaa aaggaaagac aagtaaagtt gtcaaaggtt
     8281 ttactggtaa aaaagggaaa ttcgatgcag ttttagtatt aaaagaagat atgacaatcg
     8341 gtttttcttt tccatggatc gaaaacagtg gaacaaaagc agttgatgga aacaaagaaa
     8401 catcaagaaa aaataatgaa aacgaagtag agtaaatgaa aacaatgaaa tagcggaata
     8461 tttattccga aaagtgagaa tataaatacc aataaaacta aaaaatatca gtgaatcaag
     8521 caaaaacgtt ggggtaggct gattttatat agatagcaag gagttttttt atttgaaaaa
     8581 agtgcattca tttttaaatt atcttatggg agcaatcacg attgtggctg tgtaccaagc
     8641 atttctaaag tatcctgatt acgggacgaa gttgttgtca ttgacgcaca atcaaacagc
     8701 atattcacca acgttgattt tatcgacttt gactgcagga gctaaaagcg ctacttattt
     8761 ttttcataaa acgacaagca tgctaaagcg aaagaaacag cgaaaacaat tgttggtggt
     8821 ggtagcggct atgatcatta tttattttct accagttatt acacaagtat ttcatcaata
     8881 ttttggttaa gaatacgaga gtttgtgaca aaagtcatgt cacaaacttt tttctttgtc
     8941 attttttcgt aagaaaaagc aaaataaaag tatatacatt gattaaaagt atatacttgg
     9001 atataatgta cttactatag aatgagaatg gtatagaggg tggcgaaata tgaaaaaaat
     9061 gaaaaagtat aaaattagaa aatctggcaa tagcgatgta acaactatcc caccagaagt
     9121 aaaagaattc atgggtgtgc agacaggtga cgcaatcagt tatgtttttc aatctgatgg
     9181 ttctgtacga atgatcaaag cacaagaaga acctgatatt gattcattag ttgattcgat
     9241 catgaatcaa tatgaagacg cgttaaaaga tttggttgat ttatgaggta tctttcgact
     9301 aaagaaatta tcaaaatcaa cgcgaaagtt attttacgtc actcgccagg cgaaatgatc
     9361 ggtgtaaaag atgcaaatgc attagatatg gccgtcaaac agccttcaca agcagtattc
     9421 aatcaagaac tctatcctga aatctatgaa aaagcggcga ttttagcaat taatttagct
     9481 aaaaaacatc cttttcataa tggcaataaa cgaactgctc ttgttgccat gttgttgttt
     9541 cttcaattga ataattgtcc agttgctttt tctcgtcaag aagctgttga ttttatcata
     9601 atgattacca ctagttcatt ggatttcgat tcactaaagt ccaagataac cagttattta
     9661 cgacaaaccg caataaaatg aacgaattaa aaactttatt tctcaattag atcagcatgt
     9721 cattaaggaa tatgctgatt tttttatgtt taatcataca aaatagtttt ttcataaaa
//
LOCUS       DAYYME010000102         9578 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000102 DAYYME010000000
VERSION     DAYYME010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.103"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..1457
                     /locus_tag="ACHBFW_002146"
     CDS             30..1457
                     /locus_tag="ACHBFW_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9318421.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1549..1914)
                     /locus_tag="ACHBFW_002147"
     CDS             complement(1549..1914)
                     /locus_tag="ACHBFW_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM9318422.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1911..2276)
                     /locus_tag="ACHBFW_002148"
     CDS             complement(1911..2276)
                     /locus_tag="ACHBFW_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM9318423.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2468..2716)
                     /locus_tag="ACHBFW_002149"
     CDS             complement(2468..2716)
                     /locus_tag="ACHBFW_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318424.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2715..2843
                     /locus_tag="ACHBFW_002150"
     CDS             2715..2843
                     /locus_tag="ACHBFW_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFM9318425.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2900..4594
                     /locus_tag="ACHBFW_002151"
     CDS             2900..4594
                     /locus_tag="ACHBFW_002151"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFM9318426.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4694..4867
                     /locus_tag="ACHBFW_002152"
     CDS             4694..4867
                     /locus_tag="ACHBFW_002152"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318427.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5245..6378
                     /locus_tag="ACHBFW_002153"
     CDS             5245..6378
                     /locus_tag="ACHBFW_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFM9318428.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6409..6729
                     /locus_tag="ACHBFW_002154"
     CDS             6409..6729
                     /locus_tag="ACHBFW_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFM9318429.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6758..7222)
                     /locus_tag="ACHBFW_002155"
     CDS             complement(6758..7222)
                     /locus_tag="ACHBFW_002155"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9318430.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7354..8607)
                     /locus_tag="ACHBFW_002156"
     CDS             complement(7354..8607)
                     /locus_tag="ACHBFW_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9318431.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     SPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8636..8953)
                     /locus_tag="ACHBFW_002157"
     CDS             complement(8636..8953)
                     /locus_tag="ACHBFW_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM9318432.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(8979..9299)
                     /locus_tag="ACHBFW_002158"
     CDS             complement(8979..9299)
                     /locus_tag="ACHBFW_002158"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9318433.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
BASE COUNT         3099 a         1880 c         1711 g         2888 t
ORIGIN      
        1 gcaaaaaaat acaaggcgga ggctctacta tgattgaaga atatattgag aaaaatattt
       61 taagacagct gtttttatgt gggcaattct atgtaaataa agaagtcaat ctagaaaagc
      121 tctccaatct cctgcatgtt tgcaaaacca ctttactcaa tgatatcaat aacataaaaa
      181 aagaatttga agaacagatc gcttatacac atcgggaaaa ggaccgttac actttatatt
      241 tttcagaaca tatacctcga tgcaagatca tgcagcaatt gtctcaaaat tcgttgtttt
      301 taaaaacttg tctgctttat ttggaagaag atgaacctga ttatcttcaa ttaactgaat
      361 gtgaattcat ctctgtcagc aaagcttatt cattaaaaaa acaagtatta gcttatttta
      421 atgactgcgg gatcgaaatc gaccgctact cccctcgatt cactgaaatg gaaagacgtc
      481 tgctgcttct taatgtgtcg tatcgtttag gcggttttaa ttcttgggag ctacctgaat
      541 cgttttttga acgtgccgat cgtttcattg aatccgtaac ggaaaatagt ggcagatttt
      601 atgataaaga aaataaagaa atcttatcga ttggttttgc tatcagtttc ctacgccagc
      661 aagtgtgtgc tgtgacaata gattcaaaat ttatcgaaga aatcaaaaaa cgtcctattt
      721 acaattatgt cgaatcagca tgggaaaaca ctgatttcca aacctattat aaaaaagaag
      781 aattcgcctt tattttgaca ctattcaatc tttgtaatta tggtttccat tcttatcagt
      841 tgattgcgga agactttcaa cagctacacc aagtgtttat tgacaatact cccgaaataa
      901 aagaactagt tgctaccttt gaatctcact tcaatcaaga attattcgga aaccaaccgt
      961 tcgaacgtgc gctgattcat ctgatgcgtt cggcttggga taactatcaa ttattcatgc
     1021 cagaaaaatt ttatctttta aatgaggaac agacaaattt atataaagaa gtccagacaa
     1081 ttttttccag ctggtccagc cagttacctt atgatctacg gttgaatcct aattgcatgc
     1141 gggcttttgt gatcgaattg agcggaattc tccgtctgac aaaagaacat ctgactatct
     1201 atatcgtcac aaattcggat gttcattatc tgatttatcg ggaagccttg gaagctgtaa
     1261 ctactttcga ttttcaagtt gcaccaacaa tttattcaag tatatctgat ataaagaagt
     1321 atgcgcagca gtcctcaaac cgggtattat gtgaacgtac attatataca ccagatgcgg
     1381 tccaatatga aaatattatt cctatttcga tcaatacgat cgatcgagct atcatttcag
     1441 ctgtgcaaaa caaataaggg gctgtgacaa aaatcatgtc acagcctctt atttgttgaa
     1501 gatgcccatt tctgtaacaa tctcttgata cacgtcaggc tcacaacctc acttattttg
     1561 gacatctacg ggtccgatct ctttatttaa aaaataagaa atgatggttc ggataactac
     1621 caacgccgct agttggaaaa tatcctgcaa ggtaggtttg ataatcgatt cgatgatccc
     1681 agctgaaaca agaatttcca aacttagaag tatatagcct cccaacatct ttttaatagc
     1741 attgttctcg ctgactcggt cgctttgcac ttttgtatgg acaaataaat atttcaaaaa
     1801 atccttgatt gatagacaaa caccccaaac caataaacag atagcaaaga tatcaagtcc
     1861 cagtacaagt ttttcgaaat atggaagaag aaactgcatc aactctgtcg tcatcctacc
     1921 tctcctcttc tttgtttttc tttgtcttct atttcctgat tcaagaaata agaaatgact
     1981 gttcgaatga taataataag tgcaagcttc aaaatatctg tccaagtcgg cttgataact
     2041 gactcgatga tatcagcaac gactagtact tccaagctta acaaaatata acttgctagc
     2101 agttttcttg tatctgcatt acgagtatta cgttctgcat agtttttctt gacatgagac
     2161 caatcaaaaa gtttgaatag aacaagaacg atcccgacaa ttaagatacc gatagaacaa
     2221 atatttaaaa tagcaatgat ccattctaga aattcaatta aatttgagcg aaacatatac
     2281 gctacctact ttcttttaat ttgttcctat tcttttattg taaaagaaag ttaccttgtt
     2341 cgtctattta aaagttgttc taatgataac ggttcgctta aatttttctt tttcactctc
     2401 ccatttttct ccctccatcc ataaatcgtg gaagtatcca aaacaacgta gcaagttaaa
     2461 ataaggctta gtccgtcatc agcttttcaa agaagcactg tctcggtgct ttttttgtac
     2521 ccaaatttct aaaaatagaa ccatacacct cctcaaatct tttcttctta cctaccctat
     2581 ttttatttac aggaaaatca gacacttctt cttttttgtc aaaatttcct ttttcttctc
     2641 gtcctatcat ttggcattct aataaaaacg aactatcctt ggatatagaa agagaaaaca
     2701 aggaggcgtt tttcatgagt aaaaaattaa tcaaagtcgg catcggttta ggtttgcttg
     2761 cccttggcgc tgcatatcta ggaaaaaaga caggactatt cgaagatgac agtcatttat
     2821 atgatgaatt cgaatcaatc tagcatccta tttagttgaa tcactacgga catacttgga
     2881 agacattgga ggaatcacaa tgagaaaaag taaagcaata atgattggtg ccggtattgc
     2941 caatatggca gcggctgttt atttgattca agaaggcaaa tggaaagggg atcagatcac
     3001 cttttattca ttggatgatc acggttcaaa tgatggtgca ccgaccgaca ctgtagtgga
     3061 tgaatattgg aataaaaacc atccattaga aaaccaaaaa ggctatattg ctcgaggcgg
     3121 tcgcatgctt aattaccgta cctatgtaga tttgatggat ctgttaagtc gaataccttc
     3181 tgttactgag ccagggatga ctgctgaaga agatacgaga gattttgatg caaaacaccg
     3241 aacctttgac aaagcacgtt tgatggaagg tggcaaagga atcattgatg gcggaaaact
     3301 gggattcaat aataaagacc gtattcttct gactaaactg attgctatgc ctgattcaga
     3361 agaagaaaaa ctagacaata tcactatcga agaatatttc aaagatgatc cacacttttt
     3421 tgaaacaaac ttctggttta tgtgggaaac aacatttgct ttcagaacac gtagttctgc
     3481 tcaagaattg cgtcgctata tgcatcaaat gatttatgaa ttcactcaaa tcgaacatct
     3541 agtgggcgtc aatcgaacac gttacaatca atacgaaagt attatgcttc ctttgatcga
     3601 gtacttgaaa gaacaaggat gcaaaatcat tttgaaccgc cgtgtcactg actgggaatt
     3661 caaagaaaca ccgatgcaag atgaaatcac tgtgactggt ttgaaaatta ccaatacact
     3721 gactcaagaa gaagaatacg ttgtggtcga tgaagataca gcagttattt tcactaatgg
     3781 gtccattaca gattcagcta ctttaggaga ttacgaaaca ccagctgtag aaaatatgga
     3841 ttatggtgca gcttctagtc tctggaaaaa agccagtgaa cgtttctaca acttgggaaa
     3901 tcctgacaaa ttctttgctg atcgagatgc aagcgaatgg gtcagcttta ccctgacgac
     3961 gaaaaaccat ttgttgttga atgaaatcac ccgtatcacg actcaagaac caggaaatgc
     4021 cttgaattca tttatttcaa ccacaccgat cacgccgtta ggccaaaaag atgtcaatat
     4081 gtctattgtc gtgcatcatc aaccgcattt tacgtctcag aaaccaaatg aaacagttat
     4141 ttggggttat ttcctttatc caagacgacg tggcgaattt gtggataaac agtatattaa
     4201 aatgaatgga aaagaaatgc tagaagaact gattggacaa ctttcaaaag tagatcctgg
     4261 accagtgaat attcgtgaaa aagaaacaga aattttcgac agcgtaataa ataatatccc
     4321 tgtctatatg ccttatgctt ccgcgttgtt caataatcgt gccaaaagcg atcgaccaaa
     4381 agtcattcct aaacattcga ccaatctggc ctttacagga gaatttgtcg agcagcctta
     4441 tcagatgatc tttactgaac aaagtgctgt acgttctgga gaaatcgctg ctttccattt
     4501 tgcgggtatt cctatgtccc gtcttgtcaa aacaccgcgt tacgacaaag atattcctac
     4561 tctagctcgt gccgcaaaaa aaatgtttga ataatcaaga ggttgtgaaa aagctgccct
     4621 gcatcaagta ataagaacaa ccaggctaaa acagcttctt atgtttttga tttggttgga
     4681 cttattaccg cagatgcaag cttttgaaca ccgtttacca agaggttatg aaaaagctgc
     4741 cctgcatcaa gtaataagag cagccaagct aaaacagctt cttatgtttt tgacttggtt
     4801 agacttatta ccgcagatgc aagcttttga acaccgttta ccaagaggtt atgaaaaagg
     4861 cgtttagttt cgagtatgaa gaaaaatttt gtgaaaatgg ttcctcacgt ttttgcataa
     4921 atttgactta ataccgagaa actgatccct tctgtcataa cctcttttta tttatgtctg
     4981 tttctttttg aaaacaacta ttttttcaac gtttactctc ttgccaatcc aacatcatct
     5041 aacggataca actatttcaa tgattaccat tacactaaaa aatcggaaac acacctctcc
     5101 gaacacaaat acataagact atagaatgtt tcatttaaga aaaaaagcgt ttcattatca
     5161 aaaaggtaag atctccttta aaatgaaatt atctgtagta ctaataatgc agacaatttt
     5221 atgaatctga aaggagcatc tccaatgaaa gcagtcactt ggcaaggaaa acagaaaatg
     5281 gaaatcagaa acgtagatga tccaaaaata ttagaaccaa cagatgcaat catccgtatc
     5341 acagcaacag ccatttgcgg ctccgatctg catctttacc atcatggtga agcagttttg
     5401 gaaaaagggt atgttgtagg acacgaacca atgggaatcg ttgaagaagt gggatctcaa
     5461 gttcaaacat tgaaaaaagg gcaacgtgtg gttatccctt ttaatattag ttgtggtcat
     5521 tgtcatttct gcctgaatca catggaaagc caatgtgatg aatcaaatcc agatcagtta
     5581 tatggcgggc ttttcggatt tggcaagttg aacggtaatc attggggtgg tcaagcggaa
     5641 tatctaagag ttccttatgc agactctact tcatttgttg taccagataa tgatcttcct
     5701 gatgaaaaag ttttatttct atcagatatt ttgcccacag cttattggag cgttgaaaac
     5761 gcaggagtaa aaaaaggcga tacagttgtg attctaggtt ctggtccagt cggtctattc
     5821 gctcaaaaat tcgctgttat ggctggagca gagcgcgtaa tcgcagtaga tccagtacag
     5881 caccgtctag ataaagcggc taattacaat ggggttgaaa cttatttctt agaagacacg
     5941 gcacaagctg gaacagaatt gtatgaactg gctaaaggtg gcgcagatgt cattatcgat
     6001 tgtgtaggaa tggacggatt agagcctgtc aaagaaaaag cgaaaaattt agtaagctta
     6061 caaagcggca caatctctcc attacagatg gcatcgcaag ctgtcaaaaa atttggaaca
     6121 gtccaagtaa ctggtgttta tatgactcca gcttcttctt acccgctgca agaatttttt
     6181 atgcgggata tcgctgtcaa gcatggacag gcaccaatta tccatctaat gccaaaaatt
     6241 tacgcaatga ttgcagaagg actattcgat cctacacaaa tcattactca ttcgctgcca
     6301 ctatcagaag cagcacaagc atatgagatc tttgacaaaa aagaagataa aaatataaaa
     6361 gttgttttga aaccttgaat tcagtaaaaa atacaggtga ttttctttat gcccaaaatc
     6421 aaacgaaata cccatgagat cgaaatagga aaagaagaag atatcgaaat acgaggacgt
     6481 cgcttccgct tctatttcca aaatcgttac acattgatct cactggctgt cgatttatta
     6541 accgggattt tctatatttt tggaagcctt gcttccttaa ctaaaatccc cgatcgatat
     6601 ggcacctatt tttatttggc tggtgctatt ttcttgacga ttcgtcctat tctcagaatc
     6661 gtaaggaaca tttttattta cgacgaaaaa aaagcaatga agagaacaag caaaaaaaaa
     6721 gaggcatgag aatttttcaa atctcatgcc tcttttttca ccctatcgta tcactttgat
     6781 acagacgagc ggtcgtccca ctttcttcct tgaccattgt caaaaatgtc cactcgtatc
     6841 gttcataaaa cgtggtatga tctgtaatta agtaaagttt ttcgtaccca agttgatgca
     6901 tttcttttct gatttccgct aatatccgct tggctatccc ctgcttacgc ttttccggtt
     6961 caacaaacag ggcacaaaga tttggtgtca gatcagggcg gttatggaag tcgttttcaa
     7021 tgacgcccgc tcctccgacg atggtttctt tttcatctaa gaccacatac cattgaggaa
     7081 tactttcctt attagcgatt ccagtacgaa tactttcctc atacacaaca gcaggaactc
     7141 cccattttga tgaaaaccaa ttcgcgcttt gttttactaa ttctgggtgt tctcttaatt
     7201 tgatccagtt atatgtaacc aatgtttaaa cctccgacta aaataaaacg tttacaaaaa
     7261 ttatacaagt ctttatgaaa attagcaatg acaacaatag tgatgtaaaa aaaaaaacgg
     7321 aaaacaatca cttgttctcc gttttttgat taatcaagca tctaaagctt cttttcttct
     7381 ttcttggatc gtagctacct tgacgaacgg tagatagatc aaaatagaaa gaaccaaatt
     7441 gacagcggct agtacaccac ctgcaatact ttttgttgcc aatacaccgc caataattgg
     7501 tggagttacc catggaggca taacggtagc tgctggcact aaacctgtag aagtcgcaaa
     7561 gtaagcaaca ctaactaaga ccattggtgt caaaataaat ggaatgaaca tgatcgggtt
     7621 caacacgata ggtaacccaa acatcgttgg ttcattaata ttgaagacac ctggcgcaat
     7681 cgacaagttt gttacaacca tatatggttt gtttttacgt cctactaaat aaatagctaa
     7741 cagtaaacct aaagttgcac ctgttcctcc taagttgaca aagctgtcaa agaaaggttt
     7801 gttcacgata aacggtgcgc tatgtccagc tgcgattgca ttaacgtttg cttcaatggc
     7861 tggcgcattg attgtttgca tcaaaggatc gaccatattc gctccgtgta aaccaaagaa
     7921 ccataagaat ggtgtgataa atgcaagcag taaggcagat ggataagagt ttgcaagtcc
     7981 cataaatggt tcttggacca ctgtatagaa agaacctacg atatcatcaa caccgaatgc
     8041 agcaaaaatt gcagcaacta aagcaaacgc agagatagtg atcattgctg gtagtaatga
     8101 agcaaatgct tttgcaactg ccggtgacac accatcaggc attttgatga tcaatttttc
     8161 atttccgctt aaacgtacaa agatttctgt tgaaataatc ccaacaataa tagcaataaa
     8221 caaaccgttt gagctcatac ctaatgctcc gcctaaagcg aagaaagagc ctaaagaaat
     8281 cacacctgcg gaaacaccgt cttttcggta gccgttagct aaatgataag caagcaagaa
     8341 agcaatcaag acagaaagaa tggcaaatgt accgttccaa gcagcagccc cgaatccttt
     8401 ccatgattca ccaccaaaaa ttgagttcat caattcttgg aacccgggaa tcggtaagtt
     8461 attgatcatt actgctaatg ctcctaagat cattaacggc atactcacca taaatgagtc
     8521 ccgaatcgca actaaatgcc gttgtgcacc aatttttgat gctacaggta taaaatgttt
     8581 ctccataaat gcaacaaatt tatccataaa tcttccctct tttctttact gtagattacg
     8641 cgttcaatcg acgatacaga tcaatgatct cttttgctaa atctgtaaaa gctatagatg
     8701 tcatcagatg atcttgactg tggaccgtca aaagcgtgac ttcaacgtga tttccttgtg
     8761 cttcttgtgt cagcatctct gtttgagagt gatgtgcttg gactaaagat tcctctgcat
     8821 ctttgatttt ttcatctgct aaagtgaaat cacctttttt cgcagcagca atcgcttcca
     8881 ttgcatcgct ttttgcattt ccgccaaaca tgatcagacc catgatggct tctagatttt
     8941 tttgatcttc catttattgt atcctccgat aatttttatt atttgtttat tagtttttct
     9001 gcttggtcga gtactttttc accattcatc atgccatagt cagccatatt gattacatct
     9061 aaaggaatgc ctttaggtgc tagcttttca gcaaattgat tttgcataaa gcgtacttgc
     9121 ggtcctaaaa gtaaaacgtc tacattttta gattctagtt gattatccgc atctgaagca
     9181 gatacagcaa agatgtctgc ttccattcct tttgcttctg ctgccttttg catttttgtg
     9241 actaggagac ttgtactcat tccagctgaa catacaagca tgattgtttt cttttccatt
     9301 ttaaacacgt tcctttctat acttacactt gtatataagc aagtagcgtg ccaactttaa
     9361 aagcgcttta aaaagttgat atcatcacat ttaaggtcta taccaagttg catacacact
     9421 aaaaaagaaa gcgcacacta aacacacaaa ttaattttta ttaccttttt tcttttaacg
     9481 tgcacaaaaa aagttgtgac acaaaacttg tcacaacttt tttcaaataa acggtattca
     9541 aaagcttgca tctgcggtaa taagtccaac caagttaa
//
LOCUS       DAYYME010000103         9524 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000103 DAYYME010000000
VERSION     DAYYME010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.104"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(81..1532)
                     /gene="cls"
                     /locus_tag="ACHBFW_002159"
     CDS             complement(81..1532)
                     /gene="cls"
                     /locus_tag="ACHBFW_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM9318434.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1873..4539
                     /locus_tag="ACHBFW_002160"
     CDS             1873..4539
                     /locus_tag="ACHBFW_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9318435.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFLTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4903..5655
                     /locus_tag="ACHBFW_002161"
     CDS             4903..5655
                     /locus_tag="ACHBFW_002161"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9318436.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5677..6831)
                     /locus_tag="ACHBFW_002162"
     CDS             complement(5677..6831)
                     /locus_tag="ACHBFW_002162"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFM9318437.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7016..9457
                     /locus_tag="ACHBFW_002163"
     CDS             7016..9457
                     /locus_tag="ACHBFW_002163"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFM9318438.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         3077 a         1711 c         2033 g         2703 t
ORIGIN      
        1 ttcggaagaa gggactgtga caagattttt gtcacagtcc ctttaatctt agtatatttt
       61 tctctacttg aataaaaaaa ttataggatt ggagaaaata atcgagaaat ttgttgcttg
      121 aataatagcc agttagacat ttccttgact gtttcagttg tcatggatga acaatgtgtc
      181 tcatcttctt tgaactgagt agcaagttct tctaaaaact caggatcgta aatgacagca
      241 cttgtttcaa aattcaatcg gtagctacga atatcttgat ttgctgaacc taccatgcag
      301 atttcatcat ccattatcaa tgtttttgca tggaggaagc cgttctcata gacaaggatt
      361 tgtacatttt ccttgatcag ctgccgagca taatattgtg ttgctcgata aataaaagga
      421 tgatccggtt tgttgggaat catgattttc acatctacac cagaggcagt tgcgattttt
      481 aaagcagcaa tgacactttc atcaggaaca aggtaaggtg tctgtatcca aacacgtttt
      541 ttagcagaag taatcaattt gataaatgaa agcttgattt gttccctttc gttattcggt
      601 ccgcttgaaa caagctggat ggatgtatca gcaagcttag attcatctcg atctgctttt
      661 ttaaagaaat aattcaaatg atagccgacc tttttttctt caggtaccga gacgttccag
      721 tctgttaaga agcgaagctg gagcaaagaa gaagcggcac cgaaaatccg tatatgcgta
      781 tcgcgccaat agccgaattt ttttgttgta tttacatatt gattggcaat attgaaacca
      841 ccggtataac taatctttcc atcgatcaca acgatttttc ggtgatcatg gtaattcaat
      901 cggaaacgaa gtaatgccct ttgtgaggta acaaatgtat ggacatgtcc accatttttg
      961 attagacgat tgaaatcttt tgcttttgtg ccatgagagc caaatgcatc atataatatt
     1021 cgaacttcca cgccttctgc ggctttttct tctaaaacat gtaagacttg ctggccgata
     1081 ttatctgtca caaatgcgta atattcgata tggatcgaat gtttggcttt ttttagatct
     1141 tgaagcagtg aatccaattt ctcttgtccg tctgtgagaa gagtgacaga attcattctt
     1201 gtcagcggca tacgatttaa tgaagagaag aagtcaacaa attgctgttt gtctttatcg
     1261 cccatgtcag gatcgtaatg ttcaatggta tctcctctaa aagactgaaa gttttctaat
     1321 tctttcaagt cactttgtcg gagatagaat tttttcttat ccgttaatcc acgtccgaaa
     1381 aataaatata atacaaagcc caccccagga agggcaaaca atactaagag ccatgcccaa
     1441 atggctgcta catctcgggg tttgatcaaa atagccacca aagcaataag tgcatttaat
     1501 agataaaggg cggttataat actagatacc acaaaaatgt tcctccttac atacaataac
     1561 attagtatag catgatagaa gaaagaagcc taccaaggtg aatatacttt tcactccttt
     1621 catgaaaatg taggtaaatc gggctaaagt agttgctttt tgtaggttaa taaggtagga
     1681 tattagtaca gttgaatatc tccttagggg agtaaccggc aacgtttgtt gcgataatat
     1741 caacagtaag ttaaccaaat ggttttctgg tattgtatga aatggtgaga cctaagacaa
     1801 atgcgtattt gtcttaggtc tctttttttt cagacaggac tagggccaag atcaaaaaat
     1861 gggggatgaa atttggaaaa caaggaacaa gcacaacaag cggtctataa agaaacgatc
     1921 gactcgttga tgcagcaaac gcagagctca accgatggat tgagcaccac gcaagcgaag
     1981 gaaaaattag aaagagatgg cttgaaccag ctaaaagaaa agccgaagaa gccaacttgg
     2041 aaattgttct tagaaacttt caaagatgca atggttatcg tattgctgat tgtcgcactg
     2101 attcagatgg tcatgggtga ctacatcgaa ttcttagtta tttttgcagt attgatgttg
     2161 aattctataa tcagtgttgt ccaaacaaaa aaagcagaag gttctttaga agcgttgaaa
     2221 aacctttcag cgcccgatgc tcgagtgatt cgtgatggta aggaaaaaac gattccggca
     2281 aacgaacttg tggtaggaga tatcgtgttg ttagacgcag gcgattatgt acctgctgat
     2341 ggacgtttac tagatgcggg atcattaaaa gtagatgaag gtatgctgac aggagagtcg
     2401 acttctgcag aaaaagaagt aacggatatc acaagtacgg taccaatcgg agatcgtaca
     2461 aatatggtat tcagtggaac gatcgtaact tacggacgcg gtaaattctt agtgacttca
     2521 actgggaata atacggagat cggacaagtt gctggccttt tggaatcaac aactgagcaa
     2581 gtcactccgt tacaacgagg tctggataaa ttcagtaaaa aactaagttt ggctattttg
     2641 gcattgtctt tattgatttt aggtatccaa ctgtttcgta tttatcttgg agaaggtaca
     2701 ggagatatga ctgcagatat cgtcagcgca gtgatgtttg ccgtagccgt agccgtagct
     2761 gctattccag aagcattaca gtctatcgta acaatcgtgt tatctttggg aacgaataaa
     2821 atggcaaaac gtcatgccat tattcggaag cttccagcag ttgaaacact tggttcgaca
     2881 agtatcatct gtacagataa gactggtaca ttgacacaaa acaaaatgac agttgttgac
     2941 tactttttag gtaatggcga tactggggat ttcacaaatg acccttcaaa atggacagct
     3001 gacgaacgtc gcttgatcga aatcagtgtt ttagcgaatg atgcatcgat cagtgaagac
     3061 ggtactaaat tgggcgaccc tacggaagta gcgtttatcg actttagtga aaaattaaac
     3121 cagccttatc aggaaatccg aaacaactac ccaagacaag cagaacttcc ttttgactca
     3181 gatcgaaaat taatgtctac tggacacaca attgataaca aaaattactt gtttgttaaa
     3241 ggcggaccag atatcgtctt ccaacgcagt aaaaaagtat tgctaaatgg tgaagtcgtt
     3301 ccgatgacgg atgaattgat gaaaaaattc caagagcaaa atgaagcttt tttaacacgc
     3361 gcattacgtg tactagcttt tgcttaccgt ccacttgata attttgattt atcatttgaa
     3421 gatgaagacg atttgatcct tgttggtttg cttgctatga tcgatcctcc acgtgctgaa
     3481 gtgactcagg cggtaacaga agcaagaagt gcaggaatca aaacaatcat gattactggt
     3541 gaccacaaaa caacagctgt tgctatcgca aaacaaatcg ggatttttga agaaggaaac
     3601 ctggctttga ccgggacgga actagatgaa ttgacagaag atcaactaaa cgaagtttta
     3661 gaaaaagtca ctgtttatgc acgtgtttca cctgagaata aaattcgtat cgttcgtgct
     3721 tggcaaaata aaggtcatat ctcagctatg acaggtgatg gtgtaaatga cgcaccagca
     3781 ttgaaacaag cagacatcgg aattgctatg ggaacgggta cagatgtggc aaaagatgca
     3841 gcagcgatgg tattgacgga tgataacttt gcttctattg tcagcgcagt agaagtagga
     3901 cgtaatgtgt ttgataatat caagaaagct atttcttatc tatttgcagg aaatttaggt
     3961 gcgatcattg cgatcatctt tgccttagtt gctgactggt ccaatccatt tacagcatta
     4021 caactgttgt tcatcaattt agtcaatgat tcactgccag caattgcatt aggtacagaa
     4081 aaagctgaac ctgctacgat gaaccgcccg ccaagagatc caaattcagg aatctttacg
     4141 gacaaaacat tgatttctgt aacgtatcgt ggtgtgctga ttgggattgc tgttatcttt
     4201 gctcaatggc ttggcatgca gcattcacct gaattcggtg tagccatgtc attttcaaca
     4261 ttgatttggt gtcggacatt gcaaacattc ccagcacgtt caaacacaca aacagctatc
     4321 caagcagggt tcttctctaa cagaagtgtc ttactagctg tagttgtatg tagcgcgctt
     4381 tactgcttaa caatgatccc aggactgaaa gaagtcttct ctatcccatc agcatttgga
     4441 ttgacagaag tctttacatg tatgggaatc gcacttgtga caattgtttt gatggaaatc
     4501 actaaattga tcattgtacg agtacaagga aataaataaa acgaatcaac gaggttgtga
     4561 gcctgccgtt taattctgag tcacaaggtg caatttcaac aaacagcttc tcatgtttgt
     4621 tgaatattgt gacttgtgac cgaagagttg gcaggagaac accgtttaag gaagaagttg
     4681 taaaatcagc attttgagct aatactgagg gtcatctgat tggacactgt ttattcggaa
     4741 agagggactt tgacacgact ttggtcacag tccctttttt tatttatgat agacggaaag
     4801 ccaatcattt tattattcat caaaagagat agaatagata aaagaggaga atgatttatt
     4861 ccgaaaagtg aaaacatata acaaaaaatt agaggaaatc aaatgaatga aaaagaatgg
     4921 gatgaatttg ctgaagaata ttatgaaatc cagcaagaat cgcaagtaac tattgctgaa
     4981 gatgtaaaaa attatctgaa aacggagaaa gtcctgcctg ctgaaacagc agcagacgtt
     5041 gctggaggat ctggcagatt tctttcctta gcaaaagaaa ttaatcgcta tgatttgatc
     5101 gatttttctt caggaatgct agaaaatgca aaaaaagaag cagaaaaaaa tgagttgcca
     5161 aatgtttcct ttatcaaaca gtcgttagaa gaatttatcc aatcaaatga gacatatgaa
     5221 atggttttta cagcagcaaa tccagcattg gtacatctta ttcaattaga aaaattgatg
     5281 aaaaagtcaa gcaaatggtg cgtgataatc agagtagtgg aatcaatcga tgatgttttt
     5341 tatcctatcg aaaagcaact aggaatgata gaagaagatc ctaatacatc gccagttgta
     5401 atggatcaat ttgaaacata cttaattgat gaaggattca aagtgaagaa aaaagatttt
     5461 acttatttta cacaggaatc aattactgaa gaattgatga agctatacta tgacgcgtac
     5521 gcaaaagatc ctgttgtgac caggtacatt cgcaaactat tcagtgaaaa acgagaaatc
     5581 cttagtacga ctaagctgac ctatagactg ctcctttggt ccgcagataa aaaaagaacc
     5641 tttcacgact cgtgaagggt tctttttatg attttattat attttgtagt aacgaatatc
     5701 attttcgatc tttgtaatcg aaaaaggtac atctgaagta tattctgcac attctccgtg
     5761 ttcaggaagc gtctcactga aaatcgtttc atattcgtca acagtgatcc gtgttcgaga
     5821 atctagcatc tcttgatgag attgtgcgaa gagatattct tgatagtttt cttccagata
     5881 acctgtaaaa aattcactta ctgcaccaga accgtagctg aatagaccga tgcgatctcc
     5941 tggctgtagt gatttcgaat tttccagtag agaagttagc cccaggtata atgaaccagt
     6001 gtaaagatta ccgattcgtc ggctgtaacg gatgctttct tcatagcgag ccataagacg
     6061 ttcctgattt tcttcgtctg tttggtctaa tacgctttgc aatgcctttt ttcccatctt
     6121 agtatacgga atatggaaag caatcgcctg ataatcttcg agtcctcgac ccgacaattc
     6181 tttatgtcga ttccaaactt tttggaatga ttcgatatac gtagaattag ataaaggacc
     6241 atcaacaaca ggaaattcgc tataatctgg acgccagaaa tcatagatat cttctgtcag
     6301 aaatacgctg tcgtcttcaa tcgataaaat acgcgggttt tgagtgatca tcatcgcaac
     6361 agcaccgaca ccttgcgtca cttcaccacc gcttgccaag ctgtaacgag caatatcact
     6421 tgctatgact agtacttttc gttctggatg atttttgaca tattctttcg ccatgtgcaa
     6481 agctgcggtg ccaccgtagc aagcctcttt tacttcaaaa gaacgagcaa aagattggat
     6541 tttcagtaaa tggtggataa ttaccgcact cgcttttgaa tgatcaatcc cggattctgt
     6601 tccgacgatg accatgtcga ttagctcacg gtctttttct gttacgattt tgctggcagc
     6661 atttgctcct agtgtaatga tgtcttcatt tgcacgattg actgccattt ggtcttgacc
     6721 aatccctata tggtattttg caggatcatc cccacggctt tctgccaact ctgtcatatc
     6781 taaatataaa ttaggaataa aaaaggaaag acgatcaatc cctattttca tttcaacacc
     6841 tctttactta tgataaaaaa cttactttac taaggtatca taaaaagatt aaaagaaata
     6901 taaaaaagag aatatttaag gattgttaaa atttaatctt agaaaaaaat aactggaaca
     6961 agagctattt gtttttacag caaagaatgg tatatttttg taggaggtgg cttacttgaa
     7021 agaagtcgtt atgatagatg ctgcaagaac gccgatagga aagtatcgcg gaagtttaag
     7081 tccatttact gcagttgaat taggcacact tgtgactaaa ggattattag ataaaacaaa
     7141 actgaaaaaa gataaaatcg atcaagtaat atttggaaat gtcctgcaag ctggtaatgg
     7201 tcaaaatgtt gccagacaga ttgctttgaa cagcggtctc cctgtagatg tcccagcaat
     7261 gacgatcaat gaagtttgcg gatctggtat gaaagcagtg atcttagcac gccagctgat
     7321 ccaattaggt gaagcagaac ttgtgatagc cggcggaaca gagagtatgt ctcaagcacc
     7381 tatgctgaaa cagtatcagt cagaaacaaa tgaatatggt gaaccaattt ccagtatggt
     7441 caacgacgga ttgactgacg cattttcaaa tgcacatatg ggattaaccg cagagaatgt
     7501 tgcaacacaa ttttctgtga gcagagaaga gcaggatcgc tatgccttgt cgtcccagtt
     7561 gaaagcagca catgctgtcg aagccggtgt attttctgag gagatcatcc cagtcaagat
     7621 ttctgatgaa gacgtgttat ctgaggatga agcagttcgt ggaaatagta cattggaaaa
     7681 actgggcacg ttacgtacag tattctcaga agaaggaact gtaacagcag gaaatgcttc
     7741 cccgttgaat gacggtgcct ctgtggtgat ccttgcatcc aaagaatacg cagaaaataa
     7801 taatctgcct tatttagcaa ccatcaaaga agtagcggag gtcggtattg acccaagtat
     7861 catgggaatc gctccaatca aagccattca aaaattgaca gatagatcag gaatgaattt
     7921 atcaacaatc gatctttttg agatcaatga agcatttgca gcttcttcta tcgtagttag
     7981 ccaagaactg cagttagatg aagaaaaggt caatatatat ggaggagcaa tcgcattagg
     8041 ccatccaatt ggtgcaagcg gtgcacgtat cttgactaca ttagcttacg gattgcttcg
     8101 tgaacagaag agatacggta ttgcttcatt atgtattggt ggaggtttag gcttggcagt
     8161 cttgttagaa gcgaatatgg agcaaacaca taaggatgtg caaaaaaaaa agttttatca
     8221 gcttacgcca tctgaacggc gcagtcaatt aatagaaaaa aatgtattga cacaagaaac
     8281 tgcgttgatt tttcaggaac aaacactttc tgaagagctc tccgatcaca tgatcgaaaa
     8341 ccaagtgagt gaagtggaaa tcccaatggg gatcgcgcag aattttcaaa tcaatggcaa
     8401 gaaaaaatgg attccgatgg caacagagga accatctgtc attgcagctg caagtaatgg
     8461 tgcaaaaatc tgtggaaata tctgcgcaga aacacctcag cggctgatgc gagggcaaat
     8521 cgttctatca ggaaaatcgg agtatcaagc agtgatcaat gcagttaatc atcgaaaaga
     8581 agagctgatt ctttgtgcaa atgaaagtta tccttcaatc gtgaaacgcg ggggcggtgt
     8641 tcaagatatc tctactcggg aattcatggg gagtttccat gcatacttat ctattgattt
     8701 tttagtggat gtaaaagatg caatgggcgc taacatgatc aactcgattc ttgagagtgt
     8761 ggcaaacaag ttacgggagt ggttccctga agaagagatt ctattcagta ttttaagtaa
     8821 ttttgctaca gaatcattgg cttctgcctg ttgtgaaatc ccattcgaac gcttagggag
     8881 aaacaaagaa atcggtgaac aaattgccaa aaaaatccaa caggcagggg aatatgcgaa
     8941 gcttgaccct taccgtgcag caacgcacaa caaagggatc atgaatggta tagaagctgt
     9001 agtggcagct acaggaaatg atacacgtgc ggttagcgct tcgatccacg cctacgcggc
     9061 aagaaatgga ttataccaag gattaacaga ctggcagatc aaaggggata aactagttgg
     9121 aaaactaacg gtgcctttgg ctgttgccac agtaggcgga gcttccaata tcctgccaaa
     9181 agcaaaagca tctttagcta tgttagatat tgattcagcc aaggaactag cacaagtaat
     9241 tgctgcagta gggttagctc aaaacctggc tgctttgcgt gcattagtta cagaaggaat
     9301 ccaaaaaggc cacatgggac ttcaagctcg ttcattagcg atctcaattg gggctattgg
     9361 tgaggaaatc gagcaagttg cgaaaaaatt acgtgaagca gaaaagatga accagcagac
     9421 agctatacaa atattggaaa aaatccggga aaaataaaaa agcaatatct ttagaagaat
     9481 aaagcaagat ttacagttat tttatttact gcagatcttg cttt
//
LOCUS       DAYYME010000104         9306 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000104 DAYYME010000000
VERSION     DAYYME010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.105"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            113..307
                     /locus_tag="ACHBFW_002164"
     CDS             113..307
                     /locus_tag="ACHBFW_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318439.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            576..875
                     /locus_tag="ACHBFW_002165"
     CDS             576..875
                     /locus_tag="ACHBFW_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318440.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(972..1406)
                     /locus_tag="ACHBFW_002166"
     CDS             complement(972..1406)
                     /locus_tag="ACHBFW_002166"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9318441.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1638..2348)
                     /locus_tag="ACHBFW_002167"
     CDS             complement(1638..2348)
                     /locus_tag="ACHBFW_002167"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM9318442.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2338..3198)
                     /locus_tag="ACHBFW_002168"
     CDS             complement(2338..3198)
                     /locus_tag="ACHBFW_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFM9318443.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3203..3841)
                     /locus_tag="ACHBFW_002169"
     CDS             complement(3203..3841)
                     /locus_tag="ACHBFW_002169"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFM9318444.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3856..4155)
                     /locus_tag="ACHBFW_002170"
     CDS             complement(3856..4155)
                     /locus_tag="ACHBFW_002170"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9318445.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4198..5682)
                     /locus_tag="ACHBFW_002171"
     CDS             complement(4198..5682)
                     /locus_tag="ACHBFW_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM9318446.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5702..6163)
                     /locus_tag="ACHBFW_002172"
     CDS             complement(5702..6163)
                     /locus_tag="ACHBFW_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9318447.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6181..7248)
                     /gene="ulaG"
                     /locus_tag="ACHBFW_002173"
     CDS             complement(6181..7248)
                     /gene="ulaG"
                     /locus_tag="ACHBFW_002173"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFM9318448.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7466..8236
                     /locus_tag="ACHBFW_002174"
     CDS             7466..8236
                     /locus_tag="ACHBFW_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9318449.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8509..>9306)
                     /locus_tag="ACHBFW_002175"
     CDS             complement(8509..>9306)
                     /locus_tag="ACHBFW_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9318450.1"
                     /translation="EYRKKKGLTQVQLAEGICEQATVSNIENKNMSPGINILSALCKK
                     LNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKVSNSDLLNQIL
                     FYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLSKGELDFAKVY
                     VERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEYNKKHKSTYAL
                     EYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2884 a         1910 c         1473 g         3039 t
ORIGIN      
        1 aaaaactttc aaattgattc tttttattta tcataaagat atagatacat aatcattttc
       61 acttacatat atttgaatat taactaaaaa cagcaagaaa gaagaggatc aaatgtatta
      121 tgtagaagta aaaacaaaag gcgtcaaaaa caaacaatat gtgaaaggta tcagtaacga
      181 atatccttta cttggttcat ggaaagaagc tgctcctttt tccaaaccat gtgccataaa
      241 aataaagaac gagctagaaa aagaactgac ttgtggtaaa gctgttgtag atatcatcga
      301 aaagtaaatc caatgtatag aaaggaagat cacacatgaa aagcgacgta caaattgcta
      361 agattataga attagaagaa gaagacgcag tgttgacaat tgaaaaaaaa taaatgtttg
      421 tataccatat gattatttca atatgtatcc tggaattgga gatctcgtag agctatatca
      481 agatgaatca aatatacttg tcattcatca tgaaatatag agacttataa aattgtttat
      541 ttttataatg tatcagtata gaaaggaaat aaaacatgga ttatactgtt gaatttgaca
      601 gtgaactact taagcaattt ccacaagaag aacatttcat gtataaagaa gcaatttccc
      661 acaaagtgac agagtatttg gagcataatt tttttcgtat aaagccagtg cgtactcgtt
      721 cggcaaaagg aatatacgag atgaaagtca aaattaataa aaaaaattat cgcatagcat
      781 ttgctttaaa aaaaaatcat gtgttcattt tttatattag ttcgaatttg caaaaaattg
      841 tatttgataa agaggtgagt aaaaaaataa aatagctgag cattggagga tagattgagt
      901 gatactaagt ctatcctctt gtttttctaa agaactaagc tagaattttt tcttttgtac
      961 gttattatat gttatttgtc attaccaaaa agcacctttt ttcttctagt atctatgaca
     1021 taagaaatac cacccatttc ctttacttga acaaatccta ctgattcata aaattttttt
     1081 gttttctcag tatcatctgt caataattcg atttggactg gtccataact agtgatcttc
     1141 tccaaagctt cattcaaaag cactttgcct attccttggc gctgatacct cggatgaatg
     1201 aggatatctt gaataaatgc gatcgtcgca aaatcagtga tccaacgaac gattccgata
     1261 atcttccctt ccccattacg ctttatcaga gattcagatt gttcaaatgc tctagctaac
     1321 cttgcagtgt cttttgtgta attcgtccaa ccaacgctct tatataattc agaaatctca
     1381 tcgatcataa gggaatcaat attcattttt ttcttccttt cttatacaat tctccctcta
     1441 ataatacagt tctttgacta tttaatctat cacatataac tctcctgtaa agattctctc
     1501 gattccttaa aattcctctt tccttttaga atgcatactt tttgataaat aaaaaggttg
     1561 tgataaattt tcttcacaac ctctgtttct ctctatcaag tgttatcttt taattttcta
     1621 cccttaccct agcatctcta ttgcccgtag tatgcatact ttccatgttt gcgaaaataa
     1681 tgtttatcta gtaaatattg cgggatttca catatattgg ggttgatcag ctcgttttct
     1741 ttggccatca gacaaatttc atctaagatc aaactgtttt ctactgcttt cataggtgaa
     1801 tccccccatg tgaacggtcc atgtccatag acaagtactc cagggacttc gtttggatct
     1861 aattttcttt ccttaaacgt ctcgacaatc acattccctg tatgaacttc gtaagcttct
     1921 cttacttctt ctttcgttaa ttggcgtgta caaggaacct taccataaaa agcatcagca
     1981 tgagtcgtgc cataagctgg caaatcgcgt cctgcttgcg cccacatgac ggagtgcgtg
     2041 gaatgagtat gagtaatagc agttatatct tcaaaagttt gatacaaaac aacatgtgtg
     2101 gggagatctg aagatggttt taagctatct tcttctatta tatttcccga caaatctgtt
     2161 accaccattt gatctgcttg catgcattca tatctgacac cgcttggctt aatgacgatg
     2221 actcctagct cacggtccac ttcactcaca ttgccccaag tcaatttgac taatccgtat
     2281 cgcggcaatg ccagatttgc ttcaaggaca cgttgcttca ttgctgcaat tttttcttca
     2341 caagacatca tagccagcct ccttcaattt agaacttaag tagtcttttg cctgctggat
     2401 ttctcttcga aagtcttggc ttttttcact ccacatttca atcagaaatg ttccatcata
     2461 atctagacgt ttcaaatgtc ttaataatcc aagaaaatca acacaaccgc taccaaaagg
     2521 aacatcacga aactggcctg aatattcttt tgttacaggg taagtatctt ttaagtggat
     2581 agcagtgata taatcgatcc ctttctcaag ttctcgagcc ggattatttt ccggccatgc
     2641 ggacaaattg cctaggtctg gatatacttg tagaaaaggt gaatgaatct gttctttgat
     2701 ttctaaaaat tttgaaatgg agttcataaa tggatcatcc atgatttcca cggaaagaat
     2761 gatgccatat ttagaagctt cctttacgct ttcttttatt ccttcgataa agtcttctct
     2821 ggaattcatt gatttctctt cataataaac atcgtatcca gctatttgga ttactttgac
     2881 gcttagatta tgcgccaaat ggattgcttt tttcattagt tgtttcgctg cttttttctt
     2941 ttctggatca gcagaaccaa acggaaatcg ccgatgcgca cttaaacaaa tcgaatggat
     3001 acgtacaccg gagctgaaca tctcttctct cagctcagct atcttttctt cagaccaatc
     3061 taatcgtgct aaccgctcat ccgtctcatc aatcgacatc tcgataaaat cgaagtccat
     3121 ttcttttaca agggaaaaac gttctttcca tgaaatatct ttgggtaacg ctttttcata
     3181 aatcccaatt tgagccattt ccttaccccc agatacgaat gatctcatct ttgaagtttt
     3241 gtgcggcctt catcggatca tttgctgctg taataccacg accagtgata aaggtataca
     3301 catcgatccc ttcaaataag tgtaatgtgt gtggatttaa tccaccagtt acagaaacat
     3361 taaagcctag ttcaatcagt tttttgatct tcgataaatc tttttcaccc caagtttcac
     3421 cagataacag cgcatcacgg ctttgatggt agattacctg ccggataccg atgttatgcc
     3481 aatccatggc ttgttcaaaa gtccaattgc cgtagagttc tacttgcaat tcacctattt
     3541 ctttttgtgc tgcctccata gttggaatcg ttgcacaaca aatgactgtc atccagtctg
     3601 ctcctgcttt tgcaacatta ctcgccactg ttcctccagc gtctgcacat ttcgtatcag
     3661 cgactagttt tttatctggg aacatactgc gaatacagcg aatcgctttt tgtccttcct
     3721 gtaagcaaag aattgtgccc acttcaataa tatctactac atcaccaact ttcataacat
     3781 ctcctaatgc atgttctaag tcattgtgat ctaacgcaat ttgcaaattc ggcctgctca
     3841 tcttagttcc ttcctttact tgtttttttg gtaccacttt gtttcagcaa atttttcact
     3901 gatttctttg actgacatca catttttgat accgataact gtaactcctt ttttctctgc
     3961 ctgagtaaac atattcaaaa aattgactgg cgtaaaaact acatcgtatt ggccagctga
     4021 actttttccc tctgaaatgg cacaatgatg gattttggta attggtacgt ttagttcttt
     4081 cattgctttt tccacacttc tcatcatcat caagcttgtt cctgatccat ttgcacacga
     4141 tactaaaatt ctcatcttaa tccctccatt atgagtgttg tctaatcaga ttttctttta
     4201 tactctctgc ttttctgcat atgtgtcata gtcttctaca atcaaaaaat aaccatcagg
     4261 atttttccga tattgtaact gcggaatagc caataaacca atgacaacta cggcaattcc
     4321 agcataacct aggactttca tgattactgt aaacaatggc caagctgttg cccagtcgaa
     4381 catcccgata taaccaccgt atgttgacat cccaatccat gtagcgaata atgctgaacc
     4441 gcctacttgt atgattcctg aaagaaacgg gaacaggcaa gcggctttga atccacctcg
     4501 gttattggca taaactgcta taacggcatt atcaaagaac aacggaatga aaccggctat
     4561 gacgatcgtt ggtgatttca ataaaatcag taaaccaatc atcaaaaact gtcccaaagc
     4621 accaaacaag aacccaatcg ttaccgcatt tggcgaacca aagccaaaag tagcagctac
     4681 gtcaattcct ggaacagctc ctggtaaaat cgtgtttgag attccttgga aagattgagt
     4741 tagttcatcg acgaatgtcc gaacacctaa ttgcaagatc gccagatata ccgcaaaatt
     4801 taaagaagtc gtcataatgt agaataagaa actctgccct tcttgcatga attgcgcttg
     4861 aatcaaataa tctttgccta gtacaatcag aatgatccca aagaaaaata gcatgagtaa
     4921 cgaagtagca accatattct cattgaaaat cgacaagaat cctggtaatt tgatatcttc
     4981 aatttttttg tcttgcttga ttgatctatt tttcttttca tcccgctttt tcatccactc
     5041 agctaatttt gcaaatataa agattccgaa catttgctga tgtgccactg caaatccggc
     5101 accttctgtc aattcctgcg tgattcctac tgttaagtta gaagctactg tccaataaca
     5161 acctaaaatc agtcccataa aaatcaatgc ttgtatccta cctagatcag gaaagcaaaa
     5221 cagtaaaatc caaaaagcgg tagctgcttg ttgaacctga acgtttccgg tagtgaaaac
     5281 ggaacgcagt ttcgtatatt tctgaaaacg gactaataag atatttaaaa caaatgcaat
     5341 cagtaataaa atcatcacat ctccaaaggt acggccaaat gtctccatta atccagcatc
     5401 cacagcattt tgtccaaaat aaggatctgt aaccatggca tccaaattga accgttcctt
     5461 tagtccaacc aagattggcc gaaaattatt gaccaaacca ccagagccaa cagtcaaaat
     5521 aaagtaaccg accgttgcct ttaagaatcc agcgatgcat tcatgaagcg ggcgtttcag
     5581 caaaatatag cctaataaca cgataaaacc aatcaggtat gctggctgcg tcaagatatt
     5641 tgttgcaaaa tacgtccaaa tgcttaacaa aaattccccc atcttttttc tcctctatca
     5701 attaattata ttctgccatc acaacttggt aatcatcgat ggagttcgta gagatgagac
     5761 gctcaaccaa tccatctgtc attaataatt cggataattt ttggatattt tctagatgtt
     5821 cttctggatc ttttgctgcg agtgtaaaaa acaaactagc cgttttctcc tcattctccc
     5881 cttcactttc aaaacggatt ggctgtttca acttggtaaa tgaaatagct gttccaaaaa
     5941 caccatcact tttttctgaa gaatgcggca ttgctacttg aggtgcgatc acaatataag
     6001 gaccgtattt ttctacacaa tggatgatct cctccacata cgtttcatca ataatttcct
     6061 tatcgatcaa tacaagacag ctttcggcga tagcttcacg ccaatccgtg atttctttat
     6121 ctgggtaatg aatcaattga tggtcaaaaa agtatttcag caagaaactc cccttctttc
     6181 ttataaaaac gacggaaacg gtgtatcatt ttcaatcgag aagacatctt caaatcctcg
     6241 ataaaaatta aactcaagat catctttgtt tgtcggatag atgaatttcc cgcctacttg
     6301 ccagatataa ggtttaaatc catacttcag gcgatccttt ttgaatttcc aaatttcagt
     6361 gatctctttt ggatcagcca taaaattaga ccaaatatcg tagtggacag gaatcactac
     6421 ttttgcattc aatgactccg ccatgcgcag catatcaaca gaagtaactt tgtctgttat
     6481 ccctcgtggg ttttcaccat aggcccctaa acagacatcg attttgtgtt cattaccatg
     6541 tttggcaaat aaattggagt aatgtgaatc tcctgcatga taaagattcc ctcctgatgt
     6601 ttcgaataaa tagtttactg caatttgatc catctcctga ggcatttttc cttttaatac
     6661 ctcttctgga tcttcacaag tgaccaacgc tgtccgatca aacgcttcta aagctactac
     6721 attgatgtct ttgattttga ctgcatcacc tggctttact gtaatcgttt tttcttcagg
     6781 aattccccat ttttgccacg tgcgaaccac ttcttttggc ccaatgaatg ttgcttcagg
     6841 acaattttga tgaactgctg cggctgtatg gatatctaga tgatcggaat ggatatgtgt
     6901 aacgattaaa gcgtctacat tttttatcgc aaaaggatcg attacgaaag gctgagtacg
     6961 taaattaggc tgcatatttt gaacgccact catacgcatc atctgatggc cttttttcat
     7021 catgccattc ccatgactac gttttcccgt cccacaccat agatcacata aaatattcgt
     7081 tccttcatga cttttcagcc aaattcccgt acagcccagc caccacatag caaaagttcc
     7141 aggatccact acttctgttt cgatttcttc attcaagtat gtgccccact caggaaatgt
     7201 ttctactaac cacttttctt ttgttacttc attgacagtt ttactcattt gcttgctccc
     7261 cccatatcgt tgttccaact tacaagtaaa tattaatgtg tatcagcatc catgaaaacc
     7321 cttttatatg accgtttgtt ttttatatga ttttctttca gtaatcaatg attgtttcag
     7381 tataataaag acagatatga atgtttaaat gaacaactag tcaaataaat ccatggatca
     7441 agatgtccct caggaggcaa aagtcatgaa aaactcactt gtcaatattg aaaaacgtca
     7501 aaaagaaatt ttggaactgc ttcagcagac tgaacaacta tcaactttcg aattagctga
     7561 atcccttgat gtatctatta gtacgatacg aagagattta aatttattag aagaaaaaaa
     7621 cgagatcata cgcaattacg gttattgttc ttataattac aaaaaccaga ctgattttga
     7681 tcaatctggt cctgaaagga ttaaacaagc gattgccaga gaagctagta aatacattaa
     7741 cgattatgat acgctgttta tcaattctag ctcgactgcg ttgaaaacac tggattttct
     7801 tcaatctaac catttgacag tcattacaaa caacgtgaaa atcaattact ctccgcacaa
     7861 agataattgt agttatattt taactggtgg agagatgcgc tttccaaaag aagcactagt
     7921 gggcgacatc gcattaaata cgatttcttc agtcaatgca gacatttgta tcattggctg
     7981 tagcggagtc gatgtagtac atggggtcac aacaaagata ttaaatgaat caacgatcaa
     8041 cgaaatgatg gtcaacaaga caatcaaatg taagatttta gtcgctgatc atcgaaaaat
     8101 cggactgaca tcaaagttca agattgctga tatttcagtc tttgattatt tgatcactga
     8161 taaattttgt tctgaaaaag tattagagga gatcagaaat acggggataa aaaccattca
     8221 agtagattca ttttaagaac caggacgtaa atatgtaaat aggattatca aaaaaatgaa
     8281 taacaaattt atcgataaat gaaaaacacg ctacgctcaa attgataaag gaatcccaag
     8341 atgtatccat ccaatcgata ccatcataat ctattgcttc ttttataacc gttatatgtt
     8401 gcctaaatag gaatcaaaca atatcgaaaa aaggaggtat gacaaaagtc acacctcctt
     8461 ttttcgaata aacggtattc aaatcagaac actgatttca caaccacttt atttcaggtc
     8521 gttttcaatc acctttaccg cgtaatcatt tccgttgaaa cgcgctattt tttttgcgtc
     8581 catatatctt tctaaataat catcttctcc taattgctta tggcaagaag caatttcata
     8641 aagcaagtat tccaatgcat aagtcgattt atgtttctta ttatactcaa tacccttctc
     8701 acataattgg attgctttat cataatcctc gattttacgg taataggtgg cgatatcata
     8761 atatacaatg cagaccatca gattatcaaa atcagtctca ctcaatattt taagtgtacg
     8821 ctccacataa actttcgcaa aatctaattc tccttttgac aagtagatca ttgccacgtt
     8881 tgcagctgct tgtaagtgat ggatatctgt ttcggtcgtt tgttccaaca cttgattaaa
     8941 ataaaataat gctgcatctg gttttttcgc ataaagatga tcaagcagtc cttgataaaa
     9001 taaaatttga tttaaaagat cagaattact tactttttcc tgatcgatcg tttgtaaaat
     9061 tttataagat tcttggtatt ttgatacaga attcaatgat tgaatctcat ttattttttt
     9121 cctgattttg tcttcttctg tttctactaa tatatcggag atctcgacat tcagcttttt
     9181 gcaaagcgca cttaagatat tgatgcctgg cgacatattc ttgttttcga tattgctgac
     9241 agttgcttgt tcacaaatgc cttctgctaa ttgtacttgt gttaacccct ttttctttcg
     9301 gtattc
//
LOCUS       DAYYME010000105         9274 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000105 DAYYME010000000
VERSION     DAYYME010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.106"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            36..1451
                     /locus_tag="ACHBFW_002176"
     CDS             36..1451
                     /locus_tag="ACHBFW_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM9318451.1"
                     /translation="MFLLVFLPVKVQGAEKDEIYERIKQSGELVVGLSADYAPYEFHA
                     NVDGKDKIVGFDVSIAEKIAKDMGVKLKVEELGFDALLGALKTGKIDLVISGMAVTEE
                     RLQEVDFSDPYFVVQQKVLVRKEDKNKFKTTADFSGLAVGAQKQTTQEELVKNELTGA
                     ELTSLQKVPDLINNLLNEKVEGVVLEQPVAEAYAQQNKKVALTPVKFAQGEKTTAIAM
                     SKNTPELKNHVNASIKTINDDGLLKDYQKQANQLMFQDDQSFLQKYGPYYLIGTKYTI
                     LLAFVGVLFGMVFGSLLALMKLGKNKFLKALAIIYIEYVRGTPLLVQIFIVYFGTGVL
                     GLDLSKLVAGCIAVSLNSAAYVAEIVRAGINAVNKGQMEAARSLGMDQKEAMRFIIFP
                     QAIKNILPALGNEFVTIIKESSVVSVIGVSELIFQTGVVQGASFKPFLPYVLVSLIYF
                     VLTFSISRILALVERRMGLNA"
     gene            1444..2166
                     /locus_tag="ACHBFW_002177"
     CDS             1444..2166
                     /locus_tag="ACHBFW_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9318452.1"
                     /translation="MLNVKKLSKSFGENEVLKSIDLSVKEGEVVVIIGPSGSGKSTIL
                     RCMNLLEEPTSGEIFFKGQNITAPDSDINQIRQKMGMVFQSFNLFPHMNVLENLTITP
                     VKIKKEDPKKAKEHALSLLNQVGLKEKVDSYPTSLSGGQQQRVAIARALAMNPDVMLF
                     DEPTSALDPEMVGEVLAVMQDLAKKGMTMVVVTHEMGFAKEVADRVIFMADGIIQEEG
                     TPKEIFENPQNSRTQDFLNKVL"
     gene            2186..3337
                     /locus_tag="ACHBFW_002178"
     CDS             2186..3337
                     /locus_tag="ACHBFW_002178"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM9318453.1"
                     /translation="MDKSKIARRFQQPAENLLMNIAILAKEKEDIIDLSIGDPDLITD
                     PTIIEAAYTDVKNGATNYTAPDGSKEFLSTVVDFYQKKYGLSVECNQVRATVGAMHGM
                     YLVLQALLDPEDEVIIHEPYFSPYREQVILSGGKPVFVPTYEEKGFQLDVEDLKAAIT
                     DRTKVIVINSPNNPTGAVFSESTLKKIAEVAIENELYIISDEIYEDFAFKEKFVPMAR
                     FAPDNTITVSGVSKGFAMTGWRLGYMIAPAYINQIAGLINENITYSAPTASQRGAIYA
                     LKHYEQLVPPVVEVFKERLAYIEKRVAEIPYLSLSSVSGSMYAFINISKSGLDSVTFV
                     EEVLEKSGVLFVPGKAFGETIGDNYIRLAATQPLEILGEAFDRLAKLNF"
     gene            complement(3685..3960)
                     /locus_tag="ACHBFW_002179"
     CDS             complement(3685..3960)
                     /locus_tag="ACHBFW_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFM9318454.1"
                     /translation="MNKIKMNVQGRVQSVGFRYMTKIVADQLGITGTVKNEDDGSVTI
                     EAAGDDEAIKKFVEEVKRSPSPSGRVQYVDLQENPMMEDRKKFVVIG"
     gene            4068..4832
                     /locus_tag="ACHBFW_002180"
     CDS             4068..4832
                     /locus_tag="ACHBFW_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFM9318455.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKGEYLLEGFHLIEEAVAS
                     NAKVNELFITQRGLTEWASWLEENQALSVTLVSEEVMATVSDLPTPQGIMAVVEAEKR
                     EIPNFEGGWLLLDNVQDPGNVGTMIRTADAFGLSCVVLGKGTADVYSTKVLRSMQGSN
                     YHLPIIQEDLTKLLPILKEQGFITYGTELNEEAVSLNDIQTTENYAIVMGNEGQGVRP
                     EVLALTDKNVYIPIQGNAESLNVGVAAGIMLYHFSV"
     gene            4956..5459
                     /locus_tag="ACHBFW_002181"
     CDS             4956..5459
                     /locus_tag="ACHBFW_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM9318456.1"
                     /translation="MTEKWREDEEYMSYINDLLETDAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYKLAKKWGGDARATARAGLLHDLFYYDWRTTKFDEGTHAYVHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMTASMKQKWQRY
                     FGKEQSI"
     gene            5724..6371
                     /locus_tag="ACHBFW_002182"
     CDS             5724..6371
                     /locus_tag="ACHBFW_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFM9318457.1"
                     /translation="MEKTIKPYQYYFIKREVEQLLSAYSSVNDPKTVQTVQALAAEKI
                     HEVLGHPNTAVNEFMEKVLDVTLKKEKAERLLEELKVIVLPFIQPTKPQIDKLFRKVK
                     KLKQPDWNQLDLREHTYIGWNDPGSQKKFMIYYEEEKLKGISGTLSPTINKGICSICQ
                     RTSNVSLFLSTTKTGSDGTYTKKGNYICHDSDQCNQQLVQLAPFYEFTERVKRER"
     regulatory      6414..6481
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            6532..7800
                     /locus_tag="ACHBFW_002183"
     CDS             6532..7800
                     /locus_tag="ACHBFW_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFM9318458.1"
                     /translation="MENRLPDPLKVIIYMIKWLLITAILGIFMGSLSAFFLTSLTDVT
                     AIRLEHPWLLFLLPISGGLFAYLYTTYGGNSSRGNNLVIDQGNGGNEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALAENFARWLKLNKIEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEALFPSFFAALFANFVTESFNVKHTHYQMGAIPAWDPS
                     LFFKLLLAGIAFGIIGWLFSRSIVWLKAWYAKRIPHPVLRNFIGGAVVVLVVVILQTQ
                     RYLGLSLPLLQDAFNGSAHPYDFLGKLGFTVLSLGAGYQGGEVTPLFEIGATLGATLA
                     PILHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGGQAASYFFMICLISFMCSGN
                     SGIYSAQKVEVKKGILFLPILGRISRQKEK"
     gene            complement(7977..9179)
                     /locus_tag="ACHBFW_002184"
     CDS             complement(7977..9179)
                     /locus_tag="ACHBFW_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM9318459.1"
                     /translation="MNLTRDLIFLVIYILGMSGILYWRKRQSDPALFFYCGTWTFMMM
                     VTYFVVLEVFYHTFYFLIPLLFFSIFAFFYFKEKRRLINGVLFNTFLIVFGGYLGWLL
                     IETQNIFIGGLLALIGIPILILLAIGIYILIIFLFWNGVVVMKKESRSLANLLTLLLG
                     VALTIFLILDYTLVHWLPQWANALFAVIPFIFAYLLIVFFNFMTVSILYQFNRPRYNQ
                     DYIVVLGAGLIDGERVSPLLAKRINKAIAFYWAQSKATLNPPVLLMSGGKGTDEKVAE
                     AIAMKQYALEKGIPERDILVETNSTTTLENMLFSKEIMDQQMNGPYRAIFTSNNYHIF
                     RAGLYARQAKLNANGIGAKTALYYLPNAILREFIAILVLHKKRHLIICGLMIGFAVFS
                     ALILKFAQ"
BASE COUNT         2963 a         1612 c         1998 g         2701 t
ORIGIN      
        1 tgaaaaaagg aataaacata ttattagtgg cgctgatgtt tctactggtt tttttaccag
       61 taaaagtaca aggtgcagaa aaagatgaaa tttacgagcg gataaaacaa agtggagaat
      121 tagtcgttgg cctatctgca gactatgcgc cctatgaatt ccatgcgaat gtggatggta
      181 aggataaaat cgttggtttt gatgtatcca ttgccgaaaa gatcgcaaag gatatgggtg
      241 tgaaactaaa agtagaagaa cttggttttg atgcattatt aggtgcgttg aaaactggca
      301 agatcgattt agttatatct ggtatggcgg tgactgaaga acgtttacaa gaagttgact
      361 tttctgaccc atatttcgtg gtacagcaaa aagtactcgt tcgtaaagag gacaaaaata
      421 aatttaagac gacagccgac ttttctggct tagccgttgg ggcgcaaaag caaaccacac
      481 aagaggagtt agtgaaaaat gaactgactg gtgcagagtt gacctccctg caaaaagttc
      541 ctgatctgat caataatttg ctgaatgaaa aagttgaagg tgttgtgctg gaacaaccag
      601 ttgcagaagc atatgcccaa cagaataaga aagtagcttt gactccagta aaatttgcgc
      661 aaggcgaaaa aaccacagct attgcgatgt caaaaaatac accagaactg aaaaatcatg
      721 tcaatgcgtc aatcaaaacg atcaatgacg acggcttgct gaaagattat caaaaacaag
      781 cgaatcaatt gatgttccaa gatgatcagt cctttcttca aaaatacggt ccgtattatt
      841 tgattggaac aaaatacact attttattag cttttgtggg tgtattgttt ggaatggtct
      901 ttggtagttt gcttgcactg atgaaattgg gtaagaataa atttttaaag gcattagcca
      961 ttatttatat cgaatacgta cggggaacac ctctcttagt tcaaatcttt atcgtttact
     1021 tcggaacagg agtactggga ttggatctat cgaaactggt agcaggttgt attgctgtct
     1081 cgttgaatag tgctgcttat gtagccgaaa ttgtccgtgc cgggatcaat gcggtcaaca
     1141 aaggacagat ggaagctgca cgttctttag gaatggacca gaaagaagcc atgcgtttca
     1201 tcatcttccc acaagcaatc aaaaatattc ttccagctct aggaaatgag tttgtcacga
     1261 tcatcaaaga gtcctcagtt gtttcggtca ttggggtatc ggaattgatc tttcaaacgg
     1321 gcgtggtaca aggtgctagc ttcaaaccat tcctaccata tgtgttggtc tctttgattt
     1381 atttcgtgtt gaccttcagt atttcccgta tattagcttt agttgaaagg agaatgggtc
     1441 tgaatgctta atgtaaaaaa actgtcaaaa agttttggcg agaatgaagt cttaaaatca
     1501 attgatcttt ctgtgaaaga aggagaagta gtggtgatca ttggcccttc aggaagtggg
     1561 aaaagtacaa ttctacgttg tatgaatctg ctggaagaac caacttctgg tgaaatcttt
     1621 tttaagggac aaaacattac agcccctgac agcgatatca atcaaattcg tcaaaaaatg
     1681 ggaatggtat ttcaaagttt taatttgttt ccccatatga atgtactcga aaatttgacc
     1741 atcacacctg taaaaatcaa aaaagaagac cctaaaaaag caaaagaaca tgctctatca
     1801 ttattgaacc aagttggtct aaaggaaaaa gtggacagtt atcctaccag tttatctggg
     1861 ggacagcaac aacgggtagc aattgctcga gcattagcaa tgaatcctga tgtgatgctt
     1921 ttcgatgaac ctacatctgc acttgatcct gaaatggtcg gtgaagtgtt ggcagtcatg
     1981 caggatttag ctaaaaaagg gatgacgatg gtcgtggtca ctcatgaaat gggttttgct
     2041 aaagaagttg ccgatcgtgt gatttttatg gctgacggca tcattcaaga agagggaaca
     2101 ccgaaagaga tttttgaaaa tccgcaaaat agtcgaacac aggatttctt gaacaaagtg
     2161 ttgtaacaaa gatggaggaa gtaagatgga caaaagtaag attgcacgcc ggtttcagca
     2221 accagcagaa aatctgttga tgaatattgc tattttggca aaggaaaaag aggatattat
     2281 cgatttatcg attggtgatc ctgacttgat cactgatccg acgattattg aagcagctta
     2341 tacagatgta aaaaatggtg ctactaatta tactgcgcct gatggcagca aagaattctt
     2401 gtcaacggtc gtagattttt atcaaaagaa atatggattg agcgttgaat gcaaccaagt
     2461 tcgagctaca gtcggagcaa tgcacgggat gtatctagtt ttacaggcat tactagatcc
     2521 agaggatgag gtcatcatcc atgaacctta tttttcacca tatcgggaac aagtcatttt
     2581 atctggaggc aagcctgttt ttgttcctac ctatgaagaa aagggcttcc agttggatgt
     2641 ggaagattta aaagcagcga ttacggatcg tacaaaggtg atcgtcatca attcgccgaa
     2701 taatcctacg ggagctgttt tttctgaaag cacgttgaaa aaaatcgctg aagtcgcaat
     2761 cgaaaatgaa ctctatatca tttccgatga gatttatgaa gactttgcct ttaaagagaa
     2821 gttcgtaccg atggcgcgat ttgcgcccga taatacgatc actgtcagtg gtgtatcgaa
     2881 ggggtttgcg atgactggct ggcgcttagg gtacatgatc gcaccagcat acatcaatca
     2941 aattgctggg ttgatcaatg aaaacatcac ttattccgca ccgactgctt ctcaaagagg
     3001 tgcaatctac gccttaaagc actatgaaca gcttgtaccg ccagttgttg aagtattcaa
     3061 agaacgatta gcttatattg agaaaagagt ggcagaaatc ccttacttat ctttatcttc
     3121 tgtttctgga agcatgtatg cttttattaa tatcagtaag agtgggctcg attcagtaac
     3181 gtttgtggaa gaggttttag aaaaaagcgg cgtacttttt gtccctggaa aagcgtttgg
     3241 ggaaacgatc ggggacaact atattcgact agcagcgact cagccattgg aaatcttagg
     3301 tgaagccttt gatcgtttag caaaactaaa tttttagata aaactgtata tttgaaaaca
     3361 gctatgggta tccattagaa caaagaggga aaatgatcct ttcgagagtt ggcttgttga
     3421 ctgggcagcg tcaggatttg acaatgaaaa agtggtagtg gaaaatcaaa gtcgattttc
     3481 cactaccata aaatattata gactccattt tattgaacca aaatttttga agtcctcccg
     3541 cttttacgta tgcctatcac tggttactaa actcgctaat aagaatgttt ttttgtcgat
     3601 ctaggatgga gagaggggca aactttttcg atctacatac agacagtccg atttttctga
     3661 catttcctat tcatttctag aaggtcagcc tattacaaca aacttttttc tgtcctccat
     3721 catcggattt tcctgaaggt ctacatattg gacacgtccg ctggggcttg gtgatcgttt
     3781 gacttcttcc acgaattttt tgattgcctc atcgtcgccc gcagcttcga tcgtgacaga
     3841 gccgtcatct tcatttttca ctgttccggt gatccctaat tgatccgcta cgatttttgt
     3901 catatagcga aaaccaacgc tttgtacacg tccttgcacg ttcatcttga ttttgttcat
     3961 tttattcacc gttctttctt ttatttttaa attaatgata ccgacttttt tgctactgaa
     4021 caagtgcaat gatctaatcc atggtataat cagttgaggt gaaaaatgtg aaagagatcc
     4081 aatcagcaaa aaatccaaga atcaaagaat taaaaaaatt acataagaaa aaatatcgtg
     4141 aagaaaaagg ggaatatctg cttgaagggt ttcacctgat cgaggaggct gttgcctcaa
     4201 atgcgaaggt caacgaactt tttattacgc aacgcggtct gactgagtgg gctagttggc
     4261 ttgaagaaaa tcaggcgctt tctgttacac tagtatcaga agaagtgatg gcaactgttt
     4321 cagatctacc aactccgcaa gggatcatgg cagtggtgga agctgaaaaa agagagatac
     4381 cgaattttga aggtggttgg ttgttgctcg acaacgtaca agatcccggc aatgtaggga
     4441 caatgatccg cacagccgat gcttttggtc tatcttgtgt tgtactaggt aagggaacgg
     4501 cggatgtcta cagtaccaaa gtattaagaa gcatgcaggg gagtaactat catttaccga
     4561 tcatccaaga ggatctaaca aaactattgc ctatccttaa agagcaagga tttataacct
     4621 atggaacaga actgaatgaa gaagcagtgt ccctgaatga tatccagaca acagaaaatt
     4681 atgcgatcgt gatggggaat gaaggtcaag gcgtacgtcc agaagtgctg gcgctaacgg
     4741 ataaaaatgt gtatataccg attcaaggaa atgcggagtc tttaaatgta ggagttgcag
     4801 cgggcatcat gctttatcac ttctccgtat agttaaggaa ctattaagct ttctgcattt
     4861 tgcgaaaacc tcactagaat attacgaaca ctagtggtat actaagttca atttcaaaga
     4921 taaatggaat tcaaaaaggt ggcagatcga taattatgac agaaaaatgg cgtgaagacg
     4981 aagaatatat gtcttatata aatgatctat tagaaactga tgcagtaaag aaattagcaa
     5041 attacacgca acatgtccac tcgacacgat tagagcattc aatcagtgtg tcttactata
     5101 gctacaaatt agcgaaaaaa tggggcggtg atgcaagagc gactgctcga gcaggccttt
     5161 tacatgattt attttactac gattggcgta cgacgaagtt cgatgaaggt acacatgcgt
     5221 atgttcaccc aaggatcgct gtgaaaaatg ctgaaaaaat cacggagctt tcagatctag
     5281 agcgtgatat tattttgaaa catatgtggg gagcaacgat tgcccctcct aaatataaag
     5341 aaggttatat cgttactttt gtggataaat attgtgcagt caaagaagca gctcaaccaa
     5401 tgacagcttc aatgaaacaa aaatggcaac gatatttcgg taaagaacaa tcgatttaag
     5461 gtagttaact aagtatttga cccgagtatt ttgaaaaaaa tcctcgggtt ttttgtttag
     5521 acattgacta attggtcttt ttagtgttta ctttgttgag atgaaaaagt acataaccaa
     5581 cataaaaata aagggggtga tgataagatg cgatgtgtat cttatttaaa agagtttgag
     5641 ggctttatct aaaggcactt tgatagaaag atcacttttt tcttcaaagt agccgatttt
     5701 gaaagaagaa aggactcaaa gtcatggaaa aaacaatcaa accatatcaa tattatttta
     5761 tcaagcgaga agtcgagcag ctgttaagcg cctatagttc agtgaatgat ccaaagacag
     5821 tccaaacagt ccaagcatta gctgcggaaa agatccacga ggtacttggt catcctaata
     5881 cagctgtaaa tgaatttatg gaaaaggtac tagacgtcac tctaaaaaaa gaaaaagcag
     5941 aacgactatt agaggaattg aaagtgatcg ttcttccatt catacaaccg acaaaaccgc
     6001 agatcgataa attattccga aaagtgaaaa agttaaaaca accagattgg aatcaactgg
     6061 atttaaggga acatacttat attggttgga atgatccagg tagccaaaaa aaatttatga
     6121 tctattatga agaagaaaaa ctaaaaggga tcagtggaac attatctcct acaatcaaca
     6181 aaggaatttg ttcgatttgt caaagaactt caaatgtttc actctttctt tctaccacaa
     6241 aaactggttc agatggaaca tacacgaaaa aaggaaatta tatttgtcat gatagtgacc
     6301 aatgtaatca gcagttggta caattagctc ctttttacga attcacagaa cgagtgaaac
     6361 gtgagcggtg atttcattgt atttttctga aaaatatgct atcttatttg gtgaagtgaa
     6421 taggtgatgg tgttcgcctt taaacgttga tcgtaagata actaatgacg cctactaaag
     6481 aaacggggct accccttttt ttagtaggcg tttttttgag gaggaaatga gatggaaaat
     6541 cgattacctg atccattgaa ggtgatcatc tatatgatca aatggttatt gatcacagca
     6601 attttaggta tttttatggg aagtttatcc gcattctttc taactagttt gactgatgtg
     6661 acagctattc ggctggagca tccttggtta ctttttcttt tgccgataag tgggggactt
     6721 tttgcttatc tatatacgac gtatgggggc aattcttccc ggggaaacaa tttagtgatc
     6781 gatcaaggga atggaggaaa tgaaaagatt ccattacggc tgatcccact gaccttattt
     6841 ggcaccatta ccacgcatct ttttggtggt tctgttggtc gggagggtac agcggtccaa
     6901 atgggcgggg cacttgctga gaattttgct cgttggctaa aattgaacaa aatcgagcgt
     6961 gagattcttg tcatcagtgg gatcagcgca ggttttagct cagtatttgg gacacccttg
     7021 gctgggacat tgtttggatt agaagtattg gcaatcggaa aagtgagaac cgaagcactg
     7081 tttccaagct tttttgcagc actttttgcg aactttgtaa cggaaagttt caatgtcaag
     7141 catacccatt atcaaatggg ggcgattcct gcatgggacc cttcactttt cttcaaattg
     7201 ttgcttgcgg gcattgcatt tggtatcatt ggctggctct ttagccgttc gatcgtctgg
     7261 ttaaaagctt ggtatgcaaa gcggatcccc catccagttt tacgcaactt tattggagga
     7321 gcagtcgtgg tactcgtagt agtgatttta cagacgcaaa gatatctcgg attaagctta
     7381 cctttattac aagatgcatt taatggaagt gctcatcctt atgattttct tgggaaactt
     7441 ggctttaccg ttctatcact tggcgcggga taccaaggag gcgaagtgac ccctttgttt
     7501 gagattgggg caactttagg tgcgacatta gcacccattc ttcatgtatc agtacctttt
     7561 ttagcaggat tagggtttat tggggtgttc tctggagcaa cgaatacacc gattgcttgc
     7621 tttgttatgg ggatcgaact ttttggaggt caagcagcca gctacttctt tatgatctgt
     7681 ttgattagct ttatgtgttc aggaaatagt ggaatctatt ctgcgcaaaa agttgaagtg
     7741 aaaaaaggaa tcttgttcct accgatcctt ggaagaatct cacgacaaaa agagaaataa
     7801 aaagaggttg ggacagaagt gtttaactcc gagaaataag aagaaattca cgaaaattgc
     7861 tttttaaatt tttgtgaatt tcgcttattt ccgaaggagt tgcttctgct cccaccgttt
     7921 attcggagtt agagtctgtg gcaaaactga tttttagttt tgtcccacgc tcttttttat
     7981 tgagcaaatt tgagaataag agcagaaaat actgcaaagc caatcattaa gccacagatg
     8041 ataagatggc gttttttgtg taagacaagg atggcaataa attctcttaa gatagcattc
     8101 ggtaagtagt atagtgcagt ttttgcaccg attccgtttg cattgagttt cgcttgtcgc
     8161 gcatatagtc cagctctaaa gatatggtaa ttatttgaag taaaaatcgc gcgataaggc
     8221 ccattcattt gctgatccat tatttctttt gaaaacagca tattttctag cgtcgttgtg
     8281 gaattcgttt ctactaaaat atcacgctcg ggaatccctt tttctaaagc atattgtttc
     8341 attgcaatgg cttcagctac tttttcatcg gttccttttc ctcctgacat gagcaagact
     8401 ggcggattca aagttgcttt actttgtgcc caataaaatg cgatcgcctt attgatcctt
     8461 ttagcaagta ggggagatac gcgttcacca tcaatcaagc cagcgccgag aacaacgata
     8521 taatcttggt tataccttgg acggttgaac tgatacaaaa ttgaaacagt cataaaatta
     8581 aagaaaacga tgagtagata agcaaaaata aacggaatga cagcaaatag agcatttgcc
     8641 cattgtggga gccaatggac taatgtatag tccaatatca aaaagatagt caaagcgact
     8701 cctaacaaga gagtcaaaag gttggccaaa gaacgggatt cttttttcat gactacaaca
     8761 ccattccaaa ataaaaaaat gatcaaaatg tagataccga ttgccaaaag aattaatatt
     8821 ggtatcccga tcaaagccaa taacccaccg ataaagatat tttgcgtctc tattagtagc
     8881 caccctagat aaccaccaaa gacaatcaaa aacgtattaa aaagtacgcc attgattaac
     8941 ctacgttttt ccttaaaata gaaaaatgca aaaatactga aaaatagcaa tggaatcaaa
     9001 aaataaaatg tatgataaaa aacttctaaa acgacgaagt aagtgaccat catcataaaa
     9061 gtccacgtcc cacaataaaa gaatagagcg ggatcgcttt ggcgcttgcg ccaatagagg
     9121 atgcctgaca ttcctaaaat atagataact aaaaatatca ggtctctagt aagattcatt
     9181 gaatgaactc ctttcgattt gtaaattttt tcttaaaaaa gatagcatca tttagtagta
     9241 aatatatcga atatagaaaa gttagttact aaat
//
LOCUS       DAYYME010000106         9263 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000106 DAYYME010000000
VERSION     DAYYME010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.107"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..662)
                     /locus_tag="ACHBFW_002185"
     CDS             complement(<1..662)
                     /locus_tag="ACHBFW_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9318460.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QL"
     gene            796..1083
                     /locus_tag="ACHBFW_002186"
     CDS             796..1083
                     /locus_tag="ACHBFW_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318461.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1499..3037
                     /locus_tag="ACHBFW_002187"
     CDS             1499..3037
                     /locus_tag="ACHBFW_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFM9318462.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3034..3999
                     /locus_tag="ACHBFW_002188"
     CDS             3034..3999
                     /locus_tag="ACHBFW_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9318463.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3986..5308
                     /locus_tag="ACHBFW_002189"
     CDS             3986..5308
                     /locus_tag="ACHBFW_002189"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM9318464.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5371..5475
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5549..6292
                     /locus_tag="ACHBFW_002190"
     CDS             5549..6292
                     /locus_tag="ACHBFW_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9318465.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6307..7299
                     /locus_tag="ACHBFW_002191"
     CDS             6307..7299
                     /locus_tag="ACHBFW_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9318466.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7311..8048
                     /locus_tag="ACHBFW_002192"
     CDS             7311..8048
                     /locus_tag="ACHBFW_002192"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9318467.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8038..8760
                     /locus_tag="ACHBFW_002193"
     CDS             8038..8760
                     /locus_tag="ACHBFW_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM9318468.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8844..>9263)
                     /locus_tag="ACHBFW_002194"
     CDS             complement(8844..>9263)
                     /locus_tag="ACHBFW_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM9318469.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3028 a         1601 c         1959 g         2675 t
ORIGIN      
        1 tctaactgtt caagaggatc aagtggttca aatgtttctg tagtctgttt tgcttccaat
       61 tggggcaaaa cagatccaat ttccagatac ttcactttat tcaatgtata agatagaata
      121 gaacagctaa gtaatgtata cagaaaatgt gtgagatcca aataaagcaa ggagacaact
      181 aaaataccaa aagtaatcaa gaaaagactt ttgccaatct taatatgaaa atatttttca
      241 atggcttgat aaatgatatc tgttccgcct gtgcttccgc cgaatcgaaa gacgagaccg
      301 caaccaatgc cagaaagaat acctgtcatc aaactattga tcagtagact atctacagca
      361 aaatgtatat gaaggctctc ccataaatgt aaaaagaaaa ctagcgcaaa tgttccgacg
      421 atcgtccgga gaaaagcgtg ttttccaaaa agaaacaatg agataagcaa taaaggtaaa
      481 ttaaaaagca gtgaagtgac agaaggagcg aatccaaaaa gattatgcaa aagcaaagca
      541 ataccagtaa ccccaccatc tgccagctta gatggaatat tgattcctgt cacagctaat
      601 ccgtagattc ctgcgcctaa aatgattccg cacaattctg taaataaatt taattttttc
      661 atttctagcc tcttttcttt tgaaaatttt cagcaacttc tttattatac tgacttatac
      721 tcttacttca agaggaaaat gaaaaaaaat tatttttggc tgtttctttt tatttatcaa
      781 ataaggagtg gaaaaatgcg agcagcagac ttattgacaa ttcttacaaa atcacccaaa
      841 aaaacagagt atcaaggagt ttttttggct caagaacaaa aactggtgcc tgtgacaatg
      901 gtccagataa ataaagcaaa tcaatttatc tttttatttg aacacggcaa agcaccactt
      961 gctatgaaag acatctttct ttttttgatg aagaaccgtg agaaagctgt ttattatcag
     1021 tcaaaagaag aactcattcc tctttatgga gtgaaagaga tagaaaataa agtggtgata
     1081 taagtaaaaa taagaaaaca agacgtttat gatgtcaaaa caaccatttt ttacattttt
     1141 ggcaaaaaag tgtcatgata cagtataata ttcgttgaac aggccaataa aggtgttctc
     1201 ccccacgaga tttctacttt attggctgtt tctttttatt aaaataaaag ttgtgtgtgt
     1261 aaaaaagata tagctgattc caagtgacaa tttcgtatag atttttatgg tattcgtagt
     1321 gaaaaaacga gaaccaaaaa gttgatccga ctttgttgct tggattcttt ttttgtcagc
     1381 atatttagag gtattttttt cttttttttg ttcgtgctgt aaggttaagg caagacaagt
     1441 tagttgggaa aaaggaaaga aatgtttata cttaaagaat aacagaaagg aaggtcgcat
     1501 gaataatttt atacaaacat taagtgaaag aaaaggcgag ttgctggaag ctgcatttca
     1561 acatctgtcc atttctctca tttctttgct gattgcagca ctgattgcta ttcctttagc
     1621 gatttgggca gccaatcata aaaaaatggc agaagtctta ttgcagatca cgggtgtgct
     1681 ccaaacgatt ccttcacttg ccttattagg tttacttatc ccatttgtag ggatcggaac
     1741 tgttccagca ttgattgcac ttgttattta cgctttgctt ccgattttcc aaaacacgta
     1801 tattggtcta gctgaaattg acccgtctat tgaagaagca gcggtagcat ttggtatgtc
     1861 cagaatgaga cgcttgctga aagtagagct tcccatcgca ttacctgtca ttatctcagg
     1921 tatccgaact gccttagttt taatcatcgg tacggctact ttggcagcat tgatcggagc
     1981 aggtggatta gggacattta ttttgttagg tattgaccga aatacacctg ttttgacatt
     2041 gattggtgcg atcagttctg ccttgcttgc aattatattc agcggactga ttcgctggtt
     2101 gcagcataag aaacctcgat attccatcat tactttagca gcagtggtgt gtttatttgg
     2161 cggcgtttct ttatatcagt ctgccttgtt taaaaatgaa accatcacga ttgctggaaa
     2221 attaggagcg gaacctgaaa tcctgatcca aatgtataaa gaattgattg aagacaacac
     2281 aaaaacagaa gttgaattaa aaccgaattt tggcaagacg agttttttgt tcaatgcact
     2341 ggatcaaggt caaatcgata tctatcctga gttcacaggt actgtcctag aaagcttggt
     2401 gaaggtacct gaagcaatga aaagtgctga tttgacaaaa gaaaaaacat atgacgaagc
     2461 agaagaatta ttgaataagc aattcgatat gacattatta aaaccaatgg cttaccaaaa
     2521 tacgtatgcc ttggcagtca agagatcttt tgcgaacgaa catcaactta aaaagatttc
     2581 cgatttggca gctatctctg atcagatcaa agcgggattc acactggaat ttattgatcg
     2641 ttcagatgga tataaaggaa tccaagaagc atatggcttg aatttttcaa gtgtacaaag
     2701 catggagcca agtttgcgtt accaagcaat caataatgga gatgtcaata ttgtggatgc
     2761 ctattccaca gatagccagc tcaaggagta cgatctagtg actctagaag atgatcgagg
     2821 gttattccca gcttatcaag gcgcaccgct gatgaaaaaa gattttgcta aagaacatcc
     2881 tgaaatcgtt aaagcactga ataaattaag cggaaaaata acagaggatc aaatgattga
     2941 tatgaattac caagtcaatg tcgaaaagaa aaagccgaca gaagtagcac gaatcttcct
     3001 gaaaaatgaa ggattagtaa aggaggaaga tcagtgacta aattaatcga gttcaaacat
     3061 gtgcaaaaaa agtatgatgg aaagtatgtc atcgatgacc taaatctggc aatcaaaaaa
     3121 ggagaaatct ttgttttggt tggtccttcc ggtagtggaa aaacaactac tttgaaaatg
     3181 atcaatggat tatctaaacc atcagcagga gacatctact tcaaaggaaa atcgttaaat
     3241 gaatataatc tgcagaaaat gcggtggaat atgggatatg tgctgcagca aatcgctttg
     3301 ttcccgacca tgacagtcaa gcaaaatatt gaagtcatcc ctgaaatgtt aggttgggaa
     3361 aagcagaaaa gagcagaccg tgtagatgaa cttcttcaaa aagttggctt atctcctgat
     3421 atttatcgag atcgtatgcc tagagagttg tctggaggag aacagcaacg gatcgggatc
     3481 atccgagcta ttgctgcaag cccagatgtg attttgatgg acgagccatt tagcgcatta
     3541 gatccgattt caagaaactc attacaagaa ttggttttgt ccttacacga agaattgggc
     3601 acgaccatcg ttttcgttac gcacaatatg gaagaagcaa tcaagctagg tgatcgtatt
     3661 gcatttatga aggatggaga aattatccaa tgtgacacac cagagcagtt attgatgaat
     3721 cctaaaaacg actacgttcg gcatttcttt gatgaaccga aacagacgaa agagtggcga
     3781 gtagaagatt tagtcgtcaa tggatatttc ttaaatgaaa taccagaaaa tgctcgtcaa
     3841 caagtttgct ttgatacgcc tatgaaagaa gtatattcgc ttctttcggt gtatccaagc
     3901 attgtgatag tagaaaagca aagaagtatt gggtcgctga caagtaaaga aatctttgct
     3961 tttttaagtc gagaggagga aggaaatgaa aacatatgat gcaatcatca tcggttcagg
     4021 tgtgagcggc ttaagtgctg cttatggatt gaaagaagca ggtaaaacag tactagtggt
     4081 cgaagaagat ctatggggcg ggacttgtcc taaccgtgga tgtgatccta aaaaagtctt
     4141 gttaagtgca gtagaggcac gtaatcgggt aaaacaattg agtggaaaag gttttaacga
     4201 gatcccaact gccaactggg aagaacttca aaaattcaaa cgaacattta cggatccagt
     4261 cccagaaagc cgaaaaaaac aattagcaga agctgaaatc gatcatcttt caggaaccgc
     4321 tcgttttttg gatgattcat ctattgaagt caacgaagaa gtcttccatg cggattactt
     4381 agttttggca accgggcaaa gacctacgat cttaccagtg gagggaaagg aatacttgaa
     4441 aactagtgca gactttttat cattgcctgt actgcctaaa gaaatcattt ttatcggtgg
     4501 tggttatatt gcttttgagc tagctacgat tgccaatgct gcagggagca aagtaaccat
     4561 tgtccatcat aatcaacggc cattaaaaga atttgaggcg tctttggtag aggaagccgt
     4621 ccatcaaatg gaagcatctg gcattcagtt tgcttttggt gttgaaaccc aaaagatcat
     4681 ttcagaaggt actcgctatc gtttagtcgg aaaagagacg gaattagtag cagatatgat
     4741 tttttgtgcg actgggagac agccgaatac agaatcttta gcacttgagc aagcgaatat
     4801 cgtctttgat aaacacggaa ttgcagtcaa tgattatctg caaacgagca atccaaaaat
     4861 ttttgcgtgt ggagatattg tttcaaggaa aaccccaaaa ttgacaccag tcgccacttt
     4921 tgaaggaaat tatgtagcta agcggataac ggatgcaaca agcgaaccta tcaaatatcc
     4981 gattatacct acgatcgtct acgccagtcc taaacttgct gaagtaggag ttacaaaaag
     5041 tcacgcctct tcgtcagatc aagtggtaga gatggatctt accagttggt tcacctatca
     5101 tcgtgtcaat gaaccagttg ctaaggctga attgaccttt gaccagcaaa attatttgat
     5161 tggtgctgca gtgattagcg agcaagcaga cgaattgatc gatgatttga cactcgtcat
     5221 caatcaaaaa ttgactaaaa aagagttgga tagctatatc atggggtatc cgacattggc
     5281 aagtgatctc tcctatttat tgaaataatc agcatgataa ctaaagcgct tgttgctttt
     5341 tccttcttgt gctacacttt aaaaaatgaa tacctgtttc tggggatgtc gtagacgaca
     5401 tgagagtaag ttgtgaactt agacccattg aacctgtcag ttaatactgg tgaagggaga
     5461 aacatagcag acgcttgaag cgggttacta tgtcgtgccc atttcaagcg ttttttgtta
     5521 ttcatattag aagaaaggag gtaacaaaat gaagaaaaat ctttatccag cagtcagtat
     5581 ggcattcatt ttaattattt ggcagattgt tgtcatagtt tggaaaatac ctgcctttat
     5641 tttacccgga cctgctgctg tattttctgc attagcggca gatgcacaag tattactttc
     5701 ccattctgtc acgacattaa atgaagcagt gcttggtttg gtaatagcta gcgttctttc
     5761 ttttattact gccttggcaa tggattattg gaagttttta gaaatgtctt tttatccgtt
     5821 actagtgatt tcacaaatgt tgccaatcat ggtgttaggg ccattactta ccctatggtt
     5881 tggtttcggt atgcttccaa aagtgatcct tgttgtcttg ataagctatt ttccgatcgt
     5941 agtttctttt tcggatacat tgaggaaagt atcaaaagag caaatcatct ttttgaaaac
     6001 gatgggggca gatacaggga agatttatcg aatatataaa ataccagcgg gtttatccgg
     6061 cttcttttcc ggtttaaaag tggctgctac ttattgtgtc agcggagcag tcgttggaga
     6121 atggctaagc gctcaagcag gcttagggta ttacatgata cgcgtaaaaa acagctacca
     6181 gatcgataaa gtttttgctg cgatcatttg tgtcattcta ttgagtttat tgctgaatgg
     6241 cgcttgttca ttattgaaaa aagggtatta tcgtatgctt tatatttttt aagagaaggg
     6301 gatcaaatga aaaaattatg gcttttatta ccgctactgt tgcttttatc cgcttgtggc
     6361 actgcaaaag aaacaagtag caaacaagaa ataaaggatt tgaaaaaagt gacacttgtt
     6421 ttggattatg taccgaatac taatcatact ggcatctacc tagcaaaaga aaaaggctat
     6481 tataaagagg ctggtttaaa tgtccaaatc atcgaaccag gagataattc gacaagtatt
     6541 ggtctagtag gtgcggacaa agcacaattt ggtgtaagtt atcaagaaga tgtcacttat
     6601 gctcacgcag atggacagaa tataccagtc aaagcaattg ctacagtgat caaacataat
     6661 acttctggat tcgccaccct atcagacagt aacatccatt caccaaaaga ttttgaagga
     6721 aaaacatatg caggttggca aagtccaagt gaagaagctg tcttaaaagc tgtgatggaa
     6781 aaagacggcg gggacttttc aaaactgaca atggtaggaa gcaatggaga agggccagaa
     6841 agcttaggaa aatcctcaga tatccaatgg tactttgaag gttgggatat gatcaaagca
     6901 aaagaagcgg gtattgaact aaactatatc ccattgaaag aattagatga acggttagat
     6961 tactatactc cggtaattat tacgaatgat caactgatca aaagtgatcc agaattggta
     7021 cagtctttta tggatgccac gaaaaaaggc tatcaggaag ctattaagga tccaaatgac
     7081 agtgctaaac tgttgcaaaa atatgcaaaa gaaaatgatc ggacgttttt agaagaatca
     7141 caagcattct tgtcaaaaaa ttatacggat gatcctgaga attggggatt gatggaagaa
     7201 aaagtttggc gcaattatac agcttttatg caggaaaacg gattgatcaa gcaagatgta
     7261 ccttcgcaag aactattcac taatcagttt ataaaatagg agtgatagat atgtcaatcc
     7321 aaatcagcca cttgaatgta gtcattgaaa acctgaaaat attagaagac atccatttca
     7381 ctatccctaa agataccttt acatcgattg tcgcgccaag cggagccggt aagtcaacat
     7441 tgctgaaaac tttaacagga gtccagccta tgacttctgg aacgatcaaa gtagacgatc
     7501 aaaaagtaac tggtctccat acagcattta gttatatgcc gcaagaagat atgttacttc
     7561 cttggctgag cgtctatcaa aacgttacgt tatatcaaaa aatcaatcat ctaacaatca
     7621 atgaagaaca agtaagggat tatctggata tctttgggtt aaacggctat gaacattttt
     7681 tgccggaaca attatctggc gggatgaagc agcgaacggc cttgttacga acaatcatga
     7741 atccttcttc ttacttgctt cttgatgaac catttggagc gttagatgct atgaccagag
     7801 ggcagatgca agactggatg ttgaagctgc ctaaaaaagc aaagcggact actttacttg
     7861 tgacgcatga tatcgaagaa gcaatttatt tatcagacag gattcttgtg ctttccgccc
     7921 gtccggcaca tgtgattgct gagattcaag tgcctgaaaa acgacggtca cgtgaatggc
     7981 tactgcagca atcagagttg aagcaaacga tttatcagtt attggcaggt gaattagatg
     8041 ttaagtgatg ctcattgtca tttagatggg agtcagagtt tagcactcct tcagcaggaa
     8101 cacacgattt tgacgatcat caattgtgat tcgccagaag aatggaagga aaacagacag
     8161 cttgcagcaa gtaagactca agccttaagt tacggtatcc atccttggaa agcagatagc
     8221 tatacgtttg aacaagttga gccttttttg aaaaaagcaa ggatcatcgg tgaaataggg
     8281 ttagataata tttggacgaa tgtaccgatg actacacaaa aaaaagtatt tgagcgacag
     8341 cttgcttttg cagccataaa tgagaaacct gttgtactgc acacaaaagg atgcgaaaaa
     8401 gagatcttaa attatatcca agcttaccct aatcactatt tgatccattg gtattcttct
     8461 ttggactatc aacaagacta tattgattta ggctgttatt tcagcattgg tcttgatttg
     8521 aaaaagaatc cagcagtctg gcaactagct aaagcagtcc caatcgacca cttacttatt
     8581 gaaacggatg gactacatgc agcagagtgg gctttagggg ggattttttc agaaaaagat
     8641 tatgtctcac tgcttgaaga acagttaaag acaatcgctg agatcaaaga gctatcagtt
     8701 gaagaagtta aaaaaaatac ttatcaaaac ttggaacgtt tcattagatt aggtaaataa
     8761 aaaagagatg tgacacaact agtgtcgcac ctctttttgg ctttctttgt ataaaagtaa
     8821 actgtttgat taataaatga atcttagtac caaccgtttg cttcccagaa tgctttcgcc
     8881 gcttcccaag aaccataacg tgaagtaaca tattgttctg ctactctttc ttggtttgca
     8941 gcagagtagt caccatttaa gtaagaagaa tctaattggt aacgtccgat gtaacgtccg
     9001 ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga tccattcttt tgcagagctt
     9061 gtgcttgctg gagtagcagc agtttctgtt acagcaggag caggttgtgt ttcagcaaca
     9121 ggcgctgctt gtttttccgt ttgaaccgtt tcttgtacaa caggttgttc agcttggact
     9181 ggtgcttctt gaacggctgc ttgaggttca gcagctgctt gtgcagagcc ttctgttggg
     9241 attgttaatt gttgtcctga gta
//
LOCUS       DAYYME010000107         9224 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000107 DAYYME010000000
VERSION     DAYYME010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.108"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(104..2197)
                     /locus_tag="ACHBFW_002195"
     CDS             complement(104..2197)
                     /locus_tag="ACHBFW_002195"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM9318470.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(2946..3569)
                     /locus_tag="ACHBFW_002196"
     CDS             complement(2946..3569)
                     /locus_tag="ACHBFW_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318471.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(3728..5125)
                     /locus_tag="ACHBFW_002197"
     CDS             complement(3728..5125)
                     /locus_tag="ACHBFW_002197"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9318472.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(5157..6128)
                     /locus_tag="ACHBFW_002198"
     CDS             complement(5157..6128)
                     /locus_tag="ACHBFW_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9318473.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            6502..6927
                     /locus_tag="ACHBFW_002199"
     CDS             6502..6927
                     /locus_tag="ACHBFW_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318474.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(7415..8116)
                     /locus_tag="ACHBFW_002200"
     CDS             complement(7415..8116)
                     /locus_tag="ACHBFW_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM9318475.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(8132..8572)
                     /locus_tag="ACHBFW_002201"
     CDS             complement(8132..8572)
                     /locus_tag="ACHBFW_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM9318476.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(8572..9147)
                     /locus_tag="ACHBFW_002202"
     CDS             complement(8572..9147)
                     /locus_tag="ACHBFW_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFM9318477.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         2670 a         1893 c         1517 g         3144 t
ORIGIN      
        1 aaggaaaaat acacgaaaaa aataaaaaaa tgtttttttc ataaaaaata ccaaccttca
       61 agcattagaa tgttgtctaa ctactgaagg ttggtttgat tgtttattga ttattttttt
      121 gctgttcaac gtctgttctt gttactggat ataagttggt tgttgtttta ttaccatttt
      181 ttgaaaagac acttgtcgaa tgttcgcctt tttgcttttc gattgcttca ttacgttccg
      241 cttcattttt atagtcatat tttttcggat caatcgtctc aaatccgttt ggcacataaa
      301 accgcaagag attttcctga ttcagcttat ctgaaagact taacgcagtc ttgacttttt
      361 cctgatcttt ggagatttcc tcatcgattc ctgctgcttt cgtatcgatg atttctcctg
      421 tctgattatt gtaagctttt cctcctagga cggtgtattt gggtgtcaca aagttcccat
      481 ttcgaaaagc aactacttga tcatgctgag aagataataa gtctgtacca aaatgaagat
      541 aatctttatc atcgattccc aatagatgta gtaatgtcgg caaaacatcg atttctccac
      601 cgtattcatg attgactgtc cctttagtat aaccaggcat atggatcatc aatggcactc
      661 gttgcatctg tgcgttgtca aattcatccc atgtatctgg atctttacca agagcgcttg
      721 ctaaatcttt attatctgta ttcgagatcc caaaatgatc cccgtaaatc acaaagattg
      781 aattttggta aataccagat tttttcaaat acgtaaaaaa ttgttccaat gattgatcca
      841 gataatgtgc cgttcgaaca taattatcta ctgtgctatt gccagtattc aaagatggaa
      901 agtcgaaatt cttgtcatcc gtataataag gcgtgtggtt ggttacggaa agaaatttcg
      961 tgtaaaacgg ctgttgtaga tgctcaagat attgggcaga ttctctgaat aaatctttat
     1021 ctaatatccc ataacctaac gtttcgtcgg attcatcaaa ataagatcga tcaaagaaat
     1081 tgtcataacc taagttttta tatacatgat ctctattcca aaaactagct acattcccat
     1141 ggaaaacagc gcttgtatag ccttgctttt gtccaaggat cgctggtgcg gcttgaaaga
     1201 cattgtctga cccaagttct gtgaataatg agccttgagg taaaccaaat gtgccagttt
     1261 ccaacatatt ttcagcatca cttgtctttc cctgtcctac ttcatgaaag aaattgtcaa
     1321 agctgatcgt tgcttgattc tggaaaatac tatttaaaaa cggtgtgact tcttgtccgt
     1381 ctaccttcat attaatcaaa aactgctgga aactttctaa atggagaacg atcacatttt
     1441 tccctttagc gatcccaaat gtttcgggat ttggctctgc ataatgtttc tttgtataat
     1501 ctaaaacttc atcaatccca ttactgcttg catgcgcgcg aacactgcta gtcattcctg
     1561 ttttgattcc gtcataaata gtatacgcat cgattcctag atatttgaca atatatgaac
     1621 ggtcaaatgt tcgctgcagc aattgtggac ggttggcttc gctcagcgct agatttcctg
     1681 aaaaaataag ccctgaaaga caggtaacag cgatagccat cggtttgccg acagggttgc
     1741 ttttgatagg tattttcttg accttcaacc aaaccagtag cccaatgaac acagcgatat
     1801 ccagccagta gatgacatca tggggcttca tcaaagaaaa ggagcttcct gacaatcctt
     1861 gcgacacttt cgaaaagcct aatacagatt tgactgtaat gaaatctgtg aattcccgat
     1921 aaaaaataat attcaagtac aacaaaagcg tatttaaaat atcaatgatc aaaagaatag
     1981 ctaacgctgg ctttggcttt ttaatataca aagaaaggcc aaaaaacaat aaggtagtcg
     2041 caattgggtt gatccacaag ataaagtact ggatcgtacc ttccacacct aatgaaaaat
     2101 ctgcatagta ggcaatgatc gtcttcagcc agaaaaagaa aacagctgtc aaaacgattg
     2161 ctaaacgatt ttggcacaat tttttaagtt gtgtcacgta tatcacttct ctctattcac
     2221 attttttctt attttgctcc tgaaaaatga aaatttccta aaaaagttgt tatcttaaga
     2281 tttttttatt ctttctaaag attattccgt ccacttttca cagcataaaa ccgctgcgat
     2341 gtaagcgatt aaactaatga agagataaaa gtcttgtggt gaccgcttca ctccacggta
     2401 ttcaaaagcc agtttctcgg tattcgtaca aaaaaagagg ctgtgacaaa attcttgtcg
     2461 cagctcctta tttcttgatg caggacagct ttttcacaac ttcttcctta aacggtgttc
     2521 tcctgccaac tcctcggcca caagtcacaa tatttataaa acatgagaag ctgttttttg
     2581 aaattgttcc ttgtggctca gagttaaacg gcaggctcaa gacctcttga tacacggtgt
     2641 tcaatcagct gaccctcggt attagattat gtcaggacgc taattttaca acctcttggt
     2701 aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa aaatataagg
     2761 agctattttt tgaaattgct ccttgtggct cagagttaaa cggcaggctc aagacctctt
     2821 gatacacggt gttcaatcag ctgaccttcg gtattagctc aaaaaacggc aaaatatgag
     2881 gaactatttt gccgtttgtt tctctaatac tcaggtcaaa acgctgattt cacaacctct
     2941 gtttttcatt gtccatagga ttgatattgt ccttgtgatg actcattcgt aataattgag
     3001 gcttgatttt cttcacttcc cgttatcgtt tgtccactgc cagaatctga aggacttgtt
     3061 cctgtcgact cgtttttttg agaagagaca gttgttttct tgacttgttc atttgtcttt
     3121 ttccattccg ctaaacgatc ggcgaccact tttttgtcta ctttttcaga ataagcggtg
     3181 atcacttttc catttttcaa acgggctact ttgtcgccat catgagtcaa aagcaaaacg
     3241 gtcaaatgct tgtctttaaa ttgatattgt ctgagagaaa tcgcactaag tacgtcatcg
     3301 atattaataa attgattttc ttcttttcct tttggcaaat aaatgtccaa ttcgcctttt
     3361 tcgtaattgg cagttatttt atactccgcc caccaataag ttgagaattc ttttttgaaa
     3421 ttatctactg attctttgct tgctacgatt ttttctgttt ctttctgagc aagatctgct
     3481 gtcatttttc ctgaaaatgc tgcggataaa ataagcagta agaaaaaacc gaataaccaa
     3541 aagagaagaa acggtctttt tttcctcatg ctttcctcca ctcctattta attttgacta
     3601 ttctattata tacaaaaaat gaaatagaaa gacaaaaaag ttgaaaaaga tcgtaaataa
     3661 ttgaacttct ttttcacaaa atggtaagaa tggagaattt cacgcatttg ctctcaacta
     3721 ttctttctta agcctcaaat ccgtttttat ctgatacgtc tttgaaccaa tgacccgatt
     3781 ttttgattgt tctttttcca tcttgtgcta agtctactga aataaagcca tatcgattct
     3841 tatatgcatt ggtccacgac cagttatcca tacatgtcca catatgatag cctaaacaat
     3901 tggcaccttc ttgaatggct tgatggacat atttcagatg agattggaca aattcgatcc
     3961 gataatcatc gtggatcatg ccatcttctt tgataaatcg tgcttcgttt tctacaccca
     4021 taccgttttc agaaataaag cagtcgatat ttccataatg atctcgtaca ttgatcaaaa
     4081 tatcatagat tccttgttca tagatctccc atccgcgata aggattcatt ttacgaccag
     4141 gcatttcata attgtcgaaa taatcctcag gcattggacc gtttttagta tcgattggtg
     4201 tttcttttgc ttttacccgg cgtggctggt aataattgat acctaaaaga tctactgtat
     4261 tttctttgat aaccgtcaaa tcctctgcat ggatcacagg catgagatca agttctttta
     4321 ctatctcaat caattcactt ggaaactcgc cttttacagc tggatcaagg aatgaacgat
     4381 tgaagaacgc atctgctatt tgcgctgctt taacatcttc agggtttgat tcgtctcttg
     4441 gatagcttgg tgtcagattc aagatgatac caataacacc accttgctcc atttcatggt
     4501 aagatttgat ggctaacgct gaagccaacg cttcatgata gccaacttgg atcgcttttt
     4561 tcatatctga ctcttctgga taatgccatc gatataaata acctgcttcg actggaacga
     4621 ttggttcatt atgagtaaac cattttttta ctttcgaacc aaacaattca aaacaacttt
     4681 tggcatattt ttcataggct tcaaccgttt ctcggttcaa ccatccgcct ttttcttgca
     4741 gatacatcgg catatcgaaa tgatacagat tcacaaaggg ttcgataccg ttttcaatca
     4801 actcatcaaa ataacgatga tagaaacgaa cggcttcttc gttgattgtt ccagattcat
     4861 caggtatcaa acggctccat tggatagatg ttcgaaatga attatgccca gtctgtttca
     4921 tcaactgtac atcctcttga tacttcgtat aaacttgcga ggttttttca ggtcctactt
     4981 gatcaaagaa cttttgcggt tcttttttgt accaataatc aaaaatgttc tctccttttc
     5041 catcgcctga aatccttcct tctgtttgtg ggccacttgc tgcagaaccc caccaaaaat
     5101 ttgctggaaa ttgatatttt ttcatcaaag cacctctttt tcttttttag ttcgttttag
     5161 ttgattccat gtatgatatt ttccatcaca atcacttttt cattttcttc ctcttctttt
     5221 ttgattcgtt ttaacagctg ttctgctgct cgttccccga ttttatctgt tggtggcgtg
     5281 atcgtgattt tcggaaggat actcagatcg tttttatcta aataatcata gtgactgaac
     5341 gcaatatcct tcccgattct gacaccttct tgattgaaac attcgatggc accaatcgac
     5401 atattgtaat tgctgacaaa aatagcagtt gccccttttt ctaataatgc ttttgccccg
     5461 taatagccgc tttttctaga atagtctcct gtaaagacct ctaattggat cgatggatca
     5521 ttttggaccg cttctttggc tcctgctaaa cgttctcttg ctacgtagtc tgtttgagtg
     5581 gcagcaataa atccgatttt tttatgtcct tgttcaccat gatgagcgat cacttttgct
     5641 acactttctc gatcattcac taatattgta tcaatacctg ctggtccttc tggacaattc
     5701 aatgcgataa ctggaatact catttttgtg agcatttcaa atccagacca ctcttcttcg
     5761 gataagaaga caatcagccc atcgatcaca tgcttcatca ggtattcaat tttttgttca
     5821 attttttctg gctgatcggc aaacccgctt aataataagc tatacccatt ttcttcagct
     5881 actctttcga tccctgagac aacttccgac ccaaaacggc tggaaatccc gtttatcaaa
     5941 agtccaatag agaagcttcg attattcttc aaccctttag caatgatgtt ttctttatag
     6001 tccaacgctg aaatcgcttc tgctattttt ttcatgttct tttccttcag ttgctgacca
     6061 ttcaagtaac gggaaactgt tcccactgaa acaccagcca tcttagccac atcacggatc
     6121 gatgccatac attctcactc ctgccagtta catttgattt ctctttcttg ttgttcgatt
     6181 atgatgaccg cattctttgc atagtgttgc ttgtcatcaa tatccatata actaaagtct
     6241 tcagtagagc aacttcctct cactattaat tatacttaaa caagtatttg aaccggttca
     6301 tttttaatat agcatttttt taaaagattg caacactttt ttaaaacgct ttctgaaagt
     6361 ggcggatttt ttgccttttt aaaattttat tctaaaattt actctatttg tgggtgtttt
     6421 gtgcgttttt ctcttccatc cgtttgaagt tttacattcc aatggtataa tttctttatc
     6481 ctatcaaatg ggggaagtat tttgaagaaa ttaatcgtac tatttatttt gtctgcggga
     6541 cttttgacat tgactggctg tgatttgttt ggcggctctg accaatcctc ggacgaaaca
     6601 accgattctt ctgtcttaaa agcggaaaaa gcagatacca ccaacagtgt atatacgcta
     6661 aaagtacgta aagatatgga tttatccgcg ggacagttga catttacaga taaaaaaatg
     6721 gaatggacac gcacgtatca gacacaagga actgattctt ctgaatcatc tgaagaaaaa
     6781 acagtgctga cagatatcaa gatcactacc aaagatgacg aatatattat ttcaggaaaa
     6841 gaaaataaaa aagaaacaac agtcatattt acaaaaatcg gtaattaccg tttgaaggat
     6901 gaggatggca acatctacag tctatagtgc cgtctgttgg atcttctttt gtttctcccc
     6961 ttcttttctg ctatagtaaa gaagcaagat gattgtaaag gagacgaaat ggatgatcga
     7021 tagtcctgat tattgtacat gtaataacaa caaaaatatc catacagaat ttaatgacca
     7081 aggaagcttg agcatatgcg acaactgtca caaactagtc aaagattcat ttaaaacatt
     7141 tgaatcttat cctgttcaaa attatgtaaa aagtagaaga agataaacaa atccaagcaa
     7201 aactcactat ggctgatttt tatgaacttc aaactcagct tcttcctttg atcaagcttc
     7261 atcagtaaaa caaacagtgg atcatttcag tttacaaatc aaagagtaaa gatttattta
     7321 tttcaaatat ttgaatataa aataaccaaa cgatagaaag gcttattcta catcacgtag
     7381 taatttttct ttcccgtcgt ttggtttttt atttttagcc taattcttga ccttttgcat
     7441 gctggttatc cgtaaaatat tttccagcag gctttccttc caattcagct aatttttctt
     7501 ggatctgacg gatcaccttt tttgcatctt ctggattttc tacaaaatcg aatttatccc
     7561 catccacttg tattttagga catacatcaa aatcttcata ccattgatta tagcgggtgt
     7621 tcaacgtttc ataataggta tataacgtct catcgctttc tagctgttca tagtcacgcc
     7681 cacgtttttt gatacgttct agcatcgtgt ccaaagaaac tttgatatgg accatcaaat
     7741 caggccgttt ttttggagca acatcatcta actcgtttag catcgtatct aataagctat
     7801 cgtactgctg tacttcgata tccgttaccc gacctaaatc agcgtttaga tggaacaaca
     7861 ggctatcttc atagatggat cggtctagca cattgtcatc gttcactaaa gcatttttca
     7921 ttgctaaaaa tcgcttgttc aaaaagaaga tttgcaataa aaaagcgtac tgctcaggat
     7981 ttgaataaaa gagcggcaat acttcgttat cttcgacatt ttcataaaat ggtttagaat
     8041 caatctcttt tgcaagcata tccgtcaaac tgctcttacc tgcaccaatc gttcctgaaa
     8101 tcaaaatcac tgacatacaa cccttcctct tctataagta ctcgcctacg taagtttcaa
     8161 caggcaatgt ttcaaaatca tattcacgta cagcttggtc tgattttaaa tacgtatgga
     8221 gactttgtgt aatcatcaag ttcgtaggta ccgtctcttc ttgtacaatg atcatcggtt
     8281 ttttcaacat agttgccaca cctaattcgt aagccgttcc tggatcgatt gtttgatgct
     8341 caaagtcaag gatagcaaca acaaattctg catttgtcag ttcttccata tctttttcgt
     8401 atacttcctg cgcccatcca gcgctgaata gttcataatt gctttcttgg tgatgacgtg
     8461 gagagtagaa acttgtgact gttttgtttt cctccaacgc tttttcgata cgacttactc
     8521 gatcgatttg ctcctctgaa aaaaatggtc ctgctaaata tatttgactc attattttgc
     8581 ctccttcaat aataactgat ttttaataga tactgctcgt aattcattga ccagttccaa
     8641 tggattgttg gaaaagatta cttgatccaa ccatttttta tccataaaat cctcttttgc
     8701 aaaagtttct aataattgtt gcaagccgct gtaataaccg ccaatattca atattccgat
     8761 tggcttgtcg ggcgtcagac cgattgctat ccaagaaaga agttgtgaga attcctccaa
     8821 tgtacctaga cctccaggga aaacgaggac agcatctccc ttttcaaata ataatacctt
     8881 tctttcatcg atcgtttctg taggaataaa tgtagtaaca tcttttccag gcaattcagt
     8941 ttcaaaaagc ccattcggat agatacctgt gacttttccg ttattttcca gtactgtttt
     9001 actaatcact cccataatcc cggagtatga accaccgtaa accagttcga tttcttcttg
     9061 tgccatgtat tttcctaacg tttgcgcaat aaatttgtaa ctttctttat taccaaaacg
     9121 ggaaccgcaa aaaacagtaa gtttcataaa aaaacactcc ctaactcaaa agtctatctt
     9181 cattctacac gtgttttttt aaaataaaag actagatttt gtag
//
LOCUS       DAYYME010000108         9130 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000108 DAYYME010000000
VERSION     DAYYME010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.109"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..583
                     /locus_tag="ACHBFW_002203"
     CDS             <1..583
                     /locus_tag="ACHBFW_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM9318478.1"
                     /translation="YKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTN
                     IVSPAVRGVFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRV
                     ADQSPITINLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLD
                     VYENAELKIEFSKQNHYFFVEMYLASRKSKSAY"
     gene            905..1132
                     /locus_tag="ACHBFW_002204"
     CDS             905..1132
                     /locus_tag="ACHBFW_002204"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM9318479.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            1255..1581
                     /locus_tag="ACHBFW_002205"
     CDS             1255..1581
                     /locus_tag="ACHBFW_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM9318480.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            1649..2362
                     /locus_tag="ACHBFW_002206"
     CDS             1649..2362
                     /locus_tag="ACHBFW_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM9318481.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            2672..3522
                     /gene="licT"
                     /locus_tag="ACHBFW_002207"
                     /pseudo
     CDS             2672..3522
                     /gene="licT"
                     /locus_tag="ACHBFW_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            3643..5508
                     /locus_tag="ACHBFW_002208"
     CDS             3643..5508
                     /locus_tag="ACHBFW_002208"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM9318482.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAVCISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            5522..6965
                     /locus_tag="ACHBFW_002209"
                     /pseudo
     CDS             5522..6965
                     /locus_tag="ACHBFW_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            7073..7774
                     /locus_tag="ACHBFW_002210"
     CDS             7073..7774
                     /locus_tag="ACHBFW_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9318483.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            7900..8412
                     /locus_tag="ACHBFW_002211"
                     /pseudo
     CDS             7900..8412
                     /locus_tag="ACHBFW_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(8810..9019)
                     /locus_tag="ACHBFW_002212"
     CDS             complement(8810..9019)
                     /locus_tag="ACHBFW_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFM9318484.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
BASE COUNT         3086 a         1496 c         1994 g         2554 t
ORIGIN      
        1 ttataaaagt ctactgtttt cattaaaaat tactcttcag gaaaataaaa aagaaacgtt
       61 ggaattttta gaaatattag aagatcattc tgcagctatt tttgaaaaac cgcaaataca
      121 acagctaaca aatattgtat ccccagctgt caggggtgtc tttttaaaat ttctagaaaa
      181 agcagaatcg gagaaaatcc cgatccaagt agttattcct gtcgaagtga aaacaatccc
      241 gattgatctg gttgttttta ttcgctgttt atctattttt atttctaacg cattcgatca
      301 tcgtgtagcc gatcagagtc cgattacgat caatctgctg gaagaaaaag agggatcacg
      361 ttttagtata gcgaacaagg caaatccaac gaatttgacg ctatcggaga tggtcaaaag
      421 aggaaaaacc tctaaaaaaa atggcgggtt aggtctatac agtgcgaaaa aaatgttgga
      481 tgtttacgaa aatgcagaac taaaaatcga attttcaaaa cagaatcact atttttttgt
      541 agaaatgtat ctggcatcta gaaagagcaa atcagcctac tgacttactg tcaaaggata
      601 agtttgagga aagtctacac ggagatagag acaacaacac agcatattca aaagaaacag
      661 aagcgtgatg accgttgtca cccttctgtt tcttttgcta tgtagttaaa caaagtagtt
      721 gacacttccg ctacttagta ttagtacgaa agtatacttc atgatactaa gtagataaga
      781 agtgaaaagc ggaacccacg atcgtcaaaa tgctaactgc tttgcttaca ccagataaag
      841 ggaagagcaa tatcgcttat tttaggcgtg gagaagcaag caaatcccta cgatcacaaa
      901 aaaaatgctg acccagcagt taagagagtt agaacaagat gaaagtatcc atcgaaaagc
      961 ctataaccaa gtcccgccta aagtagagta ctcgctcacc gaagaaggaa aatcattaag
     1021 agagattcta gtagcgttgt ctgtttggga agaagaacgt atcgaaaaac aaaaagcctc
     1081 aagggaagaa gacacgctac tgcatacaca tggtggctac ctaaattatt gattttctat
     1141 gacatttatt ttaaataaaa gaaaacagga gtttttttaa aagcatcatc tattttttat
     1201 gtaaaaaaat ggtatgatta atcaagtaag aagtgattgg aagaaggcaa gcaaatgaat
     1261 agtatggaaa aagtcaacca gtttagagat gaacgtaatt ggcgacaatt tcataacgaa
     1321 aaagatttgg ctatctctat ttctttggaa gcatctgaat tgttagaact ttttcaatgg
     1381 aaaacgccgg aagaagtaaa agaaacacaa ttagaacgaa tcaaagaaga actggcagat
     1441 gtattgatct attcatacat gatggcagac aaccttcacc tggatctaga tgagatcatt
     1501 gaagaaaaat tgatcaaaaa taatttgaaa tatcctgtag aaaaaagcgc aggtgtacga
     1561 aaaaaataca cagagttgta gaaatgtgtc attcggctgt tggaaagagc aaaagaaaaa
     1621 cagtaaaatg agtctggagg gagagaaaat gaaaaaaaaa ggaaaagcaa aaagatggct
     1681 agtcaatatc ctcttgttcc tcttgttatt agtaggacta gctttgattt tcaatgaaca
     1741 gatcaaggat tattttgtca gagagacagg agataaatat gctatagcga atgtaacaaa
     1801 agaagatctg aaaaagaata acgataaaga tgtcagtttt gactttgatg cggtagaacc
     1861 aatgacgaca gaaggggtca tgaggtcgca aatgagagga acagatctac cagtgattgc
     1921 tagtatcgca gtcccttcag tttcaatcaa tttaccggtt ttcaaaggct tggataatac
     1981 gtcactactt tacggagcag gaacattatc tccagatcaa gaaatgggaa aaggaaatta
     2041 tgccttagca agccatcgtg caacgaaccc tgaattgctg tttacaccgc tagaaaattt
     2101 agagatgggt gccaagatct atttgacaga tttagaaaat gtgtatacat acaaaacatt
     2161 ttttaaagaa aaagttgctc ctacagatac ccaattactg aatgaagtcg agggaaaaga
     2221 aattgtcaca ctaattactt gtggcgacat ggacgccgtc acgcgactag ttgtacaagg
     2281 agaactggaa agtgttacat cgataaaaga tgcgacagat gatatgagat ctgctttcaa
     2341 tctagagaca aaaacatttt aggatactgg gacataagtc gttacagatt aaaaacagcc
     2401 aaaggatatg aatcgataag gaaaaaccaa tttgctgaaa aaaagagaaa cgcaaagagg
     2461 tgcatctctg atttggttgt tttctagata gacttgtgtt ccagtttttt tattttttat
     2521 atgaaaaggc ttgcataaat aaaaattcga tggtatgatg attttgaata agaaatggat
     2581 tgttactgta cggcaggcaa aaccagaatt cagctaagaa acagtaggcg aattctaggt
     2641 ttttttattt ttcttgagag ggcgaagcaa aatgaaaatt gataaaattt tgaataacaa
     2701 tgtcgtcatt tcgaaaaatg gattcgggga agaagtcgtt tgtatgggaa aaggactagc
     2761 ttttcaaaaa aagattgggg acgagatttc gccagaagcg gtccaaaaag aatttgttta
     2821 agagattctt ttgctaaaaa ccaattccag cagctgatgg ctgatattcc ggcagaggag
     2881 atcgaatggg tcaaacaagt agtcgaactg gctgaagata aactaaaagt tgaattttcg
     2941 cctaatattt atctgacatt gaccgatcat cttcattatg cgatcacacg agcaaaagaa
     3001 aatatccaac tgcctaatcc attattattt gaaacaaaaa aattttatcc gaaagaatat
     3061 caagcagcga aagaggctct tttgttcgtg aaacaaaaga cgggtgtgga actttctgag
     3121 gatgaagccg gttttattgc ctttcatttt gtgaatagcc aacaaggaaa cgaagacatg
     3181 caagtgacga tgaccgctac cacaatggta agagatatac tgagtatcat cagtaaattc
     3241 ttcggcatcg tgttcgatga agaatctttg aattatcagc gtatcattac tcatctgcag
     3301 ttttttgccc agcgttatct caaaggggaa ggatctgacg agcaagatga atttctctac
     3361 gcattagttc aaggaaagta tccaaaagcg tttcattgtg tcgaacggat caatgagtat
     3421 cttcgacaaa cgcaaaaaca ggaaatgggg atcgcagagc agatttattt aacgattcat
     3481 attcaaagag tagtaagtga gaaaaaaagc atccaatcat aaggattgtt actgtatggc
     3541 aggcaaaacc tggattgaaa aaataggttc acgaatgttt gtgactgttt ttttcaatct
     3601 gggtttttat tttttaaata aaaaagaaag aaggaaaaaa caatggataa tcaagcagtt
     3661 ggtcgtcgtg tatgggaagc ggtaggagga caaaagaatg tcaaaagttt agtacattgt
     3721 gctacgcggt tgcgattcag attaaaagat gaatctttag cagatacgca aaagttaaaa
     3781 gaagacccgg acgtgatcca agtcgtacaa agtggcggtc agtatcaagt agtgattggc
     3841 agtaatgtag cagatgttta tcaatcgatc gtggatgagg aaggtcttgc agtttcggag
     3901 gatggaaaag aagagtccaa aaatattttg aatcgtctga tcgatattat ctctagtata
     3961 tttacacctt ttctaggggc gatggcagcc gccggggtat tgaaaggatt cttatcattg
     4021 gcaacggtga tgggctggct gacagctgat agcggtactt atcaaatatt gtttgctgca
     4081 gcagatggtg tctttacctt cttacctgtc atgcttgctt ttacagcagc taaaaagttc
     4141 aaagccaacc agtttttagc agtagcaatt gctatggcac ttgtgtatcc agctatcaca
     4201 gcagttgctg gtgcgggaga agctatcagt ttctttggga tcccagtgat tctttcacca
     4261 tctggttata cttcttctgt cataccaatc attctagccg tatgggttca aagtaaatta
     4321 gagccattcg taaaaaaagt gattccgcaa tttctgcaaa tgattttagt tccgcttgtc
     4381 gttttagtag tgatggttcc attaacattt cttgctttag gaccaatcgg gacggttgcc
     4441 ggtaatgctt taggcggtct cttcaactcg atttatggat ttagtccaat cgtagcaggt
     4501 ctaatcatgg gaagtttatg gcaagtattc gtgatgttcg gcatgcattg gggattcgta
     4561 ccgatcatgt ttttgaatat cgaacaatat gggtttgatg tattgatgcc aatgcttctt
     4621 ccagccatct tggcacaagg tggggctgct ttagctgtag cattgcgaac aaaagatacg
     4681 aaacttcgag cattagggat ttcttctaca gtgacttcgt tatttggaat cactgaacca
     4741 actgtgtatg gtgtgacatt acctttgaag aaaccattca ttgccgtttg tatttctgga
     4801 ggtattggtg gagctattat cggattttcc ggagtaaaag ctttctccag cagtttggtc
     4861 agcttattga cgattccgac tttcattaat acggtggatg gtgtagagtc aaatgtgaca
     4921 gtagcagtaa tagctacagg aattgctttt gttcttgcat tcgtaggtac attgatttta
     4981 ggatttgacg aacaaacaca agacaatcaa ttagaaaaca aacatgcgaa tgcaggagaa
     5041 ccgattactt cagcacggca taccttaaaa agtcctttaa cgggtaaagt actgccattg
     5101 agtgaagtac cagaccaagt tttttcttct ggcgtgatgg gaaaaggtat cgctattgat
     5161 ccagaagtag gagaactagt tgcacctgca gacggagaga tcacgactat ttttccaact
     5221 ggacatgctg tagggatcac aacgactgac ggagcggaga ttttgattca tatcgggatg
     5281 gatacagtag aattaaatgg gaatggcttt gagatcttag tcaaacaagg ggatctcgtc
     5341 aaagcaggag atttattgat tcgttttgac atcgaagcaa ttagagcagc tggctatagc
     5401 gtaatcactc cagttgtcat tacgaatact gatgcatttg ccgatatact tgaattagac
     5461 caaaaagaga ttattgcaaa cgaagatgtt ctggcaattg taaaataagg aggaaatcag
     5521 gatgcaatca cgtttttcag aaaaattttt atggggtgga gcaactgctg ccaaatcaat
     5581 tagaaggggc ttatgatcga gacggaaaag gactatccgt tgcagatgct atgcctggag
     5641 gtaagcagcg ctttgcgatt atcggcagtg aagaatttga ctggacgatt gatcaagaaa
     5701 aatatattta cccgaatcat cgaggaatcg atcattacga tcgtttcaaa gaagatcttg
     5761 ccttgtttgc ggaaatggga ttcaaatgtt atcgtttttc gattgcctgg acacgaattt
     5821 ttccaaatgg agacgaaggt actcctaacg aagcgggatt agaattttat gatcagctga
     5881 ttgatgagtg cttaaaatat gatattgaac ctgtgatcac gatttctcat tacgaaatgc
     5941 ctcttcattt ggcaaaagaa tacggtggct ggaaaaatcg aaaactgatc gacttctatg
     6001 aacgatttgc tcagacagta ttagagagat acagcagtaa agtcaaatat tggatgactt
     6061 tcaatgaaat caattcggcc tttcactttc cagctttaag tcaaggatta gtaaaaagca
     6121 atggagcagg agagtaccaa aatattttcc aagcatggca taatcaattc gttgcaagta
     6181 gcaaagccgt aaaaatcggc cacgaattgc gttcggatat ccaaatcggc tgcatgatta
     6241 tctatgcaac aacttacagc atcgatgcca atccagtcaa tcaggcagca acaatgatcc
     6301 aaaaccaaga attcaatttt ttctgcacgg atgtccaagt acgtggagaa taccctgcat
     6361 acacagcgcg tacccataaa aaatatggag tcgatccgga aaaattagag caaacggaaa
     6421 aagacttcca gttgctaaaa gaatatccag tggactacat cggatttagt tattatatgt
     6481 ctacggcaat caatgagact gatccacaag cagcaacttc agaagggaac ttgctgggag
     6541 gagtaaaaaa tccgtttctt gaggcaagtg aatggggctg gcagatcgat ccagaagggc
     6601 tgagaatcgc attgaatgaa ttatacaatc gttaccagaa accattattc attgttgaaa
     6661 acggtcttgg agccattgat catatggaag aggatggcac gattattgat gattatcgga
     6721 tcgattattt acgtcgtcat atcgaagcga tggctaatgc agtagcagac ggtgtagatt
     6781 tgatgggcta tacgccttgg ggatgtatcg atcttgtcag tgcatcaaca ggagaaatga
     6841 gtaaacgtta cggctttatc tatgtggatc tagacgatga aggaaatggg acactgaatc
     6901 gatcaaagaa aaaatctttt gattggtata aagaagtaat tgcctcaaat ggcgtaaact
     6961 tgtaattaaa gcagctgaat gaaaaagaac acacttctca catgtgattt gtgttctttt
     7021 tttatttttt atgtgctatt cttttagtaa tagtaaagca aagggtgacg ttatgaaaag
     7081 gacacgtgta ctatacttgg aagtcgcaga taaaattaaa gaagatattt tctcaggaaa
     7141 atatccagtc ggttcgatgt tgccgacaga aagtgaatta gaagaactgt ttcaagtcag
     7201 caaaatcact gtccgaaagg caatcgaact gctggcatca gatgaatatg tcgcaaagaa
     7261 aagtggtcgt gggacgacgg ttctaagcaa tcgtccttac aatcgattat ccaaagcggc
     7321 aagctttacg caaatcttgg agaactcccg atatgaagtc cggaaagaaa cgctggattt
     7381 gcaattggtc catataaaga aggaagatcc tttgttttcc tttttcggtg agcaagctgt
     7441 ttgttttaag cgcttatatt atttgaacaa tcagccttat atttattttg ttcattatct
     7501 tccacaagaa atgatgcaat tcagcagaga agaatttggc aaagaatctt tgtatcgttt
     7561 gctgacgaaa aaaggatata ctattgactc attttctgat gatttctcgg cagtattttt
     7621 aacagaagaa gaacaaggaa ttctgaaaac agacgcacag atcggcatca aacggatacg
     7681 gaaatcgtct acgcaaattg gggaaatgat tgaatattcg gaggcggtct accatacgga
     7741 gctgcatcct taccatattg attatgaaac ataaataaaa aaagaggttg tgaaaaagct
     7801 gccctgcatc aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcgtttgg
     7861 ttaggcttat taccgcagat acacggctgt ttctggatta ccatttattc tggataaggg
     7921 ggtgtggcac gattttcaga aggaatctat gaaccaatca ctgtattgat tttagcttcg
     7981 atggtaaccc cattcggctt gacgatcgct tatttcttag ggaaaatcat tcgtaaaaat
     8041 atactcaatc gtcaagaaat cgatacattg aagaccgctt ttcctatggg gatctgtcag
     8101 atcacagagg gatgtttccc tatcgtattg aacgacttgg cacgcaatgt catagcaacg
     8161 ggagtcggtg ggacagtagg tggcggatta agcatgtttt ggggcgcaga tagccgtatt
     8221 ccggcatcgg gaatgtttgc agtcgctacg atgaccagac catgggcatt cattggtgca
     8281 ttgttagctg gttcttttgt cacaggaata accattttat tattgaaaaa accagtagat
     8341 ccgaatgcag aaatcatcca agaagaaaaa gaggaagaag acatatcttg ggatgatttg
     8401 acaattagtt aggagaggca gaatggatta caagaaaaaa gtacaataga tgtaccttga
     8461 gacaggaatg gtatgaaaga atcagagtta gaaagaatcg aatatacaga ttttggttta
     8521 ggagcgatcg aaactgtcca gagcatcggc accctccttg aaatgacgat gaaggaaaaa
     8581 tgagaacctt tcgttgtcga aagggtctgg tataccttat gtcgaaggaa aggaaacaaa
     8641 aaaactttcc tgtactattc caaaaaggaa agaaagttat tatacagtaa aaaaagaaat
     8701 tattcttcat gctagagatc agtacacgtt cgagcccaat atcaaacact agttccaggc
     8761 aggcgcaaca ggtgcggtta tcactgaatt ttcttccagc agtgacgatt caagtgatat
     8821 ttttacaaat ccagctattt atcgttaaca aaaagatttt taggagaagc tgatgaacaa
     8881 aaatgatcaa attttgatcg tttagttcgt cggctttttt atttagagaa acatcattta
     8941 tcagtgaaac ataacacgag aacaaaatga ttttttcgtt aataaaagaa acaagtattt
     9001 gttgtttaaa ataatacacg ttattggcat ataggacgaa atcccagtat cttctataag
     9061 taagtcattt catatcgatg aaaacacgtt atttattttt gttttatcta taaaatggct
     9121 gaaaaatgat
//
LOCUS       DAYYME010000109         9063 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000109 DAYYME010000000
VERSION     DAYYME010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.110"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..684)
                     /locus_tag="ACHBFW_002213"
     CDS             complement(<1..684)
                     /locus_tag="ACHBFW_002213"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318485.1"
                     /translation="MSENKKKWLLIHPFDYISRTNILEEILTENSIEYFNVVSDYSHI
                     RKQFLSSKERHMIKNRHVIKTIGYKKNISVMRILSHIWFSIKTLLFIRKEKPEIIYCR
                     IPPNTLVFFIAKFSRCNRVIFDVYDLWPESMPGIGNKYKFFTDLWKKLRDQYLSNSSL
                     VITECDYFKKFIKISGESKNMTIYPCSFNVDDQNDYGEKIVNKSLESTLNLAYLGTIN
                     NIVDLEAINE"
     gene            complement(677..1306)
                     /locus_tag="ACHBFW_002214"
     CDS             complement(677..1306)
                     /locus_tag="ACHBFW_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM9318486.1"
                     /translation="MSNRGIYEKFFKRLIDILFSILGLIMLWPLMTFVAILVRIKLGS
                     PVIFKQQRPGKHEKIFLMYKFRTMTDQRDEEGNLLSDDIRLTQFGKILRSTSLDELPE
                     LWNILKGDMSIIGPRPLLVEYLSLYSNEQRKRHFVRPGLTGLAQVSGRNAISWKEKFE
                     KDIEYVENISMMKDIKIFFLTLKKVIVRDGITSVASGTTEKFEGAKIDE"
     gene            complement(1299..2417)
                     /locus_tag="ACHBFW_002215"
     CDS             complement(1299..2417)
                     /locus_tag="ACHBFW_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM9318487.1"
                     /translation="MENKRILLASPHMSDEGYEQEYVKEAFDTNWVAPLGMNVNGFEE
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPIIYQGAIPV
                     FVDSDYETWNMNPEHLRTALEKYPNAKAVLVVHLYGLSAELDEITKICKEHNVVLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITVKGINTNHLIDELDKNNIEARPIWKPMHLQPVFDKYEYIGT
                     DVAQTLFEKGICLPSDSKMTDDDLYRVTNTIKRVMQNV"
     gene            complement(2419..3051)
                     /locus_tag="ACHBFW_002216"
     CDS             complement(2419..3051)
                     /locus_tag="ACHBFW_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM9318488.1"
                     /translation="MKAIIVGAGGFGKEIAFLLESFSRYELIGFVDDSLKMQNQELVG
                     KTVLGTIDSLLELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVASSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSIFPSVNISGHVLLGD
                     KNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNKTKNVGVPTKVIERWD"
     gene            complement(3062..4879)
                     /locus_tag="ACHBFW_002217"
     CDS             complement(3062..4879)
                     /locus_tag="ACHBFW_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFM9318489.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASAASSIIILLFINKQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVIGFVDDDPNKRNTYLSGKKVLGSLKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSMEELASGKISVSKLKTIDVVELLGRDEVELDIESI
                     KDQITNKVILVTGAGGSIGSEICRQIIQFDPAKLLLLGHGENSIYLIDRELRTHHQKC
                     PTEIVPIIADIQDRKKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPVTVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSMQTVMEFVKSLPQDDEQLAKDIVTFANASNK"
     gene            complement(4892..5656)
                     /locus_tag="ACHBFW_002218"
     CDS             complement(4892..5656)
                     /locus_tag="ACHBFW_002218"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFM9318490.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLQLGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVAQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(5684..6382)
                     /locus_tag="ACHBFW_002219"
     CDS             complement(5684..6382)
                     /locus_tag="ACHBFW_002219"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFM9318491.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSRDAGYYYGN"
     gene            complement(6394..7173)
                     /locus_tag="ACHBFW_002220"
     CDS             complement(6394..7173)
                     /locus_tag="ACHBFW_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFM9318492.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(7189..8133)
                     /locus_tag="ACHBFW_002221"
     CDS             complement(7189..8133)
                     /locus_tag="ACHBFW_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM9318493.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(8179..8958)
                     /locus_tag="ACHBFW_002222"
     CDS             complement(8179..8958)
                     /locus_tag="ACHBFW_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318494.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         2475 a         1855 c         1517 g         3216 t
ORIGIN      
        1 ttcattaatc gcttctaaat ctacgatatt attaatcgta cctaaataag caaggttcaa
       61 cgtagactct aagcttttat tgactatttt ctctccataa tcattttggt catctacatt
      121 gaaagaacat ggataaatgg tcatattttt tgattctccg gaaattttta tgaatttttt
      181 gaaataatcg cattctgtta taactagact agaattactg agatattgat ctcgtagctt
      241 tttccataaa tcagtaaaaa atttatattt gttccctatt cccggcattg actctggcca
      301 taaatcatac acatcaaaaa taactcgatt gcatctacta aacttagcaa taaaaaaaac
      361 taaggtatta ggtggtattc tacaataaat aatttcaggc ttttcttttc gaataaataa
      421 taatgttttt attgaaaacc atatatgaga taaaattcgc attacggata tatttttttt
      481 atatccgatc gtttttatca catgacgatt tttaatcata tgcctttctt tactggatag
      541 aaattgtttt cttatatgtg agtaatcaga tacaacatta aaatattcga tactgttttc
      601 cgtcagaatt tcctctaata tattcgttcg actgatataa tcaaaaggat gaataagcag
      661 ccactttttt ttattctcac tcatctatct tcgcaccctc aaatttctca gtagtcccac
      721 ttgctactga tgttattcca tcacggacaa taactttctt tagcgttaaa aaaaatattt
      781 ttatgtcttt catcattgaa atgttctcca catattcaat atctttttca aatttttctt
      841 tccaactaat tgcattacga ccactgactt gagctaatcc ggttaaacct gggcgcacaa
      901 aatgtctttt tcgttgttca ttactgtata gtgaaagata ctcaactaac agaggacgcg
      961 gtccaataat actcatatct ccttttaaaa tattccataa ttctggtagt tcatcaagac
     1021 tagtcgaacg caatatttta ccaaattgag tcaatctgat atcgtctgat aataaattac
     1081 cttcttcatc tctttgatct gtcattgttc gaaatttata catcagaaaa atcttttcat
     1141 gttttccagg tcgttgttgt ttaaagatca ctggcgatcc aagctttatt cgaactagga
     1201 ttgcaacaaa cgtcatcagt ggccataaca ttattaaacc taaaattgaa aaaagaatat
     1261 ctatcaaacg tttaaagaac ttttcgtaga tacctctatt agacattttg catcactcgc
     1321 ttaattgtat tagttactcg atacaaatca tcatcggtca ttttagaatc tgaaggtaaa
     1381 cagatacctt tttcaaacaa tgtctgagca acatctgtac caatatactc atacttatcg
     1441 aaaactggct gtaaatgcat tggtttccat atcgggcggg cttctatatt atttttatct
     1501 aactcatcaa taagatgatt cgtatttatt ccctttactg ttatgacact taaccaataa
     1561 tttggttcat tccaattatt ttctggcatg aattgtatat ttgacaattt agacaattct
     1621 tttttataaa aactatgaat atatcgtttt ttctcaattc tttcatttaa aactttcaat
     1681 tgtcctcgac cgatacctgc aacgatattg ctcattcgat aattatagcc aatttcattg
     1741 tgttgataat ggcgtgcctt ctcacgagat tgcgtagccc aaaacctcac tttttccacc
     1801 ttttctttgt cattagacac aagcattccg ccacctgaag tcgtgattat cttatttcca
     1861 ttaaaagaat aaattccata gtcgccgaat gtaccagtat attttccttt atatgttgtt
     1921 cctaatgatt ctgcagcatc ttcaattaaa acaacattat gttctttgca tattttagta
     1981 atttcatcta gttcagctga taaaccgtac aaatgcacta ctaatacagc ttttgcgtta
     2041 ggatattttt ctagcgctgt tcgcaaatgt tcagggttca tattccaagt ttcgtaatca
     2101 ctatcaacaa atactggaat agctccttga tagatgattg gattggcagt agctgagaac
     2161 gtcaaagact gacaaatcac tatatcatct ttaccgaccc cagccgcttt taaagctaga
     2221 tgtatagctg ctgttccaga agatagtgca gctgcatgac tagcaccgac atattctgct
     2281 aattcctctt caaaaccatt gacattcata cccaaaggag ccacccaatt tgtatcaaat
     2341 gcttctttta catactcttg ttcatatcct tcatcactca tatgaggaga tgctaataat
     2401 attcgtttat tttccaactt aatcccatct ttctatgact ttggtaggta cgccaacatt
     2461 tttagtctta ttattaatat tttttattac tacacttccc gcacctataa tgttcatatt
     2521 tccaattctt agattttgta ttattttagt acctactcca atttcatttt tatctcctaa
     2581 tagaacatgt cctgaaatat ttacacttgg aaaaattgaa ttataattac cgatttgagt
     2641 atcatgtccg atagtagagc cgatattgat catattgaaa tttcctaaaa ctacgtcagc
     2701 agtgtaagtc gtatagggca tcaaaatatt gccaattcct aactgaacat ttatgcccac
     2761 tagtgcactt gatgcaacta aattagggaa aatcaattta ttatttttat ttatcttttg
     2821 atatattttc tctttgatat ctggcgaagc gattcccaga aaaatagcag tttcttcttc
     2881 taattctaat aacgagtcta ttgttcccaa tacagttttt cccactaatt cttgattttg
     2941 catttttaac gaatcatcga caaaaccaat caattcatat ctagagaaac tttctagtag
     3001 aaaagcaatc tctttgccaa atccaccagc tcctacaata attgctttca ctttactccc
     3061 cctacttatt cgaagcattc gcaaaagtga caatgtcttt agccaattgt tcatcgtctt
     3121 gcggcaggct cttgacaaat tccatcactg tttgcatgga ataacctttg atgtttccca
     3181 caaagatctt ctcgtaaact gcttcatcgt ttctttcctt atctaacaat agttcttcat
     3241 atagtttttc ccctggacgg atacctgttt caatgatttc gatctcgtct tcgctatatc
     3301 cgctcaatcg gatcatgttt tttgctagat ctacgatttt cactggttca ctcatatcta
     3361 aaataaagat ttcgccgcct tttgctaacg ccccagattg aataactaaa cgacttgctt
     3421 caggaatcgt catgaaatat cgggtcatac gaaagtctgt tacagtgact ggccctcctt
     3481 gagcaatttg ttcccggaat actggaatca cactaccgcg agaaccaagg acattcccaa
     3541 atctgacagc tgagaatttc gtacatcctt cttcattcaa cccagtcacg atcatttcag
     3601 cgattcgttt tgttgaaccc atgacgtttg gcggattgtt ggctttatct gtcgagacca
     3661 tgacgaagtt ttttactttc gctgctttgg ctgcttctgc tacatttttt gtaccgaaaa
     3721 tattgttttt gactgcttct tttgggttat attccatcaa tggtacatgc ttatgagcgg
     3781 ctgcatggta gacgatatct gggtggtatt gttccatgat ctcattgatt ttcttgcgat
     3841 cttggatatc cgcaatgatc ggcacgattt ctgtaggaca cttttgatga tgcgtgcgta
     3901 attcacggtc aatcaagtaa atcgagttct ccccatgacc taaaagtaat agttttgcag
     3961 ggtcaaattg gatgatttgc cggcagattt ctgaaccgat tgatcctcct gcaccggtga
     4021 caagaatgac tttgtttgtg atctggtctt tgatggattc gatatccaac tctacctcat
     4081 ctcttcccag taattccact acgtcgatgg tttttaattt tgatacgctg attttccctg
     4141 aggcaagttc ttccatagaa ggcatcgtat tgactttcac atgagcagat tcgactaact
     4201 cgaagattct tcgtaatttt tttcttgata aagaagggat agcgatcgtt accatttgga
     4261 tgtcatattt ttcgatcaat tccggtatat cttttaatga ccccagtact ttttttcctg
     4321 aaagataggt atttctttta ttcggatcat catccacgaa tccaatgaca tggatatctt
     4381 gagccgtttt agaaccaaga aagctgttgt ataaaatacg tccaccttcg ccggccccaa
     4441 cgatcaaggt atttttagcg gaatctgttg atagatacct catatttttt gtttcgatat
     4501 agatacgcca gatcaatcgg ctgccaatga tcagtaatag cgaaagtagg tatgcaaaga
     4561 tcactaaacg taaactgtat tgtttattta tgaaaagcaa tatgatgata cttgaggccg
     4621 cacttgcaga taacgaacca aaaatcgcaa tgatctctct taaatttgta taacgattga
     4681 ttcgagtaaa aactttgaat aaactaccat agaataaata aaaaccaatc gacaaaccga
     4741 tagagattaa aataaggtcc attggtatag ctacaaacgg cttcatgaat ttagctgctg
     4801 caatattcgc agcaatcaac aacaagctgt ccacaaatac caatgtggca atttttcttc
     4861 ttcttgttaa tacaaacacc ctacattccc cctaaaataa accaaacttc ttctttttta
     4921 cctcttcata gactggataa ctgatctcat ccccgttgat caggtccttt gcgacttgtt
     4981 tcatctgtgc tactttttcc ttaccaaagt ccttggcgat ttgctcataa cattctttta
     5041 agtaaaaggt acgtttagag accccatggg catcggaagc agccatttgc acgaggttat
     5101 gtttgaccat cattttggct gttttctgga tgtcttttcc aaatacaccg acataacttg
     5161 gtgcggtgag ttgagccaag catcccatat ctaggaatgg cagcaaatga tttgggtctt
     5221 ctcgaaaatg cgcgttgcgt tctggatgga cgatcactgg tgtctttcct agttgtaaca
     5281 actcaaaaaa gagccattct gcatataacg gcacttccag tgtcggaaat tcgattagaa
     5341 gatatgtatc atctaaatct gtaaataaaa tttcatctcg gtggatgtct tcaatcagtg
     5401 ttccggtaat acgtacttct tgtccttcca ataacgtcaa aggcaattgt cttttctcta
     5461 attctgcttg taacgaagcg actagagaaa tgacttgtga tttttcattg ctgtattttc
     5521 cattattgtg atggggtgtg cataaaatat gtgtgatccc ttgttgaatc gctttttcag
     5581 ccatggcgat actggcatct atgttttctg ctccgtcatc gattcctggc aggatatggc
     5641 aatgtagatc gatcatttct cttgtctcct ctctttttat ttcttaattc ccgtagtaat
     5701 agccagcatc tctgctgtgt tctgctccat tgtatacaac accaaggact cgcgcttgta
     5761 ccatatctaa cagttctttt gctttagtca atgattcttt tcgtgacgta ttttcccgta
     5821 cgaccaataa ggtgccatcc gtttttgaag acatgatctg tgcatctgtt acggcaacga
     5881 ctggtggcat gtcgaaaatc actacatcaa atagttgtct tgcttctgct aaaatctgat
     5941 ccattcttgg cgaacttagt agttctgatg gatttggtgg tttaggtcca cttggcagaa
     6001 tcgacaaatt gtcgactggt gttcgttgga tctcatctgc tacacttcct gatgaactaa
     6061 gaacagtact taatccgctt gcgttgttca acttgaacgt tttatagatg acgggcttac
     6121 gtaagtctgc atcgactagc agtacccgtt gaccagattt ggcaaaaaca acggcaatgt
     6181 ttgctgcagt ggtggatttt ccttctccag gacctgatga agtcacgaca atcgttttga
     6241 tttgctgacc agctgcggag gcaaactgga tattcgtccg gatcgtacga tattgttcgg
     6301 cgattggtga agatgggttt gttaatgtga tcaaactgac tgcatgagtt tgagttgctt
     6361 tttgtttctg tgttcgtgcc atttttcttc ctcctagact cttgagcggc ttctgcgtga
     6421 tggggctgtt ttatcgtttg tagcttttgg tcgtgcagga gctgatcctt ttttacggat
     6481 accagcgttc aattcttttg ctgacatttc tggaactgtt cctaagattg ggaatcctaa
     6541 tgtatccgtc acatattttt catctttcac tgtccgatca agtaattcga ataagaatgc
     6601 cagacctacg cctaccatca ttcctaacac taagccaatc actagattca atttattatt
     6661 tggcgaaaca ggtgtcgtat tggcaactgc acctgaaata atcgagattt tatcgacatt
     6721 catcacgtct ttggcgtttt cttggaaaac ttgtgaagtt gtattagcga tctgttgagc
     6781 agtcactgca ttcgtgctta ttgcctggat cgagaacatt tgtgaatttt gtgactgatt
     6841 gacggagatc gcatctttga tttgtccagc agtcattttc acattgtctt ctgtttctaa
     6901 acgatctttg acttcattta gtactaaatc actgacgatc atatctttat acgtattgat
     6961 catttgcagg ttcgtattga catcattgac atttgctgtg tctgattgtg gcagtgtcac
     7021 gatcaattgt gcttgtgaac tgtattttgg tgtcaagatg aaaaatgtta cgattcctga
     7081 gatggccaag ccagcaaaca ttgagatgat gattgttacc aagtgtttgc gtaatgtttc
     7141 aaaaatttct ttcaagctga ttgtctcttc cattttttct ccactttcct atttattctc
     7201 tagattcatt tgatctttta attctttttt cacacgatca agttcttgat cgctgacttt
     7261 ttgataggag atattatctt gcatgaagcc ttctccttgc agctgatctt gcttgatcgt
     7321 accaaatgcc tcccgataat ctaaagcgat ttttttcatt tcatcaaagc tcatgtctgt
     7381 tttcatattc gactctagag cattcaaaac ggattgatag ttgctgacac cgtttaaact
     7441 caatactttt tttgctacac cttctacgat ctttctttga cgctcttgtc taccatagtc
     7501 accatttgga tcttcataac gcatacgaga ataagataat gcctgatccc cgttcaatgt
     7561 aattttaccg attggaaagt catagccatc ttgtgagaaa gtgaggtcat tattgacttc
     7621 gataccacca actgcatcaa cgagttcctt tagccctttc atattgatag agacatagtg
     7681 atcgattggg atgtctacat atttttgtag ggtatccatc gccatggcag ctccgccgaa
     7741 tgcgtaagcg tggttgatct tgtcttttgt gtcacgacct acgatgtcga catacgtatc
     7801 tcgaggaatg ctgacgatcg ttgtctgttt gtcttcagga ctgactgtgg caaccatcat
     7861 cgtatctgat cttccttgat cgacccgacc taaatctcca gtatcaatgc ccatcaatag
     7921 aacagagaaa gattcttgtt tgtccagatc aactggtttc gttcgttctc cttgggttcg
     7981 ttccactgat tcgtaagttt tctcaataga atgtgagaga tccagatata attttgctcc
     8041 tactcctaca attgctaata ctaatacgat aagaactgct aaaataatca gaaatatttt
     8101 tttccctctt gattttcttc ttcgtcttgc catagtttcc tccgtttgct aaccttgctt
     8161 gaatcaacgt tttaactgtc aataacttat attgctatag cccatttgct gagcaacagt
     8221 ttgtgctttt gttgcgtatg ctgcgcttat tttttcattc cggatctgct ctatttgact
     8281 gagcgccgtc aaatattgat cgtaagtagc ggtggcagtc acttgaccat ccttcaacat
     8341 cgtatcgaat aactgatcta gttgcgtgac ttgcgcgatc tgagtatcag cgaatcctag
     8401 atattgcata acaaccgttt tccaaggact atctttaaaa aagctcatat tttcacgtac
     8461 acgagcgaca tctgcacttg tcaccttttc tttgatgacg acatcgtcta cagcttgctg
     8521 ccagtttgat acatttttag taaataaatc gttgacagct gtctggatct tcagttttga
     8581 ttctgcacta tctaactgtg tcacgattgc tttcttctca tcttggattg caacagcttc
     8641 tttaggcaat tctgattctt tgatgtcgta atcatctgct gaaagtttga cagaggatag
     8701 cttatttgac aaacttgtga ttgtttcttc tgttatttca gggtttagaa aatttttttc
     8761 ttttgtacta tacagcttgt ctacttcttt tgataaagaa gtcaaggtgt ctttttgatt
     8821 ggtgatggca gattctgcat aattgatccg ttgctgatgt tgccgctgat tattgatatg
     8881 gtaaccaaac aaacagatcg caatagcacc tgcacctacc gccacagcag tcaatgactt
     8941 gacgaatggc ttttccatag attcactcct aagtattgat gatagataaa ttaagatata
     9001 attcacatac ataaaaatga cagcttttta ttttaaaaaa cataattaaa ttaatatatt
     9061 cac
//
LOCUS       DAYYME010000110         9046 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000110 DAYYME010000000
VERSION     DAYYME010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.111"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            31..2640
                     /gene="clpB"
                     /locus_tag="ACHBFW_002223"
     CDS             31..2640
                     /gene="clpB"
                     /locus_tag="ACHBFW_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFM9318495.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2791..3477)
                     /locus_tag="ACHBFW_002224"
     CDS             complement(2791..3477)
                     /locus_tag="ACHBFW_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318496.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3455..3949)
                     /locus_tag="ACHBFW_002225"
     CDS             complement(3455..3949)
                     /locus_tag="ACHBFW_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM9318497.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(3969..5189)
                     /locus_tag="ACHBFW_002226"
     CDS             complement(3969..5189)
                     /locus_tag="ACHBFW_002226"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9318498.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5336..7171
                     /gene="lepA"
                     /locus_tag="ACHBFW_002227"
     CDS             5336..7171
                     /gene="lepA"
                     /locus_tag="ACHBFW_002227"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFM9318499.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            7367..8278
                     /locus_tag="ACHBFW_002228"
     CDS             7367..8278
                     /locus_tag="ACHBFW_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9318500.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
BASE COUNT         3064 a         1593 c         1836 g         2553 t
ORIGIN      
        1 tcttaccttt aattaggagg gtatacctgt atgaatattg agaaaatgac tacgacgctt
       61 caagaagcga ttgctgaagc tcaacagatt gcggtcactc gaaaacatca agatatcgac
      121 attgcccatg tatggaaaat ctttttacaa tctaatcatt ttggtcgaaa tttctatacg
      181 gatgccggat tggatgtcga atcttttgaa catgaaattg atcgtctact ggatgagtat
      241 ccagttgttt ctggtggaaa cgtccaatat ggacaaaacc tcagccagaa tttctttcga
      301 ttattgaatg aagcagacca aatacgtgaa agcttcggcg acgagtttct ttcaacggaa
      361 gtcgttattt tagcgttgat gaaattaaaa aattatccgt taactgttta tttgaataaa
      421 aacggattat ctgaaaaaga attgcgtaaa aacatcgaag agatgagagg aggagaaaga
      481 gtgacctcac aaaaccaaga agaacaatac aaagcattag aaaaatacgg cgttgattta
      541 gtacagcaag tcaaaagcgg aaaaatggat ccaatcatcg gtcgtgatga agaaatccgt
      601 gatgtcatcc gtattctttc acgaaaaaca aaaaataatc ctgttctgat aggagaaccc
      661 ggtgtaggga aaacggcaat cgttgaaggg cttgctcaac gtatcgtacg caaagatgtg
      721 cctgaaaatt taaaagataa aacgattttt tcattagata tgggtgcatt gatcgctgga
      781 gcaaaattcc gtggtgaatt tgaagaaaga ctaaaagcag tcttgaaaga agtcaaaaaa
      841 agtgatggac gtatcctctt gttcatcgat gaaatccaca atatcgtagg ggcaggaaaa
      901 acagaaggaa gtatggatgc cggaaatctg ttgaaaccaa tgcttgctcg cggtgaatta
      961 catttgatcg gtgccacaac tttagatgag taccgtcaat atatggaaaa agacaaagcg
     1021 ttggaacgtc gctttcaaaa ggtattagtc aaagaaccaa cagtggaaga tacgatcagt
     1081 attctgcgtg gattaaaaga acgttttgag atccaccatg gtgttaacat ccatgacaac
     1141 gcattagtag cagcagctac tttatctgac cgttatatta ctgatcggtt cttaccggac
     1201 aaagcaatcg atttgatcga tgaagccagt gcaacgattc gggtagaaat gaactctatg
     1261 ccaacggaat tagatcaagt tactagacgt ttgatgcagt tggaaatcga agaagctgca
     1321 ctgaaaaaag aatcagatga tgcaagtaaa aaacgcttga aaaatttgca agaagaatta
     1381 gctgaacttc gtgaagaagc aaatgcgatg aagatgcagt gggaaacaga aaaagaagaa
     1441 gtcaattctg tttcagcaaa acgtgctgaa atcgataaag caaaacacga attagaagat
     1501 gcagaaaata actatgattt agaacgcgct gctgtattgc gtcatggtac gatacctcaa
     1561 ttagaaaaag aattgaagga attagaagct aaagcaaaag acagcgagat taagatggtt
     1621 caagagtccg ttaccgaaaa tgagattgct caagtcgttg gcagattaac gggtatcccc
     1681 gtaacgaaac ttgtagaagg tgaacgagaa aaactaatta aattgaatga aacacttcat
     1741 aaacgagtga ttggtcaaga tgaagcagtt gatgcggtca gtgacgcggt aattcgttca
     1801 cgggctggat tgcaagaccc aaatcgtcct ctaggttctt tccttttcct aggaccaact
     1861 ggtgtcggaa aaaccgaatt agcgaaagcg ttagctgaga acttgtttga ctcagaagat
     1921 catatggtac ggatcgatat gagtgaatat atggaaaaac atgctgtgtc tcgtctagtc
     1981 ggagctcctc caggctatgt aggttacgaa gaaggtggtc aattaacgga agctgtcaga
     2041 cgaaatcctt atacaatcgt cttgttggat gaaatcgaaa aagcgcatcc cgatgtcttc
     2101 aatatcttgt tacaagtcct agatgacgga cgcttgactg attcaaaagg gcgagtagta
     2161 gatttcaaaa atacggttct gatcatgacc agcaatatcg gttcacagtt actgctggaa
     2221 ggcgtgactg ctgatggaac gattccagaa gcagtggctg aacaagtcaa cacgctatta
     2281 cgtggaaact tcaaaccaga attcttgaac cgaatcgacg acacgatttt atttacacca
     2341 ttgagcctag acaacgtgaa aggaatcgtt gacaaaatgg tggcgcaatt ggcacaacgt
     2401 ctggaacatc aagaaatcct gctgacaatc agtgatgaag ccaaaacgtg gattgctgaa
     2461 aatgcttatg aaccagcata tggtgcaaga ccattgaaac gatttatcac taaggaagta
     2521 gaaacaccac tagctaaaga aatcgttgca ggacatgtta tgcctaaatc aaaagtaacg
     2581 attactttac ttgatggaca acttcatttc aaaacagaag agctagaaga aatcgtctaa
     2641 tcttccattg agactataaa agaaaaaagg ggtctgacac aactagtgtc agacctcctt
     2701 ttttcaaata aacagtgttc cagaagtagt ttcttcgaaa aataagccga aatttcttga
     2761 agctgcccgc ttctgtcaca acctctttta ttaggtattt ttcacttttt ctaatttata
     2821 actttcaaag taacgctcat cttttttact ttgagaccat gcaaacaatc ccattaaggc
     2881 agcacctatc gtatttgtca gcaagtcgcc catcgtatcc atcaatgctg ctcgtccgac
     2941 aaacagtgtg ccatcagaag cagcaaaacg ttgaagattc atgcctaaga actggtcgca
     3001 taagaattcc cagaactccc aaaacacgcc gcaaagtcca gcaaacgcaa agccgaataa
     3061 gaggaacaac caaggactgg tttttgatat ctcagcatct ttcatcaagt aaccaatcaa
     3121 tccatagcca agagctgtca ggaccattgg actcaccgca tgtaagattt tgtcccaaaa
     3181 agagatgata ctgatcaaat gcatccctgt gcctaaaaag acagagatga acaagaaaaa
     3241 ccagtaaaac aaaacgattg ctgctggtat tttaagtttg aaaactttca aaatgacttg
     3301 tggcatgaaa atcaatgcaa tccctaataa agattccacg ataaatgtga ttccttgaga
     3361 agtcgaatat ttttgtgtga taaattcata aaaattatag cagacaacga atacaccaaa
     3421 agcaagtaaa gctatttgca cctttttgat ttctttaatc actgaaagtc cctccatcta
     3481 aaaatctttt tacttctgaa agcaactcaa tttctgaaga aaccactttt ccgttcaatt
     3541 tgatcaaacc tattgtatat aagttcaggt agtgaaattg attttcagct gtttcttgta
     3601 gtgcagcgat ttttttcggg tgggttccgc cttgctgtcg agtatcagta tataagccga
     3661 tcactgggat gcctttcgca taagccacac cgatttccga tgctaccccg gcatcgatcg
     3721 tcagcccatc tagtaaagca atcattaaat cactagccaa cactttttct gtgtcagcaa
     3781 gagcaatcat tcgactgtct gcataagcag ttttgtcatt gattgcttca ttttcttgag
     3841 gaagatagat cgaaagctca gaagagagct ctctgatttc tttaactaac tgttggttat
     3901 ataacaaatc acttttagca aacattggtg cggcaaaata aattttcatc taaacatcat
     3961 cctttttatc aaaaataatc gctaaagtaa gcttccatgt ttggaatgat acgctctagt
     4021 gacttcagtg tttcttcatc tgtttctaac cgttcgattc ttgccaaata agaagcacgg
     4081 cgataattca tcatcaagca agtcaatgtt tggatatcca gtcgaacgga ttttcctata
     4141 ggttcattta agacacggac ttgtccctgt tcatcccatg ttaaagcgaa gacaccatta
     4201 ttccaaggag cgacaggatc ttcaatgatg aaatgaaaag catcagctgt tccaacaaac
     4261 ggaaaccgtt gaaggaattc ttttacatca acgatccgag ccataaaata tggttcgatc
     4321 tgctctttga tctgactatc ttcaatcaaa aatgctaaag gctcattttt ataaatatct
     4381 ccatgtaccc agtagatcat ggaaaaatgt gcggaaataa agttccataa gccatttcga
     4441 gcttcttgat caagatagaa catttctttt acgtgaaata tttcttcagc tacccagtag
     4501 aacagaacac ctcttggttc atggttgcta tcgtagtaga cagcggctgt gcgctcatct
     4561 tcattttcga aacgccaata ctcttcccaa tgaaaatcac tgcggaataa agcaccatgg
     4621 ttttgttggg caaatttgtc ataaacggaa aatacgtctg gatgatcaac tggtaatcgc
     4681 tcaaccatac cacttactgg gatttgttta ggtagctgtg tatctctgat tttaaaggaa
     4741 agtttatcag acatgatttc ccatccttta cgccgatagt aggggatact gtaaggatac
     4801 aaataagaga tccattgctt attcttgcgc atattatcca atgcaacgat aattagatct
     4861 tgcatcaatc catggttagc atattctgga taagttccaa ctccagtcac accacccatt
     4921 ttataacgtg caccgtgaat attgacttca caaggataga tagcgatttg agagatcagt
     4981 ttattttcgt ggaaccatcc gaacactttc gatacttcca aaatcggttt tttggaacgg
     5041 ataaaagctc gtttattctc aaacccgctt tcctcgatgt ctgcttcagt cacttggaac
     5101 acataggaaa gcaattcatt gaattgatcg atatgttctt gagtgaccgg ttttaattca
     5161 agattttctc taaaatcttt ttgatccatt ttttcatctc catttattag cttgataaca
     5221 gtatagcaaa attctacaaa aaaaacttct atagctgact aattaaaaac tctttaaatt
     5281 actgcttttc gttggtataa tggaaagtgc tgattatatg aaaagaggag acgctatgga
     5341 tataaagaaa atgaaagaac gccaagagca gattcgtaat ttttcgatca tcgctcatat
     5401 cgaccatggg aaatctacat tggccgatcg tattttagaa atgacgcaca ctgtgacgtc
     5461 tcgcgaaatg caagatcagc tgttagactc gatggatttg gaacgtgaac gtggaatcac
     5521 gattaaatta aatgctgtag aactcaatta cacagcgaaa aatggagaaa catatatctt
     5581 tcacttgatt gacaccccag ggcacgtcga cttcacctat gaagtctctc gaagccttgc
     5641 tgcctgtgag ggtgccgttt tagtggtcga tgcagcacaa ggaatcgaag cacaaacatt
     5701 ggccaatgtt tatttagcgt tagacaatga tttggagatc ctgccagtca tcaataaaat
     5761 cgacctaccc gcagcagatc ccgaacgtgt ccgtcaagaa atcgaagacg taatcggaat
     5821 cgatgctagt gaagctgtat tagccagtgc caaatcaggg attgggattg aagatattct
     5881 cgagcagatc gtggaatatg ttcctgcacc aagtggagac ttagatgcac cgctaaaagc
     5941 tttgatcttt gactctattt atgatagtta ccgcggtgtt gttctaaatg tccggatcac
     6001 ggacggtatg gtcaaacccg gagacaaaat caaactgatg agtaatggca aaacatttga
     6061 tgttacagag gtcggggtct tctcgccaaa agcagttgct agagactttt tgatggtcgg
     6121 tgatgtcggt tatatcacag caagtatcaa aactgtccaa gatacacgag taggggatac
     6181 agtaacgtta gcagacaatc ctgccgctga agctttacca ggttaccgaa aaatgaatcc
     6241 gatggtttat tgtggtcttt atccaattga tacttctcgt tacaacgatt tgcgggaagc
     6301 tttagaaaaa ctgcaattga atgatgctgc tttacaattc gaaccagaaa cctctcaagc
     6361 attaggtttt gggttccgct gtggattcct cggacttctt catatggatg tcgtccaaga
     6421 acgcttggaa cgggaattca atctagaatt gatcacgact gcaccatccg ttatttacca
     6481 tgttaataaa acggatggta cgactgctac agtagataac ccggcagatt ttcctgaacc
     6541 tgtaacgatt caggatgtag aagaaccatt cgtcaaagca cagatcatgg tcccaaatga
     6601 ttttgtcggt gcagtaatgg aactttctca acgaaaacgg ggagaattca ttacgatgga
     6661 ttatttagat gattaccgag taaatgtcgt ctataatatt ccgctttctg aaattgtctt
     6721 tgactttttc gacaaattga aatcaagcac gaaaggctat gcctcattgg attatgaaat
     6781 gtctggttat caaaaaagca agttagtgaa gatggacatc ttgctgaatg gagaaaaagt
     6841 cgatgcatta agctttatcg ttcaccgaga ctttgcctat gaacgtggaa aagcaatcgt
     6901 tgaaaaactg aaaaaactaa tcccacgcca acaatttgag gtgccaatcc aagcagctat
     6961 tggacaaaaa atcgttgccc gttcagacat caaagccttg cgtaaaaacg tcttggctaa
     7021 atgttatggt ggagatgttt ctcgtaaacg taaactctta gagaaacaaa aagaagggaa
     7081 gaagcggatg aaacaaatcg gctccgtcga agttccgcaa gaagccttca tggccgttct
     7141 gaaaatggat gagcaagata gtgtgaaata acttataatc aatcgaaaaa cctattaaat
     7201 aaaccttttt gagtagatgc aacactgagg attatctgta taaaagattt taaactgtca
     7261 tttggttatc acggcgatac taaaattaac aaaattggaa tatttttctt gaagattttt
     7321 agctgtcagt atatgatgaa agcatctcat aggaggtgct tttttcatgg ttgataattc
     7381 aagtggaagg gttcttaaat ctctaagaaa agagaaaaaa ctatctcaaa aaaagttagc
     7441 tgatttagca ggtatttcac aaagtacttt agttaaatat gaaaaagggt caagaaaaat
     7501 ccctaaagac gtagataata ctctaagcaa gattctgaat atagaaactc taataaaaga
     7561 tgaggaagat aagattgaga ttctcatcgg taaactaatt gcatacagag atatgaataa
     7621 gttacttaac aaagagttag ctgataatat aggaattagt gaagtattac tcagttatgt
     7681 gttaaataga aagagaaatc cgtcaaaaga aatgcaaaaa aaaatcgata tttttctatt
     7741 aagtaatgaa aaagaaattt tgaaggagat taatcgtgat agtgagatat ttagtttatc
     7801 aaaagacgat aaaattgtta tgggaaaaag aatacgagaa gtccgaaaga accgggaaga
     7861 gacgcttgaa aaatttggta aaaactttac catctataca ggcaaaaatg ttataagtag
     7921 atgggaaaaa ggtattaata ttccagatat tgaaaaacta atgaatattg cctatcttgg
     7981 taaggtcact gttccttacc ttatgtacgg agaagattat aagaatatac ttccaaaaga
     8041 tgaaagagtt agcgatttta aaaagataaa ttcttttagt atgggattaa ggatgaggaa
     8101 aattaggaaa gattattatc tagaaaggga ggagtttgga aagctttttt ctccttcaat
     8161 ttcaaaatgg tccatagata ggtatgagaa tggtagagat atacccaata ctaatcgaat
     8221 tatccaatat gcttatatag gaaatctttc tttagaattt ttgatttacg ggatataaaa
     8281 atagtttacg tcgatactta ttaagttatc gacgtttttt attatagaaa aaatttataa
     8341 aaggaggttt cttgatatga taagaaacaa aaagggtttg ttgaaagttg ctttactggc
     8401 agcttttttc taagtaactt tttgacaagc tcaatggcaa cacaagcgga agaagtaata
     8461 caagaactaa ctgttatgaa taacagctag ataaaatggg ctatgtatgt tatccaacaa
     8521 tttagtaatc actagaagcc tagcgaaagt tcgcttgata agttatagat gagtattgat
     8581 ttgtagtgtt tccaaatcaa aagggtacag agccacatga aatatgttgt cctctgcagc
     8641 gtcaggatgg gaatccatac catggagatt cggagatctg tgagggaata cttacgtggt
     8701 taatatgtca gttgtgttgg acgtagtaat ggtcatagtg ggtagattga ctgaattctt
     8761 atatgagaaa atgaaaccaa caagaagata gggttattta tacagaattg gggatctttt
     8821 ctggaaaagt aatcggataa tgcgagattc gtacggtctt ttttaaattt ctctaaaaaa
     8881 aacggcatcg aatgggttgg gtgattgata agcaatgaat catagactga tatagaataa
     8941 aaaatgattc tatttatctt tttacatcta tcgttttttc aagtagccgc aaattatatc
     9001 agcattcaac aatttttgta tacaagttct agtaaaagga tctgaa
//
LOCUS       DAYYME010000111         9018 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000111 DAYYME010000000
VERSION     DAYYME010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.112"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1101
                     /locus_tag="ACHBFW_002229"
     CDS             <1..1101
                     /locus_tag="ACHBFW_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9318501.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1615)
                     /locus_tag="ACHBFW_002230"
     CDS             complement(1280..1615)
                     /locus_tag="ACHBFW_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM9318502.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1599..1862)
                     /locus_tag="ACHBFW_002231"
     CDS             complement(1599..1862)
                     /locus_tag="ACHBFW_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM9318503.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="ACHBFW_002232"
     CDS             complement(2322..3125)
                     /locus_tag="ACHBFW_002232"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9318504.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="ACHBFW_002233"
     CDS             complement(3103..4227)
                     /locus_tag="ACHBFW_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9318505.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="ACHBFW_002234"
     CDS             complement(4243..5058)
                     /locus_tag="ACHBFW_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9318506.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="ACHBFW_002235"
     CDS             complement(5048..5920)
                     /locus_tag="ACHBFW_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9318507.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="ACHBFW_002236"
     CDS             complement(6005..7294)
                     /locus_tag="ACHBFW_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9318508.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7438..7950)
                     /locus_tag="ACHBFW_002237"
     CDS             complement(7438..7950)
                     /locus_tag="ACHBFW_002237"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318509.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8017..8160)
                     /locus_tag="ACHBFW_002238"
     CDS             complement(8017..8160)
                     /locus_tag="ACHBFW_002238"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM9318510.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
     gene            complement(8643..>9018)
                     /locus_tag="ACHBFW_002239"
     CDS             complement(8643..>9018)
                     /locus_tag="ACHBFW_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9318511.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVWLSVRTAFFVG"
BASE COUNT         2778 a         1933 c         1427 g         2880 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacagccaga ctgtagacaa aagctgttcc
     8701 aaactcgtaa ctcgagcttg gaacagcttt ttatctactt cgtttcgaat ttttgaacaa
     8761 aaaaataaag gctccttgcc tttccatagc caggtggcta atttctttaa attcattgca
     8821 gcaaacacta gcatcgtatg cgtcgcgact ttttccaagc ctcgatactt ggtccaacgc
     8881 atcccatgct tttcttttgc atccgcaaat attcgttcaa tcgtttgttt ccgttttttg
     8941 taaattgatt tattcagatc tgttaggcga agatgttcca cttcatccat cagatctccc
     9001 caaacatgtc tcgtgatg
//
LOCUS       DAYYME010000112         8944 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000112 DAYYME010000000
VERSION     DAYYME010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.113"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..1152)
                     /locus_tag="ACHBFW_002240"
     CDS             complement(13..1152)
                     /locus_tag="ACHBFW_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFM9318512.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACHBFW_002241"
     CDS             complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACHBFW_002241"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFM9318513.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACHBFW_002242"
     CDS             complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACHBFW_002242"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFM9318514.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACHBFW_002243"
     CDS             complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACHBFW_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFM9318515.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACHBFW_002244"
     CDS             complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACHBFW_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM9318516.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6166..7257)
                     /locus_tag="ACHBFW_002245"
     CDS             complement(6166..7257)
                     /locus_tag="ACHBFW_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFM9318517.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACHBFW_002246"
     CDS             complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACHBFW_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFM9318518.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8800..8871
                     /locus_tag="ACHBFW_002247"
     tRNA            8800..8871
                     /locus_tag="ACHBFW_002247"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8833..8835,aa:Arg,seq:ccg)
BASE COUNT         2320 a         1897 c         1648 g         3079 t
ORIGIN      
        1 attaaaaaaa tcttacgcta agtaggataa aataattcct tgatattttt cgatcatatc
       61 caaataatta tcgatttcaa catactcatc tacttgatga ggcaaagtaa cgacaccagg
      121 cccaaagacg acaaagtcga aggaatggtt agctttcgta aattcggctg catccgttgt
      181 tgctgccgct ccaactaaag gtaatggctg gctgaattgt tgctgaatgc aatgaatcaa
      241 aggagaatct ggatcagctt tgacaggtat tttgttgtaa tcgatcatca attccaaatg
      301 atagtctgtc tcttgattca attcattgac gattgattgc agcaatgcaa tgattttatc
      361 atttgggtat tctggaatcg atcgaatgtt cccttggagc tgcgcatggc ttgggatgct
      421 gttgacttga ttcccgccac tgatcaatgt aacattatgg attgtttttc ctaggacagg
      481 attctcgatt gtttcagcga ggtgattcat ttcagcattt gctttcgtaa tgaattcatt
      541 taggtgattg attgcattat aaccttggtc tggcatagag ctatgcgctt cttttccgtg
      601 cgaggtgact gtatagttga ttgatcccat gtgcgtatac atcaaactgt aattggttgg
      661 ctctccgatg atcaaggcat ctaagtcatc tacatagcct gctttcgtga gctgttcccc
      721 acctagttca cccacttctt ctccgacagt ggctaataat ttgactgttc cgttaaatgg
      781 ttttcctgac tctttcagtt cgatcatggc aatcaccatt gctgccaagc cactcttcat
      841 atctgtagca ccacgtccgt acaaacggtt cccatggatt tctgctgcaa agggtgcata
      901 ggtccaagac gattcatcac cagccgccac gacatccata tgcccagata ggcctaacac
      961 ttttcctgat tggccctttt ggtaacgagc aatcaaatta tctctaccat ctctgtaaga
     1021 aacaatctct cctgttatgt catgctttgc caaaagctta ttcaaatagg ctgctacttc
     1081 accttcgttt ccattcactg atttaattcg gataatctct tgtaaaatcg cgattttttc
     1141 ttcttttttc atctaatcct ctcccttgcc atttcttttt attgtatttg agtgtgctcg
     1201 aattttcaaa taggagcaac caaagtaaaa atgaagaaag agagccactt tatgaaaaat
     1261 atgctgctac ttccctccta ttttatttat acgagaggac ttgcccatag attcctattc
     1321 tactgttcta tccttaaaag ctcttatatg atgctagtta ttgttagctt tcctttttca
     1381 aggatgatct tttcttgtac cattctttgt aaacatcata tacagaagct agttgcaaaa
     1441 aaactctcta cctgctgata tttcagcaag tagagagttc gattacttaa ctaagtgatc
     1501 atactcgact atctttcaat agtcgatcat gtatcttcat tagaaaagaa ttatttgttt
     1561 tttaagttgt agaaagattt caaacctttg tattctgcaa cttcaccaag ttggtcttcg
     1621 atacgtagta attggttgta tttagcaata cggtcagtac gtgatagaga accagttttg
     1681 atttggccag cgtttgttgc aacagcgata tcagagattg ttgaatcttc tgtttcacca
     1741 gaacggtgag atacaactgc tgtgtagcca gcttctttag ccatttcgat agcttcgaaa
     1801 gtttctgtta atgtaccgat ttggttcact ttgataagga ttgagttagc aattcctttt
     1861 tcgatacctt cagataattt ttgagtgttt gttacgaaca agtcgtcacc aactaattga
     1921 actttgtcgc ctaatacgtc agttaatttc ttgaatccgt cccagtcgtt ttcgtctaat
     1981 ccatcttcga ttgagatgat tggatattta gaaactaatt cttcgtagaa tttgatcatt
     2041 tcgtcagttg ttttttcgcc ttcgcctgag tcagctagta cgtaaacacc tttttctttg
     2101 tcgtagaatt ctgaagaagc agcatccata gcaagaacaa cgtctttacc aggtacatag
     2161 ccagcttttt cgattgcttc gatgattact tcaaaacctt cttcgtttga accaaggttt
     2221 ggagcaaatc caccttcgtc accaacagaa gttgctaatc cgcgagcttt caagattgaa
     2281 gctaatgcgt ggaatacttc agcacccata cgtagagctt ctttgaatgt tggagcgcca
     2341 acaggcatga tcatgaattc ttggaagtcg attgagttat cagcatgtga tccgccgttg
     2401 atgatgttca tcattggtgt tggcaatact tttgtgttga atccgcctag gtagtggtat
     2461 aaaggtactt ctaggtagtc agcagctgca cgtgcaacag cgatagaaac acctaaaata
     2521 gcgttcgcac ctaatttacc tttgttagga gttccgtcta aagcgatcat agctttgtcg
     2581 atagccattt ggtcacgaac atcgtagcca atgattgctt cagcaatgat gttgtttacg
     2641 ttgtcgacag ctttagttac acctttaccg ccataacgag ctttgtctcc gtcacgtaat
     2701 tcaactgctt cgtattcacc agttgaagca cctgaaggaa ccattccgcg gccaaaagct
     2761 ccgctttctg tgtatacttc tacttcgatt gttgggttac cacgtgagtc taagatctcg
     2821 cgagcgtaaa catcagtaat aattgacatg ttttgtctct cctttgagtt tgttttttcc
     2881 taagggattt ccctcatata cgattttagt taattttgct cattagtgca aacatttctt
     2941 gcatttttcg caaattttca acaattattt tacagcgtct aacaatgaca agaatgaatc
     3001 tgcttgcaag cttgcgccac cgactaaagc accgtcaaca ttttcttttg ccatgtattc
     3061 agcaatattt tctggtttta cagaaccgcc gtattggata cgaactgctt ctgatacttc
     3121 ttttccataa agtttttcaa ctgtttgacg tacaactcca caaatttcgt cagcgatttg
     3181 tgcgtcagct gatttaccag taccgattgc ccagattggt tcataagcaa taaccatgtt
     3241 tgacacctgt tcagctgaaa gatcagctaa accagctgta atttgtcctt cgatccattc
     3301 agcagttttg cctgcttcat aggtttctaa agattcgccg cagcatagga ttggtgtcat
     3361 accatttgca aagatagctt tcgctttttt attgatgtct tcgtctgttt catggaaata
     3421 ttcacgacgt tctgaatgtc cgatgatcac ataatgaaca cctagatcag caagagctgc
     3481 tggtgaagtt tcaccagtga acgcacctga gttttcccag tagcagtttt gtgctgagat
     3541 ttttagttct gtaccttcag ctgatgtcat taattcttgt aagaataatg caggagatcc
     3601 gatgactgaa tcaactttgt catttgaagg gattttgttt tttactgctt cagcaaattc
     3661 ttttgcttct gatgcagttt tgttcatttt ccagttacct gcgataatag gtttacgcat
     3721 tgaggaacac actccttaat ttattgaaaa atggctaggg agtcatgaaa actcccttca
     3781 ccttttgttc gattactcgt cagaattgag caaacttggt cgtttattta tcgttgattg
     3841 ctgctagtcc tggtaattct ttaccttcaa gcaattccaa gcttgcgccg ccacctgttg
     3901 agatgtgtgt gaatttgtca gcaaatccta attgttctgc tgctgcggct gagtctccgc
     3961 caccgatgat tgttgttgcg ccttctaagt tagcgattgc ttcacaaaca ccgattgtac
     4021 ctttagcaaa gttactcatt tcaaatacac ccataggtcc gttccaaaca actgtttttg
     4081 caccttgtaa tgtatcagca aataatttta ctgatgctgg gccgatgtct aaagccatca
     4141 aaccatcagg aatgtctcct tcgattactt gtgtttcaac atcatttgaa aattctggtg
     4201 cacagacatt gtcgattggc aagacaagtt tgtcgcctgc tttttcgatt aattcttttg
     4261 ctagttccac tttatcagct tctactagag agttaccgat cttgattcct tttgcttcgt
     4321 agaatgtgta agtcattccg ccaccgatca agattttatc tgctttagga atcaagtttt
     4381 cgattacgcc gattttatca gaaacttttg cgccaccaag gattgcaatc attggtcgtt
     4441 ttggttcttc tactgcttca ccgatgaact tgatttcttt ttccatcaag aatccagcaa
     4501 ctgttgggat accagttgaa gcgataccta cgttagaagc atgtgcacgg tgagctgtac
     4561 cgaaagcatc gttaacaaat acatcgccta atgaagccca gtatttacct agttctgtat
     4621 catttttgct ttctttttta ccgtcaatat cttcaaatcg tgtattttcg aatacgacaa
     4681 cgtcgccgtc attcatgttg cggattgctt cttctaattg ttctccgcga gtttcaggta
     4741 cgaatgttac ttctttacct aataattcgc ctagacgttt tgcaactgga gctaaagatt
     4801 ttccttcttt atcttcttct gtttttacac gtccaaggtg agagaaaagg attgcttttc
     4861 cgccttgttc tagaacgtag ttgattgttg gcaatgctgc tttgatacga gtgtcgtcag
     4921 tgatcacgcc gtctttcaaa ggaacgttga agtcaacacg gacaagaact tttttgtctt
     4981 ttaaatcgat atctttcact gttttcttag ccattttatt tcctcctgaa taaacttatt
     5041 atcaaaaaaa gcggaagaag cgcgattgct tccccgcttt taaatgtaca tgtctataat
     5101 ttacacttgt acgtttgtct atcttataag ttagcgaagt attctaaagt acgtactaat
     5161 tgtgcagtgt atgacatttc gttgtcgtac caagcaacag ttttaactaa ttgtttgtcg
     5221 ccaactgtca ttactttagt ttgtgtagca tcaaataatg aaccgtaagt cataccaacg
     5281 atatctgaag aaacgatttc gtctgtgttg tatccgtaag attcgtttga agcttctttc
     5341 attgctgcat tgatttcgtc aacagttact tctttttcaa gaactgttac taattctgtt
     5401 aatgaacctg ttggtacagg aacacgttga gcagctccgt ctaatttacc gttcaattcg
     5461 ggaattacta aaccgatagc tttagcagca cctgttgagt taggaacgat gtttgcagca
     5521 gcagcgcgtg cacggcggaa gtcaccttta ggatgaggtc cgtctagagt catttggtca
     5581 cctgtgtaag cgtggatagt tgtcattaat ccttcaacaa caccaaattt gtcgttcaat
     5641 gttttagcca taggagctaa acagttagta gtacatgaag cacctgagat aactgtttct
     5701 ttaccagtta atgtttcgtg gtttgtgtta taaacgattg ttggaacatc gtttccgcca
     5761 ggagcagaga taacaacgcg tttagcacca gctgttaaat gtttttcagc tgctgattta
     5821 gaagtgaaga aaccagtaca ttctagaacg atatctacgc ctagttcgcc ccatggtaat
     5881 tcttctgggt tgcggttagc taatacttta acttcttttc cgtttacgtt gaatgaacct
     5941 tcatgaactt caactgttcc gttgaaacgt ccttgagttg tgtcgtattt taacaagtgt
     6001 gccaacattt tagcatctgt caagtcgttg attgctacta cttcgattcc atctacatct
     6061 tggatacgac ggaatgctag acgtccaata cgtccaaaac cattaatacc tactttaact
     6121 gtcatttaag atttcctcct tatgaaaatc aaaaaaatat ttattttaaa gggttacccc
     6181 ttttaaaatc tcatttgcag cagcttcatc agtgatgagc cacgtttgtt ttggtgcatt
     6241 tttcatataa gctcgaattg ctttagcttt tgattttccg cccgcgattg ccatgatgat
     6301 cgggactttt tccaaatctt gcaattctat gccgattcta gggaccttat aaacgacttc
     6361 acctttttca ttgaaaaagt agccaaaaga ttccgcaaca gcatttgctt gtttcagcat
     6421 gactaattct ttttcagtca tcttacgacg tgctgccatg tgtaatgcac gcccaatact
     6481 atgaataaca caattcgctt cactaatcaa gtttaagact tcttggatag atggctcatt
     6541 gagtaaagaa ttgtaagttg ccagacttaa ttgttccgga acataaagtg cgcggtggtt
     6601 accgtttgct ttagcagcca ttcttgcact gacagaattt gcctgtacag ttatcgcttc
     6661 accaatcccg cctcttgcag ggacaaacag gttatgccgt ttcttatttt ccagattaga
     6721 caattgttcg gctaccacag ccatagtagt tcctcccatc acagcgatga tattctcgcc
     6781 ttctggaagt agtcggtcta aggcttcgct cagcgcttcg ccgaatgcat cagccacttt
     6841 ggtctgctct tcgctgtttc ctgaaacaac gatacagcgt tggttaccaa aatattctgc
     6901 caactgttgt tcggtttgat gcgtacctaa aagctgatcc atgaagtttt caaggctttg
     6961 ataaacttct tcgcccttag ttgtcagcgt cataccgctt tttgaggtgt cgatcaaatt
     7021 cagatttttc aaaaggtctg tttctgttcg taagactcgt tctgtgatcc ccatactttc
     7081 cgataaactt cgacgcccaa tcggctgcat ccaatagata tttcgtaaaa ttttatagcg
     7141 ttcttgcaac acttcgataa tatctggtgc aacagattca atcattttta tttcatccag
     7201 catagcgatc tccttagttg gacactttac gtccaatgta gacttaaaac gacccatata
     7261 cgataaaaaa agaaattaac tattcgaatt tcacatcaat aaagggaaat ctcaacctca
     7321 cgactgtaag tctaacagtt ttgctctcgt ttttcaacta aaaagactag aaaaagtgaa
     7381 atactttttc tagtcttttg actgattagt tatcataacg ttttcttttt gatgaactcg
     7441 ggattcctaa taaatcacga tattttgcta ctgttcgtct tgagatcgct atctcatctt
     7501 ttttcagcaa ttcaaccaat ttctggtcag acaacggctt gtttttgtct tcttgatcga
     7561 ctaattcctg cagctttttc ttcgctgtat ctgctgaaag gtcttctgtc tgttctgtac
     7621 tattcgtagg aattcgtgta gtgaaaaatt tttttaattc aaatacaccg aagtctgttt
     7681 ccaaatattt cccgttgact gcacgactga ccgtggattc atgcacatct atctcagttg
     7741 caacttcctt taacgtaaga ggcttgattg gacgttcctt gtcgataaag aaagcttttt
     7801 gccgggaaac aatgacttga gcaacccgca aaattgtatc cccacgttgt aggatcgttt
     7861 tcttcaacca ttcgaattcc tgtttccgtt cttttaaata attttcagtt tctttatctg
     7921 cttgttgtga catttgtttg aagtaactct cttgaaagcg tatatttggc tgattttttc
     7981 ggtttgagat aacttgtaca tttttatcgt cgtccactaa tacccggaca tcaggaataa
     8041 tgtacaattc gctgctacgg tcgaaaatcc ggccagggct tggactcaac gtttgtaaat
     8101 agtcgaatat ctgctgaaca gcatgtagat caacagaaaa acgttgtgcg atttctttcc
     8161 attttcgctc caccagttcc tcaaaacatt cttccaacac gatataagct aattccggcg
     8221 cataatcatc tcgttctgtt tgaagcatga gacattcttg caaactacgt gcacctactc
     8281 cagctggttc taattgctga atcagagtca acgcatcgag catttggata ggcgtagctc
     8341 caatctcttt tccggcttct tctaaactaa tcgtcaaaaa tccgttcaaa tcgatgtatt
     8401 ctacaagata aagaacgatt ttcctcaaaa aagtatctcg gtaattcaaa tggacttgtt
     8461 cgatcaatga atcaaataaa gacccttttg tatcaggaat ctgatttagg taatttgtct
     8521 cttctccttc ataagaagat gaagaagctt ttatgtaatc tggctgccat tccggctcaa
     8581 caacttccac caatggattt tccattgctt gattctcaac gaatgccaac agctcctcac
     8641 tgttatattg taaaacttgg atggactgtt gcaactgttg cgtcatggct agtttctgtg
     8701 tctgctgctg tttttgtgaa aaattttgct caaatttcat ttttttgtcc aatccccttg
     8761 taatttttta tctgtttagt tacaattatc ccagtgcttg cgtccttagt gtagtggata
     8821 tcacgtaaga ttccggttct tgagacgggg gttcgattcc ctcaggacgc gttctactgc
     8881 tattttagca taatttacga atagttgctt ctttattttt aagtgaaaac tgtccgccaa
     8941 agaa
//
LOCUS       DAYYME010000113         8806 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000113 DAYYME010000000
VERSION     DAYYME010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.114"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(126..416)
                     /locus_tag="ACHBFW_002248"
     CDS             complement(126..416)
                     /locus_tag="ACHBFW_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318519.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(534..794)
                     /locus_tag="ACHBFW_002249"
     CDS             complement(534..794)
                     /locus_tag="ACHBFW_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318520.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(931..1344)
                     /locus_tag="ACHBFW_002250"
     CDS             complement(931..1344)
                     /locus_tag="ACHBFW_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFM9318521.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1800..2285)
                     /locus_tag="ACHBFW_002251"
     CDS             complement(1800..2285)
                     /locus_tag="ACHBFW_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318522.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2424..2639)
                     /locus_tag="ACHBFW_002252"
     CDS             complement(2424..2639)
                     /locus_tag="ACHBFW_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFM9318523.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2863..3663
                     /locus_tag="ACHBFW_002253"
     CDS             2863..3663
                     /locus_tag="ACHBFW_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9318524.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3682..5550
                     /locus_tag="ACHBFW_002254"
     CDS             3682..5550
                     /locus_tag="ACHBFW_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM9318525.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5543..6034
                     /locus_tag="ACHBFW_002255"
     CDS             5543..6034
                     /locus_tag="ACHBFW_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFM9318526.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6022..6576
                     /locus_tag="ACHBFW_002256"
     CDS             6022..6576
                     /locus_tag="ACHBFW_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFM9318527.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6594..7589
                     /locus_tag="ACHBFW_002257"
     CDS             6594..7589
                     /locus_tag="ACHBFW_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFM9318528.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
     gene            complement(7731..>8806)
                     /locus_tag="ACHBFW_002258"
     CDS             complement(7731..>8806)
                     /locus_tag="ACHBFW_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM9318529.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         3044 a         1424 c         1681 g         2657 t
ORIGIN      
        1 aataacataa taagaaagta aagtggattg atttgagatc tttttatact tcaaaaaaga
       61 taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta atacatttta
      121 atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag agagatcata
      181 agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc ccatatttct
      241 tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat ccagcaccat
      301 ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg aagtaaaaac
      361 aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc ccccacattc
      421 aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt atgaataaca
      481 gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga agtttatttt
      541 tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa gccgatttcg
      601 tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt atggataaaa
      661 tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa taagtaaaag
      721 aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa gacaatcaaa
      781 tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt aaaaacaaaa
      841 tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat atataaaaat
      901 cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt ttgtttcttt
      961 tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa aggttccagt
     1021 gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga ccgaatgtct
     1081 ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat gattggcgga
     1141 gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac agccaggcat
     1201 atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg gataacttga
     1261 aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag ggaacatgtc
     1321 gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt ttttatttat
     1381 ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt agctttgtga
     1441 atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg atcattaaaa
     1501 taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca aatatagaaa
     1561 aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac cgaaaatgaa
     1621 gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc ataagagaaa
     1681 tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta taactttctc
     1741 agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat atcatctaat
     1801 caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga cactcgataa
     1861 aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc tgtgaacaaa
     1921 taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg taattcggaa
     1981 cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag agaaggtaca
     2041 gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact tgattggcta
     2101 tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga agtactttta
     2161 gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga attctttgtt
     2221 ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta aatcccttta
     2281 tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt aaacaaagaa
     2341 aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat gaagataata
     2401 acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt aatcattggg
     2461 aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat cttcgcaaaa
     2521 atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa gcctaccaac
     2581 agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa agttttcatt
     2641 agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa cattgcttat
     2701 ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt tgctcttcca
     2761 ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt ttagtgagct
     2821 tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga ttggttggat
     2881 attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag aagtatcgtc
     2941 gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga atgccgtata
     3001 cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga catagaagaa
     3061 gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa taatgcagga
     3121 ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta tgaattatca
     3181 gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt gatgagccaa
     3241 gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct ttcttctgaa
     3301 tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc agcgatgaac
     3361 agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt ggtaggtgtt
     3421 gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga agctctttct
     3481 tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac aagtacgatc
     3541 cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta ttatctttca
     3601 gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa aacaagagga
     3661 taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt attgaaatta
     3721 ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa aaccagtaat
     3781 caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat tgcaaaaatc
     3841 atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt tgatgagatc
     3901 atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag aatcgcttat
     3961 ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt gtctgaagaa
     4021 gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga gatgatcaat
     4081 gattttgatg taaagattac aggcacacca aataaaggac taagaataat aggagatgag
     4141 ctagaattac gactactttt attttatttt gtttctccct acttttcaga gacgaaaaca
     4201 gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac gaatgctagt
     4261 aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag aatctctagt
     4321 aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata tatccaagaa
     4381 ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag ccaatatgaa
     4441 aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat gataaggaaa
     4501 gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg acgattagat
     4561 ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct ctctttattg
     4621 ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga gatagaaaaa
     4681 aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga agctatcttg
     4741 caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga gttagcattt
     4801 cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac gacaggaaaa
     4861 ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga tatacaaata
     4921 acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt cgctgttttt
     4981 acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt gactaatctt
     5041 tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg attagccggg
     5101 tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta tcaagaatat
     5161 ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga atttgaaaaa
     5221 aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt tgcttttcct
     5281 cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat aactgatcaa
     5341 ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc tgaagaaatg
     5401 acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga actcataaaa
     5461 atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt aaaactaatc
     5521 aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt tcgcagcttc
     5581 agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca atcaaacata
     5641 tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga aaatccgttc
     5701 aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag tcagaaaaat
     5761 gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg gtgaagatat
     5821 ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga cacaagcagc
     5881 tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa aaagtgaaca
     5941 accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa gtacgatcaa
     6001 agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg atttatgagg
     6061 ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt accagttgtc
     6121 ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga ccgagtaacg
     6181 aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt accgttctta
     6241 tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct tccagaatac
     6301 tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa tggtgtattc
     6361 ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg gatcgaaacc
     6421 ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact gctgatgaac
     6481 tttattggtg gttggataac tgattttact actggatttg tatgcagaca gcaaggaatc
     6541 gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt taaatgtatg
     6601 gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt attaggccga
     6661 caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca atgatcgtag
     6721 ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca tcaatacctg
     6781 atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt ggaatttatc
     6841 ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc ccattagcac
     6901 aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata cagccactaa
     6961 tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa aagtacaaat
     7021 atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg atagcaaaaa
     7081 ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga gaagcagtag
     7141 atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg attggtgtga
     7201 tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta gcaggaaatg
     7261 cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct cctttgcttg
     7321 gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag attgggaaag
     7381 gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact caaaatgcat
     7441 gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca gtagaagtag
     7501 gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt atcattgctt
     7561 gggtagcaag ttttggatta tatagctgag tttagtctaa tggtgataag tcaagatgaa
     7621 atttaagatt ttctttattg agtaatatgt tttgaagcgc acgaaactag acccgatcaa
     7681 aatcaaattt ttttcgggtt gattcaagga tttgcgtcaa taacggattg ttatttctct
     7741 atcacactcc ttggtggcgt atagagttgg tgtttacgta gtagcgtatc caccagacgc
     7801 gtaaattttc ttgcggttag gacgagtgca cgtttgtgtt gatgctttgg aacctcctta
     7861 tatttacttt gataaaaagc tttgtattca ggaagataat tttttacaga gttggcagct
     7921 tcaactaagt agtatctaaa atatcgattt ccttgttttg tcagaggtgt attttgcgac
     7981 tgatagtttc cagattggtt cactttccaa cttaatccag cgtattttgc caattttgtc
     8041 tgatcttcaa atctttcgat ttgaccaatt tcagcgatta aaccggcagc atagacttta
     8101 ccaacgcctg gaatactggt taagcattga tattctggaa tgacaacaat tacttgttcg
     8161 atggctttat ctaattcttt gatatttttt tcaagtgctc gtatttctcg tacgagaaca
     8221 cttaaaacaa cattaattga ttcttgtgca agagcaccta ggcggtaact catggtaatt
     8281 gcacgttgaa ttgctttagc gattttttct ggttgcttga aacggccttt tcctttttct
     8341 tgaagtaaat cacagaaagc ttctaaagga agctcagcaa gttcatccaa tgtgaaatct
     8401 tccgtcatga gtgaaatgag ggttgcacta aaaagactcg tcgattcgtc tcgcatttct
     8461 cgagcaagcg tattacattt gtacgttaga ttttcaagaa aatgttgctt tgtacgaatc
     8521 aattgcttaa taagttgata gcgagtacgt gttaaacgct gtaaggccat gtatttctct
     8581 tctttgatag gggaagttgt gaatcgttga atacgcaaga aatcagcgat tcttaatgca
     8641 tcgtttcgat cagttttatt ctcatcaaac atgcgactaa attgtttcac ccgaaaagga
     8701 ttttcaatcg ttacaagtgt gttgagcttc tttagtttct catcttcatg aaaaaacatg
     8761 gaaggatgga agctgtacat ggacgttgac tccatgccaa tcacaa
//
LOCUS       DAYYME010000114         8715 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000114 DAYYME010000000
VERSION     DAYYME010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.115"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..1095
                     /locus_tag="ACHBFW_002259"
     CDS             34..1095
                     /locus_tag="ACHBFW_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM9318530.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1098..1994
                     /gene="murQ"
                     /locus_tag="ACHBFW_002260"
     CDS             1098..1994
                     /gene="murQ"
                     /locus_tag="ACHBFW_002260"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM9318531.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2008..3990
                     /locus_tag="ACHBFW_002261"
     CDS             2008..3990
                     /locus_tag="ACHBFW_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM9318532.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4055..4501)
                     /gene="nrdI"
                     /locus_tag="ACHBFW_002262"
     CDS             complement(4055..4501)
                     /gene="nrdI"
                     /locus_tag="ACHBFW_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM9318533.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            4712..6136
                     /locus_tag="ACHBFW_002263"
     CDS             4712..6136
                     /locus_tag="ACHBFW_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM9318534.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(6317..7090)
                     /locus_tag="ACHBFW_002264"
     CDS             complement(6317..7090)
                     /locus_tag="ACHBFW_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM9318535.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7087..7992)
                     /locus_tag="ACHBFW_002265"
     CDS             complement(7087..7992)
                     /locus_tag="ACHBFW_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9318536.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(8146..8466)
                     /locus_tag="ACHBFW_002266"
     CDS             complement(8146..8466)
                     /locus_tag="ACHBFW_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM9318537.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         2730 a         1571 c         1867 g         2547 t
ORIGIN      
        1 aaatgaagac ggatacagaa aaagagggat aatatgtttg gcttttcaat atttatgaac
       61 gattctttgt cagaagagaa gaaacagtat atccgccaaa tggcatccaa tggctttgtc
      121 ggtattttca cttccatgca tataccagaa gatgatgttt cggcatatca aaagcgattg
      181 atcgaactag gaagcgaagc gaaaaagaat aaactagaat tgatggtcga tatttcagga
      241 gaggcattgg aacgagcggg tttttcatta gaagatctaa cgcctttgaa agcaattggt
      301 gtaacaggtt taagaatgga ttatcatatt tccaatcagc agattgccga ctggtcacat
      361 caattgaaaa tcagtttgaa tgctagcacg atcacgccta aagatatcga tgaattgaaa
      421 gaagcagaag ctgatttctc acagattgaa gcttggcata attattatcc acgtcccgaa
      481 acagggctgg acaaagaatg gtatcagaaa aaaaatcaat ggctcaaaat tcaaggtctc
      541 cttgtccaag gttttgtacc aggagataca gagttgagag gcccactata tcaaggattg
      601 ccaactttgg aagaacatcg agggatccat cctcttgcag gcgctctaga tctattagca
      661 agtaacacgg atattgttta catcggagat gcaggtctgt cagaaaacgt tcaggagcaa
      721 tttgcttctt tccaaaaaga acagacagtc ttgctccata cggaacctgt agatgaagaa
      781 ttttacgaat atatattagg gaagcatacg aatcggcaag acgatgcaag agatgtcata
      841 cgaagcgtag atgcacgttt tcgagagatt ccgcctatcc cagcaagaaa tacagctacg
      901 cgtatgaaag ggtctattac tttagataac gaaaagtatt tgcgctatat gggtgagatc
      961 caattaacca aatatgattt gccagcggat gaaaaagtga atgtagtagc taaagttatc
     1021 aaagaagagc taccattgat caaccaaatc aaagctggta tgaactatca atttataaga
     1081 aaagaaggaa gataaaaatg agcaaaatca atttagagaa cctaacaacg gaacgcagaa
     1141 accaagaaac ttttggatta gatgagatga gtatttctca agccgttcaa ctaatgaaca
     1201 aagaagatca gaaagtggcc gctgcggttg aaaaagaact gacaagtatc gaaccagtga
     1261 ttgctaacac gattgagtcc tttaaaaaag gtggacgtct gatttatatg ggagccggaa
     1321 caagtggaag actaggtgtg ttagatgctg ctgaatgcgt accgacattc ggtgtagaac
     1381 cggaaatggt cataggtcta attgctggtg gagaagaagc aatgactgta gctgtcgaag
     1441 gggccgaaga ttcttatgag ctagggcaag aagatctaat caagctgaat ttgacaaaaa
     1501 atgatatagt tattggtatc gcagctagcg gtcgtacacc atatgtgatc ggtggattag
     1561 attacgcaaa atcgatcggt gctgtcacag gaacgatttc ttgcaataaa caagccgaaa
     1621 tcagtaaaca cgcggatttc ccaatcgaag tcgatgcagg tccagaattt ttgacaggct
     1681 ctactcgatt gaagtcagga acagcacaaa aacttatctt gaatatgatt tctactatca
     1741 gtatgattgg tataggtaaa gtattcaata acttgatggt cgatgtcaaa ccaacaaatg
     1801 aaaaactaat cgaacgctca aaacgaatca tcatgcaagc gacagaagct gattacgaaa
     1861 cagctgaaaa gtactttgaa gaagcagatc aaaatgttaa gctggctatc gtcatgattt
     1921 tgacaaatag tgataaagag gaagctcaag caaaattagc tgaagcaaac ggatttgtaa
     1981 aagggacctt atgaggggga aaagaccatg gcaaaaaacg aactgacttt ggaagaactg
     2041 gcaaaacaaa tttataaagg ggcaggcggc atgggcaatg tagaaagcat tgtccactgc
     2101 atgactcgtg tgcgtatgaa gatcatcaat gatgatcttg taaatgtctc tgaattaaaa
     2161 gcgattccag gagtgcttgg tgtagtagaa gatgaacagc ttcaagtcat catcggacct
     2221 gggaaagtga acaaagtagc acagcaaatg gttgatttgg caggcgtgaa attaggagaa
     2281 aaactttctt ctgtaagtac agctacagca aattcatcag catctggaaa agaccgtgta
     2341 aacgaacggg cacaagaaat gaaagcggca caaaaatcga aacaaaaacc atctaaaatc
     2401 aaatcggtat taaaagacat ttctaatatc tttgtgccaa tgattcctgc atttgtcggt
     2461 actgggatcg tggcagggat tgccgcagtt ttagcaaact tagtcacagc cggaacactt
     2521 gatgcagcaa catggcaaca atatatcgat gtgatgaaca tcttgaaaaa cgggatgttt
     2581 agctacctag ttatctatac aggtatcaat gctgcacaag tttttggtgc aacaccaact
     2641 cttggtggag ttatcggggc cgttgtgatg ctgacaggga tgaatccgga agcaccactg
     2701 aaaaacttat ttactggtga agctcttgca gctggacaag gtgggatcct aggtgtcatc
     2761 ttcgctgttt ggctattgtc aatcgttgag aaaaaactgc ataaagtagt acctgatgcg
     2821 gtagatatca tcgttacacc aactctttca ttgattgcca tcggtctgat tgaaattttc
     2881 ttcatcatgc ctttagcagg tttcatttct gatggcatgg tcggtgggat caactgggta
     2941 cttggcgttg gtggagcatt tgccggcttc gttctaggca cattattctt gccgatggtt
     3001 atgttcggac tgcatcaaat tttgactcca atccatgtgc aaatgattga tgaaactggt
     3061 cgtacgctat tgcttccaat tcttgcgatg gctggtgcag gacaagtggg tgcagcattg
     3121 gcattatgga ttcgctgcaa aaaagataaa gaattaacag aaatgatcaa aggagcttta
     3181 ccagtcggga tcctaggaat cggcgaacca ttgatttacg gagtaacatt acctctagga
     3241 cgcccattta ttactgcttg tatcggtgga ggtatcggcg gggcagtgat cggaatgatc
     3301 ggaaatgtcg gggctatcgc tattggacct tcaggagccg ctttgatccc attgatctct
     3361 gacggtaaat ggttaggcta catccttgga ttattggcag cttatgctgg tggcttcgtt
     3421 gctacattct tcttcggtat tcctaaagac cgtttagaaa aagaagaatt agcgactgaa
     3481 acagccacag taaatgaagt gcctgaaaaa gtagtacctc aaacttcagc tgaaagtgtg
     3541 actatcctct catcagttgc tgacggaaca gttgtttctt tagataaagc aagtgatcca
     3601 gtttttgcac aaaagatgat gggtgaaggc tactttgtag aaccagaaaa cggacatatc
     3661 tattctccag ttactggggt agtcagctct gtcttcccaa cgaaacacgc tattgggatc
     3721 acaacgccaa atggtttaga aatcttgctt catatgggaa tcaacacagt tgacttaggc
     3781 ggtacgccat ttgatgtgaa agtaacagaa gggcagcaag tgacacctga tacgcttgta
     3841 gcagaagctg acttagaagc gatcaaagca gcaggcaaag aaacatccat gatcgtcttg
     3901 gtaaccaata tggatcgtgt aaaaaacttt gtcttggaaa aaacaggtaa agcaaaagca
     3961 aaagcacctg tgatggatgt caaagcttaa tatgatagaa acaatagaaa cgaaagaaga
     4021 aactgaaatc tatttttcag cttcttcttt tttattattt attgttttct gctaaaatct
     4081 gataaattcg tgtcacatca gatggtgttc cccgtaattc ataatctgcc aagaaaggaa
     4141 agccaaatgt ttgtgcatat tgtttggctg ttagacaata ctgattattg aagtttttat
     4201 ttccgctacc gactacacca agacaaagtt tatgattgtc ttcatgttcc agatattcac
     4261 gcatcgtttc ggtcaagatt tctgtatcac cgttatctaa accatttccg ccgtctaaat
     4321 aagtaggaac gaaagtaaaa aatggttctg tttctttcgt aaaatcacta ttttcatgga
     4381 tttcttttaa agtaacttca ggattgacag gatcttctgc atgcattttt tcagcatatt
     4441 ctgctagatg tttggcaaag gcacgcgtat ttcctgaaat cgatatatat aaaatgttca
     4501 tggatcatcc tccaattctt tccttttagt atagcatctt ttttaagttt tttggtggtg
     4561 taatttggaa agggactagg cgctttgctt gtataatgag aaaaaagccg gtagaattat
     4621 atttctatta aagtttttat taattaagta aaaataaaaa aggtttgtta tactttttga
     4681 taaaagcggt tttattttgg aggatgctta catggataca gagcaacaga aaaaaggaat
     4741 tggtttaatt ccacttgctg ccttagtaat tggttcagcg attggaggtg gcgtatttgg
     4801 tattatgacg gacatctccg gatcggctgg cggcccagct attttcagtt gggtactcgt
     4861 aggaacagca atgttgatgc tctccttgtc gatcaataat atcaataaaa aacgtccaga
     4921 tttaaaaggc gggatcttta gttacgcaga agaaggtttt ggccgtttcg ctggttttat
     4981 cagcggttgg ggttactggc tcacatgctg gttaggcaac gtagcttttg caacattgtt
     5041 gatgagcgca ttaggatatt tcttcccagt atttgaaggt gggcaaaatc tggcatcgat
     5101 cgtgatgagt acagtctttt tatggggata tgcatggcta gtgaatcggg gagtcgaaaa
     5161 tgctagtttc gtcaatgcta tcgtcacatt ctgtaaatta gtgccattat ttttatttat
     5221 tgtagtagct atcagcacgt tttcgatcca acaattcatt gataactttt ctagcgacat
     5281 tatattgaat gcgaatggac agccgatccc gttttccagt caagtattga gttgcatcat
     5341 ggtgatgctt tgggtatttg ttggtattga aggagcctct gtcgtaggtt cacgagcaaa
     5401 aaaacaatca gatgttggga aagcaacgat tctagggata ttcggcttga ttattattta
     5461 cgtcttggtt tccatgcttc catatgggac aatgacgatg aatcagctgc aaacgatcaa
     5521 ggcgcagcct gctatgggct atgtgtttga aatgatggtt ggtaaatggg gagcagtcgt
     5581 gatcaacggc ggtttgatca tttctttagt tggtgtttgg ctctcatgga cgatcttacc
     5641 gattgaaaca atgggaaaca tggctgaaga cggacttttg cctaaagcat ggggaaaact
     5701 caacaaaaat ggggcaccaa cttatgctat cgtattgacg acgttatgta cgaatgtctt
     5761 tttactttca ttattattca ctgacagtgc ttataacttt gcttacacac ttggtacagc
     5821 agctattttc ttcacgtggc ttttcttagg attgtaccaa atgaaacttt cttatcagcg
     5881 gaaagaatgg gtccaatttt tagtagggct tttagcaagt gcctttcaag tatgggctat
     5941 gctagtcgta gcctttaatg aagtaatgct tgttcttgtt cttttcttgc caggcattta
     6001 tttttacctc aaagcacgta aggagcaaaa cgagcctgta caacatatcc acttagataa
     6061 gattttactt ataggtgtga cgatggtcgg agtgattgca atggttttat ttgccactgg
     6121 gatgcttcac gtatagaagt ataaaagaag tataaagaga ggttgtgagc ctgtcgttta
     6181 ttcaaaaaca tactaggaaa ggagctgtga cacaaatgtt gtcacagctc ctttttcaga
     6241 ataaacggtg tttaagagac agccaatcgg caataaggct gaacgtctct ttcacagcct
     6301 cttttttgat gagtcatcat acattttttc gcttaaatac gagatatcct aatgctaaga
     6361 acaaaacgat gtaagctaaa ttaccgaata gcatttgatt agtagaaaga tgagtcaatt
     6421 gcttcatgat ctcttcgttt ccaatttggt tttgcagatt caacatactg atcggattcc
     6481 atttgatcca ctcccatttt tggatgagtg caataaggat accagatatg attgacgaag
     6541 caaaataaaa gacgattcct gctgagatgg atgcgccgga actattgata aagcaagcaa
     6601 tcatcaacac taaacttaaa atcagccata aaccgatata actacccaaa gtataagtga
     6661 acaagctttg gatcagtgtt tgaccagtag aaaccttctc cgtgaaggaa atcgaaggga
     6721 aaagaatcag tttcatcaac acagtgacaa taatagctaa aaggtataaa taaacagaat
     6781 aaataactaa tgtgatccat ttgcttacta atatttctcc tcgagaatac tttcgataaa
     6841 ggagattttt caatgtacca tattgtgctt ccatcgaaat gatcgtgctt gcagctgcaa
     6901 tcatgataaa gacaatccaa gatgtggcac taaaaagctg agcagtcatt tcaactggat
     6961 caatgatcgt cgtgtatttt ttggctagaa gtgctgttcc tatgagcaaa acaactaata
     7021 gaacagataa aacagcagtt gactttttct tgatcaattt aaataattct tgttttacaa
     7081 gcaatgtcat ttttcttctt ccccctttac tgaatccaga atttccaata gagaagattc
     7141 caaatcattc gtcagatgct ggacgtctgt cacatggata ttttgtgcag acaatagctg
     7201 gatcacggtt cctaataatt cttcgttttc ttttaaaagc ttgattttat caggatcttc
     7261 caaagtatat ccagcttctg tcaagatcgt tcgagcagct agatcatttg ttgtagagat
     7321 aacgatgaaa tcttgcgctg tttggttcaa ttcagccatt gtacattgtt tgatgatctt
     7381 tccttgattg atcaggatca gctcatcgac cattttttca agctcgctga gtaagtgact
     7441 ggagatcaag aaagaggtgc cctgagtagc caagtcggta atgatttttc gcacatctct
     7501 tgttgcttgt ggatccaaac cgttcatcgg ctcatctaaa ataacgatag acggatggtt
     7561 cagtaaggca agagcaattc ccaatttttg tttcattccc aacgagtagg atttcgcttt
     7621 tttgtgaatg tacttttcca tcttgagctg attcacgata gtgtcgatag cagatacatc
     7681 gttggtttca gagaataact tcagatgatc atagcctgtt aagaatggat agatacccgg
     7741 gtattcgatc aatgctccta cttgttccaa ccctttatga ctagagggag aaatctcatt
     7801 ttcaccaata cagattgttc catctgagta atggatcagt cctaaaatag ctttcatgat
     7861 cgttgttttt ccagcgccat taggacctac taatccaacg attttgccag gggataagct
     7921 aaaactgaca tcgctgaggg cttgtttgtg accaaagttc ttggataatt cttggaccga
     7981 aagacttttc aaaaatattc actccttttc tatagatagt acaagcatac acctgatgag
     8041 tggagaagtc gctaggtcat tggacttatt tcttattttt tcataaaaaa attgaatatt
     8101 aaataaaaag gcttattttt tctgatcagt acttattcca agtgtctagt ggccgctagt
     8161 aatcttgatg ccaatgatac tgatgagtag catccccaca aagaaccaag tggaaagatt
     8221 cagcttatca tgaaacaaga ccacaccaat tatgaccgaa ccaactgcgc caatccctgt
     8281 ccagatagga taagagagac tcatcggcaa acttttgatt gcctttgaca aaaagtaaaa
     8341 actagcaatc atgccaatca ctgtgtaaaa agaataattg attttagaaa aaccatcact
     8401 ccatttcatc atcgttgacc agaatacttc caaaattcct gctatgatta attccatcca
     8461 tgccatactt acattcctcc ttaactataa aaaaatacac aaaaagagga aaatcatccc
     8521 ttcaaagacc taatggaatg atcatcctct ttatcctacc cggtggcaga ttgtatatct
     8581 ttttctgtag tatagcacac ctatccagat atcggcaatc tcatactttt tagtaaagtg
     8641 taccaagcac tgacggatat atcgttagcg gaaaagaaga tgaatgcttt gcaatttcat
     8701 agaaagaagt tgtta
//
LOCUS       DAYYME010000115         8608 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000115 DAYYME010000000
VERSION     DAYYME010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.116"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(206..2587)
                     /locus_tag="ACHBFW_002267"
     CDS             complement(206..2587)
                     /locus_tag="ACHBFW_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM9318538.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2577..3224)
                     /gene="recU"
                     /locus_tag="ACHBFW_002268"
     CDS             complement(2577..3224)
                     /gene="recU"
                     /locus_tag="ACHBFW_002268"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFM9318539.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3288..3827
                     /locus_tag="ACHBFW_002269"
     CDS             3288..3827
                     /locus_tag="ACHBFW_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFM9318540.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3917..4336
                     /gene="gpsB"
                     /locus_tag="ACHBFW_002270"
     CDS             3917..4336
                     /gene="gpsB"
                     /locus_tag="ACHBFW_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFM9318541.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4358..4725
                     /gene="rnpB"
                     /locus_tag="ACHBFW_002271"
     ncRNA           4358..4725
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHBFW_002271"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4934..6100
                     /locus_tag="ACHBFW_002272"
     CDS             4934..6100
                     /locus_tag="ACHBFW_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFM9318542.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6146..7642
                     /locus_tag="ACHBFW_002273"
     CDS             6146..7642
                     /locus_tag="ACHBFW_002273"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFM9318543.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7723..8406
                     /locus_tag="ACHBFW_002274"
     CDS             7723..8406
                     /locus_tag="ACHBFW_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM9318544.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8415..>8608
                     /locus_tag="ACHBFW_002275"
     CDS             8415..>8608
                     /locus_tag="ACHBFW_002275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318545.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2703 a         1566 c         1830 g         2509 t
ORIGIN      
        1 aacaagtaaa tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta
       61 agctaaatgt tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac
      121 agcttggtac atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac
      181 gcttgtagac gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg
      241 gtgtatggtc atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag
      301 gagcagatga agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag
      361 acgctggctc gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc
      421 tttgactagt agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct
      481 ggacatagct ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat
      541 attgatcttt gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt
      601 ctgtatttgt cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta
      661 cgtgtgacga ttctgaagtg acaggcgtca tttttttatt gtaacctgtc cacacggaaa
      721 tcgaataatt tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac
      781 caagttttgc atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata
      841 acccagcgat ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg
      901 tgatcatata agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt
      961 ctgttccgtc ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat
     1021 tggcgaaagc cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat
     1081 cttgttcttc cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc
     1141 caagattttt taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg
     1201 ctggaacatt acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat
     1261 tatcccagtt tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag
     1321 tttttccagt agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca
     1381 tcgtagaacc aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt
     1441 ctgcgatgtg acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg
     1501 tggaggcaac ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat
     1561 catatagttt cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt
     1621 agacattctt atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga
     1681 cgatcttagt attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat
     1741 taacagcttg atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa
     1801 cagtatctcg tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg
     1861 cttgaggcat cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac
     1921 caaagtacat ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat
     1981 ttacgtagta ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc
     2041 acgcttcttg ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa
     2101 gtttgatcaa ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag
     2161 agagggcaga accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat
     2221 cttctaccga cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt
     2281 cacgcttttc tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag
     2341 atacggtagc gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga
     2401 aaagtcctac acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct
     2461 tgataaataa tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct
     2521 tcttcacact ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat
     2581 ttgccattct tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc
     2641 aaataaggaa tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa
     2701 gataaaggta gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga
     2761 acactattca aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc
     2821 ccattttgtt cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag
     2881 ggaaaagatg ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag
     2941 acgccattat agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca
     3001 cttctcttag gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc
     3061 gccaacccgc gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc
     3121 ccccgattgc cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag
     3181 gcattcttat aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct
     3241 aggccacatt atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa
     3301 aagtaatcta tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc
     3361 ctaaaatcca agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag
     3421 ggctggaatg ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag
     3481 tagcggaatt gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg
     3541 attttggtaa taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag
     3601 cagattatgt cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc
     3661 acactcgttt tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc
     3721 ctggaaaaac gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg
     3781 aaattcggct gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt
     3841 tgatttatca acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa
     3901 aactagaggt gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga
     3961 attcaaaaca aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat
     4021 catcaaagat tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg
     4081 attgagtgca aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca
     4141 acaaacagag gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc
     4201 aaatcttgaa cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc
     4261 agcacaacct aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa
     4321 aacacgtcaa ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga
     4381 cttgatcagg ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc
     4441 ttagcgaaac cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct
     4501 tcggctatgc ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat
     4561 ccggtggaac gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt
     4621 gtctggaaga agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg
     4681 ttttttctcc ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag
     4741 ggaattaggt cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta
     4801 tttttcaata cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta
     4861 ttcggaattt gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat
     4921 aaggagattg tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg
     4981 gctagaagca ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa
     5041 cggacgtgca cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg
     5101 tacagcagat cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt
     5161 gtttgaaaac gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc
     5221 tgtagctgga aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac
     5281 aaaaaaagca atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact
     5341 gacggaaact ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt
     5401 aactctggat acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg
     5461 agcaccattg aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga
     5521 ccgtccattt tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat
     5581 aggccataat atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt
     5641 cagtcaagaa atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat
     5701 tgaattagat attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa
     5761 tgcagaagaa atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt
     5821 caaaacggat aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg
     5881 agaagaagaa actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa
     5941 aacatggagt aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa
     6001 agcaaccaaa aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg
     6061 gggaactcgt ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc
     6121 aattggatgt ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga
     6181 aatcaatctt ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc
     6241 tgaaaaagct agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc
     6301 aaaaaaaatc ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga
     6361 attgtcggaa gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca
     6421 tcgcgtaccg gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca
     6481 atggcaaaaa gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa
     6541 tattgaatta acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga
     6601 tgttttgctg aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga
     6661 tcagcttaaa gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc
     6721 tgaaacagac tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa
     6781 agtaatcgaa caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc
     6841 atttatgact ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga
     6901 tttcaaaatg gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga
     6961 catcgatgat aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga
     7021 atcacaatcg ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca
     7081 atatcctttc tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt
     7141 ttatcgttca ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac
     7201 ttaccctctc catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt
     7261 agatgttgct gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg
     7321 accaacaaat gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg
     7381 ttattttccc tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca
     7441 gcaagaaata gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg
     7501 gatgtcagag catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga
     7561 tgcaacacat gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt
     7621 taatgtatat caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct
     7681 atagatgttt tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg
     7741 atcgctgatt atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat
     7801 accaaaagag ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc
     7861 tttgtgacgc ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat
     7921 gagaacgagg caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa
     7981 cagatctcga tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa
     8041 gcggccgaac tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta
     8101 ggagcaatcg gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt
     8161 gatcctgaac tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca
     8221 gtgatcaatc atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac
     8281 ggatatgaag aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt
     8341 ctggaatggt ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa
     8401 aaatgatgga ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta
     8461 aaagttactt ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt
     8521 cacaaggaac aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt
     8581 cggaattggc aggagaacac cgtttatt
//
LOCUS       DAYYME010000116         8150 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000116 DAYYME010000000
VERSION     DAYYME010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.117"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            379..567
                     /locus_tag="ACHBFW_002276"
     CDS             379..567
                     /locus_tag="ACHBFW_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFM9318546.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            645..1658
                     /locus_tag="ACHBFW_002277"
     CDS             645..1658
                     /locus_tag="ACHBFW_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9318547.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1725..2759)
                     /locus_tag="ACHBFW_002278"
     CDS             complement(1725..2759)
                     /locus_tag="ACHBFW_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9318548.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2874..5099
                     /locus_tag="ACHBFW_002279"
     CDS             2874..5099
                     /locus_tag="ACHBFW_002279"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM9318549.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5117..7000
                     /locus_tag="ACHBFW_002280"
     CDS             5117..7000
                     /locus_tag="ACHBFW_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFM9318550.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6981..7955
                     /locus_tag="ACHBFW_002281"
     CDS             6981..7955
                     /locus_tag="ACHBFW_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFM9318551.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2644 a         1480 c         1712 g         2314 t
ORIGIN      
        1 agtctacatt attttggcga tttgccaata aggactgttg agttgtgcaa aatcaaatct
       61 gggtcagaat gaacgtcatt ctgtctcaga tttttcgtta tttcaatttg attaactgat
      121 tgatcaaaaa gatttttatt ttcatattct caaatgattc aacactaaat atacatgttt
      181 gacgcggaaa ttattattcg attttggcgg catcaggggg gatgacgacg tagcagaccc
      241 attattttac tcaagaaaat gtccgtgcct attatttaga atacgatagt gaacgtgctg
      301 ggagttttgt tcttttagag aaagtctctg aagataaatt agttgtctta gattttgact
      361 gtaacgaaaa atggcgaaat ggaagagcac cagcaagtga ttgaccggat ctacgaagcg
      421 acacgatatc tgcctttgga tcgtttgtgc ttaagtcctc aatgtggatt tgcttcaaca
      481 gaagaagaaa atgttttgtt tgaagaacaa tggaaaaaat ttcttttgtc aaaagcattg
      541 cagaagaagt ttggggaaaa tcattaaatc atctaggaga gcttgatagg aaataacact
      601 atggtataat cagaatgctt aatactaact actgagggag ccttatgaaa aagaaaattc
      661 ttaaatttat cggatggttc ttcggaatca ttcttgggat cgcgtttgca atctttcttg
      721 ccttccagct ttcccccaga cctggagcat tcttgatcaa ccatatgttt tcaaatactg
      781 ttcaaatcac tgataaaaaa acgttcgatc aagcagaaaa aaacgtggcg aaaaaaacag
      841 atttgacgta tgagtccaag tataaagaga atacttatga catctactat ccaaaaaaca
      901 gcaaggggcc tgttcctgtt ctattttggg tacatggtgg aggattcgtc ggcggagata
      961 aatcgggtgt caaagaattt gcgacaaaat tagtttcaga cgccaacatc gcagtggttg
     1021 cgatgaatta tgaattagcg cctgattcag aataccctaa tcaagttctt caagtgaatg
     1081 aactgatcaa agatttatta gctgaagaaa aagacgacaa attgctagac atggaacaat
     1141 tattttttgg tggagatagt gcaggttcac aaatcgcatt gcaatatgca gctatacaaa
     1201 cgaatgatgc gtatgctaaa caaatgaaaa tcaaacagct acttccaaaa gatagtctga
     1261 aaggaaccat ttcttattgc ggacctgttg atttgaagct aacagcacag cagcattcgg
     1321 atgatcgatt tatgaagttt ttcgtcaaaa ccgttgcgtg gtcgttgatt ggaacaaaag
     1381 attggaaaac gagtgagcaa ttgaaagaag ctagtgtggc tgaccatgtt tctaaggaat
     1441 ttccgccaac gtatataact gacggaaatg catactcatt ccaggagcaa ggtcaagcat
     1501 tagaaaatcg gttgacagaa ttaaaggtac ccgtgcaatc cttgttttat acagatacaa
     1561 aaaaagagat tacccatgaa taccaatttg attacacgct aaaagaatcg caaaattgtt
     1621 accagcagac attggatttt gtgaataagt ataagtaaga ggttgtgaaa aagccgtttc
     1681 gttttgagta tgaagaaacg aagcggcttt tcacaacctc tctttcaata cctttttgtt
     1741 cttgggataa aactttccct aacaatcaat cgtgcaggat aagtcaaaat gattggttcg
     1801 tcccgtatac cgtcaattcg ctccaatgcc attcgaacag ctgcttgccc aattgatttc
     1861 gcattgattt gaacactggt caagccaggg gagaggtttg cgattgtctc gatattatca
     1921 aagcttgcta caacaatctc ttctccaatt tggatatttt tttgtttcaa tgcccgatag
     1981 acaccaattg ccattggatc gctcccgaca agaatggcag taggaagagg ggattcattt
     2041 tctaatagat tagtcgcaag tacctcacct tgtttttctg tccagccacc tagatgataa
     2101 gacatatatt tttctaagtg atgatcgatc ataaaatgct gataggcacg catcctcttt
     2161 tcatttatag aaatctgctt cccaccgaat tcatccatat cgactttata tcccccgata
     2221 taggctattc ttttatgacc attttctaag aacaattcca gtacggtttt agtcatccgt
     2281 tcaagatctg catacaccat atcgacctct tgctggatgt cagggttatc cacgacgact
     2341 atttgcatgt tttgttttaa caaggcggta atcgattctt tcgtaacagt cccaacaacg
     2401 atcaccgcac ctaactgatc tagatcttcc cattgtttag gattatcaga tagattatag
     2461 attcgattca tccctaaatg cagacgtcga gcttcttctt ccatccctac tcgcaaatgt
     2521 ttgtaataga tatcctgttc ttcgctcgct ttgccgatcg tagagatcac accaatcgaa
     2581 ggcattctct ttttgatata tttacgagta ctgatgtcat aatccaacgc agcagctgcc
     2641 tctactatcc gttgccgagt agtctgagcc acatggatcg tctgatcgtt attcaacaca
     2701 cgggaaactg tagcaggaga gacatttgcc tttcgtgcga catctctaat tccaaccatt
     2761 tttacacact cctcaaaaaa ataaaaaatt tttattgaca aaattgttta gtaaacagta
     2821 tacaggaatt ataaaaaaaa gtaaacggtt tccgttttgt tgggagtgaa atgatggata
     2881 ttacgcatag aaaaacagat aaaaatttca tcttatttga tgaaaagagc cgagtcttcc
     2941 atttacgaaa tacatttctt tcttatctga taaagataga agaaagcaat gtattggcgc
     3001 atatttattt tggcaaacca gtcaagcagt acaaagacaa taaaaattat ccccgaagag
     3061 atcgtggatt ttcaggaaat gttcccttaa atcctgaccg gtctctgtcg aaagatacac
     3121 taccgcaaga atactcttct catggcagca tggactttcg aacgcccgca tcgatcattc
     3181 agcgcaagaa tggatcagac ttactggatc tgagatatga cagccattat attacagatg
     3241 ggaagccaga gatcgaaggc ttgcctcaaa cttatgtgat ggacaagtca gaggcacaaa
     3301 cgttggtgat ttcacttaag gatagagaaa cagcgattta tttcgatttg ttctatacca
     3361 tatttactga tagagcagtg atcacacgat ctgtaaagat aagaaatgaa accggagaaa
     3421 caatcaaact agaaaaagcg gctagtttcc agctagactt tgcccatact aggcgatttg
     3481 atgaagtcat tgctttgcca ggagcacatg tcaatgaaag acagatttca cggcaatctg
     3541 ttttatctgg aacaaaagta tttgaaagcc gtcgaggaac gagcagtcac catatgaata
     3601 actttattgc tttagttcat catcatacga cagaaaatac tggagaagcg atcggacttc
     3661 aatttgttta ttccggcaac cattctttcg aacttgaaaa agatcagatc aatcaattga
     3721 gagtcgtcgg tgggatcaac agccatcgtt tttcgtggga attgaatgca ggccagtctt
     3781 ttcaaacacc tgaaatgatc ctttcttact caagtcaggg attgaacaaa atgagtcaga
     3841 tccaccatga gttgctacgt gaaagaatcg caagaggtcg tcatcaattt gctgaacggc
     3901 cgattctggt caataactgg gaagccactt actttgactt caatagtgaa aaaatcaagg
     3961 cgatcattga tgaagcaaag gagctaggca tcgaaatgtt cgtattagat gatggctggt
     4021 ttggaaaaag agatgcggac aattcttcat taggagactg gttcgagtat gaaggcaagt
     4081 tgacaaatgg attacgtgaa atagcagatt atgctcatag caaaggattg aaatttggtt
     4141 tatggtttga acctgaaatg atctcggttg attctgaact gtatcgaaca catcctgatt
     4201 ttcttatgca ggagccaggt agaatgcctt ctgcttccag atcacaacat gtactggatt
     4261 ttacaaggtt agatgttcgg cagacgattg agaaacaaat gagaaagatt ttagatacga
     4321 ttcctcttga ctacatcaaa tgggatatga accgttcgct ttcagatgtc tattcgatta
     4381 cccttgaccc acagagacaa ggagaagtag cgcatcgtta tatgctcggg ctttatgaac
     4441 tacttgaaca tttgacaact gattatccgg aaatcttatg ggaaggctgt tctggtggag
     4501 gtggacggtt tgatgccggg tttatttatt atatgccgca gtcatggaca agtgataaca
     4561 cggatgcagt ggagcgtatg aaaatccaat atggaacaag ccttgcttat ccgatttcgt
     4621 ccatcacagc tcatgtatca gctgtaccaa atcatcagac cgacagatct acttcgttga
     4681 aaacaagagg agaaacagct atgagcgctg tttttggtta tgaactggat ttgacaaagc
     4741 tgtctcctga agaaaaaaag caagtaaaag accaaattat atcctatcaa acgattcgtc
     4801 cggtgatcca gtatggccat tattatcgtt tggcgagtcc ttttgaagaa aatattgccg
     4861 catggatgtt tgtgtctcct aaacaagatg aagctattgt gtttttaggg aggatactag
     4921 cttcagctca accagcgttc catgaagtat atctgatggg gttagatgat gaggcacttt
     4981 atcaggaaca gacctcgaag cggatatttt cgggggccga attgatgaca gttggacttt
     5041 acttccccga ttttcaaggt gatttccaaa cagaactgct tcatttcaaa aagttatgag
     5101 agagaaggaa aaaagtatga aagcaaatat aaaatggtta gatgatccac aagtttttag
     5161 agtgaaccaa tcaaatgcac atagcgatca tgtttgttat gcaacaaggg aagaagtaga
     5221 aaccacatcc tcccttaaac agtctttaaa tggatcatgg cgtttttgtt attcgccaaa
     5281 tccttcggta cgaccaaaaa cattttataa aaaagatttt gatgcctgtt cttttgatga
     5341 aatcactgtt cctagtcata ttgaattagc aaattatgac aagatccatt acatcaatac
     5401 gatgtatccg tgggagggac atgtatttcg ccgtccccct ttcacgacaa gccctgagaa
     5461 agaggcggaa gggatctttt cagctgctaa agacaatccg gttggttcgt atttgacgac
     5521 ttttcgatta gaagaaggtc taaagggaaa acaaatctcg attcattttg ctggtgtaga
     5581 acaagcaatg tttatttggt tgaatggtca ttttatcggg tatgccgaag attcgttcac
     5641 tccgtctgaa tttgatttaa caccttattt ggaagaagaa aatattctag ctgtggaggt
     5701 ttacaaaaga agtacagctg cattcttgga agatcaggac tttttccgat tttttgggat
     5761 cttcagagat gtcttcttag ttgctcgagg acaaaaccat ttagaagatc tttggatcaa
     5821 acctcgctta gatgacaaat gtcaaaatgg caaacttgaa gtagatttgt cttttgagca
     5881 agtagccaaa aacagtcagc tgaatgtagc gatactagat cctgatggga aggaaatcta
     5941 tcaacagcaa tatccgttaa aagagcaaat gacgattcgc atcgatggat ttgaaaatgt
     6001 ggcactgtgg gggcattttc agcccaattt atataagctt gaattggaga cgatagacga
     6061 agaaaaaaga gtaacggagt atattgacta ttccttcggt tttcgtaaaa tcgaaatcaa
     6121 agataaaatc atctacttca atggaaaacg gatgattatc aacggggtaa atcgacatga
     6181 gtggcatccg aaaaaaggac gcgccatcac tatagaagat atggaaaaag atttggctat
     6241 cataaaaaat gcaaagatca acggcgtacg gacatctcat taccccaacc agatcacttg
     6301 gtattattta tgcgaccaag caggaatcca tgtgatggcg gaaacgaacc tcgaatccca
     6361 tggctcatgg caaaaaatgg gcgcatttga gccttcttac aacgtaccag gtgcagtacc
     6421 tcagtggcag gcggctgttc ttgatcgagc aaaaacgaat ttcgaaacat taaaaaacca
     6481 tccaagtatt cttttttggt ctcttggaaa tgaatcttat gcggaagaaa acattcgaca
     6541 aatgaacgat tacttcaaaa aagtagaccc cgatcgactt gtccattatg aagggaattt
     6601 tccaaatcca gcattcgaag attcgatttc agatgtaaaa agctgcatgt atgcgccacc
     6661 agaagagatc attgactacc ttgaaaataa agcggaaaaa ccgttcatct tatgtgaata
     6721 tatgcatgac atggggaatt cgttaggtgg aatggattct tacatgacgt tgcttgatcg
     6781 ttatgaaatg taccaaggag gctatatatg ggattttaaa gatcaggcga tccaagtgac
     6841 tgatccaatc acaggcagag aagtgatgcg ctatggcggt gactttgacg ataaaccaag
     6901 tgactatgaa ttctcaggga atgggatcgt ttttgcagat ggacaagaaa aacccgccat
     6961 gcaggaggta agatattatt atgaaaaata cagtaaataa aagtcatatg gatacggaaa
     7021 aagttgcaat cgtcttcggc gactgtacat taggtgtcaa atcggggaat acgcattata
     7081 ttttttctta tacaagaggc ggactggaat cgctcaataa aaacggaaaa gaatggctat
     7141 accgggaaac acttccagtt ttttggcgag caacgaccga taatgacaga ggtagcggat
     7201 ttggatttaa atccggtatg tggttaggag ccggattgta tccaaaagtc aaagaaatag
     7261 aagtactggt ggacaataaa aagatcgatt tgccgattgc tccagtaaat aatcaatatt
     7321 caagtaatga atacgctgaa acggttgaaa tcatttttac actggaaatc aacacagtac
     7381 caaaaaccca agtgacaatt tcctaccaaa tcgctggaga gggtaaaatg cttgtcaata
     7441 tgcattatta tggacaaaaa ggattaccgg aattacccgc attgggttta aggttcatta
     7501 tgccaacgaa agcaaaattt tttgagtatg aaggcctatc aggggaaaca tatccggatc
     7561 ggatggctgg tggtgtcgaa ggtatttata aagtggcagg actccccgtt tccccatacc
     7621 ttgttcccca agaatgcggc atgcatatgg aaacgaaatg ggtagaacta acaagaaatc
     7681 aaacgttgga taatacggat aaagatcaag aagatttttc gttgcgattt gaacagcagg
     7741 atacgccatt tgctttttcc gctttaccat tcaaagcaga agaactggaa caggcaacac
     7801 atatagaaga actaccgcca gaaagacgaa cggttcttac gatttttgca aaagtaagag
     7861 gagtcggggg tatcaatagc tggggagcgg atgttgagtc accttaccga atcagcagcg
     7921 aagaggacca tgaaatcggc tttattattc agtagcaatc tttagattat tatttgaaaa
     7981 acggagttta caagacaaag ctgctttatt ctatatgtca tgaacaagag agtaaaaaag
     8041 ttctcagata cttttgttca tgactatttt cttcaataaa accaattgtt atttttctga
     8101 aaaacaaagt taactatcag aaaattaaaa aaagtttcat aaaaatgatt
//
LOCUS       DAYYME010000117         8146 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000117 DAYYME010000000
VERSION     DAYYME010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.118"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..541
                     /locus_tag="ACHBFW_002282"
     CDS             <1..541
                     /locus_tag="ACHBFW_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318552.1"
                     /translation="LNKLSKTTIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIF
                     YLFLMGYGLKEWNWKEKVRYKLPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSK
                     PYSLFAMYYTFSSFLFLEMLSDKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMK
                     REFPNSGKAWLINIGQFFI"
     gene            737..2572
                     /locus_tag="ACHBFW_002283"
     CDS             737..2572
                     /locus_tag="ACHBFW_002283"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM9318553.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(2632..3348)
                     /locus_tag="ACHBFW_002284"
     CDS             complement(2632..3348)
                     /locus_tag="ACHBFW_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM9318554.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(3575..4117)
                     /locus_tag="ACHBFW_002285"
     CDS             complement(3575..4117)
                     /locus_tag="ACHBFW_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFM9318555.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(4122..4349)
                     /locus_tag="ACHBFW_002286"
     CDS             complement(4122..4349)
                     /locus_tag="ACHBFW_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFM9318556.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(4428..6308)
                     /locus_tag="ACHBFW_002287"
     CDS             complement(4428..6308)
                     /locus_tag="ACHBFW_002287"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9318557.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(6497..7111)
                     /locus_tag="ACHBFW_002288"
     CDS             complement(6497..7111)
                     /locus_tag="ACHBFW_002288"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9318558.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            7253..7813
                     /locus_tag="ACHBFW_002289"
     CDS             7253..7813
                     /locus_tag="ACHBFW_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9318559.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         2483 a         1593 c         1330 g         2740 t
ORIGIN      
        1 tttaaataaa ttatcgaaaa caacaattaa atatctacta ttatttttaa caattggatt
       61 agttggctgc tctactctat ttaataaaga cccgtttgca ttacagaatg gacagagtat
      121 tttatggatt ttttatttat ttttaatggg ttatggtttg aaagaatgga actggaaaga
      181 aaaggtccgc tataagcttc ctcaagtatt tatcagcttg attgttttat tcggtttgat
      241 tattttgatg acacaaattt cactcatcat cagaggaaat acagatacag cgctgaggtt
      301 ttctaaacca tattctctct tcgcaatgta ttatactttc tcttcgtttt tatttttaga
      361 aatgctatct gataaattag gtataaaagg aagaggaaat tttcttgctg tatttttgat
      421 tgtcactcaa gttatatgta atttcccttt agtttcttat cgcgtatcta catttatgaa
      481 acgtgaattt ccaaactcag ggaaggcatg gctgataaat atcgggcagt tttttatttg
      541 atatttatta gcagtgtccc tcctagttat ctttttattt tatatacaaa aaatccgttt
      601 cattcatcgt ttcatcgaat acttcgcttt tgattcgatt gaacaattaa ctcagcgttg
      661 gaaaaaaaga attcattggc tttttgttca taaaagttat tttttagttg cagcattttt
      721 ttactgcttt accttcgtgc agattttcgt tttagaacca aaagaaggtt ggaaagaaac
      781 tatccaggtt gccttaaaaa tatttactca gcgacaagca cccatgatcc tgacaatcat
      841 cattattatg ggatttttct tcttactatt gttgatttcc aatcgttttt ggtacgcctt
      901 gactgctacc ttgatcatta acttactgct cactatttcg acagtaatca aaatggaaat
      961 gcgggaagag cctgtctttc cttcagattt aaaaatgctg accggattgt cagaactatt
     1021 atcgatggtt agcccagtct tattaatcgt gggcggattg atccttctcc tattattgat
     1081 tactagtatt attgtacaac gtagactaca aaaacagtat tccttgaaga ttcattggaa
     1141 acgtcgattt atcagtatcg ctgtattatt aggatgtttt tcgggtgtct ttttcattaa
     1201 tcacaaaaat tcgccttctt tcttgctgtt taatttattt aaagtaaaca agactttctt
     1261 taaccagcaa ggcgccgtaa aagataacgg accagtcatt caattcctaa acaatatcga
     1321 cattaaaatc atggaagaac ctgctggcta ttcgaaagaa aaaattaatg gaataatgaa
     1381 aaagtacaac acaaaggcag atgaaatcaa ccaaacaaga agtgactggt taaacaatca
     1441 gacgatcgtt ctcaatttaa gtgaaagctt cagtgatcct gcacgtgtcc ctaatctgac
     1501 agtacctaca gaccctattc cttacatcaa aagcattata acagaaggaa caggaggatt
     1561 gatgttatcc gtcggttatg gtggcggaac agctaacatg gaatgggaag gactgacagg
     1621 cttgagtatc agtaacctgt ctgcttcgtt agttacacct tatacccagc tagtggagcg
     1681 tcagaaaata agtccaaata ttaccgatct attcgatgaa aaaatagcca tccatccttt
     1741 tacagctgct ctttatcgca gaaaacaagt gtttgataaa tttggttttg acaggttcta
     1801 ttatttagac agtccggata aattaactta tacggataaa atagaacaaa gcccacgcat
     1861 atcggatgat tcagcataca aagaaacctt gaaagcttta caaagcaata cggaaacgtc
     1921 acaattcatc caattatcca ctatgcaaaa ccatatgcct tatgatgatt actatagtga
     1981 attgaactac actgctgaag gaactgctgt cattcctaat agaagaaaag aattacaaac
     2041 ttatatgcaa ggccttcact ataccgatac agcagtaaaa aaattcatcc aagaaatcga
     2101 caaaatcgat aaaccgatta cctttgtttt ttatggtgac catttacccg caatctatag
     2161 tgggaatgat tcgaaaaaat atggattaga acagcaccag acagattatt tcatttataa
     2221 caacaagtat agtcgtgaac aagcagctac gttaaataaa gaagttgttg ctcctaataa
     2281 ctttggtgct atggctcttg agcatgcgaa tatcaaagta actccttact atgcgttgtt
     2341 gacagaagta tctaatcatc tacctgcatt aacaattgat cctacagaaa gcttgtctaa
     2401 tcgttttaat ggaaaacagg tctttgttac agaggatagt caaattatcc aagaaaaaaa
     2461 tttaactgag aaacaaaaag aaattctaga agattatcgt ctgatacaat atgatcttgt
     2521 tgcaggtaac cagtacagtg caacatgggc gacacaaaaa ataaaagact aacaaataaa
     2581 ctgagcaagg ggttgtgata aaagacatgt cacaacccct tgtttatctc attaatttgc
     2641 ttgtccataa acgatttgat taaagccatt tttcaattca ttattcgctg tctcgttgaa
     2701 gtaaagataa ttatcgggat tcccctgatt ttgttgtgtc accaatactt ttggctgatt
     2761 ttgctgaaac ccgaatagat agacgtgctt tttcagatat ggttctgtct ctgcatcgga
     2821 atagacggct gtgatttgat agtctgcttg acctgtccca ctttctgacc attctgcaga
     2881 aacaggcact ccaccaatag ccatcttcca ctccccgttc aaaatagctt gagggacttt
     2941 aagaccatat aagtccacac tcatttgatt cgtataactt ttgtattgtt gtcccattgt
     3001 ttggccccat ttggtcataa atgcctctaa ttgtgcggat ttgtctgcat tccacagtgt
     3061 ttgttcaact tgttcgctgc tttctgaaga ggaagatgaa gcctctacag aagaacttga
     3121 agtagatgtt tgagttgttt cagtcgtttt ggaagactct gacgaagtag aggtatctgc
     3181 tgtttctgta cttgctgcag ttgacttgct ctctgtgcta gttgttgtag tagttgcaac
     3241 tgttgctttc gctgtttctt ttgttgattc ttttttcgat tcattcccgt tggtacaacc
     3301 agctaacaaa aaagctagac caattgttac taaaatcctt tttttcaatt aaaaaactcc
     3361 tttcgattac tcccacttct ttattctatc aaataagcaa tgtgatttta ttgatttttc
     3421 gtattttctc atcaatcttt caaatcttct aaaaactgag aagcacccgc tgatgttgtc
     3481 tgtaatacat cagcgggtgc ttcttttatt ttaatcttcg tgattttcca atttacctca
     3541 cgctttatat ttgtatttga agaaacatag acttttagtc atcatcgtct tcctcatcca
     3601 aatcttgtcg gacagttttt ccttggagtc tttgtacttg gtagatgatt tctttattga
     3661 aacttaaaag ttcaacaagc aattgttgca agccatattt ttcttctttt tcagctaatt
     3721 tgattgcccg tgtgataaag acattttgga aatcaaaatc ttttgcaaag ttttccaaca
     3781 ttgttgaggc tggtaaatat ttgttttgac catgttcgta aatttttcca taacgcatga
     3841 cttcttctaa agtagacgga acgatttcat tttcaatcac taactcatga gagattttat
     3901 tgaaccatct cgcttcttga tcagcatatt ctgaaaaaag ggtacttaat tcttgcgaag
     3961 atgtacccaa aacatacaat tgtgcttgga tcaatttcat ttcttctaat ttaaaatttg
     4021 ctaatacatg ccccaccatg gcgccagcag ttggtttatg atggtcgata tctgcttgtt
     4081 ttgtttcttg aacaaatttt tcttccacta tattcattga tttacgcctc ttttactttt
     4141 gctgattgga cttcataacc taatttttcc acaactgcag ctaattgttc agggtggact
     4201 tcatctggat caaagtttgc tttgattttg ctcgcattaa acaaaacttt cgtattgctt
     4261 accccaggct gttgagaaac cgcatgttcg attttttgca tacagcttgg acaagctagt
     4321 gtatctaatt gcataataat ttttttcatc gtagttcttc cttccttttt cttgatagtc
     4381 ttattctaga ttacagcttc cataaaaaaa ttgattctta tcaagtttta cgatttttct
     4441 tctttgcttt cgtgtccaaa atacaataaa cgcatcgcgt taaagataac gatcaagata
     4501 ctagcttcgt gaacgaacat cccactagcc atgtaaataa acccggcaaa taagccgttg
     4561 agtaaaaata agacgacacc aatggcaatc actacatttt cacgggtatt taaaacagtt
     4621 ttcttagcca agcggtaggc atgaaccaac gattcaaaat tagattgcat caagacaacg
     4681 tcactcgttt cgatagcaac atccgttcct gatcccatag caatcccgat atcagccgca
     4741 gccaaagagg gagaatcatt gattccatct ccaataaaag caacgcgcaa tccttgttct
     4801 tggtattttt tcacaaattg aaccttttcg tctggtaata attcagcatg gacttcatca
     4861 atgcctaaca tatcagctac ataatcagca gtcatctgat tatcacctgt cagcatcact
     4921 aactgttttg ctcctgcttg tttcaactta gctaattgtt cagcgacttc tggtcgaatc
     4981 acatctgaca cacctatgat ttgagtgatt tttttgtcga ttgctacaat aatgaccgag
     5041 ctgcctgttt gttgtaattg atgtaaatct tgtacttgct ttggtgtgag agaaagtttg
     5101 tgagcggcaa tcaatttggt gttgcccgca caaactgtat gaccatcaat ctcagctgtg
     5161 attccttgtc ccttgattgt ctgattatct ttgactgcta attgttgaaa atcaatgtct
     5221 ttctcctgag cataatggat cacggcgcgc cccagaggat gatcggataa cgtttctaat
     5281 cgagccacta aagctaagct gtccccttca tcctctgtat agttcttgat agtggataca
     5341 gcagtagttc ctttggtcaa tgtaccagtt ttatcaaaga caaaagtatc gattttggaa
     5401 aaagtatcca ttgcttcacc gcctttgact aagatgcccc gtttcgctcc attaccgatt
     5461 cctgctacat tcgaaacagg cgcaccaatg actaaagcgc caggacaacc aagaaccaaa
     5521 acagtgattg ctaaacgaaa atcacgacta atcagaccaa caaccaacgc aataagtaaa
     5581 acggttggag tataatactg agaaaatcga tcaatgaatt tttccgcatg ggatttggta
     5641 tcttgtgctt cttctactaa ttcgataatt ttggcaaagg tcgtatcatc tccgacctta
     5701 gttgcttcaa tcgtcacgta gccatctgct aaaatcgttc cagagaagac ttcatctcca
     5761 ctgattttga cgacttgtcg tgattcacca gtcactgcgg cttcatccgc ataccctttc
     5821 ccgtcaatga ctcgtccgtc cacaggaata gctccaccag tttttactaa gacgatatcg
     5881 ccttcttcca cgtcatcgat ttccacttct tcagtcgttc catcttcttg gacaacacta
     5941 gcagtagatg gggccatctc ggtcaaagcc ttgattgact cccgcgtttt ggtcaatgtt
     6001 ttttgttcca gatagctacc aaacaaaaac aagaatgtaa cgatagctga ctccttgtat
     6061 tctccgatca taaaggcacc aagcacagca atagtaacga gtaaatcaat tgagataact
     6121 ttgactttca ctgcctgata cgcatggatc ataataggta tgaccgcaat aatagaagct
     6181 atggctagag caatgttata gaacaagaca aaaccagtaa attttccgat aaaaccaaaa
     6241 ataatcaaca cagctgataa ggcggtgata tgatttcgat gctcttggat ccacttttgt
     6301 aatttcatct ttctccctct tttcctttca ttcttacgag actaagtata gatacttatc
     6361 catcataaaa aattgaccac tatcaagttt agtcaaaaag gcggttttct tttggtagaa
     6421 tacactacaa aaaatagtta gcaagaagac tcactaaaag tgatcttgcc aactattgtc
     6481 gtttctatcg aaattttcat tgaatgattt tcctgtacag catacatcgg agatctattt
     6541 ctttatctaa gatttttatc ttgctaattt tttcctttag tctttcaaat ccacgtcgtt
     6601 catagaattg atacgtacag ccactatctg taaaaaggaa tatttctttt cctttctctc
     6661 ttttttcaaa ttctgttagc aacttggtac cgatgccttt tgttttcaaa tcaggatttg
     6721 cagctaaaaa aatgatctct ccatctggcg aatgcttctt taaatatgct gcaagcattt
     6781 gttcatttgc tgtctcgtaa gctcctttac tttctttagc aaatatattt tgtaaaaaat
     6841 cgaatatctt aacatatatt tgctgtccaa aagaacgatg tttctttttt tctcctttta
     6901 tctccgccag caaaacacct gccaactcgt cctctgcata caaagcaaga atttgagttg
     6961 ctcgattcaa ctccatatac aaaaaatatc ttccgtaaag attcagcaga agttcatttt
     7021 ccatatatct ctcaaagttc attccttcaa cagcaaaata gatagctttt ttgtaatctt
     7081 tcttttctaa gggtttaaaa gttagtttca tcatttcgcc tcctcaactt caatgattgt
     7141 gttatactta cattataata gacacagtgt ctactatcta taatcaatca tctataagtt
     7201 tgtcgattaa gcgacagctt tgctttgata tgtcggataa ggagataggt aaatgaaaaa
     7261 gacagataga cgcgtcaaaa aaacagaaaa agctttggct gaaacactat ccacattatt
     7321 agtaaataaa aagattcaag caatcactat acgcgaacta acagaaacgg cagatgtaca
     7381 tcgttcgact ttttatacac attataagga tatctatgat ctatatgatc aattagaaag
     7441 caactttttt cgggatctaa atgaaattct ctcttatgat ccagcccata gctatgagga
     7501 actatacact cgcttgattg actatgttta cgcaaatact tttttatatc gcttatttat
     7561 cctcaaccat ggtgatattt cgtttcagaa gaaagttaaa caagttattg aagaaaacta
     7621 tctgaacata tggctatatg aagacaagaa aacgattatc acagaagaaa tgagattctt
     7681 tactacgtac cacgtccaag gttgtctatc cgttctcaat cggtgggtag aaagcaatta
     7741 caagtactcc aaaaaagaat tattagaact cttacagaaa ctgaatgatc acatagagaa
     7801 aatcatgcct tgaataaaaa catactaagc ttagttaaga agaaccgtaa ccttctcgta
     7861 taacaactga atcttattta gccaaattgt ttactgaaga ttttttgagt aaaatttcta
     7921 taattcctac ttattgaaaa gtagtttgta gtgctgcttc tccctctcta agtgatatat
     7981 cttgttgtac caatcaaatt cacctcatca ttaataaatt acttttaggc taatttatta
     8041 caacttatag gttgaagctg taaccaattt atagtcatat tatattttta tcatccagtt
     8101 tattgaattc ttctcgttta cctcaattca ttttcaaggg gaatca
//
LOCUS       DAYYME010000118         8060 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000118 DAYYME010000000
VERSION     DAYYME010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.119"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACHBFW_002290"
     CDS             476..1339
                     /locus_tag="ACHBFW_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9318560.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACHBFW_002291"
     CDS             complement(1468..3090)
                     /locus_tag="ACHBFW_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9318561.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACHBFW_002292"
     CDS             3339..3860
                     /locus_tag="ACHBFW_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM9318562.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHBFW_002293"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHBFW_002293"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFM9318563.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACHBFW_002294"
     CDS             5941..6759
                     /locus_tag="ACHBFW_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFM9318564.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACHBFW_002295"
     CDS             complement(6862..7059)
                     /locus_tag="ACHBFW_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318565.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACHBFW_002296"
     CDS             7228..7806
                     /locus_tag="ACHBFW_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM9318566.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACHBFW_002297"
     CDS             7901..8038
                     /locus_tag="ACHBFW_002297"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318567.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2406 a         1467 c         1593 g         2594 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt
//
LOCUS       DAYYME010000119         7897 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000119 DAYYME010000000
VERSION     DAYYME010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.120"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            242..2008
                     /locus_tag="ACHBFW_002298"
     CDS             242..2008
                     /locus_tag="ACHBFW_002298"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFM9318568.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2110..3369
                     /locus_tag="ACHBFW_002299"
     CDS             2110..3369
                     /locus_tag="ACHBFW_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9318569.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3516..4817
                     /locus_tag="ACHBFW_002300"
     CDS             3516..4817
                     /locus_tag="ACHBFW_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9318570.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4820..5671
                     /locus_tag="ACHBFW_002301"
     CDS             4820..5671
                     /locus_tag="ACHBFW_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM9318571.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5684..6499
                     /locus_tag="ACHBFW_002302"
     CDS             5684..6499
                     /locus_tag="ACHBFW_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFM9318572.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGVSFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6650..7816
                     /locus_tag="ACHBFW_002303"
     CDS             6650..7816
                     /locus_tag="ACHBFW_002303"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9318573.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         2582 a         1362 c         1580 g         2373 t
ORIGIN      
        1 ttaaaaaact tttactttag aaaattggag aaattgatcg cttaagataa attcaggaca
       61 ctaaatagta caggaagtag actaaaactt ttaaaagttt taagtctatc tatttggcaa
      121 gtttaaaaaa taaaaattcg gtatcaaaaa actgttaccg ataacatttt tgaaagcgtt
      181 gtcttttgct tctctatcac ttatagtgat gacgtcatat taaaacacaa ggaggaaatc
      241 aatggataca gctgcaattt atcatcgacc ggaaagtgaa tatgcatttc tatatacgaa
      301 tacaagattt cgtattcggc ttcgtactag gaaaaatgat atcaaaaaag tatatgtgct
      361 atgtggagat ccatatacca taacaactga aaaatggtat caaaaacaac gaccaatgaa
      421 aaaatgtcta agtaccaatg ttcatgatta ttgggaaatt gaagtgacga gcgaaacaag
      481 tcgcttacag tatgcgttcc atgttgttgg ggaagataac acggactgtt tttacggtga
      541 tcaaggaatt tttccttatc gagaaaatgt gattactgaa ccaaattttt attttcgaat
      601 accttatttt catcaaatcg atcgattcac tattccagaa tgggtaaaag aaacagtatg
      661 gtatcaaatt tttcccgaaa gatttgcaaa cggggacaaa gcaaatgacc ccgaacacac
      721 tttaccttgg ggaagtaaag atccagaccg agaagacttt ttcgggggag atttgcaagg
      781 tgtgatggat catttggatt acttgacgga ccttggcatc aatggcatct atttttgtcc
      841 catatttaaa gccacatcca atcataaata cgatacgatt gattattatg aggtggatcc
      901 cgcttttggc gataaaaaac ttttaaaaaa cttgattgat gaagcacata aaagagggat
      961 acgtatcatg ttcgatgctg tattcaatca tatgggtgtc cattcaccac agtggcaaga
     1021 cgttctcaaa aacggagaaa aatcgatcta taaagactgg ttccacatcc gttttttccc
     1081 agtagattct tatcagatga ctgacttacc agaaactgct gaaaatatcc catatgatac
     1141 ttttgctttt actccattca tgccgaaact aaatacagca aatccagaag tgcaaaaata
     1201 tttgctggat atcgcaactt attggataaa agaattcgat attgatggat ggcgtcttga
     1261 tgttgctaat gaagtcgatc atcatttttg gaaaaaattc agagaagcag ttacagaaat
     1321 caaaccagac atctatatcc ttggagagat ttggcattcc tctcaagctt ggctgcaagg
     1381 ggatgaattc catgcagtga tgaattatgc atttacagat tcgattaaag attattttgc
     1441 aaaaaagaaa atcacagctt cacaaatggt cagtggaatg aatcatcagc aaatgcttta
     1501 cagagatcag gtgaacgaag gaacattcaa cttattagat tctcatgata ctgccaggat
     1561 cttgaccctt tgccaaggta acaaagaatt gatgaagtct gtgatggcct ttatgtttct
     1621 tcaaaaaggg tctccttgta tctattacgg gacagaaata gggatgactg gtgaagatga
     1681 cccggattgc cgcaaatgta tgatatggga gaaggaggaa caagatctag agctattcgg
     1741 ttttataaaa gaacttgttt ctctaaggaa acagttgtct aagattattt ctgaaggttc
     1801 tactcagtgg ctaatagtaa atgatcgtga agataagctc tatttcacac ggaaactaga
     1861 gggacagatc atctatgttt actttaatca gtcaaaagaa ccttgggtag tagaacagga
     1921 aaatgaggtg attcttagtc aaaattgtca gttgttagaa gacgggaaag cagagatacg
     1981 acaatacgga tttttgatca tgtgttgaaa actgttaccg gtaacaacaa gaaagcgttt
     2041 gcaaatgaag atttgtgccc attataataa gggtgtaaca gcacgttgaa tacaaaggag
     2101 gaaatgaaga tgaaaaaaag aagtgtgaaa cagttgggtt tagggttaat ctctttagga
     2161 atcttaggcg gattagcggc ttgtggcaat ggtaactctt cagcagaagg ggaagccgat
     2221 gaaggaaaaa cgttaacagt ttccgttgat tctggatata aagattatat aaacgaaata
     2281 aaaaacactt tcgaaaaaga aaacgatgtg aaaatcaagc tagtagaaaa ggacatgttc
     2341 gatcagttgg aatcattagc gttagatggg cctgctggaa agggacctga cgttatgatg
     2401 gcagcatatg accggatcgg tgcgttagga cagcaaggac atctggcgga agtaaaactg
     2461 ggaaatgaag cagcttacga tgaaacagac aaagcccaag tgacttatga tggaaaaatc
     2521 tacggagaac ctgcagtaat cgagaccctt gttttgtact ataacaaaga tttagtcgat
     2581 acagcaccat caacgtttaa agatttggag aatctatcca aagacagtcg ttttgctttt
     2641 gagtcagaag caggaaaaaa tacaggattt ttagctaaat ggactgactt ttattattcc
     2701 tatggtttaa ttgcaggtta cggtggctat gtatttggtg atgatggcac aaatccttca
     2761 gatatcggtt tgaataatgc aggtgctgta gaaggaatct cttatgcaac agattggttc
     2821 aaaaatgttt ggccaaaggg aatgcaagac ataaaaagtg caggagactt cgccagccag
     2881 caattcatgt ccaataaaac tgcagcaatc attgacggtc catggcaagc gcaaacatac
     2941 aaagaaaaca atatcaatta tggtgtagcc aaaattccta ctctaaataa tggacagcct
     3001 tatcaaccgt ttggcggagg aaaaggatgg gtagtcagca attatgctaa aaacaaagac
     3061 ctctctcaaa aatggcttga ttatgtaacg aatcaggaaa atcaagaaaa attctttgaa
     3121 atggtaaatg aaatccctgc aaaccaacaa gctagggaaa cagcaaaggc taaaaacgat
     3181 gaattaacaa cagctgtgat tgaacaatat gaaacagctc aagcgatgcc aaacatccca
     3241 gaaatgggcg aagtttggac aggtgcagaa aacttgatgt tcgatgcagc atcaggttca
     3301 aaaacaccaa aagaatccgc agatgaagca gtaaaaacaa tcagtgaagc catagaacaa
     3361 aaatactgat taaaatgata accgtccatt tttataaaaa atggaacagg tgcttcagat
     3421 aaaagtggca agagcgagat ggaaaattat cttgctcttg ttgccattat cttaaaagca
     3481 gtttgcggtt tagcaagttt agagggggca ttcagatgca gcaagataaa acaagcaata
     3541 tcaacgaaaa aaaaatcacg ctttgttcac ttattccagg gatggggcag tttgtaaatg
     3601 gccaagcttt taaaggtatc gtttttttcg ccatattcgt cctattcatt ggtcagatgc
     3661 ttctgggcgg atttcatgca ttggtaggat tggttacttt gggaagtata ccaatggaag
     3721 atcattcatt atttattttg attgaaggca ctttgcaatt catcattaca atcattttct
     3781 tgatttttta cggtttgaat cttttagacg cttatcgagt agcgaagatg cgtcaagagg
     3841 gcaaaaaaat caatcggacg atgaaggaag tcatacataa tataggtgat gaaggttttc
     3901 cttatctatt gacacttccc gcttatcttc tcatgatttt tacgatcatt ttcccagtcc
     3961 tagttacatt gttcactgca tttactaatt atgacttcaa tcatattcca ccagccagtt
     4021 taattgattg ggtaggggct aaaaactttt ttagtatctt ctttttaagc tcatatcgta
     4081 atactttttt tgcagtcttt tcgtggaccg tgatttggac tttatgtgca acaactttgc
     4141 aaatcaccct tggtattctc acagcggttg ttgctcacca gaaatttatt aaattcaaac
     4201 gtattttcgg tatcattttc ttattaccat gggcggtccc agcttttatt actatcatga
     4261 gtttttcgaa tatattcaac gatagcgtag gtgccatcaa tactcaagtg attcctttca
     4321 taaaccagct tccttttgta gatatcagtg caatctcttg gaaaacggac ccgaactgga
     4381 cgaaagtagc aattatctta gtgcagggct ggttaggatt tccctatatc tatgtgatgg
     4441 ttacaggaat cttacaatcg atttcagaag acttatatga agcagcaaaa atcgacggag
     4501 caaatgcatg gcagcgcttt tcaaacatca cgcttcctgc tgtcttccta gtggcagcgc
     4561 caacatttat tacacagtat acggggaatt tcaataattt ctccatgatc tatctgttta
     4621 acaatggcgg tcccggaagc gtaggcggag gagctggttc tactgatatt cttatttcct
     4681 ggatctataa acttacgact ggaacttctc cgcagtattc tatggcttca gcaatcacgt
     4741 tgattatctc cgtcatcgtt atctgtgtgt cattgctggt atttaaaaag acaaatgcgt
     4801 tcaatatggg ggattaacaa tgaaaaaatt tcattcgagt gctcgattcc aaaaacgact
     4861 ttctcacttt tttacttact tttttctttt aatactgtca gtcattatca tctatccgct
     4921 tttgatcaca gcaagtactg ccttcaagtc aggaaatatt gcagctttta gtttagatct
     4981 ttcaagtgac tggactttag gaaactttac tcggctattc agtgaaacac tttacgggac
     5041 ttggtataaa aatacattga tcatttctgt cctaacaatg atcgtacaag tgactgtagt
     5101 aactttggcg ggctatactt atagtcgcta tcgttttatc ggccgcaaat caagtctgat
     5161 gttcttttta gtgattcaga tggtaccgac tatggcagca ttgactgcct tttatgtcat
     5221 ggctttctta ttagaagcgc tcgataagta ctggttcttg acaatgatct atataggcgg
     5281 tggaattcca atgaatactt ggctgatgaa aggatacttt gacactgtac caatagattt
     5341 agatgaatct gctaaacttg atggggcagg gcatttcagg atttttttcc agattgtatt
     5401 accgcttgtc aaaccgatgc tcgccgttca agcattatgg gcttttatgg gaccttttgg
     5461 ggatttcatg ttagctaagt tcctgcttcg aacacctgaa aatcagactg ttgctgtcgg
     5521 cttacaaaca tttatttctg atgtgacaaa ccaaagagtc tctctttttg ctgctggggc
     5581 aattttgatt gcactaccga tttgtctatt attcttcttt ttacaaaaga actttgttac
     5641 cggcctgaca tctggcggaa caaaaggata ggagcagagt atcatgcaaa catttccgtt
     5701 aaactatttt tccgcattac gtacgccaac tcaagtgttt gcgggacgca aactattatc
     5761 atggcctaaa tttttcttga tatttgtatt tttagtttcc ttgatggtca tgcctgtgac
     5821 tcttttttat gccaatcaga tccaagcgat tccattagaa caatttctat ccgttcattc
     5881 gctgatcgat gaacaaggaa cccaaaaatt ttctgaactt gaattatctg aaactgggtt
     5941 acaagcatcg caacaaacga tagctgtgac ccctgaaatt ttggtaggtg tgtctttatc
     6001 tgagaaacaa caatccgatc atggaacttt tattgatttt gagaaggaac aatgggtgat
     6061 ccaacaaaag gataaatctg gtatacgccg ctatacgatg aattatagcc catctttcca
     6121 accagattct gtacgtacgc ccgaagattt ccaacgattt ttagaaagag aattttatgc
     6181 cagtaatcgt ccaacaatca ttttatctta tagtttaagt cttggtcttg tcctatttgt
     6241 catgacgagt ctcattttat tcggggtttc ctttttcttg tggatgacaa gaaaaagcca
     6301 actttcatcc attcatacat tcaaagagag tgcaaatttg atgctgaatg ttatgggaat
     6361 aggttccatg gcagcagcag ttgtagggtt tatccacttt gactttatct tgatgctaag
     6421 tgtccaaaca acgataactg tcttgctgct tttatgggtt tttgtcaaaa caagattcaa
     6481 agataagcgt gtcgaatgac aagtttaagc gatttatgcg acaatgaatg tgaaaaaaat
     6541 gaaaaataaa gtagtttttg aaaaatttct gaaaaaatta aacttcttag ttgattcttt
     6601 tcttttttag tattaacgta atagggtagt ttttttaagg aggaaaatca tgttagagaa
     6661 aactttatac aatcaattat tttcacgttc tttctcgctc cctgtcgaag taaattattg
     6721 ggacggtaca acgaaacaat acggagatac ggacactcct ccccaaatca aaatcaatat
     6781 ccatgaagaa atcccaatga aagagattac aagtaacgca tctttggcac taggagaagc
     6841 ctatatggat catcgaatcg agatcgaggg aagtatccaa gcattgatca acgatgctta
     6901 tcagcaagca aacagctttt tacgaggaaa agattatcta aaatggctgc caaagaaaga
     6961 aaaacatacg aaacaaaaaa acaaagaaga tatccatgcg cattatgact taggtaacga
     7021 cttttataaa ctttggctag atccaacttt gacttattct tgtgcttatt tcaatacacc
     7081 tgaagatacg ttggaacaag cacaaatcaa taaagtccat catatcttag acaaactctt
     7141 tatcaaagaa ggcgaaacgt tgcttgatat cggttgtggc tggggaacat tgatgttcac
     7201 tgctaccaaa gagtataatg tcaaggcaac tgggatcaca ctaagtgaag aacaatacga
     7261 tttgattacc aaaagaataa aagaagaaca tttagaagat aaatgccgtg tattattaat
     7321 ggattaccgc gaactaaaag gcgaaacgtt tgatcatatc acaagtgtag gtatgttcga
     7381 acatgtaggt tctgaaaatc ttgaaggtta tttcaaagtg gtaaaagatt tattgaaacc
     7441 aaaaggaaca gctttgatcc atggaatcag ccgacaacaa ggcggtgcga caaatgcttg
     7501 gatcaataaa tatattttcc ctggcggtta tgtccctggt gttactgaat tagttgataa
     7561 tattacaaaa aacgatttgc aactgattga tttggaaagc ttacgtcgtg actaccaatt
     7621 gacattagaa cattggacaa agaattttca tcaagtccaa gatcaagtca cgaaagaaat
     7681 gggtgattcc ttctatcgaa tgtgggatct atatttacaa gcttgtgcag cttcatttga
     7741 atcaagtaat atcgatgtga ttcaatattt gcttgttcaa cctggaaaca atgacatccc
     7801 aatgcatcgt gcataattga ggcaaaagta ctgtttaaag actatttaaa aagaagctaa
     7861 gttttatgat gctccagaag tgaacttttt tgtctaa
//
LOCUS       DAYYME010000120         7807 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000120 DAYYME010000000
VERSION     DAYYME010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.121"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1379)
                     /gene="celB"
                     /locus_tag="ACHBFW_002304"
     CDS             complement(27..1379)
                     /gene="celB"
                     /locus_tag="ACHBFW_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM9318574.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(1379..1897)
                     /locus_tag="ACHBFW_002305"
     CDS             complement(1379..1897)
                     /locus_tag="ACHBFW_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318575.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1913..2239)
                     /locus_tag="ACHBFW_002306"
     CDS             complement(1913..2239)
                     /locus_tag="ACHBFW_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFM9318576.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(2252..4231)
                     /locus_tag="ACHBFW_002307"
     CDS             complement(2252..4231)
                     /locus_tag="ACHBFW_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9318577.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(4252..4566)
                     /locus_tag="ACHBFW_002308"
     CDS             complement(4252..4566)
                     /locus_tag="ACHBFW_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFM9318578.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4734..5027
                     /locus_tag="ACHBFW_002309"
     CDS             4734..5027
                     /locus_tag="ACHBFW_002309"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFM9318579.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(5105..6217)
                     /locus_tag="ACHBFW_002310"
     CDS             complement(5105..6217)
                     /locus_tag="ACHBFW_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM9318580.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            6370..7218
                     /locus_tag="ACHBFW_002311"
                     /pseudo
     CDS             6370..7218
                     /locus_tag="ACHBFW_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7571..>7807)
                     /locus_tag="ACHBFW_002312"
     CDS             complement(7571..>7807)
                     /locus_tag="ACHBFW_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM9318581.1"
                     /translation="DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKH
                     LPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         2495 a         1554 c         1262 g         2496 t
ORIGIN      
        1 tgtcacagtt ccttttttct tagaaatcat tttaactttt cactagctaa ttctgctgcc
       61 ttttctcttt cttcatttaa taatttatta tcatagaatt tgataaatgg gaaccagata
      121 atgaatgcga tgattgcaca aatgactgct aacacagcag ctttccaatc accaccacta
      181 ccaacaaatg ctccgatccc gactggtgaa ggccacggca tctgtgcaag catcggtttg
      241 acgatttcca gccgaatggc aaaataagcg agtgtagcag aagccatcgg agctaagaaa
      301 aacggaaccg ctaaataggg attgtagacg attggcatcc cgaaaatgat tggttcattg
      361 atattgaata atcctggtac aagtgaggct tttcctaaca ctttcagctg atcagatttc
      421 gcgaggaatg caataaagat gatcaatcct aaagtcgcac ctgatccacc aacggttaca
      481 taagaattgt tgaattctcc tgccaacgga taatttgcgc cttgtgcgtt agctgccata
      541 ttagctaata cgatcggggt caagaaagaa gtgatgatat tcgctccatg gatccctacg
      601 atccacaatg catggatcaa gaaataaatc accatgacac caagccatgt attcgtcaac
      661 tgcgtaacaa acccaaatgg gatagcaatc attttgaaga tatccgttcc tagtgcaact
      721 aataatccat tgatgatcat caccacaaac gcaatgacaa atgttgggat cagagcagtg
      781 aaagaacgag aaactcctgg cggaacaaca tctggcattt ttatgaccca atttctcttg
      841 acacacatac aatacaattc gaccgcaata atcgacataa taatagctgt aaagattcct
      901 gaagttccta aacgttcgac aaaagagccc atcctaatac catttactac agtttctgtc
      961 tcattgatag aagcaatcaa gctgatttta ccatcagtca tgatcaattc agggatacac
     1021 ataaagaatg caaaaacaga tagtaatgcc cccgtgagcg gattaacatt taatttttgt
     1081 tcatctcgtt caattgacgt taattcataa gcaaacacaa tggcaaagta aatagataaa
     1141 atccccatcg tcattgtatt gacgatcatg tacaagtcac taactttgaa aaaagttgca
     1201 ttaaaaaatc cttctaaaaa tgggaacggt aatggcaaaa tatttaaaac caaaaacatc
     1261 gagcctacaa tcgtgaaagg aatagaagcc atccccgctg ccatgacagc ccgaacgatt
     1321 ttaagttgcg caattttccc cataggtccc atcaggtatt tctgcaaaaa atcaaacatt
     1381 agttattctc ctttcctaaa tataacgctt tttcatattg tttaatacgg atatactgtt
     1441 ggtcttggtt gagtgaaatt ttctttaaac gatactgtat aaatgcactg gcaaaaatac
     1501 cgcttacaac aagaatgaaa acaaataact gtcctccatt gttatttgcc ataaaaggat
     1561 acaagaatgt aaatcctgag gtaaacaaca ctggaatcac taataaatta aagacactct
     1621 ggaacaaaaa ataccacttt gtccatggaa cgttcgtttg gtgttttcga taaagcttta
     1681 cttgttctcc tactgcaggc aaaatagcta aaatcagaat catcggtaaa ctacttccta
     1741 aacttttagc tgatagaagc agaatcatcc attgcatgtt catgaaaaag aaaaaggcag
     1801 tcaggtaacg tacgattaga taacgattga aatacatgtt tttcaaacta agttgctttt
     1861 tttctaattc gattttcctt tttactgtat cctgcacaat gatccttcct ttctatttat
     1921 ttttttgata caaataattc atttcctgtg caacttccaa taatgtcatc gtcgtcatca
     1981 gatgatcttg tgcatggacc atgataattt ccatttctat ttttgtcccg ctggcatatt
     2041 ctttcagaag ttgagtttgt gactgatgcg catgaagaat ctcttcattt gcttgtcgca
     2101 acttctcttc tacttcttca aacatctctt tacgcatcga gcgaaatgct tcatgtatca
     2161 gtgtccgtgc atttccgcta tggaaaatga tatcaaaaga agtcacttgt agtgtttcac
     2221 tgtttatttt ttcagccatc tgttttcctc cttattcgat cagtttgata aatttactgc
     2281 tgaaattctc aaaaataggt tcagctaaga tttcctgctg tacttttagc tggtcaacta
     2341 acctaacgat tgctttagtc acgaccgtaa ttccttcatt ttcgacataa gaaggcgaaa
     2401 ctaaaaaaac aaaatgaatt tcttgatatt gatcccactg catcccagaa ggaatcaatg
     2461 caacggctac ctttgtttcg attccaactg gaacagcggg atgtgggact gccactgtat
     2521 cactgaatat gatttgtccc atattttcac gttgctggat ctgctttcgc atttctctta
     2581 tatatccttc ttcttcattt atgctcagct tttgcagcaa atcatgaaga atttcttcct
     2641 ttttcggttc gtacgtataa gtcttgaaaa atttttcaga caactgttcg gtgtaaatct
     2701 gctttttttg ctgtagagaa aatgctggta tcggtctatc tgaggaatga tagggtatag
     2761 attctccaac tacctttcgt attttacgaa catcatcttc attcaaaaaa atactgacat
     2821 gtaaaactgg aattttgaaa aacattgtcg aaagatcaat agaagaaata atcaggtcga
     2881 ttccttttaa agttgtttca ttgatctcat aatagccttt tacacttacc actgtaatat
     2941 ttttaccgaa ttcactgact acgcgattct ttaatagttg ggcactgccg taacccgttg
     3001 cacagataat caatgcatgt agtttatgcg cagctctttc tttttccaat gccgccatga
     3061 gatgcaaagc caaataggcc cattcatctt cattgatggt atagtttttc aaagccggca
     3121 tgttgctgaa atgccttttt actgcgtcaa agactttggg attttctttc tttatctcct
     3181 cagtcaatgg gttctcaatg cgaatacctc ggctcaatcg aatcatcatc ggtgacaaat
     3241 gatcgagtag tccattgaaa agctggagat cctctactaa tgaaaaatga aattcctgag
     3301 acaatctttt cagaactttc gctaattcct ttgatagttc ctgatcattt tgattttctt
     3361 tgtgattcga cttagccatg agatgtaatg tcaaataagc aatttcttct tcgggaaacg
     3421 atacacctgc gattacctca atacgttgaa cgatccgctg cgccacttga tactctactc
     3481 gcttagaaac ttcttgttcg atgcctaatt ccttgatttc aaacccttct cggagccgtt
     3541 tgatccccaa agccaagtgt aaagtcagat tctgtatgat aaaatccgaa acttttaact
     3601 tagcttctct tgtttcatcg agaatgataa tgactagctc ttcaaaactg atgtctggga
     3661 aaaaacggct ttgccctaaa tattctttca atgaatttcc ataatggttt ccaaaaaaag
     3721 tatccatgat aaaatgccgt cgatttcttt cttccccttc gatccatgtc ccttgaccat
     3781 gcttagaaac gatttttagt gaatatggaa tcaatcgctc cttgatttct tgcatgtcct
     3841 ttgtcaaact ggaacgactg atataaagct cttctgccaa atcatccata aaaagaatgg
     3901 catcttccaa aagcaattgg ttcaatatat acttttgtcg atcttcggac tcataaaatc
     3961 cttgcggttc tctagcatat ccaggtacta tttcttgccg actcaaaaac aagtcaaatg
     4021 tcagtctttg taaaatatgc agttggtagc cataacctgg tttagcaatg atcttacctc
     4081 cgtttttttc tactaactct ttgatccgct gcaagtaatt acggattgtc cgatcagata
     4141 ataacaattc agaagctaaa cgttgactgg tgacatactc cttcttgtga tcaatcaaat
     4201 atctcaacag caatttttcc ttctgtttca ttatcatcca ctcctattcc atcatatgtt
     4261 ctcttcgatc acctctgcta acttctggat gcctgtaggg attgggacat acgcttggaa
     4321 agggatacta acaacaggct ttccaacaga ttctcctaat tttttgaatt tatccaaaaa
     4381 catcgtcgtt tgcgggctga tcagaaataa atcaaaatca ctattcttaa tcatattatc
     4441 gccttctgta gctgaaactg catctacgag tacctcttct cttttttgct taaagaaatc
     4501 cgttgtcttt gaggccatca tcgaggatga cattcctgcc gcacaaataa tcaatgcttt
     4561 tttcataaat caatcactcc ttttattttt ataaaccaag catatcttgt aagcgttttc
     4621 tattcaaaat atatatttcc gtagcattgc ggaaaaaatg tgtatcatat cttgaataat
     4681 aatttctggc accgtgatat actagtgata gaattttttc aggaggggat ttagtgagta
     4741 tcacagtgaa tattttgtat acaagtacaa atggacaagc cagaaaattt gcagaagaaa
     4801 tggtcgaaag aaacattgtc aaagcgattc gtgcagaaga aggaaacgag aaatatgact
     4861 attactttcc actagaagat ccagaatctc ttttactgat cgaccgttgg aaagatgaag
     4921 aagcaatcga aaagcatcac aaatccgaaa tgatggctca aataaacgaa ctccgtaaaa
     4981 agtataaatt aaaaatgaaa gtcgaacgtt tcactgattt accgtaaaaa gatacacatg
     5041 gaatgatagc aatttatcat ccatgtgtat cttcactttt tccttatttt ttattcgatc
     5101 tcatctaatg attcaaaccg tgtttttctc ttatttttcg gataagtatt tcagtcaccc
     5161 caatatacat gattcctaaa cataccccaa ggatattctc aaaaatgcgg atcgttactg
     5221 ctcctggaac cccgaataaa ctggcagtga ttgccaaagc accaaaagaa ttaaaaactg
     5281 ttttatatct ataggtagaa caaacaccta aagctagacc acctaatata ccgacccagt
     5341 taaaaggaat aaattggctg ataccaataa ataaaacaca tccaatcaat gagccaatga
     5401 ttcggtctac tgctcgttct ttgaaaccaa tcgacatcaa tccatagcta gatacaatag
     5461 aagaaaatgc aaaccctgcc cacatcaatc gctgaaaagg gacatactct cctgcaacga
     5521 tcaaaagact aatgccaaat gcatagatga gcatccaaat tttttcctgc gaatacatgc
     5581 ctttcaaaaa aatctcttta aacaagcttt tgtctttgtt cttttctcga tgttttctgt
     5641 aaaggagaac tgaaaaccaa caaaaaaaca aaaataataa gctacttgtt tgtgtaaaga
     5701 aattcttgtt caacgaatct ttaggtaacg aataaacaat aaagagatat gaaaagccgt
     5761 acaaagaagc attccccatt ttgggatcac tagcggttgc cattaatatg ctgcttagta
     5821 aaataaaatg aatcaacatg cccataactg accatttgat cagctggaca tacggtgcta
     5881 ctaatagtga gaacatgacg attgctaaac ctatgatcga atgggaaatc ttataaccaa
     5941 agtcaacaaa acgaatactc atcaacatac agaataaaac cacagcgtat ggtttatgct
     6001 tcgattcaaa taatgttgta attataatga taaaacaaat ggcaaacaat acgatgaata
     6061 atgatcgtaa aaacaatgaa caaatataca tgcacttttc ttttttagaa gctgcttctc
     6121 gtattttttg ttttaaaata aacggatcta gttgtagaga ctgataaaaa tccacctgtt
     6181 gtcgtccttt ctttttactt ttatttttta cagtcatcta tgacaggttg taaatactat
     6241 ggaacatcat atggcgttgt cattttcttg tcaactattt ttcctattca tgtttttata
     6301 aaaaaacata tcgttttctt gtatgattaa atcaccacaa aataaacaac tgagagaacg
     6361 atatgcttta tgaatggttc tatcaaaaaa attctgagat caatagacaa aaatttgatg
     6421 attacagagg tctcttacga aaccttacag aagaaaaaaa cattgatcgt tgctgcagtt
     6481 ctcttccctg ctccatgtgt ctgtagaaat tgtggttctg ctgtggtaga tggaaatagg
     6541 aaattaatgg tggtgaaaaa caagaagaag gaaaccattg tccactttga ataatataat
     6601 catatgccaa tggttatacg cctaaaaaag cagcgctata cctgtaaaaa ctgtcgaagc
     6661 cattggaacg ctcaaagtta ttttatccgt ccaaggcact cgatttcaaa tcatgtcaga
     6721 cataaaatca cttctttact gactgaaaaa gtgtcgttat tttttatttc gaaaagttgt
     6781 taggtatctt tgacaaccgt tattcgcaca ttgaaagagt tgagaagcta tttaccaaag
     6841 tcttccaaaa gaattctgtc ttgagtgttg agggttgtga atttcgttct cacgtttccg
     6901 ttgaggataa actgagtttt atttccgcgg atggcgagac agaaaaatta atcgagaaaa
     6961 tttacctaca caaaaaattc ctcgattaac aaactatttc cttagctgca ctaatcctaa
     7021 agaaatagaa ttcttagtta cagacatgaa cgccacctac tttcagctca ccaaacatgt
     7081 tctaccaaat gcgaaattgg tgattgatcg attacttggt ttttccacgc ctttcaagga
     7141 ggcgtaacct ttattcacga gttggttgaa gctttccgag acaaaagacc cgacttgttt
     7201 ttctccttat tgacaaagag ccattaaaac tagagaaaaa attacaactg atcgctgatt
     7261 atttgtagaa agaaaatcaa agtattaccc aataaacttg agtcattagg caaaaaacac
     7321 tgcgaaactc aagaatgatt ttcgcagtgt ttttgtatat tttttattaa ctagtattac
     7381 tacaaaggtt tatttttcac ttctcaacgc agcacaaaat tgtctttact tatttactgg
     7441 ttgggttcaa ccttctctac tccgatacta atatttgttt gtatacccct tctaaaatcg
     7501 gtagtttcca ttcaattgat gggtactagt tgattttaaa aactatgctt attcaatttt
     7561 ttattttagc tcatatattt cgtttctttc taaattttat actgatgaaa acaagtagca
     7621 cacccaatat tgacaatata aactggctaa tgatggtttc tccagttttt ggaagatgct
     7681 tatttccact gcttgttgga ttgtttcctc ttatatctga gctatcagca acttttctat
     7741 tctcttttgt taaaaagatc gccacttctt gttctttttc gatcttgaaa gttactggtg
     7801 tcgcatc
//
LOCUS       DAYYME010000121         7794 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000121 DAYYME010000000
VERSION     DAYYME010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.122"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            142..1065
                     /locus_tag="ACHBFW_002313"
     CDS             142..1065
                     /locus_tag="ACHBFW_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFM9318582.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1071..2324
                     /locus_tag="ACHBFW_002314"
     CDS             1071..2324
                     /locus_tag="ACHBFW_002314"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFM9318583.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            2458..3804
                     /gene="gor"
                     /locus_tag="ACHBFW_002315"
     CDS             2458..3804
                     /gene="gor"
                     /locus_tag="ACHBFW_002315"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFM9318584.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGIGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(4618..5571)
                     /gene="menA"
                     /locus_tag="ACHBFW_002316"
     CDS             complement(4618..5571)
                     /gene="menA"
                     /locus_tag="ACHBFW_002316"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFM9318585.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(5584..6687)
                     /locus_tag="ACHBFW_002317"
     CDS             complement(5584..6687)
                     /locus_tag="ACHBFW_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM9318586.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(6777..7712)
                     /gene="pplA"
                     /locus_tag="ACHBFW_002318"
     CDS             complement(6777..7712)
                     /gene="pplA"
                     /locus_tag="ACHBFW_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFM9318587.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
BASE COUNT         2455 a         1404 c         1553 g         2382 t
ORIGIN      
        1 tttttatatt tggacagaat atatcgaact ttattttgat tttttgtgaa ataattttca
       61 aaaatccgac tttaaataga agaagtattg gaaagttttt agagctatgc tactattatg
      121 aagttgtagg aggagaaaat tatgatcgaa ctcataacca cagtagaaac tattgaacaa
      181 gcagaacagt tgcttgctgc aggtgcagat acactatatt tcggtgaaga gcaatttgga
      241 ttgcgtctgc ctgcttcatt tacacgagaa gaacagcgac aattggtaga attagcacac
      301 aagtatggta aaaaagcgaa tattgctgta aatgggatca ttcatcctga aaaaatgaaa
      361 aatgtacctg aatatttggc ttttttaaaa gaaataaacg tagatgtgat cacagttggt
      421 gatacaggta ttatttatac gatgaaaaaa aatcctgaat tgcgtatacc gtatatttat
      481 gatgctgaaa cattagtaac gagcgcacgt caaatcaatt tctgggcgaa aaaaggcgca
      541 gttggagcag tacttgctcg tgaagttcct tttgaagaga tgaaggcgat ggaagaaaaa
      601 ctcgatattc ctgtagaaac ccttgtgtat ggtgctacat gcatccatca atccaaacgt
      661 ccactgctac agaattacta taactatacg aaacaagacg aacaaaaaga tcgtgaaaga
      721 ggtcttttta tctcggaacc taaaaaagaa gagacacatt attctattta tgaagatagt
      781 catggcacac atatttttgc cagcaatgat ctgaatctct cgaatgaatt gaatgaactt
      841 tatcagcatc attactgtac atggaaattg gacggattat ttactccagg cgaaaaattc
      901 gtaaacatca ctcgactatt cgccgaagca aaaaaacaaa ttttagaagg tagctgggat
      961 caagaaaaag cggaaaaaat gactagcgaa atcagtaaat tgcatcctga aaaccgtgaa
     1021 ttagatacag gattctttta tattgatcct tctgctatca aataaggagt atgatgaaag
     1081 aaatgacagt aaaaagaaaa ttgaaacgac cagaaatcct agcgccagca ggaacactgg
     1141 aaaagttaaa aaccgcgatc cattatggag cagatgcagt atatattggc gggaatgcat
     1201 atggtttaag aagccgtgca ggtaatttta cttatgaaga aatggctgaa ggagttgctt
     1261 ttgcaaaaga acatgatgcg aaggtatacg ttgctgctaa tatggtcacc catgaaggaa
     1321 atgaagatgg agcaggggaa tttttccgca gtttgcgtga tgtcggtatt tctgcagtga
     1381 ttgtttctga tccagctttg atcgaaattt gtgcgacaga agccccaggt ctgccgatcc
     1441 atttatccac tcaagcttca gcaactaatt ttgagacatt ggaattctgg aaagaagaag
     1501 ggttggaacg tgtcgtttta gctcgtgaag tttcaatgga ggaagtggca gagattcgta
     1561 aaaatacgga taccgaaatc gaagcattta tccatggtgc catgtgtatc tcttattctg
     1621 gtcgatgcac attgtccaac catatgtcga tgcgggatgc aaaccgtggc ggatgttctc
     1681 aatcttgtcg ctggaagtat ggattgttcg atatgccttt tggtcaagaa cgaaacagtt
     1741 taactgatac aggcgaagtg gaagaagaat tctcgatgag tgcagtggat atgtcaatga
     1801 tcgaacatat tccagaattg attgaaaacg gcgtagacag cttcaagatc gaaggaagaa
     1861 tgaaatcgat ccattatgta tctacggtgt caaatgtcta taaagcggct gtagacagtt
     1921 atatggaaga tcctgaaaac tatgtctgca agcaagaatg gatcgacgag ttatggaaag
     1981 tagcacaacg agaattagct acaggttttt actacaatac accaactgaa aacgaacaat
     2041 tattcggtga acgtcgtaaa atcccagtat ataaatttgt cggtgaagta ttatcttatg
     2101 atgaggcaac acagattgct acgatacgtc agagaaactt gttttctgta ggagatgaaa
     2161 tcgaatttta tggtccagga ttcagtcatt tcagtcaaac cgttgaagtt atgcataatg
     2221 aagaaggcga atccattgac cgagcaccaa atccaatgat gattttaact atgccagtcg
     2281 cacagccggt gaaacctgga gatatgatcc gaaaacgtaa ataaggaaaa agctgatata
     2341 tccctgtttt aagcatcttc gcatacaaaa ttcaagaaaa gtgttacact tgttttctgt
     2401 agaaaaagga tcgttaaata aaggttttga ttgatctctt caataaggag gaacaaaatg
     2461 caaatatatg attacatcgt aattggtggc ggctctggcg gtattgcttc agcaaacaga
     2521 gcagggatgc atggcgcaaa agttttgctg attgaagcga atgaattggg cggaacatgt
     2581 gtcaacgtcg gttgtgtacc taaaaaagtt atgtggcaag caagtacgat tttggaaaca
     2641 attgaacgag atgcctcaag ttacggtatc caagcagatt taaaaaatgt tgatttcgcc
     2701 ggtctagtag aaagacgaga aaaatatatt gattttctac acggtgctta tcaaagagga
     2761 ttagatagca atcatgtcga ggctataaaa ggttacgcta cttttatcga tagtcaaacg
     2821 atcgaagtaa acggtgaaac ctatcgtgct cctaaaatct tgatcgctac tggaggccgt
     2881 gcaagtaaaa tgaccattcc tggtggagaa tacgcaattg attcaaatgg attttttgaa
     2941 ttaacagagt tacctaagag agcaatcgtt ttaggtgctg gatacattgc ggctgaactt
     3001 tcaggtgtat ttcgtggttt aggtagtgaa gtcatgtggg catatcgaaa agaacgacca
     3061 cttagaacgt ttgataaaat gctttcggac aatttgatcc aaatgtacca agaagctgga
     3121 ataaaaactt acgcacatca tattgctaaa gaaattacta aaaacaatga cgaatatacg
     3181 gtgctatttg aaaatggtga aaccttaact ggtgattgtg tactttttgc cggtggacgt
     3241 gtaccgaata ccgaaaaatt aggtctggaa aatacaaatg tggaattgga caaaaaaggg
     3301 tttatcaaag tagataaata ccaaaataca acggatgaac atatttttgc tgttggtgat
     3361 gtgatcggta aattagatct gactcctgtt gcaatcgcag ctggtagacg tttatcagaa
     3421 cgtttattta atggaaagaa agattcttat cttgactata aacttgttcc gacagtcgtc
     3481 tttacgcatc caccaattgc aacaattggt ttgacagaag aagaagcatt ggaaaaatat
     3541 ggcgaaaatg aattgaaagt ttatcgttca cgctttaccc caatgtactt tgcattaaat
     3601 gattatcgcc aaaaatgcga aatgaaattg atctgtgtcg gaaaagaaga aagaatcgtc
     3661 ggcttacatg ctattggtat tggtgtcgat gagatgctgc aaggttttgc cgtggctatc
     3721 aagatgggtg caactaaaga agactttgac aatacagtag ccatccatcc aacaggagcg
     3781 gaagaattcg taacgatgcg ataatttaat attaacagtt ccaacagctt tttattgaac
     3841 aaaccatgta aaaccagaaa atcagtatac tgattttctg gttttttgtt ttttagttat
     3901 ttatgtaaga ttgcttcgat aattctcaaa acgaaataag tagggaagtt ggagattttg
     3961 attatcttcg taactgacga agagtaaatg aatccatttg gtatgcactc ttaagttcaa
     4021 aatattggta tcgatgttta aaagaaatag aaatgtatcg aagaacagat gtttaaaaaa
     4081 gtgtgacttt gtataaataa attagaagaa gcaaacgagg atgttaaaag aataccatat
     4141 agttaccaaa ccaagaaagt tctcgtcaag ttcgctggta cgaatcaaat caagtgaaaa
     4201 aagggactgt gaaaaaaatt gtgtcatagt tagtcacttt ttccgaacaa atagtgttca
     4261 atcagctgtc cttcggtatt agctcaaaaa acggcaaaat atgaggaact atttttccat
     4321 ttgtttctct aatactcagg acaaaacact gattttacaa cctctgataa acggtgttca
     4381 aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa tgtggaaagc cattttcaca
     4441 aaatttttct taatactcga aactaagcga ctttttcaca acctcctgat aaacggtgtt
     4501 caaaagcttg catctgcggt aataagccca accaaacgaa aaatataagg agctattttt
     4561 atttggctgt tcttattact tgatgcaggg cagttttttc acaacctctt catgctgtta
     4621 aatggcttgc ataatgattg ttagtaatag ccccaataca taactggcat tgaataagac
     4681 catatttttg atggaatagc tgaaactgat aggctggggt aacgtttgct tgaattgatc
     4741 caaatgcttc ttcacaattg gcaaggtcaa gaaggtgacc aagatcggcc attcatagat
     4801 accgctcaac aaccccatta aaatcacgaa atagcaagca tacatcaata gctggaataa
     4861 gactgttccc gcaggacgtc cgatataaaa aactagtgta tagcgatgat tttcaatatc
     4921 acggtcaaga tcccgcaaat tattagccaa catgatattg gcaatcgtaa acaccaaggg
     4981 taaggaagct aatacgattg ctgctatagc ccaaagattt cctaccaaag caaattgtcc
     5041 agatgaaaag ttgactgtca gatcatagac atctgaaccg aatgtattga cataaacgac
     5101 catggcaaaa attccaaggc ccatagtcac cccactgaaa atttccccta aaggcattcg
     5161 agataaagga atcggtccga atgtatagaa tactccaata aaacaacaga taaatcccat
     5221 caaaagtaaa agccaacccg tattgatcgt tagaataacg ccgataacaa aaacaaatgc
     5281 aatcatagct aaaatcatgt ttcgaatcaa tacaggagat agatttctgc ggccaatgac
     5341 attttcttca tacttatata cttcagaatg agccttttca taatccatgt aattattgat
     5401 agcggtagtc gctaaatcaa aaactagcat tccaataaaa aataaaaaag tgtttagcca
     5461 atgtatctca tgaaaaaaag cgatagagaa taatacccct accgcaaaag gaaatacact
     5521 agccaccttt gttcgtattt caactacttc taaaaagatt tttagtgtca tggttcgtac
     5581 tccttatttt agttcagaac ggtcacccat cgtgtaatgg gagtcctccg ataatttgaa
     5641 ggtgtctttg attggatcgg tcacatatac tttatcgtct tttgtcacga agatcgcttc
     5701 cgtcccgtta ttcaattcgc tttcgacgta ttccagtcct tttttcacac ccattgaaaa
     5761 gactgctgta gacaacccgt caccatcaat tgatttatct gttatgatcg tgacacttgc
     5821 gatatcattg tcgaacggat aacccgtttt tggatcaaac aaatgatggt attttttgcc
     5881 atctacttct aaataacgct cgtaaattcc agaggtaact aatgttttgt tccgttcttt
     5941 aatagaacct aaaacagatc cgcgagccat gttcggatct tggatgccga ctgtccagtc
     6001 ttggttttcg cctcttgggc tgtggcctaa gacatagaca ttaccgccca agtcaacgat
     6061 ggcagtggtc actccttgtt ttttcaagac tttcactact tcgtccgtaa tgtaaccttt
     6121 ggcgattgca cctaaatcga tgatcattcc tttttctttt agataaacag ttttttcttt
     6181 gtcatttagt tcgatcttat gataatccac taatttcaat gcctgatcga tttcttcttg
     6241 ggaaggtttt ctggcgtcat caaaaccgat tcgccataat tgtgtgattg caccaatcgc
     6301 catatcgaat ccgccttgag aatcttggct atattcgtag gcccgcttga ctaacgtgta
     6361 gacgtcatca gaaactttta ctggtttgat cccagcttgc tcattcactt catcgatctc
     6421 agaacctttt tgattgatcg tgattttatc accaagttct tttactcgat cgaaggcagg
     6481 tttcaatgct gattctttgc cttcatcata cacacggata cgaacgtaag tccctaacaa
     6541 gaactgttca tcagaatatg gatctttcaa tatcttggta gctgctgaac tttcagtggt
     6601 tgttttattt gctgagttgc tgcagccagt tagcaccata aaagagatgc ccagtgtgat
     6661 taaggaaaat agtagttttt tccgcatctt tttcctctct tcattttgaa aggtgagacg
     6721 actgcctaat ctggctatcg tcccaccctc caacttttat tagtatgatt atgtgattat
     6781 ttttctgtta cgatattatc tacttcgatt gtttgagtgt cgcctttttc agctgcattc
     6841 actaattgtt gtgcgtacat gatgaatgaa tgtgagctgt gtgtagcacc tgtaacaact
     6901 tctacattag caggtgagcc atcttcttcg cccattgctt tgacgaattc gtcgttcaga
     6961 gtagggatgt aagtttttgg ttctgtaccg ctttttgctt tcatattttc attatattct
     7021 gtatcatctt gttttgattt accgtctttg tcgatataat caaagtttga ttcagttact
     7081 ttgccgccag caactgtcat ttcgaattgc acacggtagc cgttagagta gttcttttct
     7141 tcaagcttgt aagtgccgtc tttcaaatca gcaccattgt cgatttcgat tgtatctgtg
     7201 ttaccagctt gagcagcttg gatcaattgt tgtgcgtagt tttggaagct ttcagatgag
     7261 tgagtagctc cagttacgac ttccacaccg cttgcgcttt gtgcttttaa gaattggtca
     7321 ttcaactcag gaatgtattt ttctgggttt gtgccagatt ttgcttccat gtttttgttg
     7381 tattctgcat cttttgtttt tgattcgccg ttttcattga cgttatcata ttttgattca
     7441 gtgattttgc cgtcttttac aaccatttca aagactgcac ggtaaccatt tgaataattt
     7501 ttttcttcta atttatatgt gccgtcttgc aaatctccgc cagctacaac ttttgcagat
     7561 gattctgttg tgctttttgc agtcgaagat tgtgctgtag atgattcaga agaagagttt
     7621 gagcttccgt tgtcatctgc gccgcaagcg cctaacacta gagctgaaaa tgctaatacg
     7681 gcaaatccag ttacaaaacg ttttttcttc attttaatac cccaccattc catttctagt
     7741 ccacaatttt tctttgtgat tcattataca ttctaatctt tttagcctat aagt
//
LOCUS       DAYYME010000122         7722 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000122 DAYYME010000000
VERSION     DAYYME010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.123"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..107
                     /locus_tag="ACHBFW_002319"
     CDS             <1..107
                     /locus_tag="ACHBFW_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFM9318588.1"
                     /translation="NESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(176..904)
                     /locus_tag="ACHBFW_002320"
     CDS             complement(176..904)
                     /locus_tag="ACHBFW_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM9318589.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(917..2197)
                     /locus_tag="ACHBFW_002321"
     CDS             complement(917..2197)
                     /locus_tag="ACHBFW_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM9318590.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2338..2940)
                     /locus_tag="ACHBFW_002322"
     CDS             complement(2338..2940)
                     /locus_tag="ACHBFW_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM9318591.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3185..3391
                     /locus_tag="ACHBFW_002323"
     CDS             3185..3391
                     /locus_tag="ACHBFW_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318592.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3703..4422
                     /locus_tag="ACHBFW_002324"
     CDS             3703..4422
                     /locus_tag="ACHBFW_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM9318593.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4503..5186)
                     /locus_tag="ACHBFW_002325"
     CDS             complement(4503..5186)
                     /locus_tag="ACHBFW_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFM9318594.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5405..7591
                     /locus_tag="ACHBFW_002326"
     CDS             5405..7591
                     /locus_tag="ACHBFW_002326"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFM9318595.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2770 a         1301 c         1456 g         2195 t
ORIGIN      
        1 gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag
       61 atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaaga
      121 accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt tctttttatg
      181 aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt gccaacgtga
      241 cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca taggatttat
      301 gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca tagaattgta
      361 attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat
      421 attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt ttgaagatat
      481 ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata tgctcgacat
      541 tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg atgttgcttt
      601 ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt cctaaaggat
      661 ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct tctagttcga
      721 tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg agaagttctt
      781 ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg attctatttt
      841 tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata taaatgggaa
      901 tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa cgctggtttt
      961 caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc aagataaaca
     1021 agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt ttcaactggt
     1081 ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa tgattcttct
     1141 tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca atcgcattgt
     1201 ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg
     1261 gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg agttttaatg
     1321 acagtaaact acggatctca ggaactttca tattatccaa catacttaga ttggccggaa
     1381 aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac cgtaatcctt
     1441 ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg taatcatgtt
     1501 tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc tgttgcatcc
     1561 tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc caataataaa
     1621 ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc aaagctaaga
     1681 catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat gtaaataata
     1741 tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa gtctctaacg
     1801 atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca atgtgaaaat
     1861 actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc
     1921 ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg taaaccaatt
     1981 gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata gcaattagag
     2041 cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa atcagaatga
     2101 ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata caaattgtgt
     2161 aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc cattgatttt
     2221 taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat tttgaattca
     2281 gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt taattgttta
     2341 atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg taataggaag
     2401 caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt aagaagctga
     2461 gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct ttaataatgg
     2521 gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat agcgaagcag
     2581 taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag tcaattgcag
     2641 atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca gacaaacgaa
     2701 ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag aaagaaaggt
     2761 gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga ccgctagttt
     2821 tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca ccatatcttc
     2881 ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt tatctttcat
     2941 ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc
     3001 ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct tcgttttcca
     3061 agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat gacaagtttg
     3121 tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg atggagggat
     3181 tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta aatctgattt
     3241 atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg tgatgatacg
     3301 tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact ggcagttagt
     3361 tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag agcataaaga
     3421 ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa acagcttttc
     3481 atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg tttatttgaa
     3541 aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg agttactttt
     3601 ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt tatttctata
     3661 aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat aattgtgatc
     3721 attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct tgcccaagat
     3781 ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc gctattttat
     3841 tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa acgctttttg
     3901 gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat ctacgaagat
     3961 agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga agtaaagcag
     4021 tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc acaaaagaaa
     4081 ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa
     4141 agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta ctataaagaa
     4201 ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa aatagtgatc
     4261 gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa
     4321 atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat ccgctacact
     4381 acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa ctaagcaaat
     4441 tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt gattcattga
     4501 gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt ctgagtgagg
     4561 gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga
     4621 cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag atagtggaaa
     4681 aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt cgttgctacc
     4741 ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat tttatcaaaa
     4801 gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc gggatgctca
     4861 tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag aacaaacaag
     4921 cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt tagaagaatc
     4981 cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca ttctccgtca
     5041 aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa agcaccatat
     5101 tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag tcctattgta
     5161 cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata tttttgtctt
     5221 ccttatctta gcataaacga ttgaaaaaaa aggaaaaatt ttcagaaaac taaaatcata
     5281 tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat agacaacgaa
     5341 actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga aggaaatcaa
     5401 tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct gggagaacaa
     5461 gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta cctatgaagc
     5521 ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag aaagtgaagg
     5581 aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca atcaagaaca
     5641 aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag gagctgggag
     5701 cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa aacaagtaga
     5761 cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac taaaaaaacg
     5821 agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc atagtatctt
     5881 ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc aaggaataca
     5941 aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac agctgatacg
     6001 taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt ctcggttaga
     6061 ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta aagaaaaata
     6121 tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg gattgctcac
     6181 ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg tactccaaaa
     6241 tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa aatatgatta
     6301 tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg tgaaacggat
     6361 gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga gtatctatgg
     6421 attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc ctaccaaaac
     6481 tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag caaatagatt
     6541 gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac aaaaagaagc
     6601 aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt ggctaatcaa
     6661 acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca tattataccg
     6721 atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata ttccttttgt
     6781 actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc aacatttcta
     6841 tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc aggcctacca
     6901 gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat gcgatcaata
     6961 cttttctcct aaatatcaaa atatagccgt tgcagattat attttgtcga taagccccaa
     7021 tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta aaacagacca
     7081 gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta tagctaagga
     7141 aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg ctactttcag
     7201 aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa aaacccgctt
     7261 gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc acaaaagtaa
     7321 aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt tgccttataa
     7381 cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac cagtaacgac
     7441 gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa atcgtctgta
     7501 tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat ttatcaaaga
     7561 aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca gaagtggtca
     7621 gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt gaaatttcag
     7681 cttattttcg aagaagctat ttctggaaca cagtttattc gg
//
LOCUS       DAYYME010000123         7637 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000123 DAYYME010000000
VERSION     DAYYME010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.124"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(279..2237)
                     /gene="nagE"
                     /locus_tag="ACHBFW_002327"
     CDS             complement(279..2237)
                     /gene="nagE"
                     /locus_tag="ACHBFW_002327"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM9318596.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2234..3106)
                     /locus_tag="ACHBFW_002328"
     CDS             complement(2234..3106)
                     /locus_tag="ACHBFW_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9318597.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3448..4320)
                     /locus_tag="ACHBFW_002329"
     CDS             complement(3448..4320)
                     /locus_tag="ACHBFW_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9318598.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4448..5017
                     /locus_tag="ACHBFW_002330"
     CDS             4448..5017
                     /locus_tag="ACHBFW_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM9318599.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5084..5821
                     /locus_tag="ACHBFW_002331"
     CDS             5084..5821
                     /locus_tag="ACHBFW_002331"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9318600.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            6052..7320
                     /locus_tag="ACHBFW_002332"
     CDS             6052..7320
                     /locus_tag="ACHBFW_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM9318601.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
BASE COUNT         2384 a         1493 c         1520 g         2240 t
ORIGIN      
        1 tgtgaaaatg gctttccaca tttttgcata aatttgactt cataccgata aactggtttt
       61 tgaacaccgt ttatcagagg ttgtaaaaaa gctgttctgc atcaagtaat aagaagagtc
      121 aaataaaaaa tagctcttca tatgttacgt ttggctggac ttattaccgc ggagttggca
      181 ggagaacgcc gtttttattc ggaaaaagga gctgagacaa gatttttgtc tcagctcctg
      241 tttgtttatc gataaaaaag tctggggaag acgatggctc aggctttgtt ggaaacagag
      301 ccgattttat catttgcttt tacatcacgt gcgggttcga tttctaagct ttggactttg
      361 tcgccatttg tgaatacaac gaccatatcc gtgtttttgc ctgcagattg aatagcggca
      421 agatcaacta aagcaatcaa ttgacctctt gcgacttttt gaccttcttt tacatataaa
      481 gtgaatggtt cgccttttaa gtcaacagtg ttgatgccca tatgaagcaa gacttcgata
      541 ccagcatctg tttggattcc gacagcatgt ttcgttggga aaatgtttgt gatcgtacca
      601 gcgattggag agaagatttc tccattttct ggtaatacag catagccgtc acccatcatt
      661 ttttccgcga atacatcatc tgaaacttca gtgattggaa tcagtttccc atttgcgata
      721 gcgtaaatat cttgttcgat cgttccaccg gcaaatgttt ctgttttaac cacggtgttt
      781 gtttgtgcag gtcgtgttgc agcaccacca tgtagttttg ccatctcatc tgcaacgaat
      841 tgtacttctg tacctacgat gacttgcgca ttttttccgt cgatgatctt cataccaggt
      901 acgccagtgg ctttgatttt attttgatcg atcaggcctg tatctttgac ttgcaaacga
      961 aggcgtgtag tacagttatc gatcgaagtt acattgtcgg ctccgcctaa tgcagcgtag
     1021 attttgcttg cttgtgccgc atatttattg tctgaattag tcgttgcagt atcgtcatct
     1081 gaaagatctg cttcctcgcg gccaggagtc atcagatgga attttttgat agcaaagtta
     1141 aagccaaagt agtaaatcac tgctgttact aatccttgga cgatcaacat caatggctga
     1201 ttcgcaatcg gatttttcaa agataggaag taatcgacaa atccggcact aaaggcaaat
     1261 cctgctgtcc aatggaagaa tgcactcact gctaaagaaa tcccagtgaa aattgcatgc
     1321 aagacatata atggccaagc aacgaacata aatgagaatt caagtggttc tgttacacca
     1381 gtaaagaatg aagcaaaagc tgctgctaac atcaaggaag ctgtttttgc tttgttttct
     1441 ggacgtgcat ttctgtaaat agcatatgct cctgctggaa gcccgaacat catgattggg
     1501 aaaaatccag cttgatacat tcctgttacg ccttttgttc cttcagaagc caaaaattta
     1561 ccaatatcat tgattcctgc tacgtcaaac cagaacacgg agttcattgc ttgatgcaat
     1621 cctgtaggga tcagtaaacg gttgaagaag ccataaagac ctgcaccacc ccagcctaaa
     1681 ctcaaaatga atttagcaaa agaaactaaa gcagagtaaa ccggaggcca tacgagcagt
     1741 agcactgcac tgatcagcaa catgacaaag gcagccaaga ttggcactag acgcttgccg
     1801 ctaaagaaag ataaagccat cggtaatttt gcttcgtgga aacgattata aagagctgcc
     1861 gcaatcaatc cggcaataat accaataaat acgttactga tctgagcaaa agatggatca
     1921 actgattctg gtttgatatt caataaagta gctactgaat cagtttttaa aacatttact
     1981 ggaacttcaa atgcgaccaa acctgccaag gcagcagcac ctgatttgtc cactgacatc
     2041 cctagtgcta acccaacggc aaacagcaaa ggtaattgac ctaaaactgc gttaccgcca
     2101 gcttgtaaaa aatgagcgac aatgtcgtta ctataagatg cccaccagtt tccgatccct
     2161 actagtaaag cagcagcagg taagaccgca actggaagtt gaagggaacg tcccattcgt
     2221 tgtagatatg ctttcaattt ttttcctcct catgattgtt aacatttgtt tgtacgattg
     2281 cgcttcttaa acgctcgata tggatggcca agtaacccag ttcttctttc gtgatcgcga
     2341 gctggtattc tttggtcagc aacacacgga tttttttaga aatcttatag ctttctgggt
     2401 attttcgttt gaccatttct acgatttccg tgtctaaaac agcatattgt tgcgcatggg
     2461 ttcgttgtag aagcagtcgt aaatgattca ccagtcttga ataattcagt gaaaatggtt
     2521 ctgaatggat atctgtttcc aattctgatt ccactaaatg gatgatgtca gatacaatcg
     2581 tcacttcacg gatactgcga tggttattgt tccgaccgct tcgtccgctg tgaatatgga
     2641 ttgcgatata tcctgcttcg tcataagtat atggaatccc aagctctttt gtcagatact
     2701 ctactgccca ttgagcaata ctgaattcat cactgtaaag cacttcgatt tctcttaaaa
     2761 gtttgttgcg gatgacgata ccgttattaa tgttctccgc agaaaaagca atgtggtcgg
     2821 tcaaagcgat caatacatgt tcattcaact tttccatcaa aacagtttcc gcatgatcaa
     2881 tgatcgtttc tgctgcaaat aaaaagcgtt catcaatctg attaagtaaa ttttgcaact
     2941 tgatttgacc ttctggttcc atgacaaata tccgttcgac atcacgatca aaaatcaaat
     3001 catttctttt tctgtcaaat ccaatccctt ttccaatagc tactttttcc tgtccaccgt
     3061 catctaccaa tactgcattt tgattaaaga tctttttgat tttcatgcgc gcaccctttt
     3121 tgaagatttg gttttcacgt ttgctagacc agttgattgc ggccaaacaa aaatagacac
     3181 gaaaaacctc ctagggaaac catgttcctc agagattttt catgcctagt atgatctagt
     3241 cacatgaaac gaattattgt taacgtaatc aattttatat agacagttat gttttgtcaa
     3301 ttcttttttt ataattatag taatttactg gaattatcag aaataaaagg aaggaacaaa
     3361 ttaagaaact tatgttattt aatcattgga tcttttgctt aacttattcg ttgttaaaaa
     3421 gctgaaagcg aaggaaaaaa actctactta actgtatttc tccgttagga catctaaaaa
     3481 tttacgagca gcaggagaaa gaggctggtc ttttttccaa atgagattca gtgaagcttg
     3541 taatggcgga tcgagtggga tgaatacttt ttccgtccca taagtattga tgatcccatc
     3601 tagagctaat gcaccaaaag tcccgttatc gaccatcaaa gaagcattgt acaataagtt
     3661 atatgttccg acgatcgata actgatctat gctgaaacct agccattcag ctagttgact
     3721 gtccactgac gtttgccggg aaatgatcaa aggaacagtt ttcagttcct gcggatggat
     3781 gactgtttgc tttgctaaag gatgattccg atgtatcaaa agtccccaac gatcttgggc
     3841 tggcagtcga aatgactggt atttttgttt gtttgtcggt tcgattacga gtccaaaatc
     3901 aagtaatccg ctatccagtt tgtctatgat atcatctgca ttcccgctat ataaatgaaa
     3961 ctggatatca ggatgcgcct tgatcaacga atgaaggcta tcagaaatga aagagattgc
     4021 tttagtttcg cctccgccaa tgtagatttc tccagagatg attgcgtcat ttgttaaatt
     4081 gttcgccgtt ttttctacca acgacaagat ttcttttcct tttgaataaa gatatcgacc
     4141 agaatccgtc aactgtatct ctcgggagcc tcgagtgaaa agtgtcacac caagttcttc
     4201 ttctaaatct ttcagttgtt ttgagagagt aggttgcgtg atatgcagag attttgctgc
     4261 tcggctgata tttttttctc tagctactgc taaaaaatac gtcagtatac gaatatccat
     4321 tttttccctc ctgtttttaa aaaagcatac cactaagtat agttaaaagg aatagttaag
     4381 tatgttttat aagtattaga catcataagc cgtccgttat aaagtaagga taaaaggagg
     4441 gagcaaaatg acaagcctca tcgaaaaaca aatcgtcaat aaagagattc gagtaacgga
     4501 tccgctcttt gaagtgatcc atcagatcca agcggaaaac gaagagcctt tagcagcgct
     4561 gaatactggg taccatgaac cagcagatat ccgcaaacgg ctagaaaata tcattagtga
     4621 caaagtagat gatacggtga ctgttctgct gcctttttat acagatttcg gcaaacatat
     4681 tagtataggg aaaaatgtct ttatcaatcg tcaagtgatg ttcgttgact taggtggcat
     4741 ctgtttagaa gattcagtat tgattggtcc tcgtgtcaac ctcatcacgg tgaaccatct
     4801 aaccgatcca aaagaacgaa gaggcttgtc agtaaagcca atccacatca agaaaaatgc
     4861 ttggatcgga gcaggagcaa ctattctgcc aggcgtaacg attggcgaga atgccattgt
     4921 tgcagcgaat gcaactgtta cgaaagatgt cccagacaat accatcgttg caggaatccc
     4981 tgccaaaatc gtcaaacctg tcgaacggat tatttgaaca gtaagatgaa ataagagata
     5041 gtgagtataa gcaagagatt tttcaaaaca ggaggagaat agaatgaatg atttatcaga
     5101 aaaagtgatt gtcatcatgg gcgcttcaag cggaatcggt gaagcaacaa cgaagctatt
     5161 agcagaaaaa ggggctaaac tagttatagc tgcacgccga gaagatcgcc taaaagcaat
     5221 aaaagaatct ttaccagaag ctgagctata tattcaaaca gcagatgtaa gagattttgc
     5281 acaagtccaa gcagttatcg atttagcgat ggaaaagttc ggtcgaatcg atgtattgta
     5341 caataatgca ggaatcatgc caaccgctcc attagttgaa ggtcaccgtg atgaatggca
     5401 aaatatgctt gatatcaaca tcatgggtgt gttaaatgga atttccgccg tacttccaat
     5461 catggaaaaa caaaaatctg ggcatatcat ttcaactgat tctgtggcgg gacatgttgt
     5521 ttatcctgat tccgccgttt attgcggaac aaaatttgct gtccgtgcaa tcatggaagg
     5581 attacgtcaa gagcaacggc aaaataacat caaatctacg atcatctctc caggagctgt
     5641 tcaaactgaa ttatatcaaa ccatcagcga caaaaaggct gctcttgaac ttcatgaagc
     5701 gcaaaaagaa tggggattaa catctgaaga tatagcttct gctgtagcat ttgcgataga
     5761 aacgccagac cggatgtctg tcagcaacat gattatccgt ccgacgacac aggaagtata
     5821 atccataacg gataatatcc agagagaatt acttgttaaa tagttattca aacgaccatc
     5881 aatttaaggc gtgaatggag aagaatattc ttcatttacg tctttttatg cagaaaaagc
     5941 aaatttatta aaaagaaaca aatgttattt aaatatggac attgacttgc ttttactttt
     6001 ttagttacac tgttatttag gaaaagtttc ataattcaaa ggagaaaaaa gatgaaagca
     6061 aagaatttat tatttagcgt tattctttta ggaaatctag cagttgccaa cacaacagtg
     6121 ctagctgaag aatcgacaga aactgtcgca actcagacat cgagttcttc agaaacagga
     6181 attgaaaacg atacagaaag tactttgcag tcagctactg tctcagaaaa tacaatttca
     6241 accgaagaac caacaactga aacaacagaa caaacacaaa ccgaagacag tacatcagca
     6301 gaagctcaga acaatccaga gaatgcagaa gaaatcacga accaattgac agatgggcaa
     6361 acattgacac atacccatca agcagatttg tctgaattcg aaaactatac tggctatgct
     6421 ttttatgctc gctcatctgc taattctcaa caagcgttca ttgatagtat tgcctcaact
     6481 gcacaaagtt tggcaagtgc caatgactta tatgcatcag tcatgattgc tcaagccatc
     6541 gttgaaagtg gctgggggaa cagtactttg gcttctgccc ctaattacaa tctatttggg
     6601 attaaaggaa gctataatgg ccaatctgtt acgatgccta cttcggaata tgtgaatggg
     6661 caatggatta cggtgaatgc cgcttttaga aaatatcctt cgtacaaaga atcattgcaa
     6721 gataacgtaa cggtattgaa gacgacttct tttcagccag gcgtttacta ctattccggt
     6781 gcatggaaaa gcaatacgaa ctcttacaaa gatgctacgg cttggctaac aggtcgttat
     6841 gccactgcgc ctaattatgg ttctacattg aacaatgtaa tcgaaaccta taatctgaca
     6901 cagtatgata cagcaccaac ttcaacttct ccgatgtatc gtctgtataa ccgtcataca
     6961 ggagaacatt tatatacatt gaatgccgga gaaaaaaatt atctgccaac tgtaggctgg
     7021 gaatatgaag ggattgcttg gcaagcacca aacagcggac aacctgtcta ccgtttatac
     7081 aatccttaca gtggagacca tcattacaca atggcacaat ctgagatcaa ctctttaaca
     7141 aaaatcggtt ggcgttatga aggtctaagt ttttattctg gtggttccaa gccaatttat
     7201 cgactattta atccaaatga aaaaactggt acccatcact ataccttgag tgctaaggaa
     7261 agagattggc taacaccgat gggctggaga tatgaaggaa taggatttta tggttattaa
     7321 aaagaggtag tgaaaaagct gccctgcatc aagtagtaag aacagccaaa caaaaacaga
     7381 ttttcatgtt ttttgtttgg ttgaacttat taccgcagat gcaagttttt gaacaccgtt
     7441 tactcaaaaa aggttgtgac aacatcttgt cataactttt ttattactgt ttaagcatac
     7501 tgatccaatt tctaatattc gacgggattc ttgcaaaagg cgatttcgga aatttttggc
     7561 ggtgtaaaat tatctactgc agaagagcta tcttctatag tttgataaga atcaataatc
     7621 ttggttttag cgttgtg
//
LOCUS       DAYYME010000124         7621 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000124 DAYYME010000000
VERSION     DAYYME010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.125"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(228..953)
                     /locus_tag="ACHBFW_002333"
     CDS             complement(228..953)
                     /locus_tag="ACHBFW_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318602.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1107..1433)
                     /locus_tag="ACHBFW_002334"
     CDS             complement(1107..1433)
                     /locus_tag="ACHBFW_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318603.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(1470..1835)
                     /locus_tag="ACHBFW_002335"
     CDS             complement(1470..1835)
                     /locus_tag="ACHBFW_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFM9318604.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(1872..2087)
                     /locus_tag="ACHBFW_002336"
     CDS             complement(1872..2087)
                     /locus_tag="ACHBFW_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9318605.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            2216..2551
                     /locus_tag="ACHBFW_002337"
     CDS             2216..2551
                     /locus_tag="ACHBFW_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9318606.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(2627..4624)
                     /locus_tag="ACHBFW_002338"
     CDS             complement(2627..4624)
                     /locus_tag="ACHBFW_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318607.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(4722..4997)
                     /locus_tag="ACHBFW_002339"
     CDS             complement(4722..4997)
                     /locus_tag="ACHBFW_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9318608.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            5229..5963
                     /locus_tag="ACHBFW_002340"
     CDS             5229..5963
                     /locus_tag="ACHBFW_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9318609.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            6002..7144
                     /locus_tag="ACHBFW_002341"
     CDS             6002..7144
                     /locus_tag="ACHBFW_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9318610.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         2573 a         1387 c         1095 g         2566 t
ORIGIN      
        1 tcttaaaaat tttttcacta aaatcactac ctgaaacagt ctaaaacact tatatatcaa
       61 cgtttaatga aactatctcc gtttcatttc agatacaaat aaatatagtc gggctccaaa
      121 tccgaaaaaa aataccccca gtagcttatc ctaagctact ggagatgtat aagtacggtc
      181 atataacaac ggggtagggt aaataaatga aaaagatgga agaatcatta ttcaataact
      241 tttttaaata cttcgttgta atcagttacc tgtccgttct taaataccac agaataatct
      301 tttttcccat tttcaagtaa ccgaagtgga atatctttac caactttctc tgtcactgca
      361 ttactgatac ctgttagatt agctaataaa tctgataaag attgagcatc tgtagaaatc
      421 tctgtatcag tatttatcgt gattccttta tcttcattta caagtttcca agtaggtggt
      481 atagctgtgt atgtttctac aaatgaatta agtttttcat taagtgactt ctcttttata
      541 tttccatcta aaaactctgt atatatggct tgagctatac taaaacaaag ctggccttgt
      601 tctaataatt ttacatcatt agttagtaaa gctgtgttca cgttatcaat cgcttcaaga
      661 agagtatctt tggtgtccct aacagcttta ggtgtgcctt ctggaatttc cctacttgat
      721 acctcgtcca ttttatcttc cataaacccc ttaaaatcat atctttctcc caaatccata
      781 gaagtttttg agaaagcttt catttctgag tctatttctt caataatatt taacatgtct
      841 gatacatatt gtttaggctg attcgttttt aatctatatg tggtaaatat ctcaattcct
      901 ccaattatca ataaaattgc caaacaaatc cataataatt ttttcttttt catttttcct
      961 cctatacact gctgttataa cagtgactaa tgtaaagatt ttcccagcaa tcatcagact
     1021 attatttaca gggaatcaaa aaaactcctt agtaagctta cacctttagg agttcctgtt
     1081 tgaacggtat gcataataca tttaaatcaa tttaacatta acacctcagt atatggtatc
     1141 tctacatata cacggtcttt ttttggatag tactcgacat ttatattgat agcacttcca
     1201 ttgtctaacg tagctttagc ccggtacgtg ttatcaccat tatcatgagt gatcgtcaca
     1261 tcatcacatg tccgatttaa gttatactgt tcttgtaaaa tctgagttac caatggtaca
     1321 gatgacttct ctagtccaga tgaacccgtc attcttgaaa aaattatacc tataatagcc
     1381 aatacaacga tacctataat gacaaacttc ttattcatat ttactttatt caacttttta
     1441 tctcctatct ccaaaatgat tttctctcat cagtaactca ttgacatccc caatagtaca
     1501 ccaaagatgg ctccagctac ccctgctatc atcaaaatcg tacccatttg ttcatctttc
     1561 tctcgatata agtaacccat aataacagca acagcaccaa ataccacagg aataaatgct
     1621 aatgatacta ccatagaaat ccaaccaata atcaaataca gcttccctgt ctcttgtcca
     1681 tgtacgattt gttccttcat ttcattaatt gtctgctcag tacttttctt tactgtgtct
     1741 gttccggttg tctcatctgt ttcttgtaat gctccgcaaa actggcaata cttactttca
     1801 tttgttattt ctttaccaca ttttctacaa tacatgtctt ctcctcctaa aatcattcaa
     1861 ttgcttatta gctaactgtt tactttatta aatacatcag aaaacagctc atttattggt
     1921 acacttaata tctcagaaag cattttcatt tgatagggtt taggaatcgt tctccctgtt
     1981 tgccacgaac ttacacattg ctgtgtaaca ttcatttttt tggctaactc agtttgggaa
     2041 agtccttttt cttttctgta tcggctgaga ttacttactt ttttcatgat tctcccctcc
     2101 tgttttatta tatacaacaa tatgatgtat ttcaactcat aaaatatata aatacttcaa
     2161 atgtcattac aaatacaaca acttgttgta ctctactgag aaggggggtg tattaatgaa
     2221 acagaagcaa tatgcaaagg tcattagaga aaagcgaaaa gcactcgggt ggacacaaaa
     2281 agaattagct aaaaaaatct tctctacaca acaggcagta gcaagatggg aaaattctgt
     2341 aacagaacct aacctcgata gcttaacagc tctttctaga gccctaggaa cccctgtaag
     2401 ccattttcta gacaatgttg acatagatta tgaggaagag tttttggctc tctatcgctc
     2461 tcttagtact gaagatgcaa ttcgaacaat tgactatatg aagctattaa aaaggcaaga
     2521 aaatgaacgc aatcagctat tgaaagattg aaaaataaat aacccaccga actttaacgg
     2581 tgggtgtatt gagatgaaat aagattaaga gctatggata acctgtctag tttaatataa
     2641 ttgggtctct tctattcctt actctccgtc catcatctct tgttacactt gtaaattgat
     2701 aaataggaac taattcaact cctgataata tcaattcttt tattacttca tctatccctg
     2761 cccctttcgc tagatatact aaaaaactac tgcttgcttt cacattgtat agtttcttaa
     2821 tatccttaga agctcgttca agagtgaatc cttgtttaac aaagtagcct ctgttttcta
     2881 ttaaagcatt tttcacgctg ttatccctga catttcgtag gcgttcacaa acagtgttaa
     2941 taagttcttt ttctgtcatt agatatgtcc cttgccacga taagccctgt aaaacttcat
     3001 gtgcttcatc taaaatcaat tgtttttcct tgctaagttc tgatcgggaa ttttgaggaa
     3061 atactctttt agcctctttt tcgttaaaac atatcttaac ccattcataa gaaaaatatt
     3121 gatatgtaaa atgaaatttt tccagaagtg gaaggatatc ttcattcaat ttttgaatca
     3181 cagaagttgc attaaaaacg tctaatcgat agacattgct tctcttatgt ttaaagtttc
     3241 ctaacgcttt ctcttccttt aacctattta ggtattcgag catcttcaaa ggaacatctt
     3301 catcatctaa tttttttatc aatcctataa aagtcataag tgatagtaac ttattacatt
     3361 tatctatgtc ataagtttta ccatagtaag aactcatttt tttagctaat tgttcccccg
     3421 acaaaataca agtcacactt tccttactat catcaaaaaa attaacatca gaaagcaaga
     3481 catcatagac acgtcgcatt tcctctagat aattccttat tgtcctgaaa agtttgtctt
     3541 tcattgattc aagagtgtca aaaagtactt ttgattcctc tttcaatttt cgaaaatgtg
     3601 taggatacaa gtctaggttc tctaatagcc aattaaatgt ttctacccga ctgtctttct
     3661 ttaaaattag catcacaact ccaataaaat tttcactgat atcacaatta ctggaatgac
     3721 aataaaaaat ggaatgccca gttttactgg taaaaatact agcagaggga ttatgatcgt
     3781 tgtgaaacag acatcttaag tttttacttg acttaagtaa tttattcata tcaatggtta
     3841 acaatctttc ataaatctca ttaaaagaca tgtcggagga atcaaacagg cagtctttaa
     3901 accactgttt catttcttct ttgtcgtcat tttttatcaa cttaacaaaa tcggtttcat
     3961 tgactagttc atactctctc cctgaatcac tttgacaatt tttagaacat gtcatggtaa
     4021 aatcaatcgg aagctcagta atatctaaca tgttgtcttc attaattatt atcactgcat
     4081 ctgacttata tcctccaaaa aacattctat tcgaagcatt tacattgtca tcacacccgg
     4141 ggatgttctt tttcaagtat tcgtaaacag caaccatttt accatagtca gtaattgctt
     4201 cttttaatag aaagacaatc ttgtattttt ctagattttc agagtaccga atactttttt
     4261 gtatcgcaaa cgcagaatta tgaactaaat tgatagataa aaccttttct atgcttaagt
     4321 atctactatc atctttactc aaatctacta ccttcccctt ctcatcttta acagtgttat
     4381 cgacatccag aataattact ctggtttcca caaaactctc cttgtcattg cctttattct
     4441 ttgtcttgct tggaataaat gattgtgatg aatttagtat ctctacaaac tcatctcttg
     4501 taacttctct atagttatct aaacacctat ctcttacatc agttttttct ttttttgtaa
     4561 ctggcttctc taataatttt tctttatcta cataaattgg aatattaatc attgatgttt
     4621 ccatttgtgt ttcctcctta taaaattctg ttaaagacta aaacatacag ataataaaaa
     4681 caaactcttt attactatat gttttagtct tatttgttta ctcaaccttt ataccgttcc
     4741 atgaaactat caatatcaga ttttttaaat ctaatgaccg tatcaatttt tataacaggt
     4801 aacccgtatt gagaaacata ttttctaatc gttcctactg atacacctag atattcactt
     4861 gcctgctgat agtccatata ctctagagtg actccagaaa taatgtcttc tagcataggc
     4921 tttagcactg taattatctc tttcacttgt tgttcaactt gtatagaaac aatgctatca
     4981 atgatttgta gagacatatt tccccctcct ttcgtgattg cttgaacaaa ttttctaaga
     5041 attatcttcg tattttttgc tcaaaataaa agtattttgt tattaaccga ttaatcaaac
     5101 aaaaaatgat taccagttca tcgacataca cataatacct cgataaaaaa ggggtgtcaa
     5161 tagttttgta aaatttattt acaacacccc ttttttgagg tatactaatt ttaggaggtg
     5221 tggtcaatat gataaaacgt ccaccaataa attatttaga acgaaaaaag atactcggaa
     5281 caaaaataaa agctataaga aagagtaaaa aattaacaca accagcattt ggtcttatga
     5341 ttaacaacgg tcaattaatt gacaaaaaga ctatctacga atgggagaag ggaacctatt
     5401 taccaatacc agagcgatta tcccgaattg ctgatttggg aaatatgagt atagaggagt
     5461 tagtttgtgg caatgtagaa gaatatattt tgggaattat tctttataga gatagcattg
     5521 tactagatgg tataacattt ccagataaaa acctgttcca gcatcttcgt caacagttcc
     5581 caccagtaca ctctaattta gatacatggc tggatagata ttccaaatta gaaccagaga
     5641 tgcaagaatt tattgctaac aagacttgta ataaagttaa aaatgaaaaa attagtcttt
     5701 tcaatatctt aaaaatagaa gaattattca tcaatgctat tgtagaagaa tttgacaata
     5761 acattctttt cctcactagc agtatagaag aactactaga aagaatggta gatgaatggc
     5821 tacctattca attgaaagac atgtcttatc ccgaagaagc tgtgagagaa attactgata
     5881 atataaacaa gctagaacaa acgatttcat caattgggaa aaaatacact aagaaaaaaa
     5941 tgaagggagg tgataccatc tagtggtaag tactccttgt aaactaagag gagatgattt
     6001 catggcaaaa atatcaaagt acaaaaagaa aaacggtcaa gtggcgtgga tgtataaagg
     6061 acacattgcc accgatccac gaactggtga aaaaataaat actacgagaa gaggttttag
     6121 tagcaaacaa gaagctcaaa aagattatga cgagtacaga catcaaatat tatacggtgt
     6181 taaaaagaaa gcacctgata tgacatttga agacctgtat aatgaatgga taactcatca
     6241 gcgtaccagt gttaaagctt caacaatcgc aatctcagta agatacgcaa ataatcaaat
     6301 acttccagca tttggtaagc ttaaactatc gaatatctct gtcccatatt gtcaaaaagt
     6361 ggtagatgaa tggcacagta aatatgaaag ttacgattat atgagaaaac agactgcaca
     6421 aattttacgc tacggggttg caatgcaata tattgacaat aatcctatgg aaaaaacact
     6481 tcttccacga aaaaaagagt atgagaaaaa tcgtaaattt tactcaaaag aggagcttaa
     6541 taatttactg gatgctttta aagattttgg taacatgaaa caatacgcct tcttcagatt
     6601 attggcatac acaggtatga gaaagagtga agtactcgct ttacagtgga aagacataga
     6661 cacgtttaat aaagaactac atgtcaataa aactcttgct gtagatgaat ttggtaaagt
     6721 tataattcaa tctccgaaaa caagagcttc tagacgagtt atctctttag atactgagac
     6781 tctttctatc ctcaacaact ggaaattaca gcaaaaagaa gagtatttaa aacttggata
     6841 caatactagc tctaaagagc aacatgtttt tacaacagtt aaaaacactc tatacattcc
     6901 aaacactgtt aatgattggt taagatatat tttgaaaaaa tataacttgc ctcgtattac
     6961 acctcatggg tttagacata cacatgctag tttattatta gaagcaggag aatctgtcaa
     7021 ggtagttcaa caacgattag ggcatgaaaa tagtaaagtg acattagaca tctatgcaca
     7081 tattacaaat aatgcaccta agaaaacagg tcaagacttt gctgatatga tggctcatca
     7141 gtaaaagagc tggtcaaaat ctggtcattt ctagtataca ggcaaaacaa aaaagctaga
     7201 acccttatat atcaagggtt ctagctccta taacaggttt acccaacact gccttccaat
     7261 gactcttttg ggaatcagag gaaagaatca ggctttatag agatatttca tctaacaaat
     7321 tactttagac tgacagtata tatcagcagg tactgtcatc gggaacgtag ccaatatcgt
     7381 atccaaaaca gcaggtagag aagctatttg aggattcaag ttattctatt tcaaccatga
     7441 agttaagttc attaaaaata aagatagttg gtatttcaat aatttttgta tagagttaat
     7501 cactttattg atgctaattc atcttgattg gtattataat cgtattcaag ttgcgtggcg
     7561 tgcttttgct agaaaatata taactgttct ttataaagaa atataaggat taaacatttt
     7621 t
//
LOCUS       DAYYME010000125         7498 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000125 DAYYME010000000
VERSION     DAYYME010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.126"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            206..529
                     /gene="relB"
                     /locus_tag="ACHBFW_002342"
     CDS             206..529
                     /gene="relB"
                     /locus_tag="ACHBFW_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFM9318611.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            849..1076
                     /locus_tag="ACHBFW_002343"
     CDS             849..1076
                     /locus_tag="ACHBFW_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFM9318612.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            1092..2258
                     /gene="hemW"
                     /locus_tag="ACHBFW_002344"
     CDS             1092..2258
                     /gene="hemW"
                     /locus_tag="ACHBFW_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFM9318613.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            2493..3536
                     /gene="hrcA"
                     /locus_tag="ACHBFW_002345"
     CDS             2493..3536
                     /gene="hrcA"
                     /locus_tag="ACHBFW_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFM9318614.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            3630..4193
                     /gene="grpE"
                     /locus_tag="ACHBFW_002346"
     CDS             3630..4193
                     /gene="grpE"
                     /locus_tag="ACHBFW_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFM9318615.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            4244..6073
                     /gene="dnaK"
                     /locus_tag="ACHBFW_002347"
     CDS             4244..6073
                     /gene="dnaK"
                     /locus_tag="ACHBFW_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFM9318616.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            6224..7390
                     /gene="dnaJ"
                     /locus_tag="ACHBFW_002348"
     CDS             6224..7390
                     /gene="dnaJ"
                     /locus_tag="ACHBFW_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFM9318617.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         2567 a         1274 c         1664 g         1993 t
ORIGIN      
        1 acatcctcca gtaaattcta agtactggag gattcttttt ctagtgttgc tttactcatg
       61 aaaaagtagt tggctacttc atcaaaattt ctgaactttt ttaatgtata ataaagagct
      121 cgatcattta ctacaaggat gctagctcct acattttcac agttttttag caagtaaatt
      181 tttctaaaat cacatcagga aatgcatgag attctttaag agaggcaatt atagggctat
      241 gattacggtt cgtgtaagtg acgccaaaaa agaatggctt tcttatatgg ctgaatttta
      301 tgggattact ttatctgaac ttctaaaaaa ctattcgatg gaacaactag aagatgaata
      361 caatcgtcaa acggctgaaa ttgcccataa acattggata agaagtggga aaacaacagt
      421 attcatggaa gaatccttaa tgaatttggt gggctggaat gacctgccgt ttaaaattca
      481 cgcccgaaat tcagaagcag ctctgattgg caaatacgtg tattggtaga gatagaagat
      541 aatcaattcg ttgttatcgc tattcaagta gggcaccgaa gaaatgtcta tgattaagag
      601 actgtttatt tttacaaaaa aataataatt ggaatctagt taaaactctt gaagtgagag
      661 atttgactag atttttttat gaataaaata aggacaaaat tgtttaggaa gaatcatgag
      721 tgtaaaaaaa tcatccaaga agtcgattct tggatttttt agtggatttg ttcgccaaat
      781 acgtttagta tgcgtatatg agaggttgta agcaaagcgt tgtggtctga gcacttaagg
      841 aggaatctgt gaaaatagct tttcatattt ttgcagattt tgacttaatg ccgaaggtca
      901 gctttgcgat taccgtttat cgagaggctg taaaagcacc gtgtagctcc gatgacaata
      961 agcaagcatt tttagagcta ggtttccttg attctaaata tgcggcttat ttcaatgagt
     1021 tggtgcttga acgcgatttt aagaaggtcg aaaacaggca cgacttttcg acgtaaggag
     1081 aaagaatttc aatgatagaa acaataaatc ccgcagaaaa caaggtttct gcgtacattc
     1141 atatcccatt ttgcgagcat atctgctatt actgtgactt taataaagtt tttttagaag
     1201 ggcagccagt agatgagtat gtggaaatgc tgctgaatga aatgagatta atgctggaaa
     1261 gtcatcctac agcgaatttg gaaacacttt atgtaggagg cggaacacca acttctttat
     1321 ctgctaaaca acttgatcga ctattatcag gtgctagaga gatcctgcct tttcaaaatg
     1381 gtaatgagtt tactgtagaa gcgaatcctg gtgatttgac ccgtgaaaaa cttcaagtga
     1441 tgaaaaacta tggcgtcaat cgtttatcca tgggagtgca gacatttgac aatcgcttat
     1501 tgaaaaaaat tggacgtaaa catactgcag aagatgtgta tcaaacaatg caatttttag
     1561 aagcagaaaa ttttaccaat gtaagcatcg atttgattta tgcattaccg ggacaaacac
     1621 tagaaggata tcgggatact ttggaaaaag ctttggcttt ggatctgcct cactattctt
     1681 tgtactcatt gatcctcgaa aacaaaacaa tgttcatgaa ctgggtacga caaggaagac
     1741 tcgaactgcc tgatcaggaa acggaaacca gaatgtttga agagacgatt gaagcaatgg
     1801 aaaagcatgg acgtcaccaa tatgaaataa gtaactttgg gctctccgga catgaatctc
     1861 aacataacct catgtactgg aacaatgacc actattttgg gtttggagct ggtgctagcg
     1921 gttatcttgg agatattcgt taccgcaaca aaggaccaat ccaacactat ttacgcccat
     1981 tacgagcaaa tgaacgcccg gtattggaag aagaaaaatt gacacgaaaa aatcaaatag
     2041 aagaagaaat gttcttagga ctaagaaaaa aaacagggat tactaaaaaa catttctatg
     2101 atcgctatca aaaaacaatt gaatcggtgt acggtgatgt gctaagggaa ttagagcaag
     2161 aagggttact tgttaatgaa gaagaccgtg tttatttgac accaagaggg acttttttgg
     2221 ggaacaatgt gtttgagcgc tttttatttg aaaaatagtt tgaagaatat ttgtttttta
     2281 tcgaaacttg aaaagaaaaa gtttagaaag tttgacaatg aggtattgaa gtattgatat
     2341 aactggattt acatcaatta tttagaatta gcactcttgt gtttagagtg ctaaaaaatg
     2401 gagtctttct cttgacagga gaagggcttc ttgctatatt tataagtgaa ttagcactta
     2461 gaatatatga gtgctaatga gaggtgagga agatgttaac aaagcgacaa aacgacattt
     2521 tacgtctgat catccaacac tatacgagta gtggcgtgcc cgttggttcc aaaacattaa
     2581 tgtctgaggg agtcaaggcc agctcagcaa cgattcgcaa tgatatgaaa gcattagaag
     2641 atgaaggatt gctgttgaag actcactctt cttctgggcg gattccatct gctttaggct
     2701 atcgttatta cgtggatcat ttgttaaaac ctgctcgcgt agcagaagat gatttgctgc
     2761 tgattcgtca gtctctaggc agagaatttc atgaaatcaa tgagattatt aagcaatctg
     2821 ctgagatatt atctgagctg acaagctata cgactttttc attgggacct gaaataaaag
     2881 accgccgact gactggcttt cgtatcgtac ccttgaataa tcgacagatt attgctatcg
     2941 tcgtaacaga tatggggaat gtagaaagcc aagtgttcac tcttcccgaa tatatgggaa
     3001 gtcaggaact agaaaaaatg gtacagattg taaacgaccg tctagtcgga gatcctctga
     3061 tgacggtgta ccataaactt cgtacggaga ttccgatgat tcttcataaa tatttccaga
     3121 cgacagaagg gatgtctcag ttatttgaca ccgttcttgg tcatgttttt gaagaaaagg
     3181 tttatgtcag tggacaaatg aatctcttgg attatgagcc ccgtcaagat cttgatcagt
     3241 tcaaatcgat gttttctttt atgaaagact cagacggact tacacaaatg atcactccga
     3301 tggatagcaa tattcatatc aagattggtg ctgaattagg caatgattta ttgcagaata
     3361 tgagtatgat tcaagcaagt tacgagatag atggtcatgg aagaggtaca atagccttgt
     3421 tagggcctac tagtatgcct tattctcgga tgttcggtct agtcgatgtt tatcgaagag
     3481 aactagcagc taaacttgct gattactacc gctcgcttga cttgtcaaac tcgtaatgtg
     3541 aataacgaag ctatttgcag ctgatcgttc tctctttctt agtaagagca atcaaacagc
     3601 tgactgaata atgaaaggaa gactgtatcg tgaaagaaaa tcaagaagaa ctagataaag
     3661 aaatgacaga tgctcagcca gaaccggaaa tcgatgtcga agctgcagaa gattcgggta
     3721 tttcagaagc tgaagcggaa gaattcgaaa cagcgaaatt aaaagctgag ttagaagaaa
     3781 tggaagataa atacttgcgg gctcgtgctg aaatcgcaaa tatggcaaat cgaggaaaaa
     3841 atgagcgtga gcaattacaa aaataccgtt cacaagattt agccaaaaaa ttacttccat
     3901 caattgacaa cctggaacgt gcattagcca cagaagtgtc agatgatcaa ggggcaggtt
     3961 tgaaaaaagg cgtggaaatg gtcttggaaa gtttgcgtaa cgcactggaa gaagaaggaa
     4021 ttgaaaaaat ccctgctaaa ggagaagcct ttgatcctaa tcttcatcaa gcagtacaaa
     4081 ctgttccagc gactgaagat acaccagccg atacgattgt agaagtacta caagaaggct
     4141 acaaattaca tgatcgcgtg ttaagaccaa ctatggtcat tgttgcacaa taattgtgcg
     4201 gatataaaac ttacactttc aattaaagaa ggagacgtta attatgagta aaattatcgg
     4261 tattgactta ggtacgacta actctgcagt agcagtattg gaaggaaacg aagcaaaaat
     4321 catcgctaac ccagaaggaa accgtacaac accttctgtt gtatcattca aaaatggaga
     4381 aatccaagta ggggaagttg caaaacgtca agctgtgaca aacccaaata cgatttcatc
     4441 aatcaaacgc catatcggtg aagcaggtta caaagtagat gtagaaggta aatcatatac
     4501 accacaagaa atctcagcaa tgatccttca atatatcaaa ggattcgcag aagactattt
     4561 aggtgaaaaa gtcgaaaaag cagttatcac tgtgccagct tactttaatg atgcacaacg
     4621 tcaagcaaca aaagatgctg gtaagatcgc tggtctagaa gtagaacgta tcgtcaatga
     4681 accaactgca gctgctcttg cctatggttt agataaaaca gatcgcgacg aaaaaatctt
     4741 agtattcgac cttggtggcg gtacttttga cgtatctatc cttgaactag gagacggtgt
     4801 ctttgatgta ttgtcaacag ctggcgacaa ccatctaggt ggggacgact ttgataacaa
     4861 aatcatcgac tacatggtag cagaattcaa aaaagaaaac agcattgact tgtctcaaga
     4921 caaaatggct ttacaacgct tgaaagacgc tgctgaaaaa gcgaaaaaag atttatctgg
     4981 cgtatcaagc acacaaatca gcttgccatt tatcacagca ggagaatctg gaccattgca
     5041 tttggaaatg acattgacac gtgcaaaatt tgatgaatta acagctgact tagtagaacg
     5101 tacaaaagta ccagtacgtc aagcgttaaa agatgcaggt ctgtcacaat cagaaatcga
     5161 cgaagttatt ttagtcggtg gatctactcg tatccctgca gttgtagaag cagtacgtaa
     5221 agaaacagga aaagaaccaa acaaatcagt taaccctgat gaagtagttg ctatgggtgc
     5281 tgctatccaa ggtggcgtga tcactggtga tgtgaaagat gttgttttac ttgacgttac
     5341 accattgtca ttaggtatcg aaacaatggg tggagtgttc actaaattga ttgaccgcaa
     5401 tacaacgatt ccaacaagta aatcacaagt attctcaact gcggcagata atcaaccagc
     5461 agtagacatc catgtattac aaggtgaacg cccaatggct gctgacaaca aaactctagg
     5521 aagattccaa ttgacagata ttccagcagc tcctcgtggt attccacaaa tcgaagtaac
     5581 attcgatatt gataaaaacg ggattgtaaa tgtatctgcg aaagatctag gtacacaaaa
     5641 agaacaaaaa atcacgatca aatcttcttc aggtctaaca gacgaagaaa tcgaacgtat
     5701 ggtgaaagat gcagaagcaa acgctgaagc agataaagct cgtaaagagg aagttgacct
     5761 acgtaatgat gtagacgcat tgctattctc tgtggacaaa acattgaaag aattagaagg
     5821 caaagtggac gaagaagaag tgaaaaaagc agaaacagct cgtgacgaat taaaagccgc
     5881 tgttgaagca aacaacatcg aggaaatgaa aactaaacgc gatgcattga acgaaatcgt
     5941 tcaaaacttg actgttaaac tatacgaaca agctgcacaa caacaagctc aagaaaatcc
     6001 agaagcagct caaggcggag cagatgatgt cgttgatgct gattttgaag aagtcgatgg
     6061 cgacgataaa taatcagttc actgaatttc gagagaaggc caaagttgct actttggctt
     6121 tttcttttga aaaatagttt gaagtatcgg tttttgtttc aaacaaaata tgatatgata
     6181 acaagcgacg tgaaaatgat gagaaccaat aggaggaaag cctatggcaa caaaacgtga
     6241 ttattatgaa gtcttagggc tctcaaaagg agcatcagaa gatgaaataa agaaagctta
     6301 tcgtaagctt tcgaaaaaat accatcctga catcaataaa gaacctgatg cggaagaaaa
     6361 attcaaagaa gtatcagaag catatgagat tttaagtgac ccgcaaaaac gcgctgcata
     6421 cgaccaatac gggcatgcag gcacagatcc aaattacggt gctggtggag ctggtggcgg
     6481 atttggtgga ttcggcggct tctctggtgg cggcttcggt ggttttgaag atatcttcga
     6541 ctctttcttt ggaggaggcg gtcggtcagt cgatccaaat gcgcctcgtc aaggtgctga
     6601 tttgcaatat acggtcgact tgtcttttga agaagcgatt tttggtgtag aaaaagaaat
     6661 caagtataat cgggaagaaa tctgtcatac ttgtggtgga aacggtgcaa aaccaggcac
     6721 acaaccaact acttgtcata aatgtcatgg tgcaggtacg atcaatgtag aacgccaaac
     6781 gccacttgga cgagtgatga gccgtcaaac ttgtgatgtt tgtcatggca cagggaaaga
     6841 aatcaaagaa ccttgtccaa cctgtcatgg ctcaggacat gagaagaaag cacattcagt
     6901 taaagtgaat gtaccagctg gtgtagaaga aggacaacaa atgcgtttag ctggtcaagg
     6961 agaagctgga gaaaacggcg gaccatttgg tgatttgtat gtggtttttc gtgtagaaga
     7021 aagtgatatc tttgatcgtg aaggttctga aatttactat gaattgcctt taagttttgt
     7081 acaagcagct ttgggggatg aagtccaagt accaactgtc catggagatg tgaaattgaa
     7141 gatccctgct ggaacacaga caggtacgaa cttccgttta cgtggaaaag gcgctccgaa
     7201 gttacgtggt ggtgcaacag gcgaccaaca tgtcaaagta aaactgatca caccgaaaaa
     7261 tctaaatgaa caacaaagag aagcattacg tgcattcgct gaagctggcg gcatcaaagt
     7321 tgaagaacag caagaagacg gtttcttcga taagtttaaa gatgcttttg gcgggaaaaa
     7381 gcggaagtag tttttctagt aaaaatataa agtagacttg cccaaatttt ctatttctag
     7441 taaaaacgct aagtcattaa taattaaaaa ccagacgaac aatcatacgt tcgcctgg
//
LOCUS       DAYYME010000126         7496 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000126 DAYYME010000000
VERSION     DAYYME010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.127"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..1261)
                     /locus_tag="ACHBFW_002349"
     CDS             complement(29..1261)
                     /locus_tag="ACHBFW_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFM9318618.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1516..2085
                     /locus_tag="ACHBFW_002350"
     CDS             1516..2085
                     /locus_tag="ACHBFW_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9318619.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2263..2703)
                     /locus_tag="ACHBFW_002351"
     CDS             complement(2263..2703)
                     /locus_tag="ACHBFW_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM9318620.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2861..3625
                     /gene="map"
                     /locus_tag="ACHBFW_002352"
     CDS             2861..3625
                     /gene="map"
                     /locus_tag="ACHBFW_002352"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFM9318621.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3657..4580
                     /locus_tag="ACHBFW_002353"
     CDS             3657..4580
                     /locus_tag="ACHBFW_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM9318622.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4805..5044)
                     /locus_tag="ACHBFW_002354"
     CDS             complement(4805..5044)
                     /locus_tag="ACHBFW_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9318623.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5049..5468)
                     /locus_tag="ACHBFW_002355"
     CDS             complement(5049..5468)
                     /locus_tag="ACHBFW_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM9318624.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5695..6933)
                     /gene="mef(H)"
                     /locus_tag="ACHBFW_002356"
                     /gene_synonym="mefH"
     CDS             complement(5695..6933)
                     /gene="mef(H)"
                     /locus_tag="ACHBFW_002356"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM9318625.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7040..>7496)
                     /locus_tag="ACHBFW_002357"
     CDS             complement(7040..>7496)
                     /locus_tag="ACHBFW_002357"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318626.1"
                     /translation="FQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSVLL
                     TVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMVLS
                     NVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
BASE COUNT         2410 a         1387 c         1305 g         2394 t
ORIGIN      
        1 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
       61 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
      121 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
      181 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
      241 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
      301 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
      361 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
      421 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
      481 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
      541 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
      601 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
      661 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
      721 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
      781 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
      841 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
      901 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
      961 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
     1021 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
     1081 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
     1141 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
     1201 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
     1261 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
     1321 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
     1381 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca
     1441 tatcaaaaaa aacatgttac aatgaaatta atcaatggtg gaagcgttat aaaaaaatta
     1501 aggagattaa aaaatatggc aagaaaaaag acgattacta gagaacagat tttggcagct
     1561 gcttatgaag tagctgccac cgatggattt acacgattta ctgcgagaaa catcgcgaac
     1621 aaaatgaatt gttccacgca accgatttat cttgagttta aaaatatgga tgacttgaga
     1681 caagctcttt ttgagcagat ctatgatcat ttacaatatg aagtgttcac tgttgagcat
     1741 acagggaata cgattatcga tcttgctttt aattatatcc attttgcaag aagagagaag
     1801 aagttgtatc gttcactgta cttagaagaa tatggtggag gaaaagagat gcagaatttc
     1861 tcttatactt actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa
     1921 gagcagatcg aatctttgta taatgggaca tggatcatag ctactgggat cgcagctttg
     1981 atgtcttcaa gcatcatcca tccaactgat gaacagattg aaaagatgat ccaagacagt
     2041 atagatgcta tcttgaaatt caaagaacca atcgaaatcg attgaatata ctcaaaagtt
     2101 ccacaaatca gtaggtaaca agagatgtgt caaaataagg aaaagttcgt ttttcttaca
     2161 gcgtatgcat ttttaactcc cgaagaactc ttccatgaat gcaatgaata aaagagccat
     2221 acagattgaa taaatctgga tggctctttt attgcaagta tattaaattt ttgcgactag
     2281 ttctttagcg aaagcttcta aattttcgat atcttcttct tcagcattca gatccacttt
     2341 aacattttcg gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac
     2401 cgatttgcag aattcatcgt aaaaagtatc accagatccg cagacaccgt aaatttttcc
     2461 caatagatcc aattcttgca agtcttcata aaagtctacg atctcatcag gcagatcgcc
     2521 atcatcgtat gtataagtag ctactacgca aatatctgcc tcctcgaatt catctgcatc
     2581 tacttgggta cattcgttga tttcgacttc gatatccaga ttttcaaatg cttctgcaac
     2641 aatatcggcg atttcttcag tgtttcctgt catgctagca tatacgattt ttgctaaagc
     2701 catgatgtgc ctcctcgttt ataattattt aacaattatg aattatacca aaacatcatt
     2761 gaaaagaaaa taaaacctag agacacctgg ggaatgtcat ttggctaaat gtatgccgct
     2821 atgttaaaat gagagggatt tgtaaaaagg agacgaaaat atgattactt taaaatcagc
     2881 acgtgaaatt gaaatgatgg cagaatctgg agctttatta gcagatgtac ataaaaatct
     2941 gcgtgatttt ataaaaccag gaatcacaag ttgggatatt gaggtatttg tcagaaacta
     3001 tatcgagagt catggcggaa ttgctgcgca aattggcttt gaagggtatg aatatgcgac
     3061 ttgtatcagt ataaatgatg aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa
     3121 cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt ctgattcttg
     3181 ttgggcctat gtagttggag aatctacacc tgagatcgac cgattgatgg aagtaacgaa
     3241 gaaagctttg tacatcggta ttgagcaagc acaagtaggg aatcggattg gtgacatcgg
     3301 tcatgctatc caaacttatg tagaaggcga aaatctagcg atcgttcgtg attttattgg
     3361 tcatggcgtt ggtcctacga ttcatgaaga accagcagtt cctcattacg gagaagctgg
     3421 aaaagggttg cggttgaaag aaggcatggt tatcacgatc gaacctatgg ttaataccgg
     3481 aacttggaaa atgaaaatgg atcctaacgg ctggactgcg tatacaagag acggcggact
     3541 ttcttgccaa tatgaacata cattggctat tactaaagat ggtccaagaa ttttgacatc
     3601 acaaggagaa gaaggcactt attgatctat ccagtcaata ggtacgggag aggtacatga
     3661 aagtttggaa caaaataaaa caaaatgaaa atctgatgcg ctttattgag acgactcaaa
     3721 agcgcatcac tgattcagaa atgagtacga cctctgtcgt cgttgcttat tatttactct
     3781 tgtctttatt tccattgatt attgcttttg gtaatatttt accgtttctc cacatcgatc
     3841 aagagactgt actgacctat ttacgagaag tgattcctga gaccgtttac cagtttatcg
     3901 gtcctgcaat caaagacctt ttgacacaaa gttcaggagg cttgttatcc gtttctgcat
     3961 tagcggcttt atggtctgca agtcaaagta tcaatgcttt gcaaattgcg atgaataaag
     4021 cgtatggcgt agaaaataga aaaaattttt tgatcgtccg tttcttctca ctaattgtca
     4081 ttgtcttgtt catgattgct attagtgggg taactatcgt tcttgggttg ggacagctga
     4141 ttttggatgc tattcagcca atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa
     4201 ctttgaagtg gccaatcaca ttggtagctt tatttgtcat catgttcttg atttatttaa
     4261 ttgtccctaa tgctcagttg aagttgaaga cgatcattcc gggcacgatc ttcgctactg
     4321 tcggctggat gctgttatca caagtgttta gtatctatgc taggtatttt gcaacgagga
     4381 tcagcggtta tcagatcata ggaagtttta ttgtactcat gctttggctt aattttgctg
     4441 ctacgatcat cattttgggc ggaatcatca atgcagttgt tcaagaatac gtgacgggtc
     4501 atcaaatcaa aaaacgtcga gatccaagcc gaaaatggtt caataaattg aaaactaaat
     4561 tttctcataa aaaaaagtag aaacaagagt tcttattgta aatttatatc agtaatttat
     4621 ttgaaatttt atatctaatt ttcaattatc cgcataaaca ctatacattc aagcattttg
     4681 aagtatagaa aaacactcat cttaccacga atttaccacg attgaatgag ccttgatatg
     4741 accgtaaaac tactaaggga gatagcacga aactgtgtat ctcccgattt ttttatacag
     4801 ttgattactt cactccgatt tcaaatttca atattgcttt catcatgtct gtatgaattt
     4861 ctccttttag ttcttcatca acctgcataa tgattttacc actttcagag tggtaaaaag
     4921 gacgcaagca taatttagaa atataaccgt cataaaactt caaaatctct ttaactgcca
     4981 tttcatctcc gcttgacgct ttgcaaatca attcaaaaga gggatagcca taatctttac
     5041 tcatttcttt acttcctttc gctcaaaagc atttttaact tccttagtcc tctagttctt
     5101 ctactgcaaa tagcagaacg tgatacatgg tacagttctg aaatttctct gtcgttcatt
     5161 ccttgaaaat aacgtaataa aatcgcacta cgctgttttt ctgacagtct atgtaaaact
     5221 gtaccaagtg tttcatcaga tacataaatt gtaaagtgca agactttaaa tgatacacaa
     5281 tcataaaaac aggtatcaat cgttccacgt tcaacttgtt tcgtttcggc tatttcacaa
     5341 aacaatactt cacgtttaga atgtcttgca agctgtctgt ttcgactttt cactttgttc
     5401 ttaatgacaa tcagcattaa agaattgaac tgtaatctga cgatatgctc gaaagaagat
     5461 gtcctcataa tctcaccccc tttctgctaa tgctgaaata tataaacgga ataccgttta
     5521 taccctttag aaatacagaa aaatggtaag aaaaaagccc acacaaaaca tatatttgtg
     5581 cgagccttaa atactgcaat attactcttt cttttataac actatccttt tagcagattt
     5641 caaattttct tttatatatt gtttttagcc taacatacta tttaacccct tgtattatat
     5701 ctctaacttt tgaaaaattc tttttgaata tattgaaatc atgattgaag caatagaagc
     5761 tacaaacata acaacccatg tttcttgtat aaatacatcg aataaaatac cgtaaaccat
     5821 ttgtccaata ggttgaccgc acatagcaac tgctaccagc gtagccatta tctttcctac
     5881 taattgcggt ggtgtttgtg tctgaacaag agtgtaaatt tgaacaacaa acatagtaga
     5941 tacacccata gccacaaagc acatcactgt aactacccag taggaaacta ttgcaggcaa
     6001 ttctaaaaat agcgaaattc ccataagtaa aacagaaaca gaacaaataa gtaacaaggt
     6061 ataaaggttt tttagtttta actttgaagc aacaagagct gttaaaatac caccacataa
     6121 accacctaat gataatgctg attgtgaaaa accatatagt gtatctggca tttttaaaac
     6181 ttcagaaatt aagataggca gaccaacaat taaaacagca cttaaaacca aattgaaaat
     6241 gcaaaccaaa aagattacgg atataaaaat gggtttctcc tttttcaaaa aggtataact
     6301 atcttttaaa tcgtttttag caactgcaat tacacctact tcacgaggtc ttttttggtg
     6361 tggaatatgg ataaaaattt ccataatggc tgaaaaagta aaacatataa tactaacaat
     6421 taaaatggga ttgataccat aaaattgaaa aactactccg cttatagctg gtccgataaa
     6481 gtttgctaaa gtatttactt gatttattat agcattaccg acggtaagtt tatctgatga
     6541 taccaataga ggaatacttg cttgtaccgc tggttgatat gcaccagaaa taccataaag
     6601 taacatcaaa gcaacaataa acaaaggtat gataggaatt tttcccaatg acaaataaaa
     6661 tactaaaatt atgattgctg ttacaaaatc tagtatgacc ataatgtttc ttttatttgt
     6721 cctgtcagca ataataccac ctaccagcga caaaataacc ataggtaaaa aagagcaggc
     6781 tgtagcaata ccaaacaaag ctgatgaacc agtttctttt aacaaatagt acggtaatgc
     6841 aaatcgtagg atagcgtttc caaataaaga aattatctgt cctataacaa caagtataaa
     6901 atcacgatta aaaagttttt tttctgcttg cataaaatct ctccttttta tttaatcaaa
     6961 cagaccgtcg gtttatataa tggtttaaat aaactgctct tttatagagc agtctatcgt
     7021 atagggataa caaatacagt tatttacaaa actgtataaa tgcagaaatt gtttcttcgt
     7081 ctaataatgt gtctatgcct attccattta aaagggcatt gaatgtattg caacgttttt
     7141 ccataccctc tttatccgtc atttcaacta atgggttgag ccatacatta cttaatacca
     7201 tgataacttc tgcaatttca tgtgaatttt caatctccaa agacttatcc ttgatacctt
     7261 gttctaaaac gggttcaaca aattcaggta ctacaatttc atacatttgt tctatttgca
     7321 tagcaagaaa acgaggattg tttaatagtt gaggagaaac tgtcaataaa acgctttggt
     7381 tagaattgaa taccgctgtc ttaaatattt ctcttaactt ttctaaaccg tttaaactat
     7441 cgttatctct aattttagca aatattactg cattttcatt tccaattctt tgaaaa
//
LOCUS       DAYYME010000127         7424 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000127 DAYYME010000000
VERSION     DAYYME010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.128"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..1534
                     /locus_tag="ACHBFW_002358"
     CDS             143..1534
                     /locus_tag="ACHBFW_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9318627.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(1607..2254)
                     /locus_tag="ACHBFW_002359"
     CDS             complement(1607..2254)
                     /locus_tag="ACHBFW_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9318628.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(2262..2948)
                     /locus_tag="ACHBFW_002360"
     CDS             complement(2262..2948)
                     /locus_tag="ACHBFW_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9318629.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2954..4027)
                     /locus_tag="ACHBFW_002361"
     CDS             complement(2954..4027)
                     /locus_tag="ACHBFW_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9318630.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            4249..4923
                     /locus_tag="ACHBFW_002362"
     CDS             4249..4923
                     /locus_tag="ACHBFW_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9318631.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            4928..6268
                     /locus_tag="ACHBFW_002363"
     CDS             4928..6268
                     /locus_tag="ACHBFW_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM9318632.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(6417..7403)
                     /locus_tag="ACHBFW_002364"
     CDS             complement(6417..7403)
                     /locus_tag="ACHBFW_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFM9318633.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         2303 a         1382 c         1309 g         2430 t
ORIGIN      
        1 gctaaaggga atcacaccaa ttttcatacc aattggtttc atttatgaag ttgattcctt
       61 cgattaatga gcgtttcatt gaaagaaaag ggttgaggga gaaaaacata tgatcaggtt
      121 gttggaagga aactatagaa agatggaaaa aatcattcga attctgtcca tttctgacaa
      181 gtggatgact aaaaaagaac ttgccaaacg aattggtagt tcagaatcta catttgtaag
      241 gtatatcgaa gagatcaaaa tccgctgggg aacggatgta acgattcaat cttcacaaaa
      301 attggggtat cgtcttgaac atttgaatgt accagcttat ttaaatgttc tgatggaaat
      361 gtcgcaaagt tctacaaaca gtcgtttatt aaatgagatt attgtcaatc cgggtaaaac
      421 gattgagtat tattgtgaaa aaatttccat cagccggtct tcttttacac gtaagttgaa
      481 acaatgcaat caaattttag aaagctattt attaaaagtg atcgttgacc aaggttttca
      541 gttaactagc cttaaaagtg agctccagtt gcgtatcttt gttacctttt tctttttaag
      601 ttactacgga agacgcacgt tgccatttcc aatcgaaaaa aagaagatca aagaaattat
      661 ggtcaagaat aattgtcaga tgaatttgtt tcaggaagaa agcccatatg agcaatcttt
      721 ctttattatg tattttgttg tgcatctcat gagagaatcc caaggaactc catttgaatg
      781 gaaagctgga gactcttatg tacagttgat aaatatcgat aaaaaagact tcttaatctt
      841 gaaaaatctc ttaggaaaat tgaagtacag cagctatata aaagtagtga actattttgt
      901 caatacgatt ttttcagaat tttcggagaa agaaaaaaat gaaatcagtg agcagatacg
      961 aaaaaatatg tcctactgtt cgttttcatc cgctgattgc aaagaaaaaa acgaagaata
     1021 tgttgttcaa ttattaacgc aaatttattt tttctcccgt attttcccat acgatacgac
     1081 tcatttgacg atccgtgcaa cttactttgc acagcagatg caacaccgcc agatacgtct
     1141 atatgctgga tttcgtgcag aacttcaatt ttattccact ctactgaatc agaatctgat
     1201 ttattattta tctagctata tcttttggat gcttatcggt aatcctgaaa ttcaccatta
     1261 tacttctgat aaaaaaatac tgattatcag cgacttaagt cttcgacata gtcaatatat
     1321 tgaagaatat atcagtgata ttcttgctgt gcataaaatt cactctgaga caactgctat
     1381 tactgaagat cagctgtcga aatacaatct tctcgagtat gatctgatcg ttacgaatca
     1441 acccattttg aatgcccatg ttcctcatat attgattgat gatagtgtgt cattcgcaaa
     1501 tgaagaagaa ctcatccgct tattcgagtt atgaataatc aaaaaacagc tatgtataaa
     1561 ccaccctgat aaaaatttca ggacaaatac acatagctgt ttctttttat tgtattcctt
     1621 cagcaatcca acgagattta tagcgaaagt cttttaacaa ctcttctttt gtccaattat
     1681 ttgcaatgag atccgtcaaa gtgttcatta cgagggtcat ggaaaagtat aggaaactaa
     1741 ctgcttcttc cgacgattga atggagaaat gattgattct taataattcc agccattttt
     1801 cgttcattgg agaatcagcc gatagttctc tacgtttggt tgtctgctga tcatttcgct
     1861 gggttaacaa gtataacctt tgccaataga tactttcgtg atctaagtcg cagcaccact
     1921 ctaaatattt ttcgatcccc aaaaagaaat gttgtttgtt ttcgctaatg aattttaaga
     1981 tatcttgatg gactatgtga gaactgcttt tgatcgtata caaataagca tcttccaaat
     2041 cttgaaaata tttataaaaa gcaccgcgcg acatccccat cttgtcaacg atttctgaga
     2101 ctttgacttg gctgatgtgc tgtgagcaaa atatctccag taataggtca tcgattctct
     2161 gtttctttgc gttcgataaa ttgaaaaaag tttcttttgg catccacatc tctcctttat
     2221 ctctgattat acgtggatag cccttcattg tcatgaagga ttcattcctt ctttttctct
     2281 tcttctgtca agtacccatc ttccattcga taaacgcgat cactccattg aatcatacgg
     2341 acatcatgag taaccataat agtcgctttt tgtttctcat gagcttcttt aactagtaat
     2401 ttgacgactt catatgcatg atctgtatcc aaactagctg ttggttcgtc tgctaagatc
     2461 aagcttggct cattatataa agcgcgagca attgccactc tctgtctttc tccaccagaa
     2521 aggtctttag gataactatt tttaagtgac tggatatcta aagattgcag caaggaatcg
     2581 atttttgttg aatcttcttt ttttgaagta acttggtcaa ctagttcaaa ttgttccttt
     2641 accgttaaaa aaggaatcaa attagagctt tggagaataa atcctatttc tttaaagcgt
     2701 aaatctgctc gttttttttc tggaagctca gttagtgagt gaccgttgat cactatttct
     2761 ccggaagtcg gtgtttgcaa tcctcctgaa atggtaagga aagtactttt acctgaacca
     2821 gaaggaccaa tgatgctgac gaattctcct tcctttacat aaaaatcaat tgctttcaaa
     2881 gctgagacct ttgtatgacc agaaccaaat gttttggtaa cttttttcat cgttaatatt
     2941 tttttcattt ttattaacct cctattgctg taattgggtc tactttggtt atcgtacgta
     3001 tcgaaaacaa tccgcctagc attgcgacaa tgatcaaaat cccactatat agcaaccatt
     3061 gtgaccaaaa aacagcaaaa ggcatcgcgc ttggtaaaat cagactagtc aaataagcaa
     3121 acaaaagagc aagcagaacg cctaccaccc caacaataaa ggactgtgct actaaagatt
     3181 tagcgatgaa gaaatttttg atcccttgtg ctttcattac tccaaaaata gcagtcttct
     3241 gaagtgtcat cacgtacatg aaaataccga ccacggcagc aaccactaag aacaaaaagt
     3301 atatcatcgc atttaaggtc atattttggg cagaatagcc aggaatactt tcaatgaatg
     3361 tcccaatagt taataactgt agacccttcg atgctttttc tttcgaaatt ttagctgaat
     3421 cctttattac gatcccattg atcggctgtt cttcttcaga agcaaacggc tgttgtccat
     3481 acttcaaagc agtccacgta tctagggacg catagatgac gggagaaact gtgtaataag
     3541 tttcaggaaa gataccgaca atcgtcaaag gatcgtcgta actgccaatt ttgatcgtat
     3601 ctccaatttt atagcctgaa tcagccagat tttgagagat gatgatttca ttctttgttt
     3661 taaattcatg ccctttcgtg agttttggta ataaaaatgc ttctttcgtt gtgccgaaaa
     3721 ctgtaacatt ttctttgttt tttcctttga ttgctccgct gtacaatccg attggtgctt
     3781 tctctcctcc ttcaatgtat ttcagatctc ctcttgttaa ttgtgaagct gcaaatactt
     3841 gatttgcatc ctccgacaaa acaatctctt gtgcgtccca atcatcgatt gcttttttaa
     3901 actcttctga aagtccattc gctagtcctg acaacatgaa tacgacatac gcaatcaaga
     3961 acataatacc gacgatcaag ccatatcgta atttagaata gcgaatttct ttgatagcta
     4021 gaaacatttg tttttcctcc ctatgataga ttgatagtga caaaatgtca cttgttgaat
     4081 agtgtaactc taaagtgaca atttgtcact tgtgttgttt ttttcttatc aataaattgt
     4141 ctaagacatc tttcgataaa accttaacat gagtcataaa aaatttattt aagccttatt
     4201 tatgctttcc atgataaact aaagtgacca aatcaatgag gtgggaaaat gattaaacta
     4261 ttagtagtag aagacgataa agtattatcc aataatataa aagaaatact cgctgcaatc
     4321 ggcgagataa cacaagttta tgatggtagt gaaggactgt tcgagttgga aagtggagtt
     4381 tatgatcttg caatcttgga tttgatgatg ccggaaatga atggatatga agtgttagca
     4441 aaaatacgga aagaaaaaat acagactcca gtattgattt tgactgcaaa agatagtttg
     4501 gaagataaga ttgaaggatt tgaacgaggt gcagatgatt atctaacaaa acctttctat
     4561 cgagaagaac tgatcatgcg cgtgaaagca ttattgaaac gctcgctagg tctctttgat
     4621 gaaaatgttc tgaagtataa ggatatcagt tgtgatttaa aaagcaatga agtcttgtat
     4681 aaagaacaag ttttgcccat acaaggaaaa gagtttgaat tgctcgttta ttttttacaa
     4741 aataagggaa tcattttgac aaaagagcag atatttgacc ggatctgggg atttgattct
     4801 gatacgacga ttacagttat tgaagtttat atgagcaact tacgaaaaca tctgcgaagt
     4861 actggattgg ataaagaaat caaaacctta cgtaatgtcg gttatatact gcaaagggtg
     4921 tgatgcaatg aatggagata ttacacgaaa gcaacgtagt cgcttttttg cgacaaatgt
     4981 agcagcattt gcttttatct tttttctttt gggattcatt actttacaga tattaaacca
     5041 atctgcatac agagaaacag atctgtcatt agagaaaata aattttgatt ctcgaacagt
     5101 acaattagag atacaaagat atcagcaagg cgatcccttt ttaaacccga ctcaaacgga
     5161 acaaaacaga acaaacatgg aacagccatt aatggaacca tcaaatgacc gttttaacac
     5221 tcaagtgatc ttgtggtcta aaaatggtga gatattgaat aaagcagcta ttggtgggcg
     5281 tttcaaccaa atcaaagaat taaaaatgga tacgaaaaat ctgaatacca tccaaaatat
     5341 tgaattaaat gaagacagtg gctcatctgc cctctctttc cgttcgatta cgaaaaaagc
     5401 ggagacgaat caagatggcg ttgcctacat ccaaatttta gaaaatacaa atcaaatcaa
     5461 gaattcatta gcgacttttc gcacgattat gattttgtgt atgattgttt tttggattct
     5521 ctctattggt atcagttatt acttgtctag tctaagcatg cgccctatta ttctttcttg
     5581 gagaagacaa aaagaatttg ttgagaatgc ttctcatgaa ttgcgcacac cgttaacgat
     5641 tattcaaaat agtttggaac atctttttac caagcctaat catactatta ttgaagaatc
     5701 cgaaagcatc gctcaagcat tgtcagagac gaggcgcctc actggattga cttcggattt
     5761 gctgacaatc gctcggaatg attctgatca gcagctgctt tcaaagcaaa tgatcaatac
     5821 acaagaatat atcgaacaac ttgttaaacc atttcaagag atggccatca tagatggaaa
     5881 agagtttatt ctccataacg atgcaacatt agaagtacca attgatgaaa aaaaaatcca
     5941 tcaagttttg gttattttgc tggataatgc attaaaatat acgaatccag aagataagat
     6001 cattttggat tcatctgtta accataaaga atggctgatt cacgtaaaga atactggccc
     6061 ttctatcagt gaagaagatc aagcaaggat ttttgatcga ttttatcggg aagatcaatc
     6121 tcgggcgaaa gagactggcg gatacgggct tggcttggca attgcaaaac aggtaatcga
     6181 acagcatcat ggacagattt ctgtatccaa ttatcttcca caaggagtcg attttaaagt
     6241 ccaattgccg ttgaagccta aaaaataatg cttatcccta aaaaagggct gtgacaaaaa
     6301 tcttgtcaca gccctttttt gttgcgaata aacggtgttc cagaagtagc ttcttcggaa
     6361 ataagccgaa atttctttaa gctgaccact tctgtcacaa cctctttagc ttacgcttac
     6421 gattttttat tgcggtcttc aatgtgtaca tcaccgcttg tgtcaatatc caattcttct
     6481 cggctaacat cttctgtcac tttttcaaca tcttcatgtt cttctttatg aatcccaact
     6541 tcttcacgaa taactgtatg tttgcttaca tctacttgct cttctttgat tgggatagta
     6601 atcgtgtcat cttccaattt ttcgtctgtt ccctgtgaag attgatctgt tactggtttg
     6661 cgttcgatga cgatttcttc acgtttgacc ggtacttcta ccgtttcagt atcgttgacc
     6721 acatgtttgt ggatgtcgac ttctccagtt gtcacatcat gtgtatttac atctaatttt
     6781 tcttctttta gttgaattgt atcatcatct agatttggtt tgcgatcgat gtcttcagtc
     6841 ttgcttgtac ccattgtatc agtctcgcca ggtacgtcca tcggatcagt tcctggtgtc
     6901 atattcaatg gatccgttgt attttgaaag cctgtcgtgc cacctacgtc cattggatct
     6961 gttccagtgc cagtttctaa tccagtcccc attgtgcttg ctgggctaac agtatcttgt
     7021 gtatccattg tatcttctgt tgtttctggc tgacgatagc cttttactgt gatgacaaat
     7081 tttccatcag aaatatctcc tctatattga tataaaggat cattttctgg tgtagccgtc
     7141 tcagaatcat aggtatcaaa agaaaaagta tcttttattt tttcccataa agaacggtct
     7201 tcttcaccat ccatccgatt ttcatctgtt tcaacatcta taccagacaa tccgtcaaat
     7261 cctagtctag atgctgcttc atcagtagta taaataacga tatcatctcg ttcataccct
     7321 tcgttacgca accgttcaat tactgataat gcttcttccc tcgtactata gcttccgatg
     7381 accgttaaat ttttctcttc catgtaaatt cctcctagca aata
//
LOCUS       DAYYME010000128         7242 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000128 DAYYME010000000
VERSION     DAYYME010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.129"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..575
                     /locus_tag="ACHBFW_002365"
     CDS             <1..575
                     /locus_tag="ACHBFW_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM9318634.1"
                     /translation="LLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEP
                     KYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLI
                     PLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAA
                     AGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            587..1507
                     /locus_tag="ACHBFW_002366"
     CDS             587..1507
                     /locus_tag="ACHBFW_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9318635.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1548..3005
                     /locus_tag="ACHBFW_002367"
     CDS             1548..3005
                     /locus_tag="ACHBFW_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9318636.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3350..3964
                     /locus_tag="ACHBFW_002368"
     CDS             3350..3964
                     /locus_tag="ACHBFW_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM9318637.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            3957..5681
                     /locus_tag="ACHBFW_002369"
     CDS             3957..5681
                     /locus_tag="ACHBFW_002369"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9318638.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5678..7129
                     /locus_tag="ACHBFW_002370"
     CDS             5678..7129
                     /locus_tag="ACHBFW_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9318639.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2477 a         1206 c         1447 g         2112 t
ORIGIN      
        1 ccttactacc ttattttcta tcttgggtca ttatcagtta ctttgtttac gcttttttaa
       61 gtcctgacaa aggaatattc aatcagtgga tcactggaca tggaggagaa gctgtcaatt
      121 ggtataatga accgaaatat tggccgttta tcttggtgtt cattggtaca tggaaaggga
      181 ttggttataa cagcattatt tactttgcat ctgttatggg aatcgatccg acttattatg
      241 aagctgctat ggttgatgga gctagcaaat ggcagcagat caaacatgtg acgataccac
      301 aacttattcc actaatgacg attttaacga ttttagccgt aggaaacatt ttccgagctg
      361 attttggcct gttttataac attcctagga attccggtgc attatatgat gtgactcaag
      421 ttttggatac gtatatctac aatggcctga catccacagg agattttggt atgacggctg
      481 ctgcaggact ttaccaatca gttgtcggct ttgtcttgtt gatgatcaca aatacgatcg
      541 cccgacgttt tgatagcgat tctgcattat tctaaggagg aaaaaaatga aaaaaaagaa
      601 agtggagaga gtccaagtcc gctctttcaa taagacggct aattttttct ctaatataat
      661 gattgcactc tttgctattt cctgtatttt gccatttata tttgttatcg ccatctcttt
      721 taccgatgag tccatattgt cgactcacgg gtacagtttt tggccagaaa agttcagtat
      781 atttggttat acgtacttgt tcgaccagat gcaggataaa attttccaag cgcttttcgt
      841 cactgtactt gttactgttt taggtacact gatcaatagc acggctactt ctttatatgc
      901 ttatgcgatt tcacggtcaa actttccatt ccgtcgtttt tttaccgttt tttgtttaat
      961 tacgatgttg ttctcaccag ggatggtggc caattatctt gttatgacaa atcttcttca
     1021 gttaaaagat acgatttggg cattgatctt gccaatggct gtcagtccct tcaatattat
     1081 tgtgatgcgc acattcttta aacgttcggt ctctgattcg attattgaat cggcacggat
     1141 cgatggagct agcgaactgc gaatatttgt tcagatcgtt ttaccgttag ctgttccagg
     1201 tattgcgaca atcagtttat ttgccgcatt gggttactgg aatgactggt tcaatgcttt
     1261 actatacatc caagatgata agctagttcc actacagtat cttttgatga aaatccaaag
     1321 taatattcaa tatctgacac aaaattcagg agcaggaagc caattgacag gcggattagc
     1381 ctcagttcca ggagaatctg ccagaatggc gatcgtagtc atctcgacgt tgcctattgc
     1441 gatcagctat ccatttttcc agaagcattt tgtcaaaggt ctaacaatcg gcggagtaaa
     1501 agaataaaga aacaaaaata taaatcataa aattaggagg aagaaaaatg aagttatgga
     1561 agaaactagc ctttacaggt gtttcagcgt tgttgttggg aacattagca gcatgcggag
     1621 gttctggttc taaagaccag gcagaagcta gcaattcgga cactttgcag atgtatcaaa
     1681 tcggagataa accagataat ttcgatcaat tgatggaagt agcaaacaaa cgaatcgaaa
     1741 aagagatcgg tgtaaaagtc aatatcaatt acattggctg gggcgattac gaaaagaaaa
     1801 tgaacgtcat catttcttcc ggggaaaatt atgatatcgc ctttgcgaac aactatgttt
     1861 ccaatgccca aaaaggagcc tttgctgatt tgactgaatt agcgcctaag tacgctgaaa
     1921 aagcttataa tgatttagat gaagcatata ttaaaggaaa tctggtcaat ggaaaattat
     1981 atgctttccc agtcaatgga aatgtctttg ctcaacaagt acttactttt aacaaagctt
     2041 tactagataa atacgatctt tcaattgatg gaatccaatc ttacgctgat gcagaaaaag
     2101 tcttgaagga attccatgaa aaagaaccaa acacggcagc ttttgccatt ggacaaggct
     2161 tcaaagtcca agggaacttt gattatcctt tagggaatac gttaccattt gctattgatc
     2221 tggatggaga tgattcgaaa atcatcaacc aatacgataa tgaaacgtat atcggactgt
     2281 tacgtacaat gcatagctgg tacaaacaag gactcattcc aagtgacgct gcaacgagca
     2341 ataaggatta tccactagaa ggaaatactt ggctaatgcg tggagaaaca caaggaccgt
     2401 atgattatgg cgatacaatt ttgacaaatg cagcaaaaca agaacttgtc tcaaaagcaa
     2461 ttactgttcc actaaaatct acagctcaag cacaaatggc aaattttgtc gtatcaaata
     2521 cttcaaaaaa taaagaaaaa tcagtagaac ttctagggct gcttaacagt gatccagaat
     2581 tattgaatgg gcttgtttgg gggatcgagg gagaagcatg ggaaaaagtc gatggttcag
     2641 atgataaaat caaattgcta gatggttatc agccaaatac acatatgtct gcttggaata
     2701 caggaaacaa caagattcta tatacacaag agtctattac ggatgacatg attgccaaac
     2761 gtgatcaatc gattgctgat gcagaaactt caccgatttt aggattcagc ttcaacactg
     2821 acagtgtcaa aacagaatta agtaatattt ctaatgtcat gaatcaatat ttagatggat
     2881 tgaatacagg gactgttgat ccagatgaaa cattaccgaa attgaaagac gctttggaca
     2941 aggctggcta tgataaggta ttgaaagaaa tgcaaaaaca atatgatgag ttccgacaag
     3001 aataataatg aataaagaca aaggggttgt gacaaaagac gtgtcacaac ctcttgttcc
     3061 gaataaacgg tgttcaaaag ccagtttctc ggtattaagc taaatatatg taaaaatgtg
     3121 gaaagccata ttcacaaaat ttttcttact actcgaaact aagcggctat ttcacaaact
     3181 cttggtaaac ggtgttcaat cagctaaccc tcggtattcg ctcacaaaac gacaaaatat
     3241 gaggaactat ttttccgttt gtttctctaa taatcaggtc aaggtgctga ttgcccaacc
     3301 tttttttgta taatgagaaa gtataaactt aatgtttgga ggagaagata tgttagggaa
     3361 ggttttggaa gagttattta tccggatctg ggtcattata aaactaacct tgtatttctg
     3421 gatatataca tttgccggcg ggatcatctt tggattaggt gcggcatgga aaacagtcaa
     3481 tgaactattt tatttgtacg gattcgagta taaagaaatc acaataaaaa gaggctggaa
     3541 tatatataaa agaaattttt taagaggcaa tctgctattt agtttatttt tatctggtac
     3601 tgctttattg agttacaatt tatatttatc tgttcaaatc aaagggctca tttttttagc
     3661 tattgatttt attttacttt ttgccttgct ctgcctattt gctacttatc aatacagtat
     3721 gattttagat agtgaatata cgatctctgt tcctaatctg ctaaagctaa gttttatttc
     3781 ggttttttcc agcttttcgt cttttctaaa aataattatt ggtagcggta taattttagg
     3841 cgtgacttgg caattcaaag gattgatcct gtttggtgtc attggtcttt tgacggtttg
     3901 gaatggtacg atgacaacac attggcgtga agaattagat aaacagttgg aaagttatga
     3961 ataagtttcg gattctattt tcaaaaggaa ccttactaaa tcgtctgttg cggaaatacg
     4021 cggttatcat gatcggtgta acgatgactg ctaccgttat ttttagtatc catacttggg
     4081 aacaaaatca aaaacaggca gaaaacatga caagtgatgc cgttcaaagc acaagcagaa
     4141 tgctgaatga taaaacaacc ttgagtagga tcatcaaaaa ccagcttgta gggaattccg
     4201 aaaagatcga aaatgtgacg acctacttaa caaaacccat agatcagtat ctgatgtatg
     4261 tatacgaaca gcaaaacagt accgatgaat tagtttcttt cccaaatcaa atcaaagatc
     4321 tctacgcaaa ttatgaagaa ctaagtgcga tatatatcgt gttgaatcaa ctaccggagt
     4381 attatgaatc ttctagagaa aacaaaggtg gggaaataaa aaaggggaca ccaaaattac
     4441 tagacgcctt ttatatcaag ttgcctatca cacaaggagg agctgagtca ggaagtattt
     4501 ttattggttt tcaaaagaaa gaactagata tgattttaga aaatctaacg tcattcaatg
     4561 gactctcttt atacatgatt tctggaacga caaatcgact atacacattc catgataata
     4621 aaatagctaa agaaacgttt gaaagacaag aaatgatcat cacagagagt ctgaagaaaa
     4681 attcacaatt gccgattgac gatctggaaa aaaacaattt tgttcaacac caagaactgt
     4741 ctgatgatta tcgtatatta gctgtactag ataaaaaaag tgtaaaaatc gaaacgggaa
     4801 aaaatcttgc cccgctgatc attggtatca ttattttaga caatatactg cttatctttc
     4861 tttataaaac attcaaacga tattcacagc aagtatctat cgtcatgcag gccatggatc
     4921 aaacagcagc agggaattta gagacgcgga tcgacataac aaacacggaa tatgaactga
     4981 aggaactttc aatcggtatc aacgaaatgt tggatagcat caatcagttt gtagaagaca
     5041 tctacaaatt agaaatcaaa cagcaagacg cacatatgcg ggcgcttcaa gcacagatca
     5101 atccacactt tttatataat acattagaat atatccgaat gtatgcaatc agcgaaggaa
     5161 gtgaagaatt agcagatgtt gtgtatgcgt tttccgcttt gcttcgaaac aatacgaatc
     5221 aagagaaaac gatcacttta aaagaagaac tggacttttg cgaaaaatat gtctatttgt
     5281 atcaaatgcg ttatcctaat cgagtagcct atcattttat gatcgatcct gatttggaaa
     5341 agatcgaagt acctaaattt gtgatccagc cattagttga aaattatttt aaacacggta
     5401 ttgattttac tcgattcgat aatgcgctaa gtgtcaaagt cttgcaggaa ggcaaacggg
     5461 taaggatcat catcaaagat aatggaaaag ggatgactga aaaacggcta aaacagattg
     5521 aagaaaaact gtctcatcct aaagtggaac ttcacggttc tatcggtctg caaaacgtga
     5581 atgaacgttt acgtgccagc ttcggcagca gttattatat gtcgcttgag aataatgaga
     5641 caggtggact gaccgtttct attacattca aggagggatg agcgtgtatc aagtgttatt
     5701 ggtagatgat gaatacatga ttttgaatgg gttaaaaaag ataattgatt ggcaaagttt
     5761 aggctttcaa atagtagcaa cagcagagaa tgccaaagaa ggactagctg tacttgaaca
     5821 gcgacagatt gatttagtag taacggatgt gacaatgcca gaaataaatg gactggaatt
     5881 tattgaagcg gctcaaaaag aacgtcataa ttttgaattt atgattttat caggatacca
     5941 agaattcgat tatttgaaag gaggtatgca attaggcgcc gtgaattatt tgatgaaacc
     6001 cgtgaataag ttcgaattgg ttgaaagcct caagaaaatc aaaacacggt tagatcagca
     6061 aaatgaacaa aaaaatcaac aagaaatcta tcaagaactg ttattcagtc aatggttgaa
     6121 tgaagaattg gatgaaataa gtgaagaaga aataatagga caaataagtg agaagcagca
     6181 taggatagta ttgattcaac tggcaagaac tcatgatact cttatcaata gatggttgca
     6241 agaacggcaa caaatctttt actatcaacg aaattacgga gaccgtgtat tgatcacgtt
     6301 gttgttggag gcagatgaag ttgatggctt ctgttgtttt gttcaagaaa actttcctga
     6361 tcaggaatgg ctgatcagta tcggggaaga gacagattct ttggacaaaa tccctgaaac
     6421 attccaacaa gcgaaagaca atctacagtt gcatcaattt tatggagata aagacgagca
     6481 tgttttttac gcagtacaag cagaaacaaa agatcaaacc atcgattttt caagtttcag
     6541 acgcgtgctg caaaataaac aattggaagt cgctcaaaaa atgatcactg atttttttga
     6601 acagtttcag ttagcagcca tgatgccgga agatatacga tattccagtt ttttactgtt
     6661 tatggagatt caacgggaat tgattgatct ggaagatgag gaatatttac aaggaatcga
     6721 aaaaatccat caggcaaaaa cggtgcaaga acttcagcag ctacttctgt cattcatcaa
     6781 aaagcatcag agacagaaaa agtacagtga caatgtggaa aaagtaatcg taatcctgca
     6841 ccagcattat caagaaccac ttacactcaa agaagtgtca gaaaacctgc atttaaatgt
     6901 catgtatcta ggacagctgt tcaaaaaaga aacgaaaaaa agtttttcag cttatttgaa
     6961 ccatttacgt atggaaaaag cgaaacaact gcttttgcac agtaatcaaa acatcaatga
     7021 gatagcaagt gagattggct acaacaacac cacgtatttc tctaaattat tcaagaaaat
     7081 cgtcggcaga tcccctaaag agtaccgaga aaacagtgga gaatcataaa aaaaaaagcc
     7141 aatgtaatga aataacttat aaaaaattgt ctagaaatct aattttgggc tgttcttatt
     7201 acttgaggca aggcagcttt ttcatgacct ctcgataaac gg
//
LOCUS       DAYYME010000129         7013 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000129 DAYYME010000000
VERSION     DAYYME010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.130"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            185..1042
                     /locus_tag="ACHBFW_002371"
     CDS             185..1042
                     /locus_tag="ACHBFW_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM9318640.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            1035..1937
                     /locus_tag="ACHBFW_002372"
     CDS             1035..1937
                     /locus_tag="ACHBFW_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM9318641.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1970..2320
                     /locus_tag="ACHBFW_002373"
     CDS             1970..2320
                     /locus_tag="ACHBFW_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM9318642.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2369..3292
                     /locus_tag="ACHBFW_002374"
     CDS             2369..3292
                     /locus_tag="ACHBFW_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM9318643.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            3370..4263
                     /locus_tag="ACHBFW_002375"
     CDS             3370..4263
                     /locus_tag="ACHBFW_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9318644.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4301..4864
                     /gene="amaP"
                     /locus_tag="ACHBFW_002376"
     CDS             4301..4864
                     /gene="amaP"
                     /locus_tag="ACHBFW_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM9318645.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4878..5066
                     /locus_tag="ACHBFW_002377"
     CDS             4878..5066
                     /locus_tag="ACHBFW_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM9318646.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            5079..5981
                     /locus_tag="ACHBFW_002378"
     CDS             5079..5981
                     /locus_tag="ACHBFW_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9318647.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            6013..6255
                     /locus_tag="ACHBFW_002379"
     CDS             6013..6255
                     /locus_tag="ACHBFW_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9318648.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6511..6882)
                     /locus_tag="ACHBFW_002380"
     CDS             complement(6511..6882)
                     /locus_tag="ACHBFW_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318649.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2556 a         1190 c         1394 g         1873 t
ORIGIN      
        1 tggtcaatac ctttgaataa aaagccatga aaaacgaata gttagcgctt accgatgcgt
       61 tttgtgataa aaaaatttta aaatattcaa tgaaaacaaa gcatatattt tgctttaaaa
      121 ccaaatgagg aatattctag atgtagaaca ggttagaaaa agattagaaa aaggagtgat
      181 tttgatggag aagagatacg gcgtagttcc tgtggtaact gcttcagacg aaaattatgc
      241 tccttacttg agcgtgatga tcgcaactgc attagaaaat tgcaataaaa caagacgcat
      301 aaaattttat gtgattgacg atggtctatc agaatatagt aaagaaggat tggaagaaac
      361 ggtaaataaa tattcaagta atgcaagtat ccaattcctg acagtggaaa aagatatcta
      421 cgaagacttc ctagtcagtg accatatcac aactaccgct tacttaagaa tttctttacc
      481 aaacttatta gctaaagagg attataagaa agtcctttac ttagattcag atgtgttagt
      541 actagacgat atcgtaaaac tatatgatga gccgttaaat ggaaaaacaa tcggcgctat
      601 tatcgatcca ggccaagtga aagctttgga aagattagga atcgattccg atgacttgta
      661 cttcaattca ggagtgatgg tgattgacat tgatcaatgg aataaaaaag aaattacaga
      721 aaaaactatc cattacctca gtgaaaatgg cgatcgaatc atctatcacg accaagatgc
      781 tttgaatgct gtactttatg aagattggga acaacttcat ccaaaatgga acatgcagac
      841 ttcgctgatt tttgaacgtc atccagcacc aaatgaaaaa tatgaacgtc agtataaaga
      901 aggaaatgaa aaaccttcga tcgttcactt tacaggacat gataaacctt ggaatacgtt
      961 gaaagatcat ccgtatacca atttatactt aaaaaaatta gcacacagta ctctaacgaa
     1021 agtaggcgag atcaatgaat aaaaaagaaa tagcagtagt cgcaagttgt aacacaaaat
     1081 tcgtaccaca tttagctgca ttatttgtct cagtattaga taattgcaac ccgtctaaat
     1141 ttgttcggtt ctatgtgatt gacgatgata tcgattttga aagtaaacag cttttacgct
     1201 tctcagtgaa aaacgcacga atgaactcag atgtggaatt cttgaaaatc aataaagaat
     1261 tctttacaaa tgtggtaatc agcgatcgta ttccagaaac agcttattat cgtattgcga
     1321 ttccggaatt gttccgcggc acagaagttg aacgtatctt atatatggat tgcgacatga
     1381 tcgctttaca agatatctct aaattatggc gtcttgactt cggtgattca atcgtagcag
     1441 ctgttgaaga cgctgggttc catcaacgac tggaaaaaat gaaaatttca gctaaatcaa
     1501 tgcgttattt caactcaggt ttgatgctga tcaatgtgaa aaaatggtta gatgaaaata
     1561 ttacgcaaaa agtattagat ttcatcgaac ataatccaga aaaattacga ttccatgacc
     1621 aagatgcatt aaatgctatc ttacatgacc gttggttacc acttcatccg cgttggaatg
     1681 cacaaggcta tatcatggca aaagcaaaaa aacatccaac agcagcaggt gaacgtgaat
     1741 acgaagaaac tcgcaacaat ccttacatta tccatttttc aggacatgtg aaaccgtgga
     1801 gcaaggattt tgaagggcca acgaagaaat attacgaaaa atatgcaggg atgacggctt
     1861 tccgttgtgt agctaaattc cctaaatatc caaaatatgc aaaagttcaa ccaaaaacag
     1921 atgtagttgt taaataatag aagaccacaa ctgaagaggg ggagtaggaa tggaaattac
     1981 aatgtctaaa aatgcagtag agacgttaat tgaaaaggtt ggggaaaata caaaaatcgc
     2041 actcgcgctt atcaatgatt ccgatccatt tctaagggac aaaggtgctt ttgcaaaagg
     2101 cagctttttt caaatcattc cttttgtttc ggaatttgga gaatatgcga ctaaaatcga
     2161 gcatccatta ttggacatct atacatctaa attagagcaa aattatttcg gtaaacgatt
     2221 gaacatggac ttcaacaaac agcttgacag cttttcatta gaaaatgaaa tcgcagtact
     2281 cgattataat atcaaattaa agaactgctt cttcagctaa tagtttcgag aaagtccaaa
     2341 aaggaggtat acattgagat actactttgt ggatgaagaa aagaatgtaa tagaaacaga
     2401 tgaaaaaaaa tataattggc tcgtacttga tagtgcaaag cccgaagaga ttgagcaagc
     2461 aatcgaagaa ttcgagcttc ctgatgatat tttcgtagga accgaatatc cggaagaagt
     2521 ttctcggatg gagcgccttt atgagacgaa actagagcat ccattttctt tggtagtgat
     2581 taatctagat cagtcagata atcgtatcga aaaaaaatta acgccaatat cttttgtact
     2641 ttccgataat ctcttgatta cttgtatcga tagaaaaacc gaatttgtgg atcggctgct
     2701 gacaaagcat ggaaatcatt taaactcgtt tgaaaagatc atcacgtata cattattaga
     2761 tatttacacc cattacgtga aagaactaag agagatgaaa aagcgaattg acgctttgga
     2821 taaagcagct cgtaaaacaa cggaaaatga agaactgtat aaacaggcag atcttgaaag
     2881 agacatcgta tatatcgatc ataccttaag agatcaacgg gaaacaatgg atcaattatg
     2941 gaattctgaa actttcatca aacgtttaga cgatgaacag ctactctatg atgtccattt
     3001 gagacagcga caaacagaaa aaatgatcga gatctatcga gatttattag agagtattgg
     3061 tgatctattc aacgggatga tggacaataa tctaaatcat atcatgaaat atctagattc
     3121 agctgcatta atcatttccg ttccggcgtt gttcgcaggg atttggggaa tgaatacagg
     3181 aggattaccg ggtaaaagtt ctgctctagg ctttatactc gtagtctttg gctctttgat
     3241 agcagcagta gttttaggtt accatttata ccggaaagat tatacaaatt aaagaatcta
     3301 tttaaaacag gtatcaaaca aagaaatttg ttttggcaat acaatttaaa aactaggagg
     3361 aatacagcaa tgacagaacc aaaattaatc gacccgcgaa acttgtacca tacggagaaa
     3421 tttcctaaac aagatcagga tacaccagct ttacagcaga atatgattcc tgttcctgat
     3481 tgtggagaag aatcctatga aggcaataat caactagaaa accgccgtgt actaatcact
     3541 ggtggagatt cagggatcgg tcgtgcagca gcgattgcat ttgctagaga aggggcagat
     3601 atcgctcttc aattcttccc aggagaagaa aaagatgcag aggaagtagc aaaatacgtg
     3661 aaagatgcag gacgtaagat tgtcttacta ccttttgatc ttcgtgacga acaagcacct
     3721 aaagaaatca tagataaagc cgtgagtgaa cttggcgggc tagatacgat ggtactaaac
     3781 gcaggacagc aaatttcttg tcagtctatt gctgagttac cacttgagca agtcaaagac
     3841 acatttatga taaacatcat cgctatgttt gctttagtga aagaagctgt tccgcatatc
     3901 ccagcaggag gagcaatcgt aacaacaact tctgttcaag cattcaatcc aagcgaacat
     3961 ttattagatt acgcagcaac aaaagcttca atcgctaact ttacgatcgg tttagcaaaa
     4021 cagcttgcac ctaaaggaat ccgtgtaaat ggtgtagcac ctggtccaat ttggacacca
     4081 ttacaactag acgaaggaca accttctgat tcgatccctg aatttggtca gcaaagttta
     4141 ctagaacgtg caggtcagcc aggagaatta gcaccagttt atgttttctt agcatcgaat
     4201 aaagcaagtt atgttacagc ccaaatttac ggagtaactg gtggagaagc gatcaactta
     4261 taagaagttg atcgtttcac ccctatgagg aggtgcgaaa atgaataaag gtgtaaaaac
     4321 gatcggagtg atcggaacat tacttttgct atctgtgctt ttgtttgcga tactattaag
     4381 tgcaacctat ccattgcctt ttcagctaga gaagtttcga ttcttttcca tcactaattc
     4441 atacgtgcaa caatatgtat tttggcttgc cgttgcattt gctattttga caattatcac
     4501 gttacttgtt ttcctgtttt atccaaaaac aatcggaaac tttctactga aacatggtga
     4561 tggggagttg acgttggata aaaaagcaat tgaaggaatg gttcgttctc acttgcatga
     4621 agatgaattc atccattcac caaaagtaaa tgtcaaagca acgaaaaaca agatcgccgt
     4681 caatgtaaaa ggggagttga aacgaacttc agcactcatt ggtaaaacag gaacattgat
     4741 gaaagaaatc gaaaatgaag tacaacacat attgggaaca aaagagccag tgaaagtatc
     4801 ggtgaaatac acaggctttg aagaaccagc taaacgaagc aatgaccgca aacgagtaga
     4861 ataggggggg accaaaaatg aaagaactat tttcgacata caagctccct atcatctttg
     4921 gggtacttgg attgatatta gccgttttat ggatcaccgt gggctttttc aagaccttat
     4981 tgatcttgat catggcagcg atcggcgtcg ctatcggttt ttatctgaaa caaacgaaaa
     5041 tcttagatca ctattttaaa tcttaggagg aaacatccat gaataacaat aacaaaaata
     5101 ataacaataa aatgaacaac caaaaaccag tgtctgcaca tccagaccca acgacaccaa
     5161 accaatcaaa tggtgtgaac gatgaaaaga acaaaacagc accagtatct gcacatccag
     5221 atccagtagt ccctaacgtt caacaaggag tagaagagaa gaaacatgat gcagaaaagc
     5281 atgcacctca tcatccagga actccagaat ctaaagttcc agaacaacat gatgcaatcg
     5341 atccttctaa acctgtggaa gagcaaaaac atccttctga ccatgcgaaa gcacaagaaa
     5401 aacttagttc tgaccacaca aacggacaag gaaaacttgg ttctgatcat acaaataacc
     5461 aaggaaagca tgattctgaa caggaagatt caatgcgcgg tgagctgaat tatgaagata
     5521 aagttgtgca aaaaatcatc ggaatcgcaa ttgaacaagt agacggttta ttatctgcaa
     5581 acggtggttt cttctctaat gtggcaggaa aacttgtgaa tacagataat gtaacggctg
     5641 ggatcgaaac agaagtcggt aaaaaacaag tagcagttga tatggatgtc attgtggaat
     5701 atggcaaaga tatcgaaaaa atctttgaag aaatgcaaga agtaatcggc aaagaagtaa
     5761 aaaatatgac acatttagaa gtgatcgaag tcaacgctaa cgtagtggac atcaaaacaa
     5821 aagaagaatt tgaaaaagat caagaaacgg tgcaagacaa agtaactgac gcagcgaaaa
     5881 aaaccggtga atttgcttcg aatcaaacag ataaagtaaa atcagcagct ggaagcggcg
     5941 cacaaaaagt aaaagaaaat acagaaccac gggttcagta ataaggaagt aaaacattag
     6001 gaggaacgaa acatgggatt tatttggtca cttatcgtcg gaggaattat cggagcaatt
     6061 gcaggagcaa tcacgaataa aggttcttcg atggggatta ttgcaaatgt cgtagcaggt
     6121 ttagttggat cagcacttgg tcaagcatta ttaggttctt ggggaccaag tattgcagga
     6181 atggcaatcg tgccatcatt gattggtgcc atcattttag tactaatcgt atcattcttt
     6241 gctcgaaaac tgtaaaaaga cttttaaaaa gaggtcgtga aaaagctgtt cttcatcaag
     6301 taataagaac agccaaacaa aaatagcttc tcatattttt catttggttg ggcatattat
     6361 cgaagatgca agcttttgaa cactgtttat acggaataag aggctgtgac aagacttttg
     6421 tcacagcctc ttatttatgc gggcaaattc ccaattagat ttttaaaagt ttttccttaa
     6481 atgtgtcttt attgatacag agaagcttct ttaataaaac aatgcctccc taaattttct
     6541 tgagatagga aaggtgaaca tacaaaataa tacgaccact ttgaaaaccc agatgatcca
     6601 caaatggatc gtatgatact gatgaagtaa aaagcaatgg atcagaaact caaagagatt
     6661 gaaaaagaca aggaagaaat aatatctctt ttttactctt gtctgtttta gccaataata
     6721 gctcgtaaag taatctagac aaacaaatag aagtaaacaa agtttcgtaa gagcagcaat
     6781 cgtatcatgt tgctctgcta gacgattgac gagcaggaaa aatgttggta atggaatata
     6841 gatcacgagt aatgaaagaa aaaagtaaac aataaatggc atactattcc tcctaattgt
     6901 gtgagttcgc cctcatttta aaataacgat atttgtttgt gaagcttttt cgtgtcgtaa
     6961 caaatgttat ctaatgtatt tttaaataaa taatcattat tatcaaaata atg
//
LOCUS       DAYYME010000130         6976 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000130 DAYYME010000000
VERSION     DAYYME010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.131"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            27..464
                     /locus_tag="ACHBFW_002381"
     CDS             27..464
                     /locus_tag="ACHBFW_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318650.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            559..1443
                     /locus_tag="ACHBFW_002382"
     CDS             559..1443
                     /locus_tag="ACHBFW_002382"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM9318651.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1667..4834
                     /locus_tag="ACHBFW_002383"
     CDS             1667..4834
                     /locus_tag="ACHBFW_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFM9318652.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4902..5399
                     /locus_tag="ACHBFW_002384"
     CDS             4902..5399
                     /locus_tag="ACHBFW_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFM9318653.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            5418..5882
                     /locus_tag="ACHBFW_002385"
                     /pseudo
     CDS             5418..5882
                     /locus_tag="ACHBFW_002385"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            5879..6637
                     /locus_tag="ACHBFW_002386"
     CDS             5879..6637
                     /locus_tag="ACHBFW_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM9318654.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6723..>6976)
                     /locus_tag="ACHBFW_002387"
     CDS             complement(6723..>6976)
                     /locus_tag="ACHBFW_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HFM9318655.1"
                     /translation="VMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQK
                     IDSAHYVLEFFNGDSLLVGRKIFEKTKERFHDFQKTASS"
BASE COUNT         2347 a         1208 c         1529 g         1892 t
ORIGIN      
        1 caaaaaatgc acgtagaagt agataaatgg agtggttttg caaatttctt ttatttctag
       61 aagttattca cgtatacgga tttgtttttc tcacaaataa aaaggcagga agtacaatgg
      121 atcatttgta tataaaatat attcatcaat tttccgtttt ggtgttaggc acactaatta
      181 tagcggggat tgtcgcattt attattcaag gccgtgacat attaacagta catcaagagt
      241 ttttcagtta tgcatttggt attcctctga tcgctataag gtctgataac aaaaagtgga
      301 agaaacgcta ttacattctc ttgctatttt tgtgttttta tctgccacct aagattttta
      361 ctttattagg tatacaatgc ctatcatttt cttttatttc ctatatcatt gttttaattt
      421 tcctcagttt catagtatgg atgttttatc ttgtcaaaaa ataaatgaat gtttcatgaa
      481 cagtacttca tcacagccta atttttttct ttatgctatc gttatattaa agaaatcaat
      541 cagaagggaa agaaagtgat gaaaaaagtt ttattagtgg taaaccccag ttcaggcggt
      601 gaacaagcaa aggaatttga gcaattagcg atagcaaaat tggaatcggt gtttgatgaa
      661 gtggtcgtct tgcatacaaa aaaagcaggg gatgcaaaaa actttactcg cgaagcggct
      721 acggaagggt atcatagtgt gtttgtcatg ggcggagatg gaacagtcaa cgaaggaatc
      781 agtggaatcg ctgaacaaga acatcggcca aatttcggat ttttcccatt gggtacggtg
      841 aatgacttgg ctcgagcatt agggataccg ctagaacctg aggaagcgat caatcatttt
      901 tcgattgaat cagtcaagcc attggatatt ggaaaaataa atgacgacta ttttatgaat
      961 gtcgtcgcaa ttggctctat ccctgaatca atcaatgatg tagatgcgga gaagaagacg
     1021 aaatttggaa aactagctta tttcatgtca ggaatcaaac agttagcttc cacacaatcc
     1081 tactcgtttc acgtagaagt tgatggaaag aaggaagaaa ttgagagcag taccttgctg
     1141 ataggactaa ctaactcagt cggcggtttt gaaacattac ttccaaatgc aaaagtggat
     1201 gatggtaagc tccatttggt ttatttaaaa gattcttctt tgatcgatac gcttaaaaca
     1261 ttgccagacc tattaaaagg agtagatgaa tctacgaaaa atctagccta ccttacttgt
     1321 gagcaattag ttgtgtcatt agctgatgag gaagaattga cgacgaatgt tgatggagat
     1381 gaaggggata aattacctgt gacaatcaaa gtattgccct cacatttaca ggtttattgt
     1441 taaaatggag gttgtgaccg aggagttggc gggagaacac cgcttgttcg ggataaggga
     1501 ctgtgacaag acttttgtca cagccttttt tttgtacaaa aaagaaattt acacctaata
     1561 gaaaaatttt tccataaaaa tacatatttt aaatatgaaa gtacaattgg aaatagatta
     1621 aaacgataaa ataaaattgt caaaacgtca gagaggatgg tggagaatga atcaatgtgt
     1681 aaaaaagctg attgcgtcga ttttgtttgt cttccctctc ttgatggtga catccgtttt
     1741 tgccgatgta gagaaaacaa acgaatcaac gcaatcagag aatcaaagtt ttgacaagac
     1801 tactttttca gaagaatcga ttacttctga aactacagaa tctcaaagtg aggcttctac
     1861 taataaaagg caagaagaaa ataagacaag tgagacctca gaagtagctg aacaaacgtc
     1921 tgatagcagt gaagcggcac tggcaagagc tgtttcagcg gatgggtaca gcgaagctgc
     1981 cacaacagaa gaactggcgc agcttctcgc agacgaatca gtaactaaga tccgactgat
     2041 tcagcctttg actttagaca gggaattaga gatcaaacga gatatcgtta ttgattttgg
     2101 tggattcgct cacaatttcg gcacacacca tatttatatc aatgagatac ctcagtcgat
     2161 cgagttccag aattttaaag gaacagctgc acatccaggt ggattgacac caaatatcga
     2221 tggaaatgcc attatcattg cttatatgac cgatttttgg ggaagcagat atcattttac
     2281 gggagaaata aaatttaccg ggacacttga tttgtatgat gggtccaaat taggactgat
     2341 ttacgctcct cgtgcaactg tcacattaga tggtgtttcc ggtgtgttgg atgtccaacc
     2401 agtgaaggaa ggactgaatg ccgctccggg taaagcctat ttttgtagaa gctatcaatt
     2461 gaatgtgatc aatggtagtc aactttatgg accgtatcta gggaaattct acggttttct
     2521 agattcagga gatgagactg gtagtggaaa aacagcgcca ggaatccata tcttaagcgg
     2581 ttcaaaggtc tctcttgatt atgatcgggc agatggcgtt agcgatgaag gagaggcagt
     2641 cgatacgcta cctggtaatg tcatgtttga agtcagtggt tcgggaagcg aattttcggt
     2701 aaatacaaag atcaatatta ccaatgacaa caatcgcgga atcatccaaa tgagaggttc
     2761 aggatcgaaa gtcactgtat cgaatggtgg gaaaatcacc atcaatacag caactacggg
     2821 cggatttcgc ttgcaaggag attctagtca aatccatgta tcaagtggtg gacaaatctc
     2881 ggtcaaaatg gctggagatg acgatcaacc cgggaacaat gggatgcgtt ttgtaggaaa
     2941 aggcctttct ttgatcgtag aaggagccgg atcagttatc gacatcgata agcagagtgg
     3001 gcgttccagc gcagttcgtt ttgagaatgg tacgcaaaag ctgacagtaa ccaatggagg
     3061 ctcattgagg gtaagaaata caggtgatgg caaatcttac gtgaacagac tcaatcaagg
     3121 aaatcaagcg atccaatttt atgacgcttc tggttttcta gaaagtccag gttcagctga
     3181 ttttacagtc actggagaaa aatcaaatat cgatatacgc gcagattccg gagcaactgt
     3241 agatacaact ggagatattg atttaaattt tacggcaggt gaaaaaactt atttagtcat
     3301 ggtcgggaaa acaggggaag ataagaccgg catcatcagt ggggatgtgt tgaatgtgga
     3361 cttagaatct cctacctatt ttgatttcca aaacaaacgg attggaagcg agaccaatgg
     3421 cataggcggc tgggtattcc aaggaaatgg agattcttct ttaaagatcg cccattcaga
     3481 aatcgcttta tggcgaaaaa caggcgcaac attcgataac tttgaaaaag acccggatta
     3541 tttttctgat atgacagact tatcgatcaa tgggaccgac ttaggcaatt tcgtatcaag
     3601 ccctagtgca ggtttaacaa atgaatttgc tcagacaggt gccggaatga aaaattataa
     3661 tcgaatcagt gccaataatc agctcccgat gattgaatcc ttaagagtgc caacgaatgc
     3721 agataaaaaa atctatgggc atgtcgttgt tcccgaaggt gtcgaggcaa agccaagaga
     3781 tgcttggaca ggcgaagtag aagtgacttt acgtattact tacgcagata cgacgaaatc
     3841 atctgtagaa attactgctc tcacgcaagg acaaacagat gaaaatgaag gggcaggata
     3901 taacccatgg ggagaaggcg aacaaggcgg cttgttcgaa gttcaagtac ccaatgatga
     3961 gagattaaaa acggaggatc aagtgcaagt tgttgctgct aaaaaagtaa gaggtggcca
     4021 agaagtgaca gaactggact ctgccaaaac gacagtcgat gtgattcctc ctgaacctgc
     4081 taatttaagt accacattga tcaatgcagc ttctcgcacg atcacaggta caggtgaagc
     4141 aggagccttg gtttcattga aaaaaaatca agctgttttg tcagaaacaa ctattgatga
     4201 tacaggggaa tttgttcttt ctatcccaga aaatcaactt tttgcaggag atgtattaga
     4261 aattgtatta aacgaccaag caggagaagc tactgctaag ggtgtgcaga acaagccaag
     4321 taccaacgat gaaattggga atcacaatcc aagtgatacg ccagttgttt atcatgatgc
     4381 cccgcctttt gcacctgcta cgaagatcgt agtagaaggt ggtctctcat ttcttgcccc
     4441 ttccaaactc gattttggtc agatcaaagc aactggtttg acacaagaag catttggcca
     4501 aatggatata caggaaaaat tatggattta tgatcagcga gaaacgaaaa atccgtggat
     4561 cttaacgata aaattatctc agcctttcac aaatgatgca ggattttcta tgcaggatgt
     4621 cctatatttc aagaaaggag aggagtatca attagtgaat gaagaagaac tggaaattgt
     4681 cagaggacaa aattcaacga atgtgcaagc agattattca gaatatttaa ataacggtcg
     4741 ggcgtttaaa cttgtcttaa ccaaagaaaa tcagatagct ggtaattact cagcagagat
     4801 aacatggaca ttagcagatg cgccgacaga ataaagatca aggaggatga ttgatgaatc
     4861 gaatatggaa taaaagtcat cgaaacaact gctctttact tatgatcctt ctccttggat
     4921 tcatcctttg gcagacacct gtccatgcct ccgaattcaa ttttgcggta gattttgaat
     4981 taccggctaa tcagattgat aagaacaaaa catatataaa tgtaaggatg gagcctggga
     5041 aaacacagga aatcccctac acggtcttta accgaacaga taaatcagta acagttgaga
     5101 cattgatcca ctccgctacg acgaattcaa acggtgtgat cgaatatgga gaatcaaaaa
     5161 gtaaatttga tgaaacatta agttatgata tgaaaaactt agcaaaaaca gtgggagaaa
     5221 taactgttcc tcctcaaagt gcggtcactg agacactttc actgaaagcg ccagaaaaag
     5281 agctggctgg tgtggtcgca ggagggatca cgtttcaaga aaaaaaagag aacgtttcta
     5341 acaaaagtac agttggtgtc gtcaatcagt tttctcaagt gaaagcaatc ttgatttgaa
     5401 atagcgaaaa agagtcttcc ccagatttga aattaaaaaa agcttatgcg gatcaaagcg
     5461 atggaagaaa tgtgattaag gcgaatcttc aaaatccaaa tgcttcttat atgttcggct
     5521 tgactacatc ggttgaaatc tacaaaaaag aaacaaaaga gccttacctt tcaataaaaa
     5581 aagaaaaaat agatgtagca cccaatacaa attttgattt acttgtccca ttagaaggaa
     5641 aaaaattagc tccgggacat tatcaagcgg ttgtgaaggg tgcttggaaa gaaaaacgct
     5701 ggaatctcca taccgatttt gaaataactg atgatgcagc ggaccaattg aatgaaaagg
     5761 atattgattt aaaaaatcag ccagaaacat ttgactggcg atggctgata cttgttggag
     5821 tcattcttat tggcttattt gctggattat ttattttaaa agaaagaagg aaaaagaaat
     5881 gaaaaaaaca attgcattaa gtaccgttct agctggaagt ctattattat gtacacaaag
     5941 tgtttttgct gagaataaga gctcagccac tacagaagga acgatttctg tgaccaaacc
     6001 agatgctgca aatcctgaag acgttgttcc agttcctcct gttgatccaa atgatcctga
     6061 tactcctttt acacctgacg aaccaaatca gggaacatcc ggattactga cgattgacca
     6121 agctccagat cttgattttg gaacaatcaa gttaggtaca gcaaaaacag tatatgctgc
     6181 attacaaagc ggaacggaca gtactggagc acaaacacaa gtacagaatt tcgtacaagt
     6241 aacggataaa agcggaaatt atgccggttg gaaattatca gtcaaacgaa cagaatttat
     6301 tggtacaaca tctgatacga ataaactaac tggttcaaag gtgaaattca aaaacgccgt
     6361 gattcgaaat agcgcaacta atagcagtgg aatgccatct accgtttctg gaactggcgc
     6421 tgaaggaatc gaaatcccag cagatcaggc agtagatcta gtaactgcag gagcaaatga
     6481 agggattggc acatggattt attcattagg agagaatgtg gaacaaggga gacaatcagt
     6541 ggaattattt gttcctgtat ccaattatgc tgtagacaac tacgtttcaa cattcacatg
     6601 gtcgttaaca gatgctccta cagaagagga agcgtaaaat catatcaacg atttcaggga
     6661 gtggagccga ttcaaggccc actccctgaa atctgctatg cttacgtctc agctcttatt
     6721 catcaactcg aagcagtttt ttggaagtca tggaaacgtt cctttgtctt ttcaaagatc
     6781 tttcgaccaa cgagtaagga gtcaccgttg aaaaattcta acacataatg agcagaatca
     6841 attttctgca attgcgataa gttgatacag taagatttta aaagtaaaaa gtaagcatac
     6901 ttttctaact ctgtcttcaa ttgttttaaa gaagtattca ctaatttatc ttctgtagtt
     6961 gttttgacca taactt
//
LOCUS       DAYYME010000131         6662 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000131 DAYYME010000000
VERSION     DAYYME010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.132"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..527
                     /locus_tag="ACHBFW_002388"
     CDS             <1..527
                     /locus_tag="ACHBFW_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318656.1"
                     /translation="FYLFMTVLLLITLTVSSKKNFNQKIISQPGHIVVNKETVEAYTT
                     KGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVIT
                     LPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLT
                     AYLCLFFKIVFHKR"
     gene            702..1535
                     /gene="msrA"
                     /locus_tag="ACHBFW_002389"
     CDS             702..1535
                     /gene="msrA"
                     /locus_tag="ACHBFW_002389"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM9318657.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            1615..2604
                     /locus_tag="ACHBFW_002390"
     CDS             1615..2604
                     /locus_tag="ACHBFW_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM9318658.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            2893..3591
                     /locus_tag="ACHBFW_002391"
     CDS             2893..3591
                     /locus_tag="ACHBFW_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFM9318659.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            3741..5057
                     /locus_tag="ACHBFW_002392"
     CDS             3741..5057
                     /locus_tag="ACHBFW_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9318660.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            5074..6489
                     /locus_tag="ACHBFW_002393"
     CDS             5074..6489
                     /locus_tag="ACHBFW_002393"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9318661.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
BASE COUNT         2261 a         1048 c         1392 g         1961 t
ORIGIN      
        1 tattttattt atttatgact gttttgttat taatcacttt aactgtttct agtaaaaaaa
       61 atttcaatca aaaaataatt tctcagccgg gacatattgt tgtaaacaaa gaaacagtag
      121 aggcgtatac cactaaagga atggattatt ttttatatga ttattctcct atcgcttcat
      181 tggagaataa ggagcatata ataaaacacg aagcaaagat tggcagtcaa tgggtaaacc
      241 ttccttataa aacaactggt agttctatca tatatagagt aactaatgag aagagccaag
      301 taatcacact tcctgtatat gcttatttag gaacgaatgt agaaataaat catcaacctg
      361 taaaggagca ggtgaatgct aatggattgg tacaaataag cggagtcaaa ggagttgctg
      421 atattaaagt aagcacaagt tatacagtaa cgactattat ttcttatctt gttacattaa
      481 ctgcatattt atgtttattt tttaaaatag ttttccataa aaggtaaatt aacttttcat
      541 ttataaaata aattgattaa aaaggagaat ctgtcgataa ggattctctc ttttttatac
      601 gaaaaatctg tagggggatg aagtaatcgc ttatttcctc actttatgat aaattacagc
      661 taaagtcact agcttgtctt cttcggaaag gaggagaatc tatggatgag gaacaaatat
      721 tcattcaaac actttataat ctgattttga atccgaatac acgagattgg gaacgaaaag
      781 tattgattca gacaaagaat gacataagag aaaatatatc cgttaaagaa cagttatcaa
      841 aactagaagc aacactacgt ccattagcta tccgaatgaa tctgacgcct gatgtaatgg
      901 acttttattt attgattacg gaaggatttg ataaggagca aaaatatgac ttttccaagc
      961 atgccatgca agatgctgac tatcaagaac gtgcagtatt tgcaggggga tgtttctggt
     1021 gtatggtcga gccgtttgaa tcgaaaaaag ggatcctatc tgttttgtca ggatacacgg
     1081 gcggacatgt tgaaaaacca aattatgatc aagtaagtgg tggttacaca ggtcatgtgg
     1141 aagctgtgga gatcatttat gacacaagag aaataagtta tagcgagttg ctgacaatct
     1201 attggcaaat tactgatccc acagatacat ttggacaatt tcaagataga gggaagcaat
     1261 atcgtccagt gattttttat caagtcgaac gacaaaaaga attagcggaa caatcaaaac
     1321 agaaactaga cagttcagga actttccacc aaccaatcgt cacaaaaatc gaaccagccg
     1381 gtacgttttg gccagcagaa aactatcatc agcaatttta caaaaaacaa ccgaaacgtt
     1441 ataaaaaaat ccagcaagca cgtaatcaat ttctgatcta tcaacgagta aaaaacaaat
     1501 ggcaaaaaaa tatacgaaaa aatcattttg attaactcct tttctttaaa atgtagtaaa
     1561 ctaaaaatag aaaaaagaaa gcgttttaaa aagatttgat gaggaggaga aaaattgtat
     1621 attgcagata gtgcacgtta tgaaaaaatg gaatatcgta aatccggtca ttcaggtttg
     1681 aaattaccga ttttatctct cggattatgg caaaattttg gtgattatga tcctatccat
     1741 aatcaaagag agattttaag aggcgcattc gacatgggaa ttacccattt cgaccttgcg
     1801 aataattatg gaggccctgc tggagctgca gagaaaaact ttgggcgtat ttttagagaa
     1861 gattttcaag cctaccgtga tgaactgatt atttctagca aagcaggtta tcatatgtgg
     1921 gaagggccat atggggaatg gggctctcgt aaatccatca tttcaagttg tgaccaaagt
     1981 ctccaaagaa tggggctaga ttatgttgac atcttctatc atcatcgccc agaccctgat
     2041 acacctttgg aagaaacagc agaagctttg atgcaacttg taagacaagg aaaagcatta
     2101 tatattggta tatccaacta taatggagaa gatacaaaaa aaatgactga aattttaaaa
     2161 agaaaggaag caccgttcat tattcatcag atgcgctaca atatgttttc tcgtgcacta
     2221 ctagaagacg atctatctcc agtattagaa gaagaagggc taggggcaat cacgttcagt
     2281 ccgcttgctc aagggttgct gacaaatcgt tatctacatg gtattcctga agattctaga
     2341 gcacatcgta aggaaatacc attcctttca gaagaacaag ttggatcaac tctggaaaaa
     2401 atcaaagcac tgcaaacaat cgccgtatca cgaggacaat cattagcgca aatggccctt
     2461 gcatggaatt tgcgacaaaa atctgttaca agtgtactgg tcggagcaag tcggttaagt
     2521 cagttacaag aaagtgttcg aatgatggat aatttagatt tctcaccaga agaagaattg
     2581 agaatcgacc aaattctaga atagaaacga tgtaatcaaa ctttttatcc atatattaaa
     2641 aaaaacaaaa gatgacatca agaatcgaat gaagatcgtc tgttattgaa catcatattt
     2701 tttgtcgttc aaaataaaaa tgatcgaagg ctaggagtga attttatcct agtctttttt
     2761 atggtaaata tcttcttcgt ttgtatgtct atgaacggat taatcaattt tgtatacaaa
     2821 aaatcatcag atgattcaaa cgtgttataa taattacgaa aaaatacatc agatgaaaag
     2881 gaggtatgtg gtttggaaga aaataaaacc ctagtagaag taactactga caagatcctt
     2941 agatatttaa aggaaatgca gttgcaagta ggagataaac tgccgaatga atatgaattg
     3001 tccgattatt tagaggtggg gcgaagcacg gtgcgagaag ctgtgcgggc tctagcttcc
     3061 aggaatatcc ttgaagttcg gcaaggagcg gggacatttg tcagtgataa gcgagggatt
     3121 gccaatgacc cactcggttt ttctttgatt gaagatcagg atcggatgat acgagactta
     3181 tttgaactga gatatttatt ggagcctaag atggcaggtt tggcagcgga atatgcaacg
     3241 aagaatcaaa tcgtccaaat gaaaaaatta acgcaaaaga ttgaaacttc ttttcgagaa
     3301 ggcacacaag aacatgttca attagacatc gattttcata cgatgattgc agaagctagc
     3361 ggaaacgtag ccttgctgca tattatccca atcattaatg aatcaattgc tttgtttaat
     3421 aaaaattatg atatccagca tctgaaaatc gagacgatcg atattcatac agagatcgtt
     3481 caggccatcg aaaggcggaa tgcacttgct gcgtttgatg caatgactgt ccatatggca
     3541 ataaatcgga gagaattaca gaaaataata ggagaaaaag aaactaattg acagttcatc
     3601 tgatgtattt tatgataaat acatcagatg aatttttgtt ttatatttgg taaaaaagag
     3661 gagtaaatag tttctgttca tcgaaatgta agcgctgact aatagacaaa aagagatgac
     3721 gtcaaggaga agaagcagga atgaggggat ctggaatgag ttttatggat aaattagcca
     3781 atgttttagg gaacgtggca acgagaatca ataatttaag gtatatcatg gtgataaaaa
     3841 acgcatttgc tgcattgatt ccggtaatta ttacgggtgc ttttggcaca ttgttttcag
     3901 caatggtctt tgactcggaa aatggcttag cacagataag tttcttgcgt tttttagaag
     3961 aattaaagcc tatcgcatct gccatcagct atgtcacact aagtttcttg accatctatg
     4021 ctgtttttct gatagggatc gaattagcca agctgaataa agtggatggt gtattccctg
     4081 gtatcatcgc agtgatgtct tatttatctg ttaatccaac tgtttatgaa ttagtgaatg
     4141 aaaatcagac tgttatagta gaaaacgtat tggctaaaca atatacagat acgaaagggt
     4201 tatttttggg aatgttggtc tccatccttt ctattgaact ttattgctgg ttgaggaaac
     4261 agaagaaact acagctgaga atgcctgata ccgtgccaac gaatgtttct gctagttttt
     4321 ctgctttatt cccgacgatt ctgactgtat cgggtatcgc ggctgcggga tttatcatca
     4381 aagaacttac aggaatgtac ctttatgata tcatttacaa tgtcgtacaa cgcccattag
     4441 aaggtgtcgt acaagggtta ccgggtattt tgctattgat gtttattgcg caagtctttt
     4501 gggtgattgg tatccatgga aatcaaatgg tgaagccgat acgggaaccg ttgcttttag
     4561 cggcaattgc tgtaaatacg gaagcttttg aagctggaaa agaagtaccg aacattatta
     4621 ctatgccatt ttgggatatg tatatgagta tgggcgggtc tggagtcacg atcggtttac
     4681 tgattgcagt attgttagtt ggaaaacggg aagatatgaa acaaatcacc aagttatccc
     4741 ttgctcctgg gatcttcaac atcaatgaac cagttatttt tggtatgccg atcatgctga
     4801 atccaatttt agctattccg tttatcttaa cgcctcttat cactggaacg attggttatt
     4861 ttgcaaccgc tttaggattt gcggcgaaag cagttgtaat ggtcccttgg ccaatgccgc
     4921 cgatcgtcaa tgcttatctg gcaacagcag gaaatatcgg tgcagttatt acgcaacttg
     4981 tttgtattat tgtttctatt ttgatctatc tgccatttgt aaaagtagca aatcgtgtgg
     5041 aaaatgatgg gatctaaaaa acaggaggaa atcatgaaaa ttactattcc aaaaaatttt
     5101 attcttggtg ctgcatcttc tgcttggcaa acggaaggtt ggaaaggaaa aaaagaagga
     5161 caggattcgt ttatagatag ctggtataaa aatgaacatt ttgtttggca taatgggtat
     5221 ggcccagccg tagcaactaa ttttatggaa caataccagc aggatgtgga tctgatgaaa
     5281 tccatccatc tgacacatta tcggacttct atcaattggg ctcgtttttt tactgattat
     5341 gagaactgta tagtagatga agaatatgct cagcatataa gtgaaatgat cgactctttg
     5401 atcgaagcag acgtagaacc gatgatttgt ttggaacatt atgaagtccc agcttttctt
     5461 ttggaaaaat atgatggctg gtcttcaaga aaagtagttg atttttatgc taagtatgct
     5521 caaattgcct ttgatcgtta cggtgatagg gtaaaaaact ggtttacatt caacgaacca
     5581 atcgttcccc aaacgcgttg ctatttggat gctgttcgct ggccgcatga acaaaacaca
     5641 aaaaagtgga tgatgtggaa ttatcataaa gttttagcaa cggctaaagc agtagagatt
     5701 taccgcagcg gaaattatgg tggaaggatt ggctgtattt taaatcctga aatggtctat
     5761 gctcgatcca attctccaga agaccaaaaa gctgcttata tgtatgattt gttttataat
     5821 cgagtatttt ttgatccgat ggtaaaagga tactacacag aagagttcct ggagttgtgt
     5881 caaaaacaat cgatttattt cgatcctgca acagaggatt tagaaacgat tcagcatcat
     5941 acagtagatt tccttggaat caatcaatat tatcctaaac gcgttcgcgc ccctcgttat
     6001 caatggaatg aagagacacc tttccatcct gaacgatatt atgaagtctt tgatttacct
     6061 gggaagaaaa tgaataattc caggggatgg gaaatctatc cgaaaatcat gtatgatata
     6121 gccatgtatc taaaagaaaa ttatcataat atcccttggc tgataacaga aaacggtatg
     6181 ggaagagaat atgaagaaca gtatatggat gagaaaggaa tcgtgcaaga tgattaccgt
     6241 atcgaattta tcggacagca tttgcattac ttgatcaaag caacagaaga aggcgcaaat
     6301 tgcgaaggct atatgttatg ggcattcact gactgtgtgt caccaatgaa tgcatttaag
     6361 aatcgatatg gattagttcg aatcgaattg aacgatgaac gaagcagaac attgaaaaaa
     6421 tctgcttcgt ggtatcagac tttgattgat aagagagaat tggatttgcc tgacgaaatc
     6481 tataaataaa tagaagtttt ttgtagatgt tcattgtatt tactggttat ttgaaataaa
     6541 agaggttgtg aatccagcgt tttgagctaa tacccagggt cagctgattg aacaccgttt
     6601 attgagaggt tgtgaaaaag ctgtcctgca tcaagtaata agaatagcaa agtaaaaaca
     6661 ac
//
LOCUS       DAYYME010000132         6523 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000132 DAYYME010000000
VERSION     DAYYME010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.133"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..926
                     /locus_tag="ACHBFW_002394"
     CDS             <1..926
                     /locus_tag="ACHBFW_002394"
                     /inference="COORDINATES: protein motif:HMM:NF016640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase domain-containing protein"
                     /protein_id="HFM9318662.1"
                     /translation="LYSLAINELEKRNLEIHELNQKNNSILGMKIRNKINQLNQMKKR
                     DNLLPAKYTLKSMTGKGPIKDYSNRENRSDELRKKELTNQGTPKRIAVYSCITGNYDS
                     IKEPDFSHPLVDYYLYTDNVITSEHWNIREIPKYISELKNNILINRYIKFHPNELFTD
                     YDFTLYVDGNVTITSDITGLLNSSSKLGLSMHKHSERKSLYSEADALIAVGKGNKESI
                     IEQISKYREEGFPDNFGLAEATVILVDLNNSIAENLLDLWWREYLNSESGRDQLAFPY
                     VLWKSGHAMKEVTTLGNNVYKSAKLRIISHY"
     gene            1178..2188
                     /locus_tag="ACHBFW_002395"
     CDS             1178..2188
                     /locus_tag="ACHBFW_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HFM9318663.1"
                     /translation="MKILITGGAGFIGFHLSRKLLKETENQIVAIDNLNDYYDVQLKE
                     DRLTILYDYNNFKFIRMDLLEKEKLDSLFESEGFDIVIHLAAQAGVRYSIENPYAYID
                     SNIIGFLNILEACRNFPVKHLLYASSSSVYGGNTKVPFSTEDSVDHPVSLYAATKKSN
                     ELMAHTYSHLYKIPTTGLRFFTVYGPFGRPDMAYFSFTKNILSGKEIKIFNHGKMERD
                     FTYIDDIVEGITRLINKVPEPNSDWTDIDDLSSESWAPYKIYNIGNNKPVALLEFVET
                     IEKYLGVKANKIFLDMQPGDVVRTYADIEDLEEKINFKPNITLDEGIKKFVEWYTVYY
                     LS"
     gene            2556..3980
                     /locus_tag="ACHBFW_002396"
     CDS             2556..3980
                     /locus_tag="ACHBFW_002396"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFM9318664.1"
                     /translation="MDSSSNKSHSIAKKAFIYAIGTLSSKMISVIIIPIYAVYISVAD
                     LGSFDYQNTIAQFLSPVIFGAIWEAILRMGLGKEKNIQKKVVTNSICFVIANTVILAI
                     FLPIIYRMIFPNLEYYQLFVFQLIFTPILSVFQYIVRALGESKIFVMSGIIAAGINLL
                     LLTIFVVTMGMGILGLLLSIIISNVINLIFLIFRGQIFQYFATSSFDFLFLKSMLKYS
                     IPLTLNLTFGWLIVGFSRFYIGTYIGVTENGIFSFAMKFSSIILTLTSIVSMSMIEDA
                     VITSENDDFIERFVEKNTYIFKMYIRLATLSIPIICCAYSFITNPDYKKSLVLVPILV
                     IMACFSGMTTNIGNIFAVYNKTNQLLMISVVSGLLSVVGTYLLGYSFGVIGVSFATLL
                     GTLVLFGLRYYFGRKIAYYAFDWKRISLLLVLYSAIAFVCIHRGFYFNLFLTIIFSAI
                     FVFTYKQLIITYLKKFFRIKMAKR"
     gene            4066..4494
                     /locus_tag="ACHBFW_002397"
     CDS             4066..4494
                     /locus_tag="ACHBFW_002397"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFM9318665.1"
                     /translation="MIFGKKHSLVEVPEFGSEPYLIEIGDNFRCSFGVKFITHDGGVW
                     VLRKMSSVSESTSKIGRIKLGNNIFIGIYSIVFPGVSIGDNVIIRANTLVNKDIPNNS
                     VVAGSSCKVISTIEEHYEKNKNEFIKYRTSMILKQKNFFY"
     gene            4582..5748
                     /locus_tag="ACHBFW_002398"
     CDS             4582..5748
                     /locus_tag="ACHBFW_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFM9318666.1"
                     /translation="MKISVFGLGYVGLANSLLLAQKEQVKAYDIVEEKITMLQNGISP
                     IEDKEVHEFLKRDDLNVEFTSNFTDAVNFGDYLIIATPTDYDEKKNYFNTSTVEDVIE
                     KALAIRPDSTFIIKSTVPVGYANEIKAKFGTENIIFSPEFLREGKALYDNLYPSRIIV
                     GECSARGREIATMFQRNAFNKDASVLLTHSTEAEAIKLFSNTYLALRVAYFNELDTYA
                     EFRGLDSKQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLRANYEKVPSNIISAI
                     VDANTTRKDFIAGQILAKKPNVVGIYRLTMKAGSDNFRYSSIQGIMKRIKAKGIEVVV
                     YEPTLDEDEFYHSQVLKDINEFKNISDVIVSNRFEDILKDVEEKVYTRDIFGDN"
BASE COUNT         2259 a          861 c         1205 g         2198 t
ORIGIN      
        1 aattatatag tttagctatc aatgaattgg aaaaaagaaa tcttgaaata catgaattaa
       61 atcaaaaaaa taattctatt ttaggcatga aaatacgaaa taaaattaat caacttaacc
      121 aaatgaaaaa acgagataat ctgttgccag caaaatatac ccttaaatcg atgaccggta
      181 aggggcctat taaagactat tcaaacagag agaatcgcag tgatgaacta agaaaaaaag
      241 agttaactaa tcaaggaaca cctaaaagga ttgctgtgta tagttgtatc actggtaatt
      301 atgattctat taaagaaccg gatttttctc atccattggt agattactat ttgtatacag
      361 ataatgtgat tacaagtgag cattggaata ttagagagat acctaaatat atatcggaat
      421 taaaaaataa tattttgatc aatagatata ttaaatttca tcctaatgag ctatttactg
      481 actacgattt tactttgtat gttgatggta atgtaacaat aacttctgat attaccggat
      541 tactgaacag ttcttccaaa cttgggttaa gtatgcataa acatagtgaa agaaaatctt
      601 tatattctga ggcagatgca ttaattgcag ttggcaaagg taataaggaa agtattatag
      661 agcaaattag taagtacaga gaagaaggat ttcctgataa ttttggattg gctgaagcca
      721 cggttatttt agttgattta aataattcaa tcgctgagaa tcttctagat ttgtggtgga
      781 gagagtattt gaactcagaa agtgggcgag atcagctcgc atttccttac gtgttgtgga
      841 agtccggaca tgcaatgaag gaagtgacta ccttaggcaa taatgtatat aaaagcgcta
      901 aattaagaat cataagtcat tattagccct aaatgatttc taaactattc tttcaaatcc
      961 tagtaaatat ttggagatag aagatttcct ttttaaagtc ataaaaaatt aagttatatt
     1021 ttttgagtga atggagaaga cgcatagtat cataaacttc gttatatctt aaattatggg
     1081 gtatgcctaa ataataaatg taatacgagt ataaagaaga aagcaatttt gggtttaacc
     1141 ctattggttc ctttcagaaa aaggtggatg aataaaaatg aaaatattaa taacaggtgg
     1201 agcaggattt attggttttc atttgtctag aaaattactc aaggaaactg aaaatcaaat
     1261 tgtagcgatt gataatctca atgattatta tgatgtccaa ttaaaagaag atcgattaac
     1321 gatactgtac gattataata attttaaatt tatcagaatg gatctattag agaaagaaaa
     1381 attagattct ctatttgaaa gtgaaggttt tgacatagta attcatttag ctgctcaagc
     1441 aggtgttaga tattctattg aaaaccctta tgcttatatt gattctaata ttatcggttt
     1501 tcttaatatt cttgaagcct gccgaaactt ccctgtaaag catctattat atgcatcatc
     1561 tagttcagta tatggtggga acacgaaagt accgttttca acagaagatt cggtggatca
     1621 tccagttagc ctttatgcgg ctaccaaaaa atcgaatgaa ttaatggctc atacttatag
     1681 ccacctttat aaaataccaa caactgggct gagatttttc actgtctatg gtccatttgg
     1741 gcgaccagat atggcctatt tctcttttac caagaatatt ttatctggaa aagaaattaa
     1801 gatttttaac catggaaaga tggaaagaga tttcacttac atcgatgata tcgtagaggg
     1861 aatcactaga ttaatcaata aagtacctga acccaactcc gactggacag acatcgatga
     1921 tttgagtagt gaaagttggg caccatataa gatatacaat attggaaata ataagccggt
     1981 tgcattgttg gagtttgttg aaacaattga aaagtattta ggtgttaaag cgaacaagat
     2041 ttttttggac atgcagccgg gtgatgtcgt tagaacttat gccgatattg aagatttaga
     2101 ggaaaaaatc aactttaaac cgaatataac cttggatgag ggcattaaaa aattcgttga
     2161 atggtataca gtgtattatt tgagttaatt ttgaaaggaa tattgaagat gggtaattca
     2221 tacgattttt ttagctttgt taggtatttt aggaaattct actttcaaac gtgacgaact
     2281 ttctcgccga atagatattt tctataacta aaagaatttc tataaaacaa aattgtgtat
     2341 caagatgtgt gaactggatt ctgactgcca atattgattg atgagattga agaaatatat
     2401 agttgatcat ggatatcttt atttgaaaag atcttagcta ttcacatatt caaaaaaatt
     2461 tttactacga ggactcacac gatacagagg aatttaggta gttctaatta aaccagaaag
     2521 tcatctatag atgtgagttg agggaggtca tacggttgga tagtagcagt aataaatcac
     2581 attcaatagc taaaaaagct tttatttacg caataggaac attatcatca aaaatgatat
     2641 ctgtcattat aatccctatc tatgcagtat atatttctgt tgccgattta ggaagctttg
     2701 attatcaaaa tacaattgct caatttcttt ctccagttat ttttggagca atatgggaag
     2761 caattttacg catgggatta ggcaaagaaa aaaatattca gaaaaaagta gtgacgaaca
     2821 gtatttgttt tgttatagca aatacagtga tattagccat ttttttacca ataatatata
     2881 gaatgatttt cccgaattta gagtattatc aattgtttgt ttttcaatta attttcactc
     2941 ctatcctaag tgtttttcaa tatattgtaa gagcactagg tgaatcaaag atttttgtga
     3001 tgagtggtat aatcgctgcg ggtatcaacc tgttactcct tactattttt gttgttacaa
     3061 tgggtatggg gatacttggt ctcttactct ctatcataat ttctaatgtt ataaatttga
     3121 tttttttgat tttcaggggg cagatctttc aatatttcgc aacgagttct tttgattttt
     3181 tatttttaaa gtccatgctg aaatactcta tcccactcac tttgaatctc acttttggat
     3241 ggctaatagt tggtttttca agattttata ttggaacgta catcggagta acagaaaatg
     3301 gtattttttc ttttgcgatg aaattttcat ctataatttt aactcttaca agtattgtta
     3361 gtatgtctat gatagaggat gcggttatca cgtctgagaa tgatgatttc atcgaaagat
     3421 tcgttgagaa aaatacctat atttttaaaa tgtacattag gttagcgact ctttctattc
     3481 ctatcatttg ctgtgcatac tcttttataa ccaaccctga ttataaaaaa tcattagttc
     3541 tagttccaat attagttatt atggcatgtt tcagtgggat gacaacaaat atcggaaata
     3601 tatttgcagt atacaataaa acaaatcagt tattaatgat ttcagtagtg tcaggtctat
     3661 tgagtgttgt tggcacctat ttattaggct attcttttgg agtaattggt gtctcatttg
     3721 ctactttgct cggtacttta gtattatttg gacttagata ttattttgga aggaaaattg
     3781 cttattatgc atttgattgg aagcgaatat cgctactttt agttctctat tccgcaatcg
     3841 cttttgtatg cattcataga ggcttctatt ttaatctttt cttaacgatt atttttagtg
     3901 ctattttcgt ttttacgtat aaacaattaa ttataacgta tctaaaaaaa tttttcagaa
     3961 taaaaatggc taaaaggtga gggtatacga agtaagaata gaatctttcg aagaataaag
     4021 agaaaactat ttggaaaaaa tctgcgagaa tgtattaatg aaggtttgat attcggaaaa
     4081 aaacatagtt tagtagaggt tcctgaattt gggagcgagc catatttaat tgaaataggg
     4141 gataatttca gatgttcatt tggtgttaaa tttataacac acgatggtgg agtctgggtc
     4201 ttaaggaaga tgagttctgt gagcgagtca acttcaaaaa taggtaggat caaattggga
     4261 aataatattt ttattggtat ttactctatt gttttcccag gagtttcaat tggcgataat
     4321 gtaattatta gagcgaacac tttagtaaat aaagatatcc caaataatag tgttgtagcg
     4381 ggtagttcat gtaaagtgat ttcaactata gaggagcatt atgagaaaaa taaaaatgaa
     4441 tttattaaat atcgaacctc gatgattttg aagcaaaaaa attttttcta ttaaataata
     4501 ttgattgaat aaggaatatg ttcaaaagga taacagctaa aatactataa aatgaaattt
     4561 gattggagcg tatggaagaa tatgaaaatt tcagttttcg gattaggcta tgttggtcta
     4621 gcaaattcac tattattagc acaaaaagag caggtcaaag cttatgatat cgtggaagaa
     4681 aaaatcacga tgttacaaaa tggcatctca ccaatagaag ataaagaagt tcatgaattt
     4741 ttaaaacggg atgacttaaa tgtcgaattt acgtcaaatt ttactgatgc agtaaacttc
     4801 ggtgattact tgattattgc aactccaaca gattatgacg aaaagaaaaa ctattttaat
     4861 acatcaacag tagaagatgt aattgagaag gctttagcaa ttcgtccaga ttcaacattc
     4921 attattaagt cgacagttcc agttggctat gcaaatgaaa ttaaagcgaa atttggtaca
     4981 gaaaacatca ttttttcacc tgaatttttg cgtgaaggaa aagctttgta cgacaactta
     5041 tatccatcaa gaattatagt tggagaatgt tctgctcgtg gtcgcgagat tgcaactatg
     5101 tttcaaagaa acgcatttaa caaagatgca tcagttcttt tgactcactc aactgaagca
     5161 gaagctatta aattattctc gaatacatat ttggcattgc gagtggcgta tttcaatgaa
     5221 ttagatacgt atgcagaatt tcgtgggctt gatagtaagc aaattattga aggtgtcggt
     5281 ttggatccac ggatcggttc gcattataat aatccttctt ttggctatgg tggatactgt
     5341 ttacccaaag acacaaaaca attaagagcc aactatgaaa aagtaccaag caatatcatc
     5401 agtgcaattg ttgatgcaaa tacaacaagg aaagatttta ttgctggaca gattttggcg
     5461 aaaaaaccaa atgttgttgg aatctatcgt ttaacgatga aggccggttc agataatttt
     5521 cgatattcgt cgatccaagg aattatgaaa cggattaaag ccaaaggaat tgaagtggtt
     5581 gtttacgagc ctacattgga tgaagatgaa ttttatcatt cacaagtatt gaaagatatt
     5641 aatgaattta aaaatatttc tgatgtgatc gtatctaatc ggtttgaaga tattttaaag
     5701 gatgttgaag aaaaagttta taccagagat atctttggtg ataactaaat aataatgaac
     5761 gggtacatgg aggaaaatat tttagatggt gactcgaaat aaatgtttgt tctcattgca
     5821 tctgtatcta taccgataag tttaaaaatt ccgattagga aaatgaagtt ggctatcctt
     5881 gcgaggaatt tattgctgga ttattttatg aactaaagca agacatcatc agaaaaaaat
     5941 ccaaaatttg acaatgggat tgatcgttta ccagctgtaa gcgaagtatc tgacacgaaa
     6001 atggaaaatg caattactgt tattaccaat agcttttttt catcgatcaa gtatttgaaa
     6061 acattatttg tttgccaagc tgatcaatgt tctttcagac ttggaagaac attgatcagc
     6121 tcacaagtaa tgattgaagt ttaggttatg ggataaagga taagtgaaat aaaagtcgtg
     6181 tctgcatctt tgttagtacc agtaaatttt tctactacgg cgctagtcac gacagtccag
     6241 gcatctgaaa ttgaagatgc aaatagagtt tctgctagtt taggtattga tgttactgta
     6301 ttagcattaa tcttagagcc agtagcatcc aataggtgta gctatagttc taatagtata
     6361 aaacaatgca gaggatatga ttgctttatg tctcggtgtg tcaggtcaaa atagagttcc
     6421 atatggggcg atttctcaat gaataatctt atagatataa tcttgtttgc tatttcgtta
     6481 tcacttttac ttttcttcga ttatttcaaa aataaaaatc caa
//
LOCUS       DAYYME010000133         6479 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000133 DAYYME010000000
VERSION     DAYYME010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.134"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..759
                     /locus_tag="ACHBFW_002399"
     CDS             22..759
                     /locus_tag="ACHBFW_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9318667.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            773..1588
                     /locus_tag="ACHBFW_002400"
     CDS             773..1588
                     /locus_tag="ACHBFW_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM9318668.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1601..2248
                     /locus_tag="ACHBFW_002401"
     CDS             1601..2248
                     /locus_tag="ACHBFW_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9318669.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2264..2920
                     /locus_tag="ACHBFW_002402"
     CDS             2264..2920
                     /locus_tag="ACHBFW_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9318670.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3082..3903
                     /gene="racE"
                     /locus_tag="ACHBFW_002403"
     CDS             3082..3903
                     /gene="racE"
                     /locus_tag="ACHBFW_002403"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFM9318671.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3906..5261
                     /gene="rph"
                     /locus_tag="ACHBFW_002404"
     CDS             3906..5261
                     /gene="rph"
                     /locus_tag="ACHBFW_002404"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFM9318672.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5262..5780
                     /locus_tag="ACHBFW_002405"
     CDS             5262..5780
                     /locus_tag="ACHBFW_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9318673.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5887..6387
                     /gene="cbpB"
                     /locus_tag="ACHBFW_002406"
     CDS             5887..6387
                     /gene="cbpB"
                     /locus_tag="ACHBFW_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFM9318674.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         2126 a         1090 c         1436 g         1827 t
ORIGIN      
        1 taataatggg agtgtaattc tatggcaatg attaaatttg atcacgtgga taaatattat
       61 ggcaagttcc acgcattaaa aaatatcaat ctcgaattcg agaaaggaga agttgtcgtt
      121 gtcattggtc cttctggttc aggaaagagc acgatgcttc gctgtatcaa tggattagag
      181 acgatctctt caggtaagct gctgattaat gatactgact tgcatgataa aaagacaaaa
      241 ctgacagaag tccgaaaaaa catcggcatg gttttccaac atttcaattt gtacccaaat
      301 aaaactgttc tggaaaacat caaattagca ccaatcaaag tgttgaagca agaccaggca
      361 actgcagtca aaaacgcgga gaaatttttg aaaactgtca atatgttaga taagaaagat
      421 tcttatcctt ccatgttatc cgggggacaa cagcaacggg tggcaatcgc cagaggttta
      481 gcgatgaatc ctgagatgct gctgtttgat gaacctactt ctgctttgga tccggaaatg
      541 attggtgatg tattagacgt tatgaaaaaa ttggctcatg acggtatgtc aatgattgtc
      601 gtgacacatg aaatgggctt tgccaaagaa gtggcggatc gtgttatctt catggctgat
      661 ggagaagtat tggaagacag cagaaatgtg agagactttt tcgaaaatcc aaatgaagtc
      721 cgagcacagc aatttatcag taaagttatc aatcattgat aggaggaaaa agatgcgcag
      781 gttaaaaaaa atacgcctga ttttcctttt aggcattgct tttcttgcac tagccggatg
      841 taaaggaaat gggttggcaa atgaagatat tttagagcga agcaaatcta ccaatgaaat
      901 catctggggt gtcaagtatg atactcgatt atttggaatg atggatattg agagcagaac
      961 tgtacaagga tttgatgtag atattgcaaa agcaatcact aaaaagatcc ttggtgacaa
     1021 tggaaaaaca gaatttgttg aagtcacttc taaaactcgg attccattat tgaaaaacgg
     1081 aaatatcgat gcaattattg caacgatgac gatcacagat gaacgaaaaa aacaggttga
     1141 cttttcagat gtctatttcg atgctggcca agctttgttg gtaaagaaag gaagccaaat
     1201 caaaagtgta gatgatttga atgcttctac aacggttcta gcagtcaaag gttctacatc
     1261 ggctgcgaat atccgacagc atgcaccaga tgcaaaaatt ttagaattag aaaactatgc
     1321 agaagcattt actgctttac aatctggtca gggagacgcg atgactacgg ataatgcgat
     1381 tttgttaggg attgcagatg aaaatccaga gtatgagtta gttggtggaa cgtttactaa
     1441 tgagccttac gggattgcga tcaacaaagg gcaagaaaac ttcttgaaag ctgttaacca
     1501 agcgctagag gaaatgcatg cagatggcac atacgataaa atctatcaaa aatggtttcc
     1561 aaatgaaact gaaggaaaag tagagtaagg gggagaggca atgagcgaat tgatacaaac
     1621 ttatggacca atctttgtcg acgggttcaa gatcacattg ttttcgagtg tcttagcact
     1681 tgtgtttagt ttgattatcg gaacgttaat ggcaatcttc caattatcaa agaataaagt
     1741 agtgagcgcc ttagcaaagg catatgtaga attttttaga aacattccat tattaatcat
     1801 cgtgatgttt ttttatgttg tcgtaccgat ttattggttt aatctagatg gattccaagc
     1861 aggtacgatt ggattaacga tctatacatc tgcttttatt gctgaaacag tacgttcagg
     1921 gattcaaacc gtaccaaaag gtcaaacaga agcaggacta tcttctggtt ttacctattc
     1981 agaaatcatg cgttatattg ttttaccgca agcattcaaa atcgttatcc cgccattagg
     2041 caaccaattt atcaacctcg taaaaaactc atctatttta gcaatcgtag ctggtttaga
     2101 tttgatgtat caaggagact tgatcgctag tgaaacattc aataccttcg atacgtatat
     2161 catcgttggg ttattttact tagttatcac gctaccgtta tcttacttaa tggtttatct
     2221 tgagaaaaaa tgggcggttc gttcatagaa ggaggggaaa tacatggatt ttagcggcgc
     2281 tttttcatgg atgaatatcc gttttttatt ggaaggatta aaggttactg ttgaagtttc
     2341 attgttttcg atcattttta gtttcttgat cggtggtctg acaggtgtga ttcgttttgc
     2401 gaatatccct ttcttttcca aaatccttgg tgtagtgatt gatattatac ggaatcttcc
     2461 attattattg atcattttct tcacttactt tgccttacct caaatcggca ttcagatgaa
     2521 catcttctgg tccgctgtcg cagcattaac gatttttgag tcagctatgt tgtcggaaat
     2581 tttccgagct ggactcaatg ctgttccaaa aggacagatg gaagcaggat tatcaacggg
     2641 actgacttat gtagaatcga tgcgaacgat cgtgttaccg caagcattta aatcaatgat
     2701 cccggcaatt gtgagccaat tgatttcact gatcaaagat acttcattag cagtaatcat
     2761 ttctttacct gaactttctc atcaggcacg tattatttat ggacagaaca ccaattatgt
     2821 gcttccgatg ttcatcatca tgacacttat gtacttcgtg gtatgttacg cgctttctct
     2881 gctttccggt tacttggaaa agagacggta cagttattaa tagatgattc ttttagcggt
     2941 tgtggcaaac taaagtcaca accgcttttc ctctttccat tcgaaaaaaa gagatttcga
     3001 ttgttttaag caagtaagag cacttgaaaa ttcacgctaa aaagtacaca atagggagga
     3061 tagaatttat tgaaaaaaag gatgatacga ttgacagata atcgccctat cggatttatt
     3121 gattcaggtg tcggcggctt gactgtagta aaagaagccc tgaaacaatt accgaatgaa
     3181 aatattttat ttgtaggaga cacagcacgc tgcccatatg gccctagacc cgcggaacag
     3241 gtaatccagt atacttggga aatgacggat tatctggtgg agcaaggaat caagatgctg
     3301 gtgatcgcct gcaataccgc aactgcggtg gctttagaag aaatcaaagc tgctctttct
     3361 attccagtca tcggtgtgat ccttcccggt actagagcgg cagtaaaaaa aacacaaaat
     3421 aaacaagttg gcattatcgg tacgattggt acggtaaaaa gtcaagctta tgaaaaagca
     3481 ctgaaagaga aagtaccaga attgactgtg acaagtcttg cttgtcccaa atttgtttca
     3541 gttgtcgaaa gtaatgaata ccattcatcg gtggcgaaaa aaattgtggc agaaacatta
     3601 gctcctttaa ccactaaaaa aatcgataca ttgattttgg gatgcaccca ttatccatta
     3661 ttacgcccca tcattcaaaa tgtaatggga gaaaatgttc aactgatcga ttctggagca
     3721 gaaacagtag gtgaagtatc tatgctgtta gattatttca atttgagcaa ttcaccgcaa
     3781 aatggtcgga cattatgcca gttttataca actggctctg ccaaactttt cgaggaaata
     3841 gctgaagact ggcttggaat cggacactta aatgtagaac atatcgaatt gggaggaaaa
     3901 taaggatgcg tcatgacggg agaaaagccc aagagcttcg caaactggag cttcaaacaa
     3961 atgttttcaa atatccagaa ggatcggtag tgatccgttt tggtgataca acagttattt
     4021 gttctgcaac aatagaaaat tctgttcccc cttttttgag agaaacagga acaggatggg
     4081 tgacagcaga atatagtatg ctgcctcgag caacgagtac gagaaatcgt agagaaagca
     4141 gcaaagggaa actttctggt agaacaatgg aaatccagcg tcttattggt cgttccttgc
     4201 gtgcagtagt agacttggaa aaattaggag aacgtagtat catcgtagac tgcgatgtga
     4261 tccaagcgga tggcggtact agaacagcaa gtatcactgg ggcatttgtg gcattgaaat
     4321 tagccattga aaaactttta agagaaaaag aattgtcaga agatccaata aaagaacatc
     4381 ttgctgcagt tagcgtagga atcttaccag atgggacttg cgtcacagat ttggattacc
     4441 aagaagattc cgcagcactt gtagacatga atcttgtaat gacagaatct ggaaaattcg
     4501 tggagattca aggaactgga gaagaagcaa cgtttgatgg tgaacagttg aatgaaatgc
     4561 tcttttttgg aaaaaacgcg attgaagatt tgatcaaaga gcaaaaacat gctttgctaa
     4621 cagagtttgc tcaaaatgat gagcggatag aagaaacaaa aacaatcatc attgcgacaa
     4681 gaaatccagg aaaagcagaa gagtttcgaa atatgttcaa agaagcggga tatcatgtaa
     4741 aaacattact tgactatcct gaattgccag atgtggaaga aactggcagc acatttgaag
     4801 aaaatgcccg attaaaagca gaaacgattg cacaattgct tgatcagcca gtcttagcag
     4861 atgattcagg tttgaaagtg gatgctttag gcggtatgcc aggaatctat tctgcacgtt
     4921 ttgcagggga acaaaaaagt gatgcgggga ataacgccaa attattatat gaattgacag
     4981 atgtgccgga tgaaaaaaga acagcacaat tccattgtac gttagtattc gcggcaccca
     5041 aaaaagacag ccttgtagta gaagcggaat ggcccggtcg agtagcccgt ataccatctg
     5101 gcgaaaatgg ctttgggtat gatcctttat ttatcccaga aggtaagaaa caaacagcag
     5161 cagaattatc gagcgaagaa aaaaacaaga tcagtcatcg ggctcaagca atgaaaaaat
     5221 tgagtgccga gtggaaacaa tggctggaag gagagagata gatgcggtat ttagtagtaa
     5281 gtgataatca tggagacaga gatatcctcg ttgacttagt agatcgttat cgttctgaag
     5341 tggatatctt tttccattgt ggtgattcgg aattagaagc ttctgaccca ttatgggata
     5401 cattccaagt ggttcaaggt aattgtgatt atggaccagg attcgaacag aaaaaagtga
     5461 tccaaactgg tcaggataca gtattcatga ctcacggtca tttatcgaat gtccgatttg
     5521 gtttgacaca gttagcaatc gaagctcgag ctgcaactgc cgatatggtg ttttttggtc
     5581 atacccatca aataggctgt gagatggatc agaaaatcct gtttttgaat cctggtagca
     5641 tttctcagcc tagaggcccg atccaaatcc catcatatgc tcttatcgaa agtttagatg
     5701 accaaatttc tgttcaatac tataaccgtg cccatcaggc agttgaagag ttggcctttt
     5761 ctttttcgag agagcgctga ataaaaaaac tctaattttt aataaaaata agctttattt
     5821 tatgaatttt gcatccggtt tcggtaaaat agtagtgttg aatgaaatag aaatggaggc
     5881 catcagatga ttggacctat agtaagagaa atgctgctgg aaaatcagga aacgtttttg
     5941 attcctgcag aaaatgtggc aaatgttatg tatcatcatc cattgtccca tggtttactt
     6001 gtgttatcga aggtcggtta ttccaaaatt cctgtattag gaaaagatga tcactttgta
     6061 ggactagtaa gtttatcaga tgtggtcaat aaaatgattg acttgcaaac aatcagcatg
     6121 gacccgttag aaggactaac ggttgctgat gtgacagaaa cagatgttcc gacaattgat
     6181 gaaaattggg aactagaaga agtactccat ttgttagtcg attcgccttt tttaccggtt
     6241 gtcacagaag atcggatttt caaaggaatc attacaagaa aagagctgct taaagccgta
     6301 aacttcatgg tgcatgaatt agaacgtcgt aatatattgt ctccgaaaat cgaagaatta
     6361 aaagaacaaa tcgatatcgt gagctaaaga acgagaaact aaaagataat caaaaaataa
     6421 gtagaaaaaa cagcgtacca gttttgactt tatcttgaaa aagaggctgg gacaaaagc
//
LOCUS       DAYYME010000134         6467 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000134 DAYYME010000000
VERSION     DAYYME010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.135"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="ACHBFW_002407"
     CDS             complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="ACHBFW_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFM9318675.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="ACHBFW_002408"
     CDS             complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="ACHBFW_002408"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFM9318676.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2298..2960)
                     /locus_tag="ACHBFW_002409"
     CDS             complement(2298..2960)
                     /locus_tag="ACHBFW_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9318677.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3144..4790
                     /locus_tag="ACHBFW_002410"
     CDS             3144..4790
                     /locus_tag="ACHBFW_002410"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM9318678.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4781..5863
                     /gene="uxuA"
                     /locus_tag="ACHBFW_002411"
     CDS             4781..5863
                     /gene="uxuA"
                     /locus_tag="ACHBFW_002411"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM9318679.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5971..>6467)
                     /locus_tag="ACHBFW_002412"
     CDS             complement(5971..>6467)
                     /locus_tag="ACHBFW_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFM9318680.1"
                     /translation="PIFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILEKENVQ
                     WMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQNDFMENC
                     NIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQSKKEN
                     QVTS"
BASE COUNT         2000 a         1214 c         1216 g         2037 t
ORIGIN      
        1 tcttgtcacc atcctttttt ccgctctctg actttttaca atcttcatag ctcaagtgcg
       61 catcagataa tcgaatgctc ctaaagctgc agtagctcct gaacccattg aaacaatgat
      121 ttgtttgtat ggactatctg tacaatcacc agctgcgaaa acgccgggga tattcgttgc
      181 gccatttttc tcggtcacta tttcatttct tggattcatt tcgatcgttc catctagcca
      241 atccgtatta gggagtaagc cgactagaat gaatactcct gaaacagcta gtgtatgcat
      301 ctccatcgta cggcgatcga tataggagag agattctacc tgattcgtac cgctgatttc
      361 tttcgtcgct gcatgtttga taacagtgac attcgcaaga gagactagtt tatcctgtaa
      421 gatttgatct gcttttaatt ctggtaagaa ttctagaaca tatacatgtt ttgctaaccc
      481 agccaaatcg attgctgctt caacaccaga attaccacca ccgataacag caatctcttt
      541 tcctttaaat aaagggccgt cacagtgtgg gcaataagca attcctttat ttttaaactc
      601 tttttcgcct ggtacattga tagaacgcca gtgcgcacct gtcgcgatga tggcggtttt
      661 tgctttaagt gatataccgt tagctaagcc aatattaata aaatgttgct ctttatttaa
      721 ggaaacggcc tgttgattca tcataatgtc gatagggtaa ctacgcacat gactttctac
      781 ttgctgcatc aattttggac cttctgtgta agaggtacca atcatatttt caatccctag
      841 tgtttcaacg acttgtccgc cgaaagaatc agcgacaagt cctgtacgga ttcctttacg
      901 tgctgcatag atagctgcac tactagctgc tggtccaccg ccaatcacta atacatcata
      961 aggttctttt tcaaacaact cttcttcaat caaagggcca gtgatttttt caacaatttg
     1021 ttcgatcgtc atgcgtccgc tactaaattc ttctccattt aaaaacacag ttggcactgc
     1081 catgattttt ttatcatcga tttctttttg gaacatccca ccttcaatca tcgtatgagt
     1141 aatatttgga tttaatacag ctaatatatt caatgcctgt acaacgtcgg ggcaattatg
     1201 acaagtcaag ctaacataac tgtcaaaatg caatgtttgt gttatttttt tgattttaag
     1261 actcaaagat tcactgattt tcggtgcccg tccactaatt tgtaacatag ccagtaaaaa
     1321 agaggaaaat tcatgaccga gggggatacc agcaaaaacg attccacttt ctttgccaac
     1381 tggattgata gaaaaactag gtgtgcgcat cgaatctctt ttttcccaag agattttagg
     1441 agacatttct gctatttcct tcgtaaattc agctagtttt tgactatttt cactattatc
     1501 caaactaaca gaaaaaacaa taggagattc aatcagtgct aaatattgtt cgagattttg
     1561 tttagtttct ttgtctaaca tatccaccac ctaaatcttt ccaacaagat caaaacttgg
     1621 tttcaatgtt tcgccacttt ctttccattt tgccggacaa acttcacctg ggtgggtacg
     1681 tacatattga gcagcgcgaa tcttatcaat cagtgcacta gcgtcacgac cgattccatc
     1741 tgcattgatt tccatagctt gaacgattcc gtctggatca ataataaatg ttcctcgctg
     1801 agctaatcct tgctcttcat ccaaaacatc aaaaatacga gaaatctgat gagacggatc
     1861 accgatcatc acgtattcga ttttaccgat agcatctgat gtatcatgcc atgctttgtg
     1921 agtaaagtgg gtatcggtag agcaagaaaa gacttcaaca cctaaagatt tcaatgtact
     1981 gtattgttct tgtaagtctt ctaattcagt aggacaaaca aaagtaaaat cagctggata
     2041 gaagcaaaga atactccagt tccccataag atctttttca ctgacttcta aaaattctcc
     2101 ttgatgataa gcatgtgcat taaattcttc gatttttttc ccaatcaatg acatagtaaa
     2161 acctccaata ataattattt atttataatt attgtaattt agttggagtt gcaaagtcaa
     2221 tggatatttt gttatttaat aaagattact caaaaacgaa ggttaattat aaaaatagaa
     2281 gaggtcgcaa gaaaatgcta ctgctggaaa tactcgggat aacgttgagt aacagtttcc
     2341 tgttctttca gcatcatgtg taagtgcaat cccgtttgaa atcgagcttc ttctggtttt
     2401 ttatcaacta aagccttgag taaatcataa tgttgttcca tgatcgtttc ccatggaagc
     2461 ccctgaatgg tcactcgtaa ccagcggaaa cgttctaaat gggtattatg gtcatctaac
     2521 cagttccaaa cttcttttct tcctgcaatt tcgaaacaag cttcatgaaa taaattatct
     2581 gcacggaaaa aagcgcgttc gtcatttatt ttgactgctt tttcttgttc ttctaaaatc
     2641 gtttctagca ctattcgtgt ttgtgtgtcc aatttggaac aacattcaat catgatttgc
     2701 tcttccaact gttctcggac aaagcgagca ttttctgcag aaacaagatt aattttggaa
     2761 acatacgtac cactttgagg tatcgtatac acgagttctt gctgacgtag ttgaatcaat
     2821 gcttcgcgaa tgggagtccg gccgatagca agatgttcct ccagtgcctt ttcagatatt
     2881 ttccttccag gttctaagtc agcatagata atttgtttac gaatcgcttg ataagcttgt
     2941 gattgcaagc tgcttttcat ctttattcct ccaaaatcat ttctatgaac gtagtataac
     3001 agaagaggtc tgaaaaacaa tctggtatgt cacattaact agtaaatttg tataaatagt
     3061 aaaattttgg ttgacatagc gatttttatt gcataaaatt cacttgaata caaaaactaa
     3121 tatgttagtt taggagggga aaaatgattg atttaaacaa ctggaggaac tttatccctg
     3181 aattatctca aaaagaagta gaaatacctc attttgaccg tgcatcatta agagaaaatg
     3241 gacaaaaaaa tcctgtctgg atccatttcg gtggcggaaa tctttatcga ggatttcatg
     3301 cagaaatcgc tcaacgttta gcggatctag gcgaattgtc cactggtgtc attgtttgtg
     3361 aaacattcga tgaagaaata attgaaaggg cttaccaaga ttttgatgat ttgattcttg
     3421 aagtggtcat gccaagcagt ggagagttcg tcaaacgtat attagctgcg acagctgaca
     3481 gtctgtattg tcatccagat tgttctgaac actttgataa gatgattcga cactttgaaa
     3541 atcctagttt acaattagtt acttttgcta ttactgaaaa aggttatcga ttgaaagata
     3601 caaataataa atatatagga ccgattgcag tcgatataaa aaatggtcca aagaagccat
     3661 cacatacaat gagtattctt gctgtgctac tttataaacg ttttttatct ggagcttggc
     3721 cgattgcact tgtctctaca gataattttt cacaaaacgg tagtcaatta cgtacttctt
     3781 tgctaacgat cgcaaaagaa tggcaaaagg aaggttatgt acaaaataca tttatccaat
     3841 acttatcgaa tgaagaaatt gttgcgtttc catggacgat gattgatcgt atcaccccga
     3901 atccttcctt gtcagttagc aaaatgttgg caaaagaggg cttttcaaat atgtcgattt
     3961 tccaaaccgc aaaaggaaat aatattgctc catttgtcaa tacagaggac atccattatt
     4021 taatcatgga agatcatttt cccaatggac gtccgaaatt cgaaaaagct ggggtattct
     4081 tgactgatcg tgcaacggta gataaagcag atacgatgaa ggtgacgact tgccttaatc
     4141 ctttgcatac gtctttagct attatgggtt gtttactggg gtataggagc atagctgagg
     4201 agatgaaaga tccagattta tcgctgctag tttctgaaat cgggtacaat gaaggcctcc
     4261 cagttgtgga gtctccaggt atcattgatc caaagatctt tctggaagaa gtaattactg
     4321 tgcgcttttc taatccaaat attcctaata tgcctcagcg aatcgccacg gatacctctc
     4381 aaaaggtggc gattcgttat ggtgagacga ttaaaaaata tatggaaata gaagttaaag
     4441 acccgcagac acttgtattc ataccattga ctctcgctgc ttggcttcga taccttattg
     4501 gcattgatga tcaaggaaaa ccattcatcc cgagtccaga cccactattg acagaccttc
     4561 agcataagct ttccgatatt caactgggta cgcaagatac atcacatatt catgagaaaa
     4621 tggctcctat tttgtcaaat aaagaaattt ttggtgtaga tctttaccaa gcagagcttg
     4681 gcagaaaaat agaagaaatg ttcatcaaga tggtagcagg accaaatgcc gtacgaaaaa
     4741 cattacagga ttacttacaa gaacatggag gaaatttatc atggaaatga cttttagatg
     4801 gtacggatca caggaagaaa agattacctt agatgaaatc agacagattc ctgcggtcaa
     4861 agggattgta ggcacattga tggatatacc agtaggagag gtatggccta gggagaggat
     4921 caaagaacta aaagaagaaa ttgagtctcg tgggttaaca ctaaaagtaa ttgaaagcgt
     4981 caatgtgcat gatgccatta aaattggatt accttcccgt gagcagtata tcgaaaacta
     5041 taagcagaca atccgaaact tggcagaata tggaatagaa gtcatctgct ataattttat
     5101 gcctgtattt gactgggtga aatctgacct tgactaccgc ttagaagacg gctcatccac
     5161 gttagctttt atatctgatg atattccaga tgatccaaga gaaatcgtcg cgcgcatcga
     5221 acattccagc aatgaatttg gattgccggg atgggaacct gagcgcttag ctcctatcaa
     5281 atccttgctt gaagcctatg catcagtaga cgaagaaaaa cttcgagaaa attttgccta
     5341 ttttctccaa tcaatcatcc caacatgtga agaggtaggg gtcaaaatgg ctgtacatcc
     5401 agatgatccg ccatactcga tttttggatt agctcgaatc gtaaagaatc gagaagattt
     5461 agattggttg tgtcgcatag tagacagccc ttataacgga atcactcttt gtacagggag
     5521 tattgcagaa gatccagaaa atgatgtata tgggatttta gcagaatttt gccaaagaga
     5581 tcgaatccca tttgcacatg ttcgaaatat taaattttta gctaatcgag atttctatga
     5641 ggcaccacat aaatccgaat acggttcatt agacatgtac cgcatactaa aaacaatgta
     5701 tgacaacgga tttaacggat acatccgtcc tgatcatgga agaatgatct ggaatgagac
     5761 aggaagacca ggatacggct tatatgatcg agcattaggt gctgtttatc taaatggatt
     5821 atgggaagca atcgaaaaag gaaacaaaag taaagataat tagtgaaaaa aactgtaatt
     5881 ttattatgta aataagaagg atgttcaaat tcttgataca taaatccgga ggttgttaat
     5941 aagcattcgg atttatgtat cttatttggt ctaggaagta acttgatttt cttttttcga
     6001 ttgatccatg agtgcttctt cttttaattc aaagatatat tctgagatcc tcataatcac
     6061 ttttttccag tcttctggtg tgatgattcc ttttaaaata tagatttttt tgatatgaga
     6121 aggcaattgt ttcaaagggc agtcgctgat aacgatgttg cagttctcca taaaatcatt
     6181 ttgtattttg atcgattcat ttgaaaaaat ttgttctatc ttaacgatca gatctttact
     6241 gatataaaaa tcacgcgtat agttcacacc aatcacaatt cgcggctgtt ttttgtagcg
     6301 atcatacaaa tgtactaggt cttcaaccat ccattgcaca ttttcctttt ctaaaataac
     6361 tggtaacaag tgttgatttt gttctctgaa atccatataa aattgttcaa tggcggtttg
     6421 gacttctttc aatacgctat gttcttgata aagcatgaaa ataggaa
//
LOCUS       DAYYME010000135         6388 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000135 DAYYME010000000
VERSION     DAYYME010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.136"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..338)
                     /locus_tag="ACHBFW_002413"
     CDS             complement(<1..338)
                     /locus_tag="ACHBFW_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318681.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEF"
     gene            complement(546..1472)
                     /gene="glsA"
                     /locus_tag="ACHBFW_002414"
     CDS             complement(546..1472)
                     /gene="glsA"
                     /locus_tag="ACHBFW_002414"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFM9318682.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1495..2922)
                     /locus_tag="ACHBFW_002415"
     CDS             complement(1495..2922)
                     /locus_tag="ACHBFW_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9318683.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2953..4167)
                     /locus_tag="ACHBFW_002416"
     CDS             complement(2953..4167)
                     /locus_tag="ACHBFW_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFM9318684.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(4253..4480)
                     /locus_tag="ACHBFW_002417"
     CDS             complement(4253..4480)
                     /locus_tag="ACHBFW_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318685.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            complement(4508..4750)
                     /locus_tag="ACHBFW_002418"
     CDS             complement(4508..4750)
                     /locus_tag="ACHBFW_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9318686.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(4782..5339)
                     /locus_tag="ACHBFW_002419"
     CDS             complement(4782..5339)
                     /locus_tag="ACHBFW_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9318687.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(5352..5540)
                     /locus_tag="ACHBFW_002420"
     CDS             complement(5352..5540)
                     /locus_tag="ACHBFW_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM9318688.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(5553..6119)
                     /gene="amaP"
                     /locus_tag="ACHBFW_002421"
     CDS             complement(5553..6119)
                     /gene="amaP"
                     /locus_tag="ACHBFW_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM9318689.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         1935 a         1335 c         1084 g         2034 t
ORIGIN      
        1 ataaattcaa gatcagccag ttctgtactc gtgaatatac cattaaaata ataacttaaa
       61 aactttaaaa aataagattg accgtacaga aactgtgtga gctcagaaag agcaatacca
      121 tgtttgaata cgagtttata cttatgcttc tctcttactt gttcttttat acaatattct
      181 agacactcgg ctagactttc tagatcgtga aggatcgttg gtgtagtcac ccttagagaa
      241 atagctgttt gttcgatgtc tatttcttgg aattcaaata attgttcaca cagatacact
      301 tttctaaata tatttcgctc gatgtaattt tccagcattc ctgatacacg cctccagttt
      361 ttcttatgat tttatttaat catatctgta aatcgtattt ttgtttcctt tttatttaga
      421 aaagcccttt caaaaaccaa taaaaaaaga aactgggaca aaagtcaaac aagctcacaa
      481 tttcagcctc attgtaggta gctttgagcc gtttggactt ttaccccagc ctctttcaag
      541 aatggttatt caaagatgtc agcgtctagt tctttgacta catctttcaa caattggata
      601 ccagctgcgc tgttaccaaa tggatctagt gccgggctga agacacctat gccgtaacga
      661 tttggcacag ctgccatcaa cccgccgcct acaccgcttt ttgcaggtac accaacatgg
      721 acggaaaact ctcctgattc atcatacaaa ccagcagttg tcataattga tttgacgatt
      781 gtagcacttt cttcagtaat gatttgtttg ccattccaag gagcaattcc tttatttgcc
      841 agcacagcag ctaattttgc tatgcctgta gcggtgacat tgagggagca ttgtctgaaa
      901 taagcatcga gtatctccgg tacgtctcct tcgatcattt gattaccttt catatagtaa
      961 gcaagagagc gattgatatc acctgttctt gattcagaat gatagatttc ttcattcaat
     1021 gtgatattag gatcatcaca gatctctttc ataaatgaca agattcgctc aaatttttca
     1081 tctgcatctt taccggcaat caacgaggtt gttgcaatcg ctcctgcatt aacaaaagga
     1141 ttcattggat gtttgcgatg attgatttcc atattcatga tagaattgaa ggcaaaaccg
     1201 gttggttccg tgttgattgt ttgaaagact ttttctattc cgttgtcttt gattgctaat
     1261 aacaatacgg gaacttttga catgctttcg atggcaaagc gcacatcggc gtctcctccc
     1321 tcaatcagtt gttttgtatc caaatcataa atcgccatac ctaattgttt gggatccact
     1381 tcagctaatg ctggaatgta atccgccact tgtcctgttt gataatagtg gaaatttttt
     1441 ccaatcaatt gttctaattc ttttacttcc atttgaataa ccagctttca ttttttattt
     1501 ttctgcatgc tggaatttat gacggcatgc gtagatcgta aatggaataa ctaagacgat
     1561 aagataagaa acaatcagaa tcgtttcata aatcccatgc tgtccagctg cgatactagc
     1621 aggtggaaca aatgagatac aaaaagcaaa aagtgaagtc aaaagaccta aacaagcaac
     1681 gattactttt acaacttttc cgcctggaac ctgataggct ctttcaagac tgtcatcttt
     1741 aaaaaccagc ttcaaatagc ccgcgaaaaa cataaagtac aacatcaggt agattacaac
     1801 agtcaatgac atcgctgcaa gaaatgaaag attattcccc ccgccgctta atgtaaagac
     1861 ggctgcccag attgttgcaa cgatcccatt tgcgatcatc aaatgagcag gaacaccgtg
     1921 tgtgttttct tttgcaaaat attcaggaag taatccttct tctgctgcta cgtgaagtgc
     1981 ttttgaagga gaggttaccc aagaagcaat ttctccgatg acacctaaac aaacaagaat
     2041 cgcaagaatt ttcgcaatcc atgcaagatt tggatcaatg tgtcttaata ggaattctaa
     2101 cgattgaatc acccctgtat taagagacaa accatgttga ggtactgtag tagcgacggt
     2161 caatccgcca aaagtatcta aaatgattgc cagaataacc aatacaaaca ttgctaatgg
     2221 ataatccttt ttagggtttt ccatttcatt tgcatgactg gcagatgttt ctacgcccat
     2281 atatgctaaa ataaaggaaa caaaaacaac cagcgtaggc aatttcgaaa aatcaggaat
     2341 caatgttttc atagacagta tcgtttgtag tggagcgcct gaagcgacat aatggatccc
     2401 taagccaaac aagactaatg ctggcaacaa gataccagct acaaaaccga tccgagcaat
     2461 caatgaagtg tatttggttc cttttaactg tgagagtaaa atagcccaga aaataatcaa
     2521 taggattgcc aatttcagat atttattgtt ttgtatagca ccaatacccg ttgcatagga
     2581 taatgcacca gttaagaaat acagcatggt caagtagcca actgttatct caaaccattg
     2641 gaaaaagatt gcggcaaaac cgaatcgttt tcccaatgtg cgactgaccc aagtatagac
     2701 accgcctttt tcccatcctt tcaccgttgc catctctgct gcgcacaatg ccacagggat
     2761 aaaccatagc aaaccgccta ataacaagaa aaatactaag ctgaagcctg aggtcgcgaa
     2821 tgtgggatat tcatataatg aaacgaccat tgagatggtc atagccaaaa agccaaagaa
     2881 cgttaatttt tttgcaggaa ttttcgtttt gctttgttgc atgaatcttc ctcttttcta
     2941 tttgaatttg tttcaattga gtttgttgcc aaagaagaaa aagtccaatc cttcttcttg
     3001 ttcttctttt gtagtagtga tcgttgtaaa gtgcatcgcc gattttaaaa tcactgttgt
     3061 cagcacatag gcatacacgg cagtaatgat gacaccaatc acttgaatcc ctaagaagta
     3121 ccagctgctt ggattcgtta gtaaatggct gttagatgca aagatcccta tcagaatcgt
     3181 acctgtgaat ccgcccatgc catgaacacc ccacacatca agtgcatcat cccattgttt
     3241 ctttttacga aaatcaacag cagcatgaca gacgataccc gcaatgatcc caatcagcat
     3301 ggcacttttt gcttctacat aaccggcaca aggcgtaatg gctgccaatc cagcaacgga
     3361 tcctgttagt acgtcgacaa aatcaaagcc atttcctttt acttttgcaa tgatcagcca
     3421 agtaatcatc gcaaatgcaa gagcgatgaa tgttgaacca aatgcattga ctgcttgttc
     3481 atctgctcga agtgcaccac cggaattaaa accgaaccag ccaaaccaaa gcagtcctgt
     3541 accgatagca gctgccataa gactgctatg ctgacttctt tctcctaaag gaatctttct
     3601 ttgaccaagt gtgaggatac atgctaaaga gccgaatcca gctgtcgtgt ggatgactgc
     3661 tccaccagcg aaatctctaa atcccatctg atccaaaaat ccgccacccc atatccagtg
     3721 gcaaacaggg aaataaatca gcagattcca aaaaataaca agtataatat agccttttaa
     3781 attcaatctc cctgcaaaag ctcctgtcat caaaggaacg gtaatcacga taaacatcag
     3841 ttggtaaaga aagaacatta aaaatggaat cgttgcacca tgaaacatgt tggggaagct
     3901 tgtaacatgt ctcatcaaaa agaaatctgc cgggtttcca ataacaccgc cgatatcccg
     3961 tccaaaggcc aatccgaatc caccaaatac ccacataatc gttgttatcc caacggagag
     4021 aaaagattga gacatgatcg ttagtatatt attcttccgt gctaatccgc cgtaaaaaaa
     4081 agctagacca ggagtcatca ggcaaaccat tgctgtacat aaaatcatga atgctgtatc
     4141 tcctgtattg ataggtaatg tattcatttt tttcgcctcc tttctacgat tacgagtata
     4201 aaagaaggtg tagttgtttt tttcttctgt ttttttgaat taatacttgc atttataaaa
     4261 gacagacaat taataaaagc ttgatgtgtt gtgctctttt tcgtttttca cacaaataaa
     4321 gacaaaaaat aaacggtctt ctttttatag aaaaccgttt attttcgtct ttttttattt
     4381 ttaaagatat tttttcataa ggaataaaga atacttcata taaaatatat aaaaaagaac
     4441 cgcagcgagt gtcgctacgg ttctcaaatt tgttcttcat ttcaacataa gtttttcttc
     4501 aatttgctta taatttacgt gcaaagaatg aaacgactaa tactaaaatg attgcaccga
     4561 tcaatgatgg aatgatagcc attcctgcaa ttgttggtcc ccaagttcct agtaaagctt
     4621 gtccaattgc tgaacctact aaaccagcaa tgatgtttgc gatgatcccc attgatgatc
     4681 ctttattcgt gattgctcct gcaattgctc caatgatacc gccgacgatt aatgcccaaa
     4741 taaatcccat tttaaattcc tcctaactga cttcttactt cttattgtac acgtggttcg
     4801 ttattttctt tgactttttg tgcacctttg cctacagcag attttgcttt atctgtttgg
     4861 cgtgaagcaa attgtcctgt ggattttgct gcttctgtta ctttgtcttg tactgtttct
     4921 tgatcttttt ggtattcttc ttgtgttttg atgtctgcaa cattcacatt cacttcgata
     4981 acatctaaat gtgtcatatt gtttacttca gtactgatca attctttgat ttggttgtaa
     5041 atatcttcga tgtctttacc gtattctacg atcacatcta gatccacagc tacttgtttc
     5101 ttacctactt ctgtattgat acctgctgtt acgttatctg tgttgactaa tttttcagcc
     5161 atatttgaga aaaatccgcc atctaatgtc aatagaccat ctacatcttt caatgcataa
     5221 ccgatgattt tttggatgac tttgtcttca taagtcaatt caccattcat catgcggttt
     5281 tctccttttt catttgccaa aactgatcca tttgttgtat tcatgttttt gttttccatc
     5341 tttgtttcct cctatgattt aaaaaattga tcaactattc ctgactgttt caaataaaaa
     5401 ccgattcctg cgccaattgc tgtcatgatt atgatcaaca atgtcttgaa aaatccaatc
     5461 gttaaccata aaacagctag cagtagtcca agacccccaa agataatagg gagcttgtat
     5521 gctgaaaata gttctttcat tatttggccc tcctattcca ctcgagagtg cttgtagtct
     5581 cttgtatctt cttgttcttc ataaccgctg tatgtgactg ccactttgac cgtttctttt
     5641 gttccgagta ctttagttac ttcttcttcg atatcttgca tcaatacacc tgttttccct
     5701 actaatgaag aagttcgttt caagtctccg ttgacatgga tatggattcg atttttcgtt
     5761 gaacgtatcc gtactttagg tgaatgaatg aactcttctt cgtgtaaatg ggaacgtacc
     5821 aacccttcga tcgctttttt gtcgattgtt agtttgccat cttcccgttt cataacgaaa
     5881 gttcctcttg actttggata gaaaagaacg actagcaaga tgataatagc aagaacggca
     5941 aatactacag ctgcccagaa aatatattgt tgcatgtagt aattcgttaa agtaaagaag
     6001 cgaaatcgtt ccaattgttc aggcataata tacggcgcat tgatcaacgc aatcgctaga
     6061 aatactgaaa gtagcagtaa actcatgata acaccgatta tttttaaccc cttattcatt
     6121 ttccacctcc tataaactta tttttaccct gtaaataact ctaccgacaa tcacagtata
     6181 gatagcgaac agttagcata gcatcagtaa ataaagcgct accaaacatt gctggatcat
     6241 aacggcgttt tattgccagt ctgatactga aataacaatt gcttacttat tctctattta
     6301 cttttgattg ctggctatac ttaaaggata ttattcaaga ttaaaaattg caaagattat
     6361 gctcagttat tattacaaga ttctattt
//
LOCUS       DAYYME010000136         6294 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000136 DAYYME010000000
VERSION     DAYYME010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.137"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2485
                     /locus_tag="ACHBFW_002422"
     CDS             <1..2485
                     /locus_tag="ACHBFW_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFM9318690.1"
                     /translation="TYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKEGSATDS
                     EKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTIQNFLQL
                     LHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPDNIDRSN
                     CFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIKEGDHKG
                     VTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKYPLPKPV
                     NPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQKVITLVL
                     AYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENAPIFFEH
                     ESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKYVLGTNL
                     ELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGNPTTVLR
                     NMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLDSNCQLM
                     PQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKSLSSNTF
                     GWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYSGALLIQ
                     DILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLTNSDNKH
                     TPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNMCAEKGR
                     RTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            2667..2906
                     /locus_tag="ACHBFW_002423"
     CDS             2667..2906
                     /locus_tag="ACHBFW_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318691.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            2922..4295
                     /locus_tag="ACHBFW_002424"
     CDS             2922..4295
                     /locus_tag="ACHBFW_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9318692.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4299..5933
                     /locus_tag="ACHBFW_002425"
     CDS             4299..5933
                     /locus_tag="ACHBFW_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9318693.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6096..>6294
                     /locus_tag="ACHBFW_002426"
     CDS             6096..>6294
                     /locus_tag="ACHBFW_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318694.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2200 a         1030 c         1278 g         1786 t
ORIGIN      
        1 aacttataat cctattaaac aagaggtgat tacacaaaaa aagcattctg tgagagacaa
       61 tgatattgaa aaaattcggt ttgtatttat tgatattgac cctaaaagga aagagggaag
      121 tgcaacagat agcgaaaaga aaaaggcaga aaatgttatg gaacaagtag aacgttattt
      181 gaaagaaaag agaatagaat catttgtaaa agttgatagt gggaatggct accatatttt
      241 tcttcccata aatgaacaac caaataatca cgagacgatt cttactattc agaattttct
      301 tcaattactt caccgaagat ttggagtaga agatgaagtt gatattgata cttctgttta
      361 taatccttca agattatgca aattagttgg tacaccagcg gtcaaaggaa agtccacaaa
      421 agagcgtccg catcgtttat ctagtttcct ttctattccc gataacatag atagaagtaa
      481 ttgttttaaa attcttgaac agatagttga agaaaattcc atgatgtcaa cctctagagt
      541 aaaaagccat ccatctttta gagaagtgtt accttttatt caagcagact ataaacaatg
      601 gttagctcac tatcctgaat taacttatac cataaaagag ggcgatcata aaggagttac
      661 cttacttata tttgacgaat gtccattaag aaaacacacg aataattcta atggttcttg
      721 tttgtctgtt acggctgata ataagattcg atttcattgt ctgcatgctt cggataaaaa
      781 taaagatatt caagactttg taaaaaaata tccgttgcca aaacctgtaa atccttctgt
      841 agaatcatta gaatccaatc aagaatatta ttttgaccag tttaaactga atcaagaagg
      901 agtatttttg gtgaaagaag gtcaagaaga aaaagttgct tgtccgatgt ttattaagca
      961 aatacgcaaa aatatcgaac aaaaagtcat tacacttgtt cttgcctatt atgtcgatca
     1021 ttgttgggat gaaatagaaa tagaaggtgg atgtttacaa actaataatt ttaaacaatt
     1081 agcaaaacat ggcttaatca ttaaagctag aagagaagct gaagtcgttg actttttaca
     1141 gattcaacgg gagaatgctc caatcttttt tgaacatgaa tctcttggct ggaaaaaaga
     1201 aaatgaagca atgatttttc agttacaaga ttcttttgga cgtgaaattc cttctgtatt
     1261 aactaaaaac tcatgctacc aattaacaac taatggtaca gaagcggaat ttgacgcatt
     1321 ggttcataag tatgttttag gaacgaactt agaacttgct tggtcgattg gtattgcttc
     1381 tattgtgtta ggctatttaa acgttacgaa ggctaatact tttttgagtt tactaattaa
     1441 cctacagggc aagagcacaa cgggtaaaac cactacgtta cactttattg cctctttatt
     1501 tggaaatcca actacggttc ttagaaatat gaatgctacc aataaagcca ttataaagct
     1561 agcatcaaac aatcagggag gtgttccact aatactagat gagttaggag cagcaactca
     1621 aaataacttt agctcgttga tctatcaatt agcttcagga gaagaaaggc taagattaga
     1681 ttctaattgt cagcttatgc cacaagagac tttttctgtt ttgaccctta cctcttctga
     1741 agagcgtttg gaaaattatt tcaatgagaa attaactggt ttgaaagttc gatatatcga
     1801 attttctgat atcaagttta caaaggatgc gaatcacgca gagaaagtta aaagtctttc
     1861 ttctaatact tttggttggg gtataaagtt atttcttttg aaactctttg aacaagggat
     1921 tacaaaaatt gaagcaactt ttgtcgaagc tcaaaaaaaa ctatgggctg ttttacctga
     1981 acattctttg aagcaacgca ttggtaataa tctagctttg atttatagtg gagccctcct
     2041 aattcaagat atactgggct atcctttacg attagagcat atacagacgg tgcttattaa
     2101 ttcgtacgaa cagttaattg tagatactag tttaggaagt gagcaaacgg tatttgaccg
     2161 agtgaaaaca ttgttggtga tgaatagcca taaattttta acaaacagtg acaacaaaca
     2221 tacgcccagt acgatttggg gaaaagtatc aattcaaaaa aatggggata ttaaggtaaa
     2281 tatttttcct atcccgtttg aggacttgtt aaggcgagaa tttcaagtaa aggatctaac
     2341 ctatcttttt aggcaattgc ttgattcggg taatatgtgt gcagaaaaag ggagaagaac
     2401 gaaacgtatt cacatgaatc gtcaagttct tccaacatac gagattctat taccttcttc
     2461 actcaaatcg tattttcgat tgtaatttag aataaaccaa agtatgaaag tgaggcacca
     2521 tgttatggaa atatttctta ttgatttagc aaaacaactg attacttatc tgtatgatag
     2581 cgagcaaacc caaacggaag taaatgaagc ttgatctatg cttcatttac ttcattcaat
     2641 caaacatgaa agaaaggaaa attctaatga ctaaaaataa aatgacacta aaagcagagg
     2701 ttcttctgta tattcaagaa catttttcaa atcaagcctt ttttacaaaa ccaatttact
     2761 tggatttcga aataagaggg gtatctgctg gatctatcgg gggtactctt caagcgttga
     2821 agaacgaagg atatttagaa aatcactttg tgcaacgttc gttcaataga agagtcgtga
     2881 aaaaatggta tttagttcat tcatagagag gggaatcaag catgcgtgtg gcttgttatg
     2941 atcgaacgtc tgtttatgaa gaagcgaaaa ttagtaatga cgctcaatat gatgcagtga
     3001 aagagtatgt tttagagaaa tatgaacaaa ttccccaacg attttcagac aaaggagaat
     3061 caggggaaaa agcggagcgt ccagcttttc gacagcttag agcttgggtt tgtgcgtcag
     3121 aagaagaggg ggtgatctat gtttggcgtt atgatcgatt ggcacgtcaa gccaaaatag
     3181 ccttagaatt tattgagttg tgccaacaac atcaggttca agtgtattcg atttctgaac
     3241 ctcttcctgc ttctttctca cacgcgtcaa cgaataaact atttgtgcaa ttattgttca
     3301 cttttgcaga acaacaacga aacgtcatta ttgagaatgt tcgtgccgga ctttcctata
     3361 aaaagcaaca acggcaatat ttgtcttctg cggtaccata tgggtatcgt ttagtgaatg
     3421 ggaaaatcca acaagaagta cacgaagcaa ggattgttcg ctatattttt caactttatt
     3481 tgaccaaaaa atatggctac aaaaagcttt gtcaacgatt gacgcaacaa aagcttttct
     3541 tccgcgaacg tccttttcag ccctatcata tttatagtat cctaaaaaat ccattgtact
     3601 atggcgaaat caaaggagga tctttgggga agtatctagg aacctttgaa ccgattctct
     3661 cgaaagcaac ctttttacaa gcccaagaga ttcgccaatc aaggtgtaca gctaaaaaag
     3721 atacgtatct gtacttatta cgtcaaaaaa ttcggtgtcc cttctgtggt cgtcatctga
     3781 gttcgaagta tcaatggaat acaaagaaga cgaagacgct tcattattat cattgtaccg
     3841 accgaagctg ccaaggaatt tttcttcggg cagaaagggt tgagtcagaa gtattggaaa
     3901 agatacaggc tttttttcaa tacgaaggcg tctatcaagc cttgattgcc caattaacgc
     3961 aacaattagc cgaaaagcga aaagatgaaa aacgcaaagt aatccaacaa aagaaacaag
     4021 aagcacaatg gattaaggca tttgaagagg gcaattgttc actggaagaa ctcaagcaac
     4081 aactgcaagc tcttcaaaag ccgatgtcta cttcttcgtt tctttcactc caacagtgtg
     4141 aagcaaaagt gaaagaatgg cttcaactaa aagacctctc tagtcgacaa ttattatgtg
     4201 actatgtttg tcggattgag gtaacaaagg ataaaacagt ttgtgggatt tacttccgac
     4261 aactgacaca aaatatttta gaaaggaacg aatgatctat ggaaatgata caaaccaaac
     4321 gagtcgcatt gtatattcgc gtgagtacac aagaacaagc tttagaagga catagtattc
     4381 aagcacaaga acaaaatgga cgacagtttg ccgaacggat gggctatgaa gtggtagaag
     4441 tttatgccga tgaaggaatc agtgggaaat ccacaaaaaa tcggttcgcc tatcaacgaa
     4501 tgatgaacga tgctcggaac catcgttttg atctactagt gatttggaaa ttgacccgtc
     4561 tcggacgcaa tatgttagat gtcttacaaa cagtcgagga attattgcga tatgacgtag
     4621 gattacattc gatctcggaa caatttgacg tgaccacttc gacaggtaaa ttgatgttac
     4681 aactccttgg gagtttcggt gaatttgaac gcaaccaaat tgcggagaat gttcaaatgt
     4741 caatgcaaag cttagtgaaa gataaaaagc gttatgcagg aggccgacgt ttaggctatg
     4801 tcagtggtat tgacacgaat ggtcaaaaac aattggtcat tgaaccagaa gaggcgaaaa
     4861 ttgttcgtct gatctatgca aaatatttag gcggagcagg gtatcgagcg attgccaatt
     4921 ttttgaatcg tcaaggatat caaacagtca aaaagaatgc tttttctacc acggcagtca
     4981 aagatatctt acgcaataaa atttatgcag ggtatttaga atatgcgcgc tacatcaatt
     5041 gggaaaccaa acgacggaag gggaagaatc cgagaccgat tttagtgaaa ggaacccatg
     5101 aaccgattat ttcggaagaa gattatcaaa aagtacaaga acgccttgct ttagaaggaa
     5161 aacaacccaa atggaaccat acaggtgaaa atgtattgac aggcttgtta cgttgtccag
     5221 aatgtggcgc accgatggca gcgagtaatg tcacaaatac cctaaaggat ggcacaaaga
     5281 aacggattcg atattattct tgttcagtct ttcggaataa aggagcaagt gtgtgtcatg
     5341 ccaattcgat tcgtgccgaa caagcggaaa catttgtcgc cgaccgatta aaggagatcg
     5401 ttcagctacc agaagttctt ccccgattgg tggcagcgtt aaatgaagaa atagtgcgac
     5461 aaagccaacc attggaacaa gaactcgtgg tgttactgga acgaaaggaa gaattgaaga
     5521 ctaaaattga aaaatgggaa gctgctttgg aagatagtcc tgaattgttt ccgatgttaa
     5581 aagaccgctt agacgaattg actgaaaaac gacgacagtt gcacattcga gaaaatgaaa
     5641 tcttagggat ctttcaacaa ggagagccga ttcaagtcaa agatgtccaa cggatattga
     5701 ctagtttgga tcgttttctt gcccagagtg agaagaagca aatcaaagcc ttgtatcgta
     5761 cctttataga gaaaataacc tttgatccta accataagga gatgttggaa attacgatga
     5821 aattcacgca tgccgtggta caacaattga atgagcaata tcaaatagcc gtatccaaaa
     5881 caaaggatac ggctattttt gtgttaaaaa caccctttac gttaacgatc tagtctaggt
     5941 tatatcaaac aacgttagag gggcgctaac gcttctctag cgatttgaaa tcgttagaga
     6001 agtagttagg gaggtcctta tcataaatag ggatatttat taatgaatat ttgaaatata
     6061 attgtatata agaattaaaa agagtgggga tagatatgaa aaagatgggt aagtgtattt
     6121 tatgtgaaaa atacactgaa cttactaaag aacatgtacc acctaaaaaa tcagggaata
     6181 ctggaggtaa aaagaccaga actggtaatt tagatgattt ccttaagagc gattttacaa
     6241 aaggtgattt tccgaagggg attaaaagga aacctcaagg aaatgtttat tata
//
LOCUS       DAYYME010000137         6280 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000137 DAYYME010000000
VERSION     DAYYME010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.138"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..330)
                     /locus_tag="ACHBFW_002427"
     CDS             complement(1..330)
                     /locus_tag="ACHBFW_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFM9318695.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            507..1235
                     /gene="sfsA"
                     /locus_tag="ACHBFW_002428"
     CDS             507..1235
                     /gene="sfsA"
                     /locus_tag="ACHBFW_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFM9318696.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            1251..2264
                     /locus_tag="ACHBFW_002429"
     CDS             1251..2264
                     /locus_tag="ACHBFW_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9318697.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            2264..3541
                     /locus_tag="ACHBFW_002430"
     CDS             2264..3541
                     /locus_tag="ACHBFW_002430"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM9318698.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3604..>6280
                     /locus_tag="ACHBFW_002431"
     CDS             3604..>6280
                     /locus_tag="ACHBFW_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9318699.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQM"
BASE COUNT         2096 a         1072 c         1332 g         1780 t
ORIGIN      
        1 ctatttttca gataacaact tatatgaagt gtctgcagat tcgtcataaa gagtttgttc
       61 gggataagaa aagataagat aaaaaccata ctgatcacgt acgactaaac gatattcatc
      121 tatttctacc acagatgtgc caagagactg accgttgatc aaaaacgtgt aatcagatgt
      181 aatctgtaat tgttgttttt gctggctatc actaaagaac catgtacctt gatactttga
      241 gagtccttct gacttttccg cttgtttttt ctttttcaag taaataaaac ttgagagaac
      301 ggtaatgagt gaaccaagaa aatagatcat tttttttatc accaaatcac tcctttatga
      361 gaaaataatc gcatagacat attctttctt ctttatttca ttgtaccgaa taaaagctaa
      421 aatttctcta agactttcga ctggtttttt tactgttttt ctacaaatta tgataggata
      481 catagtagga agaaaggtga cataatttga agtatcagca agtggaaata gcttttttta
      541 ttgaccgccc gaatcggttt atagcacatt gtatgaaaaa agacggagaa ataattattg
      601 cgcatgtcaa aaatacaggt agaggaaaag aggtcctgat accaggcgct gaagtggcta
      661 ttgtatttgc tcctggacct aaacggaaaa ccgcctatga tctgattgct gtaaaaaaga
      721 agggtgactg gtttaatatc gatagtcaat tgcctaatcg gctggcaatt gatggtattt
      781 tagacggcac gatcctttta ccaggattaa ctggtccatt tacggtatac aagcgtgaag
      841 taacttatct tcactccaaa tttgatattt atttagagac ggtggtcgga caaaaagcat
      901 ttgttgaagt caaaggtatg acacttgaaa ataaagcgat tggtgctttt cctgatgcgc
      961 ccacactcag aggattgaaa catataggag aattaacgta tgccgcacaa gatggttatg
     1021 ccgcttatgt cttgtttatt gctcagttcg aacatcttca tctggcaacg atccatgaag
     1081 aaatgcaacc agcactagct gatatggttc gacatgcgca gcaaagcgga gttcaaatcc
     1141 tcgcctataa ttgtcaagta tcggcagatg aggtaactgt caagcaggcc attccatttg
     1201 atgtagatat cccttttgaa gatcctatac aatagaaaga aggtaaaata atgatcaact
     1261 taggtattat cggaacaaat tggattactc atcaatttgt ccaagcagct ttaagcacaa
     1321 aaaaatatga attgacagcg gtatattcta gacatttgga aaaagcacag gaatttggtt
     1381 ctcattatga aggggatatc gaattcgcaa cagatttgaa gacttttttc aatattactc
     1441 atatggacac catttatatt gcttctccga atagtcttca ctttagtcaa gctaagcaag
     1501 caattttggc aggaaaaaat gtgatagttg aaaagccagc tttttcgacg ccaaaagaaa
     1561 tggatgaaat catccatttg gcaaatgaac acaatgtgta tttttttgaa gcagcaagaa
     1621 atatccatga atcaggtttt aaaaagattg ccgattactt accattaaaa gacgagataa
     1681 tcggtgcaaa tttcagctat atgaaatatt cttcgcgcta tgaccaagta ttagatggag
     1741 aagaaccgaa tatcttctcg actcatttct caggtggtgc attgatggat ttaggtgttt
     1801 accttgtata tgcagctgtt gcttggtttg gaatgccaaa agaagtccat tatttcccaa
     1861 gaaagatttc tactggtgta gatggtcttg gatgggggat cttacgttat gagaattttg
     1921 atgtatcgat ccagccaggt aaaatcggtg attcttattt accgtcagag atttattttg
     1981 actcaggaac gctagtgatg aatggagtca atgcaattga acaagcacaa tttcatgatc
     2041 gcgcgcataa agaaatcatt gatttggcgg taacagctgc agaaaatcca atggcagatg
     2101 aagcagagga ttttgccaat gtcatggctc atccgaaaga tccagcttgg ggattgttgt
     2161 atgaagagtg gatcgagcta gctagaaatg tgaaccaagt gatttatgat ttaagaaaaa
     2221 atggcggcat caaattcgat gcagataatg aggaagattt ttaatgaacg aatttgtaca
     2281 agtatttccc gaaaattggc agaaaaaata tgaggataaa gggtttatct caccttctct
     2341 tattcaacga gcagtttttc agccactaag taataaacaa agtatagtag cggtttctcc
     2401 tacaggttca ggtaaaacac tggcttatct ttggcctcta ctactaaacg tggaaccagg
     2461 tgaagcaagt gcgttggtca tttttgcttc ttctcaagaa ctagctattc aagtagcaga
     2521 tgttgcacgt gaatggggaa aagacaaaga gttgaaagtc caatctttgg tgggcggagc
     2581 caacgtcaaa cgtcagattg agggtttgaa ggaaaaacca gaaatcttga ttggtacacc
     2641 aggaagaata ttggaattga tgaaagcaaa aaaaataaaa gcacatcaag tgaaaacaat
     2701 ggtatttgat gaagcagacc agctatttga cgctgggaac agccaaatca ttgatcaaat
     2761 tcttcatcaa gctccaacag agtatcagct agcttttttt tcagcgacag cagatcgttc
     2821 ccttgaaagc attgaaaaaa tcactgggaa aacgtttgag acaattgacg tgacagcaga
     2881 ggacgattct cgaaaaggac tgagacatta ctttttacgt gtaccgatac gaaagaaaga
     2941 tgactacttg agacgtctca cgcatataga agattttcat ggtctgattt tctttaatca
     3001 actgactgaa ttaggcgtaa tggaagaaaa attgctgtat agaggtgtac cggtaggaag
     3061 tctggcctct gatcagaaca agctactccg aaaagcagcg ctagaacaat tcaaaaagaa
     3121 aatggtcaga gcacttttga ccacagatgt ggcggctaga ggtttagata ttacaggtct
     3181 tccttatgta gtgaatgcag aagttccgtt aagtgaagaa gcttatttgc atcgttcggg
     3241 aagaactggt cgaatgggta atgaaggaat agtaattaca ctcgtacaag ataataatct
     3301 aagagattta aaacggcttt tacgtccatc aaatataaca cttgaagaga tttttttata
     3361 cggaggtaag ctgcaaactg agcagccaac caaagaagat gcagctcctg cagtaaataa
     3421 aaagtatgcg aaaacttttt ctggaaagga gcagaaccca gaatataaca aagaaaaaat
     3481 aagaaaaaaa gttaaaaata aatcaaaaaa agagagaaat aaaggagcaa ggcgcaaata
     3541 agcgcctttt tatgctatga ttatgaaaac gggaacaaaa ttggttgcca gaggaggggt
     3601 tatgtgaaag aacgtcaaga agagatcttg cagctattga cagaaaacca acaaggattg
     3661 acagcaagtg atgtagctga gcgcttaact atcgatcgaa gcaatgcaag ccgatattta
     3721 agtgaactat acaaggccca tcatattgta aaaaccgctg gccgaccagt cgtttactct
     3781 ttgccaacgg aaaagtcaaa atctgatgaa gtgcatgtcg actcttctac gcaagtgact
     3841 tttgaaacac tcgttggaga aaatgattct ttaaaagtca gcatacagca ggcaaaagct
     3901 gcgattcttt atccaccgcg ggggttgcat acgattattt ttggagaaac tggtacaggg
     3961 aagtcgatgt ttgctgaatg tatgtatcat tttgcaatcg attcagagat gctttctgca
     4021 gatgcgccat ttgtttcttt caattgtgcg gattatgctc aaaatccgca actgctcttt
     4081 ggacacatct ttgggatcaa aaaaggggct tacacaggag cagctcaaga tagtcccggt
     4141 ctaatagcca aagcagacgg cggtatattg tttttagatg agatccaccg tttaccgccg
     4201 gaagggcagg aaatgctgtt tactttcatt gataaaggta tttaccgtcc gcttggagaa
     4261 agtgcacaag tacatgaagc atctgttcag atcattgggg cgacgactga atcttcagaa
     4321 agtttcttat ctacgttcaa tcgacggatt cctatggcaa tcaccttgcc gaatcttgct
     4381 tcacgttcac tggatgagcg ttatgaaatc atctctcttt ttatcaaaca agaagcgaat
     4441 cgtttgaatc aacgtatcga tatcgaaaaa gaagcgattc ttgccttcat gctttatgat
     4501 gcggaaggaa atatcggtca agtgaaacga gacttgaaac tagtctgtgc aaaatctttt
     4561 ttacattacc gtactcatca tgagaaaaag ctaatcattc gaaaagaaga actttccctt
     4621 caagtacaaa aaggattact gaaaatcaag gaaataccgg aacgtctcga ccgattcatg
     4681 gacagcaaaa gtcagtattt gacatttgaa ccaggatttg ctgatgttgt ctggtcacaa
     4741 gatccagaac gaaacatgca ggtctataac gatattgagg aaaaagtcac ttctctttcc
     4801 gaaaccggtg tggagaacat tgatctagag accctcattt caaaagatgt ggatgcttat
     4861 ttccagacat atgtcaaaga attgactaaa agttcgattc ccaaagatct tttgccagat
     4921 gaaatctggc ggttaaccaa tcaattgtat gatgtggctg aaaaacggtt agaccgtatt
     4981 tataatgaaa aagctcgttt tgcttttgct ttgcatctgc aaagtacatt ggatcgtgtg
     5041 agagaaggtc atatgatcgt ccacccagat ttgaataacg tacggaaaaa ttttaagagg
     5101 gaattccagg tagcaatcga tctttcaacg atcattgaag aagaagaaca agttgagatt
     5161 ccttttgatg aaatcggctt tattagtatg tttctttcta tcaatctagg agaaagtgag
     5221 cctgttaaag aaaacaaagt agatgttctc atattgatgc acggcagaca gactgcttca
     5281 agcatgttgg agatggcaca agaactatta ggaacaaaag tagggaaagc ttttaatatg
     5341 ccactggtta tggaagttca aacaatgtat gaagaagtgt tacaatatgt caaaaactat
     5401 caagagcatt tgacgaatgg cctactcttg ttaacagata tgggatcatt aaacaccttc
     5461 agtgatttga tttatgaaga gactggcatt cggacgaaag ctatcacgat gacaagtacc
     5521 atgattgtga tagaagcgat acggatggca agtgttggaa gaagcttaga agatatttat
     5581 caaaatatcc agctttcctt tgaatcgatc gtacgtgatc agttcaaagc ggaaaagtct
     5641 gaaactttac agcgtaaaaa agccgtaatc gtgacatgct ttactggtga aggagtagct
     5701 gccaaattat atcagcgtat tatgccagtg attaatcaaa ataaggtaga actgatccag
     5761 atgcagtttt tagaacgtga atcattcaaa aaacacatcg atgccttatt ggaagaatat
     5821 gagatcaaag caattgcggg aacagttgat gttgattatc aaaatatccc atttttttca
     5881 gcctatgacg tttttgatga tgaaaaacta aatattttaa agcgtatagc tgatgatgag
     5941 atcccgacag ctaaaattgt cgattcactt gctggatcat taagcgatgt cttttctatt
     6001 gatgaactga tccgttcatt gcaaaagaca gttcagcaga tccagaccga tttgtcgatt
     6061 atcgtggaac ctggagtaga tactggaatc gttatccatt tagccttcat gttagacgga
     6121 ttgatcaaac aagaaagcca tcggcaattt gagaaactgt ctattttctc aaaaatttat
     6181 cgtttggaaa tggatatcgt tcgggcaaac ttgatggcac tagaacgaaa atatcaggta
     6241 aaaattccag aagatgatat cgcatttctt acacaaatgt
//
LOCUS       DAYYME010000138         6189 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000138 DAYYME010000000
VERSION     DAYYME010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.139"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(40..546)
                     /locus_tag="ACHBFW_002432"
     CDS             complement(40..546)
                     /locus_tag="ACHBFW_002432"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM9318700.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            706..1146
                     /locus_tag="ACHBFW_002433"
     CDS             706..1146
                     /locus_tag="ACHBFW_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM9318701.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(1172..2200)
                     /locus_tag="ACHBFW_002434"
     CDS             complement(1172..2200)
                     /locus_tag="ACHBFW_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318702.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(2255..2329)
                     /locus_tag="ACHBFW_002435"
                     /pseudo
     CDS             complement(2255..2329)
                     /locus_tag="ACHBFW_002435"
                     /inference="COORDINATES: protein motif:HMM:NF016901.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2332..2688)
                     /locus_tag="ACHBFW_002436"
     CDS             complement(2332..2688)
                     /locus_tag="ACHBFW_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318703.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(2986..3960)
                     /gene="sgrA"
                     /locus_tag="ACHBFW_002437"
     CDS             complement(2986..3960)
                     /gene="sgrA"
                     /locus_tag="ACHBFW_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFM9318704.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(4156..4995)
                     /locus_tag="ACHBFW_002438"
     CDS             complement(4156..4995)
                     /locus_tag="ACHBFW_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM9318705.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            5180..6067
                     /locus_tag="ACHBFW_002439"
     CDS             5180..6067
                     /locus_tag="ACHBFW_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFM9318706.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
BASE COUNT         1987 a         1223 c          915 g         2064 t
ORIGIN      
        1 accagtatcc attctgcttt ttttaaacga agaaacaact tattgctcca aaaatgtcag
       61 aaatcgttta tcccaaattt tctcgtaaaa agcgattgtt tcctttgctt tcataaaagg
      121 attatcataa gttgtcgttg ctcctctaca catcataagc tggtatccta atccatgagc
      181 aaacttcaca gtcgtatcta tacaatactc tgcttgtgcc ccacataatt cgatcgaatg
      241 acagtccaat tcagttaata aaacactcaa attagtctta taaaatgaat tcgcatgggt
      301 ttttctcaca aaataactgt gctcaggaat agataattcc gacaatagct cccatggttt
      361 tgtttgtggc actaattgag catcttcgtg ttgaacgaaa ataactggtt tcccaatcgt
      421 ttcgtatgtt ttgattcttt tattcactaa tgctgtcact gtttctaatt cgtgtatcgg
      481 cgaagaatca aaacataccc ctttttgtaa atcgatcact actattgcgt cggctgtgtt
      541 tgacatactt gtgccttctt tcttttttct tgatttaata gtatttctta tctagctagt
      601 tccattatac gtgacaacat tttcttattt tacacctcta aatggcactt ttcgactatt
      661 tcttcatgtt attatttcat aaatacttag ctattggagg aatgtatgat gattaaattg
      721 tatacaggtt ctggctgctc gtcttcaaga cgcgcaaaac gttggcttat cgtccatgat
      781 ataccgttta tcgaacagaa cttaattcgt caaccactaa ccaaaaatga atttatccac
      841 ttactaaaat tgacaaacaa cggcgtgacc gaaatgctct ctactcattc tattagttat
      901 aaaaatcttg ttcacagaat agatgaactc agtttgagtg aactatatat tgagattcgt
      961 acatcccctt ctttattgaa aaaaccgatt atctacgacc aatctgcaaa acttttgact
     1021 ggatttagcg aagataaaat caggatgttc ctttctaaaa ctaaaagaaa gcaagagctc
     1081 aacgaactct tgctttctgg tcatcttgtc gattttaaag aaaataatct ttttgcaatg
     1141 tcttaatttt ggcataaaca atctgctttt cttaattaac gaaatccaat ttttccaata
     1201 tggatctatt tttctcattc ctgattttta tgatttcagc tttcagacgc gcccaatcat
     1261 tttttgtagg ttgtccattc cagtaaaata tctgtgtatt cgtttgatca tatgcctttt
     1321 tgtcgatagg ataatcgcta atgacaatat caggtaactg atcagcggat gcactatatt
     1381 ttattgggcg ttcagtcata gataatatcc ttttctttgc ccataattcc tgtgttttac
     1441 catactttga ttgaatatag aatatgacag gttgttcgtg ttttataaaa atatccctga
     1501 ctaacataca gtattgaaaa atcaaccgtc tgttcttttc ataataagta ttaagctctg
     1561 gaattctgcg agctacccca ctaaaaaact gttgcatgat tggatacatc actggaaata
     1621 tatgctgaaa atctctgtct tctgctgatt ttccaaaagc atccaccttc ttttttgtcc
     1681 caaaaaccct tgaatacaaa tggatcattg ccaaattgac atatagataa tttttttctg
     1741 aagttgtaaa acaaatcttg aagttttttt caaaattttc tacaatagac gattcaatcc
     1801 agccccaact ttcttgtact ttatctggag tatccaacaa atcttcttcc aaataggtgt
     1861 tcattacacc aaacataaaa taaaggtaag cttgctctgt tggagtgatg tgattcatcg
     1921 accagaagtt tgcttcaaaa aaaggctcta gtatgtaatg cgtaaactct tcttttgtta
     1981 tcaatggatg cacaaatttg ttcatctctt ctgggacata cgtaatcgag tggccttgtt
     2041 tcattcgttg caacgaaatt cttagtaggc tgatccattt ctttctatct atactacgcg
     2101 tatatggcgc atgtttcgca aatttttcta atagttgacg tttatttttt atacttgata
     2161 atatatcaga atgattttcg tcgaatgact gccaatacat caagtgaaaa aagtatcgga
     2221 tacggtattc ttctccttct atcggattac cttttttcaa gctgcaagat aaatgatagt
     2281 ttttcaaaat agtattaagt tctcttaatt ttctttgaat cgtacgcaaa cttatataat
     2341 tttttgacta agattttcta aggattgata agtacctttg taaagtgaat ccagtaaaat
     2401 ataaccaatc gttccctcta aaagtataga ataaacttta ctcagtaaaa actgagaaga
     2461 aaactcgact tttatcatct tgttttctga aagaaaagat aatttatatt ctggatacct
     2521 ttcgcaaaac gattctgctt gttgaatcac cgaattcatc ttagccaggg tgtagtttag
     2581 atctaaagcg atatccttca ccacatataa tttctcgtct ctttcataac aatagttcaa
     2641 aaattgatat acttgccttt cattgatatc aataaattct tcaaacaaag tacatactcc
     2701 tttttatcta ttatttagac ttattccgac agctacatga ataacgtaca aatagattcg
     2761 ttactgattt ttcagagata ctaccatgcg cagtgtgcat tgtcaattat tcttgctctt
     2821 attttccatt tgtgttcctg ttatttcctg tttctcaaat atgttagtaa acaaaaatag
     2881 atctggcaaa gctacaactt cgccaggcaa tacagcactc tcgaatgaaa tattcactca
     2941 aaaatctatc gttttattca attttagatc tgttgctttt attctttatt tcactcgatg
     3001 gaagagagaa cgaaaatagc ttgctcctac aatcaaaatg agtaacacac ccatccatgt
     3061 aattagcttg ttcaaggttc tactaccagt ttctggtaaa tcgctttttc tattatctgt
     3121 ttctttcgct ttttcttgat gatcaattcc acgagaaaca aaatcaggct gttttagcac
     3181 actttttagg ttgctccctg catttctctt ttgttcctta gttggtatga taggtaccaa
     3241 atcaagttca ttatttactt ttcctgtgct gctttcgctg ctcgtgcttc ctgtgctact
     3301 ctcactactc atgcttcctg tgctactttc actactcgta ctctccgtac tgctttcgct
     3361 gctcgtgctt cctgtgctac tttcactact cgtactctcc gtactgcttt cgctgctcgt
     3421 gttttctgtg ctaatttcac tgctcgtact ctctgtactg ctttcaccgc tcgtgttttc
     3481 tgtactgctt tcactacttg tgctatccgt actgcttggc ggtccaacaa ctgtccaact
     3541 gcttggtttc aaattctccg tgatcgtcat ctgtccatca taaaaaacaa tcaaatcaac
     3601 tttatcttta ctcttgatct catcaatcga tagttctgca gaaatcatta ttgtatactc
     3661 aataccagcg gtccattgta cgtcttttaa ataaacttcc ccaataactt tttctgaact
     3721 tccattacca tcccaaccat atgttagaac agcatgtaca tcactagcat tcacgctttt
     3781 gtttggtttg actggaatag taatgacttg ttgtgtcatg tcataaacaa tccgatcagt
     3841 tttcacagta aatgaaagat tctcatttgc ccgttcattt gcagaagtac tgattccaat
     3901 cattgctgca attatactca gcccaagaat aacaaataat acggtcgctg ttttcttcat
     3961 ttctatcact ctccatataa ttaccttcta tttgataaag gaattatata aaatgaaaat
     4021 gatgagaaca cgacagtcag ttgtgaaaat ctaacaaaag atgtcgtgta ttcgcttgaa
     4081 tttgtttact ttttcgttta atcaatgaca gacttaaaaa agcaagaccg atatcagcct
     4141 tgctttcttc atcttttatt gataaacagc agtgtatttc ttcatccgct ttgttttata
     4201 tgcatagagt aacaaaccaa caatcaagac taacaagaat aatgtagttg atatagatgc
     4261 ttcagcccca aagtttcccc cagtaattat tgtattttgc gattggatca gagtggagaa
     4321 aaaaccttgt tcttgcgttc cgctgactgg tatacccaaa atagctcctt gtgtccaatt
     4381 ccaagtaata tgagcaccaa tagccgtaaa taaacttcct gtactgtaga taagtgcgct
     4441 gaaaaagcaa cctgctaaaa agatattgaa aactgccaga aatgtcatgt taggattcaa
     4501 tccgtgcaaa agagcaaata atgcagcaga tccaatcatt gctattaacg gaccaaatct
     4561 aagcatctga tgctgaaaaa agccacggaa taaaagctct tctgatgccc ccactgcaat
     4621 aaataaaata aaactcgtta tcagcggcac aatgtttgcg tttcctgaat ctaaccgata
     4681 cgcccccgtc aaatataatg gactagtaat aagcagaaac actccaattc caacaacgag
     4741 acctcccgct aaataccccc agtcttttcc tcgaacagaa acaccataat cttctattct
     4801 ttttccttcg accttactag caagaaataa agctagtaaa aaaacagcta aatgtccgac
     4861 cgtaccggca attgctccaa tccattcact atttccaata aacatatccg caagtgcatt
     4921 attgacaaga attcctaaaa caaaaccaac aaagactatt aaaacagatc caatcaaccg
     4981 aataatcctg atcatctttc atccctcctt tatattaagt ttaacaaccc ttttctttct
     5041 tgtctcgtga aaactatgta aatgttctcc atcatagaga gaatagattt ttacgaacta
     5101 agaatatcta cagcaacctt acgaaaatga aagactgtct ccctaattct tctgttagac
     5161 tgtttttaat aaggaaagga tgggaaattt tttgaaaaaa caactactaa ttatgtgcct
     5221 ttcagcatgt gcaattttac tattctcagg ttgtagcaag tccaatgaaa cgattgcaac
     5281 gtttaaaggt ggaacactta cagttcagga tttttatgat aacccaaaag ttaaatcaaa
     5341 taatcaaaca ctattaaaaa aaatgattgt cttcaaagca tttgatgatg tatacggaaa
     5401 agagatttct tcaaaggaag taacaaaaga atataatgaa caaatcaaaa aactcggtcc
     5461 aaactacaag gaacaattaa aagcagttgg ccaaacagaa gagacatata agaaactctt
     5521 taaacagatg ctggctttcc aatacggttt gaaagccaat gtaaaattaa cagataaaga
     5581 tctagacact gcttggaaag aattctatcc agaagttagt acacagatca ttctattttc
     5641 gactaaagaa gaagcagata aagcaaaaaa agaagcaaat gaaggagaaa atttctccaa
     5701 gcttgttcaa gcttatggaa aaaataaaac attaaaggaa actgatggaa aaatgaattt
     5761 tgattcaacc aatccagaga ttcctactga agtaaaaaaa gcagcattta aactaaaaaa
     5821 cggggaagtt tctgatatca ttcctgtaac agatccaacg acttatcagc aaagctatta
     5881 tctagtaaaa atggtaaaaa aacaggataa aggttctaat aaagacaaat acaaatctga
     5941 gctagaaaaa attgctaccg aagcaaaaac tcttgatacg gaattcatgg ataaaacaat
     6001 ccgtaaagtg atgaaaaaag acaatgtaac tattaaagat ccttatgtaa aaaatatatt
     6061 caaataaaca aatttgaccc aattggggat gtggcaaaat ttgagaagtg atttttggaa
     6121 attccttctt atttttgagg caaaccactt ttgccacaac ttcatcgggg ccttttttta
     6181 tttgtacca
//
LOCUS       DAYYME010000139         6173 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000139 DAYYME010000000
VERSION     DAYYME010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.140"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACHBFW_002440"
     CDS             725..811
                     /locus_tag="ACHBFW_002440"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM9318707.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACHBFW_002441"
     CDS             complement(932..1621)
                     /locus_tag="ACHBFW_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318708.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="ACHBFW_002442"
     CDS             complement(1866..2780)
                     /locus_tag="ACHBFW_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM9318709.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACHBFW_002443"
     CDS             complement(2762..3142)
                     /locus_tag="ACHBFW_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM9318710.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACHBFW_002444"
     CDS             complement(3592..3879)
                     /locus_tag="ACHBFW_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM9318711.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACHBFW_002445"
     CDS             complement(3899..4123)
                     /locus_tag="ACHBFW_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM9318712.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACHBFW_002446"
     CDS             complement(4578..4748)
                     /locus_tag="ACHBFW_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318713.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACHBFW_002447"
     CDS             complement(4763..5296)
                     /locus_tag="ACHBFW_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM9318714.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACHBFW_002448"
     CDS             complement(5317..>6173)
                     /locus_tag="ACHBFW_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM9318715.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAYYME010000140         5779 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000140 DAYYME010000000
VERSION     DAYYME010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.141"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..813
                     /locus_tag="ACHBFW_002449"
     CDS             <1..813
                     /locus_tag="ACHBFW_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFM9318716.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            786..1229
                     /locus_tag="ACHBFW_002450"
     CDS             786..1229
                     /locus_tag="ACHBFW_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318717.1"
                     /translation="MGQYHYKLVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2363..3331
                     /locus_tag="ACHBFW_002451"
     CDS             2363..3331
                     /locus_tag="ACHBFW_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM9318718.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3341..3829
                     /locus_tag="ACHBFW_002452"
     CDS             3341..3829
                     /locus_tag="ACHBFW_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM9318719.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4125..4406
                     /locus_tag="ACHBFW_002453"
     CDS             4125..4406
                     /locus_tag="ACHBFW_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318720.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4408..4824
                     /locus_tag="ACHBFW_002454"
     CDS             4408..4824
                     /locus_tag="ACHBFW_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318721.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
     gene            5438..>5779
                     /locus_tag="ACHBFW_002455"
     CDS             5438..>5779
                     /locus_tag="ACHBFW_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HFM9318722.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2155 a          983 c          943 g         1698 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcccttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tttctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgcgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaaatagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaact cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctcgaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaagc
     1441 cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca aacgatacgt
     1501 ccataggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat ttggctagca
     1561 atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt cgagtgagcg
     1621 aatccaaatt tgcaactctg aagctttgca aatgagtgtg ccggcttccg taaaagtcta
     1681 ggtaacatta gtcgatatat acatagggct ctaatttgcg ttttaaggcg ttttaaacgt
     1741 tctggtatgt aattatacca aagcgacttt aaaacgcctt aaaacgaatt ttagaggttc
     1801 tgtagaacgt tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg ctttaaaaac
     1861 aagataaggg attttatcta gacttgtctt aaaacgccgt agatgagcgc tcagagcttc
     1921 taaaaggact ccttacgaac tgtttttaag caaaggagcc tttaggcgac tgcgcagttt
     1981 aatgtgagag cgtagcgatc acacctttaa agcgaccgta gggagcagat tcccagttta
     2041 ggaaatttcg cgaacctgtt tggagaaaaa aagtaggaaa attttaggcg ggaaattttg
     2101 cccccctctt ttataatctc ttttataacc tctttttaaa cctctttacc gtcggctgta
     2161 tccttactct cccaaggtgt gcagaatctt ttaggggtct ttttgtctgt ttttaggggt
     2221 ctttttgtct gtttttaggg gtctttttgt ctgtttttag gggtcttttt gtctgttttt
     2281 aggggtcttt aattaggggg ttgaccccca ttagaaagct ctttataatt ttgttaagta
     2341 gttaatcttg aaggaggatt ttatggcaaa tgaaattgtg aaataccacc atgaattaaa
     2401 tacgattcct ttacgcaaat ttacgtcagt agaaatgaat ttattcttct ctataatatc
     2461 tagaatgcgt gatgtaggcg ataaaaaagt acaatttaca ttcgaacaat taaaagattt
     2521 aagcaattat aaagcaactg caaatgttcg attcatagat gatttagaaa caacgtatga
     2581 caaattaatg gatttacgtt ttggtagaag aagtgctgac ggtttgcaac gtgaacgatt
     2641 tgttttattt aatcaattta aaatcgatgg aaaagctgat attccttttg ctgaaataca
     2701 agtccatgag aaagccttac ccttacttaa taatcttgaa gaatgggttc gatattctct
     2761 ccaacaattc aatgaattag aaagcagtta ttccaaaacc atgtttagat tattaaaaca
     2821 atacagaacc aaaggaattg ccaatttttc aaaagaagac tttcatgaat tacttgatat
     2881 ccctaaaagt tatagagaaa gtgatataaa taaaaaaatt ataaagccta ttcgccaaga
     2941 actaaccgca atcttcaaag gtttgacaat ccaaaagaga tacggaaaag gacggggaaa
     3001 gcctgtcatt ggctatcaat ttacatttaa acctgagata aaaaaatcag acgactttaa
     3061 caaacatgct ccaaccaaag aagaacaaaa taaagctttg gaactagcta atcaacaggt
     3121 tcaatctgaa aaagaaccta cttcattaca agaaaaaatc aaagaacaaa aacgaaaatt
     3181 agaactttta caacagttag cagaattaga aagaaaacaa aataccccaa gtaagaaaat
     3241 taacaaacaa atagcagata acaattccga tattacccca gaatttaaag aaaacttact
     3301 agataatctc caaaacttat ttaaagaata aggggttttt atgacagata aaactatcaa
     3361 agaattagca gatattctag gtgtttcaaa agataaagtg aagtatcaac tgaggaaatt
     3421 acctagtaat tttacctaca aaaaaggaaa tattacctac ctgaaaaaag aagcagtaac
     3481 aacaattaca aaggttttat caggtaatga atcgggtaac ttacctagtg aattacccga
     3541 ttcattacct accgattacc tactcaagga gattgaaact aagaataatc atattgaaga
     3601 aaaagatagg caaattgaaa aacttcaaat agctttagaa acgcagcaaa aattactcga
     3661 ccagcaacaa caactcaccc tacaagccaa tcaacaaatc aaaaaacttc acgaacaact
     3721 ccagctaacc tatgcagaag aatcttcaga aaaagaaccc ctttctgtta ctgaagaaac
     3781 tcaaaaagga caaaacaagg agaaaaaatg gtggcaattc tggcaataac ccaacaattt
     3841 ttattcataa aaattgttgg gttactttct ttttttgctt ttacaagcat agcgacatga
     3901 tctcacgtcg ctatttttta aagagcataa ggaaacgcgg tatcactttc ttacgaaagg
     3961 tgctaggtag gctgtgccta ccacgtttct aagaaaaaaa tttgctatca ctttttcgtc
     4021 attgtggata gcttatgtat agtgtttcgg caaaaaacag ctatacaaat actatatttg
     4081 tatgatatac ataaattata cacaaatgcg aaaggaattc ttctatggac attttagttc
     4141 ggaatattga tccaaaagtt atttcagaaa tagatagacg ttgccaagaa ctcacaacaa
     4201 aaactggcaa aaaatggagc cgtaatgcgt acctaaaagt tctaatcgaa aacgattttg
     4261 accaggcact taggcattac aaaaaagatc aattcgattt attactcgaa aaatttatcg
     4321 atatacaagc aaaaaatact gaaacattga acgactatgt tcatacaaac aaccaactca
     4381 ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa acacgttacc
     4441 ctttatccca gtcacattga cgttttagat tccatcatcg aaaaaaatcc agatatcaat
     4501 aactactcgg aagccattcg ttttctatgt atgagttatg aacctaatac taatacgaca
     4561 actaccagca accaagcaaa actaaatgcg atgggaaaag aaatttctat tatgtccgaa
     4621 atcattgcag aatttgctga tttatatcta aaagatactg gtgttcttat cggtacaaat
     4681 tctaaagtat atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc ctcccgacga
     4741 aatcaaccca aaaatcgtca aaaagaaacc aatcaaacat tcacttctag aaattttgac
     4801 ccaacaaact ttctaaaaag atgaaaaaga aaccgaaaat tttttctatt tctcagtttc
     4861 ttttttaggg ggcagtcttc cagcaagatt tgcaagttac cggtaacttg cacaaaaaaa
     4921 gaaaaatccg cttgtgcgaa tccttctttt tgcattttgg ggcgctgccc caatctccaa
     4981 atatcggtta tttaatagcg tggtaactca aaaaaataga acgtactctg aaataaaaaa
     5041 agactgctct aacgagcagc caataaaaaa taatgagcgt ttattcttga aaaagcagcg
     5101 cgcttatgaa gcgctgccaa aaatttacca tattttatga aaaataacaa gtttctaaaa
     5161 aggaatcggt taagattcac aaagaatcgt aaacagacaa tagcggaaaa tcaagcttcc
     5221 gaacgtacct tttagctaga ccaatgaaaa atcacaaagt gagcggctgg atcccttggt
     5281 acaggcgtta cttggtagta tacacattat gttaactaac gctataaata acgattatgt
     5341 tcccaaaaca atattttatg aactataaca aaaatttgtt atcgcatatt gtatatttta
     5401 attataggtg tataccactt aaatttaaca caaaggcgtg attattttga aacccaaaaa
     5461 gaaaatttat ctaggtctcc tcttttcctt gatgattgga atttttagta taggaattac
     5521 aaatacctat gcttcagaaa attctaattt caatatcaac tcccaaccaa ttgaagaaca
     5581 agcaattaat tttgcgaaaa ctgattttga acactccata acttcaattc ttttaatgga
     5641 aaataacacc gaaactgata ttaactatac tcttggttcg ccatttaaaa tgctcgatgg
     5701 agaaaatacg gcaaatagtt ctattttctt tccagtattt tcagacgctg ataatacgat
     5761 taaatatatt tataccgtc
//
LOCUS       DAYYME010000141         5718 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000141 DAYYME010000000
VERSION     DAYYME010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.142"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            146..859
                     /gene="treR"
                     /locus_tag="ACHBFW_002456"
     CDS             146..859
                     /gene="treR"
                     /locus_tag="ACHBFW_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFM9318723.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            936..1382
                     /gene="msrB"
                     /locus_tag="ACHBFW_002457"
     CDS             936..1382
                     /gene="msrB"
                     /locus_tag="ACHBFW_002457"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFM9318724.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGVFKSLVE"
     gene            1552..2154
                     /gene="ruvA"
                     /locus_tag="ACHBFW_002458"
     CDS             1552..2154
                     /gene="ruvA"
                     /locus_tag="ACHBFW_002458"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFM9318725.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            2167..3168
                     /gene="ruvB"
                     /locus_tag="ACHBFW_002459"
     CDS             2167..3168
                     /gene="ruvB"
                     /locus_tag="ACHBFW_002459"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFM9318726.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            3197..3775
                     /locus_tag="ACHBFW_002460"
     CDS             3197..3775
                     /locus_tag="ACHBFW_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9318727.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            3827..4309
                     /locus_tag="ACHBFW_002461"
     CDS             3827..4309
                     /locus_tag="ACHBFW_002461"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFM9318728.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            4426..4800
                     /locus_tag="ACHBFW_002462"
     CDS             4426..4800
                     /locus_tag="ACHBFW_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318729.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            5230..>5718
                     /locus_tag="ACHBFW_002463"
     CDS             5230..>5718
                     /locus_tag="ACHBFW_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFM9318730.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMD"
BASE COUNT         1985 a          962 c         1213 g         1558 t
ORIGIN      
        1 caagcgtttt attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag
       61 cttttcttaa aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga
      121 tggaagaacg agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga
      181 acagaaaatt ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt
      241 gatcaaaatt tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac
      301 ggaaggttat attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt
      361 cgactttcct atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc
      421 aagtgaaacg atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat
      481 cactggctgg gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg
      541 cgaagtagtt atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc
      601 agcacaagca caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc
      661 ttacgcgcaa aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga
      721 tcttcacgat gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg
      781 ctgtttcgag tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc
      841 ccgacgccga aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt
      901 ggcgctatga taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa
      961 aagaagaact gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa
     1021 cagaacgacc atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata
     1081 tcgtcagcgg tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc
     1141 catcattcag caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg
     1201 gcatgcatcg aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta
     1261 ccgatggacc agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat
     1321 tcattccata tgaaaagatg gatgaattag gttatggggt attcaaatca cttgtagaat
     1381 aatggaaata cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt
     1441 tattcaagcg attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa
     1501 atgtgctaca ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa
     1561 tatctgacag gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt
     1621 atcggctacc agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa
     1681 ataaaaattt atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat
     1741 tcgctagaag aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa
     1801 agtgcccttg caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc
     1861 agtgatgtca cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg
     1921 atcttggatc taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt
     1981 gatgaagcaa acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct
     2041 cttggttaca gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat
     2101 cagacaacgg atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga
     2161 gaccaaatga cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg
     2221 gaaaaatctc ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa
     2281 gaacttcaga tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg
     2341 ttgtacgggc ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg
     2401 accgtaaata ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca
     2461 atcttgaatg aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct
     2521 agagtggtag aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta
     2581 ggacaagggc caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc
     2641 gcgacgacac gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct
     2701 catatggaat attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt
     2761 tttcaaacag agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg
     2821 cctagaatcg ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac
     2881 gggaaaatcg atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct
     2941 ggattggatt atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga
     3001 ccagtagggt taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat
     3061 atgtatgaac cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt
     3121 gctactgctt atgcctatga acattttggt tacgactatc atatatgatt tttgctttta
     3181 gattggagga aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt
     3241 gattgatttg tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga
     3301 agcaaaaatc aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg
     3361 ttgggtctat tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa
     3421 ttgggaagag caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc
     3481 aaatactgtg ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa
     3541 atcatttatc aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa
     3601 actattttat gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac
     3661 gaatgaatat gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga
     3721 tacggaatta tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa
     3781 aaattttttg ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt
     3841 atttgtttgt ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg
     3901 aatagcatta gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta
     3961 tgaagttgga aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga
     4021 tatgacgaat atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat
     4081 cattggcatg gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca
     4141 ggggaaaatc catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga
     4201 cccatactat accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca
     4261 ttggtggaat atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt
     4321 tctttttaag gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa
     4381 aattaattaa gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca
     4441 gtatcgccaa tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat
     4501 tcttttgtaa atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt
     4561 cttgagctgc aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat
     4621 cgtttgagta gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa
     4681 gaccgagtga tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa
     4741 caaattgaaa aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa
     4801 tttataccag atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat
     4861 aaccgaatgc tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca
     4921 tgacagcatt cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa
     4981 aggtcctgct agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag
     5041 ggttgtttgt tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt
     5101 tttcgaaagt tgttaagttt ttgagagtat aaaatatttt gtgtaaatga aaaaatccat
     5161 acaaaaaagg aagtcccttc tgtagaataa agttaacgac aaccaattca cagaaaagag
     5221 gacttcccta tgaatgattt tactacagaa attgtgcaaa ctctagtcac taaaggcgat
     5281 ttaaatgaat tattccgttc gcacttagaa aaagcgataa acacactcct acggactgaa
     5341 ttaacggctt ttttagatta cgaaaaatat gatcgcactg gttttaattc aggtaattcg
     5401 agaaacggtt cttactttcg atcaatcaaa accgaatatg gtgaattaac attggaaata
     5461 cctagagatc gtaatggtga gtttaaacaa caaactttac cagcctacaa aagaacaaac
     5521 gatacattgg aaaccactat tatccattta ttcgaaaaag gtgttacgat gtctgaaatt
     5581 gctgatttga tcgaaaaaat gtacggtcat cactatactc cacaaaccat gtccaacatg
     5641 actaaagttc tgactgaaga agtaaatgcc tttaaatcca gagccttaaa tgataagtat
     5701 gtcgctattt ttatggac
//
LOCUS       DAYYME010000142         5706 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000142 DAYYME010000000
VERSION     DAYYME010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.143"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..1040
                     /locus_tag="ACHBFW_002464"
     CDS             144..1040
                     /locus_tag="ACHBFW_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM9318731.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1190..2131)
                     /locus_tag="ACHBFW_002465"
     CDS             complement(1190..2131)
                     /locus_tag="ACHBFW_002465"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM9318732.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2257..3378
                     /locus_tag="ACHBFW_002466"
     CDS             2257..3378
                     /locus_tag="ACHBFW_002466"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM9318733.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3636..5138
                     /gene="eat(A)"
                     /locus_tag="ACHBFW_002467"
     CDS             3636..5138
                     /gene="eat(A)"
                     /locus_tag="ACHBFW_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM9318734.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5205..>5706
                     /locus_tag="ACHBFW_002468"
     CDS             5205..>5706
                     /locus_tag="ACHBFW_002468"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9318735.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVD"
BASE COUNT         1873 a         1015 c         1204 g         1614 t
ORIGIN      
        1 ttttaataga agaatgattt atttcatcat attttcatac aaatgttaaa ttttcataca
       61 aatataggaa acgttttact ggctttttac tatttaaaca ggctatcata ggaaaaagtg
      121 acagagaaag tgaggcggaa aagatgaaca aaaatgacgt actgctagtt gcaaaaaata
      181 aatcacttta ttttttgcct tccatccgct ctttagatgt ttggtccgga aaagatttac
      241 tacagtcgga attcattaaa tcagctagac aacagttggc aaattatcag ctgatcgttt
      301 ttttagatta tggatttgat atggagatgg ctcgacgcgt gcgcccattt accaatgcaa
      361 aaattgtgtt atttttttgg aatcacttca aagcagaaca cgaggcgatg ctgaagcagg
      421 cacaagatga accggcaatc gatgagattt atcattttga ttttttggaa gcaaaagaac
      481 ttgggctgaa acataatagt tcattttatt cgaaacatat gaatttttct tctagcgaac
      541 caaagacgga tctatttttt ggtgcaacga ataatgggag aaaagaacga gcagaaagct
      601 ataagcagga atttataaaa agagatgtta cgaccaacta tttcattctt ccacatcgag
      661 ggaatgaaca acctggatat cttacttatg aagagtacct gaaaaaaaca ggggaatcgc
      721 gcgggattct ggaactgttg cgggagggtc agcaaggagt gactttgcgg acatttgaat
      781 ccatgtattt tcaaaagaaa ctagtgacgg ataatcaagc gattgctcat tatcattttt
      841 atcgtcctga aaatattttt ttgcttcgag aacgtgattt agatgaatta ccggaatttc
      901 tgcatacacc atttaagcca atcctatcag agctattgga attttatgat gcagaaaatt
      961 gggcaaaacg atttatcact aacgattcca ctatttatga gcaatacgaa tatgacctct
     1021 cctctgttga aaacaaataa aaaacctaca ttttgaagag attatgtact gaaacaaaca
     1081 agcacaattc ttatggcatc atgagagttg tgttttcttt ttgatctttt attgggctta
     1141 aaaaaagaga accagctgct gattcagctg attctcatca gagagccttt tattctgcgc
     1201 caaggattcc ttcttttgca tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa
     1261 tatcatattt ttgtcctttt cgccaaatgg caccattgat gtagtcgatt tcagtcaaac
     1321 gatggttttt gatcaggtct tggtacatag atgggtaatg caacccgatg ttgtcaggat
     1381 cataagtaga agcaatatgt tggtacactt cttcttgatc tagtatgatt ccttcttttg
     1441 ctgcgataga agcaaattca gatacgattg ttcgaaccat gctctctgcc gctttttgcg
     1501 cgcctagttc agcaatatta caatccaaaa tcgtgcataa accattcaat gttccattta
     1561 cacaagcttt tcgccaaatg gaataacgaa cgttatggct atagatcgga tttaaatttg
     1621 cttcttgaaa gacttctaca actttttttg taaatgcttc gccttctggt tccaagttct
     1681 ctagttcgat ttctccatca cctaacaatg tgacttttcc gggaccagct agaccagctg
     1741 tccacatcgt gatcccaaat aagatatttt tctttggcac atatttttcc agtacatctt
     1801 catgacctaa tccatttagt aaacacaaaa cataagtatt gtcagtaata atggattgga
     1861 tcgaacaaaa catatcatct aattgattgg cttttgttaa tgctacgatc aagtccacgt
     1921 gttcgttgct ttcgatgatt tcttctggtg aatagattgg cagttttgcg accacttctt
     1981 taccgttaaa gtcagcaatc aacccatttg tacgaatggc ttcaatgtga gcaggccaac
     2041 gatcgatcaa gaggacctca tttccgcttt gatgtagcat caatccgatt cggctgccca
     2101 ttgctccggc cccagctatt gcgattttca tgttttttct ctcctttatt caatgagttc
     2161 ccttttagta tatagaaaaa tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa
     2221 aaagtgtctt acttaagaag aaagtgaggg atacagatgg atattttggc ggcaattgat
     2281 tcatttaaag gaagtgcttc ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa
     2341 gatcgtaaag gaagaaaaat caatgtgccg attgcagatg gcggagaaaa tactgcagaa
     2401 gcaatctatt acgcgctcag gggaacatgg cgtaccattg atgggatgga tctcttattc
     2461 cgtcagcgaa aaatcagcta tttattgaca acttggcaag gaaaaaacac cgcagtcatc
     2521 gaatctgcac aagcattggg gttagatcta cttgataaac caacggatca aactatccga
     2581 cttgcttcca gttatgcctt aggagaactt attaaagatg cactgcttca agaagtagaa
     2641 caaattattg tcactttagg aggaagcggg tgtagcgatg gtggtctagg attactccaa
     2701 tccttgggtg cttctttaga agggtacaca gaaggaaatc ctttattatc tactaaaaaa
     2761 cttcattttg atcgaagaaa cgttcttgtt tctaaagtaa aattgattat cgctgcagat
     2821 gttaccaatc catatacagg tcaacaagga gctgcttggc aatttggaag acaaaaagga
     2881 ggaatagata cgacgctagc tttcttagat aaacaagcag gaaagattgc tcagcaaata
     2941 aaagatttct gggaggtgga tttagatgat attccaggaa gtggagcagc aggcggtatt
     3001 ggtggagccc tttttttgtt gggagcgaag atggtttctg gtttcgattt agtcagccaa
     3061 atcactggat tggaaaaaaa gatcgaacaa gcagatgtca tcattacagg agaagggcgg
     3121 atcgatcagc aaacgatcca cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa
     3181 tatcgaaaac caataatcgc tatttgcgga agtagagacc aagagctaga aggactttct
     3241 gattttctcc caattgtctt aagtattcat ttgggtccaa taggtttaga agaagcaatg
     3301 gaacacaggc aaacattaga aaaaacaaaa atcttaggac agacacttag cagatttttc
     3361 accatcctcc cgaagtagtt gacaaaatca ttttctttat gctatcgtaa tttcaaatat
     3421 aggacaaagt gagggatccg tgcattgaaa aattaagtcg attgatgaaa gctgcattca
     3481 gactgtacct tgtggagaca aggaagcagt gtgcagattt tcgggattgg cttggttttt
     3541 tcgatgtatg ggtctttttg tgtgtatcca acccttgcat cctctttagc cagttctgaa
     3601 aataggactg gtcttttttt acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc
     3661 tgacattcgg ctacgacagc caaggcacat tattatttga acaagcaaat ctaaattttg
     3721 acacacaatg gaaactagga cttatcggac gaaacggtcg aggaaagaca actttactga
     3781 atattctaca aaacaaacta ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct
     3841 atttcccgca acagacaaaa gataaagaac gtttaaccta ttacgtgtta aatgatatta
     3901 cggattttga gatatgggaa atcgaaagag agctccaatt gatgcaaaca gatcctgaaa
     3961 tcttatggag agaattcagc acactatcgg ggggagagaa gacaaaagtc ctactggcac
     4021 ttttatttgt ggatgacact catttcccgt taatcgatga accaacgaat catttggata
     4081 tctctggtag aaaacaagta gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg
     4141 tcagccatga ccggggattt atcgatgaag tagtggacca tgttttagca atcgaaaaaa
     4201 gtcaactgga actttatcaa gggaatttct ctatctatga agaacagaaa aaacttcgtg
     4261 atgaatttga aatggctcaa aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa
     4321 cagcagctga aaaagccgaa tggtctcgtt cccgagaagg agataaaaca aagaaacaag
     4381 tcggattcat cgatactgaa tctagacgag tgaataaagg agcagtgggt gctgatgctg
     4441 cacggacgat gaaacgatcc aaagcaatcg tgaatcggat ggagacccag atcagcgaga
     4501 aagaaaaact attaaaagat atcgaatata tcgattcttt gacgatgaat agccaagcgt
     4561 ctcaccataa gcgactttta agcgtagaag atcttcaatt agggtatgaa aatctgttat
     4621 tcgagccaat tcattttaca atcgagcctc atcagcgggt ggcgatttca ggtcctaacg
     4681 gtgcaggaaa gtcatccatt atccattatc ttctgggggc attcaacggc aaggttatag
     4741 gagaaaaata ccagccaaaa catctgagca ttagttatgc aagccaaaat tatgaagaca
     4801 atcgaggaac gttggcggaa tttgcagaga aaaaccaagt agactaccaa gcatttttga
     4861 acaacctccg aaagcttggg atggaaagag atgtttttca taacaagatc gagcagatga
     4921 gtatgggcca acggaaaaaa gtggaattgg ctaaatcttt atcacagcca gctgaactat
     4981 atacatggga tgaaccattg aattatttgg atgtcttcaa tcaagaacaa ttagaacaac
     5041 tgatcttgaa cgtgaaacct gccatgttac tagtggaaca tgatcaaacc ttcctggata
     5101 aagtatctac tgagattatt tctcttgaga gaatctaacc cttgtaatct caagaacaac
     5161 aaggtatggt taaggggaac tttgccaacg aaggagagaa tcacatgctt tatgtaacga
     5221 gacacggaga aacaacttgg aacgcacaag gattagtttg cggacgggca gatgtgccgc
     5281 tgactgaaaa aggacagatg caggcacaga aactagcaga aaaggtagta gatttgcctg
     5341 ttccaatcac gaaaatcatc cattcccctc ttcaacgagc aagagataca gcacaagcag
     5401 ttgctgatcg tctcagcttg ccgctgaccg ttgatgaacg attggtagaa atggattttg
     5461 gggattatga tggactgcca agcaaagatg agaactttca aaaagcacgt ttagcgttcg
     5521 ctgtacgatt cccaaatggg gagtcagtct tagatgtgta tgcacggatc gttcctttat
     5581 tgaaagaatg catcgaagac gaagaaaatg tctatctgtt agtttgccat aatgccttga
     5641 taagagtgat caatgcttat tttcatccga tgcctaatga ggggttcttt acattcatgg
     5701 tagata
//
LOCUS       DAYYME010000143         5682 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000143 DAYYME010000000
VERSION     DAYYME010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.144"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            196..921
                     /locus_tag="ACHBFW_002469"
     CDS             196..921
                     /locus_tag="ACHBFW_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318736.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGFSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="ACHBFW_002470"
     CDS             911..1420
                     /locus_tag="ACHBFW_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318737.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="ACHBFW_002471"
     CDS             complement(1490..2938)
                     /locus_tag="ACHBFW_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9318738.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="ACHBFW_002472"
     CDS             complement(2938..3654)
                     /locus_tag="ACHBFW_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFM9318739.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="ACHBFW_002473"
     CDS             complement(3635..3985)
                     /locus_tag="ACHBFW_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM9318740.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="ACHBFW_002474"
     CDS             4129..4902
                     /locus_tag="ACHBFW_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9318741.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="ACHBFW_002475"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="ACHBFW_002475"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(5485..>5682)
                     /locus_tag="ACHBFW_002476"
     CDS             complement(5485..>5682)
                     /locus_tag="ACHBFW_002476"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318742.1"
                     /translation="FPSISIKVHLKKSVRTKVLFVNLKIDFPEYVLGISKCLNVFLNA
                     KSMQKILYFSTFLSLTKIKKR"
BASE COUNT         1647 a         1117 c          989 g         1929 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tttttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aa
//
LOCUS       DAYYME010000144         5654 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000144 DAYYME010000000
VERSION     DAYYME010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.145"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            222..947
                     /locus_tag="ACHBFW_002477"
     CDS             222..947
                     /locus_tag="ACHBFW_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HFM9318743.1"
                     /translation="MNKKRFKRIERKFALTYDQAKYLKTLFPKQVVRDKYSDHFISSL
                     YFDTNDHHFLKEITTEKSYREKFRIRKYGDPQDISPIYLEIKRKFQGMTDKRRIKLAH
                     HELDDMLENIELAETTFSNLEEEYIFNEIQWLFRRLALEPKVYIYYLREAFVNRDSED
                     LRITFDQNIHYRLMNLTTWEHCTEDNLVAPEIDVLMEVKTTGEIPEWLQVGLDRMGKK
                     EIKFSKYKQVYKRYIQEDPLYVQ"
     gene            937..1611
                     /locus_tag="ACHBFW_002478"
     CDS             937..1611
                     /locus_tag="ACHBFW_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HFM9318744.1"
                     /translation="MFNSVLKNEPSQLTLDDFFLCMVVSILLGILIAYTHMYQNNCTK
                     NFMFALIGLPTLVHTVILLINGNLGTGIAVLGAFNLVRFRSAQGNSRDIFSIFWATSI
                     GLATGTGYIVYALFVSSIVAAIQVIIFTLPIKNKHTVFNTEKTIKLKVSKSLFLSSII
                     EEKIAEYTENCNLKKVKINKETCSLHYIVKVKTSMLEKELVRTIMDHPEVISITVGMD
                     KSNKLL"
     gene            complement(1899..2183)
                     /locus_tag="ACHBFW_002479"
     CDS             complement(1899..2183)
                     /locus_tag="ACHBFW_002479"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HFM9318745.1"
                     /translation="MRQKCFLLLSHSQKVKPIFWLSIWDGFFKVSFHFPEKTREKLVT
                     LSLSEGTKEKIKTLQANGNKRKHLSVIFDVDHLEQLADIYQLAEFKKQNG"
     gene            complement(2477..3784)
                     /locus_tag="ACHBFW_002480"
     CDS             complement(2477..3784)
                     /locus_tag="ACHBFW_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFM9318746.1"
                     /translation="MFKKNTRFIVLLASLILCITSFLPSATVQADDAFKINAKAAFAV
                     DAESGKILYDQDGEKTMGIASITKIIGLYIVLEQIKEGKLSWEDKVAISDYAEKLSVV
                     PDLSNVPLHKENDYTVKELVDSAFIQSANASMVALAEKVAGSESKFLELMKKQLKDWG
                     IKDATIVNASGLNNSYLGENRPENTGETDENQLSAKDVAIVARHLILDYPEVLKITST
                     TNQTFGENTQSPVEMVNWNWMLPGFVNYHEGVDGLKTGTTDFAGACFVGTINKDGRRI
                     ITVVLNADNHEENPSARFNETSRLMDYCYDNWSEKELGKANASIPSLKDIKVKDGKET
                     SVNVALKSPVKVWVENGMDTGKLTITPTIDKKKVTDDQVTAPINKGTTVGSATIQLAD
                     DKLGYLEANQEPSTDIITTSTVEKANIFVIGWRHVTDFFSNLF"
     gene            4273..5379
                     /locus_tag="ACHBFW_002481"
     CDS             4273..5379
                     /locus_tag="ACHBFW_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318747.1"
                     /translation="MAKKEKELLSQLTSEGTKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKITSLLDDPSFWRSATTSVAVIVSPEQVFIHRLGI
                     PVDDQYYVSDLPYLLAIIKNGHFNYSYYLLGLNRDSMKLYEVTNKQVKEVALPEDAPR
                     DVVTALGSELTGGGLNYASNGSEGTTFHGVNPKDEEVEIDWVNYYQAVDDYLKDTFEN
                     PEKWPIFLFALPENQTMFKKVAKNPYYRSDVAISASPAQLSLKEIEAGAKKLSQELAV
                     KEASDYNKLLDKKFIDQLVDIIPAAKEGKISHLFIATSNLADGFGEDPETEYDRRQVL
                     NELADNVITNGGQVFILEQKDAPDEKSLVAILRY"
BASE COUNT         1848 a          974 c         1079 g         1753 t
ORIGIN      
        1 cataataaaa tgttaacggc aacaaaatct gtggaaggaa tgaaccggat gatttgatgg
       61 aaaaaacaag atagacaaga agtatacata gtaaaacgtg gaacaagtag gccagaatga
      121 aaaatcagtt tttatccact cgcttttcta tacattcact gtcacaaata taatcgaaag
      181 taattctatt ttgagataaa gtattttata ggagatagta tatgaacaaa aaaagattta
      241 aacgtattga aagaaaattt gcattaactt atgaccaagc taagtatttg aaaacactat
      301 ttccaaaaca ggtagttcgg gataaatata gtgatcattt tatttcttcc ttgtattttg
      361 atacaaacga tcatcacttt ttaaaagaaa tcactacaga aaagtcctat cgagaaaaat
      421 ttagaatcag aaaatatgga gatcctcagg acatatcgcc tatctactta gagataaaaa
      481 gaaagtttca aggaatgaca gataaacgta gaataaaatt agcgcatcat gagctcgatg
      541 atatgttgga aaatatcgaa ttggcagaaa ccacattcag taatttagaa gaagagtata
      601 tttttaatga aatacagtgg ttgttcagaa ggttggcatt ggagcccaaa gtatatatct
      661 attatttaag agaagcattt gtaaatagag attcagaaga tttgcgaatc acttttgatc
      721 aaaatatcca ttaccgtcta atgaacttaa cgacctggga acactgtaca gaagacaatc
      781 tggtagcgcc agaaatcgat gtgctgatgg aagtaaaaac gacaggagaa atacctgaat
      841 ggttacaggt aggattggat aggatgggta aaaaagagat aaaattttct aaatataaac
      901 aagtctataa aagatatatc caagaggacc cgctctatgt tcaatagtgt cttaaaaaat
      961 gagccttctc aactcacact agatgatttc tttttatgta tggtggtgtc gattttgcta
     1021 ggcattctga ttgcttacac ccatatgtat caaaacaact gtacaaaaaa cttcatgttt
     1081 gccttgattg ggttacctac tttagtacat accgtcattt tgttgatcaa cggtaactta
     1141 ggcacgggga tcgctgttct aggcgcattt aacttagtgc gatttcggtc tgctcaaggg
     1201 aattcaagag atattttcag tattttttgg gcaacgtcga ttggtttagc cacagggacg
     1261 ggatatattg tctatgcact attcgtgtct agtatagttg ctgctatcca agtcatcatc
     1321 ttcacgcttc caatcaaaaa taaacataca gtttttaata ctgaaaaaac gatcaagcta
     1381 aaagtttcta aatccttgtt tctttcgagt attattgaag aaaaaattgc agaatataca
     1441 gaaaactgca atttaaaaaa agtaaaaatc aataaagaaa cttgttcctt acactatata
     1501 gtaaaagtaa aaacgagtat gcttgaaaaa gaattggttc gaacgatcat ggatcatcca
     1561 gaagtcattt cgatcactgt aggtatggat aaatcgaata aattattgtg ataaccaaga
     1621 aagtacttaa caatgaagta agaccccagt tccaaagttt agcggggaca cttcaataaa
     1681 gtactttctt ttttgtcttc agcaactact ggtaaaactg agagcaagct ttcgtgagag
     1741 tacagcgata aagatcaacc tcccttttta tctcgagcaa tttccttgca aggctgtttc
     1801 gttgatctta aacgagttag caacagtcaa agatgcgatg gccataaaaa aattgcatta
     1861 agttggctgg tatctgatag ctgggaatct aaggaagact atccattctg tttcttaaat
     1921 tccgctagtt gatagatatc agccaattgt tccagatgat caacatcaaa gataaccgaa
     1981 agatgtttcc ttttattccc atttgcttga agagttttta tcttctcttt ggttccttca
     2041 ctcaatgaga gtgtgactaa tttctccctc gttttttctg ggaaatgaaa ggatactttg
     2101 aaaaatccgt cccaaataga aagccagaag atcggtttta ctttctgaga gtgggacaaa
     2161 agtaagaaac acttttgtct cattctttaa aaacgaataa acggtgggag cagaagcaac
     2221 tccttcggaa ataagccaaa attcacaaaa atttgaaaag caattttcgt gaatttcttc
     2281 ttatttctcg gagttaaaca cttctgtccc aacctcttgt attcaccttt tgatagccaa
     2341 gcttttccat cattataaaa ccgcaaattt caaaaaaaaa cgaacaatac aaaaagcgta
     2401 ttgctcgttt ttctcttcag tttctcacac ctaaacgact tttgtctcga cctctttttg
     2461 gtgactattc atgatgttaa aataaattac tgaaaaaatc tgtgacatgg cgccaaccaa
     2521 tgacaaaaat attagctttt tcaacagtag aagtggtaat gatgtctgtc gaaggctctt
     2581 ggtttgcttc caaatagcct aatttgtcat cagctaattg gatcgttgct gagccaactg
     2641 tagtgccttt gttgatagga gcggtcactt ggtcatccgt tactttcttt ttatcaattg
     2701 ttggagtaat cgttaatttt ccagtatcca ttccattttc gacccagact tttacagggc
     2761 tttttagcgc aacattgacg gaggtttctt ttccatcttt cactttgatg tcttttaggc
     2821 ttgggatgct tgcatttgct tttccaagtt ctttttctga ccagttgtca taacaataat
     2881 ccatcaatcg tgatgtttca ttgaatcggg cacttggatt ttcttcatgg ttatctgcat
     2941 ttaaaacgac tgtgatgatt cgtcggccat ctttgttgat cgttccgaca aagcatgcac
     3001 ccgcaaaatc agttgtgcca gtttttaatc catcgacacc ttcgtgatag tttacaaaac
     3061 caggtagcat ccagttccaa ttgaccattt caaccggtga ctgtgtgttt tcgccgaatg
     3121 tttgatttgt ggtactcgtg atttttaaga cttcaggata atctaagatg aggtgacgag
     3181 caacaatagc gacatctttc gcagacagtt gattttcatc tgtttcacca gtattctcag
     3241 gtcgattctc acctaggtaa gaattgttta aacctgatgc attgacgatc gttgcatctt
     3301 tgatacccca atcttttagt tgtttcttca ttagctcaag aaatttactt tcactgcctg
     3361 caactttttc agctaaagca accatggaag cattggctga ttggatgaaa gctgagtcaa
     3421 caagttcttt cactgtataa tcattttctt tatgcaaagg aacattcgat agatccggca
     3481 cgacacttaa tttttctgca taatccgaaa tggctacctt atcttcccaa gataatttac
     3541 cctctttgat ttgttcaagt acgatatata gaccaatgat ctttgtgatg gaagcaattc
     3601 ccatcgtttt ttcgccatcc tgatcataaa ggatttttcc actttctgca tctacggcaa
     3661 aggcagcttt ggcatttatt ttaaatgcgt cgtctgcttg aacagttgcg ctaggcaaga
     3721 aagaagtgat acataagata agacttgcta atagtacaat aaaacgggta ttttttttga
     3781 acatctttgt tctcctcatt tttaaattga ttcttacaac ttagaatatt atcagagaga
     3841 acaaaaggtt tcaatggttc tgaaaaaaac cttataaaga acaagtctta ttaatgaaga
     3901 atcatagaaa gttatctttt gggttcattg acacgaactt tgactggctg aacttcgggt
     3961 tcggttaggc gtgatgctgc aaaaagggcg cctgctgaga gtgcgaccaa actaaaaatc
     4021 atgaataatt gcataagatc acctcgttct tttttgtttt tttatagtat agcaagagag
     4081 tttgaagcta ctgttaagga aatgagcata taattgatga aagtatgaaa aaaatatgaa
     4141 gtaggacaac acaactaaaa atcgatcagg tatttctctg gtagggattc gttttattgt
     4201 attgataaaa tgggatgtgg tagactttat ttacagatac aaagaaatga tttttttagg
     4261 aggggtacgt ttatggcaaa aaaagaaaaa gaattattat cacaattgac ttcagaaggt
     4321 accaaaggac cgtttgtcac gattatgtta aacacccatg ttgcgcatca agacgtagaa
     4381 aaagatcaaa tcaaattcaa gaattttgca aaggaagcga aaaagcgctt tgaaaagaaa
     4441 tactctgagc atgattggtc tccattccaa gaaaaaatta ctagtttgtt ggacgatccg
     4501 tctttctggc gtagtgccac aacgagtgta gctgtcatag tatcaccaga acaagtgttt
     4561 atccaccgtt tgggtattcc tgtagatgac cagtattatg tgagtgatct accttattta
     4621 ttagcgatca tcaaaaatgg acatttcaac tattcatact atttattagg attgaatcgt
     4681 gattcgatga aattgtacga agtaacgaat aaacaagtca aagaagtggc tttgcctgaa
     4741 gatgcaccaa gagatgtcgt cactgcttta ggatctgaac taacaggagg cgggttgaac
     4801 tacgcatcaa atggtagtga aggtacgacg ttccatggag tcaatcctaa agatgaagaa
     4861 gtcgaaattg attgggtcaa ttattatcaa gcagtcgatg actatttgaa agatacattt
     4921 gaaaatccag agaaatggcc tatcttctta tttgcattac cagaaaatca gacgatgttc
     4981 aaaaaagtgg cgaagaaccc ttattatcgt tccgatgttg cgatctcagc ttcacctgca
     5041 caactttcat taaaagaaat tgaagcaggc gcaaaaaaac tatcgcaaga attagctgtc
     5101 aaagaagcaa gtgactacaa taagttgctt gataaaaagt tcattgatca actcgttgat
     5161 attataccgg cagcaaaaga agggaaaata tcccatttat tcattgctac atctaattta
     5221 gcagatggtt ttggcgaaga tccagaaaca gaatatgacc gccgtcaggt gctgaatgaa
     5281 ctagcagata atgtgatcac gaacggtggt caggtcttta tccttgaaca aaaagatgca
     5341 ccagacgaaa agagtttagt tgcgatctta cgttattgag tgaaaactac caaagtagat
     5401 gaatggtcag gcgttgatag aagtgtatag ctccgagaga aggagtttcc tctgtttcag
     5461 ccgttggatt catcttgagc gagagacaaa agctagcttt tgtctctcgc tcattatttt
     5521 tgtgctttag tcccattggc tttgcgaggg gaagatactt ttctcaaaaa aacgaacctt
     5581 gcagttgaga gggggataga tgcgcatact aatttttatc agttaaaaaa tgatgtggat
     5641 cattagagaa ttcc
//
LOCUS       DAYYME010000145         5599 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000145 DAYYME010000000
VERSION     DAYYME010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.146"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      123..220
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            336..917
                     /locus_tag="ACHBFW_002482"
     CDS             336..917
                     /locus_tag="ACHBFW_002482"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFM9318748.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            920..2227
                     /locus_tag="ACHBFW_002483"
     CDS             920..2227
                     /locus_tag="ACHBFW_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFM9318749.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            2643..3131
                     /gene="purE"
                     /locus_tag="ACHBFW_002484"
     CDS             2643..3131
                     /gene="purE"
                     /locus_tag="ACHBFW_002484"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFM9318750.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            3124..4248
                     /gene="purK"
                     /locus_tag="ACHBFW_002485"
     CDS             3124..4248
                     /gene="purK"
                     /locus_tag="ACHBFW_002485"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFM9318751.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            4261..5556
                     /gene="purB"
                     /locus_tag="ACHBFW_002486"
     CDS             4261..5556
                     /gene="purB"
                     /locus_tag="ACHBFW_002486"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFM9318752.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
BASE COUNT         1740 a          958 c         1302 g         1599 t
ORIGIN      
        1 acgttgtcag tttcagaaag tagtgttaga ctgttttcaa tatttgaaac aacatttttt
       61 attcaaatat ggcaaactgc cttcacgtgc aaaacagttg caagaaaatg aatttacact
      121 attattagtt ttacatacct acttatatat cgtcataata tggatgacag tttctagcca
      181 gtaccgtaaa tgctggacta taagtaaaag attggctatt tcacccttct tttcttatga
      241 taaagtggag ggtgtttttc tttaacagaa acatagtagg tttttgcaca aaagcggtta
      301 tttcggtatt aataaatgat aaaggagcaa ataaagtgaa agaattagtt gaacgtattc
      361 aaaaagacgg acgtatatta ggcgagggcg tattaaaagt cgacagcttc gtcactcacc
      421 aagtagaccc tgtattgatg gaacaaatgg gaaaacggtt tgcagaagta tttaaagaac
      481 aaggaatcac gaaagttgtg accattgaag cttctgggat cgcaccagct ttatttgctg
      541 caagagaatt agatgttcca atgatcttcg ctagaaaagc aaaaagtttg acgatggatg
      601 aagaattatt gacagcttct gtttattctt ttacgaaaca agtgacaagt acgatttcta
      661 tttctcgtaa atttttatct tcagaagaca aagtattgat tatcgacgat ttcttggcaa
      721 acggacaagc ggcaaaagga ttgatcgaat tatgccagca agctggagcg caagtagaag
      781 gaatcgggat cgtcatcgaa aagtctttcc aagatggacg tcagttactt gaagaaatgg
      841 gattgagagt cgtttctctt gcgcgtattg cttccttgac gaatggacaa gttgagtttc
      901 ttgaggagga cgcataatcg tggaaaaaga aacacaaaat ggaaaagctg cggttttagg
      961 gctgcagcat ctgctagcga tgtatgcagg agcagtagcg gtgcctcttt tgatcggtac
     1021 aggactaggc tttaatcaag aacagatgac ttatttgatt tcaattgata tctttatgtg
     1081 tggattagcg acattattgc aattgacagt taaccgattt ttcggaattg gcttgcctgt
     1141 cgttttagga tgtgcgatcc aagcagtagc tccactgatc ttgatcggaa ccaatgaagg
     1201 agtaggagct atctacggat ctattatcgc ttctgggatc tttgttgtgt tagtttctgg
     1261 tgtcttttct aaaattaaaa aactattccc tcctatagta acaggaacgg ttatcaccgt
     1321 tattggtttg actttgattc ctgtagcaat cgagaaaatg ggtggaggta atgcaacagc
     1381 agctgatttt ggtgacaaaa caaatctgct acttgcattt gtgacgattc tcctaattgt
     1441 aggcgtgcaa atgctggcaa aagggttcat tcgttcaatc gcggtattga ttggtttagt
     1501 cggaggaagc atcttagctg ctttcatggg aatgattgat ctgagcgcga ttggagatgc
     1561 gccgttgttc catgttccac aaccctttta ttttggtaaa ccaactttcg acgtatggtc
     1621 gattttacta atgatcatca tttccatggt cagcatggta gaatcaacag gtgtatattt
     1681 tgcacttggt gacattactg ggaaaaatgt tggagaagaa gaattgaaaa agggatatcg
     1741 tgcagaagga cttgccgtta tcttaggcgg tattttcaat acattccctt atactggctt
     1801 ttctcaaaat gtcggattgg ttcaactttc gggaatcaaa acaagaaaac ccatctattt
     1861 ctctgcattc ttcttgattg ttttaggatt attccctaaa atcggtgcgt tagcacaaat
     1921 catccctgag ccggtacttg gtggaggaat gttagtcatg ttcgggatgg tggcagtcca
     1981 aggaatccgt atgctgttgg aagttgattt cagcaatgat aaaaatctat tgattgttgc
     2041 tgtttctgtt ggatttgggc tgggtttcaa tgttacgccg acattattca gtcaaatgcc
     2101 gcaaacattg caaatgttca ctggaaatgg catcgtaatg agtagcatta cggctatcat
     2161 attgaatttg atctttaatg gattgaaaaa agatgaagaa atattggaag aagccttcaa
     2221 gaagtaatag agcaaaagaa aggttgtgaa atcagcgttt tgacctgatt gttagagaaa
     2281 caaacggaaa aatagttcct cgtattttgc cgtttgtttg agctaatacc gagggtcagc
     2341 tgattgaaca ccgttgaagg aagaggtcgt gagcctgccg ttcatctccg agtcacaagg
     2401 aacaatttca aaaaaaagct tctcatgttt gttgaatatt gttccttgtg accgaggagt
     2461 tggcaggaga acaccgttta tccataaaaa ggaatgtgac ctaacttttg tcacattcct
     2521 tttttttatc tttaataaac acgaacaata aaatgttttt ctttgttaac attcgaaatt
     2581 tctttgacat caaaaaaagt gattgttaaa atatgaatgt agaaaaactg ggaggaaaaa
     2641 caatgacagc aaaaatctct gtgattatgg ggagtacttc agattgggaa acaatgaaag
     2701 aagcatgtca aatattggat gaattcggtg tgtcttatga aaaaaaggtc gtttctgctc
     2761 atcgaacccc agatttgatg tttgaatatg cacaaaacgc tcgaaaaaaa ggaatcaaaa
     2821 tcattattgc aggagcaggg ggagccgcgc atctgccggg tatggtcgca tcaaaaacaa
     2881 ctttgccggt tatcggtgta ccggtccaat cacgaacatt aaatggtttg gattcgcttc
     2941 tttcgattgt ccaaatgcct ggaggagtac ctgtagctac tacagcaatc ggaaaagcgg
     3001 gagcaattaa tgctggacta cttgcgatac agatgctttc catgtatgat ttagaattgg
     3061 aggctaaatt ggctgaaaga cgttcaatgt tggctaaaac tgttatagaa agtagtgatc
     3121 aacttggtta aacctttgtt gcctggttca acgatcggga tcgtcggtgg cggtcaatta
     3181 ggtcgtatga tggcgattag tgcaaaagaa atgggcttta aagtaggtgt gctggatcct
     3241 gtgagcgatt gtccagccgc acaagttgca gattggcaca ttgaaagtgt ctatgatgac
     3301 acctttgcgc tgaaagagct tgccagaaga acagatgtca ttacctatga attcgaaaac
     3361 gtcagtgtag aggcgttaaa tgcgatttta cccatgtcat ttattcctca agggacagac
     3421 ttgcttgcga ttacgcaaga ccgcctgtta gaaaaatctt ttttggaaac gaataacatc
     3481 gtgattgctc cttatgcaac gatcgtcagt ccgacggata tccaagatgc gattgacggg
     3541 atcggctatc cttgtgtttt gaaaaccact cgaggcggct atgatggcaa ggggcagtat
     3601 gttttgaaaa gtcgggcgga tctagctcct gccatggacc ttttaagaga aggaacctgt
     3661 gttttagaag catggatccc gtttgaaaaa gagatttcga tcatggtggc aggaaatggt
     3721 caaggagatt tcacgacttt tccggtcgtg gaaaacatcc accacaacaa tattcttcat
     3781 gaaacgattg ctccagccgc gattgatcag gatgtgatcg aggaggctga gcgtattgcc
     3841 cgtgtgattg cagaagcagt gagcctttca ggtgttttag gggtagaaat gtttttaaca
     3901 aaaacaggcg ggctatatgt gaatgaatta gctcctcgtc ctcacaattc tggacattat
     3961 tctattgaag cttgttctat gagtcagttc gatgcccata ttcgaggtat ttgtggttgg
     4021 ccattaggag aaaaagtacg tttgataagc aatgcagtaa tggtcaatgt tttgggagaa
     4081 caactgctgg aaagctatcg cttgattgag aataagccag actggcaatt ccatttctat
     4141 ggaaaaaaac aagcaaaaaa aggtcgcaag atgggacata tcactatctt gacagaagac
     4201 atccagcaaa cgctggaaga aatcaaacaa acgaatattt gggattagag gagaaaaaac
     4261 atgttagaac gttatacacg ccctgaaatg gggaatatct ggacggaaga gaacaaatac
     4321 cgtgcgtggc tcgaagtcga gattttagca gatgaagctt gggcagaact tggtgaaatc
     4381 ccgcaagaag atgtgaaaaa aattcgtgag catgcatcat ttgatatcga tcggatcttg
     4441 gaaatcgaaa aattaacacg ccatgatgtc gttgcgttta cacgagcggt ttctgaaaca
     4501 ttgggagaag aaagaaaatg ggtccattat ggtctaacaa gtacggatgt cgtagatact
     4561 gcttatggct atctcatcaa gcaagcaaat gatattttac gggaagactt gaaacgtttt
     4621 acggaaatca ttggcgaaaa agcaaaagaa cacaaatata cagtgatgat gggtcggaca
     4681 cacggtgtcc atgctgaacc aactactttt ggattgaaac ttgccacatg gtattcagaa
     4741 atgaaacgga acatcgaacg atttgaacat gcggcaaaag gagtagaagc aggaaaaatc
     4801 tcaggagcag tgggaacgtt cgcaaatatc ccgccattta ttgaagagta tgtatgtaaa
     4861 caattgggga tccgtccaca agaaatctct actcaagttc tccctcggga tctacatgct
     4921 gaatatttct cagcgatggc gcttattgct acaagtattg aacgttttgc aacagagata
     4981 cgcggattgc aaaaatccga aacacgtgaa gtagaagaat tttttgcgaa aggtcaaaaa
     5041 ggttcttctg ctatgccgca caaaaggaat ccgatcggtt cagaaaatat ggctggtcta
     5101 gccagagtgg tgcgcggaca catggtcact gcctatgaaa atgtcacatt atggcatgaa
     5161 agagatattt ctcattcttc agcagaacga atcatcttgc ctgataccac gattttattg
     5221 aattacatgt taaatcgttt tggcaatatc gttaaaaatc taacggtctt tcctgaaaac
     5281 atgaagcgga atatgtctgc aacatttggt ttgatttata gccagcgcgt tatgctgaaa
     5341 ttgatcgata agggaatgac aagagaagaa gcttatgatc ttgttcagcc aaaaactgcg
     5401 tacgcttggg acaaccaagt gcagttccgc ccgctgttag aagaagatcc aaccatcaca
     5461 tcggtattat ctaaagaaga aatagacgat gcctttgatt atcatcatca tttgaaaaac
     5521 gttgacgtga tattcaaacg agtaggtttg gaataaagaa gagtgagttg caaaattcaa
     5581 ttaaaataga atagcaaaa
//
LOCUS       DAYYME010000146         5566 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000146 DAYYME010000000
VERSION     DAYYME010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.147"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..955)
                     /locus_tag="ACHBFW_002487"
     CDS             complement(<1..955)
                     /locus_tag="ACHBFW_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM9318753.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDG"
     gene            complement(1026..1322)
                     /locus_tag="ACHBFW_002488"
     CDS             complement(1026..1322)
                     /locus_tag="ACHBFW_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318754.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            complement(1315..1608)
                     /locus_tag="ACHBFW_002489"
     CDS             complement(1315..1608)
                     /locus_tag="ACHBFW_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFM9318755.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1754..2305)
                     /locus_tag="ACHBFW_002490"
     CDS             complement(1754..2305)
                     /locus_tag="ACHBFW_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFM9318756.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2479..2778)
                     /locus_tag="ACHBFW_002491"
     CDS             complement(2479..2778)
                     /locus_tag="ACHBFW_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFM9318757.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            complement(2805..3113)
                     /locus_tag="ACHBFW_002492"
     CDS             complement(2805..3113)
                     /locus_tag="ACHBFW_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM9318758.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            complement(3230..4189)
                     /locus_tag="ACHBFW_002493"
     CDS             complement(3230..4189)
                     /locus_tag="ACHBFW_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9318759.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            4333..5109
                     /locus_tag="ACHBFW_002494"
     CDS             4333..5109
                     /locus_tag="ACHBFW_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9318760.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            complement(5207..>5566)
                     /locus_tag="ACHBFW_002495"
     CDS             complement(5207..>5566)
                     /locus_tag="ACHBFW_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9318761.1"
                     /translation="KKKKIKMAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESK
                     PNPTIFLKASEQLRIPVEECLVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDV
                     IYDIKYLRQEVQVKFGI"
BASE COUNT         1663 a         1029 c          855 g         2019 t
ORIGIN      
        1 ttccatctgt ataaacacgg aattgttttc caccagccat tgttgtattt ccattatcat
       61 ccgttacaat attaattgaa tagtagcttc cattttcatc ttgcattaga tctttcatat
      121 attcatttgt gctaattaaa ttagtatttg atgcattttt atcaggagaa tcatacaacc
      181 ataaagcttt attatttagt acagtttctt tagcagcttc ccaacttgaa attgctgagc
      241 tacttgtaga ttgtgtcgtt gtagttgttg cttgttgagt agattcttct actactggag
      301 tttgaggagt ttgtccattc acaatatcaa tgaaaccttg ttttaactgt tggttctgag
      361 tttcattgaa gtacatgtag tttttatcat tgccttgatt ttgttgcgtt aaataaactt
      421 taggttgtcc attttgaaag ccaaagaaat atacatgctg ccctaaatat tctccagttt
      481 ctacatcaga ataaactgct actaaattga aatctgcatg accagttcca tcttctgacc
      541 attgtacggt aactggtgct tcattgattg ccattttcca atttccatca ataactgcac
      601 ttggtaaagg cgtaccatac agacttacat tgttctgagg attgtagctt ttatattctt
      661 gacctaatgt ctttccccat tgtgggacaa aaattgctag ttgagtagat ttctcactat
      721 cccacaaagt atttttaacc tcttcagaag aagattcaat gattgaactt tcacttgtac
      781 ttgcttccac agattgtttc atttctgttg tagtttgttc agttgattgc gttttacttt
      841 ttttagcctc aattgttgta tcagtttcta cagtctcttt agtcctcgta ttctcagatg
      901 tacaaccagt gaataaaatc cccattccaa ttagtaataa caaccatttt ttcattttat
      961 ttcccccttt caatttctcc ctcgcgtact tattttatca gtcaattaat ctcaatggtg
     1021 ttcatttagt taatcctttc ccaagaatgt cgatagccag catgacaacg atctcttcct
     1081 tgagggcttt cagatggatt aaaactagta gaaatttccc ttccacatcc tctacagcga
     1141 taagttctca ttatttttgg gctattagta ttatttttct tagttagagc tatgagtcca
     1201 actatgatta ccccaactgt cttaattact tttcctttat ctatattcgg atgcgcaagc
     1261 atatatttat ataaccaata tccaccgtaa attaaaccac ctaatacaat cagcttagcc
     1321 atgtactcgt ctcctttctt tttgtttaat tactttttga atccatcttg cagagaaacc
     1381 ataaacttct gtcaattgac ggatatctac agttttttcc tgttctatca tgttttcgat
     1441 tctttttcta actaactcac gatcgtacat tttcattgga aaagaaatct gttgccctcg
     1501 aaaagcaaga taaaattgat agagcaattc tgaatccagt tccatttcct caaaaaagtc
     1561 attcatactt ttataagagt tactcaaaca agtttcatca atctccatct acacaccccc
     1621 tgttattttt tacataatga aaatagcata ttactgggta cattgataat tcatataaca
     1681 tgtatcatca catgtatttt taaagtcaaa aagcatgtaa aactacttat aagtaacata
     1741 agaaaataaa atgttatttt tcttgaaata ataattgact atctgccaat tgccattcat
     1801 tacgaacatg aatacttagt gggtgggata acttaccttc tgcatttatc aatactttgg
     1861 tcatcttttt ttgtttcgca ctcaattgat cgtaaatggt cttcgcttgt tcataagcca
     1921 cttgactcgt cgctgtaatc gaaccacaag caaaaataat ggtgttgatt ttcttttctg
     1981 tgaaaacagt tgttaatatt tccaaagatt gttcatcatt tccacgagga aatacccgag
     2041 gacttttcgc ttttgtatac gaaaaaagat tcacagcaat aaaaccagta aatcctaatt
     2101 gtcggatatt cttttcgatt aataacatac tcaaatcatt cgtaaatggt tctgtatctg
     2161 ttggacgaat agtaatgact accgctaatt tatcttccgc ttcttttcct ttgatcgccc
     2221 attgtcgttt gaaaacatag cgatgggtat gatgaggatc tcgccaaact tctgtttgtg
     2281 tggtcactaa ttctttttca atcatctctt tttctcctat gctaattttt gttcaagcaa
     2341 ttcaatcacc attgccgtta taagtgtcaa tactagttga aaatatttca tattctttct
     2401 ccttttttct tcagaatttg atgaatcgct gtaaggatat cttctaaaca tttcgcaatc
     2461 attctcaaat aatcgatctt attcattcgt tcctccaatc tgactccaaa ccaatcgtcc
     2521 ttctaccagt aacccacgaa caaattctcg ctggctggta atatctgtaa tgaaataaac
     2581 tacatagtct tttccatctt ctggtgcgca ataaatataa agatgtgtca tcactaaacg
     2641 tggtagagta acatcttcag gaagcatacc catttcaatt tcctcttttt ccatcgtaac
     2701 ggattcatag ccccactctt caggagtagt gactagctgt gcacactttt ccagtacttc
     2761 gatagccgtt tctaacaagt cctttcgttc ctttctttta gatatcagta ttcttctggt
     2821 aataacatcg tttgatactc acccgaatca atgacccata ttttttgaga cactgatttc
     2881 aaacgaagct taggtgaaat cagatactct tttcttgctt ctggttcttc ttgacgatga
     2941 accaactttt gatttccttt ttccgtagaa aattcaaaga tttgaagata gtctaaatga
     3001 aattgctttt gaacgttttg atcgattaga ttccaacata aagcttgtaa ttccaccggc
     3061 aaactgtcca ttactccctt tgtgacataa cgttgtcctt tattttgaaa catttctttt
     3121 ccctcctttg ccataaaaat agcccaatct tctattaaag aagattgggc ttcatggata
     3181 taatatatat ttgatattca atttgattca ttttatacta tctgatttgt caaattaagc
     3241 tagacaatat gtctttatct atgcagctca aaaatacttc caatggtgtt cgataattta
     3301 atgattttct tggaatatgg tttcttttcg aggcaacgga ttgaataaag gtttcatccg
     3361 tgtccctaaa gtccattgtt ttaggtaagc catctcgtct aagcaagccg tttgagtgct
     3421 catttagccc tctttgcgaa ggtgttcctg gatctgcgaa aaagatggca atatcgttcg
     3481 agttacttat agatcgccag ttggaaaatt cttttccaca atcaaatgtc atggatttaa
     3541 acaagtgttt aggcatggat ttgaaccaat catttaacct gttttcgata tcttcggctc
     3601 gtctgcctgt cggtttcaaa gtaataataa ccttggataa acactcaacc aatgtaataa
     3661 cagcgctctt gtgtttaact cccacaatgg tgtcgacttc gagatgacca aattctttag
     3721 tcaggtgtgg atagagactg ttccgttcat gaatcgttct gcgaaaggcc tgttttccac
     3781 gcttttcttg atgattattc ggtttacgtt tccccttcat aggtaacgac tgtgtgtcaa
     3841 aaaggccttt tttaaacatc cgatagagtg tacgaacaga acaatcaatt ggaaactcgc
     3901 cacggccgat aatcacatcg ggtgaccatc cttgagctac cttttcttga acatatgttg
     3961 attgttcatc agataaaacg gttgaacgtc gtccacaacg agatttattt tgtttgtatt
     4021 gacggtaata atctagcact ttttttcctt gtttgagaaa cgaataaaca ttataaatgg
     4081 tttgtctaga gcgatttaaa ctttgtgcga caagagcgac aggtttctcg atattgaaat
     4141 aagactctat gattactagt tcgtctgttg taagatgggt gtaagtcatt tgtactcact
     4201 ccttattaat tctttggtcg gaactatttt gagtgtagca caaatgatct ttttagttgt
     4261 ctagcttaat tttacaatcg acgttttaat aaattgtgat ataatgaagt ataacttttt
     4321 agggagtgta ctatgaattt tcaagataat agaccattat atttacaagt agaagcttct
     4381 atacgtaacg atattcgtga aaaaaaatat cttccaggag agcaattacc tacagaagaa
     4441 gaattatgta aaatttatgg agtaagtaag attaccattc gaaaagcttt taaactttta
     4501 gcagaaagtg gaacagttga acgattaagg gggaaaggga cctttgtaac tcaaaaaaaa
     4561 gaaagtttaa aattagctgg agttcatgga tttagcgatt ctctttctac ccgaggacat
     4621 aaaattagat atactgtttt acattctaaa attataaagg ctgatgagtt tttagctaat
     4681 aaattacaaa tcaatgtaac tgatagagtt tacaatatta aacgtttaat gtgggaagac
     4741 ggtgccccga tgggaattga taattttttc gcatcagaag aaaaatttcc aactttattt
     4801 gaaaaaggta gccaatatac ttcattatat aaacttctag aagatgacta tgatgtcaaa
     4861 gctacgagct ctattatgga aataaacggt ttatctgcaa cacctgagct ttccgactta
     4921 ttgcaatgta tagtaggaga tccgctattt attttagaaa aggttggcaa agataaaaat
     4981 aatcaaccat ttcattattc tcaatccaca gttcgttgtg atagaattag ttatgttatt
     5041 gaagtcacag aaaatatcac tttagatacg gtgcataaaa atgattcaga tcctaaagta
     5101 atagattgat attaaaagaa atcataccca aagcaatcaa ttttgattag atttgggcat
     5161 gatttttatt tttactaata ttctctaaat acatcaaaaa acttatttat attccaaatt
     5221 tcacctgtac ttcttgtcgc agatatttaa tatcgtaaat aacatcatct gctagactta
     5281 catcttgaga aaaacgttta tctttaattg caagaacctt catatttgct cttttaccag
     5341 cttttatacc attataagaa tcttctatca ccagacattc ttcaactgga attctgagct
     5401 gttctgaagc tttaaggaat atagtaggat taggtttact ttctggcaaa tctcttccgc
     5461 taattaaaca atcaaaatat gactctattt tacaagtggt aacaacttca tttatagtat
     5521 ccattggact ggaagaagca attgccattt ttattttttt cttttt
//
LOCUS       DAYYME010000147         5533 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000147 DAYYME010000000
VERSION     DAYYME010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.148"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            169..1680
                     /gene="gadC"
                     /locus_tag="ACHBFW_002496"
     CDS             169..1680
                     /gene="gadC"
                     /locus_tag="ACHBFW_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFM9318762.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            1693..3093
                     /locus_tag="ACHBFW_002497"
     CDS             1693..3093
                     /locus_tag="ACHBFW_002497"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFM9318763.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(3258..4693)
                     /locus_tag="ACHBFW_002498"
                     /pseudo
     CDS             complement(3258..4693)
                     /locus_tag="ACHBFW_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            5086..>5533
                     /locus_tag="ACHBFW_002499"
     CDS             5086..>5533
                     /locus_tag="ACHBFW_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFM9318764.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVS"
BASE COUNT         1771 a          890 c         1051 g         1821 t
ORIGIN      
        1 ttcacttttc taaaaatgaa aataaaaaat tctggaagtg gagaagaatt ttgcttgcta
       61 tactttgtct tgtagaaaag aggtagacaa aataatgcaa gctcaaataa gcgattttcc
      121 ataaccttgg taggaatgac aaattaaata ggaaagggaa gtaataatat gaataaaaaa
      181 aagttatctc tattcggttt ttttgccctg acagcttcca tggttttgac tgtttatgag
      241 tatccgacct ttgcaacatc acaattacat ttggttttct ttcttctttt gggaggtatt
      301 ttatggtttc taccagttgc tttatgtgct gcagaaatgg caacagttga aggatggaaa
      361 gatggtggga ttttcagttg ggttagtcaa actttaggag aacgttttgg ttttgcagct
      421 atttttttcc aatggtttca aattacagta ggttttatta caatgatcta cttcatacta
      481 ggagcgctgt cgtatgtaat taattttcca gagcttaata cagatccatt tatcaagttt
      541 attggtgtat tagtcatttt ctgggtactg actttttcac agttgggcgg gaccaatcgt
      601 accgctaaaa ttgctaaagc aggatttgtc attggaattg ttattccttc gatcattctc
      661 tttggtttag cagcagccta ctttatcggc ggtaatccga ttcaaatggc tattaatgcg
      721 aaagctttta ttcctgactt ttctcagctt tcaactttag tagtatttgt atcttttata
      781 ttggcttata tgggcgtaga agcttcagca tcacatatta atgagctgga aaatccccaa
      841 agaaactacc cattggcaat gtttttacta gtgattttgg ccatcgcctt agatgcaatc
      901 ggcggtttct cagtagcagc ggttattcca caacatgact tgtctctcag tgctggtgta
      961 atccaaacat ttgaaacgct tattttacat tttaacagtc atttacattg gttggtaaaa
     1021 gtgattgctc tcatgattgc ctgtggcgtt atgggtgaag ttagttcttg ggtagttggt
     1081 ccatcacgag ggatgtttgc agctgctcag cgaggtctgt taccagtttt cttccggaaa
     1141 actaataaac aagacgttcc agttccgttg attatgatac aaggaattat cgttactatt
     1201 tgggcagcag tcttaacttt tggtggagga ggcaacaatt tatcattctt agtagcaatc
     1261 tccttaactg ttgtcattta tctcgtagga tatttactat ttttcctagg ttactttgta
     1321 ttagttttta agaaaaaaga cttaaaacga acttacaatg taccaggaaa aactgttggc
     1381 aaaacgatta tcggtggaat tggttttatt ttatcagtgt tcgcattgct tatctctttt
     1441 gtaccaccag cttcaattgc agctagtgag caccgaacat accaaatcat actacttatt
     1501 agctttgtag tgactgttct tttacctttt atcatttatg aagtacatga taaaagtgct
     1561 cacacaacta ttgaggaacc tactcacgtg aaagctgaag acgttaatcc cgctgtttat
     1621 ccagctgcta gaggagagca ccacgttaca aaaaatgatg aacatatttt aaaacattag
     1681 gaggaattca ttatgttata cggaaaagat aatcaagaag aaaaaaacta tttggaacca
     1741 atttttggct ctgcaagtga ggatgttgac ttgcctaaat ataagttaaa caaagaatcc
     1801 attgaaccac gaattgctta tcaattagta caagacgaga tgttggatga aggaaatgcg
     1861 cgattaaact tagctacttt ttgtcaaacg tatatggaac ctgaagcagt gaaattgatg
     1921 acccaaacgt tagaaaaaaa tgcaattgat aaatcagaat acccacgaac aacggaaatt
     1981 gaaaaccgct gtgtaaatat gattgctgat ttatggcatg ctccaaataa tgaaaaattc
     2041 atgggaactt caacgatcgg ctcttcagaa gcctgcatgc tgggtggtat ggccatgaaa
     2101 tttgcttggc gtaaacgtgc tgaaaaatta ggtcttgata ttcaagcaaa aaaacctaac
     2161 ctggtgatct cttctggtta ccaagtttgt tgggaaaaat tctgtgtata ttgggatgtg
     2221 gaactgagag aagtcccaat ggatgaaaaa catatgtcaa ttaatctaga tactgtcatg
     2281 gattatgtgg atgagtacac aattggtatt gtaggtatta tgggtattac ttacactggt
     2341 cgttatgatg atatcaaggg tctgaatgat ttagttgaag ctcacaataa acaaactgac
     2401 tataaagtat acattcatgt tgacgctgca tcgggtggct tttatgcacc atttactgaa
     2461 cctgatctag tttgggattt tcaattgaaa aatgttatct caattaattc ttcaggtcac
     2521 aaatatggtt tggtatatcc aggtgtgggt tgggtcttat ggcgtgacca acaatactta
     2581 ccagaagaat tagtatttaa agttagttac ttaggtggag aaatgccaac tatggctatc
     2641 aacttctctc atagtgcagc acaactaatt gggcaatact acaactttgt acgctatggc
     2701 tttgatggtt atcgtgatat tcaccaaaga actcatgatg ttgctgttta tttagccaaa
     2761 gagatcgaaa aaactggtat ttttgaaatc attaatgatg gatcagaatt gccagttgtg
     2821 tgctataagc tgaaagaaga tcccaatcgc gaatggacac tatatgattt atctgatcgt
     2881 ctgttaatga agggatggca agtcccagcc tacccattac ctaaagactt ggatcaatta
     2941 attattcaac gcttagttgt tcgagcagac tttggaatga acatggctgg tgattatgta
     3001 caagatatga accaagcaat tgaagagttg aataaagctc atattgttta tcataaaaaa
     3061 caggatgtga aaacgtacgg ctttactcac taaaattctt cagagaaata taaaagaagt
     3121 taattaacta ataaaaggag ttgtgattac cacacagctc cttaaatcta tttgaataaa
     3181 tatatgatat tcatttgtta cactctcaat cttagctgtt gttctattca tcattactct
     3241 tattgtaagt agttcagtca aatttgcttg ttcaattgat cgataacagt atgaattgtt
     3301 tgaaaagtaa tcggatatat aattgttttc tcactaccta agccttcttg gtataaagaa
     3361 ctatcgcaaa gtataatccg ctgagtacaa aagaaagtgt cgtctactat ttctctaagt
     3421 tgtttacaaa tgatattact aatagaaaag tgttctccca aaatgtcttc taactgttgt
     3481 cgatagtata aatacctaaa gtctgacgga gctactatga acacttcaat tggagatttc
     3541 cgtttatgct catttaaaag acttaaacta atggttagtt tacgaataga attctcattc
     3601 caacgcagaa ataaattgtt ttgttttcgc cattgttcaa gaatttcatg aacttctaag
     3661 tacaaagcgc gttgctcttc atttaacaag taagtcttat caggttgaaa tatctgaata
     3721 tcagcccaaa gtgagcggat aaacgggaga agtgctttat caaaaagtat ctgattttct
     3781 tcagatatat tcagagataa gataagtttt tgactgagct caatcaccaa agggttatct
     3841 gctacatggt tttgataaac aacgttcaag tctttttgta acaattcaaa gttatcgcat
     3901 agataatttc tagagttgaa tagaaagtag atataaaggc attcatcttc gtcatgacat
     3961 aaagagaaag aaagttgctc taatcctttc tgtattagct gaaacaaggg agtatgtcgg
     4021 gcaactcttt tttcttcctg actaaaatat attgggttag tcttagatct ctgcaaaccg
     4081 atagcgatac ctaataaaat aaattgtttt tcaactgtag ggtaacttct tgaaaaaaat
     4141 gtttttctac ataatcaatc aattgttgac cgctttgaat aattgattta tttagctgga
     4201 tcaactctgt ccgatttgtc tcatagatta tcgttagtaa caaacttcga taatcctttt
     4261 caggaatttc aaaatggcca tcagcattta agtactgcat ttgtttaaaa aaattaatca
     4321 actcatttcg taagttgtaa actttgctta acgaaatgaa ggtagtatca gctatattac
     4381 tccaattgtg ttcccctgat aaataatgtc ctagtacatt gaaaaagtca gagcgctgat
     4441 agatgagttg agtgagtgcc aacctcgaaa acgaagagtc gaatgcgact gtaaagctac
     4501 ttctagattt ctccactgaa ataatttgct gatggagtat gtcggtaatg cattccaagt
     4561 cgttagtgac agtgagtggg caaacgttca attgatctgc tatttcgcga atctgaatgg
     4621 tccctttttc gtgtaattgc tcacaaatgt aaacttgacg aaaaatactt ttctccagat
     4681 aggtttccaa catagtactc ctcctagtca ttttatacat aactattata actctaccaa
     4741 tcggaatagt cctgtaatat attgaatcaa aaatataaat tataaaataa ttagatgggg
     4801 tatttggttt aagtatccct tatatagtaa taaatttata ggtgtgattc tcgaattaat
     4861 acccgaaaat gtgagagaat ttttatggat tttttaggat acaaattaat tatttttgct
     4921 tttatcctga ttaccagtcc agtactgtcg gtatcccatt ttgcagcaaa atcatgttga
     4981 taaagtgaca agaagactga tgagatatat tatccaaatt gagaagataa ttaatcggat
     5041 tcttgagtat accctacttt ttaagaaagt agggtataca agcgtatgaa taaagacata
     5101 actttccctg tgatggacgt tcggcttgtt ccaattacca aaatacaggc gaacgattat
     5161 aatcccaatc gagtagcgtc tccggaaatg aacttattgg agctttctat cattgaagat
     5221 ggctttactc aaccattagt ttgttattac aaggaaaaag aagagaagta tatcttggtt
     5281 gatgtgcttc ataggtatcg tgttgctaag gaaagactca acttaaaagt tgtaccggta
     5341 acagttattg ataaacccat agaagaacgt atggcttcaa caattcgaca caatcgtgca
     5401 agagggaccc atgaaataag aggaatggaa attgtcatcc aaagattagt aaaaaatgga
     5461 tggtcagaca agagaatatc caaagaatta ggcatggaca ttgaagaaat ttttcgcttt
     5521 aagcaagtgt ctg
//
LOCUS       DAYYME010000148         5529 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000148 DAYYME010000000
VERSION     DAYYME010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.149"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..1561)
                     /locus_tag="ACHBFW_002500"
     CDS             complement(56..1561)
                     /locus_tag="ACHBFW_002500"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFM9318765.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(1653..2243)
                     /locus_tag="ACHBFW_002501"
     CDS             complement(1653..2243)
                     /locus_tag="ACHBFW_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9318766.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            2422..4068
                     /locus_tag="ACHBFW_002502"
     CDS             2422..4068
                     /locus_tag="ACHBFW_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM9318767.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPRY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            4117..4836
                     /locus_tag="ACHBFW_002503"
     CDS             4117..4836
                     /locus_tag="ACHBFW_002503"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM9318768.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            5315..>5529
                     /locus_tag="ACHBFW_002504"
     CDS             5315..>5529
                     /locus_tag="ACHBFW_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM9318769.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENF"
BASE COUNT         1666 a         1007 c         1122 g         1734 t
ORIGIN      
        1 aaatcacagt cccttttttt cttagaaatg ggaaaccact agtatataaa taccattatt
       61 cttcaatcaa ccgcttcacg attgcataat ccccttcata aggttcatct acaccattga
      121 ttttttgata gggatccacg ccttttcccg caataacgac tgaatcttgc ggagcgcttt
      181 ctgctaaagc gcgtcgaatc gcttcaggac gatccatttc ataatgaacg atcaaatctg
      241 gattagtaat tgcttcattg atttcttccg cgattttttt cggatcttca aatgctgggt
      301 catctgccgt caaaaaggcg atgtcagctg tttctgatag cacttttcca aaatcatgtc
      361 gacgggagat tgctttgttt cctgggctgc ctaatacaac aattacacgg ccatctggat
      421 gttcattttt ggcaaattcc agcaatgttt tcaagcttag ataattatgt gcatagtcca
      481 catagacatg cgctccattg gattggatca attgatccat tcggcctggt acacgagctt
      541 cttttaatcc gtcttgtgca tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg
      601 cactggctgc attttcttgg ttgaatcctc cagccagcaa aatatcataa gcagtatcgc
      661 agatagctaa gcgatcttca tgagaagata aagtaaaacc gtgagtacct ttatctgaac
      721 ggatcacttg ataattgctt tcttccgaac gaccataagt gatcgttgga atctggaata
      781 gatcggctgt ttccctaagt aaatcaaaat agtcactttc atgatttaaa acaactgttt
      841 ttgaattcaa gatcaattgt cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga
      901 tcggactgat atggtcaggc gagatattca agaagattcc gacatctaaa gtcaagccgt
      961 atacgcgttg tgttttgtaa gcttgtgaag aaacttccat cactagatga gtcataccat
     1021 tttcaactgc ttctgacatc atacgatata aatctaatga ctcaggcgtt gtcaaatggg
     1081 atttgaaata tgttttgcca tctaacgttg tattcatagt agaaagcaat gctgtttttt
     1141 tatttgttgt gtgatccaag attgcttttg taaagtacgc agcagttgtt ttaccttttg
     1201 ttccagtgaa tccgatcact ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa
     1261 gaagcgccat tgctttacgg atatccgtta cgatgattcc tactgctgaa ttttcgtatg
     1321 gttgttctga tacataatat tgaataccct gttcgattgc tgaatccaaa tattcttctt
     1381 tgaaattcat tcctttacag aaaaacaaag tagaagcatc agcttttctt gaatcataac
     1441 ttaactgaag aaattcagct ttttctggta catcaagatg ccagcctctt gttgaaacaa
     1501 attcttttaa tagattttct tttaatagta aatgacggat gtcttctaaa gagaagctca
     1561 ttgattgatc cctcgcttta ttcaatttct tggtttatta tagcacactc tttttaatat
     1621 atcgtttgtt tccgctatga caaaatccag ctttattcaa attttcttga cacaggggac
     1681 aagcggatga gtattttcat aaacaatgaa ctgactgtaa tagataggag aacgaatagt
     1741 gtctccaaca atccaaaaac ataataattc aaaaagacga taagactatt ggtaagtaaa
     1801 agtccttgcg gcattggaag ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata
     1861 ccgccatttg aggccccgac tgtgtacaat gtgtaaaccc caagactgaa caaaagactt
     1921 cctgaaatca gtgaaaagaa atgtgaggag atcatttcca cttttggaac aatctctaaa
     1981 acaacaggaa aaagcagact tcccgctaca gtcttaaaaa atactgattt tcctaatagg
     2041 acagcggcta gaagcagcat cataagattg atgctccaaa cagtaactga gcgttggata
     2101 gaaaaggccg tttcaactaa atgtccgatg gcgcccactc cggcggaagc tatatcgtga
     2161 gggagcaaga accaattcaa tccaactgtg ataaagaaaa gacctaaaaa tataacgggt
     2221 agttttttta tttcttgtgt cattgatatc tcctataaat gttagccata aaaggctgta
     2281 tatttcttta ctttaccaaa aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa
     2341 agttatataa aatcgattgc cagtttctgt tttaccaatt aaaatgatag taatgttttg
     2401 tagaaagttg gaatctgacc gatggataat caaagaaaac cagcattaga tcaattaaca
     2461 ccgcaagaaa aaatggcacg aggctctgct tggatgaccg ccagtaatat catttcaaga
     2521 ttgctaggcg ctgtctatat cattccccgg tatgcttgga tgggcgaaaa tgctaaggct
     2581 gccaatggat tattcaatat gggatacaat atatatgcat tgtttctact gatctctaca
     2641 gcaggaatac ctgctgcgat tgcaaaacaa actgcacgct ataattcatt gaatgaatat
     2701 ggcacttcac gacgattatt tatccgtgca ttacagatga tgggcgggct aggcctgttg
     2761 tttgctttgt tcatgtacat cgcctctcct tggctagctc atgcttctgg tggcggagaa
     2821 gaactgattc caacgatgcg ttctttgagt atcgctgtat tggtttttcc ttgtatgagt
     2881 gtgatacgag ggtattttca aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt
     2941 gtagaacagg tggcacgtat tttttatatg ctattggcta cattcatcat catgaaagtc
     3001 atggatggtg actatgtgac ggcggtcacg caatcaactt tcgcggcatt tatcggtatg
     3061 ctagcaagta ttcttgtcct tttgtatttt ttgaagaaac atcaagcgta tacatcggcg
     3121 tttattcact acagcgaaaa caaaataaat attgcaacga aggaattgtt gttagatacc
     3181 gttaaagaag cgattccgtt tattattgtg ggttcagggg taacgatctt caaattagtc
     3241 gatcagttca cttttatgaa gatcatgtct gcatccaccg agtattccaa tgcacagtta
     3301 ctggatcttt tttcgatttt tagtgccaat ccagataagt tgacgatggt agttattgca
     3361 ttggcaacat cgatcgcgtc tactgggtta ccgttgatta cagaagctgt tacgttgaaa
     3421 gatcgagtag gtctggcaaa attgacaagc ggaaatttac agttgttttc attttttatg
     3481 tttccggcga catttggtgt tatgttactt gcctatccgc taaatacatt attttataca
     3541 ccggattctc taggaagtaa tgtactgatc caagcaagtt ttgtaggact cttcctagga
     3601 ctatatatgc tagtttccaa catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat
     3661 tatttggtta ttgggtttct agtgaaactc cttcttcaat acccagccat tcgtttattt
     3721 gaagtatacg gccctttatt ggcgacaatg atcggatttg ctgtgtcttg taccttgatc
     3781 ttaaagagaa tccatgaagt agcccgcttt aaaccaagtt ttgtctggcg caggacattg
     3841 ctgatcttta ttcttacctt gattatgctt ttggcttcgt ggatcacaaa gatgattttt
     3901 ggtgcttttt taaacgaaga tagcaaattc cagtctctat tgttgattct tgtagtagca
     3961 ggtatagggg ctttagtgta tggttattta gctttgaaaa tccgcttggc agaaagacta
     4021 ctaggaccag gaatagcgcg tttacgacat aaattaaaaa taaaataagt gaatcagaag
     4081 agctagattg atgctctttt gaaatggggg aaacgcatgc gtttagataa gtttttagca
     4141 gatatgggac ttggcagccg caaagaagtc aaaggattga tcaaaaaagg aacgatcgaa
     4201 gtcaatggac aagtggtgaa aagcgataaa gcgcaagtaa atgaattcga agatgcggtt
     4261 acttatttag gagaaatcat tacgtatcaa aaagattttt actacatttt gcataaacct
     4321 gcaggagtca tttctgctac tcaggataat catgatcaaa ccgtaatcga tttattggaa
     4381 gaccaggatt atcgggaaga tctttttcca gtcggccgat tagataaaga cacagagggc
     4441 ctcttgattt taacgaatga cggggtgttt gcccatcagt tactatcacc taaaaaacat
     4501 gtggagaaag aatatctagc tgaggttagc ggtataatga cagaagaaga tatccgttta
     4561 tttgcagaag gactgattat tgatggcgat gagcagacat tgccggcaaa actccaaatt
     4621 gatcaagtag atcaagagac agaaacttca attatccgtc tgattttgca tgaaggaaag
     4681 tttcaccaag ttaagcgaat ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga
     4741 atccgtatgg gaaaattttg gctgcctaaa gatttgaata aagggcaata tcgacccatg
     4801 accaaagaag agctagaaca agtgagagaa aaataattga atggatagct aaaagaataa
     4861 gcagagtagt tgtagcgcca tctgaaaagc agacttgtcc gtctgacttt cggatggctt
     4921 ttttgtgaaa tttcggctta tttccgtaga agctacttct ggaacaccgt ttgttcggaa
     4981 taagggactg tgacaagact tttgttacag tccccattgt tccttgtgac tcagagttag
     5041 atagtaagga cacgatctcc ctaatactca ggccaaaacg ctgaattcac aacctctgat
     5101 aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa
     5161 ctattttccc gtttttttct ctaatactca ggccaaaacg ctgatttcac aacctctcct
     5221 atactttgtt tcattttccc tatactttgt ttatttattc tcattattga aagcgctata
     5281 attacgttgc gaatgaatcg aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca
     5341 tttttttatg aatctatggc gttataaagc acttgtcctg atggctgtac cgggtatgct
     5401 ttggatgatt tttttctttt acatacctgt aatggcgaat gtcgtagcat tcaaaaattt
     5461 tcatatttca gcagatgggt tcttggctag cttgagggaa agtccttggg tgggttggga
     5521 aaatttcaa
//
LOCUS       DAYYME010000149         5522 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000149 DAYYME010000000
VERSION     DAYYME010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.150"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..445
                     /locus_tag="ACHBFW_002505"
     CDS             128..445
                     /locus_tag="ACHBFW_002505"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9318770.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            475..795
                     /locus_tag="ACHBFW_002506"
     CDS             475..795
                     /locus_tag="ACHBFW_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM9318771.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            903..2363
                     /locus_tag="ACHBFW_002507"
     CDS             903..2363
                     /locus_tag="ACHBFW_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9318772.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            2431..2652
                     /locus_tag="ACHBFW_002508"
     CDS             2431..2652
                     /locus_tag="ACHBFW_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318773.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            2683..2865
                     /locus_tag="ACHBFW_002509"
     CDS             2683..2865
                     /locus_tag="ACHBFW_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFM9318774.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            2865..3278
                     /locus_tag="ACHBFW_002510"
     CDS             2865..3278
                     /locus_tag="ACHBFW_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFM9318775.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            3401..4582
                     /locus_tag="ACHBFW_002511"
     CDS             3401..4582
                     /locus_tag="ACHBFW_002511"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9318776.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(4653..4817)
                     /locus_tag="ACHBFW_002512"
     CDS             complement(4653..4817)
                     /locus_tag="ACHBFW_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318777.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            4971..5044
                     /locus_tag="ACHBFW_002513"
     tRNA            4971..5044
                     /locus_tag="ACHBFW_002513"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5005..5007,aa:Arg,seq:cct)
     gene            complement(5113..>5522)
                     /locus_tag="ACHBFW_002514"
     CDS             complement(5113..>5522)
                     /locus_tag="ACHBFW_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9318778.1"
                     /translation="SNRKISIDKHTIDLLKKFKINQKELFENFEIKNPHNFVFFNLKN
                     GLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNILSVSKRLGHSSLE
                     TTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT         1769 a          913 c         1144 g         1696 t
ORIGIN      
        1 actaaacatc tgtgtgcgca ctataaaaaa gagctaaaaa gcatgaaaac gctatttcag
       61 ctcttttttt tattggcatg cttcttgcgt tatataaaag tgtaaaaaaa taaaaggagt
      121 gttaaaaatg gcaaaaaaaa caatcatgtt agtttgttca gcagggatga gtacgagttt
      181 attggtcaca aaaatgcaaa aagcagcaga agaaaaaggg atggaagccg atatttttgc
      241 ggtctctgcc tcagatgcag ataataattt agaatctaag aatgtcgatg tattattatt
      301 aggtcctcaa gtacgtttca tgaaagctca atttgaacaa aaacttgcgc ctaaaggtat
      361 tccattagat gtcatcaata tgcaagacta tggcatgatg aacggagaaa aagttcttgc
      421 acaagcagaa aatctaatga aataaatcaa gtatgcagtt gggggtataa caacgtggaa
      481 gatcaacaaa atctagaagc catcatgggg ttaatcatgt ttggcggtaa tgcaaagagc
      541 gatgcaatgg aagcgattgc ggctgctaaa atcggtgatt ttgagttagc ggataggaaa
      601 atcgctgatg cagaagaatc acttatacaa gcgcatcatg cacaaacaga catgttgaca
      661 caagaagcac aaggaaatca tatgcaagtg acattattga ctgttcacag tcaagatcac
      721 ttaatgacat ctattgcgtt tactgattta gcaaaagaaa tcatcgaact ttatcgtcgc
      781 gtatacagcg aataaataca acttcttttt tgaacggaag aagaaaaatt tttgaacgga
      841 ttttttattt tgttataagg agaactctct ttatataaaa aacttatagg aggaagtaaa
      901 aaatgaacgg actatcaagt tggatggaga aatacatcct gccggttgcc gcaaaaatcg
      961 gagcgcaaaa acatttagtt gctttgcgtg atgcttttat cggcatactg cccgcaacaa
     1021 tggcgggatc agtcgctgta atgatcaatg caattattcg tgatttaccg caacagttta
     1081 acagtaccta tgctgacagt ttagcagcag acaaaggaat ctttgccgta ttgaatgtca
     1141 tcatcggaat caatggtttt gtctggaacg gtacactagc aatcgccgga ttgatctttg
     1201 ccttttcttg ggggtataat cttgcccgag cttatggagt aaatgatcta gctgggggga
     1261 tcgttggttt agctaccttg attcaaggta tttcctttgg tccagcatta tcagcagcat
     1321 tagatgtgaa tgttccttca aatgtcgcag atgcaatcaa tagttctgag attggtgcaa
     1381 cgattgcaga tggcacactg tcagttggat tatggggttg gctaaaacta gatcatttga
     1441 atggaaacgc ttattttaca gttatgatca tgggagctat ttcagtcatt attttttgta
     1501 aattgatgct tgccaacatt acgatcaaaa tgcctgattc agtaccacca gcagtatcta
     1561 aagcatttgc agccatttta ccagcgacga tcgctttata cgtcattgct attatcaatt
     1621 atatcgtagg tcgtgtagct gatgggcaac tgatcattga tttggtgcaa aaatatattg
     1681 cagaaccatt cctaggatta tctcaagggt tgggagcagt attgatcgtc acagtatttg
     1741 ttcaagtatt ctggttcttt ggtattcatg gatcaaatgt attggcacca gtgctagagg
     1801 gaatctgggg acaagcacaa ctaatcaata ttgatatttt ccaaaaaggt tataacggaa
     1861 aaactggtac accagccgtg ttagatgcaa tcgaagatgg aaaagcttat atgtgggtcc
     1921 gcggatcgtt cgatgctttt gcatggtttg gcggatcagg cggaacgatc gtcttactga
     1981 tagctatctt gattttctct aaacgtgcag attatctaac agtagctaaa ttatccattg
     2041 ggcctggtat cttcaatatc aacgaaccag tgatgtttgg tctgccaatc gtattgaatg
     2101 caatcatgtt tatcccattc ttgattgctc cagtagtagc aacagcaatt ggatggacag
     2161 ctacttatct tggcctagtc gctccggtat cgcagcaagt cacatgggta gtaccgccgt
     2221 tcttattgtc attcttagca acaggagcgg attggcgggc accgatcgta acattagtat
     2281 gtatggtcgt gactttcttg atttgggcac catttgtcat ctctgcaaac aaaatggacc
     2341 ctgccgcagg cgaacaacaa taagatgaac gtgaggagcg ggacgcttta agtttcgttc
     2401 ctcataatga attttcgagg aggaactgaa atgattacaa ttgccagtgc aatgcaacaa
     2461 gatgaaatca aagaattgtt agaaggatac aatgaagaag gaatgacttt tacatttaaa
     2521 gaaaaacaag gaatcaagct gttctttgaa acaagtgcag aagatccgga agttgcggct
     2581 aaaaaagcaa aggaactaat caaagcacag ccttggggaa cagtccttta ttttcaatca
     2641 acagctgttt agaaaatggt ttaaaaagat aggatggttg agatgataaa aaacggttta
     2701 tttttatgta gtattggttc tttgatcgtg ttgtatagtt tgaatccaca ttcgcaaagg
     2761 tatgatttgt atagtatggt cacaggtctt tttctaacag gattaggaat ctatttcttc
     2821 atgaagggga aaaaaagaga agaagcaaag gagaaaaata actaatggaa atcacgaaaa
     2881 aattaaatat tccagcatcg tttttttacg ataaagttat ggactctgtc cttttcgatg
     2941 tacgtaaagc gaccggcaaa tcagtaacta gaaaacagct gaaaaatttg gagtacgtca
     3001 aacaattctc gcaaaataac cgggctcgca taaaaatcga agaagtcatt gaaaaccagt
     3061 cttataagtt tcgtacatcg acaacgaaga atgaattcgt cgttcattat caaatcaaac
     3121 cattggacga ttcgacctgt gaggttcatt acacagaaaa aatggagtct tatggcatgc
     3181 ttcaaaagtt gaatgatatg cttttaggaa caatcttgat gtattttaaa cggagacgtt
     3241 tcaaaaaaat gctagatatg atcgaagcaa cgtattaaat caatgaaaca tggtactatt
     3301 tagttattga aaaatgaagt aagctagaag gaatttttca taggaagata gatctttcct
     3361 gcgaatcctc actagctttt ttgtaggaag gggcagcaaa atgacaaaca aagtaagaga
     3421 gctaggtgta tcttcgattg aggaaatgtt tgatttagcc acttatgcat ttaatggggc
     3481 agatacacct gaaaggcgag aacgttttcg tacacttgcg gagaattctt ggaattacgg
     3541 attttttgat gaggaggatc ggctaacaag tcaagtcatg gcgacccctt ttccagtgaa
     3601 tttttacgga caagagtacc taatggccgg cattggctat gttgcttctt atccagaagc
     3661 aaggggacaa ggcgggatca atcagatcat ggaaaagatc cttgaagatt gccgtgatcg
     3721 taaagtaagc ttatcttatc tggcgccatt ctcctatccc ttctatcggc gctatggcta
     3781 tgaacaatca tttgataaaa tcagttatca actaaacagt cgtgatgtgc cgtttatcaa
     3841 aaaaaaatct ggaaaaataa agcgtatgga ttttgaagat gcaaaagctg caatcagaag
     3901 aatttatgaa cagatgccgg aaaaccaacg agctggtctg aagcgcgaag actggtggta
     3961 tacgtataaa ttcaagcaaa aaggcaatta ccagtttgct ttgcattttg atgataatga
     4021 ggaagcaacg ggatatatcg tttatcagct tgcaacgtct tcctttatta ttgcggagtg
     4081 gggatttttg gatcatgatg ctttttgcag ccttgttcgc ttcgtaagtt ctcacaatgg
     4141 tgcttttgaa acatttgaat attcatgcgg ctacggcggt aatgatcaaa attatctatt
     4201 agaaaatcca tttgcttctg taatgatcac accttatatg atggcgagga tcgtagatat
     4261 ttctttgttt ttggaaaatt atccatttaa aaagagacaa gctgcatttg ccctacaaat
     4321 agaagaagat cattatgcga aatggaatga aggaatcgtg gaagtaacaa tcgaaaatgg
     4381 aaaaaatcat ctctcaaaag tagaacaaac aaaactccca atcgttcgag gaagtatcca
     4441 acatatcacg cagctattat tagggtatcg taaagtggaa gagttggtat tccaagaaag
     4501 aatacaggtg acaaaagaat atgaagaaac gttaagctca atcttgccac agcaacagcc
     4561 aattcttaat gactattttt gataaaaaac gggtgatgac aatatttttg tcaccaccct
     4621 tttgtttttc atttggcatt tgaaggaaaa tgttaaagta aatcctcttt tttcgtaaat
     4681 ttgaattttt tgttttcttt tgttttaggg ttttcttttt tgtggtcttg gattgcttct
     4741 ttcgtaatct tttcccgatt ttttttagat gaatctaagc gttctgcgta tactttttct
     4801 tttggcttat cattcattgg aaaggacctc cctacattat ttcattctac ctctagttta
     4861 tcatctgtta aggactagtg gaaacgtttt gcctaactta taagctgatt tttttagcag
     4921 ataagcttgc aagtaaaaaa caaacaagct acaattgatt tattgaaatt gttcctgtag
     4981 ctcagtagga tagagcaatc gcctcctaag cgataggtcg ctggttcaat tccagtcagg
     5041 aacgtataaa taacccctgt atcctttgcg gtacaggggt ttgttttgta tttgtgccaa
     5101 aaatgtgcca aactagttat catttttata taaactatcg aacacagtat tgattttttc
     5161 tttatcctga tcttcaagct ctcgaacaat atgaagataa gtagacattg tagtttctaa
     5221 actgctatgt cctaaacgtt tcgatacgct aagtatattt actccttgat aaagtaaaat
     5281 agatgcatgt gtatgcctta gtccatgcaa agtaaattgc ttttcaatcc ctaattcttt
     5341 taattttttg cgcaaatatt tgctgacggc gtttgatgaa actaatccat tttttaagtt
     5401 gaaaaaaaca aaattatgag ggtttttgat ttcaaaattc tcgaataatt ctttttgatt
     5461 gattttaaac ttttttaaga gatcaatcgt atgtttatca atggaaattt tcctatttga
     5521 ag
//
LOCUS       DAYYME010000150         5344 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000150 DAYYME010000000
VERSION     DAYYME010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.151"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1077
                     /locus_tag="ACHBFW_002515"
     CDS             <1..1077
                     /locus_tag="ACHBFW_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318779.1"
                     /translation="FKENDYLKNKEIIIPEFDSRNILLALVRYVNWEGYENKNPYVEE
                     ACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEEDKKSAETKPLFQ
                     LVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVYKNFVLNDLSL
                     NELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLSEKEQLLYGKI
                     VEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVSESSVKQFFYR
                     TQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGLETEKTKIYPI
                     SLKGANIHLYHIAQELHLLKKHIS"
     gene            1298..3061
                     /locus_tag="ACHBFW_002516"
     CDS             1298..3061
                     /locus_tag="ACHBFW_002516"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFM9318780.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3145..3603
                     /locus_tag="ACHBFW_002517"
     CDS             3145..3603
                     /locus_tag="ACHBFW_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFM9318781.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3712..4395
                     /locus_tag="ACHBFW_002518"
     CDS             3712..4395
                     /locus_tag="ACHBFW_002518"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM9318782.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4542..5156
                     /locus_tag="ACHBFW_002519"
     CDS             4542..5156
                     /locus_tag="ACHBFW_002519"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFM9318783.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
BASE COUNT         1902 a          842 c         1101 g         1499 t
ORIGIN      
        1 tttaaagaaa atgattattt gaaaaataaa gaaatcatta tcccagaatt tgactcgcgg
       61 aacatcttgc tagctttagt acgttatgtc aattgggaag gctatgaaaa taaaaatccg
      121 tatgtagaag aagcttgtaa cgagttgatt gattatgtag agactcattt ttttaaaaga
      181 agatattcgg aagatgaaaa gtcctttatc atacgaggaa tagagatcgc aatcggaaga
      241 aaggcatttc cccttggact gaaagaagaa gataagaaaa gcgcagaaac gaagccgtta
      301 tttcaattag tctgtcaagg attggaagag caaaaaggaa ttctagactt acaagaagaa
      361 gatgtctatt atattttttc tttgttcaac agtagaaatt ataccaatga gaacatggag
      421 ctgttaagaa aagatttcga agttgtttat aaaaattttg ttctcaatga cctatcactg
      481 aatgaattga ttttacagat tattagtatc gctgaaatcg acgatgcaga aaattttatt
      541 ttcaagcaat cctttttaca atttgtccgt acgttgtggg cagatggaca agtattttta
      601 ccggaaagga tctatcttct atcagaaaaa gaacaacttc tgtacggaaa aattgtagaa
      661 atattggaag agtggaaaaa gaaaaatcaa atccaactaa gatggaatga aaatttcatt
      721 cgaaaattcg tcaaaagtct ttctttgatc aacagtgaac aagcggggag acaggaaata
      781 gaaatttttg ttgtttcaga aagttcagtc aaacaatttt tttatcgaac ccaatttcat
      841 ttgtatgcgg aagaaggcat cgctgaaata aatcctattt tatttcgttc attagaagaa
      901 ttgcctgatg aatgtctatg tgcgaaaaaa agagtggttt tgtgcgatgc tgctagctat
      961 caaaatggat tagaaactga aaagacaaag atctatccaa tctcattaaa aggtgcaaac
     1021 atccatttgt atcatattgc tcaggaactt cacctattaa aaaaacatat ttcttgatga
     1081 gaccttaatt aggagacctt ctacaaagct ttttaattgt ctgtagaagg tttttatttg
     1141 agtcctgatt cgtgcgaaga tctacttata taacaagctt tctctgaaat ttttttaaaa
     1201 tgctattgac ttagagtcaa ctctaagtag tagaatagat aaagtgtaac aaatctattt
     1261 tcattaaaag aagatagtac ggaggagaga atgtattatg agtacagtat tacatgtatt
     1321 ggatgcaatc aatagccccg cagatgtcaa aaaattatct gttgaggaaa tgaaacagtt
     1381 ggcagaagag atgcgccaaa aaatcttgca aaaggacagc gatgtaggtg ggcacgttgg
     1441 tccgaatcta ggtgtagtag aattaacgat tgcgtttcat tatgtatttg attcaccaaa
     1501 ggataaaatc gtttgggatg tttcccatca gtcttatggt cataagcttt taactggtcg
     1561 aaaaaatggt tttgaaaaag gacattaccg tgaagtgagc ggatattcca accaacatga
     1621 aagtaaacat gactatttta cagtggggca cacatctaca tctatcagct tagcaacagg
     1681 tttagctaaa gcgagagatc tgaagaaaga atcaggaaat gtggtagcat ttatcggtga
     1741 cggctcccta agtggtggct tagcttttga aggattgaac aatggtggtg ttttacaatc
     1801 caatctaatc gtattagtaa acgataatga gatgtctatt gatgagaatc atggcagttt
     1861 atataaaaac ttagctgagc ttcgtcaaac caacggtaaa agcagcaata acctatttaa
     1921 agcaatggga tttgattatc gttacgtgga taatggaaac gatctggaaa cggtgatccg
     1981 tttatttgaa gaagtaaaag atattgatca tccgattgta cttcacgtcc atacggaaaa
     2041 aggtcgcggt tatcagccgg caatcgataa taaaatgaaa tatcattggc atgtaccatt
     2101 tgatctgtcc accggggagg caaagcaagt aacttctgga gaaacattta atatgatcat
     2161 catggattat ttggataaga aaatgcaaac aagtacagct cctattgccg cagtcaatgc
     2221 agcgatacca ggcgcctttg gactgaaaac attccaagaa aaacatccag atcattattt
     2281 tgacgtgggg atcgctgaac agcactctat cactttcgct tctgctttgg cttctcaggg
     2341 agtacgtcca gtcgtcttcc aatcaagtac tttcttgcaa cgggcatatg atcaattatc
     2401 acatgattta gcgatcaacg agaatccagt gattatttta gtcggtggcg gaacgatctc
     2461 agctagtgac cgaacacacc aaggaacatt tgacattcct tacttatcaa gtatcccgaa
     2521 catcaaatat cttgctccag caacaaaaga ggatatgatc tctatgctag attgggcgtt
     2581 cgaacaaaca gcgcagcctg tagcaatccg aattcctaca catggtgtag aacatggtac
     2641 agctatcgca aatgattata gtattccagc ctatcaaaca gtaaataatg gaggaaaagt
     2701 agcaatttta gctttaggcg gttttcttga attaggaaaa gaaacagtaa aagcttttag
     2761 aaaaaaagga atcgaagcaa cgttgatcaa tccaggaacg atcacagaaa atgacgtaga
     2821 aacattagat aaattaaaag aaacacacga attagtcatc acattagaag atggtagttt
     2881 gtcaggagga tttggcgaaa atatcagtcg ttattatggt gattcttcaa tgaacgtatt
     2941 aaattttggt gcagagaaag aattcacgga tagcgttcct gtagggcagc tttatgaacg
     3001 ttatcattta acaccaaaac aaatcgttga agatagtctg cgtgtcttga atattcaata
     3061 attcattatg ctcaccatcc agataattgt ttatactagt atctggatgg ttttttcatt
     3121 ataaaaagca aaggggtgga tgttatgtat cagcaagtag aagaatatgt caaaaagttc
     3181 caagaaaaat atcctagtta tcaatcagaa aaattaggtt tcttttcatt tggggataca
     3241 gaaaaaatgg cagacgagtt gagcgaacta gttgtaaaaa agataaagac tggtacatca
     3301 agtggttacg atctgtataa acaagagaat gagcctttac caaaagttgg tcaattagac
     3361 gtggtcctga atggaaaaaa tgaaccagtt tgtatcatca aaaatataaa tgtgaccatc
     3421 ttgcctttta gtgaagtcac gaaagagcat gcttggaaag aaggagaagg ggatcggaca
     3481 ttagcttatt ggcggaaagt ccatgctgag ttttttaaac atgcttataa agaagcgttg
     3541 aacatagaat ttacagaaga gaagctggtc gtatacgaag aatttgaagt aattttcttt
     3601 tagtaagaaa ggtctgctga aagaaatagc tcattattgt ctcctgaata gaaaagggtt
     3661 acatttttat atgaagctgt atttttatat aacattactg gaggagatat tgtggataca
     3721 ctggaacaaa tcaaaggcgg agtcgtcgtt tcttgtcaag ctttggaaaa cgagccgcta
     3781 cacagttctt ttattatggg gagaatggct ttggctgcaa aagaaggcgg agcagttggt
     3841 attcgagcaa atacgacaga agatatcaat gaaatcaagc gaatcgttga tttaccggta
     3901 atagggatta tcaaaagaga ttacgatggg tcagaagtgt ttattactgc gacagaaaaa
     3961 gaagtggaag aacttttgga gacgcaagca gaaatcattg cactggatgc aacttctcgt
     4021 aaacgtccga cagaaaaaac aactgctgaa cttgtccaaa tgattcacga acacggtcgg
     4081 ttagctatgg cagatatttc gacttttgag gaagcgcttc aagcgcaaga agatggtttt
     4141 gatcttttgt ctacgacttt agcaggctat acagaatatt cacgaaagac gcagacacct
     4201 gattttgatt tattgaatca aatcatagaa caagtcgata tcccagtaat tatggaagga
     4261 catactgact ctcctgaaca agtagagaaa gcatatgaat taggtgcttt ttcagtcgta
     4321 gttggatcta ttatcacccg tccgcaattg atcactaaaa gatatgtcga agccgcaagt
     4381 aaagcaaata actaaaaaac acgcacggca cagattcttg acaaaaaaga aaaattgata
     4441 gatagtttaa agcagattct aagaagatga aaaaagcatt acttttcatt ttgtcttcaa
     4501 gatctgcttt ttaacgaatt ttatagaaag aaggcgaaac catgtttgaa cggagtcctt
     4561 cagaattgac agaacgtgaa aattacaagt tgctgattgg ttcagtcatc cctagaccag
     4621 tagcagtagt aacgactaaa tcagcaaaag atatagtgaa tatcgcaccc ttcagttact
     4681 tcaatatcgt cagttcgaat cgacccatct tatccttggc gatccagaga aaacaagatg
     4741 cagtaaaaga cacagcaaag aatattcttg agacaagaga agctgttatc catatccttg
     4801 atgaggacaa cgtagaagaa gtaaataaaa cagcagccaa tctgcctaat gaggaaagcg
     4861 aactgtacct gactgacttg actttaactg attcagtaat catttcagta ccagcattga
     4921 cagaagcaaa agttcgtttt gaaacatcct tatatgaaca tgtcgagata cggaataaaa
     4981 ataaagtaac agctgattta tttttgctag aagtacagaa atatcattta aatgaaacag
     5041 tctatgatga taagacagga aggatcgatc caagaaaatt gaatgctgta agccgtttgg
     5101 caggaaatga ttatgcagca ataggagaaa ttttcacgat tgcacgtccc gaataaaaag
     5161 gagcactagt tatgttaaaa ataagctata aaccatcgac agattcaaaa gaaatgaaaa
     5221 aagaatatga aacagtgaat gattttctgc aagggcagta cttagaagtt ccgccattgc
     5281 aagaccattt tgtcgttact actgttactt tagatggtaa ggaaatagaa atgccagatc
     5341 aaac
//
LOCUS       DAYYME010000151         5331 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000151 DAYYME010000000
VERSION     DAYYME010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.152"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            31..822
                     /locus_tag="ACHBFW_002520"
     CDS             31..822
                     /locus_tag="ACHBFW_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9318784.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            788..1741
                     /locus_tag="ACHBFW_002521"
     CDS             788..1741
                     /locus_tag="ACHBFW_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HFM9318785.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2139..3113)
                     /locus_tag="ACHBFW_002522"
     CDS             complement(2139..3113)
                     /locus_tag="ACHBFW_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9318786.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3228..3662
                     /locus_tag="ACHBFW_002523"
     CDS             3228..3662
                     /locus_tag="ACHBFW_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9318787.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(3787..4809)
                     /locus_tag="ACHBFW_002524"
     CDS             complement(3787..4809)
                     /locus_tag="ACHBFW_002524"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFM9318788.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            5201..>5331
                     /locus_tag="ACHBFW_002525"
     CDS             5201..>5331
                     /locus_tag="ACHBFW_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HFM9318789.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGL"
BASE COUNT         1681 a         1016 c          951 g         1683 t
ORIGIN      
        1 attgactttt atgttaaagg agtaattcat atgctgaaaa aagaaagaca agaacaaatc
       61 attacattat tagagaaaga tttttattta actaattcag aaatcgctaa caaattaggc
      121 gtatcagaaa tgaccatacg ccgagatgtc actgaattag caaaaaaaaa taaagtaaaa
      181 aagctatatg gtggcgttga acgaaaggaa ccttataatc gagaactatc gactcaagaa
      241 aagattgata cgaatgtagc cgaaaaaaaa tatattggtc aagtcatgaa cagtatgatt
      301 cccgataatt ctactattta tgtcggtgct ggaacaacga tcctatatgc acttcctttc
      361 attcaaaaga aaaatctatt tgtaattaca aacagtttga ttgctttcaa ctatttaaag
      421 aatcatacaa gttatcggat tttacttact ggtggcgaat tttctcctat tacagaagag
      481 ttcattgggg aagttgcaga aagatctttt gaaagtttga atgtcgatat ctctttcgct
      541 gcaacaaacg gcatatttga taacaatgtg acaaccgctc aatttattga gggaaatatt
      601 cagaacgcag cgctcaatgc agcaaaaatc aaatgcattg ttgcagatta ttcaaaatta
      661 aataaatcag acgtctatac ttttagaaaa ttaaccgatt ttgattatct aataaccgat
      721 aatcatttat cagaacagga tttcaatcat tattcaaaat acttaacaat attaaaggag
      781 cccaaaaatg atactgacaa tcacgatgaa cccatccatt gattccattt attttacaaa
      841 acactttgaa ttaggtacga tgacacgctt tgattctccg gtacgctatg taggcggcaa
      901 gggaatcaat tgcggacgag cttctgctct actaggttca tcagttattt taacagggtt
      961 tttgggcggt tattttggca gtatgattca agatatgctg aaaaatgaaa atttgtttga
     1021 tttgcatttt ttacctattg cagaagaatc aagaggtgcc gtcacgatca tgcatgatga
     1081 tgaaacgcag acagagctag tggaagctgg tcctgaaata gcagatacta ctatcaaaaa
     1141 attaatgaat gatatcaaac taatcagtaa aaataattac ctttcagtca ttacaataaa
     1201 tggttctgtt aacaatccag atcccttttt gtatgctaaa ttattacaat atatcaaaga
     1261 acaaatagat ccttccacct atatattaat ggatatatct ggacaacatc tccgtgaagt
     1321 cttagcaagt aagtataaac cgatgttcat caaacctaat atccatgagt tttcagatat
     1381 catccatcaa aaaattactc aaaaaagcga cataaaacac catctaaaaa caaatccact
     1441 atttaaagat atcgatgtta tcatgatttc ttgcggctcg tatggtgcag tagtcaaagt
     1501 aaaagaagaa ttttggaatg ttgagatccc taagattcct atcattaata caacaggctc
     1561 tggggatgcc actgttggag gttttgctca ttcattagaa cagaattatc ctttaaaaga
     1621 atgtgtaagg tacgcaatgg cttgcggcat gtcgaatgca cagcaatcag aagtcgggaa
     1681 agttattcca gctgatgtaa aaaatttagc aaaaagcatt aaaatcacag aaatgaaata
     1741 aatagtacaa agtaaataat tgtttatatg aaatgagaca caaagagact gtgacaaaaa
     1801 acgtgtgtac cgacccccaa aagttagatt ttttggtcta acttttggga gtcgatacat
     1861 tgtcacagtc cttttttcga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
     1921 caatattcaa caaacatgag aagctatttt ttgaaattgc tccttgtgac tcagagttaa
     1981 acggcaggct cacgacctct tggtataaac ggtgttcaaa agcttgcatc tacgctaatg
     2041 agtccaacca agtcaaaaat gaggagcttt tttggctgtc cttattactt gatgcagggc
     2101 agttttttca taatctcttt tagattaaat ccttttggtt acacaaggtt ctcttcaaag
     2161 aatgttgcca ttttttcaaa tggaatgatg tctgtctggt cgtatagatc tgtatgggat
     2221 gctcctggaa tgatcatgag ttccttgttg tcacctttta gttctttgaa agcgtcctcc
     2281 ccaaagtaac gtgaatgagc tttttccccg tgaatgacta agactgcatg acggatttct
     2341 ggaatataat gcaatagttt tgcattcata agtgacaacc ctgatgtcct attccaacca
     2401 tcattggagt ttaaagaacg gacatgatat ccgcggtctg ttttataata cgcatgatag
     2461 tctttgacaa attgaggagc atcttcaggc aacggatcaa ccacacctcc agcacgtgcg
     2521 tagtcccctt gtttgaaatc aatgatgcgt tgtgcattta attcttcacg catttcatat
     2581 cgggcatttt catccataac atcgaagtag ccatttgctg ttactcgagt catgtcatac
     2641 attgtggaaa caactgttgc tttgacccgc gtatcgatag ctgctgcatt caatgccata
     2701 cctccccatc cacaaatacc aatgattccg attctctcag ggtttacatt tttttgtaca
     2761 gataaaaagt ctactgccgc ttggaaatct tccgtattga tatcaggcga agcaacatat
     2821 cgaggtactc ctccgctttc tcctgtaaac gatggatcaa acgcaatcgt taaaaagcct
     2881 ctttcagcca tcgtctgcgc atatagacca gacgattgtt ccttcactgc accaaatggc
     2941 ccgctcacag cgattgccgg caatttcttt tcacttactt cttttggttc atataaatca
     3001 gcgactaatg tgataccaaa acgattcggg aagctcacct ttcgatgatt gacttgattg
     3061 ctttttggaa atactttgtc ccattcttgt gtgatgttta attctttatt catattctag
     3121 actctccttt gatcaaaatt gacatttgtt tttgaatgta ttattatgat aaaacttaaa
     3181 cttgacttta ggtcaaggta aaaattttat tttaggagga tttttttatg tattccattg
     3241 gacaagtgtc tgaaatgttt ggattgcctg tttctacttt acgttattac gacaaagagg
     3301 gattatttcc tgatatccag cgtgtttctg gtattagaaa attttcagag aatgagttag
     3361 aggctttaag agtcattgac tgtttgaaaa aatctggttt agaaataaaa gacattaagc
     3421 aattcatgga atggagcaaa gaaggcagta agacatttga ggttcgtaaa gaactatttg
     3481 aaaaacaaaa agaagtagta gaaaaagaaa tagcaaaatt agaaagagta ctcgctatgc
     3541 taaactacaa aagctggtac tatgaagaag caataaaagc cggcaatgaa gaagccgtat
     3601 tgacaatgat tcctgatgat ttaccccaac atgtaaaaga agcttatgta cattctcatt
     3661 gaacaaacgg ataactcact ctccttctct atgaacttca tgaaaacatg aataaacacc
     3721 tctaactgat tgttagaagt gtttattttt tcactcaagt atcctgcact tacaaaaata
     3781 tcctttttac cgccatccta attctggagc gatatctttt acgattgttt caagcaagtg
     3841 catgttatat tctaccccca aagtattagg aatcgtgaca agtactgtgt ctgcttcttt
     3901 gattgcttca tcttcagcta gctccttgat taattgatca ggttcaccag caaaagtttt
     3961 tccaaaaatc gttgggtttc ggtgataagc aagatagccg acagcatctt gttgcggact
     4021 accgtcttgt ccaaataagc gtcgatccaa atcagtggtg attggctgaa tcgagcgagt
     4081 aactaatgtt cttggttcaa aatcatgacc tgcttgcttc cacgcttctt tatacgcacg
     4141 taattggttt gcttgttgga tatggaatgg ctcgtgggat tcgtaattga ctaatgtaga
     4201 tgattgcaga ttcattccat gttgtgctgc ccagattgcc gtttgctgcg aaccagctcc
     4261 ccaccagatt ctttgtcgta atccttctga ataaggttct atccgtaatt ttcctgatcc
     4321 ttgtgggaac attggttgag gatttggttc ttcaaatggt tttccttcaa gtaattccaa
     4381 gaattcaagg gtgcgttccc gtgttacctc tttgatttct tcttcactgt aatcataacc
     4441 aaagtatttc cagccatcta gaacttgttc aggtgaacca cgccccactc ctagttgcac
     4501 tcttccttgc gtgatgatat ctgtcaatcc ggcattttct gccatataat aagggttttc
     4561 atatcgcata tcaataagtc ctgtaccgat ttcaatctta cttgtctttg cgccgattgc
     4621 cgctagcaaa ggaaacggcg acccaatctg aggggcaaaa tgatgtacgc ggaaaaacgc
     4681 tccgtccact ccgatttttt ctgcttccac cgctaaatca atcgattgca aataagcatc
     4741 ttgagctgat ttcacaagcg atccttgctc catccaatgt ccaaagctta aaaagccgat
     4801 ttttttcaag gtaatgacct cctttaaacc tacaaaaatg gaaggttttt tatttttaat
     4861 atgaactgat tctactactt tttttcttaa aagacgaata attgttctcg cctcacaatc
     4921 aagaaatgta tcggcttact acacccctac tttcttcatc atctgccgtg tcttaatctc
     4981 ttttaacagt ctgttacatc gttgattttt gactcaaaat gattgtttct aacatgtatt
     5041 tatggtaaaa aagacaagat tagttctttg tacacatcga tttatacgct gaacaaaaag
     5101 aaatatataa actaagaaac ccattgtttt ttcgctccat cacatagagt ttactttaga
     5161 taaacagtga catttactaa tccataacgg aggaaatcaa atgaaaaaaa tctatttctt
     5221 atgtacgggc aattcatgcc gcagtcaaat agcagaaggc tacgcgcgta aatacttgtc
     5281 tccttcaatg tttgagataa gaagtgcggg gatcgagaca catggcttga a
//
LOCUS       DAYYME010000152         5259 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000152 DAYYME010000000
VERSION     DAYYME010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.153"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..378
                     /locus_tag="ACHBFW_002526"
     CDS             22..378
                     /locus_tag="ACHBFW_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HFM9318790.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            394..744
                     /locus_tag="ACHBFW_002527"
     CDS             394..744
                     /locus_tag="ACHBFW_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HFM9318791.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            773..1552
                     /locus_tag="ACHBFW_002528"
     CDS             773..1552
                     /locus_tag="ACHBFW_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HFM9318792.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1563..2225
                     /locus_tag="ACHBFW_002529"
     CDS             1563..2225
                     /locus_tag="ACHBFW_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HFM9318793.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAIFPA
                     VN"
     gene            2244..3350
                     /locus_tag="ACHBFW_002530"
     CDS             2244..3350
                     /locus_tag="ACHBFW_002530"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HFM9318794.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3340..4431
                     /locus_tag="ACHBFW_002531"
     CDS             3340..4431
                     /locus_tag="ACHBFW_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HFM9318795.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4444..5184
                     /locus_tag="ACHBFW_002532"
     CDS             4444..5184
                     /locus_tag="ACHBFW_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HFM9318796.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
BASE COUNT         1685 a          864 c         1254 g         1456 t
ORIGIN      
        1 ttttacgagg aggaaacaat catgattaaa gtagttattg cagaccgtat gggtaaagga
       61 caaaatgtgg ccaaaggggt agaagcagct ggtggaaaag cagttgtcgt accaggaatg
      121 ggggcagata tgcgtttggg cgatgtgatg caacaagagc atgcagatat gggtatctct
      181 ttttgtggta gtggtggtgc aggtgcatta acagcagcaa caaaatatgg ctatccagaa
      241 cgacatggta tgcgttcgat tgaagaaggg atcacagcta tcaaggatgg caaaactgtt
      301 ctaggtttcg gctttatgga tcaagaggaa ttagggaaac gtctgacaga agaatttatc
      361 aaaaagcatg gaaaatagaa agagggacat gaaatgagcg ttcaaaagac aattcagcaa
      421 aaagttatcg tctccggtaa aggggaaaca aagcaacatg cttttgcatc agcgttagca
      481 gaaattcaaa acaaaataat ggaacaatca aacgtattat tgcgaatcga accagtcaat
      541 gtggatattt taaaggcggt cgaaaacaaa tacacagaac gctttctatt tttcttcttc
      601 ccacggcaac gtattagcta tgaagtcgaa ctctctgtta ctgtggctgt aacagaaata
      661 aaattggcag acatatcgtt cagccagcaa atgactgaag atccaaatgg gattgccatt
      721 ccatttgtaa ctaaaaaaat ctaattttga atcataaggg ggaaagaata acgtggattt
      781 cgtaatcatt ctagtcaaat cggtcattat cgggggatta cttggttttg cagcatctgt
      841 cggggcagct cgaatgtttc atgcacctaa tacacaaggt ttaggagctt ttagaacatt
      901 aggcgaaatg aatgcctgca tgggtgatcc agcttctcat ttttcatttg gtttaggctt
      961 tttctttaat gcatgggctt cttcagttgg tgctggtgca ttcacacaag atgtgacgca
     1021 tcgaatcatt cccaattggg cagcggcagc acttctagca aaaaacaaag atgtcaccca
     1081 gaccatgcat gatccgaaaa aaatggggat cattggagcg gttatcgggg cagtggtggt
     1141 agccttctta aacacaacag ctgcagcaat tccagaggcg ttacaagtga cggcggtttc
     1201 cgttttggta ccagcagcga cgattctgat caatacagta atgccagtta ttttttggtt
     1261 ggcagcttta gacgcaggga aacgaactgg tttttggggc actctttttg gtggtatcgc
     1321 gcaattgatt atgggaaatg cggtacctgg tgttgttcta ggaatcttga ttggtaaagg
     1381 cgtcgatgag agcggttgga atcgcgtcac gaagagcatg atgatcgcta ttgttttatt
     1441 atttattttc agtggatttt tccgtggatt tgatatggaa atgatcaaga gcttccagct
     1501 tcaagtgcct aattggcttg ataacttgca caacatcttt agtgtgaaat aggaggagaa
     1561 tcatggagac aacagaagaa caagcaaaag tattagaagc aaagcggcaa aagagctttt
     1621 ggtttgcaga ctggtctttt ccgctaatcg taggaattat gtccgctgct gtatttgcag
     1681 gaacacacat gtatgtgatg tatggtgtgg gtgcattcaa tgaagtttcg atcgtggcga
     1741 tgttaaaagc cgggctggat ggcggatcct atggcgcagc ggcagcgttt ggtgctagtt
     1801 tcttatttgc cagaatttta gaaggctctt tagttgggat cttggactta ggcggttcga
     1861 tcctaactgg gatcggaatt ggggtaccag caattctctt aagtatgaaa atagcggcac
     1921 caatcgataa ttttgcactc tctttattga ctggtttggt tctaggtttg attgttggtg
     1981 gcatcattgt cttgattcgt aaatttacga tcaatcaatc aaattccact tttggtgctg
     2041 acgtcatgat gggggcagga aattcatctg gacgtttttt gggtccatta attatattaa
     2101 gcgcgtgtgc agcatctatt ccaatcggca ttggcgctac attaggagca ctaggattct
     2161 atgcttggaa aaaaccaatt gctggaggtg ctattttagg agcaatcttc ccggcagtaa
     2221 attaatagaa agagggtcat taaatgtttg atactttgat taaaaatgga aggttagttg
     2281 atggatctaa aatagaggtc gctatagaaa aaggcatgat taaagcagtt gcagcagaaa
     2341 taaatcagcc ggcgcaagaa gtagttgact tggagggaaa gtattattta tccgctgggt
     2401 ggatcgatga tcatgtccac tgctatgaaa agatgaatct ttattatgac tatcctgatc
     2461 agataggggt cgaaaaaggt gtgacgacgg tcattgatgc tggaactacg ggagcagaaa
     2521 acatccgtga attttatcaa ttgacgaaat cggtcaagac aaatgtgtat gcgctaatga
     2581 atatctctaa atggggcatt gtcaaacaag atgaacttgc tgatctaaca aagattcaag
     2641 aagatctagt aagtactgcg ttagcagaac tgccggaatt tattgtcggg attaaagcga
     2701 gaatgagtaa gacagttgtt ggtgaaaatg gcattaagcc attagaatta gctaaaaaaa
     2761 tccagacaga aaacaatgat ctgccattga tggttcatat cggttctgca cctcctgagt
     2821 tagaagaagt attgtcaaga atggaaagag gcgatgtatt gacccattgt tttaatggaa
     2881 aaccaaacgg tattttagat ccaaagacag atcaaattaa aaactttgtc tgggatgctt
     2941 acaataatgg tgtcgtcttt gatattggac atgggacgga tagctttaat ttccatgtgg
     3001 cagaaaccgc actaaaagca ggaatgaaag ctacctctat tagtacggat atctacatcc
     3061 gcaatcgtac aaatggccca gtttttgatc tagcaactac tttagaaaaa ttgagggtag
     3121 tcggctattc ttgggaagaa atcattgaaa aagttacagc agttcctgca cggaattttc
     3181 atttgaagaa taagggccag ttagctgtca attatgatgc agatttgacg atatttgaga
     3241 ttgttgaaaa tgaaaaggaa ttgaaagatt ccaacggatt tacacgtata gctaaagaac
     3301 aaattttacc gattaaaaca attattggag gcgtcatata tgacaattag ctatggaaag
     3361 tttgatttaa aagaagtcat taatgcatcg ggaaaaatga cgatcctagg ggtttctaag
     3421 gtatctgaaa gtgtcctagc agcacaacga tttggaggcg agcatttttt tgagatgagt
     3481 gatcttgcca tcaaaaccgg cgcttatctt tctcaattac tgaaagtgga agatgcgcaa
     3541 atcgtttctt ctgcttcagc agggattgct caatctgttg ctgctttaat cggacagggc
     3601 gacatgtacc atgtctatca tccctataca gaaaaaatcg ctagacggga aatcatcata
     3661 cctaagggac ataatgtaga ttatgggaca ccggttgaag taatggttgc tcaaggtgga
     3721 ggaaaagtca tagaagctgg ctatgcaaat atgtgtacac cggaacatgt ttccatgatg
     3781 attacggaaa aaactgctgc gcttctatat attaaaagtc atcatgcagt tcagaaaagt
     3841 atgctgacgg tggcagaaat ggtagaagtt gccaaagctc atcgtttgcc attgatcgtt
     3901 gatgcagctg cggaagaaga tttattcaaa tatagcgaaa tgggcgttga cttagtgatt
     3961 tactctgggg caaaagcaat cgaaggtccg agtgccggac tggtcatagg aaaaaagaag
     4021 tatattcaat ggattcggct acagtctaaa ggtattggcc gagcaatgaa aatcggtaaa
     4081 gataatattt taggctttac ccaggcacta gaagattatt taaaaaatgg gagcgaatcc
     4141 ggtgactcca tgaagcaacg attagcgcct tttgttaacg caatcaatca acttcctgac
     4201 ctttcagcga aaatagttca agatggtgcc ggcagagata tttatcgagc aagtgtaacg
     4261 attaatggcg ataaaagtgc gaaagaagtc attcaggaat taaaagcacg aaatccagca
     4321 gtctacaccc gagaatatca agcaaataac ggaatcattg agttcgacat tcgttccgtc
     4381 agccaaaacg aaatggaaaa aattataaaa agattgagag aaatcatgta ggaggctttt
     4441 aaattgtcag ttatcccaaa ttattataaa aatcgtgtat gtttaaatgt attagctcat
     4501 tcagttgaaa atgcaaaagc atgctatgaa gcagcggaag ggcatgtcgt actaggtgtg
     4561 ctatcgaaaa attatgctag cgatgaagca gcaatcgaag atatgaaaag gtaccaagac
     4621 gcaaccaata atgcgttgtc agttggatta ggcgctggag atccaaacca aagtcagatg
     4681 gtttcgcgtt tatctggggt tttacaacca caacatgtca atcaagtatt tactggcgtg
     4741 gggacttctc gtgctttatt gggccaaaat gatacagtca tcaatggatt ggtttcacca
     4801 acaggtaaga tcggctatgt caatattgct acaggtccgt taagttcaca agcgccagca
     4861 gctgaagtgc ctattgaaac tgccattcaa atgctgcaag acatgggagg tacttcaatc
     4921 aagtatttcc cgatgaaagg cttagcacat agggaagaat ttaaagcagt tgcggaagct
     4981 tgcgcaaaat atgaattcta tttagagcca acaggaggaa ttgatctttc aaactttgaa
     5041 gaaattctga aaattgcaat cgatgcaggt gtaaaaaaag tgattcctca tgtttatagt
     5101 tcaattatag ataaagaaac aggagatacg cgtataggag acgtaaagat actattaagc
     5161 atggtaaaaa aaattattca ctaactcatt agaaaataag aagcttattc cagaatgaaa
     5221 ttaaatggaa taagcttttt ataattaatt acggaaatt
//
LOCUS       DAYYME010000153         5250 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000153 DAYYME010000000
VERSION     DAYYME010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.154"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1273..1938)
                     /locus_tag="ACHBFW_002533"
     CDS             complement(1273..1938)
                     /locus_tag="ACHBFW_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9318797.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="ACHBFW_002534"
     CDS             2064..4100
                     /locus_tag="ACHBFW_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFM9318798.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="ACHBFW_002535"
     CDS             4104..4742
                     /locus_tag="ACHBFW_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9318799.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="ACHBFW_002536"
     CDS             4770..5198
                     /locus_tag="ACHBFW_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318800.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
BASE COUNT         1732 a          803 c          959 g         1756 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa
//
LOCUS       DAYYME010000154         5243 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000154 DAYYME010000000
VERSION     DAYYME010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.155"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..513)
                     /locus_tag="ACHBFW_002537"
     CDS             complement(55..513)
                     /locus_tag="ACHBFW_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM9318801.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(526..1857)
                     /locus_tag="ACHBFW_002538"
     CDS             complement(526..1857)
                     /locus_tag="ACHBFW_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFM9318802.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1868..3310)
                     /locus_tag="ACHBFW_002539"
     CDS             complement(1868..3310)
                     /locus_tag="ACHBFW_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9318803.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3297..4061)
                     /locus_tag="ACHBFW_002540"
     CDS             complement(3297..4061)
                     /locus_tag="ACHBFW_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFM9318804.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4165..5040)
                     /locus_tag="ACHBFW_002541"
     CDS             complement(4165..5040)
                     /locus_tag="ACHBFW_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9318805.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         1591 a         1103 c          764 g         1785 t
ORIGIN      
        1 aattttgtaa attcacccat aaatatcacc ctatttaact tttttcatcg tacactaaaa
       61 ttcaaaacaa ctatctttaa tctttacttc ttggttttgt tttaataaat ttccttcaaa
      121 aatgcctgga gaaactacaa tgatcaccgt atctttttgt gcttgctcca acttcttcaa
      181 atctataata gctaacagtg tcccttcttt tacctcctgg ttactttcaa cccgtatctg
      241 aaacggactt ccttcgagtt caactgtatc taaacccata tggatcagta tttttctgcc
      301 agattcactt tcgatcgtta tggcatgtcg ggtaggaaat atattatcga ttattccttt
      361 aactggcgca tggatatatc catcatgatt cgttatagcg tatccaactc ctatcatttt
      421 tgaagaaaag acctcgtctt ttactgctgt taatggaata atttgaccac tagctacagc
      481 ttttagaatg tttcttgttt taaagaaatg cactttttat cacctctact ttaatgctgg
      541 ccaatatttt ttgtttactt tgatcatttc atctagtatt ttttttgcga ctctggcatt
      601 tggtacggtt tgattcaatg taaatgcttg taaagctttt tgataagaac cttcaaagta
      661 agcatctact agtaacttct ctactgcaac ttgtgcctcc atcaatcctt tatgaaaatc
      721 actgattggt tttcttagtg taattggctc cacaccagtt gcaccaacat atgccggtat
      781 ttcaacaact gcatctgacc gtagattcgg aatcgcccct tgattaggaa cgattagcat
      841 aaagcgacga cgatcatcat tcaacaagga aatagctata tcgacgatat attgtccatg
      901 ctccccaaaa ttaaagttca atacttcttc cacaataccg cggcgtattt tatcagccat
      961 ttctttggta tttttctctc gtccatccat gaccatatta gcacgtgtat actctttgtc
     1021 agtatgctca acgaccatat caggatataa gtaatactct aagtaattat tcgggatatt
     1081 acttggaaaa tttttcgtga tgactgacat catttgaaag gttttttgcc aagttttatc
     1141 tcctatatta aaatcggcga cttgcatttc ttttgttatt agtttttcaa taatctctgg
     1201 cataatttct cgttgcaatt ctttatcata aatcgatgta taccagccaa agtggtttaa
     1261 accataatac gtaacgatcc aattcttacg atcataaccg taattcactg caatcgtttc
     1321 ttcaattgaa atagtcatat cacaggcatt aatcattttt acattcggat atttacgacg
     1381 aacagcttca gaaacgatcg actctggatt tgtataattc aaaatccatg ctttgggggc
     1441 atagtcttgt atatgatcga caatttccaa taaacctttg attgagcgca tcccataagc
     1501 aaatcctccg agtccacacg tttcttgtcc tactaatcca tgtttaagtg gaatcttttc
     1561 gtccttttct cgcatctcca atcctccaac acgaatttgt gagaaaacaa aatcgcatcc
     1621 tgtaaaagca agctttggat catccgttgc acgaatgcga atatttgaaa aaccttttct
     1681 tttcagcata aatttaacaa tcaagtacat atccatattt ctttcttcat caatatcata
     1741 taaacgtatt tcagataatg gcaaccgttt ttcattgttc aaaatggctt gaacgattcc
     1801 tggtgtatac gtactcccgc ctccagctat agtgataatc tgttttttca tcatcatctt
     1861 ttcctttcta ttcttctaat aatttattga attctgccat aacataagtc acttctaagc
     1921 cgatgataat ttgataattt ttttcagttg gtttaatgat cccgcttatc cctaattgct
     1981 tgattcgctg ttcctcgacc atggtgggat ccactacctc tacccgtagg cgtgtattac
     2041 aactatagac atccaggata ttggcttttc ctccaaacgc ttgtagcatt tttgttgcca
     2101 tatattcata atttttactt gctaacccta actctgtttc ttctaaggtt acttcattgc
     2161 caaactctgc cttagcttca cgtcctaatg tctgaatgtt tctcttattg atcaaataat
     2221 aaaaagtaac gtaatacaag gcaaaaaaga aaattccgat tggaagaatc aaaatagcat
     2281 tggtagcgat tcgaaaattg atgagataat caacaataca tgctccgaat gtaaaaccaa
     2341 ttcgtatatt gaataagtaa cagacaatcc ctgctaaacc ggcaaaaagt gcatggatca
     2401 cgtacaacat aggagaaaga aacatgaaac taaattcaac aggctcagtg atatttgcaa
     2461 taaatgaagt tcctgcaccg cttcccatga ttcctttgac catctcttta ttttctttat
     2521 aagctgcttt atagatagct agacatatag ctggtatacc aaacatcatt actacaaaaa
     2581 aaactgataa aaacagtcca gcctgaggat ctccgttgat aaagcgtgtc atctctcctc
     2641 tgaaaacttc cttgctagga gatgtatagc ttcctaaatc gtaataaata tacgtattca
     2701 acacatggtg cagtccaaag gggatcagca aacgattcaa aaacataaat atccccacac
     2761 ctaatgctcc catatcaacg agcagttttg caaaattatc aatcccaatt tggatgagcg
     2821 gccagataaa gcccaaaaaa acagctgcga ttgtttgtag aatcatcacc atagtcaatg
     2881 ggaatttttc gcccgcaaaa taagaaaaaa ctactggtaa tttttgttct ttaaagcgat
     2941 tatacgtcca tgccgctact aaaccggaaa taattccacc aaaaatcccc atattcactt
     3001 ctgggtccat aatttctaag ccttttttca aggtagcaat cgctaaaaaa ccagtaagtg
     3061 caggtattcc tttgtcctta tttttgacaa aaccgattgc aactcctata gcaaataaga
     3121 tatccaagtt tccaaaaacg gcatttccag ctgcaccaat tactgggatg ttcagcatat
     3181 cgtcagctga taaacgcatg atcaatcccg ctaatggcat cgctgcaact ggaagtagca
     3241 tcgctcttcc aagcttctgc acatttgatt taacattcat ttttattcct cctctattaa
     3301 atatcctcat atgttactaa ctcaatcaaa ggatcatttt tcactttttg agcaaatgct
     3361 gaatgaatta aaaattcgac ttggtgggta cgaggataca caaaacttga accatttaac
     3421 aattcctgat ccaaatacgc tgcgtgacac atgacttcga tcgttttatc ttctctcaat
     3481 atcatgagta aacttgccaa atattcttcc acactcgtat tgatttgtcg ttttttttca
     3541 tcagtttctc ctacgacatc gaaacaaggc tcaaaataat cgacatgacg aacttcatct
     3601 tttcgttcaa agttgctccg aaccggcaaa tcatattttt ttgctaatgt aacaactacc
     3661 tcctgatttt catccaaagt atgagtatga tgatgactgt ctaaatgagt tggtatgatc
     3721 cccgcatgat aaattttttg aatctgagca ttccattcct gatacagttg atctttgtca
     3781 atgttaaatg gatgctgata ataagataag gatctgaact ctccgctttc gataagagta
     3841 tcgacagttt tgaggagtgg tttgccacac gtcaatgtta aatggactcc tacacctagc
     3901 ctaggcattt cctttcttag tttcacagca tgctcgaaac ccggcatatt tgccatcaat
     3961 gtagttgatg tcaaaattcc tcgctgataa gcatccataa tcccataatt cacaccatgg
     4021 ctatagccaa aatcatccga attaaaaatc acttttccca tttcattctc cttctttttt
     4081 ctactaaaat aggcgcaacc aaataatcat agtccattta ctatgtgtca tttggttgcg
     4141 cctatagata gttacgccct tattttaata gattcttgct agatgaattg ccaaatagat
     4201 gatttcattt tcttgaatgg caagttgaaa ttgctgttcg atgatatctt taatctttag
     4261 tgctgtacaa tacgctttgc tatgtttaaa tgtttcagta aaaaaaccaa tattttctac
     4321 tggcgtttct tcctgataaa tcaagcgttt aaccaggaat ttgatatgaa ccattaagcg
     4381 atgataagtc agtgattctt tatcataaac gtttgaatag gatttttcta gatgggacaa
     4441 aatcgtggtc gtcatttcta ctatttttaa gctctttaaa ctattaggct ctcctagctc
     4501 agcattaatg atatgcatag tgatgaatcc tacttcattt gaccctaact cggtttgata
     4561 tcgatgattg attcgctcga ccacttctac tgctatatca tattcatttg gataaaggaa
     4621 tctcatttct ggcaacataa ttgctgtgac atctctatct tctctttttc gttgaatagc
     4681 aaaataaata tgatctgtca atgtaatata gatatttgaa tctaatggag aggagatcct
     4741 agctgttatc agttcaatca cttcttccgc aaaatccata aattcgattg gaatctgcga
     4801 gactatctca ctgaatcgtt tgatcttttc tttagaatct aatacaaata atttgtcagc
     4861 atcctcaaca tcaaatggct tactcacttt agcagaaaag ccaatccctt tccccatcgc
     4921 aattttttct attccatcgt catcgatcaa tacagcatta tgattcagct ttttcatcac
     4981 atacaccgtt aattcacctc ctctatatga taaaacaaaa caacttgcag actccttcat
     5041 acatattttt gtctccaaac aatttttaaa gacaaaaaac gacctaagaa agaacccttt
     5101 cttaggtcgt gcctaattga atagtagcac cctacataat ttacaaaaat agtttaacac
     5161 ataataaaag cgttatcaat atctttttta ctcgttttat gataatactt gtatcgaatt
     5221 gatttggagg taattacata aaa
//
LOCUS       DAYYME010000155         5202 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000155 DAYYME010000000
VERSION     DAYYME010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.156"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(326..1195)
                     /gene="scrK"
                     /locus_tag="ACHBFW_002542"
     CDS             complement(326..1195)
                     /gene="scrK"
                     /locus_tag="ACHBFW_002542"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM9318806.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(1215..2336)
                     /locus_tag="ACHBFW_002543"
     CDS             complement(1215..2336)
                     /locus_tag="ACHBFW_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM9318807.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(2338..2733)
                     /locus_tag="ACHBFW_002544"
     CDS             complement(2338..2733)
                     /locus_tag="ACHBFW_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9318808.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(2752..3549)
                     /locus_tag="ACHBFW_002545"
     CDS             complement(2752..3549)
                     /locus_tag="ACHBFW_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9318809.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(3551..4339)
                     /locus_tag="ACHBFW_002546"
     CDS             complement(3551..4339)
                     /locus_tag="ACHBFW_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9318810.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(4353..4829)
                     /locus_tag="ACHBFW_002547"
     CDS             complement(4353..4829)
                     /locus_tag="ACHBFW_002547"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9318811.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(4901..>5202)
                     /locus_tag="ACHBFW_002548"
     CDS             complement(4901..>5202)
                     /locus_tag="ACHBFW_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9318812.1"
                     /translation="NPTKLLERVSDAIYILQNDLGISFDNNTCFGLYVHISCLIERLV
                     KQNTLEDEIYFNETSEEFQKFQTHFKQSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         1631 a         1130 c          767 g         1674 t
ORIGIN      
        1 ggaggtaatg gtctgaacac caagaattgt tccaacatcg aattgcttaa ctaatgagtt
       61 caaagcttaa ttatgtggct cttatattta ccgagcgtgg attgcgatct ctttagcgaa
      121 acaaacatct ttcataaaaa aaacagccat atccgctttc ttggatatga ctatttacta
      181 ctactttact atctttcatt cttcttgtct cacttatgat aaggcgctgg gtatcatgag
      241 tgaatgatat tgctgaatac aaatagtccg accacattaa gttgatcgga cttcataagc
      301 attttattga ctaatacttt tttgcttaag aattgattct aacatctttt gctaaagtta
      361 agcatcctat agttcctgca ttatctgcta acttaggtgt gacaatgtat tcttcgatat
      421 ttggcgtatc gacatagccg ttaattagtt ctaaaaaacg tctttgtact ttctttttca
      481 gatgttcttg ttgcatgaca ccaccaccaa aaattataat atctggtgaa agcatcaggg
      541 tcgtattaca ggcacattgt gcaatataat ccgcctcgat ttcccagaac ggatcttcta
      601 tagaaagatc ttgtcccttg gttcccgtac gcatctcgat agcgggtcca gatgccaacc
      661 cttcgagaca atcatgatga aaaggacaag taccagagaa agtatctcct gaatgtctct
      721 taactaacgt atgccccatt tctggatggc taaacccttc aacaaaaatc ccatcttgta
      781 tagctcctcc accaattcca gttccaacag tgtaataaac aacactggat aaaccttttc
      841 catgacctga aacatactcc ccgtaacaag cagcattcac atcggttgtc caggcaattg
      901 gaatttcgaa atgcttattc aacgttccaa caaaatcaaa atttttccag gcagtttttg
      961 gagtagaggt gatatgtcca taggtctttg attcacgatg gatatcaatc ggtccaaatg
     1021 accctactcc aatgctttct aattgttctt tatagtgatt aaagaaatca attaccaaac
     1081 tcattgtttc attcggtgta gttgtaggaa aactcactct ttctaaaacc tttaaatcat
     1141 cattgccaat ggcacaaaca aatttagtcc cacctgcttc tattgagcca tacatttctt
     1201 actccaccgt ccttttatct aggaattcgt tcttccctgt ctaatagttt ttcgaaaaac
     1261 tcatgtggtt ccttttggta ccaataggca acagaagaaa tatcgtcttg acgctcataa
     1321 agcttgatgt catcatttcc tagggtttgg aaagtaacac gtaaattttc tttgaaacta
     1381 atggggtcgg gtaaatgcca gcggtacaac ccatgcatcg gtaaagcatc atcgccaaaa
     1441 ccatgaactg gatttggatt aggttttcca gcactaaaat aatcccgggt gtgatctctt
     1501 ttagattgaa aaggatagcc cataaatagc gtattaaaat tttgagcata tgccctttct
     1561 ccaaagttct ttttatggaa agcccaagcg ccgccaaaat aatcttctgc tcctgtagat
     1621 gttattgtag ggaaatcttc atctccatct atataaaact taaattctcc ttccccccac
     1681 cagtatcttt ccaatgcgca caaagcaaag taggttccta cataataccc agtgcctttg
     1741 attccatcaa taagggtgta atcttttcct tttgtcgtga tgggttcacg gttccattta
     1801 gcatgaaaat acatcgcatc atccggtact tcagtcaatg cataattaaa tgtataaaaa
     1861 atgtgcttaa taaactcagg atgttcattc gttaaagtaa tcttagcttt ttttcgaaat
     1921 ggcatctcaa aatagcaatt catccctgat gttggattca ctacgattgg taaggaattc
     1981 acttcgcatc ttgtcccaaa cccattacaa aaaaaatctc ctaatggact ttctacagaa
     2041 ggagtggatt catcatccca ataaatacgc aaaattaaat cacgtaagac gaaactccca
     2101 gcgattgttt tgtctgtcac tgtcatccaa aaatgacgta aaattccagg gccttccatt
     2161 tctgctaaag taattgtctc tccagcattt atcggaacag aaggtgaccc ttttctccca
     2221 attcctagat gactcgctgt cattgccgct ttcccttttt caccattttt attttctggt
     2281 gaaatagttc tactttcctc tcccttgtgg agccatatat tatttaaaca attcatttta
     2341 ttcgtcctct atcctttctt cacttttttg attattcata tagatgattt gttgttgtgc
     2401 ctccgcaata attgctccaa tatctaaatt atcatttcct aactgcaaag cgattgacat
     2461 tgccaaggga agattcattc ctgcaattac gtgcactctg tcagaaatat aggtaaaaaa
     2521 cttttgattt acacttcctc cagccatgtc agttaaaaca attatttcgt ctgtagtatc
     2581 cactaactct ttaatttgtt catctatatc atcaatttct tcgacatatg cacataagct
     2641 actaatttga ttatgcatac ctgttaaaaa ctctagtgtc tctttaaaac cctcagctag
     2701 tattgaatgg ctagcaatga tcaatcttct cataattcaa atccttttcc tttacgctaa
     2761 tattccaaat gcagccataa ccatcgcaaa aacaataacg agtagaatca aaatggtcat
     2821 actgattttt ttcttactga gtagataata tgctgcagca gtaataatta ctggaattaa
     2881 tgttggtaaa attttgtcca acattggttg gattttcata ctaacttcac caaaagtgaa
     2941 tttaagtggc gttgaaacac tgataactga agcaattaat gatccaacta ctgttagccc
     3001 caatactgaa gcagcttcag ttaaataatt aatcttttca ccgtattcag taacaatctt
     3061 cattccctgc ttataaccaa attcaaagcc tctcattcgg aaaaagaaga ttaccatgat
     3121 taaaatgaac cataaaaaga ttcctacagg attattttct aaagccatat aagccgcaat
     3181 tgatcccatg atggttggga tcatcgtcat caaaagagag tccccaatac cagcaattgg
     3241 acccatcgta ccaactttaa agtcattaat cgcatctttt gcttctaatc cttgcttttc
     3301 ctccattgca acacaagcac ctaaaataat ggctgccagc caaggctgtg tattgtaata
     3361 ctgaaagtga ttatttaaag cttcgatgta ttcatcatca tctttgtata attttcttaa
     3421 aataggtgac aatgcgtaag tcacagatgc accttgctgt gtttgataat taaaggtaca
     3481 aactgacatc aaccaacgat agttcgcttt cctaagatcc tttttcgtta cttgatattt
     3541 ttcgctcatt ttattcatcc tccccaataa acccaacatc agaagctccc gcagaagcag
     3601 tgatttgtgc atctttttca gctctatgat acacattaaa tgctaaagcg aatcctatga
     3661 tcgccgctcc aataataggt accttcaaaa aagcggctaa gaaaaatcca acaagtaaat
     3721 aactaatgta tcgacctgta ggcataaagc gtaacaacat cgcaatacca acagcaggca
     3781 acatttttcc ggctaatgtt agcccatcca taaaccaatt aggtaaaaca tccaaaacgt
     3841 aattaacagc agactgtcct aaagtaacgg caataaatac tggaattgct gcagataaac
     3901 caaagaaaat tggccccaac cacaaaacac tattcatttt cttaaattct cgtttattcg
     3961 catattcctg tgatttgcga acaacaaaac catttaagat tttgacaatt acatctagtt
     4021 gcacccctaa catacctaca ggtagtccta ttgctaatcc ggcttctact ccaagctggc
     4081 taccaaatgc cgttgcaata attgcgcacg ttccatagtc cggtacagat gagccaccaa
     4141 aaccggcaac tcctaatgac atcaattgaa ctgtaccgcc aactatcaaa cctgttttta
     4201 tatcacccat caaaattcct gtgaataatc cccaaaaaac tgcgtaattt acaaagatca
     4261 tcggaatccc ataataatca acatgtttaa taaatgctaa tatagtcagc ataataattt
     4321 gaaaaatagt aaaatgcatt atccttactc ctttatccta attttgattg aatttcctgt
     4381 gccttatccg ccggaacaaa ttgtgtaaca atttctacac cagaatccaa caatttttta
     4441 tacgtgctga tattttcatc tgatgcatag gctctagaag atagctgtaa tgcattatct
     4501 ttaacaacag taccgccaac aattagtcgg tcaattctaa taccgttatc tactaaatga
     4561 aggattgttt ccggctcttt cacgatgaca aatacttttt cctgatcgta tttccccttt
     4621 ttaaaattcg caatcgctgt ttcttcagtg ataattgata cagccattcc agcaggtcta
     4681 gctaaacgca tacttgattt ttggatgtcg ttatttgcga cagaatcatc gatcaccatc
     4741 actctgtttg ctttagacat tggtgcccat tgcgtgacaa taatcccatg caataaacgg
     4801 ttatcaactc tcgacataac aatactcata aatacttcct cctttatctt tcattttctt
     4861 acaagcaatt accatgccaa aacaaaaaga acttgataaa tcaagctctt tttacataat
     4921 cataaacgta tttaacctca tgaatcggga tgtcgacact ataataatgt tcaacgacac
     4981 tgaaagattg cttaaaatgt gtctgaaatt tttgaaattc ctcactcgtt tcattgaaat
     5041 aaatttcatc ttccaaagta ttttgtttta ctaatcgttc aattaagcaa ctaatgtgaa
     5101 catataatcc gaaacacgta ttattatcaa atgaaattcc tagatcattt tgtaagatgt
     5161 atatagcatc tgaaacccgt tctaataatt tagtcggatt ta
//
LOCUS       DAYYME010000156         5143 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000156 DAYYME010000000
VERSION     DAYYME010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.157"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..1843
                     /locus_tag="ACHBFW_002549"
     CDS             212..1843
                     /locus_tag="ACHBFW_002549"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM9318813.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1846..2925
                     /gene="uxuA"
                     /locus_tag="ACHBFW_002550"
     CDS             1846..2925
                     /gene="uxuA"
                     /locus_tag="ACHBFW_002550"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM9318814.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2999..4426
                     /gene="uxaC"
                     /locus_tag="ACHBFW_002551"
     CDS             2999..4426
                     /gene="uxaC"
                     /locus_tag="ACHBFW_002551"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM9318815.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            4510..>5143
                     /locus_tag="ACHBFW_002552"
     CDS             4510..>5143
                     /locus_tag="ACHBFW_002552"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFM9318816.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYE"
BASE COUNT         1784 a          702 c         1079 g         1578 t
ORIGIN      
        1 aataaaagaa tacatgaaaa aaagaaaaaa taagtttcga taaaatcagt ccaaaaagaa
       61 ggagtatttt atttttctga ataaaaaata caatcattct gatatggtag aaaaaagcaa
      121 gtttattccc tttttatatt gacaacggtt acaatgggtt ttaaaatata aaatgtatca
      181 aaactaacat atcagtttga ggaggttaat tatggtttcg attaaaaatt ggcaaaattc
      241 tatcgaagaa ttcaatgaaa tgcagattct actgccgtgt tttgatgtca aagaagttaa
      301 gcaagaaggc cagcaacatc cagtatggct tcattttggt ggaggaaatc tttatcgagg
      361 atttcatgca gaaattgctc aatgcttagc agatcaaaat gaattagaaa caggagtagt
      421 tgtgtgtgaa acatttgatg aagaagtagt ggaaaaagct tatcatgact ataacaatga
      481 cattttggaa attgttatga gtggagatgg tgaactcaaa aaaagacttt tggcagtaac
      541 ggctgatagt ttatattgca atccgaagaa tatagcggac tatcaaagag tggttaaata
      601 ttttgaagat cctaaattac agtttacaac gtttactatt actgaaaaag gttacagttt
      661 aaaagattct aaagggcaat tcttttcggt agtcaaagct gatatcaaat ctggacctag
      721 caatcctcaa catacgatga gtattgtaac atccttgtta tatagcagat ttaatgctgg
      781 agagttgccg attgcgatgg tttctactga taatttctca cagaatggtc aacgttttca
      841 agatagtatt tttacgattg cagaagcttg gcaaaaagaa ggatatgttt ctgaaaaatt
      901 tattgcttat ttaaaggata aagaaaaagt gacttttcct tggagtatga ttgatcgaat
      961 tacgccaaat ccttcagaga atgtaaagga agatctattg atgatggggc tcgaggattt
     1021 agatatcatt cacacaaaaa aacataccaa tattgctgct ttcactaata ctgaggtagt
     1081 tcattattta gttattgaag atagttttcc aaatggccgg cctaaattgg aaaaggctgg
     1141 tgtgattatg acagatagag agacggttga taaagcagac atcatgaagg ttacgacaag
     1201 cttgaatcca cttcatactg ctttggcagt aacaggttgt ctgctaggat atcaatcaat
     1261 ttcagcagag atgcaggatg atgatttggt tggtttaatc aaaggaatag gttatttaga
     1321 aggtttacca gtagttgaaa atccaggcat tattgatcca aaacaattta ttgatgaagt
     1381 aattaataaa cgtttgccta atccctatat tccagataca ccacaacgaa ttgcaacaga
     1441 tacttctcaa aaagtagcga ttcgctatgg tgaaacaatt aaaaagtatg tcgaaaaaga
     1501 cggaaatgca gaaaatctaa catttattcc tatcgctatt gctgcttggt tacgttattt
     1561 actggcagtc gatgatcaag gggaaaattt tgtgccaagt cctgatccgt tattggtaga
     1621 gttgcaggat aaattatcag tattgaaatt aggtgaacaa gatccttcaa agattcattg
     1681 tgcggtacag gaaattttag aaaataaaga aatctttggt ttagatttat atcaagttgg
     1741 ggtaggaaag aaaactgaaa aaatcttttg cgaaatgcta gtaggtcctc aagcagttcg
     1801 taaaacaatc aataaatata taacagaagt tggaggaaag taactatgga aatgactttt
     1861 agatggtatg gggcaaatga agaaaaaatc tcactacaag aaattcgtca gatcccagca
     1921 atgaaaggaa ttgttggaac attaatggat ataccagccg gagaggtttg gccgagagaa
     1981 cgaattaaat ctttaaaaga tgaagtcgaa gccaatggac ttaccttgaa agttattgag
     2041 agtgttaatg ttcatgatga tatcaaaatt ggtttaccaa cacgagatca atacattgag
     2101 aattataaac agacgattcg caatttggca gaatttggga ttgaggttat ctgttataat
     2161 tttatgccag tatttgattg ggtaaaatcg gatttggatt atcgtttaga tgatgggtct
     2221 tctacgttag cctatatttc aaaaaatatc cctactgatc cggaggaaat tattagagaa
     2281 gtagaaaatt ctactaataa ttttgaattg ccgggttggg aacctcatcg gttatcacaa
     2341 attaagcaat tattagaagc ctataaagaa gttgatgaag agaaattacg agagaacttt
     2401 gcatattttc tcaaagcaat tatcccgaca tgtgaagaag ttgggattaa aatggcagtt
     2461 cacccagatg atccaccata ttccattttc ggattacctc gtattgttaa aaatcgtgac
     2521 gatttagatt ggttgtgtaa tgtagtggat tctccttcta atggtattac attatgtact
     2581 ggaagtattg cagaacgacc agataatgat gtctatgaaa ttctatctga attttgtcaa
     2641 cgggatcgga ttccttttgc acatgttcgt aatatcaaat atttaagtga caatggactc
     2701 gatttttacg aagcgccgca taaatcagaa tatggttctc tagatatgta taagatatta
     2761 aaaacaatgt acgataatgg atttaatggc tatattcgtc cagatcatgg tcggatgatt
     2821 tggggagaaa ccggtagacc tggttatgga ttatatgacc gagctctagg tgcagtttat
     2881 ttaaatggta tttgggaagc gcttgaaaag ggggcgagta aataattata taagggagtt
     2941 tatttagagg aaaaattttc tcttctaaaa actaacggaa ggaaaggagt gtttaaatat
     3001 gtttttagat aagaactttt tattagagaa tgaaatagct aaaagacttt ttcataaatt
     3061 tgcaaaacac caaccaatta tcgactatca ttgtcattta gacccagcat atttgctaga
     3121 aaataagcaa tacaagaatt tgactcaggt atggttaaat gataaaggag ctggagatca
     3181 ttataaatgg cgattgatgc gagctaatgg tatagaagaa gagttgatca gtggtccagg
     3241 tgatgattat gaaaaatttt tagcttttgt aaaaacagtg gagaaggcgc caggtaatcc
     3301 tatttttgaa tggtctcatt tggaattaca acgttacttt gatattgatt tgacaatttc
     3361 tgagaagaat gcaccagaaa tttggcgacg tgctaacgaa aaaattcaaa cggattccta
     3421 tcgcccgaaa gaattgttgg agatgatgga tgtgaaagcc atctgtacaa cggatgatcc
     3481 tacagatgac ttgtggtatc atactgagct ttctaaacag gataattctt tcaaggtatt
     3541 acctactttc cgtccagata aggtctggag tatcaaatca tcaggctttt cgaactatat
     3601 tttaaaactt agtcaaacag cggctatacc aatcaaagac tttaatgggt tgaagcaagc
     3661 attgagccaa cgagtggatc attttcatca aattggctgt cgattagcag atcacggttt
     3721 gaatacttat ttttatgaaa tagcagatga aaaaatactc gacaatctgt taataaaagc
     3781 tttagcagga aaaacggatt tccatttatc agaaattgca caatttgtta caggtattca
     3841 attacatttg atgtctgagt atacaagata tggttggacg atgcaaatgc atttaaatgc
     3901 tttaagaaat aattcaagta tattaaagga acaaattgga gtagatgtcg gtggtgattc
     3961 gatgggggat caagctagtt tgacacaaaa tgttgtagca ttgttcgata ccgctgaaaa
     4021 gagtgggtgc ttaccaaaaa caattttata ttcattaaat ccaactgatt ggcttccact
     4081 agcaacagca atgcaaagct tccaaggggg tatgattcaa aaaatccaat tgggatgtgc
     4141 ttggtggttc aatgatacag ctgaaggaat gcgtcaacag ttgactacga tggcacaaca
     4201 aagtttgttg tcgaatttta caggtatgtt gactgattct cgaagttttc taagctatcc
     4261 tcgccatgag tattttcgtc gagtattatg tcaattacta agcgagtggg ttaaagcagg
     4321 ccgaattcca gatgatgaaa tgtatcttag tcagatagtg gagaatattt gttatcgtaa
     4381 tgcgtataat tattttaatt tttttgaaat cggaagtaga aaataatact tgaaataaaa
     4441 gcttaaattt attctttgta agttagtaca gctagacaaa gtatgtatcc ataaaggaag
     4501 gagatagata tgaaaaaagt agaaatttta agtaagttag aagcggctgg agtcattgcg
     4561 gttgtacgtg ggaaaaataa ggatgaggcc attaaagcaa taaattcaat tattgcgggt
     4621 ggaattaaag ggattgaact aacttttaca gttcctaatg ctgaagaaat aatcaaagaa
     4681 attgctaaaa aatatgctga tcaaaaagat gtaataatcg gagccggtac ggttttagat
     4741 gcgtttactg ctcgtatcgc aattatggct ggggcagaat atatcgttag tccatctttt
     4801 gatcgacaaa cagctgagat ttgtaattta tatcaaattc cttatttacc aggttgtatg
     4861 acaattactg agataaagga ggcacttact gctggcgtag atatcattaa actattccca
     4921 ggttctgctt ttggtccatc cattgtaaaa gcctatcaag ctccattacc acacgtaaat
     4981 ataatgccta ctggcggagt aagtatagag aacttagaag attggtttga agtaggagtt
     5041 gttgcagttg gtgtaggtgg gaatttattg gcttctgttg aaaaaggtga ttttgaagaa
     5101 gtaagtcgag tagctagtaa atatatagat aagttttatg aga
//
LOCUS       DAYYME010000157         5090 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000157 DAYYME010000000
VERSION     DAYYME010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.158"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            63..1997
                     /locus_tag="ACHBFW_002553"
     CDS             63..1997
                     /locus_tag="ACHBFW_002553"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM9318817.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            2454..2870
                     /locus_tag="ACHBFW_002554"
     CDS             2454..2870
                     /locus_tag="ACHBFW_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM9318818.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            2884..3450
                     /locus_tag="ACHBFW_002555"
     CDS             2884..3450
                     /locus_tag="ACHBFW_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFM9318819.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            3473..4459
                     /locus_tag="ACHBFW_002556"
     CDS             3473..4459
                     /locus_tag="ACHBFW_002556"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM9318820.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(4614..>5090)
                     /gene="scm"
                     /locus_tag="ACHBFW_002557"
     CDS             complement(4614..>5090)
                     /gene="scm"
                     /locus_tag="ACHBFW_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM9318821.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         1540 a          942 c         1122 g         1486 t
ORIGIN      
        1 atcaaactaa aagaattatc atatattcat tatatagata gtctataagg agcggatgat
       61 caatggcaaa gcaaaatatt gtcgttgtag gagcaggata tgccggtgtt tcagctacaa
      121 aatacttggc aaagaaactg aaaaaagaag atgtagcaat tactctgatc gatcgtcatt
      181 cgtaccatac tatgatgacc gagttgcatg aagtagctgg cggacgtgta gaaccagaag
      241 caatccaata cgatctgcag catttgtttg ctcgtcaaaa gaatgtacaa cttgtcacag
      301 acactgtaat cggtatcgat aaagaacgaa aaatcgtcaa gaccaaatta ggttcctatc
      361 cttttgatca gctgatcctt ggaatgggcg gagagccgaa tgactttggt acacctggtg
      421 tcaaagcaca cggattcacc ctttggtcat ttgaagattc tgtaaggatt cgggaacata
      481 ttgagaagac agtagcaaaa gctgcattgg aaccagatga tgaactgcgt aaagccatgc
      541 tgactttcgt tgtctgtggt tctggattta ccggtattga aatgattggg gaactgatcg
      601 actggaaaaa tcgcctagcc aaagattata aattagatcc aaacgaaatc acactgatgg
      661 tcgtagaggc aatgccgacg atcttgaata tgttggatcg aaatgatgct gcaaaagctg
      721 aacgctattt gcgtaagcac aacgtggaat tattattgaa tgcaccaatc gtagaagtgg
      781 ctgctgatca tatcaaattg aaagacggac gcaacattcc gactcacaca ctgatttgga
      841 cagctggtgt caaaggaact tctgacgctg ctgactttgg attagaatca gcacgcggac
      901 agcggttgat cgccaatgaa tatatgcagg caaaaggcta cgaagataaa aacatctata
      961 tcattggtga tttagtcttt tatgaagaat tcccaggaac accgacaccg cagatcgttc
     1021 aagctgctga acaaacagga cacacagccg cagcgaatat cgtggcttcg atcaaaggcg
     1081 gagaaaagca taaatttaaa ggaaactatc aaggatttat ggtttctatt ggtgccaaat
     1141 ggggggtagc taacctattt gataagatcc atttaagcgg atttttagct atcgtaatga
     1201 aacatatcgt gaatttgaaa tacttctttg atattcgttc tggttattat atgttccaat
     1261 atttaatgca tgagtttttc catatcaaag atgatcgcaa cgtgactcga ggacattctt
     1321 ctcgttacgg caatgtctta tggagtgtgc cacttcgtat tttctatggt ttagtttggt
     1381 tcgttgaagc ttccaaaaaa atcattggga atggtgaagt gttcaaacca agtacttggt
     1441 ttggtgaagg atcatggttc actggaaaca gtgtgttccc ttggacatgg gaatacaatg
     1501 taacagatgt tgccagtggt gcttctgctg ctggtgatgc aaccacagct gccagcggag
     1561 caggggcaag tgccggagaa gcagcgactc aagctgcaca ttttggtttg agttatgcat
     1621 acggtgaaga accaatggct gtgattgaga agtcaccaga ctggttcaca aagatcatgc
     1681 aagtgatcat gccaaacaat gatgtagcaa tgttcttcca aaaattcatg gtattctttg
     1741 aaattgcctt ggcattagca ttgattgctg gattattcac atggctatgc agtgcgacaa
     1801 cgattgtatt ggtgatcacg ttctgcttat caggcatgtt ttactgggta aacatttggt
     1861 tcatttttgt tgctttcgca ttgatgaacg gttccggacg tgcgattggg ttggatcgct
     1921 gggtaattcc atggctacaa cgtacactag gtaattggtg gtacggtaga ccaagttccc
     1981 agtacggcaa gaaataaata gataaagaac gttatttgaa tgcaagattt caatagaaag
     2041 gggctgagac aaaccgtcac agcctccttt ttatgtaaaa ttaaaggaat aagatgcccg
     2101 agataagcct ccatgagcaa gacacaggtg tcacaacctc tgttacaacc tcttgttata
     2161 aacggtgttc cagaagtagc ttcttcgaaa aataagccga aatttcacaa aaacttgaaa
     2221 agtaattttc gtaaattcct tcttatttct tgaagttgcc cacttctgtc acaacctctt
     2281 gttataaacg gtgttccacc tgctgcttct cggtaataag cccaaagaat tagaaaacat
     2341 ggaaagctgt tttctattct tcgttcttat tactcaaagc aagacacagg tgtcacaacc
     2401 tcttgttttt atgctaaaat tgggtgagtt aatctggaaa aaggagaaca aaaatgagcc
     2461 ttaaagaatt tgctgaaaaa agtctgctca aaccttggga tgtttttatt attttgcttt
     2521 tgactgttct ttctttttta ccagtcatga tttttggtct tcagcaaaac accgcaacag
     2581 cagaaaaaga agcggtactg cgtgtggatg gcacagaaat caaaacattc cctttgatgg
     2641 ctggagaaga gagcaacaca tatacttaca aagatgaaca cggagattat aacttgatcg
     2701 agatagatgg cgatcgagta cggataaaag aagcagattg cgatgaccag atctgcgtac
     2761 gtcgaggctg ggcaacgaaa aatggcgaaa ccatcgtctg tttgccacat aaattagtga
     2821 tcgaagtccg ttcaacagat ggaggtgacg aagacagtct gatttattag accttcacac
     2881 accatgagta aattacaaaa aatgatttat atttcgctac ttgttgcaca aggagtgatc
     2941 attggtttat tggaaaatat gatcccttat cctttttctt ttgcgcctgg tgcaaaacta
     3001 ggcttagcca atctaatcac gatcgttgcc atttttacca tgaaaaaacg agacagcttc
     3061 ttactacttt ggctacgact attcctgact actttgctag gcggaacgat ttccacattc
     3121 ctttatagta tgagtggtgc tttgcttagt tattttggaa tgttattggt caagcaatta
     3181 ggaccaaaac gtgtcagtat catcggaatt agtgcggccg gcggtttcat gcataatgtc
     3241 ggacagcttt tgatggcttc tttcattgca caatcatgga cagtcatgtt gtacttgcca
     3301 gtcctttcag ttttagggat tttatcaggg attgcaatcg ggatcgcagc gaattatctt
     3361 ttacgacatg tgcagacact tcaacgattc caaaaagatt atgagcaggg aaccaaacaa
     3421 aaatggagcc caatgtttac caaaaaataa tcgtcacaca aggagttttt ttatgaagct
     3481 acatccaatg tgggaaaact atcctgcttt aagtaaagaa ttgacagaaa cactaaaacg
     3541 gatggaagaa actgtccgcc tgaaaaacaa acctgtcgaa caagcaatca aagaaacgat
     3601 ccatgccggc ggaaagctgt tgcgtcctgc ctatcagcta ttgttctcac aatttggacc
     3661 agagcaagat cgagacaaag ccatcgcttt ggcagcttct atcgaaatgc tccatacggc
     3721 cacactgatc catgatgata tcgttgatga agcggatctg cgacgtggtc agcctaatat
     3781 ccgtagtcag tttggtaatg tcgtttctgt ctatgcagga gactatttat ttgtctgctg
     3841 ttttaaacta ttgtctgatt actccacttc cttgaaaagt ctccaattga attctcgcag
     3901 tatggaaaaa gtattgaatg gcgaattagg gcagatggat gatcgctata attatgaact
     3961 ttctgtaaaa gaatatttgg aaaatatttc aggtaagaca gctgaactgt tccaattaag
     4021 ttgttctgtt ggcgcttttg aaagtggaac aagcgagcgc ttcgcaaaaa aagcaggaga
     4081 tatcggactt tctatcggga tggcctttca gattatcgat gatattttag attatacaaa
     4141 agagagccag gaaatcggca aacctgtact agaagacatg cgtcaagggg tctattcttt
     4201 accgttgatc tactctttgc aaaaaaacaa accaaagcta ctaccttatt tagagaagaa
     4261 agcggcaatg acagaagaag atgtcgacgc cgtacgcaaa atcgttgaac atacaggagg
     4321 cgtggaagaa gctaggaagt tagcggcctc ttacacagaa aaggctttga aggaaataaa
     4381 gaaattacct gccacctctc ttcgaacaaa agagaatctc ttttccctta cccaactgat
     4441 tttggatcgc aaagactgaa tagggaaagg ttcgatacgt aaataaattt ctcagaagga
     4501 aatctacgtg ttttttgaca aattgaattg tagttcttgt atttatgata aaaagcaaaa
     4561 ataaagagca acgtccgtta gcgacgttgc tcttttttat cttgaatatt tttttacctt
     4621 ttcgatttct tataactcat aaaagtgatt cttaaaccga ttacaagtaa tataacgcct
     4681 gcgagtgtta tccaattatt cgattgactt ccagttttag gaagtgctct ttttgatttg
     4741 tttccattgt cattgcttgt tttattagtt aagttaaagt tcgtcctgtt atccgaactt
     4801 gtttttgaga aggagacact tgcatgacgt gtatcgcttg tggtagacga cgtgtctgta
     4861 ctatcgcttg aagttgatga atctgagcta tcacttgtgg tagaggatgt atctgtactg
     4921 tcacttgaag ctgacgtgtc cgtgctatca cttgtagtgg tagatgaatc tgagctatca
     4981 cttgtggtag aggtagtatc tgtactgtca cttgaagctg acgtgtccgt gctatcactt
     5041 gttgtggtag atgtatccgt actgtcgctt gaagctgacg tgtccgtgct
//
LOCUS       DAYYME010000158         5059 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000158 DAYYME010000000
VERSION     DAYYME010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.159"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            42..1183
                     /locus_tag="ACHBFW_002558"
                     /pseudo
     CDS             42..1183
                     /locus_tag="ACHBFW_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1309..2388)
                     /locus_tag="ACHBFW_002559"
     CDS             complement(1309..2388)
                     /locus_tag="ACHBFW_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9318822.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2491..2931)
                     /locus_tag="ACHBFW_002560"
     CDS             complement(2491..2931)
                     /locus_tag="ACHBFW_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9318823.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2988..3398)
                     /locus_tag="ACHBFW_002561"
     CDS             complement(2988..3398)
                     /locus_tag="ACHBFW_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM9318824.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3578..4012)
                     /locus_tag="ACHBFW_002562"
     CDS             complement(3578..4012)
                     /locus_tag="ACHBFW_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9318825.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4112..4960)
                     /locus_tag="ACHBFW_002563"
     CDS             complement(4112..4960)
                     /locus_tag="ACHBFW_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9318826.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         1842 a          972 c          846 g         1399 t
ORIGIN      
        1 cttatttctt gcatacaaaa aaatcaagga gtgagagagt tatgagatat tcaaccatct
       61 taatatcaac atttgcagcc ttaagcttat cgcttactat aaatcagaca gtccacacag
      121 aggagacaga aacagccaaa tggacagaaa ccaagtgggc caacgtcaat cgaattacct
      181 ttcaggatga attttcatcc ttcaattcag gagtcgcaaa attaagaaaa ggacaaacag
      241 cgaccatcaa agcaaaaatt gactattcgg gagacttagc acctatacta caatcagctg
      301 ttacatttga gaatgttgat cccactttgt cgattggcta tgacgagaac actttgacta
      361 tccaacacaa gcaagcgata tttaccttca ccgttacatt aaatcaggac ttgttaaaac
      421 cagcgctttt tactgtcaag gtatcagatg gactaccgaa cgatcatcat ttaacgccgt
      481 atagtcagcg gcagacaatt gagcaagatt cagccatcga ctcaggagga gatcttgttg
      541 aagaaccaac agataaacca gaaaatgaaa ataagccaga agtaccgcct acagaaaatc
      601 cagacggaga acagaaaccg gaaatagaac ctggagaaga acccgataca gaaactcaac
      661 cagagccaga caataaatca aaacctgaaa taacccctgg agaaaaacca gatgtaactc
      721 cagaaccaga tacggattct ggaaaccaaa cagtaccgga aacaaatcca gacacagata
      781 atgaaactga aaatccagaa aaaccagagg tagatccaga agaaaaacca gatgtgactc
      841 cagaaccaga tacggacgca agagaccaag gaataccaga aaaaataaac aaaaaaacaa
      901 ttcaagagga tggaaaaaaa gagagtaaaa aatcaaatct agcaattcta aaaataaatg
      961 aagagcagct gaataaaaaa tcacgaatat ttgatagcgc ccaatcagct gaaaccttaa
     1021 aaagtagcaa ggatacaacc tttgctgtcc tgaaacgaaa aataaacagc taccaaaatc
     1081 cggagagtca caaaacaaag ttatactgtg gagcggaatc attctattaa gtatagcaac
     1141 aatgctttct gcaaaaagat ttaaacaaaa cagatctctc taacatttga ataatgtctg
     1201 tgaacgcatt tttcaaataa aaagaaatat ccagaaaatg accccaaaat accagattga
     1261 aatctgatat ttggggtcgt tcatacttta tctttcttca tatttatttt aagggatatc
     1321 tgtttctttt ggaagatggt tcgggatctt tttcactttc aatacatcta aaaagaaggt
     1381 aaagatcgga atgccgacaa tcaagcccca aacgccaaag aaccgttcgc taacaagtaa
     1441 gaccacaaat gtataaaaaa tcggcagttc tgttttgctg gacatcaact tcggattcag
     1501 cacataagac tcaattagat ggacaatcaa aatcgtaatc aggatataaa caacatctcg
     1561 gatgcctcct tgggaataag ctatgaaaga tagaggaata caagaaatga taacacctgc
     1621 tactgggatc agacttaaaa tgaaaatcat gatcgctaaa ctcagcagct gatgaaagcc
     1681 aaatacagcc aaggcgattg tagtgagcaa cgtatttaaa agagcgacaa caaattgagc
     1741 ttctaatacc aaaccaaatg tattgacaaa cttatcggca aaatagtaaa tatcttgaaa
     1801 gaaccaagaa aattcacttt ttaaaaacaa tcttgagaat acaattgttt ttttcttttc
     1861 aatcataaaa aagaaactta agatgaatga catagcaaac gccactgcca tttttcctat
     1921 atcctgtaaa tagccaagcg caatcgaagc gccattttga agctgttcaa aaaaatcgtt
     1981 tcgctcaata taagtatgga tatattgtaa aacaggatct gcatcttttg gctgatgctc
     2041 gtaaaaatta ataacggact ggatcatgtc aaatgtttga tgaataagca tcgggatata
     2101 ctttgtgaat gccagataga taaggtaaac gatcagtgta taaatcatga tagctaaaac
     2161 acctgtaggg attcggacaa atcgctgaat gatttgaaca agatgaatca tcaaataagt
     2221 aaagataaat gtgagcaaaa tggttgtaat catacttcta actaagaaaa gaacaaaaac
     2281 gacaaataat aaaacagtga gacggcgtat tttttcattc gccaaaaaac gctgataaac
     2341 ggtcaaatcg acaacctcca aataacaaaa aataaacacg ttattcatta tatgatggaa
     2401 tatgaaaata agaaagagca aaaagaaaaa ccgctaaatg acgttatctt atgagttggt
     2461 tttcctcata aaatcgttca tctagcagtt tcagcccttt ttctggtctt gatgaaaatt
     2521 atctagcatt tttcgggtca gatgttgcag tgtatccaat tcttcctgtt caagtggaag
     2581 ataagacttg atcttttgtg gaataccttc tgcttgtttt tgcaacgcag caccttgagc
     2641 agtcagcgta acatttacga ttctttcatc acccgtttca cgctgacgat taatataatt
     2701 tttttctgct aactttttta gtaagggagt cagcgtacca gaatccagac acaaatcatt
     2761 tcctagctct ttcactgaag tctttttctt ttcccataaa actaacatca caagatactg
     2821 ggtataggta agattcagcg gcttgaggag gggagtgtag tgtctaatga tttctttaga
     2881 ggcggcatat aaaggaaaac atacttgatt aactaaatat agatcttcca tttttccacc
     2941 tccgctgttt tactatagtg tatcatagcg gatttacaca aaaaagtcta ttcactaact
     3001 gctttcagag taacttctat atttccagct gtagcttttg aatacggaca cactttatgc
     3061 gctgcctcta ataattcttg cgtttcttcc agagaaagac cttcgataga tccttcaatc
     3121 tctacaccta agacaaagct cgtttcactt tctgaataaa gggaaacttg tgcactgaca
     3181 gtagaagccc cagagatatt tttttgctgt ttgatcaatt ctaatgcact gttaaaacat
     3241 gcactgaaac cagctgcaaa caattgttca gggttggttg cattttcgac gcgtttacca
     3301 ggagctgcaa tattcaattc gaatgtacga tcgggattat gcacttctcc agagcgtccg
     3361 cctgtgttga tcattttcgt agaatacatt tttttcataa agttaccacc tttaattata
     3421 ttttatacaa ttgaatttta cacaatataa ttgtggaaag caaagaaatg gattggcata
     3481 agataaataa gagaaagcac atgaaacaga cgcgctttct cgaatagcaa acatatacaa
     3541 aaaatatcag agacaaaaaa taatctcatc ataaacatta catatatttc tcaaaaagtt
     3601 gttttctttc ttcaaaaatt acttcacgat ggacctgatg aagcatccat aaataaccaa
     3661 aaggatctct gaacattgag ttagtcacac caaagtccat catttctgtc atttcttgaa
     3721 tctctacaca gccatttcgt aatgctttat tatgtgtgtc ctcgatatca gagacgatga
     3781 tattgaacca gatcgtattt ggatgttcag cagtaggagc agtcaaacca agagacgtgt
     3841 tttcatccag catatggaat ctcgtaccat acaaggtaaa aatgacttca ttattgcctc
     3901 tagggtaatc tgttgcctca atcagttgta tttcatcaaa gattgtttga tacagttcta
     3961 gtgctttcaa actatcattt aatacaaaat cgatttccgt tcctttatgc ataaagatct
     4021 cctctccttt tcagtcgaac agttactaga ttcttttatt ataccagcta aagaaaacgc
     4081 attcaaaaaa atagcggatt tacttaccaa ctcattcgat tggatgataa gtaaatccgc
     4141 gtcccattac ttctgctacg ctgctgacaa tgacaaaggc acttggatcg atttcttcta
     4201 tcaatggttt cattcgggaa aattcatgat cagtcacaac gatcaataaa acctcttttg
     4261 actttcgatt atacccgcct tttgcctcta gcagggtaag gccacgtcca atctcagcac
     4321 ttaagcgaag gcggatgtct tccaaataat gttcactcat gaccataaca gatttctttc
     4381 gattgatacc tgtttcgata taagtcatga caattgaagt gattgcaata gataaaatag
     4441 cataaagaaa ctcttctatt ccaaaaataa acagattaag agaaacaacg aatgcgtctg
     4501 taaaaaataa gccaatcgaa gtattcaagt ggaaatattt tttaaaaatg aggggaggga
     4561 tcgtcgtacc gccgctagaa gagttgtttt tatataaaat cgcaactcct aaagcaaaaa
     4621 tcgcacttcc aaaaatgaca gacaacaacc gatctgaagt aatcattgtt tctggaacta
     4681 ttgcaatgat taaaggaaaa accaagcttc cgaaaagtac tttaaaaaag ggttttttac
     4741 ctaaaaatag aagagctagt accaacataa cgatattcaa cactaaaata actatagctc
     4801 ggtcaaagcc taaagcagct tccagtaata ttcctagacc gctgacacct ccagcagcaa
     4861 tatgatgagg ccctaaaaac atattgatag cgactcctaa tatgaagagg gcaaaaataa
     4921 tcttaatgta tgtccatata aattgtctcg tttctttcat ttattcactt ccttaattca
     4981 ttaataatac tcttatctta ttataactta cttttcacct taagttaact ttaattaata
     5041 aaaaaattag gctagtcta
//
LOCUS       DAYYME010000159         5013 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000159 DAYYME010000000
VERSION     DAYYME010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.160"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..437)
                     /locus_tag="ACHBFW_002564"
     CDS             complement(<1..437)
                     /locus_tag="ACHBFW_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318827.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFC"
     gene            656..1876
                     /gene="sstT"
                     /locus_tag="ACHBFW_002565"
     CDS             656..1876
                     /gene="sstT"
                     /locus_tag="ACHBFW_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFM9318828.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            2023..2658
                     /locus_tag="ACHBFW_002566"
     CDS             2023..2658
                     /locus_tag="ACHBFW_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM9318829.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            2849..3589
                     /gene="truA"
                     /locus_tag="ACHBFW_002567"
     CDS             2849..3589
                     /gene="truA"
                     /locus_tag="ACHBFW_002567"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM9318830.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3734..>5013)
                     /locus_tag="ACHBFW_002568"
     CDS             complement(3734..>5013)
                     /locus_tag="ACHBFW_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318831.1"
                     /translation="RYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCY
                     EIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLK
                     ATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLF
                     LFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNG
                     FSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAP
                     EDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSN
                     NKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIA
                     SRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         1638 a          762 c          984 g         1629 t
ORIGIN      
        1 ctacaaaaaa gcaaagtaca agaaacagca taagtaaagt aattctgact gataggtgtt
       61 aggataagcc aataatcacg aatattcaag ctgccagctt gtacgtaaaa aaagatattg
      121 caaactagtg tggctattag gaatataaag gcaaaacgac ttaaataatg taaaactttt
      181 aaacgggcga ttttttgata ttggtaaccc atgtaaagtg ggatcaaatt tgcaccaata
      241 acaacggaag tttgtaaaaa tgtatagatg tagaatcgac cagggtttgt tgcatatgta
      301 ggaatagtga ctgctgaaat caatatacgc tgtaaaacca tcattataca agcgacaaag
      361 attgtcagga agccataact ctccttcttt atttttttca agaataaaca cctcacaaaa
      421 aaattggaca aaaacataag aaaacgtttc ctgataagtt gcccattaat aacatcctaa
      481 cttagaatat ctcaaaaaac aacgtgtaga cagaaaaagt acaaaattag caagaaatgg
      541 ttgtttgtat tgttagaatt tttagaaaat tataccttta gtttctttgc ataaaatgat
      601 acaatgattg ttaaaattgt gaaagggtgg tcatttgtta gagaagtttt tggatatgtc
      661 gttagttcaa cgtattgttg ttggtatttt agtcggaata ttgtttggta tctttttccc
      721 cacatggaca tttatttcaa tattgggaac gttgtttgtc agttgcttga aggcaatcgc
      781 acctattctg gtttttttct tgacgatggc gtcaattgcc aagcataaaa ttgggaataa
      841 gacattcgtg aagcctattt tgatcttata tttagtggga acattgcttt ctgcacttgt
      901 agcagttatt gtaagttcaa tttttactgt tcctattact ttacaagaaa cagtatctga
      961 aaaagcaccg caaagtttgg aaagtgtttt atctactatg ttgacgaatg tcacacaaaa
     1021 tcctgttcaa tctcttattg atgcgaatta ccttggcgtc ttattctggg ctgtattgtt
     1081 gggactagct tttcgagcac gttcagaatc gacaaaagag ttggtggatc agatatctat
     1141 agcgttgtca caagtcgtac aagtgattat tgcatttgct ccgataggga ttttaggatt
     1201 ggtttatcaa tcaattgcta cgactggaat cgctggattg gcagagtatc tgcagctttt
     1261 acttgttttg atcggtacga tgctgtttgt cgcattagtt gtgtacccat tcctcacgtt
     1321 tttgtttatt aaagaaaatc cctatccatt gattttcttt tgtttgaaag aaagcgcact
     1381 tcccgctttc tttacccgaa gttctgccgc aaatatcccg ataaacatga tgttggctga
     1441 aagattgaaa ttgacaaaag agtcttattc catatcgatc ccgttaggtg ctacgatcaa
     1501 tatgggcggt gccgcgatta ccatttcctt gatgacgttg actgctgtac attctttagg
     1561 gattgaagca ccttttgttt tgaaagtcct tttatgtatt ttgtctgctt tagctgcttg
     1621 tggagcttca ggtgttgcgg gcggttcact gttattgatt ccattagcat gtagtttgtt
     1681 tggtatctct aatgatgtcg cgatgcaaat cgtcggtatt ggatttatta tcggtgttat
     1741 ccaggattca atggaaactg ccttaaattc gtcaagtgat ctgttgttta ctgcaatagg
     1801 agagctaggc gcgcgtaaaa gaagtgggga aaaaatcaaa ttgaaagatt gtttagatgg
     1861 attaagtgaa cgttgaacgt tagatattta gtatttttaa aatgattcga gtttttaatg
     1921 agttatatag atgttcgtgt aaatagaggt ttctgttaaa tcaagataaa gtcgtttata
     1981 ataacaatta aaagcatagg taattgttat tggaggcgta tcatggattt tttacgtaaa
     2041 tttggttttt tctttttatt attaggcgtg ctgagtgatt ttttgactcc gtacgtatta
     2101 ggattgtatt atcctgaatt agatcaaatg aagagcgtga tcagtgtatt tggagaggtt
     2161 tccagtcctg taagaaaagc gtttcttatt tggtctgtgg tttcagggat ctttttcatc
     2221 ctgtcattgc ctgcattgta tgctgcattt gttagtacat ctcgtgtgtt agctacccta
     2281 gttacagcag ccatcgcgtt ctatggtata ggtgattgta tctttacagg tttgttcagt
     2341 atcaataccc atgaatcttc gtggaatgtc tcgacatggg tccataatat cggatcaggt
     2401 ttaggttaca gtggtttttt attgtttcca gttcttcttt tcttgatcta tcgaaaaaat
     2461 gggcaaatgg acgtaagcca cttatacctt ggactgtctg tgatcagttt gctttttgcg
     2521 gcgatttatg gactggcaag aattccttct ataaattctt ggccattact gaataaaata
     2581 ggtttttgcc aaagaatcag ttttttcttt aattatgtgc ccattgcctg gttgagtatc
     2641 ttgcatctca aacagtaaaa cttgagccgt caaattgaac agtcagcgga tacttcatcc
     2701 ggtggctgtt ttttgttttt ctgaaatata tatttccaca gttttcttgt atagcttctg
     2761 tcttagagaa gcaaggagct atattttgtg ctacaatctt aaaaaggtag agagaagacc
     2821 aaatggaaga cagaggagcc actaaaatat gagaaacatc aagttgacaa tcgaatatga
     2881 cggaaaaaga tattcaggat ggcaaagact tggagatgac gataaaacca ttcaaggaaa
     2941 aatcgaaaaa gtattgcatc agatgacaaa tgaagagata gaaattattg gatcaggaag
     3001 aacagatgct ggcacccatg cgcgaggaca agtagcaaat tttaaaacaa acacagaaat
     3061 tggactggca gaaatgatcg actttatgaa tcgttacctt ccgcgggata ttgtcatcaa
     3121 aagaatcgaa gaaatgcctg agcgcttcca ttcaagatat aatgcggtgg gcaaaaaata
     3181 cagctattat gtttggaaca acgtgattcc ttcagcattt gagcgaaacc acagctttta
     3241 tttccctcaa gagttggata tggacaagct gaatgcagct tgcgaaaaat tagtgggtac
     3301 acatgatttc attggttttt ctgcattgaa aaaatcaaaa aaatccacag tgcgtacgat
     3361 tgatgaatta tctatcgaac gaaagggaga aatgcttcat tttacttttg tcggagacgg
     3421 attcttatat aaaatggtgc ggattatcat ggggacatta ctagaaatcg gcacaggtgc
     3481 catgccgata gaaacaatcg atgaaatatt tgaaagcaaa gtgcggcaac atgcaggaga
     3541 aaccgtccct gcacaaggtc tgtttttgga tgaagtttat tatcgataaa aatactttga
     3601 taatggatga aacgcatgtt tcacagtata aatatcgatg ttaaataatg tatgaagcaa
     3661 attaaagaaa tataaaaaaa gactttttag cctgtttatg tgtacagcta aaaagtcttt
     3721 cttaaataaa aatctagtga gaatgttgta cttctcttct cggttgtttt tgtttagaaa
     3781 aagtaaatcg ataaagtaaa aataaaatca aagtaacgat agataaatat cttgaagcaa
     3841 ttgctagttt tgtgtactga taagtaataa cgactttatt tatacctgga ggaatagtta
     3901 attcagttgt gccaaactta gataattttg tttgtactag tttgccatta atagaagcca
     3961 cttgcccttt ataacgataa ataggtgtcg tcaagacagt atttttatta ttcgaattat
     4021 ctaattcaat tgtaagtttg ttactcataa aatgagttgt tggatttatt tcctggttac
     4081 ctagtaaata ttgatcatta ttaatcatat caggatgata gattgactct ttgtttgcat
     4141 aatcagtatg attgtaattt tcagctatag caacagcgtc ttcaggtgct ataagtgtta
     4201 atgaatcttt gttagcatgg atagaattgc ttactcctga gagatgcaat attagtgcaa
     4261 gaaacgtaaa gaccattatt ttccatgaat tatttttagt agaaaaaata taatagatca
     4321 ctatactcat cgtataggct ataaataaag aagaaaaacc atttaaccgc cagacaaatt
     4381 gtagactctt aactggtgta tggttaaaaa gttgccaagg aaataaattg gttgaacaaa
     4441 aaagaacaaa tacaccaaga aagaaaataa ataaatcatc agcagttaat ttttttataa
     4501 aaataaaagc accaatcaat ccgagaaata agaataatcc aatagtatag ttgttatagt
     4561 tattatttaa aatatgtgtc agcatatcac taggagccat accacttaat tcttttccca
     4621 gtgggacttt caattcttgt gctctagatt gttgaataaa aggaattaag aatcccgcac
     4681 ttaaaaaaaa ggtgaggacg gtagctttta aaagacttaa gagtctagcg attttttggt
     4741 cccaaaaata aaaagaaaga aacaacgttc ctccaataaa tacagaaacc atagcgacag
     4801 aaagtaaatg agtatacact actagcgtca tgccaataga caaccagtac catttttggt
     4861 aatctctaat ataaatttcg taacacccca ttagtatcaa aggcaaaaat gttaaagcga
     4921 cagcttctcc caatgaagca cgatggaata tgtcaatggc acgataggcg gaaaatgtat
     4981 aaataatcga aaataacagc gaaatataac gat
//
LOCUS       DAYYME010000160         5000 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000160 DAYYME010000000
VERSION     DAYYME010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.161"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            69..1289
                     /locus_tag="ACHBFW_002569"
     CDS             69..1289
                     /locus_tag="ACHBFW_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM9318832.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1286..4006
                     /locus_tag="ACHBFW_002570"
     CDS             1286..4006
                     /locus_tag="ACHBFW_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9318833.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            3990..4934
                     /locus_tag="ACHBFW_002571"
     CDS             3990..4934
                     /locus_tag="ACHBFW_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM9318834.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         1857 a          819 c         1005 g         1319 t
ORIGIN      
        1 ttttcggtta ttgatgaaaa aaaacattga aaatggtatg ataaaagaaa aaaaggagga
       61 aatttttaat ggtgaaattt atccatgcgg cagatttaca tatggatcgg tcatttgaag
      121 gactgacaac tcttgataaa actgtacagg aaaaactatt gaagacgaat ctgaccgttt
      181 tatcaaatat cattgaacag gcaataataa ataacgttga ttttgtttta cttgcaggag
      241 ataattttca tcaaaatcgt ccttcattaa aaatacagaa acatttttca gaacagatga
      301 aacggttaga aaagaaccat atacctgtat ttataatatt cggaaatcat gatttctatc
      361 aaaaagagcg gtattggttc tcctttccta aaaatgtcca tttatttaca tccgaaacgg
      421 tagagacaaa aaaaataacg ataaaaagtg gagaaactgt ctctctcagt ggatttagtt
      481 atcgtcaacc gtggattcaa aaagacaaag tgatggaatt tccttctaga gaactcacag
      541 attaccatat tgggttgtac cacggggaac ctggcgtctc tcaaaaagga aactatgctc
      601 ctttccaacc ctcaaagatg caagaaaagg gatacgatta ttgggcttta gggcatatcc
      661 atgtgccaac ggtcttaaat gaaaagcaaa caattgttta tccaggtgcg cctcaaggtc
      721 atactaaaaa ggaacaagct tctacatcta ttctactagt tgaattaagt aaaggaagtt
      781 gccaaataca tccgattaaa gtagcagagg tctattggaa gacaaaggaa atatcattga
      841 ggaaagcaag aacgacaaaa gaggtcattg ttcacattcg tcagcagctt tcaaaagtag
      901 aggatggatt tagtttaatt gagataaaat taaaagatta tgaccatctc aatacagatg
      961 ttttggaacg gatacaatca ggagagctat tagactattt gttagaggaa ttctcagata
     1021 ggataaatga tttctttatt tggcgtattt ctctcataga gaatactttt tatacgaaga
     1081 ccccacttgc tgcttctgct aaattgatgg aacagttatt tcaatactat cagacacctc
     1141 aagatttcca acaaatttta aatgaagtat atagtcatca agaagcggct agaatattga
     1201 gcgaactgcc ggaatatcaa gaagaaacac ttgaaaaagc caagcagctg cttaatcaag
     1261 attttttatt tgaggaggat caggaatgaa gattttaagg atcgagattg ctgcttttgg
     1321 aaaatggcgt caaaaaagtt ttgactttta ttcaggcaat cagctgatct atgggggaaa
     1381 cgaagcagga aaatcaacga tttatcaatt tattcaagca atattatttg gtttcccttc
     1441 aaaaggccgt aaaaaaaagg attatacacc aaaagacggc tcagcctatg gcggcaaaat
     1501 atggcttaaa catcctgtat atggtgaatt tgctgtcgag cgctacaagc agcaaaatcg
     1561 cggaaaatcg aaggtgtggc tgggggatca agtgggaagt gatgagttac tagaaaaact
     1621 gatcgcacct ttgacgaaag agttattcca gcaggtattt actttccagc aagagcagtt
     1681 gatggaacta gatcatttga gagaaaaaga gctacatgat gcgctgattt ctttaggcat
     1741 cacgggcagt tctcaagtat tccaaaagcg gcaagaatat tatcagaaag cacaacaatt
     1801 attcaagaaa aaaggccaaa aattactaat caatcaaaaa ttaaatgaat ggaaagagct
     1861 gcaagaaaaa attcatcaaa aacaagaaca ggaagcttat tttggcaagt tgatccaaca
     1921 agaacaggaa tacgagtcgc aacagcagca gttattggca caattaaaag aggcgaacaa
     1981 acaattttca ttagccagac agcaagaact taatttttct ttatatgaag aatggcaatc
     2041 tctcaaaaaa aacagcacta caggtcttcc tgatgagcaa cttcttttag atctggagtc
     2101 tttctccaaa cggtatcagc aaacaaatga gcgtttggaa gaattagaag caacactgga
     2161 aaagcatagc gggatggatc agcagtcagc cagatattat ttttatttag agcaggagca
     2221 ggaactaaaa gatctccagc aacttcaatt tacagcggaa caattaaacg aagaatggca
     2281 aagattacaa attgagacta gagaaacaaa ttcagaattg aagattttgg aacaaaggtg
     2341 gcattggcag aaaaatccgt taccggaaga atttttatat gaagaagaat ggaagcatct
     2401 tctggctgaa gaaagaagga ttgctgatct agaaagccag atttctgtgc gtctccaaat
     2461 agctaaagaa cagcaagaga tgattgaagc agaagtgaat gacttggaag agacctatcc
     2521 agcattatta caaaatcagt cagagatagc accagtctct tcatctaaac cgtatatgct
     2581 gatggctggc ggagcaataa tcattgcagg tatatttgta ccaatgccct tgaaagtgat
     2641 ccttttacta attggtgcag gcatgattgg ctatttcttc tacaaaaggc aaccaaaaat
     2701 taaaactaaa aatgatttct ctgagataaa aggaatgtgg caagaaaaac taggacaact
     2761 tgatctagcc ttagctcaat tagctgaagc agaagaaaaa caaaagagaa ctggagaaga
     2821 aaaacagatt cttcagcaag aagtatatcg ttttgcggat catcggcact taggaaagat
     2881 ggatacgctc aaactcatga aagaatatgg acaggaagtc atagattatc agaaattata
     2941 tggcgtattt gagcggaaac ataaaagaga acaggaaata caagaaaaac tacgacaaat
     3001 cgaccaaaaa tttgagtttg ttaaagaatg gttaccgata caagataaga caatttcaga
     3061 aaaaatggta ttgctttccc gttttataca agagatggaa cagatgaaat ttgccagaag
     3121 ctatcagcaa aatactttat tgaagcaaga aatcaatcaa ttgaaaaaga aacaaaaagt
     3181 atggatacaa gaatacagcg aacaattgac tcaagcaggc ttatcttatc cttcggaagt
     3241 acctgtcttt ttacaaaaag ccaaacaagc cgaacagcag atgaaaagat acaatgaatt
     3301 atcggaaatg cttactccac tatttcctga agagctgaca aaagagaaat tatctgtaag
     3361 actgcgccaa ctgaaagaaa agcaatccca actccagcaa gaagaaaaag aagcaagtga
     3421 aaatcggcaa cgactccaac ttcaagtaga agagttgcaa agagacggca cattggacga
     3481 actttatcaa caagctagcc ggcaaaaagc aaaattacag gaattactgg aggaatgggg
     3541 agcacttgaa atcgccggga cattcttagg tgatctttca acagagatga gtgaaagaca
     3601 attaccccag ttgttaagag cagcaggtga ttatttaggt attttaacaa aaggaaatta
     3661 tcagaagata ttactgaaaa atgatacact gactttaaca gacggatata cagaatttcc
     3721 catctatgag ttatctacag gtacgaaaga tcagctgatt atggcgatga gatttgctta
     3781 cctctcatta gaagctaacc gttcgatatg cccgatcatc attgatgatg gctggcttca
     3841 ttatgatcat cagcgaaagt atcaattagc tgaactttta gctaaatttg gagaaaagta
     3901 tcaaataatt tgtttttctt ctgataaaga aatggtaagc tattaccatg agatgaacca
     3961 aatcgtaaaa caattagaag gagcaaataa tgaaaaaaat tcgtgattta gtaatcgatg
     4021 agcagtttga acaatttgtc ctgatcaaaa gtgcagatgt aagaatcgct aaaaacggaa
     4081 aaaaatttat tgcttttact ttccaagata cttcaggtac tatcgatggt aaatactggg
     4141 atgcttcgga ggaggaaatc aataaattta cagccggcac agtcgttttt ctaaacggta
     4201 aaagagaggt ttatcaagga aaccctcaag taaagattct tcatatgcgc ttaacaaggg
     4261 tagatgagcc aggagagcct agtttataca tggaaagagc ccctttgaag aaagaagaaa
     4321 tggaagaaga aatcaatcaa ttgttgtttg agatcaccaa tgcgcattgg aatcgtattg
     4381 ttcgctatct acttaataaa tatcaaaaac aattttttga gtttccggca gcaaaaagaa
     4441 accatcatgc atttgccagt ggattagctt atcatacttt gacaatgctt cacttaggga
     4501 aatcaatcgc cgatgaatat cctgagctga atcgagcatt gttgtatgct ggtatcattt
     4561 tgcatgattt aggaaaggtg attgaacttt ccggtcctat gtcgacagaa tatacgcttg
     4621 caggaaattt gatcggtcat ctggtattga ttgacgaaga aataactaaa gcctgtcttg
     4681 cgttgaaaat cagcgaagct gacgaagatg ttctcgtatt acgtcatatg gttttagcac
     4741 accatggctt attagaatat ggctcacctg tacgtccaag aataatggaa gcagaaatat
     4801 tacatcaaat cgacaatatc gatgcttcga tgcagatgat gttgacttcg attcgtcaaa
     4861 cagaaccagg aaaatacacc gatcggattt tcggtatgga caaccgaagt ttctatgtac
     4921 caaaagattt atagcaaaaa tttatttttt tcattcaaaa caaaaaaagc gagacataag
     4981 tcttcgcttt ttttgttttc
//
LOCUS       DAYYME010000161         4996 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000161 DAYYME010000000
VERSION     DAYYME010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.162"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..571
                     /locus_tag="ACHBFW_002572"
     CDS             <1..571
                     /locus_tag="ACHBFW_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9318835.1"
                     /translation="NDAQGIVLMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFF
                     SAIVYLILFLIKASNMYVFLILSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDG
                     TVYAVYSFARKIGQALAGGVGGWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFL
                     IVGLILFFLYPLSKDKVLENCELLKQKHK"
     gene            581..1375
                     /locus_tag="ACHBFW_002573"
     CDS             581..1375
                     /locus_tag="ACHBFW_002573"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9318836.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            1388..3154
                     /locus_tag="ACHBFW_002574"
     CDS             1388..3154
                     /locus_tag="ACHBFW_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFM9318837.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            3177..4985
                     /gene="uidA"
                     /locus_tag="ACHBFW_002575"
     CDS             3177..4985
                     /gene="uidA"
                     /locus_tag="ACHBFW_002575"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFM9318838.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
BASE COUNT         1804 a          597 c         1003 g         1592 t
ORIGIN      
        1 taatgatgca cagggaatag ttttaatgaa ttttatcaat cctattttgg ttttaatagt
       61 tgtttcgcct ttaactttgt ttttaagtaa aaaatatgga aaaaaagaac tggcatcagt
      121 aggaatgttc ttttcagcaa tagtatattt gatcttattt ttgataaaag ctagtaatat
      181 gtatgtattc ttaattctta gttcggttgg ctatatgggt ttgggtgtgt ttaatactgt
      241 tatttgggca aatattactg atgttattga tgatcaagaa gtaaagtctg atcaaagaga
      301 agatgggacc gtatatgcgg tatattcttt tgctagaaaa ataggtcaag ctctagctgg
      361 aggcgtcgga ggttggactc tttctattat aggctatgac tctttagcaa atgtacaaac
      421 gggagatgtt ttagaaaagt tatataacgc ttcaacatta attccatcaa tttgttttct
      481 gattgtagga ttaattttat ttttccttta tccattatct aaagataaag tcttagaaaa
      541 ttgcgagctt ttaaaacaaa aacataaata ggagagaaat atgaataatt ggatattttg
      601 tattgattca gatggctgtg ttatggatac gatgacttat aaacacaatc tctttttcgg
      661 accaattgct gcagagattt ttgatgttca tcatagagaa aaattcctac aggattggaa
      721 taatattaat ttatattcag agactcgtgg aataaacaga tttttaggtt tagttaaaac
      781 attagaaaaa aatgatgtaa catcgattaa tcgattaaag cactgggtat ctctaacaaa
      841 agaattgtct aatccatctt tgaagaggga aatagaatat tctcagtcag aggatttatt
      901 aaaagcttta aaatggtcgg aagaagtcaa tcaacagatt agtcaagcaa aaggattaga
      961 taaacccttt ttaagtgcta gagatactat aaaagctctg aaaaaatatg ggaagataat
     1021 tattgtaagc tcagctaata aggaagctgt tcaagaagaa tgggaaagac atgaattatt
     1081 atcattagtt gatgaactgt gttgtcaaga taaaggaaaa aaagaagata ttattcgaag
     1141 tgtaattgaa aatggttgtg atttggataa gatattaatg attggtgatt ctcctggtga
     1201 tctagaagct gcaaataaaa ataaagtttt cttttatcct attttggtca ataaagaaaa
     1261 agaatcatgg gaaaacttaa gaactgatgt tttatttaaa tgtttatcgg gagattatgt
     1321 agatattgaa caaaaatata tagatacttt ttggaaaaat cttactaata agtagaggag
     1381 aaagaaaatg actataaaag tagatttacg aaaaaaacca tataatttag atgaagatgc
     1441 tatttcttgg attgaagaga cgattgattc tatgactttg gaagaaaaaa taggtcaatt
     1501 gtttgttaat atgggatcta gtcgtgataa aaattacttg agtaatatgg tcaatcagta
     1561 ccatattgga gctgtacgat ataatccagg aactgctgat gaggtatatg atcaaaacta
     1621 tatcttacaa acagaaagta aaattcctct attaattgct gctaatacgg aagcaggtgg
     1681 taacggagct tgtacagatg gtactgaagt tggtgtagaa gtaaaaattg gtgcaacaaa
     1741 tgatgcgaaa tatgcttatg aattagggag agtttctggg attgaagcat cggctattgg
     1801 atgtaactgg agttttgctc ctattgtaga tattaataga aattggagaa atcctatcat
     1861 ttctagtcga tcttttggac aaaaccctga taaagtttta gaacttagtt tagcttatat
     1921 gaaaggtatt caggagagtg gaattttgcc tgcagctaaa catttccctg gtgacggtat
     1981 tgacgaacga gatcagcatc tatcttttag tattaatagt ttaggtacgg aagaatggga
     2041 taattctttt ggaaaagtat actcaggctt aatagaagct ggattacctt caattatggc
     2101 aggtcatatc catatgccag cttattcaaa acattttaat cctgatctga aagatgaaga
     2161 tttacttcct gcaactttat caaaagaatt aatcactgac ttacttagag gaaaactcgg
     2221 atttaatgga ttagtagtta ctgatgctag tcatatgtta ggaatgactg gggcaatgaa
     2281 acgatcagaa ttattgccca cttctattgc agctggatgt gatctatttc tattttttaa
     2341 tgatccagat gaagacttta attatatgat ggaaggctat cgtaaaggaa ttatcactga
     2401 tgatcggtta ttagatgcct taaaaagaat tttagggtta aaagctaaat tgggattaca
     2461 caaaaaagca aaagaacaaa ttttattacc taagcaagaa gctctggaaa aaattggttt
     2521 aattgaaaat aaatcaattt ttaaagaggt ggctgaccaa gcaattacat tagttaaaga
     2581 taaacaagat atttggccta ttgtaccaga aaaatataaa aggattttac tagtagatgt
     2641 acatggggta aaaggtgggt ttggtgaagt agttgctgga aatagacctc aagccattga
     2701 tttattgaaa cagattttgt cagcaaaagg atttgaagtt gaaaaataca tttcacctga
     2761 agaagaattg aaaggattat cgcaagaaga aaaaattaaa aatatagctg gaatatatgc
     2821 tgctaagaga ccaattaaaa atttaactaa aaaatatgac ttgatcatta atatagctaa
     2881 tgttaatcca ggcacagttc aacgaattgt ctggccagca agtaaaggaa ctccggatat
     2941 accgttttac attcatgaaa ttccaacaat ttttgtatct gtacagtgtc cgtttcattt
     3001 ggctgatgta cctcaagtaa agacttatat taatacttat gatggaaaac aagaaacttt
     3061 agaaatattg gtggacaaac taatgggtct ttctgaattt aagggagtaa gtccaataga
     3121 tgcttattgt ggtttaaagg atacatttat ttaatattta gataggagtg gtttatatgt
     3181 tgtatcccat tatgacagag acgaggcaag taattgactt aagcggtctc tggagattta
     3241 aattagatac aaatgatgac ttacaggaag aacttgcttt agaaccaata aatgaaaaag
     3301 agagttatcc gatttcagtt ccctcttctt ataatgattt atttgaaaca gagaacatta
     3361 gaaatcatgt tggatgggtg tggtatgaaa aagaagtgac tcttcctaaa agattattag
     3421 aagaaagatt agttcttcgt tttggatctg caacacatga agcaaaagtt tttttgaatg
     3481 gaaagtttat tactcaacac aaaggtgggt ttacaccatt tgagtctgaa attaatcggt
     3541 ttgtaaaaga cgggaataat cgaattacag ttgctgtaaa caatattatt gacgatagta
     3601 ctttacctgt ggggattatg aaggaaacag tacaggaaaa tggggatata aaaaaggaaa
     3661 acttagttaa ttttgatttc ttcaattatg caggtattca tagacctgtt aagttatata
     3721 caactccgaa agactatata agtgatatta ctattaatac aagaataact gatttggatg
     3781 caagtgtaga attcaatatc gatacagaat ggaccggaaa aatcttaata gcgatatatg
     3841 acgaagaggc tcaacttgta gctgaagctg aaggaaagaa aggaatgatt cagcttaaga
     3901 atccaatttt ttgggaacct ctagacgctt atttatatag tatgaaaatt agtttaattg
     3961 atgataataa agtgatcgat acctatacag aagaatttgg tattcgcagt gttcaggtta
     4021 ccaatggcaa atttttgatt aataataaac ctttttattt taaaggattt ggtaagcatg
     4081 aggattcgta tgttaatgga cgtggtttga atgaggctgt aaatattaaa gattttaatt
     4141 taatgaaatg gataggtgct aattctttta gaacatcaca ttatccttac tcagaagaaa
     4201 ttatgcgatt agcagatcga gaaggtattg tagttattga tgaagtacct gcagtagggt
     4261 tacatttaaa tttcatggcc actttattag atgataatgt ggaaaaacat aatacgtgga
     4321 aagagatacg aacagaaaat tctcataaaa cggtgatcaa ggaattgata gaacgcgaca
     4381 agaaccaccc aagtgttgta atgtggtcta ttgcaaatga accagattcg gattccgaag
     4441 gtgctaaaga atattttgaa cctttagttg aattaactaa agaacttgat ccgcagaaaa
     4501 gacctgtaac aatcgttact tatctaaaat caacgccaga tgtttgtaag gtaggagata
     4561 ttgtcgatgt tttatgttta aatcgttatt atggttggta tgtttcggga ggggatctgg
     4621 cacatgcaaa atatctgttg aaaaaagagc tagatggatg gatggaaaga tgtcctgata
     4681 cgccaataat gtttacagaa tatggagcag atacagtagc tggatttcat gatacagtac
     4741 caacaatgtt tacagaagag tatcaagtgg cttattacga agcaaatcac gaagtaatag
     4801 atacatgtga aaattttgtt ggtgaacaag tgtggaattt tgcagatttt gcaacaagtc
     4861 aagggatttt aagagtgcaa ggaaataaga aaggaatctt tactagagat agaaaaccta
     4921 aaatgattgc ttactatcta cagaaacgat ggtcatcaat acccaatttc agttataaaa
     4981 aataatttgg attttt
//
LOCUS       DAYYME010000162         4993 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000162 DAYYME010000000
VERSION     DAYYME010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.163"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..426)
                     /locus_tag="ACHBFW_002576"
     CDS             complement(<1..426)
                     /locus_tag="ACHBFW_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9318839.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSL"
     gene            complement(437..1105)
                     /locus_tag="ACHBFW_002577"
     CDS             complement(437..1105)
                     /locus_tag="ACHBFW_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM9318840.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1235..1819)
                     /locus_tag="ACHBFW_002578"
     CDS             complement(1235..1819)
                     /locus_tag="ACHBFW_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9318841.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2150..2383)
                     /locus_tag="ACHBFW_002579"
     CDS             complement(2150..2383)
                     /locus_tag="ACHBFW_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM9318842.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(2398..3348)
                     /locus_tag="ACHBFW_002580"
     CDS             complement(2398..3348)
                     /locus_tag="ACHBFW_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFM9318843.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3345..4187)
                     /locus_tag="ACHBFW_002581"
     CDS             complement(3345..4187)
                     /locus_tag="ACHBFW_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFM9318844.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(4189..4908)
                     /locus_tag="ACHBFW_002582"
     CDS             complement(4189..4908)
                     /locus_tag="ACHBFW_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM9318845.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
BASE COUNT         1701 a          902 c          614 g         1776 t
ORIGIN      
        1 tagcgaaaaa atgtttttat ctaatatatt tgaaagatct aataattcag ctaattcatt
       61 gattctttta tccatatgct cttttgtaaa tccaaagttt tccatcgaaa atactaactc
      121 actcgttgta tttgttgtaa agaattgtcc tctaggatct tgacttacgt agccaatatt
      181 ttttgatata ctttcttgtg ttaaggattg tagcgagtta ttttcaaata aaatttcccc
      241 aaccaatttt ccgtcataaa gtcttggaat aagtccattc attaacttta atagtgtgga
      301 tttcccactc ccagaagaac cagtaatcaa tgtaagactg ccttttttta ttgaaaaatt
      361 aaggtcaaca agcgcttgtt ccttaacatc cttatatgaa aaagaaacat tgttaaattc
      421 aaacaattct ttcacctcaa atcaaaatac ttaagaaaaa aatagtaacg gaaaacagga
      481 tgatatagtc taataagctc atgtttgttt tatagtaaga acttcttttc acaggagatt
      541 ctatcccctt tgttattgca ttagcggtca tttcttcagc aatcatagtt gcacgtataa
      601 gaacgggaac taacagaaat tccaaatatt gcagaggctg aaaaaaagac aagcgaatgt
      661 ttctcatttt taaagattct ttaatcattc ttatttcaga acgataagtc ggaaagaatc
      721 tgatcactac tgtaatactt aacattattt ttttcggtaa atgtattttt tctaaagagg
      781 ataataattc attgctagta tagacactaa ccaacaacca tgagatcatt acgaccggca
      841 acattcgaaa tacaatatat aaggatgtcg caaaaaatat agtaaaaaac tctatcttga
      901 tagcatcaat aattttcatt actaaaaaca gaactgaata aatcaagatg aaataaatac
      961 atgtctttat tttacgaaac atcaatacta aaactgtagc aataatgaaa caccccactt
     1021 gaaatatatt tccctttcct aaaaaaataa tgaccggaaa aaaaatattt aaagtaatca
     1081 aggttaacgg attgattttt gacactcttt cacctccaaa aaataagatc gcactgttat
     1141 cttaagctga taaaaatgca ccgttattca gataacagtg cgatcttatc aaaaagcata
     1201 actgagaaca attctcaatg caagcttttt ttaattaact cagtaattca atccacccag
     1261 gaacagaaaa ttgatacaat cttctggtta atagcatagt cttttctttt gaatagctat
     1321 gcttcaatat atcatagaat gtagcgtaga atgagtaaat gtagaagtgg atttcttctt
     1381 cattaaagac taaattttga ttgatcattc cgatagtctt taacttatct agacaacaga
     1441 ctgtcttttt cgtcacaaat gatgtaattt tttcaacaat atcttgatat ttggttccag
     1501 ctgattggaa taaaatcaaa tcgaatatac gtttattctc aaaaatataa ttaataaatg
     1561 gatctaattc attgaagttc tctctccaac ttttctctat atctatattt tctaagttta
     1621 agaggtattt ggtaaactct tcattataca tattaataag cgcattaata tgcggctgaa
     1681 taatttcttc aaataacgct tccttagaag taaaatgttt ataaaaagca cccgtagtta
     1741 cgcctgcggt caggcacagt tgccttatct tagtttgctg aaatccttct ttttcaaaat
     1801 ggcttagtcc actttctatt aacttttggt gagttataat ataatctctg gtttctatgg
     1861 gtacttcctc ctaataagat gaattaactt tattatctta cacaggactt gaagtttcaa
     1921 tcgttaacgt ttattactga actcattcct tagagctaaa ctaatgagaa agagcagaat
     1981 aactgataca aaaagcaatc ctacaagaat taatagactc atctaaatca ttcacctttt
     2041 ctaaaataat ccgagaaaga tttaaaaaaa ttatatccag ctattaggat aagtcctatc
     2101 ataatcactc caatagccat tttcttatcc tccctaaaaa taaagtcttt tacttaaaac
     2161 cttttttgta atcgtcaata aattttttcc ctttatcagt taattgaaat cctatatcta
     2221 aatagtcctc atatctaagt ttagccagca tctcacttac agatgctgga ctaacactta
     2281 gaacttttgc taaatcatta ttttttaatt tcttttttct ttttgataat tcctgaattg
     2341 ctagtaaata aattagtata gtagtcgttt taatttgtac catacctacc ctccaaatta
     2401 tttcgaaagg ccttcagcaa ttttatccag gttccatttc atcatcgcat agtaactatc
     2461 accatcttgt cctttttctg caattgaatc agtaaaaatc gttgaataga taggaatatt
     2521 ggtatctttt gatactgttt tcattggtct atcatccaca ctgctttcta cgaataagga
     2581 gggaactttt gttgtgcgta atttttcaac taagtgtttt atttgatctg gtgttccttc
     2641 ttcttcagtg ttgatttccc aaatgtaagc agaaggcaca ttatacgctt tcgagaaata
     2701 tttaaagcat ccttcacttg taacaatcat ttttttatca ttcggaattg aagcaaattt
     2761 agatttagct tctttgtcta gagaatccaa tttttcaata tacttatcta gattttcttt
     2821 atagaatttt ttattatcag gatctttttc cgctaactgt ttttcaatat ttttagcgta
     2881 aataataccg ttttctaaat ttaaccaagc atgaggatct tccttccctt tctcactctg
     2941 accctctaag taaataacat ctatgccatc acttgctgca aaatagtctt tgttttcctc
     3001 tttgttcgca tttttaacta attttgtaaa ccaagcattt cctccagttt ccaagttaac
     3061 accgttataa aaaatcaaat ctgcttttga agttttttga acatcttcag gcaaaggttc
     3121 atattcgtgg ggatctttcc caataggtac gatactgtgt aaatcaattt tatcttttgc
     3181 tatattttcg gtaatatctg ctaaaatcga attagtaact actactttta atttttcgtt
     3241 cgaagccact ttatctttgt ctttactatt tattgaacat gccccgaata taaaaagagc
     3301 aatcaaaatc cccagacttc ccagtactgc tttccatttt ctcatcataa cgatctctcc
     3361 ttctttctta aaaatttttg ttttggtgag attagaaaac taattacaaa taagaatgcc
     3421 gctgttaaaa caatacttga tcctgctgct atgttaaaac tataaccaat aaacaatcca
     3481 agcaatgatg aacttgctcc gactgttgca gaaataaata tcatagtttt caagctattt
     3541 gcatataaat atgctgtagc tgctggtgta attaacattg caacaattaa tatagttccc
     3601 acactttgca tagctgttac tgctactaaa gttaatagaa ccatcaatag ataatgataa
     3661 aaactgacat tcatccctat tgcttttgcc attaatggat caaaagaagt gattaataat
     3721 tctttaaaaa atagaactac tataaccaac actaaaaccg aaacgccaat tgtaatccac
     3781 ttatcaatat cttgtacagc taaaatattt ccaaataaaa tatgaaataa atccgttgag
     3841 ctatttgcaa ctcctataag gataactcct agtgctaaaa aagaactgaa agtaatacca
     3901 attgctgtat ctccttttat tacactattg ctttttataa aagtaataat gatagaagct
     3961 aatagaccaa atacaatcgc accaataaaa aaattaatac ctaaaatata agaaattgct
     4021 actcctggca aaacagcatg agaaatagca tcacccataa gagacattcc tcttaaaata
     4081 ataaaacaac ctactgttcc agaaacaatc cctataacta cagatgtaat tagagcattc
     4141 tgtaaaaagt gaaagtcata cagaccatta ataaaatttt gaagcatatt attccccctc
     4201 tccaataaat atagtatctc cataggcttt ttttaaattt tcttcattaa atacagattc
     4261 tacacttccg tgagcaatta gatttcgatt caaaagaata atattatcaa aatacttctt
     4321 taccttgctt aaatcatgat gcacaattag cactgttttt ccctgatgtt tcaatttttt
     4381 taacgtatcc ataattattc gttcactaac taagtctatc cctacaaatg gttcatctaa
     4441 aaatatgaaa tccgcatttt gagcaagaca tcttgctaaa agtacacgtt gaaattgtcc
     4501 tccagataat tctcctattt gatgattaga atattttccc atatcgacct tttccaaagc
     4561 ctttgaaact ctttgccatt ctgcattttt aattctttga aaaactttca ttccggcata
     4621 tgttcccata gatacacatt cttttacttt tattggaaaa gtaaaatcaa tatcagcttt
     4681 ttgctctaca tatgcaatct tcaccagttt tttattgata tcttcattat taatggagac
     4741 ctctccctga tgagggataa tatttaacat tgctttaagc aatgtggatt ttccagcacc
     4801 atttggacca ataatacctg ttaaagcacc ttcttgtatt tttaaagaaa tctcgttcaa
     4861 tgctaaaaaa tcgttataac aaacattaag ctttttcact tctaacataa ctttctcctt
     4921 ctaaatgata atgattatca cgatcatatg gtatacaaaa attatatttt agtcaaccct
     4981 aaattttata gtt
//
LOCUS       DAYYME010000163         4989 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000163 DAYYME010000000
VERSION     DAYYME010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.164"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            331..1254
                     /gene="truB"
                     /locus_tag="ACHBFW_002583"
     CDS             331..1254
                     /gene="truB"
                     /locus_tag="ACHBFW_002583"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFM9318846.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1258..2199
                     /gene="ribF"
                     /locus_tag="ACHBFW_002584"
     CDS             1258..2199
                     /gene="ribF"
                     /locus_tag="ACHBFW_002584"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFM9318847.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2594..3379
                     /locus_tag="ACHBFW_002585"
     CDS             2594..3379
                     /locus_tag="ACHBFW_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9318848.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3392..4243
                     /locus_tag="ACHBFW_002586"
     CDS             3392..4243
                     /locus_tag="ACHBFW_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM9318849.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4367..4819)
                     /locus_tag="ACHBFW_002587"
     CDS             complement(4367..4819)
                     /locus_tag="ACHBFW_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9318850.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
BASE COUNT         1688 a          815 c         1039 g         1447 t
ORIGIN      
        1 tgattaagaa actgaaaaaa taaataaaaa aaaacggaaa aaagactgga agaatggaca
       61 gtcttttttt tcgtttttgg gaagtgaaaa ccaaactatt tacctgtaag ccacccataa
      121 atccaatcaa atggtgcaaa gaaaaaatct tccattctat tccctccttt aaaaaaaaca
      181 taacatgatt tctatttacg tgagcatgaa ttttgttacg tttttattgc cgaaaaataa
      241 aaaccgttca ttatgatgtc aaattcgtca gtaattgatg caatgtgtta taataattca
      301 gttaaatcaa aaatgaaatg gagaaaagac atggacgggt tattaacttt atggaaagaa
      361 cgcggaatga caagtcatga ttgcgtattc aaattaagaa aaatcttaca tacgaaaaaa
      421 atcggacatg ggggtacgct tgacccagac gtagacggtg tgttgcccat ttgtattgga
      481 aaagccacaa aagttatcga atatttgaca gattctggga aaatctatgt aggcgagata
      541 actctaggtt tcagtacaac gacggaagat cgatccggag aacgggtaga aacaaaacca
      601 gtagatatgt ctttatctga agaaaaaata gatgaagtga tggcttcatt tattggtgag
      661 atcacccaga taccaccaat gtactcagcg gtaaaagtca acggtaagcg gttatatgaa
      721 tatgcccgca ataatgaaac agtggagcgt cctgttagaa aggctcagat ttaccgtttt
      781 atccggacaa gtgaattgac aagagacgaa gtgacaggaa ccatttcttg gcgcttcgaa
      841 gtagaatgcg ggaaaggaac ctatgtccgc acattagctg tagatacagg caagaaactt
      901 ggttaccctg cgcacatgtc tgatctgacc cgaacagcaa gtgccggtat gtcccagaaa
      961 gatgctctga cattagaaga agtagctcaa gctgtgaaaa atgggacaat cgatgattgt
     1021 ttgctgccaa tagaaaaagg agtgaaacga ttcaaacgag tagatattac tgatgaagta
     1081 tggcagaaag taaaaaacgg tatgagactt aattatactg tatttgcgct cccagaaatg
     1141 ccgtcagaag aaattgcttt gttttataaa ggcaaagttg tcagtatcta tcagccaaac
     1201 ccgaaagaaa aaaacaaatt aaaacctagt aaagtattga gaaatgagga ataacgaatg
     1261 aatatcatca agatcagaca tccttaccaa tcagaacaaa ttccagaaga cgatgtcgtt
     1321 ttagtactag gattttttga cggcgtgcat attggacacc agaaagtcat aaagacaggt
     1381 atggaaattg cacaaaaaga aggattgaaa ttagcattga tgactttcaa tcagcatcca
     1441 tcgattgtct ttaaaaagat cgatcctagc agcgtgaaat atttgactag ccttaagcaa
     1501 aaagaagaaa aaatggccgc tttgggtata gattatttat atgaagttga ttttacttct
     1561 tcttttgctc atttagcacc gcaggaattt gttgatcaat acatcgtagg gttacatgca
     1621 aaatatgctg tttccggttt tgattatacg tatggaccaa aagacattgc agatgtcgct
     1681 cattttcctg aatatgccaa aggaagattc aaaatcgtca cagtttctaa agaagaagaa
     1741 caaggtgaaa aaatcagctc tacccgtatt cgaggattac tagacagcgg acaagtaaaa
     1801 gaagcagcaa aactattagg ttgcacatat gaggtagaag gggtagttgt ccatggcgat
     1861 gcacgaggca gactactagg attccctaca gcaaatgtca aagtaaagag cacagttcat
     1921 ttgcctaaag aaggtgttta tgtatcggaa ataaaaattg gggaaatttg gtatccagcc
     1981 atgggttcaa ttggacataa tgatacattc ggtgaaggac gggagctgac tgtagaattg
     2041 tatattttgg actttaatca agatatctat ggtgaacatg tcgcagtacg ctggaatcaa
     2101 tttttaagag atcaagtgaa attttctagt gtagaggaat tgattgaaca actcaaagag
     2161 gatgaacaag caacagctga atatttttct cagcaataaa acattatgca taaaatctct
     2221 tgtttatgta taactagcaa tttcaataaa atgggtgata aataagccca ggaaaggggc
     2281 tgtgacaaaa gtcttgtcac agcctttttt tccaaataaa tggtttttca gaagtagctt
     2341 cttcggaaat aagccgaaat ttcacaaaaa tttgagaagt aatttttgta aattacttct
     2401 tatttcttga agctggccac ttctgtcaca acctctttct ttatagaaat aaaaggacac
     2461 tagcaattca tgaaaacgat tgctaaagcg tatcatttgc tttagactat atatcttggt
     2521 gtacactcac tttaagacgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa
     2581 aggagtgggt actatgtata aggaattaga aaacaaagtc gcagtagtaa ctggtggctc
     2641 aaaggggatt ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt
     2701 caactataac tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg
     2761 cggaaaagca gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact
     2821 tgatacggct ttagaaaatt ttgggacact ggacgtttgg attaataatg ctgggatgga
     2881 aaatcagcat ccaacccatg aacttagcct tgaagattgg gaaagagtat taaaagtcaa
     2941 tttgactggc gtttttatcg gaacgaaagc ggctttaaat tactttacag agaatgataa
     3001 aaaaggaaat atcatcaata tgtcttctgt tcatgaacaa atcccatggc caacgtttgc
     3061 tcattacgct gcttcaaaag gcggagtaaa actattcacc gaaacggtag caatggagta
     3121 cgcaccaaag ggaatcagaa tcaatagtat aggacctggt gctatccgta cacctattaa
     3181 tgcggcaaaa tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg
     3241 acgcgtaggt gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc
     3301 cagttatgtt acaggtatta cactatttgc agatggtggg atgactttat atccttcttt
     3361 ccaaggtgga aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc
     3421 agttgggtgg gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga
     3481 gatattagga actggtattg gagccgtgct cgttggctta cttgttcaat tattcgcttc
     3541 tccagctggt atttcagtga ctacattttt gattagttta ctttctggtg cattctgggt
     3601 catcggacaa acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc
     3661 aatttcgact gcattacagt tagtcggaac ctcattgatt ggtgtcttcg cttttggaga
     3721 atggtctgga agtatgggaa aaatcattgg tgcagcagca atcattcttc ttgtatttgg
     3781 ttctgcttta acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat
     3841 cagtctatta gcatcaacaa gtattggtta ttgggtttac agtgcattgc ccaaactagt
     3901 tgatgcaagc ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt
     3961 gatttatgtg attttccgaa aaccaaccgc attttctgaa gacaagagtt ggaaagcaac
     4021 aatagttgga gccatcttca gtgtgtcagc attggcttat attttttcag caaatgaaaa
     4081 tggggttgct actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg
     4141 tatgtacatc ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt
     4201 attgctgatc gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa
     4261 tctcatttat taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc
     4321 gctagaattt ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaagtg
     4381 aaatatctgt ctgcatcaat aatttttcat tgatccattg actaaaattt acgacatgat
     4441 tgaagttacc accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa
     4501 gacggtcttt tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggattt
     4561 gattgattgc tgtaaaatca cgatactgaa ggttgaaacg catcgtgatt ggtgcattta
     4621 gcatgaatcg ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa
     4681 aaagattacg ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt
     4741 catgaagata atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta
     4801 ctgagtaaaa atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga
     4861 tatttgctta atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca
     4921 agtcagaaaa ttctaacttt tctttctcta aagaaattcg ttctctccta aagtgatatt
     4981 atatttaaa
//
LOCUS       DAYYME010000164         4973 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000164 DAYYME010000000
VERSION     DAYYME010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.165"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(236..1438)
                     /locus_tag="ACHBFW_002588"
     CDS             complement(236..1438)
                     /locus_tag="ACHBFW_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9318851.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(1498..1701)
                     /locus_tag="ACHBFW_002589"
     CDS             complement(1498..1701)
                     /locus_tag="ACHBFW_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM9318852.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(1843..2910)
                     /locus_tag="ACHBFW_002590"
     CDS             complement(1843..2910)
                     /locus_tag="ACHBFW_002590"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM9318853.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(2968..3075)
                     /locus_tag="ACHBFW_002591"
     CDS             complement(2968..3075)
                     /locus_tag="ACHBFW_002591"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9318854.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            3238..4050
                     /locus_tag="ACHBFW_002592"
     CDS             3238..4050
                     /locus_tag="ACHBFW_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9318855.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(4424..4615)
                     /locus_tag="ACHBFW_002593"
     CDS             complement(4424..4615)
                     /locus_tag="ACHBFW_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318856.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1529 a         1014 c          775 g         1655 t
ORIGIN      
        1 tgttcagtcg tcctttcatc ttagatcact tccttaaaat gatcataccc gtttcaacca
       61 cataaaaaag gacttaattt tgaaaaattt cccatcaaaa ctaagtcctt tttattatag
      121 ttatgcaaat ttgcttattt tctcgctatt tccgatacat cttgaacagg agcaattcga
      181 ctgaaaacac tggtgcaaaa gcatttattt gtcttatggt agaaccgtgg aaaactcaga
      241 ctggtaataa attgtcattt tttgaaagaa tagcgccctc ttgaacatta tcaatcgcac
      301 ttaccacttg ttgatctgac tttgcatata agtgaccgta agttcctaaa gtgatttgaa
      361 tattcgcatg gcccaatcgt tcttgaatag atagcggttg aatattctgc tcaattagaa
      421 atgcaacatg agaatgtcgt agcgcgtgaa gcttaatcgg tttgacaccc gcttttttag
      481 cagcttcttt taaccagttc gcaaaagaat aaggtggcgt aaatgttccg tccaactgag
      541 caacatattc aaaatttcca atacgttttt gcaagtcata ccactgtctg agttttaagg
      601 aaagcttctt ccccaaccca atcgttcgat aagcacattt agttttggga gtcgaaaagt
      661 accaatcatc ttttgtatta taaatcaatg tcttctcaat cgttatcgtg tttttctcaa
      721 aatctacatt cgaccaagac aaagcttcaa gttctccaat ccgaatgcca gtataaaaga
      781 gcatttcata cacaatcttc cgatggtggg tttgaatctt atcatccttc aattcattca
      841 tcactttttg aaactcactg tgttcccaaa actcaacttt cttattctca gaaggaagcg
      901 aagaaacatc ttcaacggga tttttagcaa tcaccccctc tttaacagcc cgatccaata
      961 caatttttag gtgccctcga atccgattga tataagattt tgataacgtt tcgccattaa
     1021 cttttttccg tgaattacgt gactctgtct ggcttagttc aaataaccaa tcctcaatat
     1081 cgtgacttgt aatatctcga acacgaaaat gatagaaaaa ctgcatacgc ttttcgaaaa
     1141 tgaattgcgc attctcatat gtttgacttt tgacctgtct ctcgtaccat tttacaaacc
     1201 tatccttgta aaactcttcg aaagtcattt gattggtaga acacagttga ttaggaattg
     1261 tcttgttaaa atcatgtgtt tccatgtact tgtcataagc aattttggca tctttttgtg
     1321 aagcataacc tcgtttagtc ttctgaacct ttcttccatt ttcatctata cctagatacg
     1381 ctcggaagaa ccattttttt gtctttttat cttggtaaat atttttaatt tttggcatcg
     1441 ttccattcct ccctgtactt gagaaaagct actttaatag taccgcgctt gtactgttta
     1501 ttccgcaact tgaatgtgaa aaagatgttc aattactcgg gatggcacca caccaacttg
     1561 gcgattatta taaaaatcaa taccttcaat ccttgcaaga tatgccttgc tctctttaat
     1621 cattcgtgca gcttgactag gtttaaagcc catctctatc aaaattgcac gatttaccat
     1681 gaaatcacat tttgtttcca ttctctcacc tcctgattct ttactccttt taaaataatc
     1741 ctctttataa tctattgttt ctcattcgat ggttgtgtta tactcgtctt gtcgaggcgg
     1801 agtacgttgc acaacttgtt gtgtaagcag tactttgctt ttttagtagc ttgttttctc
     1861 atttgatata ctttgtaatt ttcgttcata atactcaacc tcctcctgat aatactccgg
     1921 gtctgccttc atattttgga gaagtttgat ctgtcttgga ttcatttctc cttgttcaat
     1981 aatcaaaggc aacaaggaag aatgaaacgt ttgatcgacc tgtgcaataa aaactaatga
     2041 tggtgaaaca ccatgaatta accaactcat agttttctca aaactttgag gaattgtatc
     2101 taaagaaatg gtgattgcgc cataattgct aataaaggct gcccatttgg catcaacttt
     2161 tgcacctaga tcatttggat cacggtcata aaaacaaatt gctccattaa gtaactcaaa
     2221 gaacagtttg gttaaatcat gggtttttga aataatcttt gccaagcttt gcgctttctc
     2281 ttttcgatac cgtagttcaa agcgattctt tacttttgcc tcttctaaag gaattcctcg
     2341 tcgcttccgt tgttcatggt ccttctgata aaagcaaaaa cgacactgac tcttctttga
     2401 gcctaaatac aaggtctcac catttgattc attgtttatg atatccgtac cgccatgaga
     2461 ttcactcact tgaaaagtgg tccacacatg gcctagtatt accttatgct taagttctgg
     2521 aatgctgaga ctccccacaa aatcatctaa agtaaaatca atccgtgtaa agttcccctg
     2581 ataatctaat accttacgaa aaaactgttg ccaatttgtt tttcgtgttt ttagccatct
     2641 tgctaagtgt ttacagcctt gacctgaaaa ctccatcaca gttcctttaa ccgcatcgtc
     2701 aatggaatac cgaatcgtga ttacttgatt ccacacgtat tggccaatat agccctttgg
     2761 cgcataatcc agttcctcaa aacgttcaat gggcatttca ataatctctt caattagctt
     2821 taaatgttga ttcgagaaaa agtgaatggt tagataatca atcaaaacac ggttaactgg
     2881 aacgacctct tcttgcaaac tatccaacac aagattcaat ttttcttcac ttaattgtct
     2941 cgttcctttt tctactttcg ataataacta tctcgaaatc cccacttttt ctgctaaata
     3001 tcgttgtgtc cacccccttt cttctcgaga aactcgaatg gcttcaccga gatcatgttg
     3061 attaacttgg ttcatcgaca aacctccttc tgctatttac ttgtcaaaga acctattgaa
     3121 cttattttaa gtacattttt attgtacgcc tgtatttatt ttaagtcaat atgttttgac
     3181 tttttttaag tacacacgta tactaaattt aagaacttat cctgaggagg agttttcatg
     3241 gagatagaca agcaagcagt cggcaatcga attcggcaga ttcgccaaga attaaagttg
     3301 agtatggaga aatttggcaa actaattggt gatttaccgc gaagtacagt caataactgg
     3361 gaacgtggga tcaaccttcc aaaaaccgaa acccttcacc aaatcgcaga gataggtcat
     3421 gtcaccaatg aatatctttt gtatggtgat caagagaatc aatacatttt agaaatgctc
     3481 caaaaaaagg caggtaagct tgatcctaag attgaaggtc tcatagtaga cgaaatcaaa
     3541 cacgctgacc ttgttagtga aaaagaaatg gatcgaatga ttgaattttt tattacaaat
     3601 ctcattccac caactgaaca agaccagttt actttccaat gtatcgatga aaaaaagcag
     3661 ctttatcttg gttcaactaa ttttggtaag gaagcccagc tctatttgca acatgataat
     3721 caaaatcata ttctgcacat gatgcccttt accttctcca actttagtgt ggatcgcata
     3781 ctcgtctatt tagcaaatca aaaatcctat tcctattttg ggaaacattt acccaaggaa
     3841 ctaggtgaga aagccatcct tctatactca attaatcaat tgaccagcga tgtgcgaatt
     3901 gccccgttgg tctattcaga agaaaccgct tcttatcagt acacagagga caatcaatat
     3961 ctactagaac agcaatatct ttatctacca ttcgtgatgg aagtcgagaa agaacgcctg
     4021 ttaaatgcag cgtatccatc atcaaaatga ggtgacaaaa ttgataagca cgtgacaaag
     4081 aaaatactgc taggacactc cccaatgggt agttgtcaca gtttcctgtt ctttgttagc
     4141 cccctgttag aaccgggggc tttattcctg cagtccggct acgccttcct gcaatagaca
     4201 aaactccaga tcgggtaatt cgcttgtggc gaaacccgac ctggagtttg tctctgtatt
     4261 ttgaacccta aaaattaatc aaacagactg atattctcat aatcagcgcg aaataagcca
     4321 ttaagtacag ttttcctacc tatttttgat aacccgctgg ttagttaata ttccttttcc
     4381 gagagagata aatgattggt taaatctata tacatctcaa cctttaaaaa tgttttcgaa
     4441 atacttcatc agtacatgtc atacaaatta ccagtgcatt ctcaaattct agttctttaa
     4501 ttgatttcag ttgaggagca gttacttttg gcggaaattt ccatttttgt ccaaacctct
     4561 tcccccgatg aaatttcttt gttgcaatta ttgcaatgaa aaatttttgt ttcaaaaata
     4621 tacataatta cttcctccaa taaaaactat ctacctctca aattattttt ctctaataag
     4681 aaaaacagaa aaggtaataa gatacaacag tgatgattcc taataaagaa aagactacaa
     4741 taatattttt ctaacttatc gccttcaaag aaatagtttc ttatttttca ctaaaataaa
     4801 aaatttattg caaaaaaaat agaagtgaca aaattatcaa tcatttcaag gtaatataac
     4861 tacctgagtt tattatcgat taaaatgcta actacatttg caagagaagc gagagttaaa
     4921 ggtaaatata gaatttctac ttttttagca atagaaatag gaataaaata ttt
//
LOCUS       DAYYME010000165         4968 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000165 DAYYME010000000
VERSION     DAYYME010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.166"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..190)
                     /locus_tag="ACHBFW_002594"
     CDS             complement(<1..190)
                     /locus_tag="ACHBFW_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFM9318857.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHI"
     gene            348..611
                     /locus_tag="ACHBFW_002595"
     CDS             348..611
                     /locus_tag="ACHBFW_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318858.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            635..823
                     /locus_tag="ACHBFW_002596"
     CDS             635..823
                     /locus_tag="ACHBFW_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318859.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(906..1091)
                     /locus_tag="ACHBFW_002597"
     CDS             complement(906..1091)
                     /locus_tag="ACHBFW_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318860.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            1306..1677
                     /locus_tag="ACHBFW_002598"
     CDS             1306..1677
                     /locus_tag="ACHBFW_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM9318861.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(1827..1899)
                     /locus_tag="ACHBFW_002599"
     tRNA            complement(1827..1899)
                     /locus_tag="ACHBFW_002599"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1864..1866),aa:Gln,seq:ctg)
     gene            complement(1945..2373)
                     /locus_tag="ACHBFW_002600"
     CDS             complement(1945..2373)
                     /locus_tag="ACHBFW_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318862.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(2433..3191)
                     /locus_tag="ACHBFW_002601"
     CDS             complement(2433..3191)
                     /locus_tag="ACHBFW_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM9318863.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            3486..3683
                     /locus_tag="ACHBFW_002602"
     CDS             3486..3683
                     /locus_tag="ACHBFW_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318864.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3789..4271
                     /locus_tag="ACHBFW_002603"
     CDS             3789..4271
                     /locus_tag="ACHBFW_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318865.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(4329..4925)
                     /locus_tag="ACHBFW_002604"
     CDS             complement(4329..4925)
                     /locus_tag="ACHBFW_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM9318866.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         1597 a          777 c          961 g         1633 t
ORIGIN      
        1 tgatgtgtgt tattaataca gacgttgttt cccaatgcct aaaagaaaaa atttgataag
       61 taaaatatac agcaggtaaa gaggccagta aaactgaaaa cttctttttt gtccatttta
      121 tgtaatagaa aagaatgctt atcataataa aaaaaggaat ataaaaaaca aattgaccga
      181 tcgcgatcat actattgcct ccgataaata aagtccatga tacaaaataa cactttgtgc
      241 gaaatggcgc aagcagtttt gtggatttat ccggtaaaat ctgtatcgat tttgaaatta
      301 gaggagaaac ttgctataat caaagtgaaa gaaggagtgg gattttcatg gatcatgtag
      361 tgttagaaat cgatttgcat ttgatcaata atattcgggt gatttactat gttgtaagta
      421 acagtgtcga acaacgtgta ctgacgaata aaataaatgg tattttagca aagaaagacg
      481 ttcaccgatt caataatggt gaaggaagtt attatagtat cccagtagaa aaaattattt
      541 atacgacagt caaagtcaga gaagatctag ataccaaaaa agcatatgaa cctattttta
      601 caacttattg attgacagca gatggaggaa aaaaatgaat ccatatgaac aacttattca
      661 agatttgatc gatggaaaaa tcgaaaagat tgatgtaaaa agagaagaat taatggcttt
      721 tcgagaagcg tggctgaagt tggagaatcg taaatatgtg gtgggaaaag cctcactgaa
      781 cggcaatgtg acttatcatt acgatccgac acgtttattt tagtatgaag tattcagaaa
      841 agaaccaaag gaggacgatg cagcagcatt gtcctccttt gattttacaa aagtgaaagt
      901 tttacttatt tgaatagatt tttaaatttg tttttgacac tgtcgatatt tacatctttt
      961 aaaagatttt gggctttctc tttataaggt cctaattgtt ctttatgttc ttcgataaat
     1021 cgttttgcgc catcgaaatt gccgttctta atcatttcct tgactttttg aaataattct
     1081 tcttgattca ttttgaacac atcctttgct atcaacatga ttttttacat ggacaaaacg
     1141 agaaaaactg atttatccac acgtcaataa tatcaaataa cgatcatttt tactaaaaaa
     1201 acactcgatt ctcgcttcta aagtaggaga aaaatctcca acttgggacg gatggaattt
     1261 ctttaactga cccatatact gaatatataa caaggaggta gaaagatgaa agtgattaaa
     1321 tatttgattg gactggcggc gattagtggt attttgctat ttggactgaa atattatact
     1381 gaaggaagtt atggagaaat ccctggtata cttgatcagc taaatccatt agttgaaaaa
     1441 ggagaggtct atgtaaagac acaaaaacca gaggaagtaa atgaatatgg tacagcaagg
     1501 tatgtgcaga aagctgctga tgccaatggg aaagaacgaa cggttgaatt taatggtctt
     1561 tctgtgttaa aagaaaatca ttatttgaaa attagcaata aaggagcaca catagaaacg
     1621 tatgaggaag ttcctagaga aaaagttccg gagaaagcat tagcaattat tgggtaaatc
     1681 agtttttgta ttaagaatac gcccgatttt attattcata atttaattat gtaaactaaa
     1741 tacaagtagt aaaaataata caataatccc cctgcaaaaa gattgttctt ttacaggggg
     1801 ttcatttttt cagcaagcac atcaaattgg gatggcaggg ttcgaaccta caattgcttg
     1861 attcagagtc aagtgcctta ccagtttggc tacatcccat ttattcaaca aaataatcat
     1921 agcactttcc taaaaaaacg caagttattt ttcttgatcg gatagttgtt gctttactcg
     1981 taaaaggcgg cgtactagtt tcttttctaa tccagcagga agggcaatca gttctgctac
     2041 tgacaagacg gtgtccaata actgatattc tttgacaggt tcattcataa tatgatagat
     2101 gtttgctaaa cgttcaatat attcaaagcg agtatactct gttcgagcaa ttagaaattt
     2161 caataaatcg gtggctttct cgtactcttg atctgcgtaa taaacaagag attgacgata
     2221 caatttgatt tccagttcat tgagggtagc aggtctgcga cgctcgttcc actcattaat
     2281 agagacttta ccttgaacat ttcccttgca gtcacggaca aaaataatcg gaatttcatt
     2341 tggatccatt tttttatgtg tgaatttttc cattatctac gctcccttca aaggtttggt
     2401 atcttgagta taagggattt ttgtttgaat tactagcttt tctccaaagt tagcatctgt
     2461 ccacccattg cagtgaaaat cgccgaaaaa tcgttgatcg gtactgctcg attttttaca
     2521 ccatagggat cgttgacata aatattttct ttgtcatatc cagtgactac acaagcatga
     2581 acaagaggag tgactcttac ttctccatta tttgtggtcc aagtcataaa atcagtttca
     2641 tctgggactt caaaattgat agtcacagaa gtccagacag gatttccttt agatagtgct
     2701 tttttgatat catcgagttg agcatcggtt tttggaaaag tgtgataatc cgtttggacg
     2761 atttgctgag caagggaggc aactggggga acatagacga ccatagccca gtctccgcca
     2821 ctgatatcac caacaaatcc ttcattagga tcgccatgag tcccatctgc attgaaaaca
     2881 gggacgtaat ccaattgctc agctaactgg tttttagttg tttcataacc ataaaaagat
     2941 aaaagcatag aaagcgaagt gacttcacag ccattttcta aagaaggaga atcaaactgg
     3001 ctctctaatg gaacatctag taaaacttct gctttctctt ttgtcttttt cgttttttgt
     3061 gttgaacttt tttgtgcagt gttcattgta tgttctggag aatggatatg taggtaataa
     3121 cttgtaccgc ttaataaaag agcagctgtg ccccctagaa acaatagttt tttgaacgtt
     3181 tttttcttca tttgaaatct cccttttcag ctgtttatct ttcctattat aaatgtattt
     3241 ttattggaaa tggaagtatt atattttaat atgtaggaaa taattagttt ttaaggaata
     3301 tgttttaatt ttttgaaaaa aattcttcta aatacaacag ctgttttaat tgtgaaaacg
     3361 aaaacagtac gctataatgc ctgtaaggtt gatttttaac tgtaaataaa aatcaatgag
     3421 ttaaatctcc aaagaaatag cagtggtaat gtggagaaaa gttccagcag tttaggagga
     3481 ttttgatgac ggaagaaatg attaatttag gagaacaata tgcatgcaaa ccaattggtt
     3541 ttacaaaaac ggtcattggt gaagttgtat caaaaatgac aaactgcgcc gtggtcaaag
     3601 ttgcccaatg tgctgcagag gaccaagaat tattagatga aaaagcaagc atggttgttg
     3661 caaaatatga tacatttgaa taaagaaaac aattggacgt gcggcgtgct gcaagtccaa
     3721 tttctttgag aaggtaaatc agtattcttt tcctattttc tggagaagaa aaagcgaggc
     3781 gagttgtcat ggtaacattc tttacggttc tattgttttt attcgttgtt gtgatgtttt
     3841 tatttctttg gctggttcga aaagaaatta tttatcggac tgtgcgaaat cgatgggtct
     3901 acttggtgat acccttctta gtgtcactag tcatttggta tacactcatc agtcatccta
     3961 cagcagatga attggcaaaa gggatcttgt cagcaatgat ttttattagt tttttgttgg
     4021 acagtagagg aatcacagaa gaaggacttg ttttgaatag ctttgacaaa aaaggtgttc
     4081 ctttttctga gatcaataaa attgtattat atcagccaaa aggttctaaa atcgtaaaaa
     4141 tgaatttttt ccgaaacgga tggcgaggac cgatgctgaa attttctgca tcattagaag
     4201 aattagttcc atttttgtcc caacgcttaa acgatgaggc agagattgat attatgattg
     4261 atccagaata aaaacagagg ttgcgacaag atgtttgtca caacctctgt tttttatttc
     4321 aaataaaatt atccaaatac gcggaagcga tcggcatcgt tcatgtattc tttttcctca
     4381 gccggtgttt ctggtgaacc gaatactaat tgcgaacgta gtttccagtt tgaagggata
     4441 ttccattctt ttgcaactgc ttcgtcaata actgggttgt aatgttgcaa gtttgcgccc
     4501 aatcctttat caactaatgc tacccaagta ttagctgttg cgattccgtt tgattgttct
     4561 gaccaatctg ggaagttgtc tgcatataat tcaaattgtt cttgtagacc ttttaccacg
     4621 tcagtatctt tgaagaataa tactgtacca taaccatttt tgaaaccagc taatttgttt
     4681 tgtgtatttg ggaaagcttc tgctggagtc aatgggcgaa gtgcttcttc agtgatttcc
     4741 caaagttttt catgtgcatc tccgaaaagg ataactgcgc gtgttgattg agcattgaaa
     4801 gcagttgggc tttctttaat tgcttctttg atcaaagttg ttaattcttc attagaaagt
     4861 gaaacattac gtcctagatg atagattgaa cggcggtttt tcaaagtgtc agtaaaagtt
     4921 gtcataataa aatttctcct ttgatttgcg ttttgcattt ttttaatt
//
LOCUS       DAYYME010000166         4962 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000166 DAYYME010000000
VERSION     DAYYME010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.167"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHBFW_002605"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHBFW_002605"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFM9318867.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACHBFW_002606"
     CDS             complement(1297..2133)
                     /locus_tag="ACHBFW_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9318868.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACHBFW_002607"
     CDS             complement(2317..3534)
                     /locus_tag="ACHBFW_002607"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM9318869.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACHBFW_002608"
     CDS             complement(3683..3844)
                     /locus_tag="ACHBFW_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318870.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACHBFW_002609"
     tRNA            3917..4000
                     /locus_tag="ACHBFW_002609"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..4962)
                     /locus_tag="ACHBFW_002610"
                     /pseudo
     CDS             complement(4240..4962)
                     /locus_tag="ACHBFW_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1523 a         1024 c          796 g         1619 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta at
//
LOCUS       DAYYME010000167         4933 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000167 DAYYME010000000
VERSION     DAYYME010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.168"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACHBFW_002611"
     CDS             85..972
                     /locus_tag="ACHBFW_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM9318871.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACHBFW_002612"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACHBFW_002612"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFM9318872.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACHBFW_002613"
     CDS             2274..2720
                     /locus_tag="ACHBFW_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM9318873.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACHBFW_002614"
     CDS             2993..4345
                     /locus_tag="ACHBFW_002614"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFM9318874.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACHBFW_002615"
     CDS             4516..4803
                     /locus_tag="ACHBFW_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM9318875.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DAYYME010000168         4838 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000168 DAYYME010000000
VERSION     DAYYME010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.169"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..1886)
                     /gene="pepF"
                     /locus_tag="ACHBFW_002616"
     CDS             complement(78..1886)
                     /gene="pepF"
                     /locus_tag="ACHBFW_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFM9318876.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2062..3060)
                     /locus_tag="ACHBFW_002617"
     CDS             complement(2062..3060)
                     /locus_tag="ACHBFW_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFM9318877.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3286..3939)
                     /locus_tag="ACHBFW_002618"
     CDS             complement(3286..3939)
                     /locus_tag="ACHBFW_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFM9318878.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4271..4669)
                     /gene="spxA"
                     /locus_tag="ACHBFW_002619"
     CDS             complement(4271..4669)
                     /gene="spxA"
                     /locus_tag="ACHBFW_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFM9318879.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1427 a         1012 c          750 g         1649 t
ORIGIN      
        1 aaaatacgtc gactccctta caagagagcc gacgtatttt tttcgtttct attcgagaaa
       61 atcaaaaata gaaagcctta taaacgatcg atcaattctt ctaattcatt tagacgttgt
      121 tcaaacatcg acattgcatc ttcaatatat gcagcctgtg tcatatcaac tcctgctttt
      181 ttcattactt ccacaggata atcgctgtta cctgctttta ggtaagccaa gtagttctct
      241 agtgcctctg gttcctggtt tagtattttc ttcgccagtg ctgaagcagc agaaaagcca
      301 gtcgagtatt ggaaaacata gtaattataa taaaaatgcg gtatccgtga ccattcgaat
      361 ttaatttctg ggtcttcttc gactgctgga ccataatatt ttgcattcaa tttaccataa
      421 ctatcactta ggtattcact agtcaatggc acaccttttt catcttccgt atgcatgaaa
      481 tgctcgaatt cagcgaattg tgtctggcgg aaaactgttc ctttaaaccc atccaagtaa
      541 tggttgagta cataagcccg tacacgaggg tctttttctg tttccaataa atactccgtt
      601 aggatatttt cattcgttgt cgaagcaatt tctgctaaaa agatggagta gtcgccgtac
      661 acataaggct ggttcgaacg agtgaaataa ctatgaacac tatgtcccat ttcgtggaca
      721 agcgtaaata gctgatccag cgtatcatgc caattcaata aaatatatgg atttgtgtca
      781 tagcttcccg aagaataagc accgcttcgt ttccctttat tttcgacaac atcgatccaa
      841 cgttcagaga atgctttttc tacgatggcc atgtattctt cacccattgg tttcagtgct
      901 tctaaagctt tttcttttgc ttcttcgtac gtaaaggtaa ttggagcttc ccctaagacc
      961 ggtgtataaa gatcatacat gtgcagtttt tccacttcta ataaacgttt ccgtaattcc
     1021 atgtatcgat gtaacaaagg caaatgcttg tttaccacgt ctaccaaagt atcgtataca
     1081 ctttcaggaa tatgattatt gctcaaagac gcttctctgg cagagctgta attacggact
     1141 ttcgctttaa aattatgtcc ttttatatgt gtgcctaaag tagaagcaaa tgtatttcta
     1201 aattgttcgt aaacactgta caacccctta aatgctgctt cgcgcaccct tcgatccgtg
     1261 ctttctagca actgaccata cacgccatga gataattgga ctatttcacc attttctcct
     1321 tcaatcgttg gaaaaactag atctgcatta ttcaaaacag caaatgtatc acttgaagca
     1381 tcaaagattt cacctgctcc agcaagaaga gattcctgct cagcagataa gacatgggct
     1441 cgattatcta ctatttgctg gatataatgg cgatagactt ccaattttgg ttcttctttg
     1501 aaatattgcc aaatctggtc atctgacaat tgcaatattt ctggttcaaa ccaggaaacg
     1561 gcttcactaa ctttcgaaaa cagactgctt gctcttgcat aaagcgcttg gtaatctgta
     1621 tttccagtgt cttgatcgtt tttaaggtgc gcatatacat aaatgacttc agtttggcga
     1681 taaaccctca atacgaattc aatcgcattt aaaaattgag aagcgccttg atcaagtgtt
     1741 cctttgtgtt tttcagattg ttttaactct tctgataatt ccaaatattt ctcatcaaac
     1801 tcttggtcgc ttgaaaagat cttggtcaag tcccaagtta aattttcagg caattcttct
     1861 cgttttggca actgctttac ttccatgaaa tccactcctt ttcttccctt agctctatct
     1921 atcaaaatag atcatagtct aacatccaca tgtcacagca tctaactttt ctactcattc
     1981 atgtgtctgc ttccatcaat tttagcataa ctaaatcttg ttttacatag aaaaccgatt
     2041 cctagcctat ttttatactt actatttttt caaagaagac aagtgtatgc attcgttaaa
     2101 aaactgctga tagaccatct cttcatcaat cataggaaaa agccattccc ttttatgttc
     2161 ttttatttgg caaagccatg agttgaaatc aggaactttc tggttttcca aggcttcttc
     2221 gtacaacatt cgataaagaa aaacctgttc ttgtaaataa aagaaaaacc gacttttttg
     2281 atagatccac gatgatagat gcaaaagatg tttatgacgc aagtagcatt gttcttgtat
     2341 ggatactgta ttcttttgct tgtgaataag ttggcgtgtc agccatttct tcccatcttc
     2401 actcactttt tctatttttt tcatttggtt cattttaatt tcgttactag tgaatatttc
     2461 tttaaggtcc ctactagaaa aagtccagca gctactgcaa aaggaaatat gccctgatac
     2521 agtctctgta atatgatgat acaaataaag cttttgtttc ttgagatcag ctttccagca
     2581 atgcactcct ctttttctat tatagtagca gcaacttttt tctatcaatg agaattgcga
     2641 ttgtccaaga aaagaattac caagtatcca ccatacttgg taactgttat tcaaataatt
     2701 ttgggttctt tcaataaatc tctggtgaga cagtctagaa cattggattt ctattgctag
     2761 attcgccaac aaaatatccg gcctttgccg caacctcggc aaataggctt ccaaaaatac
     2821 tggttctact gactgtccca gccaatccat gaagcattcc tttaactgta agtgctcttt
     2881 tgattctctt tcactaaaga ccgaacagtt atctgatttt tgatgggcaa aatgagcaca
     2941 ttttttcttt ccctttttca aaatcactct ttttcgacag ccacgacaat aatatttctt
     3001 tcctttttct gcatgtttag caaaacagca cttattatat tcattcattg caagcagcat
     3061 ttttctccct ctgaacttat ttacgtctaa gaatttattt acacgaaaaa agagggcgtg
     3121 aaaaagctat tctgcatcaa gtaataagaa cagctaaaca aaaatagttc ccaatatttt
     3181 tcgtttggtt gggcttgtta ccgcagatgc aagcttttga acacatttat acgaaaaaaa
     3241 gactgcgaca caatttttgt cacagtctct tactataatg ctgatttata caaaataaaa
     3301 tttcgttcgc tcgatagcat tacgcgccat cagacattgt ccatattcat tcaatgtttc
     3361 tggtgtacga tcgctctttt cagcaaactc caaaattcga gcaatctgat tctctacatc
     3421 cgcttggttg agattttcca tccagaagtg aacgactagg taatagcgct cttcgaataa
     3481 atacaaatca gtccaagcag actctagttc gacatctgtt gcaagttgga tcacttgttc
     3541 aaaatcttca aaagcaaaag ttgcttgtct ttcctgttct tctagggcgc ttccagataa
     3601 ttcttcctcc ggatcgctgc caataatctg ctgacgaaga aagtcactca catcttctga
     3661 atcaacagta gaatcttctg attgagaaat ttcttcatcc ggaagatttt tactgataaa
     3721 cagttctaat ccgtctcctt ttggcaaaac ttggaaagtg acagcttcgc ttcctcggaa
     3781 ctcctcttcg atatcaactt cttccaaaat actataaaag aaattttcga tttctttgtg
     3841 attgccaagt aaatccaaaa atgaaacgcc ccgttcctct aaatcttcat gaccaattag
     3901 tacacggatc gtattttcat taatatgttc catttccatt ttgctacacc tcactttgcc
     3961 acaatgttta atatagattc attgtagcga aatcagctta aatgtaaaga aaaaaaatct
     4021 cgctgttaag cgaatttaat aaaaccagcg gatttttcaa cctttcaagc tatcttattt
     4081 aactataaca tttttggcag acgaatacta tatagccgac ttttcagcta attttccctc
     4141 tctatttttc gtgcaatcat catcttctac taccttttta tacatctaat caagaatact
     4201 ttttgtaaaa acaaaaaaac ttggaaaaac tatctttgta atagtctttt ccaagtttcg
     4261 tattatcctt ctataatcca gccatcaatt gtgcttgacg taattctaat tgtcgtacat
     4321 cgcgcggtag gaaccgacga atttcatctt cattaaaacc aacttgcaga cgtttatcat
     4381 caatcatgat tggacggcga agcaatccag gattgttttg cacaagttcc aataattctt
     4441 gtaacggcag atcatctaaa tccatgttca gtttttggaa tactttggaa cgtgtcgaga
     4501 tgatttcttc tgttccgtct tctgtcattt gtaaaatcgc ttttaattca gaactgttca
     4561 atggttctga aaaaatatta cgttcaacaa atggaatctg atgttcttgc aaccacgcac
     4621 gagcctttct gcaggaagta caacttgggg aagtatataa tgttaacaac gtgcaccact
     4681 ccttttcgtg ataagaatca cttttttgta acagaagatc ataataatct cccatctgtt
     4741 tctattatac ataatcaact tttaaatagc tacctttttt tcataatttt tttaaaaaaa
     4801 tgaaagttat ctaaggttaa tatacagtaa aaccagca
//
LOCUS       DAYYME010000169         4593 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000169 DAYYME010000000
VERSION     DAYYME010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.171"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            189..2858
                     /locus_tag="ACHBFW_002620"
     CDS             189..2858
                     /locus_tag="ACHBFW_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFM9318880.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2949..3563)
                     /locus_tag="ACHBFW_002621"
     CDS             complement(2949..3563)
                     /locus_tag="ACHBFW_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9318881.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3683..4222
                     /locus_tag="ACHBFW_002622"
     CDS             3683..4222
                     /locus_tag="ACHBFW_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318882.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGEMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4219..4557
                     /locus_tag="ACHBFW_002623"
     CDS             4219..4557
                     /locus_tag="ACHBFW_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFM9318883.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1424 a          790 c          859 g         1520 t
ORIGIN      
        1 aaaattttta agggtgggac ttttgattcc tacccttttt ttgcgtttat ttttgttata
       61 cgaacaaatt atgaagttat ttattctaaa ttttattttt ttgtgaaaat tagactcttt
      121 ttaagcagag aaaaacaagg atatgataaa ctgaatacaa cttcatagaa atttggtgga
      181 aaataaatat gaaaaaaaat ttaaagtcat tctttagaga aaacggacgc tacgcagcag
      241 ctagtttcct gattccgttt ttgattttag cacttgttta tctaagtatc gggatctatc
      301 ctggaagcag cagaagtatt ttggcaagtg atgccttttc acagttttct aattttcatg
      361 ccagcttccg aaatgttctt cttggaaagc aaagtatttt ttatacatgg aatgcttctc
      421 taggattgaa ttatttatct ttaatttctt actatctagg cgggatcttc acgccactgg
      481 tgttcttttt tccaaaccag atgatgccag atgcactgta tgttctgact ttgcttaagg
      541 tcggctgtgc cggattggct ttttggtttc ttgcacgtac gttcaagatt cctcgctggg
      601 ggcatgttgc acttagcgtc agttacgcat tgatctcatt tatcactgca cattcggaga
      661 tcatcatgtg gctggatgca tttgtttatc ttccattagt gattttgggg attcatcgtg
      721 ttatggatct taaacggccg aaacttttat ttatcagtta tttactcttg tttttatcca
      781 gtttttatat gggttttatg attggcgtat tttctttctt atattttatc gcccgtctat
      841 tgacaaactg gtcagaatat aaaaaaacga ttgttcctta tggtatcact tctttactgg
      901 caggcggcgc ttcaatgatc attgtattgc cagcagtgct ggatcttcgg tcaaatggcg
      961 aggcactgag tcagatcact acgttgaaaa cagaagcgac ttcttatctg gatctaatca
     1021 tgaaaaatat gattggtgtt tatgacacga caaaatacgg atcgattccg ttcgtttacg
     1081 caggtctgct accattagtc ctctgtctgt tttattttgc cagtcggaaa gtaccgttga
     1141 aaaataaatt tttgtacggc agtcttttcg ccatacttat cgcaagtttc tatcttgttc
     1201 cattgaattt gttctggcat ggtatgcatg caccgaatat gtttttattc cgttatgcct
     1261 atctgttctc tttcttagtg ctcctgcttg caggaaaagg atgggaaaag atagaacaag
     1321 acgatcgtgg gctgttgtta ggaatcattt tggtcgtagc tgctctcttt gcggtggcat
     1381 ggggcatcaa acctgaagga agctacacct atgtgacgat gacttccttt gtattgacag
     1441 tggttttctt agggatttat gcttttgctg tgggatttta tcagcataaa aaactttcat
     1501 tcaatcatct gagtatattg ttattattga tcatgtctgc tgaagcactg gtcaatacga
     1561 acagtatgat ccatggcatt cttgatgatt ggaattatgc ttcccgcagt ttatatactg
     1621 atccttatcc tgacttgaaa gctcttgtaa atgaaacaaa aaaagattcc gactcgtttt
     1681 accgcttgga aaatctggat ccagtatctg caaacgattc gatcaactat ggttacagtg
     1741 gtatcagttt gttttcttct ataagaaatc gtaattcttc ctcttacctg gataaattag
     1801 gtttccgttc aagagggaca aatttgaata tccgttataa caataatact ttgctaatgg
     1861 atgggtttac aggaatcaaa tataacattt ctaaaaacga aagcacgttc agtaaatatg
     1921 gcttccgcca agctggtcaa agtggcgact atacactgta tcagaatgtc aatgcattgc
     1981 cactagcatt tactgcaccg ttgtccgtta atgatgttga gcagccagtg acagataatt
     2041 tgaccagtca gacaaacttg atcaatacat tgtctggttt aaatgaacag tattttacat
     2101 tctatacacc tactttgaag aatcagttga atacgacgat cacaaagaca acgactggcg
     2161 tcacttactc agaaacagca gataaccagc caaaagagct cacttgggaa gtccatgtgc
     2221 ctgcaaatac acaagcttac ttaagtttgt tcccaaccaa ttttgcacaa ttagaaagtt
     2281 ctagtgcgac gatcactgtc aatggtacat cgcgaaaaac gcaaatcaac atttctggtc
     2341 aatactatga tcttggctat tatcctcaag agacaacagt gacatttact gcaagttttt
     2401 atggaacgaa agaagtaagt ttcatggagc ctaaagtcgt tggtctagat gttgtagcct
     2461 atcaagccgc aatgaatcag atcaaagaaa aaggtgtcga aatggaagcc actggcagaa
     2521 aagcttctgg gactgtaaat accgctgaag attccatgtt ggtgacaact attccttatg
     2581 acaaaggatg gaaagctaaa attgatggga aaccagtcaa ggtcagcaat ttcaaagacg
     2641 cattcttgat gataaaagtt ccagcaggta aacatactgt cactttcagt tacttgcctg
     2701 aaggatttac gataggtgca atcctttttg tcctatgtat tgcattgttt attctttatg
     2761 tctggtacct tgaccgcaat cgcccctttt atggcacgat tgatccagca atagaagaaa
     2821 aaccgcgaaa tacacacaga agaagacgaa gaaaataaag aaacgatgga atctgtgtca
     2881 ctcttctatt taaatggcaa aaatttcaat ctttaaatga caaaaaccac ctgatttttg
     2941 gtggtttttt atatacctat gactaaacta tctcttgctg cttcgacaac ttctacagtg
     3001 atcttatcca tttgattgat tcttaaaatc cgatcaatct gcgcaaataa acgatgaata
     3061 agcctaaaat ttccttttgt gatcttaatg atagttgtga tagcctcata atcagaaaaa
     3121 tcttctagct tgatatcaat tcctaaatct gcccatttgt attccaaaat atgatgtgtt
     3181 tcgtccttac taaggttatc aaattcatgt gcaaatccta tgcgagagta aagctgcgga
     3241 taacgagaca atcttttttc aataccaggc ataccgataa gaaccattgc cagatcattt
     3301 ctatcgtaaa tatctcttag ctgttctaaa tgctgaagct ttaaacgatc aatttcatcg
     3361 acaataatta agtcaattgc ttcataggtt ttaggcacag gaggatcttc ttgattgtat
     3421 tttagtacca tatgcattgc tttagtcaga cctattctac tagttattgt gttaatatcc
     3481 gaactgacac gagacatctt ttcagctggt acagtataaa aaactttatt tacacttaat
     3541 atttcatctg ttgcatttat caagaacaag aaaaagtctg aatattcaaa aaaaataacg
     3601 gtgtacattt ttaaaaggag atgtataatc gagatttaga aagcgttttc ctcaaaattt
     3661 aatgaattag gaagtgatta ctatgaaaaa attatttagc gcacttttta ttctgttatt
     3721 tggctttatt ggtattggga cttctggaaa tatagttaaa gctagcacga tggttgaaca
     3781 aacaagtgaa atggcaaaaa cttttagtca attaacacca gaagaacaaa gttacttcaa
     3841 atccaaagga tttagtgata aagatacttt ttacactcaa aagatagagc ctcaagatcc
     3901 gaaaacacgt ttttctgcaa atgtattgcg gttaaccgca tctactaaaa aaattactaa
     3961 tacgcagggg cggacagaat atattataac aagtcccaat tcggcgatgt atcgtatttc
     4021 tacacgcttg agtttaggta gtgcaaaatc atatactcaa acagtaaatc tagctaattc
     4081 ttatggttat ggtggagaaa tgtattttac atttactggg aaaagacaat attacagtgt
     4141 aaaattagct atgcaagttt ataatacatt tggtgtaggg tcagtctctt gcactgcggg
     4201 aggattgaca ataggaaaat gataatttct tactatgtct taaatagaag tgatgatgaa
     4261 ggactcttcg tttttcaatc attacatgag gatacgatgc taaaagagtt ttataatcgg
     4321 attaagaata aacagataga aacaaattta tattttttaa gtgactctat cttgcctttt
     4381 attagcaacc ctaaaattct taacttgttg cagacaaaag atttgaaaag tttaccgatt
     4441 attgtcattg atgaggaaat tgtaaaatat ggcgcttatc taagcgtaga agaattagaa
     4501 aaattaacta acattggaat gagtattcag gaggataatg gggtacatgg aacatagaat
     4561 gaatatgcac agttgagaac ggaatttgac att
//
LOCUS       DAYYME010000170         4560 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000170 DAYYME010000000
VERSION     DAYYME010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.172"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..482)
                     /locus_tag="ACHBFW_002624"
     CDS             complement(12..482)
                     /locus_tag="ACHBFW_002624"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFM9318884.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="ACHBFW_002625"
     CDS             complement(641..1099)
                     /locus_tag="ACHBFW_002625"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFM9318885.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="ACHBFW_002626"
     CDS             complement(1270..2415)
                     /locus_tag="ACHBFW_002626"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFM9318886.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="ACHBFW_002627"
     CDS             complement(2428..3345)
                     /locus_tag="ACHBFW_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFM9318887.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="ACHBFW_002628"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="ACHBFW_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFM9318888.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1422 a          836 c          865 g         1437 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga
     4501 aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc
//
LOCUS       DAYYME010000171         4480 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000171 DAYYME010000000
VERSION     DAYYME010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.173"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..933)
                     /locus_tag="ACHBFW_002629"
     CDS             complement(<1..933)
                     /locus_tag="ACHBFW_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9318889.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILP"
     gene            complement(935..2374)
                     /locus_tag="ACHBFW_002630"
     CDS             complement(935..2374)
                     /locus_tag="ACHBFW_002630"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFM9318890.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2559..>4480
                     /locus_tag="ACHBFW_002631"
     CDS             2559..>4480
                     /locus_tag="ACHBFW_002631"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFM9318891.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEG"
BASE COUNT         1408 a          773 c          750 g         1549 t
ORIGIN      
        1 tggtaaaata taatccttat ttgtcttttc gcctttaatt tttttcagaa gaatatcaac
       61 aataagactt gcaatttcat caattggttg tcttattgtt gtcaattgtg ggaaaaattt
      121 ctcaataaaa gaggtaccat cgtatccaat aatttttaaa tcttctggga tattaagttt
      181 aagttgatga gcaattttca tagtgagaat ggctgttaaa tcatcagaaa caaaaatacc
      241 atctggtttg tgatttataa tagttgactt tatttccatt tcacgacgga tagtagacag
      301 attattagga attttgaaaa tttttccatt tggattttga aaagaaaaac ctagtgcacg
      361 taattctgtg ggagaatccg tgttatcatt tcctgtaatc ataatggtat tttgacaacc
      421 atttttttgt aaagtcttag ctgcaagttt tcccccttca aagttatctg aagaaacaat
      481 ggaaacatta ggagctaaat ttctatcaaa agcaacaata ggtgctctca cacgctcata
      541 atcagcaatt ccaagattat ggcttgatga gattatgcca tcaacttggt tggcttcaag
      601 catttcaatg tattctctct ctttagcagg atcattttga ctattgcaaa taatcgtttt
      661 atatccatgt ttaaataact ctatttcgag atactcaatg agttcagagt aaaagatatt
      721 actaatatta ggaaaaataa gaccaataag ctgaggtgac tttccttgaa gtccacgtgc
      781 taaattattt ggtttataac caagagtttt catagcttca tgaacttttt gttttgtttt
      841 ttctgataaa tatcccttat tattaatgac gcgtgaaacg gttgttgggc taactccagc
      901 taattcagcg acatcagtta attttgctac cattttaata ttttaattca aagtattttc
      961 cagtaggttc gcctgatttg atttgaatac cattttggtg attttcaggg aatacacgac
     1021 tagtaaacac tttttctcct ttattaataa aaatctcaat gattgagtta tctacaaaaa
     1081 tgttagcagt tgtttcttta gtatcaattg agcattcacg aatcgtacca aagtcagtgg
     1141 catattgaat gcctgctttg ctgcgatcta aaataatttt tccttctttg gtatctacaa
     1201 tcaagctaag tccatttcct ttactatcag agaaaagaag taactccgat ttctgatttg
     1261 gaggaaaagt tagttcaagc tcataagtat tatttgtttc tgcttttgct gtaaattgtt
     1321 cagaactagc acgtaatgat gtaatagctt ctactggata ttggtaaagt ttaccatctt
     1381 ttattgaaag ttcttttaca agactcattg ctccctgata atcatactta tcagttggat
     1441 aatcgatatc aggtagtcca atccaactga caatcagagc acgaccatca ggagtattga
     1501 atccttgagt cgcatacgct tcaaatccat aatcaagatt ttgaatttct gaagcgccca
     1561 caagttttac tttttctatg tcaagcgatt gacagatctt aaaagtattc ggatagatat
     1621 tcccataatt tagctcagat ttatccaacc cttgaggaca ataaatgaga acaggttttt
     1681 tgtctacaaa tactagattt ggacactcaa tcatgtattc tgaaccagtg ccgccaaaat
     1741 caaggttgcc aacttcctcc catgtttcca cgttattgtc atccgctttg taaagtttga
     1801 taaagcctga tttttctaaa ttttgtgctc caacaatcgc atacaattgt ccttcataat
     1861 taaacatttg tggatcacga aaatgatcag ttacatcagc aggtttatga ataagaacat
     1921 tctcaccttt cgtaatgttg tagtctttat ccatccatgc tccgatttga agtgtatcac
     1981 gttcccaatt ttcatttcga acgtttcctg tatagagtaa gaatagattc ccctctattt
     2041 catatgcaga accggaataa gcgccgtggc tatctcgttt tgtgtctgga agaagttctg
     2101 ctcctgttac atgaaaatga actaaatcat ctgattcatt gtgtacccat tctttcaacc
     2161 catgagcagc tccaaatggc caattttgat aaaaaagcgt atattttccg ttaaaaaaag
     2221 aaaaaccatt aggatcgttt aacaatcctg ttttaggttc gatatgatag ctagcacgcc
     2281 aaggtgatcg ttttacatta tccttaattt tatccatctc atctttagtc caatcagaat
     2341 aagcacgata tcgtatttct cgaggtaagt tcatatttta ctccttcaat ttatcttttt
     2401 acaactagta tggtaaacgt tttccgttga aaaatcaatc atttcattta aaaaaataaa
     2461 ctatatgaca aacgtttgac atatttttaa gatatgctaa aataaatttg catttcagtg
     2521 aaccgttttc acgtaaaaat aaaaaaggag tgattcgaat gaattacagt aaagtagctt
     2581 ccgacgtaat acaagcggtc ggaaaagata accttattgc agctgcacac tgcgctacac
     2641 gtcttcgtct cgtactaaaa gatgacacga aagtgaatca aaaagcttta gacgaaaatc
     2701 cagatgtgaa aggcacattt aaaattgatg gacaatatca agtgattatc ggtgcaggtg
     2761 atgttaattt cgtttatgac gaacttatca aaaaaactgg actttcggaa atttcaacag
     2821 atgatttaaa acaaattgct agtaaagata aaaaattcaa ccccattatg gcattaatta
     2881 aattattatc agatattttt gttccaatta tacctgccct tgttgcaggt ggtttattaa
     2941 tggcattaca aaacttttta acagctgctg gactttttgg accaaaatca attgaagaaa
     3001 tgtatcctgc gattgaaggt atttcctcaa tgatccaact gatgtccgct gcaccattca
     3061 tgttcttacc aattttagta ggtatttcag ctgctaaacg ttttggagca aaccaatttc
     3121 ttggtgctgc aattggtatg atcatgacaa caccagatct tggtggctca actgaatatt
     3181 ggaatatttt tggttatcat gtttcacaaa caaactatgc ttatcaagtt attccagttt
     3241 tagcagctgt ttggatatta tctatattag aaaaattctt tcataaaaaa ttgccatcat
     3301 ctattgactt tacattcacg ccattattat cagtaatgat tactggattt ttgactttca
     3361 cagttattgg ccctgtaatg ttattgttgt ctaatggaat tacagacatt attgtttggc
     3421 tatataacac tactggattt attggaatgg gtatttttgg aggaacatat tcacttatag
     3481 tcatgactgg tttacatcaa tcatttccag ctattgaaac tcaattactt tcagcgtgga
     3541 caaatggtat cggacatgga gactttatct ttgtagttgc atctatggca aacgttgctc
     3601 aaggtgctgc aacatttgcc atttggttct taactaaaaa ttctaagaca aaaagtcttg
     3661 cttcctctgc gggtgtctca gcacttcttg gaattacaga gcctgccttg ttcggagtta
     3721 acttaaaata ccgctttcca ttcttttgtg ctttaattgg ttcaggaatt gctacagcag
     3781 tagcaggatt acttaaagta gttgctgttt cacttggttc agcaggtttc ttgggatttc
     3841 tttcaattaa tgcaacatct attccatttt acgtattgtg tgaattgatt agctttgtag
     3901 ttgcttttgc aattacttac ttctacggta aaacaaaagc tgctggtatc tttaaagcag
     3961 aagaaattgc tgaacaaaca ccagttaatt cttcaaaagt agcaacaaca gatccaatat
     4021 ctactgatgt tgaatcaaca caaacaaaag tttcatcaga aacaattgtt agtcccattt
     4081 ctggtaaagt gattagtctt tcttcagtaa atgatccagt cttttcatca gaagctatgg
     4141 gaaaagggat tgccattaaa ccaaatggaa acacggtcca ttctccagta gatggtacta
     4201 ttcaactcac gtttgaaact ggacatgctt atggtattaa atcaaataat ggtgctgaag
     4261 ttctaattca tattggtatt gatactgtat caatgggagg aaaaggattt acacaaaaag
     4321 ttactactaa tcaaaaagtt agaaaaggcg atattcttgg agcatttgac cgttcaatga
     4381 ttgagaaaga aggtcttgat gatacaacaa tgattattgt gaccaatact tcggattatt
     4441 cagaagtaaa tatagtagct gaaaatacag tcactgaggg
//
LOCUS       DAYYME010000172         4424 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000172 DAYYME010000000
VERSION     DAYYME010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.174"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..1351
                     /locus_tag="ACHBFW_002632"
     CDS             17..1351
                     /locus_tag="ACHBFW_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9318892.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            1392..2303
                     /locus_tag="ACHBFW_002633"
     CDS             1392..2303
                     /locus_tag="ACHBFW_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9318893.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            2300..3154
                     /locus_tag="ACHBFW_002634"
     CDS             2300..3154
                     /locus_tag="ACHBFW_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9318894.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            3151..3768
                     /locus_tag="ACHBFW_002635"
     CDS             3151..3768
                     /locus_tag="ACHBFW_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM9318895.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            3770..4288
                     /locus_tag="ACHBFW_002636"
     CDS             3770..4288
                     /locus_tag="ACHBFW_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM9318896.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         1324 a          753 c          898 g         1449 t
ORIGIN      
        1 aatttggagg taaaatatga aaaaattatt gtttacaggg ttgctggcaa gttcagcatt
       61 gtttttatta ggaggatgcg gatcaagtaa tgcaagcgat gccagttcaa gtgcagcaaa
      121 ttcttctgaa aaaacaagtg atggaagaaa aaatattgat ttctggtcct tttggggatc
      181 cggtgcacga aaagaagcta tcgaagaaat cattgaggat ttcaatcaat cacaaaacga
      241 gatccatgta aattataagt atcagccatg gggtgacatc tggactaaat ctttatctgc
      301 aataacagct ggaaatgcac ccgatgttat tgtacaggac ataaattccg ttgcccaaag
      361 agcagaagca cagcaagcaa ctaatctctc caaatatgta gaaaaagaca cttctaagga
      421 tttttatccg cagctttggg atacagtaga atatgaaggt gatccttatg cgatcccatt
      481 caatacagat acacaagtca ttttttataa taaaaagtta tttaaagaag ctggaatcag
      541 tgaacaagat ctaccgacaa cttgggatga attagaagaa acagcacgta aactagataa
      601 aaaagaagga aaagaattta cacgcatcgg tttttatcca ttatggaatt taggggctga
      661 tgtatgggcg ctaaacgcag ataacgggac tagttggttt gacaaagatg aaaaagtcaa
      721 gattgatact aaaaataaag tggaagcctt ggagtggatc caagactggc aagattatta
      781 tgggaaaaat acgatcaata aattagaagc cgaatttggg tctggtgtat ctgatccatt
      841 tatttccgga ttagtagcca tgcgtgccca aaatatcaat tattactcta gtttgatgga
      901 aaatgcaccg gatgattttg attttggcgt gatgcagctc ccagaaaaag aagaaggttc
      961 aggccattgg tcgtggggtg gcggattcgt cttggaagta ccatacggag caaaagatcc
     1021 tgaggcctct tatgaattca tcaaatatct ttcttctcca gaagtacaag aaaaattcgg
     1081 cgaaaaaagt tttgatatca tggcgagcaa gacagccaat gagaatttag tgaataatga
     1141 caacctaacg gataaaggaa aaatgatcta tcaaatggcg gacgaaaact ttaaaaatac
     1201 ggtgatcaca ccagttcctt tagatgctcc ggattacacg acattagtga atgaacagat
     1261 tgaccagatt cttttaggaa caaaatcacc aaaagaagga ttggcagatg cacaaaaagc
     1321 agtagaaaac ttagtcaagc aaaatcaata atccattttt gactgtctag gtacagagga
     1381 ggcaagaaaa tttgaagcgt ttatctatcc gaaagaaaaa agaagcaaga tgggcctatt
     1441 tatttatctc gccttttatt atcgggttct tggtatttat gctagggcct atgctttttt
     1501 cagttattgg tagtttcacc gattataatt tgacatccaa gatgaatttc attggtctag
     1561 caaatttcaa aagaatgttc gttcacgatg atttattttg gaaatcgtta tacaacaccg
     1621 tttatttcgt catcttcaat gtaccgctga cgacgatttt cagtgtgttt ttagccacag
     1681 tactcaatca aaaaatcaaa ggaatcagtg tattccgtac agctttctat ttaccggcag
     1741 tcttatcagg agtagcagtc tacatcttat ggatgcagct gctttctcca tctgcaggat
     1801 tgatcaacac atttctgggc tggttccata tccctggacc agcttggctg tttgatccac
     1861 aatggacgaa acctgcgttg attttgatga aaatatggag ttctggagga gcaatgctac
     1921 tttacttagc tacattacaa aatgttccta gatctttata tgaatcagca gaaatcgatg
     1981 gagcaggcgt ttggggaaag ttcaaaagta ttactttgcc attaatcact cctattattt
     2041 tttacgatgt gatcacctct acgatcggtt ctttccaaat ttttcaagaa gcttatgtca
     2101 tgacgaaaaa tggaacaggc ggacctgcaa attctttgct tttttataat ctgcatatgt
     2161 ggaacaaggc gttcgtagct tttgatatgg gttatgcgat ggctatgtca atgatcctgt
     2221 ttctgatcgt gttggttctg acgtttgtca atttgaagct tgcacctaag tgggtctatt
     2281 attctggagg tgacggcaaa tgatggatta tttcaaaaag accagtgtga atcggcaaac
     2341 aaaaaaaata ctaagttatg tcctcatgac agtaatagga atcattttga tcattccatt
     2401 gctttggatg gtgtttactt cattgaaacc aatggaagaa atcgttcgtt atcctccgac
     2461 ctttttccca gaaaaaatcg tatgggaaaa ctatctggat acgattgccg cctttccttt
     2521 ttggcgctat gcaagaaata cattattcat cactgtgcta gtagtgatcg gtaatgtcct
     2581 ttcaaattct tttattgctt atggatttgc gaagcttgat tttcctggga aaaagctgat
     2641 gtttgctttg gttctttcta cgatgatgat cccggggttt gtaacgatga tccctcaata
     2701 tgtattattc tccaagatcg gctgggtagg tacttatttg cccctgatcg ttccatcgtt
     2761 cttcggcaat gcgttcaata tatttctgat gcgtcaattt tacttatcga tcaataatga
     2821 attgatcgaa gctgctgaaa tcgacggagc gaatcatttg tatatttgga gtcatttgat
     2881 gcttccattg acgaaacctg ccttgattac cattgcgatc aattccttca acgcagcttg
     2941 gaatgatttc ttaggtcctc tgttatatat ccaagatcaa gaaaaataca cgttacagat
     3001 tggcttgcaa gtttttcaga accaagcaac gacacaatgg aattatctga tggcaggagc
     3061 aacacttgtc ttgatcccga cgattttgct gttctttttt gcacaacgtt atttcattga
     3121 agggatggat ttgacaggag gttcaaaagg atgatgaatc gatcgattgg taaaaaagcc
     3181 aatgattatt tcttagaaat cattgaatgg attccacgtc ttatccattt acagctacta
     3241 tggttcctgt gcgtcttgcc tgttttcaca ttaggaactg caagtcgaac agttctgtat
     3301 atgatttatc attacaaaaa aaatgaagag aaaaagctcg gttcactatt ctggaaaaag
     3361 tttcgtgaga acttctctct ttatggaaag caagatagtt tagcaagtgt ttattttctt
     3421 cttctattga tcgattatca gattttccgc tattggggtg ggggaattgg ctatacattg
     3481 atgtatacta cgttgttttt gcttctatta agcgcgcttc tctttagtta caaaatcttg
     3541 cttcaactgg aacaaaaaga agcttcttgg attactgcat ttttcttgtt ttttaatgat
     3601 ttcaaaagcg cgcttttcta tttggcaggc acgttgatcc ttcttgtcgc attctggttt
     3661 gcaggaccaa tctattttgc tttattaggt ttcagtttct tgttttattt tcaagtcctg
     3721 ctttttatcc atcgtacgca agaaaaggtt cttaaaaagg aggaataaca tgttgcgttt
     3781 gattgctaaa atcgatgata gtattatcaa caagttcgcc aagcaacgga aaacggatag
     3841 tcgaaaaatc aattttttga aatttatcag taatatctct aatggtggtt ttatttggtt
     3901 gctccttgct gctgttttag tcttgatacc tgatactaga gaatatggtc tagccattct
     3961 tatcggcagt ttgatctatt tttttgttgg tttgcttttt atcaaaaaaa cgacaagacg
     4021 gaaaagacct tatgaaaaaa atcgtcggct tgtcctttac acaagtaaac cgaagacttc
     4081 ttcttttcct tcaggacata cagcgctagc ctttttatca gtagctgcgt tgttcgaatt
     4141 aagtccagtt gcaggaatat tagccttttt atttgcttgt ttgatcgctt attcgaggat
     4201 ttttttactt ttacattatc ctactgacat tttaggagga gctattttag gaagtgttat
     4261 tggcatccta tgcgctgccg ttatctgaat taattgtacg aataaagcat gagtgcgtaa
     4321 atgcgcattt gtgctttatt tttttgtttc gaggaaagcg tagttgttta ttcatttgtc
     4381 ttaataactt aagttttgtt aaatgagaac gttataaaat ttta
//
LOCUS       DAYYME010000173         4355 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000173 DAYYME010000000
VERSION     DAYYME010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.175"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..909
                     /locus_tag="ACHBFW_002637"
     CDS             28..909
                     /locus_tag="ACHBFW_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9318897.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            922..2532
                     /locus_tag="ACHBFW_002638"
     CDS             922..2532
                     /locus_tag="ACHBFW_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9318898.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2609..3268)
                     /locus_tag="ACHBFW_002639"
     CDS             complement(2609..3268)
                     /locus_tag="ACHBFW_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM9318899.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3551..4216)
                     /locus_tag="ACHBFW_002640"
     CDS             complement(3551..4216)
                     /locus_tag="ACHBFW_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFM9318900.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         1164 a          853 c          867 g         1471 t
ORIGIN      
        1 atttttagaa aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac acatttgaaa
       61 aagagctttg gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca ttcaggtgaa
      121 gtcgttggat ttatcggacc aaatggttct ggaaaatcca cgacgattcg gacactttta
      181 gggattataa aaaaagacgg cggaacggca acgatttttg ggcaagatgt atgggaagat
      241 agtttatcta tccatgaacg tctttcttat gttccaggcg atatctcttt gtggggaaat
      301 ttgactggag gagcgatcat cgatctcttc atgaagcttc atggaaaagg gagtgctcaa
      361 aaaaaagatg aactgattca acgtttctct cttgatccta agaaaaaagc aaaaaactat
      421 tcaaaaggaa atcgccaaaa agtgggcttg attgctgctt tggctgttga ttcggatctc
      481 tatttatttg atgagcctac ttctggttta gatcctttga tggaaacgat tttccaagaa
      541 gaagttgaaa aattgaaaga acagggcaaa tccatcctac tttcttctca tattctcagt
      601 gaagtcgagc gactagcgga taaagtcgtg atcatacgcg aaggaacact cgatgaagaa
      661 ggaacactcg atgaattgcg tcatttgact cgttcgacca tcactataga aacagaagca
      721 gagaatagtc acttagcaca attgaacggt gtccatgact ttgtgcaaaa aggtcacctc
      781 gctcaatttg ctgctgataa cgaagctttc gatgtgattt tagctgaagc gtccaaatta
      841 ggtgtgaaaa aatttgaatc tattccaccg actcttgagg atttattcat gagacattat
      901 gaaggatgag gaggaagcga aatgactgat aaattttcac gctggtccac ccttttttct
      961 caaaacctta gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt cactgctttt
     1021 tctggtggtt ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt agctggatta
     1081 tacgaaacct tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc agcttcgaat
     1141 gctgctgagt atactttagg agcgatgtat gcaaatgaaa tgttgctttt ttgcagtttg
     1201 tttgctgctg tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa agaagaactt
     1261 ggtctgattg aacttattcg ttcctttcga gtcggacgtc aagcaaactc tttagctgtc
     1321 atcgctgaaa gtattctgat caatttgctt ttagcaatat cgatcagctg tacgatgatt
     1381 tttttccaag cggattcgat cactgcagaa ggtgcttggc tttttgggct atctgtaggt
     1441 agtatgggga ttttggggag tgccgttgca ctgtttgctg cacaactgat gccaagttca
     1501 gccagttcga atggtctgtc tttagcgttt ttaggtggac tttaccttct acgagcaata
     1561 acagatgtag ctgattcctc cctttcgatg tggaatcctt taggctggtc ttatttgacc
     1621 tatccattta caactaacaa ctggattcct ttgctttatt tgtttttgtt cagtctaatc
     1681 ttgataatca gttcatttgt tttagaggaa catcgagatt tgaacaccgg ctatatcccc
     1741 gaacgtgaag gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg tttattattc
     1801 tatctaaata aagggatgac aatcagttgg cttatcactt ttggtttgat gggggcggca
     1861 tacggctcta tctacgggaa tatgcaacag tttttagaaa gtaacgactt aatgaaacag
     1921 atgtttatcc aatctggcgt ctcaattgaa aaaaacttta caagcacgat catggtagtc
     1981 ttgatttgtc tagctgctat tttaccaatt gctagcatca atcgtttatt tacagaagaa
     2041 acccacggac gtatgaatca gctgttcgca acaaaaattt ctaggaacca actttattgg
     2101 accaatatgt tactagctat tctatttgct atattggcaa tcctgattag ttctggtgca
     2161 ctagggcttt ctgctttggc ttcgatgaag gatgcgaaag acatggatct attgatgttt
     2221 cttaaagccg ggttcaattt cctgcctgcc gttttagtgt ttatcggaat tagtggcttt
     2281 ctattcggat ggcttcctcg aatcggtaaa cttgcttata tctacttagc ttactccttt
     2341 gcactcaatt actttggcgg aattttggat ttaccagaat ggttttccaa aacagctgtc
     2401 cctagctggg ttccacggat gccaaacgat tcttttgatc tttccgtgtt ccttacgtta
     2461 tcatgtatca gccttgcact gttcgtcctt ggttctattg gttataggcg aagagactta
     2521 ctagaaggat aataaaaaag catgtacaat ttcatgctag cctccaaatc tgggtcaaaa
     2581 atccaacatt tggaggtcat ttttttattt acaagtcttc taactgctct ttatcaatga
     2641 tccagacttc tcgattcttt ctttttacat aaccattctc ctctaataat ttaaattttc
     2701 tagataaagt ttcgggggtc gtaccgataa atgcagcaag ttctttcatt ttcattggca
     2761 ggaaaaagtg atccttccct gtcgctttag ctaagtcaag cagatagaga gcgactcgtt
     2821 cttctacttt ttccatcatc aaaaaagttg tctgctgctg tacttttgtt aatttctcaa
     2881 cgttgattgc taaaagtttc agactaaggg cgggataatg cagtaagagt tccgagaaat
     2941 ctttttgtct caatatgcaa atttccgttt tttctagtgc ttcgccaaat aaagactcat
     3001 ttggaacgcc tagcaactgg ctctctccct catatccgcc cggctctatt actcgtaaaa
     3061 gctgttcctt tccgctagca gataattgat acactttcat actgccggag gccacgatca
     3121 ctaattgagg ttctccattc ggcgcaagaa tctgttctcc tttttccaca tagtgatgtt
     3181 tgacaagtgc gtgtactttt tcttgatctt gctgtactaa gtgattgaac aaaggaacaa
     3241 atgaaacaca taaatgatca tgactcataa tctctcctcc ttctaaacag ctatttctaa
     3301 taaagaaaca taacttccct tattccaact gataattttc ttttatcata gcataaacgt
     3361 caagcgaacc agataccagc aaatagagtg gatttctttt tgtcccttgc tttattttcc
     3421 ttcttttgag accttaagta attgattcct atcaattttt taaatattta ctttttgaat
     3481 aatcaaaaac aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat
     3541 ttcgtgttgt ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt
     3601 gtaaggtttc agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt
     3661 tacccatttg aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat
     3721 cggacaaata gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata
     3781 tgtaataatc cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt
     3841 tatacgctgc aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga
     3901 tttggtattg tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt
     3961 cgcttcggta cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga
     4021 ttttaggttt gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct
     4081 ctttccattt ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg
     4141 gatggctctc tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg
     4201 attttccaag ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt
     4261 gcgatatgga caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc
     4321 aaacaaaaaa ctgcgatgag catcaccgca gtttt
//
LOCUS       DAYYME010000174         4352 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000174 DAYYME010000000
VERSION     DAYYME010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.176"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..589
                     /locus_tag="ACHBFW_002641"
     CDS             <1..589
                     /locus_tag="ACHBFW_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318901.1"
                     /translation="KLIEITFPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFV
                     YTYTKTTSQNISKTTLVMEEEDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANV
                     DTEDIRKIIEYLSENTQYLIAECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKS
                     QFLENVEPIDQFKKAFEIKELPEITMHNWLKGEES"
     gene            586..804
                     /locus_tag="ACHBFW_002642"
     CDS             586..804
                     /locus_tag="ACHBFW_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318902.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            791..3418
                     /locus_tag="ACHBFW_002643"
     CDS             791..3418
                     /locus_tag="ACHBFW_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFM9318903.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            3433..3651
                     /locus_tag="ACHBFW_002644"
     CDS             3433..3651
                     /locus_tag="ACHBFW_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318904.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
BASE COUNT         1716 a          559 c          850 g         1227 t
ORIGIN      
        1 taaattgata gaaattactt ttcctacttt ggattgcgaa aaaaaggagt atatgccgaa
       61 aattcgtcag aggggtatcg aaaaaaagag atatgtctct ttaaaaaaag gtagaggtat
      121 gtacaaattt gtatatacgt atacaaagac tacttcacag aacatatcaa agactactct
      181 cgttatggaa gaagaggata ttaatataaa gaggatttac agtaagatta gtgttcttta
      241 tgacgcaaat aacgaagatg gtcgaaaatt atttcaagtg tatcactatt tgagaaacgc
      301 taatgtagat acagaagata tcagaaaaat cattgaatat ttatctgaaa atactcagta
      361 tcttattgct gagtgtatta taaaacaaca tgaagcatgc ttttttcaaa gaaaaactga
      421 agaagggtta tacgattatg taacatattt tttgaatgga cttcagaaaa gatataaaag
      481 tcaattttta gaaaatgtag agcctataga tcagttcaaa aaagctttcg aaatcaaaga
      541 gttacctgaa attactatgc ataattggtt gaaaggagaa gaatcatgat taaaaatcgc
      601 ttaatagatc aaatgtggtg tcttgatcaa aatacagata ctctcgaatt aatcgagagt
      661 atctgcttca acacagtagt tctcgattta agagctgaaa atgacaattg tgtaactcat
      721 atcataatta atcaaaaaat ggctcagcag ctctctgaga gcctaagaaa atgggctgaa
      781 ggagaaaact atgaaagcaa ttaaagaaaa aatcgttagt agaaaaataa actggttagc
      841 actcagtgta cttgttttaa tgattagcgt tgtttttaca agtaatattg ttgaagctag
      901 cattttcaat tggtttgatg acacaaaaag tcaaacagag tttttgaata attctcttta
      961 tagaccttac ctaaaaaaat acaatggaga tttccttagt cagttcggga tttggcttgg
     1021 atgggctgtg gtaaaaggca tgtttactgt gactgattcg attcaaaata tgattccaga
     1081 tgtgttggat ttgttcaatt tcattgaaag tacagggcta aataatgttt atcaatcagt
     1141 cataaataca attgtcgttg gattaatgat cctttcattg atgtttgttg gctacaagat
     1201 gattactgga aaaggaacaa tagatttaag atctgttgga atgaatatag tgatgtctgt
     1261 agcgttaatt cttttaatgc caacaatgat ttctagcggt attcaatttg ccaaaatatt
     1321 ttacaatgat gctactacga ttacaaattc agatgacgga gtcgcttggt cattgataaa
     1381 acaaggagta acagatctgg catatattaa taaaacagat caatactcaa gcattgataa
     1441 aacggaggat cgaaataagt taacacgaaa aaattttcaa cagacagatt taacacaagt
     1501 gttgacggat aaagtgattg ataaattgga aaaagaaaat ccagctgcgg ataatcttcg
     1561 ctatgaatta gttgaaaatt ctaataatga atttgttgcc acaaagtttt ctgataactt
     1621 cttatcaaca ttttctgata gcttgaaatc aggttattat cgatatcaag caaatctttg
     1681 ggggatttcg attgggttga cagcgttggc aatcgcctat gtgttcagcg cgtttgtgat
     1741 cattacagca atattagaac ttgcatttaa acgtgtgtta ggtgttcttg tttttgcgac
     1801 agatattgaa acaggacaac gttcaaaagt tgttttatcc gatattttac aatgttactt
     1861 aacagttgga tttcaagggt ttggactatc tatgtttgct atgtttatca attttttaaa
     1921 tgctggacaa ggtatctcca caaatatttt tataaaaaca attgcttata tctgtgctgt
     1981 gtttgtgctg ataaaaggca gtggaacagt catgagatac tttggtgttg atattggact
     2041 caaagaaggc tatgggcaac ttgcttctgc ttttggtatg ggcgcaatgc tgtttagaaa
     2101 aggatcaact ggatttaatc gtgctaaagg aagcggaaat ggaaatggat cagacagtgg
     2161 agaaggagaa gatcgaaaac cagaaaagaa tttcggcgaa actctatcaa aaaaagctgg
     2221 caaaactgga agagctttgg gatatgccca tgaacgtgga ttatcaggtt tagcttcaga
     2281 tggagctaca atggctagtg aaagagcaat aaaaccattt aagagtatgc gtgatatggc
     2341 taatgatacc aagaataagt tcaaagaagg tttagatgat ggtacagtgt ccgctattaa
     2401 taaaaatagc aaacctatgt tagctaaaaa taaagaagat gaaactggaa aatatgctga
     2461 ttccatgcca acacgtcttt cggatagaaa agatggtgct aaggttgaaa atgccgatag
     2521 aattatgtct agttctgaac gtatgcgtga agcaatgaaa aataatgctg aatcgaataa
     2581 taatcctgta agtgcaattc agcaaaaagt tcaacaagat attgaagaac gtaaaaatgc
     2641 tatgcacggt caagctaagt ctgctgaaga attaattaat cagaagcgac aagaagctaa
     2701 atacacgcct gaagcaatga atcgtgagga aatgttaagg aaacgtgtag aagggcgtac
     2761 aggagctaat acggacgaaa aagaagcgtt gaccaaagaa agaatccaag aagctaaaaa
     2821 ctcaaatacc ggattagaaa aattggtcaa agaaaatcta caaaattcta caagtagtca
     2881 aggtggccga tcagtagatg tccgagaaaa tattcaaggt tcattgaatg gtcatgtcgg
     2941 acggaatgtt gatgtaagag agaatttaca aagctcttca aatggtcaag gtactaagac
     3001 aattgatgtt cgagaaaatg tacaaaattc tacaagtagt caagggttta aatcaatgga
     3061 tgtaagagag aatgtacaaa attctacaag tagtcaaggg tttaaatcaa tggatgtaag
     3121 agagaatgta caaagttcta caagtagtca aggattcaaa acagtagatg ttcgggagaa
     3181 cgttcaaaaa gatagtggag aaatgaaaga aaaaacacaa aaaatcaata tcgttgaaga
     3241 cagaaagtca gcgaaaaaat ttgattcaaa tcatgaaaca gtgattattg atagtgaaat
     3301 ccgtgaaaat gataagggat caaaaccaag acgaagattt acctatgaag acaacgagtt
     3361 attcagagat acacttaatg atcctaatcc gcttttcgat aatctattaa aaaaataaaa
     3421 gggggaattg tcatgaaatt aattctaaag attagtacgc atgatttctt agaacttgac
     3481 gaattatgtg aatcagtaaa gaacgcaaca aatgaaaaaa tcacggtcac taattatatt
     3541 gaactgatga ttcaagaaga attaaagatg aaacatgaga taaaaaacaa cttgaaatat
     3601 tgttatctag aaaaaagtga agagataatc catgaattaa atggctttta aaaattgtat
     3661 ttgacatgaa tttacgacaa ggaattagtg gggattaagc gattattctc ttccacttta
     3721 gagaaataat gggttaaaaa gccagaatac gtagagcaat attgacaacc tataaaggag
     3781 ctattgttga aaattgaagt aacaaaaaga taaagggagg tttacatcta tcagattaaa
     3841 agtgatagta cacccatgaa acctgcaaaa gagttgaacc taatgtatat cggtttaact
     3901 ctttttattt gcaaattaaa aaaatatcac cgaagtgacg aatgaaacta tgaccaaatt
     3961 aagcaagaaa atatatcttg caaataaagt agtataaaaa gatattgata aagccaatca
     4021 aaactttgaa tttcaaacgt tagataatca aacagataca aaaaatgaaa tcagttctac
     4081 gcgtacgatt attaaaaata ataaattttc actagaagaa aatggaaata aaatcaaata
     4141 attaggaaga aaaaacaaag caattagaaa agcctgcaag caaatttgag taatcgagat
     4201 ccaatttttt taaggaagtt taacaaaaat atgtaattct attatcacta ttaaagagaa
     4261 agttggagcg gaatataaag aaaagagaaa agtaattaaa cgcaaaaaaa tgtagaactt
     4321 aaaaaatata aagaggaaca accgttaaaa tt
//
LOCUS       DAYYME010000175         4344 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000175 DAYYME010000000
VERSION     DAYYME010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.177"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..913)
                     /locus_tag="ACHBFW_002645"
     CDS             complement(17..913)
                     /locus_tag="ACHBFW_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9318905.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(906..1586)
                     /locus_tag="ACHBFW_002646"
     CDS             complement(906..1586)
                     /locus_tag="ACHBFW_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9318906.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1587..2369)
                     /locus_tag="ACHBFW_002647"
     CDS             complement(1587..2369)
                     /locus_tag="ACHBFW_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM9318907.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2362..3279)
                     /locus_tag="ACHBFW_002648"
     CDS             complement(2362..3279)
                     /locus_tag="ACHBFW_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9318908.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3443..3697)
                     /locus_tag="ACHBFW_002649"
     CDS             complement(3443..3697)
                     /locus_tag="ACHBFW_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318909.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT         1276 a          947 c          759 g         1362 t
ORIGIN      
        1 cactaatctt gtataattaa accctccgca acttgacgga gatggagaag atgcccccgt
       61 ctaatctagc ggtcatctct cctcccattc cttccaccaa tttttttgac aggtaaagcc
      121 cgattccgct agattccgta tttgtgcggc tggtattttc ggtataaaaa cgctggaaga
      181 ctttttcaac agaggtttgc gttcctggtt tgataggatt ggccactgtt aatcgaatga
      241 atggatcctc ttttgaaaat gtcacctttg cttgtccatc tgcataacgc aataaattct
      301 gtatgatatt ttgcagcact cgcagcaaca tgagcggatc tgctaataca gttgtattta
      361 tcgagtgttc agcgaacgct acctcgatat ttttctcaga aaattgttca taaaaagaga
      421 aaaatacttc ttccacctct ttagaaaaag aaagcgtatg gagatccatt tctttttgct
      481 tcgtctctat tcgagcaaga tcataaaact gatccgtcat actgagtaat cttgcaacag
      541 attgtttgat gatcgttaaa tacttctccc gtttctccgt atcctctacc tgttccagta
      601 attgtacata accgttaata gcagtcaaag gagttctgag atcatgagaa aggccgacga
      661 tcgatagtct gatgttttct tccatttctt gaaatgcttg tcctttttgt tcattttcat
      721 ccaaccatgt attcagtgca tctactaggt ccattagatt cttttctcga aattcgactg
      781 aaagacgacc accatattct tgtcgttcag aaagagtggc gattttctta gttaatctct
      841 tgatttccct ttgcaggaag aaaataaaac ttcccataaa gataacaacg agaagcaaca
      901 gccatttaat caaatctaaa ccccactccc cacaccgtat gaatatacac agcatgtgcc
      961 tttgctagct tccctcgaag attggaaata tgcatattca ctgttttctc actatcaaaa
     1021 tatggttctt gccaaactgt ttcataaaga tttgctcgcg aaaatacttt tttcggatgt
     1081 tctaaaaaaa gcttcagcag atcgaattct cttcctgtca aatggacagc cacaccattc
     1141 acatttacat ccctcgactc tttgtccagt aagatttcct tgtaatagat gcgttgcgac
     1201 agatctttta cttggacaga agtctgatga cgaagttgga tgccgattcg taacaaaagt
     1261 tcttcgatat caaatggttt cgtcacatag tcatccgctc ctgctgccaa caaggaaact
     1321 ttatctcttt gatcgctttt tgctgaaaga acgatgatcg gaacattcga tgtctgtcgg
     1381 atttctgcta acaatgcttc acctgaaagc cctggaagca tgagatctag aaggatcaaa
     1441 tcgacaggat atgtgcttaa caataatttt ccttccgtac ctgaataagc atcaagaagt
     1501 ttgtatctct gttcaagaac ctcctccagt aaggaatgaa cagcttcatc gtcttcgata
     1561 atcaaaatag tttccagcag gaccacctac ttaatgtcag atttttggaa aataaacatt
     1621 cctagagctg atgataccac gattgttact agactgacca gaatcatttt cggtgcttgc
     1681 gaataagttg cttgttgcgc aaagtccgta tacatgagcc acgtttccgt aaaccactcc
     1741 gttagaaatt ccaagccttt gatttttgcg aaaacaccgg gaatgaaggc cgttcccatg
     1801 atataaagaa aagaaagaag caaactgaca ctggtacttc tagtgatgaa tgtcaaagcc
     1861 actggaacag ataatgtccc taatgtcagc agaagcaata caggaaatac ttttagaaca
     1921 taatttccat agtttttgat tccctcaact tcaccaaatc cgaaagcaag tgtgtaagca
     1981 agcatggcta gtcccgtgat caaaaccacg ccaagtgttg tgatcaaaag cgtaacgaga
     2041 agtttcgaca cataataagt cattcgattc cgtcctaaag ataacgtatt gcgaatcgta
     2101 ccgttttgaa attcttcccc aatataaaag cacgcaaatg ctgcgaaagc cagaagaatc
     2161 catgcagcca ttttttctac cattaaaatc cctgttgcac cgttgatgac tgtatccgtt
     2221 gcttggttta attcattgcc gtattttgta tgcatatacc attcgttcca agaataaaaa
     2281 agcggaagta cgaccatcag tcctagtgag acccacattt tacggctctt ccatactttt
     2341 attttttcag cagttaatag attaaacatg tggattgcct ccagttaatt gtaagaaata
     2401 atcttccagt ttttgcccaa ctaggtgaat accatccaca ggaacttttg cttctaccaa
     2461 tgcttgattg atggctgcta cttggtcaat tccttcaaaa agctggataa cacggctaga
     2521 ttgtacaaaa taatccctat aaccaagaga gtcgagaacc gttactgctt gttccgtcgc
     2581 cgaagtgtca atttttataa attgacgaga ttcttgtgcc aattcttctt tcgataattc
     2641 tttgatcaag tgtccttcat gcaaaatacc atagtgagtg gcgatctgtg acaattcatc
     2701 tagcaagtgg ctagaaagca agacggtgat gccccgttct tttaccaaac gttggatgat
     2761 ttctcgcatt tctgccatcc ctgctggatc catcccattc gtcggttcat ccaaaatcaa
     2821 aaattctgga ttcgtgatca gtgcatttgc aatagctaaa cgctggcgca tacctaaaga
     2881 gaaattcttt gctttctttt tccctgtatt ttccaatcgc atcattttca gcaggtcttc
     2941 gatttctcta tccgacacac cgccgttcgc tgcttggata cgtaggttat ctcttgcagt
     3001 taattctgga taaagagcag gggtttcaat cgattgaccc atacgtcgtc ttgctgcctg
     3061 caaatccgtc gttccaaata attcgatcga accttcatca atcgagatca aacccataat
     3121 agttctcata aaggtagact tacccgctcc attttcccca atcaatccat agatcattcc
     3181 acgtttgatt tcgattgata ctttatctaa tgctttggca gcaccgtatt ttttggttat
     3241 accagttgct ttcaagaccg tttcatttcc aaaatccata cttatccttc tttcttattg
     3301 atgagtgaag tatagttgac gggtataaag aaagcagcca gaaatgtata aagaaagtgt
     3361 aaattttcca attgaatgtc aaaaaaaaca atcggacaat acgtgtcttt cctattatca
     3421 ggcagtttgt tatacgcttg tatcagtttc ctgattcttt cttttcgacc aaagtcgttt
     3481 ctctgtattc ctcttcactg aaaatttttg cttctttcag atcaagcttg agtaatttta
     3541 ccagaccggt tataaggata actatagcaa gactccctcc cagaaaaatt agactgtctc
     3601 gcagacgtgg ttgcgttgtc ttccaatcaa agagcgcacc aaacagtgct aatccaagtg
     3661 gactggccac catggtaatc gttgttatcg ttgacataag tcgtccttgt atatgcgttg
     3721 gaacttctcg ctgaagccag accatataaa aagcaaaaga aaacattaat ccttaaaaac
     3781 ggacttatga aaaataatac atgccgtttg ggatttacaa ttagctacca aataagaggt
     3841 tatgacaaaa gacttttcac aatcccttct tccgaataaa cggtgttctc ctgccaactc
     3901 ctcggtcaca agtcacaata tttatcaaac atgagaagca atttttgtaa attccttctt
     3961 atttcttgaa gctgaccact tctgtcacaa cctcagaagc ggataaataa tattgtttga
     4021 tgatacttta tctttttccc gtttgtttat agatcgaatg atattataga ccaagaatcc
     4081 tacctattaa catagctaaa aacacaaaaa aggcactttc caaattgtgg aaagtgccag
     4141 aaaagttagg attaaggtaa ctgcgctcag cattgcttcg cccagtacga ttctcgtaac
     4201 tcgtgaactc gaacgataat cgcaattaac gttttgagaa ctgtgaagct ttacgggctt
     4261 tcttaagacc tggttttttc ttcgtatcat tgaataagtt tgaaaaacct tgatttataa
     4321 aggttttccc gtaaatatat ttta
//
LOCUS       DAYYME010000176         4339 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000176 DAYYME010000000
VERSION     DAYYME010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.178"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      <1..146
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            294..851
                     /locus_tag="ACHBFW_002650"
                     /pseudo
     CDS             294..851
                     /locus_tag="ACHBFW_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            909..2948
                     /locus_tag="ACHBFW_002651"
     CDS             909..2948
                     /locus_tag="ACHBFW_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM9318910.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGEKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKDDDDSATKRASELLQYLNQNYQ"
     gene            complement(3057..4208)
                     /locus_tag="ACHBFW_002652"
     CDS             complement(3057..4208)
                     /locus_tag="ACHBFW_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM9318911.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVFLLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
BASE COUNT         1568 a          802 c          852 g         1117 t
ORIGIN      
        1 ctcctataca aacataggct gttgcccaga aacgtcgaaa gtcgccaatg ggtaaaacag
       61 aaactgttgg attaaggctt tttccaatag ctaagaatgt tttctttacg ttgtatagtg
      121 ctaaaactcg gacgatgaga tgatgctagg tcagaattct tgttcgaaaa ggtttctgac
      181 cttttcttta tatttttgtg actgacttat ttgatgagac aaactcgctc ctcacctgac
      241 aagtggattg ctgtgtgagg aggagatgcg gtgaaaaaag atcgattggt ggaggtcgct
      301 cggattcact tctggttgca caatacaacc agaaaacaca acaagcaaaa ctgatctcta
      361 tcatgagaga ttcatatgtc gatattccag gttacggaat gaataagata aatgcagctt
      421 actcttatgg gggagttgat ttattaaatc aaacattaaa ggaaaatttc aaatttgaag
      481 ctccctatta tgcaagtatc acatttcaag attttatcga ttgcgttaat gaactgtttc
      541 ctggtggagt caagatcgat gcagaaaaat ctctagattt agatggcgta tatataaaca
      601 aaggagagca agtgatggat ggtaatactt tactccagta tgctcgattt cgggaagaca
      661 aagaaggaga cttcgggagg atcagaaggc agcagcaagt gatcaaagct atctctcagc
      721 agctgaaaga cgtaacttca atattcaagc tacccaaagc ggttgggaaa ttacttggta
      781 gtatccaaac gaaccttcca gaaagtgtgc ttctggattg tggtatggat ttcctcaaag
      841 acatatgtta gaataaacag gtataaatag tgaaataaag gaatgacaag caagagaagg
      901 aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct tatattgccg
      961 gcgcagtgat cttaatagca gctgcgggtg gcggttattt ttactaccag cactatcaag
     1021 aaacccaagc agtagaagct ggagaaaaga cagttgagca atttgtccaa gctttgaaca
     1081 agggagatta taacaaagct gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat
     1141 tatctgaaaa agagatctta gatcaatacc agaatatata cggtgctgct gatgtcaaag
     1201 gacttcagat atcgaatcta aaagtagata aaaaagacga ttctacttac agtttttcat
     1261 ataaagcaaa gatgaatacc tcattaggtg aattgaaaga tctttcttat aaaggaacat
     1321 tagacagaaa tgatggtcaa accacgatca actggcaacc caacttggtt tttccagaaa
     1381 tggaaggaaa tgacaaagta agcctgacca cacaagaagc agcaagaggg aatattatag
     1441 atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa acaattagga gtcgttccaa
     1501 gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa agcgattgct tcctccttcg
     1561 acttaacaga agatgctatc aatcaggcga tttcacaaag ctgggtacaa cccgattact
     1621 ttgtcccatt gaaaatcatt gatggagcaa cgccagaact tccagctgga gctaccattc
     1681 aagaagtaga cggcagatat tatcctttgg gtgaagcagc tgctcagctg attggttacg
     1741 tgggagatat cacagcagaa gatattgata agaatccaga attaagcagt aatggaaaaa
     1801 tcggtcgctc aggtttggag atggcttttg ataaggatct tcgtgggaca acaggtgaga
     1861 aattaagcat tacagatgca gacggtgtag agaaaaaagt tctgatcgaa catgaagttc
     1921 aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc acaaaaaaca gcttttgaca
     1981 gtctaggagg aaaagctgga tcaactgttg cgacaacgcc aaaaaccggt gatcttcttg
     2041 cgcttgctag ctctccaagc tatgatccaa acaaaatgac aaacgggatc tcacaagaag
     2101 attacaaagc ttatgaagaa aatcctgaac atccattcat cagccgattt gcgacaggtt
     2161 atgctcctgg atctacgttt aaaataatta cagcagcaat cggtctcgac aacggcacta
     2221 tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg gcaaaaagac agttcttggg
     2281 gatcttttaa aacaacgcgt gtcagtagtg atgtatcaca agtagactta aaaactgctt
     2341 tgatctattc cgataatata tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat
     2401 ttcgtacagg cttagataaa ttcatttttg gtgaagacct tgatttgcca attagtatga
     2461 atccagcaca aatttctaat gaagatagct ttaactcaga tatcttgcta gctgatactg
     2521 gatatggaca gggcgaactt ctaattactc ctatccagca agcagcaatg tattctgttt
     2581 ttaccaacaa tggcacactt gtctatccta aattgattgc agataaagag acaaaagata
     2641 agaagaatgt catcagcgaa acagcagtac aaacgatcgt gccagatctg agagaagttg
     2701 tgcaagatgt aaatggtaca gcacattctc tttctgcttt agggattccg ttggcagcga
     2761 aaactggtac agcggaaatc aaagaaaaac aggatgtaaa ggggcaagag aacagtttct
     2821 tgtttgcttt caatcttgat aaccaaggtt atatgatggt cagcatgttg gaaaataaag
     2881 atgatgatga ttcagcaacc aaacgagcat cggaactatt gcaatacctc aaccaaaatt
     2941 atcaataaaa aattgctttt taagtacagt aaaaggactg tggcgttatc gtcacagtcc
     3001 ttttttcaaa taaacggttt tcacgatgtc tctttcacaa cttctttttg atttactcac
     3061 acttcataaa ctttttttgt aaaatatttg agaacaagtg gagaaataag tgtacttaat
     3121 aaaatcacga taactaaagg tgaatagtac tgtggttcga tcaattgact ttgctgtcca
     3181 atttgtaaaa tgatcaaagc catttctcca cgcgaaatca taccagcacc gaccatcaat
     3241 gaactatgtt gagaaaaacc ggcaatctga gcaccaaaat aacctccaag caatttggtc
     3301 aaaatcgcta caagcgtcaa tatagaaata aagagtagtt gttcactaaa gtcagaaaaa
     3361 tcaacttcta atcctacact gacaaaaaat actggtataa atactgcata tcctaaagcc
     3421 tctacattgt ggtatacttc ttctttaacc tttgtctgac tgacagctat cccagcaaaa
     3481 aaagcaccaa tgacagagct aagcccaaca agatccgcta cgtaagacat tcctaaacaa
     3541 ataactagcg atacgatgat aatagaagaa tttgcgtaca ctttttctgc aaatgaaagt
     3601 aggaaaggcg caatccattt gactaataag aaaataaaaa caaaataaat tagctgttca
     3661 atcaaaacaa gcggtaaaga agcattgcta gaagcttccc ccgttagaaa agataggctg
     3721 aaacttagaa ctaaaacgac taaaatgtca tctaccacag aagcacccaa aattgttgat
     3781 ccttcttttg tattcactac gtttagttct tttaatactt ctactgaaat actgacagaa
     3841 gttgccgcaa gaataatccc aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg
     3901 cctgtcaacc agccaaaaac aaccgggaag atgatcccaa gcaaagcaac gaacatacca
     3961 ggtcggaaat attttttcaa aagagataaa tcactttcca gaccagctaa aaacattaat
     4021 aagaacacac caatctccga aaagtcatgg actagaatat caggtttgac ccaattcaat
     4081 ccagcttggc ctagcaaaac accaacaagt aattgtccaa tcactgctgg gatacctaat
     4141 ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata tcaggcataa aataccaata
     4201 aattccattc tcatcattcc tttgtataaa atatagcccg aacttagcat aacatacatt
     4261 attgaaaaaa taggaaaata tactcacaaa aaaaccaaca atccaaaaga tttttttgga
     4321 ttgttggttt ttatacggt
//
LOCUS       DAYYME010000177         4277 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000177 DAYYME010000000
VERSION     DAYYME010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.179"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            239..991
                     /locus_tag="ACHBFW_002653"
     CDS             239..991
                     /locus_tag="ACHBFW_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9318912.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(1131..2054)
                     /locus_tag="ACHBFW_002654"
     CDS             complement(1131..2054)
                     /locus_tag="ACHBFW_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9318913.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(2069..2341)
                     /locus_tag="ACHBFW_002655"
     CDS             complement(2069..2341)
                     /locus_tag="ACHBFW_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318914.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(2685..3872)
                     /locus_tag="ACHBFW_002656"
     CDS             complement(2685..3872)
                     /locus_tag="ACHBFW_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFM9318915.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            complement(3979..4191)
                     /locus_tag="ACHBFW_002657"
     CDS             complement(3979..4191)
                     /locus_tag="ACHBFW_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein"
                     /protein_id="HFM9318916.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIN"
BASE COUNT         1346 a          845 c          707 g         1379 t
ORIGIN      
        1 tttcgattag cttcatctca ttcctctttt ctatatagtc gatttagatc caatcttaag
       61 ttatgttacc atctttccta gcattaagga atcggttaca attgaacctt taaagacaaa
      121 aggatttacc ttttatcact taatagtata acataatttc gtgagaaaaa aatacaattt
      181 tttcttgacc atctcgttac gtaaaggatt atgcttacat ttgtcaggag gaaaaataat
      241 ggagtacaca ataaagaaaa tgtcagagat atcaggagtc agtccgagaa ccttgcgctt
      301 ttatgatgag attggactgc taaaaccagc taggatcaat tcttcaggtt atcggattta
      361 tggcaaaaaa gaagtcgatc gtctgcaaca catcttattt tatcgaacaa tggcattcaa
      421 gttggatgat atccaagagg tcttagacaa tccatctttt gaccaccaga aagctttaat
      481 caagcatcag caaatgttgc tggaaaaacg ggcacaaatc gataccctct tgacaactgt
      541 ccaacagaca cttgatatgt acgaaggagg aagaaaaatg agtgacaaag aaaaatttga
      601 agggtttaaa caacaaaagt tgaaagaaaa tgaagaatcc tatggacaag agattcgaca
      661 aaaatacgga aaaaaaaagg ttacagcatc caatgaaaaa tggatgaata tggataaaga
      721 aacgtatgaa gaaatgcaaa ctgttgaaaa gcagatgtta tttgatctca ctctttactt
      781 aaaagaacca gaagatgaaa gcttagcaga ccgtatcttc caagctcata agaagtggtt
      841 ctcttacagc tggcctgaat atcagccaaa agcgcataaa ggactaggaa tgatgtacga
      901 agccgatgaa cggttcactg catattatga cgagcgaagt ggtcatggtg cagcaaaagc
      961 tttaaatgaa attatccaaa aaaaggcgta atcttttaca tgatccaaca aaaaagtaca
     1021 cgtaaaatga cctaataaca aaacagcaga cacccaagtt agatttttat gctaactttt
     1081 ggtgtctgct ccattctttc acaagttttt tcatcctact ttaaatatat tcatcgttga
     1141 ataaacgtat aaggtatttt cactttatcc tttttttctg taagaaacaa ttgaaatgcc
     1201 atttctccgc aagcttccag atggtggtcg atggttggga aaccaagaag ctcgctgatc
     1261 agcaaattat cgcgtccaat caccactggt gtcttatctt gcagatagct ttgcaagatg
     1321 cctgctgcaa ccatatccga atttgtaaag acaccatcaa ctccaagctc tttaaaaaag
     1381 aagcctgctt gttttccttt ctctgcatct atacaatccc aaaaaacaag tgattcatca
     1441 aaagatggaa aaagttgatg acatagctgc aaagtgattt ttgagttata acttagcctg
     1501 ccacttcgcc ccaacgtgat gcccaattgc tttatacctt gctgcttcaa ataagtaaga
     1561 ccttctgtaa ttgattgttc tcgatcaata aagacacagc ttgcctgttt tcccttgatt
     1621 tcttcacaaa aaactagagg tccatatttt tgatatgcca ataaatcatc tagtgtattg
     1681 gcacgagacg taatgatcaa tccatcgaaa gctttagctg caaattcttc aagatacctt
     1741 cgctcttttt ctttatgata gcccgtcggt aaaagtgtca ctttgtaatc ataatcaaac
     1801 gctgcttcaa tgatcccact gatcaactga tcaaaatacg gatgattggt ataaggcaga
     1861 atcactccaa tgcttttcgt ttttccataa cttaactgtc tggcatttgc attagggata
     1921 taatcgactt ctttcatgac cgcaagtatt tctttccttt tttcctcatc tacatatgga
     1981 tgtccattga ttacacgtga aatagtagaa actgaataac ctgtgagctt tgcgatatct
     2041 cgaatgtttg ccactgctgc ttcgccacct atacaaaaag attttcttgt aaatagtcta
     2101 ccacagctgg accgcattca aaaaactgat acatttttcc ttcatgcgaa aaactgatcc
     2161 gctgcccgcc ccaatatata tcttttgtca aattttcgat ttcattcatt ggtatttctt
     2221 ttactatatt cctattcaac ccttgaaaac tgtacaacgt taatacggat cccttgaact
     2281 cgacaaacgc ataaaaataa ttgttcgttg tgccatctgt tatctctaca taagtattca
     2341 ttgcaagccc tccttttgat actttgagta tagtgtatga aacgcgtctc atagcaagga
     2401 gtttgttttg ccaaagaaaa gttttattcc tctttcatta ttaccaaagt ctcttatttt
     2461 aaattgaata cattataatg atactgtaaa tatatcttta ttattagtga atggagtcaa
     2521 gtccacaatc ttgtgtaaaa gttcaacctc atcaaaagca caatgttttg cgttgtgaac
     2581 ctaactgcca tgcctttctt tctgaggaga gaggtcagga gcagtagcgc agcgactctt
     2641 gatgtctctc ctcagaaaga agagactcgc acaggttcat ggtgctaaat gttattcgaa
     2701 ttaaattcta aatattttcg tggagaagcc agccactcag tgtctttttc catcaaaagt
     2761 gttccaatta tccgagtgat ggaattgata tttggaaaaa tccgaatcac tcgttctctc
     2821 cggcgaatct cttcgttcaa ccgttccaac acattggttg tacgaatcct tcggcggata
     2881 ttttcaggaa aagacaaaat ttgaatagca tcttcaaagc cgttttctaa aatgtcgcaa
     2941 gcagcactta attttgaatc acaaccatat ttttccaaga agtgctcttt tctttcacgt
     3001 gtcaattcaa gttcagaggc tttaaagata ctcttcaact cgtcttttac atcagaagaa
     3061 actttctttg gcagcttatc aaaaatattt ctcaaaaagt gggcttggca tctttgccag
     3121 ctcgcaccta aaaagttctt gcgaacagcc ttaactaacc ctttgtgtgc atcagaaatg
     3181 accagtttta caccgaacaa acctcgttga acgaacgatt gaaaaacggt atcccaatta
     3241 tcttccgatt cgccgtcttg aacatcaaaa ccaagaatcg tacgtcgtcc atcagaatca
     3301 atgccgatac caatatatac accttttgag acaatacgat gattttccct tactttcata
     3361 tacagggcat cacacatgaa aaatggatat tccgagcctt ctaggctccg gtttctccac
     3421 tcaaaaacaa atggatccaa ctgtttcatc agatttgaaa cgaatgattt agaaacagtc
     3481 gcaccatccg ttagcagttc aactgtttta gttactttgc gagtggagac accagaaatg
     3541 accatttcaa tcatggcagc gatcaaagct ttttcgcttc gctgatatct ttcaaagata
     3601 gtaggactaa acttaccgtc tctggttcgt ggaacatgaa gttctaacgt accaattcga
     3661 gttgtaaacg aacgatcgta gtaaccattt ctttgtgatt gccgttcttc tgaacgttca
     3721 taggcatttg cctgaatata ttgatttcgt tgttcttcca tcagctgatt aaacatgata
     3781 gtcataagtg tttttgcggt atcattcgca ccgctttcgt ttataattgc ttgaatttct
     3841 tcgctttcaa aagtaaaatg tacttgagtc attgatatca gtcctattcg agtatgtttt
     3901 ctgcaaaaaa cattgtactc ctaactgaga tcaatggctt ttttgatgct tcacttttac
     3961 acaattatat ggacgtaatc aattaatatc attttttaac atagtaattt gtttatttaa
     4021 atctgatagt gtttcttcct tacttaatat aaatctttct ctatcatgta atttatttag
     4081 tttttcatca tttttttgtt ctaattcata agcttctttt tttattgtgt tagctttcga
     4141 cagtttagtg attcctataa ttaataaaat aagtccaatc aataataaca ttcaaaactc
     4201 ctcatttctt tgatataatt attgtagagt ttactcagaa atgaggaaag agtccatgtg
     4261 aaagcatggg ctttttt
//
LOCUS       DAYYME010000178         4267 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000178 DAYYME010000000
VERSION     DAYYME010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.180"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..1383
                     /locus_tag="ACHBFW_002658"
     CDS             82..1383
                     /locus_tag="ACHBFW_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9318917.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1410..1670
                     /locus_tag="ACHBFW_002659"
     CDS             1410..1670
                     /locus_tag="ACHBFW_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM9318918.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2669..3904)
                     /locus_tag="ACHBFW_002660"
     CDS             complement(2669..3904)
                     /locus_tag="ACHBFW_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9318919.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1237 a          682 c          881 g         1467 t
ORIGIN      
        1 taattatata attagttatg aacattcttt gcgttgtcat gcaatgaatg gttcatattt
       61 tattgaaaag gagtggtttt agtggaaaag ctgatgaaat gggtcgaaga gaaacttgtc
      121 ccaccaatgg caaaaattgg tacacagcgt catttattag caattcgtaa tggggttgtt
      181 tcaacattgt cattgatttt gattgggaca ttttttatgg tctttatcaa tttaccgttt
      241 cctggatgga atgaatttat tgccccttat tcggcaacga ttgttttacc ctttaggatg
      301 acgatgggat tgatggctat ttatgcaact tttgttatgg gttcggatct agctaaatca
      361 tatggattag atagtgtgac aggtggtata ttatctttag gaacgttctt tatgttacaa
      421 gttcctgtaa acgttttaac tcctgaagag gcacctcttg gctgggtctt acctatgagt
      481 tctttgggag catctgggat gtttgcgggg atcttatcta tgatttttgc agtggaagta
      541 tatcgtttct ttaaaaatcg aaatattact atcaaaatgc cagaacaagt gccacctgct
      601 gttgcccgta gttttgaggc attgattcca ggtgcagtca ttttaacaat tacttggctg
      661 attcgaagcg tcgcgggctt tgatgtaaac gctgccttat tatcgttatt cgagccttta
      721 accaatattt tagggaataa tttgcttggt gttttactac caatgttttt gatccatttg
      781 ttgtggtcat ttggtatcca tggcatgagt attgtagggg ctgttgttcg tccaatgtgg
      841 ctgatcatgt tggatgagaa tgcaaaggca ttggcagatg gtacagctgc cacccaatta
      901 ccctatatcg ctcctgaaca gttttatcaa tggacggtaa caattggtgg tgcaggagca
      961 acgatcgtgg tatctgtttt gtttatattc ttctgtaaat caaaattctt gaaagaggtt
     1021 ggtcgttttt ctattattcc tggtatcttc aatatcaatg agcccataat ttttggagct
     1081 cctatgatgt tgaatcctta catgttcatc ccgttcaact tagttccttt agttttgact
     1141 attatcagtt attttgcagt caaattagaa atggtcaata gatttactgt tttgccaaca
     1201 tggacattac cagctccaat cggcggttat ctttcagcag gaaatgactg gcgtgttgtt
     1261 atcttgatca tgatcaacac attgatagca tttattatct attatccatt tgtaaaagct
     1321 tatgataaaa agatgctgtc agatgaacaa agtcgtggag aaaacgaagt ccagactgtc
     1381 taagtttggt ttttagaagg tgggaaacaa tgtattggct gattttattt tttgttttta
     1441 tctttttatt aacggctagt caccttatat tgaatatgct agctacatat catattcaaa
     1501 tcaatcgatg gatttgggca ctggcatcat ttttgatcgt gattcttcca aagatcattg
     1561 taccgcatat gaatgttttg ttttcatggg ggacatatgt actttgtgga atttttgcta
     1621 tcaattttat gatcgagcag catagatggt ttgtcacatc gaagctttaa atgttctaaa
     1681 agtggttgta acagaagtgg ttagcttcaa gaaataagaa gaaatttacg aaaattgctt
     1741 cttaaatttt tgtgaaatgt tggcttattt ttcgtttggt tgaacttatt accgcagatg
     1801 caagcttttg accaccgttt attcggaata tgggactgtg acaaggcttt tgtcacagcc
     1861 cttttttaca taaagattat tattgtttta actgttttgg cagaaagaag gaggaaaaaa
     1921 tggaaatttt atcagcaatg gatttagaat tagtagatct actaccaaag tgatcaagtc
     1981 aaatatagat ttgggagctt acaaccatat agtaggggct gcagtatggt gtatgaatgg
     2041 taatatgtat gatgtgagtg tttattcaaa tcatgaaacg tgtatagaaa taattgtgct
     2101 gggaatagca atcagtcatg gtgaacgtga cttttagtgt atcgttgctg acgacataga
     2161 acattctgac atgatctact caatatgtgg ggattgctgg cagtttatat aggagtatgc
     2221 atcggattgc gagatcattg tctcaacaga agagggaggt tgattatcaa agataaagtg
     2281 gtaaatccta catttgattt agtaattgtt gaggggacta gcggcacaaa tctctacaaa
     2341 gcgatgcttc tagatctata tgacacattt taagaaaatg tatttgccta attattatgg
     2401 tctttctttt gaagaaacgt tgagatgatc caaccagaga aaggtgtcaa agaatttgac
     2461 taagaataaa tggcagacgc tacctatgct catttggata gcgtcagatc ttttatttta
     2521 aggatgattt tgtgtttttt agacaccgta atttaagtaa ctctaacttt tcaatccact
     2581 atttactttt tttgtatttt cactgtctgg gtctcgaata gcgaaattac tgatgagtct
     2641 ctattaaaaa agctttttct acttgaaact atattgggtg gttgctcagg aaggtttcga
     2701 tttcttgaat tgcttgttgt ggaccattta ttggaaaaac ggcttgcagc gtgcgtgcct
     2761 gctgacgaat cttgggtgaa gaaacttcgc gcagagcaat ctgcagtttt ctttcagtca
     2821 ctggttttgt gagggcgatt gcgtttccgt attttacaca atagtcaata ttacagcttt
     2881 gctcaaattg catccccaca ccgataaacg gtttgccgga ttcacaggca gtttggacgg
     2941 tcccttcccc gccgtggata aaagaaaaat caatgaattc atgcaaaaga tgactagaca
     3001 gatattctgt tattaaaaga tcttctgagt cagattgaat gtcatcaagc agatagtcaa
     3061 taggtgcaat aaccgctaga tccggttgtt tttgtaagaa gaacaaaatt ttgcgtaaca
     3121 tacgcggatt gcctgaacta cccattgcga aatagataat acgttttctt tcttgttgct
     3181 ttcgctgtat aaaagttagc acttcttcag gcaaggaagt actgagctta gcaaaaattg
     3241 gaccaacgat tcgataattt tccggcagct gttcttcttc caaaaatagt ttaggcgttg
     3301 tgatgagatt tagatcagca tccatcattt gtaaacttgt ttttggtaaa ggaagcattt
     3361 ctttttgtgc agcgtgtttg aaggcattgg ggatccatcg ggtatttact atacgctttt
     3421 ttgtccagtg ccaaagggga ttgcttaaag ggccgaagaa acgtaactcg tggggcatca
     3481 gcgcatgctc tgattgaaaa aaaggttcac tcaaagcata gggtttaaca tatactaaag
     3541 gaatcttttt attgcgggcc gataaaaaaa ttgtcacatt cgaacctgtc acaatcagtc
     3601 gaggctcaaa cttagcaatc agactctgtt cactagtgat acgagaggca accatttctt
     3661 tggtaaaagg gtttttaagt gtacgcaact ggtcaaattt tatgatttgt tcttcttgtt
     3721 ttttcgtaag gatcggcgct agtaaatgat actcaaagcc actttctgtg atcaaaggag
     3781 caaaagtttt agaaaagcca aagaatacac agtaataacg gtctttcata gctttggcaa
     3841 cctcaatcat tcgtgttgtc tcagctaaat tgaatactgc aggagcaaaa agaattgtct
     3901 tcatgaaatc ctccttattg gaatgagtct atcccttcgt ttcttactag tatactaaaa
     3961 aacacagcgt tcgagtagcc tatgtatatt ttgaatatta aactgtctct cgctgttttc
     4021 acaacttcta aatttaaatt ttgtaatcaa gttgaaactc caattttcaa aaaaacacgc
     4081 aacaattttt tgatcgattt atatgttgtg ccagataaat acgagaagtt gtgtcattaa
     4141 aaataaacat gagaacataa aaaaataaaa taattatatt aattgatagg tgtgctgtcg
     4201 tcattataca cacgtaagaa agaaatagtt atgtttaaaa atattcttag actttctcgc
     4261 catgaca
//
LOCUS       DAYYME010000179         4240 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000179 DAYYME010000000
VERSION     DAYYME010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.181"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..745
                     /locus_tag="ACHBFW_002661"
     CDS             44..745
                     /locus_tag="ACHBFW_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM9318920.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1317..2333)
                     /gene="tsaD"
                     /locus_tag="ACHBFW_002662"
     CDS             complement(1317..2333)
                     /gene="tsaD"
                     /locus_tag="ACHBFW_002662"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFM9318921.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2330..2794)
                     /gene="rimI"
                     /locus_tag="ACHBFW_002663"
     CDS             complement(2330..2794)
                     /gene="rimI"
                     /locus_tag="ACHBFW_002663"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM9318922.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2801..3343)
                     /gene="rimI"
                     /locus_tag="ACHBFW_002664"
     CDS             complement(2801..3343)
                     /gene="rimI"
                     /locus_tag="ACHBFW_002664"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM9318923.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3327..4049)
                     /gene="tsaB"
                     /locus_tag="ACHBFW_002665"
     CDS             complement(3327..4049)
                     /gene="tsaB"
                     /locus_tag="ACHBFW_002665"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFM9318924.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1248 a          911 c          735 g         1346 t
ORIGIN      
        1 aagtgacaat aaatatagtg aacaataagg agaggacttt ttaatgcgtg ttaataaaat
       61 cgattttcac ttttcaaaac tcagatatca acctgatttt gaacatttct cggatgaaga
      121 gttccaacag atcaaggaac atactgttct tcgttcttat aaaaaaggac agatcctttt
      181 cgacgatggt gatcccagac aacggttgta tatcttgacc aaaggactcg ttcgtttaga
      241 acgttatgat gaaagtgcaa cttattacta ctatgactat gtgaatacaa ataacctttt
      301 tcctatgtca ggaatgtttg gagaagagac ctacacttat acagctcaag cgatgactga
      361 tattgaaaca ttctaccttc ctgtttccta ctatgaaaaa ttagcacgaa aaaatcccga
      421 acagttgatc tatatgatac aaaaaatttc taaagtagtt gaaatacatg aattgcgtat
      481 ccaaatcgga ttgacctcta gcgcctttga ccgggtaaaa caaaatttat tcattttgaa
      541 agaagaattg ggcgtaccaa caaaagatgg tcatatcacc attccttatc cgatcacctt
      601 aaaagaatta gcagtgaata gcggcacgac acgtgaaact gctggacaag taatcaaaca
      661 actacgtgat tccggactgt tagattatga caaaaagcgt tttactttct gtaagacagc
      721 tactactcca gctatgagag aataacgcga acaaaaataa gcagtgaccc tgatatttat
      781 cagggtcact gcttattttc ctccctaaaa cttctgaaag atccggcagt tttagatcac
      841 tgggcaagac tcaaagattt tctatgtgtg tttcagttat gtaatattca ctgtttgcaa
      901 aaaatactga tagattaact agaggaagcg tcttgtcctc atccgaccta gattattcga
      961 tattcctcga caataaacga ttaatcagtg agggcaatca gaggaacgga taatgatgaa
     1021 taagctgtaa aaaatagggc tttgacaaaa gcgtgtcaca gcccttattt cgattaaacg
     1081 gtgtgtacct gtcaactctt cggtcacaag tcataatgtt cataaacatg aggagctgtt
     1141 ttttgaaatt gttccttgtg aatcagagtt aaacgacagg cttacgaact cttgattaaa
     1201 cggcgttcaa aagcttgcat acgcggtaat aagaacagcc aggtcaaaaa catgagaagc
     1261 cgtttttcct gggctgttct tattacttga tgcagggcag atttttcaca acctctttat
     1321 tcagataaaa ccaagcttgg atcagcattc aactgatagc tagcaaaatt tcttttttgc
     1381 atttccacat gtgctgctgc tccaatcatt gcagcattat ctccacaaag tcgaagaggg
     1441 ggaatcacta gttctacctc agggagttta gttgccaaag catcctgcaa tccttcacgc
     1501 aacccttgat tggccgctac acctccagct acaatcagtt gcttgactgg ataattttga
     1561 caagctcgaa gtgttttagt aatcaataca tcgatcacac ttgcttgaaa gctagctgct
     1621 aaatcatttt tgtctagatc ttctccacgc tgttgtgcat tatgcaccaa attgataaat
     1681 gcacttttta atccgctaaa actgaaatca taattatctt catggatcat cgctcgtgga
     1741 aaatggtaat tatcttttcc ctgatgagct agctgatcga tttcttttcc gcttgggtaa
     1801 gaaagaccta atactcttcc aactttgtca taagcttctc ctgctgcgtc atctcttgtc
     1861 tccccaatga tttcatatga tccatcttct tgcatataga ctaattctgt atgtcctccg
     1921 ctaacaagaa gtgccatcaa tggaaactgg aatggtttaa ccagacgggc tgcatagata
     1981 tgccctgcca tatggtttac aggaataagc ggcaattggt gtgcccaagc aaaagcctta
     2041 gctgcagaaa taccaatcag caacgagccg acaagtcctg gtccataagt gacagcgact
     2101 gccgataaat cttctgccga cacaccagct tctaccagtg catcttccaa gcataaggtg
     2161 atttgttcta cgtgatggcg gctagctact tcaggaacta cgccaccaaa acgtttatga
     2221 ctgtttattt gagaagcaac gatgttagat aagatctctg ttccatttct aacgaccgct
     2281 acactcgttt catcacaact tgattcaatc gccagaatca attcttcatt catttcatcg
     2341 tctccgtttt taactttctg ctcataatga tcgcatcttc cgtcggattg cgataatatg
     2401 atttccgttt acctaatata cgaaactttt ctgattcata aagttttctt gctgcttcat
     2461 tagatagacg tacttctaaa aaaacatgca gcgaatcttt tatttgttcc gtaatcagta
     2521 attgtaacaa atgccgtgca tgcccttttc cttgttctga aactgcaaca gcaatatttg
     2581 tgatctcgac ttcatctatt actttcgagt atccaataaa gccacagatc tggttatctt
     2641 caactaaaag cagataatca gagtgaggct gttggatgtc tgccaaaaac tgcattttcg
     2701 tccaaggtga accatgtgga taagcatttt cgctaatcat ccatacgcat tctgccaagt
     2761 acgcttcatc aaaatctttt tttctgcaaa tcaactaatc tcactcattt tctaaaagtt
     2821 ttatcatatc caacgcatct tcttttgttt cagtgtaata tctttttttg atcgttgttg
     2881 cttgaaaacc taacttccga taaagtcttt gtgcatcttg attactcata cgcacttcta
     2941 atgacatcgt ctcacagcga ttcattatag caaagttctc gatttcgtca ataagcagcg
     3001 aaccgattcg tttacgctga tatgccggtc gaacggcaat attcgtgata tggcaatcta
     3061 aaccgaacgc tcttgaaccg ataaaaccaa tcattcttcc attatcaaag atacctagat
     3121 atagatgagg aagcggtgac ttcaattctg atataaaggc actttttgtc catggcaatt
     3181 cgccattata gacttctcgt tcgacatcca gcagctcttt gatatcttct gtagtcaatt
     3241 ctcttagtac gtaacgacga tcgatttgtt cgattgtttt ttctgtataa atcattttat
     3301 cttgaaaaaa gcttctggca tacgctctaa attttttcaa cataactttc gtctccaggt
     3361 gtgtgcgtct ccaaccattt ttcttctgct tctacccgtt ttaaatagcg aggaagaaaa
     3421 gcttgtttct ctgcaactgg ttcagctaaa gcacctaatt gagccacagt tacaccactt
     3481 ggaatatccc atgctggaac tgtattaatc atcgcactag gcaatatttc ctttatttca
     3541 tagaagaatt tgtgacaatc actcccgacg aaataaatag cttcattcag cttttgtagt
     3601 tgtgtgaaca aatcagaagc agaaatatgt acatcttcaa gtacaatttc taatctattt
     3661 tctttccagc ggtatgcccc agcgtataca ttttttcttc gagcatcaaa catgggcaca
     3721 ataattttat ccacaccgat gcaattagcc gcgatggttt tcagactaga tactcctact
     3781 agctctttat ctaatgtata agcaatcgtt tttgctgtcg ttacacctat acgaagccct
     3841 gtataagaac cgggaccatc agaaaccgcg attcggtcaa tgtcttttgg acttatttga
     3901 agatctgcaa acaattggtc gattgcaggc atcagtgtta cactgtggtt gcgtttgata
     3961 tttgtttgta tttgtccaag aatttgttcg tcttctacca caccgatcgc caaagtttgg
     4021 tttgcagtat ctattcctaa tgtaatcata tatttgatcc tttctgctat gacattctcg
     4081 ccttccattg tagcatactt ttttattctg aggatgaaaa taccaaaggc aaaactagta
     4141 caatgataca tgattatctg ctggcgggca aattttgccc cttcctgaaa cacaaaaaag
     4201 ctgcggtatc acttcatttc cccacagctt ttccatcttt
//
LOCUS       DAYYME010000180         4220 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000180 DAYYME010000000
VERSION     DAYYME010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.182"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            11..463
                     /locus_tag="ACHBFW_002666"
     CDS             11..463
                     /locus_tag="ACHBFW_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318925.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            477..1166
                     /locus_tag="ACHBFW_002667"
     CDS             477..1166
                     /locus_tag="ACHBFW_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318926.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1291..3642
                     /locus_tag="ACHBFW_002668"
     CDS             1291..3642
                     /locus_tag="ACHBFW_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFM9318927.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3722..4117
                     /locus_tag="ACHBFW_002669"
     CDS             3722..4117
                     /locus_tag="ACHBFW_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318928.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1486 a          531 c          856 g         1347 t
ORIGIN      
        1 ggtgagaatc gtgaaaaaaa cgatactaag tattttttca gtatttacaa tattaattgg
       61 gggctatttt cttttgaatt tagattctgt taattcaaac gctcaaataa aagatcaaag
      121 agtagctgaa cataataata aaaaaatgag tttgaccaaa aatatctatt ctatgtcaga
      181 tgaagaattg tataaagaat ttggtgattt atccggggaa cagttagaag aaagtattcc
      241 aggaattagt gactcagata aaattgttgt cttgtctgct ggtgggcaat taatgggaga
      301 tggcaaatcc gaattagtta gttataaaca aaatcacaga cagtttattg atgttaatac
      361 tgattccaac gctatttcga ttaaggatct aaaagagctt ttaaatagta aaagaaatga
      421 attaaagcag ataaaagaaa ctaattcagt ggaattagag taaaggaaga ttcattatga
      481 aaaagatttt tggtgtttct gttgggctac ttatgatgtt aacatttgga aatgcagtat
      541 atgctgctga gaaagaagtt atttcagatc cagagcagta cgcctcacga tactttgctg
      601 attttgaaga agaagataaa gtacttgcat tgccagaagg tggtttttta aaaggggaag
      661 ctaaaattgt atctatggat aatccagata taattcaagg ttcatataat tcagaaactg
      721 atcctgcttc agtttcagtg acagaagcaa aaaaaattgt tgctgaaaaa ttcaaaaatg
      781 atattgaaac tactccaatt atgcctcgag gagcatttcc acccgatgtt ggtactagcg
      841 gacaccttct tgtactagca ggaggaggaa gctataaatc acagccattt agtggtgcgg
      901 gatggcgttt tggaggtatg ggtgtaaaac cagctgatgg tacaggcaac tttttacgtt
      961 ggaaatccta cattgacgat ggaagagtag gaaatgtaaa tcaagccaac gcaactaaat
     1021 ctggttctat tcaaggaatg gcattgtatg ctcgtgaagg attcaaatac tttgcaccaa
     1081 acgggcctgg ttggacaatg aatataggta tttattacac ttataatcct gttgcaggta
     1141 cgtattatgt tgttcaaaat atttaataaa acagcacaaa gagatttctt tgtgctgttt
     1201 tattattttt agttgttgac aaacactaaa atataaaata taatcaacac ataaacgaac
     1261 gtatgttcgg caagaaagca ggggattttt atggatgaga tggttttaag tactcagaaa
     1321 tggctaaaca agaaatacag taatgttaca gggtttgata aggttccaga aaatgggcga
     1381 acagggtggc ctacaatcta tggacttatt gaaggactac aagtagaact tggtattact
     1441 aatctagtag ctaattttgg acctaccact gaaaaaatgt acgataatca ggttactcct
     1501 aaatggggca aaaatctacc taaaaatatt gtatttctta ttcaaggtgc cttttggtgt
     1561 aaggggatta atccaggtgg cttcgatggg gtatatactc catatttaga tactgctgta
     1621 aaagagttgc agactgatgc agggtttaat ggaagcgctg taactggagt tttggatgcg
     1681 aaatgggcca aagcgttatt tgatatgagt gcttttgttc ttgttcccgg cggagatagt
     1741 aaaattagaa aaatgcagca atggttaaat gtaaattatt tagaatacac gggaataatg
     1801 ccttgtgatg gtatttatca aagaaattca aatcaagcat taatttttgc attacaagct
     1861 gaactaggat attctcccag tgaagcaaca gggtcttatg gtaatgggac aactagtaaa
     1921 acttatcctg tgtctgaagg taactcggga aactatgtaa gaataattca atatggatta
     1981 tatgttaatg gttattttga agacggtaca ttcgatggta ttttcactaa atatatgggt
     2041 ttagaagtac tggcattccg aaaatttatg gtattgcctg aatatactga aatagcagat
     2101 gttgttgtaa taaaaggact attgtctagc gctggagata ttagaagatc tgcagatggc
     2161 gcagatactt ctacgcaatt aactagatct caaatccaaa cattggttga taatgatata
     2221 aaaatagttg gtagatatct tactggtact gttggaattg gaaaagatga acgaaataag
     2281 tatctaacta cagaggaatt gaataatatt ttttctaaaa atttatctgt gtttcctatc
     2341 tatcaagatg gaggtgcagc attagcttat tttacttatg atcgtggctt atcagatggt
     2401 aagaaggcta tagatgcagc aaaaaatcta aatataccat taacaacagt tatttatttt
     2461 gctgttgatc tagacatgtt aggggaagaa attcttgcat atgctggaga atattttaag
     2521 ggggtatctg ctgtaatgtc atatagcggg tatcaaacgg gtgtttacgg aacaagaaat
     2581 gtttgttctc aaataatcaa taacgggtat gcttcgtttg catttgtttc agatatgtcg
     2641 actggttata gtgggaattt aggttttcct atgcctagac aatggtcttt tgatcaattt
     2701 gttgaattta ccataggaag tggcaacgga gctgttggta tcgatttaat cgctacatcg
     2761 ggtagagata gcggatttaa tgagttatca aatgatacta ataatgataa ttacattgcc
     2821 aaatacaatc agaaggttat aaatcagatg gtacgtgcgt atcaatatct atcacaagct
     2881 ggtcttgata atccttggaa ccctcatttg acattttata gatatgtaaa ttattctggt
     2941 ttgtcatggg acatcatttc ttctcctgta acagagcatg atagaaaaat ttatgatgaa
     3001 tataggaata aattaactaa tagcgagggc ctgtataatt attttataga tcctaatact
     3061 ggttcaataa taggtttgcc tcacttaatt gtgacgttac aatcacagat gtttctagcc
     3121 gacacaatca atgatagtgt ttctgacttt gcaggttggt taggagattt aatgacttgc
     3181 tggggtgaag ttaagaaatt aggaatacaa atggagcaag gtgtatttgc attagtaggt
     3241 acggctgcag gtggaacatt tagtctagaa gatttatacc aagatgttga cgcggtaaat
     3301 gtttatacct tagcaaaaag aaattctgat tcaacagctt tatcggcttt aaaaacgtac
     3361 tattgtgata agggtgctat tgatagattt aatcaatttg ttaagaacag atttggttct
     3421 tcagataaaa tcgcttcagt aacaaatagt ttattagaaa atgacgaaac agttgatatc
     3481 ttaggtgtag ggataatttc atttttcatt gataaaatag ttaataattc acttgggaca
     3541 gattttactt atcttgattt tattggagat gaggggaaag ttgcaaatgg attttctgaa
     3601 aaaattattc aatttcaaga aaatagaagt aaacttaatt aagaatcgtt tactatttaa
     3661 acagtgtcct tttccttttt aaatatatac gataaaataa gtatatgaat tgtaaaagga
     3721 catgatgatt atggaagaaa tagaatggaa taatctgact caaaataata aaaataattc
     3781 tggatgtgga attatcttta tgtttgttat agttttggga atagtagtac ttttaataac
     3841 tgtcaagata gtagattact ttgcgaatac tcctgaagaa caaatacaga gaaaacttcc
     3901 tgagtttgaa agagtaccac cttatgatcc aagagataat tcatttcaag taaaaaaatt
     3961 aaaagagtct ccaaatgaaa tgtggtttga agttacagtt gaatcaagcg acttcaaaga
     4021 aaaaaagaaa gaatacaatt atattgtaaa ggcaaaagta tcaaaaagat ttatttttaa
     4081 ccctaaatta gaaatatata gtgttgtaat agaataatag tgttaaaaaa attgtaaaag
     4141 ttaaagaagt caaattctga tgtgaaaaat aaaatatacc atggaagaca taaaagaaaa
     4201 actggataac tagtttattt
//
LOCUS       DAYYME010000181         4216 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000181 DAYYME010000000
VERSION     DAYYME010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.183"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..1564)
                     /locus_tag="ACHBFW_002670"
     CDS             complement(59..1564)
                     /locus_tag="ACHBFW_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9318929.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDPFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRAPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1561..2247)
                     /locus_tag="ACHBFW_002671"
     CDS             complement(1561..2247)
                     /locus_tag="ACHBFW_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9318930.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2440..3861)
                     /gene="gndA"
                     /locus_tag="ACHBFW_002672"
     CDS             complement(2440..3861)
                     /gene="gndA"
                     /locus_tag="ACHBFW_002672"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFM9318931.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1177 a          886 c          729 g         1424 t
ORIGIN      
        1 ttgtattttg taccttattc tttgttggat atataaaaaa acaagtaaag cacatgcttt
       61 acttgtcttc tgcctttgcg ataggaataa agattctaaa aattgtccct tgccctacta
      121 cactttctgc taagatacgt cctttatagt tttcaaccag ttgtttagcg atagacaacc
      181 ccagaccatt tccacccttc gttctagctc ttgctttatc cactcgataa aaacgattga
      241 agatcttatt aaggtcttct ttgggtattc cttcgccaaa gtcctgaatc gcgatctcaa
      301 attcattcag agtggaagaa atagagatat ggacttcttt tcgtgtagtt gaatacttta
      361 ctgcgttatc caatatgata atgattaatt gttcaaagtg attacgataa atttgcaggg
      421 tcacttcttt tgtcaaatca tcatctaaag tgatcacata gtctggatat aatatcttga
      481 aattattata aacttgatag gtgacttctt ttcctttagt tgtttcattc gcataatgaa
      541 catctacttg ttctgctctt gataagtcta gcatctcttg aactaagctt ttcatacggc
      601 tgatttcttg catgctggcg cttaatgatt cttctaatat ctcaggatca tctttccccc
      661 aacgatttag cagactaaga tgcccttcaa taattgcaac aggtgccctt aactcatgag
      721 atacgtcttc cacgaattgc tcttgttgtt caatatacaa acgcatacgg tcaagcatct
      781 cattgaatat ttcagctaaa tctgccaatt catcatttgt gtcgatttca ggcatgtgga
      841 tatctgattg cggatcttta cgtatagtat ccattgtatc tctgagtacc ttgagtggtt
      901 ttaagaaata agaagataaa aagaaaccta aaatactact taagatcaaa gaaacaactt
      961 ctaaaacaat aagagtcaat aatagcttat ttcgaatatc atagaaagaa gataattcat
     1021 agaagctttg cgcatagccg atcttttctc ttgtaccttt agaatatata ggctggatag
     1081 ataaaaagcc agtttttcct tctagtgtaa cgatcgttgg aactttacgt gtagtctgaa
     1141 tcaggtttcg actgttttca tgggttttaa aaatcaagtt ctcgtccaaa tcataaacat
     1201 aaagatccat tgatggctgc cctagctcag ctataaaggg atcgatcttc attaagtttc
     1261 cttcgaccag tgaagtatga tctacgacct catctccgtc atttgtcgct ttcgtcagat
     1321 tacgatacgt atttactaat gtcagttctt catccgagtt agccaaacga gaagctactt
     1381 ctgaaatcgt acgctctaca ttgtttcttt cttttgccac aatcagattg atagatgatt
     1441 tataggtaat gacagcgaaa attgtaaaca caacgaatat gaagaaagaa ctcgcaaagg
     1501 cccacttgat tgtcaaggag ggccctttca gttctctttt ttccgatttt ttcggcttta
     1561 tcacgagcgc atcacataac ctgttccgcg tacagtttgg atatagcttt cctccccggg
     1621 aacgtcaatt ttgttgcgaa gataacgaat atatacgtcc actacgtttg tttcaacttc
     1681 tgtttcgtat ccccacactt tatttagtaa aacgtcacgt gctaatacca cgttcacgtt
     1741 ttccattaaa gtaagcagca actcgtattc tctttttgtt agttcaatta cttcatttcc
     1801 gcgacgtacg acacggtttt ctttttcgat agtcaaatcg cgataagtaa tagtagtttg
     1861 tttagccaca tttttatcgc cttcaatatc gattcgacgc aacaatgcac gaagacgtgc
     1921 taataattct tcgatagcaa atggtttgac gatgtagtca tctgctccgt ggtcaagtcc
     1981 agatacacga tcgatcacag aatctcttgc agtcatcata atgattggtg tatttttgac
     2041 ttggcgtaca cgtctgcaga cttctaaacc gttcaattct gggagcatca gatccaacaa
     2101 tatggcatcc cactcattat ttaatgccgc atctaaacca gttcggccgt tgtagtggac
     2161 ttctgcgtta tacccttcat gcttcaactc tagctcgaca aatcgtgcca agttcttttc
     2221 atcttcaatg attagaatat tgctcattta ttattttatc ctttctattc tactggcttt
     2281 ttaaaaactc tatcattata ccgataatat gcttaaaaat ctatagtcat tttatcatgg
     2341 atagcaattt taaaaaagag ctgaagtcta ctcataacaa tcgacttcag ctctttcgtc
     2401 ctctttttat tttctcattt cctaacagcc gaaagactat tattcttcat ggtaccatga
     2461 gtaatggaag attccttctt tgtcgacacg ctcataggta tgtgcgccaa agtaatcgcg
     2521 ttgggcttgg attatatttg caggcagacg ttctgctctg tatgaatcga agtaagcgat
     2581 ggctgatgag aaagtcggta caggtacgcc agcttggaca gccaaggcaa cgacgtctct
     2641 gactgattgc tgatattttt tcgtgatttc aacgaagtaa tcatctagca ataaattttc
     2701 gatctcagga tttttgtcat aggcatccgt aattttttgc aagaatcgag ctcggatgat
     2761 acatccagcg cgccagattt ttgcgatttc accaaatggc aagtcccaat caaattcttt
     2821 tgaagcgaca cgtaattgag caaatccttg ggcataactc atgattttac tgaaatacaa
     2881 cgcttctcgg attttttcga ccaattcttt tttgtctccc gtgaactcaa aatcgttcgt
     2941 acgagacaaa atcttgcttg cttgtacgcg ttcttcttta taagcagaaa tatagcgggc
     3001 aaatacagat tcagtgatca atggaagtgg cacacctaag tcaagcgcgc tttggcttgt
     3061 ccatttacct gttcctttat tcccagctgc atcaaggatc acatctacta ctggtttacc
     3121 tgttccttca tcgtctttac gtgtcagaat gtcagcagtg atttctatca aatagctgtc
     3181 taattcgcct tgattccatt ctttaaagat atcagccatt tcttctactg aaaggtttaa
     3241 aatattcttc attaagtcat aagattcagc aatcagttgc atatctccat actcaatacc
     3301 gttgtgtacc attttcacat aatgtccggc tccatttggg ccaatatatg tgacacatgg
     3361 tgctccgtct tctgcttttg cagaaatttg ctctaagatt ggtgcaacta gcgcataggc
     3421 ttctttttgt ccaccaggca tgattgaagg tccttttaaa gctccttctt caccaccgga
     3481 aacgcctgta ccgataaagt tgatgccaga gttggctaac tcttcattac gacgcatcgt
     3541 atctttaaag aatgtatttc ctccgtcaat caaaatatcg cctttatcaa ggtgaggaag
     3601 taactcttgg attgttgcat ctgttgccgg tcctgctttg accattagca agatccgacg
     3661 tggtttctcg atagattcta caaattcttc gattgtatat gttgctttga aatttttatc
     3721 tgggtgttct tctactacag cggttgtttt agcccctgta cgattgaata aagccactga
     3781 atagccgcgg ctttctatgt tcaaggctag atttttaccc ataacggcca taccgacaac
     3841 gccgatttgt tgttttgaca tatgaactcc tcctacatac gggatttctc cctaatctct
     3901 tacattatag cttaacacgg ctacattgca aactaaagaa caacatattt tggctctata
     3961 atttatggga ctgatgaatc agaattgatt cttcagcccc atttttattt taggtattaa
     4021 aaaacatatc gttctctagt atgattaaat cacgacaaaa taaacaactg agaggacgat
     4081 atgctatatg aatgattcta tcaaaaaaat gctgcgatta atagagaaag atttgatgat
     4141 tacagaggtc tcttacgaga cctttcagaa gaaaaagacg ttgatcgtcg atgctgtttt
     4201 ctcacctgct ccccat
//
LOCUS       DAYYME010000182         4206 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000182 DAYYME010000000
VERSION     DAYYME010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.184"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..361
                     /locus_tag="ACHBFW_002673"
     CDS             116..361
                     /locus_tag="ACHBFW_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318932.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            459..680
                     /locus_tag="ACHBFW_002674"
     CDS             459..680
                     /locus_tag="ACHBFW_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318933.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            1002..1682
                     /locus_tag="ACHBFW_002675"
                     /pseudo
     CDS             1002..1682
                     /locus_tag="ACHBFW_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            1822..2361
                     /locus_tag="ACHBFW_002676"
     CDS             1822..2361
                     /locus_tag="ACHBFW_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318934.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            3381..>4206
                     /locus_tag="ACHBFW_002677"
     CDS             3381..>4206
                     /locus_tag="ACHBFW_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM9318935.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKL"
BASE COUNT         1539 a          581 c          742 g         1344 t
ORIGIN      
        1 ttctaaaagt taaatagttt ataatagaag aaaaagaatg gggtgacgat ttcttagatt
       61 gaaaaggaaa gataaatatt atagattatt acttttgaca gctgaagcag gatggatgga
      121 ttttccagaa gttgtaaaag aagttcaaga gataggtgca actatttctg atagccgctc
      181 ctctcctatg aactatcagg atgcccatgg aattaaaata attgatcaag agagaaatgt
      241 cagaaaatac acaacaataa atgagtgtgt aattaatgaa aatctttgta gggcaccgat
      301 aacgaaacac attaagaata gaagcaaagc aaaagacgga agaactttta ctaccttcta
      361 agtttttttc atatttgttt gctatactta gatctaagga attagacttt cgctgcccat
      421 tatttaagtt tgaggtagcg tcttgcgagg gatcaataat ggaagaagaa aaaatatttg
      481 aaaaacgctg ggaattggct tcagttgaac aaagagcaag gtaccataat ctaatgtcct
      541 cttaccgtaa tattgattgg acttacaaag aaaagaagta cttgctttgg ttatgtcagt
      601 tagatgtaaa tacctttgaa acatttgaag tgattctaga taagattaaa aacagcaatg
      661 aaaaaagagc tgatctataa tggttcgctc tttttatttt attaactctt tggttactat
      721 atgtaaaata cagcttcata ttacaataaa tttcaggata caaatttatt taagcatata
      781 taaatataat tgaaatagga cataaatata tgagtatgag aaaggtgatt ccatgtttaa
      841 taaatgggca aaccacccaa tgaactcgag aggtagagat aatttgaagg agacggacaa
      901 gttctgggaa gaagtatata gtaaaaacgg ttctgttgca aagttttcca aaaaatctat
      961 tttagtgtaa aattgagaaa aaagacagag aggacagagt aatgaatcat tttaaaggca
     1021 aacaattcaa aaaagacgtc attattgtcg ctgttggtta ctacctgcgt tacaatctaa
     1081 gctatcgtga agttcaggaa ttgttatatg atcgtggaat aaatgtttgt catactacga
     1141 tttatcgttg ggtataagag tacagcaaag tcctctatta tctttggaag aagaaaaata
     1201 gacaatcctt ctattcatgg aaaatggacg aaacctatat caaaattaag ggacgttggc
     1261 attatcttta tcgtgcaatt gatgcggacg gcttaacctt agatatctgg ttacgaaaga
     1321 aacgggatac gcaagcagcc tatgctttct taaaacgact ccataaacag tttggtgagc
     1381 cgaaagcaat tgtgaccgat aaagcacctt ctcttggctc cgcctttaga aagttacaga
     1441 gtgtgggttt atatactaag acagagcacc gaactgtgaa gtatcttaac aatttaatag
     1501 aacaagacca tcgacctatt aaacgacgaa ataaatttta tcaaagtctc cgtacagcct
     1561 cttccacgat taagggcatg gagaccattc gaggaatata taaaaagaac cgaagaaatg
     1621 gaacgctctt cggcttttcg gtgtctactg aaatcaaggt attaatggga ataacagcct
     1681 aagatatttg gagttcagag agggcgcgtt tgattttcaa acttcgcaac agaaccgaga
     1741 accttgggtt ttcaagtgct ttttggttca ttaatcttaa ccatagtgtt tttaaagtgg
     1801 aaaacaagca acaaaaaaag tatgtggagc cttgtaggaa tagttcttat tatttttact
     1861 gtgactgcag caacagaagc aaattattcg cataaaattc agtcgtataa aggtcgttta
     1921 gttatgacca aaaaagatgt tacacgttat acatcctcaa gaactggagg cctatatgac
     1981 tatgcaccat tagatgcatt gaaatataaa gatcatttaa agaaacatga agtaaaagtt
     2041 ggtgatcggt gggagaatgc atctcataaa gcatataatt ccaaaatagt ctatacagta
     2101 gaagatgccg atgagcaaaa tatcacatta cctgtattca attattgggg aacagttgtg
     2161 aaagtaaatg ggaaaaagag cactatagaa aatgacaatg gtttagtgtc tattaaaggt
     2221 acggggaaaa gaactatagt tgaggtttca tctacgtatc caactacgat gattctatct
     2281 ttattaatta gttcaataac ttttatattc ttatttacac gttatttaat aagatgctgc
     2341 ggtgaaatga aaatagatta gatagatatt ataaatatgt tgattctatt ttcatagcaa
     2401 tggagaaaat acattagcaa tatataaaag aaggtatcaa gagtcacagt gaatttattc
     2461 cgaaaagtga aaagagcaaa tataaaattt aatgaaaata tagaactaaa taggaaaacg
     2521 tagacgtaat tttctacgtt ttccttttca tatatattaa atattacgga aaattatata
     2581 aactaattta gtgttttgtt tgtaattcct aattttgctc cacttttaga taatttttag
     2641 gtctatttaa ctcatcagac attctatgct catttttctc caaatttgtt tcaactcttt
     2701 cgtacttaat tttgcgtatt tgaaaattct tcttttagag tacttttatt acgatactag
     2761 tatgatatat atctatagca aacagttaaa atcttcctaa aataagcgaa tagcattcct
     2821 aaaacctata aaatttttag atgaagcgac aagtggacaa ttaataaact acttgaacat
     2881 gaaattatgg atcacctttc aaatttagaa aatggaattc ttatacttat tgcgcaacat
     2941 ttattgattg caaaattatg tgacaaaaaa aactagtaga agaaagaata atggcttgat
     3001 aataaagtac tacctattaa aggtagtact ttatttaatt tagtataaat taacatacta
     3061 ctatatttta ggtttcatat tatataaatt aataatatac taccatttgt tggtagtata
     3121 atttaaaggt aataagttat tcttttatta ctatgtttgg gttttatagc aaagaaagtt
     3181 taataagagt ttggacctag caaacaatat taatgaggtc attaaatctt attatcttta
     3241 gtgatataac gaaatatcta cacagacaaa atgatagcgt aaaatatttt gtgtaaatag
     3301 atatttaggg tagaaaaaaa gaaagtccat tctgtaaaat taaagttacc acaaccgaaa
     3361 ttttatagga ggactttctc atgcccaatt ttactacaga aattatggaa acactaatca
     3421 ataaaggtga tttagatgaa ttatttcgat gtcatttaga attagctgtt aattcattat
     3481 tgcaggctga attaacagct tttcttgatt atgaaaaata tgatcgtgct gggttcaatt
     3541 caggtaattc ccgtaatggg aattattcac gttcattcag aacagagtat ggagaattaa
     3601 atttggtaat tcctagagat agaaatggta agttttctca acaaacttta cctgcttata
     3661 aaagaaccaa tgactcttta gaaaccacta ttattcagct atttcaaaaa ggaattacta
     3721 tgtctgaaat ctctgaattg attgaaaaaa tgtacgggca tcactatacg ccacaaacca
     3781 tttcaaatat gactaaaata gtttctgaag atattattgc ctttaaagaa aggtccttag
     3841 aatccagata ctctgtcatt tttatggatg ctactcacat tcctttaaaa agacaaactg
     3901 tttcaaaaga agcagtttat attgtaattg gcattcgatt agatggaacc aaagaggttc
     3961 ttgggtttag tcttgctcca actgaatctg cttatgtttg gaaagagatt cttcaagacc
     4021 taaaagaccg tggtttaaaa gaggttttat tagtcgtaac tgatggttta agtggcatta
     4081 acgatagtat tcatagtgtt tatccaaatg ctcaatttca acaatgttgt gtccatatat
     4141 ctagaaatat cgctcataag gttcgtgtta gtgatcgaca agaaatctgt agcgatttca
     4201 aattag
//
LOCUS       DAYYME010000183         4189 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000183 DAYYME010000000
VERSION     DAYYME010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.185"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(343..846)
                     /locus_tag="ACHBFW_002678"
     CDS             complement(343..846)
                     /locus_tag="ACHBFW_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM9318936.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1205..2230
                     /locus_tag="ACHBFW_002679"
     CDS             1205..2230
                     /locus_tag="ACHBFW_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM9318937.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2553..3431
                     /locus_tag="ACHBFW_002680"
                     /pseudo
     CDS             2553..3431
                     /locus_tag="ACHBFW_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
     gene            3907..4023
                     /locus_tag="ACHBFW_002681"
     CDS             3907..4023
                     /locus_tag="ACHBFW_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM9318938.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         1383 a          662 c          780 g         1364 t
ORIGIN      
        1 aggagaaaaa ccatagaaaa tataaagtaa ttacttataa aacaagcttt ttatagaaaa
       61 ataatatacc tgagtagggt ataaattaag acggaattcc tttctagatt gagatattga
      121 ctaggaagaa tgttaagatt ttaagccttt aagctaaaaa aaaacccttt ttgttaaata
      181 aggggaatat agcctgtcag tttcaaactg caagtcgtcc tgctgcaata atatagtaga
      241 ttagagatga aaatagattt ttttaaacca atgactgaat aaaaagaccg tcatagatac
      301 acattggaat gtgtctatta cggtcttttt ttacgaacga ttttattgaa gacggacagt
      361 tcgggtcaaa aataaaaaag ctacgatcag aaataagaat aaaatgtatc ccaaaataat
      421 ggctagacgg tctaacttat ttgtcgatag atgtttgttt agaagttgat aggcccataa
      481 acctatcagt cctcctgttg tattagttat caaatcagtg atgtccgcta cgccaatacc
      541 gaataggaat tgaatcaatt ctgccaagaa gcttacaagg aaaataatcc ctaatttttt
      601 agaaaaagaa agtctcttga aattgacatt caaaagcacc ccaaaaggaa aaaagacaat
      661 caagttataa gtggtctcac gtaataccgt cgtattttca aaagaaaaag tagaaaaagg
      721 gacgagattg acattgctgt aagaataagt gagaaattgt ggaagattaa atgatagctt
      781 gaataaaatc aaccataata gaaagaataa ataaatccct aataaaaatt ttgatacata
      841 ccgcatagac atctcctcca tttaaaaagt ataccatcga aaaccgctga aaaatcataa
      901 aaaagtatga agtttaagga ccaacgtagt tatcgtacga aaattagaag cttttttttg
      961 ttgggaatcc ggtttcttta acaaagctaa gatagcgagt tgcttggaaa agttgctatc
     1021 ttttttctgt caaaacgact cggaatgttc gttttttgtt ctaatttctc caagttgttt
     1081 actttttgat actaagttta tgaaaaatca agaaatagtt gttatactag gtaatgctga
     1141 caaacgggat tggcatttca tcttggttca gctttttttc caattactag gaaggaggga
     1201 tagattgttc aagacttatt ttcgtttcaa taaaatacaa gataatcctt ttcgtatcat
     1261 gaatctgatg ctacttatct ttattgtatt atggttaggt tacataaatc atgatttttt
     1321 gatttcaagt ttttcatcat tagggatctt tacttttttc tattatcaaa acataccgat
     1381 gaaatatttg ctgaaacgta tgtttctgat cggttgcgga ctgttggttg ctttcgtttt
     1441 ggggatctta tcgacttatg ttttatggct tgaaccattt gccgtagctt tggttgcttt
     1501 ttccagcaga ttcgtgttgc gattattcca tatttctaaa ccaggtggct tgttttttgc
     1561 gatgctttct gccatgggaa cgagcatgca gctgccaatc gctcaattac cgatcgtctc
     1621 actttatttt tttatgggtg tcgtttttgc cctgattgcg gcagtgatta caaaattact
     1681 ggattcacgg cctgagcaga caattgaaaa agcaacttta aaagaaagat ttcacgaaga
     1741 gcctttagtg atcattgatt ctgttttcta cagcgctgcc ttatttcttt ccgtttatgt
     1801 cagtcacggc ttgaatctgc ataatcctta ttggttgacc ttgtcatgtg catcgatttt
     1861 gctagcagaa aacttggatg cgatgaagca tcgacaagtc caatatctga ttggatcgat
     1921 gggtgggtta tgtgtgtcgg cattcttgtc atttgtgccg tttacgcagt tgcagaccat
     1981 ttttctgatc acttttctat atggaatcgc acaatttcta gttgctagaa attatgcagt
     2041 tgctaatatt tttcttaatc cgatggcgtt gatgctatct acacttattc gtggagcata
     2101 cttgatttct ttgatcgaat atcgtttttt agggatcgtc ataggaagtt tcatcggttt
     2161 aggtgttgcg tgggtaatga cggttggttt gcaacattat ttaacggttg ttcgtcaaaa
     2221 attagaatag aaggacaaat atatgtgcca aaagaggtta tgacaagatt tttgtcacaa
     2281 cctctaattc aaggtgttca aaagccagat tctcagcatt aagtcaaatt cacacaaaaa
     2341 tgtggaaagg aactttcaaa aaaattttat atttattcta tcaaaaaatt ttgtttatga
     2401 tggtaactga ataaactttc ctgagatggt caatgctttc cttgaaagtc ttacagatat
     2461 tctttcttca tagaaagttt aagttttata gttagtgtac aataaaagta tgtttatacc
     2521 atatggtgtc cggataatca gtcatttaaa caatgaggtt caaagatatg aaaaggggaa
     2581 atttgatgaa gaaaataagc aatgaaatcc aaattctggc agaaacttta actcttccca
     2641 aagtcgttat agagcaaatg attgtacaag caaaaaacat ccttgatgag gtgtttgagc
     2701 aatatagtca tgaactaatg gatcctaata aagcaaaggc gactgcaaag aaaatagaca
     2761 aagtctatga agaggaacct tttgcagcat tagctattta tctatatgca gcttctcaaa
     2821 gttggaaaaa aatttatcaa ccattgaaga ttccaagaac tatctatttg gcaacaatga
     2881 atgcttttac tagatttatt catgaacatt tccaagtaac aggaagctat cgctttgacc
     2941 gtggtttttg gatttggcat tacactagtg gcttgatctt tcggattggt gaattggagt
     3001 acgaaatgac atattttccg aaagggtaca cggatctaag attagaagga aagcagtgcc
     3061 tttctcttca tattccttcc gatgcagatt tatctcagaa taagataaga gatagttacg
     3121 aaagtgccat tcgttttttt gaactttatt ttcctgatta ccattaccaa gtgatgtaca
     3181 cagatacttg gttactatca cccaatctta ctaaatagtt gaaaaaagag tcaaaaatct
     3241 gtctttttgc agccgattat cgattattat cagttgatga acaggacgat agtggagtcc
     3301 cttggatctt tggacgagta gatgcccaaa tacatgatta tccagaaagt acgagtttac
     3361 agcgacaagc aaaagagcaa ttgcttgcag gcgagcacat tggctctggc ttaggtattc
     3421 ttacttcata agtttagtac ttcgaaatag tttaaaagag cgtgagacaa aagtaaagaa
     3481 cacttttgtc tcacgctcta aacacgtata aacggcggaa gcagaagcaa ctccttcgga
     3541 aataagctga aattcacaaa aatttgaaaa gcaattttcg tgaatttctt cttatttctt
     3601 ggagttaaac gcttctgtcc cagcctcttt tttagtgttc tatcgaacac acagtacgtt
     3661 caataggtaa actagctata acaatagata ttaaagttaa cggacaagag ggcctttttt
     3721 aggtagctaa aacgtaataa tgaaaattta tttccagaga taggagtcaa tcagctagtc
     3781 tgatcgactg ttttaacaag ttgactttca ttttaacttt tgataccatt taaaacacaa
     3841 atcgtaattg tttcgatttg tgttttttag catgactaca atcaaagaag aaagtgggtc
     3901 gtccatatgg aaaaaacaga tatctcaagt gcataccgcc gcttgaaaag cccaaatatc
     3961 aaaacacgaa aacgtgcgct gaagatcatc aaagatgtga aacggaatag tgggaaaagg
     4021 taagaagaag gaaacttaat taaaaaaata gccgaaaaac gtttttcggc tattttcaat
     4081 aatcggtttt tgtgtaaggt ttccgaaaga actgctgttg attttccatt tttaacatat
     4141 gataaattgg tagaatcttt cttttcaaat tgataccatt ccctaattc
//
LOCUS       DAYYME010000184         4179 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000184 DAYYME010000000
VERSION     DAYYME010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.186"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..526
                     /locus_tag="ACHBFW_002682"
     CDS             <1..526
                     /locus_tag="ACHBFW_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318939.1"
                     /translation="FKEYLEIINFRLKTKKESVIILVYFQGGNLMIKKLFLLSILTLA
                     MAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCSNRR
                     RCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAFSFA
                     REDEDNPKTYDILA"
     gene            complement(587..952)
                     /locus_tag="ACHBFW_002683"
     CDS             complement(587..952)
                     /locus_tag="ACHBFW_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFM9318940.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1028..1273)
                     /locus_tag="ACHBFW_002684"
     CDS             complement(1028..1273)
                     /locus_tag="ACHBFW_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9318941.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1343..1597)
                     /locus_tag="ACHBFW_002685"
     CDS             complement(1343..1597)
                     /locus_tag="ACHBFW_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318942.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1761..2084)
                     /locus_tag="ACHBFW_002686"
     CDS             complement(1761..2084)
                     /locus_tag="ACHBFW_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318943.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2271..2344
                     /locus_tag="ACHBFW_002687"
     tRNA            2271..2344
                     /locus_tag="ACHBFW_002687"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2305..2307,aa:Ile,seq:cat)
     gene            complement(2729..3226)
                     /locus_tag="ACHBFW_002688"
     CDS             complement(2729..3226)
                     /locus_tag="ACHBFW_002688"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFM9318944.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3266..4075)
                     /locus_tag="ACHBFW_002689"
     CDS             complement(3266..4075)
                     /locus_tag="ACHBFW_002689"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9318945.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT         1403 a          730 c          730 g         1316 t
ORIGIN      
        1 ttttaaagaa tatttagaaa ttattaattt tcgtctgaaa acaaaaaaag aaagcgttat
       61 tattttagtc tattttcagg gggggaacct tatgataaaa aaactatttt tactatcaat
      121 attaacgcta gcaatggcag gttgttctga ctctggctta gcaactgaac tgatcacaaa
      181 atcaactgaa gccacgacag aggagttaaa atgtggaaac gatcacagtg actacagagg
      241 cttaaatcga cgatgcatgg gaatattagg cagaagcaat tgtaagcatc gatgtagtaa
      301 tcgaagaagg tgtagaagaa gcaacagaaa aatcaaaaga aagatcaaaa gaagcaatct
      361 gtgctgtgca tcgtatacag acgtcgaaga tgcggtactt ttaaaccacg tcaggagatg
      421 ttcatttatg tctagggaag ttggcgatga accaaaacta gagtacgctt ttagttttgc
      481 acgtgaggat gaagataacc ctaaaactta cgacatttta gcttgatcta agatggttaa
      541 agctttttaa tgaagtaaga ggagatagaa ggcttttcta gttatattag gtaagataaa
      601 ttcctttttg ctccaaattt gctaaacatt ctgctagata aacttcatcg tgttccggat
      661 aaatcggcac gttgcgctct acagtaggta aatcaaaaaa tgcttcgcat gatttgccac
      721 gatacatagg atttttccac tctggtgctg ggctgtaacc acgtttttcc atttcatcca
      781 tgacaagctg atgatattgg aataatctat aaggagaata gtgaaaaaca taatccacag
      841 tagcatgttt ctttccccag ccatttcctc ttaatgcaca gcattcccga tgttgtccta
      901 aaagctgttg acgaggaagc tttgaaatca atttttcatg ccataatcgc atagaaatgt
      961 ctcctttata tatagattgt taaccttaga cgttaaatga aaaaaccagc accaaggctg
     1021 gaaaaaatca cttcagtttg cttgctgttt ctgagcgacc aattaggtaa tcaacggaaa
     1081 tatcgtaaaa gtcagcgata tagattaatt caatgatgct tggaatttct tctccttctt
     1141 cgtacttacg atatttgaat aaaggaatac ttaacgcgct agaaacagct acttgagata
     1201 aagatcggcc agttcttaag aaattcagac gacaacgtaa agtatccagt aaaatagctt
     1261 cttcgatact catttaatca cctttcatta tagtagaata gttataatga ctatagaaag
     1321 ctacacatgc aatcaatagt actcaatgtt ttttatttaa acgtatcaaa taaagaatga
     1381 cgatcaataa agacaaaacc agtcctgata attcttctat aaaaacacta tcgggttcaa
     1441 agtagttgta attaaaaact ttccacataa agtagatagc cggaagagtc aaaatagcta
     1501 aataaaaccg ttctttgttc caattcataa gataaacaat acttgcgacc agtgcgaccg
     1561 caggcaccca aaacaaaact ctcaccaacg taatcatgta acggactcct caacgattca
     1621 tataataaaa gataacatgt tattgcatac atcacaaatg aaatgctagg aaaataaaag
     1681 aagaatagat caatgaaaaa tgaaaaagca gaacagtttt ctgctgataa gcaggaactg
     1741 ttccgctgaa tgtgtcttat ctatcgagaa aaaatcaatg gattgatatc taagtaattc
     1801 aatacatata aaaagaggac gatcgcattc actaaaaagg aaagaatgat gatcccatag
     1861 cgaatggaaa catatttaac aaatactttt gtttgaatac gggcatatct ttttccttta
     1921 tagctgagca tcatgacaag aagcaaaagg gtaatcacac cttgaaaaat cattccaata
     1981 aacagaattc ctgaggtttg ataatttaat tggagaaaat aagctgctgc cgcaactaaa
     2041 tcgaataaaa tcgcaataaa tggcaaaata atcactccta acattgagaa taaataaaag
     2101 aataacattt tttctaaaat aagcaaataa aaattaaagt aacaaaatag ttatgacaca
     2161 agtaaaagaa ataagaataa caatattatt ttacttatat tttgaaactt gtgagagttt
     2221 gtcattcgtg ctatagttga aaagtaacaa aaataaattt tatttatgat gtacctttag
     2281 ctcagttggt tagagcagac ggctcataac cgtccggtcg taggttcgag tcctacaagg
     2341 tacataagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc caagtaaaaa
     2401 cagcttctta tgtttttact tggctgttct tattactgca gatgcaagct tttgaatacc
     2461 gtttaaggaa gaggttgtga aatcagcgtt ttgacctgag tattagctta acaaacggaa
     2521 aaatagttcc tcatattttg ccgtttgtta agctaatacc gagggtcagc tgattgaaca
     2581 ccatttaaga aagaggtcgt gagcctgccg tttcactctg tgtcataaaa aagccaatct
     2641 actttgatag aaaaagcacg taggactaaa acagttctgc gtgctttttt tattgtgtgt
     2701 taaatatcgt aaatgaagat agatattttt atgattcgac gatagagacg ttgaaatctt
     2761 tcaacagctc taaagcggat tctccgtgtt ttctactgga gactaacatt ggatcaacaa
     2821 aaaagtctgc ttcaggaaaa gcactctgca ggcctatcag attagagagt acacacaagt
     2881 ttgtttcaat cccgactact tcgatctctt tttgttcttc tttgctttca aataaagttt
     2941 cctggatttc agctaaagtt tcaggtggaa tcgtaaagta atacttttta atcactcgtt
     3001 cattttcttg tggagaaaga agagggatca attgcaaatc ttctcgttct tcttctttgt
     3061 ccttcagttc aactggtata tcccgtgtga agagaatata ttcattattt tggcgtgctt
     3121 tagctaatct ttgagcaata cgttctgcca attgatctga ctctggaata taaaattcgc
     3181 tgtttggatc aagaatatga ttttgcatat caataataac taacattttc tttcctccta
     3241 cattcatatc gtcattttat tctttttaat agaagttttt taaaatataa gcaacaccag
     3301 cttcttcatt cgtcagtgtt acttggtttg caatttcttt tacttgtcgc tgtgcattcc
     3361 ccatcgcaat actcaacccg ctgtaattca acatagcaat gtcgttttct tgatcgccaa
     3421 aagccatgat gtcactcgat tcgattccaa ggtaatccgc cagaaaaaca agtgaatgag
     3481 ctttagtgat acctgtaggc gtgatttcta agaaatgcgg atcagagaat actgattgga
     3541 taccatctgc ttgcagatct ctttgtagct tctgtagttt ttcttcactc ttatctgtta
     3601 atgctaattt aagatagcta agagaaggat cttccaacat atttaaaatt tgatcaaaag
     3661 aatggttctg taaattgaaa tgttcataat aagggtcatc tttgactaga tttgattcat
     3721 cgaatgtcag ctgatcatct tgggtttctc ggctagcaat gatggatatt ttcgcttctt
     3781 gagcatagaa gaaggctttt ctcagtgtat cgctatctat ttttgaacgt ctgagaactt
     3841 tcgccatttt tccatccttc atttcagcaa tcagtgaacc attggaagaa atcaaatagc
     3901 caccgattcc taattcggaa aaaatatctt tagcagaaat gctgttcctt ccggtagcga
     3961 cggttacgat tccgccattt tcatcaaatt tccgaagtgc ttggatattt tcttctggta
     4021 tctttccatt tcgatcaagc aaggtaccgt ctaaatcaat cgctgctagt ttcattttat
     4081 cttcctactt tctttttaca tatctatttt cctgttacct taagattaaa tgagaattta
     4141 aaaaaatgaa actaacttgc tttgtcatta tgaattttt
//
LOCUS       DAYYME010000185         4080 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000185 DAYYME010000000
VERSION     DAYYME010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.187"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            48..581
                     /locus_tag="ACHBFW_002690"
     CDS             48..581
                     /locus_tag="ACHBFW_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318946.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(707..1384)
                     /gene="nth"
                     /locus_tag="ACHBFW_002691"
                     /pseudo
     CDS             complement(707..1384)
                     /gene="nth"
                     /locus_tag="ACHBFW_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
     gene            complement(1391..2089)
                     /locus_tag="ACHBFW_002692"
     CDS             complement(1391..2089)
                     /locus_tag="ACHBFW_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HFM9318947.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNDKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2357..3541
                     /locus_tag="ACHBFW_002693"
     CDS             2357..3541
                     /locus_tag="ACHBFW_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFM9318948.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3547..3990)
                     /locus_tag="ACHBFW_002694"
     CDS             complement(3547..3990)
                     /locus_tag="ACHBFW_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM9318949.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT         1079 a          790 c          658 g         1553 t
ORIGIN      
        1 cacttcttaa taatcccatt gttattatca aataaaaaat acaatatatg gttttgaata
       61 attttttgaa agaaggcttc attttgagtt atctgatagt atgcttaatt atcataattt
      121 gtctgcacca tcttttttta tcgaaaacaa tacctcgtta ttccaagaat aaaaaagctg
      181 aaaaattttg tttattatta aataaattta ctttaattgc tcctatcctt gcatttatta
      241 ttttttctgt acttttaagc actactctta aaggaaaatt tatggaaaga agctctcatg
      301 caatgattct cactttttta tggttgttgt tcaccagaat ctatattttt ctgatgagcc
      361 tgaaaccgcc caaatccatt agtttatgtt tagttataaa tggtattttt ttattatcac
      421 ttatcatttt tattacacct ttagatagat acgtcactta tttatataat cctttggaat
      481 actggactta ttttataggt atattggaag gcattatttt ctacatcggg tattttccca
      541 ataaaaataa caacttttat ttttatcgca ataaattgta ataagttacc atcctagcta
      601 ctgagtatgt acatttactt tttcatgcaa aaaagataaa cgaaacagct tttctccata
      661 ataacaagat aattcataca tttatttgca attaaagaaa ccacgtctac aggcgtggtt
      721 tcttttttag cgccttttct tttagacgca tccgattttt cccatcttga catattgata
      781 gcaaaggaca tacctcgcac ttgggatttc ttgccgtgca atgatatcaa ccaaaaaaaa
      841 tcagtgtatg atgtgtcttt acccataatt cttgtggtac tttccgcatc agtgtttctt
      901 ctacttccat gaccgtagca tctagtttgc agatcctcag gcgttttgag acacgttcca
      961 cgtgggtatc tactgcgatt gctggtatcc cgaatgcatc ccccaatacg acatttgctg
     1021 tctttctgcc tacaccagga agagacatca attcttctct tgacgttggc acttgcccat
     1081 cgaaacgttc aatcagctgc tgtgcacacg ctttgatgtt ttttgcttta tttcgataca
     1141 aaccaatcgt ttttatttta agaatgattt catcgattga agcttctgct aacgcatcgg
     1201 gtgtcggaaa agaggcaaac aaatctggcg ttgctttatt cactgataca tctgtcgcct
     1261 gggcacttaa gatcactgca atcaataact caaatggatt attgtgcttg agttctccat
     1321 gtgcttcggg aaacattcca tacatcgttt ctagagcttc cattgttttt tgtttgctta
     1381 acatattccg ctactcctcc aaccaatttt tcagtgagac tttcggcaat tcttcttgtt
     1441 gagctgactg ttctacttct ctttgaagaa gctgctgttt tcttcgtttt tggtcttctt
     1501 ccacttgttg ttttgtacgt agattttttc tttcccacga tagcaaaatg cggtcaacgt
     1561 aattaaaact ataagcctga tttaatacgg cttcccgcag tgctagcttc aaaatttccg
     1621 gctgataatg gtcttcttct agccattgtc cgatccgctg gaactcgatc gaagaaagcg
     1681 ggcgtccaaa ttcttgttca aataattgat acactgactg gatttctttt tcttgtgttt
     1741 tttcttcgta ttttttttct tgtgttttta aatactcacc taatagatca tagatggggt
     1801 aaagattata tcgatctgct tttttatcgt tgacaatcgt cgtttctatc ttgataaatc
     1861 catttgttac gagacgatcc agtatttgaa aaatctgatc ttgtttgatt cccatatcca
     1921 tagcaatcat ctgcaaatca gggaaagtat ctccttcaag ctgtgccata tgcaactgta
     1981 gaacaaataa aaattcttca gattgtagac cgatcctatg ataattttta agtaaaagat
     2041 tagatatgac tgtttgtcca gcgcttaagt aattttctaa attcaacatg atttctcctc
     2101 cgctcaatca cagtatacgt aaaaaaaaca tgggaagcaa gaacaggttt cttttctatt
     2161 tacttcactg atcggtcaga tttacaatgt cgtctacttg atttttgttt gttcatgttt
     2221 ttgatgcgta tcttcacttt tcggaataaa taaattaagt agatttcgct gttccttaga
     2281 atgttgacaa ggaaacaaaa ttactataca ctatttttgt ttccaagtta acattattgc
     2341 ataaaggagt ttttacatgt acagtttcac aaataaacat tctaaagttt ttggatacgt
     2401 ctctgtctta ttcactggat taggatttac tatcgtcagt cctgtacttc cgtttttaac
     2461 gctgccttat tctcactcca tacatcagca agctttctat atcactttgc tcatgtctgt
     2521 ttatgctcta gctgccttct tatctgcccc cattctcggt agcttaagtg atcattttgg
     2581 gcgtcatccg atccttatta tcagtttatt aggatcaagt atcggatatt tgctttttgg
     2641 tttaggaaat tcgatatgga tgttgttcct tggtcgtatc attgaaggat tgacggcagg
     2701 agaaatctcc accttatatg cttactttgc cgacatcacg gaacctaatg aaagaaccaa
     2761 agtttttgga tggatgggtg ctttagtcgg tataggaaca acgctgggtc ctattatagg
     2821 gggtctatta gctgagttgg ggaattctgt tcctatattt attggagcac tctttacttt
     2881 cttaaatgcc gtttatggct acacatttat gctagaaagc cttccaataa aaaaacgttc
     2941 agtagattta agtttctccc atgtaagacc ttttcaccaa ttacaacaat tattcaagat
     3001 ttcatccgtt attcctttat tgacagcagg ttttgctgtt tggttggcag ctggctctct
     3061 tcaatctatc ttttctcaat tttcgattga cgcctttcag tggaaggctg ggttggttgg
     3121 tttaagcttc tctttgattg gaatcttgga tactgtctcg caattgttca ttatgcctcg
     3181 attgctcaaa aaattttcag aacagcagat cacaagaata gggatgttca gtgaaatcct
     3241 cgcctatcta ttcatcacat tatctggaat tcttctactg ccgattttgt ttttatttgg
     3301 gattatctgt tatggtttcg gtgattctat ttttactcct gtattcaatg gccaactatc
     3361 caattctgtc tcagataacc agcaaggtct tgtaatgggc ggtacccaaa gtatacaatc
     3421 tctatcgcgc gtcatcggcc cgctaatcgc aggacagctt tatgctgcag caccatttct
     3481 tccgacggta ttcggatttt tgcttatgat ttttgcctat tatcactaca ataaaaaata
     3541 gtagcttcat ttgctacttc gtataaattc tctgtatcga ccgataaaac gaaatactgt
     3601 gtcaaattct tcttttgaca tattttcaaa tacagtagca tctcgctgta cccatttttg
     3661 atggagttgc tcgtgtctat tccttatttt ttgtccgatt ggcgtcaatt gaaaatagat
     3721 ttctttttta ttctcttcgc tttgataagt gtttatgatc cctctagcta tcagtttttt
     3781 tgttagctta ctgatggctc ctctagtcat gtaactagca ttcgctaatt ttgtcacgtt
     3841 aggatcaggg attttcccga tatgttcaat cacttctagt tcatttagag taaatcctgc
     3901 aaattctttt tccatttctg gtttattacg atgaataatc tcattagcta aatccagata
     3961 ttcttctatt agcagttgtc ttttatccat ttctacacct tctttttctt tcattatatt
     4021 gtttttttgt ttcctattca acaaagataa aaatattttg taggaaaacc aatctgaaac
//
LOCUS       DAYYME010000186         4067 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000186 DAYYME010000000
VERSION     DAYYME010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.188"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1157)
                     /locus_tag="ACHBFW_002695"
     CDS             complement(<1..1157)
                     /locus_tag="ACHBFW_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9318950.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
     gene            complement(1160..3409)
                     /locus_tag="ACHBFW_002696"
     CDS             complement(1160..3409)
                     /locus_tag="ACHBFW_002696"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM9318951.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            complement(3506..4009)
                     /locus_tag="ACHBFW_002697"
     CDS             complement(3506..4009)
                     /locus_tag="ACHBFW_002697"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9318952.1"
                     /translation="MENSIYLVTEPNTQLKDQDNLVSELSRQKQVYKFLELLLNSASY
                     LNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIK
                     EYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLG
                     LGIINGE"
BASE COUNT         1311 a          744 c          580 g         1432 t
ORIGIN      
        1 actctagtat tttttttatt tgtcgcaaaa aatgttacaa ataggatcac aataaatatt
       61 cctgcgaata caaaaaatgg agcagtcaaa tttgtacttc ttgttagaaa ttgtgctaat
      121 tgcatagcaa taggagctat aaaattacca atattacagc caataaaaat caatgaagta
      181 gcaaaattaa ttgtattctt tgtagtgact ttatctaagt tactaaagat aaatgggaag
      241 caccatgatc ctgttatact tgataaaaat aaacccgtta gcaggaacgt catattaccg
      301 tttgacacag caattaacaa atttgaaatt ataaagaata taattcccaa gtatagggtt
      361 ccttttccta tccacttatt tattggtcca aacataaatc ctgctagtat tcctaagata
      421 ggcattagag ctaaatagtt actagcatta aagtttaccc ctttaatttc agttgcaatt
      481 gatgcaaatc gtacagaaat agcaatacta ttcattacga gtaagattgc aaatagaact
      541 agggcaaaaa ccattggatt catcttttct tttacatgat tttcaggtat gtcactattt
      601 tcatctctaa tttcaggaac ttttagcgcg aataagagag ctacaggaaa cgcaattgca
      661 tagactaaaa acgaagccgt ccaattaatg ttcactaaaa ctccagcaag aaaaataagg
      721 attgtctgac ctaaagcttc cattgaattt ctaataccta atagcgttgc tctagtattg
      781 ccagagtata aagagctgat atatgtcaca gccaatgaat tatatactcc taaaccggca
      841 cctaatatta ctcgactaat aagaacggtt tggaaggtag ctgtaaatac tggaataatt
      901 cctcctatac cagctaataa taaacctaaa attattgtct ttttcattcc tattcttttg
      961 gcaaagtaac tagataataa gataaacaaa ataacagtta tacttggtgc agttcctaaa
     1021 agttcacttt gactttgtga tatccccaaa gactgtttca tttgtggaat aacaccatta
     1081 attgcttgag agcttgttaa cattagtgaa atacttaaga ttgctccttt ttgaagtaag
     1141 cttttttggt tattcatctc tacttttcct cctgtgttat aacccaaatc ttggaatcaa
     1201 aatcaaatgt ttctgggcgt cgattctgat catttatttg tccactagat gcatctgaac
     1261 agactagtcc tgctctcatc aattccattc cagatcgcaa actattttgt cctacattat
     1321 aaagtccgtc attatctaac ccttttaatt tcagtcggcg gtaaggagca tttacctcat
     1381 ttaatgtttt ataatgagca acaatagccg ttttcttatc atgactaaca accatccacg
     1441 cggtttgatt atcatcatcg aatggtgata atagtctata aaaagtacca ttatgaatta
     1501 tggttctata ttttttataa aaggcaattt gttcttttac aatcctcaaa tcgccttcac
     1561 ttaattcatt taagtcaagc tcatatccaa atgcaccaaa aaaagctaca tttcctctag
     1621 tttttatagg tgttatacgg tttgtttgat gatttggaac aattgataca tgagcaccaa
     1681 tagacgaaag aggatacagc atactagttc catattgaat ttttaacctt tcaattgcat
     1741 ctgaatcatc actagcccaa gcttgaggtg catagtgaag cattccagga tcaaagcgcc
     1801 caccacctga tgaacaagac tcaaatagta tgttaggata ttttgttata agtttttcat
     1861 ataagcgata tacgcctagt acatatctgt ggaatacttc tccttgttga tctgctgaca
     1921 agtgactgtc aaatacatca gtcaatggac gattcatatc ccacttaatg taatctaagt
     1981 ttgattcatc aatgatttta cacatttgat cgaaaatatt ttcaacaacc tcatctaacc
     2041 caaaatttaa tacatattgg ttcctaccat gattaggttt tcgattaggt gtagaaataa
     2101 tccaattcgg atgtgtgcga tataactcac tatccttatt caccatttca ggttcaaacc
     2161 ataaaccaaa tttcatacca aaactacgaa ttttttctga aaggctgccg attcctctag
     2221 gtagtctttt aggatttaca tgccaatctc ctaatcctgc acaatcgttt tctctttctc
     2281 caaaccagcc atcatctaat acaaataatt caataccaac atcaaatgct tttttagcaa
     2341 tatttaccaa cttttcttcg tcaaaatcaa aatatgttgc ttcccaatta ttaattaaga
     2401 caggacggtc tttttctcgc cacttaccac gagctaagcg ttttctaaat agttgatgaa
     2461 aagtttgact catggcattt aaacccttac ttgaataaac cagaatcgct tctggtgctg
     2521 taaatatttc tcctggattg agtttccaat taaatccaaa tggattgatg ccaacttgta
     2581 gtctagtaac atcccatgta tctacttctg cttgaattaa gaagtttcct gagtaaacta
     2641 gtgctgcacc aatagcttct ccttgatttt catcagtatt ttttcttttt aaagtaacaa
     2701 atgggttatg ctgatggctt gaaatacctc gagtagattc gattgattga attccttgtt
     2761 gtaaagtacg cgacttaata gttctttctc taccccaagc accagataat tgttgccatt
     2821 cataatttgc atcgggtaaa tccaatgata aactgctaat attctcgatt atttgaatat
     2881 cttttccttt attcataact cgcgcgctac gagtaataac aggataatct ctataaattg
     2941 tatataataa ttctacttga acatttgtaa ctgaatctaa aatataaact ttcaatgttt
     3001 tgctttcatc attattatca acataggtag caggtaaacc ttctagttta ggttttcctt
     3061 cttccacttc gtacccttga tagacaaaat ctgataagcg agagccattt ttctgtagta
     3121 atgaaatagc aggatgtcta aaatctgttg taccatattc aggaaattct tgttttaagt
     3181 gctctaacga atatagcaaa tcgttttcat ttctataggt tgtcataggc cgatgatgca
     3241 tctcaattag atgactatag tcattatctg gtaagttctc accataatat aaatgcaata
     3301 atttaccatc ttccgatatc ttaaaaatgt aacttatctc ctgattaaac aaatgaaatt
     3361 gttggttttc tgatcgataa ataatattca tttcattatc cttatacata agctctcctc
     3421 cgtttcttta ttgcattata aaaaacgctc acataaatag taagcgttta aagtaacgaa
     3481 tatttatcaa aatcaaatgt ttagtttatt caccattaat gattcctaga cctaatctac
     3541 gttttcgata tactcccggt gaaacaccat ttgctttctt aaaggcttta gaaaaagatt
     3601 gtactgttga gaatcctatc ttgtcactga tttgctggat tgaatattca gttgatgata
     3661 acagatcgtt tgcaatagaa attttgattt cactaatata ctcttttatt gatattccca
     3721 tatcattctt aaacaatcgt gataaataat tttcgtgtat cgatagactg acagcaatat
     3781 ctttaacaga taatttttga tacgcattct cacgtattat ttcaattgtt tgacgaacaa
     3841 aaggattcat ctttttagta attatccctt cgttcagata actagctgaa tttaagagga
     3901 gttctaaaaa tttataaact tgtttttgtc tggacaattc actgactaga ttgtcctgat
     3961 ctttcagctg agtgttaggt tctgttacta aatatataga attttccata tccaccctcc
     4021 tatgttagat ttggataatt tttgttttat tatgtttata ttattac
//
LOCUS       DAYYME010000187         3997 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000187 DAYYME010000000
VERSION     DAYYME010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.189"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1434
                     /locus_tag="ACHBFW_002698"
     CDS             85..1434
                     /locus_tag="ACHBFW_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9318953.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="ACHBFW_002699"
     CDS             complement(1562..2152)
                     /locus_tag="ACHBFW_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM9318954.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHBFW_002700"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHBFW_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM9318955.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1141 a          737 c          784 g         1335 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
     3721 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
     3781 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
     3841 acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     3901 gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct ccatatactt
     3961 tgccgttttg ttttgaacta ataccgaaga gaagctg
//
LOCUS       DAYYME010000188         3979 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000188 DAYYME010000000
VERSION     DAYYME010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.190"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACHBFW_002701"
     CDS             complement(<1..300)
                     /locus_tag="ACHBFW_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFM9318956.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACHBFW_002702"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACHBFW_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACHBFW_002703"
     CDS             complement(1014..1304)
                     /locus_tag="ACHBFW_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318957.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="ACHBFW_002704"
     CDS             1706..1942
                     /locus_tag="ACHBFW_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318958.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="ACHBFW_002705"
     CDS             1949..3901
                     /locus_tag="ACHBFW_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM9318959.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
BASE COUNT         1383 a          679 c          694 g         1223 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactc
//
LOCUS       DAYYME010000189         3978 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000189 DAYYME010000000
VERSION     DAYYME010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.191"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..1986
                     /locus_tag="ACHBFW_002706"
     CDS             46..1986
                     /locus_tag="ACHBFW_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM9318960.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            2073..>3978
                     /locus_tag="ACHBFW_002707"
     CDS             2073..>3978
                     /locus_tag="ACHBFW_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9318961.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTL"
BASE COUNT         1339 a          686 c          838 g         1115 t
ORIGIN      
        1 agaaaccgtt tttatatact ataaatgtag aaaggcggta ggaaaatgaa aaaaattatt
       61 gcaatcacat cctgtccagt tggtattgcc catacataca tggcagcaga aaacttagag
      121 aaagcgggga aagcagttgg cgccgaggta aaagtcgaaa cacatggttc catcggtatt
      181 gaaaatgaat tgactgcaag agatatcgaa gaagcagctg gtgtaatcat cgcagcagat
      241 actaaaatag ataaatcacg attcggcggt aaacctttga taaccgtagg tgtgcaagaa
      301 ggaatccacc atgcagatga gttggtgaga gatattttag caggaaaagc gccagtctat
      361 aaaagtacgg aggcaattat ttcttccgaa aataaaagtg aatcagaaaa tttaggtaaa
      421 aaaatctata agagtttgat gaatggcgtt tcgtacatgg tacctttcgt agttacagga
      481 gggttattga tcgcgatttc tttaacatta ggaggaacag ctacacctga agggatcaag
      541 ataccagaag gaacgatttg ggcaacgatg aatagtatcg gaagtatcgc tatggggttg
      601 atggttccaa ttctatctgc atttattgcg caaagtattg cagatcgtcc aggacttgtt
      661 cctggatttg ttggtgggat gttagcggca aacggagcat tatatggtag tgatgcgaat
      721 gcgggattct taggcgggat catcactgga tttttagcag gattcattgc tttaggaatc
      781 aagaaagtgc gagtaccgaa agctttacaa tcgatcatgc caattattgt tatcccaatt
      841 ttaggatcat tggcagttgg ttttgtgttt atctatgtta ttggagaacc agtagctctt
      901 ctatttagtt cattgactca ctttttagca gggatgcaag gaggaagtga aattgttcta
      961 gctatgatct taggagcgat gattgctttt gatatggggg ggccgttcaa taaagtagcc
     1021 tttttgttcg gatctggctt gattgcagaa ggcaattatt ccattatggg gccaattgct
     1081 gtagctatct gtatccctcc attagcattg gggctggcat cattgatttt aaaaaccaaa
     1141 tttacgaaaa cagaacgaga agctggtaag gcgtccttag caatgggact gtttgggatt
     1201 acagaaggcg ctattccgtt tgcttcaaca gatccactac gggtgattcc aagcattgta
     1261 atcggggcaa tgacaggaag tgtgatcgcg atgctttcag gagtgacaga tcatgtagct
     1321 catggcggtc cgatcgtagc ggttttagga gcagtggatc acgtagccat gttttttgta
     1381 gcagtgattg ctggtattgc tgtaaccgtg ctaatgttaa gaatccttaa accgacatta
     1441 caacaaactg cggttgctgc agcaaatggt gtgacagtag cagatctatc agagactgaa
     1501 acaagtgatg tttatacgaa aactgaaact tttgattcta tcacggatat tatgtcagat
     1561 gaagcaatcg ttttaggtga aacagcaaca acaaaagacg gagtaatcga agatttagtc
     1621 caacgtttag aaaaaacagc ggcgatcaca gataaaacca gatttaaaga agtcatctat
     1681 gatagagaac atgaaagtac aacgggaatc ggtatgggga ttgctattcc acatggaaaa
     1741 tcagcttatg ttcttaaacc aacagtggca tttgccagaa gtcaatctgg ggtagattgg
     1801 cattctttag atggaaaacc tgcacatatg atctttatga ttgctgtacc tgaaaacagc
     1861 caaggtgata tgcacttgaa aatactgcaa cgtctatcac gtaaattgat ggatgatgac
     1921 ttccgtcaag cactgatgga agcgccagat aaaacaacag ttcaccaatt attaagcgaa
     1981 atgtaacata agatgaaaaa tcgaccgtga tgagacataa tagatgtgtt atcatgatcg
     2041 attttttatt tagaagggag tttctgcaca ttatgaatca gcgtcagcaa tctttgatct
     2101 atcaattaag tgagaacaat aattatataa caacaaaaaa gctagcagag catttccatg
     2161 tgtcagaaag aacgattcat agcgatttga ataccattga aacttggttg agagacaaac
     2221 atctgccttt aatgtttgaa agaaaaaaag gtacaggtat cttgttgaat ggtctgcctc
     2281 acgaaaggga acaactgaaa cgagcgctta atgaagagac tcaaacagaa atggataaaa
     2341 aacagcttcg tcagttattg attttccatc tgctagtagc aaaagacgga tttagtcttg
     2401 aagaactttc agaaaaacta tatgttggaa aacgtacgat tcgtaaagaa ttgacagagt
     2461 tgcaaagctt ttttgagtat caccatttac agctagtatc caaaacaaaa ctaggaactt
     2521 ttcttaaagg cgatgaacaa gaaaaaagac agttgcttgt taagacatta cgaaatatgc
     2581 agaaagaaga tccacaaagt ccgtcgttga aagaattctt cgctaaagat acattaaaag
     2641 ttatccaaca tacattacaa gatgtttttt atgaaaacca tttagaacag ccgaatggac
     2701 tggctagtgt ggagattcac atctacttta tgctcgagcg aatgaaacag taccagaaag
     2761 tgaaattaag taaagacgaa aacgaagtcg tggaacacac ccaagcacaa aagttaagta
     2821 gtcaaatatt agcaaaacta gccaccatct atccaattga attttctcct gatgaaatca
     2881 attatctggc tttgcgtatc gccaatcttt ttacaaattc acaagcagcc acacgttttc
     2941 aaaaggaatc tagcgcactt gctgatcatt tgatcgaaca agtggaacag tttctaggct
     3001 attcattaaa agaagatcag ctactaaaac aaaatttaag gtctcattta tcttcaactt
     3061 attttcgact ccattatggt ttgcagattt ctaatccatt gacgaaaaat gtgtttagta
     3121 cgtacacaca acttttctta gttttacagt taattttaga agattatttt gaaaaggaaa
     3181 atttttatgt tccacaagag gagacagctt atttaacgat ccatttccaa gcagcgatcg
     3241 agcgacacaa acatcagaaa agtagaagat atcaaacggt tttagttagt gaatattcaa
     3301 aagctatggc tacttttctc gaagcacggc tgaacagaga acttccagaa ttaacgatta
     3361 tggatctagt tgaatataag ccagaattaa agagacaaga ttttccctct gttgatttta
     3421 tcttggctac gttaccattt aaacatataa gcattccagt aatcgagatc tcaccaatga
     3481 tcacagaaac tgatctagcc tatttgacaa aatatatgct ggagcatgtg cctatcaaga
     3541 agaaaaaaac atttgatcta gcaagcttta ctcatccatt tctgatcttt cctcaacttg
     3601 aacggacaga cccagccgat attttaaact ttatgggaaa cgtcttagtg gaacatcatt
     3661 atgtggagcc ggaattcatc gattctgtct tggaaagaga cagtcactcc agtactagag
     3721 tagcaccatt tgtaacaatc cctcacggta atccgctgta tgtgaagcat tcgatgattt
     3781 ctattgctac aatgaaagaa ccagtcctat ggcacgggga acagatacgt atcgtactgc
     3841 tattagcaat aaagaaaaat gatttgaaac atgcagagtt caaaaaaata ttttctgtta
     3901 ttcactatct agaaaagcag ccagaacaaa tggctcagtt gatgcaatcc aatcatccgc
     3961 tagaaatctt aacgttgt
//
LOCUS       DAYYME010000190         3901 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000190 DAYYME010000000
VERSION     DAYYME010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.192"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..606)
                     /locus_tag="ACHBFW_002708"
     CDS             complement(64..606)
                     /locus_tag="ACHBFW_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFM9318962.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            741..1130
                     /locus_tag="ACHBFW_002709"
     CDS             741..1130
                     /locus_tag="ACHBFW_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318963.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1134..1898
                     /locus_tag="ACHBFW_002710"
     CDS             1134..1898
                     /locus_tag="ACHBFW_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9318964.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIQLMEEASTFLAKNLIKKYK"
     gene            2027..2974
                     /locus_tag="ACHBFW_002711"
     CDS             2027..2974
                     /locus_tag="ACHBFW_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9318965.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3104..3844
                     /locus_tag="ACHBFW_002712"
     CDS             3104..3844
                     /locus_tag="ACHBFW_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318966.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1294 a          666 c          799 g         1142 t
ORIGIN      
        1 aaataaaaac tgtctaacat ttaaaattta atttagatat tagacagttt ttaagtttgt
       61 gagttatttt ccttgtcgtt ttctcacttt tttagtccag aaaccacctg aaatatattt
      121 tggctcataa gaaatgataa aagcacgctc ttcgatttct ttgatcaggc gatacagttc
      181 acgttcgctt tttctaggag aaagtatttc gagaactagc cgctcacctt ccagccccat
      241 tgcagaagtc tgtgtcacac cataaccgtg ttctctcagt atacccggca aacttttatc
      301 ttctgcgcta tttgctggca aaatcgttgt gaccatgatg tatcctaatg ccaatttgtc
      361 ttcaatcaaa attccgacac tgatacctac tgcgtaacct aacgcataga caaataaatt
      421 cagtggatta tctaaacgat ctaaaacaag ggataagccg acgatataaa tcgtgatctc
      481 gaccatactg acaagtggag caatcagtcg atagcctttc attgtcaaca tgaaacgtaa
      541 cgtgttcaga gtgatataag caaaattaat gaggaagatc atccccaaca tttttggatc
      601 aatcatttgt aaaactcctt tcatttatat aacaacaata tcaaaaaaaa tcgtaagtga
      661 agaaaaattg aaactttata catacaaaaa aatgataaaa tgattataat agaattataa
      721 agataggtta ggtgattaaa atgaaaacgg tcaatcgtgt gttttgtgcc agccacgtgc
      781 taagtctcct tctcagtata tacttaggtg tctattcgat tcaacagcca attgaggtag
      841 agaacattgt ttttcagatg tcgctgacag atggtttaat tttaagcgtt tttttctttt
      901 tgatactgtt tattggtaat attttcggta gcgttgccag ttttttgaat ttttctattt
      961 atccactcct ttcgctagca ttaggagtgg tgggactttg ttctctgtgc ttatttcccg
     1021 agccattcag tcctgcattt atattatttg gtacactgaa cctgttccag acaactgttg
     1081 gagcatggtt gctatggcga tcaggaaatt taatgaaaat aggtgagtaa caattgaatc
     1141 caattatatt gaaacgttct gtccatcata ttccagtgtt agaagttgtt ctgaaagaat
     1201 taagagagga acgtattccc gttgtcatct attatcacgg atggcagaca agtaaggagt
     1261 tagttttgac gcaaggaaga aaaattgcgc agcagggcat tcgtgtgatc ctgccagatg
     1321 cgatgaatca tggggagcgc aaacagccgg tttcttctat tccatcctat acattttggg
     1381 atagtatcta cggaaatctg tttgaatttg acacacttat caagcattta gaaaaaagag
     1441 gtcttctaca tgatcagctt gcagttggcg gggtttcgat ggggggaatg acaacatgcg
     1501 ccttgttggc taaacatcct catatagtag cgggcgtgtg tttgatgggt tcccctgcac
     1561 ctttggaata tggaaaagaa atcgtaaggc gcgcatcgga atataattat gtcgtaccaa
     1621 aagactattt tgatttgatt tcatgggtta agagctatga tttgtcattg cagccggaaa
     1681 agttagctgg acgtcctctg ttcttttggc atggacaagc tgacgaaaaa atcccttata
     1741 tacaaacgga acgatttgta gaaaaaaatc ggcataagtc ttacggaaaa gaaattgttt
     1801 ttcgttcctc aaaaacacag cgacatatgg tacaaataca actaatggaa gaagcttcca
     1861 cttttttagc aaaaaatttg ataaaaaagt ataaataatt gaaacggaaa atcactcatg
     1921 acgatagagg attttccgtt ttttattttg ggctataata aactctgtta ggaaaatttg
     1981 gtataattga tagtaagaat gacaacatgt tagaaaggaa gagagcatgc ccactttagc
     2041 agatgttgca aaacgtgcca atgtttcaaa aatgacggtc tctcgagtca tcaatcatcc
     2101 ggaacaggtg actgacgaat tgaaagagtt agttttctca gcaatggctg aactggatta
     2161 ccgtccaaat attgctgcaa aagcacttgt cacgaatcgg tcgcagatca tcaaattatt
     2221 tatactggaa gaaatcgaca caactgagcc ctattacatg aatttgctga tgggaatcgc
     2281 taaacggatc ggtaaagcgc attattctct ccaattagtt acaaatgatg ccttcgacgt
     2341 cggttcttgt gatggataca tcatcacagg tgtcagaaaa aaagacttcg attggatcga
     2401 ccgtttggaa aaaccagttg tcttgtttgg ggaaaattct gaaggatatg attatgtaga
     2461 cagcgataat gaatatggta cagcaaaagc aacgcaatat gccttagaaa gagattatca
     2521 aacgattatt tatatcggta tcgaagtgga cgagcctttt gaaatttcaa gggaaactgg
     2581 ttatttatct gtgatgaagg aagttgatct tcctagtaaa atcttgcggt ttccaaatca
     2641 ttcgacagaa gtagaaagat atatgacaga aaactgggca gattttacag ataaaacttg
     2701 ttttgtatgt agttcagatc ggctagcact aggagcagta agagggatta taaatgctgg
     2761 aggacacttg cccgaagaat ttggtgtgat tggatttgat ggggtatttt tagaccaaat
     2821 cagttcacct aaattgacaa cgatcaagca agatattata cgtatgggtg aagtatgtgg
     2881 agaaatgttg ttaaaaaaaa tagaagaaaa aggggcgagt caaggttatc gtcgtttttt
     2941 tcctgaacta gttatcagag agacaacgaa ttaagtctta ttactatata tatatttatg
     3001 aaattcatcg ctattttctt aaaataatca gcgcattgct actgaaaaca atgaatctgt
     3061 gctattattg agaaaagttc catttttgag aaggatggtt tgaatgaaag aaatcagacg
     3121 aaaacatttc cgttttcctg cgtacgatga tgagttaggc gtaaaattaa ccaacgacaa
     3181 gaagcgagta ttatttgacg gacaagaaga tcttttgact gaacagaatc agtccccgtt
     3241 tgaacagatg aaagaatttc atttcacgaa cacgaacacc gagaaaatcc gtcgcaagcg
     3301 tcgtgcaaca aatggattga agtcagctcc gcgtaaggaa aatttggaaa agcataaagg
     3361 aaaattaccg gattataccg gccattacca atcagaaatc acatcgacag gacgaaaaaa
     3421 actttttggt gattcctctc gttctgtcag acaaagtgaa acaaggacag agtccaagca
     3481 tactacacga aacgcatctg ttgacacgaa ggtgcgggag cgttcttatt ttgttcctaa
     3541 atatgtaccc gcatcgatta tcccagaccc tagagaaagc aaaatttctg aggatcattt
     3601 aatcgaatcg atgaaaaaaa cggatgactc ttatctgttg ttcgatacag aaccgacgcc
     3661 gtatcaagaa aaaagaaatg gtgagccgtc tgtacgaaca ttcaatcgaa caaaaacgtt
     3721 agaggaaaca aagcagtcag aagaaaaaaa atcgaaaaaa agccatggta tcttagaacg
     3781 ctcattacaa ggattgatcg atgatcaagg aaactctttg ggagaaaata gctattttaa
     3841 ataagtgata tacaaattta tattcggcta cagttggaaa ggaaacgctg cggcgtttcc
     3901 t
//
LOCUS       DAYYME010000191         3877 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000191 DAYYME010000000
VERSION     DAYYME010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.193"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(717..3866)
                     /locus_tag="ACHBFW_002713"
     CDS             complement(717..3866)
                     /locus_tag="ACHBFW_002713"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFM9318967.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          636 g         1362 t
ORIGIN      
        1 gactaataca ggtagtcctc ttttttgtta ttttgatatt ctaaatcatt ttttattgga
       61 ttattccctt gttcgatata gacccaaatt gatctatcaa taattatcaa actgagtcat
      121 atagagacaa aaatttcaaa aatgacctgt taaatggtca tcgaatatca tttaaaatca
      181 atacgacgga gtcagacacg gagtcaaaac aagcccgctg agagtaaaaa atatatacag
      241 tcccataggt aaagtgccac aggactgtat atttttttat ttgagattac ctgaaatgat
      301 tccatttact ttttatctca ttttttccta ctagttagta tcaaatactc tcctattttc
      361 tcaataaact attttgggtt acatggtatc tgaaacagct aaaaccttgt cattacagca
      421 aaaaatcact ttatctccgt ttcatttcag atacatttca cttaagcgga ttccaaaaaa
      481 acaaaagaaa aaggaacccc tagttagcct atgcttttta ttggttcctc tcaaaacacg
      541 gtcatataac aacggggtgg ggtaaatgaa tgaaaaaggt ggaggaaaca ttattcaata
      601 gctgtcttaa atacttcgtt ataaaatgtg aacaaataaa agacatctat ccctattttc
      661 tacacttcca ctttactacc atctttgaat cgtacaattt tcttaactct tctcgtttac
      721 ctcaattcat tttcaagtgg aatcagctca tcatctaaca ttttacgcca tgctcgcttc
      781 atttcttgtg ggtatttaaa tggcttcgct tctggatggt tggctttata tccttcgtaa
      841 tccttactgt tgataattgc tttcatatta ggaattggat cttcacctgg catatattgc
      901 agattagaag catgtaattc tttctcagaa acaaaccact cattggaaaa gacatgaatc
      961 aaatcatcac tcgcttgtgt aaaatagcga cgtttaatga caaagatatc ttcattacta
     1021 tctaaagtcc ccacatcaaa cgcatttaaa atttgtcggt aaacttcttt gactccttct
     1081 gttttattca atttagccaa agctttttca atttcttctc gtgcatctga actattcgcc
     1141 gcataagttc ctagtaattg atcaatataa gtagaatcaa tatcatacaa cttcgtggta
     1201 ttttcactat aaaattcgat cgtggaaaaa tcttctccct ctttttctgg ttcttcctct
     1261 attaatgaac ccttcacggt ctcgtacacg ccaatttgtt cttccaaaag ttgaatttga
     1321 ttgttcaagg ttgaagattt caccatgtca tcattgtaat cgtcataagt cactaacgat
     1381 tcataagcac gattcaattc ttggaatgct ttgacgtact cgatacggat ttcaagtggc
     1441 gtatgttcat caatggattc catggatgaa acctctttta acttaccata tgcttgcttg
     1501 aatctttttt tggattcctg ataacttgga taaatcaaac ccgattcttc ttttgcttct
     1561 gaatacagtt ttgtcgcatc ctcgacattc ttctccattg tggctggctt tcggaaagta
     1621 acaattagtc cttttgtttt ttctggatac gtacggttcg tacgtgaaaa agcttggatc
     1681 aatcctgcat actctagatt acgatccaca aatagggttt gaatcgtagg tgcgtcaaaa
     1741 cctgtcaata gacgatccac tacgatcact aaatcaattt gtttcccaaa ttgtttgaat
     1801 tccgctcttt ttcgtgccaa acgattattg atgtctccgt tataacgctc aatgtctgct
     1861 aatgaccagg aagtatcata atactgatta tattcctcaa taattttttg catttcttct
     1921 tgttggacac ttgaatctcg gctgttttct tctaatgaat aggtaatagc gattcgtgga
     1981 aaatcaggat cctccatcgt tcttccttca cgaatgggct gattaggaaa ctcttttgtt
     2041 aaccagtcag gttctttcgt catttctttg atcgctcgat aatacttttt ggccatgtca
     2101 atggaactcg tggttaagat cgcagacttc gttggacggc catttcgaaa atcaaatttt
     2161 gtataggcat tgtctggacg gaacatttta tgaatgactt tttggatatg ttcatctgct
     2221 tcaaaaacag tagggtccaa ataactttct tttttgacag gctccatttg atcaatcata
     2281 cgattgatct gttccggtga atactctgca tatgtttcta cttcttttaa tttccgatag
     2341 attttattct ctaatgaagt cggctccacg gtgttttcat gctctacttg aaatcctaaa
     2401 accgagccat cttctaatgc attcttgatg gtataggtat gcaagacctc tccatactga
     2461 tcatgggtcg tacgtgccaa ttgccctttg gcttgtttcc gattctcagg gaaaatcggt
     2521 gtacctgtaa agccaaacca agtggatttc ggaaacattt ttttgatttc ttccatattt
     2581 tcagcactta acgctcgatg acactcatct actacaaaca cgatatgttg ccccattaat
     2641 ttttggaagc gattggttcc ttttttctct tcttgttttt ttgcatattt caacgccgca
     2701 tctagctttt gacgggttgt aataatcaca acatttgcat tggcatctgc caacagagtt
     2761 tcacttaact ctttggcact tcctgttccc acgatcaagc tattcgcttt ggcgttcccg
     2821 gaagaaatcc cagtattaaa ttccgaagca aatttcgtaa actctgtggt ggtttgatta
     2881 tctaaatctt tgcggtccac taacatgatc gtccgatcga tccccgattt tcgtgctaaa
     2941 agcttagtag atacaaaact agtcaacgtt tttcctgaac cagtcgcatg ccacacatat
     3001 cctgattgat gtttatttgc agcaataaac aaggcttgaa tcgcatgtac ttgataagga
     3061 tgtaacacca tgaggatttt attgtcttga tcttcactga caatcgtata atccgcaatc
     3121 aagcgatgag catccgggat atttagcact tgtttgcaaa actcatataa gttttctacc
     3181 tttttattat ctttcgttcg ccaactaaat aaaaacttag gatgcaaatc ctttggtaag
     3241 gcatttgcaa aataccgagt ggtttgttca ttggaaacca caaacaactg taaggtcgaa
     3301 aagatattgt tccgaaacat accttcttct gcatattttt tgatttggtt aaacgcctga
     3361 taaaaactat cttttgcggt tacttgtttc agctcgattt ggacaatcgg taatccattg
     3421 atcagtaagg tgacgtcaaa acgtcgatcc cgtgcttcta tgttacttcc acgttttgca
     3481 atttgatgga ccacttcata ggtagagatt cctccaccaa tgtcttgatt agaataaaga
     3541 atgagcgaga cagaacccag ttgcggatct tcccgttcaa tcgtgatccg agccattcca
     3601 ttttcacctt ttaaccattt cgcagcatca aagggggttt tcgttcgtag taacaattct
     3661 gttttgatcg tttcaaattc tttatcggtc aaaggaaaat cattcagttc tgcttgatta
     3721 tttgagatga ttttttggcg taaattgacc caaagatctt cttctgattt tagatcggga
     3781 cgatacgtcc actgattatg cccttccccc aatacacgga tcaactgttc ttcgacttcc
     3841 gcttcctcac ggtgactaat tttcatttat cttccct
//
LOCUS       DAYYME010000192         3718 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000192 DAYYME010000000
VERSION     DAYYME010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.195"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..353)
                     /locus_tag="ACHBFW_002714"
     CDS             complement(<1..353)
                     /locus_tag="ACHBFW_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318968.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRT"
     gene            complement(613..1161)
                     /locus_tag="ACHBFW_002715"
     CDS             complement(613..1161)
                     /locus_tag="ACHBFW_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318969.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1162..2019)
                     /locus_tag="ACHBFW_002716"
     CDS             complement(1162..2019)
                     /locus_tag="ACHBFW_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM9318970.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2305..2591)
                     /locus_tag="ACHBFW_002717"
                     /pseudo
     CDS             complement(2305..2591)
                     /locus_tag="ACHBFW_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2581..2910)
                     /locus_tag="ACHBFW_002718"
     CDS             complement(2581..2910)
                     /locus_tag="ACHBFW_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM9318971.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2987..3259)
                     /locus_tag="ACHBFW_002719"
     CDS             complement(2987..3259)
                     /locus_tag="ACHBFW_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM9318972.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3259..3522)
                     /locus_tag="ACHBFW_002720"
     CDS             complement(3259..3522)
                     /locus_tag="ACHBFW_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM9318973.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1091 a          754 c          473 g         1400 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac tctatttgag agtcatctaa ttggctcata
      241 atcctttctt tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac
      301 atactcaccc caacaaccaa tcctaaatat attttctttt taaacttttt catctacatc
      361 tcctcctaat agttttattt tagagttagc tctattactt atttactaac tcaacaacag
      421 attatcatac acttgttaat gaaatcacaa tatctcatta aaacacaccc taaaatcata
      481 accgttccta tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct
      541 ctatttcacg caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt
      601 cttctttgat tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc
      661 ggtatcttgt tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg
      721 aacaattgga tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc
      781 ttgcttatct tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa
      841 ttcatctctt gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa
      901 caaagtcgac agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg
      961 tgcgccaact ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat
     1021 ttcttgaact tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt
     1081 tttctttgct tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc
     1141 aagtcctttg atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa
     1201 ttctttcaaa atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc
     1261 tttctttgtc ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt
     1321 gagtacaaca tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc
     1381 ctgatctact gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt
     1441 tgtatcaaat tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat
     1501 cgattttact tgggtctgta agacgacgat tgcccaatcc gcaatgtaca tcgcggattt
     1561 actatataca gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa
     1621 tggttctagc ttttctttca tatatttgaa ttgggataat gaatcattat attggtcttt
     1681 gatccaatca tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat
     1741 tactttatag acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt
     1801 ttcgtaaaca atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt
     1861 taagttcatc acatcagaag tatttccttg catatccaag tcaattatca aggttctttt
     1921 tccttcatta actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc
     1981 acctttaaaa ttaccgatta catatttctt cgctaccacg ttcctacctc ttttctttta
     2041 gcaatattca tttgatacta ttataacata tttattttta gttactacca aaaaaaagat
     2101 atatatgtac taccttataa ttaataagta aaaggtagta cataaataag gaaaagctga
     2161 aatatattga tttatagctt tcctcaaagt aatgacgtta tttaatttat cgagaagcag
     2221 agaatactcg cgactttagt catgtagacg aatcagattt tcgataaaaa agaagccgtc
     2281 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
     2341 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
     2401 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
     2461 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
     2521 tttcaatggc ttctctttta gctttttaaa tacttaatga catgttttga aggaacgatt
     2581 tcataagcca atttctgttg ccaactcctc tttagtcatt actttagaat tagtaagtgt
     2641 atcttcagca atatctctaa aggaagaatg cattttgttt tttctattct taaattctac
     2701 aagaatctca tctttatcgt agccttctgc aattatatca gacaaaatat cttcgctaaa
     2761 atcataaaat tctttttgtg gttcaacaaa ttcataaaga ataccattct ctaccaactt
     2821 tgcttcaact tcgactccat taggaacatt aaaatcttta ggtactgtca acatgattga
     2881 attgccttgt tttcttgttt ttgtaatcat ttaaatccct catttctaac agtatgtaat
     2941 tacttagtaa atacattata ccacgagata atgtatttac aagtaattac tgtgactcgt
     3001 aatgcgtcct tgctgaataa atttcaacca cgtgttcatc ttcatctacc ttataaacaa
     3061 cccggtgttg tctattcaat cttctagaat acctaccttc atgtgtaggt tttaacttct
     3121 caaatgattg ggtagggaga tagggatctt cctttaaagt ctgaataacc ttctcaaatt
     3181 gaggtttcaa attagactgt ttgattttct ttaaatcagc tttagccgag tttttaatca
     3241 tcacagaata gttattcatt ataagttatc ccaatcaatt tcatcaacat tagtaaatcc
     3301 actatcatct ttttctcgct tacgaacaac acccaaagtg cctatttgct caagtaatag
     3361 cgtttctttg attgctttcc aatcatccaa accaataagc accgcattat tttcactgcg
     3421 ctcactaatg atttcaattt ccgtatgatt ctcatttaca tctttcaaca acttataaaa
     3481 atttttccgt gctgagctag gatttaagac tgtttttgcc atatctactc cccctctaat
     3541 ttatatctcc attctaacgt acttaattaa gtacgtaaat tcgattgttt tgtttaaatt
     3601 ggagtaccaa aacagcattg taaagtgtta tccgtttcct ttatacccgt ggtgctagtt
     3661 aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa tcaacaaaaa aacgcata
//
LOCUS       DAYYME010000193         3717 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000193 DAYYME010000000
VERSION     DAYYME010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.196"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..155
                     /locus_tag="ACHBFW_002721"
     CDS             72..155
                     /locus_tag="ACHBFW_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM9318974.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            280..1017
                     /gene="erm(B)"
                     /locus_tag="ACHBFW_002722"
     CDS             280..1017
                     /gene="erm(B)"
                     /locus_tag="ACHBFW_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM9318975.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1280..2143)
                     /locus_tag="ACHBFW_002723"
     CDS             complement(1280..2143)
                     /locus_tag="ACHBFW_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM9318976.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2145..2417)
                     /locus_tag="ACHBFW_002724"
     CDS             complement(2145..2417)
                     /locus_tag="ACHBFW_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM9318977.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2434..2643)
                     /locus_tag="ACHBFW_002725"
     CDS             complement(2434..2643)
                     /locus_tag="ACHBFW_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM9318978.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2721..3617)
                     /locus_tag="ACHBFW_002726"
     CDS             complement(2721..3617)
                     /locus_tag="ACHBFW_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9318979.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1143 a          652 c          575 g         1347 t
ORIGIN      
        1 aaattttgta taataggaat tgaagttaaa ttagatgcta aaaatttgta attaagaagg
       61 agggattcgt catgttggta ttccaaatgc gtaatgtaga taaaacatct accgttttga
      121 aacagactaa aaacagtgat tacgcagata aataaatacg ttagattaat tcctaccagt
      181 gactaatctt atgacttttt aaacagataa ctaaaattac aaacaaatcg tttaacttct
      241 gtatttattt atagatgtaa tcacttcagg agtgattaca tgaacaaaaa tataaaatat
      301 tctcaaaact ttttaacgag tgaaaaagta ctcaaccaaa taataaaaca attgaattta
      361 aaagaaaccg ataccgttta cgaaattgga acaggtaaag ggcatttaac gacgaaactg
      421 gctaaaataa gtaaacaggt aacgtctatt gaattagaca gtcatctatt caacttatcg
      481 tcagaaaaat taaaactgaa cactcgtgtc actttaattc accaagatat tctacagttt
      541 caattcccta acaaacagag gtataaaatt gttgggaata ttccttacca tttaagcaca
      601 caaattatta aaaaagtggt ttttgaaagc catgcgtctg acatctatct gattgttgaa
      661 gaaggattct acaagcgtac cttggatatt caccgaacac tagggttgct cttgcacact
      721 caagtctcga ttcagcaatt gcttaagctg ccagcggaat gctttcatcc taaaccaaaa
      781 gtaaacagtg tcttaataaa acttacccgc cataccacag atgttccaga taaatattgg
      841 aagctatata cgtactttgt ttcaaaatgg gtcaatcgag aatatcgtca actgtttact
      901 aaaaatcagt ttcatcaagc aatgaaacac gccaaagtaa acaatttaag taccgttact
      961 tatgagcaag tattgtctat ttttaatagt tatctattat ttaacgggag gaaataattc
     1021 tatgagtcgc ttttgtaaat ttggaaagtt acacgttact aaagggaatg tagataaatt
     1081 attaggtata gggcacctct aataactacc aattaattca cctcaaaatg aaattagaaa
     1141 aaaacagcga aatttttaaa tctatttctt atcgatacaa attcctcgta ggcgctcggg
     1201 accccaacat gaggaaatgt ttttataaaa aaagagtagt taccgttttt tggtaactac
     1261 tcttttcctt ttttaaggtt taaatacctg gaagtttagg tgttttgggt attggtggtg
     1321 taggtagctg caagctttcc attttttgtt gaattgcttt aaattcaggg gtttcttggt
     1381 gttgattttg aaccattttt tgctctattc tctccaaagt cggttgaatt tctttccctg
     1441 atactttacg atttaattct ctttctaagg tctctttcgg actaatggaa ggcgtttcta
     1501 agcttgaata gagtttcact ccttctctat tgtaaagcct tatatcgcta aataagcccg
     1561 ttttatgaag ggtttctaaa ttggtcggta agtttttgac aacaatatca tgcgcttgtt
     1621 ttggtgttgc cctagctgtc attggatcat ctgcatacat ggtttcatat cgttcaatcg
     1681 ttcctaagta tgattcaatt ttcggtactg ccatgacgta tatttttgtt tcataacctt
     1741 tagattgaag cattgtggct gtttgaatgg gaacgtctgt tgttcgtcct gttccttcaa
     1801 tcactaaatt atagccttga tcgcttaaac ggctaataag tgcttctgtc atgcgattcg
     1861 aataaggggt aacgtgtttt actacgtctt tttcatagag tttcactaat tcgtcaaaat
     1921 ttgggtgctg ttgtttaaag gtatcattat caatgacaat aacattccct tgtgtttctt
     1981 caaaaattgc tgatcgcaaa ctggttttcc ctgaccctgg ttgcccacca agtaaaaaag
     2041 cggttggcga ttcaaccgct agtctatttt tagttagtcg ttcaacattc ttttctaaac
     2101 gatcttcaaa ttgcttttcg gtgaagtcag taatattcgc catgttaagc cactttctct
     2161 ttattcaaaa tttgttttga atattgattc attgaccgcc aatactcatg aatagcttgt
     2221 aattcttcaa atggttgttg aaataaatca acttcttgtg ccacttctaa ttgatttaaa
     2281 agattcactt ctagtaaacg agtattttta gtatctagtt catgattcaa aacataattt
     2341 aatacccgat tataaacact tgccgataac tcattaatga tttcaatttc aaatgttttt
     2401 tcatacgtaa ctgccatttc attttcactc cttttaaagt ttgtcaggta aatatttccg
     2461 tatatattct tctaaggctt gatccataac ttcttttacg tttccgccat tctttgctgt
     2521 ttcgattttt atgatatggt gcaagtcggc acgaacacga accgtcttat cccccattat
     2581 atcttttttt gcgctgattg gcgtatcatt tcgttttgct ttctgtgcgc ctaaatttcc
     2641 cacgatcgct cacttctttc tatttcttct tactatttat tttatcatca cctatcacaa
     2701 atcacaagtg atttgtgatt ctattctttt tcattttcta attgaatgat tcgctcaatc
     2761 atttcaaaaa atacgttctc atacatagac aaaacttttt tgtcatagcc tttgtgttct
     2821 gtaatgccat ttttagacca agtacttact ttattacttc gcttgataat atttcggaaa
     2881 accaaattat cctctttatg ttctttgtac agttcttcca ggtttgattt tatcgttgcg
     2941 ctgtccgtat caactaaata aggaacaaaa ccaatcatat ctagtccagg gttaaactgt
     3001 tcttgcaaat caatcaaata ggaaatatag ttttgaatgt tgtttgtact ttcttcttct
     3061 gcttgtaaag ggatcataac gtaatcactc gccacgattg cattatttgt ataaacgctt
     3121 ggcgttggta cagtatcaat aataataaga tcatagtcac tttttaaagg tgctaaaaga
     3181 gtagcaagca atctactttc attttcaaac gtccatgagc gagttaattt tggcagtaac
     3241 atcaaatcaa acgtgccagg gatcaagtct aaattatcag tcaaatgaat aatagaagaa
     3301 gccaagtttc catttttcaa gccttcataa aaattgacac gtggcaattc tacctcaaaa
     3361 gtttttgcta agtcttttgt caatgttgcc tgtaagtcct tatcgatcat taaaactttt
     3421 aaattcaatt tgtctgtcaa gtaagcaaac atagtcgata atttggactt tccaacacca
     3481 cctttaaagt aattattcaa gataataatt gcttcatttt tattgtttaa aatacgtctt
     3541 aattcttcaa gtatttttag tttttccttc tccataacta cgccccattc tttttttgta
     3601 tagtaatatt gtatcattaa gaatcacaaa tcacaagtga tttgtgatta atcacttgtg
     3661 atttgtgata agtgatttgt gattaatata taaaagccct ctttaaaggg cttttat
//
LOCUS       DAYYME010000194         3699 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000194 DAYYME010000000
VERSION     DAYYME010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.197"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            38..1231
                     /locus_tag="ACHBFW_002727"
     CDS             38..1231
                     /locus_tag="ACHBFW_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9318980.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            1224..2951
                     /locus_tag="ACHBFW_002728"
     CDS             1224..2951
                     /locus_tag="ACHBFW_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFM9318981.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            3345..3542
                     /locus_tag="ACHBFW_002729"
     CDS             3345..3542
                     /locus_tag="ACHBFW_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM9318982.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            3544..>3699
                     /locus_tag="ACHBFW_002730"
     CDS             3544..>3699
                     /locus_tag="ACHBFW_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM9318983.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLIN"
BASE COUNT         1275 a          597 c          810 g         1017 t
ORIGIN      
        1 aaagaaaaat gaatttacat aaaaaaggag aatttgtttg gtaaaagtag aagtaaaaca
       61 tttaacaaag atatttggta aaaagacaca agccgcttta gatatgatga atgatcacca
      121 gccgaaaacg gagattttaa agaaaaccgg tgcaacggtt ggtgtgtatg acgtgaattt
      181 tgacgtaaaa gaaggagaaa tcttcgtgat catggggctt tccgggagtg ggaaatccac
      241 attgatccgt ctattgaatc gtctgattga gccaacttcg ggcagtatct atattgatgg
      301 agaagatgta tcgaagttaa gcaaagaaga attgcgagaa gtccgtcgtc ataaaatcaa
      361 tatggttttc caaaacttcg gtctgtttcc acatcgaacg atcttggaaa atacagaata
      421 cgggttagaa gtccgtggtg tacctaaaga agaacgacaa gaaaaagcag aaaaagcatt
      481 agaaaattct agtttgcttt catttaaaga ccaatatcct agtcagttat ctggtgggat
      541 gcaacaacgg gttggcctag ccagagcttt agcaaatgat cctgagatct tgctgatgga
      601 cgaagcgttc tcggctcttg atccgctgat ccggcgagaa atgcaagacg aattactgga
      661 cctgcaagcc aatgttcaaa aaacgattat tttcattacc catgatttga atgaagcttt
      721 acggattggg gacagaatcg ccttgatgaa agacggcgaa atcatgcaga tcggaacagg
      781 tgaagaaata ttgaccaatc cagcaaatga ttatgtacgt gaatttgtgg aagaagtcga
      841 tcgttccaaa gtgcttacgg ctcaaaacat catggtacct gcattgacga cgaatatcga
      901 atctgatgga ccaaacgttg cattgacaag gatgcgtaat gaagaagtca gcatgctgat
      961 ggctgttgac agaaagagac acctgaaagg gatcatcact gcagatcaag cgttagaagc
     1021 taggaagcaa aaacgtccat tgatcgactt cctagatgaa aatgtgacgg tgattgggaa
     1081 agacatggtg gtaagtgata ttttcaatat tatttatgac tcaccgactc cacttgcagt
     1141 tgtagaggat ggaaaattaa aaggtgtcgt catccgaggt agtgtgattg aagcattagc
     1201 agaaacaagc gaggtgagtg aacatgaata atctattgaa ctatcaatta ccagtggcaa
     1261 actgggtaga aaatatcaca gaatggttta cgacaacttt ttctggccta ttttcatttt
     1321 tacaaacgat tggacaggca gtgatgtcgg ggattacaaa tttactgtta gtgattccag
     1381 ctcctttgtt tatcttgtta ttgacggtag cagctttctt tatttctaag aaacgtccag
     1441 gacttacgtt gttcacatta atcggtctat ggtttattta taaccaaggt ctatggaatg
     1501 atttgatgaa tactgtcaca cttgttcttt tatctagtgt gatctcgatc atcattgggg
     1561 taccattagg gatcttgatg gcaaaaagca gtaaagccca aagtattatc aaaccgatcc
     1621 ttgactttat gcagacaatg ccaggattcg tttatttgat tccagcagtt gcatttttcg
     1681 gtatcgggat ggttccaggg gtatttgcct cagtcatctt tgctctgcca ccaaccgttc
     1741 gttttacgaa tttagggatt cgacaagttc ctaaagaatt agtagaagct tctgattcat
     1801 tcgggagcac aagtcgtcaa aaactattca aactagaatt gcctttagct aaaagtacga
     1861 tcatggcagg gatcaatcaa acaacaatgc tgtcactttc aatggtcgtt attgcctcta
     1921 tgatcggtgc acccggctta ggacgcggcg tattatcagc attgcaaaga gcacaagtcg
     1981 ggaatggatt tgtcaacggg gttgcattag ttatcttagc aatcatcatt gaccgtttta
     2041 cacagcattt gaaccagcca aacaataaaa aagctgcagg agcaacaaca cagaaaagta
     2101 aaaaacgtca aggactgatc attggtgctg tagtagtagt gatcttaggt gcgattggta
     2161 tagggagttt ttcatctgcc aaagaaacaa aacgtatcaa cttatcttat gttgaatggg
     2221 atacagaagt tgcttcaaca aatgtagtcg gtgaagtatt gaaacaaatg ggctatgatg
     2281 taacgatgac gccattagat aattcaatca tgtggaaatc tgtttcaaat ggcgagtcag
     2341 atgccatggt ttcagcttgg ctacctaaaa ctcatggttc acaatatgca caatataaag
     2401 atcaagtcga agacctaggt gccaatttga caggagcaaa agttggttta gcagtgccag
     2461 cctacatgga tgtaaattcg atcgatgagt taaccgatca ggcaggtaag aaaatcatag
     2521 gaatcgagcc aggagctggt gtagttacgg ctgctgaaaa cacgatccaa aaatatgaca
     2581 acttgaaaga ctggaaagta gagacatctt cttcaggagc aatgaccgtt gcactaggtc
     2641 aagcgatcaa aaaacacgaa ccaattgtag ttacaggatg gacacctcat tggatgtttg
     2701 ccaaatatga tctgaaatac ttagaagatc ctgaaaacgg aatgggaagc gaagagcaga
     2761 tccatacgat ggttcgaaaa ggattgaaag aagaccagcc agaagcttac aaagtattag
     2821 acaatttcca ttggtcagaa aaagatatgg aaaaagttat gttggaaatc aacaatggaa
     2881 aagatccaca gcaagcagca aaagactgga tcaaagagaa ccaagaatta gtcgaaagct
     2941 ggaaaaaata aaaagacaga cacgaaataa atggagcttc caaaattgga tcaaatatcc
     3001 aaatttggaa gctccatgtt cgtctctatc ttttttgtct ataaaaaaag agaatgcgta
     3061 gctcatcttc gaaacgttca aagacgccga caaactatta tgaatttatg ggtgcgagtc
     3121 aaagagatcg atcgtaggta tgaaagacta atgtgattaa agtttattat gtacgaagtg
     3181 cattctcaaa aaatattaaa gaatatacat agtttatata cttaaaagtg attaaattct
     3241 tattcaggaa ttattttgct acgtttcaag aaggaattgt tttttgaaat ttaatgaatg
     3301 ttagaataat agtctaaaaa cattaattta ggggtgatta gattatgaaa catttaaaaa
     3361 ttttgtctat taaagagaca caacttatct atgggggtac cactcatagt ggaaaatatt
     3421 atggaaatgg agtgtattgc actaaaaata aatgtacggt cgattgggcc aaggcaacta
     3481 cttgtattgc aggaatgtct ataggtggtt ttttaggtgg agcaattcca gggaagtgct
     3541 aaaatgaaaa aaaatgctaa gcaaattgtt catgaattat ataatgatat atctataagt
     3601 aaagatccta aatattctga tattcttgag gttttacaaa aggtatattt aaaattagaa
     3661 aaacaaaaat atgaattaga tcccagtcct ttaataaat
//
LOCUS       DAYYME010000195         3691 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000195 DAYYME010000000
VERSION     DAYYME010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.198"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..878)
                     /locus_tag="ACHBFW_002731"
     CDS             complement(27..878)
                     /locus_tag="ACHBFW_002731"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9318984.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            1001..1468
                     /locus_tag="ACHBFW_002732"
     CDS             1001..1468
                     /locus_tag="ACHBFW_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFM9318985.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(1731..2213)
                     /locus_tag="ACHBFW_002733"
     CDS             complement(1731..2213)
                     /locus_tag="ACHBFW_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM9318986.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(2313..3176)
                     /locus_tag="ACHBFW_002734"
     CDS             complement(2313..3176)
                     /locus_tag="ACHBFW_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM9318987.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         1087 a          788 c          578 g         1238 t
ORIGIN      
        1 cggctttttc acaacctctt tatacattat tcctgatcca atatttcttt taatgctgct
       61 tcaagagaag ttaatggttt tcccaatact ttttctaaat catcagagga aatagctaac
      121 tgatcctttt taatatcatg ttggatcgct aaaaacatgt cagctgcttc ttgagggaat
      181 ccggcttctt ttagattttc gacaaacgct gcatcacttg cttcaataat ttctaacttt
      241 ttacctgtcg cacgctctaa tgccttggct aattcttcat agtcagttag attgccggat
      301 aattctaaga tttctggaga gtcactttct actagtactt ttgctgcagc ttctgcatat
      361 tcacgtttca atgcccaacc tgtttttcca tttcctgcag cataaacgaa ttgaccagtt
      421 tttaaagcct gcccaatgat aggtaattca ttctctacat accaattatt ccgtaaaaat
      481 gtatgggaaa tacctgaatc cttaatcatt ttttcagtga attggtgatc tggcgctaat
      541 acactgatcg agtgatcggc atctgcaaaa cttgtataag cgatataaga taccccagct
      601 gcttgtgctg ccttcactac attcttatgt tcggcttgac gatttcctgg aacgccagag
      661 acaaaaagca atcgatctat tcctttcaac gcttctttca ttgagtctaa atcatcataa
      721 tcaccaatac gtatattaaa tccagcttct ttcagtgctt ctcctttttc ttcactacgt
      781 actagtacat agatttcatt ttgtggaacc atctttttta agaattccag cgcataacca
      841 ccaaaacctc cagttgctcc tgttactaaa taatgcatat ttgccactcc ctattctttt
      901 tatttgttaa gatttacagc tgtacattta taaattacac ctttatcata aagagtgtgg
      961 taagataagt caagcaaaaa aaatgaaagg atgacaactg atgaagtatt ctctgcaatt
     1021 tagtgatgca atccatgtac ttgcctatat tgaaatgttt caagataccc gatcactttc
     1081 tagtgagatg attgccagta gtatcgaaac aaatgcagca aatgtacgta aaatcatgag
     1141 tcagctcaaa aaatccggtt tgatctttac aaaaaccgga aaaccgcagc cttcccttgc
     1201 caaaagtcca gatcagattt ctttactaga tgtctatcgc agcatcgaag gtaatacaca
     1261 gcttatccaa gtcgatccaa aaacaaatcc aaattgcgtc gttggtgcga atatccaaga
     1321 agctttagaa gaaagctacg ctcggctgca gaaaaaggca gaagaagaaa tggctaagat
     1381 cacacttgat agtctgatac ataaaatagc cgttttggaa aaagaaaaaa gaccagaaaa
     1441 cacaggcctc gtagagagat ttttataatt ctctctactg tgcctgcatt tttctgatcg
     1501 gaatcgtcta cataaattct tttattctcc gggggacaaa agccttgtca tgcccctttt
     1561 tcctctctaa tgctcaggtc aaagcgctga agtcatgacg tcttgataaa cggtgttcaa
     1621 gtagctgacc ctcggcatta gttctgaaaa atgcaaaata tggaaagcta tttcccattt
     1681 ttcctctcta atgctcaggt caaagcgcta ctttcacgac ctctatatta tcatggttta
     1741 ttgactaccg gtatccagat ttctcccata tatttcccgt tggtaactcc catttcgact
     1801 acagcatttg gacccccaac ataggcgtaa tcttctactt ctcttaaaac atgcccaaag
     1861 gccgtattcg tcaaatcatt tcttaattct tcaggcgatt ctgctaaagc gattacaaca
     1921 agatacgttc cttttgggaa tgggatgatt cgggtagtgt ttggaacatc ctgactcgtt
     1981 tgaacaccga catagtacat cattttcccg ttatagggtt cgttgactgt aaagaaataa
     2041 tgattttcag cagcttgttt caatgcatct attgttccat taccgataac tttttgctca
     2101 aatgcttgtt tctctgcagc caaaccgaca aaatccgtat tgctactctt caattccaca
     2161 ccaatacccg tcaccgtaaa atctggtttt tcttcgatcc tatactttgc catttctccc
     2221 accctttcta ttatattaag ttcatcttac ataaaattaa agggaaatga cacgattacg
     2281 ctcttttttc tagggatatt tttatttttt aactattttt ttgctttcac tgcaatccaa
     2341 atctcactgt aaacattact gaaatcctct ccccattttg taaaagagat ctcgggagct
     2401 tcgactaact cataatctga agaaggaagc cattctgcat agattttccc ccaagtatct
     2461 tgtaaagttt ggggaaatgg ccctttatta ggaaaaatgg cccaagtgtg ttcttctata
     2521 gcaagcattt ctaaatcagc gtacggattt tcttttgtcg tagcaaagcc aatcatgtgc
     2581 atcaaactgc ccttttcttc caagcgggct ccatcaaatc cataagaaac gttgattact
     2641 tgatttggat ataaatctcc taacttatgc atctcttccc tttgagtctc cgtgatcgtc
     2701 tgcgctaact tttgtatttc ttgattgact ccttcaaatt tgacaggaac ttttttcgcc
     2761 acacctgcca cataaaactg ttctttcttt tcgatacgaa attccatcga ttttcctcct
     2821 ctgacatcaa taaaaaatgt caagcgtgga aaagattttt gtatgccgta tctgctgatt
     2881 tctgaaggca gatatcctga ccaatcacga aaagctcttg aaaaaccttc gactgtttca
     2941 tatccatatc taaacgccgt ttctgtcact cttgatccat ctaggagatc gcgattagcc
     3001 aaagccaatc tacggttttt gatatagtcc attagcgaca tccctgcaat aaacgtaaag
     3061 atgcgtttta attgatattc agagattcct gtattttcgg ctattatttt ataggaaagt
     3121 ccttctgtca aatgttcttc gatgtaatcg atcaattggt tcagatcttt aagcatattt
     3181 atcttcctcc ttgcttgcct ttttatcata ttatcaaaga aagtcaatta cttgatgaat
     3241 caggaaccaa tttagtcaga tgtagaaaat gttgtatgga tctaaagaaa attctcaagc
     3301 agcgtttcgt aacggataat tggtattatc tgcctatcta tgttttacct ttagaaaaat
     3361 aaattttggg gaagacacat tctaagtaaa caaagcggta aaaatatctt ttattccctt
     3421 agttaatccg cttactacct actgtacaaa gcaaattgtt gttcaaactg ctgatcagaa
     3481 cgctgcttgg atttttcttt gcgatacatc atacctcttt tatcaattgg aacaattatt
     3541 tatttctaac aaaatcactt catttttgtc agataaataa aaaacaaaaa aagttccatc
     3601 tttaaaacac gctattcttt tactttttct cacattctct aagaaaagaa aaggagaatg
     3661 aatttttgac aattcgagca aaagtgatta t
//
LOCUS       DAYYME010000196         3671 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000196 DAYYME010000000
VERSION     DAYYME010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.199"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACHBFW_002735"
     CDS             64..972
                     /locus_tag="ACHBFW_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM9318988.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..1592
                     /locus_tag="ACHBFW_002736"
     CDS             972..1592
                     /locus_tag="ACHBFW_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318989.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            1596..2045
                     /locus_tag="ACHBFW_002737"
     CDS             1596..2045
                     /locus_tag="ACHBFW_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318990.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            2045..2539
                     /locus_tag="ACHBFW_002738"
     CDS             2045..2539
                     /locus_tag="ACHBFW_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318991.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            2553..2984
                     /locus_tag="ACHBFW_002739"
     CDS             2553..2984
                     /locus_tag="ACHBFW_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318992.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            2986..3123
                     /locus_tag="ACHBFW_002740"
     CDS             2986..3123
                     /locus_tag="ACHBFW_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM9318993.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            3160..3453
                     /locus_tag="ACHBFW_002741"
     CDS             3160..3453
                     /locus_tag="ACHBFW_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318994.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            3450..>3671
                     /locus_tag="ACHBFW_002742"
     CDS             3450..>3671
                     /locus_tag="ACHBFW_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM9318995.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT         1320 a          546 c          813 g          992 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat tgcggatatg
      481 acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag agaaggacaa
      601 gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac gattgatcca
      661 ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg agttcgtcat
      721 gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa attgcgagca
      781 acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca ggatgatctc
      841 ttttcagata atcatgaagt cgaaaaagtt ggaactgtag cattcccgat tgaagaagga
      901 gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct tgagaaagtt
      961 ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa acgggtgtac
     1021 aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa gaaataatag
     1081 caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc attactaaag
     1141 aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa gaagatgacg
     1201 gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt gactacgtgt
     1261 tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta ttgacgattg
     1321 atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg ctggagttaa
     1381 aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact attgatgaag
     1441 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgtcaatcag tcatatgtag
     1501 atcaaaaatt ccaagaagct gtaaatcaag cagaaaatta cacaaacgaa agaattccaa
     1561 attttacttt tgaaaaaatt ggggaggtat agagaatgac agatatcgta aaaataaaac
     1621 aaagcaatgt acaggtttat ccacaaactc attggaatgc tatagaaggc aaaccaacaa
     1681 cggtgaaggg agataaaggc gatcctggcc aagcagcaac aattaccata ggaaccgttt
     1741 ctagtggttc aaccgcttct gttacgaatg taggaacatc atctgcagct agatttaatt
     1801 ttgttttacc aaagggagat aaaggtgatc ctggaataaa tgccacaact acagctgtag
     1861 ctacaactac agcaaatggg ttgatgtcgt ccacagataa aactaagttg gatggaattg
     1921 cagctggagc acagaaaaat ccaggtaatg ctacaactac gacagcaggt ttaatgtcag
     1981 caaccgataa agtgaaactt gatggattag cgaatattac atttgagaaa gtggggacgg
     2041 tttaatggct gatattgttc aattaaaaga agacggagtt gctaaatact taaaaacgca
     2101 tgtagaagct attgatggta aagaagcatt ggtgcagaca gatggagacc aagccattgc
     2161 aggacataag aatttttcag gttctgtaac aatcaataat aaacgtgttt tgacgactga
     2221 tgataacaga tatgaagtgg taaatctggt tgtcactaac ggtaatacag ggacagcaaa
     2281 gctttatcgt gaaggaaaaa cagtttctat atattttgtg gctttaaacg gaaaaagtag
     2341 tggcgggaat gattcggtta ttttaactgt tccagaaggc tatcggacac caattagttt
     2401 tgaacaactg gttgggtcaa tagaccgttc tactttgaac agtgctcaac tatctattgg
     2461 cgcagacgga gccattaaat ggagaagaaa ttcaagttat ggatcagctt attcatttgt
     2521 tatcacttat tcaatttaag ggaggaaatc taatgaaagt agtttacaaa tcaatcaagc
     2581 cttacggatt cgagcaaatc attttgaaca atcaagaaaa tatccctgaa aactgtacag
     2641 agattaaacc accagttcct aactggagac caagatttga ttttgataaa aaacagtggg
     2701 ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt tgatagcgta gatgaattgg
     2761 ctaatattaa ggcattatac gaaacgctga aggcagaaaa tgatgaattg aaacagctaa
     2821 attctaaagc aatgctaaac aatgtagcaa tcaagcaaga aaatgtctta ttgaaagaaa
     2881 agtcagaaag tttagcgcag ttgaattcaa aaacgatgct tgcttctgta caaaatacca
     2941 aggaaatcga agaaatcaaa aaacaattac aaggtgggaa gtaacatgta ttcatatgaa
     3001 gatatcaaac taatgtatga ctggggctgt ttcactaacg aacaagtaat ggttttcgtt
     3061 ccattatgca ttacagacga agaagcagat aaaatcatta ataaagataa gagcgcatct
     3121 taattgatgc gtttttattt aaggtaaagg agttgtcaca tgattaattt aggggaatgg
     3181 ggaatgatag caggatcaat aaccgctatc gtttctttga ttttattagt aattagaccg
     3241 attgctgcat ctttctcgaa aattactgag actctttcaa aagtaagcca caatttagat
     3301 ttgctgacta aagatttaga atcgagcaaa tcagatcgat tgatgattca tgaagaacta
     3361 aagaaacacg atgaaagatt agatacacat acagaaaaat tggtggaaca cacgcaacaa
     3421 atcaaaactt tgtttaggga gagaagaaaa tgaataataa aacgttcgaa gtactaaaat
     3481 ggttcgcact ggtaattatt cccgcactag ctactttcgt ggggttagtt ggtaaagcgc
     3541 tcaattggca gtacacagat atctgtgttg tcatcattac tggttttggc gcgtttttag
     3601 ggagtgtgtt gggtgtatca aatcgaacct acaaaatgtt ctcggctgaa agcgaagaag
     3661 gaggaaacaa a
//
LOCUS       DAYYME010000197         3613 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000197 DAYYME010000000
VERSION     DAYYME010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.200"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..327
                     /locus_tag="ACHBFW_002743"
     CDS             94..327
                     /locus_tag="ACHBFW_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFM9318996.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            324..731
                     /locus_tag="ACHBFW_002744"
     CDS             324..731
                     /locus_tag="ACHBFW_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM9318997.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(1868..2386)
                     /locus_tag="ACHBFW_002745"
     CDS             complement(1868..2386)
                     /locus_tag="ACHBFW_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFM9318998.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            2753..3001
                     /locus_tag="ACHBFW_002746"
     CDS             2753..3001
                     /locus_tag="ACHBFW_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9318999.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
     gene            3504..>3613
                     /locus_tag="ACHBFW_002747"
     CDS             3504..>3613
                     /locus_tag="ACHBFW_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9319000.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT         1235 a          614 c          554 g         1210 t
ORIGIN      
        1 tttctataga gttagacatg agagggacta tgcttgattt aaaatcaaca agcgtatata
       61 ctgtatatac aaagaaaaag gagggagaag actatggcaa ttaaagaaag aaaatttagg
      121 gaagtgggga actcagtagt attaactatt tcaaaggagt ttcttaaaga aaatgggtta
      181 aatcccggag atacggtttt tttagatgaa gaaaaacttc gagcggcaat tacaaaaaaa
      241 caagccttat ctgatgaaat tgatttagca atatctcaag cattaactga atatgatact
      301 acttttagag agcttgtcga taaatgagta ttcattacct agaagccaaa gatattataa
      361 aaatgaatgt aatccaaata aaaaaatact ctcctaatga accccttggt gtaaaggatg
      421 cgaatgcttt agaaatgtgt gtagggcaac ttcgagcctc agcttttggt gaagaggttt
      481 atccttcgat ttatgaaaaa gcagctatcc tacttataca attgataaag aaacatcctt
      541 ttcacaatgc aaataaacgt actgctttct tagctacttt tgtctttttg aaaatcaatg
      601 gacagctttt agctattgat caaaaagatg ctgtaaattt agcggtatat attgctacgt
      661 atgataagga tttcgatcaa ctaaaatatg aagtgatcaa cactataaag acacatacgc
      721 agcccattta aggagttact gtatgtgtct ttatagggtt attagctgct atagcgaaat
      781 aattgaagta aattacaaat agctataatt caaatccaac tatatagaaa acgtaaattt
      841 tctatatagt tggattaaga taacgactat ttattttatc atatttttac tataccctat
      901 acttttactt ggtatattta tgaataatcg caaatttacc cctatactac tagtttgtaa
      961 gttagattaa taaaaatact aaagttattt gttcttttat taaatacacc tacaacttat
     1021 aaattgatgg tatttagata acacgaaaga ataaaaataa cagctgatag taaagtttgt
     1081 tgacgattgc tactcaatcg aaagtgagtt agtgcgtatg aacttgcttc tgaaaactta
     1141 tgaaatgaaa gaccttttac atcttgcaga aatcgttact gggattaaac taagcaataa
     1201 aaactaatgt tcccctcatt ctttgaactt ctactatttg atttatttat tctgataaat
     1261 gacgatttca ccttcaggat cttcaaatcc cgtatagaat ttatttaaga tctcttcagt
     1321 atcagtaata ggataggcga taggccacat accctttgat ggttagaata aactgctcaa
     1381 aatctgtatc gtatgttggc tttagcatat taacaaaggc tatctccaaa ttataaacca
     1441 taagcgttga atcgttctta acaatttcaa ttcttcctaa cccaaagtca gaagatataa
     1501 ttaccggact tgccttaccc ttcatgtggg ttgttgagat tccatattta aagggaattg
     1561 tcggtactat cggcggtctg aaaaacatcc caaaaataaa aaaatctttc ccctgctagt
     1621 ctcctaagtc cctttgtagc ggatatggca ccataatctc attttatcga agtgagtcat
     1681 ggtttatctg ttggccaacc aaaattgata caatctaacg atgcaccctc tacggcattc
     1741 taggggagtg cacttgcatt cacccaataa aaaccggcag ttagcaaaga acttatgtag
     1801 tcgtatgttc ttactgttaa catacaagag gtctcctttc cacaattgct tagcagtaaa
     1861 cgggaattta caaagctaat ctcttccatt ttcttatctt tgttctaaaa gtcccaaatg
     1921 gagcaactgt atttacatga ataaatttat atacttccca tacagccgtt ttggttgcct
     1981 cgtctgccca ttttcttttg tgtgcagtga atagatcttc atcactcatt gaatcaatca
     2041 tcatgttaat atcatcaata ttacgattga gaatttcctc taattctttc aatggtagat
     2101 gtgcataggt atttgtaaac cacttatata actctcctaa ctgattccat ttaaattgat
     2161 cagaaggcgt tttaacagat aatccatttc tttcatcatt ttcccatttt agaattaaat
     2221 tcgtccaacc aacttgatat gctaaatttt cagcaggtgt tctatccact tcctcaactc
     2281 ttttatcctt caattcttca ggaatagttt caaattctaa aatatattta gtataagtcc
     2341 tatttatttc tttttttaat tcttctttat ttttatatgt tctcattata ttacctccat
     2401 aaattcctaa ttgtctcctt taattcatct ataatttcat ccacgatatt ttaatccgtt
     2461 ctacaatgca tatctgatat gcattgactg tctaactcat atgtgaatga tttataaaat
     2521 atcccaacgt ctcatctaaa gaaatgttga tcgtcagcaa ggagctaaaa aatacgaata
     2581 tatttctttt gtagtaaaat cggactggtt tgcatatcat tttcttcgta aatatcaaaa
     2641 tcatgttaac tactatcaat tactaataaa aactaccact ttaagcgtta agaactgtta
     2701 agtttagtta agtgaggata gttcacaaaa tccattgtta aacttcacga aagtggtaaa
     2761 actcaaaatg ctttatatag gattacagca tcgctctttc ttcgattgcg cgttgggtaa
     2821 aaaatattat gaagtaaaaa ttgacgatga cacgttcctt acggctcatc agatgaaaca
     2881 gctacaaaaa cgaaatgatc aactcgaaga aaatttaatt ctaaaaaagc gattgccata
     2941 ttcacgcctc gctcgacgaa ctattaaagg tcaagattta cctgaagatc atccttttta
     3001 ggaaattgaa gctttttatg ttgcacaatg catttataat actacaatga tgttttcacc
     3061 agatattctt ctagggtgcg gtgtaatgaa acagatacac ttgcaaaaga aaattcaccg
     3121 cgaatttact aaaattatga ataattatgt taatcttccg aaattttttt ccaaaattaa
     3181 gggatcaagc tggtgttata gattgtttaa ggttggcaaa aaaataactt ctttttaaac
     3241 aattatagaa aaccgatact ctgttaagtt ggttgacacg gtgtcgattt ttatgacttt
     3301 cttattctga aacttcacta acggacagta cctaaaaagt ctatagtatg gacgtttacg
     3361 agtactattt tatcaaaaat gctgctcatt tcaacttata tgttttagtg aactaccccc
     3421 acttagcttg gataggctac aattaactag acagaaaaat taaggtgtgt agactagaag
     3481 aaaacatacc aggaggaatt tttatgtcta agagaacacg aagaactttt tcacaagaat
     3541 tcaagcaaca aatcgtcaat ctttacttag ctggaaagcc acgtgtagaa atcattcgag
     3601 aatatgaact aac
//
LOCUS       DAYYME010000198         3608 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000198 DAYYME010000000
VERSION     DAYYME010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.201"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACHBFW_002748"
     CDS             complement(<1..2232)
                     /locus_tag="ACHBFW_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9319001.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACHBFW_002749"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACHBFW_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT         1034 a          786 c          557 g         1231 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaataac aaactaacta tccatcaata acgtgtttta ttttttaaaa tttctataaa
     3541 aaaacaatca ttcttaaaaa gaatatataa aaataacaat aacctttgtg atttttcttg
     3601 tttgaaga
//
LOCUS       DAYYME010000199         3567 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000199 DAYYME010000000
VERSION     DAYYME010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.202"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..1882
                     /gene="yfcC"
                     /locus_tag="ACHBFW_002750"
     CDS             143..1882
                     /gene="yfcC"
                     /locus_tag="ACHBFW_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFM9319002.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2039..3223
                     /gene="iadA"
                     /locus_tag="ACHBFW_002751"
     CDS             2039..3223
                     /gene="iadA"
                     /locus_tag="ACHBFW_002751"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFM9319003.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3334..3537
                     /locus_tag="ACHBFW_002752"
     CDS             3334..3537
                     /locus_tag="ACHBFW_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM9319004.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
BASE COUNT         1142 a          593 c          826 g         1006 t
ORIGIN      
        1 cataggagaa aaaaaacaaa atagaaaaaa gtgattttct aataaattat tcatgtggaa
       61 atgtcatttt tactgtataa ttcatttgat tatccatttt ttattcatca aaattactga
      121 aaaaagaaag gaagaaataa atttgaagaa aacaaattct tcaggaaaac cgaaacgcga
      181 cttcactaaa ttatcgacac cccatacgta tgtcattatt tttggtgtag tcatctttgc
      241 atggatactg acttttgtcg ttccagcagg aaagttcagc acgcaagata ttgaatacaa
      301 agatgcaaat ggcgaaacaa gtactcgtac tgttttacgt caagattcct tccgttatgc
      361 ttacgagctg gataagtcgt atgtgtttga tcaattagaa gaactgcaag atcatccagc
      421 agaacgtgaa aaactggatg ttccggaaaa aggattggaa aaagtcattg ctgacggaga
      481 aaaaaatctt acgcaggaaa aactagatga aatctccttg acagatgatg tgctttacga
      541 tcagtacggc gaaaatatct atgatacttc taaaaaattg cataaaacag ccaaaatatg
      601 gggaacagat gattttggcg gctttggttt cttaaatttt gtttttgaag gactagtatc
      661 tggagataag tatggatctg ctgtagggat cgctgcattg atccttgttg ttggaggtgc
      721 ttttggtatt attatgcgta ccggagcgat cgacgcaggt atctatgcgt ttatcagtaa
      781 aactaaggga ctggagcgtt tagctttacc gctattattc tttgcttttt cattcggtgg
      841 cgctacattc gggatggcag aagaggtaat tccattctca atggtgatgg ttccatttgt
      901 cattgcgtta ggatatgact cgatcgtagc agtaaccgtg acttatgtcg catctcaagt
      961 cggaaatgcg acctcttgga tgagcccgtt tagcgtagcg gtggctcaag ggattgctgg
     1021 aatccccgtg ttgtctggtg cgacattccg attgattatg tgggtagttg ttactgcatt
     1081 agccgctggt tatatgatga tatatgcaga aaaaatccgt aaaaaaccag aaagttcgtt
     1141 gacttacaaa tcagatgatt atttccgttc tcatatcaaa aagacatctg atgaaaataa
     1201 aaaattcatg cttgggcata aattgatctt attagagatg ttagttgtcc ttgtctggat
     1261 cgtttggggc gtgactcaaa aaggctatta cattccagaa atcgcttctc aattctttgt
     1321 gatgggatta gttgctggaa tcattgctgt tcttttcaaa ttagatggaa tgaaagtcaa
     1381 tgacatcgct tcttctttcc aatctggtgc agctgattta gctggtacag caatcgtagt
     1441 aggtatggcc aaagggattc ttcttgtctt gggaggatct gatgcatctg ttccttcagc
     1501 tttgaatacg attttgcatg ggatcgggac ggcgttgacc ggtgttccag ctgtcattgg
     1561 tgcttgggcg atgtacatct tccaaagttt gttcaacttg gttgttacat caaattcagg
     1621 acaagcagct ttgactatgc caatcatggc tccactggca gatttagtag gtgtatccag
     1681 acaagtagca gttcttgctt atcagcttgg ttcaggtttt atggacgctt ttacacccgt
     1741 atcagccagc ttgattggtg tgctaggtgt tgcacggatc gaatgggcaa aatgggcaag
     1801 attccaaatc aagatgcaag gattcctgtt tgttttagga actattttca tcatgattgc
     1861 cattgcaata ggtctgcagt agtactggag acctgtaaat aaaaaagtaa tccaaacagt
     1921 agataaggac tccaagaacc gtcattttac ggtaaaatca aaggagtaaa gatcctatcg
     1981 tttggattat tttttaagat cctatcatca gttatactta aagaaagagg aaaaatgtat
     2041 gaaaatcatt cgacaaattg aagtgtttgc accagattac ttaggaaaaa tggatgtact
     2101 gattgccggt gggaagattt tggctgtaga agaacagcta gaaggcggct acgaaggagt
     2161 ggaagtagaa gaactttcag gtgagggaaa agtactaacc ccgggtttca ttgactgtca
     2221 tttccatatt ttaggtggag gaggagaagg cggttatcaa aatcgaacgc cagaagtcac
     2281 attgagccaa ttgactacag ctggtgtaac gacagttgtc ggttgtttgg gaacagacgg
     2341 agaaggtaga gatatgactg cgttgatcag caaagcgaaa ggtctggaag cagaaggaat
     2401 ctccacttat gtatacgaag gttcgtaccg attgccggta aaaacagtaa cagattctat
     2461 catcaaagac tttttgacaa ttgataaaat tatcggaatc ggagaaatcg cagtatccga
     2521 tcaccgttct tctcagccaa gttttgaaga gttcgctcgt gcagtagcag atgctcgtgt
     2581 aggaggcatg ttatccggaa aagctgggat catcaatgtc cacttaggcg gaggaaaaag
     2641 aaagctagag ctattgacac gaatcgtaga agaaacagaa attccgatca cacaattttt
     2701 gcctactcat gcaaatcgca caccagaatt atttgaagct tgcgtggcat ttgccaaacg
     2761 tggtggcacg attgacttca ctgctagcga agatcctgat ttttgggaga agacagatgg
     2821 tgaagttcgc ttcagcaaag cactgaaacg attgatcgag gaagatgttt ctttagataa
     2881 tttcacgatg acctcagatg gacaaggaag cttgccttat tttgatgaaa acaatcattt
     2941 cctagggtta ggagtaggaa gtgccaaggc attgctagta gggatcaaag aagctgtgca
     3001 aaaagaaagt atccctttgg aaatcgcctt acgagctata accagtaatc cagcacgtat
     3061 attgaaattg gataaaaaag gaaaaatcga aattggtgct gatgcagatc tgtgtatctt
     3121 ggataaagaa acgcttgata ttgatacagt tatcgctaaa ggagaaataa tggtccaaga
     3181 aaaagaagtc aaagtatggg ggactttcga aaaaagtttt taatcaacag ctctaactat
     3241 cttttttaga aaacgagcgt tctgtttgtc tgtatctgtt gttgatgtta ccctgaaggt
     3301 agatcaataa acaaactaag aggaggaagc acaatggcag atttaaaagg acgttctaaa
     3361 gatatgaaag ataaagtagt cggtgaagga aaagaagcat acggcaaagc aacagacgac
     3421 aagagcaaag aaacagaagg gaaagcacaa tcactaggtg ctgacatcaa agaaaaagct
     3481 cgaaatttag gcgacgaagt caaagacaag ttttctcgaa aagacgaaaa aaaataaaaa
     3541 ccacttatta ctaattgaat caagcaa
//
LOCUS       DAYYME010000200         3551 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000200 DAYYME010000000
VERSION     DAYYME010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.203"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            65..1396
                     /locus_tag="ACHBFW_002753"
     CDS             65..1396
                     /locus_tag="ACHBFW_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFM9319005.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            1362..1712
                     /locus_tag="ACHBFW_002754"
     CDS             1362..1712
                     /locus_tag="ACHBFW_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319006.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1804..2223)
                     /locus_tag="ACHBFW_002755"
     CDS             complement(1804..2223)
                     /locus_tag="ACHBFW_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFM9319007.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(2224..2568)
                     /locus_tag="ACHBFW_002756"
     CDS             complement(2224..2568)
                     /locus_tag="ACHBFW_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFM9319008.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            2918..3514
                     /locus_tag="ACHBFW_002757"
     CDS             2918..3514
                     /locus_tag="ACHBFW_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM9319009.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          934 a          645 c          646 g         1326 t
ORIGIN      
        1 atgatagttg tccaaatctt tcatttcaag ttatagttaa agaaaatgaa aaagaaggtg
       61 tgtaatggaa aacgaaactg acgttattta tattcatcct caaaaacgaa tcgtttcaca
      121 gaaaagaaag tatttttatc ttggttttac aggtgttttt tttcttttta ttggcttgct
      181 ttctaatact cctactgata attggagtgg attgctaact attcttacct cgccaagcaa
      241 tcttttgaca gattattttg ctttaggtgt tttcggaagc gcattcataa atgtagggat
      301 tttgacctta ctttctgtgc ttttagctta tcggcacaag gtgattttaa acggtccctt
      361 atttgcttcg attctaacag ttaccggttt ctcttttttt ggcaaaaacc tttataattc
      421 tatctcaatc attttaggtg tgtatctata cgctgtattt gtcaataaac ctttttctca
      481 gtacatcatg atcggactgt ttggtagcgt attatcaccg gtcgttagtt atatcacttt
      541 tggtatgcgt tttcctttac tgataggtat tttattgggg aatttagctg gcatagcaat
      601 tggtatactt cttccgccct tagctgctca aactttagtt ttccatcgtg gattcacact
      661 ttataatatt ggattcacct ctgggttgat tgcgatgact tttactgctg ttttgaggtt
      721 attcagctat tcaatcgtcg aaaatacatt ggttttcaat gaatatcact ttcctctcat
      781 ttggattatt tttggctttt tttcgctgac agtcggaatt ggtttctatt ataattcctt
      841 tcggctttcg gggattcgag aaattttcga ttcatctgga aaattgacaa ctgatttcat
      901 tgctaattct ggtattggtg ctactttgat caacatgggc ttagttggtc tgatgctgtc
      961 ttcttatgtt ttgctggttg gcggtcagtt aaatggacct gtcatcggtg ctattttatc
     1021 tgcggtgggt ttcagtgctt ttggttgtca tttaaaaaac agttttccga tactagtcgg
     1081 aatatttatt gcttctttgt ttggaacttt tcatgagatc accagtactg gcatgcttgt
     1141 tgcagctgtg tttgggacag gtcttgcgcc cataagcggt ttttacggca gtttttacgg
     1201 tgtaatagct ggtgtgctac atattgctct tgtccataac gtcagcactt tgcacggagg
     1261 actaaatcta tataacagtg gtttttcaac tggtttcgtt gcaggtatcc ttgtaccgat
     1321 actagataac tttaccgctg tcagaaagga aaaaaagaca cttggaaaaa gaattatcaa
     1381 aaagaatcat cgatgagctt cttacttatt tatattccca tgaaataact gaaatacgta
     1441 ttggagtaga atttgctgag gaagggcttt tcatagagat acagggtacg gcagaaactc
     1501 agccagaaga tttagatgta cttgctgatt tgctgaacaa tcctcgggac cctagtatgg
     1561 aaatctacta tcaagaacta ttaggtaatc cccgacatga tcgggaagat tatcatctgt
     1621 taggtagttt gattgatgac gtggaaatca tgtatgccca gccgattatc aatattaaga
     1681 tctttcgacg tgcttctcat tcctattatt aaccctattt tctatttata aaagactgaa
     1741 tcaaaaaagc agctcctgaa agttcgatta aaatctactt tcaggagctg ttcaagcttg
     1801 acattattga ttttccaatc cttcatacac ttcatctgtt acaggttcta aaaactctga
     1861 attaccagca gtgatcgcca aatgttcaaa ccaactgtct tttgtcgcac catgccaatg
     1921 tttaatccct ttgtgcgtta cgacgacatc tccagccatt aatcgtcttg ctggctttcc
     1981 ttcttcctga taccagcctt ctcctccggt cacaagtaag atttgatagc cgtctgtatg
     2041 gacatgccag tgattgcgac aacctggttc aaaagttaca ttacctacac caacagaaac
     2101 ttcgggatca ttcactaaca tattcagata gctttgcccg gtaaatactt gagcgtaagc
     2161 ttcgtttttt tcacctgctg gaaaaatcac gccattgtgt atttcttcgt gttttgtcat
     2221 catttacgcc tctttcttat cttcgctatt ttcttctaaa tagatttctt ttgctaaact
     2281 aaaagctgac caagcttttg gccattctac ataaaaagct aaatgggtaa tgatttctgc
     2341 tatttcttca ctagtaatgc cattttgctt gccaatcttc agatgagctg gcaattgttc
     2401 aaaatttcct cctgaaatta atgcagatat ggtgatgata ctacgctgat gagcagataa
     2461 ttcttctctg gaccatactt gtccaaataa aacatcatca tttaattctg caaattttgg
     2521 tgcaaaatct cctaattgat ctcgtcctgc ggtttgtttt tctgccattt ctcttattct
     2581 cccttcacga ctgttaggtg attcaactat ggttctaagg caactttatt ttctattgtt
     2641 tcttcgcatt tcattttaaa tgtagtcttt cagcctgtcc aatgcttagt ttattcagtc
     2701 gatatgcttt ttaagcatag ttgctaacgc tttttttatt ttaaaaaaaa caagttatta
     2761 aataagtctt tagttggatg tttttttgag atcaatcttt tatacttatg atgaacttac
     2821 aaaattgttt gttttctgtc atatcaaaaa atggcgcagt tagttgcttt tctagtaaag
     2881 tatagttaac aaagaacctc taaggagttg atgaataatg aatattgctg cctctcgaaa
     2941 actgataaat ttcatatcca ttatcggatt aggcctatca atcgcgctga cgatttattt
     3001 tattaattta ggtgttttca aagatttgaa tgcgttaaga ggattagtag gtgattccat
     3061 cattttagga ccgattattt ttgtcttcat ccaaatcctg caagtagtta ttccaatcat
     3121 tcctggaggg atcagtactg cagctggtgt cctgatattt ggcccttatg ctggatttat
     3181 ctataactat attggaatct gcattggttc gattatcatt tttctgctag ggcgtcgtta
     3241 tggaaagcca ttcatacttt ccatgattag cgataagaca tataacaaat atattggctg
     3301 gctggataat caaaaccgtt ttgagaagct gtttgctcta gctatttttc ttccagttgc
     3361 tcctgacgat gcgttatgtt tgatggctgg attgacaaac atgtcagtga aacgatatac
     3421 actaatcatt ttgattgcta aaccactgtc gattttctta tatagtatgg ccttgattta
     3481 tggaggtcaa tatctaagtg gtcttcttgg ataatattta ttttgtaaaa ttagtcattg
     3541 attgacaatg t
//
LOCUS       DAYYME010000201         3547 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000201 DAYYME010000000
VERSION     DAYYME010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.204"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..1046
                     /gene="galE"
                     /locus_tag="ACHBFW_002758"
     CDS             57..1046
                     /gene="galE"
                     /locus_tag="ACHBFW_002758"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFM9319010.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILHVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            1058..2542
                     /gene="galT"
                     /locus_tag="ACHBFW_002759"
     CDS             1058..2542
                     /gene="galT"
                     /locus_tag="ACHBFW_002759"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFM9319011.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            2566..3546
                     /locus_tag="ACHBFW_002760"
     CDS             2566..3546
                     /locus_tag="ACHBFW_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9319012.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         1204 a          646 c          775 g          922 t
ORIGIN      
        1 ttttaaatta tatataatca acaaacacaa ttttactaat caaaggagtg ttttttatga
       61 caattttagt actaggcgga gccggttata tcggttcgca cgctgtagac cagctagttc
      121 aaaaggggta tcaagtagct gtggtagata atttgctcac aggtcacaaa caagcagtac
      181 atccagatgc tcatttttat gaaggagaca tccgggataa agaatttcta cgttccgtat
      241 ttgaaaagga accgattgaa ggagtaatcc attttgctgc aagctcttta gtaggagaat
      301 ctgtcgaaaa accattgatg tatttcaaca acaacgtata cggtatgcaa atcttacttg
      361 aagtcatgca cgagttcaat gtgaataaaa tcgtgttctc ttctactgca gcaacttacg
      421 gagaaccaaa agagtctcca atcacagaag atacaccagc taatcctaaa aatccgtatg
      481 gtgaaagtaa attgatgatg gaaaaaatga tgaagtggtg tgaccaagca tatggcatgc
      541 gctatgtcgc tttgcgctac ttcaatgtag ccggagcaaa agcagatgca tctatcggag
      601 aagatcatac accagaaacg catcttgtcc caatcatttt acatgtagct cttggtcaaa
      661 gaaaagcatt ggctgtttat ggagacgatt atgatacacc agatggaaca tgtatcagag
      721 attatgttca agtagaagat ttgattgctg cacatatttt agctttggaa tacttgaaag
      781 aaggaaatga aagcaacttc ttcaatttag gcagcagcaa aggatactca gtcaaggaaa
      841 tgctagaagc agctagagaa gtgacaggaa aagaaattcc agcagaaata gcaccaagaa
      901 gagctggaga tcctagccga ttagtcgctt caagtgaaaa agcgagagag gttctgggtt
      961 ggaaacctga atacacagat atcaaagcaa ttatcaaaac agcatgggat tggcatgtca
     1021 gccatccaaa tggttatgaa gaatagggga atgagatatg tcaatcagtc aaacgatttg
     1081 tgattttgcc acacttgcga tcgaagctgg cggatggatg gaaatggacc gcttatattt
     1141 acagaatcgt cttttaggga tggtaggaga agatgctcta gaaagcattg aagtaagacc
     1201 agttaaggaa gaatctgtag acttattgga tcagcttgtc gctaaggcaa gagaaaatgg
     1261 tgttctttca gaattgccag cagaacaaga gatttttgaa gcgcaattaa tggattttct
     1321 cacaccgcca ccttcagtcg tcaatgcatt ctttgcacag cactattcga aaagcccaga
     1381 ggaagcaact gagtatttct ttaatttaag taagaaaaat gactacatca aaacaagagc
     1441 catcgcaaaa aacatcaaat ttccggcaga gtcggagtat ggtgaactgg aaatcacaat
     1501 caatttatct aagccagaaa aagatccaaa acaaatcgca gcagaacgcg aggcggtaaa
     1561 agtagactat cctacctgca tgctttgcat ggaaaacgaa ggatatagag gtcgcttgaa
     1621 ctatccagcc cgcaccaatc accggattat tcgaatgaat ctagacggtg aaagctgggg
     1681 attccaatat tctccttatg cttattacaa tgaacattcg atcattttat cagaacaaca
     1741 ccgcccgatg aaaatcacaa aggaaacatt tgctagactg cttcggatta ctgaagtctt
     1801 gcctcattac tttgttggtt cgaatgctga cttgccgatt gttggaggat cgattctttc
     1861 tcatgatcat tatcaggcag gacgtcatga atttcctatg gcaaaagcac cgatagaaaa
     1921 acttatcaaa ctagcagatt atccgcaagt catcgcagga gtcgtgaaat ggccgatgtc
     1981 tgtgatccgt ttgcaatcaa cagagaaaaa attgttgatt gaagcagcta actcagtatt
     2041 ggaaaagtgg aaagcatatt cggatgaaac agtatctatc agagcttttt cagcagatgg
     2101 tacaccacac catacgatta cgccaattgc acgaagaagg gacaattact ttgagttgga
     2161 ccttgtcctt cgagacaaca atgtttcaga agaacatcct gatgggattt ttcatccaca
     2221 taaagatgtc caacatatca aaaaggagaa cattgggttg atcgaagtaa tggggcttgc
     2281 tgttttacca cctcgtttga aaccagaact agtagaagta gagcatttct tattaggaga
     2341 agaaaaccag atggctgaat atcatcgtcc gtgggcagaa caattaaaaa aagaacatcc
     2401 tgatttgacg aaagatacgg tcaccgatgt cgtacaaaaa tctgtaggtc aggtttttgc
     2461 acgtgtactg gaagacgctg gagtattcaa gcgagatgaa aaaggacagg cagcgtttga
     2521 acgatttact gcaattttat aaacgtaaaa aaggaggaga gacaaatggc aacaattaaa
     2581 gacattgcgc agttggcagg agtttctcct gctactgttt caagagttct caactatgat
     2641 cctgatcttt ccgttggctt agccacaaag caaaaaattt ttgaagcagc agaacgctta
     2701 aattatacga aacatcgtaa aaatgcagca aatactaagc aaacgcttcg cctgatccag
     2761 tggtacgacg atacggaaga gcttgaagat ctttattatt tatctattcg tctaggaatc
     2821 gaaaaaaagg cagaagaagc aaacgttact ttattgaaag aaacgtggga ttccattagt
     2881 gatcttccga cagatggaac gatcgcacta ggaaaattcg acgaagctca gttattagaa
     2941 atgaaaggat cccaaaatgc cttgcttttg gtagattctg acggcacttc tcaaggaatc
     3001 gactctctag ttgtcgactt taagtcaagt gttcaaaaag tcattgaaca ttttgtctcc
     3061 cataaagcgc aacatatcgc tatgcttgct ggaacagaat atacgaagaa aaaccatcag
     3121 cggctaaaag atcccagaat tatggcattt caacaagttt tggcagaaaa aggctacgac
     3181 gaaggtccaa ttatcgaagc agatttttct gtcgagtcag gatatcaagc agtgaagcag
     3241 tatttaaaaa cgaatccgca tgttcctgat gcgctttttg cagctaatga tgcattagcg
     3301 attggcgcac tgaaagcgat tcaagaaaaa ggtttagtgg taccagaaga tatttctgtg
     3361 atgggtttca atgatgtcag tgtagcgaaa tatgtaacgc cctctctttc tactgtaaaa
     3421 gtttataccg aatggatggg agagctagca gtggagacga ttcttcaatt aagtaattcc
     3481 aaagcaccgg tttcaagaaa gattattgtg gaaacagagt tgattattcg agaatcaacg
     3541 aaataaa
//
LOCUS       DAYYME010000202         3364 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000202 DAYYME010000000
VERSION     DAYYME010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.205"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..693
                     /locus_tag="ACHBFW_002761"
     CDS             <1..693
                     /locus_tag="ACHBFW_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9319013.1"
                     /translation="NTKVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVK
                     YYDEIANSLTEALYSTIRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFL
                     RVGVQAKAVLDPHFQAQVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAIT
                     NYIHSPIGKSADLVLQTAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELRE
                     KVLRSIIDKSIE"
     gene            923..1153
                     /locus_tag="ACHBFW_002762"
     CDS             923..1153
                     /locus_tag="ACHBFW_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFM9319014.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(1376..1474)
                     /locus_tag="ACHBFW_002763"
                     /pseudo
     CDS             complement(1376..1474)
                     /locus_tag="ACHBFW_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1739..2194)
                     /gene="tnpA"
                     /locus_tag="ACHBFW_002764"
     CDS             complement(1739..2194)
                     /gene="tnpA"
                     /locus_tag="ACHBFW_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFM9319015.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2437..3364
                     /locus_tag="ACHBFW_002765"
                     /pseudo
     CDS             2437..3364
                     /locus_tag="ACHBFW_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
BASE COUNT         1106 a          596 c          588 g         1074 t
ORIGIN      
        1 aacacaaaag ttggcgatgc tactattatt cgcttttgtc aaaaaattgg tttttctggt
       61 ttttctgatt taaaaattga aattgcaaag gaagacttta gtcaaaaaaa agaacatccg
      121 actagtgtaa agtactatga tgaaattgct aatagtttaa cagaagctct ctattccact
      181 attaggcttt tagacgaaga aaaattgaat gaagccatta aaatgatcac tcaatcaaaa
      241 aacatatata tatttggagt gggatctagt ggaaatacta gtcttgattt agaaaatatg
      301 tttcttcgcg taggagttca agcaaaagct gtactagacc ctcattttca agcccaagtc
      361 gcttctttat taacagttaa tgatttagta atcatttttt ctttatctgg gaaaacgaaa
      421 gatacttatg attcattgaa aatagctaaa aaaaatggcg caaaaattat tgctataact
      481 aactatattc attcacctat aggaaagtct gcagacttag ttttacaaac agccattgaa
      541 gaatttctta atggtggatc tttagctgga aaaatatcac aactttatat ttgtgatcta
      601 ttagttcatg gttatgaaca aaacaataat attgattcag ttgaattaag agaaaaagtt
      661 ttgcgctcta ttattgataa aagtattgag tgaactaccc ccacttagct aaacctaacg
      721 gtttttaacg attgaagtag gcaattcttg ggatgagagc atacttgata gcttattttt
      781 tactaatagc ggtggctctt attgaccaaa tatccctgta gttcctacgg ttcttgattt
      841 tatctaaaga tatttttatc gtggaagcta ttgatcaact tgaaagaaaa acgtgtagct
      901 cattatcact agtttatcga gtatggatga agcaatggat catcctctct taaataaatt
      961 tattaaggat ctgatccttc aaattttttc catgatcgat gagcaggaac ctactgaatc
     1021 taaacgacat cgggcacaag gaattattaa gattgctaag cgaatgtttt ctataaaggg
     1081 tgttctaagc tatacagtgt taattcaaaa gattcccaat catcttgttt ataagaacaa
     1141 cgttgaagat taaaaaagtg tttcaatgct aaaattgcaa aatacttcaa tgttatgaga
     1201 caaactgtct ataagaatca atttccaccc atttcatcac ttgttaaaag tttatgatcc
     1261 ttttgatttt tatattaatt tatctgtatt taatacagat tttatttatt agttcatctg
     1321 cctattgtat tttattttgt tgccttacca tctgaagtaa atatactctt aattgtcaaa
     1381 ttaagttaga cagatcatca atacttacgt aactcaaaaa tacttccaaa ggtgttcgat
     1441 aatccagtga ttttctagga agattatttc tgcgccagca ttttctaatg tttgaatttg
     1501 tgttttgtaa tcttggttgc ttgtgctaat tcttgaatag ccgtaaattg ccaaaatttc
     1561 atcacccttt ctggacataa gtttctgaca ccagttaaaa tcaagtctat cagtaaccca
     1621 tacacccgtg aggacgggtg tatttttaat ccccctagaa ggggtcgaca ctcgctgccc
     1681 cttaaaaggg gatttttaaa agaccgccaa ctgctcatct tcactcactt acttctcttt
     1741 attttttaaa tggttctacg tactctcttt tacttataat atatcgaatg cgatcatcag
     1801 cttcttgttc acggatgtat ttttcatttg tcttttgatt caatccgact gtactcacat
     1861 agtatctctt cgaccaaaag gatttgtttc cgtatttata tttgagattt gcgtgctgct
     1921 cgtggattag taccgcacta cgacctttca gataccccat aaaactggat atacatattt
     1981 ttggtggtac tcgaacaagc atgtgaatat gatctagcat tgcatgtgct tctatgattt
     2041 ccacatcttt catttcgcac aacttcctaa gtatttttcc aagatctttt cgtaattgcc
     2101 catagattac ttttcgttga tattttggtg taaaaataat atgatactta caattccaac
     2161 gagtatatgc taaactttta tcgtcatttg acatgaaaaa actcctttcg atctgatgag
     2221 cggttggtag ccactcacta gtatatcgaa aggagttttt atcgctaaag atacttcttc
     2281 cacgcgtaga aacgcgcggt tttcttgttt cgcacgcttt acgcacaaaa ctgactgaag
     2341 tcattaagaa aataaaaggg tctaaaactt ttaagttttg taccgacccc caaaagttag
     2401 accaaaaaat ctaacttttg gaggtcggta ttttttatgg ctaagtatac ttttgaactt
     2461 aaattaaaga tcgttcatga ttacttggat ggtaaaggcg gaagtgatta tctggcaaag
     2521 aaatattcca ttaaggctcc atcacaagtg aagcgctgga tcaatgccta tcaagaattt
     2581 ggtgaagagg gtctagtaag aaagcgacaa aagaaaaaat attctgttca atttaagctg
     2641 gatgcgatag agttatatct aacaagtgag ctgtcctatc gtgaagttgc caacacactt
     2701 aacatgaaca accctaattt gatcgctagt tggatgcgtc aattccgtga gggtgggatc
     2761 gatggactct caaaaacgaa aggatgtcca cctatcttgt ctaagaaaaa tgaacccaaa
     2821 aataagaaaa aatctacaac taaagcaacg tctaaagaac gtgaacgcat cgaggagttg
     2881 gagaaacgag tccgttccct tcaaattgaa aatgcctttt taaaagaatt gaggaagctg
     2941 cgaaaacagg aagcccaaca acgacgaatg aagcaatcgc acgaatcatc gcaagcctcc
     3001 gaggaccgtt taaattagtt gagcttctag aaactttaaa gttccctaag gcaatcttta
     3061 tgtactggca aaaacgattg gaccgtaaaa atccagatca agagattgaa gcggaaatgc
     3121 ttaaagttcg ggagaagcac aaggattatg gctgtttacg gatgaccaat gaactaagaa
     3181 accgcggttt ctctatcaat aaaaagaaag ttcagcgcct gatcaagaag ctaggaataa
     3241 aagtaacgac atacacacgt aaatcacggc gctacaattc ttatcgagga caagtcggag
     3301 cagtagccaa aaatcggatc catcgtcgtt tctacaccag tatctgccat cagaaaatca
     3361 cgac
//
LOCUS       DAYYME010000203         3316 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000203 DAYYME010000000
VERSION     DAYYME010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.206"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..431
                     /locus_tag="ACHBFW_002766"
     CDS             <1..431
                     /locus_tag="ACHBFW_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFM9319016.1"
                     /translation="PNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTILKECKFMI
                     GNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKIQDPQFIKS
                     LKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            404..1081
                     /locus_tag="ACHBFW_002767"
     CDS             404..1081
                     /locus_tag="ACHBFW_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM9319017.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1032..2213
                     /locus_tag="ACHBFW_002768"
     CDS             1032..2213
                     /locus_tag="ACHBFW_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM9319018.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            2226..3203
                     /locus_tag="ACHBFW_002769"
     CDS             2226..3203
                     /locus_tag="ACHBFW_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9319019.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            3203..>3316
                     /locus_tag="ACHBFW_002770"
     CDS             3203..>3316
                     /locus_tag="ACHBFW_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM9319020.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSS"
BASE COUNT         1240 a          413 c          636 g         1027 t
ORIGIN      
        1 tacctaatag tgatgctggt agcgaaatta tcagaaaaat gtaccagaaa tatttaaaaa
       61 cggatattat ttatattacg aatttagagc cggaggaata cctgactata ttaaaagagt
      121 gcaaatttat gatcggaaat tcttcttctg ggataattga agctgcatca ttcaaaatac
      181 cagtaataaa tttaggaacc agacaaaatg gacgagaaca atctaaaaat attataaatg
      241 caaatctaaa tgaaaatgaa attgaaactg ctttgaataa aatacaagat cctcagttta
      301 taaaaagctt gaaaaatgtt aagaacatct acggtgacgg caacagttct agaagaataa
      361 caaaaacatt gaatcaaata ttgaatgatg gggaagatat taagtggatt caaaaacaaa
      421 tagcttatta aaatatacta gtgatccata cattattatt ggggctggcg gacacgcaaa
      481 agtcattatt gaaaccttaa gattagaaaa taagttaata tatggatttt ttgatgataa
      541 gcaaaataaa atgcaagatc tgaaatatct tgggaaaata aacgaattga atagctgggc
      601 ggatagcaaa aaaaaaatct ataaatttat tgtagctatt ggtgactgtg tagtaagaaa
      661 aaagattata aaaaaattga atatgccaag tgagcaatat ggaatagctg ttcatccaag
      721 tgctatagtg gcaaaagaaa tttcaattgg ttatggaact gttatttttg cgaatgctgt
      781 aattaaccca gatgctgtaa ttggagagca tgctataata aacacaggta gcattgttga
      841 gcatgattgt agaattaata actatgttca tttatctcca ggtgtatgct tagcgggtgg
      901 agtacatgta ggtgtaggaa ctcaaatagg aattggttct caatgtatcc agatgaagaa
      961 aattggatct tggtgtatga taggcgcagg gagcactatt gttaaggata ttccctcaca
     1021 ttccttagct tatggcaatc cagctaaaat taagaaagaa ggaattaatt ttgaattcta
     1081 gaattttatt atcaagtcct catatgggag gaacagaaga aaaatatgtt gctaaggctt
     1141 ttaaagataa ttggatagcg ccattaggtg aaaatgtgga taaatttgaa gaaagtatta
     1201 caaaatatac tggttctaag ggcggagctt ctgcgttaag ttcaggtaca gctgccatac
     1261 atcttgcctt aagactgctt aatgttggaa aaaacgatat tgtctttgct ccttcattta
     1321 cttttatagc tacagtaaat cctattttat atcaaggggc tgtaccagtg ttcattgact
     1381 cagaacctaa tacatggaat atgagccctg ttgcactaga gagcgcatta gaagaagctg
     1441 aaagaaacaa ccaattacct aaggcaatca tcgtagtgaa tttatatggt caaatggctg
     1501 attatgaacg aattttagct attgcaaacc aatttaatgt tccagtgata gaagatgcag
     1561 cggaatcgtt aggttctact tataagggaa tttcgtcagg cttatttggg acaattggga
     1621 tctattcatt taacggtaat aagatcatca caacctcagg tggaggagct ctagtgtcta
     1681 gtgataaaaa atatgttgag cattcaagat ttttagcaac acaagcaaga gatcaagcac
     1741 cgtattatca acatagcgaa ataggttata attaccgtct aagcaatatt tctgccggta
     1801 tcggacgtgg acaaatggaa gtattgaatg atagagtagc taaacgaaga gaaattttca
     1861 atcgatataa ggaagctttt tctggaattg atagaattga aatgatgcca gaagtggatg
     1921 gcatggtgcc aaatcgttgg ttgtctactt taacgatcaa tgattataat tctattgatc
     1981 aagtagttaa cgtaataaat aaactaaatg aaaaaaacat tgaaacaaga gctttgtgga
     2041 aaccactaca tttgcaacca gtatttcaag gggtaaaaat gtattaccat gataataatc
     2101 ttagtgtgtc agaaaagtta tttaatagcg gcttatgctt accttctgga agtaatatga
     2161 cagatagtga acaagaattt gttattgatg agtttaaaaa aatattcaag taggtgtgat
     2221 attttatgaa cataggaatt attggtttag gttccatggg gaaaagaaga cttagactca
     2281 ttcaagaaca ctttaatgat attttactat gtggtgttga tactaatgag gcgagacgaa
     2341 aggaagttga aaaggaattt ggaataccaa cttataatag tctaacagag gcaaaaaatt
     2401 tgtttgaaat aaattcagtt tttgtttgta catcccctat aacccatgaa tttattataa
     2461 aagaggcttt aaatttagat tgtaatatct ttacggaaat caatttatta aatgaatatt
     2521 atgatgaaat aatcgaagta gctaaaaaga aagatagatt actttatcta tcttctacat
     2581 ttttgaagag aaaagaaatt caatatatta agtctaaagt gacgcataat aaaaaaattt
     2641 cttatcaata ccatgtagga cagtatttac ccgactggca tccttgggaa gattatcgca
     2701 atttttttgt ttcaaacaaa aaaactaatg ggtgtagaga aatattcgct attgaattac
     2761 cttggattat agatacattt gggaaagtta aatcgtttac tgtaatgacc cagaaattat
     2821 ccaatcttga tattgattat tcggacagtt tcactcttat tattgagcat gaaactggta
     2881 tagttggaac tttaagtgta gatgttgttt caagagtagc taaaagaaat ttaactataa
     2941 ttggtgaaaa gattttaata gaatgggaag gtaacccgga ttctctattt tcatggaatg
     3001 aagaaacttc tagtatgaca gatattcaat tgtacgatga ggttatgcat gataataatt
     3061 attcacatac tattattgaa gatgcatatt tagaggagat taaagaattt ttcacgcttt
     3121 tggaaaagaa aatggaaccc caatatactt ttgaaaaaga taagtatatt attaacttaa
     3181 ttaacgacat tgaggtaaat taatgatgaa agtaattttt tttgggttag ggtcaattgg
     3241 aaagagacat ctaaaaaatc taataagcta tggaaaaaag aattctatta attttgatat
     3301 aacagcttat agtagc
//
LOCUS       DAYYME010000204         3204 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000204 DAYYME010000000
VERSION     DAYYME010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.207"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            142..831
                     /locus_tag="ACHBFW_002771"
     CDS             142..831
                     /locus_tag="ACHBFW_002771"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM9319021.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            845..2347
                     /locus_tag="ACHBFW_002772"
     CDS             845..2347
                     /locus_tag="ACHBFW_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9319022.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2360..2836
                     /locus_tag="ACHBFW_002773"
     CDS             2360..2836
                     /locus_tag="ACHBFW_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM9319023.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(2933..3043)
                     /locus_tag="ACHBFW_002774"
                     /pseudo
     CDS             complement(2933..3043)
                     /locus_tag="ACHBFW_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1058 a          433 c          618 g         1095 t
ORIGIN      
        1 caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact ccatttttat
       61 ctttactttt atgaaagcgg atgctatatt gtttttggag ttgttatcca aaaataataa
      121 aaaattggag tgtttaggat aatggataaa aagaggtttt tagatcttat ttcaaaaggt
      181 ttgattgttt cctgtcaagc attacctggt gaaccatttt acaaagagga agaaagttta
      241 atgccttatt ttgctttggc tgctcaaagg gctggagcag ttggtatacg tgcaaattct
      301 gttcgagata ttattgaaat tagaaagtgt gtatcattac caatgattgg aattattaaa
      361 agagattatc caccagaatc tccattcatt actgccacta tgagagaagt agatgaatta
      421 gtagaaaccg gtgttgaggt aatcgctctt gattgcactc ttagaaatcg acatgatgga
      481 ctatctataa attcttttat tactaaaata aaagaaaaat atcctcatca gttatttatg
      541 gcagatattt ctacatttga agaaggcaaa aatgcatata aagcaggaat agattttgta
      601 ggaactactt taagtggata tactgaggaa agtaaaaaac aagatgggcc agatatagat
      661 ttgattaaaa aattggtaga agaagggatt tgtgtaattg ctgagggaag gattcatact
      721 ccagaacaag caaaagagat aaaaaaaata ggagttgcag gtattgttgt gggtggtgct
      781 attactcgac cacaggaaat tgcatcaaga tttgttaaag cattaagtta gggggagaaa
      841 taaaatgttt aaaaaatttt cacaattagg acgcgctttc atgttaccaa ttgctatatt
      901 accagtagcc ggattactat tgggactggg aggagcgtta acaaatgaga gtgctataaa
      961 tgcttatcca tttttagatc aaccgtggtt gcatacaatt ttaagtatta tgagttatgc
     1021 tggaaatgca gtttttgcaa acctagcact aatttttgca attggtattg ctgtaggact
     1081 tgcaaatgga gataaaggaa cagctggttt agctggtgga gtagcttatc tagtatatac
     1141 tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa gatgcaacac tagatacagg
     1201 tgtagtggga tcaattgttg ttggtggtac tgttgcattt ttacacaatc gttatagaaa
     1261 aattgaatta ccacaattct taggtttttt tggaggatca agatttattc cgattgtttc
     1321 tagtttagct gcaatcatta ttggttcttt cttttattta atttggccac caattcaaaa
     1381 tggtttagta gttgctggtg aacaaattgc ccaaatgggt agtttaggaa cttttttata
     1441 tggtttttta ttgcgtttaa caggtgctgt tggactacac cacaccattt accctttgtt
     1501 ctggtataca tctttaggtg gtaccgaaac tgtagctgga tcaacaattg ctggagccca
     1561 aaatattttc tttgcccaac tagctgatcc aaaccacact ggtctgttta cttatggtac
     1621 tagatttttt gcaggaagat ttgcaacaat gatgtttgga ttaccagcag catgttatgc
     1681 aatgtatcgt gcaattccta agaaaaatcg taaaaagaat ggtgggcttt attttagcgg
     1741 ggcccttaca tcatttttaa ctggaattac tgaaccagtt gaatatatgt ttttatttgt
     1801 tgctccttgg ctttatgtga ttcatgcatt cttagatgga ttatcttttt attttgctga
     1861 tatcttaaat attcgtgtag ggaattcctt ttcaggtggt ttgatcgact ttttattatt
     1921 cggtgtactt caagggaatg acaaaactaa ttggttaaaa ttaattcctt ttggtattgc
     1981 atgggcattt atatatttct ttgtttttac attctttatc aaaaaattta aggtagctat
     2041 tcctggaatg gaagaagaat cagaagttca ttctgaaaaa gtaataaaca aaaatacatc
     2101 attacatgaa gagtctctta tcattattga agctcttggt ggaaaagaaa atattgagtc
     2161 tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg aatggggaga ttattcaaaa
     2221 acaaacaata aaagacactg gtgcaactgc tgtcttcgaa gtgaagggtg gcatccaagc
     2281 tgtttttgga gggaaagctg acttattaag tcaagaaatc aatcaaatat taggaaaaga
     2341 taattaggag gtttttaata tgtttgaaat tttcaaaaag aaaaaacaag aaacttttta
     2401 tgcaccttgt tccggaaaaa tcatttccat cacagaagta aatgatcctg tcttttcaca
     2461 aaaattacta ggtgatggtt ttgccgtcat acctgatggt tcaaaaattt atgctccatt
     2521 ggctggtcga gtaacaaata tatttcctac taaacatgct attaatataa aaagcaactc
     2581 aggcattgat tatttgattc atattggaat tgatacagtt gaattacaag gagagccttt
     2641 ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa acctcgttgg ttgatgtgga
     2701 ttttgacaaa ataataaaat ctggtaaaga tacttctgtt atagttgttt ttccggagaa
     2761 aaatcagttc aatgatttaa taatagataa tggtcgaatt gctcatggga attcatgtgg
     2821 aaaaatcact ttttaaaaaa gtgtatataa aaagctataa aataagctaa ataggatatt
     2881 tgtcaaatga agttggtgaa cagaaagaac tgttttcttt ctgttcattt ttttatttta
     2941 ttagtaatga ttccccatgc caaatcatga tccgtaattt aaagttataa aagcttcgaa
     3001 atccattggc atttcgttgg aataccttga tatgattatt caaatataaa atatagagag
     3061 aagagagaga gggaagatat ttataaaggg caaagttatg tttactcaac ttaggaaata
     3121 gaaatagatc aatttatatt ctcttgtcaa tcgaatatga accttagcac tatataccaa
     3181 atatagtaaa atgctgaaag atgt
//
LOCUS       DAYYME010000205         3200 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000205 DAYYME010000000
VERSION     DAYYME010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.208"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..204)
                     /locus_tag="ACHBFW_002775"
     CDS             complement(<1..204)
                     /locus_tag="ACHBFW_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFM9319024.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILI"
     gene            complement(176..1375)
                     /locus_tag="ACHBFW_002776"
     CDS             complement(176..1375)
                     /locus_tag="ACHBFW_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM9319025.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1505..2881)
                     /locus_tag="ACHBFW_002777"
     CDS             complement(1505..2881)
                     /locus_tag="ACHBFW_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9319026.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2881..>3200)
                     /locus_tag="ACHBFW_002778"
     CDS             complement(2881..>3200)
                     /locus_tag="ACHBFW_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9319027.1"
                     /translation="FELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPT
                     GNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNL
                     EVE"
BASE COUNT          980 a          614 c          460 g         1146 t
ORIGIN      
        1 gatcagaatc actggatcga tacttgggcg acctaaatcg gagtaagtgg cttcgacgat
       61 tggataaata aattcaaaat caagcgcggc atcaatcttt cgaacgagat gatcttgagg
      121 aactagatca tcaatcgtaa gcatcgcaaa ttgatttcta ccattcgtat tgtttttaga
      181 catcatagct atcactcctt gcattcgttt tcataaatct attataaaag aaaagactgt
      241 agacatcatc cataaaatgg actttgtcta cagtctgcgc attctcatta gagaacactc
      301 ccttttttgt gacttttctg aagctagagt gaatactttc aactgcatta gtagtgtaca
      361 tgatttttct aatggcgctg ccataatcaa agagttgttc aacatgagaa aagtttcgtt
      421 tgcatacatc tactgcacca gaataatgag accaccgatt ttgaaaactg tcgaaggcag
      481 cgtgtgcagc gtttaaagaa gaagcactgt agaatttttt catgtctcga cagacttcct
      541 tataatcctt acttgggagg tatcgtaacg catttcgaac aagatgaaca atacagcgct
      601 gcacaatgac agaaggaaaa attgcttttg cgccttcttt gagaccggaa acaccgtcca
      661 tcgaaatgaa aaaaacatct tctacaccac gtactttcag ttcatcaaat acttgcatcc
      721 agcgattttt agattctgtt tgattgagcc ataggcctaa aatctccttg tttcctttaa
      781 gatcatagcc aagaatggtg tacacagcat attctttagc ttcatagttt tcgcgtaaag
      841 tgacgtacat acaatcaacg aatagaaatg cataacactt ggctaatggc cgagcttgcc
      901 attcttccaa ttcaggaagg acggcatccg tgatatcaga aatcatttcg tgggaaatat
      961 caaagccgta aatatcttca acggttgcgg cgatatcgca ttgactcatt cctcgtgcat
     1021 acatggaaag aaccttacct tcgatgtcgg agacatctcg ttttctatta ggaatcaact
     1081 caggttcaaa agaagcctcc cggtctctgg gaacatcaat gtttacttta ccaaagctcg
     1141 ttttaagcgt cttattgcca taaccgtttc gtcgattgtc atgtttcttt ggctctttag
     1201 aatgggcgtc ataccctaaa tggttattca attctccttg aagcattttt tcaaataagg
     1261 gaccaaacac atcttttaaa acatcttgca tatcctctac agattcgggt tgataagtat
     1321 tcaaaatgga ttcagctaac ttctcagcat caggatttct tttctttcta gccatcgtgt
     1381 aatcacctct tgatcttatt ttagaaaaat aaaaaccgtg aagcaaccac ccgcctagga
     1441 ttgatggtta cttacacggt ttatattaca ctctcatttt tggtgctaaa aagcaccgaa
     1501 aattttaatt attgtcaatt aataattctt ttggattcct tcttaatata ttgaatgaag
     1561 aaataatcaa tgatacaaga agtactaaac tcataaatat aactacataa atcatgaatt
     1621 taggtaatac attgatatct aaacttgata atgtcttgtt aaatccttca gcttctgccc
     1681 caccacctaa ttggctagat gcagattgtt tagctatttg tttagctata tcaccagtaa
     1741 ccttattcaa tatattattc ccaagcttgt cagctgtata ttgagctaag aaataggaac
     1801 caacgaatgc ggggattgat ataaatacca tctcaattag gaattgacca aaaatttcta
     1861 attttgatat acctaatgat agtaatactg ctatttcttt ctttctagca ttcatccata
     1921 agaataataa taatgaaact acaactcctg caaatattaa tgagccaaaa aataatttat
     1981 ttgaaattga gtaaatacct gatatagatt gttgtaatgc agggtaattt gatgaacttt
     2041 taatcaaatt atattctctc caatttatat ctaatttccc aagatctttt attacactat
     2101 caagattctt atcacctttt acaaagaatg ttgcatcttg gtatacagct gtatcttcgg
     2161 tattaccata aactttagca gctgtatgaa tatcagtgat taatgtattt tcgtatagtt
     2221 cttgtgctgc acttactcca ccgctattat gcccatcgaa tagaccttta atttctactt
     2281 ctactgtttc atctgcacct ttctcattat cagcatcaaa taaattagat tttattttta
     2341 tcttatctcc aactttaagg ttattttttt cagctaaatc tttatgcatt aagattttat
     2401 ttttatcttc attttctaaa tgctttcctt ctactaattt atatgcttct gatacaaatt
     2461 ttgtttcttt tgttgagtcg ttaaccccag ttaacataac tgttctttta aaattctttg
     2521 ctctttcagg tgattgattc gcaagagtct cttgagtttc aataatatca taatcaacta
     2581 aatctgcaac actgtttatt ctttttacat aggagtctat actatctgtt tgagatatct
     2641 ttttaatatc ttcacctttt acatttcccc cacctctagg tgttcctgga tttacttgtc
     2701 tatttatctc catagaaaaa ctatttgtta tattagcaaa tgtttctttt gaagctctgt
     2761 ccgtagcatc tttaatagat aaactaatga gacttaaagt tgacatagat aaaataacta
     2821 atatgataat taatgatttt aaactttttc tagttacata agcaaatgca ttttttatca
     2881 ttattcaacc tccaaattca ttttatttac ttttttcaac tttttaccac ttagttctaa
     2941 aatgatatcc gcagaatctg ctacttcctt actatgtgtt acaactatta cacatttatt
     3001 tctatcttta gctaatgtct ttaatatatt aattatttct ccagcagtaa cactgtctag
     3061 attaccagta ggctcatcag ctagtattat tggagcatct gataccaatg ctctagcaat
     3121 agctactctt tgttgctgtc caccagataa tttcataaca tttcttttta tttgtttttt
     3181 atctaaacct agttcaaaca
//
LOCUS       DAYYME010000206         3165 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000206 DAYYME010000000
VERSION     DAYYME010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.210"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(66..2558)
                     /locus_tag="ACHBFW_002779"
     CDS             complement(66..2558)
                     /locus_tag="ACHBFW_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFM9319028.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2565..3032)
                     /locus_tag="ACHBFW_002780"
     CDS             complement(2565..3032)
                     /locus_tag="ACHBFW_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFM9319029.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT          840 a          697 c          518 g         1110 t
ORIGIN      
        1 aatcttgtca caacctctta atcaagtctt actattataa aaattcagct acacaggcca
       61 agtaactata gggaacctac tgtttccttt ttaggtccgc gaacattcaa tgtgatctta
      121 ccttttgaag cacctactgt tacgtggtca cctaaacgaa tttctccgcc aagcaaagcc
      181 tcgctcaatc gatcttcaat ttctttttga agtgcgcgac ggattggacg tgcaccatat
      241 tcaggatcaa atccagcttt cccgatcaca tcgattgcag atggtgtgat tttcacttga
      301 atatcttgtt ctttcaatcg tttgataatc gctttgctca tgatttggac gattgtatgg
      361 atttcatctt tactcaatga atggaagacg acagtctcat cgattcgatt caagaattca
      421 ggtcggaaag ctttctttaa ctcttctaag atacgttttt gcatggcttt gtgatcttta
      481 gtaacatctt ggacattgaa tccgacattt ttttcttctc gaatttgtgt cgctccgata
      541 tttgaagtca taatcatgat tgtatttcta aagtccactt ttctaccttt agaatctgtt
      601 aaatgaccat catccaatac ttgcagaagt aaattgaaaa cgtctggatg tgctttctcc
      661 acttcatcta agagaatcac tgaatatggt ttttgacgaa ttttttctgt caactgtcca
      721 ccttcatcat accctacgta tcctggagga gacccgatta atcggcttgt actgtatttt
      781 tccataaatt ctgacatatc aacacggatc agggcatctt cacttccaaa catgacttct
      841 gaaagtgctt tggccaattc tgtttttccg acaccagttg gtccaaggaa catgaatgaa
      901 ccaattggac gatcaggatc tttcaatccg cttcgtgcac ggcggatcgc acgtgaaact
      961 gctttgacag cctcatcttg accaacgaca cgttcatgca gaagtccttc cagttctaac
     1021 aagcgttcgc tttctttttt ctccaactgc tgtagaggga caccagtcca ctccgataca
     1081 actgtagcaa catcttcttc tgttacacga tcagaataac cttgctcctg ttttgcttca
     1141 attgcagaaa ctttttgcag ctttttacgt agtgcttttt cttgtctgcg taattgagca
     1201 gcattttcaa agtcttgttt ttggatcgct gcttcctttt cttgtactag ttcatcgatt
     1261 tctagtttga tcacagttga ttttgttaag tgatcggttt gatccaaacg gactttcgct
     1321 gctgattcat cgatcaaatc aattgcttta tcgggtagtt gacgagaagt gatataacga
     1381 acagaaagat tgactgctgc tcgtacagct tcatctgtga tctccactcc atggtgctct
     1441 tcatatcttg tacgtaatcc ttgaagaatc acttctgctt cttcaggagt tggctcatct
     1501 acttgaatgc gagcaaaacg tcgctctaat gcagaatctt tttcgatata tttttgatat
     1561 tcatctaggg tcgttgctcc gatcgtttgt aattctcctc gagctaacgc cggtttcaag
     1621 atattcgaag catcaattgc tccttctgct ccacctgcac caattaaggt atgcaattca
     1681 tcaatgaata agattacttg accatcatta tagatttcgt cgatgacctt tttcattcga
     1741 tcttcaaatt caccgcgata tttcgtacca gcaaccaaag atcccatatc aagcatcatc
     1801 aggcgctttt cttgcatgtc ttctggcact tcacctaaaa tgattttttg agctaaccct
     1861 tcagcaatgg ctgttttgcc tacaccaggt tcacccacca gtacagggtt attctttgtc
     1921 cgtcggctaa gaatctggat aagccgtttc acttctctcg aacgaccaac aactgggtcc
     1981 agcttgtttt cttttgctaa tttcgtcaaa tcacgtgcta atgaatctag cgtaggtgtt
     2041 ccttgcggtg tttgccgttt atcttgtaca tttacatttc tacgacgatt cattcctttt
     2101 ccagcagaat caccagctag ccccattttt tgtttcagaa cttgacgggc ttttgaaagg
     2161 ctgataccta agttgatcat gattcgagta gccatgatac tttcatcttt taataagctc
     2221 atcaaaatat gttctgttcc aactttttga gaaccagttt tcttcacttc gctgctagct
     2281 aaagcaaaaa cttgtttcat tcttggtgaa tatggaagaa agactccttt agggtaagaa
     2341 cggataccac cataaccaat cagatgttca atttcttcat aaatttccgt ttcattcaaa
     2401 tccatttcac gtaatacttt tccagcaatt ccattttgct cgaccactaa ggctagtaaa
     2461 acatgttctg aactaactga ttgatgtttc aaccgttttg cttcttcttg tgcaatttct
     2521 aatactgctt gggctctttc agtaaaaagt tcttccatct aatctcactt cccatcttca
     2581 taacttaaac gttccagaaa tgctcggaga atccgagcac gtgtatgatc ttcatattca
     2641 ttgaaattca atgtgttttt tgcaattgca ctcaacatca agtttccttc tttttttgta
     2701 attatttggt cttcataaag tttttgaata atagcaaaag catttttctc actgattgta
     2761 tcatcaaata actgatttat ctgctcaagt aattgttttt tatctgaaat ccttactttt
     2821 gcaattcgta tataaccacc accaccacgt ttgctttcta ctagataacc acgttggatg
     2881 gtaaaacggg tattaatcac atagttgatt tgtgaaggaa cacagttaaa gaggtctgcc
     2941 atttcaatcc ttcgaatctc gatcatttca ctattttcca gaattttctt tatatatgat
     3001 tcaattaaat ccgaggtatt ctgatgtgcc atcttattca ttccctttga ctaatattga
     3061 ccttaattat aacgaatttg ataatagaaa gaaagaagaa cgctttttgt taaataaatc
     3121 ctttagaatc aacaaaaaaa aatacggaat atactattcc gtatc
//
LOCUS       DAYYME010000207         3154 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000207 DAYYME010000000
VERSION     DAYYME010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.211"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(206..574)
                     /locus_tag="ACHBFW_002781"
     CDS             complement(206..574)
                     /locus_tag="ACHBFW_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFM9319030.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            724..1206
                     /locus_tag="ACHBFW_002782"
     CDS             724..1206
                     /locus_tag="ACHBFW_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFM9319031.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1243..2253
                     /locus_tag="ACHBFW_002783"
     CDS             1243..2253
                     /locus_tag="ACHBFW_002783"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFM9319032.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2368..3081)
                     /locus_tag="ACHBFW_002784"
     CDS             complement(2368..3081)
                     /locus_tag="ACHBFW_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319033.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         1068 a          510 c          615 g          961 t
ORIGIN      
        1 tgaaaaaata attttactaa gtggaaaatt caaagccatt caatcagtga tttttttcag
       61 cacaagaaag actccttata aaaatagcag atactttgtc aattaagtgt ttaaccaggg
      121 atgataaaaa ggataggatt gaacaaaacc tgtataagct gatgaatagt ttcctcgcca
      181 gtaaaaggtt tattcgtata aagctttatc gccaaaattt atcatgacct tttttatctt
      241 caatattaaa tgaaatcaat tgttcaatca attcccagtt gattacactt ttccaaggaa
      301 ctttgatgac gttatccgta tatacgtagc ctgcttcttg gatatttttt tcaagggcac
      361 gaatcgctgc tttttccggt gcgatagaaa aatgattttt agcaaaacta aatgcaataa
      421 taaaggtacc atggtcagta aacataggtt ggttccattt tacagttgtg tctagctggg
      481 gatacgtttc tgttatctta gcaaaaaggt ctttgatctt ttgttgatgt tctttttctt
      541 tgatctgatt gatgatcggc tgaaattttt ccattctaaa acgcctccat taagtggttt
      601 gatttatttt accagaaaaa caatagttat gtataaaaaa cgaacaaaac acaagtaatg
      661 ttgtaaccgc tatatttata tgataagatg tctcaaataa taaaatagaa aagagacata
      721 aaaatgattg acttggcaaa gaaattctta cacgaaaata tatattttga gttgtatcat
      781 catcgagcca tttatacaaa tgaagatgct ttggtaataa agaaagaaca aggatttaca
      841 ggtacagaga ccaaaagctt attcttaaaa gataaaaaag ataataacta cgtatatctg
      901 acttttacta cgaaacaaac ggatttcaaa gcattaggga agatcgtcgg taaaaggctt
      961 tctattgttt catcagaacg catggaagaa caaacgggac aaaagcaggg cgctgtttct
     1021 ccgtttggtt atgaaaaaca gattccgatt attattgatg aagagctctt atcaaatgaa
     1081 aaacttgttt ttgcaccagg tagaccagat caaacgatgg tcatccaaac ggttgactta
     1141 aacaaaatca tccagctact tgagatgccg atatttttga tgccggcaaa cgaaaaaaac
     1201 aattaaccaa gtaaaatagt gcatacttgg aggtgaagca acatgataag ttatccatta
     1261 ttagaaagaa atgcaacgat tggggttact gcaccatcct caggcgtatc agaggaacac
     1321 cattcgttac tcgaatcagc tttaaagaga atggaagaaa aatctttcca agtagttact
     1381 ggaaagacgc tttggacgca agataaggaa aaatcaggtc cagcaagaaa acgagcagat
     1441 gaattcaatc aaatgatgca agatgaaaat atccaaatga tctttccgcc ttggggagga
     1501 gaattattga ttgagattct cgattttgtc gattttgatg ctctacaacc aaaatggata
     1561 gtaggttatt cagatattag tacgttgcta cttgctgtca cattaaaaac agggatagcc
     1621 actgcacatg gaaccaacct tattgattta agaggagagt acttggatga aacgacagca
     1681 atgtgggaaa gtgtactgaa aacaagagaa ggagaatcga ttctacagca accttctgaa
     1741 aagtatcaaa aagagtggcc ccatgagcat tcatcatctt ctgttttcca tctcactgaa
     1801 ccaacaaatt ggcaatcagt cagcggagat gcagtaagga tgaagggacg attattaggt
     1861 ggctgtattg atacgatcag tcatttaatc ggtacccctt atggagatgt ccaatctttt
     1921 aaaagaaagt ataatgatgg aaagccgatc ttgtggtttc tagaaaattg tgagctatcg
     1981 tcagttgatt taagaagatc acttgttcag atgaaactgg caggatggtt tgatggcagc
     2041 tcggggatca tgttcggtag aagtgcagca gaggatttgt tagattataa aatgaaggat
     2101 gtatatgaag atctgtcaaa ggaattaaaa ctaccgatct tgtatgatat tgattgtggt
     2161 catgtaccgc cacaactgac attgatcaat ggtgcctatg cagaagtagc ggtagaagat
     2221 agaaaagcga ctgttttgca aacgtttatt taaaaaattt tttcctgcct tggagtttat
     2281 caaagaccaa caaatctaaa tagaaaagaa aatgaaagaa gctgacagta ttttgccagc
     2341 ttctttttgt atgatctatt agaaatttta attatccata actatcacat tgatcagctg
     2401 tttcacatca tttggattac ctgtaggaat agctactaca gatgcattat caataatttc
     2461 ttgagttgtt tctctaatga acgctggata agatgtttga tctgcaggca tttgccaatg
     2521 tgaaggaact ggataactag tgattgtccc gtcatggtta cttgaataca ctaaaactcc
     2581 ctggtaactg tatttcccag tgagcataac cacatctttc ggatagactg cactccctgc
     2641 atcataaggg gcgataggtg ttcctgcagg ataatgaata acatgtagtt cgaaatcacc
     2701 cgatgtatct ctaaaagtat tacctctaat tgctagccaa acacgtgcgt actcaatttg
     2761 tagatcagaa tatcctgcta acggatctat tgactgagaa gactgtgagg aagttgtgtc
     2821 tattgttgaa gaagaaatat ttgtctcaac tgtactacta gatgtagaag aagtattggg
     2881 ctctgagact ttactgcttg ttgaagatgt attcgtttca gtagaagctg atttctgagt
     2941 acttgatttt tcagctgaac tggtttgttt gattgaactt ttgtctgtgg tcgcagatgg
     3001 atcactattt tctttactag gagacgtaca tcctacaagg aataaagaga ccaacaataa
     3061 aataaaacct aactttttca tataaaatac ctcattattt ttttaatacc agtatagcat
     3121 aatcctgaat ttttgaatcc gtaaaacctt gtct
//
LOCUS       DAYYME010000208         3093 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000208 DAYYME010000000
VERSION     DAYYME010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.213"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(81..1016)
                     /gene="murB"
                     /locus_tag="ACHBFW_002785"
     CDS             complement(81..1016)
                     /gene="murB"
                     /locus_tag="ACHBFW_002785"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFM9319034.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1089..2009)
                     /locus_tag="ACHBFW_002786"
     CDS             complement(1089..2009)
                     /locus_tag="ACHBFW_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9319035.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2122..2874
                     /locus_tag="ACHBFW_002787"
     CDS             2122..2874
                     /locus_tag="ACHBFW_002787"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFM9319036.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          872 a          629 c          537 g         1055 t
ORIGIN      
        1 gccatttgcg taaacaacca caaaaaagag caggacggcc accgtcctgc tcttttactt
       61 attttaagtt ccattactta ttaattggct aaacttctgc ttgtttctga tattgcttgt
      121 ttttctccaa tgatccgcac ttctgtttct aaagacacat caaatttttc tttgattact
      181 tcttggatgt gcgcaatcaa ttcaatataa tctgtcgctg tcgcatgatc gatattgacg
      241 atgaatccag catgtttttc agagacttgt gcaccgcccc atttcaggcc ttgcaatcct
      301 gcttgttgga tcagctgacc tgtatagtgt cctactgggc gcttaaagac acttccgcat
      361 gaagggtatt ctaatggctg ttttgattga cgcaattcgg tcaactcatc cattcttgct
      421 ttgattgctt cataatcccc tgtttgcaaa ctgaatcgag cttccaatac gatagcacgt
      481 aattcttgga ctttactgtg acgataagaa aataccattt cgtcttttgt caaagttttc
      541 aatgttccat cttcaagcaa aagatccact tcagcaaaaa catctttgat ctctccgcca
      601 tacgcacctg cattcatata aaccgctcca ccgacacttc ccgggatgcc gcaagcaaat
      661 tcaaatccag tcaatgattc tgccaaagct gcataagtcg tatcaatcag tgaggcacct
      721 gcttccgcaa tgaccatcgt tcctttgaca tgaatctgtt tcatttcagt cagcatgatg
      781 actacaccgc gaatcccgcc atcttgaacg atcagattgc ttgcatttcc taataccatc
      841 caaggtatat catggattcg gcaataatcg atgatttgct ttacttcttc ttgttttttc
      901 gggaatgcaa ggacatcagc tggtccacct gttttcgtaa acgtatagtt catcaatggt
      961 tcatcaaaca atatagtaat cgtggctaag ctttcaacta tttcttcttt attcactgag
     1021 gattttcctt tcttttctca aacgtcctca ttattctatc acgcttaata caatcttgcc
     1081 agtcttgttc aaagttgatg acggtagtga tctttaagca cttcctcaca taattcatga
     1141 cttcttacaa cttctttttg tttaagcaat tctgtttccg atttacctaa acggatctga
     1201 gaaaagaagg cctctaccat tggctcaaac ccgcgtttga ctagcgtgtt tgtccagtcg
     1261 ccaaattctt tcactttttt tccatcagca tcttgaatga caagctgggt cagatcgttt
     1321 acttcataag taccattttt acttgttact tgatattttt ctatattagc gccacttgct
     1381 aaatccatcg ttaaaatagc cgtttgattt tgtgtttcta attgtaaaat ggcatattcc
     1441 aaaaattcct ttgtttctct tatctggtat tttgttctca aaatgggttc atctaataaa
     1501 tacacagctg tatcaataag atgtaaaaac aaatcataaa ggacgaactc tgtcgtttct
     1561 tttgccgcta ttcgattttt ttgcaggtgg atcaaccgct tatctgggat acgtttcaat
     1621 tcttctacca taggagcaaa gcgccggttg aaaccgatca tcaaaattat ttggttctta
     1681 tcagctaatt cttgcaattc acgaacttca tttagatgga cactcagtgg tttgtcaata
     1741 taaacatgga taccattttc tagacatttt ttggctaatt cataatgtac ttttgttgct
     1801 gcgtggatca tacatgcttc gattttttct tctaaaagct catcgatcgt ttcatataga
     1861 tgttggaacc cgtactcttt ttgaattctt tcttttgttt ctttatttct tgttgcaaaa
     1921 taaaaatcaa gtgttccttg ttttttcgca taagttggta aatatgcttt ttctgcgata
     1981 ttccctacac cgattacacc aatcttcatc tactttcctc ctggatcatt tagttacttt
     2041 catcttacac catttttttc taaaatgagg tacaatagta tcgcaaaggt aaggaaatta
     2101 attcgaagga gtgtgctttt attgaaattt atctcttgga acgtaaacgg gcttcgtgcc
     2161 atcgtaaata aaaatttttt agaagttttt catgagttag acgccgattt tttctgtctg
     2221 caggaaacga aactccaagc aggacaaatt gatttagatc ttccaggtta ttatcaatat
     2281 tggaattacg cggaacgaaa aggctattcc ggcacagcga tttttgctaa aaaaccagca
     2341 ttgaatgcca cttacggaat gggtatcgat atccatgata cagaaggccg tctgattact
     2401 ttggaatatt ccgatttctt tcttgtaacc tgttacacgc caaattctca aagcgaatta
     2461 aaacgtttag attaccgatt agaatgggaa gaagcctttt ataattattt ggaaaatcta
     2521 aaaaaacaaa aaccagtcat cgtgtgtggg gatctaaatg tcgctcatca aaagattgat
     2581 ttgaaaaact ggaaaacgaa tcaaaaaaat gctggattca ctccagaaga aagagcggct
     2641 ctttctcgac tgttggataa cgggtttatc gatacattcc gttattttta tccaacccag
     2701 gaaggtgtct actcttggtg gaactaccga ttcaattcaa ggaaaaacaa tgctggttgg
     2761 cggatcgatt actttttgac gagcaaagat ttagagccag gactagctga tgcaaagatc
     2821 catacggaca ttatgggcag tgatcattgt ccagtagaat tagatttgaa ataaagaggt
     2881 tgtgaaaaag tcgtttagtt tcgagtatta agaaaaattt tgtgaaaatg gctttccaca
     2941 ttttgcataa atttggcata ataccgagaa actggctttt gaacactgtt tatcgagaga
     3001 ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat
     3061 atttttcgtt tggttgggct tattaccgca gat
//
LOCUS       DAYYME010000209         3080 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000209 DAYYME010000000
VERSION     DAYYME010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.214"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..805
                     /gene="fabI"
                     /locus_tag="ACHBFW_002788"
     CDS             50..805
                     /gene="fabI"
                     /locus_tag="ACHBFW_002788"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFM9319037.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(915..1967)
                     /locus_tag="ACHBFW_002789"
     CDS             complement(915..1967)
                     /locus_tag="ACHBFW_002789"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFM9319038.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            2093..3013
                     /locus_tag="ACHBFW_002790"
     CDS             2093..3013
                     /locus_tag="ACHBFW_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFM9319039.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          896 a          621 c          589 g          974 t
ORIGIN      
        1 tattttttta cgactatcaa atttttatca gaaacaaagg agagtcatta tgtcatttct
       61 agaaggaaaa aaaatcgtca ttatgggcgt cgctaacaaa aaaagcattg cttggggttg
      121 tgctgaagca atgttgaaac aaggggcaga aattatctat acttaccaaa atgaacgaat
      181 gaaaaaatca ttagtcaaat tggtcggcga agaagcattc actgtagaat gtgatgtttc
      241 tagcgacgaa agcatcgaaa aagcaatgaa tgccatcggt gaatatgctg gggagatcca
      301 cggccttgtc catgcagttg cttacgccaa caaagaagaa ttgtccggaa atgtgtctga
      361 tatttccaga gacggctatc aattagctca agatatcagc agctactctt taattgctgt
      421 atcaaaatac gcacaaccgt ttcttgcacc aaacagcggg atcgttagca tgacttatct
      481 aggttcagaa cgcgcaatcc cgaattataa catgatgggt attgcgaaag cttctcttga
      541 agctgctatc cgttatttgg cagctgagtt ctcgcctaaa ggaattcggg tcaatggaat
      601 ctcagctggg gcaatcaaaa cattagctgt cacaggtgtt aaagactatc aaaaactgat
      661 ccagttatct gaatctcgta cgcctgatca gcaaggtgtg acgatcgaag aagtcggtaa
      721 tgcttgtgct ttcttaatca gcccactttc cagcggaatc atcggtgata ttatctacgt
      781 tgacaaaggt gtccatcttt cataaacttt tgttgcattc tttaaagtaa aacatataaa
      841 aatcaggaaa aaacttttta aagtctcttc ctgatttttt ctttttacgt ttttttcaac
      901 tcattacttt gaaattattc cacgacttca atccagcctt ctggtgcttc tatgtctcca
      961 aattgaatac ctgtcaattc atcatacaac cgttttgtta ctggtcctac ttccgtttca
     1021 ctataaaata catgaagatt gttattgtga taaatcccac cgattggaga aataacagcc
     1081 gctgtaccac atgcaccagc ttctgcaaat tgatctagat catcaatgaa aatatctcct
     1141 tcaattgctt gcagtcctag tcgttcttca gctaactgca acaaggaata tttcgtgata
     1201 cttggtaaga tagagggcga ttgcggagtg atgaattttc cgtcttttgt aattccaaag
     1261 aaatttgctg cacctacttc ttcgattttc gtatgtgtgg ctggatcaag ataaacacaa
     1321 tctgagtaat ccagttcttt tgcttctgtt cctggaagta aactcgctgc gtaatttccg
     1381 ccgacttttg ctgcaccagt ccccttagga gcagcacgat cgtaatcaga tacaataaaa
     1441 ttcgttggtt ttagtccacc tttgaaatac gctcctactg gcgtacagaa gacgacaaat
     1501 aaatattctg gagctggatg gacaccaatg ttttcaccga ccccaatcag taaagggcgc
     1561 aagtataatg tagcgcctgt tccatagggc ggcacaaaat cttcatttgc ttttaccact
     1621 tgcttgacag catcaacgaa catcttttca gggaccattg gcatcagcaa tcgtctagca
     1681 ctttgattca tccgtcgact attttgttca ggtcgaaaga gattgacctt gccgtctttt
     1741 cgacgataag cttttagacc ttcaaaacat tgctgaccat aatgaagcgc tggagagcct
     1801 tcatgaatcg tcaggtaatt atcttctgtc aattctcctt cttcccattg tccgtctttc
     1861 catttggcaa taaaacgcca aggagttttg atataagaaa aacctagatt gttccaatcg
     1921 atttgcatat acttaccacc ttcgttattt tctccacatt ttatcattat ccatattatt
     1981 tgtcatttta ttttcagctt ttttccttca ttatagttat ttttgctatt tccttttttg
     2041 aaaacttctt ggtacaatac agaaagaatc aaattttaga aaagaggatt ttatgttttc
     2101 attattagca actgccggta cgacagactc ttctggcgag acattgacag atcaagctgt
     2161 cagcaaagta aatgcgtttc agcggttttg ggacgggatt gattgggacg ccattttggc
     2221 tgcctttatc caaaaaagtt tatatctgat ttttttgatc gtcctttttt ggattcttca
     2281 ccgtgtggga aaatatctga ttgataagtc ttttaatcag tattctaaaa aagccattct
     2341 aaacgagagc cgcttaaaga ctttacatag tcttttaaat actatttttc attataccct
     2401 tggtttcttt tttatctatg caattttatc tgctatcggt gtgccgatcg gatcactttt
     2461 ggctggtgcc ggaattgcag gtgtggcaat cggtcttggt gcccaagggt tcatgagtga
     2521 tgttattacc ggatttttta ttatcatgga acaacagatg gatgttgggg actacatcaa
     2581 gctagcaaac ttaacgattg aaggaaccgt cgtatctgtt ggtatccgta cccttcagct
     2641 gaaagcaacg gatggtactg tccatttcat cccgaatcga aacatcacca cgatcagcaa
     2701 tctctctcgt gcaaatatgc aagtactttt agatattcgg attgttcctg aagaaggata
     2761 tgacaaaatc tatgagatca ttgatcgagt aaatcgtaca ttagctgaaa aataccaaga
     2821 agagctccaa actgaacctt ctatctttgg attagtggat cttggaaaca gcaattttgc
     2881 gattcgtacg gtctgctacg cattgaacgg aaaacaatat gccttaaaag aagagttctt
     2941 gagctcctat gtcaaagaat tgacagcttc tggtttcacg attccgaaca gtccaattgc
     3001 tgtcggaaaa taatttggac aaacgatgca acgaatctca ctaaaaagag cgtgggacaa
     3061 aagtaaagaa cacttttgtc
//
LOCUS       DAYYME010000210         3074 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000210 DAYYME010000000
VERSION     DAYYME010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.215"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(594..1985)
                     /locus_tag="ACHBFW_002791"
     CDS             complement(594..1985)
                     /locus_tag="ACHBFW_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFM9319040.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(2198..2584)
                     /locus_tag="ACHBFW_002792"
     CDS             complement(2198..2584)
                     /locus_tag="ACHBFW_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFM9319041.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2640..>3074)
                     /locus_tag="ACHBFW_002793"
     CDS             complement(2640..>3074)
                     /locus_tag="ACHBFW_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFM9319042.1"
                     /translation="ELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTHRLTES
                     TWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDRRLDIE
                     AGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
BASE COUNT          932 a          659 c          526 g          957 t
ORIGIN      
        1 atctgttcta acattctcaa ctttgtcagt ttaccatagg aaaatgggaa aatctacgaa
       61 agctctcaac agaatcgtcc tacttaaata cgcttttctt taataataaa taagttggtt
      121 gtttttgtta tttgcgcttt cacttttcgg aataaataat gtgtatttat atggaataaa
      181 taaaaaaaga ggtcgtgact aaaaacaagt cacaacctct ggtaaacggt gttctcctga
      241 caactcctcg gtcacaagac acaatattca acaaacatga gaagctattt tttgaaattg
      301 ctctttgtga ctcagagtga aacggcaggc tcacgacctc tttcttaaac ggtgttcaaa
      361 agcttgcatc tgcggtaata agctcaacca aacgtaaaat atgaggaact atttttgttt
      421 ggctgttctt attacttgat gcagggcagc tttttcacaa cctcttattc acggtgtttc
      481 agaagtagct tctcggaaat aagccgaaat ttcacaaaaa tttgagaaac tatttttagg
      541 aaacttcttc ttatttctta aagctgacca cttctatcac aacctctttt tgactatgca
      601 ttcacatcga tcactttaga atcatcattg atgacatcgc gatctaaatc tttgatgcgt
      661 gcacttgtca cgataccagc taccatgcta ccgctgacgt ttactgctgt acgtgccata
      721 tcgattaatg gttcaacaga gataactaac ccaacgattg ctactggcaa gttcattgcc
      781 cctaaaacga tcagtgaagc aaatgttgcg cccccaccga ctccagctac accaaaagaa
      841 ctgattgtta caattgccac aagcatcaaa ataaattcca aactaaagac attcactcca
      901 gctgctggtg cgacgatcgt tgccaacata gctggataaa cgccagcaca gccgttttgt
      961 ccaatagaca aaccaaaact tgctgagaaa ttagctgttg catcatctac tcctaaagct
     1021 tttgtctgag tctcgatatt caatggcaaa gctccagcac ttgaacgtga agtaaacgca
     1081 aagctcaata ccggaaaggc ttttttgaaa tagttgattg gattgacttt tgcaccaatc
     1141 aaaatcagtg agtggacaat caaaacagca agcaatgcta cgtaagaagc aacgacaaat
     1201 ttacctaaat tgacgatagc tgcaaaatca cttgttgcca aaacgtttgt catcaaagca
     1261 aatactccat aaggtgtcaa acgcaaaaca agcgtcacga tccgcataat gattttatac
     1321 agactgtcga ctaaatctga aaagaatgct gcttcttttg gcgcttttct tttcacgcct
     1381 agataagcga ttccgacaaa tgccgcgaaa ataacgactc caatcgtact tgttgcacga
     1441 ctacctgcta aatcagcaaa aacgttttta gggataaagg ataaaatctg ctgtggaatc
     1501 gataagtttt caactgtttc ctgacgtgtt gccagttcag agattcgtgc tgtttctgca
     1561 gctccttgtg tgaatttagc tccttggagt ccgaacagca ttgttgaacc aattccgatc
     1621 aatgctgcaa tagctgttgt tccaagtaaa gaagtcaata cagtcgcact aattttccct
     1681 agcttttcag aaactttcat ttttgtgaat gcaccaatga tcgaaataaa cactaaaggc
     1741 ataactagca tttgcaagaa tgatacatat ccttgaccta caatcccgat ccattccatt
     1801 gattgagtaa cgactttgtc gtctacacca aagatcaatt gaagcagacc gccaaagacg
     1861 ataccaactc ctaatgctgt aaagacgcgt gttgaaaatt ttgtatggcg cttttgcata
     1921 cgatagaaca catataataa tgccgcaaaa actagaacca ctaaaactgt gatgagtgtt
     1981 gtcatttttc tcctcctcat attattaatg acaagtccat cgaaggaagt aaaggttccc
     2041 gagaatgatt cttcaaccat cctcctgaaa aaattttttt cagtgacttt tatattttag
     2101 acgattttta tcgaaatgac attctttttg cttacaatta gtaataaaaa aacatacctg
     2161 tgatagcttt aatctatcgc aggtatgttt ttactgtcta ttcttgtttt tctgattgaa
     2221 aattcaattt taccccaggc tgatctaagg cgatctctgt cacctcataa gacattcggc
     2281 gtacgatatc gaataacggt gctactagct cgtccacttc ttcttgtgtg acatcatttt
     2341 gaccttcaat tttccgattg aggatatgat tgatctgact cacacttcca gataaaacat
     2401 atgtatcaaa cactaaacgg aactctaagc gtgctcctac gatgctatgt tctttcggat
     2461 agttttcgat cgtttctttt aaaggcgaga agcctacttt caaatctgtt tctacttctt
     2521 ggttcggcaa tctcatatca taatgaaatg cttctacaac ttctttttgt cttctgattt
     2581 ccatataatt ttatcactcc tttatgaaaa ttataacata tctcctttga aaggggatac
     2641 tactttttaa tgatgctctg caaggtaagg atttcattca gatttcctac cacataatct
     2701 gccgggatct cacccagaaa atgatcgatg tcgtaaagac aagtagcgac tcctgcattt
     2761 ttccctgctt cgatatctaa acgacgatcg ccgatcatca tcgtgtctgt tctttctaaa
     2821 tgaaaagtat cgatcaaata atttaaagat gctggatcag gtttacgagg aaagtcctga
     2881 tcgatcccta ctacttcctc aatcagatgt gccaatcgat gttcttttaa caggccccat
     2941 gttgattctg tcaaacggtg ggtcaggatg aaatgacgac cgccgttgtc ttttagcatt
     3001 tccagtactt gtttcgtttc tggaaatgga cgggaaactt ccgtactttc tttttcatat
     3061 cggtgaaata attc
//
LOCUS       DAYYME010000211         3028 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000211 DAYYME010000000
VERSION     DAYYME010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.216"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1258)
                     /locus_tag="ACHBFW_002794"
     CDS             complement(<1..1258)
                     /locus_tag="ACHBFW_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9319043.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNV"
     gene            complement(1322..1681)
                     /locus_tag="ACHBFW_002795"
                     /pseudo
     CDS             complement(1322..1681)
                     /locus_tag="ACHBFW_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            complement(1859..2179)
                     /locus_tag="ACHBFW_002796"
                     /pseudo
     CDS             complement(1859..2179)
                     /locus_tag="ACHBFW_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2295..>3028)
                     /locus_tag="ACHBFW_002797"
     CDS             complement(2295..>3028)
                     /locus_tag="ACHBFW_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319044.1"
                     /translation="LCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLKNGKSDLTHS
                     IKKMNSLRVAKAIVGMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPHEDYKIIMNY
                     YLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQYEGIGFTLID
                     LKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDL
                     LIRWQVITNFLMELKKYSNTQTKFS"
BASE COUNT          940 a          564 c          403 g         1121 t
ORIGIN      
        1 caacattctt ttgtctaaaa tatctatcat atttctcaaa aagctgctgc ttatcagtgt
       61 ctttcttcca cgtttgataa agatactcta taagttcgtt gatattaaaa acttcgtcta
      121 ctttttccaa atcatccatt tgaatatatt ttaattcatt ctgtcttttc ataaccttca
      181 tgccgttgaa attcacccat tgtggtaaat gatacatctg aatataaagt tcttccaaat
      241 catctaagaa ataaaaggca ttggcacagg tcaattgaat gatatttttc aaattgccaa
      301 cattttcact aaagcgatag tttaataaaa aatctaataa ttgttgagat atcagtattt
      361 tctttgctag tctttcttct tctgatttaa aaaatatctt gattaactgt tttctttcat
      421 aaagagaacg actgtctaat gttggaagtg ttagctttac aggtattctt ctcaacaaag
      481 ttgataatag tttatcctca ggtggttctg atgttgcaaa tataatccag caacttgatg
      541 tgtaccagtt ttcattatca cccatccgat gaaatttcct cgtatccata aactgaaaaa
      601 gtttctcctg acattcattg ctcaatgcat gaatttcatc taaaaataag actccattat
      661 tagctaattc tgctaaaccc tttcttccat cttcggctcc tgtatatgca ccttttacat
      721 gcccaaaaaa attggacaag aaaagttctg gattattggc atattcacta caattaactg
      781 aaatgaaaga tgccttctct tctatataat tattggccaa caaatcctta tacacataat
      841 ttgctaaata gctttttcca gttccagtct cgccatgaag taaaatgggt aagccatttg
      901 gcggatagta gattgctgct ttaatttgag caattacatt tcgcagactt ccttgacttc
      961 cgataaccct ttctattcca accctaaaag agctatcata ccatttttcc gcttcctctg
     1021 aagagctaaa aatttgttga tttggaactt tctccggtaa agaaaagaag attactggac
     1081 gagtattgat ttttccaaat tctccatctt ttacgaattc attcaaatat tgagagacaa
     1141 tattacgact aacatttaat ttttcagata ttttctgtgc tgtattgttc ccccatttat
     1201 ttttaatctc agagttctct atgacataat tgacaatgcg ttcttttaat tcttccatca
     1261 cagcacctcc tcgacactat tcgaattttg ccgacagtat ttttccataa atatgacttg
     1321 ctaaaaaggc tttgtttaga tcattcaatt ggaacgttgc ttgttttgct tgatccgcta
     1381 agacgttgaa aagatactct ggcgaaatcg cataccccaa aatatcaacg attgttgcaa
     1441 tcagatcttc ttttgattct tcatctgaaa gtaattcacg atacaacatc gtttgtttgc
     1501 ttactgtgtc atattcctct aaggattcat ctgctaaaag aaccacttgt tccaacaaac
     1561 ggtcggataa atatttatag aaaatcagcc ccaataaata atttttatat tctgacgcat
     1621 ccattttact acggagatta tctgccgcac taaataactt ctgatttaat tctgcaccca
     1681 aaaagataca ctcctatctc ttatttaaag ttgctatata ccctcttatt ttagcatact
     1741 cagttttaga aaaagcaaaa aaataaagaa acttattctt gataacaagc tacgtgacgt
     1801 atatccctaa cacaaaaata gccatatcct ttggtttgga tacggctatt tgatattact
     1861 cattcaatta tcaaatcaca gtaggtgtga atctcatcgg gatcggcaat atctacttct
     1921 gattaggatc aaaggtgatt ttctcaataa aggtgtgata caaagcttta acttgcttct
     1981 tctcactctg gataagaaag caatccaaac tagtcaatat ctgttggaca tctttgactt
     2041 gaatcggttc cccttgttgt tgaaagatcc ctaagatttc attctctcga atttgtaact
     2101 gtcttcgttt ttcagtcaat tcatttaaac gatcttttaa catcggaaac aattcgggac
     2161 tatcttccaa agcagcttcc tgtttttgct ctaatagtta gattttgcgt accattttct
     2221 tcttttattt tccttcgatt gctgatttta atatagtcta aaggaaattt atatttattg
     2281 tattctttat aacttcaaga gaacttcgtt tgagtattac tgtatttttt taattccata
     2341 agaaaattag ttattacctg ccacctaatc agcaaatctg aacctgagtc aatataatta
     2401 atattatttt ccatctccaa cattgtaagt actgttggtg ttaatatagg gactttgtac
     2461 aattgtattt ttttgatttt atcatctgca caaaggagat tttttaaatc taatccttct
     2521 tttatttcaa aagttgaatt ttttaaatct ataagagtaa aaccaattcc ttcatactga
     2581 atttctgaaa atcgaatttt tctcccagtt gatagatcaa ggggcagaaa accttttatt
     2641 acattgtgag tgtcaccatc atttacaatg tcattaggaa ttttgatttt agaattctgt
     2701 ggagcaccta ttggaagata ataattcatt ataattttgt agtcttcatg aggaaataaa
     2761 gtacttttag gattgctaag atattctcgt accgaatcta agaatggctt atccattgaa
     2821 tcttcatccc catttaaact aaaaaacatt cctacaattg cttttgcaac tctaagtgaa
     2881 ttcattttct ttattgaatg tgttaaatca gacttaccat tctttaaact tgtattgtta
     2941 taaaggaaat ttttattgtc ttctgcaaat ctaatatatt cctcaacata ctcactacca
     3001 aaaaaactat tacatttact acataacg
//
LOCUS       DAYYME010000212         3012 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000212 DAYYME010000000
VERSION     DAYYME010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.217"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1983
                     /gene="esp"
                     /locus_tag="ACHBFW_002798"
     CDS             <1..1983
                     /gene="esp"
                     /locus_tag="ACHBFW_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFM9319045.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEGEKVEVGGTVDLTDNVTNLPTLPEGTTVTDVTPGGTIDTNTPGNYEG
                     VIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEVGGTVDLTDNVTNLPALP
                     EGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTV
                     EGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGT
                     KDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEK
                     VDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAP
                     NKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKV
                     IVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVF
                     PKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            2117..>3012
                     /locus_tag="ACHBFW_002799"
     CDS             2117..>3012
                     /locus_tag="ACHBFW_002799"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM9319046.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFS"
BASE COUNT         1195 a          429 c          632 g          756 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag gcgaaaaagt agaagttgga
      361 ggcacagtag atctaacaga taatgtaacc aacctaccaa cattaccaga aggaacaaca
      421 gtaacagatg tcactcctgg tggtacgatt gataccaaca caccaggtaa ttacgaaggg
      481 gttatcgaag taacgtatcc agatggtacg aaagatacag tgaaagttcc agtagaagtg
      541 acagacaatc gttccgatgc cgataaatat gaaccaacag tagaaggcga aaaagtagaa
      601 gttgggggca cagtagatct aacagataat gttactaact tgccagcgtt accagaagga
      661 acaacagtaa cagatgttac tcccggcggt acaattgata ccaacacacc aggtaattac
      721 gaaggtgtca ttgaagtaac gtatccagac ggtacgaaag atacggtgaa agttccagta
      781 gaagtgacag acaatcgttc tgacgccgat aaatatgaac caacagtaga aggcgaaaaa
      841 gtagaaattg gaggcacagt agatttaaca gataatgtta ctaacttgcc agcgttacca
      901 gaaggaacaa cagtaacaga tgttactccc ggcggtacaa ttgataccaa cacaccaggc
      961 aattatgaag gtgtcattga agtaacgtat ccagacggta cgaaagatac ggtgaaagtt
     1021 ccagtagaag tgacagacaa tcgttctaat gcagataaat acaatcctga aggacaaaaa
     1081 gtgactacag acttaaataa agagccagat gcatctgagg gaattaagaa caaagaagat
     1141 ttaccaaaag gaactactta tgcttggaaa gagaaagtag atgttagcac agctggaaat
     1201 aaaaaaggta ctattgtagt gacatatcca gatggatcat ctgatgaagt tgaagtagac
     1261 gttacagtga cagatactcg ttccgatgca gataaatata atcctgaagg acaaaaagtg
     1321 actacagact taaataaaga gccagatgca tctgagggaa ttaagaacaa agaagatcta
     1381 ccaaaaggaa ctacttatac ttggaaagaa aaagtagatg ttagtacagc tggaaacaaa
     1441 aaaggtactg tagtagtaac atatccagat ggatctaaag aagaagtaga agttactatt
     1501 tctgtagtag ataaaaaagc accgaataaa cctcaagttg atcctattac agagggtgac
     1561 caaattgtta ctggtaaaac tgaaccaaat gcagaggtga cagttacatt accagacgga
     1621 agtcaacacc atgggacagc tgataaaaat ggtaatttta cagttaaagt tcctaaatta
     1681 gaggcaggga caaaagtgat agtaactgca actgatgaat ctggtaatac tagtgaacct
     1741 actaatgtag ttgtttcttc caacaaaaaa gacagtggaa aaaacggtag taaaggtagt
     1801 aagactgaca atcaaggtag caacagcaat caagacaaaa atagaggcaa aagccagtcc
     1861 tcaaaagttt ttcctaagac gggagagagt gacagtaata tctttactat tagcggtggt
     1921 ttaatactgt taggaacgtt aggattatta gggtataaaa atcgaaaaaa agagaatgaa
     1981 tagaaaatca atttcttaag tcttctctga gaagaggaag aatctttttc ttcctctttt
     2041 tgctttttta tgatgggaaa taagtgttag ttaaagatga atatactttt tatagaaagg
     2101 aaaagaatgt aattaagtgg aaaaaatagt aattattggt tcaaaccatt cagggctagc
     2161 cgcagctcga tttctaaact taagcgattc aaattatgaa gttattctaa tcgataaaaa
     2221 atcagaaatt agttacttgg aatgtgggac atcgttattt ataaaaggag aagtaagtaa
     2281 tttagaagat cttttttatc aaaatagaat gacactaaat tctgagataa aaaagttatt
     2341 tctagagaca gaagttgaca aaattgatat tcaaaataaa aagattagtt gtgtaaaaaa
     2401 agatggtaca agattctctc aagaatatga caaagttata ttagctacag gttcaaagca
     2461 aattatgcta gatataccgg ggaataattt agctaatatt ttttcgataa agaattttcc
     2521 aaatgcaaaa gagattttta aagctttgga tgatagtact attaaaaaag taggaattat
     2581 tggagcagga tatattggag ttgaaattgc tgaagccata agaaaaagag ggaaagaagt
     2641 atatcttttc gatgttgcag atagagtgtt gtcgacttat tacgaccgta gtttttcaga
     2701 taaagttgaa gaaatactat caaaaaatgg aatacatctt tgtctatcag aaagtcctaa
     2761 aaaatattta ggaagaaaaa agattgaaaa aatagtcact acaagtggta cccaatactc
     2821 tatggatatg gtcgtacaag ctataggatt tctatctaat agtccaattg gggaaaagga
     2881 actcttgaga gatgttagtg gagcatatct aactaatgaa taccagcaaa caaatataaa
     2941 agatatatat gctattgggg attgtgctac aacttttttt acgtccatta atagaagtag
     3001 tgttgatttt tc
//
LOCUS       DAYYME010000213         2963 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000213 DAYYME010000000
VERSION     DAYYME010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.218"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            255..686
                     /locus_tag="ACHBFW_002800"
     CDS             255..686
                     /locus_tag="ACHBFW_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9319047.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            693..926
                     /locus_tag="ACHBFW_002801"
     CDS             693..926
                     /locus_tag="ACHBFW_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9319048.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            1350..1550
                     /locus_tag="ACHBFW_002802"
     CDS             1350..1550
                     /locus_tag="ACHBFW_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM9319049.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            1634..2827
                     /locus_tag="ACHBFW_002803"
     CDS             1634..2827
                     /locus_tag="ACHBFW_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9319050.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
BASE COUNT          826 a          695 c          796 g          646 t
ORIGIN      
        1 cacagggcta ttccggttat gagtatgtac tataccatgt aaatcagcag catagcaaag
       61 cattttctcg cccggtaggt ttgcgacctg ccgggcgatt tttgtcgtct tttgcggccc
      121 ggctgtcccc aaaaaaattt tttgagaaat tttcaaaaag gaggtattta gcgggtagct
      181 ggcggccttg tcaaagtctt gttagcggaa agggagagaa ccacctaagt cccccacaga
      241 aagggggtga gaagatggat gctatccccc gcgactatga agcgcggtgc atgttcgatg
      301 ctttttgcaa gacggtactg cgtaacgagg ccaaaagcta cttggctgaa atgaagcgcc
      361 gccgtgaccg tgaagtgtca ctaagctctt tgtcacaggc agatttggac aagctctgta
      421 cagtagatca ctaccccagc gacagcttta cgttttcgtc gcatggctat gacctacata
      481 tcaacaatga gcttgttgcc gaagcctttg ccgcgctgcc cagcatggag cagagtattt
      541 taatcctgca ctgtgttctg gatatggcag atggtgagat cggcggcctt gtgggaatgt
      601 cccggagtgc cgttcagcgg cacaggacaa ataccttatc ggagctgcgg aaacagctaa
      661 aggcgttgat gccgaaggga ggttagacgc ggatgaagaa cccgaactac aagggcattg
      721 agcttttgcc cctttcggtg atcgaggccg cccgcgctgg ggatgccgga gccgtagagc
      781 gcgttttgca gtattacagc ggttacatga acaagctgtg tacccggacg ctctatgacg
      841 aaaacggcta tccgcatgtg tgtctggacg aggccatgaa gcgccgtctg gaaatcaagc
      901 tcatccacgc tatcgtcacg aactgacgaa ccggccccgg cagcatgaaa cggcttaccc
      961 tttccccgtt tcggtgtttt cccggtggcc tgatcgcacc ttgataaata tgcccgcaag
     1021 ataacggcgg cgcatcatag ggcatacgga cacattctgt ctgtgccgag ccggacggct
     1081 ggtgcgccat gatacttttc cctacgggaa aagcggagcg aggaacacca cgccgcaatg
     1141 tgagcgtggc ggcttgcggg cgacgacgta caggcaggac tatgatactc ccttatagcc
     1201 acagtccgag cgttaaaagc gtcgcaggca atgggtaggg ccgcgtgaga accacgcggg
     1261 ggtgaaagtc ccgtggagct gtgccagcag ccgtccgtct atgcccctaa atcacacaaa
     1321 cctttgaatt tgtgaaaggg ggctgcatta tgaaccatga tgtgcctatt tgggaaaaat
     1381 acacgctgac gattgaggaa gcgtctaaat atttccgtat cggagaaaac aaattgcgta
     1441 agctggccga ggaaaatccg gcctctggct gggtcattat gaatggcaac cgcatccaga
     1501 tcaaacgcaa gcagtttgag aaaataattg acactttgga cacaatctaa tagtgaaaaa
     1561 gggccgcaga tatgttataa taatttataa gcatatatgc ggctctttcc gtcatggaaa
     1621 ggagcatgac gagatgtcag aaaaaagacg agacagcaaa aatcgcgttt tgcggtctgg
     1681 agagagccag agaaaagacg gacgatatgc ttacaaatat gttgatacct ttggaaaacc
     1741 gcagtttgtt tactcatgga agttagtgcc tacggataag accccggcag gtaaacggga
     1801 ggatatttca ctccgggaaa aggagaaaga aatccagaaa gaccttgacg acgggataga
     1861 caccatcggc aagaaaatga ccgtctgcca gctctacgcc aagcagatca ggcaccgggg
     1921 caatgtgaag cacaacacca aactgggccg ggaacggctg atgcgtattc tggaacaaga
     1981 tcggctgggg agctgcccga ttgacagtgt gaagctgtcc gacgcaaagg aatgggcgct
     2041 tcggatgaaa gaaaagggcc tttcttataa gaccatcaat aacgacaagc gttccctgaa
     2101 agcggctttt tacaccgcca tacaggacga ttgcatccga aaaaacccgt ttgacttcca
     2161 gcttagtgac gttctggacg acgatacgga accaaaggtg cctcttacac cagcacagga
     2221 agaaagtttt ctgtccttta ttcaaggtga taaggtttac caaaagcatt atgacgcaat
     2281 cgttattctg ctggggacag ggcttcgcat ttcggaactg tgcggactga cggacaaaga
     2341 tttggatttt gaaaaccggg taatcatcgt gagccaccag cttttgagaa ataccggcgt
     2401 cggttactat attgacgaac ccaagaccca aagcggcgtc agaaaaatcc ccatgaatga
     2461 ggaagtgtat caggcgttcc agcgagtgat aaaaaaccgc aaaggtgcaa agccgttcat
     2521 tattgacggg tatgccaatt tcctgtttct caaacagaac ggctatccca tgacggcggt
     2581 agattatggc gggatgtttg gcaggcttgt gaagaagtac aacaagagcc atgaagaagc
     2641 gttgccgaaa acgacgacgc cccatgctat gcgtcataca ttctgcaccc ggttagccaa
     2701 tgcgggaatg aaccccaaag ccttgcagta cattatggga cattccaata tcaccatgac
     2761 gctgaactat tatgcacacg ccacattcga ttccgcaagg gcagaaatgg aacggctggc
     2821 agcctgacac aaggccggtt ttactactcc atttactact tttgaggggg aaatggtgag
     2881 aggtaataag aacatttgta agtatctaac aatatcaaaa atgccggaaa agcccggaaa
     2941 cacgggctat accgacattc aag
//
LOCUS       DAYYME010000214         2941 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000214 DAYYME010000000
VERSION     DAYYME010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.219"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            104..2164
                     /locus_tag="ACHBFW_002804"
     CDS             104..2164
                     /locus_tag="ACHBFW_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFM9319051.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2247..2816
                     /locus_tag="ACHBFW_002805"
     CDS             2247..2816
                     /locus_tag="ACHBFW_002805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9319052.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
BASE COUNT         1055 a          529 c          567 g          790 t
ORIGIN      
        1 tctcctaata gtccatcttc ttttgatttt atttatataa tgaaatcatc ttatgaaaat
       61 tactacttca tgtttagaca agagtaggga ggactaagaa taaatgtgtt caaaaagacg
      121 ttcaaaacaa ctaaataaac acgcttgtcg tttagataag gaacggtcgt gttctgaaaa
      181 acactacgtt aagtttatga tgcgcacgat gctgatcagt ttatctgtag gtgtctatat
      241 gcatgtaccc gttcaggcaa cgacacaaac catcaaggtc gtacgtacct ctgtttctga
      301 gaccactgat tcccctatca ctattactca tactttcact gatgcaaatg gaaaacagct
      361 acttcctgca ttgacttcct ataataaagt cagttatcct ccaacgataa accactactg
      421 gttaattcct aacaaaacat tatggacatc ggaaaccgga agcagtgtgc cgctttctat
      481 cttttccgat atgactgatc ctacaaaagg tgatcagtta aaacagatca ctgatatttt
      541 gaatcatgct ttgacagata taaaggacgg tggaagcgca caagtttctt atgtatatga
      601 aaaagattat tcacagtttg aaagaaagaa aatcgatgtt ctggcaggga caaaagtttc
      661 gatatctgat ctggtagaaa ggctgaagaa cgcagatgga acacaaggag atatgtccaa
      721 agtaatttca aacaatggga atatcgtgga tacgactaat acaggaacat ctactatttc
      781 cttgtcctat tttgatccaa ttgctttaaa aacaatgact gcatctgctg tgatccatgt
      841 tcgtgctgat caaacagcta tcaaaggaaa agatcttcac ttaagaacag gagagagtta
      901 cgcccctaaa gatcttgttg aatatgtaac aaaaagcgat ggaacttcag gaaataaaga
      961 agaaatcaaa atgaacggga cagtagatac tacaactccg ggagaatatc ccgttgtatt
     1021 aacttatata gactcgaaaa catcaaatgt ggcagaaaca acagctgtag tttatgtaga
     1081 agcatctgct gcttcatcga ctgtcatcac tcattttata gatagtcaaa caaataaaga
     1141 acttcaagca gaaacacata tgagtagtgt aaatcgtatt cctgatttgc gcctacctgc
     1201 tgaattttct gatcgagtgc caaatataga tttatcgact tatgaacaaa atgggaaagt
     1261 cacttctatt tctgattgga tgcaagcaaa cggattagac agaaaaagtc actggtcgga
     1321 gatccttgaa gtacttcact cagaactgat gaataatagg aagcaatcag gaggggaaat
     1381 tacctttact tatgtttata taccagacaa tcgttcatta gaaggtcatg atcttattgt
     1441 tcatccaaac gaaaaaataa acaaagaaga gttgatccaa tcagcaacaa attccttagg
     1501 taaacccgta ccgttaaagg atgtatcggt tacaatcaat gaagtaccat tggaggatgg
     1561 ttatttgacc aaacaaacag gtgattttac gattatgtat agttatcatg acccgtattc
     1621 actccaagaa ctccatgctc agtcactatt acatgtttta ccattaaaca aacagataga
     1681 tcaaaaagaa caaaaaagac aagaagaaag cttgtcagaa aaaaaacaac aaatggctca
     1741 tgcagtttct ccaacaggga aagtaaaaca aacaagaagc ctagagttag caagaatatc
     1801 tagaaaaatc tcagaatcta ataaaacgat aaggcaagaa gctcatttgg ccccacaagt
     1861 ttctggattg gaaaatgaaa ttttagagcc taaaataaaa caacaatcag aaaaagaatt
     1921 tgttgctaaa cagtcggaag atcagaagga atcagatgac cagaaggaaa cgaagaaaaa
     1981 acgcttgtca actattgatt ccaccgatga gcatctgcag ccgcaagtac cacttcctag
     2041 tccgggaaca cccgcacctg gtggaggtat tccttcttct gctttaggag atttcatgcg
     2101 aggtatggca ggaacatggg tatacgctaa tcgaaatgaa tttgacgcag atgactggtt
     2161 ctgatttata tctctatctg ttatagtcaa ataagtagct gagtcatatg ttatttggca
     2221 taatggagac gaggagggat agaaaaatgt tagataaaac gttaccttac gcagaaatct
     2281 ggatgacaag accacttcaa caaaatgttc caatcacgcc tttagcggaa ggttttgaat
     2341 ttcgatttta tcaaaaagaa gatgaatctg cttgggcgga gattgagact tccgttgggg
     2401 aatttcaaac gacagatgaa gcacttgagt attttcaaca aaactttgct ccttatcctg
     2461 aagaacttga aagaaggatg ttttttgtag agacaaaaaa aggagaaaaa gtagcaacct
     2521 gtaccgcttg gcgaaaaaaa tcaagagagc aagtgtatcc ggtttttcat tggttagcag
     2581 tgaaatcaga ttaccaagga aaagggctcg caaaagcatt gactgccaga gtcttgcaag
     2641 aatttcctac cctccatacg gacggaccaa tctatcttca cacacagaca tggagccatc
     2701 aggcgattgc tctttataaa aaaatgggtt ttacttttat tgctgaaaat ctagacggca
     2761 cacctaatcc agattatgaa aaagttatgc aggttttaag taaattagat aaataacgaa
     2821 atggtgaaat aagtcctcag tattgggata aaaaacgaaa tagtcattcc tcatatctta
     2881 cttttttgag ttaatactat cggataacta atggcatgcc atacattcaa aaaagaaggg
     2941 g
//
LOCUS       DAYYME010000215         2899 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000215 DAYYME010000000
VERSION     DAYYME010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.220"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            573..>2899
                     /gene="esp"
                     /locus_tag="ACHBFW_002806"
     CDS             573..>2899
                     /gene="esp"
                     /locus_tag="ACHBFW_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFM9319053.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1054 a          441 c          567 g          837 t
ORIGIN      
        1 ttataaagaa aaagaatcaa taatttaatg aggctgtgac aaatgtcatt ttttgtttcg
       61 ctaaaaacga gtgaaaaaat gttgataact ccaatcattc gtttaagatt ctaggaatca
      121 ttcagaaatg ctgtgaaacc gatactttat aattataaag aaggtgattt caaattccga
      181 agaaggaatt tgaagtcacc tttttactta tgtcacagtc tcattcagta atgcgggata
      241 aggctcctta ttccgttatt tgaatactct gaagtttaat ctaagaaagg gtaatatttt
      301 gaaaacatgc ttcatcgcat agaaatgaac ttaatttcag aaagaataca tacatatctt
      361 tttatatgtt ttcagctgct gaatggttaa aaagcacaaa tatccgaaaa aaacatgaag
      421 aaatgtactt tttttgttgt aatgttcatg aaattctcta agtaaattag ttaacataag
      481 gttagtaaga ttcctattct gtgaataata cgtatcagat ttatctttta ttcttactag
      541 gaaagtaatt attaagggag ttgatttgaa taatggttag caagaataat aagagagtat
      601 ttctagaaaa aacaaagaaa cgagtattga agtacagtat caaaaaatta agtgttgggg
      661 ttgcatctgt tttgattggt gttggtcttg tcttgggaac aactgaacta gttaaagcac
      721 aagatgaaat ttctccaagc agcccacttg aaacagcgat aagctccgta caagtaggtg
      781 acaaagtagc ttcggggaat acttttcagg agaatcctgg ttatacgaaa aattataact
      841 tttcggattt acaatttagt cctcaagaac taactggaga cacactaaaa ggcaatacaa
      901 ttggatttga agtttacgga aaacataata ttgcagcatc aactaaaaac tgggaaatcc
      961 gtcttcaatt agatgaacga ttagctaaat atgtagaaaa aattcaggtc gatccaaaga
     1021 aaggaattgg atcttctaga cggacctttg taagaattaa tgattcgctg ggtagaccta
     1081 caaatatttg gaaggtcaat tatattcgtg caagtgatgg attatttgct ggggcagaaa
     1141 caaccgatac gcaaaccgct cctaatgggg taattacgtt tgaaaagagc ctagatgaaa
     1201 tttttaaaga gataggcata gacaatctca agaccgatcg tttaatgtat cgaatctact
     1261 tggtaagtca tcaagacgat gataaaattg taccgggtat tgatagtacg ggctactttt
     1321 taacagattc agatgatttc tataatagct tagatgtatc tgaaaataat cctgaccagt
     1381 ttaaacatgg atcagtaagt gcaaaatatg agaaacctaa tactcaaaca aaagatggtt
     1441 caggatccac aggtgctaat ggagctataa tcttagatca taagttaacg aaaaattata
     1501 atttctcata ttcatcttcc gcaaagggca cgccttggta tgctaattac aaaattgatg
     1561 aacgattagt tccatatgta gcgggtatac agatgcacat ggttcaggct gacaaagtaa
     1621 catatgatgt ttcgtttgaa tccggaacaa aagtagctga tttagcaatc gagcgacggg
     1681 aaggtcatga aaattatggc ataggttcaa taactaataa tgatttaact aaacttatcg
     1741 attttgctaa tgcaagtcca cgtccagtcg ttattcgata tgttttacaa ttgactaagc
     1801 cattagatga aatcttagaa gatatgaaag caacagcaca agttgaagaa aataaaccat
     1861 ttggtgaaga tttcatcttt gattcttggt tgtcggatac gaataaaaaa ttaatccaga
     1921 acacttatgg aacaggttat tattatttgc aagatattga tggtgatgga aaccctgacg
     1981 ataaagaaga gagcggagac acgaatccat atatcgggaa acctgaatta gaagaagtat
     2041 atgatgttga cacaacagtt aaggggaaag tattcatcca cgagttagcg ggaacaggtc
     2101 acaaagccca acttgttgat aaagaaggta ctgtattagc agaaaaaact atcgctccaa
     2161 atgaaaaaga tggggctcca atttcagata ctgtagaatt tgaatttacg ggtgtagatt
     2221 caagtaaatt aatcgcgaaa gatgaattaa aaatccaaat cgtttctcca ggttttgata
     2281 aaccagaaga aggttcaacc gttattaagg aatcaccaaa agcggttgat aaacaaaccg
     2341 tggtagttgg atttaaacca gatgctaaag aatcaattcg gaataataaa aacttacctg
     2401 aagatgcaga gtattcatgg aaaacagagc ctgatacttc taacgttact gatagtacga
     2461 aaggtattgt aactgttaag atcggaaatc gaactttcga cgtggatgta gagtttgctg
     2521 taaaagcttc tcaagctatg gaaaatgatg caacatacgt acctataaca acaactccag
     2581 aaacgacagt tcaaagtgga aaacctacat ttgataaacc agatgttcct ttagctaaag
     2641 atgccttttc aatttcagat gtctataata agtacttcgg taatgcaagt gttgatgcaa
     2701 acactggtat tgttacattc actccagcta aaggtgtagg agaatcggag ccgattactg
     2761 gaacaattcc tattaaaatt gtttaccaag atggttctgt aggcacgacc gatttagcag
     2821 taactgtaag taaagatatt tatgaaaatc caggagaaaa cattcctgca ggctaccaca
     2881 aagtaacctt caccgcagg
//
LOCUS       DAYYME010000216         2832 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000216 DAYYME010000000
VERSION     DAYYME010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.221"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            18..632
                     /locus_tag="ACHBFW_002807"
                     /pseudo
     CDS             18..632
                     /locus_tag="ACHBFW_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(1002..2003)
                     /locus_tag="ACHBFW_002808"
     CDS             complement(1002..2003)
                     /locus_tag="ACHBFW_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFM9319054.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(2162..2428)
                     /locus_tag="ACHBFW_002809"
     CDS             complement(2162..2428)
                     /locus_tag="ACHBFW_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9319055.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(2418..2774)
                     /locus_tag="ACHBFW_002810"
     CDS             complement(2418..2774)
                     /locus_tag="ACHBFW_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM9319056.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          903 a          508 c          382 g         1039 t
ORIGIN      
        1 aaaagtaggg aataaatttg aaaattgctt atattagagt ttcatccatc gatcaaaatg
       61 aacaacgtca aattgaagaa atgaaaaagt ttggcgctga aaaaattttt atcgaaaaac
      121 aatctggagc gacgattagt catcggccga ttttccagga agctttagat tttgtcagag
      181 agcaagatat ttttattgta gaagccattg atcgacttgg acgcaattat gacgagatta
      241 tcgattcagt gaattatcta aagaagaaga atgtgcgact tattatcacc agtttaccga
      301 tcatggctga agcaattgga aatcctctcc tagataaatt catcaaagac ttaatcattt
      361 aaatctcagc catgattgct gaacaagaac gtactgaatc gaaacgacgc caggcacaag
      421 gaattaaaat cgctaaagca aacggcgtct ataaaggtcg tcctaagtta tacagtgcag
      481 acaccaaaga tcctcaacgt cgccttgtct ataggagtat cgttcaggat cttgagaatg
      541 gggttgctat ctcaaagatt gcaacagact acaatgtgac taggcaaaca atttaccgaa
      601 taaaaaaaga aatcgaccaa ctaattgtat gatttctttt ttgaatcacg taattattaa
      661 ttagctttgt catcaataca gacaatcatt ttagattcat ctagctaagt tgattacatt
      721 tatgttaaaa agaatttagt taacataaat ataattgatg cataaatcgt ccttatttaa
      781 aggcatttaa cattgacaac ttagtataat gtatcttatg taaactaata agatttgaat
      841 tttattttat acacactcga taatagatca attttatggt cccccgcatt tacgattgca
      901 caagagaaac ataaagtcga cctattagca tttcttttca attcaaacat taaaaaagtt
      961 tatacctctg ctactaaaaa atgaaattat ctatttaaaa tttaaaacgg taaatctgat
     1021 tcatccaaat tggaaaatgg atcagcctgt attggagctt tgtatttctc cataataaca
     1081 gatatctttt tttcattttt taaaaaatac cttttaaatt tcttatagtc ctctgaaatc
     1141 aaaatatagc gtgatggata ctcatcaaaa gaagaatcgt attcttcctt taaaattccc
     1201 gatttactta atttttctag tacatcaaat actctatcac tcaagtaact ccttaaattg
     1261 ttatcacttt cccattgaac tacttttcta taagttgaat tatcagttga aaagatagaa
     1321 ttttcatcca taaacgcata tattagcagc aatctttcat cattgtcaaa ttctattgga
     1381 tcaaaaatat ctaagttgtc atcaaaatct ttatcagttc cggtaacttt tttgttaatt
     1441 actgaaggtg tcttgtaaga aatagaatct aaatcaaagc gttttgattc taaattattt
     1501 tgtgctttct caattaaata attcactttt tttgtgagaa accttctgat attgtctttg
     1561 ctatatgttc cataaaaacc cttatcttct aaagagtccc tgtacttctt aacttctata
     1621 tactcttcag ccaaaacttg tggaactggt tcatctaaga aaaaaacccc tacctcgcct
     1681 ttgctatttt tataaaagta atcaatttct gctatagttc ccgatctata attatcaact
     1741 ggagaaccca acttatggcc aaaaatcgct aataatatat cactattttc tacaatttgt
     1801 ttattaataa cactttgccc agagctatta tttatataag taggaatcgc attcgtttcc
     1861 catcgaattg gcatcaaaat tacttttgta ttatctgtat tttctatatt ccatctatat
     1921 atggtttctt ctacttcatt cctctttgct gctatatcac ctggtgatgc tataaatact
     1981 tttattagat ttatggtttt catttattgt acctttctct ttatgtctct ttaaagtaat
     2041 ttttgtataa aatttattga cttttacact ataggacaac ttttatctat ttagtacaaa
     2101 aaggcccact aaactaattg attagttagg agcctattac aggtatattt tcaactcgct
     2161 tttaagcaaa ttctactacc agctttttgc ctaatgccct agcgatatca tcaagtttat
     2221 cgaaagatac gttatcccct ctctcaattc gagcaatagt agattgagtt gtatgtgctt
     2281 tttgtgcaag ctccgcttga gttaggcctg cttcctcccg tgcatgataa agagcaaccg
     2341 cagtttcgag ttttcttcct tcacggtcat atgcttctgc aaaatctttg tctttttgct
     2401 tttcttcaat caatttttta atcatcattt gtgaaccact cctctctaag ttcttttgca
     2461 tgtttaagtt cacttattgg tgttttctcc gtttttttag taaatccatg ggtaatgaca
     2521 taccttccat tgaccacatg gaaatagata gctctttgga tgtttgatcc taccttactt
     2581 cttaattcgt acaaattagt gtctaatttt ttcacccatt tcaaccgttg agctactagc
     2641 atcccattct tttctgtttc ttctattgta cgtaaaagct tcgcactatc tttttttggt
     2701 aatgtttcca accattctaa aaattcatca tgaccattag gtcttttaaa tgtttcaaaa
     2761 gatatttttt ccatacccaa ttatatcata tattgatttt atattatagt atataacata
     2821 aaaattcgaa tt
//
LOCUS       DAYYME010000217         2714 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000217 DAYYME010000000
VERSION     DAYYME010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.222"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            38..1045
                     /locus_tag="ACHBFW_002811"
     CDS             38..1045
                     /locus_tag="ACHBFW_002811"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFM9319057.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1057..2061
                     /gene="neuB"
                     /locus_tag="ACHBFW_002812"
     CDS             1057..2061
                     /gene="neuB"
                     /locus_tag="ACHBFW_002812"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFM9319058.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2058..>2714
                     /gene="neuC"
                     /locus_tag="ACHBFW_002813"
     CDS             2058..>2714
                     /gene="neuC"
                     /locus_tag="ACHBFW_002813"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFM9319059.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     E"
BASE COUNT         1006 a          346 c          534 g          828 t
ORIGIN      
        1 aattatagat gactataatt cataggagga gatataagtg tttaaaggaa aaacaatatt
       61 agtaactggc ggaactggtt ccattggaag tgaaattgtt cgtcagcttt taaaatatga
      121 accaaaagct atcagaattt atagtagagg tgaagaaaaa caattttata tgcaacaaga
      181 gctctcaaat cacactaatt tgagattttt aattggtgat atcagagact ctcaaaggat
      241 tgattatgca tgtcgtgatg tggatattat ttttcatgct gccgcaatga aacacgttcc
      301 agcaagtgaa tataatccta tggaagctgt aaaaacaaat gtgatgggaa ctcaaaatgt
      361 aatagatgca gcgatcaaaa ataatgtaaa acattttgta ggtatcagta cagataaagt
      421 agtgagtcct actaacacca tgggtgcgac aaagttattg agtgaaaaat taataatagc
      481 tgccaatcag tataaaggaa aaacagaaac aatattttct tgtgtaaggt ttggaaatgt
      541 catggggtca agtggatctg ttattcctct ttttagtaaa caaataaaaa acaataaaga
      601 tattactttg acagatgttg atatgacacg atttatgatg tcaataccgg atgctgcaag
      661 attagttctg tctgctgcta tgaattcaaa atttggagaa acatatgtct taaaaatgcc
      721 ggcaatcaat attttaactt tagctcaatc attaataaaa atatataatg atacacatga
      781 tgaaaaatat agaggtgaaa taaaagttgt aggtatcagg ccaggagaaa aattatatga
      841 agagctaatg actacagacg aaattgaacg ctcatatgaa aatgataaaa tgtatatcat
      901 tccaccacac tatcatgcta ctgacgaact gtacaaagat tatgaaaaaa ttaaaatcac
      961 tgcatactct tcagatgcaa cagaattaat gagtggacag gaatttatta gaatcgcaaa
     1021 agagaataat gtcgtgactt ggtaaggagt tttatgatga aatcagaagt atatgtaatt
     1081 gcagaaattg gtgtaaacca taatggaagt ctagatttag ctttagaaag tattgataaa
     1141 gctaaagaat gtggagctaa cgcggttaaa tttcaaacat ttaaaacgaa taagctagtt
     1201 tccaaaaagg ctccaatggc aaaatatcag aaggataatc ttaaagagga cagttctcaa
     1261 tttgaaatgc taaaaaaatt agaattatct caatctgatt ttattagaat aaaagattat
     1321 tgtatacaaa ataaaattga ttttttgtct actccttttg atgaagaaag tgcagacttt
     1381 ctaaaacaaa ttggtgtaca aggatttaaa attggctcag gagatttaac aaatttacct
     1441 tttcttagaa aaatagatat gtatgggttg ccagttttat tatctacagg tatggctgat
     1501 attcatgagg tcagtgaggc tgtagattgt ttcatagata gtccagttac tattttacat
     1561 tgtacttcaa attatccggc tagttctgaa gatattaatt tattggcgat taacacatta
     1621 aaagaaatgt tcgggatacc cgttgggtac tccgatcatt cattgggata tgatgttggt
     1681 atttgtgctg ttgcgatggg tgctaaggta attgagaaac attttacctt ggataaggaa
     1741 ctacctggtc cagatcataa agcttcttta gatcctcagg aatttgcaga ttttgtaaag
     1801 catattagaa acactgaaat atttttaggt gatggtgtta agcgagcaat gccttctgaa
     1861 cttagtacaa aagaagtatc aagaaaaagc ctagtggtca ctgagaattt aaatgtggga
     1921 gatattctta cagagaaatc tcttacaata aaacgtcctg gaaatggaat agctcctaag
     1981 tttttatatc aatatattgg caaaaaagtt aaaagaccaa taagtgcaga tagtgttctg
     2041 cttgaggaag atgttgaatg aaaaaaatac ttatagtaag tggaacacga gctgattatg
     2101 gaatatataa acctgttgct gagaatttac tacaagataa ttgggatgta tcatttgttg
     2161 tttctggaat gcatttatcc cataaatatg gatttactaa agaattgatc ttagctgacg
     2221 gatttagaat tagtggagaa gttcaaagtt tgtttttaga gaacaataag ggaaacatgg
     2281 ctcggtctgt ggcattagag ataaatggtt tcacaaatat atttgaacaa actagccctg
     2341 attttgtatt attactaggg gatagaggag aaatgttggc agctgcgata gcttgtttgt
     2401 atcttggaat caaaacagca catattcacg gaggcgaagt gtctggtact gtagatgaat
     2461 caataagaca tgccataacg aaaatagcat ctgttcattt tgctgctacg gaaaaaagca
     2521 aagagcgttt aataaaaatg ggggaagatt cttggagagt ttttcccgtc ggagctccta
     2581 gaatagatac tattttaaat aatagattac ctagttttag agatataaaa gaaaaatatc
     2641 atctaaaatt tgaaaagaaa aaatacgttt tgcaagtatt tcatccagtt gtaaccgaac
     2701 tagaaaatat agaa
//
LOCUS       DAYYME010000218         2677 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000218 DAYYME010000000
VERSION     DAYYME010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.223"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            700..786
                     /locus_tag="ACHBFW_002814"
     CDS             700..786
                     /locus_tag="ACHBFW_002814"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM9319060.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1088..1312)
                     /locus_tag="ACHBFW_002815"
     CDS             complement(1088..1312)
                     /locus_tag="ACHBFW_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319061.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1679..1963
                     /locus_tag="ACHBFW_002816"
     CDS             1679..1963
                     /locus_tag="ACHBFW_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319062.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1966..2382
                     /locus_tag="ACHBFW_002817"
     CDS             1966..2382
                     /locus_tag="ACHBFW_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319063.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2519..>2677)
                     /locus_tag="ACHBFW_002818"
     CDS             complement(2519..>2677)
                     /locus_tag="ACHBFW_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM9319064.1"
                     /translation="NPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKI
                     SLRNWLEE"
BASE COUNT          883 a          498 c          430 g          866 t
ORIGIN      
        1 cgttttttca tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca
       61 actcgtaaat aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg
      121 ttagtactca gataaaaagt tgttagtact cagataaaaa gttgttagta ctcagataaa
      181 aagttgttag tactcagata aaaagttgtt agtacccttc tgtatccgtt gggagagtaa
      241 ggctcaactg ccccctaaaa agggttctaa aaaatcttta aaaaataaat aaaataaacg
      301 cgcgcgcgcg agcaaaattt cccgccaaaa aatcacccac tttttaaaat aaacaggttc
      361 gcgcatgata ttaaactgcc aatctagctc cctatggtcg ctgtaagaag gagtgatcgc
      421 tacgctctca cattttactt gcagcagtcg cctttaggct ccttcgccaa aaataattca
      481 ttagggactc ctttgtggcg tcctgagagc tcgtctaagc cgttctaaga caagattcga
      541 taaaaactat gtccttcgtt ctaaagcgcc ttacaggcgg tcagggcaag ccctaacaac
      601 cccaaggcaa taaaaaaact taggcttatt tttgcttttt tattgtataa ttgattgtcg
      661 cctatccgac agtcttgcga ctggaaggag gtgtgtttcg tgttcactaa ctttatttgc
      721 tcccttcttg ttgggctttt aagcgctttg ttcagttact ggttgaataa ccgaaacaag
      781 aagtaaaact taattaaggc gactcagccc acaaaaaaag ccccagagaa tccgacctct
      841 ctggggcttt cgtgtgtttc gtgttcactt atttgcaatc aaattataac agaaataaat
      901 atttatttca aaaatatcct acaaaagtct aaaatccgta attatttctt atatgcaaca
      961 tttacaaata aagtcgctct gagagctcgt atttttcgat ttaagacgtt ttatttagtt
     1021 tttggtgtat ttcattggtc caagctaaaa aactcttaga agcgatttta gagccattta
     1081 aacccactta attatattct ccatgaaatt cgctccatgc gtcaaaagct gcgtctgaaa
     1141 ttccgccgtc ttcgttcact agttgcatta atttttccaa cgatcgtttt ctttcgggtg
     1201 tcatattttt atattccaac aatttttcgt ctgttaaatc gaacttgatt gcttgaattg
     1261 cttgctctaa tttttctaag ttttgaaaat attcgtaagg aaatttttcc attttttctt
     1321 tctccagtct cacaattttt ctagaaataa aatcacttct ttgccgtcta tactctcaaa
     1381 aaaaatgtcg tttgaaaaac taaataaatt tttgatcgtg catttttaaa tttggttatc
     1441 gattacacgt gcagcactcc ccccccgaat tttaaagagc ataaggaaac gtgtacccac
     1501 tttctaaaaa aagtgggtag ataggctgtg cctatcacgt ttctcagaaa aaaatttgat
     1561 atcacttttt cgccatcgtg ggtagcttat gtatagcatt ttggcaaaaa acagctatac
     1621 aaatatagta tttgtatggt atacataaat tatacataat tgcaaaagga gattttttat
     1681 ggacatttta gttcggaata ttgatccaaa gtttgttgca acaatagaca aacgttgcca
     1741 agaactcacg aataaaacag gagaaaaatg gagtcgaaat aaatatctaa aagttttaat
     1801 cgaaagtgat tttgaccgtc aacttacgac ttacaaaaaa gaccaattcg atttattgct
     1861 cgataaattt attgctatcc aaacagaaaa tactcaaata ttaagcgagt atgtctacac
     1921 aaataatcaa atcattgaaa tgcttatcgg aggaatgata taaccatgag aaaaacttct
     1981 gtccaaaaac atgttacttt gtaccctagt catgttgacg ttttagattc catcatcgaa
     2041 aaaaatccag atatcaataa ctactcggaa gccattcgct ttttatgtat gagttatgaa
     2101 cccgatacca acacgaccac taccagcaat caggcaaaac taaatgctat gggaaaagaa
     2161 atttccatca tgactgaaat catcgcagaa tttgctgatt tatatctaaa agatacaggc
     2221 gttcttattg gcacaaattc taaagtatat caagaagtga aagaaaaaat cgaagctaaa
     2281 ctaaatccat caaatcgaaa tcgaccaaag aatcatcaaa aagaaaccag tcaaacagtc
     2341 acttctagaa atttcgaccc aacaaatttt ctaaaacgat aaaaaacaga taggagttat
     2401 tcctatctgt tttttattta gaagccccat acaatatgct agaatgtact agcaatctta
     2461 gtcggttgta agggacttgt ctacttgctt aatgtaaggg gggcgagttc ttttttattt
     2521 attcttctaa ccagttacgc agagaaatct ttggcaaatc tgacgttagg cttgaaaaag
     2581 taaattctaa tttggcgatt ttattgccct tttttgcttt tatttttgtg actttaagat
     2641 cattgaagta tttttttagt tctcgtaatg caggatt
//
LOCUS       DAYYME010000219         2633 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000219 DAYYME010000000
VERSION     DAYYME010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.224"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..626
                     /locus_tag="ACHBFW_002819"
     CDS             120..626
                     /locus_tag="ACHBFW_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM9319065.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            643..1542
                     /locus_tag="ACHBFW_002820"
     CDS             643..1542
                     /locus_tag="ACHBFW_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFM9319066.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1629..2513
                     /locus_tag="ACHBFW_002821"
     CDS             1629..2513
                     /locus_tag="ACHBFW_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM9319067.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT          958 a          489 c          515 g          671 t
ORIGIN      
        1 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
       61 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
      121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
      181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
      241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
      301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
      361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
      421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
      481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
      541 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
      601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
      661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
      721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
      781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
      841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
      901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
      961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
     1021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
     1081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
     1141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
     1201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
     1261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
     1321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
     1381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
     1441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
     1501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
     1561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
     1621 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
     1681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
     1741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
     1801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
     1861 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
     1921 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
     1981 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
     2041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
     2101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
     2161 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
     2221 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
     2281 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
     2341 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
     2401 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
     2461 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
     2521 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
     2581 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat ttt
//
LOCUS       DAYYME010000220         2628 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000220 DAYYME010000000
VERSION     DAYYME010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.225"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(706..2046)
                     /gene="glnA"
                     /locus_tag="ACHBFW_002822"
     CDS             complement(706..2046)
                     /gene="glnA"
                     /locus_tag="ACHBFW_002822"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFM9319068.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(2074..2466)
                     /locus_tag="ACHBFW_002823"
     CDS             complement(2074..2466)
                     /locus_tag="ACHBFW_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9319069.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          782 a          571 c          441 g          834 t
ORIGIN      
        1 taaaaaacgt agtccacagt tctcggattt ttggcatatc ttttctatta aataatatca
       61 aaaacaaaac aattccacta attttttcaa agatgacctt tttttcaaca aaagtgcctt
      121 gtagggattc attgaaggta acgaatcaca taagtaaaaa ataaggagat gcgacaaaag
      181 tcgtgcctcc ttatttctaa ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
      241 caatatccat caaacatgag aagctgtttt tgaaattatt tcttgtgatt cggagttaaa
      301 tggtaggctc acaacctctg gataaacggt attcaaaagc cagtttctcg gtattaagcc
      361 aaatttatgc aaaaatgtag aaagccattt tcacaaaatt tttcttaata ctcgaaacta
      421 aacgtctttt tcaaaacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa
      481 gttcagccaa ttcaaaaaca agaaaagctg tttttgcttg gctattctta ttacttgttg
      541 cagggcagct ttttcacaac ctctccacta aacggtgttc aatcagctga ccctcggtat
      601 tagctcaaaa aacggcaaaa tatgaggaac tatttttccg ttagtttctc taatactcag
      661 gtcaaaacgc tgatttcaca gcctctaatt cacggtattc ctgttttaat aaagttctag
      721 atattgttct ctttcccact cggaaacagt ttgtctgaat gccgcccatt cgagacgttt
      781 tgcttcaaca aaatttgaga agatatgttg tcctaatgcg tcaatcatta cattatcctt
      841 acgcaattct ttgatagcat tatggatagt tgaaggtaag tcctggatat gtgcagcttc
      901 tctttcttcc tcatccatta cataaatgtt acggtcaaca gctgctggcg gtgtgatctg
      961 attacggata ccgtctagtc ctgcttgcaa caatactgcc attgccaagt atgggtttgc
     1021 agatggatca actgaccgca attccaaacg tgtggataaa ccgcgagatt ctggtacacg
     1081 tactaatggc gaacgattac ggccactcca tgcgacataa acaggcgctt cataacctgg
     1141 aacgagacgt ttataagaat tgactgttgg attgcaaacg gctgtgtagg cacgagcatg
     1201 tttcagcaaa ccacctaaga aatgataagc tgtttcactt agttccaatt caccgtcttt
     1261 atcatagaaa acatttcctt catcattgaa caaagacata ttgcaatgca taccagaacc
     1321 attgatccca aataacggtt tcggcatgaa agtcgcgtga agtccatgct tgcgtgcaat
     1381 cgttttgaca acgagcttga aggtttggat attatcacaa gcatctacca catctgcata
     1441 tttaaagtca atttcatgct gtcctggcgc tacttcatga tgtgaagctt ccacttcaaa
     1501 gcctagactt tctaattcca acacgatgtc acggcggcaa ttttcaccta aatctgttgg
     1561 tgcaaaatca aaataaccac ctttatcatt caaggtcgtt gtgatcccac cgtcttcgtc
     1621 taatttaaat aagaagaatt ctggttcagg tcctaaattg aaagaggtaa aaccaaggtc
     1681 gcgcatatct ttcaatgctc gtttcaaatt cccgcgagga tcgccggcaa atggtgtccc
     1741 atccggatta tagatatcac aaattaaacg agcaactttt ccatgagcac tttcccacgg
     1801 gaagatcatc caagtggaga gatctggata caaatacata tcgctttctt caattcgaac
     1861 aaatccttca atcgaagaac cgtcaaacat cattttattt tctaatactt tgtcgatctg
     1921 gctcactgga acttctacat ttttgatcgt acctaagatg tctgtgaaca tcaatcgtaa
     1981 aaatcgtacg ttttcttcct caatgattcg tttgatttct tcacctgtta tctgtttctt
     2041 taccatggta tccctcttcc ttttttaatt tattcagtca aagaattgtc tttgcaatcc
     2101 ttgcgactga taatgacctg ttttgcttaa cccaccttgc gccaaaatct catcacgaaa
     2161 aatttgtcga acgtcatgat ctgtcaacgg cttcttgttt tttgcttctt gttttttcgc
     2221 ctgtgccatt tcgtatacac gtttgatccc agccatattc aatccctctg aaagatagtc
     2281 tttaatctca agcaacatat caatatcatt cagcgaatac atacgtcggt ttccttcgct
     2341 tcgttccgga tgaactaaat cttgttcttc ataatagcga atttgccgag cagacaaatc
     2401 agtcagcttc ataactgtac caattggaaa tacggacata gatcggcgca attccttttc
     2461 tcccatcaga cgtccccttt cgttttataa gttggctaaa gtgtaccaat taaaaaaaat
     2521 attgtcaact gcatatgtta ggttttctaa catgagatct ctatttttta taaaataaca
     2581 aaaaaataag aacacaaaaa aggtatctag ttgattttcg taccagat
//
LOCUS       DAYYME010000221         2608 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000221 DAYYME010000000
VERSION     DAYYME010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.226"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(193..759)
                     /locus_tag="ACHBFW_002824"
     CDS             complement(193..759)
                     /locus_tag="ACHBFW_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9319070.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(759..1715)
                     /locus_tag="ACHBFW_002825"
     CDS             complement(759..1715)
                     /locus_tag="ACHBFW_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFM9319071.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1715..2371)
                     /locus_tag="ACHBFW_002826"
     CDS             complement(1715..2371)
                     /locus_tag="ACHBFW_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM9319072.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT          801 a          536 c          483 g          788 t
ORIGIN      
        1 aaagctgtcc tgcatcaagt aataagaaca gccaatcaaa aacagcttct catgtttttg
       61 atttggttgg acttattacc gcagatataa gcttttgagc accgtgaata aagaggttat
      121 gaaatcaacg ctctaacctg agtattagag aaaaaaacgc aaaacatatc atttatgttt
      181 tgcgtttttt atttatcttt ttttctcaat acaatataaa ataggcggct ggttcttttg
      241 gttgataaac cgataattca gcacattata tgtttcctgt ggcagtttgg aacaaaaaga
      301 atcaaccata tccagttctt tttctccacc ttcatgacca tagtagacga caaggatcaa
      361 tcgtccgcga ggcacaagtc gtttcaatat ttcttccata gctgtgcgtg tagtctctgg
      421 catagtgatg attgctttat cactttttgg gagataccct agattgaaaa tccctgcttt
      481 gagattttgt tgttcagcta tcacttctcc taacgtttcg tggccttgta gaaacaatga
      541 gacacgttct tctaactgac gctgttctag cctttcacga gtagcatcga ttgcttgttg
      601 ctggatatca aaactataga catgaccagt ttttccgatg tgttctgcta gaaaaaccgt
      661 atcgtggcca ttgcccatcg ttgcatctac gacgtgatcc cctggttgca agatttcttg
      721 cagtaatgta tggctgaaat gcaaagcagt ttgcaacatc agacgctcac ctttttccgt
      781 acatcatatt ttccttggaa gctatctctg cgaagaagtt cttcatcaat tgcattcaag
      841 acttcccatt ttttcaagct ccacattggt ccaatcaagg aatcccacgg cgcatcccct
      901 gtcaaacgat ggatgatgat ctcttttggt atcatttcca gctggtcgca aatgacattg
      961 acatagtcag gacggctcat taattgaagg cgaccttcat gatagtcccg caacattctc
     1021 gttttgcgca tcagatgcat caaatggagc ttgattcctt ggatatctga atctaaaatg
     1081 gtgcgtctga cattttcttt catcatttct aaactttcgc ctggtaatcc gttgatcaaa
     1141 tgcgtacaaa cacggatatt atgtttgcgt aatttagcca cgccatctaa ataagtttga
     1201 taatcatgtg ctcggttgat cagttttgaa gtttcttcaa aagttgtttg caatccaagt
     1261 tctacccata aatacatccg ctcgttcagt tcagcaaggt aatcaactac ttcatctggc
     1321 agacaatccg gtcgtgtacc gactgataat cctacgacac ctggtaaatt gactacttgc
     1381 tcaaatcgtt ctttgattat ttcaactggc gcatgggtgt ttgtaaaatt ttggaaataa
     1441 acgatgtatt gttggacatg aggccatttt tgatgcatca tgtcgatttc tttctggaat
     1501 tggattggaa gagggtcttc aggtgcaacg atcatgtctc ccgaaccaga tacactgcaa
     1561 aaagtacaac cgccatgggc aacagtccca tctcgattcg gacagtcaaa tcctccatcg
     1621 atcggcacct tgaaaatttt cccgccaaac tcatttcgta aagcgtaatt ccaagaatga
     1681 tatcttttat ttccttctga ataaggaaaa gtcattaaaa gtccttcttt ctgtaagagt
     1741 tatctatttg ttttcttttt tctctataca ctggataagt caggcacagc caagaaagac
     1801 tgagtaagta accgccgatg atatcagtag gaaaatgaac gcccagataa atgcggctta
     1861 tcccgacact tactataata aacatcatca gcaattgaag caatgtgcgt aaggttcttt
     1921 tttgaacaaa agctggaagc agaaatagta aagaaccgta aagaatcata ctgcctgctg
     1981 catgtccact agggaaactg taacttgttt caactactaa gtgcgccaat gaaggacgct
     2041 ctcgcatgaa aaatagtttg atcaccgggt tgatgattcc agcaattaca ataaatccag
     2101 aaacgaacca aaatgtttct gccctttttt tcttatacca catccataaa gccgcacaag
     2161 caaataaaat gatgacggta agaggattac cgaatttagt cataaagagt tggtagctgt
     2221 tcagatcagg ataagaagct ctgacaatgc ctgtaaacca ctgatcgaaa ccagaaagcc
     2281 acgacagatg gaactttacg atccagccca aaatgaaaaa caggacaaga aagaagctac
     2341 cagccacctg aaatgatgtt ttatatttca tacaattcct cctttgaagc aatgacttat
     2401 tatagcattt ttggtccagc gaaaaaaacg gtaaagagat aaaaccaatg tatgattcct
     2461 ctttgaataa ccaatattct tataaaaaaa ataactgtaa tcggaaagac attttttggt
     2521 aatgcaaatg aaatgttagg gaaataaaat cagtgttttt cgaaatcctt ttaaacaaga
     2581 acgtagacgt aggaagaact tgaaaatg
//
LOCUS       DAYYME010000222         2579 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000222 DAYYME010000000
VERSION     DAYYME010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.227"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..504
                     /locus_tag="ACHBFW_002827"
     CDS             112..504
                     /locus_tag="ACHBFW_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319073.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(677..880)
                     /locus_tag="ACHBFW_002828"
                     /pseudo
     CDS             complement(677..880)
                     /locus_tag="ACHBFW_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            846..1043
                     /locus_tag="ACHBFW_002829"
     CDS             846..1043
                     /locus_tag="ACHBFW_002829"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319074.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(1086..1661)
                     /locus_tag="ACHBFW_002830"
     CDS             complement(1086..1661)
                     /locus_tag="ACHBFW_002830"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM9319075.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1816..2342)
                     /locus_tag="ACHBFW_002831"
                     /pseudo
     CDS             complement(1816..2342)
                     /locus_tag="ACHBFW_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          820 a          442 c          405 g          912 t
ORIGIN      
        1 taagtaaaaa atataggtga ttttttttga cttcatattt ataataaaga cacgtaaatc
       61 aattattttt cattttactt taactaataa tgtttatttg gagggattat tatgttagat
      121 gatagattga cgtgttctaa ttgtggtaca ttattttact atgaggaagt tagaaatatt
      181 gtaaaacata gaaaaaaaga aatgcctata cgttgtccaa attgcaatga agttattaag
      241 cgaaagttgt ctcatggtta ttttgtcagc tacgaactat atgaagaaag taaaataaat
      301 ctgaaaaaaa aaccaaaata ctataaaaat ttgaaaactg gagctattat aactgaaaaa
      361 gaatttaaaa atttagtgac tgaagaagaa agcaggtttt ataatttgtc tggtaatatt
      421 tgccctggat ttatggatca gattattgaa aatgagtcaa ttgaatcaat tgaaaactat
      481 gttcctgttg acttaccaaa atagaaaaaa taatcctgtt tttacagtga attattttaa
      541 ttttgatttt caataaaaat atattacaat aataatcgta atagctaatg aaacaatttc
      601 tcaatctttt taccactcca aacagggagc gtcaataatt ttgtgtaaat aactcgtcct
      661 tctgcaaaat aatgggttac tcagtaaaca ttgaagctaa tgtatccgtc acttgttgga
      721 agcctttatg gcttctattt aagaattttt gattgtatgt atcaaagatg gatactagga
      781 aacgctctag tgattcttca ttttgaaact gctcttttct gcggctgtat ttcttgattt
      841 gtttattgaa agattcgatt gtagaaccag aaggaatgga gaacactttt gaactttaca
      901 acaaaactac tgttgataaa ttaattccaa ttttagttcc aactgattta aatggtcatg
      961 atgttttact tcgttttaac ggatttttag gtgaggatta tagataccta aaaatttctg
     1021 atcctcaacc tttaacagaa taataaaaaa cgtgtaatca attatttgat tacacgtttt
     1081 ttatttcaat cttcgttgta gtagtggttg cgacataccg aaaggtaatc ttcattacct
     1141 cctactacaa tctgttcacc tgaataaact ggaataccat caacaatacg agcattcatt
     1201 acagcctttt tattgcaaaa atggcaaata gttttaattt cctcaatttt atcagcaaat
     1261 gtcaataatg cttcggatcc cttgaagaga ttattttgaa aatcattctt caatccatag
     1321 caaattactg gtacttcctc attcattacg ataagtgata gatcaataat attctctcta
     1381 cttaaaaatt gagactcatc gaccaaaata caatcaactt ttccgtactg atcaataatc
     1441 tccactaatt ctccgtacac atccatttca gaagtaattg caatagctgg tttcgaaaca
     1501 cctatacgac tctcaacttt tccaaatcct gatcgagtat ctattgcgct tgtcattatg
     1561 ataactctct tcccttgttc ttcataatta tgagccactt tcagtagctc aatactcttt
     1621 ccactattca ttgttccata tctgtaaaat aattgtgcca ttaaatttat tcccttcttt
     1681 tacaattcaa ttattcctta attccatctg cttttatgca gctgctcgtt tattccttga
     1741 tatgtctata acatctacta tccctaaaac caatttataa gccattagag atggtattcc
     1801 tgaacatctc tcatattgtt ttttgagtgg tatatcatct gaaaaactga aggaatcatg
     1861 aaaatagcga catacttatc atttagagtt ctaactttaa atgcatttac ttcttcagtc
     1921 agcactttag tcatgttgga catggtttgg ggcgtgtagt gatgcccata cattttctca
     1981 atcaagtctg cgatttccga catggtaata ccttttcgga agaggtgaat gacggtctcc
     2041 tctaatgtgt cattcgtccg tctataagca ggaacagtct gttgcttgaa ctcgccattg
     2101 cggtcgcgcg gaatctggag atgaagttcc ccatactcgg tcttgaccgt acggtcatag
     2161 gagccgttac gagaattacc cgtgttaaaa caaatgcgat cgtacttttc gtaatcgagg
     2221 aaagccgtta actccgtttt gagaagcgta ttgacagctt tttccaaatg gacacggaat
     2281 aatccattca aatcgccttt gttcgctaga gtctttagga tttctgtagt aaaatcgttc
     2341 atatggaagt cctcttttct gtgaatgtgt tgtggtaact ctattctaca gaagggactt
     2401 ccttttttct atttacacaa aatattttac actctcaaca aaagtatctt cgattaatac
     2461 ttgctcaagt tatgtgcagc tacgaaaaag taggtaccga ggtactttct ttttcgcagc
     2521 tttttttgga cttatgagaa gtacattcgc tggaagaata cgatataaaa cgagctttt
//
LOCUS       DAYYME010000223         2517 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000223 DAYYME010000000
VERSION     DAYYME010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.228"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..118
                     /locus_tag="ACHBFW_002832"
     CDS             <1..118
                     /locus_tag="ACHBFW_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFM9319076.1"
                     /translation="KETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            152..787
                     /locus_tag="ACHBFW_002833"
     CDS             152..787
                     /locus_tag="ACHBFW_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFM9319077.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            900..1535
                     /locus_tag="ACHBFW_002834"
     CDS             900..1535
                     /locus_tag="ACHBFW_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319078.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(1528..1662)
                     /locus_tag="ACHBFW_002835"
     CDS             complement(1528..1662)
                     /locus_tag="ACHBFW_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319079.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            1834..>2517
                     /locus_tag="ACHBFW_002836"
     CDS             1834..>2517
                     /locus_tag="ACHBFW_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFM9319080.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINK"
BASE COUNT          991 a          373 c          433 g          720 t
ORIGIN      
        1 caaagaaaca caaatttcaa gtgcagatga aattaaaaag tataaagagt tgttagatga
       61 agggattatt aatcaagaag aattcgattt taagaaaaaa gaattgcttg gtttataata
      121 ttcaatatca aaagagaaag aggatctatt aatggacggt atatttggtt ttactggtat
      181 tgttttattt attataggtt taatcatgtt aattgtaaga tttatcaaaa aaacaaataa
      241 gaaaacacct atgattctcc ttattatcgg gattattttt actaccgtag gtttctcgct
      301 ttcctcatca aacgaggcaa atgacaataa caaacaggaa agctcatcat ctgatactca
      361 aactactaca tctaacagca gcaataaaag cctaactcaa tttgaagaat ttttagagac
      421 aaataatcat gactggggtg ctttcttaga tagttattat tctattactc cagctactga
      481 gcaaaataca gcttttacta aatatattag tggtaaaaca tatacttttg aaggaactgt
      541 tatcgaatca atgactacta gaatagccat tatagcagac aaagagtacg atgataaaag
      601 ttggaatgat atttctgcaa cacctaaagt ttcatatgta atttttgcga aggacttaaa
      661 caatgctgat acctttacaa agggcgataa agtaactttt acaggagaaa ttagttcaag
      721 aggatcgaac atagaaaaat cctatgctca atgggatatg attaatagta aagtaagtaa
      781 aaaataaatg aaccacgccc cactaagagt tagattgtgc acaataataa aacctataca
      841 aaagagtgcc atcaggagtc acaatgcttg taaaggaaat gacaaaatag gaggtaacta
      901 tggataatag caatgaaaaa ctaaccaaaa aagaatatct tgaattaata gttgaaggcg
      961 gtattggagc aattccttat attggtggcg ttgtgcagtc actttatttt gggtctaaaa
     1021 acgagaagcg tttcaaacgc atcgagaaat tctacaaaaa tttaaacgaa tcattagaaa
     1081 acattcgcga tcaaattcca gaagatgttt tcaacttaga gaacaaggac caactcattg
     1141 gaatattcga agggataaat gacgaggtag aaaaatcaaa ggcacaaaat aaaatagata
     1201 tgtataaaaa tctttataag aattgtttac ttcgaattaa caacgcttca tgggataacg
     1261 aggagtatta tcttcttgta ctgaatcaat taacatcgat tgagattcaa ttgttagcat
     1321 gcttaatgag tcgtggaaac gaaaatttca ctggaaatat cagcatgtct ggctattcac
     1381 aagagctaat agatggtagc ttaaatagat tatctgattt tggtctgtta gaaaaaacga
     1441 ttagctccat agtattaggt gatgtcggca aacaaaatat gggctacaaa atatctcatt
     1501 taggaattca atttattaac tcaactctta cttaagtttt ctttctaata tgacatctag
     1561 acctatcagc aatgcaacaa taaaaaacca gacataccaa taatcaacaa ataatctaaa
     1621 ctctggaagt tcagttattc ttttaaaaat cactttaatc ataataaaca cctctgattg
     1681 gatgatctaa tacacatcca attataccat taaaacgaca aatagcacat ccttgcgccg
     1741 gcaagcaaaa aggatgtgca aaaaataata aaacccaaac aatgggcttc tttatagttc
     1801 ctattgtatc agagaaagag agctgattca attatggcaa gattagtcaa acgtggaaat
     1861 agttggcaat acgaaatttc atacaaaaaa gatgacggaa aatacacgaa gataagaaaa
     1921 tcaggattta agacaaaagg agaagcaaaa gatgccgcca acgaattaga atataacttg
     1981 aacaaaggcc ttaaagggga tcgcaaaaat ctattattat cagattactt tgaggattgg
     2041 atgcaacttt ataaagaagg aacagtatct cctatcactt atagaaaata cgaagatacg
     2101 ttaatgaaca taaagaaata tatgccagcg gtattgattt ctgatttaga tagagtggga
     2161 tatcaacgct ttttaaataa gtatgctaaa gaccatgtaa aatctaccgt tattaagttt
     2221 aataaccata ttagagcgtc gttgaaagat gccgtagaag aaggattaat tccgtttgat
     2281 ccaactagaa aagcagtaat caaaggaaaa gattcattga agccaaaaga agataaatat
     2341 ttagattatg atcaatttaa atctttaatg aaactcgtag aagaaaacct ttctgcgcag
     2401 tactcttctc ctatgctcgt gttagttgct ggtgccactg gaatgcgatt tgctgaactt
     2461 ctaggattaa catgggaaga tatcgatttc gaagatcaaa tcatcactat taataaa
//
LOCUS       DAYYME010000224         2503 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000224 DAYYME010000000
VERSION     DAYYME010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.229"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2182
                     /locus_tag="ACHBFW_002837"
     CDS             <1..2182
                     /locus_tag="ACHBFW_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFM9319081.1"
                     /translation="KKESSASQQSNWSGLYFNSNEYVKYILNKTAWIKIDGEKYSPRQ
                     IVLYEKLQAHVPGLYGVSEQSLMELLGQRIVEQLDFRKNMAMFSDDEIRAILVELPKF
                     DKGEISRRLYNELIRNKKGMNPTYSTEGLSVLAKDGFFYPNKELRYADKRLPKSIEKQ
                     MRFIYVPEKASTTTIYEWLGVERFKTNLKLETFELLEDYSEDFKKEIDDIKTAVLSTI
                     DDNSKNVSSLKRIQIIPCSKISAKDVEQDGQSIVLEDYYFVESKGKFYLKLPANSIEI
                     NQLRLIDTFSSTIVDIFKQVLNLSLDLNLIELLISRDTEHKQRKITEEFGVDKWNESF
                     ELLFSENALNKKVNDFLLSNGLNEEEYPTIFEIDYSYALKEEEYQLLAIALESISKDI
                     EDLNGLSELIHLDIREFWKLKLRSYLDKKAEHYRQILYSNANKSGDEILKETFLEKID
                     RYKDYQFEISEIPNSVQVDIEKESKKRFPELSVSITDEIDIDGLYDNNVEELVTVAAI
                     TIDDFDYYIRGHQKVSSLLYFGIPSRLREDILIFVTKENDVDLNGEKSGISIEGTSTV
                     QTILEPSNSVSRKGKNTRNGERSKRDYDNRNSANDDAGETAEQIAYAELTQNQKLNVI
                     WNTKYSSKSSDRNKQPPNGIVCDMWVHDPENGNMYFEVKSSITEFEMSINEYNSMLDN
                     PDSYEVVLVNRDTKEISRHKFDELDGLKQASSYLFKFKQKRSDG"
     gene            2317..2445
                     /locus_tag="ACHBFW_002838"
     CDS             2317..2445
                     /locus_tag="ACHBFW_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM9319082.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT          956 a          328 c          481 g          738 t
ORIGIN      
        1 aaaaaaagaa tcaagtgcaa gtcagcaatc gaactggagt ggattgtact ttaattcaaa
       61 tgagtatgtg aaatacattt tgaataaaac tgcatggatt aagattgatg gagaaaaata
      121 tagcccgaga caaattgtgc tatatgaaaa gctccaggca cacgttccgg gattgtatgg
      181 agtttctgaa caaagcttaa tggaactctt ggggcaacgc attgttgaac agttggattt
      241 cagaaaaaat atggcgatgt tttcggatga tgaaataaga gctatattag ttgagttacc
      301 aaaattcgat aaaggcgaaa taagtcgcag attatataat gaacttataa gaaataaaaa
      361 aggaatgaat cctacttatt ctaccgaagg attatcagtc ttagccaaag atggcttctt
      421 ttatccaaac aaagaattaa ggtatgctga taagcgactg cctaaatcaa tagaaaaaca
      481 aatgcgtttt atttacgtgc ctgaaaaagc tagtacaact acgatatatg aatggcttgg
      541 ggttgaacgc ttcaaaacaa atttaaaatt ggaaacattt gagttattgg aagattattc
      601 tgaggatttc aaaaaagaga ttgatgatat caaaactgcg gtattatcta cgatagatga
      661 caatagtaag aatgttagtt cattaaaacg tattcagatt attccatgca gcaaaatatc
      721 tgctaaagat gttgagcaag atggtcagtc aatcgttttg gaagattatt attttgtgga
      781 aagtaaagga aaattctact taaaattacc tgctaattct attgaaatta atcaattacg
      841 tttgattgat acgttttctt caacaatcgt agacatattc aagcaggtgt taaatctaag
      901 cttagatttg aatttgattg aattgttaat ttcccgagat acggaacata aacaaagaaa
      961 aataactgaa gaattcggtg tagataagtg gaatgaatcg tttgagctat tgtttagcga
     1021 aaatgcgtta aataaaaaag ttaatgactt tttgttatca aatggactaa atgaagagga
     1081 atatcctacc atttttgaaa ttgactattc atatgcatta aaagaagaag agtatcaatt
     1141 gttggcgata gctctagaaa gcatttcaaa agatattgag gatttgaatg gattgtcaga
     1201 attgattcat ttggatattc gtgaattttg gaaactgaaa ttaagatctt atctagataa
     1261 gaaagcggaa cattaccgcc agattttata tagcaatgca aataaaagcg gtgatgaaat
     1321 actcaaagaa acattcctag aaaaaattga tagatacaaa gattatcaat ttgagatttc
     1381 tgaaattcca aattcagttc aagtcgacat tgagaaagaa agtaagaaga gatttccaga
     1441 gctaagtgtg tcaattactg atgaaattga cattgatggc ctgtatgata ataatgtcga
     1501 agagttagtc acggttgcag ctattacaat agatgatttt gattattata ttagaggtca
     1561 tcaaaaagtt tcttccttac tgtattttgg tattccgtcc cgtttaagag aagatatact
     1621 gatattcgtt actaaagaaa atgatgttga tttgaacgga gaaaaatctg gtatctctat
     1681 tgaaggaaca tcaactgttc aaaccattct tgaaccatcg aatagtgtta gtaggaaagg
     1741 taagaacaca cgaaatggag aaagaagtaa acgagattat gataatagaa actccgcaaa
     1801 tgatgatgct ggtgaaacag cagaacaaat tgcttacgct gaattgactc agaatcaaaa
     1861 acttaatgta atttggaata cgaaatattc tagtaaatct tcagatcgta ataagcagcc
     1921 accaaacgga attgtttgtg atatgtgggt tcatgaccca gagaatggga atatgtactt
     1981 tgaggtaaaa tcatcaatta cagaatttga aatgtcgatt aatgaatata attctatgtt
     2041 agataaccct gactcttacg aggtcgtttt agtcaatcgt gataccaaag agataagtcg
     2101 tcataaattt gatgaacttg atggattgaa acaggcaagt agctatcttt tcaaatttaa
     2161 gcaaaagaga agtgacggtt gagtgatttg tttccacaaa cctattcaat ctatcaactc
     2221 aaaaatcata aacaaccatt attatagtag gaagatacag agtgtactct caacgcatat
     2281 cgaattagtc gcttcattcg ttaaaggaga ataaaaatgg accaaaaaga aaaaatgaaa
     2341 gaattgatca aaaagaaaca aggtggaggc cgatccaaac aaatggaaac acctaagaat
     2401 gatcgtaaaa acatgagaaa aggccctaaa atctttaata aatagacgtg ctaaagaaag
     2461 actaagaagt gttaactttt gttattgttt cttagtcttt tta
//
LOCUS       DAYYME010000225         2492 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000225 DAYYME010000000
VERSION     DAYYME010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.230"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            189..2342
                     /locus_tag="ACHBFW_002839"
     CDS             189..2342
                     /locus_tag="ACHBFW_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFM9319083.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLVQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          857 a          427 c          547 g          661 t
ORIGIN      
        1 aaaaataagt ttatttttta atgaaaaaaa tagttggata tgtaacttta agtgattgct
       61 gtgaaacaaa aagttgattt tatcttgaaa ttaggtaaag aatttcttgc tccgcccatt
      121 ttttcttttt tattaagaag tttttgggta caatgaagca tgaatgtaaa taggcggaag
      181 gataggaaat gatgaaagat tttatgaaaa aaatattaaa tacttcttgg ttagaaaaaa
      241 taagaaaagg tgcacaagaa cctaagaaca aaacgtttcg ccggtcccat gttccttttc
      301 gcttaaattt tctttttttc attatattta cgttatttgt tgcgttgatt gctagattgg
      361 gttatttgca gatcgtgaac ggcgaagaga tggaggcgcg agtgaagtct acttctacga
      421 ttacggtcca agaaagtact cctcgcggaa tgatctacga ctccacagga aaagctttag
      481 ttacgaataa agcgaatcag gccatcacat atacgagagg aacacaagtt acggctaaag
      541 atttgcttga aatcgcaacg aagctgaact cgctgatcga tgttccggta gatgaaaatc
      601 tgacagatag agataaaaaa gatttttggc tggctgatcc caaacatttg aaagaagcca
      661 caaaaagact ttcggcaaaa gaaaagcaat tagggaacag cgaacaatat agtgcgatgg
      721 tcaataaagt cacagctgat gagattaatt taaacgagga gcaattaaaa attgctacca
      781 tctttaaacg gatgaatagt gcatatgaat taaacactgt ctatatcaaa aactctggtg
      841 ttactgatca agagttagct gttgtggcag aacgcgcttc tgaacttcca ggagtttcga
      901 ctggcacaga ctggacacga gaatataatg cagcaagttc actgaaaagt attcttggca
      961 gtgtgacgac tgaaaagcaa ggacttccag cagatgaagc agagaaatac ctagcaaaag
     1021 gttattctcg gaatgaccgt gtaggacaaa gttatttgga aaaacaatat gaagatgtcc
     1081 tgcaaggaac caaaacccaa tatgaagttt cccttgataa tgaaggaaat gtttcgaatc
     1141 aaaaagaaat cttctctggg gaaaaaggtt ctaatttaat gttatctatg aatgcagaat
     1201 tccaatcaaa agtagaagaa attttgaaaa ggaattatca aacattgatc aacaacggca
     1261 aagcacagta ttcgcctggt gcttatgcag tggctatgaa tcctcagaca ggagaagtgc
     1321 tagcaatgac tggtttcagt catgaacaag gttccaaaga aatcaccgag aatgctttag
     1381 gcacgattac tagcgccttt gatccagggt ctgtagtcaa ggctggtact ttgacagcag
     1441 gctgggcaag taacgcgatc agcggaaatc aagttttgat tgatgagccg attcgtctgc
     1501 aaggtgcttc agaaaaaagc tctgtcttta atcgaagcgg acaagttgct cttgatgcag
     1561 tcaaagcatc ggaattgtct tctaatacgt atatgatcaa agtggcgtta aagatgttag
     1621 gactagacta tacacctgga atgggactac ctagtttaga tgaagaagct aaagcttatc
     1681 agcaattgag agactctttc aaagagtttg gtcttggtac aacgactggg atcgatttgc
     1741 cgaatgaatc tcctgggatc tcacgttctg ttgattatat gaagaagttc aatgcggata
     1801 atggcaaaga atggtatgct ccaggtaact ttaccgattt ggcatttgga cagtttgaca
     1861 catatacacc aatccaatta gttcaatatg cttcaacagt cgctaatggc gggaaacgag
     1921 tacagccgca cctagtcaaa gcgatctatg gcaatgacga aaatggaaac ttgggtgaag
     1981 tgaagaaaga aattggcaca acagtagaaa atacggtcaa tatttctgca gaaaatatgg
     2041 cgatcctccg tgaaggattc caccaagttg tccatggtac cgatccttac acaacagcca
     2101 aaccattagc cagtgcgaaa atggaccttt ccgcaaaaac agggactgca gaaacggttg
     2161 cggaaggtca tccagatatt gcgacagtca atagtaatat cgtggcatac ggaccaacag
     2221 acaatccgga aattgccatc agtgttgtct tgccgaactt attagacgaa caagaccaca
     2281 taaacttgac gattgctaaa gaaataatgg acgcgtatta tgatatgttc atggcaaaat
     2341 aaaaaagagg tcgtgaaaaa gccgcttagt ttcgagtatg aagaaaaatt ttgtgaaaat
     2401 agcttctcac atttttgctt aaatttgact tcataccgag aaactggctt ttgaacaccg
     2461 tttactcggc aaaaaaggtt gtgacaaaaa tt
//
LOCUS       DAYYME010000226         2456 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000226 DAYYME010000000
VERSION     DAYYME010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.231"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..1136)
                     /locus_tag="ACHBFW_002840"
     CDS             complement(93..1136)
                     /locus_tag="ACHBFW_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFM9319084.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1513..>2456)
                     /locus_tag="ACHBFW_002841"
     CDS             complement(1513..>2456)
                     /locus_tag="ACHBFW_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFM9319085.1"
                     /translation="PIPICGLILGLASLGNLFKDYHHVALGNLTGGISMILMIFIFVK
                     LFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWLCAVIFQVI
                     LVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFFWISLICYA
                     FLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLVVALFALSQ
                     VLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYSSVLDFLKV
                     AECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT          878 a          460 c          473 g          645 t
ORIGIN      
        1 gttgttgtta gaaggatatc tgcaaaatta aaaacagttg tgattcggac attgttccat
       61 gcgagaatca caactgtttt ttcatgttca ttctaaactt tgatcagcca aataaatagt
      121 aaagccatca ctgtttggag aagcccgaca gctaatccat aagataagaa gcgtgtacct
      181 tctttcatga attttgcaaa gtctaaacgc agaccaattg ccgctaaagc agtgatctca
      241 aaccagctgc tgataaaatg tgccgattgg tcaataacta caggtaaaga aaacaagctg
      301 ttgaccaaac aaaagatcac aaaccctaat acataccaag gaagcggtaa ttttcgtttt
      361 ttctgcgtat cagttggcgt ttcaaacaga gctggttgtt tcttgtgcat gaatttttca
      421 aacagataga caacaacaac gagcatcata atacgtagaa tcttaaatag catggcgaat
      481 tcgacaacag aattgtcaag taagcttgcc ccagcgacta cttgaccaac agattggagt
      541 gtaccaccca aaagtgcact tttttcaatg agatttccac caaacaactg taatccaaga
      601 aaagggagag tcaacatcat cacagtaccc agtagattga ctagtgtgat gatctgtcct
      661 ttttcttcct ctttcgcatg gatggaaggg gcgattgaag caatagcaga agaaccacaa
      721 acagcgtttc ctccagccat catcaaagaa gaagtatcgc tgaattgcag ttttttacca
      781 atcaagtaag caccgatgat agtgactgac atttgcagta acacaaaaac aattcctttt
      841 acaccaattt gagcaatcgt ttgagctgtg acagttgcac ctagtaaaaa aacagagaat
      901 tctaatagtt tgctttcagc aaattttgtg ccgattgcta gaacaggctg tttaaaaaaa
      961 gtatttccta ataaaatacc caataaaata gcaatagtcg ctccacctaa ctgtggcaga
     1021 taaatagcaa gaagcctgct gaaggcagcg atggcaaaag aaagcagtaa accgggtaag
     1081 atttcgcgga ccgctgaatc ctttacagat tttattggat gtgacaatga tttcatttga
     1141 tcggttcctt tcgtcaataa atactaaaag catactcgaa catagtagca ctaaaccagt
     1201 attttgacaa aagaacaaga ggtcgtgaca aaagtcttgt acgacctctt gtttcgaata
     1261 aacggtgttc cagaagtagc ttctacggaa ataagccgaa atttcacaaa aatttgagaa
     1321 gcaattttcg taaattcctt cttatttctt gaagctgacc acttctttta cgacctcttg
     1381 gtaaacggtg ttcaaaagct tgcatctgcg gtaataagcc caaccagtac aaaaacatga
     1441 acagctgttt ttgtttggcc gttcttatta cttgatgcag aacagctttt tcacaaccta
     1501 ttgaatcgat tattaagcga tttttttaga aacaaatttt ccatgtttgc tatgttcttt
     1561 aattaagaaa cgtacgtaat gagcaagaac gtagaagaca actacacttg caaacagaca
     1621 ttccgcaact tttaagaaat ccaatacgct actatagata cctaaactgt tgaagtaatg
     1681 aacagtagta aatatagctg ttgcagagat aacaagcggg aatgtaaagg ctgcatagct
     1741 tggataaaaa cgaagcttga acaaacgtgg aagcatagac agcacaacga aatacaatac
     1801 ttgagataaa gcgaacaatg ctacgacaag ataaaaatta ggttcagcaa agtttttaag
     1861 ataacctgtt aggcaaagag aaccaggtgc tgcaacgatc gtaatcagcg gacgtgtcgg
     1921 atgagccata tttcttacga aaaagacacg tttgatgatg atcggcaata agaacgcata
     1981 gcagatcaat gaaatccaaa agaaaatttt tccaataagt gggaagaagt tcccggcagt
     2041 tacagtgatg accccaaatc caacgtaaat gataaaccag cttggataaa ttgcttcaat
     2101 tgaaagatca gctttgataa catggtgata attgaaataa aagacaagga tcacttggaa
     2161 gataacagcg cacagccaaa tgaatttgac aattggtgca atactttcat atgaaacaaa
     2221 ataagtacaa ataaccatga gtgacatcgt aaaagttggt gaaacggagg cgatgattgg
     2281 atcatgcaac gtttgtttcg tgtgttcgaa taagataaac aatttaacaa aaatgaatat
     2341 cataagaatc atagagatac cgccagttag attgcctaaa gcaacatggt gatagtcttt
     2401 aaacagattt ccaagggaag ctaacccaag gatcaacccg cagatcggga tcggaa
//
LOCUS       DAYYME010000227         2449 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000227 DAYYME010000000
VERSION     DAYYME010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.232"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            668..862
                     /locus_tag="ACHBFW_002842"
     CDS             668..862
                     /locus_tag="ACHBFW_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319086.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            1053..1302
                     /locus_tag="ACHBFW_002843"
                     /pseudo
     CDS             1053..1302
                     /locus_tag="ACHBFW_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1546..2376)
                     /locus_tag="ACHBFW_002844"
     CDS             complement(1546..2376)
                     /locus_tag="ACHBFW_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFM9319087.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVDGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
BASE COUNT          727 a          437 c          484 g          801 t
ORIGIN      
        1 cctaaccatc ttattagctt ttatagggta tatgttggtt gggctgctaa aatcatacag
       61 taatgagttg ctgaattttt caacatttat taatgataca ttagttccaa gtttgttttt
      121 cattgtattt tttgctgttg gttattttat aataaagata tgatgactaa tttagctcaa
      181 ctattccagc gtcagctgaa gatattgcaa ttcccgattc cttaatcatt tatctggatg
      241 agacagaaag tgaagttgca acagatgtgc atgtagttga ggataaaaat gatagtttaa
      301 aaattaatag ttcagatact ttactaagag taagtcctat cttttcagct gttatttaga
      361 tgcaagtagc aactaagtta ggtagtactc ctgcaatgga taggtatttg tgtgttcgat
      421 atataagtgg tcgaagtatc aaggttatgt atactggatg ggagatagaa gagttaaaaa
      481 ttggaggtca ccgccacaaa tggaatttag atttagaggt accttagatt tggtatagtt
      541 attaaacaca agatggcctg gtttgtaggg aaagtcgaag agtatttttc cacttctgcg
      601 cccctttaat ttttctagta atgccgtgaa tttgtgatta attggaaata tgaagaaagg
      661 agagtctatg aaaacaacac tagttctatt ctataaaaag catccttatt ttacacttct
      721 gataaatata ttgttggcta gtgttatcgg gatttctgtt gaatatctaa taaataaaga
      781 tttcataggt tcttgttttt atacggcttt atttcttgga cttcttgaag cattttccat
      841 ttataaaaaa tcaaaaaaat aaaatagatt gccgtaagag atctttttca tttcattttt
      901 ttctgaaaac ccgttgtttc tgtcagtgaa gcagcgggtt tttagattta ttgtctttag
      961 acctaattga gcgcgccgag cgattgaaat acaaaaccac ttgctatgcg ggtggagcaa
     1021 taaagggaaa tagtttagcc cgtacaaagt agatgtgtaa ataccacatt gtattcacac
     1081 caaagtatag aagaaaaata atttataatc aatatcgagc caatattcag gagatcatca
     1141 agagattgtg caaatctaaa atataaattt gggtatcgac acttttgggt ggaaggatac
     1201 tatgtgacaa aagtaggaca aaatgaagcg accatcaaaa agtacattca agaacaagaa
     1261 aaacatgata gtgcgttaga taagctgagt gtacgagagt aagacgatcc ttttaagggc
     1321 taaggagtag tacataaacc ttttaagagg tgggcaaaac tggcaaaggc aatatggctt
     1381 gaacataaaa tatgaaagcc agcgtcttta ggcgctggcc ggtgatttga gcttatagct
     1441 ctagagaaaa ccaccctttt gaagggtggt tatgattaat gactttagtc agttttgctc
     1501 acaaagcgtg tggaacaaaa aacgggtgtt tctacgggta gatatttatt tcttgaatgg
     1561 aggtgtccca tacatttttg gtgttactgg ttctaatttt ggtgcagcgc cttcgatctt
     1621 aggttctttc aaccattgat attcgccttc gccatccagt gctttgccac tagcccaaga
     1681 accttcacta ctttggtctc cctctgagaa gttatagaag tcataagcaa tgtcttttcg
     1741 ttctttgtca cgcgggaaag tacttgggac taagattgaa ccttcttttt cttctaattc
     1801 tttcactgca cattcccata gattttgatg atagctatct cttgcaagga gaacagacag
     1861 catatctttt acgccacgat catcgatcat ttcgtaaata cgtgctactt gaagtcttcc
     1921 ttgtgattcg gcatttaaat tagctctaaa gtctgctaac aagttgccac tcgcaatgat
     1981 ataggcaccg ctccatggat tgcccacgct gtcagcagga cgaggtccta agccatctac
     2041 gatcgcgtgc tgtggattca ttccagagat aatggcaccg attgttggat ctttttgcat
     2101 cgctgcttct tgatcttcaa caggcgcacc ctctaataaa cgagcaacca ttgtcgccaa
     2161 catttccacg tggccgattt cttctgttgc aatgtccatc agcatatctt tatatttttc
     2221 ttctcctcgg cagctccagc cttggaacag gtactgcata gctacactca tctcgccata
     2281 ttggccgccg atcagctcct gcagatattt cgcgattaat ggattcggac gatctggttt
     2341 tgcttcaaac tgcaattctt tctggtgtct aaacatcaaa attcctccta atctattttt
     2401 aatacattct cattctgcta cgaaagatta aaaaattcaa agaaagcgc
//
LOCUS       DAYYME010000228         2399 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000228 DAYYME010000000
VERSION     DAYYME010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.233"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACHBFW_002845"
     CDS             316..570
                     /locus_tag="ACHBFW_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM9319088.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACHBFW_002846"
     CDS             571..888
                     /locus_tag="ACHBFW_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM9319089.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACHBFW_002847"
     CDS             1447..2382
                     /locus_tag="ACHBFW_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319090.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          865 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagagaaaa
//
LOCUS       DAYYME010000229         2346 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000229 DAYYME010000000
VERSION     DAYYME010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.234"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..708)
                     /locus_tag="ACHBFW_002848"
                     /pseudo
     CDS             complement(101..708)
                     /locus_tag="ACHBFW_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1231..1905
                     /locus_tag="ACHBFW_002849"
                     /pseudo
     CDS             1231..1905
                     /locus_tag="ACHBFW_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            1898..2059
                     /locus_tag="ACHBFW_002850"
     CDS             1898..2059
                     /locus_tag="ACHBFW_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319091.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          841 a          360 c          389 g          756 t
ORIGIN      
        1 tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataac
       61 catcagtaaa attacttgtc atactattga taaggagtat ttaattgaag agaatgatta
      121 ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat
      181 ctttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa
      241 ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc
      301 tataaaatct tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc
      361 ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat
      421 agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt
      481 ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg
      541 ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat
      601 agaagcagtt ttgttttgcc atccctcttt ttggtttagt gtgctagtaa tataagcacc
      661 ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc
      721 ctttactatc tatgacaagg acaacagttt taacatacag acattcatta aatttggata
      781 gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagacag tcaacttaat
      841 taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt
      901 ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat cttaaaagaa
      961 actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat
     1021 gaaaacaacc ctagaaatat ataactagtc caatgaaaaa aaagcgaatg ccgtagaaaa
     1081 caaggattct gcgtacattc atatctcatt ttgcgagcat atctgctatt actgtgacta
     1141 aatgttaata tggttaaaat gaccgttata ttttttatat aaatatcgta taattagtat
     1201 gaatatagaa gaaggaaaga aggtgcaatc ttggaaccta aagacaagta tttagattac
     1261 gctagggtca ataaagataa atttattgaa acaatcatca agggaaaaat ccctgatgat
     1321 gaaaaagatg cggtattcat ggctggaagc ccaggagcag gtaaaacaga ggttgcacta
     1381 ggtttagctt aaaattatga caaccatgtt gtgattgatg cggactattt tagagcacaa
     1441 tttccagaat acaatggaaa gaactcaagt gtttttcaga aagcttcttc ttggttagtt
     1501 gaacaatcat taaaatatgt actggaacat ggctattctt tcatactaga ggatactttt
     1561 gctatcctta gtgcagagaa aaatattatt cgagccctta aaaataactt tcgcgttacg
     1621 attttctatg tttaccaaga tcccaaagtt gcctgggatt tcactcgaaa acgtgaacta
     1681 gctgaaggac gtcatgtgcc aaaagaaatg tttatcaatg cgttttttaa gtccagagag
     1741 aatattgaaa aagtcaaaaa tagacatcct gaagtagtcc ttcacataat gattaaggat
     1801 tatcaaaacg acatttctga agtgcattat gcagcagata atattcaatt agtactacca
     1861 atacaatata cttcaaaaga gttggaggag gaattacatg actgagaaag aacaagtaca
     1921 acaaatcgtg aagaagtata ataaaagtat cgctgattta tcagaaaacg cctcagcgaa
     1981 agaatttaaa acagtaatga attacgtagc agatgaagca aatcgtaagc agcgaaaact
     2041 agttgggttg gataaataaa ttttttaaac catatccttg taaagttgag ttttttattt
     2101 taatgaatta agactattaa atagataatt attgtaactt aaaaaacaca gctaaaaatt
     2161 agttgtgttt tttattaggt ttacttttct ggtataaaag gaactagcta ctttttcaaa
     2221 atttctaaat cattcaatat gtagcgctta ctaaattatt cgccataaaa ttttataatt
     2281 ggatcactca ttgttttacc taagtcatat ttaagtaata accggtgttt ttacataaga
     2341 agactt
//
LOCUS       DAYYME010000230         2327 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000230 DAYYME010000000
VERSION     DAYYME010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.235"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            197..2059
                     /locus_tag="ACHBFW_002851"
     CDS             197..2059
                     /locus_tag="ACHBFW_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9319092.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2059..>2327
                     /locus_tag="ACHBFW_002852"
     CDS             2059..>2327
                     /locus_tag="ACHBFW_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HFM9319093.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIG"
BASE COUNT          834 a          347 c          457 g          689 t
ORIGIN      
        1 aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt ttaacagtac
       61 atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct ataagttgca
      121 cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta tattagcaat
      181 gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt tgatttactt
      241 ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa atgttagtgt
      301 tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc ggaagcaagt
      361 cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg atcgaatcaa
      421 attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc aaagtctccg
      481 aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg gaatgaaaat
      541 ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg tgctggaaaa
      601 taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt tttggatcat
      661 aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag agttcacgga
      721 ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg aatactttga
      781 aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa tcattcgaat
      841 gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat tactaaatat
      901 tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga ttggtcgtta
      961 tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg tcatgcagca
     1021 ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata tctatagtga
     1081 tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg tgatttataa
     1141 agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga cgaatctgta
     1201 tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat ataatccttt
     1261 taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc aagcagttcg
     1321 acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat atattgcctt
     1381 acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag ccgtctacat
     1441 ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg aagaattaaa
     1501 taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga ttgctaccaa
     1561 agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc ctttcatcaa
     1621 agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa taaaaaaaat
     1681 acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa cgtccatcaa
     1741 taaagatact ttagaagact ttagcaagga attgattttg aaagcttata cgatttatga
     1801 acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag cttttttgct
     1861 tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg cagaaggtcg
     1921 tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg aagcagtttt
     1981 tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct catatataaa
     2041 agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat cgaggaaagt
     2101 caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt agtagaacat
     2161 gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa tgagatgctg
     2221 aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt caaagaagta
     2281 tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcgg
//
LOCUS       DAYYME010000231         2315 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000231 DAYYME010000000
VERSION     DAYYME010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.236"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..2229
                     /gene="acm"
                     /locus_tag="ACHBFW_002853"
     CDS             64..2229
                     /gene="acm"
                     /locus_tag="ACHBFW_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFM9319094.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
BASE COUNT          916 a          354 c          443 g          602 t
ORIGIN      
        1 aaggattttt gagagatgat atagtaggtt ttatttttag ttaaaatagg ggagatagaa
       61 aaaatgaaaa agtgtagaaa tattatttta tcaatgttat ttattattac taatataaca
      121 tcactgattc ctgttcatgt ctatgcggat gcaggcagag atatcagcag taatgtcact
      181 tcgttgaccg tggacccaac gaatattaca gatggaggaa acataaaagt aaaattctcg
      241 tttgacgaaa aaaaacaaaa tatccaacca ggtgattatc tttggatcaa ttggccaagt
      301 gaaggaaata ttcgcggtga aggattccaa aaagaaatcc cattaatgat tgaaaataaa
      361 aatgttggaa ctttaacggt cagaaaagat tctgcgcagg ttgtgtttaa tgaaaatatt
      421 aaaaacctag actcagtaga aggttggggg caatttgaga tacaggccag aaacgtaacc
      481 gataccggtg aagaaaatac gggaccattc accgtgacga gcggtgataa aacagctact
      541 gtaaatgtga caaagccagc ttctggttct tcttctagcg ttttttatta taaaacagga
      601 gacatgctac ctgaagatac taagcacatt cgatggtttt tgaatattaa caataacggc
      661 acttatgttg aacaaccagt taaaatcagt gatgaaatcc aaagtggaca aagattagat
      721 ccaagtacgt ttgaaattaa tcaaatacac ttaggtgaac agaaggttta tagaggagaa
      781 gagggaatcc aacaattttt acaagatttc cccagcgcta catttaattt ttcagtaact
      841 gataattaca ttgaaataac tataccaaaa aactttgtca atcttaggaa aataatggtg
      901 agttataaga cgatcataga aaacccagaa caaataaatt ttgagaatca ttcggaagct
      961 tggtttaaag aatttaataa acctgctgtt gatggagaaa gtttcaatca tacagttaaa
     1021 aatatttcag cttctggtgg agtcaacggt acagttagag gcgaattgaa gattttcaaa
     1081 tatatcaatg acacagagat tggtatccca aatgtgactt tcgaattaag acgagcagat
     1141 gaacagccta ttcaaggaca gtctagtatc ctgttgactt cgaatgaaca gggagaagct
     1201 agtatcaaag gcctgcaagt aggagactat gttgtaaaag aaaaagaagc acccaattgg
     1261 attgattttg atccactaag ttcaaatgaa ctcaaattct ccataaacga aaatgataca
     1321 gagggagtca gtttacctat ttataacaag aaaaaggtta cgaacatcac agcaactaaa
     1381 atttggaacg gtggaaccac tccaagaccg agtatttatt ttaaattatt cagagcaagt
     1441 acaaacaatt gggaaccagt tcctgatgca gaaacaaaac gattagacaa tggaataact
     1501 tcagtgactt gggaagatat ccaacagtat gatgatagtg gaaacgaata tacgtttaaa
     1561 gtgcaagaag tagatcaaaa cggaaatgat tatgtccctt caggttatca aaaaattgaa
     1621 aacgggctag tcgttacaaa tgagaataaa gaagtcattt cagtctcagg acaaaaaaca
     1681 tgggaagaca atgagaacca agacggcaaa cgaccgacga cgatcacagt aaatctatta
     1741 gccgacggtc aaccgatcca acacaaagaa gtaagtgaaa aagatgattg gcgctatcgt
     1801 ttcacaaatc tgccaaaata cagagacggg caagaaatca tctatactgt aacggaagac
     1861 aacgtgcccg aatacagtac gacgattgac gggtataaca tcaaaaacag ttatacaccg
     1921 gaaaaaacaa atatatctat accaggtagc catataacac cgtggaaaaa ttttccagag
     1981 aagacagaga gagagaaaat tagaaatcta ttctctcaaa aattgcaaat atttgctcat
     2041 acaggagaat ccagcgaaca aagaagcaat tatattagta agagagctgt acctaaacag
     2101 ataacaaata aatcaaaaag cattcttcct aaaacaagta gtaaaaaatc tagttttgct
     2161 gtaatcattg gtttattgat agtaactatc tgtgctggga tattcttaca aaaatataaa
     2221 aaagtttagt tagaatataa tattttttgt taaatatgac tgaaatcaaa agaataaaat
     2281 tccgatttat aatcgaaaaa agaagcattt ttata
//
LOCUS       DAYYME010000232         2292 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000232 DAYYME010000000
VERSION     DAYYME010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.237"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1285
                     /locus_tag="ACHBFW_002854"
     CDS             <1..1285
                     /locus_tag="ACHBFW_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319095.1"
                     /translation="TVDIYYRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSI
                     SLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFW
                     YPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFI
                     LKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIIT
                     LVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTT
                     SDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTV
                     PVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILT
                     YCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1272..>2292
                     /locus_tag="ACHBFW_002855"
     CDS             1272..>2292
                     /locus_tag="ACHBFW_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFM9319096.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPW"
BASE COUNT          764 a          354 c          396 g          778 t
ORIGIN      
        1 aacagttgat atctattatc gatccgcaat tgcagaagca atcgctctta cgattatccc
       61 agctgtttta tattatgctt atggattgat attcaataga gaaaaaaaag tcatcgcttt
      121 atctgtcagt ataagtctac ttgtgtatac tcatgtctta actacactat tgtgtagtgc
      181 attgattgct tgtatgatta tagttcgttt gtttttaagc aagcaaataa aaaacgatat
      241 aaaatttgtg gtcgtgaatt tagtaaaggc tgtttttttg acttgtgcat taactagcta
      301 tttttggtat cctatgttgc agcagatgaa ttatcaagaa atacacagtc ccttcaaaac
      361 agatttgcag ctagaagcac tttctatttc agatagtatc attcaagcgt taaataacga
      421 tattacaaca tacactatgg ggttattagg attggttgcg ttgatttttc ctttattcat
      481 actgaaagaa atggagaaaa aagaaaaaat catctatgtt ggcgtagttt ctacatgggt
      541 atgcgtaact aatttatttc catggttttt gtttcaaaaa acaccgataa gcttactcca
      601 gtttccttgg cgtatcttag gtatccaaat ctttttttct tcaatgatca taacgcttgt
      661 ttttatgaaa aagactatga ataaaaaata ttcatggtta atcattggcg ctactgtggt
      721 gtttctactc gtcacgtcta tagcagcaaa agaaaattac gcaaaagtta tccaatcaaa
      781 acacggtcat attgttatca atgaagaaac actcccacgt tatacaacat cagatatggg
      841 aggattatac gattacgccc caacagaagc gataaaagaa aggcctcact tagaaaaaca
      901 tgaagtaaaa gtaggagaat cgtgggaaaa agggaagtat aaagctgcag ataataacat
      961 tacttacaca gtggcaagca acaaaaagca aaaagtgaca gtaccagtat atgcttattt
     1021 aggtacacag gtaaaagtaa acggtaaagt cgtaaatgac tcttataaaa aacatggctt
     1081 agtgaatgtg gatgtaaatg ctggagaaaa taagatagaa attacaacaa aatatactcc
     1141 aactgctctc atggctttat ggatcagtat tctgacttat tgtttagtgg tgtattcaag
     1201 ttttaaacga tttgcaccta ataagttgaa agattctcga tatgtgacta aaaaggagat
     1261 aaacaagaag aatgaaaata aataaaaaag tagtaatatt gagtggatta attatagtta
     1321 gtagtatata ttctttcatc tttttcaaaa atacacagag tatttatacc actggagatc
     1381 tttcttttca cttatctcga atcaaggggt tatcgactat ttttaccagt ccgattaatt
     1441 atgagacatt caattatact ggatatggtg taaattattt ttatcccttt ctgacttttt
     1501 ttccagctgt aatgttatat tggatgacaa aaaatttgat tgtaagctat attatttacg
     1561 tttggttatt gaacttgtgt acaacactcg tagcttatca ttatggcgag cggtttttaa
     1621 aacaaaaaaa agcggcattt ttatttagtt gtctctatat tttctctgct tatcggacag
     1681 tagacatcta ctatcgttcc gcaattgccg aagctatcgc aataacactt gttatccctg
     1741 tattatttta tgcttatcaa atcatctctg gaaaagaaga aaagtatccg tctgtaaagc
     1801 tagcattgag tatgagtttg ctagtgtaca gccatgtact atcaacatta atgagtacag
     1861 cattaattat catttttatt tttattcgtt tggtttctaa aggtttcaag aatgcagatt
     1921 ttattgctat atttaaaaaa ttatttagtg cggcaggaat gacattggtt ctgacgagtg
     1981 ctttttggta tccgatgttt gaacaaatgc tctaccaaaa aatcaacaaa cctagcgtga
     2041 caaatttata tgctcatgca tctaatgtgt tcgatagtct gactgaagct atgaacaacg
     2101 acttaactac ttactcaatg ggactagttg gtctgttatc tttatgtatt cccctgattt
     2161 tatttaaaaa attaacaaac atagaaaaaa agatttatta tggtacgtgt ttgacttggc
     2221 tagctacaac tagtttggtg ccatggtatc ttctgcaaaa tacgccagcc aaattgttac
     2281 aatttccttg gc
//
LOCUS       DAYYME010000233         2238 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000233 DAYYME010000000
VERSION     DAYYME010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.238"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..1685
                     /locus_tag="ACHBFW_002856"
     CDS             72..1685
                     /locus_tag="ACHBFW_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9319097.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          697 a          399 c          444 g          698 t
ORIGIN      
        1 ttttccggca caattatttg ctgtttttta ccataacata gtacgatcaa cgtagaaaag
       61 gagatgtttt tatgaaagtt gttgtcattg gcggtgtagc tggcggtcct tcatttgcca
      121 ctcgtttccg tcgattgaat gaagcgcacg aaatcattat ctatgaacgc ggagaaaata
      181 tttcttatgc aagttgtgct ttgccttatt atttaggagg tgtgatcact gaccgtgact
      241 cgctgatcga acgtacacca gaaatattga aaacaaaaaa caacatcgat gtctttacta
      301 aacacgaagt aacagcaatc gatccttcta ctaagcgatt aacagttaaa gacctatcca
      361 caaatgaaga aataaaaaca gattacgata agttgatcat ctcttctggt gctagaccag
      421 attatccgga tattcccgga gtttttgaag cagaaaacgg ctttgtcctc cgtagtgtga
      481 cggatgcgga tcgaatcaaa tcgttccttg aagaaaaaaa tccacaacat gtcgtcattc
      541 ttggtgcagg tgttatgggt ctggaattag ctgagaattt caagcatcgc ggcttagacg
      601 tgactttaat cgatcaattg ccacaagtcg ctttccctta tgatccagaa attgctaatt
      661 tagtttatga caaattgctg aaagaaggat tagccgttca tttagaaaca agagttactg
      721 agatccgtga taaaggtcga gaaatagtat tatcagatgg ctccgtcctt tctgctgata
      781 tgctaatttt tgctgttggt gtttctccga agaatgaagt ggtgaaagca accggcatac
      841 aattatcaga tacaggtcag atcattgttg atgaccagtt acaaaccaat ctgccggaca
      901 tctatgcgat tggcgatatt atcgaaacga ctagtgtagt gactggtcag ccgatccaaa
      961 gtatgctttc cagtgcggcc aatcgtcaag gacatatgct ggcagatatt ttaaatggta
     1021 cgcctatgcg ctatcgcgga tatattggtg caggtgtcgc aaaaatcttt gatcatacag
     1081 caagttatgc gggaatgaca gaacatgaat taaaagcatc aggcataaca aattataaaa
     1141 ctgtttttat cactcccttt gaccatgcct atttctatcc agaagctaca agattaaatc
     1201 taaagctgat ttttgatgca gatagcggtc gtattttagg tggacaagca tttggagaaa
     1261 aaggtgtcga taaacggatg ggagaacttt ctgtagcaat caccggaaac ttgacagtct
     1321 ttgatttgcc tgatttggag ttgccttact ctccaccgta ttctactacc cgtgacccgt
     1381 tgaatatagc tggttatgtc gcaatcaatc aaatgacgaa tatcgtagaa acgatcaaag
     1441 caagtgatat acccgaaaac gatttgaaag aagcgttctt tttagatata cgtgaaccta
     1501 ataaagcacc ttctggcagt atctcggcaa caaaaaatat tccaatgaat gagttacgag
     1561 atcgaatcaa tgaaatccca aaagataaaa aagtttacat tactttcaga agaggattga
     1621 atacttatac ttctgcccga atcttggcag gtttgggtat caaagcgatt ttgattgaag
     1681 aataaaaaaa cgaccgcggt gggtagccgc ggtctaggag ttatttttac ttgaggagta
     1741 aaaatgaaaa agtatatagg gttgttggtt ggtatgtata tagaatagaa gataaatgtg
     1801 aagaaattgt gcattatttc ttcacattta ttaggttata gtttaagaaa gtttaaacat
     1861 tgcgcaattt aattatttta gctgtttctt ttcataaaaa aacatgttgt gactctgaga
     1921 caaatcatag tccatagtga tgtctctaga gtaactacgc ctaatattac gaggcatcat
     1981 attaatctat cagcactagt acctaaacta gcatcccttt cgaatatttt ttcttatata
     2041 ataaagtttg ctttcactcc tctaatctca ccaaatcgac tggcttgcgt tgtgtgatac
     2101 taggaactga ttggtttaga acgacttttc tattgtaatc atatttttcg ccatcctcgt
     2161 tgtcttctaa tccttcaatg aatgatccct aatgtgtgta ttaaataatt taattttttt
     2221 gttcctattt atttaaca
//
LOCUS       DAYYME010000234         2223 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000234 DAYYME010000000
VERSION     DAYYME010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.239"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..183
                     /locus_tag="ACHBFW_002857"
     CDS             <1..183
                     /locus_tag="ACHBFW_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319098.1"
                     /translation="AKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSDKKIAYPLLLL
                     LFSFYQVQKLFCFVIC"
     gene            complement(160..1770)
                     /locus_tag="ACHBFW_002858"
     CDS             complement(160..1770)
                     /locus_tag="ACHBFW_002858"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFM9319099.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(1992..>2223)
                     /locus_tag="ACHBFW_002859"
     CDS             complement(1992..>2223)
                     /locus_tag="ACHBFW_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319100.1"
                     /translation="CFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHLKFFSN
                     DFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
BASE COUNT          665 a          471 c          358 g          729 t
ORIGIN      
        1 gcaaaaaata aaatatccaa tagcgcttta atcgaaaaaa gtcttttgct ttctatcttt
       61 tttcctacaa ataataacaa atcagaaaaa gagaaaagtg acaagaaaat tgcttatccc
      121 cttctcttgc tacttttctc tttctatcaa gttcaaaaat tattttgttt cgtgatctgc
      181 taatgatgct ccgacaaatg ctttgattaa tttttgcggg cgatttggac gagaaatcaa
      241 ttctgggtgg aattggcatc ccacaaagaa ttttttctct ggcagctcta cgatttctac
      301 taaacggtta tctggtgaga cacctgaaaa gactagccct tgttcttcaa acaattgacg
      361 atatttgtta ttgaactcat agcggtgacg gtgacgttct tgcacaacat cggctccatc
      421 atatgcttca gccgtcttcg ttcctttttt gattttacat gggtataatc caaggcgtaa
      481 agttccaccc aaattctcga tgttttcctg atcagccatt aaatcaataa tattgtttgc
      541 tgtatctgga ttcgtttcag ctgatgacgc atcttctaat ccaacgacgt tacgtgcaaa
      601 ttcgacacaa gccatctgca taccaagaca aatacctaaa aacggaacgt cattttcgcg
      661 agcataacga atcgcttcga ttttcccttc tactccgcga tcaccaaatc caccaggaac
      721 caagattcca tctgcgtctt tcaaacgttc ttctacattt tctctcgtta attcttgtgc
      781 tttgatccaa tcgatctcga tgtctgaatt ataggcgaaa ccagaatgtt tcaatgcttc
      841 aactactgag atatatgcat ctggtaactc gacatattta ccgacaagcg caatccgtac
      901 ttttttcttc aagttcaaga cacgttcttc taattctttc cattctgtca tgtctgcttc
      961 tggtgtatct agttttaaat ggtcgcaaac aatttgatcc attccttgtg cttgtaaaac
     1021 taatgggatg gagtaaagtg tatctacatc tggcgactcg ataactgctt ctggttcgac
     1081 atcacaaaat tgtgccaatt tgtttttcac gctttgagaa acaggttgct ctgtacgtac
     1141 caccaaaata ttcggttgga tacctaaacc acgtagctct tttacactgt gctgcgttgg
     1201 tttagttttc atttctccag cagctttcaa gtaaggaatc aatgttgtat ggatatacat
     1261 aacattatct gtaccgacat ctgctttcat ttgacgaagt gcttcaagga atggcaaaga
     1321 ttctatatcc cctactgttc cccctacttc tgtaatgatc acatctgaat cagtcatttt
     1381 agctgctcgc atgatttttt ctttgatctc atttgtaata tgaggaataa cttgaacagt
     1441 tgctcccaag tattcgccct tacgttcttt acgcaaaact tcagaataga tttttcctgt
     1501 cgtcacgttg gagtatttgt tcaaattgat gtcaataaaa cgttcgtagt ggcccagatc
     1561 caaatctgtt tcagcaccat catctgttac gaatacttct ccgtgctgat atgggctcat
     1621 cgtccccgga tccacgttga tgtatggatc aaatttttgg atagttactt ttaagccgcg
     1681 gtttttcaat aagcgtccta aagaagctgc aacaatccct tttccgattg atgaaacgac
     1741 accgccggtc acaaaaatat attttgtcat aagttcaaaa actccttttt aatttatgtg
     1801 caggaaaagt ctcagacaat aaacaaagct ccctattctt caataagaat agggagccga
     1861 aatttctgta aacttctgac cctctaaaag ggtgcccaag gtgtataata caagctgact
     1921 aagggaaagt caagtactca aatgaatact atccgcttac aattttttct ttttcgatat
     1981 cctttgagaa attatttctt aagtccttta tgagtgcaaa aaaaatttaa cccacataat
     2041 aagcttgata taaacatagc taacgttaaa cctaagcaaa tcagaaaatc gttactgaaa
     2101 aattttaagt gaaataatag ataaaaaaat aaaacaaaca ttatgataga taatatccca
     2161 cctaacaaag aactactttt tcctacttgc agcaacagtt tatatagctt atcgtagaaa
     2221 caa
//
LOCUS       DAYYME010000235         2141 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000235 DAYYME010000000
VERSION     DAYYME010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.240"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..718
                     /locus_tag="ACHBFW_002860"
     CDS             47..718
                     /locus_tag="ACHBFW_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319101.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            777..1217
                     /locus_tag="ACHBFW_002861"
     CDS             777..1217
                     /locus_tag="ACHBFW_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319102.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1229..1897
                     /locus_tag="ACHBFW_002862"
     CDS             1229..1897
                     /locus_tag="ACHBFW_002862"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM9319103.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1961..>2141
                     /locus_tag="ACHBFW_002863"
     CDS             1961..>2141
                     /locus_tag="ACHBFW_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFM9319104.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAR"
BASE COUNT          778 a          336 c          436 g          591 t
ORIGIN      
        1 tttcaaaata tgctatttta aaaatgatga aaaaaggggg agaaaaatga attttttagt
       61 tgttttaaaa gatactctga ttgagcgatt tacatttcta tatgttcaat tgcaggaaaa
      121 taaaacattg ctgcattttt caccagagtt ccatctagag gggctaacat taggagaaat
      181 ctatcaagca gaagggctgt cggctgtttt gcactttttt aaggtagaat tggaattacc
      241 agtaaaagat tatatcgagc tttctcttga atcttgggat cgagcagtgg ttgaactgtt
      301 tccagaaggt ttaatgatcg atacaaacga tgggaagatc catctgactg cagcagaact
      361 gcaaaaacaa atgcgttatc aaatagatga cgaaaattct ttttctattt ttagacgtca
      421 gcaaaagatt ctaaaaagct ttttaaaagt gatcagtaaa aaacggaatc tgatcaaaac
      481 gacacagctg ttgaaaaaat atccaggtct gcttcacacg tctattcaat ttcctcaatt
      541 gattacgatg atccatcgtt ttatacgtat gcaacagcta ccatctaaga agctgtttct
      601 tccattgacg gacacatttc gagtagtcaa cagagaagat aaaaagaaag tgattgtgat
      661 tgactttacc agaaatcgga atgtccttca ccaagttgca atggaaaagg ccaattaagg
      721 ctttttcttt ttggattttt ttgtcataat aaatattaga tgatggagga gataatatga
      781 tgcaaacatg gttagaaagg ctaatacgat ttcagcagaa ctatcacaaa cttatgcagg
      841 gaagatatgc gaaatttgat cagttgaatc gcggtctatt gatcgctgcg cttgtcttta
      901 gtttgctaag tcgttggttg ccctatagaa tcggacaatt gttgttcatc gtctttttcg
      961 gatggatgat atttcgtttc ctatcaaaaa aaatctaccc cagactaaat gagaatcaac
     1021 gttatttgaa atatcttgat agagtcaaag aaaaatatca tcagataaaa aacaaaaagc
     1081 aagtcaaaat ggatcgaaaa agttatactt tttttgagtg cccgaattgt catcaaaagc
     1141 agcgtgcacc taaaggaaaa ggacgaatac gtgtgacatg caaaacatgt ggtataaaat
     1201 tcgaaacgaa tgtttaaggg agaaaagaat gaaattagta ttattacgtc atggcgaaag
     1261 cgaagcaaat tttgaaaatt actggacagg atggctagat gtcgccttaa cagaaaaagg
     1321 aaaagaacaa gcagcagttg ccggtcaaaa aatgcttgaa gccggacttc aattcgatgc
     1381 cgtttatacc tctgttttga aacgtgcgat tcttaccgga caagtcgcac tagaagaaat
     1441 gggtcagtta tggcttccta taataaaaac atggcgtcta aatgaacggc attatgggga
     1501 tcttgtgggt aaaaacaaag ataagatgaa agaaatatac ggaaaagatc aagtaaaaac
     1561 atggcgcaga ggatatacgg aagttccgcc gttaaccgca gaaaatagat ttgaccgcag
     1621 atatgatcac ttggatccgc atttgatccc atacggagaa agtctggaaa tgacagtgaa
     1681 tcggatcatt cccttatggc aagatcatct agcaccaaaa ctaaaagatc ggcaagattt
     1741 actagttgtg ggtcatggta atagcatccg agcactgaca aaatatttag aagatgttcc
     1801 tgaagatcaa atggatacga tcgatatccc aaatgcacaa ccaatccagt atacattagc
     1861 agatgatttg acaattctgg acaaaaaaat actttaaaga acacctttat ctaaaagtga
     1921 gagatagaaa aaagaagcaa aggaatggaa caaaaggaac atgaaaaatt atatatggga
     1981 ctttgatggt acattgtttg atacgtatcc cgcaatggta gatggtgcat ggcaagcact
     2041 aaaagatttt ggtatcagta tggacaaaaa agaaatctac ttcaaaatga aaaagtattc
     2101 tactagttat ttgatcaatg aatcaaactt gaatgccaga g
//
LOCUS       DAYYME010000236         2138 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000236 DAYYME010000000
VERSION     DAYYME010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.241"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..510
                     /locus_tag="ACHBFW_002864"
     CDS             46..510
                     /locus_tag="ACHBFW_002864"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9319105.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            524..1273
                     /locus_tag="ACHBFW_002865"
     CDS             524..1273
                     /locus_tag="ACHBFW_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9319106.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            1273..2103
                     /locus_tag="ACHBFW_002866"
     CDS             1273..2103
                     /locus_tag="ACHBFW_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9319107.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
BASE COUNT          683 a          289 c          424 g          742 t
ORIGIN      
        1 gcaatttcta ttctataaat tttaaaaatt gaaggaggaa atagaatgat taaattagtg
       61 cgtgtagacc acagattact tcatggacaa gtaatatttt cttggactaa acagtatgat
      121 attaaccata ttattgtggc tgataacaaa gttccaggag atccaatgtc tgtgatggct
      181 ttgacaattg ctaaacctgc tgattgcaaa ttagacattg ttaaaattag tgatgttaaa
      241 gggattatgg aaacatcccc aaatgataat tacatgattt taattaaagg acctaaggaa
      301 gcttttgaat tagttcaaaa tttaccagaa attaaagaaa ttaattttgg cggtgtgtct
      361 aagaaaagcg agtccaaaca atatggaaag gcagtctttt taactcctga agaattagaa
      421 gccacaaaaa aaattcttga tcaagaagtg aaaatatttg tgcaacaagt cccatcttca
      481 agtattgaaa atgtagattt tagtaagtaa ggggggaaaa taaatgttat taaaagccat
      541 tttattaggt ttagttggtg tggtagctgt tattgactcc aggttaattg gtcgacaaaa
      601 tattggtaga ccactaattt tgagtacact tgccggactt gtgctaggtg acttgcatac
      661 agggattgtt cttggggcat ctttagaatt aatttctatg ggattcgtct caattggggc
      721 ggctggacct cctaatatgc aacttggtag tattattgcc actgcttttg caattttaac
      781 taatagtagt acagaaacag ccttaacaat agctatccca gtggctgtag ctggtgaatt
      841 tatgagtatt atcatgcgca tgataattgc tcaatttgga catgctgcag ataaagccat
      901 tgaaaataat aaatttagaa aagctcaaat tattcatatt tattggtcat ttatttttaa
      961 tgcttttgtt tactttattc caatcttttt aacagtatat tttggtgctg acgttgtggc
     1021 agatttagta gataaaattc cagaaatgat tacagacgcc ttgactgtct ctggaaatat
     1081 gctttccgca ttagggtttg caatgctatt aagcacaatg ttgagtaaaa aattatatcc
     1141 ttactttatt tttggattct ttattgttgc ctattcaggt ctttcactaa ttggcgtgac
     1201 aatttttgca gctctgattg cctttgtaat ggatcaagtt aaatatggaa aaagagaaga
     1261 ggcgcatggt taatggaaaa tagtacagaa aaaaaacgtt taatcacaaa aaaagatctt
     1321 aataagatgt gtcttcgttc atggacaatc atgtgttcgt ggaattatga aagacaaatg
     1381 catatgggat ttatgtatgg gatggcgcca attttaaata aagtttataa aaatgatgaa
     1441 gagaaaaaaa gagaagctta tactcgtcat atggcatttt ttaactgtac gcctcaaatg
     1501 acttcattta ttatgggact ttgtgcttct atggaagaac aaaatgctca agcagaagaa
     1561 ggaaacttta atactgaatc tatctccatg attaaaacca gtttaatggg tccttttgct
     1621 ggaattggtg attcattttt ccaaggtact ttaagaatta ttacatttgg gattggattg
     1681 tcttttgcta agcaaggtag tattttagga ccgattttag ctgttggcct ttttgctata
     1741 ccttctattt tagctgtttt ttacggcggt aaattaggat atgagtctgg caataaatac
     1801 cttactaaat tgtatcaaga aggtcttatg gatcgagtaa tgcaatttgc ttctatcatt
     1861 ggtttggctg tagtaggtgg aatggtggct agcatggtgc caattgtcac ccctttaaaa
     1921 ttcactacag ggggaacaga acttgtttta caagacttac ttgattccat tattcctgga
     1981 ttattaccac ttgtatgtac tttgggaata tataaattga ttaagaaaaa tgttagtaca
     2041 aatatgctct tattaggttg tattttggca gggtttgttt taagtataat aggtatctta
     2101 taatgattaa ggctctaatt tttgatatgg acggagtt
//
LOCUS       DAYYME010000237         2060 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000237 DAYYME010000000
VERSION     DAYYME010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.242"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..253
                     /locus_tag="ACHBFW_002867"
     CDS             <1..253
                     /locus_tag="ACHBFW_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9319108.1"
                     /translation="TEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNG
                     VTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            260..1873
                     /gene="guaA"
                     /locus_tag="ACHBFW_002868"
     CDS             260..1873
                     /gene="guaA"
                     /locus_tag="ACHBFW_002868"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM9319109.1"
                     /translation="MRRTERAAYRTKFYSNLSSPTRFDEEPKIIVLDYGSQYNQLITR
                     RIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVL
                     GICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVT
                     QAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTG
                     DWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVL
                     LRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDE
                     ATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLF
                     KDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANA
                     GLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKI
                     SVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT          632 a          431 c          429 g          568 t
ORIGIN      
        1 tacggaaggc tttcaagaaa cccttaaaac aatttcatct acgttacctc agtcgtttaa
       61 aaagaaattc acgatcttcc atcgctacca atcgggtatt tcaaatgcct ttaaggtgtc
      121 ccactcaaac ggcgtcactg aggggctgaa taacaagatc aagctgatta aacgaattgc
      181 gtttggttat cgcaactttt ataactttcg ggcacggatc tatttacaac aaggtctgat
      241 ctttgaaaac taaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta
      301 ttcaaatttg tcttcaccaa cacgatttga cgaagagcca aaaattatcg tacttgatta
      361 cggtagtcag tataaccaac tgatcactcg ccgtattcgt gagttcggtg ttttctcaga
      421 gttgatgagc caccgcatta ccgcagaaga agtaaaggca atgaacccta aaggaatcat
      481 cctttctggc ggaccaaaca gtgtttatga tgagaaggcc tttggtattg atgaaaagat
      541 ttttgatttg gggatcccag ttttaggaat ctgttacggt atgcagttga ttacttataa
      601 attaggcgga aaagttaaac cagccaaaaa ccgtgaatac ggaaaagcag aattggaaat
      661 cacttctgca gaagcggccc ttttccaaaa cactcctgaa aaacaaaccg tttggatgag
      721 tcatggtgac ctagttaccc aagcaccgac tgatttcgat gtcgttgcta ccagcaaaga
      781 ttgccccatc gcatcgatcc aaaatgcaga acgcaaaatg tacggaatcc aattccatgc
      841 agaagttcgc cattccgaat atggaaatga cttgttgcgc cattttacac tagatgtctg
      901 ccaatgtact ggtgactgga caatggataa cttcatcgac atggagatcg caaaaattcg
      961 tgaacaagtc ggtgacaaaa aagttttatt aggcctttca ggcggagtag attcctctgt
     1021 tgttggtgtt cttttacaaa aagcgatcgg cgatcaatta acatgtatct tcgttgatca
     1081 cgtattgttg cgtaaaggtg aagcagaaca agttatggaa agtcttggcg gaaaatttgg
     1141 cttgaacatt ataaaagtcg atgccaaaga acgtttctta tcaaaacttg ctggcgtgtc
     1201 tgatccagaa gaaaaacgta aaatcattgg aaatgaattt gtatatgtct tcgatgatga
     1261 agcaaccaaa cttgccggtg aagaaggcgt ttctttcctt gcacaaggaa ccctttatac
     1321 cgatgtgatc gaatcaggaa ctgaaactgc ccaaaccatc aaatcacatc acaatgtagg
     1381 tggattacct gaagacatgc aattcaagct gatcgagcca ttgaacacat tgttcaaaga
     1441 cgaagtacgt gaattaggta cccaattagg catgcctgac agcatcgttt ggcgccaacc
     1501 attccctgga ccaggcttag gtattcgtgt ccttggtgaa ttgacagaag aaaaattaga
     1561 aatcgtccgt gaatcagacg ctatcttgcg cgaagaaatc gccaatgccg gtttagatcg
     1621 cgacatctgg caatacttca ccgtcctacc aggcattcgc tctgttgggg ttatgggaga
     1681 tggccgtacg tatgactaca ctgtaggtat ccgtgctgtc acttctattg acggcatgac
     1741 cgctgacttt gcccgtatcc cttgggaagt cttgcaaaaa atcagtgtcc gtatcgtaaa
     1801 tgaagtcgca cacgttaacc gtatcgtgta tgatattacc agcaaaccac ctgcaacagt
     1861 ggagtgggaa taaagagcca acataatatg gatgaggaat acctataaat caacattatt
     1921 caattactaa agttcctgat tttcgtaaaa aataagatgg tttcctacca ttttccctac
     1981 caaagccctt gttagattaa ctactaacaa gggctttgct tttattcact acctttattt
     2041 tcctattgaa ttagcttttt
//
LOCUS       DAYYME010000238         2040 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000238 DAYYME010000000
VERSION     DAYYME010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.244"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..1102)
                     /locus_tag="ACHBFW_002869"
     CDS             complement(62..1102)
                     /locus_tag="ACHBFW_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM9319110.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          710 a          298 c          255 g          777 t
ORIGIN      
        1 ataaaaaatt tttaatttta atgttactcc atgaagcaac aatcactttg tcagtaaagt
       61 ttcactctaa ccagtctttc gtaatagtgc ttggggagct tgcataattc aaacgttttt
      121 cacttgatac tttagcagct attggttttt cgataaacca attttttaca tagacaaaaa
      181 tcaggttctg gatattttcg actttttcgg tttttaattt ttgataggct tttaacagtg
      241 tcgtgtataa ttcttgttct gcattgacat aataactatc gagttcttca aaaactatcg
      301 aaatatcact ttctatttca gcttttttct ttgcattatg aatagtctgg acggtttttt
      361 gagcttcact gtaacttgaa gaaaatgttt ttatcaaatt taatactcgt tcagggacaa
      421 acgtagcaat gctgtcgttt gtcataattg tctcaaaatt gtctaacaac aattgatctt
      481 gtaggcggtc tttttcagtg tctatattgt gtctattagt gtctaatata ttattattaa
      541 gttctggtat gattttcata ccctctgatt tatcaacgtt ttggcacggt atgattttca
      601 taccctcttg agccccaagg gattcagcgt ctgatttttc gtcgagggtg ggtatgattt
      661 tcataccctc tgatttatca acgttttccc tattctctct atcaaatttt tcgagtaagt
      721 agatatcatt ttctgagact tttaattctg ctagatatag tctattagga ttatttttcc
      781 ctaactgttt gttaaatccc atctgctttt gtaataataa accaaaggat tccaactctt
      841 ttttgattgc tatcgctttt tgtttttggc agtttaatac tctacatagt tcatttgtcg
      901 tataagtaaa ataaacatta ccttcttcat cgatttgccg tttgctgatt gcgatctcta
      961 atcttgcttt taaaagcata taggcaatct tagttgaatc tttcattttt ttgtattcgt
     1021 cagaaacaat aaagactttt ggaaattgat aaaataattc gttgtaaact ctatccgcct
     1081 taaaaaattt aaaatcagtc attgtagatc ctctactttc acgttgtttt atttatgtaa
     1141 aattacctac tcttttcgaa aatagcaaag ataaataaaa aatgcaaacg cattaaaact
     1201 gttttttaat taaatactac ctaaaatcag aaataaaatg aattatgtac tacctttaaa
     1261 gtcattaaga actactttaa ataaatatta tttataatta aatactacca gaaaaacgta
     1321 tgaataaaat ttagttttaa aatcaaagat aattaagtac taccagtgtc aaaacaaaaa
     1381 ataaattaag aactacctta tgcatataaa acatactacc attactagta atatatacta
     1441 ccttatttca gtagtatata ttatattatt cttataagac aaggctttag gctagaaaac
     1501 aatagattat atactactaa aaaatacaat aagtactacc aatgtcagat atatataata
     1561 attatgtact accattaaaa ttaacttatt taataagata ctactgtgtt aatctgaaaa
     1621 aaattacttt aaagtagtta catactacct gttaatttag atactaccat taatacttaa
     1681 attagatact acctatattt atattaagta ctaccgttct aagaataaga taataaaaaa
     1741 atactaccaa attgcaatta aatactactt aaaatgtaaa aaataagaac tacccaaatt
     1801 aggtagttct taataagaga atatcagata aatttgtaca agtcataaaa aaattaatct
     1861 taaattttta aatatatata cttgctaatt ataaactact ttatttaggt agtacataat
     1921 taacagataa aactacatgc ttgtagtttg tctagctaat gagttgcata tgatttatat
     1981 aaaggtgtgc agcataactt agttgaagtt aagagaatag aataaatatt ttttgttttg
//
LOCUS       DAYYME010000239         1956 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000239 DAYYME010000000
VERSION     DAYYME010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.245"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..426)
                     /locus_tag="ACHBFW_002870"
     CDS             complement(<1..426)
                     /locus_tag="ACHBFW_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM9319111.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(535..1566)
                     /locus_tag="ACHBFW_002871"
     CDS             complement(535..1566)
                     /locus_tag="ACHBFW_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9319112.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          603 a          323 c          339 g          691 t
ORIGIN      
        1 aggaatctgt tttgattttg ttccatttga aatttctctc actcgctttt ttgtctcatc
       61 atcaaataaa atgaaagggt cttctaaacg atcgataaat acttccattg ttgttgttgg
      121 tagatgataa gaagtcatga caggcactgc accaaggtaa gacacagaaa cagctagaag
      181 gtatgtgtca aaagcggaac tcttataaat aataactttg tctccggatt tcactccaag
      241 atgagccaat tgataagccc gtttcaacac actttcgcag cattttttgt aagttgtttc
      301 aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag ataatcgggg tctcggaaaa
      361 gttagctgca gcttcttgaa aattcgtaaa taagttcaaa ggggaatacg ttatggtctc
      421 catcattcac actccattat cttttgttaa gtaatataca aattcaagat aatcgtaaca
      481 taatccttta tataataaca tagtttattg tgaaaaaaag atcatacatt tgttttatat
      541 ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa aaatcaacca tcttttccca
      601 gatatcttga aaagaatggg atgagaggta tcgattacca cataaataat ttaccgcgtc
      661 agcatcatct aaatatagtt ctgcataata acgatatgtt tttctgcata gttctggcga
      721 tacgttttta tatactgtaa ttttgctcaa aatttggcga atagtccgag gagaaatttg
      781 ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc gtattttgtg ctgttaactt
      841 aaaatagttc tccagaaatg gcatcgcttc ttttggtatt ggtactttat tcagtctgtt
      901 ttcttttcta ttcagattaa gaatctgctt ggtcaaagat acatctttac gtttaagttc
      961 tactacttca tggatcttta atccgcaata cagaagaatg gcagtaattg caaggttcct
     1021 cggtctattc ttttcttcaa agctagcttc tctgcttgtt tgaaagcctt caatcgggat
     1081 aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata tatcgcggag ctgctttggt
     1141 tgtttccggt aaacttgtac ccaaaagaga aatctcttct tcaatcagtc ctttttgata
     1201 caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt gaaatcccgt aatagctatt
     1261 ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg aacgaacgag tatgtcgata
     1321 gattttagct aagaaattct gataatcttg tttttctaaa tggataatca gctgatcatc
     1381 cacttcttca gaaaatactt ttctcaaata atcagtaaac agatgaaaat cttttgcgta
     1441 ttcatagcga ctgcctaatg aaagggaaga acgattttct tctaaaaact gttgaaagac
     1501 ggaagggagc aagtgtgtac gttgctttaa cttttcttgc agttgttttc tagttttgct
     1561 gtccattaca aattccctcc aatagtgttc attttctata ttttaccgca tttcatcaaa
     1621 tgaagataga cagtttgtct tgatcttagg aagcaaattt ttaactaagt actagctaaa
     1681 agatatgaag taagaagata catttttagt tattttatta gtgcagtcat tcttttgatc
     1741 ttttaattga tttatcctct ttttagctat gtggttactt catgagcaat gaaatggatt
     1801 tggttcgttc taatggcagg aggctctttc ttttatttcc gaaaaattag aaaacatttt
     1861 ttcagaatcg attggctctc catttattca ataatggttt gtctaacgat attgttggga
     1921 taattcttaa agaagtatta gaaatagttg gtactt
//
LOCUS       DAYYME010000240         1937 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000240 DAYYME010000000
VERSION     DAYYME010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.246"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            552..884
                     /gene="traE"
                     /locus_tag="ACHBFW_002872"
     CDS             552..884
                     /gene="traE"
                     /locus_tag="ACHBFW_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFM9319113.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1503..1844
                     /locus_tag="ACHBFW_002873"
     CDS             1503..1844
                     /locus_tag="ACHBFW_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM9319114.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          672 a          262 c          414 g          589 t
ORIGIN      
        1 taaacaagct aaaaatcttg atattacttc ttttcaggca atacgaatat tattcgctta
       61 cattttttgt ataaatttaa ttgtctgcta tgcttgtgat gaattgattt tcgttttgca
      121 cttttggatg ataaatttgg acaattgaaa atacaaaaaa aagaaaggcg gtatgaactc
      181 ggaaggctgg taaccttcgt cagcgttcat acacggcatt aagatttggc ccttaatgcg
      241 ttaacctttc agtgttacgg tattacccgc aatggattta gtataacaga tattcaaaag
      301 tgataccact ttttttggct gtgttaaggt taaaaatacg tgatattacg atgaatctat
      361 gcctaagcat aatgttataa aaatgtccgt gtggctcttt gccgtatgac gaacgctgaa
      421 attagaggga acgcatgttt ggatgcgttc cctctttttt gtttgtcatg aacgaaaatc
      481 aattctctat ttttgaaaaa taacaagaaa aacttgttaa aaatatttaa agaaattggg
      541 tggtttattt tatgtcatat acaggaattt tgagtctcaa agatatttgt cactacggta
      601 aacggtgtac cgctacagag aaaatcacga agaaactatc aactggccaa agtaaaaccg
      661 ttgtgcagtg taagaaatat ataatccaga aagacaaagt ctcagaggaa atgatttatt
      721 acattgggaa acaaaagcaa ataattttaa aagatcctat ccccttaaaa gagctatatc
      781 caacaatcaa acatgtttat gaccaaaacg gtgtactgat tggtcgaaga aaaaatggag
      841 tcttgcgttg taccgcaaaa gggatggggc gtttgatcgg ctgaattgat cattctcata
      901 acttgtattt tatcgttttt cgcttgcttg aaaatattgt cgagacttct aaaggaactt
      961 agagtcacta gaaaaataag gagtagactt gatctatcaa caattccctt cggggagtga
     1021 cggggtaggt tttaacgatt ggtaacgtaa aaaattggtc aatcgagtaa atgcgcttgt
     1081 ttggatagtg gtgaaatgaa tttgtgtaaa taataagcgc agagtaaaca agagggaatt
     1141 tagaagttag acaagttaga atagcgtcta gattaatcat gagcagctac taagggatct
     1201 agactttgac gcaaactcat ggaataagga gtaggtcttc tcgtaatggg gaggacgcac
     1261 aagaaattgt gtcgtgtgct gcagtacatg tagatacttg cgaggacgtt gattattgga
     1321 tcaatcgagc atttaccgct gtctactagg gtttctcgtt tcggacgcca taaacgagtc
     1381 gaaatttttt atgtattttt aagtaaacga aaaacgataa aatatgaaca agtcctgaat
     1441 aaagtgaaaa aattgatata aaacatagag aaaatgaaat ttacgtaatg gaggaatgac
     1501 aaatgaaaaa ttatgatccg aatatacgat tgggcacaca tactattaag gtttctttcc
     1561 aacgttggga ttacaaagga tttgtgacgt ttagaagggg cggaaattgt aaaggattag
     1621 atgttttagc attagacgag gatgatttat acgatcaaac attgactgat aatccaattg
     1681 gattcggctt gctacctgaa gatgatgaag gtaatgaatg gtttaaaatg acgcttatga
     1741 atgataatgg agacgaatta tctgttgaag atacttggtc atatttgagt gattatattg
     1801 tcagctttga aatcattgaa tttgtagcgg ataaagaaga ataaaaactg gaggaataaa
     1861 ttttacagat aaaggcttag aaggagtcac gacgattaat aaaaaggtag tattacctta
     1921 ggcattgagt aaggaat
//
LOCUS       DAYYME010000241         1880 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000241 DAYYME010000000
VERSION     DAYYME010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.247"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(121..204)
                     /locus_tag="ACHBFW_002874"
     tRNA            complement(121..204)
                     /locus_tag="ACHBFW_002874"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(168..170),aa:Leu,seq:cag)
     gene            732..1232
                     /locus_tag="ACHBFW_002875"
     CDS             732..1232
                     /locus_tag="ACHBFW_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9319115.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1422..1766)
                     /locus_tag="ACHBFW_002876"
     CDS             complement(1422..1766)
                     /locus_tag="ACHBFW_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFM9319116.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT          582 a          335 c          300 g          663 t
ORIGIN      
        1 tttttgattt atttgaatct ggttccgttg tttaaaattt ttcctatatt cttttagata
       61 atatgttatt ttctttctca tcgctccagg tgtaccttct ctttataaaa aaacaaatta
      121 tgcggctaag aagactcgaa cttccacgag gtcaccctca ctagcgcctg aagctagcgc
      181 gtctgccaat tccgccatag ccgcatcaca tcttttagtt taggattaca tgtgtttttt
      241 gtcaagaaca tatttttgtt ctttttcatt tctgtacgcc ctcccttatg ccatctacac
      301 aaataattca tccgagcaac tccatcatct attgagggtt ggtgaaaaaa tctgttttat
      361 ccatatgatc gatttcaatg ttaaacgaac gactctggga ttagtaacta ttttattttt
      421 ttcttcattt cttttttcct ctttttctcg attcagctat cttctttcat ttggatattg
      481 attatttctt ctgttgatta ctatccttct ggaattctag acgctgaagt aattcacgtt
      541 ttctttcttc tagagatcat aagtaacgaa tgaacgaact tcaaataatt tttttattgt
      601 aattcatatt tgtattgttc gatgattctt aatacgatat gacacaataa ataaaaatag
      661 gcatggctat ttacaacaat agtgctatag tagaaaacaa atgaataagg ttacttaaag
      721 aggtgaatac gatgaaactg atcaaactat gggaagcaga tttaaagaaa gcttatcagc
      781 tacaaactag ctttggacca aatgagagtg gatttatcaa caatgcttat ggctatacat
      841 ttgctgaatt tttagattat gtaaaaaaat gcgaaaaaac gtctttagga atcgatcttc
      901 ctgagagtta cgttcccgca accgtcttta ttttagaaag tgatgaacat gagtatgtag
      961 gaatttttaa tttgcgccat aaactgaacg aaacattaag aaacggcgct ggccatattg
     1021 gttacggcat ctcccctaat tatcgtaaaa aaggatatgc cacacaagga ttagcattag
     1081 tactacaaga ggcaaaaaaa atcggcattg atgaggcact tttgtctgta aataaaaaaa
     1141 atcttggcag tcttaaagca caacaaaaaa atggggctgc gatttatcgt gaagatgaga
     1201 aagaatacta tacgaaaatc aaactgatgt aaaaaagaag gattccaaaa ggaaagggat
     1261 ttgaatgtcc tttatcaata gaacacattt atttaattaa cttataatat agcagacaaa
     1321 attttataaa caaagaaacc ttttatctct tttcttctag aaaggataaa aggctttttt
     1381 gtttttccta tttgcttctc atagctagtc agccaagatt tctatcgtat ccccaacgca
     1441 atccgtgcat aacggctcat cttatctgtc gtccatgctg gataccagac tagtttgact
     1501 tctgctttcg ttacttctgg cacttctttt aatgcgtcat ggatagattc tgttaaaata
     1561 tctgctaatg gacagcccat agttgttaga gtcatcttaa tgactgtttc tccattttca
     1621 ggattgaatt ctatatcata aatcaagcct aaattgacga tgtcgattcc taattcagga
     1681 tcaatcacca tttccaaggc agcaagaata cgttctttga ttagttctat ttcttgtcca
     1741 gaccgttctt ggtttgcttc gctcatcgta cgacactcct taacacgatt tttatttatc
     1801 atactctttt tttagctgct tgtaaatgag aatttagaag taagattctc gtttaaattt
     1861 ataaaaatgt tcttattaga
//
LOCUS       DAYYME010000242         1788 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000242 DAYYME010000000
VERSION     DAYYME010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.248"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1552
                     /locus_tag="ACHBFW_002877"
     CDS             <1..1552
                     /locus_tag="ACHBFW_002877"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFM9319117.1"
                     /translation="NLRSKMDASEYKNYLLGLIFYKYLSDRLLEQVVLLADESLEEYD
                     TVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLADQAKQATFQLNDLN
                     KAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVEVLGHDGDVI
                     GDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSL
                     MLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTD
                     EPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETG
                     TMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQ
                     TRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLANFDEIKENDYN
                     LNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAISSLQTTPENEAWLQG
                     ALEVFKHE"
     gene            1545..>1788
                     /locus_tag="ACHBFW_002878"
     CDS             1545..>1788
                     /locus_tag="ACHBFW_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM9319118.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELI"
BASE COUNT          622 a          295 c          376 g          495 t
ORIGIN      
        1 taatctccgt agcaaaatgg atgcgtcaga atataaaaat tatttattgg gactgatttt
       61 ctataaatat ttatccgacc gtttgttgga acaagtggtt cttttagcag atgaatcctt
      121 agaggaatat gatacggtaa gcaaacaaac gatgttgtat cgcgaattac tttcagatga
      181 agaatcaaaa gaagatctga ttgcaacaat cgttgatatt ttggggtatg cgatttcgcc
      241 agagtatctt ttcaacgtct tagcggatca agcaaaacaa gcaacgttcc aattgaatga
      301 tctaaacaaa gcctttgtgc aattggcatc tacctacaat caatttaatg ggttatttga
      361 tgatgtggat ctccaatcaa aaaaactagg aacagatgag caacaacgaa atgtgacgat
      421 taccgaagtc attaagaaat tgaatgacgt ggaagtttta ggtcacgatg gggacgtgat
      481 cggggatgct tatgaatttc tgatcagtca atttgcttca gaagcaggga agaaagccgg
      541 agaattttac acgcctcata tggtttctga tatgatggca caaatcgtga cgttggatca
      601 aaaagaacga cgattcttta gtgtttttga tccaaccatg ggttcaggtt cattgatgtt
      661 aaatgtgcgt aactacttaa ctcacccaga taacgttaaa tatcatgggc aagaattaaa
      721 tacaacgaca tacaatctag caaaaatgaa cttgatttta catggggtag atgccgaaga
      781 aatgaatctc cgcaatgggg atacgttgaa taaggattgg ccaacagatg aaccttacac
      841 atttgatgca gtagtaatga acccacctta ttcggctaat tggtcagcag atacgacctt
      901 cttagacgat tctcggttta atcgttatgg aaaattagcg ccaaaatcca aagcagattt
      961 tgcgtttctt cttcatggtt tctatcattt gaaggaaaca gggactatgg ccatcgtctt
     1021 gccacacgga gttttattcc gtggcgctgc agaaggggtc attcgtcaaa aattactaga
     1081 agacggcagt atttatgctg tgatcggtat gcctgcgaac ttgttcttcg ggacatcgat
     1141 tccaacgacg gtgattgtat tgaaaaagaa ccgccaaact cgagacgtat tattcattga
     1201 cgctagtcga gaatttgtca aaggaaaaaa tcagaataaa ttatcggaag aaaacattca
     1261 aaaaatcctc gaaacctatg ctgaaagaaa agatgtcgag aaatacgctc atttggcaaa
     1321 ctttgatgaa atcaaagaaa atgactataa cttaaatatt ccacgctatg tagatacgtt
     1381 tgaagaagaa gaaccaattg atatggttca tgtcggaaat gacatcaaga agatacgaca
     1441 agaacagcaa gtgctagaaa aagaactttt agaagctatc tcttccctgc aaacaacacc
     1501 tgaaaatgaa gcgtggttac aaggtgcgct ggaggtgttc aaacatgagt aatgatatac
     1561 aaccagaaat tcgtttccca ggatttacgg aagcttggga acagcgtaag tttgaatgct
     1621 tacttgataa aaaagatgga gtaaggaggg gaccatttgg gagtgcctta aagaaagaat
     1681 tctttgtatc taatagcaat tttgttgtct atgaacaaca aaatgctatt tatgataatt
     1741 acgaaacaag atataaaatt actgaaaaaa aatataatga attaatca
//
LOCUS       DAYYME010000243         1763 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000243 DAYYME010000000
VERSION     DAYYME010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.249"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1604
                     /locus_tag="ACHBFW_002879"
     CDS             <1..1604
                     /locus_tag="ACHBFW_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFM9319119.1"
                     /translation="ETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHK
                     MFRPDNAYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPI
                     REGRTMEDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYN
                     GDINNRLARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFS
                     RTNRTYPEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQA
                     YGKLKEVSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQ
                     IQLLEEQIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTY
                     AANSSDAREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQAS
                     DNLIHAFSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFK
                     YPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          640 a          283 c          357 g          483 t
ORIGIN      
        1 tagaaacata tgcagagtat tcaccggaac agatcaatcg tatgatcgat caaatggagt
       61 ctgtcaaaaa agaaagttat ttggacccta ctgtttttga agcagaggaa catatccaaa
      121 aagtcattca taaaatgttc cgtccagaca atgcctatac aaaatttgat tttcgaaatg
      181 gccgtccaac gaagtctgcg atcttaacca cgagttccat tgatatggcc aaaaagtatt
      241 atcgggccat caaagagatg acgaaagaat ctgattggtt aataaaagag tttcccaatc
      301 agcctatccg tgaaggacga acgatggagg atcctgattt cccacgaatc gctattacct
      361 attcattaga agaaaatagc cgagattcaa gtgcccaaca agaagaaatg caaaaaatta
      421 ttgaggaata taatcagtat tatgatactg cctggtcact agcagacatt gagcgttata
      481 acggagacat caataatcgt ttggcacgaa aaagagcgga attcaaacaa tttgggaaac
      541 aaatcgattt agtaatcgta gtggatcgtc tattgacagg ttttgacgcc cctacgattc
      601 aaaccctatt tgtggatcgt aatctagagt atgcaggatt gatccaagcc ttttcacgta
      661 cgaaccgtac gtatcctgaa aaaacaaaag gactaattgt tactttccga aagccagcga
      721 caatggagaa gaatgtcgag gatgcgacaa aactgtattc agaagctaaa gaagaatcgg
      781 gtttgattta tccaagttac caagaatcca aaaaaagatt caagcaagca tatggtaagt
      841 taaaagaggt ttcatccatg gaatccattg atgaacatac gccacttgaa atccgtatcg
      901 agtacgtcaa agcatttcaa gaattgaatc gtgcgtatga atcgttagtg acttatgatg
      961 attacaatga tgacatggtg caatcttcaa ctttgaacaa tcaaattcaa cttttggaag
     1021 aacaaattgg cgtgtacgag accgtgaagg gttcattgat ggaggaagaa ccagaaaaag
     1081 agggagaaga tttttcaacg atcgaatttt atagtgaaaa taccactaag ttgtatgata
     1141 ttgattctac ttatattgat caattactag gaacttatgc tgcgaatagt tcagatgcac
     1201 gagaagaaat tgaaaaagct ttggctaaat tgaataaaac agaaggagtc aaagaagttt
     1261 accgacaaat tttaaatgca tttgatgtgg ggactttaga tagtaatgaa gatatctttg
     1321 tcattaaacg tcgctatttt acacaagcaa gtgataattt gattcatgcc ttttccaatg
     1381 agtggtttgt ttctgagaaa gaattacatg cttctaatct gcaatatatg ccaggtgaag
     1441 atccaattcc taatatgaaa gcaattatca acagtaagga ttacgaagga tataaagcca
     1501 aacatccaga agcgaaacca tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa
     1561 tgttagatga tgagttgatt ccacttgaaa atgaattgag gtaaacgaga agagttaaga
     1621 aaattgtacg attcaaagat agtagtaaag tggaagtgta gaaaataggg atagatgtct
     1681 ttttacttgt tcacatttta taacggagta tttaagaaag ctattgaata atgattcttc
     1741 catctttttc attcatttac cgg
//
LOCUS       DAYYME010000244         1721 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000244 DAYYME010000000
VERSION     DAYYME010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.250"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACHBFW_002880"
     CDS             complement(<1..182)
                     /locus_tag="ACHBFW_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9319120.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACHBFW_002881"
     CDS             517..951
                     /locus_tag="ACHBFW_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM9319121.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACHBFW_002882"
     CDS             974..1294
                     /locus_tag="ACHBFW_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319122.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACHBFW_002883"
     CDS             1435..1626
                     /locus_tag="ACHBFW_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319123.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
BASE COUNT          651 a          258 c          362 g          450 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa a
//
LOCUS       DAYYME010000245         1687 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000245 DAYYME010000000
VERSION     DAYYME010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.251"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..137)
                     /locus_tag="ACHBFW_002884"
     CDS             complement(<1..137)
                     /locus_tag="ACHBFW_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9319124.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     I"
     gene            complement(134..787)
                     /locus_tag="ACHBFW_002885"
     CDS             complement(134..787)
                     /locus_tag="ACHBFW_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9319125.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1075..>1687
                     /locus_tag="ACHBFW_002886"
     CDS             1075..>1687
                     /locus_tag="ACHBFW_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM9319126.1"
                     /translation="MDNHTRKLLVLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          540 a          307 c          232 g          608 t
ORIGIN      
        1 tgaatctttt cttttctcgt ctccaaatat gttttaggaa agataaaaaa aattaatgaa
       61 tgaactaata taattattat tccaagaaca gaaaatattt gtatgaacat ttttggaaat
      121 atctttaatt ttttcatttt ctctccaatt tatatcctac atttcttata gtagttatac
      181 agtctaattg taattttttt ctaagttctt ttatatatac atctattact cgatcataag
      241 gagtttcttc gctatctttc catacataat caataatttg cattcttgtt aacacttgtc
      301 cattattatc caataaacat tttaatactt ctagttcttt tgcatttaca tctatttctt
      361 caccatttat tttagctgta tagctattaa aattaaccga tagatcctta tattcaaact
      421 tctctaaatg tccataattc ttcttaatta aagaatctat tctagctttt aagaccggca
      481 atgaaaatgg cttttctaca tatccgtcta ctaaattagt aaatgcatca attttatatt
      541 cttcatcact aaatgcagtc aagattagaa ttggtaaatt gcttttcttt ctaatttctt
      601 tcaacacttc taaaccattt ataaaaggta tctgaatgtc taagataacc aaatttatat
      661 cgctattaaa ttttgacaag gcttctcttc catctttagc ttgaataaca gtatatccaa
      721 attctgaaag atattcactt attccctctc ttatcatatt atcatcttca acagttaata
      781 ttttcatata tacctccact taagcctata cactagatat tatacagcat attatggctc
      841 tataatatct agtgttggtg acaccttctg ttgccaatac tttttctttt ttctaataaa
      901 tgcaaacaga caccttgaaa tcctttagaa taaagggttc tataatattt tgtgttgttg
      961 gaaaatcgaa ggcgattttc tactaacact ttttcttcgt ttcaactcaa aaagagactc
     1021 acttttggta aaatttagtc gaccaaaaca tcaccctacc aaaggagtcg ctctatggat
     1081 aatcatacta gaaaattact cgtattaaca gataaacatt tattttttga tgaagaatgg
     1141 ctgattgaaa aagaatataa aggcgttcaa gcgcagttta ttaaagggag attggatgac
     1201 tcaccttccc attgtgaaca ctgtggatcg attcgtatta tccgcaacgg ttcctatacg
     1261 acacgtactc agatattgaa agtcaaagaa aagttgacta ttttagaatt aaaacgtacc
     1321 cgttttctat gctatgattg tgggcagacc ttttctgcga aaacggactt agttgatgaa
     1381 catcatcaac taacaaagga actgaagcag gcgattctca tagaactgta cgaaaatcaa
     1441 tcacgtaaac taatcgctaa gaagtacttc gtctcggatg ggacggtgac acggatcttg
     1501 cgtgaagcaa cgaagcatta ccagccacgt atgaacttcc ttccgacggt gttgtgtatg
     1561 gatgagttca aatcaatgaa gtctgtttct ggttccatga gttttatttg tgttgatggg
     1621 acaacccatc agctatttac gatccttgaa gatcgcagac tttacaaact aactcaatac
     1681 ttcctgc
//
LOCUS       DAYYME010000246         1644 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000246 DAYYME010000000
VERSION     DAYYME010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.252"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            165..587
                     /locus_tag="ACHBFW_002887"
     CDS             165..587
                     /locus_tag="ACHBFW_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319127.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            597..800
                     /locus_tag="ACHBFW_002888"
     CDS             597..800
                     /locus_tag="ACHBFW_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319128.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1011..1595
                     /locus_tag="ACHBFW_002889"
     CDS             1011..1595
                     /locus_tag="ACHBFW_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9319129.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          588 a          228 c          296 g          532 t
ORIGIN      
        1 tttttaaatg gcaatatttt ttgtaaataa atgtcaatca agaaaattta aacgattatg
       61 acattaaaaa tatattttat gaattttcta gtatataact tgatttatct gttaagattt
      121 attaagaaaa cgttttctta tttttgattt cgaggtgata gacaatgaaa aataagttta
      181 ttttatcttt aggtatttta actattggaa ttagtgtaac tggaactaca attcctacat
      241 ttgctgatag tacaaatgaa ccaactactg tagtagataa taaccaagaa ttaactcctt
      301 tagataggaa cggaatagcc attggtaacc caactaacga cttaactgtt actgaaaatt
      361 atttaatagt ttctccatta cttgctcgag cagcttctaa aacgatacga gtgacaggaa
      421 cgatgatttt tgataaagtt ggttggcctt ctagacctcc tcaatcacgg aattggaaag
      481 aaaaaagaaa tggttattgg tactatggaa aattgtattt acaaagttat aaatcagctg
      541 gaaacaattg gattgcgact tattcaggaa ccttaactag aaagtaggga ttttttatgc
      601 cagaatttgt gaacacacca aatttttatc aagatagcga tattctaagt aaaattaatt
      661 taccgaaaga agaatcagct ttacaagttc agcgaacttt acaggagaca caaaaaattc
      721 aaaaatcaat ttctaatcta gaagattctg aagaaaaaag aaaaaaaaga gaccaaaaaa
      781 catatcaaaa agaaaaataa tgattttaaa aaaagagtgt ctagtcgaca ttcttttttt
      841 ctaattgaaa gtgttcataa aatcacccat ttctggacac tttctttttt tgctttattt
      901 ttcaaaaaaa caattcattt catgttcata aacccataac aatatctatg ttcagttaaa
      961 ctatatggat aaagttatgt tacaataaaa ttaatgaagg agtgattttt ttgaaaatag
     1021 gatacgcccg ggtatctact ggtttacaaa atttggattt acaagaagac agcctaaata
     1081 aatttggttg tgaaaaaatt tttactgatc atatgagtgg ggcaaaaagt aatcgaccag
     1141 gattagaaat ggccattgat tttgttcgct ctggcgatac acttgtcgtt tggcgcttag
     1201 atcgtttagg tagaaatatg gaagatttga tttcaatcgt caatcgactg aatgagcgtg
     1261 gtgtgagttt tcatagtatt caagaaaata ttacgatgga caaatcaagt tctacaggac
     1321 agctcatgtt tcatttgttt gcggcatttg cagaatttga gcgtaatttg atcctggaac
     1381 gatccgcagc aggacgagca gctgctcgtg caagaggacg gtttggtggt cgtcctgaaa
     1441 aattgtctga acaagatttg gaattactga agactttagt ggatagcggt acacctatta
     1501 agactattgc ggaacggtgg aacgtctcaa gaaccaccat ttatcgttat ttggcaaaaa
     1561 tggaggagaa aaatggaaag acaaattctc attagttttt gtcatttaat gctgaaaatt
     1621 tttgccattt atctagaaaa gtga
//
LOCUS       DAYYME010000247         1617 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000247 DAYYME010000000
VERSION     DAYYME010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.253"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..1617
                     /locus_tag="ACHBFW_002890"
                     /pseudo
     CDS             23..1617
                     /locus_tag="ACHBFW_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          504 a          262 c          293 g          558 t
ORIGIN      
        1 gaaaggagta tataattaag taatgcaagt aaagaaaaaa ataatattgt ccattctcct
       61 agcaattatt gctggacttt attcaatcaa ttattttaga aatgttcata ctatttctac
      121 atcgggggat attgccttcc attttgctag agtcaaaggg ttatcttcaa ttttttctgg
      181 tccaatcaat tttacgacgt ttaatcatta tgggagcgga gtgaattatt tttatcccta
      241 cttgaccttt tttccggcag tcatttttta ttggataagt aataatttaa tcgttagtta
      301 tatcttatat gtttggctat tgaatgtttg tactatcatg ctgatgtttc attatggact
      361 aaaattttta aaaagaatcg atgcggcttt tatatttagc tgtttatata cattttatgg
      421 gtaccgtacg attgatattt atcatcgttc tgctattgct gaagcgattg cacttacggt
      481 tataccgata gttatgtatt atgcttacgc actgatttat gagaaaaagc catgtgcaat
      541 tccattagca gtcagtatga gcttactcat ttatacacat gttctttcca cttttatgag
      601 tgtaatagcc gttggcttac tagtcttagg cggctttgta gtaagtgaaa caagaaaaaa
      661 agaaagcatc cggttgattg tggagttgtc aaaggcagta ggaatgacga ttcttttaac
      721 agcatacttt tggtatccga tgttccgaca aatcatggtt caatcgatta atgagccaga
      781 taaaacgacc ttacaacacc aagcattaaa tgtctcggat agtttgattg gtgcgatgaa
      841 taatgacctg acaaccttta cggtaggaat cattggtatt atttctttga tctgtccact
      901 cttctttttt aaaggattta atcgcaaaga aaaagtgatt taccttagtg cggtagtctc
      961 ttggctctta tccaccaaat tttttccatg gtctttatta caaaatacac ccattcaaat
     1021 gattcaattt ccttggcgaa tcttaggctt tcaagtactg tttggctcgt tgattttatc
     1081 gttcgttttc atgaagagca catcagctaa gcaaagaaaa gtagttaata taacggcaat
     1141 tgttttatta ctgttggctg tatctgctgc aacaaaagca aactatgaac ataaagttgt
     1201 cactcagaga gatcacttgg taatgaatca agaaaatctt attcgttaca cgacactcct
     1261 tcccggagga ctttatgact atgcacccac ggaagcatta caatacaaag aacatttgca
     1321 gaatcatgaa gtaagaattg gcaaggaatg gcatactatg ccttatcaag tgatggattc
     1381 gaaaatagtc tatacagtac atgaatctaa aggacaaaaa ataacgcttc ctgtatttga
     1441 ttattgggga acagtcgcaa aagtaaatgg acaaaaaaga gagattgata ataggactag
     1501 tgtcgattga aggaacacaa ggagaaacaa tcattgaagt ttcttccatt tatcctccaa
     1561 ctatgaagtt agctttttta attagtttat gtagttacct ctttctttta attagtt
//
LOCUS       DAYYME010000248         1609 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000248 DAYYME010000000
VERSION     DAYYME010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.254"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..542
                     /locus_tag="ACHBFW_002891"
     CDS             144..542
                     /locus_tag="ACHBFW_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM9319130.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1220
                     /locus_tag="ACHBFW_002892"
                     /pseudo
     CDS             526..1220
                     /locus_tag="ACHBFW_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
BASE COUNT          614 a          234 c          313 g          448 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcagcgataa
     1081 cgttaggtaa agagctacta gatcaagtat tagagatgcc ctcgattgat aaagcagtga
     1141 ggggcttcca agaagattgg gtgcaaactt tccaagcaga aacgattggt gaactaaaaa
     1201 aggtcgctga ttacttctga atcaatttct ccataaaata aacgatagcc ttctcgaaaa
     1261 ctcgaggagg ctatctgacg attcaatatt cattaattta aagtttgggt gactattact
     1321 aactattact tatttctcat ttaaacgatg tattccctaa cctctaatac atccttgtaa
     1381 ctctgaagtt acgccagttt tgaataaatt tcataaaaac aaatgaaaag tgtcgcataa
     1441 aaatatcttt cgtgtttctc tagtataaca gaataagaag aacaagtcca gaagggatat
     1501 ttttagtctt ttttaaaata acttttcagt cgagtttgag tttatgatag ttgtttttga
     1561 agcgatacgg caagtgaaaa aatagggaag catgaaagaa tctacttaa
//
LOCUS       DAYYME010000249         1583 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000249 DAYYME010000000
VERSION     DAYYME010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.255"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..231
                     /locus_tag="ACHBFW_002893"
     CDS             <1..231
                     /locus_tag="ACHBFW_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9319131.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
     gene            390..>1583
                     /locus_tag="ACHBFW_002894"
     CDS             390..>1583
                     /locus_tag="ACHBFW_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9319132.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNN"
BASE COUNT          509 a          306 c          294 g          474 t
ORIGIN      
        1 ttaaaaaatc ttcctgaaac acttgatgca caacttcgca cgaaacttca aaacctttta
       61 acgtatgaag aaggtattta caatgctatg atttacccgt attctaatgg caaaatagag
      121 gccaagattc cccatatcaa gacactgaaa cgattatctt atggatttaa atcatttgag
      181 aacatgaaaa tcaggatttt tttgatcaat caattgattc aagtgaaata acaaaaaaat
      241 ctgagacaga aggtcagtcc ttctatcggc tctatacttt ttgatgttgg tgaacttcac
      301 caacatcttt tttgtataaa aaaatataga aaagacaccc tgaaatcctt tagaattaat
      361 ttggctacca attcatagga ggattcaaga tgtctcatac tcagcttatc aaagataccc
      421 taaatattct agacctaaat atctatttcg aggaaaattg tttaactaaa gaaaaataca
      481 aagggcaaat ttgcttgatt tacaaaggaa ctcttctgta caagccagaa gagtgttccc
      541 attgtctttg tgtcgtcccc tctaggatca ttcgttgggg gacgacaacc gttcggttgt
      601 tactgaatga tgtgtctgag tatcgcactt atctggagct gaagaaacaa cgatttaaat
      661 gtaagtcttg ccaaagaaca ttcgtagcgg atacctctgt tgctgaaaaa cactgtttca
      721 ttagtcaaaa agttcgttgg tctgtcattg ctcgcttaaa agagaatact tccatgacgg
      781 aaatcgctcg tcagaaaaat atttccactt cttcggtcta tcgtgtcatg aagcgattct
      841 accgcccatt gaatcctttc aaacagactt tgccaaaggt cttatgcttt gatgaattca
      901 aatcggttcg aagtgttgcc agtgcaatga gttttatcat gatggacgga caaagccatg
      961 cattattgga tattgtcgaa aatcgacgct tgccttattt agaacgctat ttttctcggt
     1021 tttcattagc gactcgtgaa gccgttcaat tgattgttat tgatatgtat gcgccttatg
     1081 tttcgttggt gaaaaagctt ttcccgaatg ctcagttgat catcgatcgt ttccacatcg
     1141 tccaacatat tggcagaact ttcttgaatc atcgagtgaa agaaaccacg gtacgactta
     1201 aaggaaattc ccactctcaa cgagggttag ggaaaaaatt gaagcgctat tggaaactcc
     1261 tacaaaaaga agaagcaaag ttaaactatg aaaaacgggt ttggcgtgcc agttttaaag
     1321 attatttgac tgaaagtgag attgccgatc gactgttagc tgcttgtccc aacttacgcc
     1381 aaggctatca gttataccaa gacattcttt atgcggtaaa gaaaagagat cagtcactat
     1441 ttgaggattg tttaactaga gacattagcg gtctgccaga aacctataca accactttac
     1501 gaacgtttaa aaagtttctg ccacagatcc aaaatgcgct tcactacagc tattcaaacg
     1561 gaccattgga atgcttaaac aac
//
LOCUS       DAYYME010000250         1554 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000250 DAYYME010000000
VERSION     DAYYME010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.256"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..1172
                     /locus_tag="ACHBFW_002895"
     CDS             3..1172
                     /locus_tag="ACHBFW_002895"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM9319133.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          559 a          271 c          252 g          472 t
ORIGIN      
        1 ttatgaaaaa aggaaaacat gctaaaaaaa gaaaatattc atttttaatg atcgttgtcg
       61 tgttgtttag ccttagtgcg ttctctacag gaactattct tggatggcaa ttttacaata
      121 atgaacactc tcagatagtt gcccatgctc aatcagctca aaaaaagttg atactgacta
      181 ccaatgaaca acagctcgtt gaaaaaatcg atcaactatt aagaaaaaat gattatattg
      241 gctcaattta cgtaaagaaa aataatcgca tcatcatgga aaaaggctat ggctatgcga
      301 ataaactcac gcaacaacca aatgatcctt ctctttatta tcagattggt tcggttcaaa
      361 aagcattgac tactcttttg attttaaaac aagtcgagcg tgggcacctt tctttagata
      421 ctaagctggc tcaattctat ccacaaattg ctggcagcca gcaaattact atccgtgact
      481 tgctgtatat gcgctcaggc ttgcaccgaa ctgcttctcc aaccataccg atgactgatg
      541 aagaagtcgt tcaatttgcg attcagcact taaaatttac agattatcag acctctcatt
      601 atgattcatt gaactttacc atattgactg gaattttgat caaattaaca aatcaaccgt
      661 atgaaaaatt attgaccgat gatatcatcg aacctttagg attacagcat acaaaatttt
      721 atgaccaagt caaaaattca tccaatcatg cactatccta tcaaatgtct aaagaggatg
      781 attactttaa accactgaca gaatctgaaa cagagatcca taacgaatta gggactggta
      841 atatcagtat gtccgtctat gatttaaata cgctgtttac acaagtttta tctggaaaac
      901 tagtttccaa agacttactc ttctcattat ggcgtgaaaa tgcgaatgga catccatata
      961 gtggtggggt atatagcgga gataattata tcctgtcgca aggaaatatc gactcttttg
     1021 atacatctgc tatatttagc aaagattcac aggatgctat catcatggaa agcaatataa
     1081 aagctgacaa aactatcaaa ctgcctactg ctgatctaag aaaccaaatc tatgacctga
     1141 tggaaggaac tactaagttg gcaaatagct gatatgatga aaagatagca aatactactt
     1201 ctttgaatag catttgctta aaaaaagtac tcgactaaac gtaggaaaga gtgtgggaca
     1261 aatctaaaaa tcaatttttg ctacagactt taattccaaa taaacagaac agaaagaggt
     1321 gttcttcttt ctgttctgtc caaattattc taagatttct ctttaccaac attatttgac
     1381 aaagggccta ttaaatctaa aatagtatct ttagattgct aaaatatgtg ctagcaaatc
     1441 attcatggct tgttcttatt tttaatttta tgcaataaaa aagaagaaca attagcgtga
     1501 ttatagaaat aaataatgag attttagaaa aaaatgtgta tgaatacctg attg
//
LOCUS       DAYYME010000251         1542 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000251 DAYYME010000000
VERSION     DAYYME010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.257"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..>1542
                     /locus_tag="ACHBFW_002896"
     CDS             86..>1542
                     /locus_tag="ACHBFW_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9319134.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVSICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNR"
BASE COUNT          558 a          214 c          282 g          488 t
ORIGIN      
        1 catcataaaa tcagtatagg tgaaaagatg aactttcatc tgcatatcaa gtaattggta
       61 tgaggcattt tttgaggtgt aggatatgaa gttggattac cttatagaag aggaagatag
      121 agaaaaactg ctgctagcta gatatttaga atttgagaaa aagaattatg tagtgctgcc
      181 taagttatat gaaacattag gtttgtctaa atttaaaaca agaaattata tcatagaatt
      241 aaataaagaa tttactgaat tcaaaacaaa gccagagatc caaatttcag aagaagcaga
      301 gatccatgtc gaacgtctga atttgacgga tatcaaaaat ttaagaagaa tctactttca
      361 acgatcaaaa attgctcgac tgttaacgga gatcgtggat tcaaaagtaa cgatcgaaaa
      421 atttgcagag aatcattttt taagcatatc aagagcctat gtaaaaagaa gagaattagt
      481 ctcttttctt gaacagcatc aaataaagtt aaagaaaaac atattagttg gggaagaaat
      541 aaacatacgg aacgtattat ttggggtata tttcggggtc tttaatggtt tttctatgcc
      601 atttagagaa agcgtcatga atgaagcaaa gagtatcgtg caatattttg aatatctgtt
      661 caatttaaaa ctgtctgcaa caaaacgaat caaattcaat ttgctaacca ctattagttt
      721 gttgagggcc tatgggaatc aaccaatcgg aaaacgtttc ttttcagatg aatactttaa
      781 gagctcagaa ggaatgaaga tcatcaagga tttatcacat atttcttttc ttcatatccc
      841 taaagagcag atcattgatg agatttctta tatcatgttg tttttgatca cggaagaaga
      901 atttgaatta gatggcgcat ttggacttgc ggaagctcat tttcatgata tccacatgat
      961 tagtcaaaaa atgtcagtgg aattgattga tcaattacca ttttcgattg aaatctctga
     1021 agaagaaaga ctatatatac gaaaagaact tcgtatgggt atgatgcatg tcaatcgaaa
     1081 aaattggata ttcgactttg agatgactag ctttacgtct aaaaagcagc tacagttttt
     1141 cttcgagacg tatccaatat tcagtacagc aatccaaaag gtcatacaag agaataaacg
     1201 ctatttcttg ggaaaagatg aacgtttcat gattcgctta ttttatgatt atctcttttt
     1261 attggtcagt atttgtccgg tatcatattt tgaagcaccg attcatgtct gtattgattt
     1321 ttcgcaagga tctgcttata ccgattacat tatttctcaa gtgaaaggat ttaaaaatta
     1381 caatattgtg attgagagtc gaatcacttc tcgtacgaat atttatttat ctgattgttt
     1441 acttgaaaaa tttaataaag aacagataat ttggaaaaat ccaccgactc cagatgactg
     1501 ggaatatttt ggaaatacca tcatcagaga aaaaaataga ca
//
LOCUS       DAYYME010000252         1537 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000252 DAYYME010000000
VERSION     DAYYME010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.258"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(89..1219)
                     /locus_tag="ACHBFW_002897"
                     /pseudo
     CDS             complement(89..1219)
                     /locus_tag="ACHBFW_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     misc_binding    complement(1279..1506)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          434 a          309 c          244 g          550 t
ORIGIN      
        1 tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg actttttggg
       61 acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg acttgctgca
      121 ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct tctgttgaag
      181 caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt tgtgcttctt
      241 ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa gtaactaatc
      301 ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa tctcctgctc
      361 ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac tcttctgcaa
      421 tgggttcata tccaccggaa aacagctaag aagaagcgtg attcccgcgg cagatatgtg
      481 ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc tctgtacaaa
      541 tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt ttagtttgat
      601 cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct ttttcataaa
      661 aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca gatagatcag
      721 gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt cgtaaaatca
      781 tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc actacttctt
      841 tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat ttatctacta
      901 cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat ccagtttctg
      961 gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa aatccacgcc
     1021 gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc actaattttt
     1081 ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct tcttgaactt
     1141 tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga tctactgtaa
     1201 atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa aaaaaatcct
     1261 tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt ttttcagaaa
     1321 aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga aagactgttc
     1381 gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt caacttctag
     1441 ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc ttctcatcgg
     1501 atactattag aataaaatta gaatagcatg tttgaaa
//
LOCUS       DAYYME010000253         1516 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000253 DAYYME010000000
VERSION     DAYYME010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.259"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHBFW_002898"
     CDS             131..505
                     /locus_tag="ACHBFW_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM9319135.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHBFW_002899"
     CDS             complement(619..1248)
                     /locus_tag="ACHBFW_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9319136.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHBFW_002900"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHBFW_002900"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          513 a          229 c          264 g          510 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttc
//
LOCUS       DAYYME010000254         1500 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000254 DAYYME010000000
VERSION     DAYYME010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.260"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1455
                     /locus_tag="ACHBFW_002901"
     CDS             <1..1455
                     /locus_tag="ACHBFW_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFM9319137.1"
                     /translation="QDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDTVANG
                     GLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIVLALP
                     FGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGALLAP
                     LIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEERKGFI
                     RNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVTAEEK
                     PRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNPIFIF
                     ILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPLWLLG
                     SFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFYTPGT
                     ESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          407 a          288 c          317 g          488 t
ORIGIN      
        1 caagacaagg gcttcttcgg gcagccgaag ggattgtcaa ctttgttctt cactgagatg
       61 tgggaacgat ttagttatta cggtatgcgt gcattactta tctattatat gtacgataca
      121 gttgccaacg ggggacttgg attaccacga gcaacagcat tagctatcat gtcgatctac
      181 ggatctttga tctacatgtc tagtatcgtc ggcggatggg tatctgaccg agtgttagga
      241 gcaaaacgca ctgtattctt cggtggagtt ttcattatgc ttggccacat cgtcttggct
      301 ttaccatttg gtgtaagtac attgtttgtt tcgatggcat tgattattat cggaaccgcc
      361 tttttgaaac caaatgtttc tggcatggtc ggacatcttt attcaaagac tgacttgcga
      421 agagatgcag gattttcgat tttttacatg ggtgtcaact taggggcatt attagcacca
      481 ctaatcgttg gtacaatcgg tcaagaatat aactatcatt tagggttctc tttagctgct
      541 attgggatgt tctttggatt attacaatac atcatccaag gacgcaagac attagacgga
      601 gtgggtacaa caccgccgaa tcctttgaca actgaagaac gtaaaggatt catccgtaac
      661 ttgatcatcg cacttgttgt tatcgctgca gtattcggcg gggcacaact aactggtcat
      721 ctgacaattg acttttttgt caatacaatc agtgtattgg ggattatctt accgttgtat
      781 tactttatca aaatgctgac ttcaaaagat gtaactgctg aagaaaaacc aagagtatgg
      841 gcttatatcc cattattcct tgcagcaatc attttctggt cattacaaga acaaggttca
      901 tctatcttag ctttatttgc tagtgaacgt acacaatcta cgctatttgg tgtaacgatc
      961 caagcaagct ggtatcaatc attgaatcca attttcatct ttattctgac accagtattc
     1021 gtaacgatgt ggacgaaact tggtgaacgt caaccatcta cagtagtgaa attctctctt
     1081 ggacttttat ttgcaggatt atcgtacttg ctattgatgt tgccgggtat gctttatggt
     1141 acaagtggaa aagtaagtcc attgtggttg ttgggaagtt tcttcatcgt ggttattgct
     1201 gaattgtgtc tttcaccagt tggtttgtct gtgacaacaa aattagcacc acgagcattt
     1261 gaatcacaaa caatcgctat ttggttctta gctgatgctt cttcacaagc aatcaatgca
     1321 caaattgcac gcttctacac accgggaaca gaatctgctt acttcggtat tatcggtgca
     1381 gtagcaatcg tcggtggtat cttgctattt actgtgaaag aaccaatcaa aaaactaatg
     1441 ggctctattc attaatcaat gaacataaaa acaggaattg cttatttgca gcagttcctg
//
LOCUS       DAYYME010000255         1477 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000255 DAYYME010000000
VERSION     DAYYME010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.261"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..1194)
                     /locus_tag="ACHBFW_002902"
     CDS             complement(25..1194)
                     /locus_tag="ACHBFW_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFM9319138.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            complement(1265..>1477)
                     /locus_tag="ACHBFW_002903"
     CDS             complement(1265..>1477)
                     /locus_tag="ACHBFW_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM9319139.1"
                     /translation="NKIVVCIENKILSSESGGQLNKYQIYIERNYSNYKKIFVFLTPD
                     GNEPTNPSWGIITYKNIVEESVKYFV"
BASE COUNT          463 a          253 c          216 g          545 t
ORIGIN      
        1 gaaaagccca ttctgttgat caagttaaaa cattgattct aatgtatcat gtatttcttt
       61 aaaaccttta tgaactcggc atagaaactt ttgattatag ttgttgaatt gagatactag
      121 aaatctctcc agagattctt cattaggaaa ttgctctttt cttttggtat atttctttaa
      181 ttgtttgtta aaaccttcaa tcaaattagt tgaataaata gttcttctga ttgacggagg
      241 gaaattataa aaggttaata tagcaggatt catgagcaat ttaaccactc gtggatactg
      301 ctttttccat ttatctatca taaaccttat ttgattattt gcttcttctt ttgaacttgc
      361 ttggtaaact aatttgaaat cgctacagat ttcttgtcga tcactaacac gaaccttatg
      421 agcgatattt ctagatatat ggacacaaca ttgttgaaat tgagcatttg gataaacact
      481 atgaatacta tcgtcaatac cacttaaacc atctgttacg actaacaaaa cctcttctaa
      541 accacggtct tttagatctt gaagaatctc tttccaaaca taagcagatt cagttggagc
      601 tatagtaaat cctagaacct ctttagttcc atccaacctg ataccaatta ctatatagac
      661 agcttctttt gatacggttt gtctttttaa gggaatatga gtagcatcca taaaaatgac
      721 tgagtattta gcttctaaag ttctttcttt aaatgccgaa acatcttcag acacaatttt
      781 actcatattt gaaatagttt gtggtttgta ataatgacca tacatttttt cgattagatc
      841 agagatttcg gacatagtaa ttcctttttg aactagctga ataatagtgg tttctaaaga
      901 gtcattggtt cttttataag caggtaaagt ttgttgagaa aagtctccat tcctatctct
      961 aggtattact aaattcaatt caccgtactc tgttttaaaa gagcgcgaat aattcccatt
     1021 acgggaatta cctgaattaa atccagctct atcatacttt tcatagtcaa gaaataccgt
     1081 taattcagct tgtaataaag cgttaatagc caattctagg tgacgacgaa acaaatcatc
     1141 caaatcacct ttattgatta gtatttccat aatttctgta gtaaaatcgt tcatatggaa
     1201 gtcctctttt ctgtgaatgt gttgtggtaa ctctattcta cagaagggac ttcctttttt
     1261 ctatttacac aaaatatttt acactctctt caacgatatt cttgtaagta attatgcccc
     1321 aactaggatt tgtgggttca tttccatctg gagttaaaaa aacaaaaatt tttttatagt
     1381 tagaataatt gcgttctata tatatttggt atttattaag ttgccctcca gactccgaag
     1441 acagaatttt attttcgatg cacaccacta ttttatt
//
LOCUS       DAYYME010000256         1454 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000256 DAYYME010000000
VERSION     DAYYME010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.262"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACHBFW_002904"
     CDS             <1..1087
                     /locus_tag="ACHBFW_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9319140.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..>1454)
                     /locus_tag="ACHBFW_002905"
     CDS             complement(1305..>1454)
                     /locus_tag="ACHBFW_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM9319141.1"
                     /translation="AFLIEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEI
                     TRFDI"
BASE COUNT          516 a          256 c          275 g          407 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggc
//
LOCUS       DAYYME010000257         1454 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000257 DAYYME010000000
VERSION     DAYYME010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.263"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..305)
                     /locus_tag="ACHBFW_002906"
     CDS             complement(75..305)
                     /locus_tag="ACHBFW_002906"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFM9319142.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(399..737)
                     /locus_tag="ACHBFW_002907"
     CDS             complement(399..737)
                     /locus_tag="ACHBFW_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319143.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
BASE COUNT          482 a          263 c          181 g          528 t
ORIGIN      
        1 gtaatattaa cagcaaagat atcgaaaaaa caataacata atatgtgtca aaatttttca
       61 taataagaac tcccttactg ttgcatacta acataatcca aaaactcttc tacttcagct
      121 aaagacattc cactttcttt agtccaaaaa acagtttttt ctatcgatcc ttctctgtaa
      181 actgtaaatg tcggagtcga gttaattgag tagcgactct tgaattcttt ccactcattt
      241 tcatcttctc taacttttgc tacattcacg taataaatat tttcttttcc cttttctacc
      301 agcatatcga atagcttcct gcttttgatg atcgatttct tgtgctacat ctagtaaatt
      361 ttcatcaatt tctatatcat aaagtatttc actagtgtct acatccacaa tatatacttt
      421 ttatcagtag caatttttga gtaatccgtt aaaatgatct cagtctcgtc actttcatca
      481 gtataaatat acgttaacaa tgaactcacc ttttcaatcg gtgttagctc taagcctact
      541 gtatttttta ctattgagtc attatttacc accataactt cgtactgtat ataccatcct
      601 atttggtttc catcaatatc agtaattggc tcactagcta atggagtctc cggaacaata
      661 ttttcctgat atttcttaaa ctcttctgta gcacgaaaaa ggttatcatg ttcctcactt
      721 gaaactggtt gagtcataga aaatgttact tggctaaaca gagggaaaat aaacactaca
      781 atcaacaaag tataaaaaaa agcccttctt tttcattttt tatcttccaa tcacaaattg
      841 aaaaacttat tcctgcaact ttgagtctag tatcacaatc gactgactac aactattcat
      901 ttacattcta ctttaagttc ctccttaacc attaatttta ttaaagatac ttgtaatagt
      961 gattctcaca aaatttcatg aaaaatactt tattactgta tctaaaattc caatacacaa
     1021 cttatcgact attatctgaa aaccaaaact tattattcct tgatgttatt ctagcaatag
     1081 taactgcttt tttatttttt tgcatatttt ttcggcgtta ctttttaaag ttaacttttt
     1141 cgacaaatag aaagagatac gaatcactga tttttttgaa gaaaagaaat ggagtgagct
     1201 gaacgttaga gatattggga gctttcgtaa ctgcttatta aacgataatg gttatttgca
     1261 accgtattgc tcgcttctac atgaaatatt tcatgaaata tttcgcaaaa cctttgatta
     1321 tgtagttacg tttccattct tgactaaaaa tgtttcaaaa acgaacaaag gtgttaaaag
     1381 gtaaagtgat caatactact ggtcaaaata agaggttgaa agggaattga aaaataacta
     1441 cagataatac tttt
//
LOCUS       DAYYME010000258         1393 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000258 DAYYME010000000
VERSION     DAYYME010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.264"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1393
                     /locus_tag="ACHBFW_002908"
     rRNA            <1..1393
                     /locus_tag="ACHBFW_002908"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          374 a          300 c          424 g          295 t
ORIGIN      
        1 aagtctgaag aggagtcaaa tgcttctttt cttaaggaca agctgtgatg gggagggaaa
       61 taatagtacc gaagttcctg atgtcacact gccgagaaaa gcttctagtg agaaaacagc
      121 ggcccgtacc gcaaaccgac acaggtagtc gaggagagaa tcctaaggtg agcgagagaa
      181 ctctcgttaa ggaactcggc aaaatgaccc cgtaacttcg ggagaagggg tgctgatcat
      241 acgatcagcc gcagtgaata ggcccaagcg actgtttatc aaaaacacag gtctctgcaa
      301 aatcgtaaga tgaagtatag gggctgacgc ctgcccggtg ctggaaggtt aagaggagtg
      361 cttagcgcaa gcgaaggtac gaattgaagc cccagtaaac ggcggccgta actataacgg
      421 tcctaaggta gcgaaattcc ttgtcgggta agttccgacc cgcacgaaag gcgtaacgat
      481 ttgggcactg tctcaacgag agactcggtg aaattttagt acctgtgaag atgcaggtta
      541 cccgcgacag gacggaaaga ccccatggag ctttactgta gtttgatatt gagtgtctgt
      601 accgcatgta caggataggt aggagccgta gaaatcggaa cgctagtttc gatggaggcg
      661 ctggtgggat actacccctg cgttatggcc actctaaccc gcaccactaa tcgtggtggg
      721 agacagtgtc agatgggcag tttgactggg gcggtcgcct cctaaaaggt aacggaggcg
      781 cccaaaggtt ccctcagaat ggttggaaat cattcgaaga gtgtaaaggc agaagggagc
      841 ttgactgcga gaccaacaag tcgagcaggg acgaaagtcg ggcttagtga tccggtggtt
      901 ccgcatggaa gggccatcgc tcaacggata aaagctaccc tggggataac aggcttatct
      961 cccccaagag tccacatcga cggggaggtt tggcacctcg atgtcggctc gtcgcatcct
     1021 ggggctgtag tcggtcccaa gggttgggct gttcgcccat taaagcggca cgcgagctgg
     1081 gttcagaacg tcgtgagaca gttcggtccc tatccgtcgc gggcgttgga aatttgagag
     1141 gagctgtcct tagtacgaga ggaccgggat ggacttaccg ctggtgtacc agttgttctg
     1201 ccaagggcat tgctgggtag ctatgtaggg aagggataaa cgctgaaagc atctaagtgt
     1261 gaagcccacc tcaagatgag atttcccatt tctttaagaa agtaagatcc ctgagagatg
     1321 atcaggtaga taggtcagga gtggaagtac agtgatgtat ggagcggact gatactaatc
     1381 gatcgaggac tta
//
LOCUS       DAYYME010000259         1364 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000259 DAYYME010000000
VERSION     DAYYME010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.265"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHBFW_002909"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHBFW_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFM9319144.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          393 a          242 c          216 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DAYYME010000260         1362 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000260 DAYYME010000000
VERSION     DAYYME010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.266"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1362
                     /locus_tag="ACHBFW_002910"
     CDS             82..>1362
                     /locus_tag="ACHBFW_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFM9319145.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DAYYME010000261         1337 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000261 DAYYME010000000
VERSION     DAYYME010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.267"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(5..76)
                     /locus_tag="ACHBFW_002911"
     tRNA            complement(5..76)
                     /locus_tag="ACHBFW_002911"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42..44),aa:Gln,seq:ttg)
     gene            complement(86..158)
                     /locus_tag="ACHBFW_002912"
     tRNA            complement(86..158)
                     /locus_tag="ACHBFW_002912"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(123..125),aa:His,seq:gtg)
     gene            complement(220..304)
                     /locus_tag="ACHBFW_002913"
     tRNA            complement(220..304)
                     /locus_tag="ACHBFW_002913"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(268..270),aa:Leu,seq:caa)
     gene            complement(325..395)
                     /locus_tag="ACHBFW_002914"
     tRNA            complement(325..395)
                     /locus_tag="ACHBFW_002914"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(361..363),aa:Cys,seq:gca)
     gene            complement(441..512)
                     /locus_tag="ACHBFW_002915"
     tRNA            complement(441..512)
                     /locus_tag="ACHBFW_002915"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(478..480),aa:Gln,seq:ttg)
     gene            complement(524..596)
                     /locus_tag="ACHBFW_002916"
     tRNA            complement(524..596)
                     /locus_tag="ACHBFW_002916"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(561..563),aa:His,seq:gtg)
     gene            complement(599..672)
                     /locus_tag="ACHBFW_002917"
     tRNA            complement(599..672)
                     /locus_tag="ACHBFW_002917"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(638..640),aa:Trp,seq:cca)
     gene            complement(679..759)
                     /locus_tag="ACHBFW_002918"
     tRNA            complement(679..759)
                     /locus_tag="ACHBFW_002918"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(723..725),aa:Tyr,seq:gta)
     gene            complement(761..836)
                     /locus_tag="ACHBFW_002919"
     tRNA            complement(761..836)
                     /locus_tag="ACHBFW_002919"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Phe,seq:gaa)
     gene            complement(872..944)
                     /locus_tag="ACHBFW_002920"
     tRNA            complement(872..944)
                     /locus_tag="ACHBFW_002920"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(909..911),aa:Asp,seq:gtc)
     gene            complement(954..1026)
                     /locus_tag="ACHBFW_002921"
     tRNA            complement(954..1026)
                     /locus_tag="ACHBFW_002921"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(991..993),aa:Val,seq:tac)
     gene            complement(1042..1113)
                     /locus_tag="ACHBFW_002922"
     tRNA            complement(1042..1113)
                     /locus_tag="ACHBFW_002922"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1078..1080),aa:Glu,seq:ttc)
     gene            complement(1128..1217)
                     /locus_tag="ACHBFW_002923"
     tRNA            complement(1128..1217)
                     /locus_tag="ACHBFW_002923"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1181..1183),aa:Ser,seq:gga)
     gene            complement(1257..1330)
                     /locus_tag="ACHBFW_002924"
     tRNA            complement(1257..1330)
                     /locus_tag="ACHBFW_002924"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1294..1296),aa:Asn,seq:gtt)
BASE COUNT          381 a          352 c          305 g          299 t
ORIGIN      
        1 ataactgggg tagctggatt cgaaccaacg catgagggag tcaaagtccc ttgccttacc
       61 gcttggctat accccaagaa taaagggcga atgatgggaa tcgaacccac gaatgccgga
      121 gccacaatcc ggtgcgttaa ccacttcgcc acattcgcca tcataaaaga tgttatttaa
      181 tttctcaata aaaaactaag atgaagacat cttagtcaat accggcggcc ggggtcgaac
      241 cggcacgccc tcaacgggca ctggattttg agtccagcgc gtctgccaat tccgccacgc
      301 cggcaagttg atttattagt actaaggcgg taaccggatt tgaaccggtg atgaaggttt
      361 tgcagacctc tgccttacca cttggctata ccgccattat actattatca atctataaaa
      421 taaagaataa attatagaaa ctggggtagc tggattcgaa ccaacgcatg agggagtcaa
      481 agtcccttgc cttaccgctt ggctataccc caagaataaa aagggcgaat gatgggaatc
      541 gaacccacga atgccggagc cacaatccgg tgcgttaacc acttcgccac attcgccatg
      601 gcaggggcag caggaattga acccacacta acggttttgg agaccgctgt tctaccttta
      661 aactatgccc ctataaaatg gaggagagtg gattcgaacc actgaaccct aaggaacgga
      721 tttacagtcc gtcgcgttta gccacttcgc tactcctcca tggtggcgcg ggacagaatc
      781 gaactgccga cacacggagc ttcaatccgt tgctctacca actgagctac cgcgccatat
      841 tttaaaatta ttaattgaat aacaagaacg acggttccga cgggaatcga acccgcgatc
      901 tcctgcgtga caggcaggca tgttaacccc tacaccacgg aaccagttca ttatggaggt
      961 taacgggatc gaaccgctga ccctctgctt gtaaggcaga tgctctccca gctgagctaa
     1021 acctccaaca ttcaattaaa atgacccgta cgggattcga acccgtgtta ccgccgtgaa
     1081 agggcggtgt cttaaccgct tgaccaacgg gccaggtaaa tttgctacgg agagtaaggg
     1141 attcgaaccc ttgagacagt ttggcaccat ctacatgatt tccaatcatg ctccttcggc
     1201 ctctcggaca actctccaga gtgaaagaag ctcaatactc tttctcaaaa tgagaactcc
     1261 ggcagtagga ctcgaaccta cgacatcatg attaacagtc atgcgctact accaactgag
     1321 ctatgccgga ataacat
//
LOCUS       DAYYME010000262         1311 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000262 DAYYME010000000
VERSION     DAYYME010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.268"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..578)
                     /locus_tag="ACHBFW_002925"
     CDS             complement(93..578)
                     /locus_tag="ACHBFW_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HFM9319146.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(580..>1311)
                     /locus_tag="ACHBFW_002926"
     CDS             complement(580..>1311)
                     /locus_tag="ACHBFW_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319147.1"
                     /translation="FFDQERYNYELTKRRLQINDLSRFSINVVEVDDYSEITDILKSI
                     RKKYLRKTIFISGSAVDYTPFTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGNSIV
                     SGVLKQRRNLRKNNIQDVLSLRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLESGE
                     IVTADGMIEEFELSVQNENIVVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQALE
                     KLDTEGEVDKKVEQIVSLINSIQED"
BASE COUNT          420 a          268 c          136 g          487 t
ORIGIN      
        1 ttaactgata aaaaaatatc tactatttaa agatacaggg ataagtaagt aaaaaaataa
       61 catgtcatta gatagtagac agttccaact ctttatttac cttttttgta tgctgcactg
      121 tcaatccaag ttcctaaatt gttgtatcca tcatcactta tataatcata ttggccatca
      181 ataatttcgt caaaccacaa atattctcca tctggttttt gcccaattat tttaattact
      241 gattgactag tagttgtcat ctggttaaaa tcttttattt tacctatatt aacggccaaa
      301 atagcatttc ctcgcttata actctcctgg atttcatatt taacatattc tcgatcaaga
      361 gtttctttac ctatcagcac cacagtcact gatgttccaa ttaattgatt atctatccag
      421 ttttttatag caatatctcc attacgttta actttctcaa attcagcctt atctacaaaa
      481 ccagcttcct ttgttcctcg caaagtccag ctgtttctaa ctaccattgc tcttgttaca
      541 tctgcataat ggaaactaaa aaaaaccctt ctcaccatat taatcctcct gaatactatt
      601 tattaaactg acaatttgtt caactttttt atctacttct ccttcagtat ctaatttttc
      661 tagcgcttga aatttacttt ttaaggaatc atcaaaataa tcactgaaat tctcttgaat
      721 cttatcaaac aaaaccttac ttgcaccttt ggtaaatcca atcggaacta ctatattttc
      781 attttgaaca gataattcaa attcttcaat cattccatct gctgtaacta tttcacctga
      841 ttcaagtttg tttccaaaaa caaaaatact aatcccagac tctgcaataa tgttttctcg
      901 atatcttctc cactcatgag gcatatcaag gggaagtggt cttaaagaaa gtacgtcttg
      961 tatgttattt tttcttaaat ttcttctctg ctttagaact cctgaaacaa tactattacc
     1021 aattccaagt ccgtaacctg atactattct ataaccggat tcagaaagcc tatatgctaa
     1081 tttctcgaca aattttaatc ctttcgattc agatgtaaat ggagtataat caacagcact
     1141 tcccgaaata aaaatagttt ttctcaaata cttttttcta atacttttca aaatatcagt
     1201 aatctcagaa taatcatcta cttcaacaac gtttatcgaa aatctggaca aatcattgat
     1261 ttgtagtctt ctttttgtca gttcataatt atatctctct tgatcaaaaa a
//
LOCUS       DAYYME010000263         1292 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000263 DAYYME010000000
VERSION     DAYYME010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.269"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..606)
                     /locus_tag="ACHBFW_002927"
     CDS             complement(<1..606)
                     /locus_tag="ACHBFW_002927"
                     /inference="COORDINATES: protein motif:HMM:NF026247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HFM9319148.1"
                     /translation="MDTEASLEINQEKSVSQKKWSTSNKILSGVLLLIMWIIMAFNTQ
                     NADYYAYSVLYNGIAQFGMDFSSDSIEPGFRAVMYVCTFISSQYQIFLLIISTVLLSA
                     LLYVIRLFTTNVFLVLFLFFIFGFTSQAIQVRMFSALIFILLAFYHLTKLRGSKAIFK
                     YIFFILVAGLFHNLAYFYLPFFIVKINKKITGYVTILSLLLF"
     gene            complement(578..>1292)
                     /locus_tag="ACHBFW_002928"
     CDS             complement(578..>1292)
                     /locus_tag="ACHBFW_002928"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9319149.1"
                     /translation="NIGLATAQNIGIEIAKKEKCDYILLLDQDTIIQKNLSLKLLEEF
                     TNIEAKDKDIGIIVPNYFENSNDESVSFTCKNERGRYVKKHFASTSGSMKIAFANSSG
                     MFFKSELIDDVGNMKDEFFIDQIDVEFCKRVANNGYNIYVTNQVYFYHHVGDGENLKI
                     GSKKIKIDNHNSTRKYFIARNSTLVKRMYKSDKEFSRLMNKRLLKFFIAIAFETDKLS
                     RFRAAIRGVRDGYRGVIGD"
BASE COUNT          460 a          228 c          170 g          434 t
ORIGIN      
        1 gaataggagc aagctaagga ttgtaacata tccagtaatc tttttattaa tttttacaat
       61 aaaaaaaggg agataaaaat atgcaagatt atggaacagt ccagccacta agataaaaaa
      121 aatatactta aatatagctt tgctgcctcg caatttagtt agatgataaa aagctagtaa
      181 tatgaatatc aatgcgctaa acatacgtac ttgtattgct tgtgaagtaa aaccaaaaat
      241 aaaaaacaag aacaaaacta aaaaaacatt agttgtgaat aatctgatga catacaataa
      301 agctgagagt aagacagtag aaataatcaa taaaaaaatc tgatactgac tggaaataaa
      361 ggtacatacg tacataacag ctctgaatcc cggttcaata ctatctgaac taaagtccat
      421 accaaattgt gctatcccat tatacaagac agaatatgca taataatcag cgttttgtgt
      481 gttaaatgcc atgatgatcc acattatcaa aagtagcacc ccagacaaaa tcttgttcga
      541 tgtactccat tttttttgtg aaacactctt ttcttgatta atctccaatg acgcctctgt
      601 atccatctcg aacccccctt atagccgctc taaatctact gagcttatca gtctcaaaag
      661 ctattgcaat aaaaaatttc aataatcttt tattcataag gcgggaaaat tccttatcac
      721 ttttatacat tcttttcacc aatgttgaat ttcttgcaat aaaatacttt cgtgttgaat
      781 tatgattgtc tattttaatt ttttttgatc ctatttttaa attttctcca tctccaacgt
      841 gatgataaaa atagacttga tttgttacat aaatgttata gccattattt gcaacccttt
      901 tacaaaattc aacatcaatc tgatcaataa aaaattcatc cttcatattt ccaacatcgt
      961 caattaattc acttttgaaa aacattccag aggaatttgc aaatgctatt ttcatggaac
     1021 cactagttga agcaaagtgt ttttttacgt atctccctct ctcatttttg caggtaaaag
     1081 agacagattc atcattgcta ttctcaaagt aattgggaac aataatccca atgtccttat
     1141 ctttcgcttc aatattggta aactcttcta ataattttaa agaaagattc ttttgtatga
     1201 tggtatcttg atctagtaaa aggatataat cacatttctc ttttttggca atttcaatac
     1261 ctatgttttg agctgtcgca agcccaatgt tc
//
LOCUS       DAYYME010000264         1266 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000264 DAYYME010000000
VERSION     DAYYME010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.270"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..971)
                     /locus_tag="ACHBFW_002929"
     CDS             complement(<1..971)
                     /locus_tag="ACHBFW_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFM9319150.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHM"
     gene            complement(976..1161)
                     /locus_tag="ACHBFW_002930"
     CDS             complement(976..1161)
                     /locus_tag="ACHBFW_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM9319151.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
BASE COUNT          385 a          242 c          158 g          481 t
ORIGIN      
        1 tgcatatgaa ttggttttac tcctgcaatt tcagctagtt tttttattct gcttaaaata
       61 gttcttggag atggcggtaa tccatcaaaa gaaaaaacca attctatatt tttcccaatt
      121 gattgttgag tttttttcca tgtttttaaa tcttcaacgg tttgtctgtc taacgaaatt
      181 atccgaatac ttgatgtatt ttttgtttca gcaaattcat aatcttgtct tgtacggatg
      241 taaattgact tatttacgct acatctgcca tttattaagt caaagtcttt ccattgtaac
      301 gctaagagct cctcactcct tacacctgta acaaaaataa aacgcatcaa acgtttataa
      361 aattcttcat aaaaatcccc gtgataaaca ctattatata ctttcttaaa ttcatcaact
      421 gtccaaaatt ctacttgagg acgctgagtt tttattttac ctatagtatc tacaggattt
      481 tcttcaataa tttcaagtac aattgccctt ttaaaaacta ctctcaactt attgaatatc
      541 tgtttaatat aatttggtga taatggttgc tcaatgccct tttttacaac atggattttc
      601 gataaaaact gttgaaattc ctgtatatga attggtcgaa tctttacaac ctgcatttct
      661 ccaaaaaaat ccaatgcacg ttgtaaaata tgatctgttt tcatatatgt tttttctaca
      721 gttcccaact tataccaagg aaaaaaatac tctcgataaa aatcatgaaa cgttattcct
      781 tgcagataaa ttccttgtga atcagaaaaa ctttgtttta actcttcgag tccccttcta
      841 gcttctcctt gagttttgaa gcctcttctg gtcacttgaa ttctttttcc aaagcgatct
      901 tgtcctaaag ttgcagaaaa aaaccaagtg cctctttttc tgtctttata aacattcgca
      961 attttcctca ttttactatt cctcccaaga aatatggaga acttcttcta aaattgaaat
     1021 aggaattctt tttatttttg aattacgata aaaaaagtat cctttctcaa ctaaaagttc
     1081 tttacattcc ttgaagattt tattagcata gtactgagaa aaacctagat taatcaaatc
     1141 ttttcgagta gctgttttca ttatagcacc ttccttactt tttttcattt atttctatca
     1201 gctgcttttg tcaaaagcac ataggaattt cacctctccc caagtttacg gcgagcctta
     1261 cggaag
//
LOCUS       DAYYME010000265         1258 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000265 DAYYME010000000
VERSION     DAYYME010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.271"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1034
                     /locus_tag="ACHBFW_002931"
     CDS             <1..1034
                     /locus_tag="ACHBFW_002931"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9319152.1"
                     /translation="IGIVKLYDFIILIRVRDKEMRQLHKEQIETAMAALKEKTIDHVY
                     FTACGGSQAVLMAGQYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTP
                     ETVEATKKAREFGAMTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLF
                     NILLVLDGSKKWETCLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGIN
                     YGEIYSTAACWFMEMQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRV
                     EDFTKKFSNKLMVLDQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDV
                     RRYMWQMKY"
     gene            1016..>1258
                     /locus_tag="ACHBFW_002932"
     CDS             1016..>1258
                     /locus_tag="ACHBFW_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFM9319153.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIIL"
BASE COUNT          445 a          166 c          242 g          405 t
ORIGIN      
        1 taataggtat tgtaaagtta tatgacttta taatacttat tagagtgagg gataaagaaa
       61 tgagacaact acacaaagaa cagattgaaa cagcaatggc agcgttaaag gaaaagacaa
      121 ttgatcatgt atattttaca gcatgtgggg gttcacaagc cgttttaatg gctggtcaat
      181 atctatttga taaagaatta actgtacctt gtcatgttta tactgctaac gaatttaact
      241 atgctatacc aaaaggatta tctgaaaatt ctttagttat cacttgttcc cattctggca
      301 caacaccaga aactgtagaa gcaacaaaaa aagcaagaga atttggagca atgactattt
      361 gtttatctaa tgaagaagga tctcctctat gggaagcagc tgaatatcct gttcactatg
      421 attggggtaa agatgttgat gctagtgatt taaataaagg aattctttat ggtttattat
      481 ttaatatttt acttgtttta gatggctcta aaaaatggga aacttgcttg aatgaatttg
      541 ataagttaaa tgatcttgtt attgctgaaa agagcaaatt tgaaataact gctaaagaat
      601 ggggtaaaaa ccaaaaacga gataaaatta tttacacaat tggtagcggt atcaattacg
      661 gagaaattta ctccacagca gcttgctggt tcatggaaat gcaatggatt aattctaatg
      721 ctattcattc tggggaattt ttccatggtc cttttgaaat cactgattat gatgtaccat
      781 tcatcttagt gaaaagtatt ggagatacaa gatttttaga tcaacgggta gaagacttca
      841 ctaaaaaatt cagtaataaa ttaatggttt tagaccaagc tgattttgac ttagaatcta
      901 tagatagtgt agcgaaagaa tatattgcag ctgtacttag tggcgttgtt attcgccaaa
      961 tggtagaagc gattgctttt gaaagaggcc attccttaga tgttagacgc tatatgtggc
     1021 aaatgaaata ttaattaata ggaggttgca aaagatggca aatcaattat ttcacattgt
     1081 gattacaaca cattctaccc tttgtgaggg ttatttaaag gcaacaaaat taattttaat
     1141 gaatgacgcc acaggtatta gtgctgttcc atttaaagaa aatactagtc cagatgattt
     1201 tgaggagagt atgaaagaaa taattagtca gcatcatttg caacctctaa ttatttta
//
LOCUS       DAYYME010000266         1255 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000266 DAYYME010000000
VERSION     DAYYME010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.272"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            320..1231
                     /locus_tag="ACHBFW_002933"
     CDS             320..1231
                     /locus_tag="ACHBFW_002933"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9319154.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT          495 a          191 c          251 g          318 t
ORIGIN      
        1 atcgaacata aaagtgtctg atttgaatat cccagcagcg gaagaagaaa aacaaaatcg
       61 ataacatttg ttctaagaat tagttacgtt aaatacggaa cgcaagatag acttgaaaaa
      121 cggtaacaaa agaagtagta tattaaggtg tgatgtgcta cttgacattt attctgctga
      181 gcgaactttc tggattgtgt agccttatat ctattcagga cgtatcgagc agattagtaa
      241 aggtgttctt gatgattttc atcttgaaca cctttgtttt tagcaattcg caaaaaacga
      301 aaagtaggag aattgataaa tgaaaaaaag atttttagca cttgccatcg tactaggcac
      361 tggcttattg tcaggctgta cgaatgccgg tgaaaaaaca gccgttagtt ataaaggcgg
      421 tacaatcagc gaacaagaag taatggatag cttgaaaaaa atgcaaggag cagattccgc
      481 tgtgcaacag ttgatcgttt atcaagtatt tgaagataaa tatggagatg acgtatcaac
      541 caaagaaatc gattctcaat atgatcaaac aaagaaacaa ttgggtgatt catttgattc
      601 acaattgaaa tctgcaggtt acacagaaca aacattcaaa gacagtatca aacaaagtct
      661 agcctttcaa gaaggtctaa agaaacatat caaattgacc gatgaagatt taaaaacggc
      721 ttgggaatct ttccatccag aagtggaagc tcaaattatc caagtagcaa gtgaagatga
      781 tgcaaaagat gtgaagaaag ctgctgataa gggagacgat ttctctaaat tagccaaaga
      841 caagtcaact gatactacga caaaagaaga tggcggaaaa gtgaaatttg attccactac
      901 tacaacggtt ccggcagaag tcaaagaagc agcctttaag ttgaaagatg gtcaagtttc
      961 tgatgtgatc acttctacca atgcttcaac ttatactacg gaatattatg tggttaaaat
     1021 ggtgaaaaac caaaacaaag gcaacgacat ggataaatac aaaaaagaat tgaaagaaat
     1081 tgcaacagac acaaaattaa gtgacagtac cttccagaat aaagtaattg gcgaagtatt
     1141 gaaagacgct aatgtaaaaa tcaaagacaa agactttgaa aatgttttga gtacattcac
     1201 ttcagattct agtacagcaa gtagtaaata agcggtataa ataaaaaata tgaac
//
LOCUS       DAYYME010000267         1252 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000267 DAYYME010000000
VERSION     DAYYME010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.273"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..905
                     /gene="scrK"
                     /locus_tag="ACHBFW_002934"
     CDS             33..905
                     /gene="scrK"
                     /locus_tag="ACHBFW_002934"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM9319155.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          376 a          230 c          302 g          344 t
ORIGIN      
        1 gatttcattc taaaataaag ggagaaatgt ctatgctata cggtgcaatt gaagcaggcg
       61 gcacaaaatt tgtgtgtgcc attggcgatg aagaaatgac gatcaaggaa agagtgagct
      121 ttccgacaac gacacctgag gaaacaatgc ctttagtcat tgacttcttc aaacaatacc
      181 aagcagattt agcagggatt gggattggct catttgggcc tatcgatatc catcgcgatt
      241 ccgccactta tgggtacatt acctcgaccc cgaaattggc atggcaaaat tttgatttca
      301 tcggtaccat gaaaaaagaa tttccgatcc cgatttcttg gacaacagat gtcaatgcgg
      361 ctgcctatgg tgagtatgtc ttcggcagtg gcaaaggatt gagcagtgtc gtttactata
      421 cgatcggtac gggtgttggc ggcggtgcct tacaagaagg gcgctttatc gaagggttta
      481 gtcatccaga aatggggcat atgctcgtcg tccctcatcc aaaagatgac tttgctggca
      541 gctgtccatt tcatggcaac tgtttagaag ggatggccgc tggacccgcc atcgaaaacc
      601 gcttagggaa aaaaggacaa gaggttgcag aagatgatcc ttattgggaa atcgaagcct
      661 cctatattgc tcaatgtgcc tacaatacaa ccttgatgtt ttctccagat gtgatcattt
      721 ttggcggtgg tgtgatgaaa caacgccata tgctgcaaaa cgtccacgac gcatttgcaa
      781 aattggtcaa tgggtatgtc gaaacacctg agttaagcaa atatatcgtg accccagcat
      841 tagaagacaa cgctggaacg ctcggttgtt tggcattggc tcgagaagcg gtcttacatt
      901 cataaacgaa aaaaggctct tcgacattta gtgttggtag agaaagatac aactgaaaat
      961 ttagtcataa ccagttaggc agagtgctta actggttttt gttttacttg gaagatcaag
     1021 ccttgaatga gataaattct tgctcgaaag ttatggaaat ttcgatagcc gtaggccact
     1081 tgtattgtgc aatagttttt tacaaaaaaa agggggtgcg gatatttttt tggacctttt
     1141 taacaaatag gttctgttgc aaagttttaa atctactatc agataaggta gaataataga
     1201 aaaagatagc aggaggaatg acgatgagtc attttaaagg aaagcaattt ca
//
LOCUS       DAYYME010000268         1249 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000268 DAYYME010000000
VERSION     DAYYME010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.274"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..963
                     /locus_tag="ACHBFW_002935"
     CDS             <1..963
                     /locus_tag="ACHBFW_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM9319156.1"
                     /translation="PINGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRN
                     EAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1152..1225
                     /locus_tag="ACHBFW_002936"
     tRNA            1152..1225
                     /locus_tag="ACHBFW_002936"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1186..1188,aa:Ile,seq:cat)
BASE COUNT          412 a          239 c          255 g          343 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagagcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
     1141 ggcaacaacc agtaccctta gctcagttgg tcagagcaga cggctcataa ccgtccggtc
     1201 gtaggttcga gtcctacagg gtacattaac gtagccattt gaatcgttc
//
LOCUS       DAYYME010000269         1246 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000269 DAYYME010000000
VERSION     DAYYME010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.275"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACHBFW_002937"
     CDS             200..742
                     /locus_tag="ACHBFW_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319157.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="ACHBFW_002938"
     CDS             898..>1246
                     /locus_tag="ACHBFW_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFM9319158.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAYYME010000270         1227 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000270 DAYYME010000000
VERSION     DAYYME010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.276"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(95..>1227)
                     /locus_tag="ACHBFW_002939"
     CDS             complement(95..>1227)
                     /locus_tag="ACHBFW_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM9319159.1"
                     /translation="LTSTTGIPILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPK
                     LICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLA
                     NAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTM
                     KTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITD
                     SVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLK
                     DRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWW
                     EQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT          380 a          249 c          224 g          374 t
ORIGIN      
        1 agaaaaacag ccaattggca tatttttatc tcatccaatt taaaaaaaca aaaagaaaag
       61 aaagaccaaa atggatcttt ctttccttaa atagttagga cttttgtgat tttaattctt
      121 tctcgatttg aagccgttgc actttcattg tagctgttcg cgggattgca tcaaaatcac
      181 ggatgatagg gacgttcaag cgaggaagtt catggacttg ctcccaccat cgattccaat
      241 ccatttcttt atcaggagct aaagcaataa tcggttgtgg agaatgattt ttatccctta
      301 caatcactac ttctgccaaa aaatctaacg aatctaataa aaagtcttca atagctaatg
      361 tactattgat cgtttcgatc aaatccactt gccgatcttt caaaaatagg tggccatgct
      421 tatccataaa accgtaatct ccgctatccc accaatcgcc ataaacattt tcctggaacc
      481 gtgcatcttc cttgtaataa gtcaaagccc gtcctttaga cagtaattgt atatgaccat
      541 ctgtgttttc aggtaggaca ttcccaactg aatctgtaat ccgagcgctt gtcatgtctt
      601 ccagtccaac gcccatatct cttgcatcac ttgttttcaa actctctagt gtatgagcct
      661 taagaatcat tggtccgcat tcactttggc catatacttg aagaaatacc gcatcctgtg
      721 ccatcgacgt tcgcaaaaat gtcttcattg tctggttgtt tatagcatca aaagtggagt
      781 gatagtatcg gatacccgaa aaagcttctg gatgctcctt agcagtaaaa cgccactgaa
      841 caaagttatt cggatgggtc tctagtgcaa ttggccggtg agcttccaac atatgaacaa
      901 cttttgaact ttgtgcgttt gctaaaggca tcataggaaa tcccatggac atcaaagaag
      961 agataccaat attgaatctg gaatgcactg gagagatgtg aaaagcgatt aatttctttt
     1021 ccgaaatttt tgtgaaaact gttttttgcc atttcgtccg ccatcccata gaatgagcag
     1081 aatgacaaat aagtttaggg ataccagttg taccagaagt atgggtcata taagaaattt
     1141 catttttgtc taaaaatgat tgagaaacag acaaagcagg ttgttccatt aaataaagaa
     1201 taggaatacc cgtggtgcta gttaatt
//
LOCUS       DAYYME010000271         1225 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000271 DAYYME010000000
VERSION     DAYYME010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.277"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..248
                     /locus_tag="ACHBFW_002940"
     CDS             <1..248
                     /locus_tag="ACHBFW_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM9319160.1"
                     /translation="TSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYL
                     ILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            253..1200
                     /locus_tag="ACHBFW_002941"
     CDS             253..1200
                     /locus_tag="ACHBFW_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM9319161.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          401 a          180 c          254 g          390 t
ORIGIN      
        1 acacatctag gcaacagaca cctgaagttt ttgaaatgaa tgcctcaatg tattctttta
       61 aacctgaatt tctaaaaaaa agtaattata tttttgatgg gcaatgtggg ataataaaaa
      121 tgagagatta tctaatttta gatattgata gtgaagaaga ttttaaatgg ttagaatttt
      181 tgtttcctaa atttttagaa gattcttcgg agcttcaaga tatctataat tatttatatc
      241 ggaggtaaaa ggatgagctg ttatcttatc accggtgggg ctggcttcat tggttctacc
      301 ttagccaatc atttaggaga agaacatgtg gtaatcgtcg tcgatgactt atcaatggga
      361 aaagtagaaa atcttgatat gacgaaaaat atcactttta ttgagggaga tgtcgctgag
      421 aaatccttga tggaagagat catgaaagct aataaatttg attatatctt tcatttagca
      481 gcagttgcta gtgtggcaga ttcagttgcc cgaccagtag aaacacatcg tgtgaatttt
      541 gaaagtgtat taatgatttt agaattgatc cgcaagtatc aacctgattt aaaacggatc
      601 gtgtttagtt cttcagcggc tgtttatggg gatgaaccga ctttaccaaa aaaagaagag
      661 tccgtgattc gcccattgac gccttatgcg gttgataaat tcgctgcaga acaatatgtg
      721 ctagattatt gtcatttata tgatgtaccc ggtagcgcag ttcgtttttt caatgtctat
      781 ggtccaaatc aaaatccgaa ttctccatac agtggtgtga tttctattct cgtagatcgc
      841 tataagaaac aaatcgtagg agaagcaact tcatttacat tatttggtga tggaagccag
      901 tctagagact ttgtttatat agatgatgta atccaagcat tacttttagt tgcgaatgag
      961 gaagctgcac tggggaaaca attcaatgtt ggaacgggta aagcaaccac acttttggaa
     1021 ttgattcatg caatcaatga aattctcggt gtagaattga cacttgaata caaagaagag
     1081 cgttcgggtg atatccatga ctctctagca gatattacta agatccagtc aattggctat
     1141 caaccgaaat atggtatttt aagtggaatg actaattatt tgaaaactga aatcaattaa
     1201 aacatactaa gataagatta gaggg
//
LOCUS       DAYYME010000272         1215 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000272 DAYYME010000000
VERSION     DAYYME010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.278"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACHBFW_002942"
     CDS             414..947
                     /locus_tag="ACHBFW_002942"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM9319162.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAYYME010000273         1170 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000273 DAYYME010000000
VERSION     DAYYME010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.279"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(199..1014)
                     /gene="thiD"
                     /locus_tag="ACHBFW_002943"
     CDS             complement(199..1014)
                     /gene="thiD"
                     /locus_tag="ACHBFW_002943"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFM9319163.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          310 a          255 c          193 g          412 t
ORIGIN      
        1 aaacatgaga agctgttttt tgaacttgtt ccttgtgact cggagctgga cggcaggctc
       61 acgacctctt tcttaaacgg tgttcaaaag cttgcatctg cggtaataag tccaaccaag
      121 ttaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcaactt
      181 tttcacaact tctttctttt actctaattc accagctgcc caatgattga tggggccata
      241 tttgtgccct acatttattt cttcacgaat tgcacggttt acatagtctt ttgccaatcg
      301 gatcgcttgt tcaactgttt ttcctttcgc taactcagct gcaatacatg cagatagcga
      361 atcgccagtt ccgttgatcc ggtcggtagg ataaaatggt gcatttaacc aaaatgattt
      421 tcctgattcg aacaatacgt agtcattgac tgtttcttgc tgcgcatttt tagcatgatg
      481 tcctttgatc ataacgtttt ttgcacccat atcttgcagt tcttttgctg cttgcacaaa
      541 atcttcttca gctgctaatg tccgtccagt cagcttttct gcttcataaa aattaggtgt
      601 cagtacttcg gccaaaggga caagcttttc tttcaagctt tgcaaagcag attcttctag
      661 taaaagattt ccatgtttcg ttacgattac gggatccacg actaatggtc caaaatcata
      721 tttttggtaa ttttttacaa ctgtctcaat caactttgaa tctgctagca tacctgtttt
      781 agctgcacgt atttgaaagt ccttcgcaat cagttcaaat tctttgtcga taaaatctgt
      841 tggcaatgtc acacttgctc cgattccgta agaattacct gcaacacatg cggtcatgac
      901 agaaacaccg taaacatttc ttgtaaagaa tgtatgcata tctgcttgca tccctgcgct
      961 tccatcgcta tctgatcctg cgatcgtcat tgcttgtaca tactgatctt ccatcaaatc
     1021 gattcctcca tttttataaa aagatttttc ataatactgt tactgtttct atcatatgtt
     1081 tttcaaagta tccctcagta aatataccac taccttttct tggtaatcaa atttatttga
     1141 aaaaacattt gcaataaatc agaaaatagt
//
LOCUS       DAYYME010000274         1153 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000274 DAYYME010000000
VERSION     DAYYME010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.280"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..627)
                     /locus_tag="ACHBFW_002944"
     CDS             complement(<1..627)
                     /locus_tag="ACHBFW_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9319164.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQ"
     gene            688..>1153
                     /locus_tag="ACHBFW_002945"
     CDS             688..>1153
                     /locus_tag="ACHBFW_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM9319165.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIE"
BASE COUNT          443 a          193 c          193 g          324 t
ORIGIN      
        1 ttgatacagt tgaagcgatt cctgatgttg tctttctttt acttggaaac tgatcaacat
       61 aatggaaaaa aacaaagaaa aaaaactgat gactatcgac aaaagagata gaagcagata
      121 attagatgag cggaagtgaa cacttcccaa taccaagtaa atcacgcccc agcttaataa
      181 aatacaaata cctccaaaat agtgttcagt tggataagta aaaagcaagt tgagaaatct
      241 cgtctgtttg tctatacttt ttactaatag atagaaaata aatgcgataa gtagtgcaaa
      301 aaaacttgct gttccaagat gtctctttcc taaataatcc atcagatcaa aaaccagaaa
      361 aaagctgagt ccagtgatag caaaaaaaag attactgatc aaacagctga taaacaggtt
      421 tttcgtagta aaataaaaaa gaaacatcaa taataagcca actaggtaag gaaagctcac
      481 tttagggata ggaagtaaaa gtgtcaccac catcaaagct atacaaccaa agctgttttt
      541 tactagcagt tgtttcgaaa agaataaata gatacctaaa tctaaaatca aagtaaaggt
      601 caaataaagt gtcactgttg cgaccatctt attcctccat tgttttgttt caaaatgtac
      661 acatattttt ttaataggag cctgcatatg aaaaagtatt ttatcataga agataaccag
      721 tttcacagcg aatggatcat cagagaattg gtcaatcatc atatcgagaa tatcgtgcag
      781 ctcacttcta tctctcaaat caatcagttg atccagttgg gcactattga taatcacgat
      841 gttttcttta tcgatatcga cttgaagcaa tcactcacag gtattgatgt cgccgaacaa
      901 atacgtaaaa aaaatgcaca tagtaatatt gtttttttca ccgcttatac gaattttgca
      961 gtagagatca tcaatcggaa aatccttccg attggatata tcagaaaaga caggaattta
     1021 tctgaacaaa ttcgtcaagt gctagaacag atcaattgga aagaacagca gatcgtaaat
     1081 gtcgataaaa tctttattga tcaaaaaggt ggaagtctcg tcctgtatcc tcgggaaatt
     1141 ttatatattg aaa
//
LOCUS       DAYYME010000275         1136 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000275 DAYYME010000000
VERSION     DAYYME010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.281"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            159..1004
                     /locus_tag="ACHBFW_002946"
     CDS             159..1004
                     /locus_tag="ACHBFW_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9319166.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT          442 a          138 c          192 g          364 t
ORIGIN      
        1 tatttataaa tcaaaaatac agagaatagt atataaatgt cctttcctat tcttaaaatg
       61 atgtttgtac ttacttaaat taggtataat tagtatatgt tttgtgagaa atttagactt
      121 acaataagca gtgaatttat aatcaacgta taggagacat ggatgaaatg aaactaattg
      181 gtatagattt agacggtact cttttgaatt ctgaccagag cattagtcaa aaaaatgcaa
      241 gaacaatgaa acatttaccg gaagactgtt ttccattcat ttgttctgga agagaagtag
      301 aagacattaa taatattctc aaaaaatctg gtttaaggat accagcagta gggttaaatg
      361 gttctgttgg atttgatgga gaaaaaaaga tatttgagtc ttccttcgat aaaaatgatg
      421 taaaaaaaat tgattctgtc ctatcgaaat ttccaaccaa aatatataca aatctcggaa
      481 gttatgaatc tcagaattat aaaaataaaa tgaaaaaaat ttttcacgag gtagggagtg
      541 aattttcaat caaagagcta aactatgagc ttgagtatga aaaatctatt tgttcaatcc
      601 cttataataa tattgaggaa attatagaaa aggatgctat cagcatttac aaatttttta
      661 tttttattcc taatcgtcaa ttgaaagcta atatatttaa ccatttgaaa aagatgctaa
      721 atgtgtctgt tactgaatcg gcacctgtaa atattgaaat cgtacctcaa aatgtatcta
      781 aaggtgtggt gtttgatcgt ttggaatcaa tctacaattt aaagaaacct ttgagaatcg
      841 cgatcggaga tagtttaaat gacttatcta tgtttgaaag tgcagatatt agttttgcaa
      901 tgggaaatag tcatcaagaa atcatgaaaa aagctgatta tgtcactgtt tcaaatgata
      961 aagacggagt atctcaagca tttgaaagaa ttcttaccat ataaactaat taaggtagta
     1021 tagaacatag aattctaaat acggaaaaat ggagcataaa ttccagtaca aatagtactt
     1081 cttcagtgtc aaacgaagcg aatataacat agatatttag gattgtgaca aaaatc
//
LOCUS       DAYYME010000276         1106 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000276 DAYYME010000000
VERSION     DAYYME010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.282"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..284)
                     /locus_tag="ACHBFW_002947"
                     /pseudo
     CDS             complement(60..284)
                     /locus_tag="ACHBFW_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(838..924)
                     /locus_tag="ACHBFW_002948"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="ACHBFW_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          387 a          187 c          159 g          373 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatat
//
LOCUS       DAYYME010000277         1091 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000277 DAYYME010000000
VERSION     DAYYME010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.283"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..697
                     /locus_tag="ACHBFW_002949"
     CDS             <1..697
                     /locus_tag="ACHBFW_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9319167.1"
                     /translation="HLETLNVIVEKVDYIFLEKPAFSESIDLTQYNYDYKNIYVAAPL
                     RYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAFKSMGGGVELDCIH
                     ELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIEIHLDYFGRVPTRK
                     FQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFFENVVTGKENWNDL
                     YHANEVLKIAEEK"
     gene            698..>1091
                     /locus_tag="ACHBFW_002950"
     CDS             698..>1091
                     /locus_tag="ACHBFW_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM9319168.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDSIIDLDLTSPLREVSNLEELIDEHL"
BASE COUNT          414 a          121 c          192 g          364 t
ORIGIN      
        1 gcatctggaa acactaaatg taattgttga aaaagttgat tacatatttt tagaaaaacc
       61 tgctttttct gagagtattg atcttactca atataattat gattacaaaa atatttatgt
      121 tgctgctcca ctgaggtata aaaatatcat ggaagttgtt cgtaatagta ttcaaagtaa
      181 aaaagtatat tctgtaagag ctatttgttc tacttatctt cctgattgga gaaaaggtac
      241 tgattataga aataattatt ctgcctttaa atctatgggg ggaggagtag aattagactg
      301 tatacatgaa ttggattata ttataaacct atttggtttc cctaaatcag taaaaaaaca
      361 gtttggtaaa tattcgaatc ttgaaattaa cagtaatgat ttagcaacat atatattgag
      421 atacgatgat aaaattatag aaattcattt agattatttt ggaagagttc ctacaagaaa
      481 atttcaattg tttactttag atgaatgtat caatgttgac ttattaaata ataaaataag
      541 ttttgaagaa aacattgtta aagaaatgcc tgaatctgct aatgatatgt atgagaagga
      601 gttgaaatat ttctttgaaa acgtagtaac aggcaaagaa aactggaatg atttatatca
      661 tgcaaacgaa gtattgaaaa tagctgagga gaaataaatg gaaattttat ttactatttg
      721 tggtagagct gggtctaagg ggtttaaaaa taaaaatttg aaatatttgg caggaaagcc
      781 cctagtccat ttcgctttag caatgataga tatctatcaa caaaaacatc ctgaacataa
      841 ttgcacgact tgtttgaata cggacagtaa tgagttagta gaattgacga ctaagttaaa
      901 tccgtctgtg aaaattgtag aacgtaaacc tgaattagct acagatactg tagggaaaat
      961 tgaagtgata cgtgattcat tcttacaact agaaagagaa gataatatat ttgattcaat
     1021 tatagattta gatttgactt cacctttacg tgaagtttct aatcttgaag aattaataga
     1081 tgaacatcta a
//
LOCUS       DAYYME010000278         1090 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000278 DAYYME010000000
VERSION     DAYYME010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.284"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            732..1046
                     /locus_tag="ACHBFW_002951"
     CDS             732..1046
                     /locus_tag="ACHBFW_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319169.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
BASE COUNT          429 a          141 c          184 g          336 t
ORIGIN      
        1 aaagccttag tcactatgac caaaactacg gacgaaacta tgaccaaaag taggtactaa
       61 attatgaccg aaaataggtc caaaatggat ttttttatga tatcaaaaat tatgaaatga
      121 ttttgaaaga aaaccagttg gcacagccaa cttagccacc ttcctgtagg aaggtgctat
      181 cagttttccc ttatgctctt ttctttcttt tttgtttcat atgtcaaatg ccattttgaa
      241 atgtaccatc ttaagaaatt tatccttaaa aacaattgcg ttattggata aaaagatgct
      301 acattatctt aaaaaaggaa tcaaatatga tgaatttagt ggctagcata tcgtgtgatt
      361 aagatacttt atagtgtaag ttgaaggaga aaaaatattt aaaatatact tcccatttca
      421 gtgggaagta aaattaatct tgttgtttct actagtaatg tatctttttg gaaactatgt
      481 aacgatgtaa acaagatttt tttcagaaga aaaaacgata aaagttgtga taaacgattt
      541 aaaaaatagg agaaaatata tcaagcgcat ggtatgaaac gcacatttaa aaagattcga
      601 ctaactaaaa aaataagttt acaaagcatt gttgtgtatt acaagtgatc tatacactga
      661 aaaatgatag agaaaatctg atacgtccgc aacaatggac aattgaaaat gaaagtgagg
      721 aaattggaag aatggctgat gaaaataaag ttgttccgct tgaaacacta gtgataaata
      781 aggaagagtt tgagcaactt tttcttgatt tttttgttca atatgacagg atttatgatt
      841 ttagttcgtc actagattta tttgaattag aggcagaaat aatagaaaac agtcgagagg
      901 agaatggtaa aatcatcatt tcagtagaaa aattcaatga attattctca gaaacatttg
      961 aaatagatac agattattat caaccacaag aagttttcca atcatttaga aatcagatta
     1021 ttcgctcagg taagaccgta aactgaaaaa caataaaaga aacgttgaaa gaataattat
     1081 tccttcaacg
//
LOCUS       DAYYME010000279         1079 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000279 DAYYME010000000
VERSION     DAYYME010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.285"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..571
                     /locus_tag="ACHBFW_002952"
     CDS             <1..571
                     /locus_tag="ACHBFW_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM9319170.1"
                     /translation="VINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNER
                     KEEFDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQ
                     GVRHESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVE
                     FPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            571..>1079
                     /locus_tag="ACHBFW_002953"
     CDS             571..>1079
                     /locus_tag="ACHBFW_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319171.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          381 a          139 c          241 g          318 t
ORIGIN      
        1 tgtcatcaat gctgtttcaa aaacagaagg agaaatgggt tcttattggc aatcaattga
       61 agatttaatt gctgatatga cagattttat taatgaaaat aaaggcgatt ttacggactg
      121 gatgaatgaa aggaaagaag aatttgatcg ctggagagag gaacaggaga atagtttcca
      181 agattggaga gaaggacaag aatcagatta cttgtcatgg tttgaatcaa tcaaggatat
      241 tttgaagtct atcgatccag gtggagtaat gttagccgaa ttaatggatg cacgtgtaga
      301 tcttcaaggt gtacgccatg aatcgatttc tgaacgtttc ctatccgatc taaattattt
      361 gtatcaaaaa atgaaagcag cacttttcac gattgaatac ggtgaaattg aagtgactga
      421 tattcttcaa gacgatctct tttcagataa tcacgaagtc gaaaaaattg aaacagtaga
      481 atttccaatt gaagaaggag ctttgattat tgcaactgtt gatgatccaa aacagaatgt
      541 tttcactctt gagaaagttg gggtgatttg atggttaaag taaagagaat gatggaaact
      601 gatgaatctg gtgttaaacg tcaatttatg ccaattacac atgtctctgc agttttaggc
      661 ttagaaaaaa tgatatcagg acaatcaaaa gttctttcgg ttaatggtaa atatggtgca
      721 gtcattttaa cgaaagctga tttaggatta gagaatgcaa ttactgaact accttatgca
      781 agcgagacaa gcgatggtat tcttactgct gaaatgtttc aaaagatcgt aaatggagaa
      841 ggtggaacat atattttgcc gattgctact cctgaacaat taggaggtat aaaagttggg
      901 gaactcttag aagttactga agaaggtgtt ttatctgcaa ctaagcaaac agacttcaat
      961 ttctctgaag aattaaaagg aaaattagaa tctctaaaaa tattaaaagc aggtgcaaat
     1021 atatcaattg cagaagatgg aacaattagt tctacaggtg gtagtgggac tggtggtgt
//
LOCUS       DAYYME010000280         1069 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000280 DAYYME010000000
VERSION     DAYYME010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.286"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACHBFW_002954"
     CDS             <1..969
                     /locus_tag="ACHBFW_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM9319172.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          185 c          214 g          302 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccat
//
LOCUS       DAYYME010000281         1047 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000281 DAYYME010000000
VERSION     DAYYME010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.287"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..493)
                     /locus_tag="ACHBFW_002955"
     CDS             complement(<1..493)
                     /locus_tag="ACHBFW_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HFM9319173.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEI"
     gene            complement(592..942)
                     /locus_tag="ACHBFW_002956"
     CDS             complement(592..942)
                     /locus_tag="ACHBFW_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFM9319174.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT          385 a          198 c          168 g          296 t
ORIGIN      
        1 taatctcctg gatgcccatg aaaatcaaaa taaagccgaa tatttggaat agaagcatca
       61 aacttttgaa tggattgaac aataagacga atccgccaat caacaacaga atactactat
      121 aaacactcca acttttagct tttgtattca tgctgaagaa caactggaac aagctgttga
      181 tgataacagc taatccaaga agaatcggca ggatagaaac gattgcaggt gcaaaagcat
      241 agacgatcag agctaagatc aggaaaaaaa gaccactgaa cagaccgata ctccaagaac
      301 ctgttttcct tttaaaacgg taagcctcaa aaagattcaa caacccaagc aaaacaagat
      361 aggcggtgat cagataaccg ataaaatgaa aaacagcaga agggtttagc acaatcgcaa
      421 tcccagaaat aatataaaca gcagctctta ataaggcgta tttttgaata ttttgtttaa
      481 tcgtctcaaa catataagaa aacctccttt gacttatttt aacgtgaact agtcatttta
      541 ccaaattaaa tgttacaaaa tgagcgaagg agaagaatat ctctggaaaa atcaacttct
      601 gaggatcttt tccatttttt tgccttttgc tagctcatcg atcattttat ctaaataacg
      661 aatttctttc atcaagttat cctcgatgtc ttctactcgt acaccacaaa caacacctgt
      721 aatcaaggaa cgtgaaggat tggggacagg ggcttccgca aaaaattgtt cgaaagtcac
      781 ttgtttttgt agttgatatt ctagttctgt ttgggaataa cccgtcagcc aacaaatgat
      841 ttcatctact tcctctttta tacgatcttt tctctccgct tttgctacat acataggata
      901 aacgcttgca aaacttgtcg taaaaatacg attttgcgcc ataatcagtc acctttcttt
      961 ttttcaaatt atttactact tatagtatat aagaaaaaga gaagaaaaag cgattattca
     1021 aaaaaacaaa aaggattgtg acaaaat
//
LOCUS       DAYYME010000282         1043 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000282 DAYYME010000000
VERSION     DAYYME010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.288"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1043
                     /locus_tag="ACHBFW_002957"
     CDS             <1..>1043
                     /locus_tag="ACHBFW_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319175.1"
                     /translation="YEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNM
                     VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPN
                     KDEITFTFKSSKG"
BASE COUNT          360 a          138 c          146 g          399 t
ORIGIN      
        1 gtatgaaatt accaaaaaac atgttactag ctcctttttc gctattatat atacaacatc
       61 ttcttttagg agtgtagaaa tatttcttcg aggggcaatg ggggaattac tcgcaatgtc
      121 aatacttcct cttattttac tcgggtttat aaagctttac gactcaaaaa aagaatcttg
      181 ggttatgttg gcgattagca tgactttatt aatttacact catgttcttt ctgtagctat
      241 gactatggta atgattataa ttgctttaat tattcaattt tatcggaaaa agaaaataga
      301 aatcttctta attattaaat taataaaagc tgctatagtt actttacttc tttcactagc
      361 gtttataggt cctatgattg agcagacatt gtatcaagat ctgaatagac cttatgtgga
      421 tatattacaa aaaagagcaa tttatctggg taaagagttt ttgattggaa gtatagattc
      481 agaattacta tcatttggta ttggcttaac tttgcttatt tgtttaatta tactaacatt
      541 taactttaaa aaattaggaa tcttatctaa aatcacttat ataatgggta taatatctat
      601 tctactttgt acaaaaatat ttccatggtt tgccttacaa aatactcctt taaatattat
      661 tcaagctcct tggcgtttta tgatattttc aacattgttc atatcttttt cagtatctct
      721 atttatttct ccacagttgg aaaaattttc ttatacaagg agaagaaatt ttatactatt
      781 attaatatta gctatttcaa ttttaaatta ttcatcagta gttaacatgg tgaaagaaat
      841 gaattttcaa ggaacttatt ctaatgaaca aatggaaaat aaacttgaag aatttgagaa
      901 ttttgattat gtacctatag gctataaaaa agatcaattg cttttgaaaa accatcaagt
      961 tacagaagat ggtcaagtta tttatagtag cttttctcca aataaagatg aaattacatt
     1021 tacgtttaaa tcttcaaaag gca
//
LOCUS       DAYYME010000283         1031 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000283 DAYYME010000000
VERSION     DAYYME010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.289"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..510
                     /locus_tag="ACHBFW_002958"
     CDS             <1..510
                     /locus_tag="ACHBFW_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319176.1"
                     /translation="MPKEGEMFNLDKIITWRENWSKNILGELEVGRVYTNKEISEKFK
                     CSKQGGMRRSHQTNTLVLFSDQTGSNVYKDKWLNGILQYTGMGLKGDQVLDKNQNKVL
                     ANSKSNFVKIHLFETFKPKEHTYLGEVYLAGQIYTVNEKDSSGNSRKVYKFPLALINQ
                     EQLIEDKDI"
     gene            491..937
                     /locus_tag="ACHBFW_002959"
     CDS             491..937
                     /locus_tag="ACHBFW_002959"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM9319177.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
BASE COUNT          416 a          124 c          187 g          304 t
ORIGIN      
        1 atgccaaaag agggagaaat gtttaatctt gataagatta ttacatggcg agaaaattgg
       61 tctaaaaaca tattaggtga gttagaagtt ggtagagtat atactaacaa agaaatttca
      121 gagaagttta aatgttccaa acagggtgga atgagaagat ctcatcaaac taatactctt
      181 gtactgttta gtgatcaaac aggaagtaat gtttataaag ataagtggtt aaatggtatt
      241 ttacaatata ccggtatggg gctaaaaggg gatcaagtat tggataagaa tcaaaataaa
      301 gttttagcca attcaaaaag taattttgta aagattcatt tatttgaaac atttaaacca
      361 aaagaacaca catatctggg agaagtatat ttagcaggac aaatatatac tgttaatgaa
      421 aaagatagtt cgggcaacag cagaaaagta tataaatttc cattagcttt aataaatcaa
      481 gagcaattga ttgaagataa agatatataa ccaggaagaa aatcaaacta gacatattcg
      541 taatttatct aatgctaaat tagaggaaga agcaagaaaa gttagtaatt ataataaaaa
      601 aatggctgca aaatatgtta acgaagagga gttttgcttc tcaaaaacaa aaatatataa
      661 acgtaatccc ttcgtatctg aatatgttaa acgacttgca aaaggtattt gtcaactttg
      721 ccaagaaaaa gggccattta taaaagacgg tgtaccttat ttacattgtc atcatataga
      781 atatttgtcg caaggtggaa aagatgtgat tgaaaactgt atcgcgttat gtcccaattg
      841 tcatgctaga attcatgaac ttgagttacc tgacgataaa gaaaaattgc tgaagatcgt
      901 tgaaaaaaga gaaaatagct tttctaaaat accgtaattt atttttatag gacaaataag
      961 ctaatgtgag ctatgccttc tatataagac taagttctat tataagtaac gaagtaattc
     1021 tcgttccttc c
//
LOCUS       DAYYME010000284         1027 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000284 DAYYME010000000
VERSION     DAYYME010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.290"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(182..886)
                     /locus_tag="ACHBFW_002960"
     CDS             complement(182..886)
                     /locus_tag="ACHBFW_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319178.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT          293 a          183 c          187 g          364 t
ORIGIN      
        1 atatcttcag gtagaagaga agtcgcctaa cattgtagga tcataggtga tttgttggac
       61 aggtcaagct gatatttgag cctgatgtta gaaggaattt ctttttactt gctgtagaaa
      121 ttattatatg actaaaaagg ctgggatttt aaatcccagc cttttatgac aaatagaatt
      181 tttatcgatc agtaatcgct gtcgctgcaa attgttcgat caaaggaatc aaagtcaatt
      241 tgactttgta tttccgttcg agtgcttttt cgataacgaa ctcattttca gtagtcatag
      301 aaatagttgc taatttacga ggttcatcag catttttttc attaataact acttcatgtt
      361 cattttgttc tgtgtaaaat ttaggatact cagcaacggc ttttgaaaaa tcagcaaaac
      421 ttaaagcggc attcgtatca gttgagcgtg tattttcagt tgtgtccgct tttggttttt
      481 ccgttttttc ttctggcaca ctgactgttt cttctgtaac tggtgtcgca acagcttgcg
      541 cagttggttc atcgtttttc aatcgttgat cattccagta agacacgatt tcaataagaa
      601 gattgtcacg gaatcgttcg aaaatcccgt tttctcctga acgaattctc tttaatacag
      661 tattgaagtt gtcatcgatt ggcacgttga tccgtgtatc ttcatttgtt ttcccagcta
      721 atccgatcgt taagatttga cggtttttgg gatcaagcat gacttgtaac ggcaaaccat
      781 aacgtcgcaa gctctcttct tctagagaac ggaataattg tcctcttaca ccatccaatg
      841 aaggacggaa ttgatttaat aatttttgtt tctgtttttt cttcatatgt tctctccttc
      901 gctcataccc tttcgattat aagaaaaaaa cagccaaatg aaaaggagta atgcaaaaaa
      961 acttgcgtat acgcaaatta caacagaaaa atttgcgtat tttcccgttt tttgaaagaa
     1021 aaataaa
//
LOCUS       DAYYME010000285         1025 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000285 DAYYME010000000
VERSION     DAYYME010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.291"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..720
                     /locus_tag="ACHBFW_002961"
     CDS             <1..720
                     /locus_tag="ACHBFW_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM9319179.1"
                     /translation="WNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNI
                     VTGIVNSAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWN
                     ALKDTIKGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMT
                     NLVKTPSVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIK
                     TELTRQMKRDAGAVGGTLYAT"
     gene            710..>1025
                     /locus_tag="ACHBFW_002962"
     CDS             710..>1025
                     /locus_tag="ACHBFW_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM9319180.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVV"
BASE COUNT          393 a          122 c          191 g          319 t
ORIGIN      
        1 tggaataata ttaaacagtt tttcaaagat acttggaaaa atattaaaga tacggcatac
       61 aatacgtgga tttctataaa aaataccatg attaatacat ggaataacat taaggactct
      121 ttctggaata ttgttactgg aattgttaat tctgctgaaa atgcatggac aaatctaaaa
      181 aatggtgttt caaatgctat taatcgggta aaagaaatct ttaattcgtt aagggaagtc
      241 aatttatttg aaattggcaa gaacatcatt gatggactta taaatggtgt aaaagaaaag
      301 tggaatgctt tgaaagatac tatcaaaggt attgcaggta atatcaaaaa ttggattaaa
      361 ggagctttag atattcattc tccatcaaga tggatgcgtg atatggttgg taaaaatatt
      421 gttcaaggta ttatcgttgg tattgataaa gagcaaagta agttagatca aaccatgaca
      481 aatttagtaa aaacaccgtc tgttcaacct gtagtaaatg gaataagtca cctaccaatt
      541 acacaaacaa agcaaaatat atctgctagt gaaacaaaag aaattcattt acatttaaat
      601 gtttatggag atttacctga ttcaatggtt cgaaagatta ctacaaaaat taaaacagaa
      661 ttgacaagac aaatgaaacg agatgctggt gcagtaggag gaacattata tgcaacttaa
      721 gagaggacaa ttttttatta atcaacatta ttcttctgaa tttaatgtgt atattcaaaa
      781 caggcctgct tctgtttcag ctagtcgagt aattgaattg agagagcgag aaggaaatga
      841 ttctatcatt attgataaag gctattacaa aaatgtgact agaaagattg agtgttacta
      901 taaagcacct tcaattgata tggtgcagga gtgggaagat cgaatcactg aatggttaga
      961 tatgtcgtct tatagtgatt ttattcttta ctatgatgaa caatatattt atcaagctgt
     1021 agtaa
//
LOCUS       DAYYME010000286         1022 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000286 DAYYME010000000
VERSION     DAYYME010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.292"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..461
                     /locus_tag="ACHBFW_002963"
     CDS             3..461
                     /locus_tag="ACHBFW_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFM9319181.1"
                     /translation="MLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFVSV
                     IYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFM
                     KVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            470..697
                     /locus_tag="ACHBFW_002964"
     CDS             470..697
                     /locus_tag="ACHBFW_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9319182.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          319 a          167 c          180 g          356 t
ORIGIN      
        1 cattgctatt aaaatttgtc gttttaggta taagtgccgt atttattttg ttcggcgtac
       61 ttttttatac tcagatcgct acaagcgaaa aaggattcaa ttttgattgg cagaccacat
      121 tatttgtttc tgtcatttac tttgcccttc tcctagggat cgttattgct tatttcttat
      181 tgaaactatt gaaaaaaatc gaagataacc aagctttttc cactcaaagc ttaaacatac
      241 taaaaaaaat cgaacgaacc attttgttta ttggtttcgc cttttttggc ttacttccta
      301 aatttatgaa agtagcggat ttagacgatg ctccaggttt gatgttggta ggaatcgctg
      361 tcgtattcat tccttttgca gtatacacat taacaagcgt gctggaaaaa ctgcttgaac
      421 atgcgatttt acttaagaca gatcatgatt tgacagtgtg aggtgcgaca tggcaattat
      481 cgttaattta gatgtagtat tagctcaaaa aaaaatgagt gtgactgaat tgagtgagaa
      541 agtgggcatt acgatggcaa acatctctat tctaaaaaat ggaaaagcaa aagccatccg
      601 tttttccact ctcgaaaaaa tctgccaagt tttggagtgt caaccaggtg atttgttgac
      661 ctataaaaat gactcgaata ctataccaga agaataaaat aaagtagaac aaacagtaaa
      721 taaaaaataa tctgaatgat agatgtgtga gacttttaga agttatttac gtctattatt
      781 cagatttttt atttcgattg aataccgttt tatctataaa taagatatgt actgactcat
      841 acaaaggggg ccttttcttc tccatgtact atcacttttc ctgatacaac tggtttcctg
      901 ttacgttgta gttggatctg cttttagtat aatactttat tcattccacc agactgatag
      961 cagtttgagc agtctaaata atcatgtagt attacaggcc attctgtttc ataccttgaa
     1021 gt
//
LOCUS       DAYYME010000287         1000 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000287 DAYYME010000000
VERSION     DAYYME010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.293"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            369..1000
                     /locus_tag="ACHBFW_002965"
                     /pseudo
     CDS             369..1000
                     /locus_tag="ACHBFW_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac
//
LOCUS       DAYYME010000288          990 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000288 DAYYME010000000
VERSION     DAYYME010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.294"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>990
                     /locus_tag="ACHBFW_002966"
     CDS             <1..>990
                     /locus_tag="ACHBFW_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM9319183.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQ"
BASE COUNT          373 a          122 c          192 g          303 t
ORIGIN      
        1 atgaaaccaa gaatatatag tcctactgaa acagatttct caacgaatgg cttaggaatt
       61 ttaaaggatt gtacaagatg tgaaatatat gaagtagcta atggaaaata tgaattagag
      121 ttggattatc ctttaggaac tagatttgat gaatattttg aaaatgacta tcaaataaaa
      181 gcaaagccaa acgatcaaga agagtatcat atctttttta ttgatgataa agatatagat
      241 acttttttaa atacagtaac tatttatgct cagagtcgta caaatcgact tggaagacgg
      301 gtagtcactc ttgtggaagt ggactctaaa actggtcaag aagcgatatc aattattgaa
      361 actaaaatgg ataaaaaatc tgacatacga ctttattctg atattacggc tgtttctagc
      421 acaatctttg aagcgagaaa tgttttaaat tgtattgctg gtgaacaagg atcattgctt
      481 caatattggg gcggagaaat taaacgtgaa ccatttaagc tttctttgtt aaagcgaaga
      541 ggtcgcaata atgttggaac gattcggtat ggaaaagata tgtctggttt gaaggtcaaa
      601 ttagattgga caggtataaa aacaagaatt attccatatg ctgatcctca aagtgaagta
      661 ggtacgacta atcgaattta tggttcgcca gtagatagtg cctatattaa taactatcct
      721 gatgtctata cagaacatgt tcagtttaca gaagaacaag gagtaaaaga cattaagagc
      781 ttaaataaaa tagctaaaaa ttactttaaa acgatcaatc ctggctgtga taagcctaaa
      841 gtttctatta cggttgaatt tgataagttg actgatagtg aagaagcgaa agaatttgcg
      901 aagattagaa attatggtct gtttgataca tttaaaatat atcataaaaa atataatatt
      961 tatcttgaat caaaggttag tggactacaa
//
LOCUS       DAYYME010000289          986 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000289 DAYYME010000000
VERSION     DAYYME010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.295"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>986
                     /locus_tag="ACHBFW_002967"
     CDS             <1..>986
                     /locus_tag="ACHBFW_002967"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM9319184.1"
                     /translation="LEPGDFIMSGAGTIGRISRVPKQIKPGVFNQALIRLRINKEITD
                     SEYFIQFIRADFMQRKLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFFKQL
                     DDTIALQQRKLDLLKETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSKKTG
                     KKNTKDLDFPAYSVSNKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARVNVG
                     SIAFNNLGMTVIVSSLYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREYLFY
                     ENFANIKFPFTRNKEEQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQKM"
BASE COUNT          375 a          159 c          189 g          263 t
ORIGIN      
        1 ttagaaccag gcgattttat catgagtggc gctggaacaa ttgggagaat ttcaagagta
       61 ccaaagcaaa taaaaccggg tgtattcaat caggctttga ttagacttag aataaataaa
      121 gaaatcacag attcagaata ttttattcaa tttattcgtg cagattttat gcaaagaaaa
      181 ctcacaggag ctaatccagg ttcagcaatt acaaatctcg taccaatgtc agaagtgaaa
      241 aaatggatag tacaatttcc aatattagaa gaacaaaaga aaattgggaa cttcttcaaa
      301 caactggacg acactatcgc tcttcaacaa cgtaagctag atttattaaa ggaaacgaaa
      361 aaaggattct tacaaaaaat gttccctaaa aacggagcaa aagtgccaga aattcggttt
      421 cctggattta cggaagattg ggaacagcgt aagttcaaag agtttagtaa gaagactgga
      481 aaaaagaaca cgaaagattt agacttccct gcctattcgg taagtaataa agcaggttta
      541 atttctcaaa cagaacagtt tgatggtagt aggttggatg atttagaaaa aacaaattat
      601 aaattcgttg aaccaaatga attcgcctat aatccagcaa gagtaaacgt aggatcaatt
      661 gcatttaaca atcttggtat gacggtcatt gttagctctc tttatgtagt tgtgaaaatg
      721 agcgaggact tagataatga gttcatactt cagtttatca agtcaccaac atttatcaaa
      781 gaggtcaagc gcaacactga aggaagcgta cgagaatact tattctatga gaatttcgct
      841 aatatcaagt ttccttttac taggaataaa gaggaacaac aaaaaatcgg cgctttcttc
      901 aaacaactcg acgacacgat cgctcttcat caacgtaagt tagatttgct gaaggaaacg
      961 aaaaaaggct tcttacaaaa gatgtt
//
LOCUS       DAYYME010000290          983 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000290 DAYYME010000000
VERSION     DAYYME010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.296"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..391
                     /locus_tag="ACHBFW_002968"
     CDS             152..391
                     /locus_tag="ACHBFW_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319185.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(753..>983)
                     /locus_tag="ACHBFW_002969"
     CDS             complement(753..>983)
                     /locus_tag="ACHBFW_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319186.1"
                     /translation="KYICQPIGLKQKVIGEVISKLENCVVICVDEYHDVDRNEILEKC
                     GKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
BASE COUNT          309 a          181 c          148 g          345 t
ORIGIN      
        1 atgtatctgg tttgaactgg gatatcaccc tctccatata tgtattacta agactaatag
       61 atactataaa agacttgata gttttgcatt gattttgcct aatactgagc ataggtgtat
      121 gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta tcagtttatc
      181 aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac cttcagatac
      241 gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt ctccggtaga
      301 aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac tgtcagcacg
      361 tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc aaataatgat
      421 gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta cgataacaag
      481 cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg atcggatgat
      541 ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag tataggtttt
      601 tatcgagttt ctccaccagg cctcgttaca gagacagatt ctctaggcga accttttgaa
      661 taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa aagcttggtc
      721 ttctcgcttt tgaaggcact ttatttaaat ctttatgaaa ctaaagtatg ttcatatttg
      781 ttgctatagt tatatagata ttctgttgtt tccaactcat aaacgtgagt atattttgcc
      841 actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata ttcgtctacg
      901 cagatcacaa cacaattttc taatttactg attacttctc caatcacttt ttgtttcaag
      961 ccaataggtt ggcaaatata ttt
//
LOCUS       DAYYME010000291          975 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000291 DAYYME010000000
VERSION     DAYYME010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.297"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..447
                     /locus_tag="ACHBFW_002970"
     CDS             37..447
                     /locus_tag="ACHBFW_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM9319187.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            461..817
                     /locus_tag="ACHBFW_002971"
     CDS             461..817
                     /locus_tag="ACHBFW_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM9319188.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          332 a          152 c          194 g          297 t
ORIGIN      
        1 tgtagaattg gattataatt aaaaaggagt gagaaaatga taaaagtata tacttcgcct
       61 agctgtactt cctgtagaaa agcaagagca tggttgcaag cgaatcaact tgaatttgaa
      121 gagaagaata tttttgccga accgttaaca gaaagtgaga tcaaaaagat cttggcagcg
      181 actgaaggag gagttcaaga tattatttcc actcggtcga aaatttacga aaagctgggc
      241 atagatttta acgaattaac tttaaagcaa atgatcgtct tattcaaaga atatccttca
      301 ttattgagac gacctatttt gattgatgaa aaaagaatac taatcggatt taatgaagat
      361 gagatccggt catttgtccc tcgtgaaatt cgatgtgttg ccagaaatct gatctattta
      421 caaaacaata acatcttaaa ttcataggga gggagccttc gtggaagtga aagtctatgg
      481 atcagcgcac tgtgtaacaa gtctgaaggc gaaacaatgg ttagaacacc atcaattgcc
      541 ttatcaattt gttgacttag aagaacgcga tttaagtaca gaagaagcag aagaattatg
      601 cagttttgaa gaagtgcaag cagaacagct atttgcgact tggtctgagg cttttcacaa
      661 cattgcgatt gacctttcct gtgtaggaaa aaaacaactg atttttttat gcacacaaca
      721 taccaatctc ctcagacgtc ctttgattat tatcgatgaa gttctattta ttggatacaa
      781 tgagcagttg atggaacagc ttatcttaaa taaatagact ttaactgttt taatcaagtc
      841 actgagcatt tcatacagtt gtaacagtcg aacacttagc atcaagcgtg ttatgtgttc
      901 tttttttgac tttatttttc tactatagag acatgctcaa tttatgtggt caaaattcaa
      961 atctaggtgg ggata
//
LOCUS       DAYYME010000292          965 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000292 DAYYME010000000
VERSION     DAYYME010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.298"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>965
                     /locus_tag="ACHBFW_002972"
     CDS             <1..>965
                     /locus_tag="ACHBFW_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319189.1"
                     /translation="DVFDLMNEFLGILSNAQILPTVNWQYISIKDNPKLFLQEFPEEL
                     IGETFSTLLQLLETNENDQLVQELANYNEYTGLSFVVDELSDKINILSSSWENRIRTK
                     VFLWWSEQYKSSPIVPKLLKDNSDNWLMKSDKVFLPTDTGVTVLPKELSSWVSLSILK
                     QTYVDELIYQIKTSSSWKNKWEETANAYKAERTGDKRILDAFSEKYLAIEFTEQSSSD
                     LIIGTINRQIDTVEKSISFINWFYDNYNEKLSAGSELSKVPFNLPNVAGDVQPSNKMY
                     IGKEYGNELGDKLFQDTDYTALKKIDCSSFAEERDFLSFILRCGV"
BASE COUNT          334 a          146 c          180 g          305 t
ORIGIN      
        1 tagatgtatt tgacttaatg aacgaattct tgggtatttt atctaatgcc caaatactac
       61 cgactgtcaa ttggcaatat atttctatca aagataatcc aaaacttttc cttcaagaat
      121 tccctgagga gctaataggt gagacatttt ccaccttgtt gcaattatta gaaacaaacg
      181 aaaatgacca actagtacaa gaacttgcta attataatga atacactggc ctgtcgtttg
      241 ttgtagatga actatctgat aaaatcaaca ttctttcatc ttcttgggaa aatagaataa
      301 ggacaaaagt gttcttatgg tggagtgagc aatataaaag ttctccgatt gtccctaagc
      361 tattaaagga caatagtgac aattggttga tgaaatcaga taaggtattc ttaccgaccg
      421 atacaggtgt tactgtgctt ccaaaagagc tgtcttcttg ggttagttta agcattttga
      481 aacaaacata tgttgatgag ttaatttatc agattaagac ttcatcaagt tggaaaaata
      541 aatgggaaga aacagctaat gcatacaaag ctgaacgtac aggagataaa cgtattctag
      601 atgcattcag tgaaaaatat cttgcaattg aattcactga gcaaagcagt tcagatttga
      661 tcattggaac gattaatcga caaattgaca ctgttgaaaa atctatcagt ttcattaatt
      721 ggttttatga taactacaat gaaaaactat cagctggaag tgagctgtct aaagttccat
      781 ttaatcttcc aaacgttgct ggtgacgttc aaccttccaa taaaatgtat attggaaaag
      841 agtatggcaa tgaattagga gataagcttt ttcaagatac agattacacg gcattgaaaa
      901 aaatagattg ctctagtttt gctgaggaga gagattttct gtcatttatt cttcgatgtg
      961 gtgtt
//
LOCUS       DAYYME010000293          954 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000293 DAYYME010000000
VERSION     DAYYME010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.299"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>954
                     /locus_tag="ACHBFW_002973"
     CDS             <1..>954
                     /locus_tag="ACHBFW_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9319190.1"
                     /translation="ILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLS
                     YLDMGVELQELVRIKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSF
                     KRSFMISVDLLEEIEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGT
                     SKLLEQRINNGFADMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLML
                     GADVRKVKQYLQLNEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAK
                     EGILEGAIKREKLSSTAMNDFAMPHIEVDFIKKPVICVYVNP"
BASE COUNT          373 a          127 c          164 g          290 t
ORIGIN      
        1 tattttaatc aaaatgattg aaaaagcatt ttcaattatt gtgccaatag atgaacaagc
       61 ttatgttgac attcacatta cttcgattga aaaaaatgct atgaatcacg acttagatca
      121 aacgaagctt tcctatttag atatgggtgt tgagctacaa gagttagtaa gaattaaatt
      181 aaatgaattt tatcctgatg aagaattgat caataatcta gtattacatc tcaatgcagc
      241 agttaaaaga ctcaaattgg gagtaaatat ctataaccct tatacggaca aaatcaaata
      301 ttcttttaaa aggtcattta tgataagtgt agatttatta gaggaaatcg aagagagatt
      361 ctgtatacat tttaatgaag atgaaatcgc ttacgtaaca ctccatattc aatcactttt
      421 ggatcgttac aaacctgaca aaacgaaagt gatattagtt tgtagtagcg gatatggaac
      481 atcaaaatta ttagagcaaa gaataaataa cggttttgca gatatggttg agatagaaga
      541 tgtactaagc atcaatgaac tacaagatag aaaagtcact gatgaacttg ttataagcac
      601 gattccgatt gaaaatacaa attttcctgt tattgttgtt agtcctttga tgttaggggc
      661 agatgtcaga aaagtaaaac agtatctaca gttaaatgaa caaacagaag atgcttttct
      721 aaatttatta gataaacgat tttgcttttt tacccaccaa ataaaggata atcaagtgaa
      781 cgttttaaat ataattacca aaaaattggt aaaagaaaat tacgcgaaag aagggatact
      841 agagggagca atcaaaagag aaaaactatc ttcaacagcg atgaatgatt ttgctatgcc
      901 gcatatcgaa gtagatttta taaaaaagcc agtaatctgt gtctacgtta atcc
//
LOCUS       DAYYME010000294          943 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000294 DAYYME010000000
VERSION     DAYYME010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.300"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACHBFW_002974"
     rRNA            <1..>943
                     /locus_tag="ACHBFW_002974"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAYYME010000295          930 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000295 DAYYME010000000
VERSION     DAYYME010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.301"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..252)
                     /locus_tag="ACHBFW_002975"
     CDS             complement(<1..252)
                     /locus_tag="ACHBFW_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319191.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSD"
     gene            complement(254..>930)
                     /locus_tag="ACHBFW_002976"
     CDS             complement(254..>930)
                     /locus_tag="ACHBFW_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFM9319192.1"
                     /translation="SIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDR
                     KVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETD
                     FYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIF
                     SLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYL
                     YLKKEN"
BASE COUNT          345 a          162 c          112 g          311 t
ORIGIN      
        1 atccgagaaa aattgattat ggttagttaa caagttaatt ttacgtttaa aggaagaacc
       61 gattttccgg tgattcacaa aaacaacatc attaaatagc cctaattctt ttatacgctc
      121 actatatctt ctagcgtttt caaattcaga ataaataaat aaatctcctt ttgattctcg
      181 gtaaaaattt tgataaacat ttaatatatt aatcaaatgt aaaggagtcc aagcaatgaa
      241 agctgtcttc atattaattc tcctttttta aatacaagta agaaatgctt cccaaataat
      301 accagaaaac aattgaaatg acatcgcgct tattgaaaag tacatgagcc tccaccattt
      361 catctataaa aatagatagt ataaaaatta aaacaatacc aagaaacgca taattcactt
      421 tacttctaaa aaatgttttg agtaaactaa agatatttaa cacatagaaa gcatagatac
      481 atataaaagc aattatacca cttgatacaa gtacttgaag aaatccatta tgcatgtttc
      541 catgtgctct ttttagttca ttcacatctg tagtatcaag atctactttt ttcaaaaatt
      601 ttgcatcgct attcccatag aaatcggtct ctccataacc aaataacaaa gttggtctta
      661 taactttaag cgcagctttc caaatcgtaa aacgaccagc tgaaatttcg gagttatcac
      721 tagaatgctg taatattact tctttttctg gaagtggagt tttatctgat tcatttgact
      781 ctataacttc tacttttcta tcaatcgata caggaattgt tgctaaaaaa tccttagcat
      841 aattagtacc aaaagaaaaa tataccaata gaactaataa tatgcttacc atccatttga
      901 tttttacagg tagtacttct ctaatgctct
//
LOCUS       DAYYME010000296          921 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000296 DAYYME010000000
VERSION     DAYYME010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.302"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="ACHBFW_002977"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="ACHBFW_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM9319193.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          267 a          192 c          138 g          324 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaattat acagaaacac tttattcaaa ataggaaaga
      841 tcaggaaagc catcaattgg aggaaaaaac tctgtttgtt catcatgata tagattatct
      901 tttgctttaa aaaaaaccaa t
//
LOCUS       DAYYME010000297          906 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000297 DAYYME010000000
VERSION     DAYYME010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.303"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>906
                     /locus_tag="ACHBFW_002978"
     CDS             <1..>906
                     /locus_tag="ACHBFW_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HFM9319194.1"
                     /translation="EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREV
                     QLVNRFIQIGDIYLTKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTAN
                     RIVERYIYDAIDMPLMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELD
                     AQKKFGLLNNVSTRMAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVC
                     SCASGLGTSFKLKRILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTL
                     NPHLEDVEFFSIEDMILGMNIRKFS"
BASE COUNT          330 a          115 c          155 g          306 t
ORIGIN      
        1 agaatttgac tattattatc ttcaaataac gtctatcttt ttgaattctt ggatttatca
       61 aatccaatta acatcggacg ataaattata ttccttcatc aatgatatta atagttggtg
      121 tagtcgagaa gttcaattag tcaatcgttt tattcaaata ggggatattt atttaacaaa
      181 aattcaaaaa aatgttttgg ttattttatt taagttaatg tgtagtaaag aatttcagca
      241 aaaacgttta gcactgattt tggctcatgg agatctaacg gcaacctgta tagcaagtac
      301 tgctaatagg atcgttgaga gatatatata cgacgccatt gatatgccac taatgacttc
      361 aagtagtgag attgcagaca aaattaatag atacttacaa gagtgtaccg gatatgaaga
      421 aattatttta cttgttgata tgggttcact ggagaaaatt tatctaaatt tggaactaga
      481 cgctcaaaaa aaattcggac tactaaacaa tgtttcaaca agaatggcta ttgatgttgc
      541 aaatcagata ttattagatt taccgcttaa acagattctt gaacaagtaa aagaaaatca
      601 aaatgtggat tatatttatg aatcatcaaa tattaagaag gatatgattg tctgttcttg
      661 tgcaagcgga ttaggaactt cgtttaaatt aaaaaggata cttgaagata gctttccaag
      721 ggatactgat cttgtcatag aaacgtgtga ctattactct ttagtgaatg aaaactattt
      781 atcggagctt cgtaaagaga acaatattct ttttattgtt ggaacactaa atccacatct
      841 agaagatgta gaattctttt caattgaaga tatgatttta ggaatgaata taagaaaatt
      901 ctccaa
//
LOCUS       DAYYME010000298          904 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000298 DAYYME010000000
VERSION     DAYYME010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.304"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            27..428
                     /locus_tag="ACHBFW_002979"
     CDS             27..428
                     /locus_tag="ACHBFW_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9319195.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            422..667
                     /locus_tag="ACHBFW_002980"
     CDS             422..667
                     /locus_tag="ACHBFW_002980"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9319196.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
BASE COUNT          343 a          125 c          154 g          282 t
ORIGIN      
        1 aaagggggaa aaaggagcat attatcatgc aacaacatta caatgacata tctatacaac
       61 accaatttga tgcctactgt aaaaaagtat tacgaaacga agctaagtct attcgaaaac
      121 gaaacgctaa cataaaggat aatgaggaac cattagcaga ttcaaatgaa ggcaggtatt
      181 cccaaactca ttttgatgaa caagatgtct atttcttatt tggaatggaa atattaattt
      241 cagatcaaaa gttatcagca gcaattgatc agttatcgga gataagaaga aagattatcc
      301 tactttatta ttttgcaggt tttaatgatg ctgaaatcgg taaaattctc aatatgagca
      361 caagtggtat ctggtatcaa cgaaagaagg cagttgaaca attgaaaatg gggtatgacc
      421 tatggtagat aaatctaaaa agcggcagta cctattagtt ccactatcca ttatcttatc
      481 tgctctagaa ggagatccaa tttcgatcaa gatgatactc aaacattatc attcttatat
      541 ggctaagtta tgtttgacaa atggatttaa cgaggtaggt caattcgtta cctatgtaga
      601 tgaatatatg ataagtcagc tagaaattaa attaatagaa gcgatcttaa aatttaaaat
      661 taactaatta aaaaaatccc ctttccactt tttctgttta aaagaatctt ctaatagtta
      721 aggaattagt agattctttt aggtagaaaa cctattgttc attgaaaatt aaatagattc
      781 tatcagtacg ttaacacttt aaggagcata ttttagttgc gccatgattt cttgctgtaa
      841 agaaggagcg ataaatattc taaaataatt tttatagtca ataaaaagcg atgatttaaa
      901 gttg
//
LOCUS       DAYYME010000299          890 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000299 DAYYME010000000
VERSION     DAYYME010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.306"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..888
                     /locus_tag="ACHBFW_002981"
     CDS             <1..888
                     /locus_tag="ACHBFW_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFM9319197.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DAYYME010000300          883 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000300 DAYYME010000000
VERSION     DAYYME010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.307"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..547
                     /locus_tag="ACHBFW_002982"
     CDS             140..547
                     /locus_tag="ACHBFW_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319198.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            561..>883
                     /locus_tag="ACHBFW_002983"
     CDS             561..>883
                     /locus_tag="ACHBFW_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319199.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDY"
BASE COUNT          338 a          116 c          148 g          281 t
ORIGIN      
        1 tacaaatata cttataaata tgcttacaaa attagctaat atttccgttg acaaagcttt
       61 gttactattg ttaaactaaa ttgactagca aaagtgaaac ataaatttat taaataaatg
      121 taaaaattag gagaaatcaa tgtcttatag agtacaatta ataatttcag aagatgtaga
      181 aagtcagcaa tttggcacta atgttattaa taaagtaatt aatccagctc tttctattaa
      241 tgcgccttta attcctactg ctttatcatt ttctgtaaca gctgtggtgt ctgagataga
      301 ggatacagaa aagataaaaa tagtagaaat tgaagtttta aacaagaatg aaaaacaaat
      361 tttttctacg ggtgaagtat cggtaagctt gcctcctcaa gttaacgata taaactttaa
      421 tataaacgcc agaaatgtct tggttgaaga agcaggagag cattatgctg ttgttaaatt
      481 taatggaact gagattggaa ggcaaatctt tgatatcaag gtcaacaaac cagtggaaaa
      541 aaattaagga gacgatacgg atgcttgata tagtaccatc aaatacacat cggagtggta
      601 gcttaataaa ttttgctgcg atttttgctt gcgcatcggt aattctagca tctccagttg
      661 ttgtggatgc aagttctact ccgaaaacta aaaatgataa tcaacttgta attactacaa
      721 aaaatgagat taacacaatt tcaagtaatg atggcaaaat ttttgatgtg ataaatacgg
      781 ttttaaaaag aaaagctgaa atcaatataa aatatgatga agatttaaac ttgtatttct
      841 ttgttatcaa aacaacatct gaattgtttt cctcagatta cga
//
LOCUS       DAYYME010000301          869 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000301 DAYYME010000000
VERSION     DAYYME010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.308"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..770
                     /locus_tag="ACHBFW_002984"
     CDS             78..770
                     /locus_tag="ACHBFW_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM9319200.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          382 a          136 c          153 g          198 t
ORIGIN      
        1 atttaaaaac gcttgacatt gtgtttgaga tcacttactt ttagcatgta aaagtattgg
       61 attaaggagc tatgcaaatg aaaaagaaat ttattactgg tgggattgtc gttgcatttc
      121 tatgtctaat tggctacaca ggcacgcggt acgcaaatga aattattttg tggggtggag
      181 agacagatat tgatacaatc aatgagaatc tgacacagct tgatcataca ttgacagata
      241 aagagcaagc gattacagat cttaccgctc gactagcaca aaaagaacag acattgcaag
      301 attcaaaggc agatgtccaa gaataccaaa caaaagtaaa tgaattagaa aaccaaaaaa
      361 atcaatcggc agcagaaaaa caaaacttag aaagtcaatt agtcagcaag gatgcacaac
      421 tacaaagcaa acaaaatgaa atcaatgtca aactagatga gatcaaccaa aaaaatgatg
      481 aaatcaatca tctgaatgca gattggacag caagactaca agaagcacaa gcaaaacttt
      541 cagaattaca aaatactgta aatggtttga atcaacaaat cgcaacactg acacaagaaa
      601 aaacaaatgt agaaactcag ttaacagata cacaaaagaa attagacgaa gcaactgctg
      661 aaaactcaag ctggaaacaa tatattcaaa agttagaaaa agcaaaagat gaagtggaag
      721 atacagctga taaatctcag caaattgttg aagaacattt gcgtaaataa tctttcttaa
      781 caagtaaacc taaattagta aagaagcaac agtaatctat actaagtagt tcagttttgg
      841 agtacaaaat gacaaaatgt acttcaaaa
//
LOCUS       DAYYME010000302          869 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000302 DAYYME010000000
VERSION     DAYYME010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.309"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            225..857
                     /locus_tag="ACHBFW_002985"
     CDS             225..857
                     /locus_tag="ACHBFW_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM9319201.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT          309 a          120 c          134 g          306 t
ORIGIN      
        1 tttttatttt aaaaacattc taaattaaat cagtaaaaat ttttattcta agatttttat
       61 tagaattacg cctaatttgt gtctctcagg gcaattctaa gaacgtttca agagatagcg
      121 acactatcgt tatcgaaaaa ggcgaaaacc tatctcattt tctgctaatg taagttggtt
      181 tagcttttgt taatatgact ttatgaaaat ggaggtaatt aaatatgatt tttcctaaag
      241 ttaatgaagc aagcttgaat aaaggatcct tttttcatat ctatgaaaac gaagattttg
      301 aactgtataa tatcctttca caaaaagagg tcatcgaaaa ccaaataatc tgccatccaa
      361 ttatttacaa atctattttc aagtatgcat tttttgcttc caatgatttt caaagaatgg
      421 agattaccga ctgtatattc gaaaactgtg atttttcagg ctgtatattg acaaaaagca
      481 tgttgcatcg cgtgaaattt gtcaattgta aattaacagg tactaaattg atggaaagtt
      541 atattgggaa cacgttattc gagaattgta aaatggacta tgtaaattta tctggttcaa
      601 acataaaaga aagtaccttt gagcactctg tcttatcaag cgcagatttt gtagactgtt
      661 ccctcactaa aataatgttt tctactaatg atttagaaaa tgctagcttt tttgatactg
      721 atttaaaaaa tatcgattta agtagcaact cctttgaaat gattgaagta cgctcggaac
      781 atctaaaaaa ttgtacgctt aatcagttgc aagcacttgg ttttgctaga aaatttttac
      841 aaataaaagt agtataatca aaatgaata
//
LOCUS       DAYYME010000303          862 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000303 DAYYME010000000
VERSION     DAYYME010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.310"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..>862
                     /locus_tag="ACHBFW_002986"
     CDS             141..>862
                     /locus_tag="ACHBFW_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9319202.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIET"
BASE COUNT          337 a          120 c          153 g          252 t
ORIGIN      
        1 tgatataaat attgcaggct tttttattga tttgaaataa tcaagaatat tagaaacttc
       61 tacattaatc aagaactttc ttctgtatga aaatagagat aataattgta cacttaagct
      121 gtattcagga ggtgaagata atgggcatcg aatttagcga acggcaaatg aaaatactat
      181 actttttgat tgaaaatgag cacacaactt acaaagaatt atcagaacaa ttatatgtat
      241 ctacgaaaac aattagcaaa gatattttaa ttttgaaaga aataatccaa aaaatgccag
      301 atgtacaaat gttggttcaa cctaaaaaag gcgtttcgct tattggaaaa aaagaaacac
      361 ttcgaaattt attatccagt cataaaatca atatcgcaga ctctccggag gaccgtctaa
      421 cttatgtgtt tgctaaatta ttgaatacta atgattctct aaagattcaa aatcttgctg
      481 acgaattata cgtgagcagg tcgacggtag aaagcacaat gaaagatgtt agaaaaagac
      541 taaacaagca tgggatgtcc ttagtttcag atcgaaatgg cgtgcgcatc aaagctgatg
      601 aaaaaagtaa aagaaggttg atgtcagaag ttgtgaacta ttattggggt ggctttacag
      661 catctaacag taaaacagat ctcttagact tgaatatcaa tttcattggt gaagcgaata
      721 atttcttaaa taaggacata ttgaaaaaag tagccgaatt gcttaatcga tacatgaata
      781 agacacaatt gaaatttaca gattatgaat ttcaaacttt agctatccac cttgctattg
      841 cgatagaaag gattgaaaca ag
//
LOCUS       DAYYME010000304          838 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000304 DAYYME010000000
VERSION     DAYYME010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.311"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..731)
                     /locus_tag="ACHBFW_002987"
     CDS             complement(<1..731)
                     /locus_tag="ACHBFW_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM9319203.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGIS"
BASE COUNT          256 a          154 c          113 g          315 t
ORIGIN      
        1 gaaattcctt tttctttatt gaaagacttt aattcttctc ttgacagtcc ccaatggttt
       61 acagcttcca tatgaacggc aataattgtg gaatctggta ttgctttatg caattcatat
      121 aaatcgtctt tccccataat aagagattct ccaactaaaa aatgattatc tccagcattt
      181 gctactatga tttctggatt atactgatca atagtttgtt taacagcttc ataccaaact
      241 gtatctcctg taaagtatag tgtcttctca gtcgaatgtt tgaaaacaat accgcaaaca
      301 ttacctacat aattcaagat ttctcctctg ccatgtttgc cttttgtttt aaccaattgt
      361 atagattcaa aaactgtgtc ttctcgtagt acctccacat ttttaaaacc atatagtcga
      421 atttcattcg catcattttc atcttgcaca aataatttta tttctttagg taggacctct
      481 ttcgctatat cgtcccaatg atctagatgt aaatgtgtta caataacagc atcaataccg
      541 ttgataatat cttcgactgg cattggcaaa tcaactagag gattcatttg atcttttctt
      601 ggtgatggaa aaggaccttt cgaaataaat ggtggaagtg ttcctttttc cgctaacatt
      661 ggatcaatta aaaatttttt acctccatag tctaagatag ctgttgcatt acgaatgtga
      721 tgtatattca taaacgttgt ctcctttttg ttgatatgaa atatttttag tataattcaa
      781 ttccatacaa taaataaacc gaacaaaaaa agtcttttac tatttttact taatttat
//
LOCUS       DAYYME010000305          833 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000305 DAYYME010000000
VERSION     DAYYME010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.312"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>833)
                     /locus_tag="ACHBFW_002988"
     CDS             complement(<1..>833)
                     /locus_tag="ACHBFW_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9319204.1"
                     /translation="NLVKKTLTYSIDTHLHLSAAPYFRLLINESANLYAEFAHKSIEL
                     DNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFSIDQEITSIITF
                     LKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCLQKIQLSSLPRH
                     YQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLLEQLAQIFFISP
                     ASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGFHSIHHFSSAFK
                     K"
BASE COUNT          285 a           96 c          147 g          305 t
ORIGIN      
        1 attttttaaa agctgaagaa aaatgatgga tcgaatgaaa tcctaatata tctgcaattt
       61 ctgtataggt taattcattt tctagcacga gacgttttgc tttttccatt tttagttctt
      121 taaaatagct aataacactt tttccattca cttttctaaa atgttttttt aagctggctg
      181 gactgataaa aaaaatttgg gctaattgtt caagcaaaaa attttctgaa agatgttctt
      241 ctaaatatcg tatgattgga tcaactaatt catctgaata aagaatatgc ttttttttag
      301 gaatgacttt ctcatttgaa ggtattgtca ataaagcaag ttcctgttga taatgccttg
      361 gtaaggaaga caattgaatt ttttgcagac aataagtaac taataaggaa agcaaatgaa
      421 tgctcatctg ttggcatagt atgaaaaaag aattgatttc ctgatcatag gaaatagctg
      481 gcttactatg ttttagataa tcaattttga actgttcatt tttcagaaaa gtaatgatag
      541 atgttatttc ttgatctata gaaaaagatt gtcccgtaat atgtgctaat aattgctcat
      601 caaaataaaa ctggataatg ataaaagttt gtgtttcctt ttgagtagaa aagatttgga
      661 cttcttgctg tttatcaatg aataaaaggg aaggattatc tagttctata gatttatgtg
      721 caaattcagc atataaatta gcgctttcat ttattagtag tcgaaaatag ggagcagcag
      781 ataaatgaag atgggtatct atggaatagg ttagtgtttt tttgacaaga ttg
//
LOCUS       DAYYME010000306          816 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000306 DAYYME010000000
VERSION     DAYYME010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.313"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>816
                     /locus_tag="ACHBFW_002989"
     CDS             <1..>816
                     /locus_tag="ACHBFW_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319205.1"
                     /translation="EAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQ
                     GSAGGYVVLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLD
                     SIFNANFIQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNS
                     KGSIGTIGTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNG
                     KAIYFGDLDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT          299 a          101 c          172 g          244 t
ORIGIN      
        1 agaagcagga gacattcaag ttgcttttta tcagcaacaa gctgtaacta ttcaggataa
       61 attaaaagat tatgcaacga ataattatat gagtgatttt aatgattatg tttcttcaat
      121 gattacaggt caaggcagtg caggaggata tgtagtttta tggccaaaag aaaaaccttc
      181 caacatcttt attatggata atccagattt agaaaaggca agagaagtac taagaatgaa
      241 taaaaatggg attgcttttt ctaagaaagg atggcggggg ccttttaatt cagcttggac
      301 attagatagt atatttaatg ccaattttat acaaactgga ttaatcaaag cagatatctt
      361 tcaaaattct tttaacaaga ctggagatgt attgaaatta gttaatggac tactccaaat
      421 ttggaacaac aaaaagaaaa taatggagtt aactaaaaaa ggaatggaat tttggaattc
      481 taagggttca attggaacga ttggaacaac tgattctgtt ggtaatcctt ttcctggggc
      541 ttctactcca acccctattg aagataattc tttagttatt cgtacaaatg gagacggcaa
      601 atatattttg atttctccta aagtaggaaa aggattagtt ttattaggaa atggtaaagc
      661 aatttatttt ggagacttag atgtacaagg aaaacttaca gttaaaggaa aagaaataat
      721 tcctggacaa ggtggtggtc catcgggagg tggagaatct actggtgggt atcctagtga
      781 attaaaaaca gatgcagaaa agagagcttg gagaat
//
LOCUS       DAYYME010000307          802 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000307 DAYYME010000000
VERSION     DAYYME010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.314"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..613)
                     /locus_tag="ACHBFW_002990"
     CDS             complement(<1..613)
                     /locus_tag="ACHBFW_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFM9319206.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          240 a          156 c          147 g          259 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tgactggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttccatga gaattgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ccttccacaa tcatggcaga
      361 gaaaacgggt acgttttaat tctaaaatag ttaacttttc tttgactttc aatatctgag
      421 tgcgtgtcgt ataggaaccg ttgcggataa tacgaaccga tcgacagtgt tcacaatagg
      481 aaggagagtc atccaatttt cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag
      601 tatgattatc cattgagaga ctcctttggt aggatgacgt tttggtcgac taaattttac
      661 caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc
      721 gattttccac caacacaaaa tattatagaa gcaaaatcaa ttctacctta gatgttctta
      781 tatcactcta tcagtcctat tt
//
LOCUS       DAYYME010000308          790 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000308 DAYYME010000000
VERSION     DAYYME010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.315"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            62..733
                     /locus_tag="ACHBFW_002991"
     CDS             62..733
                     /locus_tag="ACHBFW_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9319207.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT          314 a          117 c          124 g          235 t
ORIGIN      
        1 tattatatgg catgaacaga attaaaatat cagttaaaat aaaagttaag gagcattgtg
       61 tatgacaatt aaaactagtc tccaaaatca agcttaccaa tctattcgcc gacaaattat
      121 ctatgctgac ttagaacctg gaaaaaaagt atcagaaaaa aaattagaag aaacactttc
      181 tattggtcgt acaccaatac gtgaagcact gattcaactc cgacaacaag gtcttgttga
      241 tacaattcct caaagcggaa attatatctc attaattgat ttagattcgg ccaaaaatgc
      301 tcgatttgtc agagaacatt tggaacgaca aattatgtta gagtgttgtg ccaaaatgaa
      361 taaccaaaaa aaacggattt tagaaactat aatttcagaa caagaaaaag ctgttagtca
      421 aaaaaatgct cgtagttttt ttcataatga taatttattc cataaagctt gttttgaaat
      481 tgcagagcga gaagaagtat ggcaatggtt agaaaataac aatacacact tagaacgatt
      541 tcgatggcta cgagtaaaaa cggaaggatt aaagtgggaa aaaattatgg aacaacatta
      601 tcaactattt gaggcattaa caaaacaaga tccagaagaa gctaactttc taacatcgtt
      661 gcatcttcat ttaatgctta atgagcaaaa tattgtttta gctactcatc caaaatattt
      721 caaaaatata taacttttaa ctctttttaa attaaatggt tgttctgatc agaaaaatat
      781 cgcttgtttt
//
LOCUS       DAYYME010000309          768 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000309 DAYYME010000000
VERSION     DAYYME010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.316"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..387
                     /locus_tag="ACHBFW_002992"
     CDS             <1..387
                     /locus_tag="ACHBFW_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9319208.1"
                     /translation="DRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLI
                     ENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDT
                     YNQKFLNRSHKGFQQVTDTLVSMFTE"
     gene            468..>768
                     /locus_tag="ACHBFW_002993"
     CDS             468..>768
                     /locus_tag="ACHBFW_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319209.1"
                     /translation="MKNLTQNLLKKDNSSILKIELKKENNKSMKISKKAFLLVVLILL
                     STLYSVNFMRNAQEIYTTGDLSFHLSRIKGLSSIFEGPINYTTFNNYGDGLNYFYP"
BASE COUNT          295 a          125 c          107 g          241 t
ORIGIN      
        1 gaccgaaaag aaatctgtga tgactttaag gctgtttatc aagctaactc aaaagaagaa
       61 gcgaatacct tcttatccgg catgattgag aaatggaaga aaaactatcc taaagtgacg
      121 cagtcactca tagaaaacca agacttatta actttttatg attttccacc tagcattcgt
      181 agaaccattt actcaaccaa tctaatcgag tctttcaata agcaaattaa aagatacagc
      241 cgtagaaaag agcagtttca aaatgaagaa tcactagaac gctttctagt cagcattttt
      301 gatacataca atcaaaaatt tctaaacaga agccataaag gttttcaaca ggtaaccgat
      361 acattagttt caatgtttac tgagtaacta attattttgc aggaggacaa tttatttaca
      421 caaaattatt gacgctccct tcttttctag tatatcatac tagtgctatg aaaaatttaa
      481 cgcaaaactt attaaaaaaa gacaattcta gtatactgaa aatagaatta aaaaaggaga
      541 acaacaaatc tatgaaaata agtaaaaaag catttctttt agtagttctt atcttactta
      601 gtactttata ttcagtaaat tttatgagaa atgctcaaga aatttataca acaggtgatt
      661 tatcttttca tctttcacga atcaaagggt tatcttctat ttttgaaggg ccaatcaact
      721 atacaacatt taacaattat ggagatggac tgaactattt ttatcctt
//
LOCUS       DAYYME010000310          754 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000310 DAYYME010000000
VERSION     DAYYME010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.317"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..541
                     /locus_tag="ACHBFW_002994"
     CDS             <1..541
                     /locus_tag="ACHBFW_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM9319210.1"
                     /translation="TDLALNKLKELKIDESKYFVEKDSNAFEESLESVSGIKYIIKVM
                     TYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQFIFELIFISIPSIIS
                     SLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQSYLILISIIVLSVV
                     FASSLMLIKKPKEILSKIS"
     gene            551..>754
                     /locus_tag="ACHBFW_002995"
     CDS             551..>754
                     /locus_tag="ACHBFW_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9319211.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNED"
BASE COUNT          289 a           74 c          128 g          263 t
ORIGIN      
        1 tacagactta gccttaaaca aattgaaaga gcttaaaatt gatgagtcaa agtattttgt
       61 tgaaaaagat tctaatgcgt ttgaagagtc tttagagtca gtgagtggaa taaaatatat
      121 aataaaagta atgacttatt ctattatgtt aggtgggatg gttgttcttt cattaatctt
      181 gattctatgg ttaagggaaa gaatttatga aataggtata ttcttatcta ttggaacatc
      241 taagatacaa attataatgc aatttatatt cgagttaata ttcatatcga taccaagtat
      301 aatatcttcc ttatttttag gaaatgtatt actaaaagta attgtagatg gattcattaa
      361 ctcagaggat tcaatgattt ccggtggaag tttaataaat aatagtagtt ttatgtcaaa
      421 tataacaaca cttggacaaa gttatttaat attaataagt attattgttt tatcagttgt
      481 atttgcttct tcattaatgt taatcaagaa gcctaaagaa atcttatcaa aaataagtta
      541 ggagaaaata atggatatat tagaaataaa gaatgtagca tatagttatg caaattctaa
      601 agaaaaagtt ttgtcaggag taaatcaaaa atttgaactt gggaagtttt atgcgatagt
      661 aggaaagtca ggaacaggaa aatctacact tctttcctta cttgctggac ttgataaacc
      721 tcaaacagga aaaatattgt ttaagaatga agat
//
LOCUS       DAYYME010000311          753 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000311 DAYYME010000000
VERSION     DAYYME010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.318"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..271
                     /locus_tag="ACHBFW_002996"
     CDS             <1..271
                     /locus_tag="ACHBFW_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9319212.1"
                     /translation="EQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQKEMAEYLNVS
                     ENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            536..>753
                     /locus_tag="ACHBFW_002997"
     CDS             536..>753
                     /locus_tag="ACHBFW_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319213.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRV"
BASE COUNT          316 a           79 c          157 g          201 t
ORIGIN      
        1 agaacaggag gaaatgtttc taaagcagtt ttatcaaatt gctacaaaag ctattgaaaa
       61 ggctcaagaa aatgcgggga tgacaaggga atatctcaat caaaaagaaa tggcggaata
      121 cttaaatgtg agtgaaaact ttttaaaaaa tgctgtgaga aatgagggat tgcccgtagt
      181 tgaattaggg gcaagaaaat tttattcgaa aaaggcagtt aatgagttta tgtttgctcg
      241 aatgaaaaga ggggaaaata ttggaagatg atgaagtaga aattataaaa aaatgcttta
      301 aaaaaagaaa aaaagtgttc ccaaaagttt gcagactggg ggaacactca ataataaagt
      361 gctgagaaaa ctttatttat agctattata tcaaaaaaga aggtgaaaag gtgttttctt
      421 ctggaaatgt tgtagttgac aaacttagag aaataaattt tgacggaaat gtaatccaca
      481 cacatggtac gatcaattaa gaaaaaaaac gaaaaaaggt gttgagaagc cctatttgaa
      541 tgcaatagtt atcttagcag aaattaccta ttggtataga cctaaaaaaa tttttaacga
      601 tgatggtcag ctacttggtt atcaaaaaaa gtttcttgaa gatattttgc aaaaatcata
      661 taagcaatta agcaaaaaaa caggattatc agaacgagct ataaaagatg caattgtgta
      721 cttagagaaa aaggggatta tcaaaagggt ttt
//
LOCUS       DAYYME010000312          741 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000312 DAYYME010000000
VERSION     DAYYME010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.319"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..680)
                     /locus_tag="ACHBFW_002998"
     CDS             complement(<1..680)
                     /locus_tag="ACHBFW_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9319214.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVS"
BASE COUNT          259 a          149 c           95 g          238 t
ORIGIN      
        1 aatgaaactt tgtattgaga ttggtcaatt cttactcgtt cagtagttag tttataacat
       61 attaaataac aaataagtgc acatatcgaa aatattcccg caacaattgt aaaagttgat
      121 ccaccacgaa ctatttgagc gcctgaatca tctcttgtat aaataaataa tggtgttaat
      181 acaccaatga ccaaacttgc taaagtagct ccaacatttc ggaatgtcga tagcattgtt
      241 ctttcttctg taatagaagt aatagctgat gccattgatc cataaggaat atttatagat
      301 gtgtaaaaaa tactccccca taatatatac gtaacataca tgtatactac cttaacgctc
      361 atagaagctg aaatcataga agtttgatac attaaaaaac ttgctaaagc gactggacca
      421 ctcattctta aaatccacgg tttaaactta ccctgcttag ttgctttcga tctatcaaca
      481 atacgcccca tagttacatc agtaactgca tcgataaatc ttgctactaa aaataatgtt
      541 ccaacggcag ctccgcttat ccccaatact tttgtataaa agatcattaa aaaagaacta
      601 gcaaaaatga aagtaaaatc gtttccaaaa tctccaaaca tatatcctat tttgtctctt
      661 ataccaaatt ttctttccat acaattctct cccttctgtt gttaagcgtt ttcattctga
      721 tgaaagtacg tactatactc t
//
LOCUS       DAYYME010000313          712 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000313 DAYYME010000000
VERSION     DAYYME010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.320"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(245..358)
                     /locus_tag="ACHBFW_002999"
                     /pseudo
     CDS             complement(245..358)
                     /locus_tag="ACHBFW_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          261 a          106 c          121 g          224 t
ORIGIN      
        1 aaagagtata cgaacaaacg ttctttttgt aaaggtggaa tttattatac taaataaata
       61 ggtgaacaag tacttgtgcc aaattatgtg ccaaaaaaaa tcgaatttaa taaaacacaa
      121 acaaaaagaa atcctattat gataacattt cttataataa caaacacaac agaagacgtg
      181 taatagttag tcaggaacgt acaaataacc cctgtatcct ttgcggtaca gggggttatt
      241 ttgtataagt agacattgta gtttctaaac tgctatgtcc taaacgtttc gatacgctaa
      301 gtatatttac tccttgataa agtaaaatag atgcatgcgt atgtcttagt ccatcaataa
      361 acagtcgaag agaaaatagg gaaaaattga ttactaaaga aaaacttact aggtcgacgg
      421 tgtagttgat tttcatactt caatagattc gaattaaacg atttcgattg gcttatacca
      481 gtcctatcca acgaaagata tatcgttctt aactctagcg gtataacttg aagctttacg
      541 taaagccaat gggaatgaac tggagatgac tttttattat ttttagtaat agctgatggg
      601 ttaatactta caactttcct tttcaaagta ccatataaaa aaacggtggg gttgtttagc
      661 gaaataaagg atctaagcca atagcttaga tcctttattc tatgctttat at
//
LOCUS       DAYYME010000314          706 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000314 DAYYME010000000
VERSION     DAYYME010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.321"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..639)
                     /locus_tag="ACHBFW_003000"
     CDS             complement(64..639)
                     /locus_tag="ACHBFW_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFM9319215.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          237 a          152 c          124 g          193 t
ORIGIN      
        1 caaataataa aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt
       61 tattcagcct atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa
      121 tgaaacaacg acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat
      181 cacacttgtt gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc
      241 tgaggaggtg atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg
      301 taccgacaaa atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata
      361 tcttggcaaa atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag
      421 agaaaaaata tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc
      481 aggtacaggc ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg
      541 caaacgaagc cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat
      601 cactgtaagt acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc
      661 tttccaaatc tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DAYYME010000315          704 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000315 DAYYME010000000
VERSION     DAYYME010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.322"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(356..580)
                     /locus_tag="ACHBFW_003001"
     CDS             complement(356..580)
                     /locus_tag="ACHBFW_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319216.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT          260 a          104 c          127 g          213 t
ORIGIN      
        1 ttagaggaat aaataatagt ggagaaaact atggtttggc gcagattcta tcaggtttga
       61 tggaacgaag acaacctccc gacatcaagt caaaagccac ttgagatttt gatctgaaaa
      121 taaaaaaaga aaacaaggta ggaacatcaa tgtttctacc ttgttttcgt aatcaatggg
      181 aaaaaactaa gagtggtgta cgtaatcaat aaaaaggatg gtgacaacag tacctcttta
      241 ctctctgaaa gcatcataac tcgtttttcc tcattatgcc aataaattgt tgttatacca
      301 catataagga tttgtaatag ctaaaaacag acaggaaagt tgaaatctgt gaatcttatt
      361 ttgtatggaa ggatttatag aacgcaaaaa tcaagataaa gatgaataaa aatcctgttt
      421 cggctatata ttgcgtatct acgccaataa aacgagtttg ttctgtcata gccatataaa
      481 gcaaaggcaa aaatacaact acagcgagat aagatccgat gatttctcga aaagttttcc
      541 aggaagtatg tttcctgttt ttatatttat ccgatttcat tgaatagtca tagcctcctt
      601 attctttcat atgttgaaat tccagtataa aaactggaag gaactgtacg ttttttagaa
      661 taaaaattat aagggataag taggctgctg tctataataa gata
//
LOCUS       DAYYME010000316          702 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000316 DAYYME010000000
VERSION     DAYYME010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.323"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>702
                     /locus_tag="ACHBFW_003002"
     CDS             <1..>702
                     /locus_tag="ACHBFW_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM9319217.1"
                     /translation="YLILSFISTFLNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIG
                     LVIYLRIMIKGAHFFDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGG
                     IYSYISNIGIILSVIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVM
                     VVSIDLAKFMAPKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVAS
                     AIVNILLNMIFIPRLG"
BASE COUNT          217 a           87 c          130 g          268 t
ORIGIN      
        1 tatttgattt tatcttttat atctactttt ttgaatattg caatctctgt agctttgatg
       61 cagacattat attttgatga taaatatgtt ggcagagtag tcggcggaac actgtcattt
      121 gtgttgatag gtttagtaat ttatttaaga ataatgataa agggtgctca tttttttgat
      181 aaaaaacagt ggagatacgc tttaaaaatc tctttgccgc taatcccaca ttccttagca
      241 aatatagttt tagcacaatt cgatagaatt atgattaatt cttatttagg tcctgctgcg
      301 gggggaatat attcctacat tagtaacata ggtattattc taagcgtaat atggatgtct
      361 actaataacg cctgggttcc atggttctat ggtgagatga gtaaaaataa tgaaaaaaaa
      421 gtgaagaaag ttagtaacta ttatttagta gtctttactt tgattacgat gattgtaatg
      481 gtagtttcga ttgatttagc aaaatttatg gctccgaaag actattattc tgggttatct
      541 ttagtggttc cgataacctt aggctatttc tttcagtttt tatatagttt accagttaat
      601 gctgaatttt atgagaaaaa aacatcgttt attgcgattg gtacagtagc aagtgctata
      661 gttaatatac tattaaacat gattttcata cctaggttag ga
//
LOCUS       DAYYME010000317          696 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000317 DAYYME010000000
VERSION     DAYYME010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.324"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACHBFW_003003"
     CDS             33..656
                     /locus_tag="ACHBFW_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319218.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
BASE COUNT          244 a          108 c          151 g          193 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctat
//
LOCUS       DAYYME010000318          678 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000318 DAYYME010000000
VERSION     DAYYME010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.325"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..490
                     /locus_tag="ACHBFW_003004"
     CDS             <1..490
                     /locus_tag="ACHBFW_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319219.1"
                     /translation="VSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLL
                     INHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITN
                     RNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDR
                     IE"
     gene            complement(544..>678)
                     /locus_tag="ACHBFW_003005"
     CDS             complement(544..>678)
                     /locus_tag="ACHBFW_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9319220.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          229 a          103 c          123 g          223 t
ORIGIN      
        1 tgtttctttt tttagagaaa actatccagc attttattgc tatttgatag agtatataaa
       61 tgatctaacg tcaaaacgca aagagttgct tcaagataaa ttttttcttt ttttcagtta
      121 tttattatta ttgatcaatc acatacccat tcaactgatt tctgaaccag taaagatcat
      181 catcgatttt tcttatggtg ctgcctataa tcaatttatc aagaaaaatt taagcttgta
      241 cattaatctg aacacggaag tgatagaggc aggaaagcct gagttcccgg atgttataat
      301 cacgaatagg aataatctat atgaagaaag tgcatctcaa gtagtggttt ggctcgatcc
      361 gccaagagca gtagactggg ggaatcttac aaagattttg ttgactatcc aagaagagaa
      421 atacaaagac agcaaaattg acaagcaaat agacgatttt cctgaagaaa tcatcgacag
      481 gatcgaatag tgaacttctt ttagaaagtc cgactgcttt ttctaaaaat attatttgtc
      541 aaattaagcg agacaaaact ttccatttac gtaactcaaa aaaacttcta atggtgtttg
      601 gtaatttagt gattttctag ggatatggtt tcttttggac gcaacggatg agatgaatcc
      661 ttgattgact tggttgaa
//
LOCUS       DAYYME010000319          629 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000319 DAYYME010000000
VERSION     DAYYME010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.326"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..446)
                     /locus_tag="ACHBFW_003006"
     CDS             complement(<1..446)
                     /locus_tag="ACHBFW_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9319221.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          185 a          115 c           86 g          243 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacct
//
LOCUS       DAYYME010000320          623 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000320 DAYYME010000000
VERSION     DAYYME010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.327"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..623)
                     /locus_tag="ACHBFW_003007"
                     /pseudo
     CDS             complement(1..623)
                     /locus_tag="ACHBFW_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          220 a           97 c           95 g          211 t
ORIGIN      
        1 tcacagaagt tgcaggaaaa ttctctttac atatttttaa tagaaaatat aatttatcta
       61 tcataccgct atttagtcgg tcaagtactt cttttttaaa aaaagtattc atcataaaag
      121 ccactacttg cggatcttca tcataaaatt ctgtagcagt acttgaaaag atgttccaat
      181 aaatatctaa tgctgcatat tgaatttgta cctcttttcc agaaaaagac tttctgccat
      241 tattagaaaa ggagatagac aaaccgtact atgctaaaaa tgcattaatc tttttcattt
      301 tcgtatacaa ataagaggca ctatagtttg tttgctgact gaaactgata atcgaatttg
      361 tctgacagct gaacaaagaa gtcaagatct gataactgac tgattcgttg cagtatcttc
      421 caaatagacg ataatagcag taagccggat caattccttc aaaattcaac tttaaaaaag
      481 gtttttgctt atcaatatgc agttctgtta gattgagacg ctcaaagtct tgttctaatt
      541 ctttgatgta tagtaaaaaa tttgattcag acatatggaa atgatctgct aaatattgtg
      601 tagtaataaa gaaacgatgt tca
//
LOCUS       DAYYME010000321          615 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000321 DAYYME010000000
VERSION     DAYYME010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.329"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..426)
                     /locus_tag="ACHBFW_003008"
     CDS             complement(<1..426)
                     /locus_tag="ACHBFW_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319222.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSE"
     gene            complement(443..>615)
                     /locus_tag="ACHBFW_003009"
     CDS             complement(443..>615)
                     /locus_tag="ACHBFW_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9319223.1"
                     /translation="ISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQY
                     QLGLVDNFNNIS"
BASE COUNT          218 a          112 c           61 g          224 t
ORIGIN      
        1 ttcactcaaa tacttataat ctattaactt gtagttgttt aacttttctg aaaaagttag
       61 acccacgtaa taatcttccc agctttctct ttcaattaac tgatccaatt tttgataatc
      121 cataaattca tcatgtatca aaaagaataa ataacttgat attttagcat ctagcttaat
      181 cagattaaat aatgtttgtt taaaattgct accattaata tgactgatag attctctagc
      241 ttcttcacat aaacttttta ctttattaat tactactttg tcatattcat ttaacacact
      301 attattacca ttacttagac gctcagattc tacgaaacaa ctcaaatcag aaatttccca
      361 aaaaaatttt ttgttaaaat ccaatttcct caactcctcc aaactacgta aatgatatag
      421 tctcataata aaataaaacc tctcagctaa tgttgttgaa attgtccact aaacctaatt
      481 gatattgctt aggagataca cctgcgtact ttttgaatac tctcgaaaag tgtgaagaat
      541 ctgaaaatcc taaacctaaa gaaacttcac tgatagtatg gtttgatttc aacattattt
      601 tagccgtaga aattt
//
LOCUS       DAYYME010000322          610 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000322 DAYYME010000000
VERSION     DAYYME010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.330"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..411
                     /locus_tag="ACHBFW_003010"
     CDS             <1..411
                     /locus_tag="ACHBFW_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319224.1"
                     /translation="ETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFE
                     VLLLENDFSSTLLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTN
                     IVLFCEHYNRLSEEKILLPENWAELRKLVEYGII"
     gene            398..>610
                     /locus_tag="ACHBFW_003011"
     CDS             398..>610
                     /locus_tag="ACHBFW_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM9319225.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAI"
BASE COUNT          213 a           88 c           88 g          221 t
ORIGIN      
        1 gaaacagata ttttcgctaa agtaaatagt atattttctg gaaaaaaaga aactttaatt
       61 aagcttcatc ctagagcaag gacaaatcgt tatggtgaaa aaaacatatt gaattccagt
      121 ttaccgtttg aagtcttgct acttgaaaat gacttctctt caacactttt gattagtcct
      181 atatctacaa tttctttttc accatctatg atgtttgaaa agacgatcaa ctctattatg
      241 ttggctaaat taattttaaa tgaatataca gcattatctg aagataaaaa agaaacattg
      301 acaaatattg ttcttttctg tgaacattat aaccgtctat cagaagaaaa aattttactc
      361 ccagaaaact gggcagaatt aagaaagttg gtagagtatg ggattattta acacactttc
      421 caagtcaaaa ttagttaagt ccagtttctg gtatactata gggaattttt ttattaaggg
      481 aatttcattc ataactattc ctatttttgt aaatattact actcaagcag aatatggttt
      541 gattaataat ttcacttcat ttgtatcaat tttttcgatt cttgttgggc taagcctaaa
      601 ttcggcaatt
//
LOCUS       DAYYME010000323          605 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000323 DAYYME010000000
VERSION     DAYYME010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.331"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(122..499)
                     /locus_tag="ACHBFW_003012"
     CDS             complement(122..499)
                     /locus_tag="ACHBFW_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319226.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
BASE COUNT          177 a          106 c          108 g          214 t
ORIGIN      
        1 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
       61 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
      121 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
      181 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
      241 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
      301 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
      361 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
      421 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
      481 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt
      541 aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc
      601 gtttc
//
LOCUS       DAYYME010000324          604 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000324 DAYYME010000000
VERSION     DAYYME010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.332"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..569)
                     /locus_tag="ACHBFW_003013"
     CDS             complement(204..569)
                     /locus_tag="ACHBFW_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319227.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          235 a           83 c          112 g          174 t
ORIGIN      
        1 atattataga tgtttccacc tcaaattcaa tatctattat tggaatcatt attgctgcaa
       61 ttattacaca actgttttta gtgtttggag agaagaaaag aagtaaaaaa tagaaaaact
      121 agtgatagtt ttggtgatat aagaaaaaca tatatgtata aaaagaagaa agtagcttct
      181 ctttgtacat atatggtttg tttttatgga tgccgagctt ttttataaga taaggctacc
      241 gcccataacg acccaaggat ttgtggaact aagtaaatac ctgcaagaag caagatcagc
      301 tgggtcggtt ttaaaggatg atagaagtaa cgccattcag taaaattggc gcaaaataaa
      361 taggaagtga cagtagatat aatattggca atgaataaag atataatacg cctgttcctt
      421 tgaaaataat atccagcaaa aagagataaa aatatcaatg tgatgaaccc gcttacggaa
      481 tgataacgaa aatcaagcag taaggcgaaa tgcacataag gaacaaaaga taaaagcaac
      541 ggaaggtttt tccagcaagt ttttatcatc tttcatcacc acctaacaag tgttattatt
      601 ttgt
//
LOCUS       DAYYME010000325          595 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000325 DAYYME010000000
VERSION     DAYYME010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.333"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACHBFW_003014"
     CDS             <1..435
                     /locus_tag="ACHBFW_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM9319228.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT          193 a          133 c          112 g          157 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaa
//
LOCUS       DAYYME010000326          586 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000326 DAYYME010000000
VERSION     DAYYME010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.334"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>586
                     /locus_tag="ACHBFW_003015"
     CDS             <1..>586
                     /locus_tag="ACHBFW_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9319229.1"
                     /translation="NNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINF
                     DVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFF
                     TESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKT
                     NLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVI"
BASE COUNT          231 a           65 c          115 g          175 t
ORIGIN      
        1 aacaataacg cattaaggaa atttgacatt tttaatagtt cgattgaagt tccctatatc
       61 gaaaaggtaa gtgcagatgt attaaaagtt gaagaaatta cttatgaaaa tattctaaaa
      121 gacataaatt ttgatgtcaa agttggagat gttattggtt tggtagggaa aaacggtgtg
      181 gggaaaacaa ccttactaag aatactcatg aagattattc gccctaccaa aggaaagata
      241 atcgaaaata aaaaaaattt gtcatcgccg tttttagtaa tgcaagaaat ggattaccaa
      301 tttttcacag aatcagtgag gagcgagtta gccctgggga atgaaatagt tagtaaagat
      361 cagcaagaaa aaattttaaa aaaaatggaa ctttataaaa tgaaagatca attaccgttt
      421 gatttatccg gtggagaaaa acaaagatta ttggtttcta tagcttcatt gtcaaaaact
      481 aatttatttc tatttgatga acctacaagt gggttggatt atattaatat ggaaaggatt
      541 gctgacctta ttaaagactt aaaagaaaag ggtgcagtag ttatag
//
LOCUS       DAYYME010000327          564 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000327 DAYYME010000000
VERSION     DAYYME010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.336"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..292
                     /locus_tag="ACHBFW_003016"
     CDS             <1..292
                     /locus_tag="ACHBFW_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM9319230.1"
                     /translation="NETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSN
                     KNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(328..>564)
                     /locus_tag="ACHBFW_003017"
     CDS             complement(328..>564)
                     /locus_tag="ACHBFW_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319231.1"
                     /translation="LKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRML
                     EKRKCADYIPNETITKTDMALMNREAEKIINKLT"
BASE COUNT          180 a           95 c           82 g          207 t
ORIGIN      
        1 taatgagaca caattatggt ttaaatttga cccttcgaat agatttatca aagattttta
       61 taaagtatgg aattcagaag ttttcttttt agcaatcgaa gatagcttat taatcaatct
      121 ctactattct aataagaact atttcaaaat tcctgctgcg aaaactagaa tgaagaaggg
      181 tgtatacttt ttgtttgata tcgtgactga cgtgccggac gcacgtagcg atcatcggcg
      241 ttatgactat ataaagtata ctttcgttga tccagaaaga tacaaagatt aaataaaaaa
      301 ataacaaaca aactcgcttg aaattcatta ggtgagtttg tttattattt tttctgcttc
      361 tcgattcatc agcgccatat ctgttttcgt aatggtttca ttaggaatat agtcagcaca
      421 ttttcttttc tctaacattc tatacccact attatgtatt cctaaggtat tcaattcctt
      481 aactgttgaa aacaaattcc aaacggtttg gtggcttcct tttccgctac ttccattata
      541 aataatatca taattttctt tcaa
//
LOCUS       DAYYME010000328          555 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000328 DAYYME010000000
VERSION     DAYYME010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.337"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..443)
                     /gene="dfr"
                     /locus_tag="ACHBFW_003018"
     CDS             complement(<1..443)
                     /gene="dfr"
                     /locus_tag="ACHBFW_003018"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DfrD/DfrG/DfrK family trimethoprim-resistant
                     dihydrofolate reductase"
                     /protein_id="HFM9319232.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKND"
BASE COUNT          166 a          121 c           56 g          212 t
ORIGIN      
        1 ttatcattct ttatcccttt ttgggcaaat acctcattcc attcctcata attcacttct
       61 ggaaaaaaag tatctccttc gaattcatga tgtatttttg tgatgtacat tttctcaaca
      121 taagggaaaa acaaattata aatctgttct cctccgaaaa taaaaatttc ttcttcgttt
      181 ttacataact caaaaacatc ttctattgaa tgaacaattt cacaaccatt aaaggtaaac
      241 cccttatctc tcgtcagaat aatatttctt ctgtcaggta aggctcttcc gattgattca
      301 aggttcttcc tacctaatat tatcggatgt ccctttgtag tatttttaac atattcccag
      361 tccttgggaa tcctccaagg aatgtcattc tctttgccaa tcactctatt cttatccatc
      421 gcagcaatca aagaaacttt catttataac acagcctttt taactaattt accatctaat
      481 ttcttatttc tatcaaaaaa ccttttctca ttatcctgcc cgttagttga ataagcaatt
      541 ttttctataa gttat
//
LOCUS       DAYYME010000329          536 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000329 DAYYME010000000
VERSION     DAYYME010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.338"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..>536)
                     /locus_tag="ACHBFW_003019"
     CDS             complement(60..>536)
                     /locus_tag="ACHBFW_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9319233.1"
                     /translation="HEVDAKGIIRQKKLYLDPFMDMFNSEILSYRISEKPNALAVMEG
                     LEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLKEYKIFQSMSRKGNCLDNSLMENF
                     FGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNERIKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          153 a          102 c           90 g          191 t
ORIGIN      
        1 gttagacttt tttggagtgg agaaatccac tccttttttc atgctctaaa atgatatttt
       61 tacacaactg ttacggttgt tttacgaaat tggacgggac tttgccagtt taatttctgt
      121 tttatccgtt cattattgta gtaatagata tagctatcga tggctgattt taattcctcg
      181 aaacagttat aaactttccc gtgaaaaatc tcttgtttta ataagccaaa aaaattctcc
      241 ataagtgaat tatctaagca gttccctttc cgagacatgc tttgaaagat tttgtactcc
      301 ttcaatttgt ttctataagc tttcatttga tacgcccaac cttgatctga gtgaaacgta
      361 cgacgatagg gacaatcgtt agtggtctgg atagcttcct ccaaaccttc cattaccgct
      421 aatgcattgg gcttttcaga gatacgatag gataaaatct cactattgaa catatccata
      481 aaaggatcta gatacaattt cttttgacgg ataatgcctt tggcatccac ttcatg
//
LOCUS       DAYYME010000330          534 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000330 DAYYME010000000
VERSION     DAYYME010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.339"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>534
                     /locus_tag="ACHBFW_003020"
     CDS             <1..>534
                     /locus_tag="ACHBFW_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM9319234.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DAYYME010000331          514 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000331 DAYYME010000000
VERSION     DAYYME010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.340"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            150..383
                     /locus_tag="ACHBFW_003021"
                     /pseudo
     CDS             150..383
                     /locus_tag="ACHBFW_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(431..514)
                     /locus_tag="ACHBFW_003022"
                     /pseudo
     CDS             complement(431..514)
                     /locus_tag="ACHBFW_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370675.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
BASE COUNT          198 a           98 c           82 g          136 t
ORIGIN      
        1 aaagaaaaat acaaaggaca aatttgcttg atttacaaag ggactcttca gtacacacca
       61 gaagaatatc cacatcttcc aatatattaa aagaaccttc ttgaatcatt gagtgaaaga
      121 aaagagattc gtcactattt gaggactgtt taactagaga cattagcgat ctgcctgaaa
      181 cctatacaac cactttacga acgtttaaaa attttctgcc acagattcaa aatgcgctta
      241 actgcagcta ttcaaacaga ccattggaat gtttaaacaa ccacatcaaa gtattaaaac
      301 gcaatacgta tggctttcgc agcttttaca atttcaaact acggatcatg attcgtcatg
      361 gcaaaacatt tttaaccaaa taaacagaag aatgtgggat aaatctaaaa atcagttttg
      421 ctacagactc tcgagtaaac ggcgggagca gaagcaactc cttcggaaat aagccgaaat
      481 tcacaaaaat ttgaaaagca attttcgtga attt
//
LOCUS       DAYYME010000332          511 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000332 DAYYME010000000
VERSION     DAYYME010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.341"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..427
                     /locus_tag="ACHBFW_003023"
     CDS             86..427
                     /locus_tag="ACHBFW_003023"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319235.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
BASE COUNT          177 a           55 c           90 g          189 t
ORIGIN      
        1 aagatgtaga gttatttgca ctgttgtgtt ggtatatctc ttaatgctgt gtctttttgg
       61 ggagattaat tagtggagtg attatatgag tatagatgat tgtttagaag aacaaataag
      121 attattttta aagtttttaa aagttaaagt tcaaaataac tacttcacat gttatagtta
      181 tgcacaccct gttttaaata gtcttccttt taattcttta gtgcaaggat tgattacaaa
      241 tattgtttta ttagtctgta caccaaatga aatatttgta aaaaagatag gcagtggtat
      301 ttcttcagta agattaaaag agaaagaata tactcaaaag attattcata ttcctaagcc
      361 tgaaattaag cgttttgaga taaaagacta ttctgcttta gggctgttct caattggtta
      421 ctggtgataa aaaataagaa tactaaaact atacgtagaa tataagtaat cggcatcaat
      481 cctaattatg tgattgaatg ccgatttttg t
//
LOCUS       DAYYME010000333          505 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000333 DAYYME010000000
VERSION     DAYYME010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.342"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(106..405)
                     /locus_tag="ACHBFW_003024"
     CDS             complement(106..405)
                     /locus_tag="ACHBFW_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM9319236.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          157 a           98 c           60 g          190 t
ORIGIN      
        1 ctttatttag aataaaaaaa tacagactct gttcaaataa atgaacctag cctgcattat
       61 gagattgact atacttttta attcattgag atgaataact cgtttttata aatgcccaaa
      121 agtaatcggt gccgaagcca ttcctcgacc taattctttt tcaaattttc ctattgttga
      181 tatctccata taaaacggtg atactggttt agacaattct cccctgattg ctaacggacg
      241 taaactacgt tccaaattat ttatgacttt aggataatac tgaccttttt ctaagcctgt
      301 ctttgcgtct atcaaaagta tacgttcctt ttctttgatc tctggattta aaacgagatc
      361 atataatttt agtaccatgt tttctatttc ttcactttta gacatcacga tttcctccca
      421 aaaatatatt tcttcccact acattctata aacaaagtat acgctacatc tagatttttt
      481 taaatcatta ctggattgtt aaaac
//
LOCUS       DAYYME010000334          498 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000334 DAYYME010000000
VERSION     DAYYME010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.343"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          181 a           81 c           81 g          155 t
ORIGIN      
        1 aaatagaaag acatttgggt taaattgtga gataataata aagaagagtt taaagcgcac
       61 cccaaaccac ttccccataa gtgtgttacg ctttaaactc ttttatattt gaagccatta
      121 aaaagcatac catatttttg aaaaaaagtg agaaaaaagg cttacaattg gagtggtagt
      181 taattagtga cttatttttg attttatagc actgatacta taaaatatag atatcatcat
      241 attacacaat cttaatacta acttaaaaaa tatctccttt cacaagtatg gtgataaaat
      301 tcgttccggg ctaccttttt aggtagccta ctttaatctt tatacctttc tggatcaacg
      361 aaagtatact ttatatagtc ataacgccga tgatcgctac gtgcgtccgg cacgtcagtc
      421 atgaatcggc tacactagac tagacagaaa aaataaggtg tgtagaataa taaaaaacac
      481 actggaggat ttttcatg
//
LOCUS       DAYYME010000335          488 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000335 DAYYME010000000
VERSION     DAYYME010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.344"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>488
                     /locus_tag="ACHBFW_003025"
     CDS             <1..>488
                     /locus_tag="ACHBFW_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFM9319237.1"
                     /translation="KNNLKKKMLIPFDSEVKGLLNKLTISNIDSTREKIIKIFDKINN
                     LFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKEQMIEEEKVRREIDRE
                     KKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEEKLKELEVIRENVLQR
                     EL"
BASE COUNT          234 a           51 c           76 g          127 t
ORIGIN      
        1 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
       61 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      121 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      181 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      241 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      301 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      361 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      421 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      481 gagaatta
//
LOCUS       DAYYME010000336          487 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000336 DAYYME010000000
VERSION     DAYYME010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.345"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..>487
                     /locus_tag="ACHBFW_003026"
     CDS             6..>487
                     /locus_tag="ACHBFW_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFM9319238.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     "
BASE COUNT          176 a           62 c           86 g          163 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctattactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctggactaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatata
//
LOCUS       DAYYME010000337          480 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000337 DAYYME010000000
VERSION     DAYYME010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.346"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>480
                     /locus_tag="ACHBFW_003027"
     CDS             <1..>480
                     /locus_tag="ACHBFW_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFM9319239.1"
                     /translation="VIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLS
                     SNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTK
                     KSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          158 a           87 c           86 g          149 t
ORIGIN      
        1 cagtgattat tgcttgtgtt atatggggca taattaatgg ctatactagc caaagagcca
       61 cgtatagagc ggtttgttcc gtcttatttc ctaatggtga ctttcctagt aggagtcgat
      121 tcactcgctt aagttcaaac ttagcttaca ctatcaagat tattcgatac tttttcatca
      181 agaagctcac caaaggtgaa ctagtcggaa ttatagatag ttttcctagt ccattatgta
      241 aaccagttag aaatagacaa gcaaaactat tgaatcaaat agccaaagtt ggctataact
      301 caacaaaaaa atcttatttt tatggtctta aaattcatat gatcgttact aagactggct
      361 ttccgattac ttactcaatc acgaatccag gtgtccatga cgtgaaagtg ttagagactt
      421 tatcggaaga agcgaatctt cccaatatcc taggggacaa aggatatatt agccataaaa
//
LOCUS       DAYYME010000338          462 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000338 DAYYME010000000
VERSION     DAYYME010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.348"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..430)
                     /locus_tag="ACHBFW_003028"
     CDS             complement(<1..430)
                     /locus_tag="ACHBFW_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9319240.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DAYYME010000339          459 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000339 DAYYME010000000
VERSION     DAYYME010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.349"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..450)
                     /locus_tag="ACHBFW_003029"
     CDS             complement(<1..450)
                     /locus_tag="ACHBFW_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9319241.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
BASE COUNT          134 a           89 c           58 g          178 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctc
//
LOCUS       DAYYME010000340          455 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000340 DAYYME010000000
VERSION     DAYYME010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.350"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..416
                     /locus_tag="ACHBFW_003030"
     CDS             117..416
                     /locus_tag="ACHBFW_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM9319242.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          167 a           61 c           85 g          142 t
ORIGIN      
        1 gaaaataaac aacctgtaac tgcaaaaaaa gaaacagtat tctctgaagg caaaataaaa
       61 actcgatata tattagaggt agagactaag tttaaaaata gatcggagta atggttatgc
      121 tttttaatga gacgcaatta tggtttaaat ttgatccttc gaatagattt gtcaaagatt
      181 tttataaggt gtgggattca gaagttttct ttttagcaat cgaagatagc ttattaatta
      241 atctctacta ttctaataag aactacttta aaatccctgc tgcgaaaact agaatgaaga
      301 aagacgtata ttttttgttt gatatcgtga ctgatgtgct agacgctcga agcgatcatc
      361 ggcgttatga ctatataaag tatactttcg ttgatccaga aagatacaaa gattaaagta
      421 ggctacctaa aaaggtagcc cggaacggat tttat
//
LOCUS       DAYYME010000341          454 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000341 DAYYME010000000
VERSION     DAYYME010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.351"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          147 a           89 c           51 g          167 t
ORIGIN      
        1 cacaaaaaat aaaaaaatta ttacgttatt aaaaaaacag atgaaagatc aggttttatt
       61 agatctcatt ctttcatcac ttgaagggat ttaatgaact actttttcct ctttgtcgtt
      121 tttatatatt cgtattcctt caaacagtag gatagcctct ttcaatgctt gatatttgaa
      181 ctgtccgtta cttaaaaatt tagatgtgcc gccctcttcc tagaacatat tcttttttac
      241 tttttcatta attgacagac caatcgaacg aaactgtaaa tttcgatagg ataatgattg
      301 gtcaaactca ttcattttca agacatctcg attcatattc aagataggaa tctttttatc
      361 aacatcactt tattcgtatt ttcctctacc ataaaagctt cacgctccac aaaattgttt
      421 tatacactac tattcagccc attcaacgac caat
//
LOCUS       DAYYME010000342          451 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000342 DAYYME010000000
VERSION     DAYYME010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.352"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>451
                     /locus_tag="ACHBFW_003031"
     CDS             <1..>451
                     /locus_tag="ACHBFW_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9319243.1"
                     /translation="FRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVR
                     TLNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNE
                     LLQDIYSRGVHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRN"
BASE COUNT          147 a           89 c           87 g          128 t
ORIGIN      
        1 tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa atgtatgggc
       61 atcactacac gccccaaacc atgtccaaca tgactaaagt gctgactgaa gaagtaaatg
      121 catttaaagt tagaactcta aatgataagt atgtcgctat tttcatggat gctacttata
      181 ttccattaaa acggcaaacc gtctccaaag aagcaatcta tattgccatt ggtatacgag
      241 aagacggcac taaagaagta ctaagttatg cgattgctcc gactgaatcg acctacgttt
      301 ggaatgagtt gttacaggac atttactcca gaggggttca tgatgtctta ctgtttatta
      361 ccgatggctt aaaaggcatg aaagatacga ttcaccagat ttatcctaaa gctaaatacc
      421 aacattgctg tgttcacgtt tcccgtaata t
//
LOCUS       DAYYME010000343          431 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000343 DAYYME010000000
VERSION     DAYYME010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.353"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..220
                     /locus_tag="ACHBFW_003032"
     CDS             <1..220
                     /locus_tag="ACHBFW_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFM9319244.1"
                     /translation="DYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQAVGSE
                     EEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT          163 a           83 c           71 g          114 t
ORIGIN      
        1 agattacttt actcaggcag acttgatcat tacactttgt ggtgatgcca aagacaagtg
       61 ccctgccgtt ccaaacaatc acgaacacct tcactgggac ctaaaagatc ctgcacaagc
      121 tgtcgggagc gaagaagaaa tattaacgga attcagaaaa gtcagagaag gaatcaaaag
      181 aaaagtttta gacttaaatc aacgtacatc gatcgtataa agaagtattc aaaatgattt
      241 ttcaagcact aagaaacaat gttaaaaaaa tagctttcca tattttacaa aattgtgcct
      301 tattgccgac aaattgtctc ttgaacaccg tttaccagta gtcaagacat cgtaagcaac
      361 tataaaaaat tataagaaac tcaaaataaa aggatgtgcc acaactttgg ccacatcctc
      421 tttttttgta t
//
LOCUS       DAYYME010000344          425 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000344 DAYYME010000000
VERSION     DAYYME010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.354"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          118 a           74 c           80 g          153 t
ORIGIN      
        1 taatagtttt tattcaaaat gtttctagat tataaaattt attgaataac acattcctaa
       61 cggaatttgt ttcgatattg tgtgatttct cagagtacaa tggatgtatg gaaaggatcc
      121 aacgggaatt tgctatttcc tcctgtagcg tttgtctacc ctgtttagtc tgttacccga
      181 accactgtta tcactaccaa ctcattagct gtttcgataa tggactagac tttcaaatag
      241 tagtttggaa ggcagcttct agctctttta tcaccgtatt ctattaggcg aggttgtggc
      301 tatacagtga gtttctggga aatcccacag tatcgaagca ttttccaaaa tttatatcga
      361 aaggaggtcc ttcttccatg aaattatttg tcggtattga cgttagctct gaaaaactag
      421 atgtt
//
LOCUS       DAYYME010000345          414 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000345 DAYYME010000000
VERSION     DAYYME010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.355"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..>414)
                     /locus_tag="ACHBFW_003033"
     CDS             complement(109..>414)
                     /locus_tag="ACHBFW_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9319245.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          116 a           96 c           82 g          120 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggt
//
LOCUS       DAYYME010000346          405 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000346 DAYYME010000000
VERSION     DAYYME010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.356"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..157
                     /locus_tag="ACHBFW_003034"
     CDS             <1..157
                     /locus_tag="ACHBFW_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9319246.1"
                     /translation="PLLKEPEQIFLIEKFIFDKTDKEIGKVLGITRQGATNLKHRLYK
                     KIQKMLV"
     gene            251..>405
                     /locus_tag="ACHBFW_003035"
     CDS             251..>405
                     /locus_tag="ACHBFW_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9319247.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLA"
BASE COUNT          148 a           56 c           72 g          129 t
ORIGIN      
        1 tcctttgttg aaagaacctg aacaaatttt tcttatcgaa aaatttatct ttgataaaac
       61 tgataaagaa attggaaagg ttctggggat tacaagacag ggtgcgacaa atttaaaaca
      121 tcgactttac aaaaaaattc aaaaaatgct ggtttaatat ctgcataata actaattttg
      181 tatgcttctt tgctcgttct cctccttccc aaagaaatag attataagtc catgggaaga
      241 aggtagattt atgaattctt taaaagaaga tttcatttta gcaaaggctg gaaatgaaga
      301 agctgttgag gcaattctca aaagatttag ctctttgatt cataagcaat cctggagaac
      361 tggaaaatat gatcaagatt gttatcaaga atgtatgctt gcaat
//
LOCUS       DAYYME010000347          400 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000347 DAYYME010000000
VERSION     DAYYME010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.357"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>400
                     /locus_tag="ACHBFW_003036"
     CDS             <1..>400
                     /locus_tag="ACHBFW_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319248.1"
                     /translation="LAKWAKSNETYSQVLKRIMSQETSFIYELSKSNLKYTYNEDELE
                     ILDNPENSQWIKELKREDFIETLRILKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQ
                     IEGEDKEKRIDNIINYINSLSEPLKCRGLD"
BASE COUNT          166 a           43 c           74 g          117 t
ORIGIN      
        1 aattagcgaa atgggcaaag tcaaacgaaa cttatagtca agtacttaaa agaattatga
       61 gtcaagaaac ttcatttatc tatgagttaa gcaagtcaaa tttgaaatat acttataatg
      121 aagatgaact tgaaatttta gataatccag aaaattcaca atggattaaa gagttgaaga
      181 gagaagactt tattgagaca ctaagaattc taaaatcgtt atttgatttg aaattagcca
      241 aagctaccat catgggaatt gaaagagatt catattattg ggaggaaatt ggactatatg
      301 ctccccagat tgaaggtgaa gataaagaga aaaggataga caacattatt aactatatta
      361 atagtttaag tgaaccttta aaatgtagag gacttgattt
//
LOCUS       DAYYME010000348          395 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000348 DAYYME010000000
VERSION     DAYYME010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.358"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>395
                     /gene="bsh"
                     /locus_tag="ACHBFW_003037"
     CDS             <1..>395
                     /gene="bsh"
                     /locus_tag="ACHBFW_003037"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bile salt hydrolase"
                     /protein_id="HFM9319249.1"
                     /translation="SFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPG
                     DLSSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYS
                     SCCNVDKGIYYYRTYEDSQITAIDMNKED"
BASE COUNT          132 a           54 c           73 g          136 t
ORIGIN      
        1 cttcatttga ctatcaatta tttaatttaa acaattatcg tgtcttatcg agtgaaactc
       61 ctaaaaataa tttttcaaat caaataagtt tgaatgccta tagccgcggt atgggaggga
      121 taggcttgcc tggagattta tcctcagtat ctcgttttgt taaagcgact tttacgaagc
      181 tgaattctgt atctggagat tcagagtcag aaagtattag tcaatttttc catatcttag
      241 gttcagtaga acaacaaaaa ggtttgtgtg atgttggtga tggaaaatat gaatatacaa
      301 tttattcttc ttgttgcaat gttgacaaag gaatctatta ttatcgaaca tatgaagaca
      361 gtcaaattac tgcaattgat atgaataaag aagac
//
LOCUS       DAYYME010000349          360 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000349 DAYYME010000000
VERSION     DAYYME010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.360"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..327
                     /locus_tag="ACHBFW_003038"
                     /pseudo
     CDS             136..327
                     /locus_tag="ACHBFW_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT          151 a           43 c           57 g          109 t
ORIGIN      
        1 aaacaggata tatagaatca aaatacgaat cttattttga tttttctagt tttttgaaaa
       61 aagcaccctt tgatcgttgt attagtggaa aagagaaaaa ggagcatact tatcatgtca
      121 caaaactaca actaaatatc taaacaacac caatttgatg cctattgaaa aaaagtattg
      181 cgaaatgaag ccaagtctat tagaaaatga aataatagaa taaaagaaaa tgggaaaccg
      241 ctgaatgatg taaatgaagg taagtatcca cgaactaatt ttgatgaaca agatatctat
      301 tttttatttg tattggagat actagtttaa tatcaaaagt tatcagcagt cataattagt
//
LOCUS       DAYYME010000350          357 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000350 DAYYME010000000
VERSION     DAYYME010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.361"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACHBFW_003039"
     CDS             <1..156
                     /locus_tag="ACHBFW_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFM9319250.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..>357
                     /locus_tag="ACHBFW_003040"
     CDS             175..>357
                     /locus_tag="ACHBFW_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9319251.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKD"
BASE COUNT          149 a           31 c           66 g          111 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagat
//
LOCUS       DAYYME010000351          352 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000351 DAYYME010000000
VERSION     DAYYME010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.362"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            16..>352
                     /locus_tag="ACHBFW_003041"
     CDS             16..>352
                     /locus_tag="ACHBFW_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9319252.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFS"
BASE COUNT          140 a           62 c           61 g           89 t
ORIGIN      
        1 aaaaggagaa aaaagatgaa gccaaatcct tcagaagcag gaaaacgaat caaacaatta
       61 cgcctttcat gtggttttac tatggaagaa cttggaagga aaatagataa ttcaccacgt
      121 gcgaccatat ccaactggga acgtgggacc aatttaccaa atccacaaaa gctgaaatta
      181 ttatcaacta tcactaattc aacaattgac tggattaaat ggggaacttt agaagaatat
      241 ataacttcat accttatcga tatagggtat gagctatata ttaaggactt cccagaaata
      301 cctcacaaag tctttaaaga cattcaagaa agatatagtg atacgttttc gc
//
LOCUS       DAYYME010000352          343 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000352 DAYYME010000000
VERSION     DAYYME010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.364"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>343
                     /locus_tag="ACHBFW_003042"
     CDS             <1..>343
                     /locus_tag="ACHBFW_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFM9319253.1"
                     /translation="IPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDAL
                     IQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVK
                     EIANYFSVSETK"
BASE COUNT          123 a           44 c           63 g          113 t
ORIGIN      
        1 attccaagtg ttagtggaga tacgattcgg tctgaaaaaa attattctat cattgttttt
       61 gaaaagttag cacaaacgtc tataacatta ggaatggata taatagaagc gtatcaaagt
      121 agagatgctt tgatacaaga aaacgaatta gctgtatcgt tacctgaggt cttaaaagtg
      181 agggattctg ggattgttta ttatactaag gaaatcggaa aaactaagat agagcatctt
      241 tctcccttaa tttcttctgt cgttcaattc atcggcttaa atatctataa aagaattact
      301 gtaaaggaaa tagctaatta tttttctgta agtgaaacta aat
//
LOCUS       DAYYME010000353          332 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000353 DAYYME010000000
VERSION     DAYYME010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.365"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..104
                     /locus_tag="ACHBFW_003043"
     CDS             <1..104
                     /locus_tag="ACHBFW_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM9319254.1"
                     /translation="EYQEELIRNLSEIGRTAGINGLYRADYGDKSQF"
     gene            208..>332
                     /locus_tag="ACHBFW_003044"
     CDS             208..>332
                     /locus_tag="ACHBFW_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFM9319255.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
BASE COUNT          138 a           30 c           65 g           99 t
ORIGIN      
        1 ctgaatatca agaggaatta ataagaaatt tgagtgaaat tggaagaact gctggaataa
       61 atggtttata tcgagcagat tatggagata aatctcaatt ttaatgatac ttataatatt
      121 aaatatgtct caaaattttt tgattagaaa aataggtata ttgaatcagt gacataaaag
      181 tatttatagt ttgtaggggg aatcgtaatg gaagaaaaaa atagactaaa tgctaaacaa
      241 tgttcagatc aagagcttaa aaaaattaaa ggaggtgctg gaacaaaacc tcaaggaaaa
      301 ccggcatcaa atttagtgga atgtgttttt tc
//
LOCUS       DAYYME010000354          320 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000354 DAYYME010000000
VERSION     DAYYME010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.366"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>320)
                     /locus_tag="ACHBFW_003045"
     rRNA            complement(<1..>320)
                     /locus_tag="ACHBFW_003045"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           74 a           94 c           77 g           75 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct
//
LOCUS       DAYYME010000355          320 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000355 DAYYME010000000
VERSION     DAYYME010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.367"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          115 a           58 c           30 g          117 t
ORIGIN      
        1 tcttttttga atactatatt cgttctgttt ttcatcttca aatacgaact catccgcaga
       61 aatatttgtc acaactccat gaatcttctt ttcattacca cctatatacc acaggattcc
      121 tacacagaaa caacaaaaaa atagcataaa ataggttttc tttattttat atccctcctt
      181 ttagattaaa agctcacaga aaacgctttc taaaaagaca atagaataat cataattttt
      241 cattttgtat tatttataca aataatacgc atgttactga aaaatgatat ggttccctac
      301 tttttgagat aagttaaata
//
LOCUS       DAYYME010000356          317 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000356 DAYYME010000000
VERSION     DAYYME010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.368"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>317
                     /locus_tag="ACHBFW_003046"
     CDS             <1..>317
                     /locus_tag="ACHBFW_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM9319256.1"
                     /translation="EILANNINPLIEEYANQRHSISKELFFKRANSKLQDKIRNGDFK
                     YGEKERMIDSLKQLLDEMDLAYKDISKYSSVEDFFYKITKNQFETEKFISDLSKKYNF
                     PYE"
BASE COUNT          132 a           47 c           45 g           93 t
ORIGIN      
        1 tgagatactt gctaataata tcaatcctct gattgaagag tacgctaacc aaagacactc
       61 tatctctaaa gagctatttt tcaaaagagc caacagtaaa cttcaagaca agataagaaa
      121 tggtgatttc aaatacggtg aaaaagaaag aatgattgat tctttaaaac agctattaga
      181 cgaaatggac ttagcctata aagatatttc aaagtattca tctgtcgagg atttctttta
      241 taaaattact aaaaaccaat ttgaaacaga aaaatttatt tcagatctta gtaaaaaata
      301 caatttccca tatgaga
//
LOCUS       DAYYME010000357          313 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000357 DAYYME010000000
VERSION     DAYYME010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.369"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          102 a           57 c           35 g          119 t
ORIGIN      
        1 tttctcagaa acaaaccact cattggaaaa gacatgaatc aaatcatcac ttacttgtgt
       61 aaaatagcga cgtttaatga caaagatatc ttcattgcta tctagtgtac cttcatttct
      121 ttgtgcttaa attcaaagat aattttctca ataaagattt ttgattccag taagattcct
      181 ttactccttt tacaacatag cgttgtcctt tgttttgaaa catcttcttc ccctctttgc
      241 cattaaaaat agcccaattc tctataaaga agattaagct tcattattat aatataaagt
      301 ttaaatttgg ttt
//
LOCUS       DAYYME010000358          311 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000358 DAYYME010000000
VERSION     DAYYME010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.370"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          125 a           38 c           39 g          109 t
ORIGIN      
        1 ataaaattac tataaaaaag caaaatatta cctcctaatt aaatattaaa taccgaaaaa
       61 attatgttta gataatacca tagttaagtt tttccttgtg atgtttttcg taaaaagata
      121 ttttagcagc attatcgtta cagtgcatag ttaccatcgg cgttatgact atataaagta
      181 tactttcgtt gatccagaaa ggtatataga ttaaaataag ttacctaaaa aggacaaata
      241 caaattttat tatcttattc acagtaagaa gataatttta agattgcgaa atacgatact
      301 atttgtaatc t
//
LOCUS       DAYYME010000359          309 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000359 DAYYME010000000
VERSION     DAYYME010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.371"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..309
                     /locus_tag="ACHBFW_003047"
                     /pseudo
     CDS             1..309
                     /locus_tag="ACHBFW_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DAYYME010000360          307 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000360 DAYYME010000000
VERSION     DAYYME010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.372"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHBFW_003048"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHBFW_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFM9319257.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           96 a           45 c           50 g          116 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttat
//
LOCUS       DAYYME010000361          305 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000361 DAYYME010000000
VERSION     DAYYME010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.373"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>305)
                     /locus_tag="ACHBFW_003049"
     CDS             complement(<1..>305)
                     /locus_tag="ACHBFW_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319258.1"
                     /translation="LKDLKQYYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFF
                     SDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESF"
BASE COUNT          105 a           49 c           42 g          109 t
ORIGIN      
        1 aaaactttca atgaatcgtt cattcaacca ttcgattgtt tcatcttcat tttttctcaa
       61 gtatttttga tccaatttat catgaagata aagccaagag agtgtcttat tgataaaaat
      121 aacggatttt tctcctaata ctccttttaa gtggtaaaat cctatatccg aaaaaaatag
      181 cgactgattg ctagcaatat ctaaagaatc agcttgttca gccaacaggt tgattgctaa
      241 aaagtggaat aatttctttt tttcataagc agacagagga taatactgtt ttaaatcttt
      301 taaca
//
LOCUS       DAYYME010000362          291 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000362 DAYYME010000000
VERSION     DAYYME010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.374"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..>291
                     /locus_tag="ACHBFW_003050"
     CDS             39..>291
                     /locus_tag="ACHBFW_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319259.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESS"
BASE COUNT          134 a           41 c           47 g           69 t
ORIGIN      
        1 caattaatag gaacgtatat tcgaaaggaa tagaaataat gtggttagaa aaaataaatg
       61 acaaaaaatt taagtacacc gaacgttata cggatccctt aactgaaaaa aagagaaaag
      121 tgtccgttac tttatctagc aattcacgcc aagcttggaa tcaagctaac ttacttttga
      181 atgaaaaaat cgcagaaaaa ataaaacgaa atgaagaatt accactaact tttggagaat
      241 taaaaactaa atgggatgaa aaatacaagc caactgtaaa agaaagttcc t
//
LOCUS       DAYYME010000363          290 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000363 DAYYME010000000
VERSION     DAYYME010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.375"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>290)
                     /locus_tag="ACHBFW_003051"
     CDS             complement(<1..>290)
                     /locus_tag="ACHBFW_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319260.1"
                     /translation="QIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIGLD
                     NVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI"
BASE COUNT          121 a           36 c           46 g           87 t
ORIGIN      
        1 gataaaaaaa tagtagatat tataacttaa aacaagattt cccatcattt tataaaataa
       61 aaaagctgga taaagagtaa gccatggata aaaaatattc atcattgttc catgatgatc
      121 gaagttattg aagtttactg gagaactcca tacattatct agtcctatga ttcgagataa
      181 atgaaagtaa gtatcctgat tgacactatg atagattccc ttagacatga atagagggat
      241 gacgtaagca aaacttagaa ataacataaa aatgatatct attatttggt
//
LOCUS       DAYYME010000364          288 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000364 DAYYME010000000
VERSION     DAYYME010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.376"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>288)
                     /locus_tag="ACHBFW_003052"
     CDS             complement(<1..>288)
                     /locus_tag="ACHBFW_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319261.1"
                     /translation="YVFEKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAF
                     SLLTFYPFFQLLRGEEINRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSL"
BASE COUNT          107 a           49 c           36 g           96 t
ORIGIN      
        1 tataaactaa ctatacttgt tagaacaaaa aaacaggcgt tgacttgttc ttgattagtt
       61 aaaaatatca ataaaggaat acttacccaa tatatttttc tatttatttc ttctcctctt
      121 aagagttgaa aaaacggata aaaagttagt aaactaaatg ctactggcca ctgatagttc
      181 aaggtggttg ctctccaacc ggtttccccc attactgtca atggtattaa acagtatata
      241 gagcaaacca ataataaatt tttaacattt attttctcaa aaacatac
//
LOCUS       DAYYME010000365          282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.377, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000365 DAYYME010000000
VERSION     DAYYME010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.377"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>282
                     /locus_tag="ACHBFW_003053"
     CDS             <1..>282
                     /locus_tag="ACHBFW_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFM9319262.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DAYYME010000366          281 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000366 DAYYME010000000
VERSION     DAYYME010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.378"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            130..>281
                     /locus_tag="ACHBFW_003054"
     CDS             130..>281
                     /locus_tag="ACHBFW_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9319263.1"
                     /translation="MEEVCNMSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHE
                     QLDQLPP"
BASE COUNT           96 a           54 c           45 g           86 t
ORIGIN      
        1 aaaaacattt ttgaaaataa acatcagcag tcctatcaat gcatgtgagc ttatagaagt
       61 tattcgcagc gcagcataat tgcattagct tcttttcgtg ttttttgcct cttttccctt
      121 taaatcttaa tggaggaggt gtgcaatatg tcagataacg acgaacgcgt aaatgcccag
      181 ttgattcgat tctttgaaaa ggtcattttg aacaccgcta atacttatta taaaaaacaa
      241 gaagaaattc aaaaacatga acaacttgat caactcccac c
//
LOCUS       DAYYME010000367          273 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000367 DAYYME010000000
VERSION     DAYYME010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.380"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>273
                     /locus_tag="ACHBFW_003055"
     CDS             <1..>273
                     /locus_tag="ACHBFW_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFM9319264.1"
                     /translation="SVDILRPNLPICREQRILVNILARLSITKIESEETSLEQYKLLK
                     KANVPHVLYSREMVESLCTVFDLFIPEKEKILYSLAFARNKLYNGF"
BASE COUNT           89 a           49 c           45 g           90 t
ORIGIN      
        1 tctctgtaga tattttgcga cctaatctgc caatttgtcg cgaacaacgc atcttagtga
       61 atattttagc tcgactttct atcacgaaaa tcgaatcgga agaaacaagc ttggagcaat
      121 acaaactttt aaaaaaagca aacgttcctc atgttcttta ttcaagagaa atggtggagt
      181 ccctttgtac tgtatttgat ttgtttatac ctgaaaaaga aaagattctt tactcattgg
      241 cattcgcacg aaataaactt tataatggat ttc
//
LOCUS       DAYYME010000368          260 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.denovo.382, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000368 DAYYME010000000
VERSION     DAYYME010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.denovo.382"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>260)
                     /locus_tag="ACHBFW_003056"
     CDS             complement(<1..>260)
                     /locus_tag="ACHBFW_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFM9319265.1"
                     /translation="PRINRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHGSQW
                     WALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFF"
BASE COUNT           86 a           37 c           40 g           97 t
ORIGIN      
        1 ttaaaaaaaa gtgtatgaaa aaatttttca tctggggcaa acatacctat tttaaaattc
       61 aggtagtctt gttgattttg ttcatacatt tgtattaatt ctgttaggca ttctttcgac
      121 aaagcccacc attgagaacc atgatacaaa tcaaatttat ggtgtctaat ttttggggga
      181 agtttaatca tcctcagaca acgattaatt atagctctaa aaattttaaa tgcagttgtg
      241 ttattcctat taattctagg
//
LOCUS       DAYYME010000369        15294 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.guided.062, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000369 DAYYME010000000
VERSION     DAYYME010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.guided.062"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..778
                     /locus_tag="ACHBFW_003057"
     CDS             <1..778
                     /locus_tag="ACHBFW_003057"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFM9319266.1"
                     /translation="ADEDAEAVRELPELCEGQTFADVAASVTEHFTTPPKPYTEDTLL
                     SAMERAGAEDMPEDAERKGLGTPATRAAILEKLVQMGFVQRKGKQLIPTKDGINLAVV
                     LPEALTSPQLTAEWENRLTEIAKGQADPDEFMDGIETQARELVQTYSCISEEKQKLFQ
                     AERVPIGTCPRCGEAVYEGKKNYYCGNRACQFVMWKNDRFFEERKKAFTPKIAAALLK
                     TGKVKVKGLYSVKTGKTYDGTVLLADTGGKYVNYRIEHRS"
     gene            927..4847
                     /locus_tag="ACHBFW_003058"
     CDS             927..4847
                     /locus_tag="ACHBFW_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFM9319267.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            4848..5792
                     /locus_tag="ACHBFW_003059"
     CDS             4848..5792
                     /locus_tag="ACHBFW_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319268.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            5796..5999
                     /locus_tag="ACHBFW_003060"
     CDS             5796..5999
                     /locus_tag="ACHBFW_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFM9319269.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(6299..6535)
                     /locus_tag="ACHBFW_003061"
     CDS             complement(6299..6535)
                     /locus_tag="ACHBFW_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9319270.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(6718..7089)
                     /locus_tag="ACHBFW_003062"
     CDS             complement(6718..7089)
                     /locus_tag="ACHBFW_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFM9319271.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(7086..8414)
                     /locus_tag="ACHBFW_003063"
     CDS             complement(7086..8414)
                     /locus_tag="ACHBFW_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM9319272.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(8375..8704)
                     /gene="mobC"
                     /locus_tag="ACHBFW_003064"
     CDS             complement(8375..8704)
                     /gene="mobC"
                     /locus_tag="ACHBFW_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM9319273.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(8962..9332)
                     /locus_tag="ACHBFW_003065"
                     /pseudo
     CDS             complement(8962..9332)
                     /locus_tag="ACHBFW_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            9477..9662
                     /locus_tag="ACHBFW_003066"
     CDS             9477..9662
                     /locus_tag="ACHBFW_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM9319274.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            9820..9987
                     /locus_tag="ACHBFW_003067"
     CDS             9820..9987
                     /locus_tag="ACHBFW_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319275.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            10605..11264
                     /gene="vanR-B"
                     /locus_tag="ACHBFW_003068"
     CDS             10605..11264
                     /gene="vanR-B"
                     /locus_tag="ACHBFW_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM9319276.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            11267..12628
                     /gene="vanS-B"
                     /locus_tag="ACHBFW_003069"
     CDS             11267..12628
                     /gene="vanS-B"
                     /locus_tag="ACHBFW_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM9319277.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(12719..14020)
                     /locus_tag="ACHBFW_003070"
     CDS             complement(12719..14020)
                     /locus_tag="ACHBFW_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9319278.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            14299..15105
                     /locus_tag="ACHBFW_003071"
     CDS             14299..15105
                     /locus_tag="ACHBFW_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFM9319279.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
BASE COUNT         3886 a         3779 c         4038 g         3591 t
ORIGIN      
        1 cgccgacgag gacgccgagg cggtgcggga gctgccggag ctttgcgagg gccagacctt
       61 tgcagacgtg gccgcctctg tcaccgagca tttcaccacg ccgcccaagc cctacaccga
      121 agatacgctt ttgtccgcta tggaacgggc cggggccgag gatatgccgg aggatgccga
      181 gcgcaaggga ttaggcacac cggccacccg cgccgccatt ctggaaaagc tggtgcagat
      241 gggctttgtc cagagaaaag gcaagcagct cattcccacc aaggacggca tcaatctggc
      301 tgtggtgctg ccggaggcgc ttacttctcc ccagcttact gccgaatggg aaaaccggct
      361 gaccgagatc gccaaggggc aggccgatcc tgacgaattt atggacggca tcgagacgca
      421 ggcgcgggag ctggtgcaga cctattcctg tatcagtgag gaaaagcaga aattgtttca
      481 ggccgagcgt gttcctatcg ggacgtgtcc ccgctgtggt gaggctgtct atgagggcaa
      541 gaaaaactac tactgcggaa accgggcctg ccagttcgtc atgtggaaga atgaccgatt
      601 ttttgaggaa cgcaagaaag cgttcacccc gaagatcgcc gctgcgctgc tcaaaaccgg
      661 caaagtcaag gtaaagggcc tctattccgt aaagaccggc aaaacctacg acgggacggt
      721 gcttttggct gataccgggg gcaagtacgt caactaccgc attgaacaca ggagctaaag
      781 gacagtaaca cagcaagcat agggagtgtg gctacactcc cggtaaaatc ccccggccgc
      841 gtccctgccg ggccggggat ttttgcttgt tggggaagtc cccaaccccc tattttttga
      901 aacagaacag agaaaggagt gaccgcgtga acgaagtatt ttctatccaa gtccataacc
      961 ggcagttttt ggaacagggc aaacaaggcg tctggctgga tttgcccacc acaaccgaaa
     1021 cgctgcaagc cgccctgcgg gatgttggca ttactgccga caatccgcag gattttttta
     1081 tcaacgggta ttcctgcccg gaggacagac accttgccat tccctatgac atggtgctgg
     1141 ccgccgacgt ggacgagctg aatttccttg ccgcccggct gggccagctt gacgccgccg
     1201 agatcgcaga gctgaacgcc gccctgcaaa atccccgtgg cggctttgag aatatcggcc
     1261 agatcatcga ctacaccgaa aatgtggatt actatgtgca tcttcccgac gttagggggg
     1321 atggacagtt gggcgactac tacctgaacc gttccggcat ggtggatatg ccggaggaat
     1381 ggaaagccgg gattgatacg gcgcagtttg gacgccacat tgcccagcag gaacaaggcg
     1441 tttttaccca gtacggctat ctggtgagaa gcggcgacga atggcagcgc gtccatgagg
     1501 gccagccggt gccggaggaa taccgggtga tgcactaccc ccagccggaa gctgaccggg
     1561 aggaaccgca ggcccagcag ccggaggcat ccgaaagccc cgacaaagcg ccgcccccgg
     1621 ttatcccgat catgctggac ggcatgaaca gcaaagaacg catgaaagtg attactgacc
     1681 gtctggaaac cggcgtacag gagctttttg aaagtagccg ctatatggct tacttatccg
     1741 ctatgtccaa gatgcacgat tacagtctga acaatacgct gctgatcgcc atgcagggcg
     1801 gttcgattgt ggcgggctat aacaggtggc aggatgaatt tgaccgccat gtgaagcggg
     1861 gcgagaaagg cattaagata ctggccccgg ccccgttcaa ggttaagcag cagatggaga
     1921 aaaccgaccc ggccacccag cagcccgtga tcggcgcgga cggcaagccc gtgaccgaag
     1981 aacaggaagt gacggttccg gcctttaagg tggtgacagt ctttgacatc agccagaccg
     2041 agggcaagga ttttcccatg ccctatatca gtgaacttac cggcgacgtg cagcagtatc
     2101 aggatttctt tgccgcgctg gaacggacat caccctatcc cataagcatt gagccgattt
     2161 cagacggggc caagggccgc tgcttctacg caaaacagcg cattgccatc aacgagggca
     2221 tgagcgaatt gcagacccta aagaccgcca tccatgagat tgcccatgcc aagctccacg
     2281 ccattgaccg ggaggccccc gccgaggaac aggcggcccg gccagaccgc agcacccgcg
     2341 aggtacaggc cgaaagcgtg gcctataccg tctgccagta ttacgggctg gacacgtcgg
     2401 actattcttt tggctatgtg gccgattgga gcagcggcag ggagttatcc gagctgaaag
     2461 cctcccttga aaccatccgg gccacggcca gcgaattgat aaccgagatc gacggccatt
     2521 ttgcccagct tcagcaggag cgcgaggccc ggcaggaaca ggaggccgtc acggttgacg
     2581 ttaccccgga ggcagcggcc ccggcgcagg ccgaggaaga acaggccccg gcccttgatc
     2641 cggcagccga gccgcttgtg actgtgctat ggagcgaaag cccccagcta aaggacggcc
     2701 agcaaatgcc gttgcatgag gccgacgcgc tgtttgcccg tctggatgca gcatatccgg
     2761 cagaggcagg ctatgacaaa accgcgttcc gcattgactg tatctttcag ggcgagccgg
     2821 acagctacga ggggcggcag gattttggag atagggacgg ttccctgatc gaacatatcc
     2881 agagctacca ccaattttac gcgcaggacg aaaactggaa aaagcatgta atcgctacaa
     2941 ccggcataga gggctgggag caggacaagg cccatcggga tatgctgtta ggcgagtttg
     3001 tcccctatat gaagctgcat tgcagccttg cccaaatgga acagacggcg cagggccgct
     3061 tacagtccgg ggaaaccctg acgccggagg aaacggccta tttgggggcg cttctctctt
     3121 atgtgcagga gtgccgcccc atgctcaatc agggccagta tcagttgccg gagccgccga
     3181 aactggctga cttcgaccag agcttgcagg actacaaagc acaggtacag gccgagatcg
     3241 cgcaggaggc cgccgcagcc ggtatgaccg tggaggaata cgcggcggct ggctatgaag
     3301 caccccaaca ggacaccttt tccatctacc agcttcgagg cggggaggaa acccgcgact
     3361 atcggtttga gccatacgag cgtttacagg ccgcagggct ggccgtggac agtgccaact
     3421 atgaccttgt ttatacggca ccgctggata aaagcacgtc tttggaggat atttaccgca
     3481 cattcaacat cgaccacccg gcagatttta ggggacacag cctttccgtt tccgacgtgg
     3541 tggtgctgca ccagaatgga caggacaccg cccactatgt agacagcttc ggctatatgg
     3601 aagtgccgga gtttttgcgg gaaaatcccc tgcgtaccgc cgaactgtct acggagcaga
     3661 acgaaaacat gattgacggc attatcaaca atacgccgcc cgaaccgcag ccggaggaac
     3721 ccgaacagct atcttttgag gatgtggcag ttgcttccgg ccagacacag gaggaacccc
     3781 cggaggctgc gcccccgcaa acggctgtcc gctattatcc catcaatgag ggagccgccc
     3841 gccgcgccaa ggaagccatg agcttttctg actaccggcc cggcagcgcc acggcggaat
     3901 accggcacta tgtcgatcag gccgtggaaa ttgcggagcg ccagaaaaag cgtgtggagc
     3961 cagagtatca cgaaaagata gacagcctcc ttgacaccta cgcccgcaag ctggccgaga
     4021 acatgaataa gggctatgag attaccgccc gcgtcccctc tgtcctgatt gccgggccgt
     4081 ccaatttccc ggtacgggcg aaagaaaagc agaacgccgc cagtgaccgc aacatggagg
     4141 aatttcagta cattcagggc ttgcttgaca aaatccgcag caccggcatg ggtggtatca
     4201 gcgccgacga tccgcaggcc gtttccaagc tggaaaagaa actggaaaag ctggaagcgt
     4261 cgcaggagtt gatgaaggcc gtcaatgcct actaccgcaa gcatgggacg ctggacggat
     4321 gcccccatct gatcgaaaga ggcattgaaa acctgaaagc ggatatggcg tcgggctggc
     4381 actatgagaa aaaaccgttc cagtcctggc agctctccaa caataacgcc gagatacgcc
     4441 ggttaaaggg ccgcattgag gaattgaccc gccaaaagga ggcggcctat gtgggatggg
     4501 agtttgacgg cggcactgtg gagatcaacc gggtggccaa ccgcctgcaa atcttctttg
     4561 aggggaagcc ggatgctgct gtacgcgacg agctgaaaag caacggtttc cgctggtcgc
     4621 ccaaggccgg ggcatggcag cggcagttaa acgacaccac aatccgcgtc gctgaccgta
     4681 tcaagtgcat ccagcccctt tccggcgaaa agcccagcgc cttacagctt gcggcccgca
     4741 gggagaaaga aaagccctct atccgggcgc agctcaacgc ggccaagacc gaacaggcca
     4801 agcagccacc ggcgcgggaa aaatcaaagg gattggaggt agagtaaatg cagaagtttg
     4861 aaaatgtgga cgttctggcc gcgctggaac agatcatgcg gcagaacacc gcgttttatc
     4921 aaagtgactt tggcattgac aaagacatta tcaggcggga tgccgccagc gatcaggccg
     4981 cagacaagac cctgctctgg atgtcccggc cctccgggac gtactgtttc cgggaacgtg
     5041 atgttttcct gaaagacacc cgacagtaca acacatggaa attttacggg gagcagaccc
     5101 gtgacaagat actggcctac gcggtggagc tgaccggcac acagggcggc actatccggg
     5161 gcaatctcta tgagctggac tatccgcagc atttccggca cgtcattgaa accgcccagc
     5221 cggtgagtgt gaaccggctc ttttatgagc atggcacccg tgacatcccg gagagccagc
     5281 gttttgacgg aagccctgac cgggtattgg gaaacttttt gtactatgag gcccagcccc
     5341 atgacccggc tgtgctgcag gaggcgctac ggacggagca gcacagccgg gggcgggccg
     5401 tccccggcga tttcaaagtc cacgtcaccg ctttgcatga cagccggatt gaaaccgagg
     5461 cccggcgtat tgtggacagc ctgaaagatc tgtccgcgcc taacagcccc aataaaacgc
     5521 atttcatggt ggagatctcc ccgcactttg cggcgattgc ttccaccaag gataatgacc
     5581 ggctctttgc catgctgccc tataaatccc tgtgttttac cgggatgaaa ggcaggcatg
     5641 gcgtgtatgc cgtgattaac cgggacgagc gccgggatgt gagtattcgc aggccccggc
     5701 cctctatccg caagcagcta tccgacagca aacaggccgc cagcccaaaa aaggcggcgg
     5761 ccagaaccaa aaagaatgaa ttggaggtat aaggaatgat gcactttacc gtggaggaag
     5821 aaaacctgat ctgtatgtac cacaatgcag accggcgcag aacgataaac aggctcaccg
     5881 ccgtcatgcc ggttatggat ggggatatgc gggcgcaggc ccagcagacc ctttccaagc
     5941 tggaacgcat gacggatgcc gactatgacg gccagaagtt ccacttcaca gacgaataac
     6001 gaacagcaga cgccgggcgc ggcggccatg tggtcgcttg cgtccggcgt cttttctttt
     6061 tgcctattcc tacgcattta gaacccggta ttgtcgggga taggatgaag tattcgcttg
     6121 cccctgcaaa catgcgggag aagccttgca gcgggcggtt ttcaatccct taaatgcgta
     6181 ggttgcagct ataattagac ccctgaaaca gatatagccg cgcagcgggt gtatctgttt
     6241 caggggtgtg caggtgatag ccgcaaagcg gcgtaagagg gtgcagccct cttgccgtct
     6301 atctctgttc cagcgggaag ctcggcaatg cttccagcag tttcagggaa atgcgctgcc
     6361 gtatatcttc gtcaatgact ttcttctttt tgccgtttgc ctgtctgatc tccaccgtgg
     6421 aaagctcgtt gatgtagtca gcataatggg ccaacacttt ttccgtggcc cacctctcac
     6481 cggcaacggc ggctttgatc gtttcatagt ccagcagctt ttcttgtccg tccatcctga
     6541 tacccccgtc agactgattt tttctatgat accatattcg gaagcatgga accagagaaa
     6601 acggcgcttt tctgctggta aattctacgc atttagaaca gcacttcggc ggggatagga
     6661 taaaacaccc acataccccg ctgaccatgc cggaaaagcc ttgaaatata agggctttta
     6721 cagctctaag tgcgtaggtt tccagtatct tttccgctgg cgctcggcct ctttgcggcg
     6781 gcgcatccgt ttggagcagg ccgcgcagta tttggcccgg ttagacttcg gcgtgaacgt
     6841 ggccccgcac tcacagcagc gtttcacttc gtcccggtct ttggtgatct cggcatacag
     6901 ggccgcatcc agcgggagga cagcggcctt aaaccagcgg cacagaagcg aataggaaat
     6961 gctctgtacg caaatacatt cttcgccgtc gtccagcaac aggcagttgc cgtcacaata
     7021 gttgcagcac tcatgcgtca gcttccgggc cttgcgatat tgagggtaat tcattcgtgg
     7081 aatactcata gctcttgcgt ccattccttt cccggcgtca tacgcaaaat gccgtccaca
     7141 ttctgcttga tgatgccgta ttcctgcatc tgctttttcg cttcgccgta cttctcatac
     7201 agccgttcct tttcctcggc caatcgcttg tactcggctt tcagggcgta cagatcgggg
     7261 agctttttca caccctgtcc ctttagggcc tttgccgcag cttcatacaa aatcaacgtg
     7321 ctttcatgct cacgccggaa ttttgcctta tccgctgcat tgcggtattc catagccacg
     7381 ggccagagct gcttgtaggt agaaatattc tttatcagca ccgccatatc cgcaagccgg
     7441 tgttccatag ctttgagggt agcggcggcc tcgtcgctgg cggcggtgat ctcggccact
     7501 ttgctttcca aatccgggta ggtgtctatc ccatgttcag tcaggaaatt caaagtcttt
     7561 gcggcctgct tcaaattgtg gagcttcgcc cacctctcat agcctgccga ctgctgggcc
     7621 ttaatgttgt tttccagatc aatccgcaaa gaaatcccct tgcgctcttt gggcgctctg
     7681 gtgataacgc ggcctttgat acgctctttg atggcttcct cggtgtaggc ttcccccaat
     7741 gtcttgcagc gggtaaagcg ttcctgtccg ggagcgcgga aagaaataaa ctttcccggc
     7801 ttgatctcat agccctgcgc ctgcatgagc cgcaggaaat catcaaaatc ttttgcctgc
     7861 gggatagcgg cgtcaatggc tgctttgagc ttcgctttcc agctcgtccc tttccgctgg
     7921 gcgtcccact cggcatagct cttgccctta ctctggccgg gcttcaccac ggacaatccg
     7981 ttttccttgc acagcctgtc gctggtgcgc cggatatagg catagctctg tttgttggaa
     8041 atgtacttgc gctgatttac catatccacg gcacagaaaa taatgtgatt gtggacgtgg
     8101 cccttatcaa tatgggtagt cagcacatag ggatatttcc cttgcagcac ttcctcggca
     8161 agctgcttcc cgatctcatg ggcctgttcc ggcgtggctt cgccgggggc aaaggattgt
     8221 atcaggtggt ggcccagatt attgcccttt tggtatgcct gcgccagcag catttcaaat
     8281 tcaatgtccg ctgtttcata agagcaggca aaggaggcca ccagcagctt gccgtcagtt
     8341 ttgtccgggt tctcgatgta gtcaagggct tttttcagcg ttgacttgat aggatgtatc
     8401 tttgtaactg ccatatctgc gccagcgccc cctttatatc ctcgatgtcc tgtgcgtaca
     8461 ccggccccat accgttcagc ctctttgcaa tctgattgac gttgacgccg atcttctgta
     8521 tctcggcggt ctgcgcccgg atgtcgctgg tgtccatgtt gacgatatag ccgtcaatga
     8581 gcatcttccg ggcgtaggct gaaaagttct tcgtatgcag cagcgccatt ttctgttcaa
     8641 tcatagcccg ttcctccggc gtcacaggga cgcgcagcac gatttttcgc tttctgtttg
     8701 ccatgcttca ccgtcctttc cattcagggg tttggggatt tccccaacaa gcaaaacgga
     8761 cgcggcccgg cagggacggg gcaggccgtt tttccgggag tggctacact ccctatgctt
     8821 gctacgttac tgtttctcca ctaacaagac gtttccaaag ccccaaacta cccgcaaaat
     8881 agccctctgg aaagaaaaaa gtgcaaaaaa atagaagcta cgctgtgcag ctcccgctaa
     8941 aagtaaaaca gaggatgcca gttacacatc ctccgtttca ttcttcttcg gcttttcaat
     9001 tagtatggcg ctgtcaatcg cgccctcgat gatcggcagg tattcttcgg ggcagagttt
     9061 cagcttgtgg ctgacccttt gccgcagttc gctttcctcc cgcatgatct ccgggttaaa
     9121 gtagcgttcc acaggaagcc cgcaggcttt aataagctgt atgacaaccg gcagactggg
     9181 aatggcccgt cgttttcgat gttggcaaga tagcgccagt caatatttat tttctctgcc
     9241 attgctttgc gcgaaacgcg ctttgcttgc cgggccgctt tcacatccgc gccgaaagtt
     9301 tcaaagccgg gacaatcttc aatattcgcc atatcgcacc tcccacttac attgtatatt
     9361 tcatactctg cccgtggaat gttggtatat gcacctttct taaagtttat atttcacatt
     9421 ttgtttcttt tattcttcga gcaaaagaat tataatgtac cctgtggagg tgtactatgg
     9481 actacatgac gttgaaagag gcaggcgaga aatggggtgt gcccccacga tggataaact
     9541 actattgtgc tgcgggacgc atccccggcg ctgtaaagat ggcgacgatc tggctcattc
     9601 ccaaaactgc ggaaaagccg attgacaggc gtacaaagaa aggaaaggcg gttactgaat
     9661 agtataatga aaccggcgca ttttggatat ggttttgaag agagagaaag catggtattt
     9721 tgtcacggaa aaactataag atgatagatg gcaggctgct ccagaccgat aaaaagtatt
     9781 ctcatctgaa acagaaagaa aagatttaca catggatgta tgaagaagcg aaactttatc
     9841 atgacagaaa tggaaaatgc ccgaaacaaa gacgaggaag ctgctgctgt atcagccgta
     9901 tatggtagaa ttgaaaaggc cggcatctgg attctttatg gtgaagtctc cagacattgg
     9961 ataactgctg gtctaatgcg cctttgagta tggggtgatt gattttaata ccggggaggt
    10021 acggcttttt gaggaaaata ttctgggatt tacatttggc gattattaca tttcctgtaa
    10081 ctgcaaggac acggatgatg aagatatgag gaatatagac ttgctgattt tctactgtct
    10141 tgaacggtat taggactgaa tctgctgcgc ggagtgcggc aagagcgacc ggagggagta
    10201 cacacaggcc ctgcctgtgt gaaaagccat accgcaaggt gcattcgcaa aaaagaggcc
    10261 gcgcattttg ctgaggcttg gggtgtcccc aacaagcaaa acgagaacgc cgcaggcggg
    10321 caagtggtgg atcagaatat ccgtaaagga tattctgatc cattgcttgc cagtattatc
    10381 gggatgtttt gacaaccgct gtaatcgaaa catcccaaac attaagaagg tgttaattat
    10441 tattatcttg atttaattcg tttaaaccaa taatatagat acatacacca tgaaaagaag
    10501 cgagtatccc aatgacggat gattaaaatc cgaaaagaaa tcgctttgaa actacaggga
    10561 aactacagac tgttatgtta tcttcttaaa atggagggat ttttatgtcg atacgaattc
    10621 tacttgtcga ggatgatgat catatctgta atacagtaag ggcgtttctg gctgaggcag
    10681 gatatcaggt ggatgcctgc acagatggaa atgaggcata caccaagttt tacgaaaaca
    10741 cttatcaact ggttattctt gatattatgc tgcccggtat gaacgggcat gaacttttgc
    10801 gtgaatttcg tgcgaaaaat gatactccca ttctgatgat gacagccctg tcggatgacg
    10861 aaaaccaaat ccgggcgttt gatgcagagg cagacgacta tgtaacaaag ccattcaaga
    10921 tgcagatttt actaaagcgg gtggaagccc tgctgcggcg cagcggtgcg ctggcaaagg
    10981 aaatccgtgt cggcaggctg acacttctgc cggaggattt cacggtactt tgtgacggta
    11041 cagagctgcc cctgacacga aaagaatttg aaatcctttt gctgctggtg cagaacaaag
    11101 gcagaacctt aacgcatgaa atcattttgt cacgtatatg gggatatgac tttgagggtg
    11161 atggcagcac agtccacact catatcaaaa atttgcgggc caagctgccg gaaaatatca
    11221 tcaaaaccat tcgcggtgta ggataccgat tggaggaatc ataataatgg aaagaaaagg
    11281 gattttcatt aaggtttttt cctatacgat cattgtcctg ctactgcttg tcggtgtaac
    11341 agcaacactg tttgcacagc aatttgtgtc ttatttcaga gtgatggaat tacagcaaac
    11401 agtaaaatcc tatcagccat tggtggaact gattcagaat agcgataggc ttgatattca
    11461 agaggtggca gggctgtttc actacaataa ccaatccttt gagttttata ttgaagataa
    11521 agagggaagc gtactctatg ccacaccaaa tgccaataca tcaaatagtt ttagacccaa
    11581 ctttctttat gtggtacata gagatgataa tatttcgatt gttgctcaaa gcaaggcagg
    11641 tgtgggattg ctttatcaag ggctgacaat tcggggaatt gttatgattg cgataatggt
    11701 tgtattcagc cttttatgcg cgtatatctt tgcgcggcaa atgacaacgc cgatcaaagc
    11761 cttagcggac agtgcgaata aaatggcaaa cctgaaagat gtaccgccgc cgctggagcg
    11821 aaaggatgag cttggcgcac tggctcatga tatgcattcc atgtatgtca ggctgaaaga
    11881 aaccatcgca aggctggagg atgaaatcgc aagggaacat gagttggagg aaacacagcg
    11941 atatttcttt gcggcagctt ctcatgagct aaaaacgccc atcgcggcta caagcgttct
    12001 gttggaggga atgcttgaaa atatcggtga ctacaaagat cattctaagt atctgcgcga
    12061 atgcatcaaa atgatggata ggcagggcaa aatcatttcc gaaatactgg agcttgtcag
    12121 cctgaatgat gggagaatcg tacccatagc tgaaccgttg gacatagggc gcacggttgc
    12181 cgagttgctg cccgattttc aaaccttggc agaggcaaac aaccagcggt tcgtcacaga
    12241 tattccagcc gggcaaattg tcctgtccga tccgaggctg ctccaaaagg cactatccaa
    12301 tgtcatattg aatgcagttc agaacacgcc gcagggaggc gaggtacgga tatggagtga
    12361 gcctggtgct gaaaaatgcc gcctttttgt tttgaacatg ggcgttcaca ttgatgatac
    12421 tgcgcttcca aggctgttca ccccattcta tcgcattgat caggcgcgaa gcagaaaaag
    12481 tgggcgaagc agaaaaagtg ggcgaagcgg tttaggactt gccatcgtac aaaaaacgct
    12541 ggatgccatg agcctccagt atgcgctgga aaacacctcg gatggcgttt tgttctggct
    12601 ggatttaccg ctcacatcaa cattgtaaat atttaaaact taaatgattt tgggctcttt
    12661 gtcaaatagg actgatagag tgagataaga acatctaagg tagaattcct tttctacttt
    12721 agatgttttt tgattcatag gtaatcagat tatttagtaa gaaaattctg gtcttcattt
    12781 tccgaaagct tcgaaagcca taagcgactc gtttcaatgc tttgatgtgg gtatgcagat
    12841 tttctatttt ggcatttgaa taaggcaatt cgagggctaa acgaatgccg gattcatagt
    12901 ttataaaagc tttttttata gaatgcagtt cttttggaac ggaattgggc atcgttctta
    12961 caagtttaaa gaaagtgtcc gcctctttgt cgtgaaacgc ttgaagaatt tcttgatagt
    13021 aaaaataggc gattcgtaat tcgtcagaga tcgaaagcag acgatccacc acttcacttt
    13081 cggtgagata cgcccaacca aaattgcgcc atttggtcca tttgtcataa acaagcttgt
    13141 cctgtcgttt gatcagaagc cgagacaacg cctttaattg tcgataatat ttggcttgtg
    13201 ttggatcgtt tcgatcaaag cttttcatga ttcgtacgcg aatatcctca aatttacgaa
    13261 tgagatgttt gacaatgtga aaacgatcca caacgagttt tgcctttgga aaacattcct
    13321 ttatcagaga ggcataagaa acattcatgt ccgtcacgat gacctttacc ttcaatcgtg
    13381 cgcgtcgttc aaattgcata aaataagaaa cgagagcctt tttcttccgt gaaggaagaa
    13441 tatccagtaa ttttccagta tcgccatcca tacaagaaaa ggagtaggga ccgacttgat
    13501 ttttcagtga acgaaattca tccattaata aacaacgcgg taaccaattt ctttttactt
    13561 ttgtcatggg gatcagagaa gcgagtgtcc gagaaacggt cgaccaagaa acgccgcaat
    13621 gcttagcaat tgtcgtcatt gcttgttttt cagttagctc ttctaaaatt ttatagcgca
    13681 cttgtttagc gataaagcag tttctatcga caatgtaggt attcgccgta aaataggtgt
    13741 ggcagccacg acagtggtag cgttgtttcg ctaaagcaag ataagtcagt tggttcgcac
    13801 atttattaag ttgaatgagc gattttttct ttccgttctt aacgatcatg ccttcatgat
    13861 tcatctgttt acacaaagga cagcgacgaa tttctttact taacgtcgca gaaacaacaa
    13921 gactcttgat attcttaatg ctcttcgatt ccacatttgt gatgaaaatg tttttatctt
    13981 tgattccaag tagtagtttg atatgatgaa gatcaggcat acagaacgct cctttgattg
    14041 tgttgtggtg attcaattct aacggagctt ctgtatgttt tgttttttca tgctcaaaag
    14101 ggaccgatag aatgattttc tatcagtcct agaaattata gagccgattt tgaccgacag
    14161 gtataaccct gccggtcttt ttttgctgct acaggaaaac tacagactgg ctacagggaa
    14221 agtacagatg cgcttgccat aataacaatc gtaccagcca caaatcgtag ttttattgca
    14281 aaggaggcat tcgatcaaat ggaaaaaagc aactatcatt ccaatgcgga tcatcacaaa
    14341 cggcatatga aacaatccgt ggaaaagcgg gcttttctat gtgcgttcat tatttcgttc
    14401 acagtctgca cgctgttttt ggggtggaga ctggcttccg tattggaggc aacacagata
    14461 ccgcccatcc ctgcaactca tacaggcagc agcactgacg tagtggagaa tttggaggaa
    14521 aacgctcttg ccaccgccaa agaacaggga gatgaacagg aatggagcct gattttagtg
    14581 aacaggcaga accccatccc cgcacagtac gatgtggagc ttgagcaact atcaaatggt
    14641 gagcggatag atattcggat ttctccctat cttcaagatt tgtttgatgc cgcaagaact
    14701 gatggagttt acccgattgt cgcatccgga taccgaacaa cagaaaaaca gcaagaaatt
    14761 atggatgaaa aaattgccga atataaggcg aaaggctaca cctctgcaca ggctaaagcg
    14821 gaagcagaaa cttgggtggc cgtgccggga acgagcgagc atcagcttgg tcttgctgtg
    14881 gatatcaatg cggacggaat tcattcaaca ggcaacgagg tttatagatg gctggatgaa
    14941 aacagctatc gttttggttt tattcgtcgc tacccaccag acaagacaga gataaccggt
    15001 gtgagcaacg agccgtggca ttaccgatat gttggcatcg aagctgccac agagatgtac
    15061 aaccaagggg tttgccttga ggaatattta aaaccagaaa aatgagaaaa ggatgttatg
    15121 ttatggacag aaaaagattg acacagcgtt tcccgttcct gcttccaatg agacgggcgc
    15181 agagaaaaat gtgcttttat gcgggaatga gatttgacgg ctgtcgctat gcgcagacga
    15241 taggggaaaa atcgctttcc catttgctct ttgaaacgga ttgcgcatta taca
//
LOCUS       DAYYME010000370         4610 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.guided.170, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000370 DAYYME010000000
VERSION     DAYYME010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.guided.170"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1014)
                     /locus_tag="ACHBFW_003072"
     CDS             complement(73..1014)
                     /locus_tag="ACHBFW_003072"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM9319280.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1140..2261
                     /locus_tag="ACHBFW_003073"
     CDS             1140..2261
                     /locus_tag="ACHBFW_003073"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM9319281.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            2519..4021
                     /gene="eat(A)"
                     /locus_tag="ACHBFW_003074"
     CDS             2519..4021
                     /gene="eat(A)"
                     /locus_tag="ACHBFW_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM9319282.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4088..>4610
                     /locus_tag="ACHBFW_003075"
     CDS             4088..>4610
                     /locus_tag="ACHBFW_003075"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9319283.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSY"
BASE COUNT         1497 a          838 c          992 g         1283 t
ORIGIN      
        1 tttttgatct tttattgggc ttaaaaaaag agaaccagct gctgattcag ctgattctca
       61 tcagagagcc ttttattctg cgccaaggat tccttctttt gcatggacta attgggtcaa
      121 aaaggcacaa tatggtgtag caatatcata tttttgtcct tttcgccaaa tggcaccatt
      181 gatgtagtcg atttcagtca aacgatggtt tttgatcagg tcttggtaca tagatgggta
      241 atgcaacccg atgttgtcag gatcataagt agaagcaata tgttggtaca cttcttcttg
      301 atctagtatg attccttctt ttgctgcgat agaagcaaat tcagatacga ttgttcgaac
      361 catgctctct gccgcttttt gcgcgcctag ttcagcaata ttacaatcca aaatcgtgca
      421 taaaccattc aatgttccat ttacacaagc ttttcgccaa atggaataac gaacgttatg
      481 gctatagatc ggatttaaat ttgcttcttg aaagacttct acaacttttt ttgtaaatgc
      541 ttcgccttct ggttccaagt tctctagttc gatttctcca tcacctaaca atgtgacttt
      601 tccgggacca gctagaccag ctgtccacat cgtgatccca aataagatat ttttctttgg
      661 cacatatttt tccagtacat cttcatgacc taatccattt agtaaacaca aaacataagt
      721 attgtcagta ataatggatt ggatcgaaca aaacatatca tctaattgat tggcttttgt
      781 taatgctacg atcaagtcca cgtgttcgtt gctttcgatg atttcttctg gtgaatagat
      841 tggcagtttt gcgaccactt ctttaccgtt aaagtcagca atcaacccat ttgtacgaat
      901 ggcttcaatg tgagcaggcc aacgatcgat caagaggacc tcatttccgc tttgatgtag
      961 catcaatccg attcggctgc ccattgctcc ggccccagct attgcgattt tcatgttttt
     1021 tctctccttt attcaatgag ttccctttta gtatatagaa aaatggaaga aaatagcatt
     1081 gttttgtttg aaaaaaatga aaaaaagtgt cttacttaag aagaaagtga gggatacaga
     1141 tggatatttt ggcggcaatt gattcattta aaggaagtgc ttcttcattt gaactaaatc
     1201 aggcagttct aaatggctgg gaagatcgta aaggaagaaa aatcaatgtg ccgattgcag
     1261 atggcggaga aaatactgca gaagcaatct attacgcgct caggggaaca tggcgtacca
     1321 ttgatgggat ggatctctta ttccgtcagc gaaaaatcag ctatttattg acaacttggc
     1381 aaggaaaaaa caccgcagtc atcgaatctg cacaagcatt ggggttagat ctacttgata
     1441 aaccaacgga tcaaactatc cgacttgctt ccagttatgc cttaggagaa cttattaaag
     1501 atgcactgct tcaagaagta gaacaaatta ttgtcacttt aggaggaagc gggtgtagcg
     1561 atggtggtct aggattactc caatccttgg gtgcttcttt agaagggtac acagaaggaa
     1621 atcctttatt atctactaaa aaacttcatt ttgatcgaag aaacgttctt gtttctaaag
     1681 taaaattgat tatcgctgca gatgttacca atccatatac aggtcaacaa ggagctgctt
     1741 ggcaatttgg aagacaaaaa ggaggaatag atacgacgct agctttctta gataaacaag
     1801 caggaaagat tgctcagcaa ataaaagatt tctgggaggt ggatttagat gatattccag
     1861 gaagtggagc agcaggcggt attggtggag cccttttttt gttgggagcg aagatggttt
     1921 ctggtttcga tttagtcagc caaatcactg gattggaaaa aaagatcgaa caagcagatg
     1981 tcatcattac aggagaaggg cggatcgatc agcaaacgat ccacggaaaa gttccatacg
     2041 gtgtagcagt gcttgcaaaa aaatatcgaa aaccaataat cgctatttgc ggaagtagag
     2101 accaagagct agaaggactt tctgattttc tcccaattgt cttaagtatt catttgggtc
     2161 caataggttt agaagaagca atggaacaca ggcaaacatt agaaaaaaca aaaatcttag
     2221 gacagacact tagcagattt ttcaccatcc tcccgaagta gttgacaaaa tcattttctt
     2281 tatgctatcg taatttcaaa tataggacaa agtgagggat ccgtgcattg aaaaattaag
     2341 tcgattgatg aaagctgcat tcagactgta ccttgtggag acaaggaagc agtgtgcaga
     2401 ttttcgggat tggcttggtt ttttcgatgt atgggtcttt ttgtgtgtat ccaacccttg
     2461 catcctcttt agccagttct gaaaatagga ctggtctttt tttacgaggt gatggaaaat
     2521 gtctaaaatc gaaataaaaa atctgacatt cggctacgac agccaaggca cattattatt
     2581 tgaacaagca aatctaaatt ttgacacaca atggaaacta ggacttatcg gacgaaacgg
     2641 tcgaggaaag acaactttac tgaatattct acaaaacaaa ctaccttatc aagggcaagt
     2701 aatccatcag caagaatttg cctatttccc gcaacagaca aaagataaag aacgtttaac
     2761 ctattacgtg ttaaatgata ttacggattt tgagatatgg gaaatcgaaa gagagctcca
     2821 attgatgcaa acagatcctg aaatcttatg gagagaattc agcacactat cggggggaga
     2881 gaagacaaaa gtcctactgg cacttttatt tgtggatgac actcatttcc cgttaatcga
     2941 tgaaccaacg aatcatttgg atatctctgg tagaaaacaa gtagcggctt atttgaaaaa
     3001 gaaaaaacaa ggcttcatcg tggtcagcca tgaccgggga tttatcgatg aagtagtgga
     3061 ccatgtttta gcaatcgaaa aaagtcaact ggaactttat caagggaatt tctctatcta
     3121 tgaagaacag aaaaaacttc gtgatgaatt tgaaatggct caaaatgaaa aattgaaaaa
     3181 agaagtcagt aggctaaaga aaacagcagc tgaaaaagcc gaatggtctc gttcccgaga
     3241 aggagataaa acaaagaaac aagtcggatt catcgatact gaatctagac gagtgaataa
     3301 aggagcagtg ggtgctgatg ctgcacggac gatgaaacga tccaaagcaa tcgtgaatcg
     3361 gatggagacc cagatcagcg agaaagaaaa actattaaaa gatatcgaat atatcgattc
     3421 tttgacgatg aatagccaag cgtctcacca taagcgactt ttaagcgtag aagatcttca
     3481 attagggtat gaaaatctgt tattcgagcc aattcatttt acaatcgagc ctcatcagcg
     3541 ggtggcgatt tcaggtccta acggtgcagg aaagtcatcc attatccatt atcttctggg
     3601 ggcattcaac ggcaaggtta taggagaaaa ataccagcca aaacatctga gcattagtta
     3661 tgcaagccaa aattatgaag acaatcgagg aacgttggcg gaatttgcag agaaaaacca
     3721 agtagactac caagcatttt tgaacaacct ccgaaagctt gggatggaaa gagatgtttt
     3781 tcataacaag atcgagcaga tgagtatggg ccaacggaaa aaagtggaat tggctaaatc
     3841 tttatcacag ccagctgaac tatatacatg ggatgaacca ttgaattatt tggatgtctt
     3901 caatcaagaa caattagaac aactgatctt gaacgtgaaa cctgccatgt tactagtgga
     3961 acatgatcaa accttcctgg ataaagtatc tactgagatt atttctcttg agagaatcta
     4021 acccttgtaa tctcaagaac aacaaggtat ggttaagggg aactttgcca acgaaggaga
     4081 gaatcacatg ctttatgtaa cgagacacgg agaaacaact tggaacgcac aaggattagt
     4141 ttgcggacgg gcagatgtgc cgctgactga aaaaggacag atgcaggcac agaaactagc
     4201 agaaaaggta gtagatttgc ctgttccaat cacgaaaatc atccattccc ctcttcaacg
     4261 agcaagagat acagcacaag cagttgctga tcgtctcagc ttgccgctga ccgttgatga
     4321 acgattggta gaaatggatt ttggggatta tgatggactg ccaagcaaag atgagaactt
     4381 tcaaaaagca cgtttagcgt tcgctgtacg attcccaaat ggggagtcag tcttagatgt
     4441 gtatgcacgg atcgttcctt tattgaaaga atgcatcgaa gacgaagaaa atgtctatct
     4501 gttagtttgc cataatgcct tgataagagt gatcaatgct tattttcatc cgatgcctaa
     4561 tgaggggttc tttacattca tggtagataa tacagaattg gtttcttatg
//
LOCUS       DAYYME010000371         3796 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.guided.194, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000371 DAYYME010000000
VERSION     DAYYME010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.guided.194"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..193
                     /locus_tag="ACHBFW_003076"
     CDS             110..193
                     /locus_tag="ACHBFW_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM9319284.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="ACHBFW_003077"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="ACHBFW_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM9319285.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1318..2181)
                     /locus_tag="ACHBFW_003078"
     CDS             complement(1318..2181)
                     /locus_tag="ACHBFW_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM9319286.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2183..2455)
                     /locus_tag="ACHBFW_003079"
     CDS             complement(2183..2455)
                     /locus_tag="ACHBFW_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM9319287.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2472..2681)
                     /locus_tag="ACHBFW_003080"
     CDS             complement(2472..2681)
                     /locus_tag="ACHBFW_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM9319288.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2759..3655)
                     /locus_tag="ACHBFW_003081"
     CDS             complement(2759..3655)
                     /locus_tag="ACHBFW_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9319289.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1174 a          662 c          586 g         1374 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa attttgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac cgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttat agatgtaatc
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt
     1081 ggaaagttac acgttactaa agggaatgta gataaattat taggtatagg gcacctctaa
     1141 taactaccaa ttaattcacc tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc
     1201 tatttcttat cgatacaaat tcctcgtagg cgctcgggac cccaacatga ggaaatgttt
     1261 ttataaaaaa agagtagtta ccgttttttg gtaactactc ttttcctttt ttaaggttta
     1321 aatacctgga agtttaggtg ttttgggtat tggtggtgta ggtagctgca agctttccat
     1381 tttttgttga attgctttaa attcaggggt ttcttggtgt tgattttgaa ccattttttg
     1441 ctctattctc tccaaagtcg gttgaatttc tttccctgat actttacgat ttaattctct
     1501 ttctaaggtc tctttcggac taatggaagg cgtttctaag cttgaataga gtttcactcc
     1561 ttctctattg taaagcctta tatcgctaaa taagcccgtt ttatgaaggg tttctaaatt
     1621 ggtcggtaag tttttgacaa caatatcatg cgcttgtttt ggtgttgccc tagctgtcat
     1681 tggatcatct gcatacatgg tttcatatcg ttcaatcgtt cctaagtatg attcaatttt
     1741 cggtactgcc atgacgtata tttttgtttc ataaccttta gattgaagca ttgtggctgt
     1801 ttgaatggga acgtctgttg ttcgtcctgt tccttcaatc actaaattat agccttgatc
     1861 gcttaaacgg ctaataagtg cttctgtcat gcgattcgaa taaggggtaa cgtgttttac
     1921 tacgtctttt tcatagagtt tcactaattc gtcaaaattt gggtgctgtt gtttaaaggt
     1981 atcattatca atgacaataa cattcccttg tgtttcttca aaaattgctg atcgcaaact
     2041 ggttttccct gaccctggtt gcccaccaag taaaaaagcg gttggcgatt caaccgctag
     2101 tctattttta gttagtcgtt caacattctt ttctaaacga tcttcaaatt gcttttcggt
     2161 gaagtcagta atattcgcca tgttaagcca ctttctcttt attcaaaatt tgttttgaat
     2221 attgattcat tgaccgccaa tactcatgaa tagcttgtaa ttcttcaaat ggttgttgaa
     2281 ataaatcaac ttcttgtgcc acttctaatt gatttaaaag attcacttct agtaaacgag
     2341 tatttttagt atctagttca tgattcaaaa cataatttaa tacccgatta taaacacttg
     2401 ccgataactc attaatgatt tcaatttcaa atgttttttc atacgtaact gccatttcat
     2461 tttcactcct tttaaagttt gtcaggtaaa tatttccgta tatattcttc taaggcttga
     2521 tccataactt cttttacgtt tccgccattc tttgctgttt cgatttttat gatatggtgc
     2581 aagtcggcac gaacacgaac cgtcttatcc cccattatat ctttttttgc gctgattggc
     2641 gtatcatttc gttttgcttt ctgtgcgcct aaatttccca cgatcgctca cttctttcta
     2701 tttcttctta ctatttattt tatcatcacc tatcacaaat cacaagtgat ttgtgattct
     2761 attctttttc attttctaat tgaatgattc gctcaatcat ttcaaaaaat acgttctcat
     2821 acatagacaa aacttttttg tcatagcctt tgtgttctgt aatgccattt ttagaccaag
     2881 tacttacttt attacttcgc ttgataatat ttcggaaaac caaattatcc tctttatgtt
     2941 ctttgtacag ttcttccagg tttgatttta tcgttgcgct gtccgtatca actaaataag
     3001 gaacaaaacc aatcatatct agtccagggt taaactgttc ttgcaaatca atcaaatagg
     3061 aaatatagtt ttgaatgttg tttgtacttt cttcttctgc ttgtaaaggg atcataacgt
     3121 aatcactcgc cacgattgca ttatttgtat aaacgcttgg cgttggtaca gtatcaataa
     3181 taataagatc atagtcactt tttaaaggtg ctaaaagagt agcaagcaat ctactttcat
     3241 tttcaaacgt ccatgagcga gttaattttg gcagtaacat caaatcaaac gtgccaggga
     3301 tcaagtctaa attatcagtc aaatgaataa tagaagaagc caagtttcca tttttcaagc
     3361 cttcataaaa attgacacgt ggcaattcta cctcaaaagt ttttgctaag tcttttgtca
     3421 atgttgcctg taagtcctta tcgatcatta aaacttttaa attcaatttg tctgtcaagt
     3481 aagcaaacat agtcgataat ttggactttc caacaccacc tttaaagtaa ttattcaaga
     3541 taataattgc ttcattttta ttgtttaaaa tacgtcttaa ttcttcaagt atttttagtt
     3601 tttccttctc cataactacg ccccattctt tttttgtata gtaatattgt atcattaaga
     3661 atcacaaatc acaagtgatt tgtgattaat cacttgtgat ttgtgataag tgatttgtga
     3721 ttaatatata aaagccctct ttaaagggct tttataggtt ctgttgcaaa gttttaaata
     3781 aagaataaaa tccctt
//
LOCUS       DAYYME010000372         3184 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.guided.209, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000372 DAYYME010000000
VERSION     DAYYME010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.guided.209"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..576
                     /locus_tag="ACHBFW_003082"
     CDS             <1..576
                     /locus_tag="ACHBFW_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFM9319290.1"
                     /translation="LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIR
                     PGETFSFWLFLHTPLTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDC
                     TYQIWVTLDDEKIIGQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGT
                     GEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            573..1544
                     /locus_tag="ACHBFW_003083"
     CDS             573..1544
                     /locus_tag="ACHBFW_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM9319291.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            1537..2565
                     /gene="vanB"
                     /locus_tag="ACHBFW_003084"
     CDS             1537..2565
                     /gene="vanB"
                     /locus_tag="ACHBFW_003084"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM9319292.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            2571..3179
                     /locus_tag="ACHBFW_003085"
     CDS             2571..3179
                     /locus_tag="ACHBFW_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM9319293.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT          879 a          667 c          875 g          763 t
ORIGIN      
        1 ctttcccatt tgctctttga aacggattgc gcattataca accacaatac cggatttgac
       61 atgatatacc aggaaaacaa ggtgttcaac ttaaagctgg cagcaaagac cttaaacggc
      121 ttattgataa gaccggggga aaccttttct ttctggttgt tcctgcatac gccattgaca
      181 attatccagc gcagaggtca cgaagtaaag gagtttccag agccaaacag tgacgagatt
      241 aaaggggtgg atgcaaccat ttcagagggc tggattgatt taaaagtgcg aaacgatacc
      301 gactgcacct accaaatatg ggtgacctta gatgatgaga aaatcatcgg tcaggtgttc
      361 gccgacaaag agccccaagc attatacaaa attacaaatg gcagtatcca gtatgtccgt
      421 gaaagtggcg ggatttatga atatgcccag gttaaacgga tgcaagttgc cttaggtacc
      481 ggggaaataa tagattgcaa gctgctttat acaaacaaat gcaaaatctg ttatcccctc
      541 ccggaaagtg tggatattca ggaggagaac caatgagaaa aagtgtgggc attactgttt
      601 ttggatgcga acaggatgag gcaaatgctt tccgcgcttt atcgccggat tttcatatta
      661 tccctacgct gattagcgat gcgatatcgg cagacaacgc aaaattggcc gctggcaatc
      721 aatgcgttag cgtaagccat aagtccgagg tttccgaggc gacaattctt gcgctgagaa
      781 aggtcggggt aaaatacatt tctacccgca gcatcggctg cgatcacatt gatacgactg
      841 ccgccgagag aatgggaatc tcggttggca cggttgcgta ttcgccggac agcgttgcgg
      901 attatgcttt gatgctgatg ctgatggcca tacggggtgc aaaacccacc atgcacgccg
      961 tggcgcaaca agatttcaga ttggatcgta tccgggggaa agaactgggg gatatgactg
     1021 tgggagttat tggaaccggc catatcgggc aagcggtcgt caaaaggctg cggggatttg
     1081 gatgccatgt gctggcctat gataacagcc gaaaaatgga tgcagattat gtccagcttg
     1141 atgagcttct aaaaaacagc gatattgtta cgctccatgt gccgctttgt gcggataccc
     1201 gccatctgat cggtcagaag caaattggag agatgaagca aggcgcattt ttaatcaaca
     1261 ccgggcgcgg agcgcttgtc gataccgggg cgctggtgga ggcgttggaa agcggaaagc
     1321 tgggtggtgc ggcactggat gtgttggagg gcgaggatca gtttgtttat accgattgct
     1381 cgcagaaagt gcttgaccat ccctttttgt cgcagctttt aaggatgccg aatgtgatca
     1441 tcacacccca tacggcgtac tacactgagc gtgtgctgca ggatactaca gaaaaaacaa
     1501 tcaggaattg tctcaatttt gaaaggagtt tacagcatga atagaataaa agtcgcaatc
     1561 atcttcggcg gttgctcgga ggaacatgat gtgtcggtaa aagccgcaat agaaattgct
     1621 gcgaacattg atacggaaaa attcgatccg cactacatcg gaattacaaa aaacggtgta
     1681 tggaagctat gcaagaagcc atgtacggaa tgggaagccg acagtctccc cgccatactc
     1741 tccccggata ggaaaacgca tgggctgctt gtcatgaaag aaagcgaata cgaaacacgg
     1801 cgtattgatg tggctttccc ggttttgcat ggcaaatgcg gggaggatgg tgcgatacag
     1861 gggctgtttg tattgtctgg tatcccctat gtgggctgtg atattcaaag ctccgcagct
     1921 tgcatggaca aatcactggc ctacattctt acaaaaaatg cgggcatcgc cgttcccgaa
     1981 tttcaaatga ttgataaagg tgacaagccg gaggcgggtg cgcttaccta ccctgtcttt
     2041 gtgaagccgg cacggtcagg ttcgtccttt ggcgtaacca aagtaaacgg tacggaagaa
     2101 cttaacgctg cgatagaagc ggcaggacaa tatgatggaa aaatcttaat tgagcaagcg
     2161 atttcgggct gtgaggtcgg gtgtgcggtc atggggaacg aggatgattt gattgtcggc
     2221 gaagtggatc aaatccggct gagccacggt atcttccgca tccatcagga aaacgagccg
     2281 gaaaaaggct cagaaaatgc gatgattaca gttcccgcag acattccggt cgaggaacga
     2341 aatcgggtgc aggaaacggc aaagaaagta tatcgggtgc ttggatgcag agggcttgcc
     2401 cgtgttgatc tttttttgca ggaggatggc ggcatcgttc taaatgaggt caataccctg
     2461 cccggtttta catcgtacag ccgctaccca cgtatgatgg ccgccgcagg aatcacgctt
     2521 cctgcactga ttgacagcct gattacattg gcgttaaaga ggtgataagt atggaaaatg
     2581 gttttttgtt tttagatgaa atgttgcatg gcgtccgttg ggatgccaag tacgccacat
     2641 gggataactt cacaggaaaa ccggtggatg ggtatgaggt gaatcgcatc atcggcacaa
     2701 aggccgtggc gtttgctctg cgcgaggcac aaatccatgc ggctgcgctt ggctatggct
     2761 tgcttttatg ggatggatat cggccaagaa ctgcggtgga ctgcttcctg cgttgggcag
     2821 cgcaaccgga ggacaatctc acaaaagaaa aattttaccc caatatagag cgagccgagt
     2881 tgattacaaa gggttatgtg gcctcacaat ccagccatag ccgtggaagc gcaattgatc
     2941 ttacgctcta ccacctggat acaggggaac ttgtttcaat gggaagtaac ttcgatttta
     3001 tggacgaacg gtcgcatcat acagcaaaag ggatagggga tgcagaggca caaaatcgaa
     3061 gatgcttgcg taaaatcatg gaaagcagcg gatttcagtc ttatcgcttt gaatggtggc
     3121 actataagtt gattgatgag ccataccccg atacctattt taattttgcc gtttcataat
     3181 gaaa
//
LOCUS       DAYYME010000373         3133 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.guided.212, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000373 DAYYME010000000
VERSION     DAYYME010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.guided.212"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..576
                     /locus_tag="ACHBFW_003086"
     CDS             112..576
                     /locus_tag="ACHBFW_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9319294.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            560..1189
                     /locus_tag="ACHBFW_003087"
     CDS             560..1189
                     /locus_tag="ACHBFW_003087"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM9319295.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1543..3021
                     /gene="msr(C)"
                     /locus_tag="ACHBFW_003088"
     CDS             1543..3021
                     /gene="msr(C)"
                     /locus_tag="ACHBFW_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM9319296.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3068..>3133
                     /locus_tag="ACHBFW_003089"
     CDS             3068..>3133
                     /locus_tag="ACHBFW_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM9319297.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLG"
BASE COUNT         1100 a          508 c          655 g          870 t
ORIGIN      
        1 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
       61 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
      121 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
      181 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
      241 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
      301 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
      361 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
      421 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
      481 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
      541 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
      601 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
      661 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
      721 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
      781 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
      841 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
      901 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
      961 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     1021 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     1081 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     1141 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     1201 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     1261 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     1321 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     1381 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     1441 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     1501 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     1561 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     1621 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     1681 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     1741 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     1801 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     1861 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     1921 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     1981 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     2041 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     2101 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     2161 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     2221 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     2281 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     2341 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
     2401 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
     2461 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
     2521 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
     2581 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
     2641 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
     2701 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
     2761 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
     2821 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
     2881 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
     2941 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
     3001 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
     3061 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
     3121 gatggtcttg ggg
//
LOCUS       DAYYME010000374         2050 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.guided.243, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000374 DAYYME010000000
VERSION     DAYYME010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.guided.243"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..488
                     /locus_tag="ACHBFW_003090"
     CDS             <1..488
                     /locus_tag="ACHBFW_003090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9319298.1"
                     /translation="DSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTA
                     VFNSNQSVLLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSH
                     EIAEVIMVLSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFC
                     K"
     gene            595..1833
                     /gene="mef(H)"
                     /locus_tag="ACHBFW_003091"
                     /gene_synonym="mefH"
     CDS             595..1833
                     /gene="mef(H)"
                     /locus_tag="ACHBFW_003091"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM9319299.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
BASE COUNT          659 a          282 c          359 g          750 t
ORIGIN      
        1 tcgattcaaa ggaagatatt ttaaaagcaa tttttcaaag aattggaaat gaaaatgcag
       61 taatatttgc taaaattaga gataacgata gtttaaacgg tttagaaaag ttaagagaaa
      121 tatttaagac agcggtattc aattctaacc aaagcgtttt attgacagtt tctcctcaac
      181 tattaaacaa tcctcgtttt cttgctatgc aaatagaaca aatgtatgaa attgtagtac
      241 ctgaatttgt tgaacccgtt ttagaacaag gtatcaagga taagtctttg gagattgaaa
      301 attcacatga aattgcagaa gttatcatgg tattaagtaa tgtatggctc aacccattag
      361 ttgaaatgac ggataaagag ggtatggaaa aacgttgcaa tacattcaat gcccttttaa
      421 atggaatagg catagacaca ttattagacg aagaaacaat ttctgcattt atacagtttt
      481 gtaaataact gtatttgtta tccctatacg atagactgct ctataaaaga gcagtttatt
      541 taaaccatta tataaaccga cggtctgttt gattaaataa aaaggagaga ttttatgcaa
      601 gcagaaaaaa aactttttaa tcgtgatttt atacttgttg ttataggaca gataatttct
      661 ttatttggaa acgctatcct acgatttgca ttaccgtact atttgttaaa agaaactggt
      721 tcatcagctt tgtttggtat tgctacagcc tgctcttttt tacctatggt tattttgtcg
      781 ctggtaggtg gtattattgc tgacaggaca aataaaagaa acattatggt catactagat
      841 tttgtaacag caatcataat tttagtattt tatttgtcat tgggaaaaat tcctatcata
      901 cctttgttta ttgttgcttt gatgttactt tatggtattt ctggtgcata tcaaccagcg
      961 gtacaagcaa gtattcctct attggtatca tcagataaac ttaccgtcgg taatgctata
     1021 ataaatcaag taaatacttt agcaaacttt atcggaccag ctataagcgg agtagttttt
     1081 caattttatg gtatcaatcc cattttaatt gttagtatta tatgttttac tttttcagcc
     1141 attatggaaa tttttatcca tattccacac caaaaaagac ctcgtgaagt aggtgtaatt
     1201 gcagttgcta aaaacgattt aaaagatagt tatacctttt tgaaaaagga gaaacccatt
     1261 tttatatccg taatcttttt ggtttgcatt ttcaatttgg ttttaagtgc tgttttaatt
     1321 gttggtctgc ctatcttaat ttctgaagtt ttaaaaatgc cagatacact atatggtttt
     1381 tcacaatcag cattatcatt aggtggttta tgtggtggta ttttaacagc tcttgttgct
     1441 tcaaagttaa aactaaaaaa cctttatacc ttgttactta tttgttctgt ttctgtttta
     1501 cttatgggaa tttcgctatt tttagaattg cctgcaatag tttcctactg ggtagttaca
     1561 gtgatgtgct ttgtggctat gggtgtatct actatgtttg ttgttcaaat ttacactctt
     1621 gttcagacac aaacaccacc gcaattagta ggaaagataa tggctacgct ggtagcagtt
     1681 gctatgtgcg gtcaacctat tggacaaatg gtttacggta ttttattcga tgtatttata
     1741 caagaaacat gggttgttat gtttgtagct tctattgctt caatcatgat ttcaatatat
     1801 tcaaaaagaa tttttcaaaa gttagagata taatacaagg ggttaaatag tatgttaggc
     1861 taaaaacaat atataaaaga aaatttgaaa tctgctaaaa ggatagtgtt ataaaagaaa
     1921 gagtaatatt gcagtattta aggctcgcac aaatatatgt tttgtgtggg cttttttctt
     1981 accatttttc tgtatttcta aagggtataa acggtattcc gtttatatat ttcagcatta
     2041 gcagaaaggg
//
LOCUS       DAYYME010000375          891 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.guided.305, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000375 DAYYME010000000
VERSION     DAYYME010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.guided.305"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..826
                     /gene="aac(6')"
                     /locus_tag="ACHBFW_003092"
     CDS             278..826
                     /gene="aac(6')"
                     /locus_tag="ACHBFW_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM9319300.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          318 a          129 c          186 g          258 t
ORIGIN      
        1 tatgaaaaca ggttttattt gtcaattcat aattggtttt ttttaaagca aaagataatc
       61 tatatcatga tgaacaaaca gagttttttc ctccaattga tggctttcct gatctttcct
      121 attttgaata aagtgtttct gtataatttt ttattttata agtaagataa ataatgaaag
      181 aatagagata ctgattggta gctaaagatt tttgagcgag aacatttcgt tttttcaaaa
      241 ggactgttag aataagcaag aacaaggggg aagacgtatg ataatcagtg aatttgaccg
      301 taataatcca gtattgaaag atcagctttc tgatttactg agactgactt ggccggaaga
      361 atatggagac agctcggcag aagaagtaga agaaatgatg aatccagaac gaatcgcggt
      421 agcagcggta gaccaagatg agttagtagg atttattggt gcaatccctc aatacggtat
      481 cacaggttgg gaattgcatc cattagttgt agaaagctcc cgacgaaaga accaaatagg
      541 tactcgatta gtcaattact tagaaaaaga agtagcttcc agaggaggaa tcacgattta
      601 tttaggtacg gatgatttag accatggaac aacgttaagt caaacggacc tgtatgagca
      661 tacatttgat aaagtggctt ccatccagaa ccttcgtgaa catccgtatg aattctatga
      721 aaaattaggt tataaaatcg taggtgtctt accaaatgca aatggctggg acaaaccgga
      781 tatttggatg gcaaaaacga ttattcctcg accaaattct caataataaa aagaggttgt
      841 gacagaagta atcagcttca agaaataaga aggaatttac gaaaattact t
//
LOCUS       DAYYME010000376          617 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.guided.328, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000376 DAYYME010000000
VERSION     DAYYME010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.guided.328"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..>617
                     /gene="dfrG"
                     /locus_tag="ACHBFW_003093"
     CDS             144..>617
                     /gene="dfrG"
                     /locus_tag="ACHBFW_003093"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM9319301.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVY"
BASE COUNT          233 a           61 c          129 g          194 t
ORIGIN      
        1 aaaatttgtg attttgtggt attatgataa tataacttat agaaaaaatt gcttattcaa
       61 ctaacgggca ggataatgag aaaaggtttt ttgatagaaa taagaaatta gatggtaaat
      121 tagttaaaaa ggctgtgtta taaatgaaag tttctttgat tgctgcgatg gataagaata
      181 gagtgattgg caaagagaat gacattcctt ggaggattcc caaggactgg gaatatgtta
      241 aaaatactac aaagggacat ccgataatat taggtaggaa gaaccttgaa tcaatcggaa
      301 gagccttacc tgacagaaga aatattattc tgacgagaga taaggggttt acctttaatg
      361 gttgtgaaat tgttcattca atagaagatg tttttgagtt atgtaaaaac gaagaagaaa
      421 tttttatttt cggaggagaa cagatttata atttgttttt cccttatgtt gagaaaatgt
      481 acatcacaaa aatacatcat gaattcgaag gagatacttt ttttccagaa gtgaattatg
      541 aggaatggaa tgaggtattt gcccaaaaag ggataaagaa tgataaaaat ccgtataact
      601 actattttca tgtatat
//
LOCUS       DAYYME010000377          574 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7097
            SAMEA115708802-rid22599293.guided.335, whole genome shotgun
            sequence.
ACCESSION   DAYYME010000377 DAYYME010000000
VERSION     DAYYME010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708802
            Sequence Read Archive: ERR13198522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290338.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290338.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,094
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,949
            CDSs (with protein)               :: 2,949
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 34 of 84
            Pseudo Genes (incomplete)         :: 37 of 84
            Pseudo Genes (internal stop)      :: 28 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708802-rid22599293.guided.335"
                     /isolate="VRE7097"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            92..441
                     /locus_tag="ACHBFW_003094"
                     /pseudo
     CDS             92..441
                     /locus_tag="ACHBFW_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT          191 a          106 c           83 g          194 t
ORIGIN      
        1 tttatactcc ttcttatatt tcctaataga gtataacata gctttaagca ttacttaaag
       61 ataacttaaa agttatcttt taaatactta attgttaaat accttatttg atgcaaaaga
      121 aatctacgat agtcaacaaa aaaattttca ttatatccag aaaaattttt cttagttaag
      181 gacctatgga ttgcagtaat gcagaattcg tcgaataaac tgccaaagac atacaatcat
      241 attgctttta aaattgacga acaagatata gattcgtttg tctcaaaaat ccaaatgtta
      301 ggtttaactg tcgaaccagg acgttctcgt gtgaaaggcg aggcttcttc aatttacttt
      361 tacgactatg ataatcacct cttcgagtta cataccggta ctctccagga acgtttaaaa
      421 tcctacaatt ccactacctg agactagtaa tgactagtct ctttttatgg cctctgatcc
      481 ccccattgag cgaatactaa tagggcttcg aattgatctg ttcaatcttt aagcaatatc
      541 cctcaaatga gtttggaaag tttagtgatt catc
//
